LOCUS       DAXWFK010000001        52718 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.001, whole genome shotgun sequence.
ACCESSION   DAXWFK010000001 DAXWFK010000000
VERSION     DAXWFK010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 52718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52718
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.001"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(87..338)
                     /locus_tag="ACGEXS_000001"
     CDS             complement(87..338)
                     /locus_tag="ACGEXS_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFF8011056.1"
                     /translation="MFGFIGMLIVGGLIGWAAGAIMGKDIPGGILGNIIAGIIGSWVG
                     GKLFGQWGPELGSIYILPALIGSIILIAIVTLILRAMRK"
     gene            638..901
                     /locus_tag="ACGEXS_000002"
     CDS             638..901
                     /locus_tag="ACGEXS_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8011057.1"
                     /translation="MNMHILYNLRTKHNLEIDELAQQLNEKYGTKYEAHQIWEWENHH
                     HEPKFKDAMHLADFFDAPYEMFLESKVKEYQKHLEEVDIRMDK"
     gene            complement(1039..1611)
                     /locus_tag="ACGEXS_000003"
     CDS             complement(1039..1611)
                     /locus_tag="ACGEXS_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFF8011058.1"
                     /translation="MKLKSLAVLSMSAVVLTACGNDTPKDETKSTESNTNQDTNTTKD
                     VIALKDVKTSPEDAVKKAEETYKGQKLKGISFENSNGEWAYKVTQQKSGEESEVLVAD
                     KNKKVINKKTEKEDTMNENDNFKYSDAIDYKKAIKEGQKEFDGDIKEWSLEKDDGKLV
                     YNIDLKKGNKKQEVTVDAKNGKVLKSEQDH"
     gene            complement(1792..2160)
                     /locus_tag="ACGEXS_000004"
     CDS             complement(1792..2160)
                     /locus_tag="ACGEXS_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFF8011059.1"
                     /translation="MKTILKTITYLVLTIIGAYAALFILKTIDSHGITDQFNPLVKKD
                     DSYVKTTEVSTRMDDQLRSYSQSVFNKEGKETQLMYTATFDVKPHRYLKITHKGHHVE
                     TFEEVEKEEVPKKALDKLSR"
     gene            complement(2529..3140)
                     /gene="selX"
                     /locus_tag="ACGEXS_000005"
     CDS             complement(2529..3140)
                     /gene="selX"
                     /locus_tag="ACGEXS_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin-like toxin X"
                     /protein_id="HFF8011060.1"
                     /translation="MFKKYDSKNSIVLKSILSLGIIYGGTFGIYPKADASTQNSSSVQ
                     DKQLQKVEEVPNNSEKALVKKLYDRYSKDTINGKSNKSRNWVYSERPLNENQVRIHLE
                     GTYTVAGRVYTPKRNITLNKEVVTLKELDHIIRFAHISYGLYMGEHLPKGNIVINTKD
                     GGKYTLESHKELQKDRENVKINTADIKNVTFKLVKSVNDIEQV"
     gene            3340..4047
                     /locus_tag="ACGEXS_000006"
                     /pseudo
     CDS             3340..4047
                     /locus_tag="ACGEXS_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505297.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            4086..5024
                     /locus_tag="ACGEXS_000007"
     CDS             4086..5024
                     /locus_tag="ACGEXS_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HFF8011061.1"
                     /translation="MLNFDEQIAKLKQMNIFFNIIDTEKANEILRKNNYFFKLAYFRK
                     NFEKKNGGYFIEFAYLSDLATIDMKLRYTMLHLTLDIEHSLKYLVLKLITENNQEDGY
                     KIIDEFLCIDKSYSNSNFDTNSRTPEEVMETKIKNKNEIFKHMNKRGQLPEKLNKYYQ
                     NPPAWVCIEFMQLGQFVSFLNFYYKKYNDEELRVANILMPLVKNIRNKSAHNQPIIAN
                     LNYDSRLPQYLFEKGNNIGISRNMFGIKNFIDTFATLELHNQVCSNAIIQARYHDLDQ
                     LQKRYKRNESYYNNALAIKRFFIALDKIIDFNRPKV"
     gene            complement(5290..5532)
                     /gene="rpsR"
                     /locus_tag="ACGEXS_000008"
     CDS             complement(5290..5532)
                     /gene="rpsR"
                     /locus_tag="ACGEXS_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002484306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFF8011062.1"
                     /translation="MAGGPRRGGRRRKKVCYFTANGITHIDYKDTELLKRFISERGKI
                     LPRRVTGTSAKYQRMLTTAIKRSRHMALLPYVKEEQ"
     gene            complement(5584..6087)
                     /gene="ssb"
                     /locus_tag="ACGEXS_000009"
     CDS             complement(5584..6087)
                     /gene="ssb"
                     /locus_tag="ACGEXS_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFF8011063.1"
                     /translation="MLNRVVLVGRLTKDPEYRTTPSGVSVATFTLAVNRTFTNAQGER
                     EADFINCVVFRRQADNVNNYLSKGSLAGVDGRLQSRNYENQEGRRVFVTEVVCDSVQF
                     LEPKNAQQNGGQRQQNEFQDYGQGFGGQQSGQNNSYNNSSNTKQSDNPFANANGPIDI
                     SDDDLPF"
     gene            complement(6108..6404)
                     /gene="rpsF"
                     /locus_tag="ACGEXS_000010"
     CDS             complement(6108..6404)
                     /gene="rpsF"
                     /locus_tag="ACGEXS_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFF8011064.1"
                     /translation="MRTYEVMYIVRPNIEEDAKKALVERFNGILATEGAEVLEAKDWG
                     KRRLAYEINDFKDGFYNIVRVKSDNNKATDEFQRLAKISDDIIRYMVIREDEDK"
     gene            6648..6839
                     /locus_tag="ACGEXS_000011"
     CDS             6648..6839
                     /locus_tag="ACGEXS_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011065.1"
                     /translation="MNLNIDWSKDFQEFQEILNSGIHPEWLYCAKANLVLEPAYTGEG
                     KQFFSTQDIINASKIIPFF"
     gene            complement(6925..8022)
                     /gene="ychF"
                     /locus_tag="ACGEXS_000012"
     CDS             complement(6925..8022)
                     /gene="ychF"
                     /locus_tag="ACGEXS_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFF8011066.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGALAANYPFATIDPNVGIV
                     EVPDARLLKLEEMVQPKKTLPTTFEFTDIAGIVKGASKGEGLGNKFLSHIREVDAICQ
                     VVRAFDDDNVTHVAGRVDPIDDIEVINMELVLADLESVEKRLPRIEKLARQKDKTAEM
                     EVRILTTIKEALENGKPARSIDFNEEDQKWVNQAQLLTSKKMLYIANVGEDEIGDDDN
                     DKVKAIREYAAQEDSEVIVISAKIEEEIATLDDEDKEMFLEDLGIEEPGLDRLIRTTY
                     ELLGLSTYFTAGVQEVRAWTFKQGMTAPQCAGIIHTDFERGFIRAEVTSYDDYVQYGG
                     ESGAKEAGRQRLEGKEYIMQDGDIVHFRFNV"
     gene            complement(8034..8237)
                     /locus_tag="ACGEXS_000013"
     CDS             complement(8034..8237)
                     /locus_tag="ACGEXS_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFF8011067.1"
                     /translation="MASKYGINDIVEMKKQHACGTNRFKIIRMGADIRIKCENCQRSI
                     MIPRQTFDKKLKKIIESHDDTQR"
     gene            complement(8267..9148)
                     /locus_tag="ACGEXS_000014"
     CDS             complement(8267..9148)
                     /locus_tag="ACGEXS_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFF8011068.1"
                     /translation="MNQVMNIISSLFEPLTKIETYENIATKIAMIVIYIIVALIVIKI
                     LNKMIEQGFKIQNKSKKSNKKRSKTLISLVQNVVKYIVWFIVITTILSKFGISVEGVI
                     ASAGVVGLAVGFGAQTIVKDVITGFFIIFESQFDVGDYVKINNGGTTVAEGTVKSIGL
                     RSTRINTISGELTILPNSSMGEITNYSITNGTAIVKIPVSVEENIDNVDKKLNKLFTS
                     LRSKYYLFVSDPVVIGIDAIEDTRVILRISAETIPGEGFAGARIIRKEVQKMFLQEGI
                     KTPQPIMTAYNHSENGV"
     gene            complement(9305..10150)
                     /locus_tag="ACGEXS_000015"
     CDS             complement(9305..10150)
                     /locus_tag="ACGEXS_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFF8011069.1"
                     /translation="MSELSKSEDQRITKTKDEQIKQIDISDIKPNPYQPRKTFDENHL
                     NDLADSIKQYGILQPIVLRKTVQGYYIVVGERRFRASKIAGLKYVSAIIKDLTDEDMM
                     ELAVIENLQREDLNAIEEAESYQRLMTDLKITQQEVAKRLSKSRPYIANMLRLLHLPK
                     KIADMVKDGRLTSAHGRTLLAIKDEQQMLRLAKRVVKEKWSVRYLENHVNELKNVSSK
                     SETDKVDITKPKFIKQQERQLREQYGTKVDISIKKSVGKISFEFDSQEDFVRIIEQLN
                     RRYGK"
     misc_binding    10379..10748
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10814..11917
                     /locus_tag="ACGEXS_000016"
     CDS             10814..11917
                     /locus_tag="ACGEXS_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HFF8011070.1"
                     /translation="MKDTQLAQITLTDDSTGAIANPIHLSTAYKHPKLGQSTGFDYTR
                     TKNPTRSTFETCFAKLEHGIASFATSSGMSAIQLICNLFKPHDEILVSFDLYGGTFRL
                     FEFYEQQYDIKFKYVDFTDYEQVEKEITDKTVALFIEPISNPQMIAIDVKPYYQLCKA
                     KGLLSIIDNTFLTPYLSTPLAEGADIVLHSATKYIGGHNDVLAGVVTVKDESLAQQLF
                     DFHNMTGATLSPIDSYLLLRGLKTLHLRIERAQSNARKLAKKCQSLQAIDEVLYSGQT
                     GMLSLRLNKAYSVAKLLENLDICIFAESLGGTETLVTFPYTQTHVDMPDAEKDKRGID
                     EYLIRLSLGVENYEDIERDIIQALDKAQIGEIV"
     gene            11914..13074
                     /gene="metC"
                     /locus_tag="ACGEXS_000017"
     CDS             11914..13074
                     /gene="metC"
                     /locus_tag="ACGEXS_000017"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase MetC"
                     /protein_id="HFF8011071.1"
                     /translation="MTLSKETEVIFDWRRGVEYHSANPPLYDSSTFHQTSLGGDVKYD
                     YARSGNPNRELLEEKLARLEQGKFAFAFASGIAAISAVLLTFKSGDHVILPDDVYGGT
                     FRLTEQILNRFNIEFTTVDTTKLEQIEGAIQSNTKLIYIETPSNPCFKITDIKAVSKI
                     AEKHELLVAVDNTFMTPLGQSPLLLGADIVIHSATKFLSGHSDLIAGAVITNNEAISD
                     ALYLIQNGTGNMLSAQDSWTLAKHLKTFPIRFKQSVENAQKIVSFLIKQDEISEVYYP
                     GLTTAHLEQAKNGGAVIGFRLADESKAQQFVDALTLPLVSVSLGGVETILSHPATMSH
                     AALPEEVRQERGITFGLFRLSVGLEDPDELIADIKYALKEAFNESIPHTIER"
     gene            13043..14884
                     /locus_tag="ACGEXS_000018"
     CDS             13043..14884
                     /locus_tag="ACGEXS_000018"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HFF8011072.1"
                     /translation="MSQFLTQLKDNVLVADGAIGTILYSEGLDTCPEAYNLSHPDKVE
                     RIHRSYIEAGADVIQTNTYGANFEKLKRFGLEDKVKAIHQAAVRIAKKAANKDTYILG
                     TVGGFRGIKQEDISLQTILYHTEIQIDTLIEEGVDALLFETYYDLEELTNVISRTRKK
                     YDIPIIAQLTASNTNYLVNGQAINEGLKQLVQCGANIVGLNCHHGPHHMQESFTHIEL
                     PEHAFLSCYPNASLLDIENSEFKYSDNAQYFGQVAQNLIREGVRLIGGCCGTTPEHIK
                     FIKESIQTLKPVNDKKVIPIPTKALFNPSQNKVRQSLTSKVQERPTVIIELDTPKHLD
                     TDRFFENIAKLDKANVDAVTLADNSLATVRISNIAAASLIKQYYNIEPLVHITCRDRN
                     LIGLQSHLLGLSLIGVNEILAITGDPSKVGHLPGATNVYDVNSKGLTELALRFNQGIN
                     TDGDALKKRTHFNIAGAFNPNVRKLYGAVKRLEKKIESGMSYFITQPVYSKEKIIEIY
                     HATKHLNKPFFIGIMPIASYKNALFLHNEVPGIKMSDEILQQFEAVKDDKAKTRELSL
                     KLSKDLIDTVHEYFNGLYIITPFQNVEDSLELAAYSKSITAHKEAIL"
     gene            14881..17109
                     /gene="metE"
                     /locus_tag="ACGEXS_000019"
     CDS             14881..17109
                     /gene="metE"
                     /locus_tag="ACGEXS_000019"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFF8011073.1"
                     /translation="MTTIKTSNLGFPRLGRKREWKKAIESYWAKKISKEELDQTLTDL
                     HKENLLLQKYYHLDSIPVGDFSLYDHILDTSLLFNIIPERFQGRTIDDDLLFDIARGN
                     KDHVASALIKWFNTNYHYIVPEWDNVEPKVSRNVLLDRFKYAQSLNVNAHPVIVGPIT
                     FVKLSKGGHQTFEEKVKTLLPLYKEVFESLIDAGAEYIQVDEPILVTDDSESYENITR
                     EAYDYFEKAGVAKKLVIQTYFERAHLKFLSSLPVGGIGLDFVHDNGYNLKQIEAGDFD
                     KSKTLYAGIIDGRNVWASDIEAKKVLIDKLLAHTNELVIQPSSSLLHVPVSLDDETLD
                     TSVGEGLSFATEKLDELDALRRLFNQNDSVKYDKLKARYERFQNQSFKNLDYDFESVR
                     TSRQSPFAQRIEQQQKRLNLPDLPTTTIGSFPQSREVRKYRADWKNKRITDEAYETFL
                     KNEIARWIKIQEDIGLDVLVHGEFERNDMVEFFGEKLQGFLVTKFGWVQSYGSRAVKP
                     PIIYGDVKWTAPLTVDETVYAQSLTDKPVKGMLTGPVTILNWSFERVDLPRKVVQDQI
                     ALAINEEVLALEAAGIKVIQVDEPALREGLPLRSEYHEQYLKDAVLSFKLATSSVRDE
                     TQIHTHMCYSQFGQIIHAIHDLDADVISIETSRSHGDLIKDFEDINYDLGIGLGVYDI
                     HSPRIPTKEEITTAINRSLQQIDRSLFWVNPDCGLKTRKEEEVKDALTVLVNAVKAKR
                     QE"
     gene            17152..17904
                     /locus_tag="ACGEXS_000020"
     CDS             17152..17904
                     /locus_tag="ACGEXS_000020"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HFF8011074.1"
                     /translation="MTQYPLWQQLNTLKQATWVDLTHTFDPEIPRFSEFEKGEVSTLF
                     TVKDHGFYVQRWNIVTQYGTHIDAPIHFVENKRYLEDIDLKELVLPLIVLDFSTEVAN
                     NNDFIVTRAHIEAWEKEHGTIEPGTFVALRTDWSKRWPNIEKFENKDANGQQHAPGWG
                     LDALKYLIEERRVEAVGHETFDTDASVDVVKNGDLVGERYILGQDKYQVELLTNLDQL
                     PTRGAIIYAISPKPKDAPGFPVRAFAIKPSND"
     gene            complement(17987..19168)
                     /locus_tag="ACGEXS_000021"
     CDS             complement(17987..19168)
                     /locus_tag="ACGEXS_000021"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFF8011075.1"
                     /translation="MTRVVLAAAYRTPIGVFGGAFKDVPAYDLGATLIEHIIKETGLN
                     PSEIDEVIIGNVLQAGQGQNPARIAAMKGGLPETVPAFTVNKVCGSGLKSIQLAYQSI
                     VTGENDIVLAGGMENMSQSPMLVNNSRFGFKMGHQSMVDSMVYDGLTDVFNQYHMGIT
                     AENLVEQYGISREEQDTFAVNSQQKAVRAQQNGEFDSEIVPVSIPQRKGEPIVVTKDE
                     GVRENVSVEKLSRLRPAFKKDGTVTAGNASGINDGAAMMLVMSEDKAKELNIEPLAVL
                     DGFGSHGVDPSIMGIAPVGAVEKALKRSKKELSDIDVFELNEAFAAQSLAVDRELKLP
                     PEKVNVKGGAIALGHPIGASGARVLVTLLHQLNDEVETGLTSLCIGGGQAIAAVVSKY
                     K"
     gene            19707..20840
                     /locus_tag="ACGEXS_000022"
     CDS             19707..20840
                     /locus_tag="ACGEXS_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HFF8011076.1"
                     /translation="MVNALTPKHLAEKRAGFHELFFDLIFVYAIQKIAHVILTTQNGS
                     ISADLFFKYIVMSLFLWLMWSHQTFFTNRFGQVTFKDVSFMMFNMFIMVFLSNSLYPD
                     FEKTFFPFFLCVAIMYLSIGLQYLLHIRTGLDYGDKRTCQAFASVAFVISFLSFLSLV
                     LPQSIHYIPGFLGVFIAATGLIPFQKYLVLSPVNMMHLVERFSLLTIIIFGEVLVGLA
                     SSSFSIDHFSYIYIFQFMILISLFGVYWIITENYINHKLTSIGFRLSYTHLLINIALG
                     VINAAIVFSNNNKLNDLFEINMMYISVLIFYIGLWLITPYFHNELTNAKYISYSLGIL
                     VMSYIVSLLFKGHDHVMIISVSVATFCIMLIYFKNQRLRNKHE"
     gene            complement(21207..21836)
                     /locus_tag="ACGEXS_000023"
     CDS             complement(21207..21836)
                     /locus_tag="ACGEXS_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HFF8011077.1"
                     /translation="MKGMFLSSFYATRKQTYIYFIVAIIAAGYFAVFNPLMSSAMAGV
                     MLITPITDNIKHEKDSRWMYYVSTLPVKRSDYIKSYFAFYLILFGASLMIGLVVTTIV
                     TQSVMIGIMSGLMSFGIIGAYSIIFPLTFKFGAENSNVIMICASILLLISFVVFFFIY
                     GMVSGASALEFEKISTEGWLVVIAYAVIGIVITSVSYILSIKIFNKQEL"
     gene            complement(21836..22678)
                     /locus_tag="ACGEXS_000024"
     CDS             complement(21836..22678)
                     /locus_tag="ACGEXS_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFF8011078.1"
                     /translation="MTTLLNVDSVNKQYKDSDFKLQDASLTISTNETVGLIGKNGSGK
                     STLINILVGNRHKDNGSITFFGEEHTVDDVEYKEHIGVVFDDLRVPNKLTIKDIDKVF
                     QSIYMTWNSQKFFDLIKYFELPLQTKIKTFSRGMRMKIALTIALSHDVKLLILDEATA
                     GMDVSGREEVMEILEDFVAQGGGILISSHISEDIEHLADKLVFMKDGRMILTEQKDIL
                     LAQYGIVTTEDKDVEIPKHLIIASRLSKGKYQILVKDYAEIENAEPLKHIDDATKIIM
                     RGEV"
     gene            complement(22703..23416)
                     /locus_tag="ACGEXS_000025"
     CDS             complement(22703..23416)
                     /locus_tag="ACGEXS_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HFF8011079.1"
                     /translation="MKILRYIGYLLLGGLVGGIIGGILGNFDGFGIENLTFATYNNVV
                     VISIVATIIIILVEAIVLMNQRRALKYKQLVDKEVDIDATDQYELLANRYVLNGSILS
                     ILQTVIAFLVLLIFVVGHAAANAILFFLIPFFASAIFNTQFTLFNRRFDDRIPKIADK
                     NYTEKRLEILDEGERHIELIALFKTYAINLSILILAIVVIGLYSITTGINQSFSLLLI
                     IAIFIYNAFSYLLKRRRFY"
     gene            complement(23413..23616)
                     /locus_tag="ACGEXS_000026"
     CDS             complement(23413..23616)
                     /locus_tag="ACGEXS_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8011080.1"
                     /translation="MRNRLKELRARDGLNQTQLAKQAGVSRQTISLIERNNFMPSVLT
                     AIKIARIFNETVETVFIIEEDEA"
     gene            23857..24246
                     /locus_tag="ACGEXS_000027"
     CDS             23857..24246
                     /locus_tag="ACGEXS_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFF8011081.1"
                     /translation="MAFTLSAIQQAHQQFTGVDFPKLFKAFKDMGMTYNIVNIQDGTA
                     TYVHQSEDDIVTSSVKSNHPVAQKSNKTIVQDVLTRHQQGQTDFETFCDEMAEAGIYK
                     WHIDIQAGTCTYIDLQDQAVISELIPQ"
     gene            24346..24570
                     /locus_tag="ACGEXS_000028"
     CDS             24346..24570
                     /locus_tag="ACGEXS_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HFF8011082.1"
                     /translation="MIIMITNTFILGITGPTSLVVISIIALIIFGPKKLPQFGRAIGS
                     TLKEFKSATEDLDKESHDTPSKESKQQREQ"
     gene            24587..25288
                     /gene="tatC"
                     /locus_tag="ACGEXS_000029"
     CDS             24587..25288
                     /gene="tatC"
                     /locus_tag="ACGEXS_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006191819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HFF8011083.1"
                     /translation="MVHFSELRHRLVKILLSFVITVIVVYVSSFWWMTPFITYITRAH
                     VSLHAFSFTEMIQIYVMIIFFIAFCFISPVMFYQLWAFIAPGLHNNERQFIYKYSFFS
                     VLLFCAGVAFAFYVGFPMIIQFALKLSLTLNISPVIGFKAYLIELIRWLFTFGLLFQL
                     PILFMGLAKFGLIDTTSLKHYRKYIYFACFVLASIIAPPDLTLNILLTLPLILLFEFS
                     MFIVKFTCRGKPPTH"
     gene            complement(25381..27093)
                     /locus_tag="ACGEXS_000030"
     CDS             complement(25381..27093)
                     /locus_tag="ACGEXS_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HFF8011084.1"
                     /translation="MKHYLTKFVAMLITAAMVCSFGLLKSQAAEQQSISDVYSVITDA
                     KSALSNNSISNDNKQKAIEQVVSAVKKLSLEDNSESNAVKSDVRKLEDAKANDNQKDT
                     LSQLTKSLIAYEEKLASKDAGSKIKLLQQQVDAKDAAMTKAIKDKNKAELESLNNSLN
                     QIWTSNETVIRNYDANQYGQIEVALLQLRIAIHKSPLDTAKVSHAWTTFKSNIDHVDK
                     KSNTSANDQYHVSQLNDALEKAIKAIDDNQLSDADAALTHFIETWPYVEGQIQTKDGA
                     LYTKIEDKIPYYQSVLDEHNKAHVKDGLVDLNNQIKEVVGHSYSFVDVMIIFLREGLE
                     VLLIVMTLTTMTRNVKDKKGTASVIGGAIAGLVLSIILAITFVETLGNSGILRESMEA
                     GLGIVAVILMFIVGVWMHKRSNAKRWNDMIKNMYANAISNGNLVLLATIGLISVLREG
                     VEVIIFYMGMIGELATKDFIIGIALAIVILIIFALLFRFIVKLIPIFYIFRVLSIFIF
                     IMGFKMLGVSIQKLQLLGAMPRHVIEGFPTINWLGFYPSYEPLIAQGAYIMVVAILIF
                     KFKK"
     gene            complement(27074..28303)
                     /gene="efeB"
                     /locus_tag="ACGEXS_000031"
     CDS             complement(27074..28303)
                     /gene="efeB"
                     /locus_tag="ACGEXS_000031"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFF8011085.1"
                     /translation="MTNYEQVNDSTQFSRRTFLKMLGIGGAGVAIGASGVGSMWSFKS
                     MFNTPEDPEKDAYEFYGKVQPGITTPTQKTCNFVALDLKSKDRDAIKAMFKKWTVMAD
                     RMMDGDTVGKPSNNPLMPPVDTGESIGLGASKLTITFGISKSLMKKIGLSSKIPDAFK
                     DLPHFPNDQLIDDYSDGDIMIQACSNDSQVSFHAVHNLVRPFRDIVKVRWAQSGFISA
                     KGKETPRNLMAFKDGTINPRKNNQLKDYVFIDDGWAKHGTYCVVRRIQIHIETWDRTA
                     LEEQEATFGRKRHSGAPLTGGKEFDEIDLKAKDSHGEYIIDKDAHTRLAKEANTSILR
                     RAFNYVDGTDDRTGNFETGLLFIAFQKATKQFIDIQNNLGSNDKLNEYITHRGSASFL
                     VLPGVSKGGYLGETLFD"
     gene            complement(28300..29154)
                     /gene="efeO"
                     /locus_tag="ACGEXS_000032"
     CDS             complement(28300..29154)
                     /gene="efeO"
                     /locus_tag="ACGEXS_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFF8011086.1"
                     /translation="MKKLTTLLLASTLLIAACGNDDSKKDDSKTSKKDDGVKAELKQA
                     TKAYDKYTDEQLNEFLKGTEKFVKAIENNDMAQAKALYPKVRMYYERSEPVAEAFGDL
                     DPKIDARLADMKEEKKEKEWSGYHKIEKALYEDKKIDDVTKKDAQQLLKDAKELHAKA
                     DTLDITPKLMLQGSVDLLNEVATSKITGEEEIYSHTDLYDFKANVEGAQKIYDLFKPI
                     LEKKDKKLSDDIQMNFDKVNQLLDKYKDNNGGYESFEKVSKKDRKAFADAVNALGEPL
                     SKMAVITE"
     gene            complement(29422..29967)
                     /locus_tag="ACGEXS_000033"
     CDS             complement(29422..29967)
                     /locus_tag="ACGEXS_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFF8011087.1"
                     /translation="MFGMKVNEQITLKILEAHDTEALFNLVNRSRNSLREWLPWVDAT
                     EQPSDTRAFIKRGLLQFADGNGFQCGIWYEGTLVGVIGLHEINHMHRKTSLGYYLDKE
                     FEGHGIMTQAVEALIKYCFEELDLNRIEISAAVNNEKSRAIPERLGFTREGMLRDNEL
                     LNGIYSSSYIYSLLKSEYDQK"
     gene            complement(30047..30265)
                     /locus_tag="ACGEXS_000034"
     CDS             complement(30047..30265)
                     /locus_tag="ACGEXS_000034"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011088.1"
                     /translation="MNNKVVIKLSCVIGNLDINQSNRHYNAYWRGPNKEADEKSAYNN
                     VQVGDGPQHREFRKEILQAMQVGEGQQI"
     gene            30330..31325
                     /locus_tag="ACGEXS_000035"
     CDS             30330..31325
                     /locus_tag="ACGEXS_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFF8011089.1"
                     /translation="MASLKNKHFMIGLSLTFIVALFSFLAAKLPILDKVGALTIAILI
                     AILYRHFRGYPEQYSSGITFSSKYLLRFAIILYGLKLNIFDIIGQGSKLLAIDVGVVI
                     FSIVMMLFVNKLLHGDKNIALLLGVGTGVCGAAAIAAVAPIFKSREKDTAISIGIIAL
                     IGTIFSLIYTAIYAIFSMTTNVYGAWSGVSLHEIAHVVLAGGFGGSDALKIALLGKLG
                     RVFLLIPLTIVLILIMRFRSSESSSKGRISIPYFLIGFVIMALVNTYVTIPSALLNIL
                     NTVSTICLLMAMVALGLNVAFKDLKNRALKPLMTIIITSICLSSLAFIVVHWLYS"
     gene            complement(31385..31951)
                     /locus_tag="ACGEXS_000036"
     CDS             complement(31385..31951)
                     /locus_tag="ACGEXS_000036"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFF8011090.1"
                     /translation="MDIVLLSGSTVGSKTRIAMDDLKNELEVINEGHQIELMDLRELE
                     LEFSVGKNYLDTTGDVYKLTTSLMQADVIFIGFPIFQASIPGALKNVFDLLPVNAFRD
                     KVIGLVATAGSSKHYLIPEMHLKPILSYMKAHTMQTYVFIEEKDFSNQQIVNDDVVFR
                     LKALAQSTMRTAKVQQQVFEEENNQYDF"
     gene            complement(31965..33026)
                     /locus_tag="ACGEXS_000037"
     CDS             complement(31965..33026)
                     /locus_tag="ACGEXS_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFF8011091.1"
                     /translation="MAKLEMNKNTPLEFGLYSLGDHLLNPLKGEKVSYEQRINEIIEA
                     SKLADEAGIDVFAVGESHQEHFTTQAHTVVLGAIAQATKHIKVSSSSTIISATDPVRV
                     FEDFATLDLISHGRAEIVAGRSSRTGIFDLFGYDLKDYDELFEEKLGLLLELNKTERI
                     TWSGKYRPELRNMKIFPRPIDNILPIWRAVGGPPASAIKAGKQGVPMMITTLGGPAMN
                     FKGSIDAYRQAATEAGFDASPKSLPVSTASLFYTAETTQDAMREFYPHLNTGMSFIRG
                     VGYPKQQFANSSDYREALMVGSPQQIIEKILYQHELYGHQRFMAQLDFGGVPFENVMK
                     NIELIGNDIIPAIKKHLSK"
     gene            complement(33040..33966)
                     /locus_tag="ACGEXS_000038"
     CDS             complement(33040..33966)
                     /locus_tag="ACGEXS_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFF8011092.1"
                     /translation="MNIVGHHHISMYTKDAKRNKDFYTNVLGLRLVEKSVNQDNPSMY
                     HLFYGDEVGTAGTILSFFEIPNAGHKQPGTETIYRFSLLVPNQAALHYFEKRLENNGI
                     KSERLYYLGQEGVVFKDEDDLEIILLVNDSFEVPHQWQHNAYSEIPQAYQILGIGPVE
                     LRVRNAARTVEFLENVLGYRKRDNKSFDVLTLAPQGLYSDFVVIEQQGQRERPGRGYI
                     HHIAVNTPQMSDLDAIYKKLQQQPQSNSGIIDRYFFKSLYYRHNSIMYEFATEAPGFT
                     IDTPVEQLGSQLNLPDFLEAEREQIESKLHEI"
     gene            34274..35632
                     /gene="glpT"
                     /locus_tag="ACGEXS_000039"
     CDS             34274..35632
                     /gene="glpT"
                     /locus_tag="ACGEXS_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFF8011093.1"
                     /translation="MNFLKPAKHIKPLPENQIDDTYKRLRLQVFLGIFIGYAGYYLLR
                     KNFSLAMPALQEQGFTKAELGFALSAVSIAYGFSKFFMGTVSDRSNARIFLVLGLALT
                     AIVNLLMGFVPFFTSGIGIMFVLLFLNGWFQGMGWPPSGRVLVHWFSVSERGSKTALW
                     NVAHNVGGGIMAPIAAWGITTTAFINFGYLKGFEGVFIYPALLALIIAAISYVLIRDT
                     PQSQGLPPIEIYKNDFATSDKKTLETELTTKEILFKYVLNNKWVWAIAFANIFVYFVR
                     YGVLDWAPVYLSEEKHFDLKASGWAYFLYEWAGIPGTLLCGYISDKLFKGRRGPAGFF
                     FMLGVTVFVLIYWLNPPGNAWLDNVSLIAIGFLIYGPVMLIGLQALDYVPKKAAGTAA
                     GLTGLFGYLFGAVMANIVLGAVVDKFGWDVGFILLTAISVFAMLSFILTWNKVGQETV
                     HH"
     gene            complement(35715..36155)
                     /locus_tag="ACGEXS_000040"
     CDS             complement(35715..36155)
                     /locus_tag="ACGEXS_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MepB family protein"
                     /protein_id="HFF8011094.1"
                     /translation="MCKSKILLKNIFSEESEVKDLTEEKYNQDYEALTFSFKEETYQS
                     RLAKKTPTKSGYFVTCWTKDEDNYNRPYKIEEFADYLIVAVIDDELNGYFLFPRELLV
                     EKGILASSKYQGKMAFRVYPKWCNQLNKTAGQTQKWQCKYFFEY"
     gene            complement(36259..37614)
                     /gene="mepA"
                     /locus_tag="ACGEXS_000041"
     CDS             complement(36259..37614)
                     /gene="mepA"
                     /locus_tag="ACGEXS_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498905.1"
                     /note="MepA (multidrug export protein A), as described
                     originally in Staphylococcus aureus, is a MATE transporter
                     shown to be over-expressed after culturing in the presence
                     of tigecycline, owing to mutation of its repressor MepR,
                     and then to be one of several factors contributing to
                     elevated tigecycline resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MepA"
                     /protein_id="HFF8011095.1"
                     /translation="MKDEQLYYFEKSPVFKAMMHFSLPMMIGTLLSVIYGILNIYFIG
                     FLEDSHMISAISLTLPVFAILMGLGNLFGVGAGTYISRLLGAKDYSKSKFVSSFSIYG
                     GIALGLIVILVTLPFSDQIAAILGARGETLALTSNYLKVMFLSAPFVILFFILEQFAR
                     AIGAPMVSMIGMLASVGLNIILDPILIFGFDLNVVGAALGTAISNVAAALFFIIYFMK
                     NSDVVSVNIKLAKPNKEMLSEIFKIGIPAFLMSILMGFTGLVLNLFLAHYGNFAIASY
                     GISFRLVQFPELIIMGLCEGVVPLIAYNFMANKGRMKDVIKAVIMSIGVIFVVCMSAV
                     FTIGHHMVGLFTTDQAIVEMATFILKVTMASLLLNGIGFLFTGMLQATGQGRGATIMA
                     ILQGAIIIPVLFIMNALFGLTGVIWSLLIAESLCALAAMLIVYLLRDRLTVDTSELIE
                     G"
     gene            complement(37721..38140)
                     /gene="mepR"
                     /locus_tag="ACGEXS_000042"
     CDS             complement(37721..38140)
                     /gene="mepR"
                     /locus_tag="ACGEXS_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor MepR"
                     /protein_id="HFF8011096.1"
                     /translation="MEFTYSYLFRMISHEMKQKADQKLEQFDITNEQGHTLGYLYAHQ
                     QDGLTQNDIAKALQRTGPTVSNLLRNLERKKLIYRYVDAQDTRRKNIGLTTSGIKLVE
                     AFTSIFDEMEQTLVSQLSEEENEQMKANLTKMLSSLQ"
     gene            38352..40307
                     /locus_tag="ACGEXS_000043"
     CDS             38352..40307
                     /locus_tag="ACGEXS_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFF8011097.1"
                     /translation="MLSYRQIEILYLLIHEQTFIPINTIANQLGVSPRTIQYDIAYIE
                     QYAETYHYQVSRNKAAGIKVTTAHATLLNELEHNLTNQIHFSKDERLTHIALKLFETT
                     DPVSTKQLAQDVNVSRRTIADDIKMIQAQLDQYHLKLNYVHNKGFNIIGEEDHYRKAY
                     AHFIHQYMKQAAPFIEADIFNSESIALVRRAIIKTLNSENYHLVQSAIDGLIYHILIA
                     IQRLNENFSFDIPINEIDKWRHTNQYAIASKMIENLERSCNVTFPESEIIFITLHLLG
                     SKMTEHTASSITFEYHDLSQNIHELITCVSQELGIDMSKDNKLHTSLITHIKPAIHRI
                     KYDMLQPNPLRQEVMRRYPQIIEAVSKHISPIEQDAAIRFNEDELTYITIHFASSIER
                     VATHKQSMIKVVLLCGSGIGTSQLLKSKLNHLYPEFHIWDAYSIYQLEESRLLQDNID
                     YVISTVPCEISAVPVIHVDPFINQQSRQKLNQIINDSREQRVMKMATDGKSLADLLPE
                     HRIIINKQPLSIESAIAVAVQPLINDGIVYSNYTAAILKQFEQFGSYMVISPHIALIH
                     AGTDYVQNGVGFALTYFTEGIIFGSKANDPVHLVITLATDHPNAHLKALGQLSECLSN
                     DLYRQDFLDGNIFKIKQHIALTMTKEA"
     gene            40312..40755
                     /locus_tag="ACGEXS_000044"
     CDS             40312..40755
                     /locus_tag="ACGEXS_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8011098.1"
                     /translation="MSLDILSTTRIIVKEQVNDWTEAITIASQPLLQEQIIEQGYVQA
                     MIDSVNELGPYIVIAPEIAIAHARPNNDVHQVGLSLLKLNQHVAFCDEDHYASLIFVL
                     SAIDNHSHLSVLQNLATVLGDNQTVQQLLTATNAQDIKNILKEHD"
     gene            40757..41041
                     /locus_tag="ACGEXS_000045"
     CDS             40757..41041
                     /locus_tag="ACGEXS_000045"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8011099.1"
                     /translation="MKILVVCGHGLGSSFMVEMNAQEALRQLNAPSDIEVEHSDIMTA
                     SPEMADLFICGRDLAENAERLGDVLVLDNILDKAELQQKLSEKLQQLNMI"
     gene            41056..42402
                     /locus_tag="ACGEXS_000046"
     CDS             41056..42402
                     /locus_tag="ACGEXS_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFF8011100.1"
                     /translation="MQAILNFIVDILSQPAILVALIAFIGLIVQKKPAATITSGTIKT
                     ILGFLILSAGADVVVRSLEPFGKIFQHAFGVQGIVPNNEAIVSLALKDFGTTAALIMV
                     CGMIVNILIARFTNLKYIFLTGHHTFYMAAFLAIILTVSHIKGWLTIVIGALVLGLIM
                     AVLPALLQPTMRKITGNDQVALGHFGSISYFAAGAVGQLFKGKSKSTEEIKFPKGLSF
                     LRESTISISITMALLYFIACLFAGVSYVHESISDGQNFIVFSLIQGVTFAAGVFIILT
                     GVRLILAEIVPAFKGISEKLVPNSKPALDCPIVFPYAQNAVLIGFFVSFITGVIGMFI
                     LFLFGGVVILPGVVAHFFLGATAAVFGNARGGIKGAIAGAALNGILITFLPLLFLPFL
                     GELGGAATTFSDTDFLAVGIVFGNAVKYMGLFGAILFIIIVGATTILLKGRQKEQQ"
     gene            complement(42495..43520)
                     /locus_tag="ACGEXS_000047"
     CDS             complement(42495..43520)
                     /locus_tag="ACGEXS_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFF8011101.1"
                     /translation="MNNKVLVTGGTGFVGMRIISRLLEQGYDVQTTIRDLSKADKVIK
                     TMQDNGISTERLMFVEADLSQDEHWDEAMKDCKYVLSVASPVFFGKTDDAEVMAKPAI
                     EGIQRILRAAEHAGVKRVVMTANFGAVGFSNKDKNSITNESHWTNEDEPGLSVYEKSK
                     LLAEKAAWDFVENENTTVEFATINPVAIFGPSLDAHVSGSFHLLENLLNGSMKRVPQI
                     PLNVVDVRDVAELHILAMTNEQANGKRFIATADGQINLLEIAKLIKEKRPEIAQKVST
                     KKLPDFILSLGAKFNHQAKEGKLLLDMNRNVSNERAKTLLGWEAIATQEEAILAAVDS
                     MAKYHLI"
     gene            complement(43835..44857)
                     /locus_tag="ACGEXS_000048"
     CDS             complement(43835..44857)
                     /locus_tag="ACGEXS_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFF8011102.1"
                     /translation="MYLIEPIRNGEYITDGAIALAMQVYVNQHIFLDEDILFPYYCDP
                     KVEIGRFQNTAIEVNQDYIDKHSIQVVRRDTGGGAVYVDKGAVNMCCILEQDTSIYGD
                     FQRFYQPAIKALHTLGATDVVQSGRNDLTLNGKKVSGAAMTLMNNRIYGGYSLLLDVN
                     YEAMDKVLKPNRKKIASKGIKSVRARVGHLREALDEKYRDITIEEFKNLMVTQILGID
                     DIKEAKRYELTDADWEAIDELADKKYKNWDWNYGKSPKYEYNRSERLSSGTVDITISV
                     EQNRIADCRIYGDFFGQGDIKDVEEALQGTKMTREDLTHQLKQLDIVYYFGNVTVEAL
                     VDMILS"
     gene            complement(44835..45779)
                     /locus_tag="ACGEXS_000049"
     CDS             complement(44835..45779)
                     /locus_tag="ACGEXS_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HFF8011103.1"
                     /translation="MQSSKWNAMSLLMDDKTKQAEVLRTAIDEADAIVIGIGAGMSAS
                     DGFTYVGERFTENFPDFIEKYRFFDMLQASLHPYGSWQEYWAFESRFITLNYLDQPVG
                     QSYLALKSLVEGKQYHIITTNADNAFDVADYDMTHVFHIQGEYILQQCSQHCHAQTYR
                     NDDLIRKMVVAQQDMLIPWEMIPRCPKCDAPMEVNKRKAEVGMVEDAEFHAQLHRYNA
                     FLEQHQDDKVLYLEIGIGYTTPQFVKHPFQRMTRKNENALYMTMNKKAYRIPNSIQER
                     TIHLTEDISTLITAALRNDSTTKNNNIGETEDVLNRTD"
     gene            complement(45769..46569)
                     /locus_tag="ACGEXS_000050"
     CDS             complement(45769..46569)
                     /locus_tag="ACGEXS_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HFF8011104.1"
                     /translation="METLKSNKARLEYLINDMHRERNDNDVLVMPSSFEDLWELYRGL
                     ANVRPALPVSDEYLAVQDAMLSDLNRQHVTDLKDLKPIKGDNIFVWQGDITTLKIDAI
                     VNAANSRFLGCMQANHDCIDNIIHTKAGVQVRLDCAEIIRQQGRNEGVGKAKITRGYN
                     LPAKYIIHTVGPQIRRLPVSKMNQDLLAKCYLSCLKLADQHSLNHVAFCCISTGVFAF
                     PQDEAAEIAVRTVESYLKETNSTLKVVFNVFTDKDLQLYKEAFNRDAE"
     gene            complement(46570..46902)
                     /locus_tag="ACGEXS_000051"
     CDS             complement(46570..46902)
                     /locus_tag="ACGEXS_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFF8011105.1"
                     /translation="MKKLANYLWVEKVGDLYVFSMTPELQDDIGTVGYVEFVSPDEVK
                     VDDEIVSIEASKTVIDVQTPLSGTIIERNTKAEEEPTILNSEKPEENWLFKLDDVDKE
                     AFLALPEA"
     gene            complement(46936..47937)
                     /locus_tag="ACGEXS_000052"
     CDS             complement(46936..47937)
                     /locus_tag="ACGEXS_000052"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFF8011106.1"
                     /translation="MVKLSVLDYALIDEGKDAQKALQDSVTLAKLADRLGFKRIWFTE
                     HHNVPAFACSSPELLMMHTLAQTNHIRVGSGGVMLPHYRPYKIAEHFRMMAALYPNRI
                     DLGIGNNPGTTMVKQALDGINPTYDSYDESISLLRDYLTIKDKPSAHTLGVQPHIDHF
                     PEMWLLSSSATSAKIAAELGIGLSVGTFLLPDINAIHAAKDNIDIYKKHFQASTIKMD
                     AKVMASVFVIVADNEAEVAALQHALDVWLLGKLQFAEFEDFPSVDTAQKYKLNDRDKE
                     MIQAHQARIIAGTQEKVKAQLDDFIATFEVDEVLVAPLIPGIEQRCKTLKLLAEIYL"
     gene            complement(48051..48143)
                     /locus_tag="ACGEXS_000053"
     CDS             complement(48051..48143)
                     /locus_tag="ACGEXS_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011107.1"
                     /translation="MESIFKIKLMNGICRSENMNMKKKNKGEKI"
     gene            48239..49438
                     /locus_tag="ACGEXS_000054"
     CDS             48239..49438
                     /locus_tag="ACGEXS_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFF8011108.1"
                     /translation="MYRYKPLLQSIHLPNGIKISNRFVLSPMTVNTSTKEGYITKEDL
                     AYAARRSNSAGMQVTGAAYIEPYGQLFEYGFNIDHDACIPGLTNMASTMKQHGSLAII
                     QLAHAGRFSNQAILNFGKVYGPSPMTLHSPIEHDVIAMSHEKINSIIQQYRDATLRAI
                     KAGFDGVEISIAQRLLIQTFFSTFSNKRTDHYGADSLKNRARLCLEVMRAVQEVIDKE
                     APDNFILGFRATPEETRGSDLGYTIDEFNQLINWVMDVSNIQYLAIASWGRHIYQNTS
                     RTPGDHFGRPVNQIVYEHLAGRIPLIASGGINSPESALDALQHADMVGMSSPFVTEPD
                     FVHKLAEQRPHDINLEFSMADLEDLAIPHAAFKDIVKMMDYGEGLKKHTRDALRQLEQ
                     NYHDSSS"
     gene            49511..50338
                     /locus_tag="ACGEXS_000055"
     CDS             49511..50338
                     /locus_tag="ACGEXS_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFF8011109.1"
                     /translation="MTEIKDKVITKDAFALPYTIIKAKNQPTKGAIVYIHGGGLMFGK
                     ANDLSPQYIDILTEHYDLIQLSYRLLPEVSLDCIIEDVYASFDAIQSQYSNCPIFTFG
                     RSSGAYLSLLIARDRDIDGVIDFYGYSRINTEPFKTTNSYYAKIAQSVNETMIAQLTS
                     PTPVVQDQIAQRFLIYVYARGTGKWINMINIADYTNSKYNIAPDELKTLPPVFIAHCN
                     GDYDVPVEESEHIMNHVPHSTFERVNKNEHDFDRRPNDEAITIYRKVVDFLNAITMM"
     gene            complement(50580..52652)
                     /gene="lip2"
                     /locus_tag="ACGEXS_000056"
     CDS             complement(50580..52652)
                     /gene="lip2"
                     /locus_tag="ACGEXS_000056"
                     /EC_number="3.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing triacylglycerol lipase
                     Lip2/Geh"
                     /protein_id="HFF8011110.1"
                     /translation="MLRGQEERKYSIRKYSIGVVSVLAATMFVVSSHEAQASEKTSTN
                     AAAQKETLNQPGEQGNAITSHQMQSGKQLDDMHKEDGKSGTVTEGKDTLQSSKHQSTQ
                     NSKTIRTQNDNQVKQDSERQGSKQSHQNNATNNTERQNDQVQNTHHAERNGSQSTTSQ
                     SNDVDKSQPSIPAQKVIPNHDKAAPTSTTPPSNDKTAPKSTKAQDATTDKHPNQQDTH
                     QPAHQIIDAKQDDTVRQSEQKPQVGDLSKHIDGQNSPEKPTDKNTDNKQLIKDALQAP
                     KTRSTTNAAADAKKVRPLKANQVQPLNKYPVVFVHGFLGLVGDNAPALYPNYWGGNKF
                     KVIEELRKQGYNVHQASVSAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKT
                     YKGIMPNWEPGKKVHLVGHSMGGQTIRLMEEFLRNGNKEEIAYHKAHGGEISPLFTGG
                     HNNMVASITTLATPHNGSQAADKFGNTEAVRKIMFALNRFMGNKYSNIDLGLTQWGFK
                     QLPNESYIDYIKRVSKSKIWTSDDNAAYDLTLDGSAKLNNMTSMNPNITYTTYTGVSS
                     HTGPLGYENPDLGTFFLMATTSRIIGHDAREEWRKNDGVVPVISSLHPSNQPFVNVTN
                     DEPATRRGIWQVKPIIQGWDHVDFIGVDFLDFKRKGAELANFYTGIINDLLRVEATES
                     KGTQLKAS"
BASE COUNT        16753 a        10073 c         7911 g        17981 t
ORIGIN      
        1 ataatacata ttgaccattt tcacgcaaat aaaaaagatg acaactgaat gccatcttta
       61 tttcaatttc caattgaatt tttttattat ttacgcatag ctcttaaaat taacgttacg
      121 attgcaatta agataattga accaattaat gctggcaaga tgtaaatact tcctaattca
      181 ggaccccatt gtccgaatag tttgccacct acccatgatc caataatacc tgcgataata
      241 ttgcctaaaa taccacctgg gatatcttta cccataatag caccagcagc ccatccaatt
      301 aagccaccga caattaacat tccaataaat ccaaacataa ttttcagtct cctttttcta
      361 tttattttgc gttattctaa gtagtacccc ttatttacaa ttctaaaaca aattcaaatt
      421 atttttatcc aaatattttt aaaagtagta attgaatatc aattttattc aatgtagcta
      481 tcgttattta aagtctctgt accgataata tcatatacat ttacattatt ttttctgccg
      541 aattcatagc ttgattattt tatgttatag gactagaata tacacatatt attagagcat
      601 ctttgaattt taaatcaaga agcgaggtta atgaacaatg aatatgcata ttttatataa
      661 cttacgaact aaacataatt tagaaattga cgaattagca cagcaattaa atgagaaata
      721 tggtactaaa tatgaagcac atcaaatttg ggaatgggag aatcatcacc atgaacctaa
      781 atttaaagat gccatgcatt tagctgactt ctttgatgca ccatatgaaa tgtttttaga
      841 aagtaaggtt aaagaatatc agaaacattt agaagaagtc gatattcgca tggataaata
      901 gatgcaaata aaccctcaca acacgtttgg catatatcct ttcaaatcta tactggatat
      961 attacattac gttgtgaggg tattttatta attaatatga attaagacat tttacaagcg
     1021 ttaatgcaac gaatcttttt agtgatcttg ctcactcttt aatactttac cgttcttagc
     1081 atcaacagta acttcttgtt ttttattacc ttttttcaaa tcgatattgt aaacaagttt
     1141 gccatcatct ttttcaagtg accattcttt aatatcacca tcaaattctt tttgtccttc
     1201 tttaatggct tttttgtaat ctatagcatc gctatattta aagttatcat tttcattcat
     1261 tgtatcttct ttttcagtct ttttgttaat cactttttta tttttatcag caacaagtac
     1321 ttctgactct tcaccagatt tttgttgcgt cactttataa gcccattcac cattagaatt
     1381 ttcaaatgaa attcctttca acttttggcc tttgtaagtt tcttcagctt ttttcacagc
     1441 atcttctggg cttgttttaa catcttttaa agcaataaca tcttttgttg tattagtgtc
     1501 ttgattagta tttgactctg ttgattttgt ttcatctttt ggagtatcat tgccacatgc
     1561 agtaagcacc accgctgaca ttgataacac tgctaatgat tttaatttca taatatcact
     1621 ctctcttcct atttttgaaa ctcataacaa aagcttatat gctatataga ttgtattacc
     1681 ccttgttttt aattttattc ataattatta caaatatttt taaattaatc gtcatgcgtt
     1741 actttcgttc gtactccttt tataaatgaa ccatgtaata taagcatgct attatcgact
     1801 cagtttgtct aatgctttct ttggtacttc ttccttttca acttcttcaa aagtttctac
     1861 atgatgacct ttatgtgtaa ttttcaagta tctatgcggt ttaacatcaa atgtagcagt
     1921 atacattaat tgcgtctctt tcccttcttt attaaaaaca ctttgactat aacttcggag
     1981 ttgatcatcc attctagtag acacctctgt cgttttaaca taagaatcat ccttctttac
     2041 taatgggtta aattgatctg ttataccatg ggagtctatt gtttttaaaa tgaataaagc
     2101 agcataagcg ccaatgatag taagtacaag atatgttatt gtttttaaaa tcgttttcaa
     2161 aagatcacct ctgacaaaat ataaaatagg atgtatctga aaacaactta cataattgta
     2221 attaaagtgt aagtattagg agaaaatgat ggtatttgaa tctgaaagat acgaatcgtt
     2281 tttaaactaa gttattttat agagacttta ttatgaaatt gaaagaagtt aattttaagg
     2341 ttgtcgaatg taaaaacata ttgattaacc ataaaagaga aattattttt gtagatttcg
     2401 gcataaacgt cattaaagaa ttttctatat gatggtgcta atcataacaa agaaagctag
     2461 gcacctttag atacctaact ttcaaagtat tagcgataaa acactatata tactaattta
     2521 gcttaatttc aaacttgttc aatgtcatta acacttttca caagtttgaa agttacattt
     2581 tttatatcgg ctgtattaat ttttacattt tccctatctt tttgtagctc tttatgcgac
     2641 tctaatgtat atttaccacc atcttttgta tttatgacga tgttaccttt aggcaaatgt
     2701 tctcccatat acaagccata ggaaatatga gcaaatctta tgatatgatc caattctttt
     2761 aaagtgacaa cttctttatt aagagtaata ttcctcttag gtgtatacac tctgccagca
     2821 actgtgtatg ttccttctaa atgtatacga acttggtttt catttaaagg tctctctgaa
     2881 taaacccaat tcctagattt attagatttt ccatttattg tatccttgct gtatctatcg
     2941 taaagttttt taaccaaagc tttttctgaa ttatttggta cttcttcaac tttttgtaat
     3001 tgtttatctt gtacacttga ggaattttgt gttgacgcgt ctgcttttgg atatattcca
     3061 aatgttcccc catagatgat acctagcgat agaatagatt ttaatacgat tgaatttttt
     3121 gagtcatatt ttttgaacat atttaattac ctccttgatg taaagcttta tttgctacaa
     3181 ttataaaaat aatagacgtg ttcatgaatt aaattcatct taactcttga ttaactttaa
     3241 tttgctacca ctctgaattt aataactata aatcgtctac acataattgg acaaaatcta
     3301 agagaataaa atttgttaat ttaaaatagc aagcaattca aagttatatg tgtaatagat
     3361 aaaatagata tccctatagt gatgcgttac tagctaaaca taataacaca ttagaagata
     3421 atgaagttaa ggagttactg gattgtttcg actatgtaat taagtataaa aatatccaac
     3481 gacaaaacgt aattataaaa tggtaaaagc tatggtacag tttcaaattg ctaatgacat
     3541 gcgtatcggt gagctacttg caataaagag agtaaatata aactatgaag ataaaacgct
     3601 agatatcgac ggtaaagtta attggataac tgaaaaaaga cgggagcatt cggagtaaag
     3661 gagacaactg aaagaagtaa tagctataag gccacagggc tcactaccca aagcatcgac
     3721 ttactaagaa cacttatgct tggaatgata agtttattga tagagagtac atattcacaa
     3781 atacgtctgg tagccctatc gactcgaaca aaattagcca cattattaaa ggggggcgct
     3841 gatattagtt ctattaagaa acctataacg acgcatacat tacatcattc gcatatatct
     3901 acacttgctc aattaggaat taacttaaaa gcaatgcaag agcatgtagg tcattcagat
     3961 tataaaaaaa atctagagat atacacacat gttactaatc agatggcgaa agatatgatg
     4021 aataaatttg aacgattggg gagttaaaat tggaaaaaga tgatacacta gcagaaatta
     4081 agcctatgct caattttgat gagcaaatag cgaaattaaa acagatgaat atatttttta
     4141 atattattga caccgaaaaa gcaaatgaaa ttcttagaaa aaataattac ttcttcaaac
     4201 tagcttattt ccgaaaaaat ttcgaaaaaa agaatggcgg ctatttcata gaatttgctt
     4261 atttatcaga tttagcaact atagatatga aattaagata cacaatgttg catttaactt
     4321 tagatattga acatagttta aagtatctag tcttaaaact aataacagaa aataaccaag
     4381 aagatggtta taaaataata gatgagttct tatgtattga taaatcatat agcaattcaa
     4441 attttgacac aaattcaaga acaccagaag aagttatgga aaccaaaatc aaaaataaaa
     4501 acgaaatatt caagcatatg aataaacgag gacaactacc cgagaagttg aataaatact
     4561 atcaaaatcc acccgcatgg gtttgcattg aattcatgca actaggtcaa ttcgtttcgt
     4621 ttctcaactt ctattacaag aagtacaatg acgaagaatt gagagttgct aatattttaa
     4681 tgcctttagt taaaaatata agaaacaaat cagctcataa ccaacccatc atagcaaatc
     4741 taaattatga cagtagatta cctcaatatt tatttgaaaa agggaataat ataggcatat
     4801 ctagaaacat gttcggaata aaaaatttca tagatacttt cgctacgcta gaattacata
     4861 atcaagtttg tagtaatgca attatccaag caagatatca cgatttggac caacttcaaa
     4921 agcgatataa aagaaacgaa agctattata ataatgcatt agctatcaaa agatttttta
     4981 tagctttaga taaaattatt gacttcaaca gaccaaaagt ataaactatc tagtgaggaa
     5041 agagacttat aggtctcgcg agttatttta attcgtatgc aagaaaaaga agagctatgc
     5101 attttattta aaatgcgtag ttcttttttt atgcatctaa attcatatta tttttgcaat
     5161 ataaacatat ctttgtgcaa attccgaaca caaaacattc acatcatcct tttttgccct
     5221 ttttctatac cccaaaacac aaaaagcccc gtaagcctat gcctacgggg tttgacaata
     5281 aattatatat tattgttctt ctttaacata tggtaataat gccatatgac gagaacgttt
     5341 gatagctgta gtcaacatac gttgatattt agctgaagta ccagttacac gacgtggtaa
     5401 aattttaccg cgttctgaga taaaacgttt taataattca gtgtctttgt agtcgatatg
     5461 tgtaatacca tttgctgtga aatagcatac ttttttacga cgacgtccgc ctcttcttgg
     5521 tccacctgcc atgattgtgt gcctcctttg ataatttttt cgctttaatt tcgttaattt
     5581 ttattagaat ggtaagtcat catcacttat atcaatcggt ccgtttgcat ttgcaaatgg
     5641 attatcagat tgtttcgtgt ttgatgaatt attgtacgaa ttgttttgtc ctgattgttg
     5701 accaccgaat ccttgaccgt aatcttggaa ttcattttgt tgacgttggc caccattttg
     5761 ttgcgcattt ttaggttcaa ggaattgaac gctatcacac acaacttcag taacaaacac
     5821 acgacgacct tcttgatttt cataattacg ggattgtaag cgaccatcta caccagctaa
     5881 actaccttta gataaatagt tatttacatt atctgcttgt cttctaaaaa caacacagtt
     5941 aataaaatct gcttcgcgct ccccttgagc attcgtgaac gtacgattta ctgcaagagt
     6001 gaatgtcgct acactcacac ctgagggagt ggttctgtat tccggatctt tcgttaaacg
     6061 acctactaat acaactctat ttagcattta aacgccccct ctaattatta cttgtcttcg
     6121 tcttcacgaa taaccatgta acgaatgata tcgtcactga ttttagctag acgttggaat
     6181 tcgtcagtag ctttgttgtt atcagattta acacgtacga tgttgtagaa gccatctttg
     6241 aaatcattga tttcataagc taggcgacgt ttaccccagt cttttgcttc taaaacttct
     6301 gcaccttcag tagctaagat accgttgaaa cgttcaacta acgctttttt agcatcttcc
     6361 tcaatgtttg ggcgtacgat gtacataact tcatatgttc tcattttata tttgcacctc
     6421 cttgtggtct atacggctta tcaatcttaa aacagataag caaggaataa ttttcattac
     6481 tcacaataaa gaattatatc atgcgccatt actttttaca ataataattc aaactactct
     6541 tcatatcatt tttgatatta attcatttga aactttctat gatattttta aaaatacact
     6601 tcacaaaagc gaacatatgt tctataatag ttgtgaggtg gtaaggaatg aatttaaata
     6661 ttgattggtc taaagatttt caagaattcc aagagatact taatagtggt attcatcctg
     6721 aatggcttta ttgtgcaaag gctaatcttg ttttagagcc tgcttatact ggcgaaggca
     6781 aacaattttt cagcactcaa gatattatta acgcgagtaa aattattcca ttcttttaat
     6841 aaattatacg cataattaaa agaggcgaac aattgattcc gcctcttcta catatatact
     6901 atttaattaa cttcactcta tcgtttatac attaaatctg aaatgaacga tatcgccatc
     6961 ttgcataata tattctttac cttctaatcg ttgtctgccc gcttctttag cgccactttc
     7021 gccaccatat tgtacatagt cgtcataact tgttacttcg gcacggataa atccacgttc
     7081 aaaatcagta tgaatgatac cagcacattg aggtgcagtc ataccttgtt taaatgtcca
     7141 agcacgtact tcttgcacac cagcagtaaa atatgttgat aatcctaata attcataagt
     7201 tgttctaatt aatcgatcta atcctggttc ttcgatacct aaatcttcta agaacatttc
     7261 tttatcttca tcatctaatg tagcaatttc ttcttcaatt tttgcactaa taacaatcac
     7321 ttcagagtct tcttgcgctg catattcacg aatcgctttt actttatcat tatcatcatc
     7381 accaatttca tcttcaccaa cattagcgat ataaagcatt tttttagaag tcagtaattg
     7441 cgcttgattc acccattttt gatcttcttc attaaagtca atactacgag cgggtttacc
     7501 attttctaaa gcttctttaa tagttgttaa aatacgtact tccatttcag cagtcttatc
     7561 tttttgacgt gctaattttt caattctagg caaacgtttc tcaacagatt ctaagtccgc
     7621 tagtactaat tccatattaa taacttcaat atcatcaata gggtctactc gaccagcaac
     7681 atgagttacg ttatcatcat caaatgcacg aacgacctga caaatcgcat ctacttctct
     7741 aatatgtgat aagaatttat tacctaaccc ttctcccttt gaagcacctt tcacaatacc
     7801 agcgatatct gtaaattcaa atgtagtcgg caatgtcttt ttaggttgaa ccatttcttc
     7861 taatttaagt aatctagcat ctggcacttc tactatccct acattaggat caatcgtagc
     7921 gaatggatag ttcgctgcta aagcacctgc ttttgttatt gcattaaata atgttgattt
     7981 accaacgttt ggcaatccaa cgatacctgc tgttaaagcc attaatcatt ctcctatctt
     8041 tgtgtatcat catgagattc gatgattttt ttaagttttt tatcaaacgt ttgacgtgga
     8101 atcataatac ttctttgaca attttcacat ttaattctta tgtctgcacc cattctaata
     8161 atcttaaaac ggtttgttcc acacgcatgt tgttttttca tttctactat atcatttatt
     8221 ccatattttg acgccattaa atatgacctc catgtattat aaactactaa acaccgtttt
     8281 cactatgatt ataagcagtc ataattggtt gaggtgtttt aataccttct tgtaaaaaca
     8341 ttttttgtac ttctttgcga ataattcgag ctccagcaaa tccttcacct ggaattgttt
     8401 ctgcagatat tctcaatatt actcttgtat cttcaatagc atcaatacca ataacaaccg
     8461 gatcactaac aaataagtaa tatttactac gtaaagaagt aaatagtttg tttagttttt
     8521 tatcaacatt atcaatgttt tcttcgacag acactggaat tttaacgata gctgtaccat
     8581 ttgtaattga gtagttcgtt atttcaccca tactactatt tggtaaaatt gttaattctc
     8641 ctgaaattgt attgattcgt gttgaacgaa gtccaattga tttaactgtt ccctctgcta
     8701 cagttgtacc accgttattt atcttaacat aatcacctac atcaaattgg ctttcaaata
     8761 taataaagaa tcctgtaatt acgtctttaa ctatagtttg agcaccaaaa cctactgcta
     8821 agcctacgac accagcactg gcaattaccc cttcaacact aatgccaaat ttacttaaaa
     8881 tcgtcgtaat aactataaac caaacgatat acttcactac attttgaaca agagatatta
     8941 aagttttaga gcgctttttg ttactctttt tacttttatt ttgaatctta aatccctgtt
     9001 caatcatttt attcagtatt ttaataacta tgagggctac gataatataa ataacaatca
     9061 tagcgatttt agttgcaatg ttttcatatg tttctatttt tgttaatggc tcaaatagag
     9121 atgaaataat attcatgact tgattcatga taagcgttac tcccttctat atgatatatc
     9181 aatattgtat tcatattttt atatacttcc aacctacgaa agtctcaata tatctagaat
     9241 caaatgttca ctgattacaa tttatttaca cttgcacaaa gagttattat ataaaattgt
     9301 gtaactattt accataccta cgatttaatt gttcaattat tctcacaaaa tcttcttgtg
     9361 aatcaaactc aaatgagatt ttaccaaccg atttttttat tgatatatct actttggtac
     9421 catactgttc tcgcaactgt cgttcttgct gctttataaa tttaggctta gttatatcta
     9481 ctttgtctgt ttccgacttt gacgaaacat tttttaattc attaacatgg ttttctaaat
     9541 atctgacact ccacttttct ttaacaaccc gtttcgctaa cctaagcatt tgttgttcat
     9601 ctttaattgc caataacgtt cgtccatgtg cacttgtcag tcgcccatct tttaccatgt
     9661 cagcaatctt tttcggcaaa tgtaataacc tcaacatatt cgctatatac gggcgcgact
     9721 tactcaatcg tttcgctact tcttgttgtg taattttcaa atctgtcatc aaacgttgat
     9781 aactttcagc ttcttcaatc gcatttaagt cttctcgttg taaattttcg atgaccgcca
     9841 gttccatcat atcttcatct gttaaatctt tgataatcgc tgatacgtat tttagaccag
     9901 caattttcga agctctaaac cttctttcac caactacaat gtaataacct tgaactgttt
     9961 ttctaagcac aattggttgc aaaattccat attgcttaat tgaatctgcc aaatcattta
    10021 aatgattttc atcgaaagtt tttcggggct gatacggatt cggtttgata tccgatatat
    10081 ctatttgctt aatttgttca tcttttgttt tagtaatacg ttgatcttca ctttttgaca
    10141 attcactcac atcacttttt atttgataac aacagttaaa tatattttat gtattctaaa
    10201 tgagtcagac aaccattaac cacactttat ttaaaaaata attcattttt caacttcaag
    10261 ataccaatta caatttcaca aacaaattaa cttttaaatc aaaattttct gaataattgt
    10321 tgacaaatct ttttttactc tgtaagctaa ttaatatctt ataacagttt aatgaaacgt
    10381 aaacacaata aagaggaaag taaaacacac cctgcttata cagagagtct ttagtagctg
    10441 agagaagatt ttgaaagcgt gtttgaaaat ggccttggag tgttgatgcc aatatgaggt
    10501 gtctacgggt tcgcccgtta tagcgataca gtattaacat tgatgttaaa tggcgtactg
    10561 gattctttac gcacgatttt ttgttaataa gtatgggata gcacattact atatccttac
    10621 ttactgactt taattgtgat aattgttcag taagcatatt tacttttaat gcgtactgaa
    10681 taaggttatt tcagcgatgg aataacaaat aaaggtggta ccgcgaaaca taagctttcg
    10741 tcctttttat ccgattcatt cgggtacgaa ggacggaagc tttttttatt ttttcttata
    10801 ggagggtctt aatatgaagg atacacagtt agcccaaatc acattaaccg atgattcaac
    10861 cggtgctata gcgaatccaa tccatttatc tactgcctac aagcatccaa aactaggaca
    10921 atcgacaggt tttgattata cacgtactaa aaatcctaca cgctcaacat ttgaaacctg
    10981 ttttgccaaa cttgagcatg gtattgcatc attcgctaca tcaagtggaa tgtcagccat
    11041 tcaattaata tgtaatctat ttaaacctca tgatgaaatt ttagtttcat tcgatttata
    11101 tggtggcaca tttagattat ttgaatttta cgagcaacaa tacgatatca aatttaagta
    11161 cgttgatttt acagattatg aacaagttga aaaagaaatc actgataaaa cagttgcatt
    11221 attcattgaa ccaatatcta acccacaaat gattgctatt gatgtaaagc catactatca
    11281 actttgtaaa gctaaaggct tattgtcaat tatcgacaat acttttttaa caccttatct
    11341 ttcaacacca ctagcagaag gtgctgatat agtcttacat tcagccacga aatatattgg
    11401 cggacataac gatgtactag caggtgtcgt aaccgtcaaa gatgaatcac tcgcgcaaca
    11461 gttgtttgat tttcacaaca tgactggcgc aacactttca ccaatagata gttatttgtt
    11521 gttacgtgga cttaaaactt tgcatttacg cattgagcgt gcgcaatcaa acgctagaaa
    11581 acttgctaaa aaatgtcagt cacttcaagc aattgacgaa gtactatata gcgggcaaac
    11641 tggcatgctt agtttaagac ttaacaaggc ctatagcgtc gctaaattat tagaaaattt
    11701 agacatttgc atttttgcag aaagtttagg aggtactgaa acattagtga ccttccctta
    11761 cacccaaaca catgttgata tgccagatgc tgaaaaagat aaacgtggca ttgatgagta
    11821 tttaatccgc ttatcactag gtgttgaaaa ttatgaagac atcgaacgcg atatcatcca
    11881 agcattagat aaagctcaga ttggagagat tgtatgacac tttcaaaaga gacagaagtg
    11941 atattcgatt ggcgtagagg tgtggaatat cattcagcta acccaccact ctatgattct
    12001 tcaacattcc atcaaacaag tcttggtggc gatgttaaat atgattatgc acgaagtggc
    12061 aaccctaacc gtgaactttt agaagagaag ttagcacgat tagaacaggg aaaattcgct
    12121 tttgcttttg catcaggtat tgctgctatt tcagcagtac ttttgacttt caaatctggt
    12181 gatcatgtca tcttacccga tgatgtatat ggcggtactt ttcgcctcac tgagcaaatt
    12241 ttgaatcgat ttaatattga atttacaacc gtagatacta caaagctcga acaaatcgag
    12301 ggtgccattc aatcaaacac aaaattaatt tatatcgaaa caccatcgaa tccctgtttt
    12361 aaaattactg atatcaaagc tgtttctaaa atagccgaaa agcatgaact actggtagct
    12421 gtggacaata catttatgac accgttaggt caatcacctt tattacttgg cgctgatatt
    12481 gtcattcata gtgctacaaa atttttaagt ggacatagcg atttaattgc tggtgctgtc
    12541 ataactaata atgaggcaat tagtgacgct ctttatttaa tacaaaatgg tacaggcaat
    12601 atgttatctg ctcaggatag ctggacactt gctaaacatt taaaaacttt tccaatcaga
    12661 tttaaacaat ctgtcgaaaa cgctcaaaaa atcgtgtcat ttttaataaa gcaagatgaa
    12721 atttcagaag tttattatcc gggactcact actgctcatt tagaacaagc taaaaatggc
    12781 ggtgccgtta ttggctttcg tttagctgat gagtctaaag cacaacaatt tgtcgatgca
    12841 ctgacattac cactcgtttc agtgagtctg ggcggtgttg aaacgatcct ttcacatcca
    12901 gcaacaatgt ctcacgctgc actacctgaa gaagtgagac aagaacgtgg tatcactttc
    12961 ggtctattcc gattaagtgt tggtctcgaa gatcctgatg aactcattgc agacatcaaa
    13021 tacgcattaa aggaggcatt caatgagtca attcctcaca caattgaaag ataatgtttt
    13081 agtagctgat ggcgctatag gaaccatttt atactctgaa ggattagaca cctgtccaga
    13141 agcatataat cttagccatc cagataaagt tgaacgcatc catcgttcat atattgaagc
    13201 cggtgctgat gtcattcaaa ccaatactta tggtgcaaat tttgaaaagt taaaacgatt
    13261 cggtcttgaa gataaagtta aagcaataca tcaagccgcc gttcgcatcg caaaaaaagc
    13321 agcaaataaa gatacgtata tattaggcac agttggtggg tttagaggta tcaaacaaga
    13381 ggatatcagc ttacaaacta ttctttatca tactgaaatt caaatagaca ccttaattga
    13441 agaaggcgtt gacgcgctac ttttcgaaac gtattacgac ctagaagagt taacaaatgt
    13501 catttcacga acgagaaaga aatacgacat tccaatcatt gctcaattaa ccgcttcaaa
    13561 cacaaattac ttagttaatg gtcaggcaat caatgaagga ttaaaacaac tcgttcaatg
    13621 tggtgcaaac atcgtgggac tcaattgtca tcatggcccg caccatatgc aagagtcttt
    13681 cacacatatt gaattaccag agcacgcatt cttatcttgt tatccaaatg ccagcttatt
    13741 agatattgaa aatagtgaat ttaagtatag tgacaatgca caatatttcg gtcaagttgc
    13801 tcaaaatcta attcgcgaag gtgttcgttt aattggtggt tgctgtggta caacgccaga
    13861 gcacatcaaa tttattaaag aatctattca gacacttaaa cctgttaatg acaaaaaagt
    13921 gattccgata ccaacgaaag cacttttcaa tccatctcaa aataaagtta gacaatcatt
    13981 aacatctaag gttcaagaac gtccaaccgt tattatcgaa ttggatacac cgaaacattt
    14041 agacacggat agattttttg aaaatatcgc taaacttgat aaagctaatg tagatgcggt
    14101 aacactcgca gataattcat tggcaactgt cagaattagc aatattgctg ctgctagctt
    14161 aattaagcaa tattacaata ttgaaccact cgtacatatt acatgtcgag accgaaactt
    14221 aatcggcttg cagtcccatt tacttggatt atcgctcatt ggcgttaacg aaatattagc
    14281 cataactggt gatccttcaa aagttggtca cttaccaggt gcaaccaatg tctatgatgt
    14341 taattctaaa ggattaactg aactcgctct aagatttaat caaggtatta acactgacgg
    14401 tgatgcgctg aagaaacgta cacacttcaa catcgctggc gcctttaacc ctaatgttcg
    14461 taaattatat ggtgccgtca aaagattaga gaaaaagata gaaagcggaa tgtcttattt
    14521 tataacacaa cccgtgtaca gcaaagagaa aatcattgaa atttaccatg ccactaagca
    14581 cttgaacaaa ccatttttca taggcattat gcctatcgca agttacaaaa acgcactctt
    14641 tttgcataat gaagtgccag gaatcaagat gtcagatgaa attttacaac aatttgaagc
    14701 agttaaagat gataaagcca aaacacgaga actaagtctt aagctttcaa aggatttaat
    14761 cgatactgtt catgaatatt ttaatggttt atacattatc acaccgtttc aaaatgtcga
    14821 agattcatta gaacttgcag catactcaaa atctattact gctcacaagg aggcaatatt
    14881 atgacaacaa ttaaaacatc aaacttagga ttcccaagat taggtagaaa aagagaatgg
    14941 aaaaaagcca tcgaaagtta ttgggcaaag aagatttcta aagaagaatt agatcaaaca
    15001 ttaactgatc tacataaaga aaatttatta ttacaaaagt actatcactt agatagcatc
    15061 ccagttggtg atttctcttt atatgaccat atattagata cgtcattatt attcaacatc
    15121 atccctgaac gtttccaagg aagaactatt gatgatgatt tattatttga tattgctcgc
    15181 ggtaataaag accatgttgc aagtgcactt ataaaatggt ttaacactaa ctatcattac
    15241 atcgtacctg aatgggacaa tgttgagcct aaagtgagtc gcaatgtatt attagatcgc
    15301 tttaaatatg cccaatcttt aaacgttaac gctcaccctg tcattgttgg tccgattact
    15361 tttgttaaat tatcaaaagg cggtcaccaa acttttgaag aaaaagttaa aacgttatta
    15421 ccactttata aagaagtgtt tgaatcatta attgatgcag gtgctgagta cattcaagtt
    15481 gatgagccaa tcttagttac agacgacagc gaaagctatg aaaatattac acgtgaagct
    15541 tatgactatt tcgaaaaagc tggtgttgct aaaaaattag tcattcaaac atactttgaa
    15601 cgtgcacatc ttaaattctt aagttcttta cctgttggtg gtataggttt agactttgtc
    15661 catgataatg gctataacct taaacaaatt gaagctggag attttgataa atcaaaaaca
    15721 ttatacgctg gaattattga tggtcgtaat gtatgggcaa gtgacattga agctaaaaaa
    15781 gtcttaatcg ataaattgtt agcacacact aatgaacttg tcattcaacc atcatcttca
    15841 ttattacatg ttccagtatc tttagatgat gaaacattag atacaagtgt tggcgaaggc
    15901 ttaagctttg caactgaaaa attagacgaa ttagatgcat tgcgtcgcct atttaatcaa
    15961 aacgatagtg ttaaatatga taaattaaaa gcacgttatg agcgtttcca aaatcaatca
    16021 ttcaaaaact tagattatga tttcgaaagc gtgcgtactt caagacaatc accattcgcg
    16081 caacgtattg aacaacaaca aaaacgtttg aacttaccgg atttaccaac aacaactatt
    16141 ggatcattcc cacaaagccg agaagttcga aaataccgtg cagattggaa gaacaaacgc
    16201 attacagacg aagcatatga aacattctta aaaaatgaaa ttgctcgatg gattaaaatt
    16261 caagaagaca ttggcttaga tgtattagtt cacggtgaat ttgaacgtaa tgacatggtt
    16321 gaattcttcg gagaaaaatt acaaggtttc ttagtaacta aattcggttg ggtgcaatca
    16381 tatggttcac gtgccgtaaa accaccaatc atttatggtg atgtaaaatg gacagcgcct
    16441 ttaactgttg atgaaacagt ttatgcacaa agcttaacag ataaaccagt taaaggtatg
    16501 ttaactggac ctgtaacaat tctaaactgg tcatttgaac gtgttgattt accacgtaaa
    16561 gtcgttcaag atcaaattgc tttagcaatc aacgaagaag tattagcact tgaagctgca
    16621 ggaatcaaag ttatccaagt tgacgaacct gcattacgtg aaggcttacc attacgctct
    16681 gaatatcacg aacaatatct taaagatgct gttttatcat ttaaacttgc aacgtcttca
    16741 gttcgtgatg aaactcaaat ccatacacat atgtgttatt ctcaattcgg tcaaatcatt
    16801 catgctattc atgacttaga tgctgatgtt atttcaattg aaacatctcg tagccatggt
    16861 gatttaatta aagactttga agatattaat tatgatttag gtattggtct aggtgtatat
    16921 gatattcata gtccacgtat tccaacaaaa gaagaaatta ctacagcaat caatcgttca
    16981 ctacaacaaa tcgaccgctc attattctgg gtaaaccctg actgtggttt aaaaacgcga
    17041 aaagaagaag aagttaaaga tgcattgact gtgcttgtga atgctgttaa agctaaacgc
    17101 caagaataat cgcagcattt atcatataat aaactaaaaa cgggtgattt tatgacacaa
    17161 tatcctttat ggcaacaatt aaatacttta aaacaagcaa cttgggttga tttaacgcat
    17221 acatttgatc cggaaatacc acgttttagt gagtttgaaa aaggcgaagt ttcaacttta
    17281 ttcacggtta aagatcatgg tttctatgtg caacgttgga atattgtaac gcaatatggt
    17341 actcacattg atgcaccaat tcactttgtc gagaataaac gttatttaga agacattgat
    17401 ttaaaagaat tggtattgcc gttaattgta ttagactttt caacagaagt agcgaacaac
    17461 aatgatttca tcgtaacacg tgcgcatata gaagcatggg aaaaagagca tggtactatt
    17521 gaacctggca cttttgttgc acttcgtacc gattggtcaa aacgttggcc taatattgaa
    17581 aagtttgaaa acaaagatgc gaacggacaa caacatgcac cgggttgggg attagatgct
    17641 ttgaagtact taattgaaga acgtcgtgtt gaagctgttg gtcatgaaac attcgatacc
    17701 gatgcatctg tggacgttgt aaaaaatggt gatttagttg gcgaacgtta tattcttggt
    17761 caggataaat accaagtcga gttattaact aatttagatc aattaccaac tcgtggcgcc
    17821 atcatttacg ctatcagccc taaaccaaaa gatgcacctg ggttccctgt tagagcattt
    17881 gcaattaaac catcaaatga ctaatattac ccaataaatc cataaacaaa taaaaacagg
    17941 ttatgccaac atgtaattaa tactgttgtg ataacctgtt ttcttattat ttatactttg
    18001 atacaactgc agcgatagct tgaccgccac caatacacaa tgatgttaaa ccagtttcaa
    18061 cttcatcatt cagttgatgc aataatgtca ctaatactct agcaccagat gcaccaatag
    18121 gatgtcctaa tgcaatagcg ccacctttaa cattcacctt ttcaggaggt aattttaatt
    18181 cacgatcaac agctaatgat tgtgctgcaa atgcttcatt taattcaaat acatcaatat
    18241 cgcttaattc ttttttacta cgtttcaaag ccttttctac agcgccaact ggtgcaatac
    18301 ccataataga aggatctaca ccatgacttc caaagccatc aagcactgcc aatggttcga
    18361 tatttaattc tttagctttg tcttctgaca tgactaacat catcgcagca ccatcattga
    18421 ttcctgatgc attacctgct gtaactgtac cgtctttttt gaaagctggt cttaatcgac
    18481 ttaatttttc gactgataca ttttcacgta caccttcatc cttagtgact acgattggtt
    18541 cacctttacg ttgaggaatc gatactggaa ctatttcact atcaaattca ccattttgct
    18601 gtgcacgtac tgctttttgt tgtgagttta cagcaaatgt atcttgttct tctcttgaaa
    18661 taccatattg ctctactaaa ttttcagcag taatacccat atgatattga ttaaatacat
    18721 ctgttaaacc atcatatacc atgctatcaa ccattgattg atgtcccatt ttaaaaccaa
    18781 agcgactgtt gttgacaagc attggtgatt gagacatatt ctccataccg ccagctagca
    18841 cgatgtcatt ttcaccagtc acaatagatt gatatgctaa ttgaatcgac tttaacccag
    18901 aaccacatac tttattcacc gtaaatgcag gtactgtttc tggcaagcca cctttcatag
    18961 cagcaattcg tgctggattt tgtccttgtc ctgcttgtag tacgttaccg atgataactt
    19021 catcaatctc acttggattc aaacccgtct ctttaataat atgttctatt aaagtcgcac
    19081 ctaaatcata ggctggcacg tctttaaacg cacctccaaa aacgccaata ggtgtcctgt
    19141 atgctgctgc taatacgact ctcgtcatgt catattcatc tccttatgat atatacaatt
    19201 ttattactaa tacgtctaat gaaccaatac gccactaaca tccaatttat gaaaccaatt
    19261 gctacgtatg ttacattttc attcttgtta tgcatttaac aaatcatatt atacaatgat
    19321 gtaatttgtt tgggcaatcg gaaacgtaaa cggtttcact ttaacatttc accctcttaa
    19381 atatgctcta tcatcctcat ctatagtaca aaattatgct ttgatttacc acaaattatc
    19441 attaatcatt tcgcgtaatg actctaatta aattctatct atcattgcat cgctattcac
    19501 agctggtttc accatcaatt tctcatcaat tttttattac catgacgtct ccagtcaaat
    19561 attaaatcgc tgatgattgt taattccgca attaccttgc agtcacctct ccttccactt
    19621 catcgccaaa tctgccccta ttcactagaa aaaataaata gtatacttag taaattaatt
    19681 acacatctta aaggagttaa aattttatgg ttaatgcatt aacaccaaaa catttagctg
    19741 aaaagcgtgc aggattccat gagttgttct ttgatttaat tttcgtctat gccattcaaa
    19801 aaattgcaca tgtgatttta acaacacaaa acggttccat ttcagctgat ttatttttca
    19861 aatacatcgt catgagtttg ttcttatggt taatgtggtc tcatcaaaca ttttttacaa
    19921 atcgatttgg tcaagttaca ttcaaagacg tctcgtttat gatgtttaat atgttcatca
    19981 tggtcttcct atctaacagc ctatatccgg actttgaaaa gacattcttc ccattcttct
    20041 tatgcgtggc aattatgtat ttaagtatcg gattacagta tttattacat atacgtacag
    20101 ggttagatta tggcgataag cgtacatgtc aagcttttgc gagcgtagcc tttgtgattt
    20161 catttttatc atttctatca ttagtactac cacaaagtat ccattacatc cctggatttt
    20221 taggcgtatt cattgcagcg acaggactta ttccatttca gaaatatctt gtattatcac
    20281 ctgttaatat gatgcattta gtagaacgtt tttcgttact aacaattatt atctttggcg
    20341 aggtactggt tggtttagct tcgtcatcat ttagcattga tcattttagt tacatttata
    20401 tattccaatt tatgatttta attagtcttt tcggtgtata ttggatcatt actgaaaatt
    20461 atattaatca taaattaaca tctatcggtt tccgtttatc gtatacgcat ctattaatca
    20521 atatcgcttt aggtgtgatt aatgctgcaa ttgtttttag taataacaat aaactaaatg
    20581 acttgtttga aattaatatg atgtatatca gtgttttaat attctatatt ggattatggt
    20641 taatcacgcc ttatttccat aatgaactga caaacgcgaa gtatatttca tattcactag
    20701 gtatactcgt tatgtcttat attgtcagcc ttttatttaa aggtcacgac catgtaatga
    20761 ttatctcagt atcagttgca accttctgca tcatgctgat ttattttaaa aatcaacgtc
    20821 tacgtaataa acatgaataa atagtattta cctcggtcaa agtatttgaa ctgaatccaa
    20881 aagtgtcgaa cttcacatta tacttttgtg acaaacttca gttatgctac atgatcgagg
    20941 tttttaaata tttccgagga aatcgttagc ctatacaatc atcaaatgat acttataaat
    21001 aacatatatc ctctaatccc cactatctaa aaggaaataa ttcccacaca acataaccac
    21061 ctcaaaacaa ataaatcaat atagatatgt cacctatact tatcccccac atttcattcc
    21121 acaccaaaaa gaacagatga caatcgtaaa gatttatcac ctgctctaaa tattatttca
    21181 aatactatca tcaccaaaat cgacaattat agttcttgtt tgttaaaaat tttaatagac
    21241 aatatataag aaacgctcgt tataactata ccaatgaccg catatgctat gacaactagc
    21301 catccttcag tgctaatttt ttcaaattct aatgcagatg caccactaac cataccatat
    21361 ataaagaaaa agaccacgaa agaaataagt agtagtatag atgcacatat cataatgaca
    21421 ttggagtttt cagcgccaaa tttaaatgtc aatgggaaaa tgatagagta tgcccctatg
    21481 ataccaaaac tcattaaacc tgacataata ccaatcatca cactttgggt cacaatcgta
    21541 gtcacaacta atccaatcat taaacttgca ccgaataaga ttaaataaaa ggcaaagtat
    21601 gacttaatat aatcactacg tttaaccggt aaagtagata cataatacat ccatcttgag
    21661 tctttttcat gtttaatatt atcagtaata ggtgtgatta acataacccc agccattgcc
    21721 gaactcatca acggattaaa tactgcaaag tatcctgcag ctataatagc gactataaaa
    21781 taaatatatg tttgctttct cgttgcataa aaactactta ggaacatacc tttcattata
    21841 cttcacctcg cattatgatt tttgtagcgt catcaatgtg ttttaaaggc tctgcatttt
    21901 caatttctgc ataatcttta actaaaattt gatatttccc ctttgacaat ctagaagcaa
    21961 tgattaaatg cttaggaatt tcaacatctt tatcttctgt cgtaacaatt ccatattgtg
    22021 ctaaaagtat atctttctgt tcagttaaaa tcattcgtcc atctttcata aacactaatt
    22081 tatcagctaa atgttctata tcttcagaaa tatgcgatga tattaagatg cctccacctt
    22141 gtgcgacaaa atcttctagt atttccatta cttcttctct tccagaaaca tccatacctg
    22201 cagttgcttc atctaagatt aataacttca catcatgaga aagcgcaatc gttaaagcta
    22261 tcttcattcg catccctctt gaaaaagttt taattttagt ttgtagtggt aactcgaaat
    22321 atttgattaa atcaaagaat ttttgactat tccaagtcat ataaatagat tgaaatactt
    22381 tatcaatatc tttaatagtc aatttattag gcactctcaa atcatcaaac actacaccta
    22441 tgtgttcttt atattcgaca tcatccacgg tatgttcttc tccaaaaaat gtgatactac
    22501 cgttatcttt atgtcgattg cctactagaa tattaattaa tgtcgattta cctgagccat
    22561 ttttcccaat taatccaact gtctcattag tagaaatcgt taaagatgca tcttgcaatt
    22621 taaaatccga atctttgtat tgtttgttca cactatctac gtttaacaat gttgtcatat
    22681 ccatgctcct ctttgtttaa ttttaataaa aacgtcttct cttcaataaa taactaaagg
    22741 cgttatatat gaaaatagca atgataagta gcaaactaaa gctttgatta attccagtag
    22801 taattgaata aagcccaatt actacaatgg ctagtatcaa tattgataag ttgatcgcat
    22861 atgttttaaa taatgcaatt aattctatat ggcgttcacc ttcatccaat atttccaatc
    22921 gcttttcagt gtaattctta tctgcaattt ttggtattct gtcatcaaac cttctattaa
    22981 acagtgtaaa ttgtgtattg aaaatagcac tagcaaaaaa tggtattaaa aagaatagta
    23041 ttgcatttgc tgcagcgtgc cctaccacaa aaataagcaa tactaaaaaa gcaataactg
    23101 tctgcagaat acttaatata cttccattta aaacataacg attcgcaagc aactcatatt
    23161 gatctgttgc atcgatatct acctctttat ctacaagttg cttatacttc aatgcacgtc
    23221 tttgattcat caaaacaatg gcttctacca atatgataat aatcgtcgca acaatcgata
    23281 ttacaacgac attgttatat gtcgcaaacg tcaagttctc aataccaaat ccatcaaaat
    23341 tacctaaaat tccacctatg atacccccta caagtccacc cagtaaaaga tatccgatat
    23401 atcttagtat tttcatgcct catcctcctc aataataaaa acagtttcca ccgtttcatt
    23461 gaaaatgcga gcaattttta ttgccgttaa tactgatggc ataaaattgt ttcgctcaat
    23521 tagcgatatg gtttgtcttg aaacgcccgc ttgtttagca agttgcgttt ggtttaagcc
    23581 atctcgtgct cgtaattctt tcaatcgatt acgcacatcg catcaactcc ttaattacac
    23641 tgtaaatgat atgcgtcttt ttgacaacta tatttgtcaa atctacacaa aaaaatatga
    23701 ttatccacct atgtatgaca ttttgaaaca aacacctcaa cgcctacaag tcataattgt
    23761 ttactttcgt tacaccttcc tgcataatta acagcattct aattttagta tgatgcacgc
    23821 attttcacta aatcaaacca ttcaaaggag actattatgg catttacatt atctgcaatt
    23881 caacaagcac atcaacaatt tactggtgtt gactttccaa aactattcaa agcttttaaa
    23941 gatatgggga tgacttacaa tatcgtcaac attcaagatg gcactgcaac atacgtacat
    24001 caatcagaag atgatatcgt tacgtcatct gtaaaaagta atcatcctgt tgctcaaaaa
    24061 tcaaacaaaa caatagttca agacgtctta actagacatc aacaagggca aacagatttt
    24121 gaaacatttt gtgatgaaat ggctgaagct ggcatttata aatggcatat cgatattcaa
    24181 gcgggcactt gtacttatat cgacttgcaa gaccaagctg ttatttcaga attaatccct
    24241 caataaacta tatttatagc aacattttaa ttatttcata aaattttatt gataatcatt
    24301 atcgttcggt ataaagtaaa tactatatac tacttatgag tgaggttgat tatcatgata
    24361 actaacactt ttattttagg catcacaggc ccaacaagtc ttgtcgtcat tagcattatc
    24421 gctttaatta tttttggtcc gaaaaaatta ccacaatttg gccgtgccat cggttctact
    24481 ttaaaagaat ttaaatctgc aacagaagat ttagataaag agtctcacga tacacccagt
    24541 aaggaatcga aacaacagcg agagcaatag cactgaccac accttactgg ttcactttag
    24601 cgaactacgc catcggttag taaaaatttt attgtcgttc gtcattacgg tcatcgtcgt
    24661 atatgtttca tcattttggt ggatgacacc attcataacg tatattaccc gtgcacatgt
    24721 gtccttacat gcattttcat tcacagaaat gatacaaata tacgtgatga ttatattttt
    24781 catcgcgttt tgtttcattt caccagtcat gttttatcaa ttgtgggcat tcattgcacc
    24841 tggattacat aataatgaac gtcaatttat ttataaatat agttttttca gcgtgttatt
    24901 attctgtgct ggcgtcgctt ttgcatttta tgttggcttt ccaatgatca ttcaattcgc
    24961 gttaaaatta tcattaactt taaacatttc gcctgtcatt ggctttaaag catatttgat
    25021 tgaactcata cgttggttat tcacatttgg tcttctattt caattaccaa tattatttat
    25081 gggtcttgca aaattcggtc ttatagatac cacatcattg aaacattatc gtaaatatat
    25141 ttatttcgct tgctttgtat tagcaagtat cattgcacca cccgatttaa cattaaatat
    25201 attactcaca ttgccactca tattgctctt tgagttcagt atgttcatcg ttaagttcac
    25261 atgccgaggc aaaccgccaa cacattaacc ttatacgaat caatgctgtg attatcgtta
    25321 tactgcaata ccgaatattt ccaatatagc aacaaaaaaa acaggcactc ggcctgtttt
    25381 ttatttttta aatttaaaga ttaagatagc aactaccata atataagcac cttgtgctat
    25441 caatggttca taacttggat aaaagcccaa ccagttaatc gttgggaatc cttcaataac
    25501 atgtcttggc atcgcaccta ataattgtaa cttttgaata cttacgccaa gcattttgaa
    25561 tcccataata aaaataaaga tcgacaacac tctaaatata tagaaaatag gtattaattt
    25621 aactataaat ctaaataata atgcaaagat gattaaaata acgatagcta aagcaatacc
    25681 aataataaaa tctttggtcg ctagctcacc tatcatcccc atatagaaaa tgataacctc
    25741 gacaccttca cgcaacacag atattaaacc aatcgtcgct aacaatacca aattaccatt
    25801 actaatcgca ttagcataca tatttttaat catgtcattc caacgttttg catttgaacg
    25861 tttgtgcatc caaacaccaa cgataaacat taatatgacc gcaacgatac ctaatcccgc
    25921 ttccatactt tcacgaagaa tgccactatt ccctaaagtt tctacaaacg taattgctaa
    25981 gataatactc agtacaagtc cggcaattgc accaccaatc acacttgcag tccctttctt
    26041 atcttttaca ttacgcgtca tggtagtcaa tgtcattaca attaacaaca cttctagccc
    26101 ttcacgtaaa aagataatca tcacatcgac gaagctataa ctatggccaa caacctcttt
    26161 aatttggtta tttaaatcta ctaaaccatc tttcacatgt gctttattat gttcgtctaa
    26221 tacactttga taatatggta ttttatcttc aattttcgta tacaaagcac cgtctttagt
    26281 ttgaatttga ccttcaacat acggccaagt ttctataaaa tgtgtaagcg cagcatcagc
    26341 atccgacaat tgattgtcgt cgatagcttt aatcgccttc tctaacgcat catttaattg
    26401 tgatacatgg tattgatcat ttgcagacgt attacttttt ttatcgacat gatcaatatt
    26461 tgatttaaaa gttgtccaag catgtgacac ttttgccgta tctaatggtg acttatgaat
    26521 tgcaattcta agttgtaata atgcgacttc aatttgtcca tattgatttg cgtcataatt
    26581 gcgaatcact gtttcattac ttgtccaaat ctgattcaaa ctattgttca aagattctaa
    26641 ttccgcttta tttttatctt taatcgcttt tgtcatcgca gcatctttag catcgacttg
    26701 ctgttgcaat agtttaattt tagaacccgc atctttacta gccaatttct cttcataagc
    26761 aattaatgac ttcgttaatt gcgaaagtgt atctttttga ttatcattcg cttttgcatc
    26821 ttcaagcttt ctcacatctg atttgacagc attactttca ctattatctt caagcgataa
    26881 tttcttaact gcacttacca cttgctcaat tgctttctgc ttattgtcat tcgatatcga
    26941 attattagaa agtgcagatt tcgcatccgt tatcacacta tatacatcac taatactttg
    27001 ttgttctgct gcctgacttt tcagtaaccc aaagctacac accatagcag cagttattag
    27061 cattgctaca aatttagtca aataatgttt caccaaggta tcctccctta ctaacacctg
    27121 gtaatactaa aaatgaagca gaacctctat gtgtaatata ttcatttaat ttatcattac
    27181 tacctaaatt attttgtata tcgataaatt gttttgtcgc tttttgaaaa gcaataaaaa
    27241 gtaagcctgt ttcgaagtta cctgtgcggt catccgtacc atccacataa ttaaaggctc
    27301 tacgtaaaat tgacgtattt gcttctttcg ctagcctcgt atgggcatct ttatcaataa
    27361 tatactcgcc atgactatct ttcgctttta agtcaatttc atcaaactct ttcccacctg
    27421 ttaacggtgc accactatgt cgtttccgac caaatgtagc ctcttgttct tccagcgcag
    27481 tacgatccca cgtttcaatg tgtatttgaa tacgtctgac aacgcaataa gttccatgtt
    27541 tcgcccatcc gtcatcaata aacacataat ctttaagttg attattcttc ctaggattaa
    27601 ttgttccatc tttaaatgcc attaaatttc taggtgtttc cttaccttta gcagagataa
    27661 aaccagattg cgcccaacgt accttaacaa tatctcgaaa tggacgaact aaattatgaa
    27721 ccgcatgaaa ggatacttgc gaatcatttg agcatgcttg aatcataata tcaccatcgc
    27781 tgtaatcgtc tattaactga tcattcggaa aatgcggtaa atctttaaag gcatcgggaa
    27841 ttttactaga taacccaatt ttcttcatca aagacttact aatcccaaag gtaatcgtta
    27901 acttgcttgc acctaatcct atcgattcac cggtatctac tggtggcatt aaaggattgt
    27961 tactcggctt gccaactgta tcaccatcca tcatacgatc agccataacc gtccactttt
    28021 taaacattgc cttaattgca tctctatctt ttgacttcaa atctaacgca acgaaattgc
    28081 atgttttttg cgtgggtgtg gtaatgcctg gttgcacttt accataaaat tcatacgcat
    28141 ctttttccgg atcttctggt gtattgaaca ttgatttgaa agaccacatg ctaccaacac
    28201 cacttgcgcc aattgcaaca ccggcaccgc caatacctaa cattttcaaa aatgtacgtc
    28261 ttgaaaattg cgtactatcg ttaacttgtt cataatttgt cattcagtaa tcacagccat
    28321 tttacttagt ggctctccta atgcattaac agcatccgca aatgctttac ggtctttctt
    28381 cgatactttt tcaaatgact cataaccgcc gttgttatct ttatatttat ccaataattg
    28441 attcacttta tcgaagttca tttgaatatc atcacttaat tttttatctt ttttctctaa
    28501 aataggttta aataagtcat aaattttttg tgcgccttca acgttcgctt taaaatcata
    28561 taaatctgta tgtgaataaa tttcttcttc acctgtgatt ttagaagttg caacttcatt
    28621 taataggtca acagaacctt gtaacattaa ttttggtgtg atatctaatg tatcagcttt
    28681 ggcatgcaat tcttttgcat ctttcaataa ttgttgtgca tcttttttag tcacatcatc
    28741 aattttctta tcttcgtata atgctttttc aatcttatga tatcctgacc attctttttc
    28801 ctttttctct tctttcatat ctgcaagacg tgcatcaatt ttaggatcta aatctccaaa
    28861 tgcttctgca actggttcag agcgttcata atacatacga acttttggat ataacgcttt
    28921 tgcttgggcc atatcattat tttcaatcgc tttaacaaat ttttctgtac cttttaaaaa
    28981 ttcatttaac tgttcatcag tatatttatc atatgctttt gttgcttgtt ttaattctgc
    29041 tttaacacca tcatcttttt tcgatgtctt tgaatcatcc ttcttactat cgtcgttccc
    29101 acatgcagca attaataacg ttgatgctaa taatagcgtt gttaactttt tcattttgac
    29161 acctcattat agaaattcta ttatccccat agatgtgttc ttataaatat tattttcgca
    29221 ttccctacac gctaaaataa tacattacac ttaaaacggc tgttttaaag catcctccca
    29281 taaacatcat ctagttgata ataattatca aaatcattat attgctataa aataatactg
    29341 tcaacaacct tttattttca aaaattaaat aaaggagcgc tgccatttta aatgtcatca
    29401 ctcttttgta agtctaattt gtcatttttg gtcgtattct gattttaata aactatagat
    29461 gtaactcgat gaataaatac catttagtaa ttcattgtca cgtaacatac cttctctagt
    29521 aaatcccagc ctttcaggaa tagcccggct tttttcatta ttaactgcgg cactaatctc
    29581 aattcggttt aagtcaagct cttcgaaaca atactttatc aatgcctcaa ctgcttgtgt
    29641 cataatccca tgaccctcaa attctttatc taaatagtac cctaatgaag tttttctgtg
    29701 catgtgatta atttcatgta aaccgatgac accaactagc gttccttcat accaaatgcc
    29761 acactgaaat ccattaccat cagcaaattg caaaagtcct cttttaataa atgcacgcgt
    29821 atctgatggt tgctcagttg catctaccca aggtaaccat tccctaagtg aatttcttga
    29881 acgattgact aaattgaaaa gcgcttctgt gtcatgagct tctaaaattt ttaatgttat
    29941 ttgttcattc actttcattc caaacatacc atcacatcct cattcatttt tcatataatt
    30001 ctgtaaatta tcattataat aacatattaa tgttacgcat tgtatcttaa atttgttgtc
    30061 cttccccaac ttgcattgct tgtagaattt cttttcgaaa ttctctatgt tggggcccat
    30121 ccccaacttg cacattattg taagctgact tttcgtcagc ttctttgttg gggccccgcc
    30181 aataagcgtt gtagtgccta ttactttgat tgatgtccaa gtttcctatc acacaactta
    30241 atttaatcac tactttatta ttcattccaa atttatattt aaaaatacga caaaacagtg
    30301 ctaaaatgct attaaaggag gcactgctca tggcatcatt gaaaaataag cattttatga
    30361 ttgggctatc actaacattt atagtcgcat tatttagctt tttagcagct aaattgccaa
    30421 tcctagataa ggttggcgca ttaactatcg cgatattaat cgctattcta tatcgtcact
    30481 ttagaggata tcctgaacaa tatagctcag gtattacgtt ttcatctaaa tatttattaa
    30541 gatttgcaat catcctatat ggacttaaac tcaatatatt cgatattatc ggtcaaggta
    30601 gtaaactatt agccatcgat gtaggtgtcg ttattttcag tattgtaatg atgctctttg
    30661 tcaacaaact gttgcatggt gacaaaaaca tcgcattatt actaggtgtc ggcacaggcg
    30721 tttgtggtgc tgctgctatt gccgcagtcg ctccaatatt caaatcacgt gaaaaggata
    30781 cagctattag tatcggtatc atcgcattga ttggtacgat attttcactt atatatacag
    30841 ctatctacgc tatcttttca atgacgacaa atgtttatgg cgcttggtct ggtgttagtc
    30901 ttcatgaaat tgcacacgtt gtcttagctg gtggctttgg tggtagtgat gcacttaaaa
    30961 ttgcacttct tggtaaactt ggtagagtat tcttactgat tccattaacc atcgtactta
    31021 ttttaattat gcgtttccgt tcatcagaat catctagcaa gggtcgaata agcattccat
    31081 actttttaat tggatttgtc attatggcct tagtgaatac atatgtaact attccgtcag
    31141 cattgctaaa tattttaaat actgtatcaa caatttgctt attaatggcc atggttgctt
    31201 tagggttaaa tgttgcattt aaagatctta aaaatcgcgc acttaaaccg cttatgacaa
    31261 tcattattac ctcaatatgc ttgtcatcac ttgcctttat cgtcgttcat tggctatata
    31321 gttaatatga aaagacgctc aaacacttac aaatttagtg tgccgagcgt cttattttta
    31381 tactttaaaa gtcgtattgg ttattttctt cttcaaacac ttgttgttgt actttggcag
    31441 ttcgcattgt ggattgtgcc aacgctttta accgaaatac aacatcatca ttgacaattt
    31501 gttgatttga aaaatctttc tcttcaataa atacatacgt ttgcatcgta tgtgctttca
    31561 tgtaactcaa tattggtttt aaatgcattt caggaattaa ataatgttta ctagaacctg
    31621 ctgtcgctac aagtcctatt accttgtcac gaaacgcatt gactggaagt agatcaaaca
    31681 catttttcaa agcaccaggg atggaagctt gaaaaattgg aaaaccaata aaaatcacat
    31741 cagcctgcat taacgacgtc gttaatttat atacatctcc tgtagtatct agataattct
    31801 ttccaacgct aaattctaat tcaagttctc gtaaatccat caactctatt tgatgtccct
    31861 cattgatgac ttctagttca ttttttaaat catccatagc aattctcgtt ttagaaccta
    31921 ctgtggaacc tgacaataat acaatatcca tgatgacgcc cctcctattt tgataaatgc
    31981 tttttaatcg ccggtataat gtcgttgcca attaactcaa tattcttcat aacattttca
    32041 aatggcacac cgccaaaatc aagctgtgcc ataaaacgtt gatgaccata caactcgtgt
    32101 tgatacaata tcttttcaat aatttgttgc gggcttccaa ccattagcgc ttctcgataa
    32161 tctgacgaat tagcaaattg ctgtttcgga taaccaacac cacgaataaa tgacatccct
    32221 gtattcaaat gtggataaaa ttctctcata gcatcctgag ttgtttcagc tgtataaaac
    32281 agactcgctg tacttactgg taaagactta ggcgaagcat cgaaacctgc ttcagttgcc
    32341 gcttgacgat aagcatctat agaaccttta aagttcattg ctgggccacc aagggttgta
    32401 atcatcattg gcacaccttg ttttcccgct ttaatagcac ttgcaggtgg accaccaaca
    32461 gcacgccata ttggcaatat attatcgatt ggtcttggga atattttcat atttctaagt
    32521 tctggacgat attttccaga ccaagtaata cgctcagttt tatttaactc taaaagtaaa
    32581 cctaattttt cttcaaacaa ttcatcatag tcttttaaat catagccaaa caagtcaaaa
    32641 atacctgttc ttgatgatct gccagctaca atttcggctc taccatgaga aatcaaatct
    32701 aatgtcgcga agtcttcaaa tactcttaca ggatctgttg cactaataat cgttgaagaa
    32761 cttgaaactt taatatgctt tgtcgcttgg gcaattgcac ctaacacaac cgtatgtgcc
    32821 tgtgttgtaa aatgctcctg atgactttca ccaactgcaa aaacatcaat acctgcttca
    32881 tctgctaatt tacttgcttc aataatttca ttaatacgtt gctcataact aactttttca
    32941 cctttcaatg gattcaataa atgatcacct aaggaataca aaccaaactc aagaggcgta
    33001 tttttattca tttctaattt ggccattaaa cattctcctt tatatttcgt gtaacttact
    33061 ttcaatttgt tcacgttctg cttctaaaaa gtcaggcaag ttcaattgac ttcctaattg
    33121 ttcaacaggt gtatcaatag taaatccagg cgcttcagtc gcaaattcat acataattga
    33181 attatggcga tagtataatg atttaaagaa atagcgatca attatacctg aattactttg
    33241 tggttgttgt tgtaatttct tgtaaattgc atctaagtca ctcatttgtg gtgtattaac
    33301 tgcaatatga tggatataac ctcgtccagg tctttcacgt tgtccctgtt gctcaataac
    33361 tacaaaatcc gaatataaac cttgtggtgc taatgtcagc acatcgaatg atttattatc
    33421 tcttttgcga taacctaaga cattttccaa aaattctacc gtacgcgctg catttctaac
    33481 tcttaattcg actggcccta ttcctaaaat ttgatatgct tgaggtattt cactataagc
    33541 gttatgttgc cattgatgtg gtacttcaaa actatcatta acaagcaata tgatttctaa
    33601 gtcgtcttca tctttaaaga caacaccttc ttgtccaaga tagtacaaac gttcagactt
    33661 aataccatta ttctcaagac gtttttcaaa ataatgaagt gccgcttgat ttggtactaa
    33721 taatgaaaat cgataaatcg tttcagtacc tggctgctta tgacccgcat tgggaatttc
    33781 aaaaaagctt aaaattgttc cggctgtacc tacttcgtcc ccataaaaca aatgatacat
    33841 tgaaggattg tcttgattaa ccgacttttc aactaatcgt aatccaagga catttgtata
    33901 aaaatcctta ttacgttttg catcttttgt atacatggat atgtgatgat gccctactat
    33961 attcatacta tccctcctat tagtataaat tttataccag cttacataaa aaagatagtt
    34021 atatgccatt taccccttgc aaataaaaat atgtttgtac tcattcgtag atacacagct
    34081 agtatgtata acgacataat aattgcataa ttataaattt atgtgtagca atttattttt
    34141 tctaaatgtt tataaaatac ttcaataact aaatatgcaa gatttcacaa aagcatattg
    34201 acaacgcttt catacatgtc taatataagt ttatcgcaca aaatgcatat aaatacatat
    34261 taaggaggat tttatgaatt ttcttaaacc tgcaaagcat attaagcctt tgccagaaaa
    34321 tcagatagat gatacctata aacgattacg tctccaagta tttcttggta ttttcatcgg
    34381 ttacgctggg tactatttat tacgtaaaaa cttttcgtta gcgatgccgg cattgcaaga
    34441 gcaaggtttt acaaaagcgg aactaggttt tgcactttct gctgtttcca tcgcatatgg
    34501 atttagtaag ttctttatgg gtactgtaag tgatcggagc aatgctcgga tattcttagt
    34561 tcttggatta gcactcactg ctatcgtcaa tttgttaatg ggatttgtac cgttctttac
    34621 atcaggtatc ggtattatgt ttgtcctatt attcttaaat ggatggtttc aaggtatggg
    34681 ctggccacct tcaggccgtg ttctcgttca ctggtttagt gtaagtgaac gcggaagtaa
    34741 gactgccctt tggaacgttg cgcataatgt tggtggaggt attatggcac ctattgctgc
    34801 ttggggtatt acaacaactg catttatcaa ctttggttat ttaaaaggtt tcgaaggtgt
    34861 attcatttac cctgcactct tagcacttat cattgccgca atttcatacg tattgattag
    34921 agacacacct caatctcaag gtttacctcc aatcgaaatt tataaaaatg actttgctac
    34981 aagcgataag aaaacattag aaacagaatt aactacaaaa gaaattttat ttaaatatgt
    35041 actgaacaat aaatgggtat gggcaattgc ctttgcaaat atatttgttt atttcgtgcg
    35101 ttatggtgta cttgattggg cgccagtcta cttaagtgaa gaaaaacatt tcgacttaaa
    35161 agcatcaggt tgggcatact tcttatacga atgggctgga attcctggta cattattatg
    35221 tggttacatt tctgataaat tattcaaagg tcgtcgtgga cctgcaggtt tcttctttat
    35281 gttaggtgtc acagtatttg tattaattta ttggttaaat cctccaggca atgcttggtt
    35341 agacaatgtc tcattaattg ccattggttt cttaatatat ggaccagtta tgttaattgg
    35401 tttacaagca ttagattatg tacctaaaaa agcagctggc acagcagctg gattaacagg
    35461 attatttggt tatctgtttg gtgctgtaat ggccaacatc gtcttaggtg ctgtagttga
    35521 taaattcgga tgggatgtcg gttttatttt attaacagca attagtgtgt ttgcaatgtt
    35581 gagctttatc ctcacttgga ataaagtagg acaagaaacc gttcatcatt aaatgataaa
    35641 aaataaagtc atatggttat cttatcgaaa gatgatatat tcatctctta taagttcaac
    35701 catatgactt tttattagta ttcaaaaaaa tatttacatt gccacttttg tgtttgccct
    35761 gctgttttat tcaattgatt acaccactta ggataaactc taaaagccat tttcccttga
    35821 tacttagatg aagctaagat acctttttct accaataatt ccctaggaaa tagaaagtag
    35881 ccatttaatt catcatcgat aacagcaaca atcaggtaat cagcaaactc ttcaattttg
    35941 tatggccgat tataattatc ttcgtctttt gtccaacatg tcacgaaata tcccgattta
    36001 gtcggtgttt tcttagctaa cctactttga tatgtttcct ctttaaagct aaatgttaat
    36061 gcttcgtaat cttgattata tttttcttca gttaaatctt taacttctga ttcttcacta
    36121 aaaatatttt tcaacagtat tttagattta cacatatgcc aatctcactt tattttttca
    36181 taatcgtatc atatatttat ttttttcgaa aaatacactt ccgaccgaaa tatactcagc
    36241 cagaagtgga cgaaatattt aaccttctat taattcagat gtatcaactg tcaaacgatc
    36301 acgtaataaa tagacgatta acattgctgc taaagcacaa agtgactcag caattaataa
    36361 tgaccaaatg acacctgtta gtccaaacaa agcattcata ataaataata ctggaatgat
    36421 aattgcacct tgtaaaatgg ccataattgt agcaccacga ccttgcccag tcgcttgaag
    36481 cataccagta aacaagaaac ctataccatt taataataat gatgccattg ttactttcaa
    36541 aataaatgtc gccatctcaa caatggcttg atcagtagta aatagtccga ccatatgatg
    36601 tccaattgta aatacagcac tcatacatac aacaaagata acgccgatag acatgataac
    36661 tgctttgata acgtctttca tacggccttt atttgccata aagttatatg caattagtgg
    36721 tacaacacct tcacataatc ccatgataat aagttctgga aattgcacaa gtctaaatga
    36781 gataccataa cttgcaatcg cgaagtttcc ataatgtgct aaaaataaat ttaaaactaa
    36841 tcctgtgaat cccattaaga tactcattaa aaatgcagga ataccgattt taaagatttc
    36901 agaaagcatt tctttattag gtttcgcaag tttaatatta actgacacaa cgtcactatt
    36961 tttcataaaa taaatgataa agaacagagc agcagcaaca ttactgattg cagtacccaa
    37021 agctgcacca acaacgttta aatcaaaacc aaaaattaaa attggatcta aaataatatt
    37081 taagcctaca ctagctaaca taccaatcat agaaaccatt ggtgccccaa ttgcacgtgc
    37141 aaattgttct aatatgaaga acaaaattac aaaaggtgca cttaaaaaca ttactttcaa
    37201 ataattactt gttaaagcta acgtttcacc tctcgcccct aaaattgctg cgatttgatc
    37261 actgaatggt aaagtaacta aaatcacgat aagtcctagt gcaataccac cataaataga
    37321 gaaactactt acaaatttac tcttactata gtctttcgca cctaataaac gtgaaatata
    37381 agttcctgca ccaacgccaa ataaattacc taaccccatt aagatagcaa atactggcag
    37441 tgttagagag atagcagaaa tcatgtggct atcttctaaa aatcctataa agtaaatatt
    37501 taatatgcca taaataacgc ttaataaagt ccctatcatc attggcaatg agaaatgcat
    37561 catcgcttta aatactggcg atttctcaaa ataatataat tgttcgtctt tcattgttca
    37621 atactccttg tcttttccaa taattagctt actaacaatt agatatctaa ctataatatt
    37681 aaagacaaag tgactgattt ctaccagtca cacttatcat ttattgtaaa ctagataaca
    37741 ttttagttaa gtttgctttc atttgttcat tttcttcttc agataactgc gatacgagtg
    37801 tttgttccat ttcatcaaat atcgaagtga atgcttctac gagtttaatc ccagaggtag
    37861 tcagccctat attctttctt ctcgtatctt gtgcatcgac atagcgatag atcagctttt
    37921 tacgttcaag gttccttaat aaattactga cagttggacc tgttcgttgt aatgctttag
    37981 caatatcatt ttgtgtcagt ccatcttgtt gatgtgcata aagataacct aacgtatgac
    38041 cttgctcatt tgtaatatca aattgctcta acttttgatc agccttttgt ttcatctcat
    38101 gactaatcat tctaaataaa tacgaataag tgaattccat ttattgcacc accatttcgt
    38161 tagatgtcta actaaatata ctgcgacatt tttaaattgt caacatgaat atctaattta
    38221 ttttaaaagt tcctataaaa tcttgcgcca taaacatttt gatatgcaat ttttcggatt
    38281 aaaatatgaa agagattgca tatctacgtt tttaagaaat cgcttacaat tacaaatagg
    38341 aggatattac gttgttaagt tatagacaaa tcgaaatttt gtatctttta attcatgagc
    38401 aaacgtttat tccaatcaat accattgcta atcaacttgg tgtatcacct cgtaccattc
    38461 aatatgatat agcatatatt gagcaatatg ctgaaactta tcattaccaa gtcagccgta
    38521 ataaagctgc aggcatcaaa gtaacaacag ctcacgcgac actattaaat gaattagagc
    38581 acaatctaac aaatcaaatt catttttcaa aagatgaacg actcacacat atcgctttaa
    38641 agttattcga aacaaccgat cctgtttcaa caaagcaact tgcgcaagat gttaatgttt
    38701 cgcgtcggac aattgcagat gatattaaaa tgattcaagc ccaactagat caataccatt
    38761 taaagttaaa ttatgtccac aataaaggat tcaatattat tggtgaggaa gatcattatc
    38821 gtaaagcgta tgcacacttt atacatcaat atatgaaaca agctgcacct tttatagagg
    38881 cggatatctt taattcagaa tcaatcgcat tggttcgccg tgccattatt aagacattaa
    38941 atagtgaaaa ttatcattta gttcagtcgg ctatcgatgg cttaatctat catatactca
    39001 ttgccattca gcgtttaaat gaaaattttt cgttcgatat acctatcaat gaaattgata
    39061 aatggcgaca tactaatcag tatgctattg cttcaaaaat gatagaaaac ttagaacgca
    39121 gttgtaatgt cacatttcca gagtctgaaa ttatctttat cacgttacat ttactaggct
    39181 ctaaaatgac tgaacataca gcatcttcaa ttacctttga ataccatgat ttatcgcaaa
    39241 atatacatga attgatcact tgtgttagcc aagaattagg cattgatatg tcaaaagaca
    39301 acaagttaca taccagtctg atcacacata tcaaaccagc tatacatcgt attaaatacg
    39361 atatgctaca acctaatcct ttgaggcaag aagttatgcg tcgctatcct caaatcattg
    39421 aagccgttag caagcatatt agtccaattg aacaagatgc tgctattcgc ttcaacgaag
    39481 atgaattaac atacattaca attcacttcg catcaagtat agagcgtgtt gcaacacata
    39541 aacaatcaat gattaaggtt gtcttactat gtggttctgg tataggcacg tcacaacttt
    39601 taaaatcaaa actaaatcac ctgtatcctg agtttcacat ttgggatgcc tattccattt
    39661 atcaattgga agaaagtcga ttattgcaag ataacattga ttatgtcatt tcaacagtac
    39721 cttgtgaaat atcagctgta ccagttattc atgtcgatcc atttatcaat caacaatctc
    39781 gtcaaaaatt gaatcaaatt atcaatgact caagagaaca acgagtcatg aaaatggcaa
    39841 ctgatggcaa gtcactcgca gatttattgc ctgaacatcg catcattata aataaacaac
    39901 cattatcaat tgaatccgca attgcagtgg ctgtgcaacc tttaatcaat gatggcattg
    39961 tctattcaaa ttatacagct gcaattttaa aacaatttga acaattcggg tcatatatgg
    40021 tcattagtcc acatattgca cttattcacg ctggtactga ttatgtacag aatggtgtag
    40081 gtttcgcact aacatatttc actgaaggga ttatctttgg tagtaaagct aacgatcccg
    40141 ttcaccttgt aattacatta gcaacggacc accccaatgc acatttaaag gcattgggac
    40201 agttaagcga atgcttaagc aacgacttat atcgacaaga tttcttagat gggaatattt
    40261 ttaaaattaa acaacacatt gctttaacta tgacaaagga ggcttaataa cgtgtcatta
    40321 gacattttgt caacaacacg catcattgta aaagaacaag taaatgattg gactgaagct
    40381 ataactatag cttctcagcc attactacaa gaacaaatta ttgaacaagg ctatgttcaa
    40441 gcaatgattg atagcgttaa tgaacttgga ccttatatcg ttatcgcacc tgaaattgca
    40501 attgcacatg caagaccgaa caatgacgta catcaagttg gtttaagtct attaaagttg
    40561 aatcaacatg tggcattttg tgatgaagat cactacgcat ctctcatttt tgtattgagt
    40621 gccatcgaca atcattcaca cttatctgta ttacaaaatt tagcaaccgt actgggcgat
    40681 aaccaaacag tccagcaact attaactgca acaaatgcac aagacattaa aaacatttta
    40741 aaggagcatg attaatatga aaattttagt agtatgtggc cacggtttag gaagtagttt
    40801 tatggtagaa atgaacgcac aagaagcact taggcaactt aatgcaccat ctgatatcga
    40861 agttgaacat agtgacatta tgacagcaag tccagagatg gctgacttgt ttatttgtgg
    40921 tagagattta gctgaaaatg ccgaacgtct aggggatgtc ttagttcttg ataatatttt
    40981 agataaagct gaattacaac aaaagctctc agaaaaatta caacaactta acatgattta
    41041 aaggaggtac gacctatgca agcaatcctt aattttatag tcgatatttt aagtcaacca
    41101 gccattcttg ttgcactgat tgcctttata ggtttaatcg ttcagaaaaa acctgccgca
    41161 acgatcactt caggaaccat taaaacgata ttaggcttct taattttaag tgcaggtgct
    41221 gatgtcgtcg ttcgatctct tgaaccattc ggcaaaatat tccaacacgc atttggtgtg
    41281 caaggtatcg tacctaacaa cgaagctatc gtctcactag ccttaaaaga ttttggaaca
    41341 acagctgcac tcatcatggt ctgtggcatg attgttaata ttttaattgc ccgcttcact
    41401 aatttaaaat atatcttttt aacaggtcat catacatttt acatggctgc gtttttagca
    41461 atcattttaa cagtcagtca tattaaaggc tggctaacga ttgttatcgg cgcactcgta
    41521 ttaggattaa tcatggcagt attacctgca ttactccaac ctacgatgcg aaaaattaca
    41581 gggaatgacc aagtagcttt aggtcatttt ggctcaatca gttactttgc cgcaggtgct
    41641 gtaggtcaat tattcaaagg taagtctaaa tcaacggaag agattaaatt tccaaaaggc
    41701 ttaagtttct tacgagaaag tacaattagt atctcgatta cgatggcatt actttacttc
    41761 atcgcatgct tatttgcggg cgttagttat gtacacgaat ctattagtga tggtcaaaac
    41821 tttattgtct tttcattaat tcaaggtgtg acatttgctg ctggtgtatt tattatttta
    41881 acgggcgttc gtttaatctt agctgaaatc gtcccagcat ttaaaggaat ttctgaaaag
    41941 cttgtaccaa attctaaacc tgcattagac tgccctattg tgttccctta tgcacaaaat
    42001 gcagtattaa ttggattctt tgtcagcttt attacaggtg tcatcggtat gtttatctta
    42061 ttcttatttg gtggcgtcgt cattttacct ggcgtagttg cacacttctt cttaggtgca
    42121 acggctgctg tattcggtaa tgcaagaggc ggtattaaag gtgctattgc tggcgccgct
    42181 ctaaatggta tcctaatcac gtttttacca ttattattct tgccattttt aggcgaatta
    42241 ggtggtgctg caacaacatt ctcagataca gactttttag ctgtcggtat cgtgttcggt
    42301 aacgcagtaa aatatatggg attatttggt gcgattctat ttattattat cgtaggtgcg
    42361 acaacaattt tattaaaagg ccgtcaaaaa gaacagcaat agtgttaacg tagaaatata
    42421 aaacaccgtc acatattgag tgaatgcccc ttttatcaag aggaaagcca cttacttatg
    42481 gacggtgttt tgtattatat taaatgatac ttagccatac tatcgacagc tgctaaaatt
    42541 gcttcttctt gtgtcgcaat cgcttcccaa ccaagtaatg tttttgcacg ttcgttactt
    42601 acattgcggt tcatatctaa taaaagttta ccttctttag cttgatgatt aaatttagca
    42661 cctagactca aaataaagtc tggtaatttt ttagtagaaa ctttttgagc tatttcaggt
    42721 ctcttttctt taattaattt tgcaatttcc aacaaattaa tttgtccatc agccgtcgca
    42781 ataaatcgct tgccattagc ttgttcattt gtcattgcca aaatgtgcag ttcagctacg
    42841 tctctcacat caacaacatt taacggaatt tgcggtacac gtttcattga accattcaat
    42901 aaattttcta ataaatgaaa gcttcctgaa acgtgtgcat ctaatgatgg cccaaaaatt
    42961 gcaactggat tgattgtggc aaattctact gttgtatttt cattctcaac aaaatcccac
    43021 gctgcctttt cagctaacaa ttttgatttt tcatatactg ataagcctgg ttcatcttca
    43081 tttgtccaat gactttcatt tgtgattgaa tttttatctt tattactaaa accaactgca
    43141 ccaaagtttg cagtcattac cacacgttta acacccgcat gttctgcagc tcttaaaata
    43201 cgttgtatac cttcaattgc aggcttcgcc atcacttctg catcgtctgt tttaccgaaa
    43261 aacaccggag atgctacact caagacatac ttgcaatctt tcattgcttc atcccaatgt
    43321 tcatcttgtg ataaatccgc ttcgacaaac attaatcgct ctgtggaaat gccattgtct
    43381 tgcattgttt taattacttt atcagcttta cttaaatcac gtatcgtcgt ttgtacgtca
    43441 taaccttgtt ctaataatcg tgaaataatt cgcatgccaa caaaccctgt accaccggtt
    43501 actaatactt tattattcat cacataatct cctctttgtg tctattgatt actcattata
    43561 gcagtcatta tattttgtat atatcaacag acttatattg cttaatattc tatatgaatt
    43621 ataaaagtaa acatcattat attccttaaa atattaaact catctatttt caaataccca
    43681 atcatttaga aacaaaaacc atatatatat catactttga atagttaaag gcaataattt
    43741 ccactcatat gttcaacctt caccaaacac tattgtcacc ttttcaagag caagcaaaca
    43801 cttccaatga ttcagcatac ataaaataac aatattaact taaaatcata tccactaatg
    43861 cttctaccgt aacattgcca aaataataaa cgatgtctaa ttgctttaac tgatgcgtta
    43921 aatcttctct tgtcattttt gttccttgta atgcttcttc cacatctttt atatcacctt
    43981 gtccaaagaa atccccataa atacgacaat ctgcgatacg attttgttca acagaaattg
    44041 ttatgtctac cgtacctgaa gataatcttt cacttcgatt gtattcatat ttgggtgact
    44101 tgccataatt ccaatcccaa tttttatact ttttatcagc taattcatca atcgcttccc
    44161 aatctgcatc cgttaattca tatcgtttcg cctctttaat gtcatcgatt cccaaaatct
    44221 gcgtcaccat taaattttta aattcttcaa tggttatatc acgatacttt tcatccagtg
    44281 cttctctaag atgaccaaca cgtgcgcgca cagatttaat ccctttcgat gcaatctttt
    44341 tgcgattagg ctttaacact ttatccattg cttcataatt aacatcaagt aatagcgaat
    44401 aaccgccata aatacgatta ttcattaatg tcattgcggc gcctgacact tttttaccat
    44461 tcaatgttaa atcatttcta ccgctttgta ccacatctgt tgcacctaat gtatgcaacg
    44521 cctttatagc tggttgataa aatcgttgaa aatcaccata aattgaagtg tcttgttcta
    44581 aaatacaaca catattaacg gcacctttat caacatacac agcgccacca ccagtatctc
    44641 ggcgaactac ttgaatactg tgtttatcta tataatcttg attcacttct atagcagtat
    44701 tttgaaaacg tccaatttcc acttttggat cacaataata agggaataaa atatcttcat
    44761 ctaaaaagat atgctggtta acataaactt gcatagcgag tgcaatcgca ccatcagtaa
    44821 tatattctcc atttctaatc ggttctatta agtacatctt ctgtctctcc aatgttgtta
    44881 tttttcgttg tgctgtcgtt ccggagtgct gctgtaatca atgttgagat atcctcagtt
    44941 aaatgtatgg tacgttcttg aattgaattc ggaatgcgat atgccttttt attcatcgtc
    45001 atataaaggg cattttcatt tttacgtgtc atacgctgaa aaggatgctt cacaaattgt
    45061 ggtgtagtat aaccaattcc aatttccaaa tacaacactt tatcatcttg atgttgctct
    45121 agaaaagcat tataacgatg tagttgcgca tgaaattcag catcttcaac catcccaact
    45181 tccgctttac gtttattcac ttccattggg gcatcacatt ttggacatct tggaatcatc
    45241 tcccaaggta taagcatatc ttgttgcgca acaaccattt tacgaattaa atcatcattg
    45301 cgatacgttt gagcatgaca atgctgacta cactgttgca gtatatactc cccttgtata
    45361 tgaaatacat gagtcatatc ataatcagct acatcgaaag cattatctgc attcgtagtt
    45421 ataatgtggt actgtttacc ttccaccaag gattttaaag cgaggtaaga ctgacctaca
    45481 ggttgatcta aatagtttaa tgtaataaaa cgactctcaa atgcccaata ctcttgccaa
    45541 ctgccataag gatgtaaact cgcttgcaac atatcaaaga agcgatattt ttcaataaaa
    45601 tctgggaaat tttccgtaaa acgctctcct acatatgtaa atccgtcaga tgcagacatg
    45661 cctgcaccaa ttccaatcac tatcgcatct gcttcatcaa tcgcagtacg caatacttca
    45721 gcctgctttg tcttgtcatc cattaacaga gacattgcat tccacttact actctgcatc
    45781 acggttaaat gcctccttat acagttgtaa atccttatct gtaaatacat tgaacacgac
    45841 tttcaatgtt gaatttgttt ctttgagata gctttctact gttcgaacag caatttctgc
    45901 tgcttcatct tgaggaaaag caaatacacc tgtagatata cagcaaaaag cgacatgatt
    45961 taaactatgt tgatcagcca atttaagaca gctaagataa catttagcta acaagtcctg
    46021 attcatcttt gaaacaggca atcgacgtat ttgcggacca accgtatgaa ttatatactt
    46081 tgctggcaaa ttatatccac gtgttatttt ggctttacct acaccttcat tgcgcccttg
    46141 ttgtcgaatg atctctgcac aatcaagtcg aacttgaaca cccgcttttg tatgaataat
    46201 attatcaatg cagtcatgat tagcttgcat acatcctaga aaacgactat ttgcagcatt
    46261 aacaatagca tcgattttta acgtcgtgat atcaccttgc caaacaaaga tattgtcacc
    46321 ttttatcggc ttcaaatcct tcaaatccgt aacatgttga cgattcaaat cacttaacat
    46381 agcatcttgt acagctaaat attcatcact tacaggtaat gccggtctga catttgctaa
    46441 gcctcgatat aattcccaca aatcttcaaa tgaagatggc attaccaata cgtcattgtc
    46501 atttctctct cgatgcatat cattgattaa atattcaagt ctcgctttat ttgattttaa
    46561 cgtttccatt taagcctccg gtaatgctag gaatgcttct ttatcgacat catccaattt
    46621 gaacaaccaa ttttcttctg gtttttcaga gtttaaaatt gtcggttctt cttccgcttt
    46681 tgtatttcgc tcaataatcg ttcctgacaa tggcgtttgc acatcaatga ccgttttcga
    46741 tgcttcgata ctcacaattt catcatccac tttaacttca tctggactta cgaattcaac
    46801 ataacctact gtcccaatat catcttgcaa ttcaggtgtc atactaaaca catacaaatc
    46861 tcctactttt tctacccata aataattggc taactttttc atcttatctt catcctttct
    46921 cttctattta aaatgctaca aataaatttc cgcgagtaat tttaatgttt tacaacgctg
    46981 ttcaatacct ggaataagcg gtgctactaa cacctcatca acttcaaacg tagcaatgaa
    47041 atcatctaat tgtgctttaa ccttttcttg tgtacctgca atgatgcgtg cttgatgtgc
    47101 ttgaatcatc tctttgtctc gatcattaag cttatacttt tgtgctgtgt ctactgaagg
    47161 aaaatcttca aattctgcaa attgtaattt acctaataac caaacatcta aggcatgttg
    47221 taatgctgct acttccgctt cgttatcagc tacaatgaca aatacagatg ccatcacctt
    47281 tgcgtccatt ttaatcgttg atgcttggaa atgttttttg taaatatcaa tgttatcctt
    47341 cgctgcatgt atcgcattta tatctggtag caaaaatgtt ccaacagaaa gccctatacc
    47401 tagttcggca gctattttgg cagatgttgc gctactactt aataaccaca tttctggaaa
    47461 atgatcaatg tgtggttgga cacctaacgt atgcgcactt ggtttatcct ttattgtaag
    47521 ataatcacgt aataacgaaa tcgattcatc gtaactatca tatgtaggat ttattccatc
    47581 taaagcttgc tttaccatag tagtacctgg attattacca atacctaaat caatacgatt
    47641 tggatataac gctgccatca ttctaaaatg ctcagcaatt ttataaggtc gatagtgcgg
    47701 cagcatcaca ccaccagagc caactcgtat gtgatttgtc tgcgccaatg tatgcatcat
    47761 caaaagttct ggactactac acgcaaacgc tggtacatta tgatgttccg taaaccaaat
    47821 tcgcttaaag ccaagtcgat ctgctaattt tgcaagtgtc actgaatctt gcaatgcctt
    47881 ttgtgcatcc ttaccttcat ctattaaggc atagtctaat acgcttaatt taaccaatcc
    47941 gtcatctcca aacttatcct gtcatgtcaa accgacataa cattttagcg ttcttaatac
    48001 cattccctct tcatataccc acgtatatga taacgttttt caattaactt ttatatcttt
    48061 tcgcctttat ttttcttttt catattcata ttttcagacc tacaaatgcc attcattagt
    48121 ttaattttaa aaatactttc catgtaccaa ttctattcta tcaagactgt tcatcattga
    48181 ttattcattc tttccatggt actttgaaaa caatattgaa tagaaaagga atcatagtat
    48241 gtaccgatac aaaccacttt tacaatcaat ccacttacct aatggcatca aaatttcgaa
    48301 tcgctttgta ttatcaccaa tgaccgtaaa cacatcaaca aaagaaggct atattacaaa
    48361 agaagacttg gcttatgctg cacgtcgctc taattcagct ggaatgcaag tcaccggcgc
    48421 tgcttacatt gaaccttatg gtcaattgtt tgaatatggc tttaatattg atcatgatgc
    48481 ttgtatccct ggattaacta acatggcatc tacgatgaaa caacatggta gcctcgctat
    48541 tatccagcta gcgcatgctg gtcgtttttc aaatcaagcc attttaaatt ttggaaaggt
    48601 gtatggacca agccctatga cattgcattc accgattgaa cacgatgtta tcgccatgtc
    48661 tcatgagaaa atcaatagca tcattcagca gtatcgcgat gctacattac gcgcgattaa
    48721 agcaggtttt gatggtgtgg aaatttcgat tgcacaacgt ttgctgattc aaacattttt
    48781 ctcaactttt tcgaataaac gtacagacca ttacggtgcc gactcattaa aaaatcgtgc
    48841 aagattatgt ttagaagtta tgcgtgcggt acaagaagtg attgataaag aagcacctga
    48901 caattttatt ttaggatttc gagcaacgcc agaagaaacc agaggcagtg atttaggata
    48961 taccattgac gagttcaacc aattaatcaa ttgggtcatg gacgtttcaa acatccaata
    49021 cttagcaatc gctagctggg gtcgacatat ttatcaaaat acgtctcgta caccaggtga
    49081 tcatttcggt cgaccagtta accaaattgt ctatgaacat ttagcaggtc gtatcccttt
    49141 aattgcaagt ggcgggatta attctccaga gtctgcttta gatgctttac aacatgcaga
    49201 tatggtcggc atgtcttcgc cttttgtcac agaacctgac tttgtgcata aacttgctga
    49261 acagcgacca catgatatca atcttgaatt ttctatggca gatttagaag atttagccat
    49321 tccacatgct gcttttaaag atattgttaa aatgatggat tatggggaag gtctaaagaa
    49381 gcatacccgt gacgctttac gtcaattgga acagaattat catgactcat cgtcttaaga
    49441 ctatttttca tggaaattta agacagtgtt ataatgacat caatatcaaa aataaggagc
    49501 ctattaaatc gtgactgaaa ttaaagataa agtaattact aaagatgcat ttgcattacc
    49561 ttacacaatt attaaagcaa aaaatcaacc aacaaaaggc gctattgtct acatccacgg
    49621 tggtggatta atgttcggta aagctaacga tttatcccca cagtatatcg atatccttac
    49681 ggaacattac gatttaattc aactaagtta tcgtttatta ccagaagtaa gtctcgattg
    49741 cattattgag gatgtctacg catcattcga tgcgattcaa tcacaatatt ctaattgccc
    49801 tatttttacc tttggcagat catcaggtgc atatttaagt ctattaattg cacgagacag
    49861 agatattgat ggtgttattg atttttatgg ttatagtcgc atcaacactg aaccttttaa
    49921 aacaactaat agttattacg caaaaatagc tcaaagcgta aacgaaacaa tgattgcaca
    49981 actcacttca ccaacaccgg ttgttcaaga tcaaatcgca caacgttttt taatctacgt
    50041 ttatgcacgt ggcacaggta aatggataaa tatgattaac attgctgatt atactaattc
    50101 aaaatataat attgcaccag atgaacttaa aacacttcca cctgttttca ttgcgcattg
    50161 taatggcgat tatgatgttc ccgttgagga aagcgaacat atcatgaatc atgttccaca
    50221 ttcaacattt gaacgcgtga ataaaaacga gcatgatttt gatcgtagac ctaatgacga
    50281 agcaatcact atttatcgta aagttgttga tttcttaaac gctataacaa tgatgtaata
    50341 tatcattatt ttaaaaaact caatttatca tcatgataaa cagttattcg atcaaccata
    50401 ttattcatac aatttattaa gctcataaac agatacgcct gtccctttag ttttcgctaa
    50461 tgagacaggc gctttatttc actttactat ttttaaattc aacactgtga tgctacttat
    50521 atctgatgtg ccatgataac agcacgattt acatagcata ttaaattcag aagatgaatt
    50581 taacttgctt tcaattgtgt tcctttactt tcagtcgctt caacacgcaa caagtcattt
    50641 ataatacctg tatagaagtt ggcaagttct gcacctttac gtttgaaatc caggaagtcc
    50701 acaccgataa aatcgacatg atcccatcct tgtatgattg gtttaacttg ccagatacct
    50761 ctgcgtgtgg caggttcatc attcgtaaca ttaacaaatg gttgattgga cggatgtaat
    50821 gacgaaatca ctggtacgac accatcattt ttacgccatt cttctcttgc atcatgacca
    50881 ataattctac tcgttgtagc cattaagaaa aatgtaccta aatcaggatt ttcataacct
    50941 aatggaccag tatgagatga tacacctgta taagtcgtat acgtaatatt aggattcata
    51001 cttgtcatgt tgttcaattt tgcagagcca tctaacgtta aatcataggc agcattgtcg
    51061 tctgatgtcc aaattttgct tttactaacg cgttttatat agtcaatgta actctcattt
    51121 ggtaattgtt taaagcccca ttgcgttaat cctaaatcga tattcgaata cttgttaccc
    51181 ataaatcgat ttaaagcgaa catgattttt ctaacagctt ctgtatttcc aaacttatca
    51241 gctgcttgtg aaccattatg tggtgttgct aatgttgtga ttgatgcaac catattgtta
    51301 tgaccaccag tgaataatgg tgatatttct ccaccatgcg ctttatgata ggcaatttct
    51361 tctttgttac catttcttaa aaactcttcc attaaacgaa ttgtttgacc acccatacta
    51421 tgccctacaa gatgtacctt tttaccaggt tcccaattag gcatgattcc tttataagtc
    51481 ttaccatagc gctcatgtcc gtatttagct gcatgtgctg cgccataatc tacgcgacca
    51541 cctttaatgt aataataaag ttctacagcg cgatcatagt tactaccaaa tgcacttaca
    51601 cttgcttgat gtacattata gccttgcttt ctcaattctt cgataacttt aaatttattt
    51661 ccaccccaat aatttggata taaagcaggt gcattatcgc ctactaatcc taaaaatcca
    51721 tgtacaaaaa caactggata tttgttaagt ggttgtactt gattcgcttt aagtggtcga
    51781 acctttttag catctgctgc tgcatttgta gtcgaacgtg ttttaggcgc ttgaagcgca
    51841 tctttgatta gttgtttatt atcagtattt ttatctgtcg gtttctctgg ggaattttga
    51901 ccatcgatat gtttacttaa atcgccaact tgtggtttct gttcactttg gcgaacagta
    51961 tcatcttgct ttgcatctat gatttgatgc gcaggttgat gtgtatcttg ttgatttgga
    52021 tgtttgtccg tggttgcatc ttgtgctttt gttgatttag gtgcagtttt atcattagac
    52081 gggggtgtag ttgaagttgg tgctgcttta tcatgattgg gtattacctt ttgtgccgga
    52141 atggatggtt gtgatttatc aacatcattc gattgtgacg ttgtcgattg tgatccatta
    52201 cgttcagcat gatgggtatt ttgaacctga tcattttgac gttcagtatt attagtcgca
    52261 ttattttggt gtgactgttt agaaccttgt cgttcagaat cttgctttac ttgattatca
    52321 ttttgcgttc tgattgtttt actattttgt gttgattgat gcttcgatga ttgaagcgta
    52381 tctttacctt ctgtcactgt tccactttta ccatcctctt tatgcatatc gtctaattgc
    52441 tttcctgact gcatttgatg tgacgttatc gcattccctt gttctcccgg ttgatttagt
    52501 gtttcttttt gtgccgctgc attagttgat gttttttccg aggcttgtgc ttcatgtgat
    52561 gacacaacaa acattgtagc cgctaacact gacaccacgc ctattgaata ctttctaata
    52621 ctatactttc tttcttcttg tcctcttaac atataatcac ctcaaaagta tttttttgac
    52681 cccctcttga gaatgaaaaa caactgcacc ttcaatat
//
LOCUS       DAXWFK010000002        45050 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.002, whole genome shotgun sequence.
ACCESSION   DAXWFK010000002 DAXWFK010000000
VERSION     DAXWFK010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 45050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45050
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.002"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(249..1070)
                     /locus_tag="ACGEXS_000057"
     CDS             complement(249..1070)
                     /locus_tag="ACGEXS_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFF8011111.1"
                     /translation="MKKLFGLILVLTFAVVLAACGNGNKSGSDDKKITVGASPAPHAE
                     ILEKAKPLLEKKGYELDIKTINDYTTPNKLLDKGEIDANYFQHTPYLNTEKKDKGYKI
                     VSAGDVHLEPMAVYSKKYKSLKELPKGATVYVSNNPAEQGRFLKFFVDAGLIKIKKGV
                     KIEDAKFSDITENKKDIKFNNKQSAEFLPKIYQNEDADAVIINSNFAIEQKLNPKKDS
                     IAVESAKDNPYANLIAVKEGHQDDKKIKALIEVLQSKDIQDFINEKYNGAVIPAK"
     gene            complement(1088..1783)
                     /locus_tag="ACGEXS_000058"
     CDS             complement(1088..1783)
                     /locus_tag="ACGEXS_000058"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFF8011112.1"
                     /translation="MGKSFSEIINEMITMPNIQWPEVWTAIVETLYMTVVSTIFAFIL
                     GLILGVLLFLSAKGKSIGARLFYSIVSFIVNLFRAIPFIILILLLIPFTSLILGTISG
                     PTGALPALIIGAAPFYARLVEIAFKEIDKGVIEAAWSMGANTWTVIRKVLLPEAMPAL
                     VSGITVTAIALVGSTAVAGVIGAGGLGNLAYLTGFTRNQNDVILVSTVFILIIVFIIQ
                     FIGDWLTNKLDKR"
     gene            complement(1776..2801)
                     /locus_tag="ACGEXS_000059"
     CDS             complement(1776..2801)
                     /locus_tag="ACGEXS_000059"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFF8011113.1"
                     /translation="MIELKEVVKEYRTKNKEVLAVDHVNLSIRAGSIYGVIGFSGAGK
                     STLIRMFNHLEAPTSGEVIIDGDHIGQLSKNGLRAKRQKVSMIFQHFNLLWSRTVLKN
                     IMFPLEIAGVPRRRAKQKALELVELVGLKGREKAYPSELSGGQKQRVGIARALANDPT
                     VLLCDEATSALDPQTTDEILDLLLKIREQQNLTIVLITHEMHVIRRICDEVAVMESGK
                     VIEQGPVTQVFENPQHTVTKRFVKDDLNDDFETSLTELEPLEKDAYIVRLVFAGSTTT
                     EPIVSSLSTTYDIKINILEANIKNTKNGTVGFLVLHIPYISSVDFGKFEKELIERQVK
                     MEVLRHG"
     regulatory      complement(2890..2993)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(3051..3347)
                     /locus_tag="ACGEXS_000060"
     CDS             complement(3051..3347)
                     /locus_tag="ACGEXS_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFF8011114.1"
                     /translation="MNNSLDIKDVTTFYEEDKHLIFGYTPTCGTCKVSERMLDIANEI
                     LQLPLLKIDLNFYPQFCKDMQIMSTPILLLMNKDKEVKRIYAFKSVTDLLENLK"
     gene            complement(3340..3726)
                     /locus_tag="ACGEXS_000061"
     CDS             complement(3340..3726)
                     /locus_tag="ACGEXS_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFF8011115.1"
                     /translation="MAIVNKVIIVEGKSDKKRVQQVIAEPVNIICTHGTMSIDKLDDM
                     IESLYDKQVFVLADSDDEGDRIRNWFKRYLSESEHIFIDKTYCQVANCPKQYLAHVLS
                     KHGFTCKKETPLLPNINNERLVLVNE"
     gene            complement(4468..4758)
                     /locus_tag="ACGEXS_000062"
                     /pseudo
     CDS             complement(4468..4758)
                     /locus_tag="ACGEXS_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499392.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1963 domain-containing protein"
     gene            complement(4992..5654)
                     /locus_tag="ACGEXS_000063"
     CDS             complement(4992..5654)
                     /locus_tag="ACGEXS_000063"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8011116.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            6046..6105
                     /locus_tag="ACGEXS_000064"
     CDS             6046..6105
                     /locus_tag="ACGEXS_000064"
                     /inference="COORDINATES: protein motif:HMM:NF019858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HFF8011117.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            6163..6894
                     /gene="erm(A)"
                     /locus_tag="ACGEXS_000065"
     CDS             6163..6894
                     /gene="erm(A)"
                     /locus_tag="ACGEXS_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HFF8011118.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(7020..7802)
                     /locus_tag="ACGEXS_000066"
     CDS             complement(7020..7802)
                     /locus_tag="ACGEXS_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HFF8011119.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(7953..8330)
                     /locus_tag="ACGEXS_000067"
     CDS             complement(7953..8330)
                     /locus_tag="ACGEXS_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6262 family protein"
                     /protein_id="HFF8011120.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            complement(8337..10229)
                     /locus_tag="ACGEXS_000068"
     CDS             complement(8337..10229)
                     /locus_tag="ACGEXS_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFF8011121.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNVDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            complement(10226..11311)
                     /locus_tag="ACGEXS_000069"
     CDS             complement(10226..11311)
                     /locus_tag="ACGEXS_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8011122.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSKEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            complement(11445..11984)
                     /gene="radC"
                     /locus_tag="ACGEXS_000070"
                     /pseudo
     CDS             complement(11445..11984)
                     /gene="radC"
                     /locus_tag="ACGEXS_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692885.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            complement(11981..12688)
                     /locus_tag="ACGEXS_000071"
     CDS             complement(11981..12688)
                     /locus_tag="ACGEXS_000071"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFF8011123.1"
                     /translation="MVVLLSYSCSCIFSFLYQFISIEETSFDYLHRRSKCDYCNSSLK
                     WYELMPIISFLLLKGRCRNCRKRISLTHFLGETFALIPIVFIKYDFTYVNATLFITTY
                     VFLLIFTMTDITSLMLDCRLIIIYCIVSLSLSMIYPVAFIIISMTTHIFYFLFRAYIG
                     YGDVLLISALSLFFPLQFTIYVILFTFVIAGLVALITMIFKPIKLLPLVPFIFISFFI
                     NSLFYNDIHQFLGGVYF"
     gene            complement(12958..14229)
                     /locus_tag="ACGEXS_000072"
     CDS             complement(12958..14229)
                     /locus_tag="ACGEXS_000072"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFF8011124.1"
                     /translation="MNYLESLYWIHERTKFGIKPGVKRMEWMLAQFNNPQNNIKGIHV
                     GGTNGKGSTVAYLRTALVENGYEVGTFTSPFIETFNERISLNGVPISNDAIVELVSRI
                     KPVSEMMERETDLGVATEFEIITAMMFLYFGEIHPVDFVIVEAGLGIKNDSTNVFTPV
                     LSILTSIGLDHTDILGGTYLDIARDKGAIIKPNVPVIYAVKNEDALKYVRERAIEQHA
                     KPIELDREIVVVSQNDEFTYRYKDYELETIILSMLGEHQKQNAALAITALIELNEQGL
                     IELDFNKMIDGIESVRWTGRIEQVHDKPLIILDGAHNSESIDALIDTIKQYHDKEKVD
                     ILFSAINGKPINEMVKHLSLIAHTFYATEFDFPKALRKEEIVGSIENDEIQLVDDYVE
                     FIKNYQGDTLVITGSLYFISEVKSKLDFEAL"
     gene            complement(14242..16872)
                     /locus_tag="ACGEXS_000073"
     CDS             complement(14242..16872)
                     /locus_tag="ACGEXS_000073"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFF8011125.1"
                     /translation="MEMKPKYDPREVEAGRYEEWVKNGYFKPSEDKSKETYTIVIPPP
                     NVTGKLHLGHAWDTTLQDIITRMKRMQGYDTLYLPGMDHAGIATQAKVEAKLNEQGIT
                     RYDLGREKFLEQAWDWKEEYASFIRAQWAKLGLGLDYSRERFTLDEGLSKAVKKVFVD
                     LYNKGIIYRGERIINWDPKARTALSDIEVIHEDVQGAFYHFKYPYADGEGFIEIATTR
                     PETMLGDTAIVVNPNDERYKDVIGKTVILPIVGRELPILADEYVDIDFGSGAMKVTPA
                     HDPNDFEIGQRHQLENIIVMDENGKMNDKAGKYEGMDRFDCRKQLVKDLKEQDLVIKI
                     EDHVHSVGHSERSGAVVEPYLSTQWFVRMEDLAKRSLDNQKTDDRIDFYPQRFEHTFN
                     QWMENIRDWTISRQLWWGHQIPAWYHKETGEIYVGEEAPTDIENWQQDEDVLDTWFSS
                     ALWPFSTLGWPDLESEDFKRYYPTNALVTGYDIIFFWVARMIFQGLEFTDRRPFNDVL
                     LHGLVRAEDGRKMSKSLGNGVDPMDVIDEYGADSLRYFLATGSSPGHDLRYSTEKVES
                     VWNFINKIWNGARFSLMNIGEDFKVEDIDLSGNLSLADKWILTRLNETIATVTDLSDK
                     YEFGEVGRALYNFIWDDFCDWYIEMSKIPMNSNDEEQKQVTRSVLSYTLDNIMRMLHP
                     FMPFVTEKIWQSLPHEGDTIVKASWPEVRESLIFEESKQTMQQLVEIIKSVRQSRVEV
                     NTPLSKEIPILIQAKDKEIETTLSQNKDYLIKFCNPSTLNISTDVEIPEKAMTSVVIA
                     GKVVLPLEGLIDMDKEISRLEKELAKLQSELDRVDKKLSNENFVSKAPEKVINEEKRK
                     KQDYQEKYDGVKARIEQLKA"
     misc_binding    complement(16916..17217)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            17275..17835
                     /locus_tag="ACGEXS_000074"
     CDS             17275..17835
                     /locus_tag="ACGEXS_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFF8011126.1"
                     /translation="MNECAFGTKDPVYLDYHDHVWGQPLYDSKALFKLLALESQHAGL
                     SWLTILKKKEAYEEAFYDFEPEKVAQMTAQDIDRLMTFPNIVHHRKKLEAIVNQAQGY
                     LKIEQAYGSFSKFLWSYVNGKPKDLQYEHASDRITVDDTATQLSKDLKQYGFKFLGPV
                     TVFSFLEAAGLYDAHLQDCPSKPKHN"
     gene            complement(18058..19131)
                     /locus_tag="ACGEXS_000075"
     CDS             complement(18058..19131)
                     /locus_tag="ACGEXS_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFF8011127.1"
                     /translation="MAMIYRNNFIVFVLSFFISILLYSSHVLLPFMFGPIIASIICVK
                     VFKLDIKWPFLLSELGIVLLGVQIGSTFTKNVVMDIKNNWLSIIVVSISILLIAIVMA
                     FVFKKIARINTETAILSVIPGALTQMLVMAEQDKRANLLVVSLTQTSRIIFVVVLVPF
                     ISYFFHDGNMHANGKLTKVLPLSQVLNIGQIVILAIAIFIVYLIMSKIKFPTFQLLAP
                     LIVLIVWNFSTGLTFTLDHWLLNMAQLIYMIRIGVQIAHLLSDLKGRLAIAITIQNIM
                     LIIGALIMVYIIHFFDNNPINELFLGAAPGGVNQIVLVALATGADVAMITSYHIFRIF
                     FILFIIAPGINYFLKYRSNKRED"
     gene            complement(19165..19266)
                     /locus_tag="ACGEXS_000076"
     CDS             complement(19165..19266)
                     /locus_tag="ACGEXS_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011128.1"
                     /translation="MGGDLIVKVHICFVVKTASGYCYLNKREAQAAI"
     gene            complement(19327..20613)
                     /gene="hemL"
                     /locus_tag="ACGEXS_000077"
     CDS             complement(19327..20613)
                     /gene="hemL"
                     /locus_tag="ACGEXS_000077"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFF8011129.1"
                     /translation="MRYTKSEEAMKVAETLMPGGVNSPVRAFKSVDTPAIFMDHGKGS
                     KIYDIDGNEYIDYVLSWGPLILGHRDPQVISHLHEAIDKGTSFGASTLLENKLAQLVI
                     DRVPSIEKVRMVSSGTEATLDTLRLARGYTGRNKIVKFEGCYHGHSDSLLIKAGSGVA
                     TLGLPDSPGVPEGIAKNTITVPYNDLDALKIAFEKFGNDIAGVIVEPVAGNMGVVPPI
                     EGFLQGLRDITTEYGALLIFDEVMTGFRVGYHCAQGYFGVTPDLTCLGKVIGGGLPVG
                     AFGGKKEIMDHIAPLGNIYQAGTLSGNPLAMTSGYETLSQLTPETYEYFNMLGDILED
                     GLKRVFAKHNVPITVNRAGSMIGYFLNEGPVTNFEQANKSDLKLFAEMYREMAKEGVF
                     LPPSQFEGTFLSTAHTKEDIEKTIQAFDTALSRIVK"
     gene            complement(20661..21635)
                     /gene="hemB"
                     /locus_tag="ACGEXS_000078"
     CDS             complement(20661..21635)
                     /gene="hemB"
                     /locus_tag="ACGEXS_000078"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001633925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFF8011130.1"
                     /translation="MKFDRHRRLRSSATMRDMVRENHVRKEDLIYPIFVVEKDDVKKE
                     IKSLPGVYQISLNLLESELKEAYDLGIRAIMFFGVPNSKDDIGTGAYIHDGVIQQATR
                     IAKKMYDDLLIVADTCLCEYTDHGHCGVIDDHTHDVDNDKSLPLLVKTAISQVEAGAD
                     IIAPSNMMDGFVAEIRRGLDEAGYYNIPIMSYGVKYASSFFGPFRDAADSAPSFGDRK
                     TYQMDPANRLEALRELESDLKEGCDMMIVKPALSYLDIVRDVKNHTNVPVVAYNVSGE
                     YSMTKAAAQNGWIDEERVVMEQMVSMKRAGADMIITYFAKDICRYLDK"
     gene            complement(21638..22306)
                     /locus_tag="ACGEXS_000079"
     CDS             complement(21638..22306)
                     /locus_tag="ACGEXS_000079"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFF8011131.1"
                     /translation="MKPVVVMTQTNDMQSDLVSIIHKPFIDIKPLNFDIHLLNQRYDW
                     LIFSSKNAVKFFYKYLKGINVDNIAVIGSKTAQYCESLGIRVDFMPNDFSQEGFLKSF
                     NQTNQKILLPSSELARPLLLAALSKDNEVVKIDLYTSVPNKQNIQDVKEMIEHQQIDA
                     LTFSSSSAVRYYFNEGFVPKFKSYFAIGEQTARTIKSYQQPVTIAEIQTLESLIEKIL
                     ESRG"
     gene            complement(22328..23254)
                     /gene="hemC"
                     /locus_tag="ACGEXS_000080"
     CDS             complement(22328..23254)
                     /gene="hemC"
                     /locus_tag="ACGEXS_000080"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFF8011132.1"
                     /translation="MRKLVVGSRRSKLALTQSQQFIDKLKAVEPNLEIEIKEIVTKGD
                     RIVDKQLSKVGGKGLFVKEIQHELFEKNIDMAIHSLKDVPSVIPEGLTLGCIPDRELP
                     FDAYISKTHTPLSQLPEGSIIGTSSLRRGAQILSKYPNLEIKWIRGNIDTRLEKLQTE
                     DYDAIILAAAGLRRMGWSDDIVTSYLDRDTLLPAIGQGALGIECRSDDEELLTLLSKV
                     HNDEVAKCVTAERTFLAEMDGSCQVPIAGYATISDQNEIEFTGLIMTPDGKERFEYTM
                     NGTDPVELGKTVSNKLKEQGAYEIIKRLNEQH"
     gene            complement(23296..24111)
                     /gene="ccsA"
                     /locus_tag="ACGEXS_000081"
     CDS             complement(23296..24111)
                     /gene="ccsA"
                     /locus_tag="ACGEXS_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HFF8011133.1"
                     /translation="MQENLFIRFNEIILLIYLISIICYFYDFVQKSHKIRSLGIYLLG
                     IVWVLQTISLSIFIIQTRHIPLGSISDVFYTLSWLIISISLILNLIKVLNFSVFFLNL
                     IGLTLLGMNTFQPTHYTNKVQKIAVVDELLLVHIGLAVLSYVFFALAFVNALLYIMQY
                     RNLKEKRFDQKYFRIGSVATLESIVFYSTLSGWIILIFSIILGTQWGVISVGERIFID
                     PKVILSSIITVLYGSYILLRINKWLNSRYLIYYNIILFCLNMINLFFATHFVN"
     gene            complement(24133..25479)
                     /gene="hemA"
                     /locus_tag="ACGEXS_000082"
     CDS             complement(24133..25479)
                     /gene="hemA"
                     /locus_tag="ACGEXS_000082"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFF8011134.1"
                     /translation="MHFIAISINHRTADVALREQVAFRDDALRIAHEDLYETKSILEN
                     VILSTCNRTEVYAVVDQIHTGRYYIQRFLARAFGFEVDDIKAMSEVKVGDEAVEHLLR
                     VTSGLDSIVLGETQILGQIRDAFFLAQSTGTTGTIFNHLFKQAITFAKRAHNETDIAD
                     NAVSVSYAAVELAKKVFGKLKSKQAIIIGAGEMSELSLLNLLGSGITDITVVNRTIEN
                     AMKLAAKHQVKYDELSSLPNLLESADIVISSTSAQSYIITNEMIERIAENRKQDSLVL
                     IDIAVPRDIEPGISAITNIFNYDVDDLKGLVDANLRERQLAAATISEQIPAEIHAHNE
                     WISMLGVVPVIRALREKAMAIQAETMDSIDRKLPGLSERERKIISKHTKSIINQMLKD
                     PIKQAKELSSDKKSNEKLELFQNIFDIEAECPHEQAKQQKESKVKEISARRIFSFE"
     gene            complement(25696..26286)
                     /gene="yihA"
                     /locus_tag="ACGEXS_000083"
     CDS             complement(25696..26286)
                     /gene="yihA"
                     /locus_tag="ACGEXS_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFF8011135.1"
                     /translation="MKVNPNNIELIISAVKEEQYPETELSEVALSGRSNVGKSTFINS
                     MIGRKNMARTSQQPGKTQTLNFYNIDEQLIFVDVPGYGYAKVSKTQREKFGKMIEEYI
                     TKRENLQLVIQLVDLRHDPTQDDILMYNYLKHFDIPTLVICTKEDKIPKGKVQKHIKN
                     IKTQLDMDPDDTIVSYSSIQNNKQQQIWNLIEPYIS"
     gene            complement(26440..27702)
                     /gene="clpX"
                     /locus_tag="ACGEXS_000084"
     CDS             complement(26440..27702)
                     /gene="clpX"
                     /locus_tag="ACGEXS_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFF8011136.1"
                     /translation="MFKFNEDEENLKCSFCGKDQDQVKKLVAGSGVYICNECIELCSE
                     IVEEELAQNTSEAMTELPTPKEIMDHLNEYVIGQEKAKKSLAVAVYNHYKRIQQLGPK
                     EDDVELQKSNIALIGPTGSGKTLLAQTLAKTLNVPFAIADATSLTEAGYVGDDVENIL
                     LRLIQAADFDIDKAEKGIIYVDEIDKIARKSENTSITRDVSGEGVQQALLKILEGTTA
                     SVPPQGGRKHPNQEMIQIDTTNILFILGGAFDGIEEVIKRRLGEKVIGFSSNEADKYD
                     EQALLAQIRPEDLQAYGLIPEFIGRVPIVANLETLDVTALKNILTQPKNALVKQYTKM
                     LELDDVDLEFTEEALSAISEKAIERKTGARGLRSIIEESLIDIMFDVPSNENVTKVVI
                     TAQTINEETEPELYDAEGNLINNSKTSA"
     gene            complement(27853..29154)
                     /gene="tig"
                     /locus_tag="ACGEXS_000085"
     CDS             complement(27853..29154)
                     /gene="tig"
                     /locus_tag="ACGEXS_000085"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFF8011137.1"
                     /translation="MTATWEKKEGNEGLLTVTVPAEKVNKALDQAFKKVVKQINVPGF
                     RKGKVPRPIFEQRFGVEALYQDAIDILLPDAYGEAIDETDIKPVAQPEVSVTQIEKGK
                     DFIFEATVTVEPEVKLGDYKGLEIEKQETELSDDELQEAIDHSLGHLAEMVVKEDGVV
                     ENGDTVNIDFSGSVDGEEFEGGQAEGYDLEIGSGSFIPGFEEQLEGMKVDEEKDVVVT
                     FPEEYHAEELAGKEATFKTKVNEIKFKEVPELTDEIANELDAEANTVDEYKENLRKRL
                     AEQKATDAENVEKEEAITKATDNTTIDIPEAMVNTELDRMVSEFAQRIQQQGLDLQTY
                     FQISGQDETQLREQMKDDAEQRVKTNLTLTAIAEAEKIEATDEDIDKELEKMSKQFNI
                     SVEDIKNTLGNTDIIKNDVRIQKVIDLLRDNAKFVEGTKED"
     gene            complement(29202..29312)
                     /locus_tag="ACGEXS_000086"
     CDS             complement(29202..29312)
                     /locus_tag="ACGEXS_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011138.1"
                     /translation="MQNNTQHFNIVLTKKVDLQIYKRCDFNVCIGNRPMI"
     gene            complement(29317..30246)
                     /locus_tag="ACGEXS_000087"
     CDS             complement(29317..30246)
                     /locus_tag="ACGEXS_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011139.1"
                     /translation="MSDIIQFPNSSKKLYKDIKRAEQDQNYDLMYEYIVQYERQFELT
                     EEIAMMKCRMLYETGSFLELREETIVLLKTGIQQYDALMIYYVKSLIGLGQYFEAVEV
                     IHQIIDEVKDHKTRMALHPLKEFAKSKLIEDEKRLTQSLADFDTLSMREQTHLILKLI
                     DNGHFQFQETVLYILKSNTYSYNLISLMIEYLRFANCTQELTIEKYGMDVTFVPANLK
                     GLEHTTLKEKVIPNVMETLNDGALHIAEEAHHIMNNHSIMIYPIDIETLFETNKWINA
                     YECYFKNMLGLKCELQSIDAFNFIQQLDLNNNS"
     gene            complement(30265..30873)
                     /locus_tag="ACGEXS_000088"
     CDS             complement(30265..30873)
                     /locus_tag="ACGEXS_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFF8011140.1"
                     /translation="MSKFDEQIIVVPREIIFNNEKNTFNGFLNKNKPEGQNIFDALSQ
                     YEVKRRGDMEEDPSYKQLISYCLLENEHGEILVYERLSGGGEARLHGQSSIGVGGHMN
                     DVPGTESINEVLRVNAQRELEEEVGLSEQDSQNMEYIGFINDDNNEVGKVHIGVVFKI
                     TVSTNDVEAKETDTLRIKWVEKGNIESYDDFETWSALILQDL"
     gene            complement(31015..31371)
                     /gene="rplT"
                     /locus_tag="ACGEXS_000089"
     CDS             complement(31015..31371)
                     /gene="rplT"
                     /locus_tag="ACGEXS_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFF8011141.1"
                     /translation="MPRVKGGTVTRARRKKTIKLAKGYFGSKHTLYKVAKQQVMKSGQ
                     YAFRDRRQRKRDFRKLWITRINAAARQHEMSYSRLMNGLKKAGIDINRKMLSEIAISD
                     EKAFAQLVTKAKDALK"
     gene            complement(31418..31618)
                     /gene="rpmI"
                     /locus_tag="ACGEXS_000090"
     CDS             complement(31418..31618)
                     /gene="rpmI"
                     /locus_tag="ACGEXS_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFF8011142.1"
                     /translation="MPKMKTHRGAAKRVKRTASGQLKRSRAFTSHLFANKSTKQKHQL
                     RKARLVSKSDMKRVKQLLAYKK"
     gene            complement(31647..32174)
                     /gene="infC"
                     /locus_tag="ACGEXS_000091"
     CDS             complement(31647..32174)
                     /gene="infC"
                     /locus_tag="ACGEXS_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFF8011143.1"
                     /translation="MSTIAKDQTQINDKIRAKELRLIGQDGEQIGVKSKREALEMAER
                     VDLDLVVVAPNAKPPVARIMDYGKFKFEQQKKEKEMKKKQKIINVKEIRLSPTIEEHD
                     FQTKLKNGRKFLTKGDKCKVSIRFRGRAITHKEIGQRVLEKYADECKDIATVEQKPKM
                     DGRQMFIMLAPTAEK"
     misc_feature    complement(32203..32328)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            complement(32403..33896)
                     /locus_tag="ACGEXS_000092"
     CDS             complement(32403..33896)
                     /locus_tag="ACGEXS_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFF8011144.1"
                     /translation="MSKVQNESNNVVKRGLKDRHISMIAIGGCIGTGLFVTSGGAIHD
                     AGALGALIGYAIIGIMVFFLMTSLGEMATYLPVSGSFSTYATRFVDPSLGFALGWNYW
                     FNWVVTVAADITIAAQVIQYWTPLQGIPAWAWSALFLVIIFSLNSLSVRVYGESEYWL
                     ALIKVVTVIVFIAIGLLTIVGIMGGHVVGFEIFNKGEGPILGGNLGGSLLSILGVFLI
                     AGFSFQGTELIGITAGESENPERAVPKAIKQVFWRILLFYILAIFVIGMLIPYDSSAL
                     MGGSDNVATSPFTLVFKNAGFAFAASFMNAVILTSVLSAGNSGMYASTRMLYSMSKDK
                     LAFEAFGKTNKNGVPYMSLLVTAIIVVIIFVLQSLTKGAYEYIVAASGLTGFIAWVGI
                     AVSHYRFRRAFDKQNYDKSKLKYTAKLFPFGPLFAGFLCIVVIIGQDVDFIKTGDFNF
                     SRFLITYMGIPVFLAFFIYHKLRYKTKKIPLEQVDLRQDVSMDEIKG"
     regulatory      complement(34041..34216)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            complement(34322..36259)
                     /gene="thrS"
                     /locus_tag="ACGEXS_000093"
     CDS             complement(34322..36259)
                     /gene="thrS"
                     /locus_tag="ACGEXS_000093"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFF8011145.1"
                     /translation="MEQINIQFPDGNKKAFDKGTTTEDIAQSISPGLRKKAVAGKFNG
                     QLVDLTKPLETDGSIEIVTPGSEEALEVLRHSTAHLMAHAIKRLYGNVKFGVGPVIEG
                     GFYYDFDIDQNISSDDFEQIEKTMKQIVNENMKIERKVVSRDEAKELFSNDEYKLELI
                     DAIPEDENVTLYSQGDFTDLCRGVHVPSTAKIKEFKLLSTAGAYWRGDSNNKMLQRIY
                     GTAFFDKKELKAHLQMLEERKERDHRKIGKELELFTNSQLVGAGLPLWLPNGATIRRE
                     IERYIVDKEVSMGYDHVYTPVLANVDLYKTSGHWDHYQEDMFPPMQLDETESMVLRPM
                     NCPHHMMIYANKPHSYRELPIRIAELGTMHRYEASGAVSGLQRVRGMTLNDSHIFVRP
                     DQIKEEFKRVVNMIIDVYKDFGFEDYSFRLSYRDPEDKEKYFDDDDMWNKAENMLKEA
                     ADELGLSYEEAIGEAAFYGPKLDVQVKTAMGKEETLSTAQLDFLLPERFDLTYIGQDG
                     EHHRPVVIHRGVVSTMERFVAFLTEETKGAFPTWLAPKQVQIIPVNVDLHYDYARQLQ
                     DELKSQGVRVSIDDRNEKMGYKIREAQMQKIPYQIVVGDKEVENNQVNVRQYGSQDQE
                     TVEKDEFIWNLVDEIRLKKHR"
     gene            complement(36457..36579)
                     /locus_tag="ACGEXS_000094"
     CDS             complement(36457..36579)
                     /locus_tag="ACGEXS_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011146.1"
                     /translation="MFDENMVNKLDDYYFIYGICNEYPDQDRYLKQRYLIHKLY"
     gene            complement(36672..37592)
                     /gene="dnaI"
                     /locus_tag="ACGEXS_000095"
     CDS             complement(36672..37592)
                     /gene="dnaI"
                     /locus_tag="ACGEXS_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFF8011147.1"
                     /translation="MKQFKSIINTSQDFEKRIEKIKKEVINDPDVKQFLEAHRAELTN
                     AMIDEDLNVLQEYKDQQKHYDGHKFADCPNFVKGHVPELYVDNNRIKIRYLQCPCKIK
                     YDEERFEAELITSHHMQRDTLNAKLKDIYMNHRDRLDVAMAADDICTAITNGEQVKGL
                     YLYGPFGTGKSFILGAIANQLKSKKVRSTIIYLPEFIRTLKGGFKDGSFEKKLHRVRE
                     ANILMLDDIGAEEVTPWVRDEVIGPLLHYRMVHELPTFFSSNFDYSELEHHLAMTRDG
                     EEKTKAARIIERVKSLSTPYFLSGENFRNN"
     gene            complement(37592..38992)
                     /locus_tag="ACGEXS_000096"
     CDS             complement(37592..38992)
                     /locus_tag="ACGEXS_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HFF8011148.1"
                     /translation="MGRQAFEFGLRPKDQFKVMQHFDLNTNHLEVLNRLYTPLIGTQA
                     VGLYHFMTQFVKESHNETLILSHYIFMNELKINLLEFRQQMDLLEAIGLLKAFVKHDE
                     QETQFVYQLIQPPSAHLFFNDPMLSIFLYSEVEHRRFHELKKYFEYQQIDLSEFKQVT
                     RQFTDVFKVPSTKIDIDTSDIPINEPYQGIDLSNESFDFEMLRQMLGKHFISQDIVTK
                     DAKRLITQLATLYGLTADGMKHVILNSITSGQQLSFEEMRKQARSYYLMEHENQMPKL
                     QVKSPATSSSAGKSSEVNPKPQSDEWFELLEQTSPIDMLASWSESEPTISQKTMVEEL
                     IEREKMSFGVINILLQFVMLKEDMKLPKAYILEIASNWKKKGIKTAKEAYNYAKKVNQ
                     PKNEGSSGNYQKRGSYYGQRNRISKEKTPKWLENRDKPSEEDSAKDNSVDDQQLEQDR
                     QAFLDKLSKKWEEDSQ"
     gene            complement(38993..39463)
                     /gene="nrdR"
                     /locus_tag="ACGEXS_000097"
     CDS             complement(38993..39463)
                     /gene="nrdR"
                     /locus_tag="ACGEXS_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFF8011149.1"
                     /translation="MKCPKCNSTQSKVVDSRHADELNAIRRRRECENCGTRFTTFEHI
                     EVSQLIVVKKDGTREQFSREKILNGLVRSCEKRPVRYQQLEDITNKVEWQLRDEGHTE
                     VSSRDIGEHVMNLLMHVDQVSYVRFASVYKEFKDVDQLLASMQGILSENKRSDA"
     gene            complement(39673..40698)
                     /gene="gap"
                     /locus_tag="ACGEXS_000098"
     CDS             complement(39673..40698)
                     /gene="gap"
                     /locus_tag="ACGEXS_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFF8011150.1"
                     /translation="MSTNIAINGMGRIGRMVLRIALQNKNLNVVAINASYPPETIAHL
                     INYDTTHGKYNLKVEPIENGLQVGDHKIKLVADRNPENLPWKELDIDIAIDATGKFNH
                     GDKAIAHIKAGAKKVLLTGPSKGGHVQMVVKGVNDNQLDIEAFDIFSNASCTTNCIGP
                     VAKVLNNQFGIVNGLMTTVHAITNDQKNIDNPHKDLRRARSCNESIIPTSTGAAKALK
                     EVLPELEGKLHGMALRVPTKNVSLVDLVVDLEKEVTAEEVNQAFENAGLEGIIEVEHQ
                     PLVSVDFNTNPNSAIIDAKSTMVMSGNKVKVIAWYDNEWGYSNRVVDVAEQIGALLTS
                     KETVSAS"
     gene            complement(40868..41491)
                     /gene="coaE"
                     /locus_tag="ACGEXS_000099"
     CDS             complement(40868..41491)
                     /gene="coaE"
                     /locus_tag="ACGEXS_000099"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500299.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFF8011151.1"
                     /translation="MPKVIGLTGGIASGKSTVSELLSVFGFKVVDADKAAREAVKKGS
                     KGLAQVREVFGDEAIDENGEMNRRYMGDLVFNHPEKRLELNAIIHPIVRDIMEEEKQE
                     YLKQGYNVIMDIPLLFENELENTVDEVWVVYTSESIQMDRLMQRNNLSLEDAKARVYS
                     QISIDKKSRMADHVIDNLGDKLELKQNLERLLEEEGYIEKLNYGEED"
     gene            complement(41507..42379)
                     /gene="mutM"
                     /locus_tag="ACGEXS_000100"
     CDS             complement(41507..42379)
                     /gene="mutM"
                     /locus_tag="ACGEXS_000100"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFF8011152.1"
                     /translation="MPELPEVEHVKRGIEPYVINQKIEHVIFSDKVIEGKAQGKETII
                     KGIELDTFKTLSEGYTITNVERRSKYIVFQLDNKREQRTLISHLGMAGGFFIVDELED
                     IMIPNYRKHWHVIFELSNDKKLIYSDIRRFGEIRNVASVASYPSFLEIAPEPFSNEAL
                     TYYLNRIHQQSNKNKPIKQVILDHKVIAGCGNIYACEALFRAGVLPDKKVKDLTHQQQ
                     EMVFYYVREVLEEGIKYGGTSISDYRHADGKTGEMQLHLNVYKQPVCKVCGSQIETKI
                     IATRNSHYCPVCQK"
     gene            complement(42395..45025)
                     /gene="polA"
                     /locus_tag="ACGEXS_000101"
     CDS             complement(42395..45025)
                     /gene="polA"
                     /locus_tag="ACGEXS_000101"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFF8011153.1"
                     /translation="MNKLVLIDGNSLSFRAFYALPLLSNKAGIHTNAVYGFAMLLEKI
                     LKEEKPNHFLVAFDAGKTTFRHEKYSEYKGGRQKTPPELSEQFPYIRQLLDAYHIKRY
                     ELDNYEADDIIGTLSKEADKAGFQTIIITGDRDLTQLATDNVTIYYTKKGVTDVDHYT
                     PDFIAEKYNGLTPNQIIDMKGLMGDTSDNIPGVAGVGEKTAIKLLNQFDTVEGVYEHL
                     DEISGKKLKEKLQNSKEDALMSKELATINVDSPIEVKLEDTLMTHQDEQQEKIELFKK
                     LEFKQLLADIDQSASVEDAIEKTFEIETSFDNIDFTSLKEAAIHFELDGGNYLRNNIL
                     KFSLFTGEKHIVINADDINNYVELVSWLENPNSKKVVYDAKKTYVASHRLGIDIQNIS
                     FDIMLASYIIDPSRTISDVQSVVSLYGQSFVKDDVSIYGKGKKFKVPEDDVLNPYVAS
                     ITDAIYFAKPNMDKQLEEYNQVELLADLELPLAKILSEMEEIGIFTDVHDLEEMEKEI
                     QEKLDVLIRNIHDAAGEDFNINSPKQLGVVLFETLQLPVIKKTKTGYSTAVDVLEQLQ
                     GEHPIIDYILEYRQLSKLQSTYVEGLQKVISDDQRIHTRFNQTLAQTGRLSSVDPNLQ
                     NIPVRLEEGRKIRKAFKPTSKDSVILSADYSQIELRVLAHITQDESMKEAFINGDDIH
                     TATAMKVFGVEADQVDSLMRRQAKAVNFGIVYGISDYGLSQSLGITRKKAKAFIDDYL
                     ASFPGVKQYMSDIVKDAKALGYVETLLHRRRYIPDITSRNFNLRGFAERTAMNTPIQG
                     SAADIIKLAMVKFAQKMKETTYQAKLLLQVHDELIFEVPKSEVDSFSEFVEEIMENAL
                     QLDVPLKVDSSYGATWYDAK"
BASE COUNT        14158 a         8591 c         6139 g        16162 t
ORIGIN      
        1 ttttataact agcgaaatag attcatcatt aacttgaggg agtgggactg aaataataaa
       61 gaatcactaa tgatttatga tgtattagtc actagccatg tgaaattaaa aataagaata
      121 aatgagtagc acgcatgcat ataggatttt actttatccg taatagcatc tcattcctaa
      181 atatcatata aatacctgtt taaattaaaa agcccagcaa catcacgtta ctgagccatt
      241 aatatgattt atttagcagg aataactgca ccattgtatt tttcattaat gaagtcttga
      301 atatctttag attgtaatac ttcaattaat gctttgattt tcttatcatc ttgatgtcct
      361 tctttaacag caattaagtt tgcataagga ttatctttcg cactttctac agcaatagaa
      421 tcttttttag gatttagttt ttgttcgatt gcaaagttcg aattaatgat aacagcatca
      481 gcgtcttcat tttgataaat tttaggtaag aattctgctg attgtttatt attaaactta
      541 atatcttttt tattctctgt aatatcacta aacttagcat cttcaatttt tacgcctttt
      601 ttgattttaa ttaaacctgc atcaacgaag aattttaaga aacgtccttg ttcagctgga
      661 ttattagaca catagactgt tgcacctttt ggtaattctt ttaaactttt atacttttta
      721 gagtatacag ccataggttc taagtgaaca tcaccggcac ttacgatttt gtaaccttta
      781 tcctttttct ctgtgtttaa atatggtgta tgttggaaat agtttgcgtc aatttcacct
      841 ttgtctagta atttattagg tgtagtgtaa tcgttaattg ttttaatatc tagttcataa
      901 ccttttttct ctaataatgg ttttgctttt tctaaaattt cagcatgtgg tgctggtgaa
      961 gcacctactg ttattttctt gtcgtcactg ccacttttgt ttccattacc gcaagctgct
     1021 aatacaactg caaatgttaa tactaaaata agaccaaata attttttcat aaaatgaaac
     1081 ccccaattta tcgtttatca agtttatttg taagccaatc cccaatgaat tggattataa
     1141 atacaataat taaaataaaa actgttgata ctaaaatgac atcattttga tttcgagtga
     1201 aacctgttaa gtatgctaaa tttcctaaac caccggcacc aattacacct gcaactgctg
     1261 ttgaaccaac taaagcgatt gctgtaactg taatgccaga cactagcgct ggcatagctt
     1321 caggtaaaag gactttacga attactgtcc aagtattagc gcccattgac caagccgctt
     1381 cgatgacacc tttatcaatt tctttaaaag caatttctac gagccttgca taaaacggtg
     1441 ctgcgccaat gatcaaggct ggtaacgcac ctgtcggacc acttatcgtt ccaagtatca
     1501 aacttgtaaa tggaattaat aataaaatta aaataataaa tggtatcgct ctaaataagt
     1561 taacaatgaa agaaacgata gaataaaata accttgcacc gatagactta cctttagcag
     1621 acaagaataa taacacacct aaaataagac caagtataaa tgcaaatata gttgagacga
     1681 ctgtcatgta tagtgtttcg actattgcag tccaaacttc tggccactga atattaggca
     1741 ttgtaatcat ttcatttata atttcactaa atgatttacc catgtcttaa cacctccatt
     1801 ttaacttgtc gctcaattaa ctctttttcg aattttccga aatctacact tgaaatatat
     1861 ggaatatgca gaactaaaaa gccgactgtt ccattttttg tatttttaat atttgcttct
     1921 aaaatattaa ttttaatatc ataggtagtt gatagactcg atacaatagg ctcggttgtt
     1981 gttgaaccag cgaaaactaa tctaacgata tatgcatctt tttctaatgg ctctaattct
     2041 gttaaagatg tttcgaaatc atcatttaaa tcgtctttca caaatcgttt tgtcacagtg
     2101 tgttgcggat tttcaaaaac ctgtgtcacc ggtccttgtt ctatcacttt accactttcc
     2161 ataactgcaa cttcatcaca aatacgacga atgacatgca tttcatgcgt aattagtaca
     2221 attgttaaat tttgttgttc tctaattttt agtagtagat ctaaaatttc atctgttgtt
     2281 tgcggatcaa gtgcacttgt tgcctcatca caaagcaaga ccgttggatc attagctaac
     2341 gctcgtgcaa tcccaacacg ttgcttttgt ccacctgata actctgatgg ataagccttt
     2401 tctctacctt ttaaaccgac gagttcgaca agttctaatg ctttttgctt agctctcctt
     2461 ctagggacac ctgcaatttc aagcggaaac ataatatttt ttaacacagt ccttgaccat
     2521 aacaaattaa aatgttggaa gatcatactt actttttgtc tttttgctct taatccattt
     2581 ttggacaatt gacctatatg gtctccatct ataataactt cacctgatgt aggcgcttct
     2641 aaatgattaa acattcgaat caaagtactt tttcctgctc cagaaaaacc aatgacgcca
     2701 taaatcgatc ctgctcgaat cgataaatta acgtgatcta cagcaaggac ttctttattt
     2761 ttagtccgat attctttaac aacttctttt aattcaatca cgttgattcc tccctgtgtt
     2821 gcttaataaa ataaaaaatg ctttctcaat atcgatagaa aaattgagaa agcaatagta
     2881 gtattgtttt tctcatcttc aaaagttaaa actttatgtg aattggcacc atttctatat
     2941 aagacggttg ccgggcttcg tagggcacat ccctccacca ctctcgataa gagtttacgc
     3001 atcatttaat ttgtattaat cctaacacct tagtaaaatt tcgtcaataa ctattttaaa
     3061 ttttctaaca aatcagtcac cgatttaaat gcataaattc gttttacttc tttatcttta
     3121 ttcatcaaca ataaaatcgg cgtagacatg atttgcatat ctttacaaaa ctgaggataa
     3181 aagtttaaat ctattttcaa taatggtaac tgcaatattt cattagcaat gtctaacatt
     3241 ctttctgaaa ccttacaagt accacacgtt ggtgtataac caaagattaa atgtttgtct
     3301 tcctcataaa atgtagttac atctttgatg tctaatgaat tattcattta ctaaaactaa
     3361 cctttcatta tttatattcg gtaaaagagg tgtttctttc ttacaagtaa agccatgttt
     3421 tgaaagtaca tgcgccaaat attgtttggg gcaattcgca acttgacagt aagttttatc
     3481 aataaatata tgttcacttt cactcaaata acgtttaaac caatttctaa ttcgatctcc
     3541 ttcgtcatca gaatcggcta atacaaaaac ttgtttatca tacagtgatt ctatcatatc
     3601 atcaagctta tctatactca ttgttccatg agtacaaata atattgactg gttctgcaat
     3661 aacctgttgc accctttttt tatcagattt tccttcaaca attatcactt tatttacaat
     3721 agccatcatc atcacccttt aaaatcaata aacatctgtc actgtatcat ttcacaaaat
     3781 tggtatgaat aaaacataaa tcacaaaaaa tttaaactag cttaatataa taattacaaa
     3841 ctcaatgttt gactagctgg aacatttaac ataagcagac aaaggctaag tcaaaaatca
     3901 acatcctaaa atctacaatg ttatattaac aatagttaac caaaagaaaa tacacctata
     3961 acaaactttt caattatagc ggggccccaa cacagaagct gatggtaagt cagcttacaa
     4021 taatgtgcaa gttggcgggg ccccaacata aagaaatact ttttctttag aaattagtat
     4081 ttcttatgca tgagttttac tcatgtattc ctatttttaa atacacatta gctgtggctt
     4141 atgaaaacag gctgggacat aaatcaatgt tctatgctct acgaagttat attggcagta
     4201 gttgactgaa cgaaaatgcg cttgtaacaa gcttttttca attctagtca ggggccccaa
     4261 cacagagaat ttcgaaaaga aattctacag gcaatgcaag ttggggatgg gccccaacaa
     4321 agagaaattg gattcccaat ttctacagac aatgcaagtt ggggtgggac gacgaaataa
     4381 attttgcgaa aatatcattt atgtcccact ccctagattg atctatagat actacactta
     4441 ttaaagtaat atatttttat gattctctta gctgcaatcc catgaataca tgtaatcatc
     4501 aaacttcata gcctcaaggt cagtagattt cactaagata ttggcaatac ctaaatcacc
     4561 ccatataatc gaagaattac catctcttgt agaatcaatt tgcagtaaga ctctatcata
     4621 cattttcagt tcttgatcgt tcgttcttgg atctatttgg tcaaaatatg gataaccacc
     4681 aagttgatta aatggttcct tagcagtaaa tgtgttacac acaggttcca tcaattcatc
     4741 ataaccacca aacattgtac atcttatcaa gagtaaatta caaaagaatc atttaaatca
     4801 atactttcac tttgaataca tgtatttgaa gtggaaggta cttatttcaa aaatagtaaa
     4861 acctgtatct taaattactt aatagtaaca taagatacag gctgattttt tattcattgt
     4921 tatttatact aaagcacccg atagctctga aaacaatcac aaattcaact tttcaaagcc
     4981 acagctttaa gttattttgt ccagacaacc cccattgccc aacccatttt atggaattgg
     5041 catccaggca acaacttttc atataaatcg taataatttt gttcagatag gtacttatct
     5101 gaagctaaat gctcaagcca tgatttagat gtgttgtgat tataaattct aatcgcattt
     5161 tttattccaa atttgaaaca atgcggtaaa aactcttgga ttgcccctaa tttataaaca
     5221 taggtaggcg gtgtctcgac ttcagataca ttatcaagaa taactattct tccttcctca
     5281 ttcaacagtt ccttcatttg ctgtattacg ctggctatat catccaaatg atgaaaggtt
     5341 gttcggctta caataaaatc aaacttctca ttaaaattaa gttgttctgc attcatattc
     5401 agatagaccg tatttgttag ttgacgttta gatttggcaa gatcgagcat ttgattagaa
     5461 atatcaatcc ctaccacttc atcataataa cttgctaatt tctccactaa caaacccgag
     5521 ccacatccga tatctaatgc tctgcctttc tttggagaca tattagacac aaagaatgaa
     5581 taatcattca aaagctcatt cacgaaatcg taatcttctg caaccttatc aaactgtgat
     5641 tctattgtat tcaaaaagat cccccattcc tactttatcg acattctttc attacttacc
     5701 actttagatg ttttttcgtt ggggataaaa cttcccttta gacaatttta tccaaagaca
     5761 atacaacagt gcaactttat taaagtcact gtcctttatc gcagccttta ctttttagta
     5821 aagacagtgg cttctcttat caagtttcaa aacatattat tttgaagaaa acgtccatct
     5881 gaagtgtcaa gtgcaaaatt acatataaag gtttattcta aaatgaaaag atgatacaat
     5941 catattcagt tacataagga ggtttcaatt atgtgcacca gtatcgcagt agtagaaatt
     6001 actttatctc attcataatg aaaaaaatgg aaaggagata aaagtatggg tactttttct
     6061 atatttgtta ttaataaagt tcgttatcaa ccaaatcaaa attaattggt tataatgaac
     6121 gcttaatgtc agttcattat aaccagtaag gagaaggtta taatgaacca gaaaaaccct
     6181 aaagacacgc aaaattttat tacttctaaa aagcatgtaa aagaaatatt gaatcacacg
     6241 aatatcagta aacaagacaa cgtaatagaa atcggatcag gaaaaggaca ttttaccaaa
     6301 gagctagtca aaatgagtcg atcagttact gctatagaaa ttgatggagg cttatgtcaa
     6361 gtgactaaag aagcggtaaa cccctctgag aatataaaag tgattcaaac ggatattcta
     6421 aaattttcct tcccaaaaca tataaactat aagatatatg gtaatattcc ttataacatc
     6481 agtacggata ttgtcaaaag aattaccttt gaaagtcagg ctaaatatag ctatcttatc
     6541 gttgagaagg gatttgcgaa aagattgcaa aatctgcaac gagctttggg tttactatta
     6601 atggtggaga tggatataaa aatgctcaaa aaagtaccac cactatattt tcatcctaag
     6661 ccaagtgtag actctgtatt gattgttctt gaacgacatc aaccattgat ttcaaagaag
     6721 gactacaaaa agtatcgatc ttttgtttat aagtgggtaa accgtgaata tcgtgttctt
     6781 ttcactaaaa accaattccg acaggctttg aagcatgcaa atgtcactaa tattaataaa
     6841 ctatcgaagg aacaatttct ttctattttc aatagttaca aattgtttca ctaaattaaa
     6901 gtaataaagc gttctctaat ttcacaagag gacgctttat tcttcccaaa aattgttcaa
     6961 tatttatcaa taaatcagta gttttaaaag taagcacctg ttattgcaat aaaattagcc
     7021 taattgagag aagtttctat agaatttttc atatacttaa cgagtgcttt cacctttgaa
     7081 tatagtcctt cccacttatc atcacactct ccccgatagc cttttctagc tatatccagt
     7141 aaagttacat gctctttagg taaaagaggt atagcccatt ctgcagcgac atctttcgag
     7201 gtaatttcac cagtagtcac tgtttgccac attcgagcta gggttaaaat tacattacgc
     7261 tcatcacctt ttatcccctc aattagttct ggcaaagaat ccttaattgc tcttcgaata
     7321 tctgtcaaag gtacggagac aagtatactt gaagaatcag gaccaaatag agaaatacta
     7381 ttctttcttg cttgtgctaa aacaatagcc aaatcaggat catagcttgg ttcctgaatt
     7441 tgtccattct caaattcacc cctgagccac tcaccgtata taaattctct ttttggagga
     7501 tattgccaag ggacaacttc actcctattt ataaccgtaa cttcaagtgg tctaacagaa
     7561 tccgtatttc caatctttcc tgatatagtc attagtcttt ctgttagttt ttttcgagtt
     7621 aattgaggta aactatgatt cacgacgact agaacatcta catcgctgtt aatgcgtaaa
     7681 ccaccattta ctgctgaacc aaatagatat actccaacta ttgaacttcc aaataaatct
     7741 tttacgattt ttaatgtttg aatcgcttga tttggtattt ttccgttaat caaattgctc
     7801 atgatttcac ctcgttgatt atgttcatat aaagtttata ttgatactca atttacttac
     7861 cctagattgg acatatactt aaattactgt tcaataaagc tgaccgttag cgtttaagta
     7921 catcctttca caatttgtct acagattaat aattattctt tattatacag atctccatat
     7981 aatttttgaa tttggttctg taatttttta ttttcttttt ctaattccat tactcttctt
     8041 tttaaggttt taataaggat ttcctccgaa cgagaacttt tcttgggttt tgagactaca
     8101 tttgctgtta tttgacgctc acgaagggat tcgattcttt gcctaatatc gtgttcctta
     8161 taaagccatg atttagaaac attagcttcc tttgctattg aattaaaatt aataacttta
     8221 ccttcaatcg aaaatttaga aatcgctttg tctacttttt cccttgtctt ttttgatttc
     8281 tgcttcgcca aacgtacaat ttctgttgta tttctaactt gtttatccat tgataattac
     8341 cccgtcaaac ttccaatgat ttgttctaaa cgctctttaa cacggctatt agtctctact
     8401 tgtctttgcc attgtttatc cttagctatg gctaataact cttctgtacg ctctaactgt
     8461 tcttcgtgct gtggtaagaa ttgcttactg gtacagaagt gagtgcaatc taagcatgca
     8521 ttcgcatgtg gacaaccacc tgctactact ggcaatctac aataaccatt tggaagcact
     8581 tgtgcattta tatttttctt gaaccattga agctctacat catcgacttc attatcttca
     8641 tctagatcaa gcacatctcc attattggta accagttttt cctgaaattt agtaaattca
     8701 ttttttagag tttcatcaaa gatatgagcg tatctgcttg tcatttctgg gctttcatgc
     8761 cccaaaaatt tctgcacaat atgctggggc atcccgttgt taatcattct tgttcctact
     8821 gtatggcgaa aggcatgggc atggaatcta taaatctcac ctgatttatc cactatattt
     8881 tgctcataag ctaatttatt taactcacct ctaaatgttt cttgttttaa tggcgatcca
     8941 tcttttcttg gaaagaggta ttcactatct ggaaattcct ctgaaacttt atcttcccga
     9001 actttaataa gtaaagctac ctctttagat attggaacta tatgctcctt tttcattttc
     9061 cattgataat actttaaaaa gaaatctcca tctttgtcct ctaatagaca gccttttttc
     9121 aaggtgcaca attcacttat cctcattcca cattcttgaa caatcatagt catcgtagct
     9181 atatattcag gtaatttatc aagatgactg ttcaattgct ctaggacgaa ttcatctata
     9241 aagcgtggtt ttgctcttgg tattttcgga tagtcctcag aataaattaa tattttggaa
     9301 ggaacatcat cccattctag cctaagaagg gtactaaata gtccttccaa tatagagatc
     9361 ctcccagtta ttgtactagg ttttattccc atcatgttta gttcacttaa atatgcttca
     9421 atttccactc tcgttaattg gtgtactctc tgaacttgtt taaatttcat gtccagaaaa
     9481 ttaaagaact ctttaagtct ttgggcaata tcacttacat aggaaaagct atccacgttc
     9541 aatctcaact tacaatatct ttttacaagt tgtttaaaat atgtattccg aaacccttta
     9601 aagttaattg tatattcata ttgtgttggg ttaaccttat catctggcaa aggtaagtta
     9661 cgtctatccc aaacgtcttt atcccactcc tctccatcaa aataaaagtt ctcataaaac
     9721 tccataaatt gttttagatt agtaacatag taggaattag cttttacagg tgttttttct
     9781 tgattagcag taatcttata attagtagtg gtaattctaa caccccgttt tgtcaaataa
     9841 gttctatact ccgtcattgc tttttcaata ggaacttcag taattgaagt aatgctagga
     9901 tactttaaat ctaagaaatc taacatttta ttaattactg ttccttttct aatccagaca
     9961 gtttttgcat tccatattcc attgtttaaa tggtaaaaat aaaaatattt caattctgtt
    10021 cttaaccaca gatttttaac acgttcaaaa cgaacccaac gattccttaa agcaggattc
    10081 ttacttaatt ctatggcaga aggatgtgga cattttctta tatcccaact attattagcc
    10141 caaaacccct gcatttcttc attcattaca gctatttttt tgctaatctc actctgacta
    10201 ataattttcc ttttactaga agcattcatt tcttatgctc ctttctctcg aggtatttat
    10261 taaactcatt tttcatatcc tgatctgaaa gatgaacata ggtatttaac gttgtctgaa
    10321 catgtgcgtg acctaatctc ttttgaacga acgcaacatc ccatccttcc ctaattagct
    10381 gcgttgcgtg agtgtggcga agcatatgtg atgtaaattc tattccagtc cttttaacta
    10441 ttcttctaac tagatcaaga acactttggt actttagtgg tttcccaaaa tagccttctt
    10501 ttaaggaaat aaaaacataa tcatgctcca attcctcact atactcatat atcaagtaat
    10561 ctgtataaag tgacataagt tctttactca catgtattgt tctttccttc cttaatttaa
    10621 tataagcttc attaacatta acatctctag gtgttaaatg gatttgattg tcccaagtga
    10681 caatatcttc aagcctaagc gataacactt caccgattct taaaccaccc tcatacataa
    10741 gcattaaaat taatttatct cttttcgtat gacaagcatc aataatttgc ttaacttcct
    10801 ttgatctcaa tgttcttatc tgtttctttt taacccttaa ctttaagaca ttcttttggt
    10861 atctaccctt attaacatga tgtaaaaatc ctttgaaatt tcttcccttt gcttgtttaa
    10921 atacatcaat tgatttaaat tctcctaatc tacttaaata atcaagaaaa ctcataacta
    10981 catttaaaat tgtattcact gtcgtttctt ctcttatggc tttttttgac tgaagatcaa
    11041 ttacatttga tgctgaagga tatctcaacc aacctacgaa gtctgctaac aactcaaagt
    11101 taatatcatt aagaataaca cctctctgtt ccatgaactc gtacagcaac tttaaatgat
    11161 agcagtatgc cttaatggta ttaggagact taccagtatt atctaagtat ttaataaatt
    11221 tcattactgg ttctattagc tggtattctt tatctagtaa taaatacaat ggatacggct
    11281 tattctccac ttctatcctt tgaaccttca catgttccca cctctttaaa tactttaact
    11341 actacaaatt ataaaccttg ttaattttta tttaaagtaa aatatccctt taatatctcc
    11401 tcttaaacta ctttaactac tattatttat tatactatgg ttaatacatc accggatgga
    11461 tgattatgaa ctgcgatgat tgcattggca ttttctctca ccgcaatact aaaaatttca
    11521 cgtggatgta caatcgaact atttaatgta cctttaaaaa cacaggtttc tttaatcact
    11581 acattttttg aatttaacaa taaaatgaca aaatgttctt gtgttaaatc tttcattgtt
    11641 ggaatcatat aatcagcaac atcacttggt tgcgttattt ttatacgatt attttcagct
    11701 cttctcccca tcctttcccc taactcaaat gctgctttta aagtaattgc tttttgtaat
    11761 ccaatccctt taacttttat caaatcgtta attgaagatt ttttcaattc attcagattc
    11821 gaagcagatt taagcagttc attactaatg tctatgctcg agaatccttt tcttccggtg
    11881 ttaattaata tagctaataa ttctgtattc gaaagacttt ttgcaccatg gcttaacaaa
    11941 cgttctcttg gcatttctga agttaccatt tctttaattt tcaaaaatat acgcctccta
    12001 aaaattgatg gatatcatta taaaaaagtg aattgataaa aaaggaaata aatataaatg
    12061 gaacaagggg taatagttta atcggcttaa atatcatggt aattaaagca actaaaccag
    12121 caatgacaaa tgtaaataaa atgacataaa tagtgaattg gagagggaaa aacaaagaaa
    12181 gtgcagatat tagtaaaacg tcaccataac caatatatgc ccgaaataaa aagtagaata
    12241 tatgcgtggt catactaata atgataaaag ctactggata aatcatactt aacgagagag
    12301 aaacgataca ataaattata attaagcgac aatctaacat taaagaagtg atatcggtca
    12361 tagtaaaaat aagcagaaaa acatatgtag ttataaatag cgtagcattt acgtatgtga
    12421 aatcatactt aataaagacg ataggtatta aagcaaaggt ttcccctaag aaatgtgtta
    12481 gggaaatacg ctttcgacag tttcgacatc gcccttttaa taataaaaaa ctaataatcg
    12541 gcattaattc ataccatttg agtgacgaat tacaataatc acatttcgat cttctatgta
    12601 aataatcaaa tgacgtttct tctatagata taaattgata tagaaaacta aaaatacaac
    12661 tgcaactata agataacaat actaccaaat gacaacctcc ttatgtaaat tatagttagt
    12721 tattaccaaa atgtaaatat acactatttt tcaagaattg aaccgctttt tcatttaaat
    12781 ttttcaatat tgctaagcat aattgatgga tactttaaca acccattact gctcggcaaa
    12841 attaataatg gcaagaaatt gaaccttata aacacatacg atttagagca taaaaaataa
    12901 ccatgaagct ctacctattg attaaataga ttcttcatgg ctattttagt tttagtttta
    12961 taatgcttca aagtctaatt ttgatttaac ttcacttatg aaatacagac taccggtaat
    13021 tactaatgta tcaccttgat aattttttat aaattcaacg tagtcatcta ctaattgtat
    13081 ttcatcattt tcaatactac ctacaatttc ttctttgcgt aacgctttcg gaaaatcaaa
    13141 ttcagttgca taaaacgtat gcgcaattaa acttaaatgt ttgaccatct cgttaatcgg
    13201 ttttccgttt attgctgaga acaaaatatc tactttttct ttatcatggt actgtttaat
    13261 tgtatcaatt agagcatcta tactctctga attatgtgcg ccatccaaaa tgattaaagg
    13321 tttgtcatgc acctgctcaa tacgtccagt ccaacgaact gattcaatac cgtctatcat
    13381 cttattgaaa tctaattcaa ttaatccttg ttcatttaat tcaataagag ctgttatggc
    13441 taatgcagca ttttgtttct gatgttcacc taacatgctt aaaatgattg tttctaattc
    13501 ataatcttta taacggtaag taaattcatc attttgcgat acaacaacaa tttctctatc
    13561 taattcaatt ggctttgcat gttgttcaat tgcgcgttca cgaacatatt ttaatgcatc
    13621 ttcattttta acagcatata tcactggaac gttaggcttt ataatcgcgc ctttatccct
    13681 agcaatatct agataagtac cacctaaaat atctgtatgg tctagaccga tactagttaa
    13741 gattgataaa accggtgtaa agacatttgt cgaatcgttc tttataccca atccagcctc
    13801 aacaatgaca aaatcaacag gatgtatttc accaaaatat aaaaacatca tcgctgtgat
    13861 tatttcgaat tcagttgcaa cacctaaatc tgtttcacgt tccatcattt cacttactgg
    13921 tttaatacgt gatactaatt ctacaatagc gtcatttgat attggcacac catttagact
    13981 aattcgttca ttaaatgttt caataaacgg cgacgtaaat gtacctactt cataaccatt
    14041 ttcaactaaa gctgttctaa ggtaagcaac tgtagagcct ttaccatttg tgccacctac
    14101 atgaataccc ttaatgttat tttgaggatt attaaattgt gctagcatcc attccatacg
    14161 tttaacacct ggtttgatgc caaatttagt tctttcgtgt atccaataca agctctctag
    14221 gtaattcatt gttactaact cctatgcttt taattgttca attcttgcct tcacaccatc
    14281 atatttttct tgataatctt gttttttacg tttttcttca tttataacct tttcaggtgc
    14341 tttacttaca aagttttcat tagagagctt tttatctact ctatctaatt cgctttgaag
    14401 tttagctaat tctttttcca aacggctgat ttccttatcc atatcaatta gcccttctaa
    14461 tggtaatacc actttacctg caattacaac tgatgtcatt gctttctcag gaatttccac
    14521 gtcagtgcta atatttaagg tactaggatt acagaatttg attaaataat ctttgttttg
    14581 tgataaagtt gtttcaattt ctttatcttt agcttgaatt aaaataggta tttctttaga
    14641 caatggcgta tttacttcta cacgtgattg tcttacagat ttaatgattt caacaagttg
    14701 ttgcattgtt tgtttacttt cttcaaaaat caatgattca cgcacttctg gccatgaagc
    14761 tttaacaatt gtgtcacctt catgtggtaa actttgccat attttctctg ttacaaatgg
    14821 catgaatgga tgtagcattc tcataatatt gtctaaagta taactcaata ctgaacgtgt
    14881 aacttgtttt tgttcttcat cattactatt cattggaatt ttactcattt caatgtacca
    14941 atcacagaaa tcatcccaaa tgaaattata taatgcacgt ccaacttcgc cgaattcata
    15001 tttgtcactt aaatcagtaa ctgttgcaat cgtttcattt aaacgtgtta gaatccattt
    15061 atctgctaat gataagttac cacttaaatc gatatcttca actttaaagt cttcaccgat
    15121 attcattaaa ctgaaacgtg ccccattcca gattttattg ataaagttcc acactgactc
    15181 aactttttca gttgagtatc ttaaatcatg tcctggagat gaacctgttg ctaagaagta
    15241 acgcaagcta tcagcaccgt attcgtcaat aacatccatt ggatccacac cattacctaa
    15301 tgatttactc atcttacgcc cgtcttcagc acgaactaaa ccgtgtaata atacatcatt
    15361 aaatggacga cgatctgtaa attctaagcc ttggaatatc atgcgtgcta cccagaaaaa
    15421 gataatatcg taacctgtaa ctaaggcatt tgttgggtag tatcgtttaa agtcttcact
    15481 ttctaaatca ggccaaccta acgtagagaa aggccataaa gcacttgaga accacgtatc
    15541 taatacatct tcatcttgtt gccaattttc aatatcagtt ggcgcttctt ctccaacata
    15601 tatttcgcct gtttctttat gataccaagc cggaatttga tgaccccacc ataattgtct
    15661 tgaaatcgtc caatctctaa tattttccat ccattggtta aatgtatgtt cgaaacgttg
    15721 cggataaaaa tcaatacgat catctgtttt ttggttatct aatgaacgtt tcgctaagtc
    15781 ttccatgcgc acaaaccatt gtgttgataa atatggttca acaacagcgc cagatcgttc
    15841 tgaatgacct acagaatgaa catgatcttc aatcttgata actaaatctt gttcttttaa
    15901 atctttaact agctgtttac gacaatcaaa acggtccata ccttcatatt tacccgcttt
    15961 gtcgttcatt ttaccatttt catccataac gataatattt tctaattgat gtctttgacc
    16021 aatttcaaaa tcattagggt catgtgctgg tgtcactttc atagcaccag aaccgaagtc
    16081 tatatcaaca tactcatctg ctaaaatagg cagttcgcgt cctacgattg gtaatataac
    16141 agttttaccg attacatctt tgtatcgttc gtcattaggg ttaacaacaa tcgctgtatc
    16201 acctaacatc gtttctggtc ttgttgttgc aatttcaata aaaccttcac catcagcgta
    16261 aggatattta aaatgataaa acgcaccttg aacatcttca tgtattactt caatatcaga
    16321 taaagctgta cgtgctttag gatcccaatt tataatacgt tcgccacgat aaataattcc
    16381 tttattgtat aaatcaacaa aaactttttt aactgcttta cttaaacctt catctaaagt
    16441 aaaacgttct ctactataat ctaaacctag acctaattta gcccattgcg cacgaataaa
    16501 tgacgcatac tcttctttcc aatcccatgc ctgttctaaa aacttttcac gaccaagatc
    16561 atatctagtt attccttgtt catttaattt agcttctacc tttgcctgtg tcgcaatacc
    16621 agcatgatcc atacctggta agtataacgt atcgtatcct tgcatacgtt tcatacgtgt
    16681 aatgatatct tgtaaagtcg tatcccatgc atgtcctaaa tgtaatttac cagttacatt
    16741 tggtggcggg ataacaattg tatatgtttc ttttgattta tcttctgacg gtttaaaata
    16801 accattcttt acccattctt cataacgtcc cgcttcaact tcacgaggat catattttgg
    16861 tttcatttcc atttgctata cctcctaaaa aaataaaaat atccatccta tatacaaata
    16921 ggacggatat tccgtggtac cacctatatt caagaaggat gattaatatc aaattcactc
    16981 ttttaacata attggaataa tcataccaat actatcatcg tgaaatttga aatgcttcat
    17041 ctcttcaagc actctagatt atgattaacg ctcaaacacg tcttagccta ctattaatca
    17101 cgttcagcta agatactctg tgggctacct tcagtaaaaa tcatttacat actcacacca
    17161 aatcatatgc tctctttaaa ataatttgaa cttactcttc ccaaatccta tattaaactc
    17221 ttaacttata gtataatgat tgacaaaata agtcaatgta taggtgggaa taaaatgaat
    17281 gaatgcgcat ttggtactaa agatccagtc tacttagact atcatgatca tgtatgggga
    17341 caaccgctct atgatagcaa ggcattgttt aaacttttag cattagaatc acaacatgct
    17401 gggctatctt ggttaactat tttaaaaaag aaagaagcct atgaagaagc attttatgat
    17461 ttcgaaccag aaaaggtagc acaaatgacc gctcaagata tcgaccgctt aatgactttt
    17521 ccaaatatcg ttcatcatcg taaaaaatta gaagcaattg ttaatcaagc tcaagggtat
    17581 ttaaaaattg aacaagcata tggtagtttt agtaaatttt tatggtcata tgtaaatggt
    17641 aagcctaaag atttgcagta tgaacatgct tctgatcgta tcacagttga tgatactgca
    17701 acacaactat ctaaagattt aaaacaatac gggtttaaat ttttaggtcc agtaacagta
    17761 ttttcgtttt tagaagcagc cggtttatat gatgcacatt tacaagattg tccatcaaag
    17821 cctaaacata attaatttgc tatagcatca taaagtaaac gagacatcat ctatttttaa
    17881 ctaatagact gtctcgtttt ttaatgtgaa aatttattca aatcataagt taaagttcca
    17941 agttaatgct ctgctaatat tagatatctt aatctaaaaa tagatgtaca acctttgctt
    18001 aaaccttaat aatgaattct atcctaagca taattcaact tagatggatg atagtttcta
    18061 atcttcacgc ttattcgaac gatattttaa aaagtaatta attcctggtg caataataaa
    18121 taggatgaaa aagattcgga aaatatgata actcgtaatc atagcaacat cggcaccagt
    18181 agctaatgca actaaaacta tctgattaac ccctcctggt gctgcaccaa gaaacaattc
    18241 attaatagga ttattatcaa agaaatgtat gatataaacc atgattagcg caccaattat
    18301 caacataata ttttgaattg taattgcgat tgctagtcta ccttttaaat ctgacaataa
    18361 atgcgcaatt tgaactccaa ttctaatcat atatattagt tgtgccatgt tcaacaacca
    18421 atgatctagt gtaaatgtta aacctgtaga aaaattccaa acaattaata caatgagtgg
    18481 tgctaataat tgaaatgttg gaaactttat tttagacata attagataaa ctataaagat
    18541 agctatcgct aaaataacta tttgccctat gtttaatact tgtgataaag gcaagacttt
    18601 tgttaacttt ccattcgcat gcatgttacc atcatgaaaa aaatatgaaa tgaacggtac
    18661 taaaacaaca acaaatataa ttcgtgatgt ttgcgttaag ctaacaacta acaaattagc
    18721 acgtttgtct tgttcagcca tgaccagcat ttgtgttagt gctcctggta taacacttaa
    18781 aatagctgtt tctgtattaa tacgtgcaat ttttttaaaa acaaatgcca ttactattgc
    18841 aattaataat atcgaaatag atacaacaat aatcgaaagc caattgtttt taatatccat
    18901 aacgacattt ttcgtaaacg ttgatccgat ttgcacacct aatagtacaa tacctaattc
    18961 actaagtaag aatggccatt taatatcaag tttgaaaact tttacacaaa tgattgatgc
    19021 gataatagga ccaaacataa atggaagtaa tacgtgcgat gaatacagta gaatactaat
    19081 aaaaaatgat aaaacgaaca caatgaaatt atttctatat atcattgcca tgttttccac
    19141 ttctttcaat aaaaaataaa atgactaaat tgctgcttga gcttcacgtt tgttaagata
    19201 acaatatccg ctagcagttt tgactacaaa gcatatatgg actttcacta tcaagtcgcc
    19261 gcccatgcct tatatacatt taaaaagagc ctgaacaaag ttcaggctct caatttgtcc
    19321 gtatatttat tttacaatac gacttaaagc cgtatcaaat gcttgaatcg ttttttcaat
    19381 atcttctttc gtgtgtgccg tagataagaa tgtaccttca aattgagatg gtggtaaaaa
    19441 cacaccttct tttgccattt ctcgatacat ttctgcaaat aatttcaaat cacttttatt
    19501 cgcttgttca aaattagtta caggtccttc atttaagaaa taaccaatca ttgaacctgc
    19561 tctatttaca gttattggta cattgtgttt agcaaataca cgttttaaac cgtcttcaag
    19621 tatatcgcct aacatattaa aatactcata tgtctctggc gttaattggc ttaacgtttc
    19681 ataaccactt gtcattgcaa gaggatttcc tgataacgta cccgcttgat aaatatttcc
    19741 taatggtgct atatgatcca tgatttcttt tttaccacca aatgcaccta caggtagtcc
    19801 tccaccgata acttttccta agcaagttaa atctggtgtc acaccaaagt aaccttgtgc
    19861 acaatgataa ccgactctga aaccagtcat tacttcatcg aaaattagca atgcgccgta
    19921 ttcagtcgta atatctctta atccctgtaa aaaaccttca atcggcggta cgacacccat
    19981 attaccagca acaggttcta cgattacacc agcaatatcg tttccaaatt tttcgaaagc
    20041 gattttaagt gcatctaaat cattgtatgg aactgtaatt gtatttttag caataccttc
    20101 aggcacacca ggagaatccg gcaatcctaa tgttgccacc ccagaaccag ctttgattaa
    20161 taacgaatca ctatgaccat gatagcaacc ttcaaatttc acaattttat ttctgccagt
    20221 ataaccacgt gctaatctta aagtatccaa tgtagcttct gtaccagatg acaccatacg
    20281 cactttttct attgaaggta ctcggtcaat aacgagctgc gccaatttat tttcaagtaa
    20341 tgttgatgca ccaaaacttg tacctttatc aattgcttca tgtaaatgac taataacttg
    20401 agggtctcta tgtcctaaaa taagtggtcc ccaacttagt acatagtcga tatactcgtt
    20461 accatcgata tcataaattt ttgaaccttt accgtgatcc ataaaaattg ctggtgtatc
    20521 tactgattta aatgcgcgta ctggactatt tacaccacca ggcattaaag tttcagcaac
    20581 cttcattgct tcttctgatt tcgtatatct cataaagtaa attcctccta cagtttatgg
    20641 aaaatcataa atataaaacc ttatttatct aaatagcgac aaatgtcctt tgcaaaatac
    20701 gtaataatca tatcagcacc tgcacgtttc attgaaacca tttgttccat aacgacacgt
    20761 tcttcatcta tccaaccatt ttgtgccgct gctttagtca tactatattc tccactcaca
    20821 ttatatgcaa caactggaac attcgtatga tttttaacat ctcgaactat atctaaataa
    20881 cttagagcag gtttaacaat catcatgtcg cacccttctt taagatcact ttctaattca
    20941 cgaagtgctt ccaaacggtt agcagggtcc atctgatacg tttttctatc cccaaatgat
    21001 ggcgctgaat ctgctgcatc tctaaaaggt ccaaagaaac ttgatgcata cttgacacca
    21061 taactcatta taggaatatt gtaatagccg gcttcatcta atccacgacg aatttcagca
    21121 acaaaaccat ccatcatatt acttggcgca ataatatcag caccagcttc cacttgagaa
    21181 attgctgttt taacaagtag tggcaatgat ttatcattgt caacgtcatg tgtatggtca
    21241 tcaatcacgc cacaatgacc atgatcagta tattcacata aacaagtgtc tgcaacaatt
    21301 aataagtcat catacatttt tttagcaata cgtgttgcct gttgaataac accatcgtga
    21361 atgtatgcac cagtacctat atcatctttt gagtttggaa caccgaaaaa cataatggca
    21421 cgtatgccta agtcataagc ttcttttaat tcactttcaa gtaaattcaa actgatttgg
    21481 tatacacctg gcaatgactt aatttctttt ttcacatcgt ctttttcaac tacaaaaatt
    21541 ggatatatta aatcttcttt tcttacatga ttctctctaa ccatatctct cattgtcgct
    21601 gatgatctca atcttctatg tctatcaaat ttcattttta gcccctactt tctaaaatct
    21661 tttcaattag tgattcgagt gtttgaattt ctgcaattgt tactggttgt tgatatgatt
    21721 taatggtccg tgctgtttgt tctccaatag caaaatacga cttgaatttt ggtacaaatc
    21781 cttcattaaa ataataacgt actgccgacg aacttgaaaa tgttaatgca tcgatttgtt
    21841 gatgttctat catttcttta acatcttgta tattttgttt gttaggcact gaagtatata
    21901 aatctatttt aacaacttca ttatctttag ataacgctgc taataacaat ggtctcgcca
    21961 attcactcga aggcaaaagt attttttggt tagtttgatt aaatgatttt aaaaatcctt
    22021 cttgagaaaa gtcgtttggc ataaaatcaa ctcgaatgcc aagtgattca caatattgcg
    22081 ctgtcttact tcctatcaca gcaatgttat caacattaat tccttttaga tatttataaa
    22141 agaatttcac agcattttta gatgaaaaaa taagccagtc atagcgttga tttaacaaat
    22201 gaatatcaaa atttagtggc tttatatcaa taaagggttt gtgaataatt gatactaaat
    22261 cactttgcat gtcatttgtt tgtgtcataa ctacaactgg cttcatattt aaacgtcact
    22321 ccattattta atgttgttca tttaagcgtt ttataatttc ataagcacct tgctctttta
    22381 atttgttact cactgttttg cctaactcaa ccggatctgt tccgttcatt gtatattcaa
    22441 atcgttcttt accatctggg gtcataatta aacctgtaaa ttcgatttcg ttttgatctg
    22501 agattgtagc atatcctgca attggcacct gacaactacc atccatttct gctaaaaacg
    22561 ttcgttcagc agtcacacat tttgcaacct catcattatg tactttgctt aataatgtta
    22621 atagttcttc atcgtcacta cgacattcta tccctaaagc accttgtccg attgcaggta
    22681 acaatgtatc tctatcaaga taagatgtta caatatcatc tgaccagccc attcttctta
    22741 aaccagctgc agctaaaata atcgcatcat aatcttcagt ttgtaacttt tctaatcgtg
    22801 tatctatatt acctctaatc catttaatct ctaaattagg atacttagat aatatttgtg
    22861 caccacgacg taatgaacta gtaccaataa tactgccttc tggcaattgg gatagtggtg
    22921 tatgtgtttt agaaatatac gcatcaaaag gtaattctct atcagggata caacctaatg
    22981 ttaaaccttc cggaattaca cttggtacgt ctttaagcga gtgtattgcc atatcgatat
    23041 ttttttcaaa aagttcatgt tgtatttctt taacaaataa gcctttgcct ccgactttag
    23101 acaattgttt atctactata cgatcgcctt tcgtgacaat ttctttaatt tcaatttcta
    23161 gatttggctc gacagctttt aatttatcaa taaattgctg gctttgtgtt aaagctaatt
    23221 tacttcttct ggagccaacg actaatttac gcatgttcaa ttcctcctag gaacggattg
    23281 ctctagatta ttttctcaat tcacaaaatg tgttgcaaaa aataaattaa tcatatttaa
    23341 gcaaaataaa ataatgttat agtatattaa atatcttgaa ttcaaccatt tgttgattct
    23401 aagtaaaata taacttccat ataatactgt aataattgaa gagagtatta ccttcgggtc
    23461 aatgaatata cgttcaccaa ctgaaattac accccactgt gtacctaaaa taatactaaa
    23521 tatgagaatt atccacccac ttaacgttga gtaaaacaca attgattcaa gtgtagcaac
    23581 gctaccaatt ctaaagtatt tttgatcaaa acgtttttcc ttcaaattac ggtattgcat
    23641 gatatacagt aatgcattga caaaagctaa ggcaaagaag acataactta acacagctag
    23701 accgatatgg actaacagta actcgtctac aacagcaatt ttctgaacct tattagtata
    23761 atgtgtcggt tgaaatgtat tcatccctaa aagtgttaac cctattaaat tcaagaaaaa
    23821 cacagagaaa ttcaaaactt tgataaggtt aagaattaaa gagattgaaa taatcagcca
    23881 acttaaagta tagaatacat ctgaaataga ccctaatgga atatgtctag tttgtataat
    23941 aaaaatagat aaagagattg tttgtaaaac ccaaacaatc cccaataaat atatgcctaa
    24001 acttctaatc ttatgacttt tttgtacaaa atcataaaaa tagcaaatga tactgattaa
    24061 gtatattaat aatataattt cattgaatcg aataaacagg ttttcttgca tattatcacc
    24121 atattaatat gcttattcaa aactaaagat acgtcgcgct gaaatttcct tgaccttgct
    24181 ttctttttgt tgcttcgctt gctcatgagg acattcagct tcgatatcaa atatattttg
    24241 aaatagctct aatttttcat tacttttctt atcactactt aattctttgg cctgtttaat
    24301 aggatctttc aacatttgat tgatgatact ttttgtatgt ttagatataa ttttacgttc
    24361 tctttcgctt aaccctggca atttacgatc aatactgtcc atcgtttctg cttgaattgc
    24421 catagctttt tcacgtaaag ctctaatcac tggaacaaca cccaacatac taatccactc
    24481 attgtgtgca tgtatttctg caggaatttg ttccgaaatt gttgcagccg ctaattgtcg
    24541 ctcacgtaag tttgcatcaa ctaaaccttt taagtcatca acatcataat taaagatgtt
    24601 tgtgatggca ctaataccag gttcaatatc tcgaggaact gcaatatcaa tcaatactag
    24661 tgaatcttgc tttctatttt ctgcaattct ttctatcatt tcatttgtaa tgatataaga
    24721 ttgtgcactc gttgaactaa tcacaatatc tgcactttca agtaaatttg gtaatgatga
    24781 tagttcatca tatttcactt gatgctttgc tgctaatttc atagcatttt caattgttct
    24841 atttactact gtaatatcag taattccaga accaagaaga tttaatagtg ataattcact
    24901 catttcccct gcaccaataa tgatagcttg cttacttttc aatttgccaa atactttttt
    24961 cgccaactcg accgcagcat aagacacact tacagcatta tcagctatat ctgtttcatt
    25021 atgtgctctt tttgcaaaag taattgcctg tttaaataga tgattaaaaa ttgttcctgt
    25081 cgtacctgtg ctttgcgcta agaaaaatgc atctcttatt tgacctaaaa tttgagtttc
    25141 tccaagtacg attgaatcta aaccagaagt gacacgcaat aaatgttcta ctgcttcgtc
    25201 ccccactttt acttctgaca ttgctttaat atcatctact tcaaatccaa atgcacgagc
    25261 tagaaatcgt tgaatatagt aacgacctgt gtgaatttga tcaacaacag catatacttc
    25321 agttcgatta catgttgata atatgacatt ttctaaaata gatttagttt catataaatc
    25381 ttcatgggca attcgtaagg catcatctct aaaagcaact tgctctctta gtgcaacatc
    25441 agctgtgcga tgatttatac taattgcaat aaaatgcatt tgaaacgccc ccatatatac
    25501 taacaataca aatattataa cataatttaa aatccaatta agtacgatgc ttaaataaat
    25561 tataatcttt ctaatttact tttgagtttt tgacaaaatg atgaacgttg aaaaatgtta
    25621 tcaattgtgt actaattaaa agtttcgtgc ttacattatt tttaaaattt ttgtataagt
    25681 tttgacgtac aaaaactatg aaatatacgg ttcaattaaa ttccatattt gttgttgttt
    25741 attattttga attgatgaat aacttacaat tgtatcgtct gggtccatat ctaattgtgt
    25801 cttaatattt ttaatatgct tttgaacctt accttttgga attttgtctt ctttagtgca
    25861 tataactaaa gtaggaatat caaaatgttt caaataattg tacattaaga tatcatcttg
    25921 tgttggatca tgtcttaaat caactaattg aataactaat tgcaaattct ctctcttagt
    25981 tatatattcc tcaatcattt tcccaaattt ttcacgttgt gttttactta ctttagcata
    26041 tccataccct ggaacatcca caaaaataag ttgttcatct atattataaa aatttaacgt
    26101 ttgcgttttg ccgggttgct gtgatgtacg tgccatattt tttctgccaa tcatactatt
    26161 gataaatgta gacttaccta cattagatcg accgctcagt gcaacttcag acaattctgt
    26221 ttctggatat tgttcttctt ttactgcact aatgattaat tctatattat taggattaac
    26281 tttcatttta tatcctcact tttttatatt ctctttcaat tctaccatta tataatattt
    26341 gaacctttcg gtatacatta taaaaaagct ccgatcaaag ttaaactatt tgagcgtcaa
    26401 ctttgattgg agcttttcac ttttataaca catcaatgat taagctgatg ttttactatt
    26461 attaattaaa ttgccttctg cgtcgtatag ttctggttca gtttcttcat taattgtttg
    26521 tgctgtaata actaccttcg ttacattttc gttagaaggc acatcaaaca taatatcgat
    26581 taacgattct tctatgattg aacgtaaacc acgcgcacct gtttttcttt caattgcttt
    26641 ttcactaatt gctgataaag cttcttcagt gaactctaaa tccacatcat ctaattccag
    26701 cattttagta tattgtttca caagtgcatt tttaggttgc gttaagatgt ttttcaacgc
    26761 agttacatct aatgtttcta aattagctac aattggcaca cgtccgataa attcaggaat
    26821 caaaccatag gcttgcaaat cttctgggcg aatttgtgct aataatgctt gttcgtcata
    26881 tttatcagct tcattgcttg agaaaccaat aactttttca ccaagacggc gcttaatcac
    26941 ttcttcaata ccatcaaagg caccaccaag aataaataag atatttgttg tatcaatttg
    27001 aatcatttct tggtttggat gtttgcgtcc accttgtggc ggaacacttg cagtcgtacc
    27061 ttctaagatt ttaagcaatg cttgttgaac accttcacct gaaacgtcac gtgttataga
    27121 tgtgttttca gatttacgtg caattttatc aatttcatct acataaataa tacctttttc
    27181 ggctttatca atgtcaaagt cagctgcttg aattaatctc aacaagatat tttcaacatc
    27241 atcgcctaca taaccagctt cagttaaact tgtcgcatct gcaattgcaa atggtacatt
    27301 caacgtcttg gctaaggttt gagctaataa tgttttacca ctacctgttg gcccaattaa
    27361 tgcaatgtta cttttttgta attcaacatc atcttctttt ggtcctaatt gttgaatacg
    27421 cttatagtgg ttataaacag ctacagctaa agatttttta gctttttctt gaccaataac
    27481 atattcgttt aaatgatcca taatttcttt aggagtaggt aattctgtca tcgcttcaga
    27541 agtgttttga gctaattctt cttcgacgat ttctgagcat aattcaatac actcattaca
    27601 aatatataca ccacttcctg ctacaagttt ttttacttga tcttggtctt ttccgcagaa
    27661 agagcatttc aaattttctt catcttcatt gaatttaaac attcttttta cacccctatt
    27721 cgttaaagac tatactagat tggatgttac aatgcaacat attaacatac aaactttttg
    27781 cttaaagaat agtagcagat acataagcta tgcatctgct actcattttt gtaatttaat
    27841 atttaatgaa gattaatctt ctttagttcc ttcaacgaac tttgcgttat ctcttaataa
    27901 atcgataact ttttggatac gaacatcatt tttaatgata tcagtattac ctaaagtatt
    27961 tttgatatct tcaactgaga tattaaattg tttactcatt ttttctaatt ctttatcgat
    28021 atcttcatca gtagcttcga ttttttcagc ttcagcgatc gcagttaaag ttaagttagt
    28081 tttaacacgt tgttctgcat cgtctttcat ttgctctctt aattgagttt catcttgacc
    28141 tgagatttgg aagtacgttt gtaaatctaa accttgttgt tgaattcttt gtgcaaattc
    28201 agacaccata cgatctaatt cagtattaac cattgcttca ggaatatcga ttgttgtatt
    28261 atcagtagct tttgtaatcg cttcttcttt ttcaacattt tcagcatctg tagctttttg
    28321 ttcagctaaa cgtttacgta agttttcttt gtactcgtct actgtatttg cttctgcatc
    28381 taattcatta gcaatttcat ctgttaattc tgggacttct ttaaatttaa tttcgttaac
    28441 ttttgttttg aaagttgctt ctttaccggc taattcttca gcatggtatt cttctgggaa
    28501 tgttacgaca acatcttttt cttcgtcaac tttcatacct tctaattgct cttcgaaacc
    28561 aggtatgaat gaacctgaac cgatttctaa atcgtaacct tcagcttgtc caccttcgaa
    28621 ttcttctccg tcaactgaac cactaaagtc gatgttaact gtgtcgccat tttcaacaac
    28681 accatcttct ttaacgacca tttcagctaa atgtcctaag ctgtggtcaa tcgcttcttg
    28741 taactcatca tcagataatt cagtttcttg tttttcaatt tcaagacctt tatagtctcc
    28801 taatttaact tctggctcaa ctgtaactgt tgcttcaaaa atgaaatctt tacctttttc
    28861 aatttgagta acacttactt ctggttgtgc aactggttta atatcagttt cgtcaattgc
    28921 ttcaccataa gcatctggta ataaaatgtc gatagcatct tgatataatg cttctacacc
    28981 aaagcgttgt tcaaaaattg gacgtggcac tttaccttta cggaatccag gtacgttaat
    29041 ttgtttaacc acttttttga atgcttgatc taacgctttg tttacttttt ctgcaggaac
    29101 agtaacagtt aataaacctt cgttaccttc ctttttttcc caagttgctg tcatgtatat
    29161 atacctccat gattaactaa tttatttttt caacttccct attatatcat aggtctattc
    29221 cctatacaaa cattgaaatc acaacgttta tatatttgta aatcaacttt tttcgtcaaa
    29281 actatattaa aatgctgagt attattttgc aacaatttaa ctattattat tcaaatctaa
    29341 ttgctgaata aaattaaatg catcgatact ttgtaattca catttaagtc ctaacatatt
    29401 tttgaaataa cattcgtatg cattaatcca tttattcgtt tcaaacaaag tttcaatatc
    29461 aattggataa atcatgatag agtgattatt cataatatgg tgtgcttctt cagcgatatg
    29521 taaagcacca tcatttaatg tttccataac gttaggtata actttttctt taagtgttgt
    29581 atgttctagc ccttttaaat tagctggtac aaaagttaca tccataccat acttttcaat
    29641 tgtcagttct tgtgtacaat ttgcgaacct taaatactca atcattaaac taatgaggtt
    29701 ataactgtac gtattagatt ttaatatata taataccgtt tcttgaaatt gaaaatgacc
    29761 attgtctatt aacttcaaaa tcaagtgcgt ctgttccctc attgataacg tatcaaaatc
    29821 agctaatgac tgagtcaatc gtttttcatc ttcaattaat tttgacttag caaattcttt
    29881 aagaggatgt aatgccattc ttgttttgtg atctttgact tcatcaataa tttgatgaat
    29941 tacttctacc gcttcaaaat attgtcccaa accaatcaaa cttttcacgt aataaatcat
    30001 caatgcatca tattgttgta tgccagtttt caataagaca attgtttctt ctcttaattc
    30061 aagaaatgac cccgtctcat ataacatacg gcatttcatc attgctattt cttcagtgag
    30121 ttcaaattga cgttcatatt gcacaatata ttcatacatc aggtcatagt tttggtcttg
    30181 ttcagcacgt tttatatctt tatataactt tttcgaagag ttaggaaact gaataatatc
    30241 tgacatatat gtcacctcgt ttgattataa atcttgaagg attaatgcac tccacgtttc
    30301 gaaatcatca tatgactcta tgttgccttt ttcaacccat tttattcgta aagtatctgt
    30361 ttctttagct tctacatcat tcgtacttac agtgatttta aatacaacac caatatgtac
    30421 cttgcccact tcattattat cgtcattaat aaaaccgata tattccatat tttgtgaatc
    30481 ttgctcactt aaacctactt cttcttctaa ttctctctgt gcattaactc tcaatacttc
    30541 gttaatagat tctgttcctg gaacatcatt catatgaccg cctacaccta ttgaagattg
    30601 tccatgcaat cgagcttctc caccgccaga taatcgttca tacactaata tctcgccatg
    30661 ctcattttca agtaaacaat aagaaatgag ttgtttatat gatggatcct cttccatatc
    30721 gccgcgtcgc ttaacttcat attgacttag cgcatcaaaa atattttgac cttctggttt
    30781 attcttattt aaaaacccat tgaaagtatt cttttcatta ttaaaaataa tttctctagg
    30841 tactacaatg atttgttcat caaatttaga catcgtataa aactcctcac tttaatgttt
    30901 gttctcttaa atgcatttta gcaaatatgt catattttag cgattaaatg tggcattttg
    30961 ctatttaaaa aataaaaaag agatgacaat gccatctctt ttttataagt gttattattt
    31021 taaagcatct ttagctttag ttactaattg agcaaatgct ttttcgtcag aaattgcgat
    31081 ttctgataac attttacggt taatgtcgat accagctttt ttcaaaccgt tcattaaacg
    31141 tgagtagctc atttcatgtt gacgagctgc tgcgttgata cgtgtaatcc ataatttacg
    31201 gaagtcacgt ttacgttgac gacggtcacg gaaagcatat tgacctgatt tcattacttg
    31261 ttgcttagct actttgtata atgtatgttt tgaaccgaag taacctttag ctaatttaat
    31321 cgttttttta cgacgcgctc ttgttactgt tccaccttta actcgtggca taaaaaattc
    31381 ctccttaggt aattcctacc gatttctgta tttgttctta ttttttgtat gctaataatt
    31441 gttttacacg tttcatatcg ctcttagaca ctaatctagc tttacgtaat tgatgttttt
    31501 gtttagtgct cttgtttgcg aataagtgag atgtgaaagc tcttgaacgt tttaattgac
    31561 ctgaagcagt tcttttaaca cgtttagctg ctccgcggtg agttttcatt tttggcataa
    31621 tataagtcct cctgtaatag ctttaattat ttttcagctg ttggcgctaa catgataaac
    31681 atttgacgcc cgtccatttt aggtttttgt tcaactgttg ctatatcttt gcattcatct
    31741 gcatattttt ctagcacacg ttgaccaatt tccttatgcg taatggcacg ccctctgaaa
    31801 cgaatagata ctttacattt atcgccttta gttaagaatt tacgtccgtt tttcaacttc
    31861 gtttggaaat catgttcctc aattgttgga cttaaacgaa tttctttaac attgataatt
    31921 ttttgtttct ttttcatttc tttttctttt ttctgttgtt cgaatttgaa tttaccgtaa
    31981 tccataattc ttgcaactgg tggtttcgca ttcggtgcaa cgaccactaa gtctaaatct
    32041 acacgttcag ccatttctaa agcttcacgc tttgatttaa caccaatttg ttcaccatct
    32101 tgaccgatta aacgtaattc ttttgcacga attttgtcat tgatttgagt ttgatctttt
    32161 gctatggttg acacctccaa aatttttacg aaatttgcac caagcaaaaa ggaagagcag
    32221 gtataaaata cccgctcttc cttatacaca gttatgtgta atgtgattaa cctgccaact
    32281 gctttatgcg tcgctacagg tgagaagcgg gtgcttctac ttggttcgtt tcgtattcaa
    32341 cgttattaat catatcaaca attcacattt aagtcaacac tataactgta attattttta
    32401 ttttaacctt ttatttcatc cattgacacg tcttgacgta aatctacttg ttctaatgga
    32461 atttttttcg ttttatatcg aagcttatga taaataaaga atgctaaaaa tactggaatt
    32521 cccatatacg taattaagaa gcgactaaaa ttaaaatctc ctgttttaat aaagtcaaca
    32581 tcttgaccaa taattactac aatacataaa aagccagcaa ataatggtcc gaatgggaat
    32641 aatttagcag tatattttaa tttagatttg tcataatttt gtttatcaaa tgctcttcta
    32701 aatcgataat gacttactgc tatacctacc caagcaataa aaccagttaa accacttgct
    32761 gcaacgatat attcgtatgc accttttgta aggctttgta atacgaaaat tatcactaca
    32821 atgattgctg taacgagtaa cgacatatat ggcacaccgt ttttatttgt tttaccaaat
    32881 gcttcaaacg ctaatttatc tttactcatt gaatatagca ttcgagttga agcatacata
    32941 cctgagttac ctgctgataa cacagacgtt aaaatgactg cattcataaa tgatgctgca
    33001 aacgcaaatc cagcattttt aaacactaat gtgaatggag acgttgctac attatcacta
    33061 ccccccatta atgcactact atcataagga attaacatac cgataacaaa aatggctaaa
    33121 atgtaaaata ataaaattct ccagaatact tgtttaattg ctttcggcac agcacgttca
    33181 ggattttctg attcaccagc cgtaatacca attaactcag taccttggaa tgagaaacca
    33241 gcgattaaga atacacctag aattgataac aaacttcctc ctaagttgcc accaagaatt
    33301 ggaccttcac ctttattaaa tatttcgaat cctacaacat gaccacccat gattccgaca
    33361 atcgttaata aaccaattgc aatgaaaaca ataactgtaa ccacttttat caatgccaac
    33421 cagtattcac tttcaccata gacgcgaact gataacgaat tcagactaaa aattataact
    33481 aagaacaacg cactccatgc ccaagcgggt atgccttgca atggtgtcca atattgaatg
    33541 acttgtgctg caatcgtaat atctgctgct acagtcacta cccagttaaa ccaatagttc
    33601 caaccaagcg caaaccctaa agatggatca acaaatcttg tagcatatgt actaaatgaa
    33661 cctgatactg gcaaatacgt agccatttcg ccaagtgacg tcattaagaa aaataccatt
    33721 attccgataa ttgcgtatcc tattaatgca cccaaagcac ctgcatcatg aattgctcca
    33781 ccagaagtta caaataaacc tgtaccaata caacccccaa tcgcaatcat agaaatatga
    33841 cgatctttaa gtcccctttt gacaacattg ttactttcat tttgaacttt tgacatataa
    33901 gctccctttc catgttttag aggccaagaa ataaataacc tttattcact ttcataccct
    33961 tttttacaga acatagtgat attaaaagta aaaaagtggc tacatacctt taagatatgc
    34021 aaccactttg gcaattatct atataaggta gcacatcaca ttctgtgaca gtacagttcc
    34081 tattcgaaaa ctgtcccaat agatataatt tatggtttat atctatttcg gcatctttac
    34141 ctttcacttg ttcaacttat gtaccataaa tacttctgac aagttactaa ttaaacatgc
    34201 aacctctaac tcaatttaat attttaacta acttgtaata tacaggattc atcacgcata
    34261 atcaaccctg taaaacttga tacgcaataa aagttttaaa gcattttatt gcgacaactg
    34321 tctatctatg ttttttcaaa cgaatttcat caactagatt ccagataaat tcatcttttt
    34381 caactgtttc ttggtcttgc gatccatatt gacgcacatt cacttgatta ttttcaactt
    34441 ccttatcccc aactacgatt tgataaggta ttttttgcat ttgagcttct ctgattttat
    34501 aacccatttt ttcattacgg tcatcaatac ttacacgaac gccttgagat ttcaattcat
    34561 cttgtaattg gcgcgcataa tcataatgta aatcaacgtt aactggaatg atttgaactt
    34621 gttttggcgc taaccaagtt gggaatgcac ctttagtttc ttcagttaaa aatgctacaa
    34681 atcgttccat tgttgataca acaccacgat gaataacaac tggacgatga tgttcaccat
    34741 cttgaccaat ataagttaaa tcaaaacgtt ctggtaataa gaaatcaagt tgtgctgttg
    34801 ataatgtctc ttctttaccc atcgctgttt taacttgaac atctagtttc ggaccataga
    34861 atgccgcttc accaatcgct tcttcgtacg ataagccaag ctcatccgct gcctctttaa
    34921 gcatattttc agctttattc cacatatcat catcatcaaa gtacttttct ttatcttcag
    34981 ggtctctata acttaatcta aagctataat cctcgaaacc aaagtcttta tacacatcaa
    35041 taatcatgtt tacaacgcgt ttgaattctt ctttaatttg atcaggtcga acaaagatat
    35101 gtgaatcatt taaagtcata ccacgaacac gttgtaatcc tgatacagca ccacttgctt
    35161 catatctatg catcgttcct agctcagcga tacggatagg taattcacga tatgaatgtg
    35221 gtttattcgc ataaatcatc atatgatgtg gacagttcat tggacgtaat accatagatt
    35281 cagtttcatc taactgcatt ggtgggaaca tatcttcttg atagtgatcc cagtgaccag
    35341 atgttttgta taaatcaaca ttagcaagta ctggtgtata aacgtggtca tatcccatgc
    35401 taacttcttt atcaacaatg taacgttcaa tttcacgtct aattgttgca ccgttaggta
    35461 accataatgg caaaccagca ccaactaatt ggctatttgt gaatagttct aactctttac
    35521 caattttacg atgatcacgt tctttacgct cttctaacat ttgtaaatgt gctttcaatt
    35581 cttttttatc aaagaaagca gtaccgtata tacgttgtaa cattttgttg ttactatctc
    35641 cacgccagta tgcacctgct gtagataata gtttaaactc tttaatttta gctgttgatg
    35701 gaacgtgaac tccacgacat aaatcagtaa aatcaccttg actatataat gttacatttt
    35761 catcttcagg aatcgcgtcg attaattcta atttgtattc atcattgctg aataactctt
    35821 tcgcttcatc tcgtgaaacc acttttcgtt cgattttcat attttcgtta acgatttgtt
    35881 tcattgtttt ttcaatttgt tcaaagtcat cagatgagat gttttggtca atgtcgaagt
    35941 catagtagaa tccaccttct attacaggac ctacaccaaa tttaacatta ccatataacc
    36001 ttttaatcgc gtgtgccatt aaatgtgcag tagaatgacg taatacctct aacgcttctt
    36061 cactacctgg tgtcacaatt tcaattgatc catcagtttc aagcggttta gttaaatcta
    36121 caagttgccc gttaaatttg ccggcaacag cttttttacg taatccagga ctaattgatt
    36181 gtgctatatc ttcagtagta gtacctttat caaacgcctt tttattacca tctggaaatt
    36241 gaatattaat ttgttccatg ttaaaccctc ctatttgctt tataaatact tttatctgac
    36301 ctcgttaaaa aattgaacaa caatcatcgt tttaacatta aacctattaa caataaacag
    36361 ggaatttatg acaccaacaa acatttatgt ctgaaaaaaa caaaaaatcc cgcccctata
    36421 caagggacga gatcgtcgtg gtaccaccct agttatctaa tacaatttat gtattaaata
    36481 tctctgctta agataacggt cttgatccgg gtattcatta caaataccat aaatgaagta
    36541 gtaatcatct aatttattaa ccatattctc atcaaacaat ggctttctgt gtaatattga
    36601 ttagatcatc ttgtcttcaa tcatttaaac gatttagatt tgtattcatt atacaccaat
    36661 cattttaaaa ttcaattgtt tctgaaattt tctcctgata aaaagtatgg tgttgacaaa
    36721 gatttgacac gttcaataat acgtgctgct ttagtcttct cttcaccatc acgagtcatc
    36781 gctaaatgat gttccaattc actatagtca aaattagaac taaagaatgt tggtaattca
    36841 tgaaccattc gataatgtag caaaggtcca attacctcat ctctcaccca tggagtcact
    36901 tcttcagccc caatatcatc aagcattaaa atgtttgctt ctcttacgcg atgtaatttc
    36961 ttttcaaaag aaccatcttt aaagccacct tttaatgttc taataaattc cggtaaataa
    37021 ataattgtcg aacgtacctt cttagatttg agctgattcg caattgcacc tagaataaaa
    37081 gatttacctg tcccaaatgg accataaagg taaaggcctt tcacttgttc cccattagtt
    37141 attgctgtac aaatatcatc tgctgccata gctacatcaa gacggtctcg atgattcata
    37201 taaatatctt tcaatttggc atttaaagta tctcgttgca tatgatgaga tgtaattagc
    37261 tcagcttcaa agcgttcttc gtcgtacttg attttacatg ggcattgtaa atagcgtatt
    37321 ttaattcggt tattatcaac atataactca ggcacatgcc cctttacgaa atttggacaa
    37381 tcagcaaatt tatgaccgtc ataatgtttt tgttgatctt tatactcttg taacacattt
    37441 aagtcttcat caatcatagc attcgttaat tcagctcgat gcgcttccaa aaattgctta
    37501 acatctgggt cattgattac ttcttttttt atcttttcta ttcttttttc aaagtcctgc
    37561 gacgtgttaa ttatactttt aaattgcttc attattgact gtcctcctcc cattttttag
    37621 ataatttatc tagaaatgct tgtcgatctt gctctaattg ttgatcatct acgctattat
    37681 ctttagccga atcttcttca ctaggtttat ctctattttc taaccattta ggtgtttttt
    37741 cttttgaaat acgattacgc tgcccatagt atgaaccacg cttttggtaa tttccgctag
    37801 aaccctcatt tttaggttga ttaacttttt tagcgtaatt atatgcttct ttagctgtct
    37861 taataccttt tttcttccaa tttgatgcta tttccaaaat atacgcttta ggaagtttca
    37921 tatcttcttt taacatgaca aattgcaaca aaatattaat gacgccaaaa gacatttttt
    37981 cacgttcaat taattcttca accattgtct tttgcgatat agttggttct gattcagacc
    38041 aagaagctaa catatcaatt ggactcgttt gttcaagtaa ctcaaaccat tcatcacttt
    38101 gtggctttgg attcacttct gaagatttgc ccgccgaaga tgatgtagca ggagatttca
    38161 cctgtaattt aggcatttga ttttcgtgtt ccattaagta atacgagcgt gcttgtttac
    38221 gcatttcttc aaaggataac tgttgtccac ttgtaattga atttaaaata acatgcttca
    38281 tgccatctgc tgttaaacca tataaagtcg cgagttgtgt aattaaacgc tttgcatctt
    38341 tggtaacaat gtcttgacta ataaaatgtt tacctaacat ttgtctcaac atttcaaagt
    38401 caaaagattc atttgataaa tcgatacctt ggtacggttc attaatcgga atatcacttg
    38461 tatcgatatc tatttttgta gacggcactt taaaaacatc agtaaattgt cttgttacct
    38521 gtttaaattc actcaaatca atttgttgat actcaaagta tttcttcaac tcatgaaatc
    38581 gacgatgctc gacttcacta tataaaaaga ttgacaacat tggatcatta aaaaataaat
    38641 gtgctgaagg cggttgaatt aattggtaaa caaattgtgt ttcttgttca tcatgtttga
    38701 caaacgcctt taacaatcca atcgcttcaa gtaagtccat ttgttgtcta aactctagta
    38761 aattaatttt aagttcattc ataaaaatat aatgagaaag aatcaatgtt tcattatgac
    38821 tttctttaac gaattgagtc ataaaatgat ataaacccac tgcttgcgtt ccaattagcg
    38881 gtgtatacag tcgattcaat acctctaaat gattcgtatt taaatcaaag tgttgcataa
    38941 ctttgaattg atcctttggt cttaagccga attcgaaggc ttgtcgtccc atttaagcat
    39001 cactccgttt gttttcgctt aaaatccctt gcatcgatgc taacaattga tcaacatctt
    39061 taaattcttt atagactgat gcaaatctaa catatgaaac ttgatcaaca tgcattaaca
    39121 agttcataac gtgttcacct atatctcgtg aagacacttc cgtatgacct tcatctcgta
    39181 attgccattc aaccttgtta gttatgtctt caagttgttg atatctaact ggtcgtttct
    39241 cacaagaacg cacaagtcca ttaagtatct tttctcttga aaactgctct cttgtgccat
    39301 cttttttcac aactataagc tgactaactt cgatatgttc aaatgtagtg aaacgtgttc
    39361 cacaattttc acattctctt cgtcttcgaa tggcatttaa ttcatcggca tgccttgaat
    39421 ctacaacttt agattgtgta gaattacatt tcgggcattt cattacatca ccctctttat
    39481 tttgattatg cctaattata ctataaatct agagatgaaa aaagaatccc tcaatttaat
    39541 tcatttaacc aaataatgaa acaataaaaa acattatatc gttacttatt aagtaatttg
    39601 catgacaata ttattgtatt aaaaataaaa aacctaactc cgaagtcaga gttaggctat
    39661 aaattaattg tattaacttg cacttacagt ttcttttgat gtcaaaagtg ctccaatttg
    39721 ctcagcaaca tctacaactc tatttgaata accccattca ttatcatacc aagcaataac
    39781 ttttacttta ttccctgaca tgaccattgt tgattttgca tcaataatag ctgaatttgg
    39841 attagtatta aaatcaacag acactagtgg ttgatgttcg acttctatga taccttctaa
    39901 acctgcattt tcaaaagctt ggtttacttc ttctgcagtt acttcttttt ctaaatcaac
    39961 aactaaatca acgagcgata cattctttgt tggtacacgt aatgccatgc cgtgtaattt
    40021 accttctaat tctggtaata cttcttttaa agctttcgcc gcaccagtag aagtaggaat
    40081 aatgctttca ttacatgaac gtgcacgtct taaatcttta tgtggattat caatattttt
    40141 ttggtcattt gtaatagcgt gaacagtagt cattaaacca ttaactattc caaactgatt
    40201 atttaaaact tttgcaactg gaccaatgca attagtagta catgaagcat tactaaaaat
    40261 gtcaaatgct tctatatcta attggttatc atttacgcct ttaactacca tttgaacatg
    40321 tccacctttt gaaggaccag ttaacaaaac ttttttggca cctgctttaa tatgtgcgat
    40381 ggctttatca ccatgattaa atttaccagt tgcatctata gcaatatcga tatctaattc
    40441 tttccatggc aagttttcag gattgcgatc agcaaccaat ttaattttat gatctccaac
    40501 ttgcaatcca ttttcaatcg gttcaacttt tagattatat tttccatgtg tcgtatcgta
    40561 attgattaaa tgtgcaattg tttcgggtgg ataactagca tttatcgcta ctacatttaa
    40621 atttttattt tgtaatgcaa tacgtaatac cattcttcca attctaccca taccattaat
    40681 tgcaatattc gttgacatta aaagcacccc ttgatcttta tgtgttatac tttatggaat
    40741 tagtatagca tattatatga aatttgaaag cgttttccgt tgatttattt atctttttta
    40801 taaaaaatag tcattactta catacatcaa cgcgtacgta aagtaatgac ttatttatag
    40861 tgtaatatta atcttcttct ccgtaattca gcttttcaat ataaccttct tcttctaaca
    40921 atctctcaag gttttgtttt aattcaagtt tatcccctaa attatcgata acatgatcgg
    40981 ccattcggct ttttttatca atagaaattt ggctatagac acgtgctttc gcatcttcta
    41041 atgacaaatt attacgttgc attaaacgat ccatttgtat actttcagaa gtgtatacaa
    41101 cccacacttc gtctactgta ttttccaatt cattttcaaa taataatgga atatccatga
    41161 ttacattata tccttgtttt aaatattctt gcttttcttc ttccataata tctcgcacga
    41221 taggatgtat gatagcattt aattctaagc gtttttctgg atgattaaac actagatcac
    41281 ccatataacg acgattcatc tcaccatttt catcaattgc ttcatcacca aagacttctc
    41341 gtacttgagc taaaccttta ctcccctttt taacagcttc cctggctgct ttatcagcat
    41401 ctactacttt aaaaccgaat acggataaga gttctgatac tgttgatttt cctgaggcga
    41461 ttccacctgt tagaccaata actttcggca taatttcact ctttctttat ttttgacata
    41521 ctggacaata atgactattt cttgtcgcga tgatttttgt ttcaatttga cttccacaca
    41581 ctttgcatac cggctgctta tatacattaa gatgcaattg catctcacca gtttttccat
    41641 cagcatgacg ataatctgaa atacttgtac cgccatattt aataccttct tctagtactt
    41701 ctctaacata ataaaaaacc atttcttgtt gttggtgtgt taagtctttt acttttttat
    41761 ctggtaaaac acctgcacga aacaacgctt cacatgcgta aatatttcca caacctgcga
    41821 ttactttatg atccaaaatc acttgtttga ttggtttatt cttattagac tgttgatgaa
    41881 ttcgatttaa ataatacgtc aatgcttcat ttgaaaaagg ttcaggcgct atttctaaaa
    41941 atgaaggata agatgctaca gacgcaacat ttctaatttc tccaaaacga cgtatatctg
    42001 aataaattaa ctttttgtca tttgacaact caaaaataac atgccaatgc ttacgataat
    42061 taggtatcat aatatcttca agttcatcta caatgaaaaa accgcccgcc atacctaaat
    42121 gactaattaa tgtacgttgt tctcgtttat tatctagctg aaaaacgata tatttacttc
    42181 ttcgttctac atttgtaatg gtatagcctt ccgataaagt tttaaaagta tctaattcaa
    42241 ttccttttat aattgtttcc ttgccttgag ctttaccttc gattacttta tccgaaaata
    42301 taacgtgttc aattttttga tttataacgt agggttcaat tcctcttttt acatgttcta
    42361 cttctggtaa ttcgggcata ccattaacct cactttattt tgcatcatac caggttgcac
    42421 cataacttga gtctactttt aatggaacat ctaattgcaa tgcattttcc attatctctt
    42481 ctacaaattc actaaatgaa tctacttctg acttaggtac ttcaaaaatt aattcatcgt
    42541 gtacttgtaa taatagttta gcttgatatg ttgtctcttt cattttttga gcaaatttaa
    42601 ccattgccag tttaatgata tctgcagcac tgccctgtat tggcgtattc atagcagtac
    42661 gttcagcaaa gccgcgtaaa ttaaagttac gactcgtaat atcaggaata tagcgtcgac
    42721 gatgtagcaa tgtttccacg taacctaaag ctttggcatc ttttacaata tcagacatat
    42781 attgttttac acctgggaaa ctagctaaat aatcatcaat gaatgctttt gcttttttac
    42841 gagtaatacc taaactttga cttaaaccat aatcacttat cccataaaca attccaaagt
    42901 taactgcttt tgcttgacga cgcattaaac tatcgacttg atcagcttct acaccaaata
    42961 ctttcatagc agttgctgta tgaatatcat cgccgttgat aaatgcttct ttcatactct
    43021 catcttgtgt aatgtgtgct aatacacgca attcaatttg agaataatct gctgataata
    43081 taacgctatc ttttgaagtt ggtttaaagg cttttcttat tttacgccct tcttcaagtc
    43141 taaccggaat attttgtaaa ttaggatcta cacttgataa acgtccagtt tgcgctaaag
    43201 tttgattaaa acgtgtatgg atacgttgat catcactaat taccttttgc aatccttcaa
    43261 cataagtaga ctgtaactta gataattgac gatattctaa aatataatca atgataggat
    43321 gttcaccttg caattgctct aagacatcta cagcagtaga atatcctgtt ttcgtctttt
    43381 taataacagg taattgtaat gtctcaaaca atacaacacc taattgctta ggagaattta
    43441 tattaaaatc ttcaccagct gcatcatgga tatttcgaat caagacgtct aatttttctt
    43501 gaatttcttt ttccatttct tctaaatcat gaacatctgt aaatatacca atttcttcca
    43561 tttcacttaa aatcttagct agcggtagct ctaaatcagc caagagttct acctgattgt
    43621 attcttctaa ttgtttatcc atatttggtt tcgcaaagta aattgcatca gtaatagaag
    43681 caacatatgg atttaaaaca tcatcttcag gtaccttaaa tttcttacct tttccatata
    43741 tactcacatc gtctttcaca aaactttgac cgtacaatga aacaactgat tgaacatcac
    43801 taatcgtacg agatggatca attatataac tggccaacat aatatcgaaa gaaatatttt
    43861 gaatatcaat ccccaatcta tgtgatgcta catatgtttt tttagcatca tatacgactt
    43921 ttttcgaatt cggattttct aaccatgaaa ctagttcgac ataattattt atgtcatccg
    43981 cattaattac aatatgtttc tcacctgtaa ataaagagaa ttttaaaata ttatttcgca
    44041 aatagttacc accgtctaat tcgaaatgga tggccgcttc tttcaatgaa gtaaaatcaa
    44101 tattatcaaa agacgtttca atttcaaatg tcttttctat tgcatcttca acgcttgctg
    44161 attgatcaat gtcagccaac aattgtttga attctaactt cttaaacaat tcgatttttt
    44221 cttgttgttc atcttgatga gtcattaacg tatcttcaag ttttacttca atcggactat
    44281 ctacattaat cgttgctaat tctttactca ttaatgcatc ttctttgcta ttttgaagtt
    44341 tttcttttaa ctttttacct gaaatttcat ctaaatgttc atagacacct tctactgtgt
    44401 caaattggtt tagcaatttt attgctgttt tctctccaac acctgcaaca cctggtatat
    44461 tatcagaagt atctcccatt aatcctttca tatcaataat ttgattaggt gttaacccgt
    44521 tgtatttttc cgcaataaag tcaggtgtat aatgatcaac atcagtaaca ccttttttag
    44581 tgtaataaat ggttacatta tccgttgcaa gttgtgttaa atctcggtct cccgtaataa
    44641 taattgtctg aaagcccgct ttatctgctt ctttacttaa agttccgata atatcatctg
    44701 cctcatagtt atctaattca taacgtttaa tatgataagc atctaataat tggcgaatat
    44761 aaggaaattg ctcacttagt tcaggcggcg ttttctggcg tccaccttta tattcactat
    44821 atttttcatg tctgaaagtc gttttacctg catcaaacgc tactaaaaaa tgatttggct
    44881 tttcttcttt taaaatcttc tctagtaaca ttgcaaaacc atatactgca ttggtatgaa
    44941 tgcctgcttt gtttgataac aaaggtaatg cataaaaagc tctaaaactt aagctattac
    45001 catcgattaa tactaattta ttcacaattt taacctccag aactaattta
//
LOCUS       DAXWFK010000003        42826 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.003, whole genome shotgun sequence.
ACCESSION   DAXWFK010000003 DAXWFK010000000
VERSION     DAXWFK010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 42826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42826
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.003"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            75..1259
                     /gene="rocD"
                     /locus_tag="ACGEXS_000102"
     CDS             75..1259
                     /gene="rocD"
                     /locus_tag="ACGEXS_000102"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine aminotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HFF8011154.1"
                     /translation="MNSIIELTDYYSSNNYAPLKLVISKGKGVKVWDTDGKQYIDCIS
                     GFSVANQGHCHPTIVKAMTEQASKLSIISRVLYSDNLGKWEEKICHLAKKDKVLPLNS
                     GTEAVEAAIKIARKWGSEVKGITDGQVEIIAMNNNFHGRTLGSLSLSNHDAYKAGFHP
                     LLQGTTTVDFGDIEQLTQAISPNTAAIILEPIQGEGGVNIPPKGYIQAVRQLCDKHQI
                     LLIADEIQVGLGRTGKWFAMEWEQVVPDIYILGKALGGGLYPVSAVLANNDVMRVLTP
                     GTHGSTFGGNPLAIAISTAALDVLKDEQLVERSERLGSFLLKALLQLKHPSIKEIRGR
                     GLFIGIELNTDAAPFVDQLIQRGILCKDTHRTIIRLSPPLVIDKEEIHQIVAAFQDVF
                     KN"
     gene            1298..2329
                     /gene="argC"
                     /locus_tag="ACGEXS_000103"
     CDS             1298..2329
                     /gene="argC"
                     /locus_tag="ACGEXS_000103"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFF8011155.1"
                     /translation="MIKVGIVGGSGYGAIELIRLLQTHPHVTIAHIYSHSKVDEPLKL
                     TFPHLQHIMQHFEALTVDNNDCDVIFFATPAPVSKTCIPPLVEKGIHVIDLSGAFRIK
                     NREIYEAYYKETAAAQDDLNHAIYSISEWQSFDNNGTKLISNPGCFPTATLLALHPLI
                     SEKIVDLSSIIIDAKTGVSGAGRSLSQRVHFSEMNENLSAYAIGNHKHKPEIEQYLSI
                     IAGQDVSVIFTPHLVPMTRGILSTIYVKLSSEYTTESLHKLMTSYYANQPFVRIRDIG
                     TFPTTKEVLGSNYCDIGIYVDETTQTAILVSVIDNLVKGASGQAIQNLNILYDFEVTT
                     GLNQSPVYP"
     gene            2341..3582
                     /gene="argJ"
                     /locus_tag="ACGEXS_000104"
     CDS             2341..3582
                     /gene="argJ"
                     /locus_tag="ACGEXS_000104"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HFF8011156.1"
                     /translation="MKHQETTSQQYNFSIIKHGDISTPQGFTAGGMHIGLRANKKDFG
                     WIYSSSLASAAAVYTLNQFKAAPLIVTEDTLQKSKGKLQALVVNSANANSCTGQQGID
                     DARQTQTWVAQQLQIPSEHVAVASTGVIGEYLPMDKIKTGTEHIKDANFATPGAFNEA
                     ILTTDTCTKHIAVSLKIDGKTVTIGGSTKGSGMIHPNMATMLAFITTDASIESNTLHQ
                     LLKSSTDHTFNMITVDGDTSTNDMVLVMANHQVEHQILSQDHPQWETFVDAFNFVCTF
                     LAKAIARDGEGATKLISVNVSGAKSISDARKIGKTIVSSNLVKSAIFGEDANFGRIIT
                     AIGYSGCEIDPNCTYVQLNQIPVVDKGMAVLFDEQAMSNTLTHENVTIDVQLGLGNAA
                     ATAYGCDLSYDYVRINASYRT"
     gene            3598..4362
                     /gene="argB"
                     /locus_tag="ACGEXS_000105"
     CDS             3598..4362
                     /gene="argB"
                     /locus_tag="ACGEXS_000105"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFF8011157.1"
                     /translation="MKFIVIKIGGSTLSDMHPSIINNIKHLRSNNIYPIIVHGGGPFI
                     NEALSNQQIEPHFVNGLRVTDKATMTITKHTLIADVNTALVAQFNQHQCSAIGLCGLD
                     AQLFEITSFDQQYGYVGVPTALNKDALQYLCTKFVPIINSIGFNNHDGEFYNINADTL
                     AYFIASSLKAPIYVLSNIAGVLINDVVIPQLPLVDIHQYIEHGDIYGGMIPKVLDAKN
                     AIENGCPKVIIASGNKPNIIESIYNNDFVGTTILNS"
     gene            4632..5111
                     /locus_tag="ACGEXS_000106"
     CDS             4632..5111
                     /locus_tag="ACGEXS_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFF8011158.1"
                     /translation="MGIFIYAGIIGGLLSGIVKLGWEVMFPPRTPERNATNPPQELLQ
                     QLGFSSEFTHQTYTFSNMELPWVSFIVHFSFSIVIAIIYCILVKKYAYLAMGQGAVFG
                     IAIWVLFHLIIMPIMHTVPAVWDQPFQEHLSEFFGHIVWMMTIELVRQHFVYRYKLN"
     gene            complement(5439..6083)
                     /locus_tag="ACGEXS_000107"
     CDS             complement(5439..6083)
                     /locus_tag="ACGEXS_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFF8011159.1"
                     /translation="MTVFVMQLQSNLKSIEELISQSRWSYKKPRTVNYRYNQDKLMHR
                     LGDILVQYGIQHDTGLLPHEWHYHISPRGKADIVQHNRDGQPIYVSLSYSYPYIVCVV
                     DKEPVGIDIEKISQRLDWRTLVTCFSTNEAHQICSLNDFYQIWTQKESFTKLIGEGLI
                     KGLDIYDMTQSHFYQSREVKFKQFIFDQFMVQVCFLGQAPWGYKKVSVFQLLSS"
     gene            complement(6096..13271)
                     /locus_tag="ACGEXS_000108"
     CDS             complement(6096..13271)
                     /locus_tag="ACGEXS_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HFF8011160.1"
                     /translation="MIMGNLRFQQEYFRIYKNNTESTTHRNAYWVKLAKNVEATKMMY
                     ALSTIVQQHASIRHFFDVTTDDNLTMILHEFLPFIEIKQVPSSSANYDLEAFFKQELS
                     TYHFNDSPLFKVKLFQFADAAYILLDFHVSIFDDSQIDIFLDDLCNAYRGNTVINNTR
                     QHAHINRNDDKDNQDASHIALDSNYFRLENNSDIHIDSYFPIKHPFEQALYQTYLIDD
                     MTSIDMASLAVSVYLANHIMSQQHDVTLGIHVPSHLPNDLHGNIVPLTLTIDAKDVCQ
                     RFTTDFNKCVLQNMSQLQCAKSSLSLETIFHCYHHMMSCCNDVIEDVHQIHDAHTSLA
                     DIEIFPHQHGFKIIYNSAAYDLLSIETLSDLVRNIYLQITEENGNKRTTVDELNLMTE
                     RDIQLYDDINLSLPEIDDAQTVVTLFEQQVEATPNHVAVQFDGVFITYQTLNARANDL
                     AHRLRNQYGVEPNDRVAVIAEKSIEMIIAMIGVLKAGGAYVPIDPNYPSDRQEYILKD
                     VTPKVVITYQALYENGKQNINHIDLNKIAWKNIDNLSKCNTLEDHAYVIYTSGTTGNP
                     KGTLIPHRGIVRLVHQNHYVPLNEETTILLSGTIAFDAATFEIYGALLNGGKLIVAKK
                     EQLLNPIAVEQLINENDVNTMWLTSSLFNQIASERIEVLVPLKYLLIGGEVLNAKWVD
                     LLNQKPKHPQIINGYGPTENTTFTTTYNIPNKVPNRIPIGKPILGTHVYIMQGERRCG
                     VGIPGELCTSGFGLAAGYLNQPELTADKFIKDSNINQLMYRSGDIVRLLPDGNIDYLY
                     RKDKQVKIRGFRIKLSEVEHALERIQGINKAVVIVQNHDQDQYIVAYYEAMHTLSHNK
                     IKSQLRMTLPEYMIPVNFMHIEQIPITINGKLDKKALPIMDYVDTDAYVAPSTDTEHL
                     LCQIFADILHVNQVGIHDNFFELGGHSLKATLVVNRIEASTGKRLQIGDLLQKPTVFE
                     LAQAIAKVQEQNYEVIPETIVKDDYVLSSAQKRMYLLWKSNHKDTVYNVPFLWRLSSE
                     LNVAQLRQAVQRLIARHEILRTQYIVVDDEVRQRIVADVAVDFEEVNTHFTDEQEIMR
                     QFVAPFNLEKPSQIRVRYIRSPLHAYLFIDTHHIINDGMSNIQLMNDLNALYQHKLLL
                     PLKLQYKDYSEWMSHRDMTKHRQYWLSQFKDEVPILSLPTDYVRPNIKTTNGAMMSFT
                     MNQQMRQLLQKYVEKHQITDFMFFMSVVMTLLSRYARKDDVVVGSVMSARMHKGTEQM
                     LGMFANTLVYRGQPSPDKMWTQFLQEVKEMSLEAYEHQEYPFECLVNDLDQSHDASRN
                     PLFDVMLVLQNNETNHAHFGHSKLTHIQPKSVTAKFDLSFIIEEDRDDYTINIEYNTD
                     LYHSETVRHMGNQCMIMIDYILKHQDTLQICDIPNGTEELLNWVNTHVNDRMLNVPGN
                     KSIISYFNEVVSRQGNHVALVMNDLTMTYETLRNYVDAIAHMLLSNGVGNGQRVALFT
                     ERSFEMIAAMLATVKVGASYIPIDIDFPNKRQGAILEDAKVTAVMSYGVEIETTLPVI
                     QLENAKGFVESKENEQYDDLHGNQLENTAMLDNEMYAIYTSGTTGMPKGVAIRQRNLL
                     NLVHAWSTELQLGDNEVFLQHANIVFDASVMEIYCCLLNGHTLVIPDREERVNPEQLQ
                     QLINKHRVTVASIPLQMCSVMEDFYIEKLITGGATSTASFVKYIEKHCGTYFNAYGPS
                     ESTVITSYWSHHCGDLIPETIPIGKPLSNIQVYIMSDGLLCGIGMPGELCIAGDSLAI
                     GYINRPELMADKWQNNPFGKGKLYHSGDLARYTSDGQIEFLGRIDKQVKVNGYRIELD
                     EIENAILAIRGISDCVVTVSHFDTHDILNAYYVGEQQVEQDLKQYLNDQLPKYMIPKT
                     ITHIDCMPLTTNDKVDTTRLPNPSPIQQSNKVYSEPSNEIEQTFVDVFGEVLKQNDVG
                     VDDDFFELGGNSLEAMLVVSHLKRFGHHISMQTLYQYKTVRQIVNYMYQNQQSLVALP
                     DNLSELQKIVMSRYNLGILEDSLSHRPLGNTLLTGATGFLGAYLIEVLQGYSHRIYCF
                     IRADNEEIAWYKLMTNLNDYFSEETVEIMLSNIEVIVGDFECMDDVVLPENMDTIIHA
                     GARTDHFGDDDEFEKVNVQGTVDVIRLAQQHHARLIYVSTISVGTYFDIDTEDVTFSE
                     ADVYKGQLLTSPYTRSKFYSELKVLEAVNNGLDGRIVRVGNLTNPYNGRWHMRNIKTN
                     RFSMVMNDLLQLDCIGVSMAEMPVDFSFVDTTARQIVALAQVNTPQIIYHVLSPNKMP
                     VKSLLECVKRKEIELVSDESFNEILQKQDMYETIGLTSVDREQQLAMIDTTLTLKIMN
                     HISEKWPTITNNWLYHWAQYIKTIFNK"
     gene            complement(13718..14968)
                     /locus_tag="ACGEXS_000109"
     CDS             complement(13718..14968)
                     /locus_tag="ACGEXS_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFF8011161.1"
                     /translation="MKRLSTTLKVRLISNFLQLIITTAFIPFIALYLTDMLSQSIVGI
                     YLVGLVVLKFPLSIISGYLIEIFPKKLLVLIYQATMVIMLVFMGVFGSHQLWQIIGFC
                     VAYAIFTIVWGLQFPVMDTLIMDAITEDVEHYIYKISYWMTNLSVAIGALLGGLMYGY
                     SMLLLFLIAACIFLIVLFILYIWLPQDRNQVKQSDDKRHASRYQKLQIMNIFRSYKLV
                     LKDRNYMLLISGFSIIMMGEFSISSYIAIRLKDQFETISIGSYDITGAKMLAILLMIN
                     TVVVILLTYSISKVVLKIDFKKALITGLLIYIVGYSGLTYLNQFGLLVVFMIIATVGE
                     IIYSPIVSEQRFKIIPKAKRGTYSAVNALGIHFSETLARLWIVLGVFLTSLQMGLYMF
                     IVLTIGASMLVAGVFGGQKQVNTN"
     gene            complement(15354..16379)
                     /locus_tag="ACGEXS_000110"
     CDS             complement(15354..16379)
                     /locus_tag="ACGEXS_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent formate dehydrogenase"
                     /protein_id="HFF8011162.1"
                     /translation="MKIVALFPEAVEGQENQLLNTKKALGLKTFLEERGHEFIILADN
                     GEDLDKHLPDMDVIISAPFYPAYMTRERIEKAPNLKLAITAGVGSDHVDLAAASEHNI
                     GVVEVTGSNTVSVAEHAVMDLLILLRNYEEGHRQSVEGEWNLSQVGNHAHELQHKTIG
                     IFGFGRIGQLVAERLAPFNVTLQHYDPINQQDHKLSKFVSFDELVSTSDAITIHAPLT
                     PETDNLFDKDVLSRMKKHSYLVNTARGKIVNRDALVEALASEHLQGYAGDVWYPQPAP
                     ADHPWRTMPRNAMTVHYSGMTLEAQKRIEDGVKDILERFFNHEPFQDKDIIVASGRIA
                     SKSYTAK"
     gene            complement(16552..16911)
                     /locus_tag="ACGEXS_000111"
     CDS             complement(16552..16911)
                     /locus_tag="ACGEXS_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HFF8011163.1"
                     /translation="MNTIDTHTKEQQFSNLVRSYRKEYVGKGPNSIRVSFKDNWAIAH
                     MTGVLSKVESFYLNDKRNESMLHYTRTEKIKQMYKEIDVNEMESLVGAKFVKLFTDID
                     LNDDEVISIFVFDKSIE"
     gene            complement(17128..18159)
                     /locus_tag="ACGEXS_000112"
     CDS             complement(17128..18159)
                     /locus_tag="ACGEXS_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HFF8011164.1"
                     /translation="MTLETLIKEQLDPHLVEVDEGTYYPRTFIQQLFVDGYFGEAALR
                     KNAEVIEAVSQSCLTTGFCLWCQLAFSTYLENATQPHLNNDLQQQLLSGEILGATGLS
                     NPMKSFNDLEKLNLEHTYVDGQLVVSGRMPAVSNIQEDHYFGAISKHESSDEFVMFIL
                     RANQDGITLVEKTNFLGVNGSATYQITLNQVVVPQSQIITHDAKQFAATIRPQFIAYQ
                     IPIGLGSIKSSLELIDAFSNVQNGINQYLEYDVEAFKKRYRQLREEYYAILDDGNLTS
                     HLNELISLKKDIGYLLLDVNQASVVNGGSRAYTPYSPQVRKLKEGFFFAALTPTLRHL
                     GKLEAELKG"
     gene            complement(18172..18933)
                     /locus_tag="ACGEXS_000113"
     CDS             complement(18172..18933)
                     /locus_tag="ACGEXS_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8011165.1"
                     /translation="MTRPTNNKFILPIITFIIFLGIWEMVIIIGHYQPVLLPGPALVG
                     KSIWSFIVTGEIFQHLAISLWRFVAGFVVALLVAIPLGFLLGRNRWLYNAIEPLFQLI
                     RPISPIAWAPFVVLWFGIGSLPAIAIIFIAAFFPIVFNTIKGVRDIEPQYLKIAANLN
                     LTGWSLYRNILFPGAFKQIMAGIHMAVGTSWIFLVSGEMIGAQSGLGFLIVDARNMLN
                     LEDVLAAIFFIGLFGFIIDRFISYIEQFILRRFGE"
     gene            complement(18930..19904)
                     /locus_tag="ACGEXS_000114"
     CDS             complement(18930..19904)
                     /locus_tag="ACGEXS_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8011166.1"
                     /translation="MKRLSIIVIIGIFIITGCDWQRTSKERSKNAQNQQVIKIGYLPI
                     THSANLMMTKKLLSQYNHPKYKLELVKFNNWPDLMDALNSGRIDGASTLIELAMKSKQ
                     KGSNIKAVALGHHEGNVIMGQKGMHLNEFNNNGDDYHFGIPHRYSTHYLLLEELRKQL
                     KIKPGHFSYHEMSPAEMPAALSEHRITGYSVAEPFGALGEKLGKGKTLKHGDDVIPDA
                     YCCVLVLRGELLDQHKDVAQAFVQDYKKSGFKMNDRKQSVDIMTHHFKQSRDVLTQSA
                     AWTSYGDLTIKPSGYQEITTLVKQHHLFNPPAYDDFVEPSLYKEASRS"
     gene            complement(19918..20658)
                     /locus_tag="ACGEXS_000115"
     CDS             complement(19918..20658)
                     /locus_tag="ACGEXS_000115"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8011167.1"
                     /translation="MIKIQQLQHHFGSHKVIHNFNLDISKGEIVTFIGKSGCGKSTLL
                     NIIVGFIHPSSGRVIIDNEIKQQPSPDCLMLFQHHNLLPWKTINDNIRIGLQQKISDE
                     EINAQLKLVDLEDRGKHFPEQLSGGMKQRVALCRAHVHKPNVILMDEPLGALDAFTRY
                     KLQDQLVQLKHKTQSTIILVTHDIDEAIYLSDRIVLLGEGCNIISQYEITASHPRSRN
                     DSHLLKIRNEIMETFALNHHQVEPEYYL"
     gene            complement(21000..21512)
                     /locus_tag="ACGEXS_000116"
     CDS             complement(21000..21512)
                     /locus_tag="ACGEXS_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HFF8011168.1"
                     /translation="MTLFLLEANNLDFASTKEELEAKAASLSTKTIPTLIEVQATENL
                     THGYFIVEANDEAEAKQFLTEADISIQLVKEVRLVGKDLDEVKNGDAHVDYLVTWNIP
                     EGITMDQYLARKKKNSVHYEEVPEVEFKRTYVCEDMSKCICLYNAPDEEAVRRARKAV
                     DTPIDGIEKL"
     gene            21691..21894
                     /locus_tag="ACGEXS_000117"
     CDS             21691..21894
                     /locus_tag="ACGEXS_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011169.1"
                     /translation="MDKESVVASLARNKKIAVETMAGQRYIIERILHTNDEKHIHILK
                     PKDVVLDVDSIKEIDENHLNDAT"
     gene            complement(21956..22915)
                     /locus_tag="ACGEXS_000118"
     CDS             complement(21956..22915)
                     /locus_tag="ACGEXS_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFF8011170.1"
                     /translation="MNGKKANTINRYKYFHHVNHQKIQQSSKKTLWASLIITLLFTVI
                     EFVGGLVSNSLALLSDSFHMLSDVLALGLSMLAIYFASKKPTARYTFGYLRFEILAAF
                     LNGLALIVISIWILYEAIVRIIYPQPIESGIMFMIASIGLLVNIILTVILVRSLKQED
                     NINIQSALWHFMGDLLNSIGVIVAVVLIYFTGWRIIDPIISIVISLIILRGGYKITRN
                     AWLILMESVPQHLDTDQIMADIKNIDGILDVHEFHLWSITTEHYSLSAHVVLDKKYEG
                     DDYQAIDQVSSLLKEKYGIAHSTLQIENLQLNPLDEPYFDKLT"
     gene            complement(23576..25063)
                     /locus_tag="ACGEXS_000119"
     CDS             complement(23576..25063)
                     /locus_tag="ACGEXS_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFF8011171.1"
                     /translation="MAVNVRDYIAENYGLFINGEFVKGSSDETIEVTNPATGETLSHI
                     TRAKDKDVDHAVKVAQEAFESWSLTSKSERAQMLRDIGDKLMAQKDKIAMIETLNNGK
                     PIRETTAIDIPFAARHFHYFASVIETEEGTVNDIDKDTMSIVRHEPIGVVGAVVAWNF
                     PMLLAAWKIAPAIAAGNTIVIQPSSSTPLSLLEVAKIFQEVLPKGVVNILTGKGSESG
                     NAIFNHDGVDKLSFTGSTDVGYQVAEAAAKHLVPATLELGGKSANIILDDANLDLAVE
                     GIQLGILFNQGEVCSAGSRLLVHEKIYDQLVPRLQEAFSNIKVGNPQDEATQMGSQTG
                     KDQLDKIQSYIDAAKESDAQILAGGHRLTENGLDKGFFFEPTLIAVPDNHHKLAQEEI
                     FGPVLTVIKVKDDQEAIDIANDSEYGLAGGVFSQNITRALNIAKAVRTGRIWINTYNQ
                     VPEGAPFGGYKKSGIGRETYKGALSNYQQVKNIYIDTSNALKGLY"
     gene            25490..25873
                     /locus_tag="ACGEXS_000120"
     CDS             25490..25873
                     /locus_tag="ACGEXS_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HFF8011172.1"
                     /translation="MRLILIVIGLIFTALGIAGAVLPLLPTTPFLLVAVFCFARSSDR
                     FYNWLINQKIYKEYVENFYLHRGYTLQQKIKILISLYIVIGFSIYMVDVLAVRVGLII
                     MVIIQTAVLFTFVKTLPKSNHKIEE"
     gene            25880..26206
                     /gene="isdI"
                     /locus_tag="ACGEXS_000121"
     CDS             25880..26206
                     /gene="isdI"
                     /locus_tag="ACGEXS_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylobilin-forming heme oxygenase IsdI"
                     /protein_id="HFF8011173.1"
                     /translation="MFMAENRLQLQKGSAEETIERFYNRQGIETIEGFQQMFVTKTLN
                     TEDTDEVKILTVWESEDSFNNWLNSDVFKEAHKNVRLKSDDDGQQSPILSNKVFKYDI
                     GYHYQK"
     gene            complement(26271..27416)
                     /gene="cap8P"
                     /locus_tag="ACGEXS_000122"
     CDS             complement(26271..27416)
                     /gene="cap8P"
                     /locus_tag="ACGEXS_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8P"
                     /protein_id="HFF8011174.1"
                     /translation="MKKIMVIFGTRPEAIKMAPLVKEIDHNGNFEANIVITAQHRDML
                     DSVLSIFDIQADHDLNIMQDQQTLAGLTANALAKLDSIINEEQPDMILVHGDTTTTFV
                     GSLAAFYHQIPVGHVEAGLRTHQKYSPFPEELNRVMVSNIAELNFAPTVIAAKNLLFE
                     NKDKERIFITGNTVIDALSTTVQNDFVSTIINKHKGKKVVLLTAHRRENIGEPMHQIF
                     KAVRDLADEYKDVVFIYPMHRNPKVRAIAEKYLSGRNRIELIEPLDAIEFHNFTNQSY
                     LVLTDSGGIQEEAPTFGKPVLVLRNHTERPEGVEAGTSRVIGTDYDNIVRNVKQLIED
                     DEAYQRMSQANNPYGDGQASRRICEAIEYYFGLRTDKPDEFVPLRHK"
     gene            complement(27493..28755)
                     /gene="cap8O"
                     /locus_tag="ACGEXS_000123"
     CDS             complement(27493..28755)
                     /gene="cap8O"
                     /locus_tag="ACGEXS_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8O"
                     /protein_id="HFF8011175.1"
                     /translation="MKLTVVGLGYIGLPTSIMFAKHGVDVLGVDINQQTIDKLQSGQI
                     SIEEPGLQEVYEEVLSSGKLKVSTTPDASDVFIIAVPTPNNDDQYRSCDISLVMRALD
                     SILSFLEKGNTIIVESTIAPKTMDDFVKPVIENLGFTIGEDIYLVHCPERVLPGKILE
                     ELVHNNRIIGGVTEACIEAGKRVYRTFVQGEMIETDARTAEMSKLMENTYRDVNIALA
                     NELTKICNNLNINVLDVIEMANKHPRVNIHQPGPGVGGHCLAVDPYFIIAKDPENAKL
                     IQTGREINNSMPAYVVDTTKQIIKVLSGNKVTVFGLTYKGDVDDIRESPAFDIYELLN
                     QEPDIEVCAYDPHVELDFVEHDMSHAVKDASLVLILSDHSEFKNLSDSHFDKMKHKVI
                     FDTKNVVKSSFEDVSYYNYGNIFNFIDK"
     gene            complement(28809..29696)
                     /gene="capN"
                     /locus_tag="ACGEXS_000124"
     CDS             complement(28809..29696)
                     /gene="capN"
                     /locus_tag="ACGEXS_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide type 5/8 biosynthesis
                     epimerase CapN"
                     /protein_id="HFF8011176.1"
                     /translation="MRKNILITGVHGYIGNALKDKLIEQGHQVDQINVRNQLWKSTSF
                     KDYDVLIHTAALVHNNSPQARLSDYMQVNMLLTKQLAQKAKAEDVKQFIFMSTMAVYG
                     KEGHVGKSDQVDTQTPMNPTTNYGISKKFAEQALQELISDSFKVAIVRPPMIYGAHCP
                     GNFQRLMQLSKRLPIIPNINNQRSALYIKHLTAFIDQLISLEVTGVYHPQDSFYFDTS
                     SVMYEIRRQSHRKTVLINMPSMLNKYFNKLSVFRKLFGNLIYSNTLYENNNALEIIPG
                     KMSLVIADIMDETTTKDKA"
     gene            complement(29696..30253)
                     /gene="cap8M"
                     /locus_tag="ACGEXS_000125"
     CDS             complement(29696..30253)
                     /gene="cap8M"
                     /locus_tag="ACGEXS_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8M"
                     /protein_id="HFF8011177.1"
                     /translation="MKRLFDVVSSIYGLVVLSPILLITALLIKMESPGPAIFKQKRPT
                     INNELFNIYKFRSMKIDTPNVATDLMDSTSYITKTGKVIRKTSIDELPQLLNVLKGEM
                     SIVGPRPALYNQYELIEKRTKANVHTIRPGVTGLAQVMGRDDITDDQKVAYDHYYLTH
                     QSMMLDMYIIYKTIKNIVTSEGVHH"
     gene            complement(30264..31469)
                     /gene="cap8L"
                     /locus_tag="ACGEXS_000126"
     CDS             complement(30264..31469)
                     /gene="cap8L"
                     /locus_tag="ACGEXS_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8L"
                     /protein_id="HFF8011178.1"
                     /translation="MSEKKILILCQYFYPEYVSSATLPTQLAEDLIANHINVDVMCGW
                     PYEYSNHKQVSKTEMHRGIRIRRLKYSRFNNKSKVGRIINFFSLFSKFVINIPKMLKY
                     DQILVYSNPPILPLIPDVLHRLLKKKYSFVVYDIAPDNAIKTGATRPGSMIDKLMRYI
                     NRHVYKNAENVIVLGTEMKNYLLNHQISKNADNIHVIPNWYDMRQLQDNRIYNDTFKA
                     YREQYDKILLYSGNMGQLQDMETLISFLKLNKDQSQTLTILCGHGKKFADVKTAIEDH
                     RIENVKMFEFLTGTDYADVLKIADVCIASLIKEGVGLGVPSKNYGYLAAKKALVLIMD
                     KQSDIVQHVEQYDAGIQIDNGDAHAIYNFINTHSSKELHEMGERAHQLFKDKYTREIN
                     TMKYYNLLK"
     gene            complement(31470..32675)
                     /locus_tag="ACGEXS_000127"
     CDS             complement(31470..32675)
                     /locus_tag="ACGEXS_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HFF8011179.1"
                     /translation="MAKKVFIMDSVKTIIGTLLIALGLQFLAYPIINQRVGNEAFGSI
                     LTIYTIITITSVVLGNTLNNIRLINMNLYKSNHYYWKFASILLISILIESIALIIVFL
                     YFFNLNIIDIIFLILLNILMCLRIYLNVFFRMTLKYNQILYIALIQFLGLLIGLFLYY
                     LTQNWIVCFITSELFATIYTLVKLRGLTIGEYQSEDNNVVKDYVMLLSTNSLNNLNLY
                     LDRLILLPIIGGTAVTISFLSTFIGKMLATFLYPINNVVLSYISVNESDNIKKQYLKT
                     NLIAIAALCLVMIICYPITIIIVSLLYNIDSSLYSKFIILGNIGVLFNAVSIMIQTLN
                     TKHASITLQANYMTLHTITFIFITILMTIAFGLNGFFWTTLFSNIIKYVILNIIGLKS
                     KFINKKDVD"
     gene            complement(32668..33834)
                     /locus_tag="ACGEXS_000128"
     CDS             complement(32668..33834)
                     /locus_tag="ACGEXS_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFF8011180.1"
                     /translation="MKFFVLCAIISMNIFIVISTFTKEVLGFPIEPVYYSTMVGIALI
                     TTVFAIYKIIVTQEIPRGLILLIAICLLYLAFYYFSPDKEEKLAKNNILFFLTWAVPA
                     AISGIYIKYINKATVERFFKLVFFIFSVSFIFVILIPKLTGEIPSYINFGLMNYQNAS
                     YLSAFTAGLGIYFIMKGSVKHKWIYVLFTIIDIPIVFIPGGRGGAILLILYGLFAFIL
                     ITFKRGIPIAVKSIMYIFALSISSVLIYFLFTKGSNTRTFSYLQGGTLNLEGTSGRGP
                     IYEKGIYFIQQSSLLGYGPFNYYKLIGNIPHNIIIELILSFGLLGFFIIMICILLLVY
                     KMIRNYDPNTIDLLVMFIAIYPITLLMFSSNYLVVSEFWFVLFYFITKGRRHHG"
     gene            complement(33848..34957)
                     /locus_tag="ACGEXS_000129"
     CDS             complement(33848..34957)
                     /locus_tag="ACGEXS_000129"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFF8011181.1"
                     /translation="MRILNIVSSNIVQDPRVLKQIETIKGVTDDYKIVGMNNSQATNK
                     RLENLDCNYRLLGSKVDPKNILSKLIKRIRFATGVIREIKAYKPDVIHANDFDVLLMV
                     YLSNYKKANIVYDAHEIYAKNAFINKVPLISKFVESIEKHIVKHRVNAFVTVSHAAKE
                     YYQSKGYKKEANVITNAPILNDSREFKEIENFKEIVYQGQIVMDRGYEEFIIASSAFK
                     QNAPSFIIRGFGPHEEVIKELISYNPENIRLDKPVEVKELVDKLAESNVGVVLTKPVS
                     INFEYTVSNKIFECIHAGLPVILSPVKEHIYLNEKYKFGIVLKEVTPLEIEKAVRKLR
                     DNHDLFNHLRQNAIKASKILNWQIESERLVELYKF"
     gene            complement(34962..35588)
                     /locus_tag="ACGEXS_000130"
     CDS             complement(34962..35588)
                     /locus_tag="ACGEXS_000130"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HFF8011182.1"
                     /translation="MRIAIEKIIGLLKNQSSKESNVKIHRLAYITNSKFDGNNYIDRW
                     CKIRNSHIGEYSYIGFGSDFNNVEVGRYCSISSDVKIGLGKHPTHFFSSSPIFYSNNN
                     PFNIKQKFIDFNDQPSRTTIKNDVWIGANVIIMDGLTINTGAVIAAGSVVTKNVGAYE
                     VVGGVPAKVIKKRFDNKTIEKLLESKWWEKTPDKLKGFSVEYLNKKDT"
     gene            complement(35591..36715)
                     /gene="cap8G"
                     /locus_tag="ACGEXS_000131"
     CDS             complement(35591..36715)
                     /gene="cap8G"
                     /locus_tag="ACGEXS_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8G"
                     /protein_id="HFF8011183.1"
                     /translation="MEKLKLMTIVGTRPEIIRLSSTIKACDQYFNQILVHTGQNYDYT
                     LNQIFFDDLELRQPDHYLEAVGSNLGETMGNIIAKTYDVLLREQPDALLILGDTNSCL
                     AAVSAKRLKIPVFHMEAGNRCFDQNVPEEINRKIVDHVSDVNLPYTEHSRRYLLDEGF
                     NKANIFVTGSPMTEVIEAHRDKINHSDVLNKLGLEPQQYILVSAHREENIDNEKNFKS
                     LMNAINDIAKKYKMPVIYSTHPRSWKKIEESKFEFDPLVKQLKPFGFFDYNALQKDAF
                     VVLSDSGTLSEESSILKFPGVLIRTSTERPEVLDKGTVIVGGITYNNLIQSVELAREM
                     QNNNEPMIDAIDYKDTNVSTKVVKIIQSYKDIINRNTWRK"
     gene            complement(36719..37828)
                     /gene="cap8F"
                     /locus_tag="ACGEXS_000132"
     CDS             complement(36719..37828)
                     /gene="cap8F"
                     /locus_tag="ACGEXS_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8F"
                     /protein_id="HFF8011184.1"
                     /translation="MNIVITGAKGFVGKNLKADLTSTTDHHIFEVHRQTKEEELESAL
                     LKADFVVHLAGVNRPEHDKEFSLGNVSYLDHVLDILTRNTKKPAILLSSSIQATQDNP
                     YGESKLQGEQLLREYAEEYGNTVYIYRWPNLFGKWCKPNYNSVIATFCYKIARNEEIQ
                     VNDRNVELTLNYVDDIVAEIKRAIEGTPTIENGVPTVPNVFKVTLGEIVDLLYKFKQS
                     RLDRTLPKLDNLFEKDLYSTYLSYLPSTDFSYPLLMNVDDRGSFTEFIKTPDRGQVSV
                     NISKPGITKGNHWHHTKNEKFLVVSGKGVIRFRHVNDDEIIEYYVSGDKLEVVDIPVG
                     YTHNIENLGDTDMVTIMWVNEMFDPNQPDTYFLEV"
     gene            complement(37841..38869)
                     /gene="cap8E"
                     /locus_tag="ACGEXS_000133"
     CDS             complement(37841..38869)
                     /gene="cap8E"
                     /locus_tag="ACGEXS_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8E"
                     /protein_id="HFF8011185.1"
                     /translation="MFDDKILLITGGTGSFGNAVMKQFLDSNIKEIRIFSRDEKKQDD
                     IRKKYNNSKLKFYIGDVRDSQSVETAMRDVDYVFHAAALKQVPSCEFFPVEAVKTNII
                     GTENVLQSAIHQNVKKVICLSTDKAAYPINAMGISKAMMEKVFVAKSRNIRSEQTLIC
                     GTRYGNVMASRGSVIPLFIDKIKAGEPLTITDPDMTRFLMSLEDAVELVVHAFKHAET
                     GDIMVQKAPSSTVGDLATALLELFEADNAIEIIGTRHGEKKAETLLTREEYAQCEDMG
                     DYFRVPADSRDLNYSNYVETGNEKITQSYEYNSDNTHILTVEEIKEKLLTLEYVRNEL
                     NDYKASMR"
     gene            complement(38859..40682)
                     /gene="cap8D"
                     /locus_tag="ACGEXS_000134"
     CDS             complement(38859..40682)
                     /gene="cap8D"
                     /locus_tag="ACGEXS_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8D"
                     /protein_id="HFF8011186.1"
                     /translation="MAHLSVKLRLLILALIDSLIVTFSVFVSYYILEPYFKTYSVKLL
                     ILAAISLFISHHISAFIFNMYHRAWEYASVSELILIVKAVTTSIVITMVVVTIVTGNR
                     PFFRLYLITWMMHLILIGGSRLFWRIYRKYLGGKSFNKKPTLVVGAGQAGSMLIRQML
                     KSDEMKLEPVLAVDDDEHKRNITITEGVKVQGKIADIPELVRKYKIKKIIIAIPTIGQ
                     ERLKEINNICHMDGVELLKMLNIEDVMSGELEVNQLKKVEVEDLLGRDPVELDMDMIS
                     NELTNKTILVTGAGGSIGSEICRQVCNFYPERIILLGHGENSIYLINRELRNRFGKNV
                     DIVPIIADVQNRARMFEIMETYKPYAVYHAAAHKHVPLMEDNPEEAVRNNILGTKNTA
                     EAAKNAEVKKFVMISTDKAVNPPNVMGASKRIAEMIIQSLNDETHRTNFVAVRFGNVL
                     GSRGSVIPLFKSQIEEGGPVTVTHPEMTRYFMTIPEASRLVLQAGALAEGGEVFVLDM
                     GEPVKIVDLARNLIKLSGKKEDDIRITYTGIRPGEKMFEELMNKDEVHPEQVFEKIYR
                     GKVQHMKCNEVEVIIQDIVNDFSKEKIINYANGKKGDNYVR"
     gene            complement(40702..41466)
                     /gene="cap8C"
                     /locus_tag="ACGEXS_000135"
     CDS             complement(40702..41466)
                     /gene="cap8C"
                     /locus_tag="ACGEXS_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8C"
                     /protein_id="HFF8011187.1"
                     /translation="MIDIHNHILPNIDDGPTNETEMMDLLKQATTQGVTEIIVTSHHL
                     HPRYTTPIEKVKSCLNHIESLEEVQALNLKFYYGQEIRITDQILNDIDRKVINGINDS
                     RYLLIEFPSNEVPHYTDQLFFELQSKGFVPIIAHPERNKAISQNLDILYDLINKGALS
                     QVTTASLAGISGKKIRKLAIQMIENNLTHFIGSDAHNTEIRPFLMKDLFNDKKLRDYY
                     EDMNGFISNAKLVVDDKKIPKRMPQQDYKQKRWFGL"
     gene            complement(41469..42155)
                     /gene="cap8B"
                     /locus_tag="ACGEXS_000136"
     CDS             complement(41469..42155)
                     /gene="cap8B"
                     /locus_tag="ACGEXS_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8B"
                     /protein_id="HFF8011188.1"
                     /translation="MSKKENTTTTLFVYEKPKSTISEKFRGIRSNIMFSKANGEVKRL
                     LVTSEKPGAGKSTVVSNVAITYAQAGYKTLVIDGDMRKPTQNYIFNEQNNNGLSSLII
                     GRTTMSEAITSTEIENLDLLTAGPVPPNPSELIGSERFKELVDLFNKRYDIIIVDTPP
                     VNTVTDAQLYARAIKDSLLVIDSEKNDKNEVKKAKALMEKAGSNILGVILNKTKVDKS
                     SSYYHYYGDE"
     gene            complement(42171..>42826)
                     /gene="capA"
                     /locus_tag="ACGEXS_000137"
     CDS             complement(42171..>42826)
                     /gene="capA"
                     /locus_tag="ACGEXS_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="capsular polysaccharide type 5/8 biosynthesis
                     protein CapA"
                     /protein_id="HFF8011189.1"
                     /translation="ELTKIKEVLQKNLKILIILPLLFLIISAIVTFFVLSPKYQANTQ
                     ILVNQTKGDNPQFMAQEVQSNIQLVNTYKEIVKSPRILDEVSKDLNDKYSPSKLSSML
                     TITNQENTQLINIQVKSGHKQDSEKIANSFAKVTSKQIPKIMSVDNVSILSKADGTAV
                     KVAPKTVVNLIGAFFLGLVVALIYIFFKVIFDKRIKDEEDVEKELGLPVLGSIQKFN"
BASE COUNT        13741 a         8111 c         6306 g        14668 t
ORIGIN      
        1 gacacttata ttgaacaact gtaatatatt aatattaatt ctttaaaatg tataaatata
       61 aaggagggag accgatgaat tcaatcattg aattaactga ttattatagc tctaataatt
      121 atgcaccact taagcttgtc atttctaaag gtaaaggtgt caaagtttgg gatactgatg
      181 gcaaacaata tatagattgc atttcgggtt tttcagttgc aaaccaaggc cattgtcatc
      241 caacaattgt taaagcgatg acagaacaag cttcaaagtt gtctatcatt tcacgtgtcc
      301 tttatagtga caatctcggg aaatgggaag aaaaaatttg tcatcttgct aagaaagaca
      361 aagtactccc ccttaactct ggtactgaag ctgttgaagc agccattaaa attgctagaa
      421 aatggggctc tgaagttaaa ggcattactg acggacaagt tgaaatcatc gctatgaata
      481 acaattttca cggtcgtaca cttggctcat tatcactatc taaccacgac gcatataaag
      541 caggatttca ccccctactt caaggcacta caacagtaga ttttggagac attgaacaat
      601 taacacaagc tatttcaccg aatacagcag caattatttt ggaaccaatt caaggtgaag
      661 gtggcgttaa tataccaccg aaaggatata ttcaagctgt gcgtcaacta tgtgataaac
      721 atcaaatatt attgattgca gatgaaattc aagttggtct tggtagaact gggaaatggt
      781 ttgctatgga atgggagcaa gtcgttccag acatttatat tttaggtaag gcattgggtg
      841 gcggcttata ccctgtatct gctgtacttg caaataatga tgtcatgcgt gttctaacac
      901 caggtacaca tggttcaaca tttggtggta accctttagc cattgcaata tcgacggcag
      961 cgcttgatgt acttaaagat gaacaactgg ttgaacgatc agaacgctta ggttcatttt
     1021 tattaaaagc gttgctacaa cttaaacatc ctagtattaa agaaattaga ggtcgtggtt
     1081 tatttatagg catagagctt aacacagatg ctgcaccttt tgtggatcaa ctgattcaac
     1141 gtggaatctt atgcaaagac acgcatcgta ctatcattcg attgtctcca cctctagtca
     1201 ttgataaaga ggaaatccat caaattgttg cagcttttca agacgttttt aaaaattaac
     1261 aattaatcat ttatatatga cataggaggg attcatgatg attaaagtag gtatcgttgg
     1321 cggtagcggt tatggcgcaa ttgaattaat tcgattgtta caaacacatc ctcatgtaac
     1381 gattgcacac atctactcac attcaaaagt agatgaaccg ttgaaattaa catttccaca
     1441 tttacaacat attatgcaac atttcgaagc acttacagtg gacaataatg actgtgatgt
     1501 aattttcttt gcgacaccag cacccgtaag taaaacatgt atccctccct tagtagaaaa
     1561 aggtattcat gttatcgatt tatctggcgc atttagaatt aagaatcgtg aaatatatga
     1621 agcatattac aaagaaactg ctgcagcaca agatgatttg aatcatgcta tttacagcat
     1681 ttcagaatgg caatcgtttg ataacaatgg aacgaagctc atttctaatc ctggctgttt
     1741 ccctacagca acattattag cattacatcc acttattagc gaaaaaatag tagatttgtc
     1801 atctattatt attgatgcta agaccggcgt gtcaggtgct ggtcgttcat tatcacaacg
     1861 tgttcatttt tcagaaatga atgaaaatct aagcgcttat gcaatcggaa accataaaca
     1921 caaaccggaa atcgagcaat atttatctat cattgcgggt caagatgtat cagtcatatt
     1981 tacaccacat ctcgtaccaa tgacacgagg tattttatca acaatatatg tcaaattatc
     2041 atctgaatat acgactgaat cattacataa attaatgacc tcttattatg ctaatcagcc
     2101 atttgtcaga attagagata ttgggacttt tccaaccaca aaagaagtac tcggtagtaa
     2161 ctactgcgat atcggcatct atgtagatga aacaacgcaa acagcaattt tagtatcagt
     2221 gattgataac cttgtcaaag gcgcaagtgg gcaagccatt caaaatttaa atatattata
     2281 tgattttgaa gtgacgactg gcctaaatca atcaccagtt tatccataag gggtgttaga
     2341 atgaaacatc aagaaacgac atcacaacaa tataactttt caattattaa acatggcgat
     2401 atcagtacac ctcaaggctt cacggctggt ggtatgcaca tcggtttacg cgctaacaaa
     2461 aaagactttg ggtggattta ctcatcgtct ttggcaagtg cagctgccgt atatacttta
     2521 aatcagttta aagctgcacc acttattgtc actgaagaca ctttacaaaa gtctaaagga
     2581 aaattacaag cacttgttgt taattcagct aatgcaaatt cttgtaccgg tcaacaaggc
     2641 atagatgatg cacgacaaac acaaacatgg gttgctcaac aacttcaaat accatctgag
     2701 catgttgctg ttgcttcaac tggggtcatt ggtgaatatt tgcctatgga taaaattaag
     2761 actgggaccg aacatattaa ggatgctaat tttgcaacgc caggtgcgtt taacgaggca
     2821 attttaacaa ctgatacctg tacaaaacat atcgctgtat cactaaaaat cgatggtaaa
     2881 accgttacaa ttggtggtag caccaaaggt tcaggtatga ttcacccaaa tatggctacc
     2941 atgcttgctt ttataacaac cgatgcatcg attgaatcga atacacttca tcaattatta
     3001 aaatcttcga ctgaccatac atttaatatg attactgttg atggcgatac aagtacaaat
     3061 gacatggtat tagtcatggc aaatcaccaa gttgaacacc aaatacttag tcaagaccat
     3121 ccacaatggg aaacatttgt tgatgcattc aattttgtct gtacattttt agctaaagct
     3181 atagccagag atggcgaagg cgcaacaaag ttaatcagcg tcaatgtgtc aggcgctaaa
     3241 tcaatatcag atgcaagaaa aatcggtaaa actatcgtaa gttcaaatct agtaaaatca
     3301 gctatttttg gcgaagatgc caattttggt cgaatcatta cagctattgg ctacagcgga
     3361 tgtgaaattg atcctaactg cacatatgtt caactgaacc aaatacctgt cgttgataaa
     3421 ggtatggctg tactatttga tgagcaagct atgtcgaata cattaactca tgaaaatgtc
     3481 acaattgacg ttcagcttgg tttaggtaac gctgcagcga ctgcatacgg ttgtgattta
     3541 tcctatgatt atgtgcgtat caacgcatca tatcgaacat aaggtggtgt tggttagatg
     3601 aaatttattg tcattaaaat tggtggcagt acacttagtg acatgcatcc atcaattatt
     3661 aacaacatta agcatttacg atcaaacaac atctacccca ttatcgttca tggcggtggc
     3721 ccatttatta atgaagcatt atcaaaccag caaatcgagc cacactttgt taatggccta
     3781 agagtgactg ataaagcaac catgaccatt actaaacaca cgctcattgc agacgttaac
     3841 actgcattag tagctcaatt taaccagcac caatgttctg caataggctt atgtggtttg
     3901 gatgcacagc tgtttgaaat tacatctttt gatcaacaat atggatatgt cggtgttccg
     3961 accgctttaa ataaggatgc tttacagtat ttatgtacta aatttgtacc tatcatcaat
     4021 tcgattggtt tcaataacca tgatggagaa ttttacaata ttaatgctga cacgcttgcc
     4081 tattttattg catcatcatt aaaagcgcct atttatgtat taagtaatat tgcaggtgta
     4141 ctcatcaatg atgttgttat acctcaattg ccattagtcg atattcatca atatattgaa
     4201 catggtgata tttatggagg tatgattccc aaagtgctag atgccaaaaa tgcgattgaa
     4261 aatggctgtc ctaaagttat cattgcatca ggaaacaagc caaatatcat tgaatctatt
     4321 tacaataatg attttgttgg cacaacaatc cttaattcat aactatgaaa ttaaggccta
     4381 acaagttttg acacgcgaga tgattccagt tcgattatcc attgcgctaa aacatttatt
     4441 taccgttcat ctcgttaaca attttgaata cagtacgata caatatgaga tgtaaaaaac
     4501 taataacctt ttacaaattt gtttatcaaa atattttaag ttttgcaaag ctttttattg
     4561 tgattatttt cacaaaatac tataatgagg atagtaaata gagaggagtc cttaagttga
     4621 cgaaacgaca aatgggtata ttcatttatg ctggaattat cggtggcttg ttatctggaa
     4681 ttgtaaaatt aggttgggag gtcatgtttc cacctcgcac accagaacgt aatgcaacga
     4741 acccacctca agagttattg caacaattag gatttagtag tgagtttacg catcaaacat
     4801 atacattttc aaatatggaa ttgccttggg taagctttat tgtccacttt agtttttcta
     4861 tcgtcattgc aattatttac tgcatattag ttaaaaaata cgcttactta gcaatgggac
     4921 aaggtgctgt ttttggtatt gctatttggg tattattcca ccttatcatt atgccaatca
     4981 tgcatactgt acctgctgtg tgggatcaac cattccaaga gcatctgtca gaattctttg
     5041 gccatatcgt ctggatgatg acaattgaat tagtgcgaca acattttgtc tatcgctata
     5101 aattaaatta atacactgac taacattaac gtgagtttta aatcatcgtt tgagtatgat
     5161 gattgatgct cacgttattt tattaactga catgatatga ttccagccaa cttacgtgag
     5221 cattaaagtc tcaaatgcgt cgtaacaaac tattattttc ggtaatttca atattgctca
     5281 gtatattttt accttatcac ttactttaat ctcgtcatga ttttgaatga tgccatcgtg
     5341 tattcacctt tcatttttcc aataaaaaaa catctaacag taaacattta ggcagtatag
     5401 tttaaaatca ctgcgcaatg atactgtcag acgtcatatt aactactcaa taactgaaat
     5461 acagacactt ttttataacc ccagggtgcc tgtcctaaga aacatacctg taccataaac
     5521 tgatcaaaaa taaattgttt gaacttcact tcacgtgatt gataaaagtg tgattgtgtc
     5581 atatcataaa tgtccaatcc tttgattaaa ccttcaccaa tcaattttgt aaaactttct
     5641 ttttgtgtcc atatttgata aaaatcattt aaactacata tttgatgtgc ttcgtttgta
     5701 gagaaacacg tcactaacgt acgccagtct aaacgttgtg atatcttttc gatatcaata
     5761 ccaactggtt ctttatcgac aacacacacg atataaggat aactatatga taagctcaca
     5821 tagatgggct gtccatcacg attgtgttga acaatatctg ccttacctcg tggcgaaatg
     5881 tgataatgcc attcatgtgg taataaacct gtgtcatgtt gaattccata ttgcactaaa
     5941 atatctccca atctgtgcat gagtttatct tgattgtatc tatagttgac tgtacgcggt
     6001 tttttatatg accaacgact ttgtgatatt aattcttcaa tacttttcaa gttactctgt
     6061 aattgcatta caaatactgt cataactttc cctacttact tattgaatat tgttttgata
     6121 tattgtgccc aatgatacag ccaattgtta gttatcgttg gccatttttc actgatgtga
     6181 ttcattattt ttaatgttaa tgttgtatct atcattgcta gttgttgttc acggtcaaca
     6241 ctagttaatc caatcgtttc gtacatgtct tgtttctgta aaatttcatt aaatgattca
     6301 tcgctgacga gttcaatttc tttgcgctta acgcattcta acaaagattt caccggcatt
     6361 ttattaggtg atagcacatg gtaaatgatt tgtggtgtgt tgacctgtgc taatgcgaca
     6421 atttgtcttg cagtcgtatc cacaaaagaa aaatctacag gcatttcagc catgctaacc
     6481 ccgatacaat ccagttgtaa caaatcattc attaccattg aaaaacggtt agtctttata
     6541 tttctcatat gccatcttcc attgtaagga ttcgtcaaat taccaacacg tacaatccga
     6601 ccatctaagc cattatttac agcttctaat acttttaatt cactataaaa tttgctccgt
     6661 gtatatggtg atgttagtag ttgcccttta tagacatccg cttctgaaaa tgtcacatct
     6721 tctgtgtcta tatcaaaata agttcccaca cttatcgtag acacatatat taaccttgca
     6781 tgatgttgtt gtgccaaacg tatgacatca acagtacctt gaacatttac tttttcaaat
     6841 tcatcatcat caccaaagtg atctgtacga gcacctgcat gaataatcgt atccatgttt
     6901 tctggtaaaa caacatcatc catacactcg aaatcaccaa caatgacttc aatgtttgat
     6961 aacattattt caaccgtctc ttctgaaaaa taatcattta aattcgtcat caacttatac
     7021 catgctattt cctcattatc agcacgtatg aaacaataaa tgcgatgact gtatccttgt
     7081 agtacttcaa tcagataagc acctaaaaaa cctgtcgcgc cagtcaatag tgtatttcct
     7141 agaggtcgat gacttagact atcctctaaa atacccaagt tataacgaga cataacaatc
     7201 ttttgtaatt ccgaaagatt atccggtaat gcaactaatg attgttgatt ttggtacata
     7261 taattaacaa tctgtcgcac ggttttatat tggtataatg tctgcattga aatatgatgg
     7321 ccaaatcgtt ttaaatgcga gacaactaac atcgcctcta atgagttacc accaagttca
     7381 aagaaatcat cgtcaacacc gacatcattt tgtttcaata cctctccaaa tacatcaaca
     7441 aatgtctgct caatttcatt agagggttcg ctatacactt tattagactg ttgtataggt
     7501 gatggatttg gcaaacgcgt agtatccacc ttgtcattcg tggttaatgg catacaatcg
     7561 atatgcgtta tagtcttagg aatcatatac ttaggcagct gatcatttaa atattgcttc
     7621 aaatcctgtt ccacttgttg ctctccgaca taataagcat tcaatatatc atgcgtatca
     7681 aagtgactta ctgttacaac acaatcagat ataccacgaa tagctaatat tgcattttca
     7741 atttcatcaa gttcaatacg gtacccgtta actttcactt gtttatctat tcttcctaaa
     7801 aattcaattt gaccatcaga tgtataacgt gctaaatcac cactatgata caactttcct
     7861 ttaccaaatg gattattttg ccatttatca gccattaatt ctggacgatt aatatatcct
     7921 atcgctaaac tatcacctgc aatacacaac tcgcctggca taccaatacc gcataacaaa
     7981 ccatctgaca taatatacac ttggatgtta gataagggtt tgccaattgg aatcgtctca
     8041 ggtatcaaat caccacaatg atgtgaccaa tacgatgtga tgactgttga ctcagatggt
     8101 ccataggcat tgaaatacgt gccacaatgc ttctcaatat atttaacaaa ggatgccgta
     8161 ctagttgccc cgcctgtaat caacttttca atataaaagt cttccataac actacacatc
     8221 tgtaacggaa tcgacgcaac cgtcacacga tgcttattaa tgagttgttg taactgttct
     8281 ggattaacac gttcctctct atctggaatc acaagcgtat gaccatttaa caaacaacaa
     8341 taaatctcca taactgatgc atcaaaaaca atatttgcat gttgcaaaaa tacttcattg
     8401 tcgcctaatt gcaattcagt tgaccatgca tgcactaaat tcaacaaatt tcgttgtcgt
     8461 atggcaaccc ctttaggcat cccggtcgta ccagatgtgt aaatagcata catctcatta
     8521 tctaacatcg ctgtgttttc aagttgattg ccatgtaaat catcatattg ttcattttcc
     8581 tttgattcaa caaagccttt agcattttcc aattgaatga ctggtaatgt cgtttcaatt
     8641 tcaacgccgt aagacatgac tgcagttact ttagcatcct ccaaaattgc accttgtcgt
     8701 ttattcggaa aatcaatatc gataggtata taagatgcac ctactttaac tgtcgccaac
     8761 atcgccgcaa tcatttcaaa actacgttct gtaaacaagg caacccgttg accattgccc
     8821 acaccatttg ataggagcat gtgcgcaatg gcatccacat agttgcgtaa tgtttcatac
     8881 gtcattgtca aatcattcat gactagcgca acatgattac cttgtcgtga gacaacttca
     8941 ttaaagtaac ttatgataga tttatttccc gggacattaa gcattcgatc gttaacatgc
     9001 gtattgaccc aatttagaag ttcctccgtg ccgtttggta tatcacaaat ttgtagtgta
     9061 tcttgatgct tcaaaatata atcaatcata atcatacatt gattacccat gtgacgaact
     9121 gtttctgagt gatataaatc ggtattatac tcgatattga ttgtatagtc atcgcgatct
     9181 tcttcaatga tgaaagataa atcaaatttc gccgtcactg atttgggttg aatgtgtgtt
     9241 aatttactat gcccaaaatg agcatgattc gtttcattgt tttgtagtac taacatgaca
     9301 tcaaataatg gattccgtga ggcatcatgt gattgatcta agtcatttac taaacattcg
     9361 aatgggtatt cttgatgctc gtatgcctcc aaactcattt ccttaacctc ttgtaaaaac
     9421 tgtgtccaca ttttatcagg tgacggttgc cctctatata ccaacgtatt agcaaacatg
     9481 cctagcattt gctccgtgcc tttatgcata cgcgcactca tcacactacc gacaacaaca
     9541 tcatcttttc gagcatatct acttaacaac gtcatgacca cactcataaa gaacataaaa
     9601 tcagtaattt gatgcttttc tacatacttt tgaagtagct gtctcatttg ttgattcatt
     9661 gtaaatgaca tcattgctcc atttgtcgtt ttaatatttg gtctaacata gtctgtcggt
     9721 aagcttaaaa taggtacttc atctttgaat tgagataacc aatattgtct atgtttcgtc
     9781 atatcacgat gcgacatcca ctcactatag tctttatatt gcaatttaag tggtaacaat
     9841 aatttatgtt gataaagtgc gttaagatca ttcattaatt gtatattact cataccgtca
     9901 ttaatgatat gatgcgtatc tataaagagg tatgcatgta agggacttct aatgtatctc
     9961 actctaattt gacttggctt ttccaaatta aaaggtgcta caaattggcg catgatttct
    10021 tgttcatccg taaaatgcgt gttaacttct tcaaagtcaa ctgcaacatc tgccacaata
    10081 cgttgtcgaa cctcatcatc tacaacaata tattgtgttc gtaaaatctc atgtcgcgct
    10141 atcaaacgct gcactgcttg tcgcaattga gctacattaa gttctgatga taaccgccat
    10201 aaaaaaggta cgttatacac cgtatcttta tggtttgatt tccataataa atacatacgc
    10261 ttttgtgcag agctcagcac ataatcatct ttaactatag tttctggaat cacttcatag
    10321 ttttgttctt gaaccttagc aatcgcttgt gctagttcaa atacagttgg cttttgtaat
    10381 aaatcaccaa tttgtaatcg tttcccagta gatgcctcta tccgattcac cactaacgtt
    10441 gcttttaatg aatggccacc taattcaaag aaattatcat gaatacctac ttgattcaca
    10501 tgtaaaatat ctgcaaaaat ttggcatagc aagtgttcgg tatctgtact cggtgctaca
    10561 taggcatccg tatcgacata gtccatgata ggcaatgcct tcttatctaa tttcccatta
    10621 atagtaatag gaatttgctc aatatgcatg aaattaactg gtatcatgta ctccggtaag
    10681 gtcatacgta attgtgattt aatcttatta tgtgataatg tatgcatcgc ttcataataa
    10741 gcaacgatat actgatcttg atcatgattt tgaacaataa caactgcttt attaatacct
    10801 tgtatacgct cgagcgcatg ctcaacctct gacaacttaa tcctaaaccc tcgaatctta
    10861 acttgtttgt cctttcgata taaataatct atgttgccat cgggtaacaa acgaacgata
    10921 tcaccacttc tatacatcag ctgatttata tttgaatctt tgataaattt atctgctgtc
    10981 aattctggct gatttaaata acctgcagct aacccaaagc cacttgtaca taattctcca
    11041 ggaataccaa cgccacaccg acgctcgcct tgcatgatat aaacatgagt acccagaatc
    11101 ggtttaccaa taggaatacg atttggaact ttgttaggta tattatacgt cgttgtaaat
    11161 gttgtatttt cagttggtcc ataaccatta ataatttgag gatgcttcgg tttttgatta
    11221 agcaaatcca cccacttagc attcaatact tctccaccaa ttaataaata ctttaacggt
    11281 accaatactt ctattcgttc actagcaatc tgattaaata atgaggaggt taaccacata
    11341 gtattaacgt cattttcatt gattaattgt tctaccgcta ttggatttaa taattgttct
    11401 tttttagcaa caatcagctt tccaccattg agcaatgcac catatatttc aaatgttgca
    11461 gcatcaaagg ctatagttcc tgataacaaa atcgtcgtct cttcatttaa tggtacataa
    11521 tgattttgat ggaccaagcg aacaatacct cggtgcggaa ttagtgtccc tttagggtta
    11581 ccagttgtcc ccgacgtgta aataacataa gcatgatctt ctaacgtgtt acatttagaa
    11641 agattatcaa tatttttcca cgctatctta ttcaaatcaa tgtgattaat attttgttta
    11701 ccattttcat ataaagcttg gtacgttatt acaactttag gcgttacatc ttttaaaatg
    11761 tactcctgac gatcacttgg atagttcgga tcaattggca cgtaagcccc accagctttc
    11821 aacacaccta tcatcgctat tatcatctca atactttttt cagctatgac agcgacacga
    11881 tcattaggtt caacaccata ctggtttctc aaacggtgtg ctaaatcatt cgcgcgtgca
    11941 ttcaatgttt gatatgttat aaacactccg tcaaattgca cagcgacatg attcggcgtt
    12001 gcttcaactt gttgctcaaa taaggtaaca actgtttgcg catcatctat ctcaggcaaa
    12061 cttaaattga tatcgtcata taattgaata tcacgttctg tcatcaaatt aagttcatct
    12121 acagttgttc gtttatttcc attttcttca gtaatttgca aataaatatt tcgaactaag
    12181 tcactcagcg tctcgattga gagcaaatca tatgctgcac tgttatatat aattttgaac
    12241 ccgtgttgat gtggaaaaat ttcaatatcc gctaaagatg tatgtgcatc atgtatttga
    12301 tgtacatcct caataacatc attacaacaa gacatcatat gatgataaca atgaaaaata
    12361 gtctctagtg aaagcgaaga cttcgcgcac tgtaattgcg acatattttg caacacacat
    12421 ttattaaaat ctgttgtaaa acgttgacat acatcttttg catcgattgt taacgttaac
    12481 ggcacaatat ttccgtgtaa atcatttggt aaatgtgatg gtacatgtat acctaatgtg
    12541 acatcatgtt gttgactcat tatatgatta gctaaataca cactaacagc caacgatgcc
    12601 atatctattg atgtcatatc atcaatcaaa tacgtttgat ataaagcttg ttcaaatgga
    12661 tgcttaattg gaaaataact atcaatatgg atgtcagagt tattctctaa ccgaaaatag
    12721 tttgagtcta atgctatatg cgatgcatct tgattgtctt tatcatcatt tctatttata
    12781 tgtgcatgct gtcgagtatt gttaataaca gtattgccac gatatgcatt gcataaatca
    12841 tcaagaaaaa tatcaatttg actatcatcg aaaatggaca catgaaaatc taatagtata
    12901 tatgcagcat cagcgaactg aaacaattta actttgaata aaggtgaatc attaaaatgg
    12961 taagtactta attcttgctt aaaaaaagct tctaaatcat agtttgcgga agaagatgga
    13021 acttgtttta tctcaataaa aggcagaaat tcatgaagta tcattgttaa attgtcatcg
    13081 gtagtaacat caaaaaaatg tcttatagat gcatgttgtt gcacaattgt cgataatgca
    13141 tacatcattt tagtagcttc aacattttta gcgagtttaa cccaatacgc attacggtgt
    13201 gtcgttgatt ctgtattatt tttgtatata cgaaaatatt cctgttgaaa tctcaaatta
    13261 cccataatca taaaaagtcc ttctttcata tcataatact cattacttac tgaaattgca
    13321 tgatgatatg ataaccgacg aaatgttaat taactcgtta tgtaatgatt aatataaaac
    13381 accattcgca acatatgagc gatatattct accctaaaat acatcttgta tcatcgttac
    13441 aattggtata tttttcaatg taaattacat acatcttcga taaatagcac actacaactc
    13501 gttaatcact ttctgttgtt cacatctcat tgcaaactca atattgttgt tacaaaatat
    13561 ccatgaagca agtttatatt aaacaaacaa ctcgcataaa acaattgtta tccttaaatt
    13621 ttaacaaatt cttaataaat ttatctctat tttaattacg accaaattaa taggttttcc
    13681 atataaaaag atgcataaaa taaatattta aataaattca atttgtattc acttgttttt
    13741 gtcccccaaa tacaccagca acaagcatgc tagcaccaat tgttaaaacg ataaacatat
    13801 acagtcccat ttgtaatgac gttaagaaaa cacccaacac aatccataac ctagctagtg
    13861 tttctgaaaa atgaatacct aatgcattaa ctgcactata tgttcctctt ttagctttag
    13921 gaataatttt aaagcgttgt tctgaaacta taggcgaata aataatttca cctacagtcg
    13981 caattatcat aaaaacaact aataagccaa actgattaag ataggttaga ccactatagc
    14041 caacaatata aatcagcaaa ccagtgatta aagctttttt aaaatctatt ttcaatacaa
    14101 ctttcgagat tgaatacgtg agtaaaatga cgacgaccgt attaatcatt agcaagattg
    14161 ctaacatctt agcacctgta atatcatatg aacctatact tattgtttca aactgatcct
    14221 ttagtctaat agcaatatat gaggagattg aaaattcacc catcatgatg atactgaacc
    14281 ccgaaatcaa taacatataa ttacggtctt tcaaaactaa tttataactg cgaaatatat
    14341 tcattatttg taatttttga taacgacttg catgcctctt gtcatcactt tgctttactt
    14401 gatttcggtc ttgaggtaac caaatatata aaataaagag tacaattaaa aatatacaag
    14461 ctgctattaa gaaaagtagt aacatactgt agccatacat caagccacct aacaatgccc
    14521 caatagctac cgataagttt gtcatccaat agctaatctt gtaaatataa tgttccacgt
    14581 cttcggtaat tgcatccata attaatgtgt ccataactgg aaattgtaat ccccaaacga
    14641 ttgtaaatat ggcatatgca acacaaaaac caataatttg ccacaattga tgtgacccaa
    14701 atacgcccat gaacacaagc attatcacca tcgtcgcttg ataaataagt actagcaact
    14761 ttttcggaaa tatctcaata aggtaaccag atataatgga caatggaaat tttagaacca
    14821 ctaaaccaac aagatatata ccgacaattg attgacttaa catatctgtt aaatatagtg
    14881 ctataaacgg tataaatgct gtcgtaataa ttagctgtaa aaaattgcta atcaatcgta
    14941 ctttcaaagt cgtacttaag cgtttcatta ataactacac ccctttccac atatatacac
    15001 actaccattt atataaaatc tattattaga ttatacgttg aaacaaagaa tttttagtta
    15061 tacaaagtta tttaacaaac aaatgcatcg actttacaat gcatcttgca tataatgcct
    15121 caaaaaaatt agcactatat gactatatat tattccaaat gaatgaaatt tatcaataat
    15181 tgcgtaagtg aacacatatg tgcgtaaatg ttaaatcatt gtcctaacat agccgtgact
    15241 atacttagat gtaatcataa aaaaactact gccatcttta caacagcagt agtgcttata
    15301 tgattcattt atataaaatt gaaagcgtta atcgctagcc cagcatcctt attctattta
    15361 gctgtataac ttttactagc aatacgacca cttgcaacaa taatatcttt atcttggaaa
    15421 ggttcatgat tgaagaaacg ctctaaaata tctttaactc catcttcaat acgtttttgt
    15481 gcttctaaag tcatacctga atagtgaacc gtcatagcat ttctaggcat tgttctccat
    15541 ggatgatcag caggtgcagg ttgtggatac caaacatcac cagcatatcc ttgtaaatgc
    15601 tcggatgcta acgcttcaac taacgcatcg cgatttacaa ttttaccacg tgcagtattc
    15661 actaaataac tgtgtttttt catacgactt aaaacatctt tatcaaataa gttatcagtt
    15721 tctggtgtta atggtgcatg aattgtaatc gcatcacttg ttgaaacaag ttcatcaaag
    15781 cttacaaatt tagacaattt atggtcttgt tgattgattg gatcatagtg ttgtaatgtt
    15841 acattaaatg gcgctaatct ttcagcaaca agttgtccaa ttcgaccaaa tccaaaaata
    15901 ccaattgttt tgtgttgtaa ttcatgcgca tgattaccta cttgagacaa gttccattca
    15961 ccttctactg attgacgatg accttcttca tagtttctaa gaagtattaa taaatccata
    16021 accgcatgtt ctgccacact aactgtatta cttcctgtaa cttcaacgac accaatattg
    16081 tgttcacttg ctgccgctaa atctacatgg tcagatccta cacctgctgt aattgctaat
    16141 ttcaagttcg gtgctttttc aatacgttca cgagtcatat atgcaggata aaatggcgca
    16201 ctaataatca catccatatc tggtaaatgt ttatctaagt cttcaccatt atctgctaat
    16261 ataatgaact catgtcctct ttcctctaaa aatgttttta atcctaatgc ttttttagta
    16321 ttaagtaatt gattttcttg accttctact gcttctggaa ataatgctac gattttcata
    16381 tccatctctc ctaaatgata aattttgact tccttaaagt acgtgttgca tgttgcttgt
    16441 tttaaaaata tgacaaaaaa acggcaccgt ttgacatacc acccccttga attattgtta
    16501 aattcaaagc gaagttagca aacagcaccg tgtaccttac accagcaaca cttattctat
    16561 tgacttatcg aaaacaaata ttgaaatgac ttcatcatca ttcaaatcaa tatctgtaaa
    16621 taattttaca aacttagcgc ctacaagact ttccatctca tttacatcta tttctttata
    16681 catctgttta atcttctctg tgcgtgtata atggagcatc gattcattgc gtttgtcgtt
    16741 taggtaaaaa ctctcaactt tactcaaaac acctgtcata tgtgcaatcg cccaattatc
    16801 tttaaacgac actcgaatac tattgggtcc tttacccacg tattctttac gataagatct
    16861 tactagattc gagaattgtt gttctttagt atgcgtatct atagtattca ccttctttaa
    16921 aataagaagt tcattaattg ttcacatttt cattatacac cttattaaca ctttttcaat
    16981 gataacgatt attattatta cgaaatgtat attttcctag ttaaaaggat ataacaaatt
    17041 aacagaaaat gtttataaac taagatttat attattttaa aaaatgtttc aacaaacgca
    17101 tctaaacaaa aaatcagctt atcacactta ccccttcaac tctgcttcaa gtttacctaa
    17161 atgtcttaat gtcggtgtca atgctgcgaa gaagaatcct tcttttaact tgcgaacttg
    17221 tggcgaatat ggtgtgtacg ctctagaacc accattgaca acagaagctt gatttacatc
    17281 taacaataaa tagccgatgt ccttcttcaa tgatattaat tcatttaaat gtgaagttaa
    17341 gttaccgtca tctaatattg cataatattc ctctctaagt tgacgataac gttttttaaa
    17401 agcttcaaca tcatactcta aatattgatt tattccgttt tgcacatttg aaaatgcatc
    17461 aattaactct aaagaacttt taattgagcc taatcctatt ggaatttggt aagcaataaa
    17521 ttgcgggcga atagttgccg caaactgctt cgcatcatgc gtgataattt gtgattgtgg
    17581 cactacgact tgattcaatg tgatttgata cgttgctgac ccgttgactc ctaaaaaatt
    17641 ggttttttca acaagagtga taccatcttg attggcacgt agaatgaaca tgacaaattc
    17701 atctgatgat tcatgtttcg aaatcgcacc aaaataatgg tcttcttgaa tattacttac
    17761 agctggcata cgtccactga caaccaattg tccatcaaca taagtgtgtt caaggttcaa
    17821 cttttctaaa tcattaaatg acttcatcgg attagacaat ccggtagcac ctaatatttc
    17881 tccagataac aattgctgtt gtaagtcatt atttaaatgt ggctgcgtgg cattttctaa
    17941 atacgttgaa aaagctaatt ggcaccataa acaaaatcct gttgtcaaac aagactgcga
    18001 tacagcttcg attacttcag cattttttct caatgccgcc tcaccgaaat aaccatctac
    18061 aaataattgc tgaataaatg ttctcggata atacgtccct tcatcaactt ctactaaatg
    18121 aggatctaat tgttctttga taagcgtttc taaagtcatc atcatctctc cttattcacc
    18181 aaatcttcta agtataaact gctcaatata actaatgaat cgatcaataa taaaaccaaa
    18241 taatccgata aagaatattg ctgctaaaac atcttctaag ttcaacatat ttcgtgcatc
    18301 aacgattaaa aaacctaatc ccgattgtgc accaatcatt tcaccagaaa ctaaaaatat
    18361 ccaacttgtt cctaccgcca tatgtatccc agccatgatt tgtttaaatg ccccgggaaa
    18421 taatatattg cgatacaatg accacccagt taaatttaaa tttgctgcta tttttaaata
    18481 ttgaggttca atgtctctaa cgcctttaat agtattgaac acaattggga aaaaagcagc
    18541 gataaaaata atcgcaatcg ctggcaaact accaatacca aaccatagaa caacaaatgg
    18601 tgcccatgct atcggagata tcggcctaat caattgaaat agcggttcga tagcgttgta
    18661 tagccaacga ttccttccaa gcaagaagcc caatggaata gcaaccaaca atgcgacaac
    18721 aaagcccgct acaaatctcc ataaactaat tgctaaatgt tggaaaattt ctccagtaac
    18781 aatgaaagac catatacttt ttcctacaag agcaggaccc ggtaacaata caggttggta
    18841 atgcccaata ataatgacca tttcccaaat gcctaagaaa ataataaatg tgataatagg
    18901 taatataaat ttgttatttg tgggacgtgt catgaacgcg atgcctcctt atacaatgac
    18961 ggttcaacaa agtcatcata tgcaggtgga ttaaacaaat gatgttgttt taccaatgtc
    19021 gtaatttctt gatagccgga tggcttaatt gttaaatcac cataggatgt ccatgccgct
    19081 gactgtgtta aaacgtcacg actttgttta aaatgatgcg tcataatgtc tacactttgc
    19141 ttgcgatcat tcattttaaa gccagacttt ttataatctt gtacaaatgc ttgcgctaca
    19201 tccttgtgtt gatcaagcaa ttcccctctc agtactagca cacagcaata cgcatcaggt
    19261 ataacgtcat caccatgttt caaagtctta cctttgccta acttttcacc cagtgcaccg
    19321 aatggttcgg ctacagaata ccctgtaatt ctgtgttcac tcaatgcggc tggcatttct
    19381 gctggcgaca tttcatgata gctaaaatgc cccggtttaa tctttaattg tttacgtaat
    19441 tcctcaagta aaagataatg tgttgaataa cgatgtggta taccaaaatg gtaatcatcg
    19501 ccattattat taaattcatt taagtgcata cctttttgtc ccataatgac attgccttca
    19561 tgatggccca atgccacagc ctttatattt gagcccttct gttttgattt catcgctagc
    19621 tctattaaag ttgatgcacc atcaatacga ccactgttta atgcgtccat taaatctggc
    19681 caattattga atttaactaa ttctagttta tatttcggat gattgtattg tgataataat
    19741 tttttagtca tcatcaaatt agctgaatgt gtaatcggca aatatccaat tttaatcact
    19801 tgctgatttt gggcattttt agaccgttct ttagacgtcc tttgccaatc acatcctgta
    19861 attataaaga ttccaatgat gacgattatg cttaaccttt tcatcgtcac tcactcctta
    19921 taaataatat tcaggttcaa cttgatgatg attcaatgca aatgtttcca taatttcatt
    19981 acgaatctta agtaggtggc tatcattacg actgcgtgga tgtgatgctg taatttcata
    20041 ttgagaaata atattgcacc cttcacctaa cagaacaatg cggtcggaaa gataaatagc
    20101 ttcatcaatg tcatgcgtca ctaaaataat agttgattgc gttttatgtt ttagttgcac
    20161 tagttgatcc tgaagtttat aacgtgtaaa tgcatctaat gcacctaatg gctcatccat
    20221 caatataacg ttaggcttat gcacatgcgc tcgacatagt gccacacgtt gtttcatacc
    20281 cccggacagt tgctcgggaa aatgctttcc cctgtcttct aaatcaacta atttaagctg
    20341 tgcgttaatc tcttcatcac taattttctg ttgtaatcca atcctaatgt tgtcattaat
    20401 cgttttccat ggcagcaaat tatgatgttg aaatagcatt aaacaatctg gagatggctg
    20461 ttgtttaatt tcgttatcaa taatgacacg accagacgat ggatgaataa atccaacgat
    20521 aatattgagt aaagtagact ttccgcaacc acttttccct atgaaagtga ctatttctcc
    20581 cttgctaatg tccaaattaa agttatgaat tactttatgt gatccaaagt gatgttgtaa
    20641 ttgttgaatt ttaatcatga caagccccct tttaatccct actattttaa taggaattaa
    20701 gtgtatatct aaagggctat gccaaacaca gcccttatat cgtgaattgt tatatctata
    20761 gatgattatg gtttggtatg agtgctctta taacataagc gttattcaat taacgacgtt
    20821 atattaattt cagctctact aaatttcaat aaaatgattg ttttagacaa tacttgcatt
    20881 tagttcatta gtttcttcat atcaactact tacatttata gcattgagct attctaatta
    20941 actgcaatcg aaatccatgc caaacctata tacatatatc tcatcaactt gttgtcttat
    21001 taaagttttt cgatgccatc aatcggtgta tcaactgctt tgcgcgcgcg acgtaccgct
    21061 tcttcatcag gtgcgttgta taaacaaata catttagaca tatcttcaca tacatatgtg
    21121 cgtttaaatt caacttctgg cacttcttca taatgaacag aatttttctt tttacgtgct
    21181 aaatattgat ccatcgtaat gccttccgga atgttccaag ttacaaggta atcaacatgt
    21241 gcatcaccat ttttaacttc atctaaatct ttaccaacta agcgtacttc tttgactaat
    21301 tgaatactaa tatctgcttc tgttaaaaat tgtttagctt ctgcttcgtc atttgcttcc
    21361 acaataaaat aaccatgagt taaattttca gtagcttgta cttcaattaa tgttggaatt
    21421 gtcttcgtag atagtgatgc tgcctttgct tctagttctt ctttcgttga tgcaaaatca
    21481 agattgttag cttctaataa aaataatgtc attaataaga cctcctataa caatggttga
    21541 aaaaatatta ctataattct tatcggatta caaggatatt aagatatttt tttaaatatt
    21601 tagataaaat tctgtaaaat atattttcaa agagtataaa aatcatgtta gggtataagt
    21661 aaataaacat atatataggg ggctatcatt atggataaag aatctgtcgt agcaagttta
    21721 gctagaaaca aaaagattgc tgtagaaaca atggccggtc aaaggtacat catagaacgc
    21781 attttacata caaatgatga aaaacatatt catattttga aacctaaaga tgtcgtgtta
    21841 gatgtagata gcatcaaaga aattgacgaa aatcatttaa atgatgcaac ataagtataa
    21901 aacgaaacgt gcgcctatga catagattaa tgttttaagc gctacaatgt tttatttatg
    21961 ttaatttgtc gaagtatggc tcatctaatg gattcaattg caagttttca atttgcaacg
    22021 ttgaatgtgc aatgccatat ttttctttca acaatgatga tacttgatca atcgcttgat
    22081 aatcatcacc ctcatatttt ttatctaaca caacatgggc acttaatgaa taatgctctg
    22141 ttgtaatact ccacaaatga aattcatgta catctaatat gccatctatg tttttaatat
    22201 ctgccataat ttgatcagta tccaaatgtt gaggcacact ttccattaaa attaaccacg
    22261 cattacgcgt aattttataa ccaccacgta aaatgatgag tgaaattaca atactaatga
    22321 ttgggtcgat gatgcgccat cctgtaaagt aaatcaatac aactgcaacg atgacaccaa
    22381 tagagttcaa taagtctccc atgaaatgcc ataatgcact ttgaatattg atattgtctt
    22441 cttgttttaa agaccttaca aggataacag tcaaaataat attgacgagt aaaccaatac
    22501 tagcaatcat aaacataatg ccactttcaa ttggttgcgg ataaataata cgtacaatag
    22561 cttcatataa aatccagatt gaaattacaa ttaatgctaa accatttaaa aatgcagcta
    22621 atatctcaaa tcttaaatat ccaaatgtgt atcgtgcagt cggcttttta cttgcaaaat
    22681 aaatggccaa catagataaa ccaagtgcta atacatcact aagcatatga aatgaatctg
    22741 acagtaatgc caatgaatta gatactaaac ctccgacaaa ttcaatcact gtaaataaca
    22801 atgtgatgat tagtgatgcc cacagcgtct ttttagaact ttgttgaatt ttttgatgat
    22861 tgacatgatg aaaatatttg tatctgttta tcgtattcgc ctttttccca ttcatacgtt
    22921 tgccactcct aaataaatgc gtcgtattta atattgtcgc ttttaaaaat aattctttat
    22981 agcttctcag gttaatatta atgcttccgt aattaaccat gatttataca tcatcgtcat
    23041 ttacatgaca actcattaat ctttaccctt tatagcttta tgtaatatca ccttgtgtaa
    23101 tatcaccttg gaaaactttt acagcacact cgcgctttgc tttattccta tcatatagaa
    23161 aattttaata atgtacgaga ttattgtgtt agttacataa aaatgacaaa aaagtatctc
    23221 aaatttattg tttagtttca ttaagttcaa aaaatcgcaa taactttaat gattgttccg
    23281 catattaaaa caaacgatga ctttgcatat tgaacttcat atttctaaat caccataatt
    23341 aataaacaac tctgtcctta taatagccgc atcgcaaatt agcataatta aaaacagcag
    23401 taagatgatt tgcaattaaa aaccatccta ctgctattat tttagtcatt atatcctaat
    23461 atcatgatac gtgttcctat atattttgtt ttgcttaaaa tctaaacaac gccgttaaat
    23521 gttaagactt accgctagac tgacctacaa acacgcttca gaaacgatat ttattctagt
    23581 acaaaccttt taaagcattg cttgtatcaa tataaatatt tttaacttgt tgatagttac
    23641 ttaacgcacc tttataagtt tctcgaccga tacctgattt tttataacca ccaaatggtg
    23701 cgccttctgg tacttggttg taagtgttaa tccaaatacg tcctgtacgt acagctttag
    23761 caatatttaa tgcacgtgtg atattttgag aaaatacacc gcctgctaaa ccatactcag
    23821 aatcattagc tatatcaatt gcttcttgat cgtccttcac tttaatcact gttaacactg
    23881 gtccaaatat ttcttcttgt gctaatttgt gatgattgtc tggcacagca attaatgtcg
    23941 gctcaaagaa gaacccttta tctaatccat tttcagttaa gcgatgaccg cctgctaaaa
    24001 tttgtgcatc tgattctttt gctgcatcaa tatatgattg aattttatct aattgatcct
    24061 taccagtttg actacccatt tgtgtagctt catcttgtgg atttccaact ttaatatttg
    24121 aaaatgcctc ttgtaaacgt ggcaccaatt gatcataaat tttttcatga actaataatc
    24181 gagaacctgc actacatact tcaccttggt tgaataaaat acctaactga ataccttcaa
    24241 ctgcaaggtc taaattagca tcatctaata tgatattggc gcttttacca ccaagctcta
    24301 atgtagcggg tactagatgt tttgctgcag cttcggcaac ttgataacct acatcagttg
    24361 agcccgtaaa tgataattta tctacaccat catgattgaa aattgcatta cctgattctg
    24421 aacctttacc cgttagtata ttgacaacac ctttaggtaa tacctcttgg aaaattttag
    24481 caacttccaa taaacttaat ggtgttgaag acgaaggttg aatcacaatt gtattacctg
    24541 cagcaatggc tggcgcaatc ttccatgcag ctaatagcat tgggaagttc caagcaacaa
    24601 cagcacctac gacgccaatc ggctcatgtc gtacgatact cattgtgtct ttatcgatat
    24661 cattcactgt accttcttct gtttcaataa cacttgcgaa ataatggaaa tgtcttgcag
    24721 caaatggaat atcaattgct gttgtctcac gaatcggttt accattattt aatgtttcaa
    24781 tcattgcaat tttatctttt tgtgccatta atttatcacc aatatcacgc aacatttgtg
    24841 cacgttctga tttagaagtt aatgaccatg attcaaatgc ctcttgcgcc actttgactg
    24901 catgatcgac atctttatct tttgctcttg taatatgtga tagtgtttct ccagttgctg
    24961 gattagtcac ttcgattgtt tcgtcactgc tacctttaac aaattcccca ttgataaata
    25021 aaccataatt ctctgcaata taatctcgaa cgtttactgc catattatct ctcctttgtt
    25081 tattggctat aatataaatc tcttagcatc atatttaata atgcttttaa acgatatgat
    25141 tcacattata tagtaagcgt ttacatttta taaaataatc agctcacaga agtctcattt
    25201 ttaaaacttt tgcgtcgttt ttgcacataa aaataatctt attttcgaaa acatattgca
    25261 cttttcaaaa ataaaacaca taatataact ataatacttg agaataattc tcattaaata
    25321 gtttggttgt tatgaaagtc gcgcttaaaa tgcgttaatg tgacaaggat aattcaacat
    25381 cgtttcattt tactgagtca ttgcacttat catacacatt atatttagca tgagttatat
    25441 tactaaaaca atattacatg cgcattagta ctagaatgga gttttttcaa tgcgacttat
    25501 tttaatcgtc attggcttaa tatttacagc gcttggtatt gcaggtgccg tattaccttt
    25561 actgccaacg accccttttt tactcgtagc agttttttgt tttgctcgaa gttcagatcg
    25621 cttttacaat tggcttatta atcaaaaaat ttataaagaa tatgtagaaa acttttattt
    25681 acatcgaggc tacacgctac aacagaaaat taaaatttta attagcttat acattgtaat
    25741 aggtttttca atttatatgg tggatgttct tgcagtccgt gtaggattaa tcataatggt
    25801 tatcatacaa accgctgtac tctttacatt tgtaaaaaca ttacccaaat caaatcataa
    25861 aatagaggag tgattgccca tgtttatggc agaaaataga ttacaattac aaaaaggcag
    25921 tgcggaagaa acgattgaac gtttttacaa tagacaaggt attgaaacta ttgaaggctt
    25981 ccaacaaatg tttgtcacta aaacattaaa taccgaggat acagacgaag ttaaaatctt
    26041 aactgtttgg gaatctgaag atagctttaa taattggttg aattccgatg tatttaaaga
    26101 agctcataaa aatgtacgtt taaaaagtga tgacgatgga cagcaaagtc caatattatc
    26161 aaataaagta ttcaaatatg atattggcta ccactatcaa aaataaatat attaaaaacc
    26221 cctagtcacc gctggttgtc taaaccaact tcatgattag gggtttttta ttatttgtga
    26281 cgtaaaggta cgaattcatc cggcttgtct gtgcgcaatc caaaataata ttctattgct
    26341 tcacaaatac gtcgtgatgc ttgtccatca ccatatggat tattcgcttg actcatacgt
    26401 tgatacgctt catcatcctc aatcaattgt ttcacatttc gaacaatatt gtcataatct
    26461 gtgccaatta ctctcgatgt tcccgcctca acgccttcgg gacgctctgt atgattcctt
    26521 aataccaaca caggttttcc aaatgtagga gcctcctctt gaataccacc agaatctgtc
    26581 agcacgaggt acgattgatt tgtaaaatta tggaactcaa tcgcatctaa tggctcaatt
    26641 aattcaatcc gatttctccc agataaatat ttttcggcaa tcgctcttac ctttggatta
    26701 cgatgcattg gataaatgaa gacaacatct ttatattcat ctgccaaatc tcttactgct
    26761 ttaaaaatct gatgcatcgg ttccccaata ttttcacgac gatgcgctgt tagtaaaaca
    26821 actttcttgc ctttatgttt attaataatc gttgaaacaa aatcattttg aactgttgtt
    26881 gacaatgcgt caataactgt atttccagta ataaagatac gctctttgtc tttgttttca
    26941 aaaagtaaat ttttagctgc aattactgtt ggcgcaaaat tcaattcagc aatattactt
    27001 accatgactc gatttaactc ttcaggaaat ggtgagtatt tctgatgtgt tcgaagtcca
    27061 gcttctacat gtccgaccgg aatttgatga taaaatgctg ccaaacttcc tacaaaagtc
    27121 gttgtagtat caccatgtac taaaatcata tccggttgtt cctcattaat gatgctatca
    27181 agtttagcaa gtgcattcgc cgtaaggcct gctaatgttt gttgatcttg cataatattt
    27241 aaatcatgat cagcttgaat atcaaatata cttaacacac tatctaacat atctctatgt
    27301 tgtgctgtaa tcacaatgtt cgcttcaaag ttcccattat gatcaatttc ttttactaat
    27361 ggtgccattt ttattgcttc gggtctcgta ccgaaaataa ccataatttt tttcataaca
    27421 ttatcctctc tgaagttcaa acacatgaca gcttatcttt ccttaatcat gtatgcccta
    27481 gtttgacaca ttttatttgt cgataaaatt aaatatattg ccataattat aatacgatac
    27541 atcttcaaat gatgatttca caacattttt tgtatcaaaa atcactttat gcttcatttt
    27601 atcaaaatga ctgtccgata aatttttaaa ttctgagtgg tcacttaaaa tcaatactag
    27661 cgatgcgtct ttgacagcat gtgacatatc atgttccaca aaatctaatt caacatgtgg
    27721 atcataagca catacttcta tgtctggttc ttgatttaat agctcataaa tatcaaatgc
    27781 tggtgattct cttatatcat caacatcacc tttataagtt aaaccaaata ctgtgacttt
    27841 attcccgctc aacactttga tgatttgctt cgttgtatca acaacatagg ccggcattga
    27901 attattaatt tcacgtccag tttgaattaa ctttgcattt tcagggtctt tagcaataat
    27961 aaagtacgga tcaacagcta aacaatgacc gcctacacct ggaccaggct gatggatgtt
    28021 aacacgcgga tgtttgtttg ccatttcaat cacatctaat acattaatat ttaagttatt
    28081 gcaaattttt gttaattcat tagctaaagc aatgttcacg tctctatatg tgttttccat
    28141 tagcttactc atttcagcag tacgtgcatc tgtttcaatc atttctccct gaacgaatgt
    28201 gcgatagaca cgtttacccg cttcaataca agcttcagtc acaccgccaa tgatacgatt
    28261 gttatgaact aattcttcta aaatttttcc tggcagtaca cgttctggac aatgcactaa
    28321 ataaatatct tcacctattg taaaccctaa attttcaatg actggtttta caaaatcatc
    28381 catcgtttta ggcgcaattg tcgactctac aataatggta tttccttttt ctaaaaatga
    28441 taaaatacta tctaatgcac gcataactag cgaaatgtca catgaccggt actgatcatc
    28501 attattcggc gtcggaacgg caatgataaa aacatcagat gcatctggcg ttgtagatac
    28561 cttcaatttt cccgatgaca gtacctcttc ataaacctct tgtaatccag gttcttcaat
    28621 actaatttga ccactttgta acttatcaat cgtttgctga ttaatatcaa caccaagcac
    28681 atcgacgcca tgttttgcaa acataattga tgttggtaaa ccaatataac ctaagccaac
    28741 tactgttaac ttcatactat aacctccaaa taaaacgatt tgtatgttga ttttatttaa
    28801 tagatgactt atgccttatc tttggttgtc gtttcatcca tgatgtccgc aataacaagt
    28861 gacatttttc caggaataat ttcaagtgca ttattatttt catataacgt attgctgtat
    28921 attaaattgc cgaataattt tctaaagacc gacaacttat taaaatactt atttagcatt
    28981 gaaggcatgt tgatcaatac cgttttacga tgtgattggc gacgtatttc atacattact
    29041 gacgatgtat caaagtaaaa actatcttga ggatggtaca cacctgtcac ttctaatgat
    29101 attaattgat caataaatgc tgtcagatgt ttaatatata atgcactgcg ctgattgtta
    29161 atattgggaa tgattggcaa tcgctttgac aattgcatta accgttggaa atttcctggg
    29221 caatgtgcac cataaatcat tggtggtctc acaattgcta ctttaaacga atcactaatc
    29281 aattcttgta atgcttgttc agcgaacttt ttggaaatac catagttggt cgtagggttc
    29341 attggtgttt gtgtatcaac ttgatctgat ttaccaacat gaccttcttt tccataaact
    29401 gccatagtac tcataaaaat aaattgttta acgtcttcag ctttagcctt ttgtgccaat
    29461 tgtttcgtca gcaacatatt cacttgcata taatcagata gccttgcttg aggtgaattg
    29521 ttgtgaacca aagctgctgt atgaattaaa acatcataat ctttgaacga ggtcgacttc
    29581 cataattgat tcctaacatt aatttgatct acttgatgtc cttgttcaat aagcttatct
    29641 tttaaagcat taccgatata tccatgtacg cctgtaatta aaatattttt tctcattagt
    29701 gatgcacacc ttctgaagta acgatatttt taattgtttt atatatgata tacatatcaa
    29761 gcatcataga ttgatgtgtt aagtaataat gatcatacgc tactttttga tcatcagtga
    29821 tatcatctct ccccatcact tgagctagtc ctgtcacacc tggtctaatc gtatgcacgt
    29881 tcgcttttgt acgtttttcg attaattcgt attgattata aagcgctggt ctaggaccta
    29941 caattgacat ttctcctttt aaaacattca ataattgtgg caattcatca atagaggtct
    30001 tacgaatgac cttccctgtc tttgttatat acgatgttga atccattaaa tcagttgcaa
    30061 cattaggtgt gtctattttc attgatctaa acttataaat attaaacaat tcattattaa
    30121 tcgtcggtct tttttgtttg aaaatggctg gtccaggtga ttccatttta attagtaatg
    30181 ctgtaattaa cagaatcgga cttaaaacta ctaaaccata tattgaactc actacatcga
    30241 ataatcgctt cataattatc tcctcacttc aacagattgt aatacttcat agtattaatt
    30301 tctctcgtat atttatcttt aaacagttga tgtgcgcgct cacccatctc gtgcaattcc
    30361 ttactcgagt gagtgttgat gaagttataa atggcatgtg catcgccatt atcaatttgg
    30421 atacccgcat catattgttc aacatgttga acgatatcag attgcttatc catgatgagt
    30481 accaacgctt tcttagctgc aagatagcca taattcttgc tcggcacgcc taaaccgacg
    30541 ccttctttaa tcagcgatgc aatacataca tccgcaattt ttaatacgtc agcatagtct
    30601 gtacctgtta aaaactcaaa cattttaaca ttttcaatac gatggtcttc tattgccgtt
    30661 ttgacatctg caaatttctt accatgacca caaagtattg ttaacgtttg agactgatcc
    30721 ttatttaatt ttaaaaatga gataagtgtc tccatatcct gtaactgccc catattaccg
    30781 ctatacaata aaattttgtc gtattgctca cggtaagctt taaatgtgtc attatagata
    30841 cgattgtctt gtaattgacg catgtcatac cagttaggaa tcacatggat attgtcagca
    30901 tttttagaaa tttgatgatt tagtaagtag tttttcattt ccgtaccaag gacaatgaca
    30961 ttttcagcat tcttgtagac atgtctatta atgtaacgca tcagcttatc aatcatgcta
    31021 cctggacgag ttgcacctgt cttaatcgca ttatcaggtg ctatatcata caccacaaaa
    31081 gaatattttt tcttaagcag tctgtgtaaa acgtctggta ttaatggcaa gattggtgga
    31141 ttagagtaaa caagaatctg atcatatttc aacattttag gtatattaat cacgaatttt
    31201 gaaaataaac taaagaaatt gatgatcctt ccaaccttac ttttgttatt aaacctcgaa
    31261 tacttgagac gtcgaatgcg aataccacga tgcatctcgg ttttagaaac ctgtttatga
    31321 ttactatatt catatggcca tccacacatg acatcgacat taatgtgatt cgcaattaaa
    31381 tcttccgcca attgagttgg taacgtcgca gaagatacat attccggata aaaatactga
    31441 cataaaatca aaatcttttt ttcactcatc taatcgacgt cctttttatt aatgaattta
    31501 gactttaaac ctataatatt taaaatcaca tacttaataa tgttgctgaa cagcgttgtc
    31561 caaaagaatc catttagacc aaacgcaatt gtcattaaaa tagttatgaa tataaatgta
    31621 atcgtgtgaa gcgtcatata attcgcttgt aatgttattg atgcgtgttt tgtatttaaa
    31681 gtttggatca taatactcac tgcattgaat aaaacaccta tattacctaa aataataaac
    31741 ttcgaatata aacttgaatc aatgttatac agtaaagaga caataattat tgtaattgga
    31801 taacatataa tcatgactaa acatagggca gctatagcaa ttagattagt tttcaaatat
    31861 tgcttcttta tattgtcact ttcatttaca gaaatatatg aaagtactac attattaatc
    31921 ggatatagaa atgtagctaa cattttccca ataaatgttg aaagaaatga tatagttaca
    31981 gctgttccac ctataattgg taataagatt aatctatcta aatagagatt caaattatta
    32041 aggctatttg tactcagtag catcacataa tcttttacca cattattatc ttcactttga
    32101 tactcgccta tagttaatcc ccgtaattta accaatgtat atatcgttgc aaacaattca
    32161 ctggtaataa aacaaacaat ccagttttgg gttaaataat atagaaatag tcctatcagc
    32221 aaacctaaaa attgaataag agcaatatac aaaatctgat tatattttaa agtcatccta
    32281 aaaaatacat tcagataaat ccttaaacac attaaaatat taagtagaat taaaaagata
    32341 atatcgatga tgttcaaatt aaaaaagtaa agaaatacaa taattaaagc tatactctca
    32401 atcagaattg agattaaaag tatcgatgca aatttccagt agtaatgatt ggatttgtat
    32461 agattcatat taatcaatcg tatattgtta agcgtattgc ctaatacaac actcgtgatt
    32521 gttattattg tataaatcgt taaaatagaa ccgaacgctt cattacctac tcgttgatta
    32581 ataattggat aagctaaaaa ttgtaatcct aaagctataa gcaacgtacc aattattgtc
    32641 tttacgctat ccataataaa aactttctta gccatgatga cgccgtcctt ttgtaataaa
    32701 atagaacaac acaaaccaaa attcacttac aactaaataa tttgaactaa acattaataa
    32761 tgtgattgga tagattgcta taaacataac gagtaaatct atagtgtttg gatcatagtt
    32821 cctaatcatt ttataaacta gtagcaaaat gcaaatcatt atgataaaaa accctaataa
    32881 gccaaatgat agaatcaact caataatgat gttatgtggt atatttccga ttagtttata
    32941 atagttaaat ggcccatagc ctaataacga actttgttga ataaagtaaa tacctttttc
    33001 ataaatcggt cctcttccag aagtaccttc taaattaagt gttccacctt gtagatatga
    33061 aaatgttcta gtattcgaac cttttgtaaa aagaaagtaa atcaatacac tagatatgct
    33121 taatgcaaaa atatacataa tgctttttac tgcgataggt attcctcttt taaacgtaat
    33181 aagtataaat gcaaataagc cgtaaagaat taataaaata gcacctccac gccctcctgg
    33241 tataaacaca atagggatat caattattgt aaatagaaca tatatccact tatgtttaac
    33301 tgaacctttc ataatgaaat aaatgcctaa tccggcagta aatgctgaaa ggtacgaagc
    33361 gttttgatag ttcataagtc caaaattgat atagctaggt atctcacctg taagttttgg
    33421 tattaaaatt acaaaaataa atgaaacaga aaatatgaaa aatactaatt taaaaaatct
    33481 ttctaccgta gccttgttta tatatttaat ataaatacca ctaattgccg ctggaactgc
    33541 ccatgttaaa aagaatagaa tattattttt agctagtttc tcttccttat ctggtgaaaa
    33601 ataataaaaa gctagataaa gcaaacatat agcaattaat aatattaacc ctcgcggaat
    33661 ttcttgggtg acaattatct tataaatagc aaacaccgta gtaattaatg ctataccaac
    33721 catggttgag taatacaccg gctctatagg gaaccctaat acttctttag taaatgtaga
    33781 gattactata aatatgttca tgctgataat tgcacaaagt acaaaaaatt tcatagtcta
    33841 cctctcttta aaatttatat aattctacta accgttcact ctctatttgc caattcaaaa
    33901 ttttagacgc cttaattgca ttttgacgta aatgattaaa caaatcgtga ttatctctta
    33961 attttctaac cgccttttca atttctaacg gcgtaacttc ctttagaaca atgccaaatt
    34021 tatatttttc attgagataa atatgctctt tgacaggaga taaaattact ggtaaaccag
    34081 catgtataca ttcaaaaatt ttattagata ctgtatattc aaaattaata gatactggtt
    34141 tcgtcaagac aacaccaaca ttactttctg ctaacttatc aaccaattct tttacttcaa
    34201 ctggtttatc caacctaata ttttccgggt tataactaat cagttctttt atcacttctt
    34261 catgcggacc aaaccctcga attatgaatg aaggagcatt ttgtttaaaa gctgatgaag
    34321 caataataaa ctcttcatat cctctgtcca ttacaatttg accttgatat acaatttctt
    34381 taaagttttc gatttcttta aattctctgc tatcatttaa aataggtgca ttcgtaataa
    34441 cattcgcttc cttcttatat cctttagatt gataatattc ttttgctgca tgacttactg
    34501 ttacgaaggc attaacacga tgttttacta tgtgtttttc tatactttct acaaactttg
    34561 aaataagtgg aactttatta ataaaggcat ttttcgcata tatttcatgc gcatcataaa
    34621 caatattagc ttttttataa ttgcttaaat agaccattaa taatacgtcg aaatcatttg
    34681 catgaatcac gtcaggttta taagctttaa tttctcggat aacacctgtt gcaaatctta
    34741 tacgcttaat taatttagaa agaatatttt ttggatctac cttgctacct aacaaacgat
    34801 aattacaatc taaattttcc aatcgcttat tagtagcttg tgaattattc attccaacaa
    34861 ttttataatc atccgtaacg cctttaattg tttctatttg tttaagtacc cttgggtctt
    34921 gaacaatatt actcgataca atatttaaaa ttctcatatc attaagtatc ctttttattt
    34981 aaatattcaa ccgaaaatcc ttttagtttg tcaggcgttt tctcccacca cttgctttcc
    35041 aaaagttttt caattgtttt attgtcaaat cgcttcttaa tcacttttgc aggaacacca
    35101 ccaacaacct catatgctcc tacattttta gtaacaactg agccggctgc tatgactgca
    35161 ccagtattta ttgttaaacc atccataata attacatttg caccaatcca cacatcattt
    35221 ttaattgttg tacggcttgg ttggtcatta aagtctataa acttttgctt tatgttaaat
    35281 ggattattat tagaataaaa aatcggtgat gagctaaaaa agtgtgtagg atgttttcct
    35341 aacccaattt ttacatccga agatatcgaa caatatcttc ctacttctac attattaaaa
    35401 tcactaccaa atccaatata actgtattca ccaatgtgag aattcctgat tttacaccat
    35461 ctatctatat agttattgcc atcaaatttt gagtttgtaa tatacgccaa gcgatgaatc
    35521 ttaacattcg attctttaga ggactggttt ttcagcaaac caattatctt ttcaatcgct
    35581 atcctcatcg tcatttcctc caagtatttc gattgataat atctttatag ctttgaataa
    35641 ttttaactac ctttgtcgaa acgttagtgt ctttataatc aatagcatca atcatcggtt
    35701 cgttattgtt ttgcatctct cttgctagtt caacggattg gattagattg ttataggtaa
    35761 taccacctac aataaccgta cctttatcta gtacttccgg tctttctgtg gaagttcgaa
    35821 taaggacacc agggaacttc aaaatagacg actcttctga caatgttcca ctatctgata
    35881 gcacaacaaa tgcatctttt tgcaatgcat tataatcaaa gaaaccaaat ggctttaact
    35941 gtttaactaa tggatcaaat tcaaatttac tttcttcaat tttcttccaa cttcttggat
    36001 gcgttgaata aatcacaggc attttatact ttttggcaat atcatttatc gcattcatta
    36061 atgatttaaa attcttttca ttatcgatat tctcttctct atgcgcagat actaaaatgt
    36121 attgttgcgg ttctaatcct agtttattta aaacgtcact gtgattaatt ttatctcgat
    36181 gcgcttctat cacttctgtc atcggtgatc ctgtcacaaa gatattcgct ttattgaagc
    36241 cttcatctaa taaataacgt ctgctatgtt ccgtataagg tagattcaca tcactgacat
    36301 ggtcaacaat tttacgattg atttcttcag gtacattctg atcaaagcat ctattacccg
    36361 cttccatgtg gaacacagga atctttaatc gtttagcaga tactgctgct aaacaactat
    36421 ttgtatcacc aagaattaaa agtgcatctg gttgttcgcg taataaaaca tcatatgtct
    36481 tcgcaataat attccccatc gtttctccaa ggttacttcc aactgcctct aagtagtggt
    36541 ccggttgtct taattccaaa tcatcaaaga aaatttgatt caatgtataa tcataatttt
    36601 gaccagtgtg tactaatatc tgattaaaat attgatcaca tgctttaatc gttgatgata
    36661 aacgaatgat ttcaggcctt gtaccaacta ttgtcattaa tttcagtttt tccatgcgct
    36721 atacctccaa gaaatacgta tctggctgat ttggatcaaa catttcattc acccacataa
    36781 tagttaccat atctgtgtcg cctaaatttt caatattatg tgtgtatcct actggtatgt
    36841 ctacaacttc taatttgtcg ccagaaacat aatattcaat gatttcatca tcattaacat
    36901 gtctaaaacg aattacccct ttacctgata cgactagaaa tttttcgttt ttagtatgat
    36961 gccagtgatt acctttagta atacctggtt tagaaatatt tacagaaact tgaccacgat
    37021 ccggtgtttt tataaattct gtaaaagaac ccctatcatc cacattcata agtaagggat
    37081 aactaaagtc tgtactaggt agatagctta aatacgtact atacaaatct ttttcaaaca
    37141 agttatctaa tttcggcaat gttcgatcga gacgtgactg tttgaacttg tataataaat
    37201 ctacaatttc tcccaatgtc actttaaata cgtttggtac tgtaggtaca ccattttcaa
    37261 tcgttggagt tccttcaata gcacgcttta tttcagcgac gatatcatcc acgtagttta
    37321 gcgttagttc aacattccga tcattaactt gaatctcttc gttacgtgca attttgtaac
    37381 aaaatgttgc tatcactgag ttataattcg gcttacacca cttgccgaat aaatttggcc
    37441 agcgataaat ataaaccgta ttgccatact cttcggcata ctctcttaat agctgttccc
    37501 cttgcaactt actctcacca taaggattat cttgtgttgc ttgtattgaa gacgataata
    37561 atatcgctgg ctttttcgta tttctagtta atatatcaag tacatgatct aaataactca
    37621 cgtttcctaa gctgaattct ttgtcatgtt caggtcgatt aacacccgct aaatgcacga
    37681 caaagtctgc tttcaacaat gctgactcta attcttcctc tttagtttgt cgatgtactt
    37741 cgaaaatatg atgatctgtc gttgaagtta aatctgcttt caagtttttt cctacaaaac
    37801 cttttgctcc tgtaattaca atattcaacg tcaatctctc ctatctcatt gaagctttat
    37861 aatcattcaa ttcgtttcta acatattcta gtgttaaaag tttttctttt atctcttcca
    37921 ccgttaaaat atgtgtatta tcggagttat attcataaga ttgcgtaatc ttttcgttac
    37981 cggtttcaac ataattacta taatttaaat ctctggagtc tgccggcact ctaaaataat
    38041 cacccatatc ttcacattgt gcgtattctt ctctcgtcaa caatgtttct gcttttttct
    38101 ctccatgtcg cgtaccaatg atttcaattg cattatcagc ttcaaacaat tctaataatg
    38161 cggtcgcaag atcccctacc gttgagcttg gtgctttttg aaccataata tctcctgtct
    38221 ctgcatgctt aaatgcatga acaactagtt ctaccgcatc ttctaagctc attaaaaatc
    38281 ttgtcatatc aggatctgta atcgttaaag gttctccagc tttgattttg tcgataaaca
    38341 aaggtattac tgatcctctt gaagccatca cattaccgta tcttgtacca caaataagcg
    38401 tttgttcact acgaatattt cttgatttgg ctacgaatac tttttccatc attgcttttg
    38461 aaatacccat agcattaata ggatacgctg ccttatctgt agataaacat atgacttttt
    38521 taacattttg atgaatagca ctttgtaaga cattttctgt accaataata tttgtcttca
    38581 ctgcctcaac tggaaagaat tcacatgacg gcacttgttt taaagctgct gcatggaata
    38641 cgtaatcaac atctcgcatt gctgtttcta cactttgact atcacgcaca tcaccaatgt
    38701 agaactttaa ttttgaatta ttatattttt ttcgaatgtc atcttgtttt ttctcatcgc
    38761 gtgaaaaaat acgaatttct ttaatattag aatctaaaaa ctgtttcata acagcattac
    38821 cgaatgatcc tgtgccccca gtaattaata aaattttgtc atcgaacata attatctccc
    38881 tttttgccat tggcatagtt aataattttt tctttactaa agtcattgac gatgtcttga
    38941 ataatcactt caacttcatt acatttcata tgttgtactt tgccacgata aattttttca
    39001 aatacttgtt caggatgaac ctcatcttta ttcataagct cttcaaacat tttttcgccg
    39061 ggtctaatcc ctgtataagt aatgcgtatg tcgtcttctt ttttaccact tagcttaatt
    39121 aaattacgtg ccaaatctac aattttcact ggttctccca tatctagcac aaatacttcg
    39181 ccaccttctg ctaatgcccc tgcctgcaaa actagtctag aagcttcagg aattgtcata
    39241 aagtaacgtg tcatttcagg atgtgtcaca gtaactggcc caccttcttc aatttgactt
    39301 ttgaaaagtg gaatcacaga tcctctcgat ccaagtacat taccaaatct cactgcaaca
    39361 aaatttgttc gatgcgtttc atcatttaaa ctttgaataa tcatttctgc aattcgcttt
    39421 gaagctccca tgacattagg cggattaacg gctttatccg tagaaatcat aacgaatttc
    39481 tttacctctg catttttagc agcttcagca gtatttttcg tacctaaaat attattacgt
    39541 actgcttctt cagggttgtc ttccattaac ggcacgtgct tgtgtgctgc tgcatgataa
    39601 actgcgtatg gtttatacgt ttccataatt tcaaacatac gcgctctatt ttgcacatcc
    39661 gctataatag gaacgatatc aacatttttt ccgaagcgat ttcgcaattc acgattgatt
    39721 aaataaatac tgttttcacc atggccaagt agaataatac gttctggata gaaattacaa
    39781 acttgtctac aaatttctga tcctattgaa ccacctgcac ccgtaactaa aatagtttta
    39841 ttcgtcaatt catttgatat catatccata tctaattcaa caggatctct gcctagtaaa
    39901 tcttctactt caactttttt aagttggttc acttctaact caccagacat gacgtcttct
    39961 atatttagca ttttcaataa ctcaacgcca tccatatggc aaatattatt aatttctttc
    40021 aaacgctctt gaccaatagt tggaattgca atgatgattt ttttaatctt atatttcctc
    40081 actagttctg gaatatccgc aattttacct tggactttta caccctcagt aattgtgata
    40141 ttgcgtttat gttcgtcatc atcgactgct aataccggtt caagtttcat ttcgtcactt
    40201 ttcaacattt gtctaatcag cattgaacct gcttgaccag caccaacaac taaagttggc
    40261 ttcttattaa atgacttacc tccaaggtat ttccgataaa tacgccaaaa taaccttgag
    40321 ccacctatta aaatcaagtg catcatccaa gtaattaaat acaatctaaa aaacggtcta
    40381 ttgcctgtaa caattgtcac gaccaccatc gtaataacga tagatgtcgt cacagcttta
    40441 acaattaaaa tcaattcact cacactggca tattcccacg ctcgatgata catattaaaa
    40501 ataaatgctg aaatatgatg cgatatgaat agtgatatag ctgccaatat taataatttg
    40561 acagaatatg ttttgaaata cggttctaaa atgtaataac ttacgaatac tgaaaatgtc
    40621 actatcagtg aatcgattaa tgctagtatt aaaagccgca atttcacaga taaatgtgcc
    40681 ataaaacccc tcatttgctg tttataaccc aaaccatctt ttctgtttat aatcttgttg
    40741 tggcattcgt ttaggaattt ttttatcatc aacaactaac ttcgcattac taataaatcc
    40801 gttcatatct tcataataat cacgtaattt cttatcatta aataagtctt tcattaagaa
    40861 cggtctgatt tctgtgttat gcgcatctga accgatgaaa tgtgtcagat tgttttcaat
    40921 catttgaatt gctaattttc taattttttt accggaaata cccgctaatg acgccgttgt
    40981 cacttgactt aaagcacctt tgttaattaa atcgtatagt atgtcaaggt tttgacttat
    41041 tgctttattc cgctctggat gtgcaataat cggtacaaag cctttactct gtaattcgaa
    41101 aaataattga tcagtatagt gtggaacttc atttgatgga aattctatta gtaaatagcg
    41161 tgaatcatta ataccgttaa taacttttcg atcaatatca ttaaggattt gatcggtaat
    41221 tcttatttcc tgaccataat aaaactttag atttagtgct tgtacttcct ctaagctttc
    41281 aatatggttt aaacatgatt tcactttttc tataggtgtg gtatatcgag gatgtaagtg
    41341 atgtgatgtt acaatgattt ctgtaacacc ttgtgttgtc gcttgtttta aaagatccat
    41401 catctctgtt tcatttgtcg gaccgtcatc gatattaggc aatatatggt tatgaatatc
    41461 aatcatactt attcatctcc ataatagtga taataactag aagatttatc gacctttgtc
    41521 ttgttcaaaa tgacacctag aatgttactg cctgcttttt ccataagtgc ttttgctttt
    41581 ttaacttcat ttttatcatt tttttcacta tcaattacta acagactatc tttaatagca
    41641 cgcgcatata gttgtgcatc agtcacagta ttaactggcg gtgtatcgac aataataatg
    41701 tcgtaacgtt tattaaacag atcaactaat tctttgaacc tttcagaccc aattaactca
    41761 gatggatttg gaggtacagg gccagctgtt agcaaatcta aattttcaat ttctgtcgac
    41821 gtaattgctt ctgacatagt cgttcgacca atgattaagc ttgatagtcc attattattt
    41881 tgctcattaa aaatatagtt ttgtgttggc ttacgcatat cgccatcaat aactaatgtc
    41941 ttatagcctg cttgtgcata agtaatcgct acattcgata caactgtact tttacctgca
    42001 ccaggctttt cagaagtaac caataagcgc tttacttcac catttgcttt tgaaaacatg
    42061 atgtttgaac gtatacctcg aaacttttca ctaattgttg attttggttt ttcatataca
    42121 aatagtgttg ttgtcgtatt ttcctttttt gacataagta gcaaccatcc ttaattaaat
    42181 ttttgaattg aacccaatac aggcaatcct aattctttct ctacatcttc ttcatcttta
    42241 attcgcttat cgaaaattac tttgaagaag atatatataa gcgcgacaac taatcctaaa
    42301 aagaatgcac cgattagatt cactacagtt tttggtgcga ctttaactgc tgtaccgtct
    42361 gctttagata aaattgatac gttatccaca ctcataatct tcggaatttg tttacttgta
    42421 actttagcga agctattcgc aattttttcc gaatcttgtt tatgaccact tttaacttgg
    42481 atgttgataa gttgcgtatt ttcttggttt gtaattgtca acatactcga caatttagat
    42541 ggtgaatact tatcatttaa gtcctttgac acctcatcta aaattctagg acttttaaca
    42601 atttctttat acgtatttac aagttgaata ttactttgaa cctcttgcgc cataaactga
    42661 ggattgtcac ccttagtttg attcactaaa atttgagtat tagcttgata tttaggtgat
    42721 aagacgaaaa atgtaacaat agcgctaata attaaaaata atagcggtaa aataattaaa
    42781 atcttcaagt ttttttgtaa tacttcttta atttttgtta attcta
//
LOCUS       DAXWFK010000004        41882 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.004, whole genome shotgun sequence.
ACCESSION   DAXWFK010000004 DAXWFK010000000
VERSION     DAXWFK010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 41882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41882
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.004"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            37..1155
                     /gene="ald"
                     /locus_tag="ACGEXS_000138"
     CDS             37..1155
                     /gene="ald"
                     /locus_tag="ACGEXS_000138"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0042853 - L-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HFF8011190.1"
                     /translation="MLVAVVKELKQGEGRVACTPENVRKLTDAGHKVIVEKNAGIGSG
                     FSNDMYEKEGAKIVTHEQAWEADLVIKVKEPHESEYQYFKKNQIIWGFLHLASSKEIV
                     EKMQEVGVTAISGETIIKNGKAELLAPMSAIAGQRSAIMGAYYSEAQHGGQGTLVTGV
                     HENVDIPGSTYVIFGGGVAATNAANVALGLNAKVIIIELNDDRIKYLEDMYAEKDVTV
                     VKSTPENLAEQIKKADVFISTILIPGAKPPKLVTREMVKSMKKGSVLIDIAIDQGGTI
                     ETIRPTTISDPVYEEEGVIHYGVPNQPGAVPRTSTMALAQGNIDYILEICDKGLEQAI
                     KDNEALSTGVNIYQGQVTNQGLASSHDLDYKEILNVIE"
     gene            1250..2290
                     /gene="tdcB"
                     /locus_tag="ACGEXS_000139"
     CDS             1250..2290
                     /gene="tdcB"
                     /locus_tag="ACGEXS_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFF8011191.1"
                     /translation="MTTNTVTLQTAHIVSLGDIEEAKASIKPFIRRTPLIKSMYLSQS
                     ITKGNVFLKLENMQFTGSFKFRGASNKINHLTDEQKEKGIIAASAGNHAQGVALTAKL
                     LGIDATIVMPETAPQAKQQATKGYGAKVILKGKNFNETRLYMEELAKENGMTIVHPYD
                     DKFVMAGQGTIGLEILDDIWNVNTVIVPVGGGGLIAGIATALKSFNPSIHIIGVQSEN
                     VHGMAESFYKRDLTEHRVDSTIADGCDVKVPGEQTYEVVKHLVDEFILVTEEEIEHAM
                     KDLMQRAKIITEGAGALPTAAILSGKINNKWLEDKNVVALVSGGNVDLTRVSGVIEHG
                     LNIADTSKGVVG"
     gene            2321..3643
                     /locus_tag="ACGEXS_000140"
     CDS             2321..3643
                     /locus_tag="ACGEXS_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFF8011192.1"
                     /translation="MSNGKELQKNIGFFSAFAIVMGTVIGSGVFFKISNVTEVTGTAG
                     MALFVWFLGGIITICAGLTAAELAAAIPETGGLTKYIEYTYGDFWGFLSGWAQSFIYF
                     PANVAALSIVFATQLINLFHLSIGSLIPIAIASALSIVLINFLGSKAGGILQSVTLVI
                     KLIPIIVIVIFGIFQSGDITFSLIPTTGNSGNGFFTAIGSGLLATMFAYDGWIHVGNV
                     AGELKNPKRDLPLAISVGIGCIMAVYLLINATFLLTLPIELLAGNLNAASDTSKILFG
                     ENGGKIITIGILISVYGTINGYTMTGMRVPYAMAERKLLPFSHLFAKLTKSGAPWFGA
                     IIQLIIAIIMMSMGAFDTITNMLIFVIWLFYCMSFVAVIILRKREPNMERPYKVPLYP
                     IIPLIAILAGSFVLINTLFTQFILAIIGILITALGIPVYYYKKKQKAA"
     gene            3800..5191
                     /gene="norB"
                     /locus_tag="ACGEXS_000141"
     CDS             3800..5191
                     /gene="norB"
                     /locus_tag="ACGEXS_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorB"
                     /protein_id="HFF8011193.1"
                     /translation="MEKPSREAFEGNNKLLIGIVLSVITFWLFAQSLVNVVPILEDSF
                     NTDIGTVNIAVSITALFSGMFVVGAGGLADKYGRIKLTNIGIILNILGSLLIIISNIP
                     LLLIIGRLIQGLSAACIMPATLSIIKSYYIGKDRQRALSYWSIGSWGGSGVCSFFGGA
                     VATLLGWRWIFILSIIISLIALFLIKGTPETKSKSISLNKFDIKGLVLLVIMLLSLNI
                     LITKGSELGVTSLLFITLLAIAIGSFSLFIVLEKRATNPLIDFKLFKNKAYTGATASN
                     FLLNGVAGTLIVANTFVQRGLGYSSLQAGSLSITYLVMVLIMIRVGEKLLQTLGCKKP
                     MLIGTGVLIVGECLISLTFLPEIFYVICCIIGYLFFGLGLGIYATPSTDTAIANAPLE
                     KVGVAAGIYKMASALGGAFGVALSGAVYAIVSNMTNIYTGAMIALWLNAGMGILSFVI
                     ILLLVPKQNDTQL"
     gene            5589..37242
                     /gene="ebh"
                     /locus_tag="ACGEXS_000142"
                     /pseudo
     CDS             5589..37242
                     /gene="ebh"
                     /locus_tag="ACGEXS_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001635093.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hyperosmolarity resistance protein Ebh"
     gene            complement(37301..37702)
                     /locus_tag="ACGEXS_000143"
     CDS             complement(37301..37702)
                     /locus_tag="ACGEXS_000143"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFF8011194.1"
                     /translation="MAKINFDAATKGNPGISTCAIVIKEDEQHYTYTHELGEMDNHTA
                     EWAACIYALEHARELNVQNALLYTDSKLIADSIEAGYVKNANFKPYFDQIEIFEKDFD
                     LLFVKWIPREQNKEANQHAQQALYKLIKKNK"
     gene            38047..38178
                     /locus_tag="ACGEXS_000144"
     CDS             38047..38178
                     /locus_tag="ACGEXS_000144"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011195.1"
                     /translation="MGPNTEDDEKSAYNNVQVWDGPQQREIGFPISTDNASWGGTTK"
     gene            complement(38243..38947)
                     /locus_tag="ACGEXS_000145"
     CDS             complement(38243..38947)
                     /locus_tag="ACGEXS_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HFF8011196.1"
                     /translation="MYNEILGLVTFIATFVLMVLMYRFFGKQGLIAWVAIGTIIANIQ
                     VIKTVEIFGISATLGNVMFASIYLATDILNDIYGRRVAKRAVWLGFSSTLIMIIVMQL
                     SLHFIPAPEDMAQKALHAIFDVVPRIALGSIVAYIIGQHIDVFIFSLIKKVFSSDKTF
                     FIRAYGSTFLSSIIDTALFVAIAFIGSLPGTVVFEIFITTYVLKLASTVFNVPFGYIA
                     KSFYRKGKIDKLDQGY"
     gene            39192..39386
                     /locus_tag="ACGEXS_000146"
     CDS             39192..39386
                     /locus_tag="ACGEXS_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HFF8011197.1"
                     /translation="MSRILTFSEQNALAKIDDLMNTYCNQCPIKTRLRKLEGKTKAHH
                     FCINECSIGKEIKQLGNELQ"
     gene            39398..39649
                     /locus_tag="ACGEXS_000147"
     CDS             39398..39649
                     /locus_tag="ACGEXS_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU N-terminal domain-containing protein"
                     /protein_id="HFF8011198.1"
                     /translation="MKIISISETPNHNTMKITLSESREGMTSDTYTKVDDSQPAFIND
                     ILKVEGVKSIFHVMDFISVDKENDANWETVLPKVEAVFE"
     gene            39687..40811
                     /locus_tag="ACGEXS_000148"
     CDS             39687..40811
                     /locus_tag="ACGEXS_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HFF8011199.1"
                     /translation="MEILRIEPTPSPNTMKVVLSYTREDKLSNTYKKVEETQPRFINQ
                     LLSIDGITSIFHVMNFLAVDKAPKADWEVILPDIKAAFSDANKVLESVNEPQIDNHFG
                     EIKAELLTFKGIPYQIKLTSADQELREQLPQTYVDHMTQAQTAHDNIVFMRKWLDLGN
                     RYGNIQEVMDGVLEEVLATYPESQLPVLVKHALEENHATNNYHFYRHVSLDEYHATDN
                     WKTRLRMLNHFPKPTFEDIPLLDLALSDEKVPVRRQAIVLLGMIESKEILPYLYKGLR
                     DKSPAVRRTAGDCISDLGYPEALPEMVLLLDDPQKIVRWRAAMFIFDEGNAEQLPALK
                     AHINDNAFEVKLQIEMAISRIENGDEALGSVWKQMANRTI"
     gene            40827..41264
                     /gene="brxA"
                     /locus_tag="ACGEXS_000149"
     CDS             40827..41264
                     /gene="brxA"
                     /locus_tag="ACGEXS_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacilliredoxin BrxA"
                     /protein_id="HFF8011200.1"
                     /translation="MNAYDAYMKEIAQQMRGELTQNGFTSLETSEAVSEYMNQVNADD
                     TTFVVINSTCGCAAGLARPAAVAVATQNEHRPTNTVTVFAGQDKEATATMREFIQQAP
                     SSPSYALFKGQDLVYFMPREFIEGRDINDIAMDLKDAFDENCK"
     gene            41688..>41882
                     /locus_tag="ACGEXS_000150"
     CDS             41688..>41882
                     /locus_tag="ACGEXS_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFF8011201.1"
                     /translation="MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLS
                     KGFPLLTTKKVSFKLVATELL"
BASE COUNT        16520 a         7230 c         7554 g        10578 t
ORIGIN      
        1 gtgaaattat tcacaaatga aagaggagtg ttgcaaatgt tagttgcagt agtcaaagaa
       61 ttaaaacaag gtgaaggacg tgtagcttgc acacccgaaa atgtgcgtaa gttaacggat
      121 gcaggacata aagtaattgt tgaaaaaaat gctggcattg gttcaggatt ttctaacgat
      181 atgtatgaaa aagaaggcgc taagatcgta actcacgaac aagcatggga agctgatctt
      241 gttatcaaag taaaagaacc tcatgaaagc gaatatcaat atttcaaaaa gaatcaaatt
      301 atctggggat ttttacatct agcatcttca aaagaaatag tagaaaaaat gcaagaagtt
      361 ggtgtaactg cgattagtgg tgaaaccatt ataaaaaatg gaaaagcaga attattagcg
      421 ccaatgagtg ctatagcagg tcaacgctca gcaattatgg gagcttacta ctctgaagca
      481 caacatggtg gtcaaggtac tttagtgact ggtgtacatg aaaatgtgga tatacctggt
      541 agtacatatg tgattttcgg tggtggagta gcagcaacaa atgcagcaaa tgttgccttg
      601 ggactaaatg ctaaagtaat cattatcgag ttaaacgatg accgcattaa atatcttgaa
      661 gatatgtatg cagaaaaaga tgtcacagta gtcaaatcaa caccagaaaa tttagcagaa
      721 caaattaaga aagcagatgt atttatttct acaattttaa ttccaggtgc gaaaccgcca
      781 aaattggtta ctcgtgagat ggttaaatca atgaaaaaag gttcagtatt aatcgatata
      841 gctattgacc aaggtggaac tattgaaaca attagaccaa ctacaatttc tgatccagtg
      901 tatgaagaag aaggtgtgat tcattatggt gtaccaaatc aaccaggagc agtcccaaga
      961 acttcaacaa tggcattagc acaaggaaat attgattata tattagaaat ttgtgacaaa
     1021 ggcttagaac aagcaattaa agataatgaa gccttaagta ctggtgtaaa catttaccaa
     1081 ggacaagtga caaatcaagg attagcttca tcacatgacc tagattataa agaaatatta
     1141 aatgttatcg aatagatagt aatttaaatg aaattgagtg aaatgaatat tttaaatata
     1201 gcattatagt ttggactaaa aatttacaaa acggaaggat gtaattatta tgacaaccaa
     1261 cacagttaca ttacaaacag cacatattgt aagtttagga gatatcgaag aagctaaagc
     1321 aagcattaaa ccatttattc gtcgaacacc tctaattaaa tcaatgtatt taagccaaag
     1381 tataactaaa gggaatgtat ttctaaaatt agaaaatatg caattcacag gatcttttaa
     1441 atttagaggc gctagcaata aaattaatca cttaacagat gaacaaaaag aaaaaggcat
     1501 tatcgcagca tctgctggga accatgcaca aggtgttgct ttaacagcta aattattagg
     1561 cattgatgca acgattgtaa tgcctgaaac agcaccacaa gcgaaacaac aagcaacaaa
     1621 aggctatggg gcaaaggtta ttttaaaagg taaaaacttt aacgaaacta gactttatat
     1681 ggaagaatta gcgaaagaaa atggcatgac aatcgttcat ccatatgacg ataagtttgt
     1741 aatggcaggc caaggaacaa ttggtttaga aattttagat gatatttgga atgtgaatac
     1801 agtcatcgta ccagttggcg gtggaggatt aattgcaggt attgccaccg cattaaaatc
     1861 atttaaccct tcaattcata ttatcggtgt tcaatctgag aatgttcatg gtatggctga
     1921 gtctttctat aagagagatt taactgaaca tcgagtggat agcacaatag cagatggttg
     1981 tgatgtaaaa gttcctggtg aacaaacata tgaagtagtt aaacatttag tagatgaatt
     2041 tattcttgtt actgaagaag aaattgaaca tgctatgaaa gatttaatgc agcgtgccaa
     2101 aattattact gaaggtgcag gcgcattacc aacagctgca attttaagtg gaaaaataaa
     2161 caataaatgg cttgaagata aaaatgttgt tgcattagtt tcaggcggga atgttgactt
     2221 aactagagtt tcaggtgtca ttgaacatgg actgaatatt gcagatacaa gcaagggtgt
     2281 ggtaggttaa aacatttaat cttaaaaatg aggtgtaatt atgtcaaatg gtaaagaatt
     2341 acaaaaaaat ataggtttct tctcagcgtt tgctattgtt atggggacag ttattggttc
     2401 aggagtattc tttaaaatat caaacgtaac agaagtaaca ggaacagcag gaatggcctt
     2461 gtttgtatgg ttcctaggcg gcatcattac catttgtgcg gggttaacag cagcagaact
     2521 tgctgctgca atccctgaaa caggtggctt aacgaagtat atagaatata catacggtga
     2581 tttctggggc ttcctatcag gttgggcgca atcatttatt tattttccag ctaacgtagc
     2641 agcattgtct atcgtatttg cgacacagct aattaattta ttccatttat ctataggttc
     2701 gttaatacca atagcaatcg catctgcgtt atctattgtg ttgataaatt tcctaggttc
     2761 aaaagcaggc ggaattttac aatcagttac tttagtaatt aaactgattc caatcatcgt
     2821 tattgtaatt tttggtattt ttcaatctgg agatatcact ttttcattaa ttccaactac
     2881 aggtaattca ggaaatggct tctttacagc aattggtagt ggtttattag caactatgtt
     2941 tgcatatgat ggttggattc atgtaggaaa tgttgcgggg gaacttaaaa atcctaaacg
     3001 cgatttacct ttagcgattt cagttggtat cggttgtatt atggctgtgt atttattaat
     3061 taacgctaca ttcttattaa cgttgccaat agaactactt gctggtaatt taaatgcagc
     3121 ttcagataca tcaaaaatat tatttggtga aaatggcggt aagattatta caatcggtat
     3181 attaatttct gtttatggta cgatcaatgg ctatactatg actggtatgc gcgtaccata
     3241 tgcaatggct gaaagaaaat tattgccatt tagccattta ttcgcaaaat taacaaaatc
     3301 tggcgcacca tggtttggcg caattataca acttataatc gctatcatca tgatgtcaat
     3361 gggagcattt gatacaatta caaatatgtt aatctttgtt atttggttgt tctattgtat
     3421 gtcatttgtt gcggtaataa ttttaagaaa acgtgaacca aatatggaac gaccatataa
     3481 agtaccgtta tatccgatca tacctttaat tgctattttg gcaggatcat ttgtattaat
     3541 taatacactg tttacacaat ttatattagc aatcattgga attctaataa cagcacttgg
     3601 tataccagtt tattactata aaaagaaaca aaaagcagca taaggtaaga taactagcat
     3661 tgagaataaa tggatggact actaataaat ttaaagtttt acacattaaa atcaaaaacc
     3721 attcaattat tctatggaac agacaaattt ctgttatgga atttgtctgt ttttcaaaag
     3781 tatagggagg caaatagaga tggaaaagcc gtcaagagag gcatttgaag gcaataataa
     3841 gttgttaata ggaattgttc taagtgtaat aacgttttgg ctatttgcac aatcattggt
     3901 taatgttgta ccaatacttg aagatagttt caatacagat attggaacgg ttaatatcgc
     3961 cgttagtata actgctttat tttcaggaat gtttgtagta ggagcaggtg gtcttgctga
     4021 taaatatggc agaattaaac tcacgaacat tggtattatc ttaaatatat taggttcatt
     4081 attaatcatt atttcaaata ttcctttatt acttattata ggaagattaa ttcaaggact
     4141 ttcagcagca tgtattatgc ctgcaacttt gtctattatt aagtcatatt acattgggaa
     4201 agatagacaa cgcgctttaa gttattggtc aattggctca tggggcggct ctggtgtttg
     4261 ttcatttttt ggaggtgcag ttgcaacgct tttaggttgg cgttggattt tcatcctatc
     4321 aattataatt tcattaattg cactgtttct tattaaaggc acacctgaaa ctaaatctaa
     4381 atcgatttct ctaaataaat ttgacattaa aggtctggtt cttttagtca ttatgctcct
     4441 cagtttaaat attttaatta ctaaaggatc agaattaggt gtaacctcac ttctttttat
     4501 tactttatta gctattgcaa ttggatcttt tagtttattt atagttcttg aaaagcgtgc
     4561 tacaaatcct ttaatcgatt ttaaattatt taaaaataaa gcttacacag gtgcaacagc
     4621 ttcaaacttt ttgttaaatg gtgttgcagg aacattaata gtagccaaca catttgttca
     4681 aagaggttta ggatattctt cattgcaagc aggaagttta tcaatcactt atttagtaat
     4741 ggtactaatt atgattcgtg ttggtgaaaa gttacttcaa acactcggat gcaagaaacc
     4801 aatgttaatt ggaacaggag ttcttattgt cggagaatgt ctcatttcat taactttctt
     4861 gccagaaata ttctatgtca tttgttgtat tataggttat ttattctttg gtttaggact
     4921 agggatatat gctacaccat caacagatac agcaattgca aatgcaccgt tagaaaaagt
     4981 aggcgttgct gcaggtatct ataaaatggc ttctgcatta ggtggagcat ttggcgtcgc
     5041 attgagtggt gcagtatatg caatcgtatc aaatatgaca aacatttata caggtgcaat
     5101 gattgcatta tggttaaatg caggtatggg aatattatca ttcgttatca ttttgttact
     5161 tgtgcctaaa caaaacgaca ctcaattatg ataattgaga attaaattga aatcatacaa
     5221 gtcgctacaa tattaaacaa aaatataaac cgattcttat gtgtcattat tttaaatgaa
     5281 catagggatt ggttttttat tactctttta cgctacttta tttataatta ttataaattg
     5341 tcacaaattc aatttacctt acaatatatt ttgtgttatt atattctgga gcataaataa
     5401 attgttcaac acatagttgt aatgtgtttc aatacttttt ggatagattg cgaaattgta
     5461 ttgaatcgtc atcgttttaa atttttaaat gagaatggaa tgagcattac aatacacaag
     5521 caatcaaaag taaatacatt cacaacacaa cagagacata acaacaagat aaggagtgaa
     5581 caatagctgt gaattatcgt gataaaattc aaaagtttag tattcgtaaa tatacagttg
     5641 gtacattttc aactgtcatt gcgacattgg tatttttagg attcaataca tcacaagcac
     5701 atgctgctga aacaaatcaa ccagcaagcg tggttaaaca gaaacaacaa agtaataatg
     5761 aacagactga gaatcgagaa tctcaagtac aaaattctca aaattcacaa aatggtcaat
     5821 cattatctgc tactcatgaa aatgagcaac caaatattag tcaagctaat ttagtagatc
     5881 aaaaagtagc gcaatcatct actactaatg atgaacaacc agcatctcaa aatgtaaata
     5941 caaagaaaga ttcggcaacg gctgcgacaa cacaaccaga taaagaacaa agtaagcata
     6001 aacaaaacga aagtcaatct gctaataaaa atggaaacga caatagagcg gctcatgtag
     6061 aaaatcatga agcaaatgta gtaacagctt cagattcatc tgataatggt aacgtacaac
     6121 atgaccgaaa tgaattacaa gcgttttttg atgcaaatta tcatgattat cgctttattg
     6181 accgtgaaaa tgcagattct ggcacattta actatgtaaa aggcattttt gataagatta
     6241 atacgttatt aggcagtaat gatccaataa acaataaaga cttgcaactt gcatacaaag
     6301 aattggaaca agctgttgct ttaattcgta caatgcctca acgtcaacag actagccgac
     6361 gttcaaatag aattcaaacg cgttcggttg agtcaagagc tgcagagcct agatcagtat
     6421 cagactatca aaatgcaaat tcatcatatt atgttgaaaa tgctaatgat ggttcgggct
     6481 atcctgttgg tacatatatc aatgcttcta gtaaaggggc gccatataat ttaccaacta
     6541 caccatggaa tacattgaag gcctctgact caaaggaaat tgctcttatg acagcgaaac
     6601 aaactggaga cgggtaccaa tgggttatta agtttaataa aggacatgct ccacatcaaa
     6661 atatgatctt ttggtttgca ttaccagcag accaagtgcc agtaggaaga actgactttg
     6721 taacagttaa ttcagatgga acaaatgtac aatggagtca tggagcagga gcaggtgcaa
     6781 ataaaccact tcaacaaatg tgggaatatg gagtaaatga tcctcatcgt tcacatgact
     6841 ttaaaataag aaatagaagt ggccaagtaa tatatgactg gccaactgtc catatttatt
     6901 ctttagaaga tttatctaga gcgagtgatt attttagtga agctggagcg acacctgcta
     6961 ctaaagcttt tggtagacaa aattttgaat atattaatgg tcaaaaacct gctgaatcac
     7021 cgggtgttcc taaagtttat actttcatcg gtcaaggtga tgcaagttat acaatttcat
     7081 ttaaaacaca aggtccaact gttaataaat tgtactatgc agcaggtggg cgtgctttag
     7141 agtacaatca attatttatg tacagtcaac tatacgtcga atcaacgcaa gaccatcaac
     7201 aacgtcttaa tggtttaaga caagtggtta atcgtacata tcgcataggt acaactaaac
     7261 gtgtagaagt gagtcaagga aatgtacaaa cgaaaaaggt attagaaagt acaaacctaa
     7321 atatagatga ttttgttgat gatcctttaa gttatgttaa gacgccgagt aataaagtgt
     7381 taggatttta ttcgaataat gcaaatacta atgcttttag accgggtgga gcccaacaat
     7441 taaatgaata tcaattaagt caattattta ctgatcaaaa attacaagaa gcagcaagaa
     7501 ctagaaaccc aataagatta atgattggtt tcgactatcc tgatgcttat ggtaatagtg
     7561 aaactttagt tcctgttaac ttaacggtat tacctgaaat ccaacataat attaaattct
     7621 ttaaaaatga cgatactcaa aatattgctg aaaaaccatt ttcaaaacaa gctgggcatc
     7681 cagttttcta tgtatatgca ggtaaccaag ggaatgcttc cgtgaattta ggtggtagcg
     7741 taacatctat tcaaccatta cgtattaatt taacaagtaa tgagaatttt acagataaag
     7801 attggcaaat tacaggtatt ccgcgtacat tacacattga aaactcgaca aatagaccta
     7861 ataatgccag agaacgcaat attgaacttg ttggtaactt attaccaggg gattactttg
     7921 gaacgatacg ttttggacgt aaagaacaat tattcgaaat tcgtgttaaa ccacatacac
     7981 caacaattac aacgacagct gagcaattaa gaggtacagc attacaaaaa gtgcctgtta
     8041 atatttcggg aataccgttg gatccatcgg cattggttta tttagttgca ccaacaaatc
     8101 aaactacgaa tggtggtagt gaggcagatc aaataccatc tggttatacg atacttgcga
     8161 ctggtacacc tgatggggtg cataatacaa ttactatacg accgcaagat tatgttgtat
     8221 tcataccacc tgtaggtaaa caaattagag cagtagttta ttataataaa gtagttgcat
     8281 ctaatatgag taatgctgtt actattttgc cagatgacat tccaccaaca atcaataatc
     8341 ctgttggaat aaatgccaaa tactatcgag gcgacgaagt caactttaca atgggtgtct
     8401 ctgatagaca ttctggtata aaaaatacaa ctattacgac attgccgaat ggttggacat
     8461 caaatttaac aaaagcggat aagaataatg gctcattatc tattacaggt agagtgagta
     8521 tgaatcaggc atttaacagt gatattacat ttaaagtgtc agcgacagac aatgtcaata
     8581 atacgacaaa tgatagtcaa tctaaacatg tttcaattca tgtaggtaaa attagtgaag
     8641 atgctcatcc gattgtatta ggaaatactg agaaagttgt agtagtcaat ccgactgctg
     8701 tatctaatga tgaaaagcaa agcataatta ctgcctttat gaataaaaac caaaatataa
     8761 gaggatattt agcatcaact gatccagtaa ctgtcgataa taatggtaat gtcacattac
     8821 attaccgtga tggctcatcg acaacgcttg atgctacaaa tgtgatgaca tacgaaccag
     8881 ttgtgaaacc tgaataccaa actgtcaatg ctgctaaaac agcaacggta acgattgcta
     8941 aaggacaatc atttagtatt ggtgatatta aacaatattt tactttaagt aatggacaac
     9001 ctattccaag tggcacattt acaaatatta catctgatag aactattcca actgcacaag
     9061 aagttagtca aatgaacgca ggcacgcagt tataccatat aactgctaca aatgcgtatc
     9121 ataaagatag tgaagacttc tatattagtt tgaaaatcat cgatgtgaaa caaccagaag
     9181 gcgatcaacg tgtatatcgt acatcaacat atgatttaac tactgatgaa atctcaaaag
     9241 taaaacaagc atttattaat gcaaatagag atgtaattac gcttgccgaa ggtgatattt
     9301 cagttacaaa tacacctaat ggtgctaatg taagtactat tacagtaaat attaataaag
     9361 gtcgattaac gaaatcattc gcgtcaaacc tagctaatat gaatttcttg cgttgggtta
     9421 atttcccaca agattataca gtgacatgga cgaatgcaaa aattgcaaac agaccaacag
     9481 atggtggttt atcatggtct gatgaccata aatctttaat ttatcgttat gatgctacat
     9541 taggtactca aattacgacg aatgatattt taacaatgtt aaaagcaaca actacagtgc
     9601 ctggattgcg aaataacatt actggtaatg aaaaatcaca agcagaagct ggcggaagac
     9661 ctaactttag aacgactggt tattcacaat caaatgcgac aactgatggt caacgtcaat
     9721 ttacgttgaa tggtcaagtg attcaagtgt tagacatcat caacccttca aacggttatg
     9781 gtgggcaacc tgttacaaat tcaaatactc gtgcaaacca tagtaactca actgttgtta
     9841 acgtaaacga accggcagct aatggtgctg gcgcatttac aattgaccac gttgtaaaaa
     9901 gtaattctac acataatgca agtgatgcag tttataaagc acagttatac ttaacgccat
     9961 atggtccaaa acaatatgtt gaacatttaa atcaaaatac aggaaatact actgacgcta
    10021 ttaacattta ttttgtacca agtgacttag tgaatccaac aatttcagta ggtaattaca
    10081 ctaatcatca agtgttctca ggtgaaacat ttacaaatac tattacagcg aatgataact
    10141 ttggtgtgca atctgtaact gtaccaaata catcacaaat tacaggtact gttgataata
    10201 accatcaaca tgtttctgca acggcaccaa atgtgacatc agcaactaat aagacaatca
    10261 atttattagc aactgataca agcggcaata cagctacaac ttcgttcaat gtaacagtga
    10321 aacctttgcg tgataaatat cgagttggta cttcatcaac ggctgctaat cctgtgagaa
    10381 ttgccaatat ttcgaataat gcgacagtat cacaagctga tcaaacgaca attattaatt
    10441 cgttaacgtt tactgaaaca gtaccaaata gaagttatgc aagagcaagt gcgaatgaaa
    10501 tcactagtaa aacagttagt aatgtcagtc gtactggaaa taatgccaat gtcacagtaa
    10561 ctgttactta tcaagatgga acaacatcaa cagtgactgt acctgtaaag catgtcattc
    10621 cagaaatcgt tgcacattcg cattacactg tacaaggcca agacttccca gcaggtaatg
    10681 gttctagtgc atcagattac tttaagttat ctaatggtag tgacattgca gatgcaacta
    10741 ttacatgggt aagtggacaa gcgccaaata aagataatac acgtattggt gaagatataa
    10801 ctgtaactgc acatatctta attgatggcg aaacaacgcc gattacgaaa acagcaacat
    10861 ataaagtagt aagaactgta ccgaaacatg tctttgaaac agccagaggt gttttatacc
    10921 caggtgtttc agatatgtat gatgcgaaac aatatgttaa gccagtaaat aattcttggt
    10981 cgacaaatgc gcaacatatg aatttccaat ttgttggaac atatggtcct aacaaagatg
    11041 ttgtaggcat atctactcgt cttattagag tgacatatga taatagacaa acagaagatt
    11101 taactatttt atctaaagtt aaacctgacc cacctagaat tgacgcaaac tctgtgacat
    11161 ataaagcagg tcttacaaac caagaaatta aagttaataa cgtattaaat aactcgtcag
    11221 taaaattatt taaagcagat aatacaccat taaatgtcac aaatattact catggtagcg
    11281 gttttagttc ggttgtgaca gtaagtgacg cgttaccaaa tggcggaatt aaagcaaaat
    11341 cttcaatttc aatgaacaat gtgacgtata cgacgcaaga cgaacatggt caagttgtta
    11401 cagtaacaag aaatgaatct gttgattcaa atgacagtgc aacagtaaca gtgacaccac
    11461 aattacaagc aactactgaa ggcgctgtat ttattaaagg tggcgacggt tttgatttcg
    11521 gacacgtaga aagatttatt caaaacccgc cacatggggc aacggttgca tggcatgata
    11581 gtccagatac atggaagaat acagtcggta acactcataa aactgcggtt gtaacattac
    11641 ctaatggtca aggtacgcgt aatgttgaag ttccagtcaa agtttatcca gttgctaatg
    11701 caaaggcgcc atcacgtgat gtgaaaggtc aaaatttgac taatggaacg gatgcgatga
    11761 actacattac atttgatcca aatacaaaca caaatggtat cactgcagca tgggcaaata
    11821 gacaacaacc aaataaccaa caagcaggcg tgcaacattt aaatgtcgat gtcacatatc
    11881 caggtatttc agctgctaaa cgagttcctg ttactgttaa tgtatatcaa tttgaattcc
    11941 ctcaaactac ttatacgaca acggttggag gcactttagc aagtggtacg caagcatcag
    12001 gatatgcaca tatgcaaaat gctactggtt taccaacaga tggatttacg tataaatgga
    12061 atcgtgatac tacaggtaca aatgacgcaa actggtcagc tatgaataaa ccgaatgtgg
    12121 ctaaagtcgt taacgcaaaa tatgacgtca tctataacgg acatactttt gcaacatctt
    12181 taccagcgaa atttgtagta aaagatgtgc aaccagcgaa accaactgtg actgaaacag
    12241 cggcaggagc gattacaatt gcacctggag caaaccaaac agtgaataca catgccggta
    12301 acgtaacgac atacgctgat aaattagtta ttaaacgtaa tggtaacgtt gtgacgacat
    12361 ttacacgtcg caataatacg agtccatggg tgaaagaagc atctgcagca actgtagcag
    12421 gtattgctgg aactaataat ggtattactg ttgcagcagg tactttcaac cctgctgata
    12481 caattcaagt tgttgcaacg caaggaagcg gagagacagt gagtgatgag caacgtagtg
    12541 atgatttcac agttgtcgca ccacaaccga accaagtgac tactaagatt tggcaaaatg
    12601 gtcatattga tatcacgcct aataatccat caggacattt aattaatcca actcaagcaa
    12661 tggatattgc ttacactgaa aaagtgggta atggtgcaga acatagtaag acaattaatg
    12721 ttgttcgtgg tcaaaataat caatggacaa ttgcgaataa gcctgactat gtaacgttag
    12781 atgcacaaac tggtaaagtg acgttcaatg ccaatactat aaaaccaaat tcatcaatca
    12841 caattactcc gaaagcaggt acaggtcact cagtaagtag taatccaagt acattaactg
    12901 caccggcagc tcatactgtc aacacaactg aaattgtgaa agattatggt tcaaatgtaa
    12961 cagcagctga aattaacaat gcagttcaag ttgctaataa acgtactgca acgattaaaa
    13021 atggcacagc aatgcctact aatttagctg gtggtagcac aacgacgatt cctgtgacag
    13081 taacttacaa tgatggtagt actgaagaag tacaagagtc cattttcaca aaagcggata
    13141 aacgtgagtt aatcacagct aaaaatcatt tagatgatcc agtaagcact gaaggtaaaa
    13201 agccaggtac aattacgcag tacaataatg caatgcataa tgcgcaacaa caaatcaata
    13261 ctgcgaaaac agaagcacaa caagtgatta ataatgagcg tgcaacacca caacaagttt
    13321 ctgacgcact aactaaagtt cgtgcagcac aaactaagat tgatcaagct aaagcattac
    13381 ttcaaaataa agaagataat agccaattag taacgtctaa aaataactta caaagttctg
    13441 tgaaccaagt accatcaact gctggtatga cgcaacaaag tattgataac tataatgcga
    13501 agaagcgtga agcagaaact gaaataactg cagctcaacg tgttattgac aatggcgatg
    13561 caactgcaca acaaatttca gatgaaaaac atcgtgtcga taacgcatta acagcattaa
    13621 accaagcgaa acatgattta actgcagata cacatgcctt agagcaagca gtgcaacaat
    13681 tgaatcgcac aggtacaacg actggtaaga agccggcaag tattactgct tacaataatt
    13741 cgattcgtgc acttcaaagt gacttaacaa gtgctaaaaa tagcgctaat gctattattc
    13801 aaaagccaat aagaacagta caagaagtgc aatctgcgtt aacaaatgta aatcgtgtca
    13861 atgagcgatt aacgcaagca attaatcaat tagtaccttt agctgataat agtgctttaa
    13921 aaactgctaa gacgaaactt gatgaagaaa tcaataaatc agtaactact gatggtatga
    13981 cacaatcatc aatccaagca tatgaaaatg ctaaacgtgc gggtcaaaca gaatcaacaa
    14041 atgcacaaaa tgttattaac aatggtgatg cgactgacca acaaattgcc gcagaaaaaa
    14101 caaaagtaga agaaaaatat aatagcttaa aacaagcaat tgctggatta actccagact
    14161 tggcaccatt acaaactgca aaaactcagt tgcaaaatga tattgatcag ccaacgagta
    14221 cgactggtat gacaagcgca tctattgcag catttaatga aaaactttca gcagctagaa
    14281 ctaaaattca agaaattgat cgtgtattag cctcacatcc agatgttgcg acaatacgtc
    14341 aaaacgtgac agcagcgaat gccgctaaat cagcacttga tcaagcacgt aatggcttaa
    14401 cagtcgataa agcgccttta gaaaatgcga aaaatcaact acaacatagt attgacacgc
    14461 aaacaagtac aactggtatg acacaagact ctataaatgc atacaatgcg aagttaacag
    14521 ctgcacgtaa taagattcaa caaatcaatc aagtattagc aggttcaccg actgtagaac
    14581 aaattaatac aaatacgtct acagcaaatc aagctaaatc tgatttagat catgcacgtc
    14641 aagctttaac accagataaa gcgccgcttc aaactgcgaa aacgcaatta gaacaaagca
    14701 ttaatcaacc aacggataca acaggtatga cgaccgcttc gttaaatgcg tacaaccaaa
    14761 aattacaagc agcgcgtcaa aagttaactg aaattaatca agtgttgaat ggcaacccaa
    14821 ctgtccaaaa tatcaatgat aaagtgacag aggcaaacca agctaaggat caattaaata
    14881 cagcacgtca aggtttaaca ttagatagac agccagcgtt aacaacatta catggtgcat
    14941 ctaacttaaa ccaagcacaa caaaataatt tcacgcaaca aattaatgct gctcaaaatc
    15001 atgctgcgct tgaaacaatt aagtctaaca ttacggcttt aaatactgcg atgacgaaat
    15061 taaaagacag tgttgcggat aataatacaa ttaaatcaga tcaaaattac actgacgcaa
    15121 caccagctaa taaacaagcg tatgataatg cagttaatgc ggctaaaggt gtcattggag
    15181 aaacgactaa tccaacgatg gatgttaaca cagtgaacca aaaagcagca tctgttaaat
    15241 cgacgaaaga tgctttagat ggtcaacaaa acttacaacg tgcgaaaaca gaagcaacaa
    15301 atgcgattac gcatgcaagt gatttaaacc aagcacaaaa gaatgcatta acacaacaag
    15361 tgaatagtgc acaaaacgtg caagcagtaa atgatattaa acaaacgact caaagcttaa
    15421 atactgctat gacaggttta aaacgtggcg ttgctaatca taaccaagtc gtacaaagtg
    15481 ataattatgt caacgcagat actaataaga aaaatgatta caacaatgca tacaaccatg
    15541 cgaatgacat tattaatggt aatgcacaac atccagttat aacaccaagt gatgttaaca
    15601 atgctttatc aaatgtcaca agtaaagaac atgcattgaa tggtgaagct aagttaaatg
    15661 ctgcgaaaca agaagcgaat actgcattag gtcatttaaa caatttaaat aatgcacaac
    15721 gtcaaaactt acaatcgcaa attaatggtg cgcatcaaat tgatgcagtt aatacaatta
    15781 agcaaaatgc aacaaacttg aatagtgcaa tgggtaactt aagacaagct gttgcagata
    15841 aagatcaagt gaaacgtaca gaagattatg cggatgcaga tacagctaaa caaaatgcat
    15901 ataacagtgc agtttcaagt gccgaaacaa tcattaatca aacaacaaat ccaacgatgt
    15961 ctgttgatga tgttaatcgt gcaacttcag ctgttacttc taataaaaat gcattaaatg
    16021 gttatgaaaa attagcacaa tctaaaacag atgctgcaag agcaattgat gcattaccac
    16081 atttaaataa tgcacaaaaa gcagatgtta aatctaaaat taatgctgca tcaaatattg
    16141 ctggcgtaaa tactgttaaa caacaaggta cagatttaaa tacagcgatg ggtaacttgc
    16201 aaggtgcaat caatgatgaa caaacgacgc ttaatagtca aaactatcaa gatgcgacac
    16261 ctagtaagaa aacagcatac acaaatgcgg tacaagctgc gaaagatatt ttaaataaat
    16321 caaatggtca aaataaaacg aaagatcaag ttactgaagc gatgaatcaa gtgaattctg
    16381 ctaaaaataa cttagatggt acgcgtttat tagatcaagc gaagcaaaca gcaaaacagc
    16441 agttaaataa tataacgcat ttaacaactg cacaaaaaac gaatttaaca aaccaaatta
    16501 atagtggtac tactgtcgct ggtgttcaaa cggttcaatc aaatgccaat acattagatc
    16561 aagccatgaa tacgttaaga caaagtattg ccaacaaaga tgcgactaaa gcaagtgaag
    16621 attacgtaga tgctaataat gataagcaaa cagcatataa caacgcagta gctgctgctg
    16681 aaacgattat taatgctaat agtaatccag aaatgaatcc aagtacgatt acacaaaaag
    16741 cagagcaagt gaatagttct aaaacggcac ttaacggtga tgaaaactta gctgctgcaa
    16801 aacaaaatgc gaaaacgtac ttaaacacat tgacaagtat tacagatgct caaaagaaca
    16861 atttgattag tcaaattact agtgcgacaa gagtgagtgg tgttgatact gtaaaacaaa
    16921 atgcgcaaca tctagaccaa gctatggcta gcttacagaa tggtattaac aacgaatctc
    16981 aagtgaaatc atctgagaaa tatcgtgatg ctgatacaaa taaacaacaa gagtatgata
    17041 atgctattac tgcagcgaaa gcgattttaa ataaatcgac aggtccaaac actgcgcaaa
    17101 atgcagttga agcagcatta caacgtgtta ataatgcgaa agatgcattg aatggtgatg
    17161 caaaattaat tgcagctcaa aacgcagcga aacaacattt aggtacttta acgcatatca
    17221 ctacagctca acgtaatgat ttaacaaatc aaatttcaca agctacaaac ttagctggtg
    17281 ttgaatctgt taaacaaaat gcgaatagtt tagatggtgc tatgggtaac ttacaaacgg
    17341 ctatcaacga taagtcagga acattagcga gccaaaactt cttggatgct gatgagcaaa
    17401 aacgtaatgc atacaatcaa gctgtatcag cagccgaaac cattttaaat aaacaaactg
    17461 gaccgaatac agcgaaaaca gcagtcgaac aagcacttaa taatgttaat aatgcgaaac
    17521 atgcattaaa tggtacgcaa aacttaaaca atgcgaaaca agcagcgatt acagcaatca
    17581 atggcgcatc tgatttaaat caaaaacaaa aagatgcatt aaaagcacaa gctaatggtg
    17641 ctcaacgcgt atctaatgca caagatgtac agcacaatgc gactgaactg aacacggcaa
    17701 tgggcacatt aaaacatgcc atcgcagata agacgaatac gttagcaagc agtaaatatg
    17761 ttaatgccga tagcactaaa caaaatgctt acacaactaa agttaccaat gctgaacata
    17821 ttattagcgg tacgccaacg gttgttacga caccttcaga agtaacagct gcagctaatc
    17881 aagtaaacag cgcgaaacaa gaattaaatg gtgacgaaag attacgtgaa gcaaaacaaa
    17941 acgccaatac tgctattgat gcattaacac aattaaatac acctcaaaaa gctaaattaa
    18001 aagaacaagt gggacaagcc aatagattag aagacgtaca aactgttcaa acaaatggac
    18061 aagcattgaa caatgcaatg aaaggcttaa gagatagtat tgctaacgaa acaacagtca
    18121 aaacaagtca aaactataca gacgcaagtc cgaataacca atcaacatat aatagcgctg
    18181 tgtcaaatgc gaaaggtatc attaatcaaa ctaacaatcc gactatggat actagtgcga
    18241 ttacccaagc tacaacacaa gtgaataatg ctaaaaatgg tttaaacggt gctgaaaact
    18301 taagaaatgc acaaaacact gctaagcaaa acttaaatac attatcacac ttaacaaata
    18361 accaaaaatc tgccatctca tcacaaattg atcgtgcagg tcatgtgagt gaggtaactg
    18421 ctactaaaaa tgcagcaact gagttgaata cgcaaatggg taacttggaa caagctatcc
    18481 atgatcaaaa cacagttaaa caaagtgtta aatttactga tgcagataaa gctaaacgtg
    18541 atgcgtatac aaatgcggta agcagagctg aagcaattct gaataaaacg caaggtgcaa
    18601 atacgtctaa acaagatgtt gaagcggcta ttcaaaatgt ttcaagtgct aaaaatgcat
    18661 tgaatggtga tcaaaacgtt acaaatgcga agaatgcagc taaaaatgca ttaaataact
    18721 taacgtcaat taataatgca caaaaacgtg acttaacaac taaaattgat caagcaacaa
    18781 ctgtagctgg tgttgaagct gtatctaata cgagtacaca attgaataca gcgatggcta
    18841 acttgcaaaa tggtattaat gataaaacaa atacactagc aagtgaaaac tatcatgatg
    18901 ctgattcaga taagaaaact gcttatactc aagccgttac gaacgcagaa aatattttaa
    18961 ataaaaatag tggatcaaat ttagacaaaa ctgccgttga aaacgcgttg tcacaagttg
    19021 ctaatgcgaa aggtgcccta aatggtaacc ataatttaga gcaagctaaa tcaaatgcaa
    19081 acactactat aaacggactt caacatttaa caactgctca aaaagataaa ttgaaacaac
    19141 aagtgcaaca agcacaaaat gttgcaggtg tagatactgt taaatcaagt gccaacacat
    19201 taaatggtgc tatgggtacg ttaagaaata gcatacaaga taacacagct acgaaaaatg
    19261 gccaaaacta tcttgatgct acagaacgta acaaaacaaa ctataacaat gctgttgata
    19321 gtgctaatgg tgtcattaat gcaacaagca atccaaatat ggatgctaat gcaattaacc
    19381 aaatcgctac acaagtgaca tcaacgaaaa atgcattaga tggtacacat aatttaacgc
    19441 aagcgaaaca aacagcaaca aatgccatcg atggtgctac taacttaaat aaagcgcaaa
    19501 aagatgcgtt aaaagcacaa gttacaagtg cgcaacgtgt tgcaaatgta acaagtatcc
    19561 aacaaactgc aaatgaactt aatacagcta tgggtcaatt acaacatggt attgatgatg
    19621 aaaatgcaac aaaacaaact caaaaatatc gtgacgctga acaaagtaag aaaactgctt
    19681 atgatcaagc tgtagctgct gcgaaagcaa ttttaaataa acaaacaggt tcaaattcag
    19741 ataaagcagc agttgaccgt gcattacaac aagtaacaag tacgaaagat gcattgaatg
    19801 gtgatgcaaa actggcagaa gcgaaagcgg cagctaaaca aaacttaggc actttaaacc
    19861 atattacgaa tgcacaacgt actgacttag aaggccaaat caatcaagcg acgactgttg
    19921 atggcgttaa tactgtaaaa acaaatgcca atacattaga cggcgcaatg aatagcttac
    19981 aaggttcaat caatgataaa gatgcgacat taagaaatca aaattatctt gatgcggatg
    20041 aatcaaaacg aaatgcatat acgcaagctg tcacagcggc tgaaggcatt ttaaataaac
    20101 aaactggtgg taacacatct aaagcagacg ttgataatgc attaaatgca gttacaagag
    20161 cgaaagcggc tttaaatggt gctgacaact taagaaatgc gaaaacttca gcaacaaata
    20221 cgattgatgg tttacctaac ttaacacaat tacaaaaaga caacttgaag catcaagttg
    20281 aacaagcgca aaatgtagca ggtgtaaatg gtgttaaaga taaaggtaat acgttaaata
    20341 ctgccatggg tgcattacgt acaagtatcc aaaatgataa tacgacgaaa acaagtcaaa
    20401 attatcttga tgcatctgac agcaacaaaa ataattacaa tactgctgta aataatgcaa
    20461 atggtgttat taatgcaacg aacaatccaa atatggatgc taatgcgatt aatggcatgg
    20521 caaatcaagt caatacaaca aaagcagcgt taaatggtgc acaaaactta gctcaagcta
    20581 aaacaaatgc gacgaacaca attaacaacg cacatgactt aaaccaaaaa caaaaagatg
    20641 cattaaaaac acaagttaac aatgcacaac gtgtatctga tgcaaataac gttcaacaca
    20701 ctgcaactga attgaacagt gcgatgacag cacttaaagc agctattgct gataaagaaa
    20761 gaacaaaagc aagcggtaat tatgtcaatg ctgatcaaga aaaacgtcaa gcgtatgatt
    20821 caaaagtgac taacgctaaa aatcattagt ggtacaccga atgcgacatt aacagtcaat
    20881 gacgtaaata gtgcggcatc acaagtcaat gcggctaaaa cagcattaaa tggtgataac
    20941 aacttacgtg tagcgaaaga gcatgccaac aatacaattg acggcttagc acaattgaat
    21001 aatgcacaaa aagcaaaatt aaaagaacaa gttcaaagtg caactacatt agatggtgtt
    21061 caaactgtta aaaatagttc tcaaacgttg aatacagcga tgaaaggctt aagagatagt
    21121 attgcgaatg aagcaacaat taaagcaggt caaaactaca ctgacgcaag tccaaataat
    21181 cgtaacgagt acgacagtgc agttactgca gcaaaagcaa tcattaatca aacatcgaac
    21241 ccaacgatgg aaccaaatac tattacgcaa gtaacatcac aagtgacaac taaagaacag
    21301 gcattaaatg gtgcgcgaaa cttagctcaa gctaagacaa ctgcgaaaaa caacttgaat
    21361 aacttaacat caattaacaa tgcacaaaaa gatgcgttaa cgcgtagcat tgatggtgca
    21421 acaacagtag ctggtgtaaa tcaagaaact gcaaaagcaa cagaattaaa taacgcaatg
    21481 catagtttac aaaatggtat caatgatgag acacaaacaa aacaaactca gaaataccta
    21541 gatgcagagc caagtaagaa atcagcttat gatcaagcag taaatgcagc gaaagcaatt
    21601 ttaacaaaag ctagtggtca aaatgtagac aaagcagcag ttgaacaagc attgcaaaat
    21661 gtgaacagta cgaagacggc gttgaacggt gatgcgaaat taaatgaagc taaagcagct
    21721 gcgaaacaaa cgttaggtac attaacacac attaataatg cacaacgtac agcgttagac
    21781 aatgaaatta cacaagcaac aaatgttgaa ggtgttaata cagttaaagc caaagcgcaa
    21841 caattagatg gtgctatggg tcaattagaa acatcaattc gtgataaaga cacgacgtta
    21901 caaagtcaaa attatcaaga tgctgatgat gctaaacgaa ctgcttattc tcaagcagta
    21961 aatgcagcag caactatttt aaataaaaca gctggcggta atacacctaa agcagatgtt
    22021 gaaagagcaa tgcaagctgt tacacaagca aatactgcat taaacggtat tcaaaactta
    22081 gatcgtgcga aacaggctgc taacacagcg attacaaatg cttcggactt aaatacaaaa
    22141 caaaaagaag cattaaaagc acaagtaaca agtgcaggac gtgtatctgc agcaaatggt
    22201 gttgaacata ctgcgactga attaaatact gcgatgacag ctttaaagcg tgccattgct
    22261 gataaagctg agacaaaagc tagtggtaac tatgtcaatg ctgatgcgaa taaacgtcaa
    22321 gcatatgatg aaaaagttac agctgccgaa aatatcgtta gtggtacacc aacaccaacg
    22381 ttaacaccag cagatgttac aaatgcagca acgcaagtaa cgaatgctaa gacgcagtta
    22441 aacggtaatc ataatttaga agtagcgaaa caaaatgcta acactgcaat tgatggttta
    22501 acttctttaa atggtccgca aaaagcaaaa cttaaagaac aagtgggtca agcgacgacg
    22561 ttgccaaatg ttcaaactgt tcgtgataat gcacaaacat taaacactgc aatgaaaggt
    22621 ctacgagata gcattgcgaa tgaagcaacg attaaagcag gtcaaaacta cacagatgca
    22681 agtcaaaaca aacaaactga ctacaacagt gcagtcactg cagcaaaagc aatcattggt
    22741 caaacaacta gtccatcaat gaatgcgcaa gaaattaatc aagcgaaaga ccaagtgaca
    22801 gctaaacaac aagcgttaaa cggtcaagaa aacttaagaa ctgcgcaaac aaatgcgaag
    22861 caacatttga acggcttaag tgacttaact gacgctcaaa aagatgcagt gaaacgtcaa
    22921 atcgaaggtg caacgcatgt taatgaagta acacaagcac aaaataatgc ggatgcatta
    22981 aatacagcta tgacgaactt gaaaaatggt attcaagatc agaatacgat taagcaaggt
    23041 gttaacttca ctgatgccga cgaagcgaaa cgtaatgcat atacaaatgc agtgacgcaa
    23101 gctgaacaaa ttttaaataa agcacaaggt ccaaatactt caaaagacgg tgtcgaaact
    23161 gcgttagaaa atgtacaacg tgctaaaaac gaattgaacg gtaatcaaaa tgttgcgaac
    23221 gctaagacaa ctgcgaaaaa tgcattgaat aacctaacat caattaataa tgcacaaaaa
    23281 gaagcattga aatcacaaat tgaaggtgcg acaacagttg caggtgtaaa tcaagtgtct
    23341 acaacggcat ctgaattaaa tacagcaatg agcaacttac aaaatggtat taatgatgaa
    23401 gcagctacaa aagcagctca gaaatatact gatgcagata gagaaaaaca aactgcatac
    23461 aatgacgctg taacagcagc taaaacgtta ttagataaaa cagctggttc aaatgacaat
    23521 aaagcagctg ttgaacaagc attacaacgt gtgaatactg ctaaaacagc attaaatggt
    23581 gacgagcgat taaatgaagc gaagaacaca gctaaacaac aagtagcgac aatgtcacac
    23641 ttaactgatg ctcaaaaagc aaacttaaca tcgcaaatcg aaagtggtac gactgttgca
    23701 ggtgttcaag gtattcaagc taatgccggt actttagatc aagcaatgaa tcaattaaga
    23761 caaagtattg cttctaaaga tgcgactaaa tcaagcgaag attatcaaga cgcgaatgca
    23821 gatttacaaa atgcatacaa tgatgcggta actaatgctg aaggtattat tagtgcaacg
    23881 aataaccctg aaatgaatcc tgatacaatt aaccaaaaag cgagccaagt gaacagtgcg
    23941 aagtctgcat tgaacggtga tgaaaaatta gcagcagcaa aacaaactgc gaaatcagat
    24001 atcggtcgtt tgacagactt gaacaatgca caacgaactg cggcaaatgc tgaagtggat
    24061 caagcaccaa atcttgcagc tgtcacagcg gctaaaaata aagcaacatc gttaaacaca
    24121 gcgatgggta atttgaaaca tgcacttgct gaaaaggata atacgaaacg tagtgtcaat
    24181 tacacagatg cggatcaacc aaaacaacaa gcgtatgata ctgcggttac acaagcagaa
    24241 gcaattacta atgctaatgg cagcaacgcg aatgaaacac aagttcaagc agcactaaac
    24301 caattgaatc aagctaaaaa tgacttgaat ggtgataata aagttgctca agcaaaagag
    24361 tcagcgaaac gtgcattagc ttcatatagt aacttgaata atgcgcaatc aactgcagca
    24421 actagtcaaa ttgacaatgc aacgacagta gcaggcgtaa ctgctgcaca aaatactgct
    24481 aatgaattaa atacagcaat gggtcaactt caaaatggta taaatgacca aaacactgtt
    24541 aaacaacaag tgaactttac agatgctgac caaggtaaga aagatgctta cacaaatgct
    24601 gttacgaatg ctcaaggtat tttagataaa gcacacggtc aaaatatgac gaaagcacaa
    24661 gttgaagctg cattaaatca agtaacgact gctaagaatg ctttaaacgg tgacgcaaat
    24721 gtaagacaag caaaatcaga tgcgaaagca aacttaggta cattaacaca cttaaataat
    24781 gcacaaaaac aagatttaac atcacaaatc gaaggcgcaa caacagtcaa cggtgtaaat
    24841 ggtgttaaaa cgaaagcaca agacttagat ggtgcaatgc aacgattaca atcagctata
    24901 gcaaataaag atcaaactaa agcgagcgaa aattacatcg acgcagatcc aactaagaaa
    24961 acagcatttg ataatgctat cacacaagct gaatcttact taaataaaga tcacggtgcg
    25021 aataaagata agcaagctgt tgaacaagca attcaaagtg taacgtctac tgaaaatgct
    25081 ttgaacggtg acgcgaactt acaacgcgct aaaactgaag ctatacaagc tatcgataac
    25141 ttgacacatt tgaatacacc acaaaaaaca gcattaaaac aacaagtgaa cgctgcgcaa
    25201 cgtgtatcag gtgtaactga tctgaaaaat agtgctacat cacttaataa tgcgatggat
    25261 caattaaaac aagcaattgc tgatcatgac acaattgtag ctagtggtaa ttacactaac
    25321 gcgagtcctg ataagcaagg tgcttatact gatgcatata atgctgcgaa aaacattgta
    25381 aatggttcgc ctaatgtgat tacaaatgca gcagatgtta cagcagcaac acaacgtgtt
    25441 aataatgctg aaacaggttt aaacggtgat acaaacttag caactgcgaa gcaacaagct
    25501 aaagatgcat tacgtcaaat gacacattta tctgatgcac aaaaacaaag tattactggt
    25561 caaattgata gcgcgacaca agtaactggc gttcaaagtg tgaaagacaa cgcgacaaat
    25621 cttgataatg caatgaatca acttcgaaat agtattgcga ataaagatga tgtaaaagcg
    25681 agtcaaccat atgttgatgc agatagagat aaacaaaatg catacaatac agcagttaca
    25741 aatgctgaaa atatcattaa tgcaacgagt cagccgacac ttgatccatc tgcagtaaca
    25801 caagcagcta atcaagtgag cactaacaaa actgcgctta atggtgcaca aaacttagcg
    25861 aataaaaagc aagaaacgac tgctaacatc aaccaattaa gtcatttaaa taatgctcaa
    25921 aagcaagatt taaatacgca agtgacaaat gcaccaaata ttagcacagt aaatcaagtg
    25981 aaaactaaag ctgaacaatt agatcaagca atggaacgtt taatcaacgg aatccaagac
    26041 aaagatcaag tgaaacaaag tgttaacttt acagatgcag atccagaaaa acaaacagca
    26101 tacaacaatg cggtaactgc tgctgaaaat attattaatc aagcaaatgg tacaaatgcg
    26161 aaccaatcac aagttgaagc agcactttca actgtaacaa ctacgaaaca agcgttgaat
    26221 ggtgatagaa aagtaacaga tgctaaaaac aatgcaaacc aaacattatc tacgttagat
    26281 aacttaaaca atgcacaaaa aggtgctgtt actggaaaca tcaatcaagc gcacactgta
    26341 gctgaagtaa cgcaagccat tcaaaccgct caggaactga atacagcgat gggtaacttg
    26401 aaaaatagct tgaatgataa agacactaca cttggcagtc aaaactttgc agatgcagat
    26461 ccagagaaga aaaatgcata caatgaagcg gttcataatg ctgaaaatat tttaaataaa
    26521 tctacaggta cgaacgtgcc taaagatcaa gttgaagcag ctatgaatca agtgaatgct
    26581 acaaaagcag cgcttaatgg tactcaaaac cttgaaaaag ctaaacaaca cgcaaataca
    26641 gcaattgacg gtttaagcca tttaacaaat gcacaaaaag aggcattaaa acaattggta
    26701 caacaatcga ctactgttgc agaagcacaa ggtaatgagc aaaaagcaaa caatgttgat
    26761 gcagcaatgg acaaattacg tcaaagtatt gcagataatg cgacaacaaa acaaaaccaa
    26821 aattatactg atgcaagtca gaataaaaag gatgcgtaca ataatgctgt cacaactgca
    26881 caaggtatta ttgatcaaac tacaagtcca actttagatc cgactgttat caatcaagct
    26941 gctggacaag taagcacaac taaaaatgca ttaaatggta atgaaaacct agaggcagcg
    27001 aaacaacaag cgtcacaatc attaggttca ttagataact taaataatgc gcaaaaacaa
    27061 acagttactg atcaaattaa tggcgcgcat actgttgatg aagcaaatca aattaagcaa
    27121 aatgcgcaaa acttaaatac agcgatgggt aacttgaaac aagcgatagc tgacaaagat
    27181 gctacgaaag cgacagttaa cttcactgat gcagatcaag caaaacaaca agcatataac
    27241 actgctgtta caaatgctga aaatatcatt tcaaaagcta atggcggcaa tgcaacacaa
    27301 gctgaagttg aacaagcaat caaacaagtt aatgctgcaa aacaagcatt aaatggtaat
    27361 gccaacgttc aacatgcaaa agacgaagca acagcattaa ttaatagctc taatgacctt
    27421 aaccaagcac aaaaagacgc attaaaacaa caagttcaaa atgcaactac tgtagctggt
    27481 gtaaacaatg ttaaacaaac agcacaagag ttaaacaatg ctatgacaca attaaaacaa
    27541 ggcattgcag ataaagaaca aacaaaagct gatggtaact ttgtcaatgc agatcctgat
    27601 aagcaaaatg catataatca agcagtagcg aaagctgaag cattaattag tgctacgcct
    27661 gatgttgtcg ttacacctag cgaaattact gcagcgttaa ataaagttac gcaagctaaa
    27721 aatgatttaa atggtaatac aaacttagca acggcgaaac aaaatgttca acatgctatt
    27781 gatcaattgc caaacttaaa ccaagcgcaa cgtgatgaat acagcaaaca aatcacgcaa
    27841 gcaacacttg taccaaacgt caatgctatt caacaagcgg cgacaacgct taatgacgcg
    27901 atgacacaat tgaaacaagg tattgcgaat aaagcacaaa ttaaaggtag cgagaactat
    27961 cacgatgctg atactgacaa gcaaacagca tatgataatg cagtaacaaa agcagaagaa
    28021 ttgttaaaac aaacaacaaa tccaacaatg gatccaaata caattcaaca agcattaact
    28081 aaagtgaatg acacaaatca agcacttaac ggtaatcaaa aattagctga tgccaaacaa
    28141 gatgctaaga caacacttgg tacactagat catttaaatg atgctcaaaa acaagcgcta
    28201 acaactcaag ttgaacaagc accagatatt gcaacagtta ataatgttaa gcaaaatgct
    28261 caaaatctga ataatgctat gactaactta aacaatgcat tacaagataa aactgagaca
    28321 ttaaatagca ttaactttac tgatgcagat caagctaaga aagatgctta tactaatgcg
    28381 gtttcacatg cagaaggtat tttatctaaa gcaaatggca gcaatgcaag tcaaactgaa
    28441 gtggaacaag cgatgcaacg tgtgaacgaa gcgaaacaag cattgaatgg taatgacaat
    28501 gtacaacgtg caaaagatgc agcgaaacaa gtgattacaa atgcaaatga tttaaatcaa
    28561 gcgcaaaaag atgcattaaa acaacaagtc gatgctgcgc aaactgttgc aaatgtaaac
    28621 acgattaagc aaacagcaca agatttaaat caagcaatga cacaattgaa acaaggtatt
    28681 gcagataaag accaaactaa agcaaatggt aactttgtca atgctgatac tgataagcaa
    28741 aatgcttaca acaatgcggt agcacatgct gaacaaataa ttagtggtac accaaatgca
    28801 aacgtggatc cacaacaagt ggctcaagcg ttacaacaag tgaatcaagc taagggtgat
    28861 ttaaacggta accataactt acaagttgct aaagacaatg caaatacagc cattgatcag
    28921 ttaccaaact taaatcaacc acaaaaaaca gcattaaaag accaagtgtc gcatgcagaa
    28981 cttgttacag gtgttaatgc tattaagcaa aatgctgatg cgttaaataa tgcaatgggt
    29041 acattgaaac aacaaattca agcgaacagt caagtaccac agtcagttga ctttacacaa
    29101 gcggatcaag acaaacaaca agcatataac aatgcggcta accaagcgca acaaatcgca
    29161 aatggcatac caacacctgt attgacgcct gatacagtaa cacaagcagt gacaactatg
    29221 aatcaagcga aagatgcatt aaacggtgat gaaaaattag cacaagcgaa acaagaagct
    29281 ttagcaaatc ttgatacgtt acgcgattta aatcaaccac aacgtgatgc attacgtaac
    29341 caaatcaatc aagcacaagc gttagctaca gttgaacaaa ctaaacaaaa tgcacaaaat
    29401 gtgaatacag caatgagtaa cttgaaacaa ggtattgcaa acaaagatac tgtcaaagca
    29461 agtgagaact atcatgatgc tgatgccgat aagcaaacag catatacaaa tgcagtgtct
    29521 caagcggaag gtattatcaa tcaaacgaca aatccaacgc ttaacccaga tgaaataaca
    29581 cgtgcattaa ctcaagtgac tgatgctaaa aatggcttaa acggtgaagc taaattggca
    29641 actgaaaagc aaaatgctaa agatgccgta agtgggatga cgcatttaaa cgatgctcaa
    29701 aaacaagcat taaaaggtca aatcgatcaa tcgcctgaaa ttgctacagt gaaccaagtt
    29761 aaacaaacag caacgagcct agatcaagca atggatcaat tatcacaagc tattaatgat
    29821 aaagctcaaa cattagcgga cggtaattac ttaaatgcag atcctgacaa acaaaatgcg
    29881 tataaacagg cagtagcaaa agctgaagca ttattgaata aacaaagtgg tactaatgaa
    29941 gtacaagcac aagttgaaag catcactaat gaagtgaacg cagcgaaaca agcattaaat
    30001 ggtaatgaca atttggcaaa tgcaaaacaa caagcaaaac aacaattggc gaacttaaca
    30061 cacttaaatg atgcacaaaa acaatcattt gaaagtcaaa ttacacaagc gccacttgtt
    30121 acagatgtca ctacgattaa tcaaaaagca caaacgttag atcatgcgat ggaattatta
    30181 agaaatagtg ttgcggataa tcaaacgaca ttagcgtctg aagattatca tgatgcaact
    30241 gcgcaaagac aaaatgacta taaccaagct gtaacagctg ctaataatat cattaatcaa
    30301 actacatcgc ctacgatgaa tccagatgat gttaatggtg caacgacaca agtgaataat
    30361 acgaaagttg cattagatgg tgatgaaaac cttgcagcag ctaaacaaca agcaaacaac
    30421 agacttgatc aattagatca tttgaataat gcgcaaaagc aacagttaca atcacaaatt
    30481 acgcaatcat ctgatattgc tgcagttaat ggtcacaaac aaacagcaga atctttaaat
    30541 actgcgatgg gtaacttaat taatgcgatt gcagatcatc aagccgttga acaacgtggt
    30601 aacttcatca atgctgatac tgataaacaa actgcttata atacagcggt aaatgaagca
    30661 gcagcaatga ttaacaaaca aactggtcaa aatgcgaacc aaacagaagt agaacaagct
    30721 attactaaag ttcaaacaac acttcaagcg ttaaatggag accataattt acaagttgct
    30781 aaaacaaatg cgacgcaagc aattgatgct ttaacaagct taaatgatcc tcaaaaaaca
    30841 gcattaaaag accaagttac agctgcaact ttagtaactg cagttcatca aattgaacaa
    30901 aatgcgaata cgcttaacca agcaatgcat ggtttaagac agagcattca agataacgca
    30961 gcaactaaag caaatagcaa atatatcaac gaagatcaac cagagcaaca aaactatgat
    31021 caagctgttc aagccgcaaa taatattatc aatgaacaaa ctgcaacatt agataataat
    31081 gcgattaatc aagcagcgac aactgtgaat acaacgaaag cagcattaca tggtgatgtg
    31141 aagttacaaa atgataaaga tcatgctaag caaacggtta gtcaattagc acatctaaac
    31201 aatgcacaaa aacatatgga agatacgtta attgatagtg aaacaactag aacagcagtt
    31261 aagcaagatt tgactgaagc acaagcatta gatcaactta tggatgcatt acaacaaagt
    31321 attgctgaca aagatgcaac acgtgcgagc agtgcatatg tcaatgcaga accgaataaa
    31381 aaacaatcct atgatgaagc agttcaaaat gctgagtcta tcattgcagg attaaataat
    31441 ccaactatca ataaaggtaa tgtatcaagt gcgactcaag cagtaatatc atctaaaaat
    31501 gcattagatg gtgttgaacg attagctcaa gataagcaaa ctgctggaaa ttctctaaat
    31561 catttagatc aattaacacc agctcaacaa caagcgctag aaaatcaaat taataatgca
    31621 acaactcgtg ataaagtggc tgaaatcatt gcacaagcac aagcattaaa tgaagcgatg
    31681 aaagcattaa aagaaagtat taaggatcaa ccacaaactg aagcaagtag taaatttatt
    31741 aacgaggatc aagcgcaaaa agatgcttat acgcaagcag tacaacacgc gaaagatttg
    31801 attaacaaaa caactgatcc tacattagct aaatcaatca ttgatcaagc gacacaggca
    31861 gtgacagatg ctaaaaacaa tttacatggt gatcaaaaac tagctcaaga taagcaacgt
    31921 gcaacagaaa cgttaaataa cttgtctaac ttgaatacac cacaacgtca agcacttgaa
    31981 aatcaaatta ataatgcagc aactcgtggc gaagtagcac aaaaattaac tgaagcacaa
    32041 gcacttaacc aagcaatgga agctttacgt aatagcattc aagatcaaca gcaaacggaa
    32101 gcgggtagca agtttatcaa tgaagataaa ccacaaaaag atgcttacca agcagcagtt
    32161 caaaatgcaa aagatttaat taatcaaact aacaatccaa cgcttgataa agcacaagtt
    32221 gaacaattga cacaagctgt taaccaagct aaagataacc tacacggtga tcaaaaactt
    32281 gcagacgata aacaacatgc ggttactgat ttaaatcaat taaatggttt gaataatccg
    32341 caacgtcaag cacttgaaag ccaaataaac aacgcagcaa ctcgtggcga agtagcacaa
    32401 aaattagctg aagcaaaagc gcttgatcaa gcaatgcaag cattacgtaa tagtattcaa
    32461 gatcaacaac aaacagaatc tggtagcaag tttatcaatg aagataaacc gcaaaaagat
    32521 gcttaccaag cagcagttca aaatgcaaaa gatttaatta accaaacagg taatccaaca
    32581 ctcgacaaat cacaagtaga acaattgaca caagcagtaa caactgcaaa agataatcta
    32641 catggtgatc aaaaacttgc tcgtgatcaa caacaagcag taacaactgt aaatgcattg
    32701 ccaaacttaa atcatgcaca acaacaagca ttaactgatg ctataaatgc agcgcctaca
    32761 agaacagagg ttgcacaaca tgttcaaact gctactgaac ttgatcacgc gatggaaaca
    32821 ttgaaaaata aagttgatca agtgaataca gataaggctc aaccaaatta cactgaagcg
    32881 tcaactgata aaaaagaagc agtagatcaa gcgttacaag ctgcagaaag cattacagat
    32941 ccaactaatg gttcaaatgc gaataaagac gctgtagacc aagtattaac taagcttcaa
    33001 gaaaaagaaa atgagttaaa tggtaatgag agagtcgctg aagctaaaac acaagcgaaa
    33061 caaactattg accaattaac acatttaaat gctgatcaaa ttgcaactgc taaacaaaac
    33121 attgatcaag cgacgaaact tcaaccaatt gctgaattag tagatcaagc aacgcaattg
    33181 aatcaatcta tggatcaatt acaacaagca gttaatgaac atgctaacgt tgagcaaact
    33241 gtagattaca cacaagcaga ttcagataaa caaaatgctt ataaacaagc tattgctgat
    33301 gctgaaaatg tattgaaaca aaatgcgaat aagcaacaag tggatcaagc acttcaaaat
    33361 attttaaatg caaaacaagc attaaatggt gatgaacgtg tagcacttgc taaaacaaat
    33421 ggtaaacatg acatcgacca attgaatgca ttaaacaatg ctcaacaaga tggatttaaa
    33481 ggtcgcatcg atcaatcaaa cgatttaaat caaatccaac aaattgtaga tgaggctaag
    33541 gcacttaatc gtgcaatgga tcaattgtca caagaaatca ctgacaatga aggacgcacg
    33601 aaaggtagca cgaactatgt caatgcagat acacaagtca aacaagtata tgatgaaacg
    33661 gttgataaag cgaaacaagc acttgataaa tcgactggtc aaaacttaac tgcaaaacaa
    33721 gttatcaaat taaatgatgc agtcactgca gctaagaaag cattaaatgg tgaagaaaga
    33781 cttaataatc gtaaagctga agcattacaa agattggatc aattaacaca tctaaacaat
    33841 gctcaaagac aattagcaat ccaacaaatt aataatgctg aaacgctaaa taaagcatct
    33901 cgagcaatta atagagcaac taaattagat aatgcaatgg gtgcagtaca acaatatatt
    33961 gacgaacagc accttggtgt tatcagcagc acaaattaca tcaatgcaga tgacaatttg
    34021 aaagcaaatt atgataatgc aattgcgaat gcagcacatg agttagataa agtgcaaggt
    34081 aatgcaattg caaaagctga agcagagcaa ttgaaacaaa atattatcga tgctcaaaat
    34141 gcattaaatg gagaccaaaa ccttgcaaat gccaaagata aagcaaatgc gtttgttaat
    34201 tcgttaaatg gattaaatca acagcaacaa gatcttgcac ataaagcaat taacaatgcc
    34261 gatactgtat cagatgtaac agatattgtt aataatcaaa ttgacttaaa tgatgcaatg
    34321 gaaacattga aacatttagt tgacaatgaa attccaaatg cagagcaaac tgtcaattac
    34381 caaaacgctg acgataatgc taaaacaaac ttcgatgatg ccaaacgtct agcaaataca
    34441 ttgctaaata gtgataacac aaatgtgaat gatatcaatg gcgcaatcca agcagtcaat
    34501 gatgcaatcc ataatcttaa tggtgatcaa cgactacaag atgctaaaga caaggcaatt
    34561 caatctatta atcaagcttt agctaataag ctaaaagaaa tcgaagcttc aaatgcgacg
    34621 gatcaagaca agcttattgc gaaaaataaa gcagaagaat tggcaaacag catcatcaac
    34681 aacattaata aagcaacaag taatcaggct gtatctcaag ttcaaacagc aggcaaccac
    34741 gcgattgaac aagtgcatgc caatgaaata ccaaaagcaa aaattgatgc caataaagac
    34801 gttgataagc aagttcaagc attaattgac gaaattgatc gaaatccaaa tctaacagat
    34861 aaggaaaaac aagcacttaa agatcgtatt aatcaaatac ttcaacaagg tcataacggc
    34921 attaacaatg cgatgactaa agaagaaatt gaacaagcca aagcacaact tgcgcaagca
    34981 ttacaagaca tcaaagattt agtgaaagct aaagaagatg cgaaacaaga tgttgataaa
    35041 caagttcaag ctttaattga cgaaatcgat caaaatccaa atctaacaga taaggaaaaa
    35101 caagcactta aagatcgaat taatcaaata cttcaacaag gtcataacga cattaacaat
    35161 gcgatgacaa aagaagcaat tgaacaagca aaagaacgtt tagcgcaagc attgcaagac
    35221 atcaaagatt tagtgaaagc taaagaagat gcgaaaaatg atattgataa acgtgtacaa
    35281 gctttaattg acgaaatcga tcaaaatcca aatctaacag ataaggaaaa acaagcactt
    35341 aaagatcgaa ttaatcaaat acttcaacaa ggtcataacg acattaacaa tgcgctgact
    35401 aaagaagaaa ttgagcaggc aaaagcacaa cttgcacaag cattgcaaga catcaaagat
    35461 ttagtgaaag ctaaagaaga tgcgaaaaat gcaataaaag ccttagctaa tgcgaagcgt
    35521 gatcaaatca attcaaatcc agatttaaca cctgagcaaa aagcaaaagc gctcaaagaa
    35581 attgacgaag ctgaaaaacg agcactacaa aacgttgaga atgctcaaac tatagatcaa
    35641 ttaaatcgag gattaaactt aggtttagat gacattagaa atacacatgt atgggaggtt
    35701 gatgaacaac ctgctgtaaa tgaaattttt gaagcaacac ctgagcaaat cctagttaat
    35761 ggtgaactca ttgtacatcg tgatgacatc attacagaac aagatattct tgcacacata
    35821 aacttaattg atcagctttc agcagaagtc atcgatacac catcaactgc aacgatttct
    35881 gatagcttaa cagcaaaagt tgaagttaca ttgcttgatg gatcaaaagt gattgttaat
    35941 gttcctgtaa aagttgtaga aaaagaattg tcagtagtca aacaacaggc aattgaatca
    36001 atcgaaaatg cggcacaaca aaagattaat gaaatcaata atagtgtgac attaacactg
    36061 gaacaaaaag aagctgcaat tgcagaagtt aataagctta aacaacaagc aattgatcat
    36121 gttaacaatg catctgatgt tcattcagtt gaagaaattc aacaacaaga acaagcgcat
    36181 attgaacaat ttaatccaga acaatttacg attgaacaag caaaatcaaa tacaattaaa
    36241 tcgattgaag atgcaattca acatatgatt gatgaaatca aagctcgtac tgatctaaca
    36301 gataaagaga agcaagaagc tattgctaag ttaaatcaat taaaagaaca agcaattcaa
    36361 gcgattcaac gtgcgcaaag catcgatgaa ataagtgagc aattggaaca atttaaagct
    36421 caaatgaaag cagctaatcc aacagcaaaa gaactagcta aacgcaagca agaagctatt
    36481 agtagaatta aagacttttc aaatgaaaaa ataaatagta ttcgaaatag tgaaattggc
    36541 acagctgatg aaaaacaagc agcaatgaat caaattaacg aaattgtgct tgaaacaatt
    36601 agagatatta ataatgcgca tacattacag caagttgagg ctgcattgaa caatggtatt
    36661 gctcgaattt cagcagtaca aattgtaaca tctgatcgtg ctaaacaatc gtcaagtact
    36721 ggaaatgaat ctaatagcca tttaacaatt ggttatggaa ctgcaaatca tccatttaac
    36781 agttcgacta ttggacataa aaagaaactt gatgaagatg atgacattga tccacttcat
    36841 atgcgtcact ttagtaataa tttcggtaat gttattaaaa acgctattgg tgtggtgggt
    36901 atctctggtt tactagctag tttctggttc ttcattgcca aacgtcgtcg taaagaagat
    36961 gaagaagatg tggaagttac aaatgaaaac acagatgaaa aagtgttgaa agataacgaa
    37021 cattcaccac tcttattcgc aaaacgacgc aaagataaag aggaagatgt tgaaacaaca
    37081 actagtattg aatctaaaga tgaggacgtt cctttattat tggctaaaaa gaaaaatcaa
    37141 aaagataacc aatccaaaga caaaaagtca gcatcaaaaa atacttctaa aaaggtagca
    37201 gctaaaaaga agaaaaagaa agctaagaaa aataaaaaat aatttgtttc tttgataaat
    37261 agaggagcac cgattgacat cacatcagtc ggtgctcctt ttatttattc tttttaatta
    37321 atttatacaa tgcctgttga gcgtgttgat tcgcttcttt gttttgttct ctcggtatcc
    37381 atttaacaaa taataaatca aaatcttttt caaatatttc tatttgatca aaataaggtt
    37441 tgaaatttgc gtttttcaca taaccagctt caatgctatc tgcaattagc tttgagtctg
    37501 tatataatag tgcgttttga acatttaatt cacgtgcatg ttctagtgca taaatacatg
    37561 cagcccattc tgcagtgtgg ttatccattt cgcctaactc atgtgtatat gtataatgct
    37621 gctcatcttc tttgattaca atggcacatg tacttatgcc tggatttcct ttcgtcgcag
    37681 catcaaaatt tattttcgcc ataataaacc tactttctat tcaatactta gttaaagtta
    37741 ctattactgt aatacaaaat atgttgggta atccattaaa aaacacgcat cacttaaata
    37801 agtaacacgt gtttaaaata ctcgctgatt caaagatgat tttctaatac gtagtactgt
    37861 aatatacttc ctaaaaaaat catcttcagg ctgggacata aatcaatgtt ctatgctcta
    37921 cgatgttata ttggcagtag ttgactgaac gaaaatgcgc ttgtaacaag cttttttcaa
    37981 ttctagtcag gggccccaac acagagaatt tcgaaaagaa attctacagg caatgcgagt
    38041 tggggtgtgg gtcccaacac agaagatgac gaaaagtcag cttacaataa tgtgcaagtt
    38101 tgggatgggc cccaacaaag agaaattgga ttcccaattt ctacagacaa tgcaagttgg
    38161 ggtgggacga cgaaataaat tttgcgaaaa tattatttct gtcccactcc cttaaaactt
    38221 attcttttgt gtagtaagtg cgttaatagc cttgatctaa cttatcaatc ttacctttac
    38281 gataaaatga tttagcaata tatccaaatg gtacattgaa aactgttgaa gctaatttta
    38341 atacgtacgt tgtaataaat atttcaaata caactgtacc aggtaaactt ccgataaatg
    38401 cgatagctac aaataaagct gtatcaatta ttgagcttaa aaatgtacta ccatatgcac
    38461 ggatgaaaaa cgttttatca gaactaaata cttttttaat tagtgaaaag ataaatacat
    38521 caatatgttg accaataata tatgcgacga ttgagcctaa agcaatgcgt ggcacaacat
    38581 caaagattgc gtgtaatgct ttttgtgcca tatcttctgg tgcaggaata aaatgtaatg
    38641 acaattgcat aacaataatc ataattaatg ttgatgaaaa gcctaaccaa actgctcttt
    38701 ttgcaactct acgcccataa atatcgttta atatatctgt tgctaaataa atagaagcaa
    38761 acatgacatt acctaaagtt gctgaaatac caaagatttc tacagtttta atcacttgta
    38821 tgttggcaat gattgtgcca attgcaaccc atgcaattaa accttgttta ccaaaaaagc
    38881 gatacataag taccataagc acgaacgttg caataaacgt aactagtcct aaaatttcat
    38941 tatacatatt aaaatgtcct cctaaatttt gatcatgcgg gtgtttagaa accgctcaat
    39001 aaataactaa ctcattttac tgaaaaatcg tttgcgatgc aatagcattt gaatataaat
    39061 acattttacg tatagaattg taaaaggttt cattcaaatc ttagggtcaa aaatgttata
    39121 atatttttat gtcaaattta aaacagtaac acttatttac aaggttgcaa tattttgaag
    39181 taataaagga agtgtcgcgt attttaactt tttcagagca aaatgcactc gcgaaaatag
    39241 atgatttaat gaatacttat tgcaatcaat gtccaatcaa aactcgtctg cgtaaattag
    39301 aggggaaaac gaaggcgcat catttttgta tcaatgagtg ttcaataggg aaagaaataa
    39361 aacaattagg aaatgaactt caataggagg aagtcaaatg aaaattatat ctatatcaga
    39421 aacaccgaac cacaacacaa tgaagattac acttagtgaa agcagagaag gtatgacatc
    39481 agatacgtat actaaagttg atgattcaca gccagcattt attaatgaca tcttaaaggt
    39541 tgaaggcgtt aaatcaattt tccatgttat ggactttatt tcagtagata aagaaaatga
    39601 cgcaaattgg gaaacagtat tgccaaaagt agaggctgta ttcgaataaa tttttcatca
    39661 actagtattc ggggggaata aagtatatgg aaattttacg tatagagcca acaccaagtc
    39721 caaatacaat gaaagttgtt ttgtcatata caagagaaga caagttatct aatacttata
    39781 aaaaagtaga agaaacacaa ccaagattta taaatcagtt gttatctata gatggtatca
    39841 cttccatttt tcatgtcatg aacttcttag ctgttgataa ggcaccaaaa gctgattggg
    39901 aagtcatatt acctgatatt aaagctgctt tttctgatgc gaataaggtt ttagaatctg
    39961 taaatgaacc tcaaattgac aatcattttg gtgaaattaa agctgaatta ttaactttta
    40021 agggtatacc gtatcaaatt aagctaactt ctgctgacca agaattaaga gaacaattac
    40081 cacaaacata tgttgaccat atgactcaag cgcaaacagc acatgacaat attgttttta
    40141 tgcgtaaatg gctagattta ggaaatcgct atggaaatat tcaagaagta atggatggtg
    40201 tcctagaaga agtgctagct acctatccag aatcacagtt acccgtattg gtaaaacatg
    40261 ctttagaaga aaatcacgca actaataatt atcatttcta tcgacatgtc tctttggatg
    40321 aatatcatgc aactgataat tggaagactc gattacgaat gttaaaccat tttccaaagc
    40381 cgacttttga agatataccg ctgcttgatt tagctttatc tgatgaaaaa gtaccggtta
    40441 gacgtcaagc gattgtatta ttaggtatga ttgaaagtaa agaaatttta ccgtatttat
    40501 ataaggggct tcgtgataaa agtcctgctg taagaagaac agcaggggat tgcataagcg
    40561 atttagggta tccagaggca ctaccagaaa tggtgctact attagatgat ccacagaaaa
    40621 tcgttaggtg gcgtgctgct atgtttatct ttgatgaagg taatgcagag cagcttcccg
    40681 cactaaaagc ccatattaat gacaatgcgt ttgaagttaa attacaaatt gaaatggcca
    40741 tatctcgtat tgaaaacgga gatgaagcac ttggttcagt atggaaacaa atggcaaaca
    40801 gaacaattta attggaggaa ttaaatatga atgcatatga tgcttatatg aaagaaattg
    40861 cgcaacaaat gcgtggcgaa ttaactcaaa atggttttac aagtttagaa acgagcgaag
    40921 cagtatcgga gtatatgaac caagtaaatg ctgatgacac tacttttgta gttattaact
    40981 ctacatgcgg ctgtgcagct ggattagcaa gaccagctgc agtagcagtt gcaacacaaa
    41041 atgaacatag acctacaaat acagttacag tttttgctgg gcaagataaa gaagcaactg
    41101 ctacaatgcg agaattcatt cagcaagcac catctagtcc ttcgtatgct ttattcaaag
    41161 gtcaagattt agtttatttt atgcctagag aatttatcga aggtagagat attaatgaca
    41221 ttgcaatgga cttaaaggat gcctttgacg aaaattgtaa atagtacaca taaataaata
    41281 taaaggttaa cacattttat aatattaaaa atggtgtctg tcattgaaaa tagagaatat
    41341 agttgtattc tatttgttaa ataaagtccg tttttaccaa ctatattttc tagaaattta
    41401 actgttttaa taggacatca aacataatat tcaaatcatg tgttaacctc ttttttaaaa
    41461 ttttttagca ttaaagttat agatttgggt aaacaattac caattggaaa catatatcac
    41521 gttacgatgg ggtaggtact taatcagcat tttataaata aagtaacgga attcatgata
    41581 ttaatatcat attcctaaaa tgagtgataa caaaatgcta cataaagtta agttatatca
    41641 aactaaatat acatactata aataatgaaa atgaggtgtt atcgcatatg ttgaattcat
    41701 ttgatgcagc atatcacagt ctttgtgaag aagttttaga aataggaaat acacgaaatg
    41761 atcgcacaaa tacaggtacg atttcgaaat ttggtcatca acttcgcttt gacttatcta
    41821 aaggatttcc actattaacg acaaagaaag tttcttttaa attagtagca accgaattat
    41881 ta
//
LOCUS       DAXWFK010000005        41579 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.005, whole genome shotgun sequence.
ACCESSION   DAXWFK010000005 DAXWFK010000000
VERSION     DAXWFK010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 41579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41579
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.005"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            133..945
                     /gene="lpl10"
                     /locus_tag="ACGEXS_000151"
     CDS             133..945
                     /gene="lpl10"
                     /locus_tag="ACGEXS_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl10"
                     /protein_id="HFF8011202.1"
                     /translation="MEYIKKIALYMSVLLLIIFIGGCGNMKDEQKKEEQTNKTDSKEE
                     QIKKSFAKTLDMYPIKNLEDLYDKEGYRDGEFKKGDKGTWTILTGFSKSNKPGVLDDE
                     GMVLYLNRNTKKATGYYFVNKVYDDISKNHNEKKYRVELKNNKIVLLDNVEDKKLKQK
                     IENFKFFSQYADFKDLKNYQDGNITTNENVPSYEAQYKMNNSDKNVKKLREIYPITTN
                     NSPNLKLYIDGDIKGSSVGYKKIEYKFSKDKGQETTLRDYLNFGPSEGENVE"
     gene            935..2263
                     /locus_tag="ACGEXS_000152"
     CDS             935..2263
                     /locus_tag="ACGEXS_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011203.1"
                     /translation="MLSRKYKIDLKAINQNTESTSAISKASYEVENANNNGLSKRDVI
                     NQFNDLKKMKKFPSNLEYVDSYTDSLTGVTTSAFLNKDTGKVTLGMTGTNLQDEAFKK
                     LKEGEFSRQNVTNALETVKDGYADLKILYSPASDQNYRYANTQEFINKIKSKYDIDFI
                     TGHSLGGRDAVVLGMSNGIPNIVVYNPAPISITSLNPNSPDGKRLLELYKNYKGNITR
                     FVAENDALTENLKKYKHYVFFGNDKVFKNGKGHEMEGFLTEEEQKAIKKELKKLQGYA
                     EENNKSFVKNSNNAISKLASIELLRANMMTTNGGGLSSSQQKVLESLTALTIAQSFSQ
                     LIDDEINQIKKMYNEKKKKFGKNWEDAQKAGKAVGEDLSVNGVLNALDEGQVNESSMV
                     REPEQMISAKERQLSTIGSSVSNYIMRVRLSINEIVDKDQVLASQIGGLL"
     gene            2260..2583
                     /locus_tag="ACGEXS_000153"
     CDS             2260..2583
                     /locus_tag="ACGEXS_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011204.1"
                     /translation="MMFNQINNKNELEESYESEKKRIENELQNLNELRHRTRKENERS
                     YDVFQYLKHEMNYSEDAQRKMTRNIEAYEQEINEIIRKQEWKLEEYKEDLKKSYEKQL
                     DKLSD"
     gene            2602..2916
                     /locus_tag="ACGEXS_000154"
     CDS             2602..2916
                     /locus_tag="ACGEXS_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011205.1"
                     /translation="MTLIEPDMTLRMPDISTTVETLNLISKMEAQKENIRTVIAPEHK
                     HKYKDIENGLKGEEKVLIEQMAQHCEAFKANFKGAAQGDWVKSAMSEIDSIKDDLKKI
                     NS"
     gene            complement(3206..3466)
                     /locus_tag="ACGEXS_000155"
     CDS             complement(3206..3466)
                     /locus_tag="ACGEXS_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011206.1"
                     /translation="MSALLYNGDKDISHERESRCINMLFVILVLYVTGIAFILLSVFG
                     SKTEGLSTKHTLYTIGSAIITIAIFISIGYAIQYLTAALYGL"
     gene            3585..4787
                     /locus_tag="ACGEXS_000156"
     CDS             3585..4787
                     /locus_tag="ACGEXS_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFF8011207.1"
                     /translation="MAKIPVTVLSGYLGSGKTTLLNHILQNREGRRIAVIVNDMSEVN
                     IDKDLVADGGGLSRTDEKLVELSNGCICCTLRDDLLKEVERLVKKGGIDQIVIESTGI
                     SEPVPVAQTFSYIDDELGIDLTAICRLDTMVTVVDANRFVHDINSEDLLMDRDQSVDE
                     TDERSIADLLIDQVEFCDVLIINKIDLISEEELAKLEKMLSALQPTAKIIKTTNSEVD
                     LKEVLNTQRFDFEKASESAGWIKELESGGHASHTPETEEYGISSFVYKRRLPFHAKRF
                     NDWLESMPNNVVRSKGIVWLAQYNHVACLLSQAGSSCNIHPVTYWVASMSEAQQTQIL
                     AERQDVAAEWDPEYGDRHTQFVIIGTELDEEKLTKELDACLVNAQEIDADWQQFEDPY
                     QWQIRPAR"
     gene            complement(5446..5508)
                     /locus_tag="ACGEXS_000157"
     CDS             complement(5446..5508)
                     /locus_tag="ACGEXS_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-4"
                     /protein_id="HFF8011208.1"
                     /translation="MAIVGTIIKIIKAIIDIFAK"
     gene            complement(5572..5640)
                     /locus_tag="ACGEXS_000158"
     CDS             complement(5572..5640)
                     /locus_tag="ACGEXS_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-3"
                     /protein_id="HFF8011209.1"
                     /translation="MEFVAKLFKFFKDLLGKFLGNN"
     gene            complement(5692..5757)
                     /locus_tag="ACGEXS_000159"
     CDS             complement(5692..5757)
                     /locus_tag="ACGEXS_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-2"
                     /protein_id="HFF8011210.1"
                     /translation="MGIIAGIIKFIKGLIEKFTGK"
     gene            complement(5789..5854)
                     /locus_tag="ACGEXS_000160"
     CDS             complement(5789..5854)
                     /locus_tag="ACGEXS_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-1"
                     /protein_id="HFF8011211.1"
                     /translation="MGIIAGIIKVIKSLIEQFTGK"
     gene            6375..7859
                     /locus_tag="ACGEXS_000161"
     CDS             6375..7859
                     /locus_tag="ACGEXS_000161"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase subunit 5"
                     /protein_id="HFF8011212.1"
                     /translation="MLSFQLLFSLFVIALIIALISGLLFLAPVMPMRYIKLHLYILVM
                     PVLFAVIGFFGIHGQHVLGPFKIDRLSWLLAGFVMALGFIIQKFSMRYLLGDHHYRHY
                     FPLFTAITSFASLAWMSEDLRLMALCWGITLLCLTLLMNVNRFWKVPRESAKLSSMTF
                     LCGWLAFVGAIVTIYIATGEWRVPQHIVHPTWSLLTNVLLVLAVMIPAAQFPFHRWLI
                     ESVTAPTPVSAIMHAGIVNAGGVILTRFAPIFDNGFALSLLLILSSISVLLGSGISLV
                     QVDYKRQLVGSTMSQMGFMLVQCALGVYSAAIIHLILHGIFKATLFLQSGSIVKRFNI
                     PKQASAKDAYGWIVMGRVLAIIVAFLFWMSSDRSAYEVLSALILGWSLLVSWNQMVAF
                     SKGRMARLVGMILIAIVTFIYVITHNYFYAVLQNITTHATTPPTVSVIISVVILIFGS
                     LLSIWVARHRYSKAFAVLYVWLVNLGEARSKAIESHPNYLKKYL"
     gene            7872..10577
                     /locus_tag="ACGEXS_000162"
     CDS             7872..10577
                     /locus_tag="ACGEXS_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2309 domain-containing protein"
                     /protein_id="HFF8011213.1"
                     /translation="MTTQLNINSVIENAKRVITPLSPISIFAARNPWEGLEADTFEDV
                     AKWLRDVRDVDIFPNKALIESAVARGELDESVFNQLVTDMLLEHHYNIPQHYINLYID
                     NIKTLKDVPASYMNHSNVDVVADLLLEKSKRDMAESYHHYDVRPMSDAIIDEQGEPLS
                     EQVNRQMIKWTKLYIDQFLSSWTMPKREQSFYHAWLHLAQHDHSFTKAQRQVIKGLPN
                     DPEMTIESVLTHFSIDQEDYQAYVEGHLLALPGWAGMLYYRSQQHHFEQHLLTDYLAI
                     RLVVEQLLVGDEFKSVAKDCESRSENWFKQTVASWCYYSDMPSDVLLQHDVNEIQTFI
                     HFAATMNKNVFKNLWLIAWEMTYESQLKQKIKAGHESVAGALDVNQVNVSENDNANQP
                     HSVLLNDTQAVDENNSELNQMGTSTKAQIAFCIDVRSEPFRRHIEAAGPFETIGIAGF
                     FGLPIQKDAVDEQFKHDSLPVMVPPAYRIKEFADRYDMNVYRQQQQTMSSMFYTFKLM
                     KNNVMPSLLLPELSGPFLSLSTIVNSIMPRKSRASLQKIKQKWLKKPETKLTIDREFD
                     RTSDLPVGFTEQEQIDFALQALKLMDLTEAFAPFVVLAGHASHSHNNPHHASLECGAC
                     GGASSGFNAKLLAMICNRPNVRQGLKQSGVYIPETTVFAVAEHHTSTDTLAWVYVPDT
                     LSSIALDAYESLNDAMPMISEHANRERLDKLPTIGRVNHPVEEAQRFASDWSEVRPEW
                     GLAKNASFIIGRRQLTKGIDLEGRTFLHNYDWRKDKDGTLLNTIISGPALVAQWINLQ
                     YYASTVAPHFYGSGNKATQTVTSGVGVMQGNASDLMYGLSWQSVMAADRTMYHSPIRL
                     LVVIQAPDYVVARLLANNEHFARKVSNHWLRLMSVNEEGRFKSWI"
     gene            10739..11101
                     /locus_tag="ACGEXS_000163"
     CDS             10739..11101
                     /locus_tag="ACGEXS_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HFF8011214.1"
                     /translation="MKRTKGEIEAEISKAITQWEKDFLGRGSLSVKSDILRDMVIISL
                     QGILTPAEYRVCSTNEGLLNIKRTRSELVESGEQDLNDIIFKITGIKVMSFHSDLSTV
                     TGERIIVFKLEDNLEKHI"
     gene            complement(11330..11746)
                     /locus_tag="ACGEXS_000164"
     CDS             complement(11330..11746)
                     /locus_tag="ACGEXS_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011215.1"
                     /translation="MLSIISMCHKFHFTDNIKIIKFILLCILQIKHCIIEITIFCYFQ
                     FSTIYIYYTEGVRRINRVIFVYIALIITLVRFFTPIHPSFSNLIYWIFVLYFIPIILC
                     VIGFKAEKLIATMVIIPNFLGILYRLYAYVTHILFM"
     gene            11784..12458
                     /locus_tag="ACGEXS_000165"
     CDS             11784..12458
                     /locus_tag="ACGEXS_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFF8011216.1"
                     /translation="MIDKKLTSPKMTVPLFLIALIVFIGMFYSVVTNQEWLKNIDMGS
                     LTWFTDYFGEPQRQYVNNLFNYYMTFSAEIGDVKGVVLISIIVTIILFIKQRHLAVWF
                     VTYLVSGVIMNKLIKDTVLRPRPYNHLAVDTGFSFPSGHSNASTLLYFALMIIIISLA
                     AKTITKVLSALVMGILWLSILFCRLYFHAHYFSDVIGGTSLAIIWVALFLMVYPYFIN
                     HRRQRV"
     gene            12495..13229
                     /locus_tag="ACGEXS_000166"
     CDS             12495..13229
                     /locus_tag="ACGEXS_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFF8011217.1"
                     /translation="MRIKTPSPSYLKGTNGHAILLLHSFTGTNRDVKHLAAELNDQGF
                     SCYAPNYPGHGLLLKDFMTYNVDDWWEEVEKAYQFLVNEGYESISATGVSLGGLMTLK
                     LAQHYPLKRIAVMSAPKEKSDDGLIEHLVYYSQRMSNILNLDQQASSAQLAAIDDYEG
                     EITKFQHFIDDIMTNLNVIKMPANILFGGKDAPSYETSAHFIYEHLGSVDKELNGLKD
                     SHHLMTHGEGRDILEENVIRFFNALT"
     gene            13407..13520
                     /locus_tag="ACGEXS_000167"
     CDS             13407..13520
                     /locus_tag="ACGEXS_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011218.1"
                     /translation="MGIAPEVVMWRDVGDIDGVVCGEILAHVYSVEPENLM"
     gene            13685..15022
                     /locus_tag="ACGEXS_000168"
     CDS             13685..15022
                     /locus_tag="ACGEXS_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HFF8011219.1"
                     /translation="MKRQQSQWKSSTGFILASAGSAIGLGAMWKFPYMAGIYGGGAFL
                     AMFLIFTIFVGLPLLIMEFTVGKMGRTYTTQIYSKLTGKKWLNIIGWNGNLAVFILFG
                     FYSVIGGWIVIYIGQVLWQLVIFQRINHLQEMNFEAVISNPWLTVLGQGIFIFATMII
                     VMLGVEKGLEKASKVMMPLLFVFLIVIVIKSLTLDGALEGVKFILQPRVSEITADGIL
                     FALGQSFFTLSLGTTGMITYASYASKDMTIKSSAISIVVMNIFVSVLAGLAIFPALHS
                     FGYEPQEGPGLLFKVLPMVFSQMHLGTLFYLGFLVLFLFAALTSSISLLELNVSNFTK
                     NDNTKRKKVAVIGSILVFIISIPATLSFGILKDVRFGAGTIFDNMDFIVSNVLMPLGA
                     LGTTLVVGQLLDKKLLQQYFGKDRFRLFSGWYYLIKYAMPVVIILVFIVQLFS"
     gene            15239..16153
                     /locus_tag="ACGEXS_000169"
     CDS             15239..16153
                     /locus_tag="ACGEXS_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFF8011220.1"
                     /translation="MITYDLIGNTPLVLLEHYSDDKVKIYAKLEQWNPGGSVKDRLGK
                     YLVEKAIQEGRVRAGQTIVEATAGNTGIGLAIAANRHHLKCKIFAPYGFSEEKINIMI
                     ALGAEVSRTSQSEGMHGAQLAARSYAEKYGAVYMNQFESEHNPDTYFHTLGPELTSAL
                     QQIDYFVAGIGSGGTFTGTARYLKQHHVQCYAVEPEGSVLNGGPAHAHDTEGIGSEKW
                     PIFLERRLVDGIFTIKDQDAFRNVKSLAINEGLLVGSSSGAALQGALNLKAQLSEGTI
                     VVVFPDGSDRYMSKQIFNYEENNNEQEN"
     gene            16137..17279
                     /locus_tag="ACGEXS_000170"
     CDS             16137..17279
                     /locus_tag="ACGEXS_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional cystathionine
                     gamma-lyase/homocysteine desulfhydrase"
                     /protein_id="HFF8011221.1"
                     /translation="MNKKTKLIHGGHTTDDYTGAVTTPIYQTSTYLQDDIGDLRQGYE
                     YSRTANPTRSSVESVIATLENGKHGFAFSSGVAAISAVVMLLDKGDHIILNSDVYGGT
                     YRALTKVFTRFGIEVDFVDTTHTDSIVQAIRPTTKMLFIETPSNPLLRVTDIKKSAEI
                     AKEHGLISVVDNTFMTPYYQNPLDLGIDIVLHSATKYLGGHSDVVAGLVATSDDKLAE
                     RLAFISNSTGGILGPQDSYLLVRGIKTLGLRMEQINRSVIEIIKMLQAHPAVQQVFHP
                     SIESHLNHDVHMAQADGHTGVIAFEVKNTESAKQLIKATSYYTLAESLGAVESLISVP
                     ALMTHASIPADIRAKEGITDGLVRISVGIEDTEDLVDDLKQALDTL"
     gene            17573..18598
                     /locus_tag="ACGEXS_000171"
     CDS             17573..18598
                     /locus_tag="ACGEXS_000171"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFF8011222.1"
                     /translation="MIEFRQVSKTFNKKKQKIDALKDVSFTVNRNDIFGVIGYSGAGK
                     STLVRLVNHLEAASNGQVIVDGHDITNYSDKMMRDIKKDIGMIFQHFNLLNSATVFKN
                     VAMPLILSKKSKTEIKQRVTEMLEFVGLSDKKDQFPDELSGGQKQRVAIARALVTNPK
                     ILLCDEATSALDPATTASILTLLKNVNQTFGITIMMITHEMRVIKDICNRVAVMEKGK
                     VVETGTVKEVFSHPKTTIAQNFVSTVIQTEPSTSLIRRLNDEQVGDFKDYKIFVEETQ
                     VTQPIINDLIQICGREVKILFSSMSEIQGNTVCYMWLRFNMDQQFEDTAINQYFKEKN
                     IQFEEVH"
     gene            18602..19261
                     /locus_tag="ACGEXS_000172"
     CDS             18602..19261
                     /locus_tag="ACGEXS_000172"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFF8011223.1"
                     /translation="MFGSDLDSAQLLQALYETLYMVSIALFLGAVIGIPLGVLLVITR
                     KQGIWPNIVIHQVLNPLINILRSLPFIILLIAIVPFTKLVVGTSIGTTAAIVPLTVYV
                     APYIARLVENSLLEVDEGIIEAAKAMGASPLQIIRYFLIPEALGSLVLAITTAIIGLI
                     GSTAMAGAVGGGGIGDLALVYGYQRFDTTVIIITVIVLVIIVQVIQTLGNVLARFIRR
                     H"
     gene            19298..20140
                     /gene="gmpC"
                     /locus_tag="ACGEXS_000173"
     CDS             19298..20140
                     /gene="gmpC"
                     /locus_tag="ACGEXS_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter
                     glycylmethionine-binding lipoprotein"
                     /protein_id="HFF8011224.1"
                     /translation="MKRLIGLVIVALVLLAACGGNNDKKVTIGVASNDTKAWEKVKEL
                     AKKDDIDVEIKHFSDYNLPNKALNDGDIDMNAFQHFAFLDQYKKAHKGTKISALSTTV
                     LAPLGIYSDKIKDVKKVKDGAKVVIPNDVSNQARALKLLEAAGLIKLKKDFGLAGTVK
                     DITSNPKHLKITAVDAQQTARALSDVDIAVINNGVATKAGKDPKNDPIFLEKSNSDAV
                     KPYINIVAVNDKDLDNKTYAKIVELYHSKEAQKALQEDVKDGEKPVNLSKDEIKAIET
                     SLAK"
     gene            20472..21476
                     /gene="aaa"
                     /locus_tag="ACGEXS_000174"
     CDS             20472..21476
                     /gene="aaa"
                     /locus_tag="ACGEXS_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin/adhesin Aaa"
                     /protein_id="HFF8011225.1"
                     /translation="MQKKVIAAIIGTSAISAVAATQANAATTHTVKPGESVWAISNKY
                     GISIAKLKSLNNLTSNLIFPNQVLKVSGSSNSTSNSSRPSTNSGGGSYYTVQAGDSLS
                     LIASKYGTTYQNIMRLNGLNNFFIYPGQKLKVSGTASSSNAASNSSRPSTNSGGGSYY
                     TVQAGDSLSLIASKYGTTYQKIMSLNGLNNFFIYPGQKLKVTGNASTNSGSATTTNRG
                     YNTPVFSHQNLYTWGQCTYHVFNRRAEIGKGISTYWWNANNWDNAAAADGYTIDNRPT
                     VGSIAQTDVGYYGHVMFVERVNNDGSILVSEMNYSAAPGILTYRTVPAYQVNNYRYIH
                     "
     gene            complement(21661..21930)
                     /locus_tag="ACGEXS_000175"
     CDS             complement(21661..21930)
                     /locus_tag="ACGEXS_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011226.1"
                     /translation="MAKRSKSQRLSSLLNVAGFIVDGYNGYKYHAKNKKLVYLSLGLS
                     TVGTVLDFYISIKSPRKFKKAVAVVTLITNGARLFTSIRKVKHEY"
     gene            22079..22474
                     /locus_tag="ACGEXS_000176"
     CDS             22079..22474
                     /locus_tag="ACGEXS_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFF8011227.1"
                     /translation="MIKCVCLVEETADKILLVQVRNREKYYFPGGKIEEGESQVHALL
                     REVKEELNLTLTMDEIEYVGTIVGPAYPQQDMLTELNGFRALTKIDWENVTINNEITD
                     IRWIDKDNDALIAPAVKVWIETYGGKHDK"
     gene            22464..22949
                     /locus_tag="ACGEXS_000177"
     CDS             22464..22949
                     /locus_tag="ACGEXS_000177"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8011228.1"
                     /translation="MTNNDTIMLRHYVPQDYSMLEAFQLSESDLKFVKTPEENITAAM
                     SDNERYPIVVMDGRQCVAFFTLHRGKGVAPFSDNQDAVFFRSFSVDQRYRNRGIGKVV
                     MEKLASFITSTFQDINEIVLTVNTDNPHAMALYRQQGYQYMGDSMFVGRPVHIMALTI
                     K"
     gene            complement(23118..23900)
                     /locus_tag="ACGEXS_000178"
     CDS             complement(23118..23900)
                     /locus_tag="ACGEXS_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFF8011229.1"
                     /translation="MNAVFILALLLLFLMIIFGGKKGFISYLTLFLNFAILIISIVLI
                     IFGVPIYVVTFFFCIIIGACNLFVLNSYNVKTQAAFISTIVTTILLITLIYLSVYFGH
                     LQGFSTEQQDETYVYSMNIGIDMVQFMVFTIILAVIAAVIDLTITISSPIYELHQVNP
                     TLTQYELFQSGMRVGREILATSANTIYLAFFGGQLALFIWFFKLKYSFGHIINSKIFA
                     QEFISIILGGIAVAISIPITAWITAFLIKRPSRQRKNDESIN"
     gene            complement(23897..25015)
                     /locus_tag="ACGEXS_000179"
     CDS             complement(23897..25015)
                     /locus_tag="ACGEXS_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFF8011230.1"
                     /translation="MFLKYRNLFTKPFNIILGLFCIAFIGAILFTFNNENFYNKPIGQ
                     IIDVKHVSSTPTKDAQNNRDIKYKNQLKVKILNGQFAGETTTINHQYVKSQADSEAFR
                     THEKVLLHISNKPSDAYIIEKKRDTLTVIITGLFLLTVLLVGRKVGLQSILSLILNSI
                     AILIAIYIHIQHSNINLFLLMTIAMICSTILTLLLVTGWHMRTLITIASTIIGTFLSI
                     GLTELIIYMTDGKGIKYETMNFLSLPPKDIFLASVLIGSLGAIMDVAITIASGMHEIL
                     QRTPHISMRRWALAGRNIGQDIMGTMTNILLFSYLSGALPMFLIFLKNANTVTYTISM
                     NWSLEIARALTGGIGIVLTIPITILFMEIFETLRRAKQ"
     gene            complement(25130..26014)
                     /locus_tag="ACGEXS_000180"
     CDS             complement(25130..26014)
                     /locus_tag="ACGEXS_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFF8011231.1"
                     /translation="MEIKQLRYFIEVAKREHISETALELNIAQSAISRQITLLEQELN
                     VSLFKKQGRNITLTSEGKLLFNEALRIIEHLDSTIEQFQSHGLTKNKSIYIGYDESDV
                     SHMLLPLIQTFHLQNDTHVIPSLLEHDTIINSVLNGNIDIGFTELTPEIRKHKQLHML
                     PLFEEHYHLYAPSDDPITMATHPPLIQFEHSHIYCLAPFAETVKKQLRKITKSDVYTI
                     SSQPLAQYLLRQKEGYIISSQNIHLPESKSWIDIKLDHTELKRTICAITKEPYTKSDI
                     GILLTLIQQLMTKTSTFH"
     gene            26195..30694
                     /gene="gltB"
                     /locus_tag="ACGEXS_000181"
     CDS             26195..30694
                     /gene="gltB"
                     /locus_tag="ACGEXS_000181"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFF8011232.1"
                     /translation="MHNEKLIKGLYDYREEHDACGIGFYANMDNKRSHDIIDKSLEML
                     RRLDHRGGVGADGITGDGAGIMTEIPFAFFKQHVTDFDIPGEGEYAVGLFFSKERILG
                     SEHEVVFKKYFEGEGLSILGYRNVPVNKDAIAKHVADTMPVIQQVFIDIRDIEDVEKR
                     LFLARKQLEFYSTQCDLELYFTSLSRKTIVYKGWLRSDQIKKLYTDLSDDLYQSKLGL
                     VHSRFSTNTFPSWKRAHPNRMLMHNGEINTIKGNVNWMRARQHKLIETLFGEDQHKVF
                     QIVDEDGSDSAIVDNALEFLSLAMEPEKAAMLLIPEPWLYNEANDANVRAFYEFYSYL
                     MEPWDGPTMISFCNGDKLGALTDRNGLRPGRYTITKDNFIVFSSEVGVVDVPESNVAF
                     KGQLNPGKLLLVDFKQNKVIENNDLKGAIAGELPYKAWIDNHKVDFDFENIQYQDSQW
                     KDETLFKLQRQFAYTKEEIHKYIQELVEGKKDPIGAMGYDAPIAVLNERPESLFNYFK
                     QLFAQVTNPPIDAYREKIVTSELSYLGGEGNLLAPDETVLDRIQLKRPVLNESHLAAI
                     DQEHFKLTYLSTVYEGDLEDALEALGREAVNAVKQGAQILVLDDSGLVDSNGFAMPML
                     LAISHVHQLLIKADLRMSTSLVAKSGETREVHHVACLLAYGANAIVPYLAQRTVEQLT
                     LTEGLQGTVVDNVKTYTDVLSEGVIKVMAKMGISTVQSYQGAQIFEAIGLSHDVIDRY
                     FTGTQSKLSGISIDQIDAENKARQQSDDNYLASGSTFQWRQQGQHHAFNPESIFLLQH
                     ACKENDYAQFKAYSEAVNKNRTDHIRHLLEFKACTPIDIDQVEPVSDIVKRFNTGAMS
                     YGSISAEAHETLAQAMNQLGGKSNSGEGGEDAKRYEVQVDGSNKVSAIKQVASGRFGV
                     TSDYLQHAKEIQIKVAQGAKPGEGGQLPGTKVYPWIAKTRGSTPGIGLISPPPHHDIY
                     SIEDLAQLIHDLKNANKDADIAVKLVSKTGVGTIASGVAKAFADKIVISGYDGGTGAS
                     PKTSIQHAGVPWEIGLAETHQTLKLNDLRSRVKLETDGKLLTGKDVAYACALGAEEFG
                     FATAPLVVLGCIMMRVCHKDTCPVGVATQNKDLRALYRGKAHHVVNFMHFIAQELREI
                     LASLGLKRVEDLVGRTDLLQRSSTLKANSKAASIDVEKLLCPFDGPNTKEIQQNHNLE
                     HGFDLTNLYEVTKPYIAEGRRYTGSFTVNNEQRDVGVITGSEISKQYGEAGLPENTIN
                     VYTNGHAGQSLAAYAPKGLMIHHTGDANDYVGKGLSGGTVIVKAPFEERQNEIIAGNV
                     SFYGATSGKAFINGSAGERFCIRNSGVDVVVEGIGDHGLEYMTGGHVINLGDVGKNFG
                     QGMSGGIAYVIPSDVEAFVENNQLDTLSFTKIKHQEEKAFIKQMLEEHVSHTNSTRAI
                     HVLKHFDRIEDVVVKVIPKDYQLMMQKIHLHKSLHDNEDEAMLAAFYDDSKTIDAKHK
                     PAVVY"
     gene            30712..32175
                     /locus_tag="ACGEXS_000182"
     CDS             30712..32175
                     /locus_tag="ACGEXS_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HFF8011233.1"
                     /translation="MGEFKGFMKYDKQYLGELSLVDRLKHHKAYQQRFTKEDASIQGA
                     RCMDCGTPFCQTGQQYGRETIGCPIGNYIPEWNDLVYHQDFKTAYERLSETNNFPDFT
                     GRVCPAPCESACVMKINRESIAIKGIERTIIDEAFENGWVAPKVPSRRRDEKVAIVGS
                     GPAGLAAAEELNLLGYQVTIYERARESGGLLMYGIPNMKLDKDVVRRRIKLMEEAGIT
                     FINGVEVGVDIDKATLESEYDAIILCTGAQKGRDLPLEGRMGDGIHFAMDYLTEQTQL
                     LNGEIDDITITAKDKNVIIIGAGDTGADCVATALRENCKSIVQFNKYTKLPEAITFTE
                     NASWPLAMPVFKMDYAHQEYEAKFGKEPRAYGVQTMRYDVDDKGHIRGLYTQILEQGE
                     NGMVMKEGPERFWPADLVLLSIGFEGTEPTVPNAFNIKTDRNRIVADDTNYQTNNEKV
                     FAAGDARRGQSLVVWAIKEGRGVAKAVDQYLASKVCV"
     gene            32444..32536
                     /locus_tag="ACGEXS_000183"
     tRNA            32444..32536
                     /locus_tag="ACGEXS_000183"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32480..32482,aa:Ser,seq:tga)
     gene            33007..34434
                     /gene="treP"
                     /locus_tag="ACGEXS_000184"
     CDS             33007..34434
                     /gene="treP"
                     /locus_tag="ACGEXS_000184"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFF8011234.1"
                     /translation="MAVKREDVKAIVTAIGGKENLEAATHCVTRLCLVLKDESKVDKD
                     ALSNNALVKGQFKADHQYQIVIGPGTVDEVYKQFIDETGAQEASKDEAKQAAAQKGNP
                     VQRLIKLLGDIFIPILPAIVTAGLLMGINNLLTMKGLFGPKALIEMYPQIADISNIIN
                     VIASTAFIFLPALIGWSSMRVFGGSPILGIVLGLILMHPQLVSQYDLAKGNIPTWNLF
                     GLEIKQLNYQGQVLPVLIAAYVLAKIEKGLNKVVHDSIKMLVVGPVALLVTGFLAFII
                     IGPVALLIGTGITSGVTFIFQHAGWLGGAIYGLLYAPLVITGLHHMFLAVDFQLMGSS
                     LGGTYLWPIVAISNICQGSAAFGAWFVYKRRKMVKEEGLALTSCISGMLGVTEPAMFG
                     VNLPLKYPFIAAISTSCVLGAIVGMNNVLGKVGVGGVPAFISIQKEFWPVYLIVTAIA
                     IVVPCILTIVMSHFSKQKAKEIVED"
     gene            34498..36138
                     /gene="treC"
                     /locus_tag="ACGEXS_000185"
     CDS             34498..36138
                     /gene="treC"
                     /locus_tag="ACGEXS_000185"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFF8011235.1"
                     /translation="MSKEIDWRKSVVYQIYPKSFNDTTGNGIGDINGIIEKLDYIKLL
                     GVDYIWLTPVYESPMNDNGYDISNYLEINEDFGTMDDFEKLIKVAHQKDLKVMLDIVI
                     NHTSTEHEWFKEARKSKDNPYRDYYFFRSSEDGPPTNWHSKFGGNAWKYDSETDEYYL
                     HLFDVSQADLNWDNPEVRQSLYRIVNHWIDFGVDGFRFDVINLISKGEFKDSDKIGKE
                     FYTDGPRVHEFLHELNRQTFGNTDMMTIGEMSSTTIENCIKYTQPERQELNSVFNFHH
                     LKVDYVDGEKWTNAKLDFHKLKEILMQWQRGIYDGGGWNAIFWCNHDQPRVVSRFGDD
                     TSEEMRIQSAKMLAIALHMLQGTPYIYQGEEIGMTDPHFTSIAQYRDVESINAYHQLL
                     SEGHAEADVLAILGQKSRDNSRTPMQWSDDVNAGFTAGKPWIDISENYHQVNVRQALQ
                     NKESIFYTYQKLIQLRHTHDIITYGDIVPRFMDHDHLFVYERHYKNQQWLVIANFSAS
                     AVDLPEGLAREGCVVIQTGTVENNTISGFGAIVIETNA"
     gene            36163..36891
                     /gene="treR"
                     /locus_tag="ACGEXS_000186"
     CDS             36163..36891
                     /gene="treR"
                     /locus_tag="ACGEXS_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFF8011236.1"
                     /translation="MAKQKKFMKIYEALKEDILNGQIQYGEQIPSEHDLVQLYQSSRE
                     TVRKALDLLALDGMIQKIHGKGSLVIYQEVTEFPFSELVSFKEMQEEMGVAYLTEVVV
                     NEVVEAHEVPEVQHALNINSSESLIHIVRTRRLNQHVKIVDEDYFLKSIVSDIGNDVA
                     SDSIYDYLEKVLNLNISYSSKSITFEPFDEQAYQLFGDVSVAYSATVRSIVYLENTMP
                     FQYNISKHLANEFKFNDFSRRRIK"
     gene            36953..37222
                     /gene="ffs"
                     /locus_tag="ACGEXS_000187"
     ncRNA           36953..37222
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACGEXS_000187"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            37291..37392
                     /locus_tag="ACGEXS_000188"
     CDS             37291..37392
                     /locus_tag="ACGEXS_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011237.1"
                     /translation="MKTIQRIIRGTCLWEVAFLYVKFDSSELDVQFE"
     gene            37530..38054
                     /locus_tag="ACGEXS_000189"
     CDS             37530..38054
                     /locus_tag="ACGEXS_000189"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8011238.1"
                     /translation="MQIYLSTLTELDYDKSLNSIEESFDDNPETSWQARAKVKHLRKS
                     PCYNFELEVIAKNENNDVVGHVLLIEVEINSDDKTYYGLAIASLSVHPELRGQKLGRG
                     LVQAVEERAKAQEYSTVVVDHCFDYFEKLGYQNAAEHDIKLESGDAPLLVKYLWDNLT
                     DAPHGIVKFPEHFY"
     gene            38123..39820
                     /gene="dnaX"
                     /locus_tag="ACGEXS_000190"
     CDS             38123..39820
                     /gene="dnaX"
                     /locus_tag="ACGEXS_000190"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFF8011239.1"
                     /translation="MNYQALYRMYRPQSFEDVVGQEHVTKTLRNAISKEKQSHAYIFS
                     GPRGTGKTSIAKVFAKAINCLNSTDGEPCNECHICKGITQGTNSDVIEIDAASNNGVD
                     EIRNIRDKVKYAPSESKYKVYIIDEVHMLTTGAFNALLKTLEEPPAHAIFILATTEPH
                     KIPPTIISRAQRFDFKAISLDQIVERLKFVADAQQIECEDEALAFIAKASEGGMRDAL
                     SIMDQAIAFGDGTLTLQDALNVTGSVHDEALDHLFDDIVQGDVQASFKKYHQFITEGK
                     EVNRLINDMIYFVRDTIMNKTSEKDTEYRALMNLELDMLYQMIDLINDTLVSIRFSVN
                     QNVHFEVLLVKLAEQIKGQPQVIANVAEPAQIASSPNTDVLLQRMEQLEQELKTLKAQ
                     GVSVAPVQKSSKKPARGIQKSKNAFSMQQIAKVLDKANKADIKLLKDHWQEVIDHAKN
                     NDKKSLVSLLQNSEPVAASEDHVLVKFEEEIHCEIVNKDDEKRSSIESVVCNIVNKNV
                     KVVGVPSDQWQRVRTEYLQNRKNEGDDMPKQQAQQTDIAQKAKDLFGEETVHVIDEE"
     gene            39910..40227
                     /locus_tag="ACGEXS_000191"
     CDS             39910..40227
                     /locus_tag="ACGEXS_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002479279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFF8011240.1"
                     /translation="MRGGGNMQQMMKQMQKMQKKMAQEQEKLKEERIVGTAGGGMVAV
                     TVTGHKEVVDVEIKEEAVDPDDIEMLQDLVLAATNEAMNKADELTQERLGKHTQGLNI
                     PGM"
     gene            40234..40830
                     /gene="recR"
                     /locus_tag="ACGEXS_000192"
     CDS             40234..40830
                     /gene="recR"
                     /locus_tag="ACGEXS_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFF8011241.1"
                     /translation="MHYPEPISKLIDSFMKLPGIGPKTAQRLAFHTLDMKEDDVVQFA
                     KALVDVKRELTYCSVCGHITENDPCYICEDKQRDRSVICVVEDDKDVIAMEKMREYKG
                     LYHVLHGSISPMDGIGPEDINIPSLIERLKNDEVSELILAMNPNLEGESTAMYISRLV
                     KPIGIKVTRLAQGLSVGGDLEYADEVTLSKAIAGRTEM"
     gene            41035..41133
                     /locus_tag="ACGEXS_000193"
     CDS             41035..41133
                     /locus_tag="ACGEXS_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011242.1"
                     /translation="MNWIIELDLWLWCVGAINLINAVNDYANEILL"
BASE COUNT        14072 a         5972 c         8519 g        13016 t
ORIGIN      
        1 aaaatgaatt acatgtatta aattgaataa gttagttttg tttgataaca taaaagttga
       61 ataatcacta ttttaataag ttgtatgaaa tattcaaatg tttataaaat taaaacagag
      121 agatgtgaaa tgatggagta tataaaaaaa attgctttgt acatgagtgt attactttta
      181 atcattttta ttgggggatg tggaaatatg aaagatgaac agaaaaaaga ggaacaaacg
      241 aataaaacag attcaaaaga agaacaaatc aaaaagagtt ttgcgaaaac gttagatatg
      301 tatccaatta agaatctcga ggatttatac gacaaagaag gatatcgaga tggtgagttt
      361 aaaaaaggcg ataaaggtac gtggactata cttacaggtt tttcaaaaag taacaaacca
      421 ggagtattag atgatgaagg catggtgtta tatcttaata gaaataccaa aaaggcaaca
      481 ggttattatt ttgtaaataa agtttatgat gatattagca aaaatcataa tgagaaaaaa
      541 tatcgtgttg aactaaaaaa taataagatt gttcttttgg ataatgtaga agacaaaaaa
      601 cttaaacaaa aaattgaaaa ttttaaattt tttagtcagt atgctgattt taaagattta
      661 aaaaattatc aagatggaaa tataacaact aatgaaaacg taccgagcta tgaagcacaa
      721 tataaaatga acaatagtga taaaaatgta aaaaaactta gagaaattta tccaattaca
      781 actaataact ctccaaatct aaaattatat atagatggtg atataaaagg aagctcagta
      841 ggatataaaa aaatagaata taaattttca aaagataaag gtcaagagac aacattaaga
      901 gattatttga attttggacc gtctgaaggt gagaatgttg agtaggaagt ataaaataga
      961 tttaaaagca attaatcaaa atactgagtc tacaagtgct atttcgaaag cgtcatacga
     1021 agttgagaat gctaataata atggtctatc aaaaagagac gtgattaatc aatttaatga
     1081 tttgaaaaaa atgaaaaaat tcccttctaa tcttgaatat gttgatagtt acactgattc
     1141 tcttactgga gtaacaactt ctgctttttt aaataaagat acaggcaaag taactctcgg
     1201 gatgactggg actaatttac aagacgaagc ctttaaaaag ttaaaagaag gtgaattttc
     1261 aagacaaaat gttaccaatg ctttggaaac agttaaagat ggatatgcag atcttaaaat
     1321 attatattct cctgcatctg atcaaaacta tagatatgcg aatacacaag aatttataaa
     1381 taaaataaaa agtaagtatg acattgattt tattactgga cattcactag gtggaagaga
     1441 tgcggtagtt ctaggaatga gtaatggtat tccgaacatt gtggtttata atccagctcc
     1501 tatttctata actagtttga atcctaattc cccagatgga aaacgtttat tagaattata
     1561 taaaaattat aaaggtaata ttactaggtt tgttgcagaa aatgatgcat tgacagaaaa
     1621 tctgaagaaa tataagcatt atgttttttt cggtaatgat aaagtcttta aaaatggtaa
     1681 aggtcatgaa atggaaggct ttctgaccga agaagaacaa aaagctataa aaaaagaact
     1741 taaaaaacta caaggttatg cagaagaaaa taataagtca tttgtaaaga attcaaataa
     1801 tgctatctct aaattagcta gtatagaatt acttagagca aatatgatga ctacaaatgg
     1861 aggaggatta tcttcttcgc agcaaaaagt tttagaaagt ttaacagctt taacaattgc
     1921 gcagtcattc agtcaactga tagacgatga aattaatcaa atcaaaaaaa tgtataatga
     1981 aaagaaaaag aaatttggaa aaaattggga agacgctcaa aaagctggaa aagctgtagg
     2041 tgaagatttg agtgtaaatg gagttctcaa tgctttagat gaaggtcaag tgaatgaaag
     2101 tagtatggta agagaacctg aacaaatgat atctgcaaaa gaaagacaac tttcaacgat
     2161 agggtcttct gtatcaaatt atattatgag agttagactc agtattaatg aaatcgttga
     2221 taaagatcaa gtgcttgcat cacaaatagg tggattacta tgatgtttaa tcaaattaat
     2281 aataaaaatg aattagaaga atcatatgaa tctgagaaaa aacgtataga gaatgaactg
     2341 caaaatttaa atgaacttag gcatagaact cgaaaagaaa atgaacgtag ttatgatgtt
     2401 tttcaatatt tgaagcacga aatgaattat agtgaagatg cccaaaggaa aatgacgaga
     2461 aatatagaag cgtatgagca agaaatcaat gagataatta gaaagcaaga atggaaatta
     2521 gaagaatata aagaagactt aaaaaagtct tatgaaaagc agttagataa gctaagtgac
     2581 tgataatttg gaggagatta aatgactcta atagaaccag atatgacctt aagaatgcca
     2641 gatataagta ctacagtaga aacacttaat ctcatatcta aaatggaagc gcaaaaagaa
     2701 aatattcgca cagttattgc acctgaacat aagcataaat acaaagatat tgaaaacgga
     2761 ttaaaaggtg aagaaaaagt attaattgaa caaatggcgc aacattgcga agcttttaaa
     2821 gctaatttta aaggtgcagc tcaaggagat tgggttaaaa gtgccatgtc tgagatagac
     2881 agcattaagg atgacctgaa aaaaattaat agctaaacta catatcatac tactaaataa
     2941 agcgaccaat gttcagtata ttcacaactg acactaggcc gcttcttttt aatttatatt
     3001 attcccatgt aaagtaattc gaaccgaatc gaaatatcac actctcaatt gtaaaaaaac
     3061 aacttaaatt actgtcccgc attaaacatg accacattcg taacagcaac aataaaaatc
     3121 ctcaagtact aaaagctatg atcactttat caaaatcaac gcaagttaac ctctcccgaa
     3181 ctacccgtta ctcatcacct tcaccttaca aaccataaag cgctgcagtt aagtattgaa
     3241 tggcatagcc aattgaaatg aaaatagcaa tcgttataat agcactgcca atggtatata
     3301 aagtatgttt cgtagataat ccttcagtct ttgaaccaaa aacactgagt agaataaatg
     3361 caataccagt aacatataaa actaaaatga caaataacat gtttatacac ctcgattctc
     3421 tttcgtgaga tatatctttg tctccattat aaagtaaagc gctcaataat ttaattaagt
     3481 atactatttt tttattatcg aagattgcga aagaggaaac gctcctatat aatgtaaaac
     3541 ggaattattc ctatttacaa ataaattgaa gtgagggtat atatatggct aaaattccag
     3601 ttacggtatt aagtggttat ttaggctcgg ggaagacaac gttgttaaat catattttac
     3661 aaaatcgaga aggtcgacgt atcgcggtaa ttgtaaatga tatgagtgaa gtaaatatcg
     3721 ataaagatct tgtcgcagat ggtgggggac tatcgcgtac agatgaaaaa ttagtcgaac
     3781 tttctaatgg ttgtatctgt tgtacactta gagacgattt attaaaagaa gttgagcgtt
     3841 tagtgaaaaa aggtggcatc gatcaaattg ttattgagtc aacagggatt tcagagccag
     3901 tacctgttgc acaaactttc tcatatattg atgatgaact tggcattgat cttacagcga
     3961 tttgccgttt agatacaatg gttacagttg tggatgctaa ccgcttcgta catgacatca
     4021 actcagaaga tttattgatg gatcgtgatc aaagcgttga cgaaacagat gagcgttcga
     4081 ttgctgattt attaattgac caagttgaat tttgtgatgt attgattatt aataaaattg
     4141 atttaattag tgaagaagaa ctagcgaagt tagaaaaaat gttaagcgca ttgcaaccga
     4201 ctgctaaaat tattaagaca acaaattctg aagtagattt aaaagaagtc ttgaatacgc
     4261 agcgttttga ttttgaaaaa gcgagcgagt cagcaggatg gatcaaagaa cttgagtctg
     4321 gtgggcatgc atcgcataca cctgaaacag aagaatatgg tatatcatcg tttgtatata
     4381 aacgtcgtct acctttccat gctaaaaggt tcaatgattg gttagaaagc atgccaaata
     4441 atgtcgttcg atcaaaaggt atcgtatggc tagcacaata caatcacgta gcatgtttat
     4501 tatctcaagc agggtcatct tgcaatattc atccagttac atattgggtg gctagtatgt
     4561 ctgaagcgca acaaacacaa atattagcag aacgtcaaga tgtcgcagct gaatgggatc
     4621 cagaatatgg cgatcgtcat acacaatttg tcattattgg tacagaatta gatgaagaaa
     4681 aattaacaaa agaactcgac gcatgcttag tcaatgcgca agaaattgat gcagattggc
     4741 aacaatttga agatccatat caatggcaaa ttagaccagc acgataagtt gaatgaagta
     4801 taatcatttt tgaattgtgg cttaattgtt tgcaatttat gacaattaat aaaaagttaa
     4861 ggtgttctta ggatcttttt gttttcaatc tatctaaagt gtgatagttt tgataaagca
     4921 gaaatttgca ttttatccat agggctagga catgtatgtg tcttagtcct ttttatattt
     4981 acgttgatga aaaatggcaa aaccatcgta ccaactgtta attgaaggta caatcattgt
     5041 ttaacagtca taaaaatgag tgaagtaaat gcagctatca atgtactatt aacacttacg
     5101 gtcacacgag tgtttcagtc taaacgtgca acgcaattcc gcacgtggcc aatatatctt
     5161 gcgctagatc atgaaaatca ttaagatttc gcatgccaaa tcatgaatgt ctcagaactc
     5221 gagtatcaaa cataaaacac gccacaaaaa tataacgcgt cagactacaa agatcacgaa
     5281 tattccgtat gtcccactaa gaacgtccta tacaaaaaac accaagacca tttatttgca
     5341 acaaactaac aagactcacc tcacatcaat aaatcaacac aaagcaaagc caccatccct
     5401 attggtatag tggcctgaga attttaagta ttcaattcgc ttaaattatt ttgcgaaaat
     5461 gtcgataatt gctttgatga ttttaatgat agtacctaca atagccatcg ttttgtcctc
     5521 ctgtatgttg atttaacata gtgatgagtt gttgatcgtt aatgtttgag attagttgtt
     5581 acctaaaaat ttaccaagta aatctttaaa gaatttgaat aattttgcta cgaattccat
     5641 gtgaatggcc cccttcaaat aagatgttca tatctatgag atttttataa cttacttacc
     5701 agtgaatttc tcaattaatc ctttaatgaa tttaatgatt cctgcaatga tacccatgtg
     5761 aaagacctcc tttgtttgtt atgaaatctt atttaccagt gaattgttcg attaagcttt
     5821 tgataacttt aatgatgcca gcgatgatac ccattaagat tacctccttt gcttatgagt
     5881 taacttcatt gtacatagtt atcttgtgcg taattgattt ttttcctaac ttgaattgtt
     5941 atgatcataa ctgcgaattt tttattttaa aaggacggga aatactgcct aactgtacag
     6001 gtgcatttac aaaatcttta caatcattgg tttaatagag gttaagctca tgaatttgac
     6061 atgaagcaga aatttaatta tattttatgt tataagttta aaacgaataa cacgttaggt
     6121 ctcgtctagg caaagcatat ttatgaagat gaattgaaca gacagaagta acattttata
     6181 tacatatgtg catgggagca cgcttaaaga gaacactgca acaaggtata tcaacgtacc
     6241 gtgatgcagt gtctagttaa actgaaacct gcaatagtaa attttctgcc attatgtaca
     6301 gaatctacta ttgtaggttt ttatttttat acataagcaa ttatgcgaga gattagaaat
     6361 aaggaggtta tgcagtgtta agttttcaat tgctattttc actgtttgtt attgcgctta
     6421 tcattgcatt gataagtggc ttgttgtttt tagcaccagt tatgccaatg agatatatta
     6481 aattacattt atacatacta gtcatgccag tattatttgc agtcattggc tttttcggta
     6541 ttcatggtca acatgtctta ggtccattta aaatagatcg tttatcttgg ttattagctg
     6601 gctttgtaat ggcgcttggc tttattattc aaaagttttc aatgcgatat ttactaggtg
     6661 atcatcatta tagacattac tttccattgt tcactgcgat tacgtcgttt gcatctttag
     6721 catggatgtc tgaagactta agactgatgg cactctgctg gggtattaca ttattatgtt
     6781 taacattgct gatgaacgtt aatcgttttt ggaaagtgcc acgtgagtct gcgaaattat
     6841 caagcatgac atttttatgt ggttggcttg cattcgttgg agcaattgta actatttata
     6901 ttgcgactgg cgagtggcgg gtaccacaac atatagttca tccgacatgg tcattgttga
     6961 cgaatgtact acttgtatta gctgtcatga taccggcagc acaatttcct tttcatcgat
     7021 ggttgattga atctgtaacg gcaccaacgc cagtatcggc aattatgcat gcaggaattg
     7081 tgaatgcagg tggtgttatt ctaactcgtt ttgcgccgat atttgataat ggatttgcgt
     7141 tatcattatt acttatcctt tctagtattt ctgtattgtt aggatcgggt attagcttag
     7201 ttcaagttga ctataaacgc caattagtgg gctctacgat gagtcaaatg ggctttatgt
     7261 tagttcaatg tgcattgggt gtatattcag cagcgattat tcatttaata ttgcacggta
     7321 tttttaaagc aacattattt ttacaatcag gttctatcgt gaagcgattc aatattccaa
     7381 aacaagcatc tgctaaagac gcttatggct ggattgtcat gggacgtgta ttagctatta
     7441 tcgtggcatt tctattttgg atgagtagtg acagaagtgc atatgaagtg ttaagtgcac
     7501 tcattctagg atggtcatta cttgtatctt ggaatcaaat ggtagccttt agtaaaggac
     7561 gcatggcacg tcttgttggt atgattttga ttgcaatcgt gacatttatc tacgtcatca
     7621 cacataatta tttttacgct gtattacaaa atataacaac acatgcgaca acgccgccta
     7681 cagtgagtgt catcattagt gttgttattt taatctttgg cagtttatta agtatttggg
     7741 tggcgcggca tcgatactct aaggcttttg cggtattgta cgtgtggtta gttaatctag
     7801 gtgaagcacg ctcgaaagcg atagaaagtc atccgaatta tttgaagaag tatttatagg
     7861 aggtgaaagg tatgacaaca cagttaaata tcaattcagt cattgaaaat gcgaaacgtg
     7921 ttattacacc attatcacca atttcgattt ttgcagcacg aaatccatgg gaaggattag
     7981 aagcggatac gtttgaagat gtcgcaaaat ggttacgtga tgttcgagat gtggatattt
     8041 tcccaaataa agcattaata gaaagcgctg tggcacgtgg tgaattagat gaaagtgtct
     8101 ttaatcaact tgttactgat atgttacttg aacatcacta caatatcccg caacactaca
     8161 tcaatcttta tattgataac attaaaacat taaaagacgt acctgcatca tatatgaatc
     8221 attcaaatgt tgatgttgtt gctgatctac tattagaaaa atcaaaacgt gatatggctg
     8281 aatcatatca tcactatgat gtacgtccga tgagtgatgc aataatagat gaacaaggtg
     8341 agccacttag cgaacaagtg aatcgtcaaa tgattaaatg gacgaaactt tatatcgatc
     8401 aatttctatc gagttggaca atgccgaagc gtgagcaaag tttttaccat gcatggttgc
     8461 atttagcgca acatgaccat agttttacta aagcacagcg ccaagtgatt aaaggcttac
     8521 ccaatgatcc tgaaatgacg atagagtcag tattaactca tttttcaata gatcaggaag
     8581 actaccaagc ttatgttgaa ggacatcttt tggcgttacc gggttgggca ggtatgttgt
     8641 attaccgttc acaacagcat cactttgaac aacatttgtt aacggattat ttggcaattc
     8701 ggttagttgt cgaacaattg ctagttggtg atgagtttaa gtcagtcgct aaagattgtg
     8761 aaagtagatc ggaaaattgg tttaagcaaa ctgttgcatc atggtgttac tacagtgata
     8821 tgcctagcga tgtattacta caacatgacg tcaatgaaat tcaaacgttt attcattttg
     8881 cagcaactat gaataaaaat gtatttaaaa atttatggct aattgcctgg gaaatgacat
     8941 acgaatctca gttaaaacaa aaaattaaag caggtcatga aagtgtggcg ggcgcattag
     9001 atgtaaacca agtaaatgtc tcagaaaatg ataacgctaa tcagccacat tcagtattgt
     9061 taaatgacac acaagcagtt gatgaaaata atagcgagct aaatcagatg ggcacatcaa
     9121 cgaaagcgca aattgcattt tgtatagatg ttcgttcaga accatttcgt agacatatcg
     9181 aagcagcagg gccctttgaa acgattggta ttgcaggctt ctttggatta cctattcaaa
     9241 aagatgccgt agacgaacaa ttcaaacatg attcattacc tgtcatggta ccgccggcat
     9301 atcgcattaa agaatttgca gaccgctacg atatgaatgt ttatcgacaa cagcaacaga
     9361 caatgtcatc gatgttttac acatttaaat tgatgaaaaa taatgtcatg cctagtctgt
     9421 tattgcctga attaagtggg ccatttttaa gcttaagtac cattgtcaat tcgattatgc
     9481 ctagaaaaag tcgcgcgtct ttacaaaaaa taaaacaaaa gtggttgaaa aagcctgaaa
     9541 caaagttgac gattgatcgt gagtttgacc gaacatcaga cttacctgtt ggatttactg
     9601 agcaagagca aattgatttc gcgttacaag cgttgaaatt gatggattta accgaagcat
     9661 ttgcgccgtt cgttgtgtta gcaggtcatg ctagtcattc tcacaataat ccacatcatg
     9721 catcacttga atgtggggct tgtggtggcg catcaagcgg ttttaatgct aagttattag
     9781 cgatgatatg taatcgtcca aatgtcagac aaggattaaa acaatcaggt gtgtatattc
     9841 cagagacaac tgtttttgcg gtagcagaac atcatacgtc tactgatacg ttggcatggg
     9901 tatatgtgcc agacacatta tcttctattg ctttagatgc atatgaatca ttgaatgacg
     9961 cgatgccgat gatttctgaa cacgcgaatc gcgaacgttt ggacaaactg ccaacgattg
    10021 gtcgtgtgaa tcatccagtg gaagaagcgc agcggtttgc gagtgattgg agtgaggtac
    10081 gtccagaatg gggattggct aaaaatgcat catttataat tggacgacgc caattaacaa
    10141 aaggcattga tttagaaggg cggacatttt tacacaatta tgattggcgt aaagataaag
    10201 atggcacatt attaaatacc atcatttctg gtccggcact tgtggcacaa tggattaatt
    10261 tacaatatta tgcgtcgaca gttgcgccgc atttttacgg aagtgggaat aaagcgacac
    10321 aaaccgtcac gtcaggtgtt ggtgtcatgc aaggtaatgc gagtgatctg atgtatggct
    10381 tatcatggca atctgttatg gctgctgatc ggacgatgta tcattcgcca attcgtttac
    10441 ttgtcgttat tcaggcaccc gactatgttg tagcaagact actcgcgaat aatgagcatt
    10501 tcgctaggaa ggtgtctaat cattggctgc gtttaatgag cgttaatgag gaagggcgtt
    10561 ttaaaagttg gatttaacga tgatatgttg cgatgctaag taactaatat atcatcatat
    10621 ctacaatgtt ctagcttctt aaaatatact aaaaatgata aacttaatgt aatagttgat
    10681 gtcgtgataa cgatacaaag atgaagtaat caattttaat attattggag ttagtgatat
    10741 gaaaagaacg aaaggtgaaa tcgaagctga aatcagtaaa gccattacgc aatgggaaaa
    10801 agatttcctt ggcagaggtt ccttgtcagt taaatcagac attttaagag atatggtgat
    10861 tattagttta caaggtatct taacgccagc tgaatatcgt gtatgtagta cgaatgaagg
    10921 attattaaat attaaacgaa cacgttctga attagttgaa tccggtgagc aagatttgaa
    10981 tgatatcatt tttaaaatta caggtatcaa agtgatgagc ttccatagtg atttaagtac
    11041 agttacaggt gaacgtatta tcgtattcaa acttgaggat aatttggaaa agcatattta
    11101 agaaggttag gtatgcttct gctggcttat taaagctata gaagtatgct tggttaaaca
    11161 gcgcaacgcc atatatttgg gtgtgctgtt tttctattac atgatgtgtt tattacgtgg
    11221 atgaatgcgt gggtctgaat atagcccgtg aaaggcgtat gtcataaaat ttgcgaatgg
    11281 tgacaagtcg acgcaagcat atttgttgcg agacatgccc actatccttt tacataaaga
    11341 gtatatgtgt gacgtaggca tataatcgat aaagtattcc taaaaaatta ggtataatga
    11401 ccattgttgc aataagtttt tcggccttga aaccgataac gcatagtata ataggaataa
    11461 aatataatac aaatatccag taaatcaaat ttgaaaatga tggatgaata ggggtaaaga
    11521 atctaactaa tgtaatgatt aacgctatat agacaaaaat aactctattg atacgcctta
    11581 ccccctctgt ataataaata taaatcgtac taaattgaaa atagcaaaat attgttattt
    11641 caattataca atgttttatt tgcaatatac ataacaatat gaactttata attttgatat
    11701 tgtcggtaaa gtgaaatttg tggcacattg atattatgga taacatttgt aaaatgaaaa
    11761 tgatgatacg aggagattgt aagatgatag ataaaaaatt aacatcaccg aaaatgacag
    11821 tgcctttatt tttaatcgcg ctgattgtat ttataggtat gttttacagt gtagtgacaa
    11881 atcaagaatg gcttaaaaat atagatatgg gatcattaac atggtttaca gattatttcg
    11941 gtgagccaca acgtcagtat gttaacaatt tgtttaatta ctatatgacg tttagtgcgg
    12001 aaattggaga tgtcaaaggt gtcgtgttga tttccattat cgtcacaatc atactgttta
    12061 ttaaacagag gcatttagcg gtttggtttg tgacatattt ggtttcaggt gtcatcatga
    12121 acaaattaat taaagatact gtattacgtc caagaccata taatcattta gccgttgata
    12181 caggcttttc atttccgagt ggacattcca acgccagcac attattatat ttcgccttaa
    12241 tgatcattat tatttcactt gctgctaaga caataacaaa agtgttgagt gcgttggtta
    12301 tgggaatatt atggcttagc atattatttt gtcgccttta ttttcatgca cattactttt
    12361 cagatgtcat tggcggcacg tcactagcaa tcatttgggt agcgttattc ttaatggtat
    12421 acccatactt tattaatcat cgacgacaac gcgtttagca aacatcttta gacatagtgg
    12481 aggtatatag aaaaatgaga attaaaacac cgagtccatc gtatttaaaa ggcacaaatg
    12541 gacatgcgat attattatta cattcattta caggtacaaa tcgggatgtg aagcatcttg
    12601 cagctgagtt aaatgaccaa ggatttagtt gttatgcacc gaattatcca ggtcatggtt
    12661 tattgttgaa agatttcatg acatataatg tagatgattg gtgggaagaa gttgagaaag
    12721 cttaccaatt tttagtcaat gaaggttatg aatctatcag tgcaacgggt gtgtctttag
    12781 gtggattaat gacattaaaa ttggcgcaac actatccttt gaaacgtatc gctgtcatgt
    12841 cagcaccaaa ggaaaagagt gacgatggct taatagaaca tttagtttat tatagtcaac
    12901 gcatgtcgaa tattttaaat ttagatcagc aagcatcgag tgcgcaatta gcagcaattg
    12961 atgattatga aggtgaaatt acgaagtttc aacattttat tgatgatatc atgacaaatt
    13021 taaatgttat taaaatgcca gctaatatat tatttggtgg taaagatgcg ccatcctatg
    13081 aaacaagtgc acattttatt tatgaacatt taggatcagt agacaaagaa ttaaatggtc
    13141 tgaaggattc gcatcattta atgacgcatg gagaaggcag agatatttta gaagaaaatg
    13201 ttattcgctt tttcaatgct ttaacataat tgaagatttt aaaaattaac acttgagtga
    13261 cgatgttatt atatttgtca tttgagtgtt ttttgtggcg tgatagtata gggaacttag
    13321 ctaaagttaa tttgtagtgt gatgtcgtgg aaatattatg gagttatacc agaagttgat
    13381 ttgtattgta atgtcgtgga aattttatgg gcattgcacc tgaagttgtt atgtggcgag
    13441 atgttgggga tatagatggt gttgtatgtg gcgagatatt ggcgcacgta tacagcgttg
    13501 aaccagaaaa tttaatgtag cgtaatgaca acacattaca agagttgatt atattatgat
    13561 aaatccaggt gctaccgtat taatgaaaca attttgatgc aggtgtattg catgttattt
    13621 acatacatgt tagagtgtta cttaaacacg tagacgcttt aacgctttaa aggagatgtg
    13681 aatgatgaaa agacaacaat cacaatggaa gtcatcaact ggatttattt tagctagtgc
    13741 gggttctgca atcggtcttg gtgccatgtg gaaattccca tatatggcag ggatttatgg
    13801 cggcggtgcc tttctagcta tgttcttaat attcaccatt tttgttgggt tgccattact
    13861 cattatggaa ttcactgttg ggaaaatggg acggacatat acaacacaaa tatatagtaa
    13921 attaactggt aaaaaatggc tcaatatcat tggctggaac ggtaatttgg cagtgtttat
    13981 tttatttggc ttctatagtg ttatcggtgg ttggattgtc atttacatcg gacaagtatt
    14041 atggcaatta gttatatttc aacgcatcaa tcatctccaa gaaatgaatt ttgaagcggt
    14101 aatatcaaat ccttggttaa ccgttctagg gcaaggtata ttcatattcg ctacgatgat
    14161 tattgtcatg ttaggtgttg aaaaaggatt agaaaaggca tcgaaagtta tgatgccatt
    14221 gctgtttgtc tttttaatcg tcattgtgat taagtcttta acattagatg gcgccttaga
    14281 aggtgtgaaa tttattttac aaccaagagt atcagagatt actgctgatg gcatcttgtt
    14341 tgcgctaggt caatcattct ttacgttatc attaggaact acaggtatga ttacttatgc
    14401 gagttatgcc tctaaagaca tgacgattaa gtcatcagct atttctatcg ttgttatgaa
    14461 tatctttgta tctgtattgg caggtctagc tatatttccg gctttacata gttttggcta
    14521 tgaaccacaa gaagggcctg gattattatt taaagtactg ccaatggtct ttagtcaaat
    14581 gcatctaggc acattattct atttgggatt cttagtgctg ttcttatttg cggctttaac
    14641 gtcatctatt tctttattag aattaaatgt ttctaacttc acgaagaatg acaatacaaa
    14701 acgtaaaaaa gtcgcagtga tcggtagtat tttagtattt atcattagta ttccagcaac
    14761 cttatctttt ggtatcttaa aagatgtaag attcggtgcg ggaacgattt ttgataatat
    14821 ggatttcatc gtttcgaatg tattgatgcc attaggcgca ttaggtacta cgcttgtcgt
    14881 aggacaatta ttagataaaa aattattaca acaatatttt ggtaaagatc gatttagatt
    14941 attcagtggt tggtattact taattaagta tgcgatgcct gtcgttatta ttttagtctt
    15001 tatcgtgcaa ttatttagtt aatatataga agcatctgac acacaagtat ttgtgttgct
    15061 caggtgtttt tttgtttgga attgaaaatt tagtactggt tctgaattaa tcgaaactta
    15121 gataattgca aaataacaaa atgtcgttta taattcttat ctgaaaagta agaattaaaa
    15181 ttgagacatg aagaaaggtt tcattcattt gatcggcatg tcagatgagg gtgcatctat
    15241 gattacttat gatttaattg gcaatacacc attagtactg ttagaacatt atagtgatga
    15301 taaagttaaa atttatgcca agcttgaaca atggaatcct ggaggcagtg ttaaagacag
    15361 actcgggaaa tatttagtag agaaggcaat tcaagaaggg cgtgtgcgtg caggtcaaac
    15421 tattgttgaa gcgactgctg gtaatacagg catagggtta gctattgcag cgaatagaca
    15481 tcatttgaaa tgtaagatct ttgcgccgta tggtttttca gaagaaaaga ttaatattat
    15541 gatagcgctt ggtgcagaag tttcaaggac gagtcagtct gaaggtatgc atggggcaca
    15601 attagctgca cgttcctatg ctgaaaaata tggtgccgtt tatatgaatc aatttgaatc
    15661 cgaacataat ccggatacat attttcatac attgggaccc gaattgactt cagcattaca
    15721 gcaaattgat tattttgtgg ctggtattgg ctctggcggt acatttacag gtaccgcacg
    15781 ttatttaaag caacatcacg tgcaatgtta tgccgttgag ccagaagggt ccgtgttaaa
    15841 tggagggcca gctcatgcac atgacactga aggtatcggt tctgagaaat ggccgatatt
    15901 tttagagaga cgtcttgtag atgggatatt tacgattaaa gatcaagatg cctttcgaaa
    15961 tgtcaaaagt ttggctataa atgaagggtt gttagtaggc agttcttcag gtgcagcatt
    16021 acaaggtgca ttgaatttaa aagcgcaatt atctgaaggt acgattgttg tcgtatttcc
    16081 agatggtagt gatcgatata tgtctaagca aatatttaat tatgaggaga ataataatga
    16141 acaagaaaac taaattaatt catggtgggc acacaacaga cgattataca ggtgccgtta
    16201 caacaccaat ttatcaaaca agtacatatt tacaagatga tattggtgat ttacgtcaag
    16261 gatatgaata ttctcgtact gcgaatccaa caagaagttc tgtagaaagc gttattgcga
    16321 cattagaaaa tggcaaacat ggctttgcat ttagttcagg tgttgcagca atcagtgcag
    16381 ttgttatgct gttggacaaa ggagatcata ttattttaaa ttcagatgta tacggcggta
    16441 cttatcgcgc attgacaaaa gtatttacac gatttggcat tgaagtggat tttgtagata
    16501 caacgcatac agattcaatt gtacaagcga tacgcccaac aacaaagatg ttgtttattg
    16561 aaacaccttc taatccatta ttacgtgtta ctgacattaa aaagtctgct gaaattgcga
    16621 aagaacacgg tttgatttca gttgttgata acacatttat gacaccttat tatcagaatc
    16681 cattagattt aggtatcgat attgtcttac attctgcaac gaaatattta ggtggacata
    16741 gtgatgtcgt tgctggttta gttgcaacat cggatgacaa gcttgcagaa cgtttagcat
    16801 ttatttcaaa ttcaacaggt ggcattttag gacctcaaga tagctattta cttgtgaggg
    16861 gtattaaaac attaggttta cgtatggaac aaattaatcg cagcgttatt gaaattatta
    16921 aaatgttaca agcacatcca gctgtgcaac aagtgttcca tccaagtatt gaaagtcatt
    16981 taaatcatga tgtccatatg gctcaagcgg atggccatac aggtgtgatt gcatttgaag
    17041 tgaaaaatac agaaagtgcc aaacaattga ttaaagcaac atcgtattac acattagctg
    17101 aaagtttagg tgcagtggaa agtttaattt cagtacctgc attgatgaca catgcatcca
    17161 ttccagcaga tattcgagct aaagaaggta ttacagacgg acttgtaaga atttctgtag
    17221 gtattgaaga tactgaagat ttagtcgatg atttaaaaca agcactagat actttataaa
    17281 taatagcagc actggcatat attttgagtt caacgtttgt tgttcttgaa tatgctagtg
    17341 cttttttgtg cgatgttaat atttagtaat tgcgttgtgg aggattggtg gacattttta
    17401 tggatttaat ttgataaatg tcatagtagt ctcacaattc gtcattgtca catgaattac
    17461 ttatttttta attttttaga aaattcggca aattgtattg acgatatatg caattttaca
    17521 tataatgctc ttattcctag tggattaata agaatttgta ggaggggcga tgatgattga
    17581 gtttcgacag gtaagtaaga cctttaataa aaagaagcaa aaaatagatg ctttgaagga
    17641 cgtatcattt acggtcaatc gcaatgatat ttttggtgtg attggatata gtggtgcagg
    17701 aaaaagtacg ttggtaagac tcgtgaatca tcttgaagct gcctcgaatg gacaagtgat
    17761 tgtagatgga catgatatta cgaattatag cgataaaatg atgagggata ttaagaaaga
    17821 tatcggtatg atatttcagc atttcaattt attaaattca gctaccgtat ttaaaaatgt
    17881 agcaatgcca ctcattttaa gtaagaaaag caaaacagaa attaagcaac gagtaacgga
    17941 aatgcttgaa tttgtaggat tgagtgataa aaaagaccaa tttcctgatg aattatctgg
    18001 tgggcagaag caaagggtgg ctattgcaag agcgcttgtt actaatccga aaatactcct
    18061 atgcgatgaa gcaacaagcg cattggatcc agcaacgact gcttcgatat tgacgttatt
    18121 aaagaatgtc aatcaaacct ttggcattac aattatgatg attacacatg aaatgcgcgt
    18181 tattaaagac atttgtaatc gtgttgctgt aatggaaaag gggaaagtgg ttgaaacagg
    18241 aactgttaaa gaggtgttta gtcatcctaa aacgacgatt gctcaaaatt ttgtgtctac
    18301 agttatacag actgagccaa gtacatcatt gattcgtcga ttgaatgacg aacaagttgg
    18361 cgattttaaa gattataaaa tcttcgtcga ggaaactcag gtgacacaac cgattataaa
    18421 tgacttgatt caaatttgtg gcagagaggt taaaatttta ttttcatcta tgtcagaaat
    18481 acaaggtaac accgtatgtt atatgtggct tcgatttaat atggatcaac aatttgaaga
    18541 cacggcaata aatcaatatt tcaaagagaa aaatattcaa tttgaggagg tgcattaaca
    18601 tatgtttggt tctgatttag acagtgcaca gttattacaa gcattgtacg aaacgctata
    18661 tatggtatct attgctttat ttttaggagc agtgattggt attccattag gtgtcttatt
    18721 ggtaattact cgaaaacaag gcatatggcc caatatagtg atacatcaag ttttaaatcc
    18781 tttaatcaat attttaaggt cactaccatt tattattttg ttaattgcga ttgtgccatt
    18841 cacaaaatta gtagtaggta cttcaattgg tacgactgct gccatcgtgc ctttaacagt
    18901 atatgtggca ccttacattg caagacttgt tgaaaactca ttattggaag tagacgaggg
    18961 gattattgaa gcggcgaaag cgatgggcgc ttcaccacta caaatcatta gatatttttt
    19021 aattcctgaa gctttaggtt cgttagtatt agcaattacc actgcgatta ttggacttat
    19081 tggaagtacg gcgatggcag gagctgttgg cggtggtggt ataggagact tagctttagt
    19141 gtatggttat caaagatttg atacgacggt cattattatt accgttattg tattagtcat
    19201 tattgtccaa gtgattcaaa cgctagggaa tgttctagct agattcatac gtagacatta
    19261 atgatatata gtgaagattt tgaaaggaat tgatagaatg aaaagattga ttgggttagt
    19321 tatcgtagca cttgtattat tagcagcgtg tggtggtaac aatgataaaa aagtaacaat
    19381 tggtgtcgca tcaaatgaca ctaaggcttg ggagaaggtt aaagaattag ctaaaaaaga
    19441 tgatattgat gtggagatta agcacttctc agattacaat ttaccgaata aagcattaaa
    19501 tgatggtgat attgatatga atgcattcca acattttgca tttttagatc aatataaaaa
    19561 ggcgcataaa ggaacaaaga tttcagcatt aagtacaaca gttttagcac cgttgggcat
    19621 ttactcagat aaaattaaag atgtcaaaaa ggttaaagat ggtgctaaag ttgtcattcc
    19681 aaatgatgtg tcaaaccaag cacgtgcact taaactatta gaagcagctg gtttaataaa
    19741 actgaaaaaa gatttcggat tagcaggcac ggtgaaagat ataacgtcaa atccaaaaca
    19801 tttaaaaatt actgcagtag atgcacaaca aactgcacgt gctttatctg atgtcgatat
    19861 tgcagttatt aataacggtg tagcaactaa agcgggtaaa gatcctaaaa atgatccgat
    19921 atttttagaa aaatcaaatt cagatgcagt aaagccatat attaatattg ttgcagttaa
    19981 tgacaaagac ttggataaca aaacatatgc taaaatcgta gaattgtatc attcaaaaga
    20041 agctcaaaaa gcgttgcagg aagatgtcaa agatggagag aaacctgtta atttatctaa
    20101 agatgagatt aaggcaatag aaacgtcatt agcaaaataa attatattgc gtcctacaag
    20161 caaagttcat gcttatgttt gtagggcgtt attgttggag aataaaatta tttccaatag
    20221 agaaagggat tgtaatcatt ttatagtgaa atattatgaa attgtaataa tttagatatt
    20281 gtaaaatcta ataagttgta ataattttaa ggggtaatta taaaatttga tgatacagta
    20341 tatgattttt ttgtaatcat aatgtcatca aacatcaacc tattatacat aataaaatcg
    20401 tataatgatg tagtattcat aaattcggat aaaagaatgt taggaaagtt aagcaagagg
    20461 aggattttaa agtgcaaaaa aaagtaattg cagctattat tgggacaagc gcgattagcg
    20521 ctgttgcggc aactcaagca aatgcggcta caactcacac agtaaaaccg ggtgaatcag
    20581 tgtgggcaat ttcaaataag tatgggattt cgattgctaa attaaagtca ttaaacaatt
    20641 taacatctaa tctaattttc ccaaaccaag tactaaaagt atctggctca agtaattcta
    20701 cgagtaatag tagccgtcca tcaacgaact caggtggcgg atcatactac acagtacaag
    20761 caggcgactc attatcatta atcgcatcaa aatatggtac aacttaccaa aacattatgc
    20821 gacttaatgg tttaaataat ttctttattt atccaggtca aaaattaaaa gtatcaggta
    20881 ctgctagctc aagtaacgct gcgagcaata gtagccgtcc atcaacgaac tcaggtggcg
    20941 gatcatacta tacagtacaa gcaggtgact cattgtcatt aattgcatca aaatatggta
    21001 caacttatca aaaaattatg agcttaaatg gcttaaataa tttctttata tatccgggtc
    21061 aaaaattgaa agtaactggt aatgcatcta cgaactcagg atctgcaaca acgacaaata
    21121 gaggttacaa tacaccagta ttcagtcacc aaaacttata tacatggggt caatgtacat
    21181 atcatgtatt taatcgtcgt gctgaaattg gtaaaggtat tagtacttat tggtggaatg
    21241 ctaataactg ggataacgca gcggcagcag atggttacac tatcgacaat agacctactg
    21301 taggttctat cgctcaaaca gatgtaggtt actatggtca tgttatgttt gtagaacgtg
    21361 taaataacga tggtagtatt ttagtttcag aaatgaacta ttcagctgca ccaggtattt
    21421 taacttacag aacggtacca gcttaccaag taaataatta tagatatatt cactaaagtc
    21481 ttacgtatat aaatatataa tgaattccta ttacatttca caagctgaaa agttggattg
    21541 aatcctaaat tttatggcgg ggctatcaaa gtcgtgattg ttgtaatagg aatttttgta
    21601 tatgaaaaag gattggtcga atgaacttca tgttctatgt tcgaccaatc cttttctgaa
    21661 ttagtattca tgttttactt tgcgaatgct tgtaaataat ctagcaccgt ttgttattaa
    21721 agtaacaact gccactgctt ttttgaactt acgtggtgac ttaattgaaa tgtaaaagtc
    21781 taacacggtt cctacagtgc ttaaacctaa tgaaagatat actaattttt tatttttagc
    21841 atgatattta tagccattgt agccgtcgac tatgaaacct gcgacattta gtaaacttga
    21901 taaacgttgt gatttggaac gttttgccat aataataatt ccccctagta tcttctgttt
    21961 atatgaaaag ttgggtgcgc tgaattgcta acgttttgcg ctataactac tcatatatga
    22021 taacataatt gtacagtata atttgaaaaa ttgatttcac aaagttgggg tgtcaaagat
    22081 gattaaatgt gtctgtttag ttgaagaaac agctgataaa atattgcttg ttcaagtaag
    22141 gaatcgcgaa aagtattatt tcccgggtgg taaaatagaa gaaggggaat cacaagtaca
    22201 cgcgctgtta agagaagtaa aagaagaatt aaatttaaca ttaacaatgg atgaaattga
    22261 atatgtcggg acaattgtag gtcctgcata tccacaacag gatatgttaa ctgagttaaa
    22321 tggatttcgc gcattaacca aaatcgattg ggaaaacgta actatcaata atgaaattac
    22381 ggatatacgc tggattgata aagataatga tgcgttgatt gcgcctgctg tcaaagtttg
    22441 gattgaaact tatggtggta aacatgacaa ataatgacac catcatgtta cgacattatg
    22501 tcccacaaga ttattcgatg ttagaagctt ttcaattaag tgaaagtgat ttgaagtttg
    22561 ttaaaacgcc agaggaaaat attacagctg caatgtctga taatgaaagg tatcccatcg
    22621 ttgtaatgga tggcaggcaa tgtgtggcct tttttacatt acatcgtgga aaaggggtcg
    22681 caccatttag cgataaccaa gatgcagtat ttttcaggtc atttagtgtt gatcaacgtt
    22741 atcgtaatag aggaataggt aaagtggtaa tggaaaaatt ggcgtcattt atcacttcaa
    22801 catttcagga tattaatgag attgtgttaa cggttaatac tgacaatcca catgccatgg
    22861 cactttatcg ccaacaagga tatcaatata tgggagatag tatgttcgtc ggaagacctg
    22921 ttcatattat ggcgttaact ataaaataaa ttaaatttaa aagcatcttt actcatcgtc
    22981 gaccacaaca attaatgatg aataaaggtg ctttttgtta tagatcatcg gacaatttac
    23041 tatagtaaaa agcgacctag tgaacaattg acatatatcc acaggtcgct taacttaagt
    23101 tatattgcta gttgcgatta attgatagac tcatcatttt tgcgctgtcg agatggtctt
    23161 tttattaaaa atgccgtaat ccaagccgta atcggaatac tgattgcaac ggcaataccg
    23221 cctaaaataa tagaaataaa ttcttgggca aatattttcg agtttataat atgaccaaat
    23281 gaatatttaa gtttgaaaaa ccaaataaat aaagcaagtt ggccaccaaa aaaggcaagg
    23341 taaatcgtgt tcgcagatgt cgctaaaatt tctctaccaa cacgcatgcc agattggaat
    23401 aattcgtatt gcgtaagcgt tggattcact tgatgcaatt cataaatggg tgaactaatg
    23461 gtaattgtta aatctatcac agctgcaata acagcaagaa taatagtgaa caccataaat
    23521 tgaaccatat caatgccaat attcattgaa tacacatatg tttcatcttg ttgttcggtt
    23581 gaaaagcctt gtagatgacc gaagtagacc gataaataaa tgagtgtaat caacaatatt
    23641 gttgtaacga tagtgctgat aaatgcagct tgtgttttaa cattgtaact attgagtacg
    23701 aataaattac aagcgccaat aataatgcag aaaaagaatg tgacgacata aatcggtacg
    23761 ccaaaaataa tcaatacaat actaataatt aaaatagcga aatttaaaaa tagggttaaa
    23821 taagagatga atcccttttt acctccgaaa attatcatca gaaagaggag caataacgcc
    23881 aatataaata cagcattcat tgtttcgccc tccttaatgt ttcaaatatt tccataaaca
    23941 atattgtgat aggaattgta agtacgatac ctataccacc tgttagtgcg cgcgcaattt
    24001 ctaacgacca attcatcgaa atagtataag tcactgtatt ggcatttttt aaaaagatta
    24061 aaaacatagg tagtgcaccg gataaatatg agaataataa gatgttagtc attgttccca
    24121 taatatcttg gccgatgttt cgcccagcaa gcgcccatct cctcattgaa atgtgtggcg
    24181 tacgctgtaa aatttcatgc ataccactag caattgtaat tgcaacatcc ataatagcgc
    24241 caagtgaacc tattaacact gaggctagga agatatcttt cggtggtaat gataaaaagt
    24301 tcatcgtttc atatttaatg cctttaccat ctgtcatata tatgattaat tctgttaaac
    24361 ctatactcaa aaaagttccg ataattgtac tggctatggt gatgagtgta cgcatatgcc
    24421 agcctgtaac gagcaataaa gtgagtattg ttgaacagat catggcaatg gtcatgagta
    24481 agaataaatt aatattgcta tgttgaatat gaatgtaaat tgcgattaat atggcaatag
    24541 aattcaagat taacgataaa atcgattgca gtccgacttt gcgaccaacc aataatacag
    24601 ttaataagaa caaaccagtg atgataaccg ttaaggtatc acgcttcttt tctataatat
    24661 aagcatcact cggcttgtta gaaatatgta ataatacttt ttcgtgtgtg cgaaatgcct
    24721 cagaatctgc ttgcgatttg acgtactgat gattaatcgt cgtcgtttct ccagcaaatt
    24781 gaccatttaa tattttgact tttaattgat ttttatattt aatatcacga ttattttgtg
    24841 catcttttgt aggtgtcgaa gaaacatgtt tgacatctat aatttgacca attggtttgt
    24901 tgtaaaagtt ctcattattg aatgtaaata aaatagcacc aatgaatgcg atgcagaaca
    24961 aacctaaaat tatattaaat ggctttgtaa ataaatttct atatttcaaa aacaaaaccc
    25021 caattctatg aatgaattaa tatggtgatt atacgccctt aattttttat tttcaaagat
    25081 attactgcta agtgtaaaac gaaaatcatc attgatagca tcgaattact taatggaatg
    25141 tagacgtttt agtcattaat tgctgaataa gtgttaataa tatgccaata tcactctttg
    25201 tataaggctc ctttgtaata gcacatatcg ttctttttaa ttcagtatga tctaatttta
    25261 tatctatcca tgatttagat tctggtaaat gtatattttg tgatgaaatg atgtaacctt
    25321 ctttttgacg aaggagatac tgcgcaagtg gttggctact gattgtgtat acatctgatt
    25381 tagtaatctt gcgcaattgt ttttttacag tttcggcaaa tggtgccaag caataaatat
    25441 gactatgctc aaactgaatt aatggtgggt gtgtcgccat cgtaattgga tcgtctgaag
    25501 gcgcatataa atgatagtgc tcttcgaata aaggtagcat atgtaattgt ttgtgtttac
    25561 gtatttctgg tgtaagttcc gtgaaaccaa tgtctatatt cccatttaat acgctattta
    25621 taattgtgtc atgttctaat aagctcggta tgacatgtgt atcattttgt aaatgaaacg
    25681 tttggataag tggtagtaac atgtgggata cgtcactctc atcatagcca atgtagatac
    25741 ttttattttt agttaatcca tggctttgaa attgttcaat cgtgctatct aaatgttcaa
    25801 taatacgcag agcttcatta aataataatt tcccttcaga tgtgagcgta atattgcgtc
    25861 cttgcttttt aaataaagac acattaagtt cttgttctaa taatgtaatt tgacggctta
    25921 tcgctgattg agcaatgttt agttcaagtg ctgtttcgga gatatgttct cttttagcga
    25981 cctcgataaa atatcttaat tgtttaattt ccatagcgat ataggcacct ccaaaaatga
    26041 gtgttttgta actattatag caatattatt gataaatgtt ctatttttta gatgaatatc
    26101 ttctatttta tatattgaac agataaattt tttagattat agtaattatc attaataact
    26161 aatatcagaa tattctaaaa aaggggtgtg catcatgcac aatgagaaat taattaaagg
    26221 cttatatgac tatcgtgagg aacatgatgc gtgtggtatt ggtttttatg cgaatatgga
    26281 taataaaagg tctcacgaca tcattgataa atcgcttgaa atgttgcgac gcttagatca
    26341 caggggcggg gtcggcgcag atggcatcac tggtgatggc gcaggtatta tgactgaaat
    26401 accttttgca tttttcaaac aacatgtaac ggactttgat atcccaggtg aaggtgaata
    26461 tgccgtgggg ttattttttt ccaaagaacg cattttaggt tctgaacatg aagtagtttt
    26521 taaaaaatat tttgaaggcg aagggttatc aattcttggt tatcgtaatg taccagttaa
    26581 taaagatgcc attgctaaac atgtagcaga tacgatgcca gtcattcaac aagtgtttat
    26641 tgatattagg gacattgaag atgttgaaaa gcgtttgttt ttagcgagaa aacaattaga
    26701 gttctattcg actcagtgcg atttagaatt gtattttacg agcttatcac gcaaaacaat
    26761 tgtatataaa ggttggttac gatcagacca aattaaaaaa ctatatacag atttatcgga
    26821 tgatttatat caatcaaagc tagggttagt gcattcgaga tttagtacga atacattccc
    26881 gagttggaaa agggcacatc ctaaccgtat gttaatgcat aatggtgaga ttaacacgat
    26941 taaaggtaat gtaaactgga tgcgagcacg ccaacataaa ttaatcgaaa cattatttgg
    27001 cgaggatcaa cataaagtgt ttcaaattgt cgatgaggat ggtagtgact ctgccattgt
    27061 agataatgcg ctagagttct tatcgttagc catggagcca gaaaaggcag cgatgttact
    27121 catacctgaa ccttggttat ataatgaagc gaatgatgca aatgtacgtg cgttttatga
    27181 attttatagt tatttaatgg aaccgtggga tggtcctaca atgatttcgt tctgtaacgg
    27241 tgacaaactt ggcgcgctta cagatagaaa tggattacgt ccaggtcgtt atacgattac
    27301 taaagataac tttattgtct tttcatctga agtgggtgtt gtggacgtac ctgaaagtaa
    27361 tgttgctttt aaaggtcaat tgaatcctgg aaagttattg cttgttgatt ttaaacagaa
    27421 taaagtcatt gaaaataatg atttaaaagg tgcgattgct ggagaattac catataaagc
    27481 gtggattgat aaccataaag ttgactttga ttttgaaaat atacaatatc aagattcgca
    27541 atggaaagat gagacgttat ttaaattaca acgtcagttt gcatacacga aagaagagat
    27601 tcataagtat attcaggaac ttgtagaagg taagaaggat cctatcggtg caatgggata
    27661 tgatgcgcca attgcagtgt tgaacgagcg accagaatca ctatttaatt actttaaaca
    27721 gctgtttgca caagttacga atccaccaat tgatgcgtat cgtgaaaaaa tcgtaacgag
    27781 tgaactttct tatttaggtg gcgaaggtaa cttactagca cctgacgaaa cggttttaga
    27841 tcgtattcaa ttgaaaaggc cggtattgaa tgaatcacac ttagcagcga ttgatcagga
    27901 acattttaaa ttaacttatt tatcaacggt atatgaaggg gatttggaag atgcgttaga
    27961 agcattaggc cgagaagcag tgaatgctgt aaagcaaggc gctcaaattc tagtgttaga
    28021 tgatagtgga ttagttgata gcaatggctt tgcaatgccg atgttactcg caataagtca
    28081 tgtgcatcaa ttacttatta aagcagattt acgtatgtct acaagtttag tcgctaaatc
    28141 tggtgagaca cgagaagtgc atcatgttgc ttgtttactc gcatatggcg cgaatgcaat
    28201 tgtgccatac ctagcgcaac gtacagttga acaactgaca ttgacagaag ggttacaagg
    28261 caccgttgtc gataatgtta agacatatac ggatgtattg tcagaaggtg tcattaaagt
    28321 aatggctaag atgggaattt cgacagtgca aagttatcaa ggggcacaaa tatttgaagc
    28381 gattggcttg tctcatgatg tgattgatcg ttattttact gggacacagt ctaagttatc
    28441 tggtatttcg attgatcaaa ttgatgctga aaataaagca cgtcaacaaa gtgatgataa
    28501 ttatcttgca tcaggtagta cattccaatg gagacaacaa ggtcaacatc atgcttttaa
    28561 tccggaatct attttcttat tgcagcacgc atgtaaagaa aatgactatg cgcaatttaa
    28621 agcatactct gaagcggtga acaaaaatag aacagatcac attagacatt tacttgaatt
    28681 taaagcatgt acaccgattg acatcgacca agttgaaccg gtaagtgaca ttgtcaaacg
    28741 ctttaataca ggggcgatga gttatggatc gatttcagcg gaagcacatg aaacgttagc
    28801 acaagccatg aaccaattag gtggaaagag taatagtggt gaaggtggcg aagatgcaaa
    28861 acgttatgaa gtacaagttg atggaagcaa caaagtaagt gcgattaaac aagttgcttc
    28921 tgggcgtttt ggtgtaacta gtgattattt acaacatgcc aaagaaattc aaattaaagt
    28981 tgcgcaaggt gcaaagcctg gtgaaggtgg tcaattacct ggtactaagg tatatccgtg
    29041 gattgcgaag acaagagggt caacgccagg tatcggtctg atttcaccac cgccacatca
    29101 tgatatttat tcaatagaag atttagcgca actgatacat gatttgaaaa atgcgaataa
    29161 agatgcagat atcgcggtaa aattagtttc gaaaacaggt gttggtacca ttgcatctgg
    29221 ggtggcaaaa gcatttgcag ataaaattgt catcagtggt tacgatggtg gtacaggggc
    29281 ttcacccaaa acgagtattc agcatgccgg tgttccttgg gagattggtt tagcagaaac
    29341 acatcaaaca ttaaaactaa atgacttaag aagtcgtgtt aagttagaaa cagacggtaa
    29401 gttattaact ggtaaagatg tagcgtacgc atgtgcgctt ggagcggaag aatttggatt
    29461 tgcaactgca ccattagtgg tgttgggctg tattatgatg cgtgtatgcc ataaagatac
    29521 atgtccagta ggagttgcaa ctcaaaacaa agatttacgt gctttatata gaggtaaagc
    29581 acatcatgtt gttaatttta tgcattttat tgcacaagaa ttaagagaaa ttttagcatc
    29641 tttaggtttg aaacgtgtag aagacttagt tggaagaact gatttattac aacgatcatc
    29701 aacattaaaa gcgaatagca aagcggctag tattgatgtt gaaaaactgt tatgtccttt
    29761 cgatgggcca aacacaaaag aaattcaaca aaatcataat cttgagcatg gatttgattt
    29821 aacaaattta tatgaagtaa cgaagccata tattgctgaa gggcgtcgct atacaggtag
    29881 ctttacagta aataatgaac aacgtgatgt aggggttatt acaggtagtg agatttcgaa
    29941 acaatatgga gaagcaggac ttcctgaaaa tacaattaat gtttatacga atggtcatgc
    30001 tggtcaaagt cttgcagcat atgcaccgaa aggcttaatg attcatcata ctggagatgc
    30061 gaatgactat gttggtaaag gattatctgg tggtacggtc attgtcaaag caccttttga
    30121 agaacgacaa aatgaaatta ttgctggtaa cgtctcattc tatggtgcga caagtggtaa
    30181 ggcatttatt aacggtagtg caggagaaag attctgtatt agaaatagtg gtgtagatgt
    30241 tgtcgttgaa ggtatcggcg accatggatt agagtatatg actggtggac atgtcattaa
    30301 tttaggtgat gtaggtaaga acttcggtca aggtatgagt ggtggtattg cttacgttat
    30361 cccgtctgat gtagaagctt ttgttgaaaa taatcaacta gatacgcttt cgtttacaaa
    30421 gattaaacac caagaagaaa aagcattcat taagcaaatg ctggaagaac atgtgtcaca
    30481 cacgaatagt acgagagcga ttcatgtgtt aaaacatttt gatcgcattg aagatgtcgt
    30541 cgttaaagtt attcctaaag attatcaatt aatgatgcaa aaaattcatt tgcacaaatc
    30601 attacatgac aatgaagatg aagcgatgtt agctgcattt tacgatgaca gtaaaacaat
    30661 cgatgctaaa cataaaccag ccgttgtgta ttaaggaaag ggggagatac gatgggtgaa
    30721 tttaaaggat ttatgaagta tgacaaacag tacttaggtg aattatcact ggtagaccgt
    30781 ttgaagcatc ataaagcata tcaacaacga tttactaaag aagatgcctc tatccaaggt
    30841 gcacgatgta tggattgtgg aacgccgttt tgtcaaaccg gacaacagta tggtagggaa
    30901 acaataggtt gtccaattgg aaactacatt cctgaatgga acgacttagt gtatcatcaa
    30961 gattttaaaa ctgcttatga acgcttaagc gaaacaaata actttcctga ctttacaggg
    31021 cgtgtatgtc ctgcaccatg cgaaagtgct tgtgtgatga agattaatag agaatcgatt
    31081 gcgattaaag gtattgaacg cacaattatt gatgaagctt ttgaaaatgg ttgggtagcg
    31141 ccgaaagttc cgagtcgccg tagagatgaa aaagtggcaa tcgttggaag cggtccagca
    31201 ggattagctg ctgctgaaga acttaatcta ctaggatatc aagtaactat ttatgaacgt
    31261 gctagagaat caggcggttt attaatgtat ggtattccga atatgaaact tgataaagat
    31321 gtggttcgac gtcgtattaa gttaatggaa gaagcgggca ttactttcat taatggtgtt
    31381 gaagtcggcg ttgatattga taaagcaacg ttagaatctg agtatgatgc cattatatta
    31441 tgtactggtg cacaaaaagg tagagattta cctttagaag gacgcatggg tgatggtata
    31501 catttcgcta tggattattt aactgaacaa acgcagttgt taaatggaga aattgatgat
    31561 ataacaataa ctgcaaaaga taagaatgtc attatcattg gtgctggtga tacaggggca
    31621 gactgtgtag cgacagcatt aagagaaaat tgtaaatcga ttgttcaatt taataaatat
    31681 acgaaattgc cagaagcaat tacatttaca gaaaatgcat catggccttt agcaatgccg
    31741 gtgtttaaaa tggactatgc gcaccaagag tacgaagcta agtttggtaa ggaaccacgt
    31801 gcatatggtg ttcaaacaat gcgttacgat gttgacgata aaggacacat acgtggtttg
    31861 tatactcaaa ttttagagca aggcgaaaat ggtatggtca tgaaagaagg acctgaaaga
    31921 ttttggcctg ctgaccttgt attattatca atcggcttcg aaggtacaga accaacagta
    31981 ccgaatgctt ttaacattaa aacggataga aatcgaatcg tggcggatga tacaaactat
    32041 caaactaata atgaaaaggt atttgctgct ggagatgcta gacgtggtca aagtttagtt
    32101 gtatgggcaa ttaaagaagg tagaggcgta gcgaaagcag tagatcagta tttagctagt
    32161 aaagtttgtg tataatcttt gtatggaaat ggtggttacg ttgacgttgt gacatgctga
    32221 atcgagtttg aaaaaatcta gtatctatca acgtcacatg ccatctttgt aacctaaaaa
    32281 caaaggtttg taagacaaca aatagattaa ttataagtag tgatttttta cattcgttta
    32341 taggtcaact gtagtggaag acaatgattt gtggtaatca tgtaatgctt aaaaacaata
    32401 ttgactttta cagaacgttc atatatgata aatattgtgt ttaggaggaa tacccaagtc
    32461 cggctgaagg gatcggtctt gaaaaccgac aggggcttaa cggctcgcgg gggttcgaat
    32521 ccctcttcct ccgccatcaa tatttatatt aaattctata tataatgaag gtaagtgctc
    32581 aaattttgag tatttacctt ttttatttgt ctttgaatgg ctcgtaattt ttgataatag
    32641 aaatgataag gcattgagat tggaagggca tttggcttgt gcaatataca tagctaaatg
    32701 tcttttttgt tttgtgaaat atgatggatg gcttgtgtgg acaagtttgc tatttataga
    32761 tatgcatttt tcaatttagg agttggccat gcatctacac tttataatgg tgagagcgtg
    32821 gtgaggtatt gttaataacg caattgtagc gaggagttat tgctacatat gtcgttatgg
    32881 ctcattgatt ttctgaaatg gctaccccag ataattgtga caaaataaaa atattttgtt
    32941 gaaagccttt acataacttg tctagacaag ttatactcgt tttaagacat taagggagtg
    33001 aaatatatgg ctgtaaaaag agaagatgta aaagccatcg taaccgctat tgggggaaaa
    33061 gaaaatcttg aagctgcaac gcattgtgta acacgattat gtttagtgct gaaggatgaa
    33121 agtaaagttg ataaagacgc attaagtaat aacgcgttgg tcaaggggca gtttaaagca
    33181 gaccatcaat atcaaattgt cattggtcca ggaacagtcg atgaagtgta taagcagttt
    33241 attgatgaaa caggtgctca agaagcttcg aaagatgaag cgaaacaagc agctgcacaa
    33301 aaagggaatc cagtacaacg tttgatcaaa ttgttggggg atatttttat accaatatta
    33361 cctgcgattg tgacagctgg tttgttaatg ggaatcaata atttacttac aatgaaaggt
    33421 ttatttggtc caaaagcact tattgagatg tatccacaaa ttgctgatat ttcaaacatc
    33481 attaatgtga ttgcgagtac ggcatttatt ttcttaccag cattaattgg ttggagtagt
    33541 atgcgtgtat ttggtggtag tccgattcta ggcatagtct taggtttgat tttaatgcat
    33601 ccgcaattag tatctcagta tgatttggca aaagggaata ttccgacgtg gaacttattt
    33661 ggcttagaga ttaagcagtt gaattaccaa ggtcaagtgt tgccagtttt aattgcagct
    33721 tacgttctag ctaaaattga aaaaggatta aataaagtcg ttcacgattc gataaaaatg
    33781 ttggtcgttg gacccgtagc gcttttagtt actggatttt tagcatttat tatcattgga
    33841 ccagttgcgt tattgattgg tacaggtatt acatctggtg ttacatttat attccaacat
    33901 gcaggatggc ttggcggagc aatatatgga ttgttatatg caccacttgt aattacagga
    33961 ctacaccata tgtttttagc agtagatttc caattgatgg gtagcagctt aggcggtacg
    34021 tatttatggc caattgttgc gatttccaat atttgtcagg gctctgcagc atttggagca
    34081 tggtttgtct ataaacgtcg taaaatggtt aaagaagaag gcttggcatt aacatcttgt
    34141 atttctggta tgttaggtgt tactgaacca gccatgttcg gtgtgaactt acctctgaaa
    34201 tatccattta tcgctgcgat atcaacgtct tgtgtattgg gggcaatcgt tggtatgaat
    34261 aacgtacttg gaaaagttgg tgttggtggc gtgccagcat tcatttcaat tcaaaaagaa
    34321 ttttggccag tatatcttat tgtgacagct attgctattg ttgtaccatg tatactaaca
    34381 attgtgatgt ctcattttag taaacaaaaa gcgaaagaaa ttgttgaaga ttaataaaat
    34441 aaaaaagggg cgttcgttat ttggacgtcc tttattacgt tataaggtgg taattgtgtg
    34501 tcgaaagaaa tagattggag aaaatccgtt gtatatcaaa tttatcctaa gtcgtttaat
    34561 gatacgacgg ggaatggtat aggagatatc aatggaatta tagaaaaatt ggattatatc
    34621 aagttattgg gtgttgatta tatttggtta acaccagtgt atgaatcacc gatgaatgat
    34681 aatggctatg atatcagcaa ttatttagaa atcaatgaag actttggaac gatggatgat
    34741 tttgaaaagt taatcaaagt tgcgcatcaa aaagacttga aagtgatgtt agatattgtc
    34801 attaatcata cgtcgacgga gcatgaatgg tttaaagaag cccgtaaatc taaagataac
    34861 ccttatagag attattactt tttcagatca tctgaagacg ggccgccaac aaattggcat
    34921 tctaaattcg gtggtaatgc atggaagtat gattctgaga cagatgaata ttatttacat
    34981 ttatttgatg tcagtcaagc tgatttaaat tgggataatc cggaagtacg tcaatcgtta
    35041 tatcgcatag tcaatcattg gatagacttc ggcgttgatg gttttcgatt tgatgtcatt
    35101 aacttaattt ctaaaggtga atttaaggac tctgacaaaa taggtaaaga attttatacg
    35161 gatggtccta gagtgcatga gtttctgcat gaattaaatc gtcaaacgtt tggtaacact
    35221 gacatgatga ctataggaga aatgtcttcg acgacgattg aaaattgtat taagtataca
    35281 caaccagaac gccaagaatt gaatagtgtt tttaattttc atcatctaaa ggttgattat
    35341 gttgatggtg aaaagtggac aaatgcgaag cttgattttc ataagttaaa ggaaattctg
    35401 atgcaatggc aacgaggtat ttatgacggt ggcggatgga acgcgatttt ctggtgtaat
    35461 catgatcagc cacgggtagt gtctagattt ggtgatgata cgtcggaaga gatgaggata
    35521 caaagtgcta aaatgttagc tatcgcactg catatgttgc aagggacgcc atatatttac
    35581 caaggtgaag aaattggtat gacggaccca cattttacat caatagcaca atatcgtgat
    35641 gttgaatcga ttaatgccta ccatcagttg ttaagtgaag ggcatgctga agcggatgtg
    35701 ttagcgattt taggacagaa gtcacgagac aattcgagaa cgcctatgca atggagtgat
    35761 gatgttaatg ctggatttac agctggtaag ccttggattg atatttcgga aaattatcat
    35821 caggtcaacg ttagacaagc acttcagaat aaagagtcta ttttctatac gtatcaaaaa
    35881 ttaatacaat taagacatac gcatgatatt attacgtatg gagacattgt gccacgtttt
    35941 atggatcatg atcatttatt tgtttatgaa cgtcattata agaatcaaca atggctagta
    36001 attgcgaatt tctcagcatc ggctgttgat ttgccagaag gattggctag agaaggttgt
    36061 gttgtgattc aaacaggcac agtggaaaat aatacgataa gcgggtttgg tgcaattgta
    36121 atcgaaacaa acgcgtaaaa taaattgagt ggatgcgttt atatggcgaa acaaaaaaag
    36181 tttatgaaga tttatgaggc gttgaaagaa gatatattaa acgggcagat tcaatatggt
    36241 gaacaaattc cgtctgaaca tgatttggtg caattgtacc agtcatctcg agagaccgtg
    36301 cgtaaggcat tagatttgtt ggcattagac ggcatgattc aaaagattca tggtaaaggg
    36361 tcacttgtca tttatcagga ggttacagag tttccatttt ctgaacttgt tagttttaaa
    36421 gaaatgcaag aagaaatggg cgtcgcatat ttaactgaag ttgttgtgaa tgaggttgtt
    36481 gaagcgcatg aagttccaga agttcaacat gctttaaaca tcaattctag tgaatcactc
    36541 attcatattg ttagaactcg tcggcttaac caacatgtga agattgttga tgaagattat
    36601 tttctaaagt cgattgtttc agatataggt aatgatgttg cgagtgattc tatttatgat
    36661 tatttggaaa aggtattaaa tcttaatatt agttattcaa gtaagtctat tacttttgaa
    36721 ccgtttgatg aacaagcata tcaattgttt ggtgatgtat cggtggctta ttcagcaaca
    36781 gttcgaagta ttgtgtattt agaaaataca atgccttttc aatataatat ttcaaaacat
    36841 cttgcaaatg aatttaaatt taatgacttc tcaagacgtc gtataaagta aacaatgata
    36901 taaatgattt atacttgcaa ttaactatta aaatatagta atatatatct tgccgtgcta
    36961 ggtggggagg tagcggttcc ctgtactcga aatccgcttt atgcgaggct taattccttt
    37021 gttgaggccg tatttttgcg aagtctgccc aaagcacgta gtgtttgaag atttcggtcc
    37081 tatgcaatat gaacccatga accatgtcag gtcctgacgg aagcagcatt aagtggatca
    37141 tcatatgtgc cgtagggtag ccgagattta gctaacgact ttggttacgt tcgtgaatta
    37201 cgttcgatgc ttaggtgcac ggttttttat tttttaaata ttaaaccgat tattaagagt
    37261 tgaaaatata taattataga agctactttc ttgaagacaa ttcagcgtat tatacgtgga
    37321 acatgtttgt gggaagtagc ttttttatat gtgaagtttg attcaagtga actcgatgtg
    37381 cagtttgaat gatttttgtg tcaatgaaaa gtaagaagtt ataatttgat gataaagaaa
    37441 tgatggtgaa atgaggggga gtatcttaca atagaattat taatgagata cgttatgatt
    37501 attgacaatc aaatgcctac ggaggacata tgcaaatata tttaagtact ttaacagagt
    37561 tagattatga taaatcttta aatagtattg aagaaagttt tgatgataat cctgaaacga
    37621 gttggcaagc acgtgcgaaa gtaaaacatt taagaaaatc tccttgctat aattttgaat
    37681 tagaagtaat agcgaaaaat gaaaataacg atgtcgttgg acacgtttta ttaattgaag
    37741 tagaaattaa tagtgatgat aagacgtatt atggtttggc gattgcctct ttatcagttc
    37801 atcctgaatt acgtggacaa aaattaggtc gtggcttggt tcaagcagta gaagagcgtg
    37861 ccaaagcaca agagtatagt acggttgttg tagaccattg ttttgactac tttgaaaagt
    37921 tgggttatca aaatgctgct gagcatgaca ttaaattaga atctggtgat gcaccgttac
    37981 ttgtaaaata tttatgggat aatttgacgg atgcaccaca cggaatcgta aaatttccag
    38041 aacattttta ttaattgttc aattaagaag taaaggtatt atcatgctat aatgagaggt
    38101 aattgtttat ggaggtgcta acttgaatta tcaagcctta tatcgtatgt acagacccca
    38161 aagtttcgag gatgtcgtcg gacaagaaca tgtcacgaag acattgcgca atgcgatttc
    38221 gaaagaaaaa cagtcgcatg cttatatttt tagtggtccg agaggtacgg ggaaaacgag
    38281 tattgccaaa gtgtttgcta aagcaatcaa ctgtctaaat agcactgatg gagaaccttg
    38341 taatgaatgt catatttgta aaggcattac gcaggggact aattcagatg tgatagaaat
    38401 tgatgctgct agtaataatg gcgttgatga aataagaaat attagagaca aagttaaata
    38461 tgcaccaagt gaatcgaaat ataaagttta tattatagat gaggtgcaca tgctaacaac
    38521 aggtgctttt aatgcccttt taaagacgtt agaagaacct ccagcacacg ctatttttat
    38581 attggcaacg acagaaccac ataaaatccc tccaacaatc atttctaggg cacaacgttt
    38641 tgattttaaa gcaattagcc tagatcaaat tgttgaacgt ttaaaatttg tagcagatgc
    38701 acaacaaatt gaatgtgaag atgaagcctt ggcatttatc gctaaagcgt ctgaaggggg
    38761 tatgcgtgat gcattaagta ttatggatca ggctattgca tttggtgatg gtacgttaac
    38821 attgcaagat gcgttgaatg tcacaggtag cgtacatgat gaagcgttgg atcacttgtt
    38881 tgatgatatt gtacaaggtg acgtacaagc atcttttaaa aaataccatc agtttataac
    38941 agaaggtaaa gaagtgaatc gcctaataaa tgatatgatt tattttgtca gagatacgat
    39001 tatgaataaa acatctgaga aagatactga gtatcgagca ctgatgaact tagaattaga
    39061 tatgttatat caaatgattg atcttattaa tgatacatta gtgtcgattc gttttagtgt
    39121 gaatcaaaac gttcattttg aagtgttgtt agtaaaatta gctgagcaga ttaagggtca
    39181 accacaagtg attgcgaatg tagctgaacc agcacaaatt gcttcatcgc caaacacaga
    39241 tgtattgttg caacgtatgg aacagttaga gcaagaacta aaaacactaa aagcacaagg
    39301 agtgagtgtc gctcctgttc aaaaatcttc gaaaaagcct gcgagaggca tacaaaaatc
    39361 taaaaatgca ttttcaatgc aacaaattgc aaaagtgcta gataaagcga ataaggcaga
    39421 tatcaaattg ttgaaagatc attggcaaga agtgattgat catgccaaaa ataatgataa
    39481 aaaatcactc gttagtttat tgcaaaattc ggaacctgtg gcggcaagtg aagatcacgt
    39541 acttgtgaaa tttgaggaag agatccattg tgaaatcgtc aataaagacg acgagaaacg
    39601 tagtagtata gaaagtgttg tatgtaatat cgttaataaa aacgttaaag ttgttggtgt
    39661 accatcagat caatggcaaa gagttcgaac ggaatattta caaaatcgta aaaacgaagg
    39721 cgatgatatg ccaaagcaac aagcacaaca aacagatatt gctcaaaaag caaaagatct
    39781 tttcggtgaa gaaactgtac atgtgataga tgaagagtga tacatgacaa gcgatataat
    39841 cgtatgtata atgaaagaaa catcatttta ttgataaata tttattgatt ttcaaggagg
    39901 aaatggaata tgcgcggtgg cggaaacatg caacaaatga tgaaacaaat gcaaaaaatg
    39961 caaaagaaaa tggctcaaga acaagaaaaa cttaaagaag agcgtattgt aggaacagct
    40021 ggcggtggca tggttgcagt tactgtaact ggtcataaag aagttgtcga cgttgaaatc
    40081 aaagaagaag ctgtagaccc agacgatatt gaaatgctac aagacttagt gttagcagct
    40141 actaatgaag cgatgaataa agctgatgag cttactcaag aacgtttagg taaacatact
    40201 caaggcttaa acatccctgg aatgtgatca tagatgcatt atccagaacc tatatcaaaa
    40261 cttattgata gctttatgaa attgccaggc attggtccaa agacagccca acgtctggct
    40321 tttcatacct tagatatgaa agaagacgat gttgttcagt ttgccaaagc attagtagat
    40381 gttaagagag aattaacata ttgtagcgta tgtggtcaca ttactgaaaa tgatccatgt
    40441 tatatttgtg aagataagca aagagatcgt tcagttattt gtgttgtgga agatgacaaa
    40501 gatgtcatag ctatggaaaa aatgagagaa tacaaaggtt tatatcacgt tttacatggg
    40561 tctatttcgc ctatggatgg cattggacca gaagatatta atattccttc attgattgaa
    40621 cgcttgaaaa acgatgaagt tagcgaatta atcttagcta tgaacccgaa cttagagggg
    40681 gaatctacag ccatgtatat ttctagatta gttaagccta taggtatcaa agtgacgaga
    40741 ttagcacaag ggttatcggt aggtggcgat ttagagtatg ctgacgaagt aacattatct
    40801 aaagcaatcg caggtagaac agaaatgtaa tgtcttctat taaacatttt tgattttaat
    40861 actatagtaa gaaaagtcac agtgtaatca ttgtggcttt ttttatggtg tggtgtgatg
    40921 tactacttta tttgcggtgt ggcggtggta tggtttacct agttttactg agggatgggt
    40981 aatctttagg aagcaagccg ttggttgtga tttgttactt ctaatagtaa tgatgtgaat
    41041 tggattatcg aattagatct atggttatgg tgtgttggtg ctattaattt gataaatgcg
    41101 gttaatgact atgcaaatga aattcttttg taattgaaat gatagatgct ggcttagtaa
    41161 gttgtacttc tttggtctaa agcttattaa atcagcctgt atagcggtgt tttgagagat
    41221 tatttaaaac ttgtaaattt atttttaatt tctggtaaaa aaataacgtt ctgttttgcg
    41281 tttttttgat tgatatggtt agagaaaaat ctgtttcttg ttctaaaaaa cgtactattt
    41341 ataagtgggg gttttttaag ttcgattttt aggataaggg cgttcagtac agatgacaaa
    41401 ggtgtaattt ttactgttgt taagcagttt gaaagcctgt atagtattta tttgttgagg
    41461 caaacaaaac aactcaactt aagaaataac ttgaattact aacgaaaatt aattttaaaa
    41521 agttattgac ttaaatgtta ataaaatgta taattaattc ttgtcggtaa gaaaaatga
//
LOCUS       DAXWFK010000006        40513 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.006, whole genome shotgun sequence.
ACCESSION   DAXWFK010000006 DAXWFK010000000
VERSION     DAXWFK010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 40513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40513
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.006"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..228)
                     /locus_tag="ACGEXS_000194"
     CDS             complement(<1..228)
                     /locus_tag="ACGEXS_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011243.1"
                     /translation="MEENKTLKEYLRNFLEGYKYVVENRYIYQFSSNPEAFPFMRKDD
                     YKISIFYLNQSFFEEPCIVVISNDSKLKEIYN"
     gene            complement(260..985)
                     /locus_tag="ACGEXS_000195"
     CDS             complement(260..985)
                     /locus_tag="ACGEXS_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015977653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HFF8011244.1"
                     /translation="MKELPKSRLTFKESMIESQYLATKTKEEKKQYKQLSVEDKREIL
                     KEYQSKPRKEVKFESEINKSDENLSKIYQRFSEIGVEDLFGTKKEVKELPMILKDNEN
                     IMYVTSGLYNNNTYLIVCTDLRLLFLDKGMIYGLKFHEFPFEKINSVSYKKGLLFGEI
                     IIHHGSSSIAIGSISKNTVSRMAETIQEQISIRESSMKPSNSEKMSFSVADELIKYKE
                     LLDVGVISQEEFDKKKQQLLDID"
     gene            complement(1013..1687)
                     /locus_tag="ACGEXS_000196"
     CDS             complement(1013..1687)
                     /locus_tag="ACGEXS_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFF8011245.1"
                     /translation="MKLNYEKSFFKSAKAVYEITNGLYNLSFPLDIFEIISKDKRIKL
                     VTFSEFSQNTGTLYFKIPSIFGSEEAFHIRKGDKAIIVYNDLLPMNRLRFTLAHEYGH
                     FIMGHTGVNLNKTFTYKDYYRRIAEEYEANSFASCLLFPLHIRYKYINNFNIEQISYK
                     YQMSFQAIHIAVKVIRRHIHNGLNDYMSNNENYHAENYLSFLEEKMESKSDFINEFKY
                     AYDLTI"
     gene            complement(1704..2036)
                     /locus_tag="ACGEXS_000197"
     CDS             complement(1704..2036)
                     /locus_tag="ACGEXS_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8011246.1"
                     /translation="MNLVQRIRNLCNSKGMTFAELERTLGFSNGQIRRWEKTKPGIDK
                     VQKIADHFDVSVDYLLGREKDEYSGEDKSEDILIMHRATENMTEAQRQKALTILEAMF
                     DDWDDLTK"
     gene            2299..2493
                     /locus_tag="ACGEXS_000198"
     CDS             2299..2493
                     /locus_tag="ACGEXS_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFF8011247.1"
                     /translation="MSTTDFGLKVRTELLKRNMTNKQLAEMLEISSAYLSDILRGRRD
                     AFEQKKRIAKILEIKEEVKS"
     gene            2493..3257
                     /locus_tag="ACGEXS_000199"
     CDS             2493..3257
                     /locus_tag="ACGEXS_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HFF8011248.1"
                     /translation="MNEIKTFSNDMFSILIKQDNENNLFDLETVAKSLGFTQFKNGKQ
                     YIRWETINKYLGKYLSQEVGKGDFIPEPMVYKLAFKAGNAVAEKFQDWLAMEVLPAIR
                     KHGIYATDNVIEQTLKDPDYIITVLTEYKKEKEQNLLLQQEIGELKPKADYVDEILKS
                     TGTLATTQIAADYGISAQKLNKLLHEARLQRKVNKQWVLYSEHMGKSYTDSDTITIVR
                     SDGREDTVLQTRWTQKGRLKIHEIMTEFGYEANVTA"
     gene            3274..3468
                     /locus_tag="ACGEXS_000200"
     CDS             3274..3468
                     /locus_tag="ACGEXS_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011249.1"
                     /translation="MQAQNKKVIYYYYDEAGNRRLLSIGNLDTYLLADIKSRFGLYKK
                     AIPDLDNLYIQIDGIEFKLY"
     gene            3499..3642
                     /locus_tag="ACGEXS_000201"
     CDS             3499..3642
                     /locus_tag="ACGEXS_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011250.1"
                     /translation="MLQKFRISKEKNKLKLKLLKHASYCLETSNNPELLRAVAELLIK
                     INR"
     gene            complement(3663..4220)
                     /locus_tag="ACGEXS_000202"
     CDS             complement(3663..4220)
                     /locus_tag="ACGEXS_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011251.1"
                     /translation="MECLLELLKMSIPLIAVIFSWWLATKTANEKFEEQIKKETYDNF
                     YSEILKACYELPSTDLLDFMSFYSYHRKDKISEIIIKNFSYVPPDIVNYWKKYNLALK
                     FFEHDYEKDVQAREVLSRFLNYYSHLIIIKSLEESEKLSEELKLPQLSTQLLSPLKRI
                     DFYKSQLPSQKLILKYHLQELVPNS"
     gene            4291..4512
                     /locus_tag="ACGEXS_000203"
     CDS             4291..4512
                     /locus_tag="ACGEXS_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011252.1"
                     /translation="MMKNSLQAKELAVILSVSKSKAGQIIRELNKELEDEGYIAIRGR
                     IPVQLARKKFPYHDLSDQRIIEELKKVNE"
     gene            4505..4666
                     /locus_tag="ACGEXS_000204"
     CDS             4505..4666
                     /locus_tag="ACGEXS_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 family protein"
                     /protein_id="HFF8011253.1"
                     /translation="MSKTYKSYLLAVLCFTVLAIVLMPLLYFTTAWSIAGFASIATFI
                     FYKEYFYEE"
     gene            4758..5018
                     /locus_tag="ACGEXS_000205"
     CDS             4758..5018
                     /locus_tag="ACGEXS_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1108 family protein"
                     /protein_id="HFF8011254.1"
                     /translation="MYYEIGEIIRKNIHVNGFDFKLFILKGHMGISIQVKDMNNVPIK
                     HAYVVDENDLDMASDLFNQAIDEWIEENTDEQDRLINLVMKW"
     gene            5028..5249
                     /locus_tag="ACGEXS_000206"
     CDS             5028..5249
                     /locus_tag="ACGEXS_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2483 family protein"
                     /protein_id="HFF8011255.1"
                     /translation="MKQTVTYIIRHRDMPIYITNKPTDNNSDVSYSTNRNRAREFNGM
                     EEASINMDYHKAIKKTVTETIEYEEVEHD"
     gene            5242..5865
                     /locus_tag="ACGEXS_000207"
     CDS             5242..5865
                     /locus_tag="ACGEXS_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFF8011256.1"
                     /translation="MTEQTLFEQLNSKNVNDHTEQKNGLTYLAWSYAHQELKKIDPNY
                     TVKVHEFPHPDINTENYFVPYLATPEGYFVQVSVTVKDSTETEWLPVLDFRNKSLAKG
                     SATTFDINKAQKRCFVKASALHGLGLYIYNGEELPSASDNDITELEERINQFVNLSQE
                     KGRDATIDKTMRWLKISNINKLSQKQIAEAHQKLDAGLKQLDSEEKQ"
     gene            5865..6293
                     /gene="ssb"
                     /locus_tag="ACGEXS_000208"
     CDS             5865..6293
                     /gene="ssb"
                     /locus_tag="ACGEXS_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFF8011257.1"
                     /translation="MLNRAVLVGRLTKDPELRSAPNGVNVGTFTLAVNRTFTNAQGER
                     EADFINVVVFKKQAENVKNYLSKGSLAGVDGRLQTRSYENKVGQRVFVTEVVADSVQF
                     LEPKNNNQQPNNNYHQQRQTQTGNNPFDNTTAITDDDLPF"
     gene            6307..6981
                     /locus_tag="ACGEXS_000209"
     CDS             6307..6981
                     /locus_tag="ACGEXS_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFF8011258.1"
                     /translation="MPKITSYITQDDGTTTVVISDVELGNKETLLLDNGFDVEVDVNV
                     IDPFQITGKQRRKIFALVKDIEEHTGQPMDYMRHMFIEFVRTYYGYDERISLSNCTRT
                     QASQIIEATLDWTFYNDIPLSYKTSNLLKQDKSFLYWSTVNRNCVICGKPHADLAHYE
                     AVGRGMNRNKMNHYDKHVLALCREHHNEQHAIGVKSFDDKYQLHDSWIKVDERLNKML
                     KGEKKE"
     gene            6978..7127
                     /locus_tag="ACGEXS_000210"
     CDS             6978..7127
                     /locus_tag="ACGEXS_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011259.1"
                     /translation="MNRLRIIKIALLIVILAEEIRSAKKIKKFTPEDSKGFPDITKDS
                     IKEPK"
     gene            complement(7120..7401)
                     /locus_tag="ACGEXS_000211"
     CDS             complement(7120..7401)
                     /locus_tag="ACGEXS_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011260.1"
                     /translation="MEKPYMLTYDLNSPGQKYEELRNVIKKEISNGHCNYWKSSFLFR
                     SSLSTSEMIEKLKPYLDSGDKLFVTEIVNNKQGWLTKEQWDFINHNIFI"
     gene            7467..8237
                     /locus_tag="ACGEXS_000212"
     CDS             7467..8237
                     /locus_tag="ACGEXS_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HFF8011261.1"
                     /translation="MATFRVYKESGNFVTVHKDFIHDSNISWKAKGILLYLLSRPDNW
                     QIYETELEQHSTDGLSGLKSGIKELEEIGYIQRSRKRDKSGRLNGYEYLVYEQPHHIR
                     FSNVGKTVNGKTNNGKTVNGKSHTTNNNSTNNDLTNNNNTNNEGSILSGNPTVSSIPY
                     KEIIEYLNKKAGKHFKHNTAKTKDFIKARWNQDFRLEDFKKVIDIKTAEWLNTDSDKY
                     LRPETLFGSKFEGYLNQKIQPTGTNQLERMKYDESYWD"
     gene            8247..9026
                     /locus_tag="ACGEXS_000213"
     CDS             8247..9026
                     /locus_tag="ACGEXS_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8011262.1"
                     /translation="MKPLFSEKINESLKKYQPTHVEKGLKCERCGSEYDLYKFAPTKK
                     HPNGYEYKDGCKCEIYEEYKRNKQRKINNIFNQSNVNPSLRDATVKNYKPQNEKQVHA
                     KQTAIEYVQGFSTKEPKSLILQGSYGTGKSHLAYAIAKAVKAKGHTVAFMHIPMLMDR
                     IKATYNKNAVETTDELVRLLSDIDLLVLDDMGVENTEHTLNKLFSIVDNRVGKNNIFT
                     TNFSDKELNQNMNWQRINSRMKHNARKVRVIGDDFRERDAW"
     gene            9020..9178
                     /locus_tag="ACGEXS_000214"
     CDS             9020..9178
                     /locus_tag="ACGEXS_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011263.1"
                     /translation="MVTKEFLKIKLECSDMYAQKLIDEAQGDENKLYDLFIQKLAERH
                     TRPAIVEY"
     gene            9191..9412
                     /locus_tag="ACGEXS_000215"
     CDS             9191..9412
                     /locus_tag="ACGEXS_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3269 family protein"
                     /protein_id="HFF8011264.1"
                     /translation="MPKEKYYLYREDGTEDIKVIKYKENENEVYSLTGAHFSDEKKIM
                     TDSDLKRFKGAHGLLYEQELGLQATIFDI"
     gene            9422..9826
                     /locus_tag="ACGEXS_000216"
     CDS             9422..9826
                     /locus_tag="ACGEXS_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFF8011265.1"
                     /translation="MSKYNAKKVEYKGIVFDSKVECEYYQYLESNMNGTNYDRIEIQP
                     KFELQPKFGKQRPITYIADFSLWKDGKLVEVLDVKGKATEVANIKAKIFRYQYRDVNL
                     TWICKAPKYTGQEWMVYEDLVKVRRKRKREMK"
     gene            9831..10016
                     /locus_tag="ACGEXS_000217"
     CDS             9831..10016
                     /locus_tag="ACGEXS_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3113 family protein"
                     /protein_id="HFF8011266.1"
                     /translation="MQQQAYINATIDIRIPTEVEYQHFDDVDDEKDALADYLYNNPDE
                     ILEYDNLKIRNVNVEVE"
     gene            10017..10388
                     /locus_tag="ACGEXS_000218"
     CDS             10017..10388
                     /locus_tag="ACGEXS_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001651750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SA1788 family PVL leukocidin-associated protein"
                     /protein_id="HFF8011267.1"
                     /translation="MAKTARIVRIHDKPYRFSKFEMELIESHGITPGMVSKRVKDGWE
                     LHEAMDAPEGTRLSEYREKKTIERLEQARLERKLERERKREAELRRKKPHLFNVPQKH
                     SRDPYWFDVTYNQMFKKWSEA"
     gene            10388..10642
                     /locus_tag="ACGEXS_000219"
     CDS             10388..10642
                     /locus_tag="ACGEXS_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HFF8011268.1"
                     /translation="MSIISNRKVDMNEIQDNVKQPAHYTYGDIEIIDFIEQVTAQYPP
                     QLAFAIGNAIKYLSRAPLKNGHEDLAKAKFYVDRVFDLWE"
     gene            10642..10890
                     /locus_tag="ACGEXS_000220"
     CDS             10642..10890
                     /locus_tag="ACGEXS_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAV1978 family virulence-associated passenger
                     protein"
                     /protein_id="HFF8011269.1"
                     /translation="MTMTDSGRKEYLKHFFGSKRYLYQDNERVAHIHVVNGTYYFHGH
                     IVPGWQGVKKTFDTAEELETYIKQSDLEYEEQKQLTLF"
     gene            10904..11110
                     /locus_tag="ACGEXS_000221"
     CDS             10904..11110
                     /locus_tag="ACGEXS_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011270.1"
                     /translation="MKIKIEKEMNLPELIQWAWDNPKLSGNKRFYSNDVERNCFVTFH
                     VDSILCNVTGYVSINDKFTVQEEI"
     gene            11113..11517
                     /locus_tag="ACGEXS_000222"
     CDS             11113..11517
                     /locus_tag="ACGEXS_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011271.1"
                     /translation="MKIKVKKEMRLDELIKWARENPDLSQGKIFFSTGFSDGFVRFHP
                     NTNKCSTSSFIPIDIPFIVDIEKEVTEETKFDRLLEVYEIQEGVYKSALHKGISLNER
                     FEDDNIFPTKAFYILNDDMTMTLIWKDGELVE"
     gene            11514..11930
                     /locus_tag="ACGEXS_000223"
     CDS             11514..11930
                     /locus_tag="ACGEXS_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFF8011272.1"
                     /translation="MTLRFRAWDKLGKEMHKVSAIDFSSKGARIIRLAGVQSNGKENH
                     KRWHSSVELMQYTGVKDVNGVEIFEGDIVKVNVLEGIGPNASIVFKYGMFGVENDMHG
                     YGYDKGLYPLNLIINRHEVEVIGNIFESSHLLEVTE"
     gene            11927..12292
                     /locus_tag="ACGEXS_000224"
     CDS             11927..12292
                     /locus_tag="ACGEXS_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFF8011273.1"
                     /translation="MNYETGFQLGVMEARLKKMRKQRDEYKKQRDELIVDIAKLRERN
                     KELENMWRTLKNELLGRYEHYCFKYRELHPESKANRIGAIYIGGKGTADIILSRMEEL
                     DGTNEFNEFLDRLEDDTNE"
     gene            12285..12539
                     /locus_tag="ACGEXS_000225"
     CDS             12285..12539
                     /locus_tag="ACGEXS_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HFF8011274.1"
                     /translation="MNNREQIEQSVISASAYNGNDTEGLLKEIEDVYKKARAFDEILE
                     GMTNAIQHSVKEGIELDEAVGIMAGQVVYKYEEAQENEEI"
     gene            12526..12696
                     /locus_tag="ACGEXS_000226"
     CDS             12526..12696
                     /locus_tag="ACGEXS_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015978317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011275.1"
                     /translation="MKKFNVQITYTGMIEETIEAESLEEAEFEADVTARLEAPFDCDE
                     YEINVEEAQEND"
     gene            12689..13225
                     /locus_tag="ACGEXS_000227"
     CDS             12689..13225
                     /locus_tag="ACGEXS_000227"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFF8011276.1"
                     /translation="MTNILTTDQLQELIQIQKEFDDRIPTRNLNDTVASMIIEFVEWI
                     NTLEFFKNWKKQPGKPLDTQLDEIADYLAFSLQLTLTIVDEEDLEETTEVMVDLIENE
                     VTLPKLHSVYFVHVLHTLTEQFVKGIDNSIVQVLIMPFLYANTYYSIDQLIEAYKKKM
                     KRNHERQDGTADAGKGYV"
     gene            13262..13549
                     /locus_tag="ACGEXS_000228"
     CDS             13262..13549
                     /locus_tag="ACGEXS_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HFF8011277.1"
                     /translation="MTQYLITTFKDSTGRKHTHITKAKSNQRFTVVEAESKEEAKEKY
                     EARNAPVDGATNLNDIKSNIGIFHVEKVEPNEGMVAINIETMKPFEEADHD"
     gene            13542..13778
                     /locus_tag="ACGEXS_000229"
     CDS             13542..13778
                     /locus_tag="ACGEXS_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011278.1"
                     /translation="MINIPKMKFPKKYTEIIKKYKNKTPEEKSKIENDFIKDINDKDS
                     EFYSPMMANMNEHELRAMLRMMPSLIDTGDDNDD"
     gene            13768..14157
                     /locus_tag="ACGEXS_000230"
     CDS             13768..14157
                     /locus_tag="ACGEXS_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011279.1"
                     /translation="MMIKKLKNMGWFDIFIAGILSLFGIFALLLVVAFPIYTVTSYQH
                     KETHQGTITDKYNKRQDKEDKFYIVLDNKQVIENSDLLFKKKFDSADIQARLKVGDKV
                     EVNTIGYRIHFLNLYPVLYEVKKVDKQ"
     gene            14154..14327
                     /locus_tag="ACGEXS_000231"
     CDS             14154..14327
                     /locus_tag="ACGEXS_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator RinB"
                     /protein_id="HFF8011280.1"
                     /translation="MIKQISRLLFLLAMYELGKYVTEQVYIMMTANDDVEAPSDFAKL
                     SDHSDLMRAEVSE"
     gene            14328..14729
                     /locus_tag="ACGEXS_000232"
     CDS             14328..14729
                     /locus_tag="ACGEXS_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011281.1"
                     /translation="MYSKESIVNMIGTHKMKCNVLADVIPEYDSNSIAQYGIQATLPK
                     PQGENSSKVEDVVVRLERANKRYAQMLKEVEFINQSQQRLGHVDFCFLELLKKGYNRD
                     AIIKKMPNSKLNRNNFLARRDELAEKIYLLQ"
     gene            15084..15578
                     /locus_tag="ACGEXS_000233"
     CDS             15084..15578
                     /locus_tag="ACGEXS_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051276 - chromosome organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFF8011282.1"
                     /translation="MIKLTPKQEKFVLGLIEGKSQRKAYIDAGYSTKGKSGEYLDKEA
                     STLFKNRKVSGRYEKLRQEVAEQSKWTRQKAFEEYEWLKNVAKNDIEIEGVKKATADA
                     FLASLDGMNRMTLGNEVLANKKIETEIKMLEKKIEQIDKGDSGTEDKIKQLHDAITEV
                     IVNE"
     gene            15571..16794
                     /locus_tag="ACGEXS_000234"
     CDS             15571..16794
                     /locus_tag="ACGEXS_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFF8011283.1"
                     /translation="MNKLKSLYTDKQIEILKQTQKQDWFMLINHGAKRTGKTILNNDL
                     FLRELMRVRKIADEEGIETPQYILAGATLGTIQKNVLIELTNKYGIEFNFDKYNSFML
                     FGVQVVQTGHSKVSGIGAIRGMTSFGAYINEASLAHEEVFDEIKSRCSGTGARILVDT
                     NPDHPEHWLLKDYIENTDPKAGILSHQFKLDDNNFLNDRYKESIKASTPSGMFYERNI
                     NGMWVSGDGVVYADFDLNENTIKADELDDIPIKEYFAGVDWGYEHYGSIVLIGRGIDG
                     NFYFIEEHAHQFKFIDDWVVIAKDIVSRYGNINFYCDTARPEYITEFRRHRLRAINAD
                     KSKLSGVEEVAKLFKQNKLLVLYDNMDRFKQEVFKYVWHPTNGEPIKEFDDVLDSLRY
                     AIYTHTKPERLRRGK"
     gene            16791..18215
                     /locus_tag="ACGEXS_000235"
     CDS             16791..18215
                     /locus_tag="ACGEXS_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016528746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046798 - viral portal complex [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0051027 - DNA transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFF8011284.1"
                     /translation="MTLYKLIDDIEAQGILPKHIEALIESHKDDRERMVNLYNRYKTH
                     IDYVPIFKRRPIEEKEDFETGGNVRRLDVSVNNKLNNSFDSEIVDTRVGYLHGVPVTY
                     DLDENAEKNEKLKKFITNFAIRNSVDDEDSEIGKMAAICGYGARLAYIDTNGDIRIKN
                     IDPYNVIFVGDNILEPTYSLRYFYEKDDDNGTDYVYAEFYDNAYYYVFRGEGIDALQE
                     VGRYEHLFDYNPLFGVPNNKEMIGDAEKVIHLIDAYDLTMSDASSEISQTRLAYLVLR
                     GMGMSEEMIQETQKSGAFELFDKDMDVKYLTKDVNDTMIENHLDRIEKNIMRFAKSVN
                     FNSDEFNGNVPIIGMKLKLMALENKCMTFERKMTAMLRYQFKVILSALKRKGYNLDDD
                     SYLNLIFKFTRNIPVNKLEESQVLINLKGQVSERTRLGQSQLVDDVDYELDEMEKESL
                     EFNDKLPDIDEGDANDKSQNNQSE"
     gene            18184..19137
                     /locus_tag="ACGEXS_000236"
     CDS             18184..19137
                     /locus_tag="ACGEXS_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HFF8011285.1"
                     /translation="MTNPKITNQNDINEYIEGLISKAEKPIEQLFANRLKEIKQIIAD
                     MFEKYQNDDVYVTWTEFNKYNRLNKELTRIGTMLTDDYRQVAKMIQKSQEDAYIEKFL
                     MSLYLYEMASQTSMQFDVPSKEVIKSAIEQPIEFIRLMPTLQKHRDEVLKKIRMHITQ
                     GIMSGEGYSKIAKAIRDDIGMSKAQSLRVARTEAGRAMSQAGLDSAMVAKDNGLKMKK
                     RWHATKDTRTRDTHRHLDGESVEIDQNFKSSGCVGQAPKLFIGVNSAKENINCRCKLL
                     YYIDENELPTVMRARKDDGKNEVIPFMTYREWEKYKRKGGN"
     gene            19139..19345
                     /locus_tag="ACGEXS_000237"
     CDS             19139..19345
                     /locus_tag="ACGEXS_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011286.1"
                     /translation="MDFKIKVNVDTGEAIEKLERIKSLYEEIIELQNEKVVVNVTVKN
                     EADLDMVKTSISEENAKNNDFTLF"
     gene            19448..20032
                     /locus_tag="ACGEXS_000238"
     CDS             19448..20032
                     /locus_tag="ACGEXS_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFF8011287.1"
                     /translation="MNIEEVKSFFEEHKDDKEVKDYLNGLKTVSVDDVKGFLDTEEGK
                     RFIQPELDRYHSKGLESWKEKNLEDLIEQEVRKRNPEQSEEQKRISALEQELEKRDAE
                     AKREKLRSNALGKAQELNLPTSLVDRFLGDSDEDTEQNLKALKETFDKYVQKGVESKF
                     KSSGRDVKESQNQDLDPSNVKSIEEMAKEINIRK"
     gene            20049..20963
                     /locus_tag="ACGEXS_000239"
     CDS             20049..20963
                     /locus_tag="ACGEXS_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000235170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFF8011288.1"
                     /translation="MATPTYTPGNVILSDFKNGVIPAEQGTLIMKDIMANSAIMKLAK
                     NEPMTAQKKKFTYLAKGVGAYWVSETERIQTSKPEYAQAEMEAKKIGVIIPLSKEFLK
                     WTAKDFFNEVKPLIAEAFYKAFDQAVIFGTKSPYNTSTSGKPLVEGAEEKGNVVTDTN
                     NLYVDLSALMATIEDEELDPNGVLTTRSFRSKMRNALDANDRPLFDANGNEIMGLPLS
                     YTGADVYDKKKSLALMGDWDYARYGILQGIEYAISEDATLTTLQASDASGQPVSLFER
                     DMFALRATMHIAYMNVKPEAFATLKPTE"
     gene            20975..21118
                     /locus_tag="ACGEXS_000240"
     CDS             20975..21118
                     /locus_tag="ACGEXS_000240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011289.1"
                     /translation="MANPAEEIKVKKDNMTITVTKKAFDSYYSLVGYKEVKSRRTTSD
                     KSE"
     gene            21124..21474
                     /locus_tag="ACGEXS_000241"
     CDS             21124..21474
                     /locus_tag="ACGEXS_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016528745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HFF8011290.1"
                     /translation="MTLYEDVKLLLKKNGVEVKSDEEEIFKMEVDGILEDVRDITNND
                     FMKDGQVIYPYSIKKYVADVLEYYQRPEVKKNLKSRSMGTVSYTYNDGVPDYISGVLN
                     RYKRAKFHPFKPIR"
     gene            21486..21821
                     /locus_tag="ACGEXS_000242"
     CDS             21486..21821
                     /locus_tag="ACGEXS_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFF8011291.1"
                     /translation="MFNPYDEFPHTISIGSIKKVGEYPIIQERFVSDKTIKGFMDTPT
                     TSEQLKFHQMSQEYDRNLYVPYDLPISKNNLFEYEGRIFSIEGDSVDQGGQHEIKLLR
                     LKQVPYGKS"
     gene            21808..22215
                     /locus_tag="ACGEXS_000243"
     CDS             21808..22215
                     /locus_tag="ACGEXS_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002480803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFF8011292.1"
                     /translation="MAKVKYGADSMVVELDKFDKKIEEWVKKGIAKTTMKIYNTAVAL
                     APADLGFLKESIDFRFENNGLTGVINVGAEYSVYVEYGTGIYATKGSRAKKIPWSYKD
                     ANGKWHTTKGQAPQPFWNPAIDAGRKTFEQYFS"
     gene            22228..22653
                     /locus_tag="ACGEXS_000244"
     CDS             22228..22653
                     /locus_tag="ACGEXS_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFF8011293.1"
                     /translation="MWVSVEPELTNQIYKRLISDPNINKIVGDRVFDVVQDDAVYPYI
                     VVGESNVTNNESSATMRETVGIVIHVYSQFATQYEAKLILSAIGYVLNRTIEIENYEF
                     QYSCIDSQAVFPDIDRFTKHGTIRLLFKYRHKKKNEGVY"
     gene            22654..23211
                     /locus_tag="ACGEXS_000245"
     CDS             22654..23211
                     /locus_tag="ACGEXS_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFF8011294.1"
                     /translation="MAQKNYLAVVRPAETDLDPVESLLLADLQEGGHTIENDLAEIVR
                     GGKTDYSPNAMSESFKLTIGNVPGDKGIEAVKHAVQTGGQLRIWLYERNKRADGKHHG
                     MFGYVVPESFEMSFDDESDKIELSLKVKWNTAEGAEDNLPKEWFEAAGAPTVEYEKFG
                     EKVGTFENQKKASVVSDSHTEDHSM"
     gene            23278..23784
                     /locus_tag="ACGEXS_000246"
     CDS             23278..23784
                     /locus_tag="ACGEXS_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HFF8011295.1"
                     /translation="MTEFNPITTLKINDGEKDYEVEAKVTFAFDRKAEKFSEDSEDGR
                     KGAMPGFNVIFNGLLESRNKAILQFWECATAYLKNPPTREQLEKAIDDFITENEDTLP
                     LLQGALDKLNNSGFFKRESRSYWMTLNKAPNMAKSEDKEMTKAGIEMMKENYKEIMGA
                     EPYTITQK"
     gene            23829..24113
                     /locus_tag="ACGEXS_000247"
     CDS             23829..24113
                     /locus_tag="ACGEXS_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011296.1"
                     /translation="MLALTPAEWRDWLIGGQDRYLDQRQLLIEQAQANGLVQASKRLT
                     SMIRDIEKQRYEIREPGSYARVQKVRLEEEKRRRELFKEGTRKFLESKGG"
     gene            24117..27002
                     /locus_tag="ACGEXS_000248"
     CDS             24117..27002
                     /locus_tag="ACGEXS_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFF8011297.1"
                     /translation="MDTHFMAKIMANIRDFQSNVRKAQRLAKTSVPNEIETDVKADIS
                     RFQRALQRAKAMAQKWREHNVKIDGNNSPLKRAIASAKTMLATLHNKTIKVNFDTRGM
                     TKTQILTKALNQSLTDYSEKMDALATKIRTFGTIFAQQVKGLMIASIQALIPVIAGLV
                     PAIMAVLNAVGVLGGGVLGLVGAFSVAGLGVVGFGAMAISALKMVEDGTLAVTKEVQN
                     FRDASDQLKTTWRDIVKENQASIFNAMSAGIRGVTSAMSQLKPFLSEVSMLVEANARK
                     FEDWVKHSETTKKAFEALNSIGGAIFGDLLNAAGRFGDGLINIFTQLMPLFKFVSQGL
                     QNMSIAFQNWANSVAGQNAIKAFIDYTTTNLPKIGQIFGNVFAGIGNLMIAFAQNSSN
                     IFDWLVKLTSQFRAWSEQVGQSQGFKDFISYVQENGPTIMQLIGNIVKALVAFGTAMA
                     PIASKLLDFITNLAGFIAKLFEAHPAVAQIIGVIGILGGVFWALMAPIAAVSSVLSNV
                     FSMTLLNVVKRILDLTRITGVVSKAFGLLTGAFTSISWPILAVVAVIGVFIGILVYLW
                     KTNENFRKTITEAWNGIKTAVSGAIQGVVDWLTQLWGKIQSTLQPIMPILQVLGQVFM
                     QVLGVLVIGIITNVMNIIQGLWTLITIAFQAIGTVISVAVQIIVGLFTALIQLLTGDF
                     SGAWETIKTTVTNVLDTIWQYMQSVWESIIGFLTGVMNRTLSMFGTSWSQIWSTITNF
                     VSSIWNTVTSWFSRVAWSVAEKMGQALNFIITKGSEWVSNIWNTVTSFASKVADGFKR
                     VVSNVGDGMSDALGKIKSFFSDFLNAGAELIGKVAEGVANAAHKVVSAVGDAISSAWD
                     SVTSFVSGHGGGSSLGKGLAVSQAKVIATDFGSAFNKELSSTLTDSIVDPVSTSIDRH
                     MTSDVQHSLKENNRPIVNVTIRNEGDLDLIKSRIDDMNAIDGSFNLL"
     gene            27017..27958
                     /locus_tag="ACGEXS_000249"
     CDS             27017..27958
                     /locus_tag="ACGEXS_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail domain-containing protein"
                     /protein_id="HFF8011298.1"
                     /translation="MIAHDIEVIRNGSQYRVSDNPFTYNHLEVVEYNVTGAGYHRNYS
                     DIEGIDGRFHNYAKEELKKVELKLRYKVPKIAYASHLKSDVQALFAGRFYLRELATPD
                     NSIKYEHILDIPKDKQAFELDYVDGRQLFVGLVSEVSFDTTQTSGEFSLSFETTELPY
                     FESVGYSTDLESDNDPEKWSVPDRLPTNEGDKRRQMTFHNTNSGEVYYNGDVPLTQFN
                     QFNVVEIELAEDVKANDKDGFTFYTDKGNISVIKEVDLKAGDKIIFDGKHTYRGYLNI
                     DSFNKTLEQPVLYPGWNRFKSNKVMKQITFRHKLYFR"
     gene            27969..29855
                     /locus_tag="ACGEXS_000250"
     CDS             27969..29855
                     /locus_tag="ACGEXS_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFF8011299.1"
                     /translation="MPILLKSLQGVGHAINVSTKISKKLNEDSSLDLTIIENASTFDA
                     IGAITKMWTITHVEGEDDFNEYVIVILDKSTIGEKIRLDIKARQKELDDLNNSRIYQE
                     YNESFTGVEFFNTVFKGTSYKYVLHTKVDASKFEGLGKGDTRLEIFKKGLERYHLEYE
                     YEAKTKTFHLYDELSKVADYYIKSGVNADNVKIQEDASKCYTYIRGYGDFDGQQTYTE
                     AGLQFEFTHPLAQLIGKREAPPLIDGRIKKEDILKKSMELVIKKSVTASISLDFVAQP
                     EHFPEANPRIGDVVRVVDSAIGYNDLVRIVEITTHRDAYNNITKQDVVLGDFTRRNRY
                     NKAVHDAANYVKSVKSTKSDPSKELKALNAKVNASLSINNELVKQNEKINAKVDKMNT
                     KTVTTANGTIMYDFTSQSSIRNIKSIGTIGDSVARGSHAKTNFTEMLGKKLKAKTTNL
                     ARGGATMATVPIGKEAVENSIYRQAEQIRGDLIILQGTDDDWLHGYWAGVPIGTDKTD
                     TKTFYGAFCSAIEVIRKNNPDSKILVMTATRQCPMSGTTIRRKDTDKNKLGLTLEDYV
                     NTQILACSELDVPVFDAYHTDYFKPYNPAFRKASMEDGLHPNEKGHEVIMYELIKDYY
                     SFYD"
     gene            29868..31766
                     /locus_tag="ACGEXS_000251"
     CDS             29868..31766
                     /locus_tag="ACGEXS_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011300.1"
                     /translation="MAYGLITSLHSMTGRKIVAQHEYNYRLLDEGMSKLEKMFIYHQK
                     EEIYAHSAKQIKYLNDSVEDYLTYLNGRFSNMILGHNGDGINEVKDARIDNTGYGHKT
                     LQDRLYHDYSTLDAFTKKVEKAVDEHYKEYQATEYRFEPKEQEPEFITDLSPYTNAVM
                     QSFWVDPRTKIIYMTQARPGNHYMLSRLKPNGQFIDRLLVKNGGHGTHNAYRYIDGEL
                     WIYSAVLDSNKNNKFVRFQYRTGEITYGNEMQDVMPNIFNDRYTSAIYNPIENLMIFR
                     REYKASERQAKNSLNFIEVRSADDIDKGIDKVLYQMDIPMEYTSDTQPMQGITYDAGI
                     LYWYTGDSNTANPNYLQGFDIKTKELLFKRRIDIGGVNNNFKGDFQEAEGLDMYYDLE
                     TGRKALLIGVTIGPGNNRHHSIYSIGQRGVNQFLKNIAPQVSMTDSGGRVKPLPIQNP
                     AYLSDITEVGHYYIYTQDTQNALDFPLPKAFRDAGWFLDVLPGHYNGALRQVLTRNST
                     GRNMLKFERVIDIFNKKNNGAWNFCPQNAGYWEHIPKSITKLSDLKIVGLDFYITTEE
                     SKRFTDFPKDFKGIAGWILEVKSNTPGNTTQVLRRNNFPSAHQFLVRNFGTGGVGKWS
                     LFEGKVVE"
     gene            31766..33589
                     /locus_tag="ACGEXS_000252"
     CDS             31766..33589
                     /locus_tag="ACGEXS_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFF8011301.1"
                     /translation="MIVDNFSKDDNLIELQTTSQYNPIIDTNISFYESDRGTGVLNFA
                     VTKNNKPLSISKHNAMTSIVLKTDNFDDEHGAYISDELTIVDAINGRMQYVIPNEFLK
                     YTGRVHAQAYFTQNGSNNVIVERQFNFNIQNDLISNFDGKTKLVYIKSIQDLTESVKE
                     EVEDLKKSLSDTKSLVTEIDSRINQGIQRLEIKQNEAVQMITTTQDKAVQYINSEFQK
                     IVDKEQAIFERVNEVEQQINGADLVKGNSTTNWQKSKLTDDYGKAIESSEQSIEAVLR
                     HANSSMIIHITNAKDAPEKADIGTLEKPGQDGVDDGSSFDESTYTSSKSGVLVVYVVD
                     NNTARATWYPDDSNDEYTKYKIYGTWYPFYKKNDGNLTKQFVEEISNNTLNQAKQYVD
                     GKLQSISWQQHKLTEHNGQSIQKNLYNAKGNLEALGAGNYYVTSVPDLPGIVESYEGY
                     LSVFVKDDANKLFNFTPLNSKKVYTRSITNGRLDSQWATPNEHKTAVLFDGAANGVGT
                     RINLTEAYTNYAILFISGTYPGGVIEAFSLTSIPNAIQLSKTNVVDSDGNGGGSYECL
                     ITKESGTTLKIDNDVYLDLGSKTGSGANANRVTINKIVGWK"
     gene            33589..33966
                     /locus_tag="ACGEXS_000253"
     CDS             33589..33966
                     /locus_tag="ACGEXS_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2977 domain-containing protein"
                     /protein_id="HFF8011302.1"
                     /translation="MKITVNDKNEVIGYVNTGGLRNSLDVDDNNVPIKFKEEFEPRKF
                     VFTNGEIKYNSNFEKEDVPNASNQQSESDLSDEELRGMVASMQMQVTQVNILAMELKQ
                     QNAMLTQQLTELKAGKTNTEEDV"
     gene            33976..34149
                     /locus_tag="ACGEXS_000254"
     CDS             33976..34149
                     /locus_tag="ACGEXS_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFF8011303.1"
                     /translation="MKMIYPTFKDIKTFYVWGCYKNEQIKWYVDMGVIDKEEYALITG
                     EKYPEAKDEKSQV"
     gene            34190..34489
                     /locus_tag="ACGEXS_000255"
     CDS             34190..34489
                     /locus_tag="ACGEXS_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2951 domain-containing protein"
                     /protein_id="HFF8011304.1"
                     /translation="MFGFTKRHEHEWRIRRLEENDKTMLSTLNEIKLGQKTQEQVNIK
                     LDKTLDAIQRERQIDEKNKKENDKNIRDMKMWILGLIGTIFSTIVIALLRTIFGI"
     gene            34626..36500
                     /locus_tag="ACGEXS_000256"
     CDS             34626..36500
                     /locus_tag="ACGEXS_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFF8011305.1"
                     /translation="MGLPNPKTRKPTASEVVEWAKSNIGKRINIDNYRGSQCWDTPNF
                     IFKRYWGFVTWGNAKDMANYRYPKGFRFYRYSSGFVPEPGDIAVWHPGNGIGSDGHTA
                     IVVGPSNKSYFYSVDQNWVNSNSWTGSPGSLVRHPYVSVTGFVRPPYSKDTSKPSSTD
                     TSSASKANDSTITGEAKKPQFKEVKTVKYTAYSNVLDKEEHFIDHIVVMGDERSDIQG
                     LYIKESMHMRSVDELYTQRNKFISDYEIPHLYVDREATWLARPTNFDDPRHPNWLVIE
                     VCGGQTDSKRQFLMNQIQALIRGVWLLSGTDKELSETTLKVDPNIWRSMKDLINYDLI
                     KQGIPDDAKYEQVKKKMLETYIKRDILKRENIKEVTTQTTIRISDKTSVDSASTRGPT
                     ASDEKPSIVTEKSPFTFQQALDRQMSRGNPKKSHTWGWANATRAQTSSAMNVKRIWES
                     NTQCYQMLNLGKYQGVSVSALNKILKGKGTLDGQGKAFAEACKKNNINEIYLIAHAFL
                     ESGYGTSNFASGRYGAYNYFGIGAFDNDPDYAMKFAKNKGWTTPAKAIMGGASFVRKD
                     YINKGQNTLYRIRWNPKNPATHQYATAIEWCQHQASTIAKLYKQIGLKGIYFIRDKYK
                     "
     gene            36513..37751
                     /locus_tag="ACGEXS_000257"
     CDS             36513..37751
                     /locus_tag="ACGEXS_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFF8011306.1"
                     /translation="MYKIKDVETRIKNDGVDLGDIGCRFYTEDENTASIRIGINDKQG
                     RIDLKAHGLTPRLHLFMEDGSIFKNEPLIIDDVVKGFLTYKISKKVIKHAGYVRCKLF
                     LEKEEGKIHVANFSFNIVDSGIESAVAKEIDVKLVDDAITRILKDNATDLLSKDFKEK
                     IDKDVISYIEKNESRFIGAKGDKGEPGQPGAKGEAGKKGEQGAPGKNGTVVSINPDTK
                     MWQIDGKDTDIKAEPELLDKINIANVEGLENKLQEVEKIKDTTLNDSKTYTDSKIAEL
                     VDSAPESMNTLRELAEAIQNNSISESVLQQIGSKVSTEDFERFKQSLNSLYADKNHSH
                     TIKQIEGLENALSKKSDINHSHDERYLLSSNAFTKEEADKLYQPIGSSQPSLNIWTGS
                     ETEYNYLYQKDPNTLYLIKG"
     gene            37756..38151
                     /locus_tag="ACGEXS_000258"
     CDS             37756..38151
                     /locus_tag="ACGEXS_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011307.1"
                     /translation="MEGNFKNVKKLIYEGEEYTKVYAGNIQVWKKPSSFVIKPLPKNK
                     YPDSIEESTAKWTINGVEPNKSYQVTIENVRSGIMRISQTNLGSSELGISGVNSGVAS
                     KNINFSNPSGTLYVTISDVYSGSPTLTIE"
     gene            38207..38482
                     /locus_tag="ACGEXS_000259"
     CDS             38207..38482
                     /locus_tag="ACGEXS_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFF8011308.1"
                     /translation="MDIGTIVRTILLIVAWINQFLAIKHISPIPVDEVFISTVVTGIV
                     SIWTWWKNNNFTHASKKGQQKIYEVKAGIQSTGGAPKVNGDDNNAVG"
     gene            38469..39881
                     /locus_tag="ACGEXS_000260"
     CDS             38469..39881
                     /locus_tag="ACGEXS_000260"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015978294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFF8011309.1"
                     /translation="MPSVRTYSQAISYLKSLEGKAWNPDNAFGCQCFDTANQYWLYLF
                     NHRLKGVGAADIPTWNDFTNEATVYENTVSFQALPGDVVIFNRNYGGGYGHVGIVISA
                     TLDSITILEQNWLGGAYWSPPEVTTRRTHGYDFPMWFIRPFYAKETTANKLRSAVTPV
                     KQDKLSKGKKIMLVAGHGIGAYSNDPGAVANGENERDFNRKNIIPRVKKYLESVGNTV
                     LLYGGNSMNQDLYQDTLYGQRVGNYKDYGMYWIKSEVKPDAIIEFHLDSASPQASGGH
                     VIISDRFPADDIDKALSSALDKTVGKIRGVTPRGDLLNANVSADLNLNYRLIELGFIT
                     STKDLNYIKNNLDSFTKRIAEAINGRQIDAPSSKPSADKITWNWKGVFYPNPEKAIRV
                     RKTAGLTGTVVEEDSWLYTKDDWVKFDQVIKKDGYWWIRFKYQREGSSTNNFYCAVCR
                     ITDKEQKIKNEKYWGTIEWA"
     gene            complement(39941..40498)
                     /locus_tag="ACGEXS_000261"
     CDS             complement(39941..40498)
                     /locus_tag="ACGEXS_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015978304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4888 domain-containing protein"
                     /protein_id="HFF8011310.1"
                     /translation="MNKKLLTKTLIASALVLTTVGSGFHSSSNYNGINNVAKASEITD
                     SELWKNVRDALKDANIIDKTNNETIKVKYKLKNGGESEISGTANLDNLSNTNNSTVSP
                     DSVNRVDITRVNPNGNTIEANDAWKKLVDKLKEKHIVKVGDKVTIHSKDPSDEKVYGK
                     VGDQDSNVKNRLISPKDITHITIER"
BASE COUNT        14845 a         6153 c         8111 g        11404 t
ORIGIN      
        1 attatatatt tcttttaatt tactgtcatt tgagataaca acgatgcaag gttcttcaaa
       61 aaaagattga tttagataaa atatcgaaat cttgtaatcg tcttttctca tgaatgggaa
      121 ggcttccgga ttactactaa actgataaat gtatctgttt tcaactacat atttgtaacc
      181 ttctaaaaaa ttacgcaagt attcttttaa agttttattc tcttccatcc ctcatcctcc
      241 tcacgccaca caagcgctat taatcaatat ccaataattg ttgttttttc ttatcgaact
      301 cttcctgaga aattactccg acatctaata attctttata ttttattaat tcatcagcaa
      361 cagaaaaact cattttttca gaattggatg gtttcataga actttctcga atagagattt
      421 gttcttgtat tgtttccgcc attctagata cagtgttttt tgatatgctt cctatagcga
      481 tacttgatga accgtgatgt ataattattt cgccaaaaag aagtcctttt ttatacgaaa
      541 cagaattgat tttctcgaat ggaaattcat gaaatttcaa accatatatc atacctttat
      601 ctaagaataa caatcttaga tcagtacata ctattaagta ggtattatta ttgtacaatc
      661 ccgaagttac atacattatg ttttcattat cttttaaaat cataggtagt tctttcactt
      721 ctttttttgt accaaacaaa tcctctacac ctatttcgct aaatctttgg tagattttag
      781 ataagttttc gtcagattta ttgatttcac tttcaaattt cacttctttt ctaggtttac
      841 tttggtattc ttttaaaatt tctcttttgt cttcaacaga tagttgcttg tattgtttct
      901 tttcttcttt tgttttagtt gctaaatatt gactctcaat catactttct ttgaacgtta
      961 atctgctctt aggtaattct ttcatgttca tttctccttt attttttgat tgttaaatcg
     1021 ttagatcata agcatattta aattcattta taaaatcaga tttgctttcc attttctctt
     1081 ctaaaaaact taagtagttt tctgcgtggt aattttcgtt atttgacata tagtcgttta
     1141 acccattgtg tatatgtctt ctgattactt ttaccgctat atggatcgct tgaaaactca
     1201 tttgatactt gtacgaaatt tgctcaatat taaagttgtt tatatatttg tatcttatat
     1261 gtaaaggaaa caataaacat gaagcaaatg agtttgcttc atattcttca gcaatccttc
     1321 tataataatc tttatatgtg aatgttttat ttaaattaac tccagtatgt cccattataa
     1381 aatgaccata ttcatgagct aaagtaaatc ttagacgatt cataggcagt aaatcgttat
     1441 aaactataat cgctttgtct ccttttctaa tatgaaacgc ttcttctgaa ccgaaaatag
     1501 aaggtatttt aaaatataaa gtgccagtat tctgagaaaa ttcagagaaa gtcactaatt
     1561 taatacgttt atcttttgag ataatttcaa atatatctaa aggaaaagat aagttatata
     1621 gaccatttgt gatctcgtaa actgctttcg cagatttaaa aaaagatttt tcataattta
     1681 atttcaatta aaaagcccct ttgttactta gttaaatcat cccaatcatc aaacattgct
     1741 tctaatatag tcaaagcttt ttgcctttgt gcctccgtca tattttctgt agctcgatgc
     1801 ataataagaa tatcttcact tttatcttct ccggagtact catctttttc tctacctaat
     1861 aagtaatcaa ctgatacatc gaagtgatcg gcaatttttt gcaccttatc aatgcctggt
     1921 ttggttttct cccatcttct gatttgtccg tttgaaaacc ctaaagttct ctctaattca
     1981 gcaaaagtca taccttttga attgcacaaa ttacggattc tttgtactag attcataaat
     2041 ttctcctatc acagattaac tttttcgcta tttttgttga caattagcat aaaagttaat
     2101 atactgtatt taagctttaa atttagctta ctaaacacat aacaattatt cgttggggaa
     2161 cgagtattca atacctttat gacaggcatt acgaattgtt ataggtttat taaactatgc
     2221 ttaaatatta gcataaaagt tattggtgtt caacagataa tttatttgct tagaaaaaat
     2281 gttataggag gtgctaatat gtcgacaaca gatttcggct tgaaagtgag aacggaatta
     2341 ttaaaacgca acatgacaaa caagcaactt gcggaaatgc tagaaatttc aagtgcttac
     2401 ttatcggata ttttacgtgg acgtagagat gcttttgaac aaaagaaacg tattgcgaaa
     2461 attttagaaa ttaaagaaga ggtgaagagt taatgaatga aattaaaact ttcagtaacg
     2521 acatgttttc aatcttaatc aaacaagata atgaaaataa tttattcgat ttagaaactg
     2581 tcgcaaaaag tttggggttc actcagttta aaaacggcaa acaatatatt cgttgggaaa
     2641 ctatcaataa atatttaggt aaatatcttt cccaagaagt tgggaaaggc gatttcatac
     2701 cagaaccaat ggtatataag ttggctttca aagcaggtaa tgctgtagca gaaaaatttc
     2761 aagattggtt ggcgatggaa gtcctaccag ctattcgcaa acacggtatc tacgcaacag
     2821 acaatgtaat tgaacaaaca ttaaaagatc cagactacat cattacagtg ttgactgagt
     2881 ataagaaaga aaaagagcaa aacttacttt tacaacaaga aatcggagaa ctaaaaccca
     2941 aagcagacta tgtagatgaa atcttaaagt caactggcac attagccaca actcaaatcg
     3001 cggcagacta cggtatatca gcacaaaagt taaacaaact actacacgaa gctagactac
     3061 aacgaaaagt aaataaacag tgggtgcttt actcagaaca catgggcaag agttacacag
     3121 attcagacac tataacaatt gtgcgttctg atggcagaga agacacagtt ttacaaacta
     3181 gatggacaca aaaaggcaga ttgaaaatac atgaaatcat gactgaattc ggttatgaag
     3241 ctaacgtaac tgcttaacag gagggcgcag caaatgcaag ctcaaaacaa aaaagtcatt
     3301 tattactact atgacgaagc cggtaataga cgactattat caattggtaa tttagatacc
     3361 tatttattag cagatatcaa atcaagattt ggtttatata aaaaggcaat ccctgattta
     3421 gataatctat acattcaaat agatggtatc gaatttaaat tatattaaat ttttggaaat
     3481 gcaaaggagg cacaacaaat gttacaaaaa tttagaatct cgaaagaaaa aaataaatta
     3541 aaactcaaat tactaaagca tgctagttac tgtttggaaa caagtaacaa ccctgaactg
     3601 ttgcgagcag ttgcagagtt gcttataaag ataaatcgat aaatagcgaa ggagttgatt
     3661 ttttatgaat tcggaactaa ttcttgcagg tggtacttta ggattaattt ttgtgacggt
     3721 aattgagact tataaaagtc gatcctcttt aaaggagaaa gtaattgtgt cgatagctgc
     3781 ggtaatttca gttcttctga taatttctct gattcttcta acgatttaat aattatcagg
     3841 tgagaataat aatttaaaaa tcgacttaaa acttctctgg cttgtacatc tttttcataa
     3901 tcatgttcga aaaatttaag tgctagattg tatttcttcc aatagttaac tatatcggga
     3961 ggtacataac taaagttttt aatgattatt tcagaaatct tatctttgcg gtgataagag
     4021 taaaaactca tgaagtctaa taaatcggta gaaggcaact cataacaagc ttttagaatc
     4081 tcggagtaaa aattatcata ggtctctttt ttgatttgtt cttcgaattt ttcattagcc
     4141 gtttttgtag caagccacca agaaaaaatt accgctatta gaggtattga catttttaaa
     4201 agttctaata aacattccat aattatcact tcctttcact aggagataac taaattatac
     4261 acaacacaaa aataaaaagg aggaaaagat atgatgaaaa atagtttgca agctaaagaa
     4321 cttgcggtaa ttttatctgt ttctaaatcc aaagcaggac aaataataag agaactgaat
     4381 aaagagcttg aagacgaagg gtacattgcg atacgaggca gaatacccgt ccaattagct
     4441 aggaaaaaat tcccttatca cgacttatca gaccagagaa taatagagga gttgaagaaa
     4501 gtaaatgagt aaaacttata aaagctatct attagcagta ttgtgcttca cagtcttagc
     4561 gattgtgctt atgccattgc tgtacttcac tacagcatgg tcaattgcgg gattcgcaag
     4621 tatcgcaaca ttcatatttt ataaagaata cttttatgaa gaataaaaaa actgctactt
     4681 gcgccaacaa gtaacagtat caagcactta agaaaaattt caagttaaat ataaaatgaa
     4741 aaacggagga agtcaagatg tattacgaaa taggcgaaat catacgcaaa aatattcatg
     4801 ttaacggatt cgattttaag ctattcattt taaaaggtca tatgggcata tcaatacaag
     4861 ttaaagatat gaacaacgta ccaattaaac atgcttatgt cgtagatgag aatgacttag
     4921 atatggcatc agacttattc aaccaagcaa tagatgaatg gattgaagag aacacagacg
     4981 aacaggacag actaattaac ttagtcatga aatggtagga ggtcgctatg aagcagactg
     5041 taacttacat catccgtcat agggatatgc caatttatat aactaacaaa ccaactgata
     5101 ataattcaga tgttagttac tccacaaata gaaatagagc tagggaattt aacggtatgg
     5161 aagaagcgag tatcaatatg gattatcaca aagcaatcaa gaaaacagtg acagaaacta
     5221 ttgagtacga ggaggtagaa catgactgaa caaacattat ttgaacagtt gaacagtaaa
     5281 aacgtgaatg atcatacaga acaaaaaaat ggattaactt atctagcatg gtcatatgca
     5341 caccaagagc tgaaaaagat tgacccaaac tacacagtaa aagtacacga gtttccacat
     5401 ccagatatta acacagaaaa ttattttgta ccttatttgg ctacaccaga aggctatttt
     5461 gtacaggtat ctgtgactgt gaaagatagt acagagactg agtggcttcc agtattggac
     5521 tttagaaata aatcgcttgc taaaggtagt gcaacaactt tcgatattaa caaagcgcaa
     5581 aaacgatgtt ttgttaaagc ttcggcttta cacggtttag gcttatatat ctacaacggc
     5641 gaggaactac caagtgcaag tgacaacgat attacagaat tagaagagcg tatcaatcag
     5701 ttcgtgaact tatctcaaga aaaagggcga gatgcaacta tcgataaaac gatgagatgg
     5761 ctaaaaatat ctaacattaa taaattaagt caaaaacaaa tcgcagaagc acaccaaaaa
     5821 ttagatgcgg gattaaaaca attggatagt gaggagaaac aataatgtta aacagagcag
     5881 tattagtagg acgcttaaca aaagacccag aattaagaag cgcgccaaat ggcgtaaatg
     5941 taggtacatt cacattggca gtaaacagaa cattcacgaa tgctcaaggc gagcgtgaag
     6001 cagattttat aaacgtagta gtgttcaaga aacaagctga aaatgttaaa aactaccttt
     6061 ctaaagggtc gctggcaggt gtagacgggc gactacaaac acgtagctac gaaaataaag
     6121 tcgggcaacg tgtatttgtg acagaagtag tagcggacag tgttcaattc ttagaaccga
     6181 agaataacaa ccaacaacca aacaacaatt atcatcaaca aagacaaact caaactggta
     6241 ataatccttt tgataatacc actgcgatta ctgatgatga cttaccgttc tgattggaat
     6301 gattaaatgc cgaaaattac tagttatatc actcaagacg acggcacaac aacagttgtc
     6361 atctctgatg ttgaattagg caataaagaa acattactac ttgataacgg gtttgatgta
     6421 gaagtagatg taaacgttat agatccgttt caaattaccg gcaagcaacg tcgaaaaata
     6481 ttcgcgcttg tcaaagacat agaagaacat acaggtcaac caatggacta tatgagacat
     6541 atgttcatcg agtttgtaag aacgtactac ggctatgatg aacgtatttc gctaagtaat
     6601 tgtacgagaa cacaagcaag tcaaatcatt gaagcaacgc ttgactggac gttctacaat
     6661 gacataccac ttagctacaa aacgagtaat ctactgaaac aagataaatc attcttatac
     6721 tggtcaactg ttaaccgtaa ctgtgtaata tgcggaaagc ctcacgctga cctagcgcat
     6781 tacgaagcag taggtagagg catgaacaga aacaagatga atcactacga caaacatgta
     6841 ttagcgttat gtcgcgaaca tcataacgag caacatgcga ttggtgttaa gtcatttgat
     6901 gataaatatc aattgcatga ctcgtggata aaagttgatg agaggctcaa taaaatgctg
     6961 aaaggagaga aaaaggaatg aatagactaa gaataataaa aatagcactc ctaatcgtca
     7021 tcttggcgga agagattaga agcgctaaaa aaattaaaaa atttacccct gaggattcta
     7081 aaggttttcc tgatataaca aaagattcaa taaaagaacc taaataaaaa tattatggtt
     7141 gataaaatcc cattgttctt ttgttaacca cccttgtttg ttattgacta tttctgtaac
     7201 aaacagctta tctccagaat cgagataagg tttcaacttt tctatcattt ctgaagttga
     7261 taaagaagaa cggaataaaa atgaagattt ccaataattg caatgaccat tagaaatttc
     7321 cttttttata acatttctca attcctcata tttttgtccg ggtgagttta aatcatatgt
     7381 taacatataa ggtttttcca tattttattc acccccaatc taacgcagta gcgataacaa
     7441 aattatacca gaaaggagat aacgaaatgg caacatttag agtttacaaa gaatcaggta
     7501 actttgtcac agtacacaaa gattttatac atgattctaa tataagttgg aaggctaaag
     7561 gtattctact ttatttgtta agtcgacctg ataactggca aatttacgaa acagaactag
     7621 agcaacattc aactgatgga cttagcggtt taaagagtgg aatcaaggaa ctggaagaaa
     7681 ttggatacat tcaacgtagt agaaaacgtg ataaaagtgg taggttaaat ggttatgagt
     7741 acttagtata tgagcaaccg caccacattc gattttccaa cgttggaaaa accgttaacg
     7801 gtaaaaccaa caatggaaaa accgttaatg gtaaatcgca tactactaat aataatagta
     7861 ctaataatga tttaactaat aataacaata ctaataatga aggaagtata ttgtcgggca
     7921 acccgacggt gtcttccatt ccctataaag aaattatcga atacttaaat aaaaaagcag
     7981 gaaagcattt taaacataat acagctaaaa caaaagattt tattaaagca agatggaatc
     8041 aagattttag gttggaggat tttaaaaagg tgattgatat caaaacagct gaatggttaa
     8101 acacggatag cgataaatac cttagaccag aaacactttt tggcagtaaa tttgaggggt
     8161 acctcaatca aaaaatacaa ccaactggca cgaatcaatt ggaacgcatg aagtacgacg
     8221 aaagttattg ggattagggg gatattatga aaccactatt cagcgaaaag ataaacgaaa
     8281 gcttgaaaaa atatcaacct actcatgtcg aaaaaggatt gaaatgtgag agatgtggaa
     8341 gtgaatacga cttatataag tttgctccta ctaaaaaaca cccgaatggt tacgagtata
     8401 aagacggttg caaatgtgaa atctatgagg aatataagcg aaacaagcaa cggaagataa
     8461 acaacatatt caatcaatca aacgttaatc cgtctttaag agatgcaaca gtcaaaaact
     8521 acaagccaca aaatgaaaaa caagtacacg ctaaacaaac agcaatagag tacgttcaag
     8581 gcttctctac aaaagagcca aaatcattaa tattgcaagg ttcatacgga actggtaaaa
     8641 gccacctagc atacgctatc gcaaaagcag ttaaagctaa agggcatacg gttgctttta
     8701 tgcacatacc aatgttgatg gatcgtatca aagcgacata caacaaaaat gcagtagaga
     8761 ctacagacga gctagtcaga ttgctaagtg atattgattt acttgtacta gatgatatgg
     8821 gtgtagaaaa cacagagcac actttaaata aacttttcag cattgttgat aacagagtag
     8881 gtaaaaacaa catctttaca actaacttta gtgataaaga actaaatcaa aatatgaact
     8941 ggcaacgtat caattcaaga atgaaacaca atgcaagaaa agtaagagta atcggagacg
     9001 atttcaggga gcgagacgca tggtaaccaa agaatttttg aaaattaaac ttgagtgttc
     9061 agatatgtac gctcagaaac tcatagacga ggcacagggc gatgaaaata agttatatga
     9121 cctatttatc caaaaacttg cagaacgtca tacacgcccc gctatcgtcg aatattaagg
     9181 agtgttaaaa atgccgaaag aaaaatatta cttataccga gaagatggca cagaagatat
     9241 taaggtcatc aagtataaag agaatgagaa tgaagtttat tcgctcacag gagcccattt
     9301 cagcgacgaa aagaaaatta tgactgatag tgacctaaaa cgatttaaag gcgctcacgg
     9361 acttctatat gagcaagagc taggtttaca agcaacgata tttgatattt agaggtggac
     9421 gatgagtaaa tacaacgcta agaaagttga gtacaaagga attgtatttg atagcaaagt
     9481 agagtgtgaa tattaccaat atttagaaag taatatgaat ggcactaact atgatcgtat
     9541 cgaaatacaa ccgaaattcg aactacaacc taaatttggg aaacaaagac cgattacgta
     9601 tatagctgat ttctctttgt ggaaggatgg caaactggtc gaagttttag atgttaaagg
     9661 taaggcgact gaagttgcca acatcaaagc gaagatattc agatatcagt atagagatgt
     9721 gaatttaacg tggatatgta aagcgcctaa atacacaggt caagaatgga tggtatatga
     9781 ggacttagtg aaagtcagac gtaaaagaaa aagagaaatg aagtgattta atgcaacaac
     9841 aagcatatat aaatgcaacg attgatataa gaatacctac cgaagttgaa tatcagcatt
     9901 ttgatgatgt ggatgatgaa aaagatgcac tggcagatta cttatataac aatccggacg
     9961 aaatactaga gtatgacaat ttaaaaatta gaaatgtaaa tgtagaggtg gaataaatgg
    10021 cgaaaacagc aagaattgta aggatacatg ataaacctta taggttcagt aaatttgaaa
    10081 tggaattaat agaaagtcac ggtataacac ctggaatggt ttctaaaaga gtaaaagacg
    10141 gttgggaact acatgaagca atggacgcac cagaaggtac gcgtttaagt gagtacagag
    10201 aaaagaaaac aatagaaaga ctggaacaag ctagactcga acgcaaattg gaaagagaga
    10261 gaaagagaga ggctgagcta agaagaaaga agccacactt gtttaatgta ccacagaaac
    10321 attcacgtga tccgtactgg ttcgatgtca cttataacca aatgttcaag aaatggagtg
    10381 aagcttaatg agcataatca gtaacagaaa agtagatatg aacgaaatac aagacaatgt
    10441 taagcaacca gcgcactaca catacggcga cattgaaatt atagatttta ttgaacaagt
    10501 tacggcacag tacccaccac aattagcatt cgcaataggt aatgcaatca aatacttgtc
    10561 aagagcgccg ttaaaaaacg gacacgagga tttagcaaag gcgaagtttt acgtcgatag
    10621 agtatttgac ttgtgggagt gatgaccatg acagatagcg gacgtaaaga atacttaaaa
    10681 cattttttcg gctctaagag atatctgtat caggataacg aacgagtggc acatatccat
    10741 gtagtaaatg gcacttatta ctttcacggt catatcgtgc caggttggca aggtgtgaaa
    10801 aagacatttg atacagcgga agagcttgaa acatatataa agcaaagtga tttggaatat
    10861 gaggaacaga agcaactaac tttattttaa aagggcggaa acaatgaaaa tcaaaattga
    10921 aaaagaaatg aatttacctg aacttatcca atgggcttgg gataacccca agttatcagg
    10981 taataaaaga ttctattcaa atgatgttga gcgcaactgt tttgtgactt ttcatgttga
    11041 tagcatctta tgtaatgtga ctggatatgt atcaattaac gataaattta ctgttcaaga
    11101 ggagatataa caatgaaaat caaagttaaa aaagaaatga gattagatga attaattaaa
    11161 tgggcgcgag aaaatccgga tctatcacaa ggaaaaatat ttttttcaac aggatttagt
    11221 gatggattcg ttcgttttca tccaaataca aataagtgtt cgacgtcaag ttttattcca
    11281 attgatatcc ccttcatagt tgatattgaa aaagaagtaa cggaagagac taagtttgat
    11341 aggttgttag aggtatatga gattcaagaa ggagtctata aatccgcatt acacaaaggt
    11401 atcagtttga acgaacgttt tgaagacgac aatatttttc ctactaaagc attctacatc
    11461 ttaaacgatg acatgacgat gacattgatt tggaaagatg gggagttagt agaatgacat
    11521 tgagatttag agcatgggat aaattaggaa aggaaatgca caaagtaagt gctattgatt
    11581 ttagtagcaa aggcgcaaga attattcgtt tagctggagt acagtctaac ggtaaggaaa
    11641 atcataaaag atggcattca tctgttgaac tcatgcaata tacaggagtt aaagatgtga
    11701 atggtgtgga gatattcgaa ggcgatatag tgaaagttaa tgtgctagaa ggaattgggc
    11761 ctaatgcaag tattgttttt aaatacggta tgtttggcgt tgaaaatgac atgcatggat
    11821 acggttacga caaagggctc tatcctctaa acctcatcat taatagacac gaggtagaag
    11881 ttattggcaa tatatttgaa tcatctcatt tattggaggt aacagaatga actatgaaac
    11941 agggttccaa ctaggtgtaa tggaagctag gttgaagaag atgagaaaac aacgtgatga
    12001 gtacaagaag caacgcgatg aacttatcgt ggatatagcg aagttaagag aacgtaacaa
    12061 agagctagag aacatgtggc gcacgcttaa aaatgaattg cttggaagat acgaacatta
    12121 ctgttttaaa tatagagaac tacaccctga gagcaaagcg aacaggatag gagctatcta
    12181 tataggaggt aaaggcactg cagatattat actgtcgcga atggaagaac tagacggaac
    12241 aaatgagttt aacgaatttt tagatcgatt ggaggatgac actaatgaat aaccgcgaac
    12301 aaattgaaca atcagttatc agtgctagcg catataacgg taatgataca gagggattgc
    12361 taaaagagat tgaggacgtg tataagaaag cgcgagcatt tgatgaaata cttgagggaa
    12421 tgacaaatgc tattcaacat tcagttaaag aaggtattga acttgatgaa gcagtcggta
    12481 ttatggcagg tcaggttgtc tataaatatg aggaggcaca ggaaaatgaa gaaatttaat
    12541 gttcaaatca catataccgg catgattgaa gagactatcg aggctgaaag tttagaagaa
    12601 gcagaatttg aggcggatgt tactgcgaga ttggaagcac catttgattg tgatgagtat
    12661 gaaattaatg tagaggaggc acaggaaaat gactaacata ttaacaaccg atcagttaca
    12721 agagttaata caaatacaaa aggagttcga cgatagaata ccaactagaa atttaaatga
    12781 cacagtagct agtatgatta ttgaatttgt agagtggatt aacacacttg agttttttaa
    12841 aaattggaag aaacaaccag gtaagccact agatacacaa ttagatgaga ttgctgatta
    12901 cttagctttc agtttgcaat taactctgac tatagttgat gaagaagatt tggaagaaac
    12961 tactgaggtt atggttgatt tgattgaaaa tgaagttact ttgcctaaac tacattcagt
    13021 ttattttgtt catgtactgc atacgctaac agaacaattt gtaaaaggta ttgataatag
    13081 cattgtacaa gttttaataa tgccgttttt gtacgccaat acttactatt caatcgacca
    13141 actcattgaa gcatacaaaa agaaaatgaa aaggaaccac gaaagacaag atggaacagc
    13201 agacgcagga aaaggatacg tgtaaagata tattagatcg ggtcaaggag gttttgggga
    13261 agtgacacaa tatttaatca caacattcaa agattcaaca ggacgtaaac atacgcacat
    13321 aactaaagct aagagcaatc aaaggtttac agttgttgag gcagagagta aagaagaagc
    13381 taaagagaag tacgaggcac ggaatgcgcc agttgacgga gcaactaact taaacgatat
    13441 caaatcaaat attggtatct ttcacgttga aaaagttgaa ccaaacgagg gtatggtggc
    13501 tattaacatt gagacaatga aaccgttcga ggaggcagac catgattaac atacctaaaa
    13561 tgaaattccc gaaaaagtac actgaaataa tcaaaaaata taaaaataaa acacctgaag
    13621 aaaaatctaa gattgaaaat gattttatta aagatattaa tgataaagac agtgaatttt
    13681 acagtcctat gatggctaat atgaatgaac atgaattaag ggctatgtta agaatgatgc
    13741 ctagtttaat tgatactgga gatgacaatg atgattaaaa aacttaaaaa tatgggttgg
    13801 ttcgatatct ttattgctgg aatactgtca ttattcggca tattcgcatt gctacttgtt
    13861 gtcgcatttc ctatctatac agtgactagt taccaacaca aagaaacgca ccaaggaact
    13921 attacagata aatataacaa gagacaagat aaagaagaca agttctatat tgtattagat
    13981 aacaaacaag tcattgaaaa ctccgactta ttattcaaaa agaaatttga tagtgcagac
    14041 atacaagcta ggttaaaagt aggcgacaaa gtagaagtta atacgattgg atatagaata
    14101 cactttttaa atttatatcc ggtcttatac gaagtaaaga aggtagataa acaatgatta
    14161 agcaaatatc aagattatta ttcttactag cgatgtatga gctaggtaag tatgtaactg
    14221 agcaagtata tattatgatg acggctaatg atgatgtgga ggcgccgagt gacttcgcaa
    14281 agttgagcga tcattctgat ttgatgaggg cggaggtgtc ggagtagatg tatagcaaag
    14341 agtcaatcgt taatatgata ggcacacata aaatgaagtg taatgtgttg gctgatgtaa
    14401 taccggaata tgatagcaac tcaatagctc agtacggcat acaagcaaca ttaccaaagc
    14461 cacaagggga aaactcaagt aaagttgaag atgttgttgt gaggcttgaa agagcaaata
    14521 aaaggtatgc tcagatgtta aaagaagtcg agtttataaa tcaatcgcaa cagagattgg
    14581 gacatgttga cttttgtttc ttagagttgt taaagaaagg ctataacagg gatgcaatta
    14641 tcaagaagat gcctaactct aaattgaaca gaaataactt tctagcgcgc cgtgatgagt
    14701 tagcagaaaa gatttatcta ctacagtgac gaaaatgaca aaaatgacag aaatgacgaa
    14761 aatgacacta tttttaaact gtgaattaat tttatataat tgatttgtaa gaattatctt
    14821 aagacgtggg gtaatagcca cattagatgt tctcatcgat gtgattgaga agtgacaaac
    14881 atataaaaat tgatatgtta cgctattaat cacttactac ctgcctatat ggtgggtagt
    14941 ttaattcttg cattttgagt cataactatt ttcctccttt cacatttatt gaacgtagct
    15001 cctgcacgag atgtaggggc atttttatat ttaaaaaata acaagagtaa ttaacgtaaa
    15061 ggcgtgtgat acagtgaaaa caattgatta aattaacacc gaagcaagaa aagtttgtgc
    15121 taggactcat agagggcaag agccaacgga aagcatatat tgacgcaggg tattcgacta
    15181 aaggtaagag tggggaatat ctagataaag aagcgagtac actttttaaa aatcggaagg
    15241 tttccggaag gtacgaaaaa ttgcgtcaag aagtagctga acaatcaaaa tggacacgcc
    15301 aaaaggcctt tgaagaatat gagtggctaa agaatgtagc taagaatgac attgaaatag
    15361 agggagtgaa gaaagcgaca gctgatgcat tcctcgctag tttagatggt atgaatagaa
    15421 tgacgttagg taacgaagtt ttagctaaca agaaaataga aactgaaatt aagatgcttg
    15481 agaagaagat tgaacaaata gataaaggtg acagtggaac agaagataaa atcaaacaac
    15541 ttcacgacgc aataacggaa gtgatcgtca atgaataaac ttaaatcttt atatacggac
    15601 aaacaaattg aaatattgaa gcaaacgcaa aaacaagatt ggtttatgtt aattaatcac
    15661 ggagcaaagc gtacaggtaa aacaatatta aacaatgact tatttttacg tgagttaatg
    15721 cgtgtgcgaa agatagcaga cgaagaagga attgagacac ctcaatatat acttgctggt
    15781 gcaacattag gtacgattca aaaaaacgta ctaatagagt taactaacaa atatggcatt
    15841 gagtttaatt ttgataaata taattcattc atgttatttg gcgttcaagt ggttcagaca
    15901 ggtcacagta aagtaagtgg tataggagct atacgtggta tgacatcgtt tggtgcatat
    15961 atcaatgaag cgtcgttagc gcatgaagag gtgtttgacg agattaagtc acgttgtagt
    16021 ggaactggtg caagaatatt ggtagatacc aaccctgacc atcccgagca ttggttgttg
    16081 aaagattata ttgaaaatac agatcctaaa gcaggtatac tgagtcacca atttaagctc
    16141 gatgacaata actttcttaa tgatagatat aaagagtcta ttaaggcttc aacaccatca
    16201 ggtatgttct atgaacgtaa catcaacggt atgtgggtgt ctggtgacgg tgtagtatat
    16261 gccgactttg atttgaatga gaatacgatt aaagcagatg aactggacga catacctatc
    16321 aaagaatact ttgctggtgt cgactggggt tacgagcact atggatctat tgtgttaata
    16381 ggacgaggta tagatggtaa cttttatttt attgaggagc atgcacacca atttaagttt
    16441 attgatgatt gggtggttat tgcaaaagat attgtaagta gatatggcaa tattaatttt
    16501 tactgcgata ctgcacgacc tgaatacatc actgaattta gaagacatag attacgtgca
    16561 attaacgctg ataaaagtaa actatcgggt gtagaggaag ttgctaagtt gttcaaacaa
    16621 aacaagttac ttgttcttta tgataatatg gataggttta agcaagaggt atttaaatac
    16681 gtttggcacc ctacaaacgg agagcctata aaagaatttg atgacgtgtt ggactcgtta
    16741 agatatgcca tatacacaca tactaaacct gaacgattaa ggagggggaa atgacattgt
    16801 ataagttaat agatgatatt gaagcacaag gaatattgcc taagcatatt gaggctctaa
    16861 tagagtcaca taaagacgat agagagagaa tggttaatct ctataataga tacaagacac
    16921 atattgacta tgtaccaata ttcaaacgtc gaccaattga agaaaaagaa gattttgaaa
    16981 ctggtggaaa tgtaaggcga ttagacgtgt ctgttaataa caaacttaac aactcttttg
    17041 acagcgaaat tgttgataca cgtgttggtt atttacatgg tgttcctgtt acttatgatt
    17101 tagatgaaaa cgcagaaaaa aacgaaaagt tgaaaaagtt tataaccaac tttgccatta
    17161 gaaatagtgt tgatgatgag gattctgaaa taggtaaaat ggcagcaatt tgcggatatg
    17221 gtgctaggtt agcatatatt gatacgaatg gtgatattag gattaagaat atagatccct
    17281 ataatgttat ttttgttggc gacaatattt tagaacctac atactcattg cgctactttt
    17341 atgaaaaaga tgatgataat ggcactgatt atgtgtacgc agagttttac gataatgctt
    17401 attattatgt atttcgagga gaaggtattg acgctttgca agaagttgga cgatatgaac
    17461 atttatttga ttacaatcca ttgtttggtg tacctaacaa caaagagatg ataggagatg
    17521 ctgaaaaggt tattcactta attgacgcat atgatttaac aatgagcgat gcatcaagtg
    17581 agattagtca gacacgttta gcataccttg tgttacgcgg tatgggtatg agtgaagaaa
    17641 tgattcaaga aacacaaaag agtggcgcat ttgagttgtt cgacaaagat atggacgtta
    17701 aatacttaac aaaagatgta aatgacacaa tgattgagaa ccatttagat cgaatcgaaa
    17761 agaatatcat gcgttttgca aagtcagtaa actttaattc tgacgagttt aacggaaatg
    17821 tacctatcat tggaatgaaa cttaaactta tggctttaga gaacaagtgt atgacgtttg
    17881 agcgtaagat gacagctatg ttgaggtatc aattcaaagt tattttatct gcattaaagc
    17941 gtaaagggta caacttggat gatgatagtt atttaaacct gatatttaag ttcactcgta
    18001 acattccagt taataagtta gaagaatcac aagtgctaat taacctgaag ggacaagttt
    18061 cagaacgaac aaggttagga caatcacaac tagttgatga tgttgattac gaattagacg
    18121 aaatggaaaa agaaagtctt gaatttaatg acaaattacc tgacatagat gaaggtgacg
    18181 caaatgacaa atcccaaaat aaccaatcag aatgatatta atgagtatat cgagggttta
    18241 atctctaaag cagaaaaacc aatagaacaa ctatttgcta atcgacttaa agagataaaa
    18301 caaatcatcg cagatatgtt tgagaaatat caaaatgatg atgtgtatgt tacatggact
    18361 gaattcaata aatacaacag gctcaataag gagttaactc gtataggtac aatgttgact
    18421 gatgactata ggcaagtagc taagatgatt cagaagtcac aagaagatgc ttatatagaa
    18481 aaattcctta tgagccttta tttatatgaa atggcgagtc aaacatctat gcagtttgat
    18541 gttccgagta aagaggtaat caaatcagct attgaacaac ctattgagtt cattcgttta
    18601 atgccaacac tacaaaaaca tcgtgatgaa gtattgaaaa agatacgtat gcacattaca
    18661 caaggtatta tgagtggaga gggttactct aagatagcaa aagcaatacg cgatgatatc
    18721 ggcatgtcta aagctcaatc attgcgtgtg gctcgtacag aagctggcag agcaatgtca
    18781 caagctggac ttgatagcgc aatggttgct aaagataacg gtttgaagat gaagaaacgt
    18841 tggcatgcta ctaaagatac acgaacacgt gatactcatc gtcatttaga tggggaatca
    18901 gtggaaatag atcagaattt taaatcaagt gggtgtgttg ggcaggcgcc caagctattt
    18961 attggtgtaa acagtgcgaa agagaatatt aattgtcgtt gcaaattact ttattatatt
    19021 gatgaaaatg aattgccaac tgtaatgaga gcacgtaaag acgatggtaa aaatgaagtt
    19081 atcccattca tgacttatcg tgagtgggag aaatataagc gaaaaggtgg taattgatat
    19141 ggattttaaa ataaaagtaa atgttgatac tggcgaagct atagaaaagt tagaacgcat
    19201 taaatccttg tacgaagaga taatagagtt acaaaacgaa aaagttgttg taaacgtaac
    19261 agttaaaaat gaagctgatt tagatatggt taaaacatct attagcgaag aaaatgctaa
    19321 aaataatgat ttcacacttt tttagttgtc tctttgctac tcgaccttag catgtcgtta
    19381 aactgctttt tattatgcac ttttcggact gttagggtac gcgaagggca aaaaggagtt
    19441 ttgatatatg aatatcgaag aagttaagtc tttttttgaa gaacacaaag acgataaaga
    19501 agtaaaagat tatctaaacg gacttaagac ggtgtctgtt gatgacgtta aaggcttttt
    19561 agatacagaa gaaggtaaac gattcattca acctgaatta gatcgttatc attcgaaagg
    19621 attagaatca tggaaagaga aaaatcttga ggatctaatc gaacaagaag tacggaagcg
    19681 taatcctgag caatcagaag aacaaaaacg tattagtgct cttgaacaag agttagaaaa
    19741 acgcgacgca gaggcaaaac gtgagaagtt aagaagtaac gcgctaggta aagcgcagga
    19801 actaaattta ccaacatcct tagttgatag atttttaggc gattctgatg aagatactga
    19861 gcaaaactta aaagctttaa aagaaacctt tgacaagtat gttcaaaaag gtgttgagtc
    19921 taaatttaaa tcgagtggaa gagatgttaa agaatcacaa aatcaagatt tagacccttc
    19981 aaatgtaaag tccattgaag aaatggcgaa agaaatcaat attagaaaat aaagtgaggt
    20041 aataaaatat ggcaactcca acatacacgc caggcaatgt tattttatcg gattttaaaa
    20101 acggcgttat tccagcagaa caaggtactt taatcatgaa agacattatg gctaattcag
    20161 caattatgaa attagctaaa aatgagccaa tgacagcaca aaagaaaaaa tttacttact
    20221 tagcaaaagg tgtaggcgcc tactgggtat cagaaacgga acgtattcaa acttctaagc
    20281 ctgaatatgc acaagcagaa atggaagcta agaaaattgg tgtaattatt ccgttatcaa
    20341 aagagtttct taaatggact gcaaaagatt tctttaatga ggttaaacct ctaattgcag
    20401 aggcatttta caaagcgttt gaccaagctg ttatctttgg tactaaatca ccttacaaca
    20461 cttcaactag tggtaaaccg cttgttgaag gcgcagaaga gaaaggtaac gttgttacag
    20521 atactaataa tttatacgta gacctttcgg cattaatggc tactattgaa gatgaagagt
    20581 tagatccaaa cggagtatta actacacgtt cattcagaag taaaatgcgt aatgctttag
    20641 atgctaatga cagaccatta tttgatgcta acgggaacga gattatggga ttaccactat
    20701 cttatactgg agcggatgta tacgacaaaa agaaatcgtt agcactaatg ggtgattggg
    20761 attacgcacg ttacggtatc ttacaaggta ttgagtatgc aatttctgaa gatgccacgt
    20821 taacgacgtt acaagcatca gatgcttctg gccaaccagt atcattattt gaacgtgata
    20881 tgttcgcttt acgtgcgacg atgcatattg catacatgaa cgttaaacca gaagcgttcg
    20941 caacgcttaa accaactgaa taggaggaga tatgatggct aatcctgcag aagagattaa
    21001 ggtaaaaaaa gacaatatga ctattactgt tacaaagaag gcatttgact cttattacag
    21061 tcttgtcggt tacaaagagg ttaaatcacg tcgtactacg tctgataaga gcgagtgata
    21121 aaaatgactc tttatgaaga tgttaaactt ttactcaaga aaaatggagt ggaagttaaa
    21181 agtgatgaag aagaaatatt taagatggaa gttgacggaa tactagaaga tgttagggat
    21241 ataacaaaca atgattttat gaaagatggt caagtcattt atccttactc aatcaaaaag
    21301 tatgtcgcag atgtcctaga gtattatcaa cgacctgaag ttaaaaagaa tttaaagtca
    21361 agaagtatgg ggacagtgtc gtacacttat aacgatggtg tccctgatta cattagtgga
    21421 gtattaaaca ggtataaacg agcaaagttt catccgttta aaccaataag gtagaggtgt
    21481 tgtttgtgtt taacccatac gacgaattcc ctcacactat ttctattgga agtatcaaaa
    21541 aagtaggaga gtatccaatt atacaagagc gctttgtaag cgataaaaca attaaaggat
    21601 ttatggatac gcctactaca tctgaacaac taaaatttca tcaaatgtca caagaatatg
    21661 acagaaacct atatgtacct tatgacttgc caatatctaa aaacaattta tttgagtatg
    21721 agggtagaat ctttagtatt gaaggtgatt ctgtagatca gggcggacaa catgaaatta
    21781 agttactacg acttaagcag gtgccatatg gcaaaagtta agtacggtgc tgatagcatg
    21841 gttgttgaat tggataagtt cgataagaaa atagaagagt gggttaaaaa aggtatcgct
    21901 aaaacaacga tgaagatata taacactgct gtagcattag ctcctgctga cttaggattt
    21961 ttgaaagaaa gtatagactt tcgatttgag aacaacggtc taacaggagt tatcaatgta
    22021 ggtgcagaat atagtgttta tgttgagtac ggcacaggta tttatgccac taaaggtagt
    22081 cgcgctaaaa agataccgtg gagttataaa gacgctaacg gtaaatggca tactactaaa
    22141 ggacaagcgc cacagccgtt ttggaaccct gcaattgacg caggacgcaa gacgttcgag
    22201 cagtattttt catagaggtg gttaaatatg tgggtatcag ttgaacctga acttacaaat
    22261 caaatatata aaagattaat ctcagaccct aacattaaca aaatagttgg tgatagggtc
    22321 tttgacgttg ttcaagatga cgctgtttac ccatatattg ttgtgggtga atcaaacgtc
    22381 actaacaacg aatctagtgc aacaatgaga gaaacagttg gtattgtcat acatgtgtat
    22441 tcacagttcg ctacacaata cgaggctaag ctcattttaa gcgcaatagg ctatgtgcta
    22501 aacaggacta tagagataga aaattacgaa ttccaatata gctgtatcga tagtcaagca
    22561 gtattccctg atatagacag gtttactaag catggcacga tacggctttt atttaagtac
    22621 agacataaaa agaaaaacga aggagtgtat taaatggcgc aaaaaaacta tttagcagtt
    22681 gtacgtccag ctgaaactga cttagatcca gtagaatctt tattattagc tgacttacaa
    22741 gaaggtggac atacgattga aaatgattta gctgaaatag tacgaggcgg taaaacggac
    22801 tattctccca atgcaatgtc agaatcattt aaattaacaa ttggtaatgt gcctggagat
    22861 aaaggaattg aagcagtgaa acacgctgta caaacaggtg gacagttgcg tatatggctt
    22921 tatgagcgta ataaacgtgc agacggtaaa catcacggaa tgtttggtta tgttgttcca
    22981 gaatcatttg aaatgtcgtt tgatgatgaa agtgacaaaa tcgaactatc attaaaagtt
    23041 aaatggaata cagcagaagg tgctgaagat aacttgccga aagagtggtt tgaagctgca
    23101 ggtgcgccta cagttgaata cgaaaaattc ggcgaaaaag tcggaacatt cgagaatcaa
    23161 aagaaagcta gtgttgtatc tgattcacac acggaagacc attctatgta aactaataga
    23221 tcaagggggc gtaagctccc tattttttta taaaaaaatt gaaaagaggt atatattttg
    23281 actgaattta atccaattac aacattaaaa attaatgacg gagaaaaaga ttacgaagta
    23341 gaagcaaaag taacatttgc atttgaccga aaagctgaaa aattctctga agatagcgaa
    23401 gatgggagaa aaggagcaat gccaggattc aatgttatct ttaacggttt gctagaatct
    23461 agaaacaaag cgattttaca attttgggaa tgtgctactg cttatttaaa aaacccacca
    23521 actcgagaac aattagaaaa agcaattgat gatttcatca ctgaaaacga ggatactttg
    23581 ccgttattac aaggggcttt ggacaaactt aacaatagtg gttttttcaa gagggagagt
    23641 cgctcgtact ggatgacatt gaacaaagca ccgaatatgg ccaaaagcga ggacaaagaa
    23701 atgacgaaag caggcataga aatgatgaaa gagaattaca aggaaatcat gggcgcagaa
    23761 ccttacacga ttactcaaaa ataaggcaac tgacagctag atatttagga tatatccctg
    23821 aacatgaatt gttagcacta acacctgctg aatggcgtga ttggcttatt ggtggtcagg
    23881 ataggtacct agatcaaaga caattattaa ttgaacaagc gcaagctaac ggcttagtac
    23941 aagcttctaa gaggctaact agtatgattc gtgacattga gaaacaacgt tacgaaataa
    24001 gagaacctgg tagctatgct cgtgtacaaa aagttagatt agaagaagaa aaaagaagac
    24061 gtgaactctt caaagaaggt acaagaaagt tccttgaatc gaaaggaggt tagcctttgg
    24121 atactcattt tatggcaaag attatggcca atattagaga tttccaaagc aacgtaagga
    24181 aagctcaacg attagcaaag acgtctgtac caaacgaaat tgaaacagat gtaaaagcag
    24241 atatttcaag attccaaaga gctttacaac gcgctaaagc tatggcgcaa aaatggcgtg
    24301 aacataacgt taaaatagat ggtaataatt caccgttaaa acgtgcaatt gctagtgcaa
    24361 aaacgatgtt ggccacgtta cacaacaaaa caataaaagt taatttcgat acgagaggta
    24421 tgacaaaaac ccaaatttta actaaggcac tgaatcagtc cttaactgat tatagtgaga
    24481 aaatggacgc gctagctact aaaattcgta catttggtac aatttttgca caacaagtta
    24541 aaggcttaat gattgctagt atacaagcat tgataccagt gattgccggg ttagtacctg
    24601 caataatggc agtacttaat gcggttggtg tattaggtgg tggcgtttta ggtttagttg
    24661 gcgcattctc tgtcgcaggt cttggagttg ttggctttgg tgcaatggct attagcgctc
    24721 ttaaaatggt tgaagatgga acattggcag taacaaaaga agttcaaaac tttagagatg
    24781 cgagcgatca gttaaaaact acatggcgtg atattgttaa agagaatcaa gcaagtatct
    24841 ttaatgcgat gtcagcaggt atcagaggtg ttacaagtgc gatgtctcaa ttaaaaccat
    24901 tcttatccga agtatctatg ctggttgaag caaacgcacg caagtttgag gattgggtta
    24961 aacattctga aacaactaag aaagcgtttg aagcattgaa tagcataggt ggcgcaatct
    25021 tcggagattt attgaacgct gcaggaagat ttggcgacgg attaattaac attttcactc
    25081 aattaatgcc gttgttcaaa tttgtgtctc aaggactaca gaacatgtct atagctttcc
    25141 aaaattgggc taatagtgtg gctggtcaga atgctattaa agcgtttatt gactacacta
    25201 ccactaactt acctaagatt ggtcagatat ttggcaatgt gttcgctggt attggtaatt
    25261 taatgattgc ttttgctcaa aacagttcta acatttttga ctggttagtt aaattaactt
    25321 ctcaatttag agcatggtca gaacaagtag gacaatcaca aggatttaaa gactttatca
    25381 gttacgttca agagaatggt cctactatta tgcagttaat cggtaatatc gtaaaagcgt
    25441 tagtggcatt tggtactgca atggctccta tagctagtaa attactagat ttcattacta
    25501 atttagctgg atttatcgcc aaactattcg aagcacaccc agcagtcgct caaattatcg
    25561 gtgttatcgg tattttaggt ggcgtatttt gggctttaat ggctccgatc gcagctgtta
    25621 gcagtgtgtt aagtaatgtg tttagtatga ctttattgaa tgttgtcaaa agaatactgg
    25681 atttaactag aataactggg gtggtaagta aagcgttcgg tttattgact ggtgctttca
    25741 caagtatttc ttggccaata ttagcagtag ttgcagtcat tggtgtattc attggtattc
    25801 ttgtttattt atggaaaaca aacgagaatt tcagaaaaac aataacagaa gcttggaacg
    25861 gtattaaaac agcagtttcc ggtgcgattc aaggtgtagt agattggtta actcaattgt
    25921 ggggcaaaat tcaatcaaca ttacagccaa tcatgcctat attacaagta ttaggacaag
    25981 tattcatgca agttttaggt gttttggtaa taggcattat tacaaatgtt atgaatatca
    26041 tacaaggttt gtggacgtta attacaattg cgttccaagc cataggaaca gtgatatccg
    26101 tagcagtcca aatcatagta ggtttattca ctgctttaat tcagttgctt actggcgact
    26161 tctcaggtgc ttgggagact attaaaacta cggttaccaa tgtacttgat acgatttggc
    26221 aatacatgca atcagtttgg gagtcaatta tcggcttttt aactggcgta atgaatcgaa
    26281 cgctttcaat gtttggtaca agttggtcac agatatggag tacaatcact aattttgtta
    26341 gcagtatttg gaacactgtt acaagttggt tcagtcgtgt tgcttggagt gtggctgaaa
    26401 aaatgggaca agcattaaac tttattatca caaaaggttc tgaatgggtt tctaacattt
    26461 ggaatacagt tacaagtttc gcgagtaaag tagctgatgg gtttaaaaga gttgtctcaa
    26521 atgtaggtga cggtatgagt gatgcacttg gtaagattaa aagtttcttc agtgatttct
    26581 taaatgccgg agcggaatta atcggcaaag tagctgaggg tgtagccaat gctgcgcaca
    26641 aagtagtcag cgcggtaggc gatgcgattt catcagcttg ggactctgta acttcattcg
    26701 taagtggaca cggtggaggt agtagcttag gtaaaggttt agcggtatcg caagcaaaag
    26761 taattgctac agactttggc agtgccttta ataaagagct atcctctact ttgacagata
    26821 gtatagtaga tcctgtaagt acttctatag acagacacat gactagcgat gttcaacata
    26881 gcttaaaaga aaataataga cctattgtga atgtaacgat tagaaatgag ggcgaccttg
    26941 atttaattaa atcacgcatt gatgacatga acgctataga cggaagtttc aacttattat
    27001 aagggaggtt tgttagttga tagcgcacga tatagaagta ataaggaatg gttcacagta
    27061 tcgcgtcagt gacaatcctt tcacttataa tcacttggaa gtagttgaat ataacgttac
    27121 aggcgcagga tatcatcgta actattctga tatagagggt attgatggta gatttcataa
    27181 ttacgctaaa gaagaactta aaaaagtaga gcttaagcta aggtataaag tacctaaaat
    27241 tgcttatgct tcacatttaa agtcagacgt ccaagcatta tttgctggac gtttttattt
    27301 aagggaatta gctacaccag acaattcaat taagtatgag catatattag atataccaaa
    27361 agacaaacaa gcatttgagc ttgattatgt tgatggacga caactttttg taggattagt
    27421 aagtgaagtt tcttttgaca caactcaaac atcaggggaa ttttctttgt cgtttgaaac
    27481 aaccgaacta ccatactttg aaagtgtcgg ttatagtact gatcttgaaa gtgataacga
    27541 ccctgaaaaa tggtcggtac ctgatagatt gcctacaaac gaaggtgata agaggcgtca
    27601 aatgacattt cacaacacta actcaggaga agtttattat aacggtgatg ttcctttaac
    27661 acagtttaat cagtttaatg ttgttgaaat agagttagct gaagatgtta aagctaatga
    27721 taaggatgga ttcactttct atacagataa aggaaatatc tcagttatta aggaagttga
    27781 tttaaaagcc ggagataaaa taatcttcga cggtaaacat acctatagag gttatttaaa
    27841 tatagattct tttaataaaa ctttagaaca accggtttta tatccaggct ggaatcgatt
    27901 caagtctaat aaagtaatga aacaaattac atttagacac aaattatatt ttagataagg
    27961 agtagcctat gccaatttta ttaaaaagtc tacagggtgt agggcacgct attaatgtta
    28021 gtacaaaaat aagtaaaaag ctaaatgaag atagttcttt ggatctaact attatcgaga
    28081 acgcgagtac gtttgacgca ataggtgcta taactaaaat gtggacgatc actcatgttg
    28141 aaggtgaaga tgatttcaac gaatatgtaa ttgtcatact tgataagtct actattggcg
    28201 aaaaaataag gctagatatc aaagctaggc aaaaagaact tgatgacctt aacaattcta
    28261 ggatttacca agagtataac gaaagtttta caggcgtaga gttttttaac actgtattta
    28321 aaggaactag ttataagtac gtattgcaca ctaaggttga cgcatcaaag ttcgagggat
    28381 taggtaaggg agacacaaga cttgagatat ttaaaaaagg acttgaacgt tatcatctcg
    28441 aatatgaata tgaagctaaa actaagacgt ttcacttgta cgatgaatta tctaaagtag
    28501 cagattatta catcaaatca ggtgtaaatg ctgataatgt caaaattcaa gaagatgctt
    28561 ctaaatgcta cacatatata agaggctatg gcgactttga cggtcaacaa acttatacag
    28621 aggctggatt acaattcgaa ttcacacacc cattagcaca actgattggg aaaagggaag
    28681 cgcctccgtt aatagatgga cgtataaaaa aagaagatat tttaaaaaaa tcaatggagc
    28741 tagtgataaa gaaaagtgtc actgcttcta tttctttgga cttcgtagca cagcctgagc
    28801 attttccaga ggctaaccct agaataggtg atgttgttag agtggtggat tctgccatag
    28861 gatataacga cttagtgaga atagtcgaaa tcactacaca tagagatgcg tacaataata
    28921 tcactaagca agatgtagta ttaggagact ttacaaggcg taatcgttat aacaaagcag
    28981 ttcatgatgc tgcaaattat gttaaaagcg taaaatctac aaaatccgac ccatctaaag
    29041 aactaaaagc attaaacgca aaagttaacg caagtttatc tataaataat gaattggtta
    29101 agcagaatga aaaaataaac gctaaagtcg ataagatgaa tactaaaaca gttacaactg
    29161 ctaatggtac gatcatgtac gactttacta gtcaatcaag tataagaaac atcaaatcaa
    29221 ttggaacgat tggcgactct gtagctagag ggtcgcacgc aaaaactaat ttcacagaaa
    29281 tgttaggcaa gaaattgaaa gccaaaacga ctaatcttgc aagaggtggc gcaacaatgg
    29341 caacagttcc aataggtaaa gaagcggtag aaaacagcat ttatagacaa gcagagcaaa
    29401 taagaggaga cctaatcata ttacaaggca ctgatgatga ctggttacac ggttattggg
    29461 caggcgtacc gataggcact gataaaacgg atacaaaaac gttttacggt gccttttgtt
    29521 ctgcaattga agttattaga aagaataatc cagattcaaa aatactagtg atgacagcta
    29581 caagacaatg ccctatgagt ggtacaacaa tacgccgtaa agacacggac aaaaacaaac
    29641 tagggttaac acttgaggac tatgtaaaca ctcaaatatt agcttgtagt gagttagatg
    29701 taccagtgtt tgacgcatat cacacagatt actttaagcc atacaatcca gcttttagga
    29761 aagcgagcat ggaggacggc ttacacccta acgaaaaagg tcacgaggtt attatgtacg
    29821 agttaatcaa ggattattac agtttttacg actaaaggag gcaaccaatg gcttacggat
    29881 taattacaag tttacattca atgacaggtc ggaaaatagt tgctcaacac gagtataact
    29941 atcgcttgtt agatgaaggt atgagcaaac ttgagaaaat gtttatatac catcaaaaag
    30001 aagaaatata cgcacactca gcgaaacaaa ttaaatactt gaatgacagt gttgaagatt
    30061 atttaacgta tttaaatggc cgttttagca atatgattct aggccataac ggcgacggta
    30121 tcaatgaagt aaaagacgcg cgtattgata atacaggtta tggtcataag acattgcaag
    30181 atcgtttgta tcatgattat tcaacactag atgctttcac taaaaaggtt gagaaagctg
    30241 tagatgaaca ctataaagaa tatcaagcga cagaataccg attcgaacca aaagagcaag
    30301 aaccggaatt tatcactgat ttatcgccat atacaaatgc agtaatgcaa tcattttggg
    30361 tagaccctag aacgaaaatt atttatatga cgcaagctcg tccaggtaat cattacatgt
    30421 tatctagatt gaagcccaac ggacaattta ttgatagatt gcttgttaaa aacggcggtc
    30481 acggtacaca caatgcgtat agatacattg atggagaatt atggatttat tcagctgtat
    30541 tggacagtaa caaaaacaac aagtttgtac gtttccaata tagaactgga gaaataactt
    30601 atggtaatga aatgcaagat gtcatgccga atatatttaa cgacagatat acgtcagcga
    30661 tttataatcc tatagaaaat ttaatgattt tcagacgtga atataaagct tctgaaagac
    30721 aagctaagaa ttcattgaat ttcattgaag taagaagtgc tgacgatatt gataaaggta
    30781 tagacaaagt attgtatcaa atggatatac ctatggaata cacttcagat acacaaccta
    30841 tgcaaggtat cacttatgat gcaggtatct tatattggta tacaggtgat tcgaatacag
    30901 ccaaccctaa ctacttacaa ggtttcgata taaaaacaaa agaattgtta tttaaacgac
    30961 gtatcgatat tggcggtgtg aataataact ttaaaggaga cttccaagaa gctgagggtc
    31021 tagatatgta ttacgatcta gaaacaggac gtaaagcact tttaataggg gtaactattg
    31081 gacctggtaa taacagacat cactcaattt attctatcgg ccaaagaggt gttaaccaat
    31141 tcttaaaaaa cattgcacct caagtatcga tgactgattc aggtggacgt gttaaaccgt
    31201 taccaataca gaacccagca tatctaagtg atattacgga agttggtcat tactatatct
    31261 atacgcaaga cacacaaaat gcattagatt tcccgttacc gaaagcgttt agagatgcag
    31321 ggtggttctt ggatgtactg cctggacact ataatggtgc tctaagacaa gtacttacca
    31381 gaaacagcac aggtagaaat atgcttaaat tcgaacgtgt cattgacatt ttcaataaga
    31441 aaaacaacgg agcatggaat ttctgtccgc aaaacgccgg ttattgggaa catatcccta
    31501 agagtattac aaaattatca gatttaaaaa tcgttggttt agatttctat atcactactg
    31561 aagaatcaaa acgatttact gattttccta aagactttaa aggtattgca ggttggatat
    31621 tagaagtaaa atcgaataca ccaggtaaca caacacaagt attaagacgt aataacttcc
    31681 cgtctgcaca tcaattttta gttagaaact ttggtactgg tggcgttggt aaatggagtt
    31741 tattcgaagg aaaggtggtt gaataatgat agtagataat ttttcgaaag acgataactt
    31801 aatcgagtta caaacaacat cacaatataa tccaattatt gacacaaaca tcagtttcta
    31861 tgaatcagat agaggaactg gtgttttaaa ttttgcagta actaagaata ataagccgtt
    31921 atcaatcagc aaacataatg cgatgactag tattgtgctt aagacggata acttcgacga
    31981 tgaacacggc gcttatatta gtgatgaact tacaattgtt gatgcaatta acggacgaat
    32041 gcaatacgtt atcccaaatg agttcttaaa atacactgga cgcgtacatg cgcaagcata
    32101 ttttactcaa aacggtagca ataacgtaat tgtagagcgt caatttaact tcaatatcca
    32161 gaatgatcta attagtaatt ttgacggtaa aacaaagcta gtttatatca aatcaattca
    32221 ggacttaaca gaaagtgtta aagaagaagt tgaggactta aagaaaagtt tgagtgatac
    32281 aaaatcgttg gttactgaaa ttgatagtcg tattaatcaa ggtattcaaa gattagaaat
    32341 caaacaaaat gaagcggtac agatgattac aacaacacaa gacaaagccg ttcaatatat
    32401 aaatagcgag ttccagaaaa ttgttgataa agagcaagcg atttttgaac gtgttaacga
    32461 agttgaacaa caaatcaatg gcgctgacct tgttaaaggt aattcaacaa caaattggca
    32521 aaagtctaaa cttactgatg attacggtaa agcaatcgaa tcatctgaac agtcaataga
    32581 agctgtttta agacacgcta actcatctat gattattcat attactaatg caaaagatgc
    32641 gccagaaaag gcggatatag gcacgttaga gaagcccgga caagatggtg ttgatgacgg
    32701 ttcttcgttc gatgaatcaa cttatacatc aagcaaatct ggtgtgttag ttgtttatgt
    32761 tgttgataat aatactgctc gtgcaacatg gtacccagat gattcaaacg atgagtacac
    32821 aaaatacaaa atctacggca cgtggtaccc gttttataaa aagaatgatg gaaacttaac
    32881 taagcaattt gttgaagaaa tatctaacaa cacactgaat caagctaaac agtatgtaga
    32941 tggtaagtta caaagtataa gttggcaaca acataagtta acagaacata acggtcaatc
    33001 aatccaaaag aacttatata acgccaaagg taatttagaa gcattgggcg ctgggaatta
    33061 ttacgtaaca agtgtgcctg atttaccagg tattgttgaa agttacgaag gctacttatc
    33121 agtatttgtt aaagatgatg caaataagtt attcaacttc acacctttaa actctaaaaa
    33181 agtttataca cgatcaatca ccaatggtcg attagattca caatgggcta cacctaacga
    33241 acataaaaca gccgtgttat tcgacggtgc tgcaaacggt gtaggaacaa ggattaattt
    33301 aaccgaagct tatacaaatt atgcaattct attcataagc ggtacttatc caggtggtgt
    33361 tattgaagca ttcagtttaa cctctatacc aaatgcgatt caattaagta aaacaaatgt
    33421 agttgactca gacggtaacg gtggtggtag ttatgaatgc ttaataacta aagaaagtgg
    33481 tacgacgtta aaaatcgata acgatgtgta ccttgattta ggcagtaaaa caggttctgg
    33541 tgctaatgca aacagagtta cgataaataa aattgtgggg tggaaataat gaaaatcaca
    33601 gtaaacgata aaaacgaagt tatcggatac gttaatactg gcggtttacg caatagttta
    33661 gatgtagacg ataacaatgt gcctatcaaa ttcaaagaag agttcgaacc tagaaagttc
    33721 gttttcacta acggcgaaat taaatacaat agcaatttcg aaaaagaaga cgtaccgaat
    33781 gcatcaaacc aacaaagtga atcagatttg agtgatgaag aacttcgcgg aatggttgca
    33841 agtatgcaaa tgcaggtgac gcaagtaaac attttggcga tggaattaaa gcaacaaaac
    33901 gctatgttaa cacaacagtt gactgaacta aaagctggta aaacaaatac agaggaggac
    33961 gtttaaatgg agaaaattaa gatgatttat ccaactttca aggacattaa aactttttat
    34021 gtgtggggtt gctataaaaa tgagcaaatt aagtggtacg tagacatggg tgtaatcgac
    34081 aaagaagaat atgcattgat cactggtgaa aaatatccag aggcaaaaga tgaaaagtca
    34141 caggtgtaat gcttgaggct ttttaattta acacaaagta ggtggcgtaa tgtttggatt
    34201 taccaaacgg cacgaacatg aatggcgaat tagaagatta gaagagaatg ataaaacaat
    34261 gcttagcact ctcaatgaga ttaaattagg tcaaaaaact caagagcaag ttaacattaa
    34321 attagataaa actttagatg ctatccagag ggaaagacag atagacgaaa aaaataagaa
    34381 agaaaacgac aaaaatatac gcgatatgaa aatgtggatt ctcggtttga tagggactat
    34441 cttcagtacg attgtcatag ctttactaag aactattttt ggtatttaaa ggaggtgatt
    34501 accatgctta aagggatttt aggatatagc ttctgggcgt gcttctggtt tggtaaatgt
    34561 aaataacagt taagagtcag tgcttcggca ctggcttttt attttgattg aaatgaggtg
    34621 catacatggg attacctaac ccaaagacta gaaagcctac agctagtgaa gtggtggagt
    34681 gggcaaagtc gaatattggt aagaggatta atatagataa ttatcggggc agtcaatgtt
    34741 gggatacacc taactttatt tttaaaagat attggggttt tgtaacatgg ggcaatgcta
    34801 aggatatggc taattacaga tatcctaagg gtttccgatt ctatcgttat tcatctggat
    34861 ttgtaccgga acctggagac atcgcagttt ggcaccctgg caacggaata ggttcggacg
    34921 gacacaccgc aatagtagta ggaccatcta ataaaagtta tttttatagc gttgaccaaa
    34981 actgggttaa ttctaatagt tggacaggtt ctccgggaag tttagtaaga cacccttatg
    35041 taagtgttac aggctttgtc agacctccat attcaaaaga tactagcaaa cctagtagta
    35101 ctgatacaag ttcagcatca aaagccaatg actcaacaat tactggcgaa gcgaagaaac
    35161 cgcaatttaa agaagttaaa acagtaaaat acactgctta cagcaatgtt ttagataaag
    35221 aagagcattt cattgatcat atagttgtaa tgggtgatga acgctcagat attcaaggat
    35281 tatatataaa agaatcaatg catatgcgtt ctgtagacga actgtatacg caaagaaata
    35341 agtttataag cgattatgaa ataccgcatt tatatgtcga tagagaggct acatggcttg
    35401 ctagaccaac caattttgat gacccgcgtc accctaattg gctagttatt gaagtatgtg
    35461 gtggtcaaac agatagcaag cgtcaattct taatgaacca aatacaagct ttaatacggg
    35521 gtgtatggtt gttgtcagga acagataaag aattatctga aacgacgtta aaggtagacc
    35581 ctaatatttg gcgtagtatg aaagatttaa ttaattacga cttgattaag caaggtatac
    35641 cggatgacgc aaagtatgag caagtcaaaa agaaaatgct tgagacgtac atcaaacgag
    35701 atatattgaa acgagaaaat attaaagaag taactacaca aacaacaata agaattagtg
    35761 ataaaacatc ggttgacagt gcgtcaacaa gaggacccac tgcatcagac gaaaaaccaa
    35821 gcatcgttac tgaaaaaagt ccgttcacgt tccagcaagc actggataga caaatgtcta
    35881 ggggtaaccc gaaaaaatct catacatggg gctgggctaa tgcaacacga gcacaaacga
    35941 gctcagcaat gaatgttaag cgaatatggg aaagtaacac acaatgctat caaatgctta
    36001 atttaggcaa gtatcaaggc gtttcagtta gtgcgcttaa taagatactc aaagggaaag
    36061 gaacgctaga cggacaaggc aaagcatttg cagaagcctg taagaaaaac aacattaacg
    36121 aaatctattt gatcgcgcac gctttcttag aaagtggata cggaacaagt aacttcgcta
    36181 gtggtagata cggtgcatat aattacttcg gtattggtgc attcgacaac gaccctgatt
    36241 atgcaatgaa atttgctaag aataaaggtt ggacaactcc agcaaaagca atcatgggcg
    36301 gtgctagctt cgtaagaaag gattacatca acaaagggca gaatacactg tacagaatca
    36361 gatggaatcc taagaatcca gctacgcacc aatacgctac tgctatagag tggtgccaac
    36421 atcaagctag tacaatcgct aagctatata aacaaatcgg cttaaaaggt atctacttta
    36481 taagagataa atataaataa agaggtgtat aaatgtacaa aataaaagat gttgaaacga
    36541 gaataaaaaa tgatggtgtt gacttaggtg acattggctg tcgattttac actgaagatg
    36601 aaaatacagc atctataaga ataggtatca atgacaaaca aggtcgtatc gatctaaaag
    36661 cacatggctt aacacctaga ttgcatttgt ttatggaaga tggctctata ttcaaaaatg
    36721 agccccttat tatcgacgat gttgtaaaag ggttccttac ctacaagata tctaaaaagg
    36781 ttatcaaaca cgctggttat gtacgttgta agctgttttt agagaaagaa gaaggaaaaa
    36841 tacatgttgc aaacttttct ttcaatatcg ttgatagtgg tattgaatct gctgtagcaa
    36901 aagaaatcga tgttaaattg gtagatgatg ctattacgag aattttaaaa gataacgcga
    36961 cagatttatt gagcaaagac tttaaagaga aaatagataa agatgtcatt tcttacatcg
    37021 aaaagaatga aagtagattt ataggtgcga aaggtgataa aggcgaaccg ggacaacctg
    37081 gtgcaaaagg tgaagcaggt aaaaaaggag aacaaggcgc acccggtaaa aacggtactg
    37141 tagtatcaat caatcctgac actaaaatgt ggcaaattga tggtaaagat acagatatca
    37201 aagcagaacc tgagttattg gacaaaatca atatcgcaaa tgttgaaggg ttagaaaata
    37261 aattgcaaga agttgaaaaa atcaaagata caactctcaa cgactctaaa acgtatacgg
    37321 attcaaaaat tgctgaacta gttgatagcg cgcctgaatc tatgaataca ttaagagaat
    37381 tagcagaagc aatacaaaac aactctattt cagaaagtgt attgcaacag attggctcaa
    37441 aagttagtac agaagatttt gagagattca agcaatcatt aaacagtttg tatgcagata
    37501 aaaatcatag tcatacaatc aaacagattg aaggattaga aaatgcttta tcaaaaaaat
    37561 cagacataaa tcacagtcat gatgaacgtt atcttttatc atcaaatgct tttacaaaag
    37621 aggaagcaga taaactttat caacctatcg gttcttcgca gccgtcactg aatatttgga
    37681 caggcagtga aacagaatat aattatttgt atcaaaaaga ccctaataca ctttacttaa
    37741 ttaaggggtg attttatgga aggtaatttt aaaaatgtaa agaagcttat ttacgaaggc
    37801 gaagaatata caaaagtata tgctggaaat atccaagtat ggaaaaagcc ttcatctttt
    37861 gtaataaaac ccttacctaa aaataaatat ccggatagca tagaagaatc aacagcaaaa
    37921 tggacaataa atggagttga acctaataaa agttatcagg tgacaataga aaatgtacgt
    37981 agcggtataa tgaggatttc gcaaactaat ttagggtcaa gtgaattagg aatatcagga
    38041 gtcaatagcg gagttgcaag taaaaatatc aactttagta atccttcagg gacgttgtat
    38101 gtcactataa gtgatgttta ttcaggatct ccgacattga ccattgaata attttaaacg
    38161 actaattttt tagtcgtttt ttattttgga taaaaggagc aaacaaatgg atatcggtac
    38221 aatcgtaaga acaattttat taatagtcgc atggatcaat cagtttttag caatcaaaca
    38281 tatttctcca atcccagttg acgaagtgtt tataagcaca gtcgttactg ggattgtttc
    38341 aatttggacg tggtggaaga ataacaactt tactcacgca tctaagaaag ggcaacaaaa
    38401 aatttatgaa gtaaaagctg gcattcagtc aactggtggc gcacctaaag tgaacggaga
    38461 tgataacaat gccgtcggtt agaacataca gtcaagctat tagctacctt aaaagcctag
    38521 agggtaaggc gtggaatcca gacaatgcat ttggatgtca atgcttcgat actgccaacc
    38581 aatattggct ttacttattt aatcataggt tgaaaggtgt gggcgctgcg gacattccta
    38641 catggaatga tttcactaac gaagcaaccg tttacgaaaa tactgtgtcg tttcaagcat
    38701 tgccgggcga cgtcgttatt tttaaccgta attatggcgg tggttatggt catgtaggta
    38761 ttgtaataag cgctacgtta gattctataa ctattttaga gcagaactgg ctaggcggtg
    38821 cttactggag cccaccagaa gttactacaa gacgcacaca cggctatgat tttcctatgt
    38881 ggttcattcg tccattctat gcaaaagaaa cgactgctaa taagctaaga agcgcagtaa
    38941 caccagttaa acaagataag ttatcaaaag gcaaaaaaat catgcttgta gctggtcatg
    39001 gtattggcgc atactctaac gacccaggtg ccgttgcgaa tggagaaaac gaaagagatt
    39061 ttaaccgtaa aaatattatc cctagagtga aaaagtatct tgagtcagta ggtaacacag
    39121 tattgttata cggtggcaac tcgatgaatc aagatttata tcaagataca ttgtacggtc
    39181 aacgtgttgg aaactataaa gattatggca tgtactggat taaaagcgaa gtcaaaccgg
    39241 atgcaatcat agagtttcat ttagattctg ctagcccaca agcaagtggc gggcatgtaa
    39301 tcattagcga tcgtttccca gctgatgaca ttgacaaggc attaagtagt gcattagata
    39361 aaacagtggg taaaataaga ggtgtgacac ctagagggga tttattgaac gctaacgtgt
    39421 ctgctgatct taatcttaat tatcgtttaa tcgaattagg ttttatcaca tctacgaaag
    39481 atttaaacta cattaaaaac aatttagaca gcttcacgaa gcggattgct gaagccatta
    39541 acggcagaca aattgatgcg ccaagtagta agccaagcgc tgacaaaata acatggaatt
    39601 ggaaaggcgt attttatcct aatccagaaa aagctataag agtcagaaaa acagctggat
    39661 taaccggcac agtcgttgaa gaagattcat ggctatacac aaaagatgat tgggtaaaat
    39721 tcgaccaagt cattaaaaaa gatggctact ggtggattag attcaaatat caacgtgagg
    39781 gctctagtac taacaatttc tattgtgcag tgtgtagaat tactgataag gaacaaaaga
    39841 ttaaaaatga aaaatattgg ggcacgattg agtgggctta ataggttgta cctataaaaa
    39901 gaaaagaggt aggttatttt cttcctacct ctaaaaatga ttatctttct attgttatat
    39961 gagttatatc tttaggacta atcagtctat tttttacatt agaatcttga tctcctacct
    40021 tgccatatac tttttcatca gaaggatctt tactatggat agttacttta tcaccgactt
    40081 taacaatatg cttttctttt aatttatcta ctaatttttt ccatgcatca tttgcctcta
    40141 ttgtgtttcc gtttggatta actcttgtaa tatcgacacg gttaacgcta tcaggactaa
    40201 cggtgctgtt attagtatta ctaagattat ctaagttcgc agtcccagaa atttcgctct
    40261 ctccaccgtt ttttaattta tattttactt taatcgtttc gttgtttgtt ttatcaatga
    40321 tatttgcgtc ttttaaagcg tctcttacat ttttccacaa ttcgctatct gttatttcag
    40381 aagcttttgc aacgttatta ataccattat aatttgaaga agaatgaaaa cctgaaccta
    40441 ctgttgttaa aactaaagca cttgctatca atgtttttgt taatagtttt ttattcattt
    40501 tattttctcc tat
//
LOCUS       DAXWFK010000007        33392 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.007, whole genome shotgun sequence.
ACCESSION   DAXWFK010000007 DAXWFK010000000
VERSION     DAXWFK010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 33392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33392
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.007"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..135)
                     /locus_tag="ACGEXS_000262"
     CDS             complement(<1..135)
                     /locus_tag="ACGEXS_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFF8011311.1"
                     /translation="MKKYDIAVLDFETMNEHMNSPCEVAVSLIKDLSIVKVYSSYINP
                     P"
     gene            complement(151..864)
                     /locus_tag="ACGEXS_000263"
     CDS             complement(151..864)
                     /locus_tag="ACGEXS_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFF8011312.1"
                     /translation="MNKERNIIIAKNIRKFLNDSNMSQKKLAELINIKPSTLSDYLNL
                     RSNPSHGVIQRIADVFEVGKSDIDTTYKDDNDITSIYNKLTPPRQENVLNYANEQLEE
                     QNSKGDNVVDINSYKQEKTPVNVNGCVSAGVGERLHDETLFTEMVKGPIPTHDLALKV
                     NGDSMEPMFKDGEIIFVEKTHNIKNGQIGIFIIEEEAYVKKVFVEDDRLTLVSLNKDY
                     DDLHFYRNESVRLIGKVIL"
     gene            827..1000
                     /locus_tag="ACGEXS_000264"
     CDS             827..1000
                     /locus_tag="ACGEXS_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFF8011313.1"
                     /translation="MFLAIIIFLSLFILLTSFFLIIRNFHIIVKFFTKKTYLMLTIRK
                     FRIILGLRKAVTT"
     gene            997..1260
                     /locus_tag="ACGEXS_000265"
     CDS             997..1260
                     /locus_tag="ACGEXS_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8011314.1"
                     /translation="MKTLKELRTDYGLTQKELGDLFKVSSRTIQNMEKDSTNIKDSLL
                     SKYMSAFNVKYDDIFLGNEYENFVFTNDKKKSIILAFKEKQTS"
     gene            1276..1491
                     /locus_tag="ACGEXS_000266"
     CDS             1276..1491
                     /locus_tag="ACGEXS_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MW1434 family type I TA system toxin"
                     /protein_id="HFF8011315.1"
                     /translation="MNIQEATKLAMEKGISIRRENQDVYGILPTNLQRYQCLVVSRHY
                     KKKRQTAAGRWQPSADDLIADDWILDY"
     gene            complement(1480..1809)
                     /locus_tag="ACGEXS_000267"
     CDS             complement(1480..1809)
                     /locus_tag="ACGEXS_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011316.1"
                     /translation="MTDEAKFVLLQLYSIYLDRIDEGMSKRSASYFGSDESSFNAFFL
                     GFNFEDYIDAVLELKHRDFVIASAEDGGFLEMALSREGIAYSESESKKDYKTLMGLIR
                     DLKKLII"
     gene            1860..2612
                     /locus_tag="ACGEXS_000268"
     CDS             1860..2612
                     /locus_tag="ACGEXS_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFF8011317.1"
                     /translation="MQALQTKSNIGEMFNIQEKENGEIAISGRELHQALEVKTPYKKW
                     FERMSDYGFEENIDYIVTDIFVHNPLGGRQNQTDHALTLDTAKEIAMIQRSEPGKRAR
                     QYFIQVEKAWNSPEMIMQRALKIANNTINQLETKIERDKPKIVFADAVATTKTSILVG
                     ELAKIIKQNGINIGQRRLFEWLRQNGFLIKRKGVDYNMPTQYSMERELFEIKETSITH
                     SDGHTSISKTPKVTGKGQQYFVNKFLGEKQTS"
     gene            2628..2825
                     /locus_tag="ACGEXS_000269"
     CDS             2628..2825
                     /locus_tag="ACGEXS_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011318.1"
                     /translation="MQAQNKKVIYYYYDEEGNRRPVNIQYNDGYDLMIDQRFIEMTLE
                     RHPHLKNNFYGLIDGKEFKLD"
     gene            complement(2812..3192)
                     /locus_tag="ACGEXS_000270"
     CDS             complement(2812..3192)
                     /locus_tag="ACGEXS_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2513 domain-containing protein"
                     /protein_id="HFF8011319.1"
                     /translation="MKLDHDCVRHLLLEIETNKKIGEPLTEYNFKDNVVFGKYDFETV
                     MYALLKLEEAKYVSVKFGWEDGHIYGYTINDITWSGHEFLDNIRDNHTWKEVKKVANK
                     TTSMSVTLLSKLAFNYLTQKFNLT"
     gene            3247..3570
                     /locus_tag="ACGEXS_000271"
     CDS             3247..3570
                     /locus_tag="ACGEXS_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001806402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFF8011320.1"
                     /translation="MPHILNVTVPIPETHVLITKDEYEELIAYSLDPVWNMSDLKKKL
                     KIASDETIKDRLLFHPRLEKELRAQGIVHYPDENFNRWRFNARRMHKFVDEHFNEIYK
                     GGHNK"
     gene            3567..3728
                     /locus_tag="ACGEXS_000272"
     CDS             3567..3728
                     /locus_tag="ACGEXS_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 family protein"
                     /protein_id="HFF8011321.1"
                     /translation="MSKTYKSYLVAVLCFTVLAIVLMPFLYFTTAWSIAGFASIATFI
                     FYKEYFYEE"
     gene            3823..4125
                     /locus_tag="ACGEXS_000273"
     CDS             3823..4125
                     /locus_tag="ACGEXS_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2482 family protein"
                     /protein_id="HFF8011322.1"
                     /translation="MTKNYKDMTQEEIKDLLSEKTAELYELAKEIKGESKFDILLFSS
                     IGVIDGDYLAGSSSVIGHTFDLAYLLDSTKSYKDIVNVLQMCKSQKILGIDDDKED"
     gene            4130..4390
                     /locus_tag="ACGEXS_000274"
     CDS             4130..4390
                     /locus_tag="ACGEXS_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1108 family protein"
                     /protein_id="HFF8011323.1"
                     /translation="MYYKTGDVCRKIFNVDGFDFQLRVKKRAYSVEIVVLDHEGNSID
                     GLLVSDENDLYTALDILKQSIYEWIENNTDEQDRLINLVMKW"
     gene            4399..4662
                     /locus_tag="ACGEXS_000275"
     CDS             4399..4662
                     /locus_tag="ACGEXS_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001638663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011324.1"
                     /translation="MRDTERNILNIFKTLFDEYTLSNQRALLEIERNHHGYLSINFLH
                     YHDSYKTNNKLVQIHEINPDSHERIKNLIIEVLRGHRKIKKGA"
     gene            4671..6614
                     /locus_tag="ACGEXS_000276"
     CDS             4671..6614
                     /locus_tag="ACGEXS_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8011325.1"
                     /translation="MKINKLTISNFAGIKEEKFNFDGKDAKIYGNNATGKTTTATALQ
                     WLLFDKGLDGSTKSFNPVPLNEKNEENYELIPTVFAEFEIDGKITTFKKESHPKYTIN
                     QKTNRKEYSRSRTKKQYINDESIKVKDYKARIDELIDEDVFKLITNPQAFNLLDWKKR
                     RSLLFEIAKPINDEDVIKTNDDFKELNNILGDHEIETKKKILTDKIKQINKDIKDIPI
                     RINQTQQNKQDVPEFDNDRHTIIKQEIEQLENERIDIQNGAEEINLRNQLADKQSELK
                     RIEANNSASNENKIHALTNELHVENGTVANLKTRLKQNKQQITHEENRRNQLLENHKG
                     LKSDLEKAKNQKFEYLDDNVCSCCGQQLPAEQVSEVREKALQKFNANKSKELETIQTS
                     INHIISEGKKIKPIIEKLEDDNNNLQIKINEAEERSARIQNKINKLKTTHVDVTQTDE
                     YKAVMLEINEINQKRSNIRKTIQDKVSGIDDKISELTQEKSEIEVSISIEKSNKHLDD
                     VISELRNEEDRLLDEKEKYSHDLYILKEFTTTKVKMLTENINNEFDIAEFKLFNTLVN
                     GELEETCSTTVNGVEYDSGLNNASRINVGLDIINTLSKHFKVTAPIFIDNAESVTELI
                     KTESQQIQLIVNEQDKKLRMETI"
     gene            6616..7536
                     /locus_tag="ACGEXS_000277"
     CDS             6616..7536
                     /locus_tag="ACGEXS_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFF8011326.1"
                     /translation="MTENNKLQTIEQQLVQEKNVSDNVLNKVRVLESQGNLELPNDYS
                     PSNAMKQAWLQISQDNKLMSCNDTSKANALLDMVTQGLNPAKNQCYFIPYGNKMQLQR
                     SYHGNVMMLKRDAGAQDVVAQVIYKGDTFKQEMGETGRIKAIKHEQDFFNIDKENIIG
                     AYCTIVFNDGRDNYIEVMTIEQIKQAWMQSSMIKDEKALQNSKTHNNFKEEMAKKTVI
                     NRAAKRYINTSTDSNLFKYAQESEQRQRKEVLDAEVEENANQEQLDFEQPVLEEAQYT
                     ELENDKPIDVSDFEEIKEPATEKESEEEPF"
     gene            7617..8234
                     /locus_tag="ACGEXS_000278"
     CDS             7617..8234
                     /locus_tag="ACGEXS_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFF8011327.1"
                     /translation="MKFERVQKHFKYKTRHIAGCLITHEHGDHAKYTKQFVDNGVISY
                     MTAGTQQAMNFESHRLCTIKAKQELRIGTWSILPFDIEHDANEPVAFLLQSTLGYKVL
                     YVTDTKYLKYKFNGITHMMLEVNYIYEQMQENIKNGSVHSTLANRIMESHFSLEHAIG
                     MLKANDLTRLEEIHLIHLSSQNSNAKYIKSEIQKVTGAPVYFGGL"
     gene            8235..8705
                     /gene="ssb"
                     /locus_tag="ACGEXS_000279"
     CDS             8235..8705
                     /gene="ssb"
                     /locus_tag="ACGEXS_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFF8011328.1"
                     /translation="MLNRTILVGRLTRDPELRTTQSGVNVASFTLAVNRTFTNAQGER
                     EADFINIIVFKKQAENVNKYLSKGSLAGVDGRLQTRNYENKEGQRVYVTEVIADSIQF
                     LEPKNSNDTQQDLYQQQVQQTRGQSQYSNNKPVKDNPFANANGPIELNDDDLPF"
     gene            8735..9628
                     /locus_tag="ACGEXS_000280"
     CDS             8735..9628
                     /locus_tag="ACGEXS_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFF8011329.1"
                     /translation="MTGWISIDRSIQNHWLFKEKRTFSKFEAWIYLLMEANHSEAKVP
                     IGNQIVTVERGQRLTSILTLSDLFNWSRFKVKTFLDLLESDGMLEVKTTSKYTLITIV
                     NYDFYQSEQGRNQHQNDIKPTSKQHQSNINPTSKQHQTNTNNNDNKDNNEKNVNNEKK
                     KVTAFDFFQDNGFGFITPYNLDDLNYYLDSFENDSDEIVTASLKIAKDRNKVTWGYAK
                     SILNTWLNANLKSIEQVRAFEKQQLESKKQNYKPFVKQSKEKTPKWLTDSTRETKTPE
                     VDENLEKDREAFIKRLNSKWE"
     gene            9635..9853
                     /locus_tag="ACGEXS_000281"
     CDS             9635..9853
                     /locus_tag="ACGEXS_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011330.1"
                     /translation="MDAFDKYYLFDHDGNKMFSVTPHFKDGRHLVVGLKHTKFNGRRW
                     YLDDYELKTLIDNEQMELGHQTSLFEYI"
     gene            9862..10266
                     /locus_tag="ACGEXS_000282"
     CDS             9862..10266
                     /locus_tag="ACGEXS_000282"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0000400 - four-way junction DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFF8011331.1"
                     /translation="MEIEIKFNETFEAPMGSPRPRFSTKGRYAHTYMPTKYTEHKKYL
                     QNQMPKLNLENALKIELEFYFTPPKSWSKKKKTQAIGQLKVTKPDIDNLMKTVLDACN
                     NYLWKDDNQIAEITSSKRYGIEPKIIIRIEEI"
     gene            10279..10647
                     /locus_tag="ACGEXS_000283"
     CDS             10279..10647
                     /locus_tag="ACGEXS_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SA1788 family PVL leukocidin-associated protein"
                     /protein_id="HFF8011332.1"
                     /translation="MARKARIVTINDKPYRFTKSEMELIESHGITAGMVSKRVKDGWE
                     LHEAMDAPEGTRLSEYREKKTIERLEQARLERKLERKRKKEAELRRKKPHLFNVPQKH
                     PRGRYACYLMENDIFVKVKK"
     gene            10651..10893
                     /locus_tag="ACGEXS_000284"
     CDS             10651..10893
                     /locus_tag="ACGEXS_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAV1978 family virulence-associated passenger
                     protein"
                     /protein_id="HFF8011333.1"
                     /translation="MTDNARKEYLNQFFGSKRYLYQDNERVAHIHVANGNYYFHGHIV
                     PGWQGVKKTFDTAEELEIYIKQHGLEYEKQKQLTLF"
     gene            10908..11156
                     /locus_tag="ACGEXS_000285"
     CDS             10908..11156
                     /locus_tag="ACGEXS_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HFF8011334.1"
                     /translation="MNNREQIEQSVISASAYNGNDTEGLLKEIEDVYKKAQAFDEILK
                     GLPNAMQDALKEDIGLDEAVGIMTGQVVYKYEEAQENE"
     gene            11149..11685
                     /locus_tag="ACGEXS_000286"
     CDS             11149..11685
                     /locus_tag="ACGEXS_000286"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFF8011335.1"
                     /translation="MNNTLTIDQLQELLQIQKEFDDRIPTLNLRDSKIAYVVEFFEWF
                     NTLETFKNWKKKPGKPLDVQLDELADMLAFGLSIANQVGVSSEEIKEAIESSFKDTEF
                     HKMFNFKDKEFAQDAVVSTPQIIFKEFYPDQQAIVIVIDIAYNLYSIDQLIDAYKKKM
                     KRNHERQDGTADAGKGYV"
     gene            11722..11967
                     /locus_tag="ACGEXS_000287"
     CDS             11722..11967
                     /locus_tag="ACGEXS_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011336.1"
                     /translation="MSDMLEIFLIGFGVYLFYRIAIIFLKSKKTIHTNIYEMLMLATI
                     FMISTFAYKHQKTHILIAFLVMFFMSKLKQVQGSYEE"
     gene            11964..12170
                     /locus_tag="ACGEXS_000288"
     CDS             11964..12170
                     /locus_tag="ACGEXS_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HFF8011337.1"
                     /translation="MTQYLVTTFKDSTGRKHTHITKAKSNQRFTVVEAESKEEAKEKY
                     EKQVKRDAVIKVGQLFENIRECGK"
     gene            12167..12553
                     /locus_tag="ACGEXS_000289"
     CDS             12167..12553
                     /locus_tag="ACGEXS_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011338.1"
                     /translation="MIKKLKNMDGFDIFIVGILSLFGITALLLVVALPIYTVASYQNK
                     EVHQGTITDKYNKRQDKEDKFYIVLDDKQVIENSDLFFKGKFDSADIQARLKVGDKVK
                     VKTIGYRIHFLNLYPVLYEVKKVDKK"
     gene            12550..12699
                     /locus_tag="ACGEXS_000290"
     CDS             12550..12699
                     /locus_tag="ACGEXS_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFF8011339.1"
                     /translation="MIKQILRLLFLLAMYELGKYVTEQVYIMMTANDDVEAPSDYVFR
                     AEVSE"
     gene            12699..12899
                     /locus_tag="ACGEXS_000291"
     CDS             12699..12899
                     /locus_tag="ACGEXS_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1514 family protein"
                     /protein_id="HFF8011340.1"
                     /translation="MWITMTIVFAILLLVCISINSDRAREIQALRYMNDYLLDEVVKT
                     KGYNGLKEYRIELKRMNNDIKK"
     gene            12927..13343
                     /locus_tag="ACGEXS_000292"
     CDS             12927..13343
                     /locus_tag="ACGEXS_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFF8011341.1"
                     /translation="MIKIEKHDIKKLEEYIQHIDNYRRELKMREYELLESHEPDNAGA
                     GKSNLPGNPIERCAIKKFSDNRYNTLRNIVNGVDRLIDESDEDTLELLRFRYWDCPIG
                     CYEWEDIAHYFGTSKTSILRRRNALIDKLAKYIGYV"
     gene            13575..13874
                     /locus_tag="ACGEXS_000293"
     CDS             13575..13874
                     /locus_tag="ACGEXS_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFF8011342.1"
                     /translation="MMTKDERIRFYKSKEWQTTRKRVLERDNYECQQCKRDGKLTTYD
                     KSKRKSLDVDHILSLEHHPEFAHDLNNLETLCIKCHNKKEKRFIKKENKWKDEKW"
     gene            14004..14348
                     /locus_tag="ACGEXS_000294"
     CDS             14004..14348
                     /locus_tag="ACGEXS_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011343.1"
                     /translation="MEIIVDENLVLKEKERLQVLYKDIPSNKLKVVDGLIIQAARLRV
                     MLDYMWEDIKEKGDYDLFTQSEKAPPYERERPVAKLFNARDAAYQKIIKQLSDLLPEE
                     KEDTETPSDDYL"
     gene            14345..16006
                     /locus_tag="ACGEXS_000295"
     CDS             14345..16006
                     /locus_tag="ACGEXS_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFF8011344.1"
                     /translation="MISNKYVDEYINLWKQGKIILNKERIDLFNYLQKHIYSRDDVYF
                     DEQKIEDCIKFIEKWYFPTLPFQRFIIANIFLIDKNTDEAFFTEFAIFMGRGGGKNGL
                     ISAISDFLSTPLHGVKEYHISIVANSEDQAKTSFDEIRTVLMDNKRNKTGKTPKAPYE
                     VSKAKIINRATKSVIRYNTSNTKTKDGGREGCVIFDEIHYFFGPEMVNVKRGGLGKKK
                     NRRTFYISTDGFVREGYIDAMKHKIASVLSGKVKNSRLFAFYCKLDDPKEVDDRQTWE
                     KANPMLHKPLSEYAKTLLSTIEEEYNDLPFNRSNKPEFMTKRMNLPEVDLEKVIAPWK
                     EILATNREIPNLDNQMCIGGLDFANIRDFASVGLLFRKNDDYIWLGHSFVRQGFLDDV
                     KLEPPIKEWEKMGLLTIVDDDVIEIEYIVDWFLKAREKYGLEKVIADNYRTDIVRRAF
                     EDAGIKLEVLRNPKAIHGLLAPRIDTMFAKHNVIYGDNPLMRWFTNNVAVKVKPDGNK
                     EYIKKDENRRKTDGFMAFVHALYRADDIVDKDMSKALDALMSIDF"
     gene            16022..17209
                     /locus_tag="ACGEXS_000296"
     CDS             16022..17209
                     /locus_tag="ACGEXS_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFF8011345.1"
                     /translation="MSILEKIFKTRKDITYMLDLDMIEDLSQQAYVKRLAIDSCIEFV
                     ARAVAQSHFKVLEGNRIQKNDVYYKLNIKPNTDLSSDSFWQQVIYKLIYDNEVLIVVS
                     DSKELLIADSFYREEYALYDDIFKDVTVKDYTYQRTFTMQEVIYLKYNNNKVTHFVES
                     LFEDYGKIFGRMIGAQLKNYQIRGILKSASSAYDEKNIEKLQAFTNKLFNTFNKNQLA
                     IAPLIEGFDYEELSNGGKNSNMPFSELSELMRDAIKNVALMIGIPPGLIYGETADLEK
                     NTLVFEKFCLTPLLKKIQNELNAKLITQSMYLKDTRIEIVGVNKKDPLQYAEAIDKLV
                     SSGSFTRNEVRIMLGEEPSDNPELDEYLITKNYEKANSGENDEKEKDENTLKGGDEDE
                     SGD"
     gene            17193..17930
                     /locus_tag="ACGEXS_000297"
     CDS             17193..17930
                     /locus_tag="ACGEXS_000297"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFF8011346.1"
                     /translation="MKAEIKGVIVSNEDKWVYEMLGMDSTCPKDVLTQLEFSDEDVDI
                     IINSNGGNLVAGSEIYTHLRAHKGKVNVRITAIAASAASLIAMAGDHIEMSPVARMMI
                     HNPSSIAQGEAKDLNHAAETLEHVGQIMAEAYAVRAGKNKQELVEMMARETWLNADEA
                     IEQGFADSKMFENDNMQIVANNTQVLSKDVLNRVTALVSKTPEVNIDIDAIANKVIEK
                     INMKEKESEIDVADSKVSANGFSRFLF"
     gene            17954..19099
                     /locus_tag="ACGEXS_000298"
     CDS             17954..19099
                     /locus_tag="ACGEXS_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFF8011347.1"
                     /translation="MTINLSETFANAKNEFINAVNNGEPQERQNELYGDMINQLFEET
                     KLQAKAEAERVSSLPKSAQSLSANQRSFFMDINKNVNYKEEKLLPEETIDRIFEDLTT
                     NHPLLADLGIKNAGLRLKFLKSETSGVAVWGKIYGEIKGQLDAAFSEETAIQNKLTAF
                     VVLPKDLNDFGPAWIERFVRVQIEEAFAVALETAFLKGTGKDQPIGLNRQVQKGVSVT
                     EGAYPEKEEQGTLTFANPRATVNELTQVFKYHSTNEKGKSVAVKGNVTMVVNPSDAFE
                     VQAQYTHLNANGVYVTALPFNLNVIESTVQEAGKVLTYVKGLYDGYLAGGINVQKFKE
                     TLALDDMDLYTAKQFAYGKAKDNKVAAVWKLDLKGHKPALEGTEETL"
     gene            19119..19403
                     /locus_tag="ACGEXS_000299"
     CDS             19119..19403
                     /locus_tag="ACGEXS_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011348.1"
                     /translation="MVKFKVVRAFKDIEHNQHKYKVGELYPAEGYNNPRVELLTNQIK
                     NKYDKVYIVPLDKLTKQELLELCESLQKKASSSMVKSEIVDLLNGEDNDD"
     gene            19393..19677
                     /locus_tag="ACGEXS_000300"
     CDS             19393..19677
                     /locus_tag="ACGEXS_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009560502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HFF8011349.1"
                     /translation="MTIDDLLVKFKSLEKIDHNSEDEYLKQLLKMSYERIKNQCGVFE
                     LENLIGQELILIRARYAYQDLLEHFNDNYRPEIIDFSLSLMEVSEDEESV"
     gene            19661..20023
                     /locus_tag="ACGEXS_000301"
     CDS             19661..20023
                     /locus_tag="ACGEXS_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HFF8011350.1"
                     /translation="MKKVFKKPRITTKRLNTRVHFYKYTENNGPEAGEKEEKLLYSCW
                     ASIDGVWLRELEQAISNGTQNDIKLYIRDPQGDYLPSEEHYLEIESRYFKNRLNIKQV
                     SPDLDNKDFIMIRGGYSS"
     gene            20020..20424
                     /locus_tag="ACGEXS_000302"
     CDS             20020..20424
                     /locus_tag="ACGEXS_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFF8011351.1"
                     /translation="MSVKVIGDKALERELEKRFGIKEMVKVQDKALIAGAKVIVEEVK
                     KQLKPSKDTGALINEVSFSKPEWINGKRTITVHWRGSKDRYKIVHLIEYGHVQKGTGK
                     FIKPKAMGGVNRAIRQGQNKYFETLKRELKKL"
     gene            20421..20828
                     /locus_tag="ACGEXS_000303"
     CDS             20421..20828
                     /locus_tag="ACGEXS_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011352.1"
                     /translation="MIDILYKVHEVISQDRIIREHVNINNIKFNKYPNVKDTDVPFIV
                     IDDIDDPIPTTYTDGDECAYSYIVQIDVFVKYNDEYNARIIRNKISNRIQKLLWSELK
                     MGNVSNGKPEYIEEFKTYRSSRVYEGIFYKEEN"
     gene            20829..21473
                     /locus_tag="ACGEXS_000304"
     CDS             20829..21473
                     /locus_tag="ACGEXS_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFF8011353.1"
                     /translation="MAVKHASAPKAYINITGLGFAKLTKEGAELKYSDITKTRGLQKI
                     GVETGGDLKTAYADGGPIESGNTDGEGKISLQMHAFPKEIRKIVFNEEYDEDGVYKET
                     QGKQNNYVAIWFRQERRDGTFRTVLLPKVMFTNPKIDGETAEKDWDFSSEEVEGEALF
                     PLVDNKKSVRKYIFDSANMTNHDGDGEKGEEAFLKKILGEEYTGNVTEGNEETL"
     gene            21509..21739
                     /locus_tag="ACGEXS_000305"
                     /pseudo
     CDS             21509..21739
                     /locus_tag="ACGEXS_000305"
                     /inference="COORDINATES: protein motif:HMM:NF014427.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            21789..22139
                     /locus_tag="ACGEXS_000306"
     CDS             21789..22139
                     /locus_tag="ACGEXS_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011354.1"
                     /translation="MAKLKRNIIQLVEDPKANEIKLQTYLTPHFISFEIVYEAMDLID
                     DIEDENSTMKPREIADRLMDMVVKIYDNQFTVKDLKERMHAPDGMNALREQVIFITQG
                     QQTEETRNFIQNMK"
     gene            22193..22327
                     /locus_tag="ACGEXS_000307"
     CDS             22193..22327
                     /locus_tag="ACGEXS_000307"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011355.1"
                     /translation="MDLIENGKDANEVLKMPFHYVLSIYQNKNNDISEEKAEALIDAF
                     "
     gene            22384..26928
                     /locus_tag="ACGEXS_000308"
     CDS             22384..26928
                     /locus_tag="ACGEXS_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001799555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFF8011356.1"
                     /translation="MGERIKGLSIGLDLDAANLNRSFAEIKRNFKTLNSDLKLTGNNF
                     KYTEKSTHSYKQRIKELDGTITGYKKNVDDLAKQYGKVSQEQGENSAEAQKLRQEYNK
                     QANELNFLEKELEKTTTEFEEFKKAQVEAQRMAESGWGKTSKVFESMGPKLTKMGDGL
                     KSIGKGLMIGVTAPVLGIAAASGKAFAEVDKGLDTVTQATGATGGELKKLQNSFKDVY
                     GNFPADAETVGGVLGEVNTRLGFTGKELESATESFLKFSHITGSDGVQAVQLITRAMG
                     DAGIEADEYQSVLDMVAKAAQASGISVDTLADSITKYGAPMRAMGFEMKESIALFSQW
                     EKSGVNTEIAFSGLKKAISNWGKAGKDPREEFKKTLAEIERTPDIASATSLAIEAFGA
                     KAGPDLADAIKGGRFSYQEFLKTIEDSQGTVNQTFKDSESGSERFKVAMNKLKLVGAD
                     VWASIESAFAPVMEELIKKLSVAVDWFSSLSDGSKRSIVIFGGIAAAIGPVVFGLGAF
                     ISTIGNAVTVLAPLLASIVKAGGLISFLSTKVPILGTVFTALTGPIGIVLGVLAGLAV
                     AFTIAYKKSETFRNFVNGAINSVKQTFSNFIQFIQPFIDSVKNVFKQAVSAIVDFAKD
                     IWSQINGFFNENGISIVQALQNICNFIKAIFEFILNFVIKPIMFAIWQVMQFIWPAVK
                     ALIVSTWENIKGVIQGALNIILGFIKFFSSLFTGNWRGVWDGIVMILKGTVQLIWNLI
                     QLWFVGKILGVVRYFGGLLKGLISGIWGVIKGIFTKSLSAIWNATKSIFGFLYNSVKS
                     IFTNMKNWLSSTWNNIKSNTVGKAHSLFTGVRSKFTSLWNATKDIFTKLRNWMSNIWN
                     SIKDNTVGIAGRLWDRVRNIFGSMRDGLKSIISKIKDHIGGMVDAVKRGLNKLIEGLN
                     WVGGKLGMDKIPKLHTGTEHTHTTTRLVKNGKIARDTFATVGDKGRGNGPNGFRNEMI
                     EFPNGKRVITPNTDTTAYLPKGSKVYNGAQTYSMLNGTLPRFHFGTTMWKDIKSSASS
                     AFNWTKDQIGKGTKWLGDKVGDVMDFIDNPGKLLNYVLKAFGVDFSSLTKGMGIVGDI
                     TKASWNKIKSKAINWIKEGLESQAGDGSVFDSFRILQPYSAPPKPPNPNYPFNGGVHH
                     GVDYDTPTGTPIRTPMGGRVRSWYDNYGGGKAITVQKGRTFLWFMHLSEQLRRTGEQI
                     KAGQLIGKSGNTGSMTNYRHLHFQVNQGGESNRYSTDPIPWLRKNDKTGGKNSPGGSG
                     SENARRAIRTAQNILGGQYKASWITHEMMRVARRESNYTANAVNNWDSNARAGTPSRG
                     MFQMIDPSFRAYAKSGYNNPLNPTHQAISAMRYIVGKWVPRTGSWRAAFKRAGDYAYA
                     TGGKVYNGLYHLGEEGYPEWVIPTDPARKNEAMKMLHYAAAEVRGRKASKNKRPSQLS
                     SVNGFDDPSLLLKMIEQQQQQIALLLKIAQSNDVIADKDYQPIIDEYAFDKKVNASIE
                     KRERQESTKVKFRKGGIAIQ"
     gene            26925..28409
                     /locus_tag="ACGEXS_000309"
     CDS             26925..28409
                     /locus_tag="ACGEXS_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail domain-containing protein"
                     /protein_id="HFF8011357.1"
                     /translation="MIDTIKVNNKTIPWLYVERGFEIPSFNYVLKTENVDGRSGSIYK
                     GRRLESYSFDIPLVVRNDYLSHNGFKTHDDVLNELVKFFNYEEQVKLQFKSKDWYWNA
                     YFEGPIKLHKEFAIPVKFTIKVVLTDPYKYSVTGYKNTAISDQVSVVNSGTADTPLIV
                     EARAIKPSSYFMITKNDEDYFMVGDDEVTKEVKDYMPPVYHSEFRDFKGWTKMITEDI
                     PSNDLGGKVGGDFVISNLGEGYKATNFPDAKGWVGAGTKRGLPKAMTDFQITYKCIVE
                     QKGKGAGRTAQHIYDSDGKLLASIGYENKYHDRKIGHIVVTLYNQKGDPKKIYDYQNK
                     PIMYNLDRIVVYMRLRRVGNKFSIKTWKFDHIKDPDRRKPIDMDEKEWIDGGKFYQRP
                     ASIIAIYSAKYNGYKWMEMNGLGSFNTEILPKPKGARDVIIQKGDLVKIDMQAKSVVI
                     NEEPMLSEKSFGSNYFNVDSGYSELIIQPENVFDTTVKWQDRYL"
     gene            28425..32210
                     /locus_tag="ACGEXS_000310"
     CDS             28425..32210
                     /locus_tag="ACGEXS_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFF8011358.1"
                     /translation="MIHVLDFNDKIIDFLSTDDPSLVRAIHKRNVNDNSEMLELLISS
                     ERAEKFRERHRVIIRDSNKQWREFIINWVQDTMDGYTEIECIASYLADITTAKPYAPG
                     KFEKKTTSEALKDVLSDTGWEVSEQTEYDGLRTTSWTSYQTRYEVLKQLCTTYKMVLD
                     FYIELSSNTVKGRYVVLKKKNSLFKGKEIEYGKDLVGLTRKIDMSEIKTALIAVGPEN
                     DKGKRLELVVTDDEAQSQFNLPTRYIWGIYEPQSDDQNMNETRLRSLAKTELNKRKSA
                     VMSYEITSTDLEVTYPHEIISIGDTVRVKHRDFNPPLYVEAEVIAEEYNIISENSTYT
                     FGQPKEFKESELREEFNKRLNIIHQKLNDNISNINTIVKDVVDGELEYFEHKIHKSDT
                     PPENPVNDMLWYDTSNPDVAVLRRYWNGRWIEATPNDVEKLGGITREKALFSELNNIF
                     INLSIQHASLLSEATELLNSEYLVDNDLKEDLQASLDAVIDVYNQIKNNLESMTPETA
                     TIGRLVDTQALFLEYRKKLQDVYTDVEDVKMAISDRFKLLQSQYTDEKYKEALEIIAT
                     KFGLTVNEDLQLVGEPNVVKSAIEAARESTKEQLRDYVKTSDYKIDKDGIVERLDTAE
                     AERTTLKGEIKDKVTLNEYRNGLEEQKQYTDDQLSDLSNNPEIKASIEQANQEAQEAL
                     KSYIDAQDDLKEKESQAYADGKISEEEQRAIQDAQAKLEEAKQNAELKARNAEKKANA
                     YTDNKVKESTDAQRKTLTRYGSQIIQNGKEIKLRTTKEEFNATNRTLSNILNEIVQNV
                     TDGTTIRYDDNGVAQALNVGPRGIRLNADKIDINGNREINLLIQNMRDKVDKTDIVNS
                     LNLSREGLDINVNRIGIKGGDNNRYVQIQNDSIELGGIVQRTWRGKRSTDDIFTRLKD
                     GHLRFRNNTAGGSLYMSHFGISTYIDGEGEDGGSSGTIQWWDKTYSDSGMNGITINSY
                     GGVVALTSDNNRVVLESYASSNIKSKQAPVYLYPNTDKVPGLNRFAFTLSNADNAYSS
                     DGYIMFGSDENYDYGAGIRFSKERNKGLVQIVNGRYATGGDTTIEAGYGKFNMLKRRD
                     GNRYIHIQSTDLLSVGSDDAGDRIASNSIYRRTYSATANLHITSAGTIGRSTSARKYK
                     LSIENQYNDRDEQLEHSKAILNLPIRTWFDKAESEILARELREDRKLSEDTYKLDRYV
                     GLIAEEVENLGLKEFVTYDDKGEIEGIAYDRLWIHLIPVIKEQQLRIKKLEESKNEG"
     gene            32200..32352
                     /locus_tag="ACGEXS_000311"
     CDS             32200..32352
                     /locus_tag="ACGEXS_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011359.1"
                     /translation="MKDNKQGLQANPEYTIHYLSQEIMRLTQENAMLKAYIQENKENQ
                     QCAEEE"
     gene            32399..32686
                     /locus_tag="ACGEXS_000312"
     CDS             32399..32686
                     /locus_tag="ACGEXS_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011360.1"
                     /translation="MAKEIINNTERFILVQIDKEGTERVVYQDFTGSFTTSDSASYAQ
                     DFKSEENAKKIAETLNLLYQLTGNQNGVKVVKEVVDRTDLSSDKSVDSETM"
     gene            32742..33116
                     /locus_tag="ACGEXS_000313"
     CDS             32742..33116
                     /locus_tag="ACGEXS_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011361.1"
                     /translation="MDDIQKIKKELSELVERVDDVEILANETADHVLELREEHKQHHN
                     ELRESHKELKDKQDKVVDENLEQTKILNRIEERYQTQVDVAQKNEEKTLAQNKWLVGA
                     IWALVTIVMIAVITASITALLP"
BASE COUNT        12733 a         4799 c         6608 g         9252 t
ORIGIN      
        1 aggaggatta atataagatg aataaacttt tactattgat aaatccttaa ttaaagatac
       61 agcaacttcg caagggctgt tcatatgttc attcatagtt tcaaagtcta agactgcaat
      121 atcatatttt ttcatttgca agtgctcctt ttataaaata acttttccaa ttaacctcac
      181 actttcattt ctataaaagt gtagatcgtc gtaatcttta tttagtgaaa ctagagtcaa
      241 tctatcatct tcaacaaaga ctttcttaac gtacgcttct tcttcaatga tgaatatacc
      301 aatttgtcca ttctttatat tgtgagtttt ctccacaaat atgatttcgc catctttaaa
      361 cataggttcc atagaatcac catttacttt taacgctaaa tcgtgtgtgg ggataggtcc
      421 tttaaccatt tcagtaaata gcgtttcatc gtgtaaacgt tctcctacac cagcagagac
      481 gcaaccattg acgttaactg gagttttctc ctgtttatat gaattaatat ctacaacgtt
      541 atctccttta gaattctgtt cttccaattg ttcatttgca tagttaagta cgttttcttg
      601 gcggggaggt gtgagtttgt tgtatatgga agtgatgtcg ttatcgtctt tgtatgtagt
      661 atctatgtcg cttttaccaa cctcgaaaac atcagctatc ctttgtataa cgccgtgaga
      721 ggggttggaa cgtaaattta aataatcgct taaagtagat ggttttatgt taatgagttc
      781 agcaagtttc ttttgagaca tatttgaatc gttgagaaat tttctaatgt ttttggctat
      841 aataatattt ctttctttgt tcatattact tacctccttt tttcttatta tacgaaattt
      901 tcatatcata gtaaagtttt ttacgaaaaa aacgtattta atgttgacaa tacgaaaatt
      961 tcgtattata ttaggtttac gaaaggcggt gacaacatga aaacattaaa agagttgagg
     1021 actgattacg gattgactca aaaagagtta ggagatttat ttaaggtctc atcacgtaca
     1081 attcaaaata tggaaaaaga ctctacaaac attaaagata gtttactttc taagtatatg
     1141 agtgctttta atgttaaata tgatgatatt tttttaggta atgaatacga aaatttcgta
     1201 tttacgaatg ataaaaagaa atcaattatt ttagcattta aagaaaaaca aacatcttaa
     1261 taggaggaat aacaaatgaa cattcaagaa gcaacgaagc tagcgatgga gaaaggaata
     1321 agtataagga gagagaatca agatgtgtat gggatattac caactaattt gcagcgttat
     1381 caatgcctag tcgtatctag acactataag aaaaaaagac aaaccgccgc cggaaggtgg
     1441 cagcctagcg cagacgattt aatagcagat gattggattt tagattatta attttttcaa
     1501 atctctaatt aaacccataa gtgttttgta atcttttttg gattctgatt ctgagtaggc
     1561 gataccttct cgagaaagag ccatctcaag aaaaccgcca tcttcagcag aagcaattac
     1621 aaaatctcta tgctttaatt caagaactgc atcgatatag tcttcaaaat taaaacctaa
     1681 aaagaaagcg ttaaatgagg attcatcact accgaaataa gatgcagaac gtttagacat
     1741 accttcgtca attctatcaa ggtaaattga ataaagttgt aaaaggacaa atttagcttc
     1801 atcagtcata agtcattcac ctccttaata ggagtatagc agaaaggagc acaaacaata
     1861 tgcaagcatt acaaacaaaa tcgaacatag gcgaaatgtt caacatacaa gaaaaagaaa
     1921 atggagaaat cgcaatcagc ggtcgagaac ttcatcaagc attagaggtt aagactccat
     1981 acaaaaaatg gtttgaaaga atgagtgatt acggatttga agaaaatatc gattatatag
     2041 tcacggacat ttttgtccat aacccactag gaggtcgtca gaatcaaact gaccacgcac
     2101 tcacactaga cactgcaaaa gagattgcaa tgattcaacg tagtgaacct ggcaaacgtg
     2161 caagacaata cttcatccaa gttgaaaaag catggaatag cccagaaatg attatgcaac
     2221 gtgctttaaa aattgctaac aacacaatca atcaattaga aacaaagatt gaacgtgata
     2281 aaccaaaaat tgtatttgca gatgcagtag ctactactaa gacatcaatt ttagttggag
     2341 agttagcaaa gatcattaaa caaaacggta taaacatcgg gcaacgcaga ttgtttgagt
     2401 ggttacgtca aaacggattc cttattaaac gcaagggtgt ggattataac atgcctacac
     2461 agtattcaat ggaacgtgag ttattcgaaa ttaaagaaac atcaatcaca cattcggacg
     2521 gtcacacatc aattagtaag acgccaaaag taacaggcaa aggacaacaa tactttgtta
     2581 ataagttttt aggagaaaaa caaacatctt aataggagga acgaacaatg caagctcaaa
     2641 acaaaaaagt catctattac tactatgacg aagaaggtaa tagacgaccc gttaatattc
     2701 aatacaacga tggctacgac ttaatgatag accagcgttt tattgaaatg acgcttgaaa
     2761 gacatccgca tttaaaaaat aacttttatg gattaataga tggaaaagaa tttaagttag
     2821 attaaatttt tgtgttagat aattaaaagc taatttgctt agcaatgtta cggacatact
     2881 agtggttttg tttgcgactt ttttaacttc tttccaagtg tgattgtctc ggatattatc
     2941 taaaaattca tgccctgacc aagttatatc gttaattgta taaccataaa tatgtccatc
     3001 ttcccaaccg aatttaacac taacatactt tgcttcttcc agttttaata atgcatacat
     3061 tacagtttca aaatcatatt ttccaaatac aacattatct ttgaaattgt attcggtgag
     3121 cggttcacca atctttttat tagtttcaat ttctaacaaa agatgtctaa cacaatcatg
     3181 atctaatttc atacttatca ctactttagg ttgataacaa cattatacac gaaaggaaag
     3241 atagaaatgc cacatatttt aaacgtaaca gttccaatac ctgaaacaca cgtgcttatc
     3301 acaaaagatg aatatgaaga gttaatagct tactcattag accctgtatg gaacatgagc
     3361 gacttaaaga agaaattaaa aattgcatct gatgaaacaa tcaaagacag gttattattt
     3421 catcctagac tcgaaaaaga gttaagagca caaggtatcg tacattatcc tgatgagaat
     3481 tttaatcgtt ggaggtttaa cgcaagaagg atgcataagt ttgtagatga acattttaat
     3541 gagatttaca aaggagggca caacaaatga gtaaaactta taaaagctac ctagtagcag
     3601 tactatgctt cacagtctta gcgattgtac ttatgccgtt tctatacttc actacagcat
     3661 ggtcaattgc aggattcgca agtatcgcaa cattcatatt ctataaggaa tacttttatg
     3721 aagaataaaa aaactgctac ttgcgccaac aagtaacagt aacaaacatt taagaaataa
     3781 aattcaagtt aaatataaaa cgaaaaacgg aggaagtcaa ccatgactaa aaattataaa
     3841 gacatgacgc aggaagaaat aaaagactta ttatctgaaa aaacggcaga attgtatgaa
     3901 ttagcgaaag aaattaaggg agaaagtaaa tttgatattt tgcttttctc atcaatagga
     3961 gttatcgacg gagattattt agcaggttca agttctgtga ttggtcatac ttttgatctt
     4021 gcttacttat tggatagcac taagagttat aaagatattg tcaatgttct ccaaatgtgt
     4081 aaatcacaaa aaattctcgg tatagatgac gacaaggagg actaaaacaa tgtattacaa
     4141 aacgggtgac gtatgtcgaa aaatatttaa tgtagatggc tttgattttc aattaagagt
     4201 taagaagcga gcatatagtg tcgaaatagt cgttttagat catgaaggaa attcaattga
     4261 cgggctacta gtttctgacg agaacgatct atacacagct ttagatattt tgaaacaaag
     4321 tatttatgaa tggattgaaa ataacacaga tgaacaggac agactaatta acttagtcat
     4381 gaaatggtag atataagcat gagagataca gaaagaaata tattgaatat ttttaagacg
     4441 ttattcgacg aatatacttt gtcaaaccaa cgagcattat tggaaattga acgtaatcat
     4501 cacggatact tatcgattaa tttcttgcac tatcacgaca gttacaaaac aaacaataag
     4561 cttgtgcaga tacatgaaat caatccagac agccatgaac gaataaaaaa tttaattatc
     4621 gaggtgctaa gaggtcatcg gaagattaaa aaaggagcat gaggaaagat atgaaaataa
     4681 ataagttaac tatatcgaac tttgctggaa tcaaagaaga aaaatttaac tttgacggta
     4741 aagatgcaaa aatatacggc aataatgcga ctggcaagac tacaacagca accgcattac
     4801 aatggctgct tttcgataag ggtttagacg gttcaaccaa atcatttaac cctgtacctt
     4861 taaacgaaaa aaacgaagaa aattatgagt taattccgac tgttttcgca gaatttgaaa
     4921 tcgacggaaa aattacgact tttaaaaaag agtcacatcc taaatacaca ataaatcaaa
     4981 aaacgaatcg caaggaatac tcacgaagtc gaacgaagaa acaatatatc aatgatgaat
     5041 caataaaagt aaaggattat aaagctcgta ttgatgaact gattgatgaa gatgtattca
     5101 agttaattac gaaccctcaa gcatttaact tactagattg gaagaaacga agaagtttgt
     5161 tgtttgaaat cgctaaacca atcaatgatg aggatgtcat taaaacaaat gatgatttta
     5221 aagaactaaa taatattctt ggagatcacg aaattgaaac aaagaaaaag attcttacag
     5281 acaagataaa acagattaac aaagatatca aagatattcc gatacgtatt aaccaaacgc
     5341 aacaaaataa gcaggatgta ccggaattcg ataatgatag acacacaatc ataaaacaag
     5401 aaattgagca acttgaaaat gagcgtatag atattcaaaa cggtgcagaa gaaattaatt
     5461 tgcgtaacca attagctgat aaacaatcag aattgaagcg catagaagct aataatagcg
     5521 ccagtaatga gaacaaaata catgctttaa caaatgagct acacgttgaa aatggaacgg
     5581 ttgcgaatct taaaacaaga ttaaagcaaa acaaacaaca aattacacat gaagaaaatc
     5641 gacgtaatca attattagaa aatcataaag gattaaaaag tgatttagaa aaagctaaaa
     5701 atcaaaaatt tgaatatctt gatgacaatg tatgtagttg ttgtggtcaa cagttaccag
     5761 ctgaacaagt gagtgaggta agagaaaaag cattgcagaa attcaatgca aacaaatcga
     5821 aagaattaga aacaatacaa acatctatca atcacattat ttcagagggc aagaaaataa
     5881 agccaattat cgagaaatta gaggatgaca acaataattt acaaattaaa atcaacgaag
     5941 cagaagagcg ttcagcaaga atacaaaaca aaattaataa gttgaaaaca actcacgttg
     6001 acgttacgca aactgacgaa tacaaagcag taatgttaga gataaatgag attaatcaaa
     6061 aacgctctaa catcaggaaa actattcaag ataaagtttc aggaatagat gacaaaataa
     6121 gcgaacttac tcaagaaaaa tcagaaattg aagtgtcaat atcaatcgaa aaatcaaata
     6181 aacatctaga tgatgttatt tctgaattaa gaaatgaaga agacagatta ttggatgaaa
     6241 aagaaaagta ttcacatgac ctttatatct taaaagaatt tacaacaaca aaagtcaaaa
     6301 tgcttactga aaacatcaat aacgaatttg atattgctga atttaagcta ttcaatacct
     6361 tagttaacgg cgaattagaa gaaacatgtt caacaacggt taatggtgtc gagtatgaca
     6421 gcggtttaaa taacgcctca agaattaatg ttggcttaga tatcatcaac acactatcaa
     6481 aacattttaa agttacagcg ccaatattta ttgataatgc tgaatcagta acagagctta
     6541 tcaaaacaga atcacaacaa attcaattga tagtaaatga acaagataaa aaattaagaa
     6601 tggagactat ataaaatgac tgaaaataat aaattacaaa ctattgaaca acaattagta
     6661 caagaaaaga acgtatctga caacgtatta aacaaagtga gagttttaga gtcacaaggc
     6721 aatttggaat tgccaaatga ttattcacca agtaatgcca tgaaacaagc atggttacaa
     6781 atcagccaag ataacaaatt aatgagttgt aacgatacaa gcaaagcaaa tgccttatta
     6841 gacatggtaa cgcaaggttt aaatccagct aaaaatcaat gctactttat tccttacggc
     6901 aacaaaatgc agttacaacg tagctatcac ggtaatgtaa tgatgttaaa acgtgatgca
     6961 ggtgctcaag atgttgttgc tcaagtgatt tataaaggcg atacattcaa gcaagaaatg
     7021 ggagaaacag gacgtatcaa agcgattaaa cacgaacaag acttctttaa catcgacaaa
     7081 gaaaacatta tcggtgcgta ctgcacaatc gtatttaatg atggacgaga taactatatt
     7141 gaagtcatga ctattgaaca aattaaacaa gcatggatgc agtcatcaat gattaaagat
     7201 gaaaaagcat tacaaaattc taaaacacat aataatttca aagaagaaat ggctaaaaaa
     7261 acagttatca atagagctgc taaacgttat atcaacacat caacagatag caatcttttc
     7321 aaatacgcac aagaatccga acaacgtcaa cgcaaagaag tgttggacgc agaagttgaa
     7381 gaaaatgcaa atcaagaaca attggacttt gaacaaccag ttcttgaaga agcacaatac
     7441 acagaattag aaaatgataa gcctattgat gtatctgact ttgaagaaat aaaagaacct
     7501 gcaacagaaa aagaaagcga agaagagcca ttttaattga aacaatagca actggttcaa
     7561 gtggtaactg ctacgtctta aatgatggac gtactacgtt actatttgag gcaggtataa
     7621 aatttgaacg tgttcaaaag catttcaaat ataaaacaag acatatagca gggtgtctta
     7681 tcacacacga acatggtgat catgcaaagt atacaaagca gtttgtcgac aatggtgtaa
     7741 tcagctatat gactgctgga acacaacaag ctatgaattt tgaaagtcat cgcttatgca
     7801 cgattaaggc aaagcaagag ctgcgaatag gcacatggtc aattctaccg tttgacatcg
     7861 aacatgatgc taacgagcct gtggctttct tattacaaag cacactaggt tataaggttc
     7921 tgtatgttac tgatacaaag tatttgaaat acaaatttaa tggcattacg cacatgatgc
     7981 tagaagttaa ttatatctat gaacaaatgc aagaaaacat aaaaaacggc agtgtgcaca
     8041 gcacattagc aaacagaatt atggagtctc attttagctt agaacatgct atcggaatgt
     8101 taaaagcaaa tgatttaact agactcgaag aaatacattt aattcattta agtagtcaaa
     8161 attcaaatgc aaaatacatt aaaagtgaaa tacaaaaagt gacgggcgcg cccgtttatt
     8221 ttggaggttt ataaatgcta aacagaacaa tattagttgg tcgtttaact agagacccag
     8281 aattaagaac cactcaaagt ggtgtaaatg tagcatcatt cacattagca gttaaccgta
     8341 catttacaaa tgcacaaggc gagcgcgagg cagactttat taatatcatc gtatttaaaa
     8401 aacaagcaga gaacgttaat aaatacctat ctaaaggatc gttggcgggc gtagatggta
     8461 ggttacaaac gcggaactat gaaaataagg aaggtcaacg tgtatacgtt acggaagtta
     8521 ttgctgatag tattcaattt ttagaaccga aaaactcaaa tgacactcaa caagatttat
     8581 atcaacaaca agtacaacaa acacgtggac aatcgcaata ttcaaataac aaaccagtaa
     8641 aagataatcc gtttgcgaat gcaaatggtc cgattgaact aaatgatgat gatttaccat
     8701 tctaatttaa ccggtttgaa agtgaggtgt gtatatgact ggttggataa gtattgatcg
     8761 ctcaattcaa aatcattggc tatttaaaga aaagagaaca ttttcaaagt tcgaagcatg
     8821 gatatattta ctcatggaag cgaatcattc agaggcaaaa gtgcctatag gaaaccaaat
     8881 tgtaaccgtt gaaagaggac aaagattaac atctattttg accttgtctg acctttttaa
     8941 ctggtcacga tttaaagtga aaaccttcct tgacttactc gagagtgatg gaatgttaga
     9001 agtcaaaaca acatcaaaat atacccttat aaccattgtc aattatgact tttatcaaag
     9061 tgagcagggc aggaaccaac atcaaaacga catcaaacca acatcaaaac aacatcagtc
     9121 aaacatcaac ccaacatcaa aacaacatca aaccaacaca aacaataatg ataataaaga
     9181 taataatgaa aagaatgtga ataatgagaa gaagaaggta accgccttcg acttcttcca
     9241 agataacgga ttcggtttca taactcctta caatttagac gatttaaatt actatcttga
     9301 ttcatttgaa aatgattcag atgaaatagt taccgcatca cttaaaatcg ctaaagacag
     9361 aaacaaggtt acttggggat atgctaaaag cattttgaat acttggctta atgcaaactt
     9421 gaaatctatt gaacaagtac gtgcatttga aaagcaacaa cttgaaagca aaaaacaaaa
     9481 ttataaacct ttcgttaaac aatcaaaaga aaaaacaccc aaatggctca cagacagcac
     9541 gagagaaacg aaaacgccgg aagtagatga aaaccttgag aaagacagag aagcttttat
     9601 taagcgtcta aatagcaaat gggagtgatt gaaaatggat gcatttgata aatactatct
     9661 atttgatcat gacggcaaca aaatgttttc agttacacca cattttaaag atggacggca
     9721 tttagttgtt ggattaaaac atacaaaatt caatggtcga cgttggtact tagatgatta
     9781 tgaattaaaa acacttattg ataatgaaca aatggagtta ggacaccaaa caagcttatt
     9841 tgaatatata tgagggatta catggagata gaaattaaat ttaacgaaac tttcgaggca
     9901 cctatgggct cgcctcgacc gcgttttagc acaaaaggta gatatgcaca cacatatatg
     9961 cctacaaaat atacagaaca taaaaaatat ttacaaaatc aaatgccaaa gctaaatcta
    10021 gaaaatgcat taaaaattga attagagttt tactttactc cacctaaatc atggtcgaag
    10081 aaaaagaaaa ctcaagcgat tggacaatta aaagtaacta aaccagatat cgataatttg
    10141 atgaagacag ttctagacgc ttgtaacaat tatttgtgga aagatgacaa tcaaattgca
    10201 gaaataacta gctcaaagcg ttatggaatt gagcccaaaa taatcatacg aatagaagaa
    10261 atataagagg tggataaaat ggcgagaaaa gcaaggattg taacaataaa tgataaacca
    10321 tataggttta ccaaatctga aatggaatta atagaaagtc acggtataac cgctggaatg
    10381 gtttctaaga gagtaaaaga cggttgggaa ctacatgaag caatggacgc accagaaggt
    10441 acgcgtttaa gcgagtacag agaaaagaaa acaatagaaa gactggaaca agctagactc
    10501 gaacgcaaat tggaaagaaa gcgaaagaaa gaggctgagc taagaagaaa gaagccacat
    10561 ttgtttaatg tgcctcagaa acatccaaga ggacgttatg cgtgctacct gatggaaaac
    10621 gacatattcg tgaaagttaa gaagtagatc atgacagata acgcacgcaa agaataccta
    10681 aatcagttct ttggatctaa gagatatctg tatcaagata acgaacgagt ggcacatatc
    10741 catgtagcaa acggcaatta ttactttcac gggcatatcg taccaggttg gcaaggcgta
    10801 aagaagacgt ttgatacagc cgaagagcta gaaatatata taaagcaaca tggtttggaa
    10861 tacgagaaac agaagcaact aactttattt tagaggagat ggaaatgatg aataaccgcg
    10921 aacaaattga acagtccgtt ataagtgcta gtgcgtataa cggtaatgac acagagggat
    10981 tactaaaaga gattgaggac gtatataaga aagcgcaagc gtttgatgaa atacttaagg
    11041 gtttacctaa tgctatgcaa gatgcactca aagaagatat tggtcttgat gaggcagtag
    11101 ggattatgac gggtcaagtg gtctataaat atgaggaggc acaggaaaat gaataacaca
    11161 ttaacaattg atcaattaca agagttatta caaatacaaa aggagttcga cgatagaata
    11221 ccaacgctga acttacgaga tagcaaaata gcatatgtag ttgaattctt tgaatggttt
    11281 aatacattgg aaacgtttaa gaactggaag aagaaaccag gtaagccgtt agacgtacaa
    11341 cttgatgaat tagctgacat gttggcgttt ggattgagta ttgcgaatca agtaggagtg
    11401 tcatcagaag agataaaaga agcgattgaa tcaagtttta aagatacaga atttcacaaa
    11461 atgtttaatt ttaaagataa agaatttgct caagacgcag ttgttagtac accacagata
    11521 atattcaaag aattttatcc cgaccaacaa gcaattgtta tagtgataga catagcttac
    11581 aacttatatt ctatcgacca actcattgac gcatacaaaa agaaaatgaa aaggaaccac
    11641 gaaagacaag atggaacagc agacgcagga aaaggatacg tgtaaagaca tcttagatcg
    11701 agtcaaggag gttttgggga agtgagcgac atgttagaaa tatttttaat agggtttggc
    11761 gtttatctct tttatcgcat agcaattatt tttcttaaga gtaaaaagac tatacacaca
    11821 aacatatatg aaatgttaat gcttgctact atctttatga tatctacatt tgcttataaa
    11881 catcaaaaga cgcatatctt aatagcattt ttagtaatgt tttttatgag taagctcaaa
    11941 caagttcaag ggagctatga ggaatgacac aatacttagt cacaacattc aaagattcaa
    12001 caggacgtaa acatacacac ataactaaag ctaagagtaa tcaaaggttt acagttgttg
    12061 aggcagagag taaagaagaa gcgaaagaga agtacgagaa acaagttaaa agggatgcag
    12121 ttattaaagt gggtcagttg tttgaaaata taagggagtg tgggaaatga ttaaaaaact
    12181 taaaaatatg gatgggttcg acatctttat tgttggaata ctgtcattat tcggtataac
    12241 cgcattgcta cttgttgtcg cattgcctat ctatacagtg gctagttacc aaaacaaaga
    12301 agtacatcaa gggacaatta cagataaata taacaaaaga caagataaag aggacaaatt
    12361 ctatattgta ttagatgata aacaagtcat cgaaaactca gacttattct tcaaaggaaa
    12421 gtttgatagc gcagacatac aagctaggtt aaaagtaggt gataaagtaa aagttaagac
    12481 gattggatat agaatacact ttttaaattt atatccggtc ttatacgaag taaagaaggt
    12541 agataaaaaa tgattaagca aatactaaga ttattattct tactagcgat gtatgagtta
    12601 ggtaagtatg taactgagca agtatatatt atgatgacgg ctaatgatga tgtagaggcg
    12661 ccgagtgatt acgtctttcg agcggaggta agtgagtgat gtggattact atgactattg
    12721 tatttgctat attgctatta gtttgtatca gtattaatag tgatcgtgca agggagatac
    12781 aagcgctcag atatatgaat gattatctac ttgatgaagt agttaaaact aaaggataca
    12841 acgggttaaa agaatacagg attgaattaa agcgaatgaa taacgatatt aaaaagtaat
    12901 ttatattatc ggaggtattg cattgaatga taaagattga gaaacacgat atcaaaaagc
    12961 ttgaagaata cattcagcac atcgataact atcgaagaga gttgaagatg cgagaatatg
    13021 aattacttga aagtcatgaa ccagataatg cgggagctgg caaaagtaat ttgccgggta
    13081 acccgattga acgatgtgca ataaagaagt ttagtgataa caggtacaat acattaagaa
    13141 atatagttaa cggtgtagat agattgatag atgaaagtga tgaggatacg cttgagttat
    13201 taaggtttag atattgggat tgtcctattg gttgttatga atgggaagat atagcacatt
    13261 actttggtac aagtaagaca agtatattac gtagaaggaa tgcactgatc gataagttag
    13321 caaagtatat tggttatgtg tagcggactt ttaccctatg taagtccgca ttaaaacagt
    13381 ttattatgtt agtatcagat taatatttaa agttattaaa tgctaatacg acgcatgaac
    13441 aagaggcgca tcactatgtg atgtgtcttt ttatttatga ggtatgaaca tgttcaaact
    13501 aatagtaaat acattactac acatcaagta tagatgagtc ttgatactac ttaagttata
    13561 taaggtgaaa cattatgatg actaaagacg aacgcatacg attctataag tctaaagaat
    13621 ggcaaacaac aagaaaaaga gtgctagaaa gagataatta tgaatgtcaa caatgtaaga
    13681 gagacggcaa gttaacgaca tatgacaaaa gcaagcgtaa gtcgttggat gtagatcata
    13741 tattatcgct agaacatcat ccggagtttg ctcatgactt aaacaattta gaaacactgt
    13801 gtattaaatg tcacaacaaa aaagaaaaga gatttataaa aaaagaaaat aaatggaaag
    13861 acgaaaaatg gtaaataccc ccgggtcaaa aaaatcaaaa gcgatcaaaa tacttgggga
    13921 acgggcaggg gctcgacttc gcgataattt taaaaatcca tgtataaccc ccctcttata
    13981 accattttaa ggcaggtgat gaaatggaga ttatagttga tgaaaactta gtgcttaaag
    14041 aaaaagaaag gctgcaagta ttatataaag acatacctag caataaatta aaagtagttg
    14101 atggtttaat tattcaagca gcaaggctac gtgtaatgct tgattacatg tgggaagaca
    14161 taaaagaaaa aggtgactat gatttattta ctcaatctga aaaggcgcca ccatatgaaa
    14221 gggaaagacc agtagccaaa ctatttaatg ctagagatgc tgcatatcaa aaaataatca
    14281 aacaattatc ggatttattg cccgaagaga aagaagacac agaaacgcca tctgatgatt
    14341 acctatgatt agtaataaat acgttgatga atatataaat ttgtggaaac aaggaaagat
    14401 aattttaaat aaagaaagaa ttgatctctt taattatcta caaaaacata tatattcacg
    14461 agatgatgta tattttgatg aacagaaaat cgaggattgt atcaaattta ttgaaaaatg
    14521 gtattttcca acattaccat ttcaaaggtt tatcatagct aatatatttc ttatagataa
    14581 aaatacagat gaagctttct ttacagaatt tgctattttc atgggacgtg gaggcgggaa
    14641 aaacggtcta ataagtgcta ttagtgattt tctttctacg cccttacacg gagttaaaga
    14701 atatcacatc tccattgttg ctaatagtga agatcaagca aaaacatcgt ttgatgaaat
    14761 cagaaccgtt ttaatggata acaaacgaaa taagacgggt aaaacgccaa aagctcctta
    14821 tgaagttagt aaagcaaaaa taataaaccg tgcaactaaa tcggttattc gatataacac
    14881 atcaaacaca aaaaccaaag acggtggacg tgaggggtgt gttatttttg atgaaattca
    14941 ttatttcttt ggtcctgaaa tggtaaacgt caaacgtggt ggattaggta aaaagaaaaa
    15001 tagaagaacg ttttatataa gtactgatgg ttttgttaga gagggttata tcgatgcaat
    15061 gaagcacaaa attgcaagtg tattaagtgg caaggttaaa aatagtagat tgtttgcttt
    15121 ttattgtaag ttagacgatc caaaagaagt tgatgacaga cagacgtggg aaaaggcgaa
    15181 cccaatgtta cataaaccgt tatcagaata cgctaaaaca ctgctaagca cgattgaaga
    15241 agaatataac gatttaccat tcaaccgttc aaataagccc gaattcatga ctaagcgaat
    15301 gaatttgcct gaagttgacc ttgaaaaagt aatagcacca tggaaagaaa tactagcgac
    15361 taatagagag ataccaaatt tagataatca aatgtgtatt ggtggtttag actttgcaaa
    15421 cattcgagat tttgcaagtg tagggctatt attccgaaaa aacgatgatt acatttggtt
    15481 aggacattcg tttgtaagac aagggttttt ggatgatgtc aaattagaac ctcctattaa
    15541 agaatgggaa aaaatgggat tattgaccat tgtcgatgat gatgtcattg aaattgaata
    15601 tatagttgat tggtttttaa aggctagaga aaaatatggg cttgaaaaag tcatagctga
    15661 taattataga actgatattg taagacgtgc gtttgaggat gctggcataa aacttgaagt
    15721 acttagaaat ccaaaagcaa tacatggatt acttgcacca cgtatcgata caatgtttgc
    15781 gaaacataac gtaatatatg gagacaatcc tttgatgcgt tggtttacta ataatgttgc
    15841 agtaaaggtt aaacccgatg gtaataaaga atatattaaa aaagatgaaa atagaagaaa
    15901 aaccgatggg ttcatggctt ttgttcacgc attatataga gcagacgata tagtagacaa
    15961 agacatgtct aaagcgcttg atgcattaat gagtatagat ttctaataga ggaggtgaga
    16021 catgagtatt ctagaaaaga tatttaaaac taggaaagat ataacatata tgcttgattt
    16081 agatatgata gaagatctat cacaacaagc gtatgtgaaa cgtttagcga ttgatagttg
    16141 tattgaattt gttgcgcgag ctgtcgctca aagtcatttt aaagtattgg aaggtaatag
    16201 aattcaaaag aatgatgttt actacaagtt aaatataaaa ccaaatactg acttatcaag
    16261 cgatagtttt tggcaacaag ttatatataa actaatttat gataacgagg ttttaatcgt
    16321 agtaagtgac agcaaagaat tacttatcgc agatagcttt tacagagaag agtacgcttt
    16381 gtatgatgat atattcaaag atgtaacggt taaagattat acttatcaac gtactttcac
    16441 aatgcaagag gtcatatatt taaagtacaa caacaataaa gtgacacact ttgtagaaag
    16501 tctattcgaa gattacggga aaatattcgg aagaatgata ggtgcacaat taaaaaacta
    16561 tcaaataaga gggattttga aatctgcctc tagcgcatat gacgaaaaga atatagaaaa
    16621 attacaagcg ttcacaaata aattattcaa tacttttaat aaaaatcaac tagcaatcgc
    16681 gcctttgata gaaggttttg attatgagga attatctaat ggtggtaaga atagtaacat
    16741 gcctttttct gaattgagtg agctaatgag agatgcaata aaaaatgttg cgttgatgat
    16801 tggtatacct ccaggtttga tttacggaga aacagctgat ttggaaaaaa acacgcttgt
    16861 atttgagaag ttctgtttaa cacctttatt aaaaaagatt cagaacgaat taaacgcgaa
    16921 actcataaca caaagcatgt atttgaaaga tacaagaata gaaattgtcg gtgtgaataa
    16981 aaaagaccca cttcaatatg ctgaagcaat tgacaaactt gtaagttctg gttcatttac
    17041 aaggaatgag gtgcggatta tgttaggtga agaaccatca gacaatcctg aattagacga
    17101 atacctgatt actaaaaact acgaaaaagc taacagtggt gaaaatgatg aaaaagaaaa
    17161 agatgaaaac actttgaaag gtggtgatga agatgaaagc ggagattaaa ggcgtcatcg
    17221 tttccaacga agataaatgg gtttacgaaa tgcttggtat ggattcgact tgtcctaaag
    17281 atgttttaac acaactagaa tttagtgatg aagatgttga tattataatt aactcaaatg
    17341 gtggtaacct agtagctggt agtgaaatat atacacattt aagagctcat aaaggcaaag
    17401 tgaatgttcg tatcacagca atagcagcaa gtgcggcatc gcttatcgca atggctggtg
    17461 accacatcga aatgagtccg gttgctagaa tgatgattca caatccttca agtattgcgc
    17521 aaggagaagc gaaagatcta aatcatgctg cagaaacatt agaacatgtt ggtcaaataa
    17581 tggctgaggc atatgcggtt agagctggta aaaacaaaca agaacttgta gaaatgatgg
    17641 ctagggaaac gtggctaaat gctgatgaag ccattgaaca aggttttgcg gatagtaaaa
    17701 tgtttgaaaa cgacaatatg caaattgtag caaacaatac acaagtgtta tcgaaagatg
    17761 tattaaatcg tgtaacagct ttggtaagta aaacgccaga ggttaacatt gatattgacg
    17821 caatagcaaa taaagtaatt gaaaaaataa atatgaaaga aaaggaatca gaaatcgatg
    17881 ttgcagatag taaagtatca gcaaatggat tttcaagatt ccttttttaa tacaaaaaat
    17941 aggaggtcat aaaatgacta taaatttatc ggaaactttc gcaaatgcga aaaacgaatt
    18001 tattaatgca gtaaacaacg gtgaaccgca agaaagacaa aatgaattgt acggtgacat
    18061 gattaaccaa ctatttgaag aaactaaatt acaagcaaaa gcagaagctg aaagagtttc
    18121 tagtttacct aaatcagcac aatctttgag tgcaaaccaa agaagtttct tcatggatat
    18181 caataaaaac gttaactata aagaagaaaa acttttgcca gaagaaacaa ttgatagaat
    18241 ttttgaagat ttgacgacga atcatccgtt attagctgat ttaggtatta aaaacgctgg
    18301 tttgcgtttg aagttcttaa aatctgaaac ttctggcgta gccgtttggg gtaaaatcta
    18361 tggtgaaatt aaaggtcaat tagatgctgc gttcagtgaa gaaacagcaa ttcaaaataa
    18421 attgacagcg tttgttgttt taccaaaaga tttaaatgat tttggtcctg cgtggattga
    18481 aagatttgtt cgtgttcaaa tcgaagaagc atttgcagtg gcgcttgaaa ctgcgttctt
    18541 aaaaggtact ggtaaagacc aaccaatcgg cttaaaccgt caagtacaaa aaggtgtatc
    18601 ggtaactgag ggtgcttatc cagagaaaga agaacaaggt acgcttacat ttgctaatcc
    18661 gcgcgctacg gttaatgaat tgacgcaagt gtttaaatac cactcaacta acgagaaagg
    18721 taaatcagta gcggttaaag gtaatgtaac aatggttgtt aatccgtccg atgcttttga
    18781 ggttcaagca cagtatacac atttaaatgc aaatggcgta tatgttactg ctttaccatt
    18841 taatttgaat gttatcgagt ctacagtcca agaagcaggt aaggttttaa cgtacgttaa
    18901 aggtttatat gatggttatt tagctggtgg tattaatgtt cagaaattta aagaaacact
    18961 tgcgttagat gatatggatt tatacactgc aaaacaattt gcttacggca aagcgaaaga
    19021 taataaagtt gctgctgttt ggaaattaga tttaaaagga cataagccag ctttagaagg
    19081 taccgaagaa acactataaa attttatgag gtgataaaat ggtgaaattt aaagttgtta
    19141 gagcttttaa agacatagag cacaatcaac acaagtacaa agtaggggag ttgtatccag
    19201 ctgaagggta taacaatcct cgtgttgaat tgttgacaaa tcaaatcaaa aataagtacg
    19261 acaaagttta tatcgtacct ttagataagc tgacaaaaca agaattatta gaactatgcg
    19321 aatcattaca aaaaaaagcg tctagttcaa tggttaaaag tgaaatcgtc gacttattga
    19381 atggtgaaga caatgacgat tgatgatttg cttgtcaaat ttaaatcact tgaaaagatt
    19441 gaccataatt cagaggatga gtacttaaag cagttgttaa aaatgtcgta cgagcgtata
    19501 aaaaatcagt gcggagtttt tgaattagag aatttaatag gtcaagaatt gatacttata
    19561 cgcgctagat atgcttatca agatttatta gaacacttca acgataatta cagacctgaa
    19621 ataatagatt tttcgttatc tctaatggag gtatcagaag atgaagaaag tgtttaaaaa
    19681 acctagaatt acaactaaac gtttaaatac tcgtgttcat ttttataagt atactgaaaa
    19741 taatggtcca gaagctggag aaaaagaaga aaaattatta tatagctgtt gggcgagtat
    19801 tgatggtgtc tggttacgtg aattagaaca agctatctca aacggaaccc aaaatgacat
    19861 taaattgtat attcgtgatc cgcaaggtga ttatttaccc agtgaagaac attatcttga
    19921 aattgaatca agatatttca aaaatcgttt gaatataaag caagtatcac cagatttgga
    19981 taataaagac tttattatga ttcgtggagg atatagttca tgagtgtgaa agtgataggt
    20041 gataaagcat tagaaagaga attagaaaaa cgttttggca taaaagagat ggtaaaagtt
    20101 caagataagg cgttaatagc tggtgctaag gtaattgttg aagaagtaaa aaaacaacta
    20161 aagccctcaa aagatacggg agcattaatt aatgaggtaa gttttagtaa acctgaatgg
    20221 ataaacggaa aacgtacaat tactgttcat tggcgaggtt ctaaagaccg ttataaaatc
    20281 gtacatttaa ttgaatatgg acacgttcaa aaaggaacag gtaaatttat caaacctaaa
    20341 gctatgggcg gtgttaatag agcaataaga caagggcaaa ataagtattt tgagacgcta
    20401 aaaagggagt tgaaaaaatt gtgattgata ttttgtacaa agttcatgaa gtgattagtc
    20461 aagacagaat tattagagag cacgtaaata tcaataatat taagttcaat aaatacccta
    20521 atgtaaaaga tactgatgta ccttttattg ttattgacga tatcgacgac ccaataccta
    20581 caacttatac tgacggagat gagtgtgcat atagttatat tgtccaaata gatgtttttg
    20641 ttaagtacaa tgatgaatat aatgcgagaa tcataagaaa taagatatct aatcgcattc
    20701 aaaagttatt atggtctgaa ctaaaaatgg gaaatgtttc aaatggaaaa ccggaatata
    20761 tagaagaatt taaaacatat agaagctctc gcgtttacga gggcattttt tataaggagg
    20821 aaaattaaat ggcagtaaaa catgcaagtg cgccaaaggc gtatattaac attactggtt
    20881 taggtttcgc taaattaacg aaagaaggcg cggaattaaa atatagtgat attacaaaaa
    20941 caagaggatt acaaaaaatt ggtgttgaaa ctggaggaga cttgaaaaca gcatatgctg
    21001 atggtggtcc aatcgaatca gggaacacag acggagaagg taaaatttcg ttacaaatgc
    21061 atgctttccc taaagagatt cgcaaaatag tattcaatga agagtatgat gaagacggtg
    21121 tttataaaga aactcaaggt aaacaaaaca actatgtagc aatttggttc agacaagagc
    21181 gtcgagacgg cacatttaga acggttttat tacctaaagt catgtttaca aatcctaaaa
    21241 tcgatggaga aacggctgag aaagattggg atttctcaag tgaagaggtt gaaggtgagg
    21301 cacttttccc tttagttgat aataaaaagt cagtacgtaa gtatatcttt gattcagcta
    21361 acatgacaaa tcatgatgga gacggtgaaa aaggcgaaga ggctttctta aagaaaattt
    21421 taggcgaaga atatactgga aacgtgacag agggtaacga agaaactttg taacaaaacc
    21481 ggcttcatcg gaaactgcgg taaagtcggt taatatacca gatagcatta aaacacttaa
    21541 agttggcgac acatacgatt taaatgttgt agtagagcca tctaatcaaa gtaagttatt
    21601 gaaatacaca acagatcaaa cgaatattgt atcaatcaat agtgatggtc aagttactgc
    21661 ggaagcacaa ggcattgcta cggttaaagc aacagttggt aatatgagtg acactataac
    21721 aataaatgta gaagcataag agggggcaac ccctctattt tatttgaaaa taaggagagt
    21781 attataaaat ggcaaaatta aaacgtaaca ttattcaatt agtagaagac ccgaaagcaa
    21841 atgaaattaa attacaaacg tacttaacac cacacttcat ttcatttgaa attgtatacg
    21901 aagcaatgga tttaatcgat gatattgagg acgaaaatag cacgatgaaa ccaagagaaa
    21961 tcgctgacag attgatggat atggttgtaa aaatttacga taaccaattc acagttaaag
    22021 acctaaaaga acgtatgcat gcacctgatg gaatgaatgc acttcgtgaa caagtgattt
    22081 tcattactca aggtcagcaa actgaggaaa ctagaaattt tatccagaac atgaaataaa
    22141 gcctgaagat ttaacatata aagcaatgtt gaaaaatatg gatactctca tgatggactt
    22201 aattgaaaat ggtaaagacg ctaacgaagt tttaaaaatg ccatttcatt atgtactttc
    22261 catatatcaa aataaaaaca atgacatttc tgaagaaaaa gcagaggctt taattgatgc
    22321 gttttaacct taaccgtttg gttagggtta tttttttgaa cttttttaga aaggaggtaa
    22381 aaaatgggag aaagaataaa aggtttatct ataggtttgg atttagatgc agcaaattta
    22441 aatagatcat ttgcagaaat caaacgaaac tttaaaactt taaattctga cttaaaatta
    22501 accggtaaca acttcaaata taccgaaaaa tcaactcata gttacaaaca aaggattaaa
    22561 gaacttgatg gaactatcac aggttataag aaaaacgttg atgatttagc caagcaatat
    22621 ggcaaggtat ctcaagaaca gggcgaaaac agcgcggaag ctcaaaaatt acgacaagaa
    22681 tataacaaac aagcaaatga gctgaatttt ttagaaaaag aactagaaaa aacaacaact
    22741 gagtttgaag agttcaaaaa agctcaagtt gaagctcaaa gaatggcaga aagtggctgg
    22801 ggaaaaacca gtaaagtttt tgaaagtatg ggacctaaat taacaaaaat gggtgatggt
    22861 ttaaaatcca ttggtaaagg tttgatgatt ggtgtaactg cacctgtttt aggtattgca
    22921 gcagcatcag gaaaagcttt tgcagaagtt gataaaggtt tagatacagt tacccaagca
    22981 acaggagcaa ccggcggaga gcttaagaag ttgcagaatt catttaaaga tgtttatggc
    23041 aactttccag cagacgctga gactgtaggc ggtgttttag gggaagttaa cacaaggtta
    23101 ggtttcactg gcaaagaact tgagagtgcc acagagtcat tcttgaaatt tagtcacata
    23161 acaggttctg acggcgtaca agccgttcaa ttaattacgc gtgcaatggg tgatgcaggt
    23221 attgaagctg atgagtatca aagtgtactt gatatggtag cgaaagcagc acaggctagc
    23281 ggtataagtg ttgatacatt agctgatagc attactaaat acggtgctcc aatgagggct
    23341 atgggctttg agatgaaaga atcaatcgct ttattctctc aatgggagaa atcaggtgtt
    23401 aatactgaaa tagccttcag tggtttgaaa aaagctatat ccaattgggg taaagcgggt
    23461 aaagacccaa gagaagaatt taagaagaca ttagcagaaa ttgaaaggac accggatata
    23521 gctagcgcaa caagtttagc gattgaagca tttggtgcaa aagcaggtcc tgatttagca
    23581 gatgctatta aaggcggtcg ctttagttac caagagttct taaaaactat cgaagattcg
    23641 caaggaacgg tcaatcagac atttaaagat tctgaaagtg gctccgaaag atttaaagta
    23701 gcaatgaata aacttaaatt agtaggtgct gatgtatggg cttctattga aagtgcgttt
    23761 gctccagtca tggaagaatt aatcaaaaag ctatctgtag cagttgattg gttttcaagt
    23821 ttaagtgatg gatctaaaag gtcgattgtt atattcggtg gtattgctgc tgcaattggt
    23881 cctgtagttt ttggattagg tgcattcata agcacaattg gcaacgcagt aactgtatta
    23941 gctccattat tagctagtat tgtaaaggct ggcggattga ttagtttttt atcaactaaa
    24001 gtgcctattt taggaacagt cttcacagca ttaactggtc caattggtat cgtgttaggt
    24061 gtactggctg gtttagcagt cgcatttaca atagcttata agaaatctga aacattcaga
    24121 aattttgtta atggtgcaat taacagtgtt aaacaaacgt ttagtaattt cattcaattt
    24181 atccaacctt tcattgattc cgttaaaaac gtctttaaac aagcggtttc agcaatcgtt
    24241 gatttcgcta aagatatttg gagtcaaatt aatggattct ttaatgaaaa cggaatttct
    24301 attgttcaag cgcttcaaaa tatatgcaat tttatcaaag ctatatttga atttatctta
    24361 aattttgtaa ttaaaccaat catgtttgcg atttggcaag tgatgcaatt tatttggccg
    24421 gcggttaaag ccttgattgt cagtacttgg gagaatataa aaggagtaat acaaggtgct
    24481 ttaaatatca tacttggctt tattaagttc ttttcaagtt tattcactgg taattggcga
    24541 ggtgtttggg acggtattgt gatgatacta aaaggcactg tgcagttaat ttggaattta
    24601 atacaactgt ggtttgtagg taagattcta ggtgttgtta gatactttgg tggattgctt
    24661 aaaggtttaa tatccggtat ctggggtgtt atcaaaggta ttttcacaaa atcattatct
    24721 gcaatttgga atgcaacgaa aagtattttt ggtttcttat acaatagtgt taaatctatt
    24781 ttcactaata tgaaaaactg gttatctagt acgtggaata atatcaaaag caataccgtc
    24841 ggcaaggctc attcgttatt tacgggtgta aggtctaaat tcacaagttt atggaatgcg
    24901 acgaaagata tatttactaa attaagaaat tggatgtcaa acatctggaa ctctattaaa
    24961 gataacacgg taggtatagc gggtcgctta tgggatagag tgcgtaacat ctttggaagc
    25021 atgcgtgacg gtttaaaatc tatcattagt aaaattaaag atcatatcgg tggtatggta
    25081 gacgctgtta aaagaggtct taataaatta attgaaggtt taaactgggt cggtggtaag
    25141 ttgggtatgg acaaaatacc gaagttacac actggtactg aacatacaca tactactaca
    25201 agattagtta agaacggtaa gattgcacgt gacacattcg ctacagttgg ggataaggga
    25261 cgcggaaatg gtccaaatgg tttcagaaat gaaatgattg aattccctaa tggtaaacgt
    25321 gtaatcacac caaatacaga tactacggct tatttaccta aaggctcaaa agtatacaac
    25381 ggggcacaaa cttattcaat gttaaacgga acgcttccga gatttcattt cggtactact
    25441 atgtggaaag atattaaatc tagtgcatca tcggcattta actggacaaa agatcaaata
    25501 ggtaaaggta ccaaatggct tggcgataaa gttggcgatg taatggactt tattgataat
    25561 ccgggtaagc ttttaaatta tgtgctcaaa gcgtttggtg ttgactttag ctctctaact
    25621 aaaggtatgg gtattgttgg cgatataaca aaagcgtctt ggaataagat taaaagtaag
    25681 gcgattaatt ggataaaaga aggattagag agccaagcgg gagatggttc tgtgtttgat
    25741 agtttcagaa tactacaacc ttattcagca ccgccaaaac ctcctaaccc caattatcca
    25801 tttaacggag gcgttcatca tggtgttgac tatgatacgc cgaccggtac ccctatacgt
    25861 acgccaatgg gtggacgtgt tagaagttgg tatgacaact atggtggcgg taaagcaatt
    25921 actgttcaaa aaggtcgaac atttttgtgg ttcatgcact tatctgaaca attgcgtaga
    25981 acaggtgaac aaattaaagc tggtcaatta attggtaaat caggtaatac aggttctatg
    26041 actaattacc gccatttaca tttccaagtc aatcaaggcg gagagtccaa taggtattcg
    26101 acagacccta ttccttggtt acggaaaaac gacaaaactg gtggaaagaa ttcacctgga
    26161 gggagtggtt ctgaaaatgc gcgcagagcg attagaacag ctcaaaatat acttggaggt
    26221 caatacaaag ctagctggat tacacacgaa atgatgcgtg tagcaagacg tgaatccaat
    26281 tatacagcta atgcagttaa taattgggat agcaacgcaa gagctggtac accttcaaga
    26341 ggtatgttcc aaatgataga tccttcattt agagcgtacg caaagtcggg ttacaataat
    26401 cctcttaacc caactcatca agctatatcg gctatgagat atattgtggg taaatgggta
    26461 ccaagaacgg gctcatggag agctgcgttc aaacgcgctg gtgattacgc atatgctact
    26521 ggtggcaaag tctacaacgg attgtaccac ttaggagaag aaggatatcc agagtgggtt
    26581 attcctactg atcctgcacg taaaaacgaa gcaatgaaga tgttacatta tgcagcagca
    26641 gaagtaagag ggagaaaagc gagtaaaaat aagcgtccta gtcaattgtc tagtgtaaat
    26701 gggtttgatg acccaagctt attattgaaa atgattgaac aacagcaaca acaaatagct
    26761 ttattactga aaatagcgca atccaacgat gtgattgcag ataaagatta tcagccgatt
    26821 attgacgaat acgcttttga taaaaaggtg aacgcgtcta tagaaaagcg agaaaggcaa
    26881 gaatcaacaa aagtaaagtt tagaaaagga ggaattgcta ttcaatgata gacactatta
    26941 aagtgaacaa caaaacaatt ccttggttgt atgtcgaaag agggtttgaa ataccctctt
    27001 ttaattatgt tttaaaaact gaaaatgtag atggacgttc ggggtctata tataaagggc
    27061 gtaggcttga atcttatagt tttgatatac ctttggtggt acgtaatgac tatttatctc
    27121 acaacggctt taaaacacat gatgacgtct tgaatgaatt agtaaagttt tttaactacg
    27181 aggaacaagt taaattacaa ttcaaatcta aagattggta ctggaacgct tattttgaag
    27241 gaccaataaa gctgcacaaa gaatttgcaa tacctgttaa gttcactatc aaagtagtac
    27301 taacagaccc ttacaaatat tcagtaacag gatataaaaa tactgcgatt tcagaccaag
    27361 tttcagttgt aaatagtggg actgctgaca ctcctttaat tgttgaagcc cgagcaatta
    27421 aaccatctag ttactttatg atcactaaaa atgatgaaga ttattttatg gttggtgatg
    27481 atgaggtaac caaagaagtt aaggattaca tgcctcctgt ttatcatagt gagtttcgtg
    27541 atttcaaagg ttggactaag atgattactg aagatattcc aagtaatgat ttaggtggta
    27601 aagtcggcgg tgactttgtg atatccaatc ttggcgaagg atataaagca actaattttc
    27661 ctgatgcaaa aggttgggtt ggtgctggca cgaaacgagg gctccctaaa gcgatgacag
    27721 attttcaaat tacctataaa tgtattgttg aacaaaaagg taaaggtgcc ggaagaacag
    27781 cacaacatat ttatgatagt gatggtaagt tacttgcttc tattggttat gaaaataaat
    27841 atcatgatag aaaaatagga catattgttg ttacgttgta taaccaaaaa ggagacccca
    27901 aaaagatata cgactatcag aataaaccga taatgtataa cttggacaga atcgttgttt
    27961 atatgcggct cagaagagta ggtaataaat tttctattaa aacttggaaa tttgatcaca
    28021 ttaaagaccc agatagacgt aaacctattg atatggatga gaaagagtgg atagatggcg
    28081 gtaagtttta tcagcgtcca gcttctatca tagctatcta tagtgcgaag tataatggtt
    28141 ataagtggat ggagatgaat ggattaggtt cattcaatac ggagattcta ccaaaaccga
    28201 aaggcgcaag ggatgtcatt atacaaaaag gtgatttagt gaaaatagat atgcaagcaa
    28261 aaagtgttgt catcaatgag gaaccaatgt tgagcgagaa atcgtttgga agtaattatt
    28321 tcaatgttga ttctgggtac agtgaattaa tcatacaacc tgaaaacgtc tttgatacga
    28381 cggttaaatg gcaagataga tatttataga aaggagatga gagtgtgata catgttttag
    28441 attttaacga caagattata gatttccttt ctactgatga cccttcctta gttagagcga
    28501 ttcataaacg taatgttaat gacaattcag aaatgcttga actgctcata tcatcagaaa
    28561 gagctgaaaa gttccgtgaa cgacatcgtg ttattataag ggattcaaac aaacaatggc
    28621 gtgaatttat tattaactgg gttcaagata cgatggacgg ctacacagag atagaatgta
    28681 tagcgtctta tcttgctgat ataacaacag ctaaaccgta tgcaccaggc aaatttgaga
    28741 aaaagacaac ttcagaagca ttgaaagatg tgttgagcga tacaggttgg gaagtttctg
    28801 aacaaaccga atacgatggc ttacgtacta cgtcatggac ttcttatcaa actagatatg
    28861 aagttttaaa gcaattatgt acaacctata aaatggtatt ggatttttat atagagctta
    28921 gttctaatac cgtcaaaggt agatatgtgg tactcaaaaa gaaaaacagc ttattcaaag
    28981 gtaaagaaat tgagtatggt aaagatttgg ttgggttaac taggaagatt gatatgtcag
    29041 aaatcaaaac agcattaatt gctgtgggac ccgaaaatga caaaggaaag cgtttagagt
    29101 tagttgtgac tgatgacgaa gcacaaagtc aattcaactt acctacccgt tatatttggg
    29161 gaatatacga acctcaatca gatgatcaaa atatgaatga aacacggttg cgttctttag
    29221 ccaaaacaga gttaaataaa cgtaagtcgg cagttatgtc atatgagatt acttctactg
    29281 atttggaagt tacgtatccg cacgagatta tatcaattgg tgatacagtc agagtaaaac
    29341 atagagattt taacccgcca ttgtatgtag aggcagaagt tattgccgaa gaatataaca
    29401 taatttcaga aaatagcaca tatacattcg gtcaacctaa agagttcaaa gaatcagaat
    29461 tacgagaaga gtttaacaag cgattgaaca taatacatca aaagttaaac gataatatta
    29521 gcaatatcaa cactatagtt aaagatgttg tagatggtga attagaatac tttgaacaca
    29581 aaatacacaa aagtgataca ccgccagaaa atccagtcaa tgatatgctt tggtatgata
    29641 caagtaaccc tgatgttgct gtcttgcgta gatattggaa tggtcgatgg attgaagcaa
    29701 caccaaatga tgttgaaaaa ttaggtggta taacaagaga gaaagcgcta ttcagtgaat
    29761 taaacaatat ttttattaat ttatctatac aacacgctag tcttttgtca gaagctacag
    29821 aattactgaa tagcgagtac ttagtagata atgatttgaa agaggactta caagcaagtt
    29881 tagacgctgt gattgatgtt tataatcaaa ttaaaaataa tttagaatct atgacacccg
    29941 aaactgcaac gattggtcgg ttggtagata cacaagcttt atttcttgag tatagaaaga
    30001 aattacaaga tgtttataca gatgtagaag atgtcaaaat ggccatttca gatagattta
    30061 aattattaca gtcacaatac actgatgaaa aatataaaga agcgttggaa ataatagcaa
    30121 caaaatttgg tttaacggtg aatgaagatt tgcagttagt cggagaacct aatgttgtta
    30181 aatcagctat tgaagcagct agagaatcca caaaagaaca attacgtgac tatgtaaaaa
    30241 catcggacta taaaatagac aaagacggta ttgttgaacg tttagatact gctgaagctg
    30301 agagaacgac tttaaaaggt gaaatcaaag ataaagttac gttaaacgaa tatcgaaacg
    30361 gattggaaga acaaaaacaa tatactgatg accagttaag tgatttgtcc aataatcctg
    30421 agattaaagc aagtattgaa caagcaaatc aagaagcgca agaagcttta aaatcataca
    30481 ttgatgctca agatgatctt aaagagaagg aatcgcaagc gtatgctgat ggtaaaattt
    30541 cggaagaaga gcaacgcgct atacaagatg ctcaagctaa acttgaagag gcaaaacaaa
    30601 acgcagaact aaaggctaga aacgctgaaa agaaagctaa tgcttataca gacaacaagg
    30661 tcaaagaaag cacagatgca cagaggaaaa cactgactcg ctatggttct caaattatac
    30721 aaaatggtaa ggaaatcaaa ttaagaacta ctaaagaaga gtttaatgca accaatcgta
    30781 cactttcaaa tatattaaac gagattgttc aaaatgttac agatggaaca acaatcagat
    30841 atgatgataa cggagtggct caagctttga atgtggggcc acgtggtatt agattaaatg
    30901 ctgataaaat tgatattaac ggtaatagag aaataaacct tcttatccaa aatatgcgag
    30961 ataaagtaga taaaaccgat attgtcaaca gccttaattt atcaagagag ggtcttgata
    31021 tcaatgttaa tagaattgga attaaaggcg gtgacaataa cagatatgtt caaatacaga
    31081 atgattctat tgaactaggt ggtattgtgc aacgtacttg gagagggaaa cgttcaacag
    31141 acgatatttt tacgcgactg aaagacggtc acctgagatt tagaaataac accgctggcg
    31201 gttcacttta tatgtcacat tttggtattt cgacttatat tgatggtgaa ggtgaagacg
    31261 gtggttcatc tggtacgatt caatggtggg ataaaactta cagtgatagt ggcatgaatg
    31321 gtataacaat caattcctat ggtggtgtcg ttgcactaac gtcagataat aatcgggttg
    31381 ttctggagtc ttacgcttca tcgaatatca aaagcaaaca ggcaccggtg tatttatatc
    31441 caaacacaga caaagtgcct ggattaaacc gatttgcatt cacgctgtct aatgcagata
    31501 atgcttattc gagtgacggt tatattatgt ttggttctga tgagaactat gattacggtg
    31561 cgggtatcag gttttctaaa gaaagaaata aaggtcttgt tcaaattgtt aatggacgat
    31621 atgcaacagg tggagataca acaatcgaag cagggtatgg caaatttaat atgctgaaac
    31681 gacgtgatgg taataggtat attcatatac agagtacaga cctactgtct gtaggttcag
    31741 atgatgcagg agataggata gcttctaact caatttatag acgtacttat tcggccacag
    31801 ctaatttgca tattacttct gctggcacaa ttgggcgttc gacatcagcg cgtaaataca
    31861 agttatctat cgaaaatcaa tataacgata gagatgaaca actggaacat tcaaaagcta
    31921 ttcttaactt acctattaga acgtggtttg ataaagctga gtctgaaatt ttagctagag
    31981 agctgagaga agatagaaaa ttatcggaag acacctataa acttgataga tacgtaggtt
    32041 tgattgctga agaggtggag aatttaggat taaaagagtt tgtcacgtat gatgacaaag
    32101 gagaaattga aggtatagcg tatgatcgtc tatggattca tcttatccct gttatcaaag
    32161 aacaacaact aagaatcaag aaattggagg agtcaaagaa tgaaggataa caaacaagga
    32221 ttacaagcta atcctgaata tacaattcat tatttatcac aggaaattat gaggttaaca
    32281 caagaaaacg cgatgttaaa agcgtatata caagaaaata aagaaaatca acaatgtgct
    32341 gaggaagagt aatccttagc actattttta tacaaaaatt taaggaggtc atttaattat
    32401 ggcaaaagaa attatcaaca atacagaaag gtttatttta gtacaaatcg acaaagaagg
    32461 tacagaacgt gtagtatatc aagatttcac aggaagtttt acaacgtccg attcagcaag
    32521 ttatgcacaa gattttaaat ctgaggaaaa cgctaaaaag attgctgaaa ctttaaatct
    32581 tttatatcaa ttaacaggca atcaaaacgg tgtgaaagtt gtgaaagaag ttgtggatag
    32641 aactgacttg tcatctgata aatcagttga tagcgaaaca atgtaactat actaagttat
    32701 gagcattacg ctcatagctt tcttagaaag taggtgtagt tttggatgat attcagaaaa
    32761 taaaaaaaga gctttctgaa ttagttgaac gtgttgatga tgttgaaata ctagcaaatg
    32821 aaacagctga tcatgtgctt gaacttagag aggaacataa gcaacatcat aatgaactaa
    32881 gagaatctca taaagaactt aaagataagc aagataaagt tgtagatgag aatttagagc
    32941 aaacaaagat attaaacaga attgaagaaa gatatcaaac gcaagtagat gttgcgcaaa
    33001 aaaatgaaga aaagacactc gcccaaaata aatggctcgt aggtgccata tgggcgcttg
    33061 taacaattgt tatgattgca gtcattactg catcaattac tgcgttatta ccttaaggga
    33121 ggtggacata atgagttggg caagatggtt atcatgttat ttgtatggtc gtaaatgtaa
    33181 ataatgtttt tggtcagtgc atcggcactg gctttttatt ttgattgaaa agaggtacgt
    33241 acatggtatt acacagctca aaagacagga agcatactgc aagtgaagtt gggaagtgtt
    33301 gtcaatacca agtaagtaag atatctgaaa tgtataatag agtaaaaatg aaatcttttt
    33361 attatattat agacaagtat aaaaaaggta ta
//
LOCUS       DAXWFK010000008        32311 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.008, whole genome shotgun sequence.
ACCESSION   DAXWFK010000008 DAXWFK010000000
VERSION     DAXWFK010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 32311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32311
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.008"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(94..1731)
                     /locus_tag="ACGEXS_000314"
     CDS             complement(94..1731)
                     /locus_tag="ACGEXS_000314"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFF8011362.1"
                     /translation="MSYSIGIDYGTASGRVFLINTTNGQVVSKFVKPYTHGVIESELN
                     GLKIPHTYALQNSNDYLEIMEEGISYIVRESKIDPDNIVGIGIDFTSSTIIFTDENLN
                     PVHNLKQFKNNPHAYVKLWKHHGAYKEAEKLYQTAIENNNKWLGHYGYNVSSEWMIPK
                     IMEVMNRAPEIMEKTAYIMEAGDWIVNKLTNKNVRSNCGLGFKAFWEEETGFHYDLFD
                     KIDPKLSKVIQDKVSAPVVNIGEAVGKLDDKMAQKLGLSKETMVSPFIIDAHASLLGI
                     GSEKDKEMTMVMGTSTCHLMLNEKQHQVPGISGSVKGAIIPELFAYEAGQSAVGDLFE
                     YVAKQAPKSYVDEAENRNMTVFELMNEKIKHQMPGESGLIALDWHNGNRSVLSDSNLT
                     GCIFGLTLQTKHEDIYRAYLEATAFGTKMIMQQYQDWHMEVEKVFACGGIPKKNAVMM
                     DIYANVLNKKLIVMDSEYAPAIGAAILGAVSGGAHNSINDAVDAMKEPILYEINPEAE
                     KVQRYETLFKAYKALHDIHGYKKANIMKDIQSLRVEG"
     gene            complement(1891..2769)
                     /gene="hchA"
                     /locus_tag="ACGEXS_000315"
     CDS             complement(1891..2769)
                     /gene="hchA"
                     /locus_tag="ACGEXS_000315"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFF8011363.1"
                     /translation="MSQDVNELSKQPTPDKAEDNAFFPSPYSLSQYTAPKTDFDGVEH
                     KGAYKDGKWKVLMIAAEERYVLLENGKMFSTGNHPVEMLLPLHHLMEAGFDVDVATLS
                     GYPVKLELWAMPTEDEAVISTYNKLKEKLKQPKKLADVIKNELGPDSDYLSVFIPGGH
                     AAVVGISESEDVQQTLDWALDNDRFIVTLCHGPAALLSAGLNREKSPLEGYSVCVFPD
                     SLDEGANIEIGYLPGRLKWLVADLLTKQGLKVVNDDMTGRTLKDRKLLTGDSPLASNE
                     LGKLAVNEMLNAIQNK"
     gene            complement(3035..4222)
                     /locus_tag="ACGEXS_000316"
     CDS             complement(3035..4222)
                     /locus_tag="ACGEXS_000316"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFF8011364.1"
                     /translation="MVQSLHEFLEENINYLKENGLYNEIDTIEGANGPEIKINGKSYI
                     NLSSNNYLGLATNEDLKSAAKAAIDTHGVGAGAVRTINGTLDLHDELEETLAKFKGTE
                     AAIAYQSGFNCNMAAISAVMNKNDAILSDELNHASIIDGCRLSKAKIIRVNHSDMDDL
                     RAKAKEAVESGQYNKVMYITDGVFSMDGDVAKLPEIVEIAEEFGLLTYVDDAHGSGVM
                     GKGAGTVKHFGLQDKIDFQIGTLSKAIGVVGGYVAGTKELIDWLKAQSRPFLFSTSLA
                     PGDTKAITEAVKKLMDSTELHDKLWNNAQYLKNGLSKLGYDTGESETPITPVIIGDEK
                     TTQEFSKRLKDEGVYVKSIVFPTVPRGTGRVRNMPTAAHTKDMLDEAIAAYEKVGKEM
                     KLI"
     gene            4393..5568
                     /locus_tag="ACGEXS_000317"
     CDS             4393..5568
                     /locus_tag="ACGEXS_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFF8011365.1"
                     /translation="MLDWFQLANNKENKTIQLRRYLHQYPELSFEEFQTHDYIVNQLS
                     QLSCDIETPIGRNGIKATFKGLGTGPTIALRADFDALPVEELNDVPYKSKNPGCMHAC
                     GHDGHTAILLTVAEILDEHKHLLEGNVVLIFQYGEEIMPGGSQEMIDAGCLENVDRIY
                     GTHLWSGYPTGTIHSRAGAIMASPDEFSVTFKGRGGHGAKPHETIDPIVIMAEFILSA
                     QKIISRTIDPVKQAVLSFGMIQAGTTDSVIPDQAFCKGTVRTFDSDIQNHVMDKMDKL
                     LQGLAIANDINYDLNYIKGYLPVHNNEKAYQVIKEATNDLHVRFNESDLMMIGEDFSH
                     YLKVRPGAFFLTGCGNESKGITAPHHNPKFDIDEKSLKYAVAVFLKIIELEQVFKTN"
     gene            complement(5850..7034)
                     /gene="tuf"
                     /locus_tag="ACGEXS_000318"
     CDS             complement(5850..7034)
                     /gene="tuf"
                     /locus_tag="ACGEXS_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFF8011366.1"
                     /translation="MAKEKFDRSKEHANIGTIGHVDHGKTTLTAAIATVLAKNGDSVA
                     QSYDMIDNAPEEKERGITINTSHIEYQTDKRHYAHVDCPGHADYVKNMITGAAQMDGG
                     ILVVSAADGPMPQTREHILLSRNVGVPALVVFLNKVDMVDDEELLELVEMEVRDLLSE
                     YDFPGDDVPVIAGSALKALEGDAQYEEKILELMEAVDTYIPTPERDSDKPFMMPVEDV
                     FSITGRGTVATGRVERGQIKVGEEVEIIGLHDTSKTTVTGVEMFRKLLDYAEAGDNIG
                     ALLRGVAREDVQRGQVLAAPGSITPHTEFKAEVYVLSKDEGGRHTPFFSNYRPQFYFR
                     TTDVTGVVHLPEGTEMVMPGDNVEMTVELIAPIAIEDGTRFSIREGGRTVGSGVVTEI
                     IK"
     gene            complement(7251..9332)
                     /gene="fusA"
                     /locus_tag="ACGEXS_000319"
     CDS             complement(7251..9332)
                     /gene="fusA"
                     /locus_tag="ACGEXS_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFF8011367.1"
                     /translation="MAREFSLEKTRNIGIMAHIDAGKTTTTERILYYTGRIHKIGETH
                     EGASQMDWMEQEQDRGITITSAATTAAWEGHRVNIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFVNKMDKLGANFEYSVSTLHDRLQANAA
                     PIQLPIGAEDEFEAIIDLVEMKCFKYTNDLGTEIEEIEIPEDHLDRAEEARASLIEAV
                     AETSDELMEKYLGDEEISVSELKEAIRQATTNVEFYPVLCGTAFKNKGVQLMLDAVID
                     YLPSPLDVKPIIGHRASNPEEEVIAKADDSAEFAALAFKVMTDPYVGKLTFFRVYSGT
                     MTSGSYVKNSTKGKRERVGRLLQMHANSRQEIDTVYSGDIAAAVGLKDTGTGDTLCGE
                     KNDIILESMEFPEPVIHLSVEPKSKADQDKMTQALVKLQEEDPTFHAHTDEETGQVII
                     GGMGELHLDILVDRMKKEFNVECNVGAPMVSYRETFKSSAQVQGKFSRQSGGRGQYGD
                     VHIEFTPNETGAGFEFENAIVGGVVPREYIPSVEAGLKDAMENGVLAGYPLIDVKAKL
                     YDGSYHDVDSSEMAFKIAASLALKEAAKKCDPVILEPMMKVTIEMPEEYMGDIMGDVT
                     SRRGRVDGMEPRGNAQVVNAYVPLSEMFGYATSLRSNTQGRGTYTMYFDHYAEVPKSI
                     AEDIIKKNKGE"
     gene            complement(9455..9925)
                     /gene="rpsG"
                     /locus_tag="ACGEXS_000320"
     CDS             complement(9455..9925)
                     /gene="rpsG"
                     /locus_tag="ACGEXS_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFF8011368.1"
                     /translation="MPRKGSVPKRDVLPDPIHNSKLVTKLINKIMLDGKRGTAQRILY
                     SAFDLVEQRSGRDALEVFEEAINNIMPVLEVKARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVNYARLRGEKTMEDRLANEILDAANNTGGAVKKREDTHKMAEANKAFAHYRW"
     gene            complement(9991..10404)
                     /gene="rpsL"
                     /locus_tag="ACGEXS_000321"
     CDS             complement(9991..10404)
                     /gene="rpsL"
                     /locus_tag="ACGEXS_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFF8011369.1"
                     /translation="MPTINQLVRKPRQSKIKKSDSPALNKGFNSKKKKFTDLNSPQKR
                     GVCTRVGTMTPKKPNSALRKYARVRLSNNIEINAYIPGIGHNLQEHSVVLVRGGRVKD
                     LPGVRYHIVRGALDTSGVDGRRQGRSLYGTKKPKN"
     gene            complement(10502..10756)
                     /locus_tag="ACGEXS_000322"
     CDS             complement(10502..10756)
                     /locus_tag="ACGEXS_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HFF8011370.1"
                     /translation="MSKEKVARFNKQHFVVGLKETLKALKKDQVTSLIIAEDVEVYLM
                     TRVLSQINQKNIPVSFFKSKHALGKHVGINVNATIVALIK"
     gene            complement(10893..14489)
                     /gene="rpoC"
                     /locus_tag="ACGEXS_000323"
     CDS             complement(10893..14489)
                     /gene="rpoC"
                     /locus_tag="ACGEXS_000323"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFF8011371.1"
                     /translation="MKIGLASPEKIRSWSFGEVKKPETINYRTLKPEKDGLFCERIFG
                     PTKDWECSCGKYKRVRYKGMVCDRCGVEVTKSKVRRERMGHIELAAPVSHIWYFKGIP
                     SRMGLLLDMSPRALEEVIYFASYVVVDPGPTGLEKKTLLSEAEFRDYYDKYPGQFVAK
                     MGAEGIKDLLEEIDLDEELKLLRDELESATGQRLTRAIKRLEVVESFRNSGNKPSWMI
                     LDVLPIIPPEIRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGAPGIIVQNEK
                     RMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGKRVDYSGRSV
                     IAVGPSLKMYQCGLPKEMALELFKPFVMKELVQREIATNIKNAKSKIERMDDEVWDVL
                     EEVIREHPVLLNRAPTLHRLGIQAFEPTLVEGRAIRLHPLVTTAYNADFDGDQMAVHV
                     PLSKEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTLERKDAVNTGAIFN
                     NTNEVLKAYANGFVHLHTRIGVHASSFNNPTFTEEQNKKILATSVGKIIFNEIIPDSF
                     AYINEPTQENLERKTPNRYFIDPTTLGEGGLKEYFENEELIEPFNKKFLGNIIAEVFN
                     RFSITDTSMMLDRMKDLGFKFSSKAGITVGVADIVVLPDKQQILDEHEKLVDRITKQF
                     NRGLITEEERYNAVVEIWTDAKDQIQGELMQSLDKTNPIFMMSDSGARGNASNFTQLA
                     GMRGLMAAPSGKIIELPITSSFREGLTVLEYFISTHGARKGLADTALKTADSGYLTRR
                     LVDVAQDVIVREEDCGTDRGLLVSDIKEGTEMIEPFIERIEGRYSKETIRHPETDEII
                     IRPDELITPEIAKKITDAGIEQMYIRSAFTCNARHGVCEKCYGKNLATGEKVEVGEAV
                     GTIAAQSIGEPGTQLTMRTFHTGGVAGSDITQGLPRIQEIFEARNPKGQAVITEIEGV
                     VEDIKLAKDRQQEIVVKGANETRSYLASGTSRIIVEIGQPVQRGEVLTEGSIEPKNYL
                     SVAGLNATESYLLKEVQKVYRMQGVEIDDKHVEVMVRQMLRKVRIIEAGDTKLLPGSL
                     VDIHNFTDANREAFKHRKRPATAKPVLLGITKASLETESFLSAASFQETTRVLTDAAI
                     KGKRDDLLGLKENVIIGKLIPAGTGMRRYSDVKYEKTAKPVAEVESQTEVTE"
     gene            complement(14653..18204)
                     /gene="rpoB"
                     /locus_tag="ACGEXS_000324"
     CDS             complement(14653..18204)
                     /gene="rpoB"
                     /locus_tag="ACGEXS_000324"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFF8011372.1"
                     /translation="MAGQVVQYGRHRKRRNYARISEVLELPNLIEIQTKSYEWFLREG
                     LIEMFRDISPIEDFTGNLSLEFVDYRLGEPKYDLEESKNRDATYAAPLRVKVRLIIKE
                     TGEVKEQEVFMGDFPLMTDTGTFVINGAERVIVSQLVRSPSVYFNEKIDKNGRENYDA
                     TIIPNRGAWLEYETDAKDVVYVRIDRTRKLPLTVLLRALGFSSDQEIVDLLGDNEYLR
                     NTLEKDGTENTEQALLEIYERLRPGEPPTVENAKSLLYSRFFDPKRYDLASVGRYKTN
                     KKLHLKHRLFNQKLAEPIVNTETGEIVVEEGTVLDRRKIDEIMDVLESNANSEVFELH
                     GSVIDEPVEIQSIKVYVPNDDEGRTTTVIGNAFPDSEVKCITPADIIASMSYFFNLLS
                     GIGYTDDIDHLGNRRLRSVGELLQNQFRIGLSRMERVVRERMSIQDTESITPQQLINI
                     RPVIASIKEFFGSSQLSQFMDQANPLAELTHKRRLSALGPGGLTRERAQMEVRDVHYS
                     HYGRMCPIETPEGPNIGLINSLSSYARVNEFGFIETPYRKVDLDTHAITDQIDYLTAD
                     EEDSYVVAQANSKLDENGRFMDDEVVCRFRGNNTVMAKEKMDYMDVSPKQVVSAATAC
                     IPFLENDDSNRALMGANMQRQAVPLMNPEAPFVGTGMEHVAACDSGAAITAKHRGRVE
                     HVESNEILVRRLVEENGVEHEGELDRYPLAKFKRSNSGTCYNQRPIVAVGDVVEYNEI
                     LADGPSMELGEMALGRNVVVGFMTWDGYNYEDAVIMSERLVKDDVYTSIHIEEYESEA
                     RDTKLGPEEITRDIPNVSESALKNLDDRGIVYIGAEVKDGDILVGKVTPKGVTELTAE
                     ERLLHAIFGEKAREVRDTSLRVPHGAGGIVLDVKVFNREEGDDTLSPGVNQLVRVYIV
                     QKRKIHVGDKMCGRHGNKGVISKIVPEEDMPYLPDGRPIDIMLNPLGVPSRMNIGQVL
                     ELHLGMAAKNLGIHVASPVFDGANDDDVWSTIEEAGMARDGKTVLYDGRTGEPFDNRI
                     SVGVMYMLKLAHMVDDKLHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGA
                     AYTLQEILTYKSDDTVGRVKTYEAIVKGENISRPSVPESFRVLMKELQSLGLDVKVMD
                     EQDNEIEMTDVDDDDVVERKVDLQQNDAPETQKEVTD"
     gene            complement(18419..19027)
                     /locus_tag="ACGEXS_000325"
     CDS             complement(18419..19027)
                     /locus_tag="ACGEXS_000325"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8011373.1"
                     /translation="MSHYYDEDPSVISNEQRIQYQLNHHKIDLITDNGVFSKDKVDYG
                     SDVLVQTFLKAHPPGPSKRIADVGCGYGPIGLMIAKVSPHHSITMLDVNHRALALVEK
                     NKKLNGIDNVIVKESDALSAVEDKSFDFILTNPPIRAGKETVHRIFEQALHRLDSNGE
                     LFVVIQKKQGMPSAKKRMNELFGNVEVVNKDKGYYILRSIKA"
     gene            complement(19202..19570)
                     /gene="rplL"
                     /locus_tag="ACGEXS_000326"
     CDS             complement(19202..19570)
                     /gene="rplL"
                     /locus_tag="ACGEXS_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFF8011374.1"
                     /translation="MANHEQIIEAIKEMSVLELNDLVKAIEEEFGVTAAAPVAVAGAA
                     GGADAAAEKTEFDVELTSAGSSKIKVVKAVKEATGLGLKDAKELVDGAPKVIKEALPK
                     EEAEKLKEQLEEVGATVELK"
     gene            complement(19613..20113)
                     /gene="rplJ"
                     /locus_tag="ACGEXS_000327"
     CDS             complement(19613..20113)
                     /gene="rplJ"
                     /locus_tag="ACGEXS_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFF8011375.1"
                     /translation="MSAIIEAKKQLVDEIAEVLSNSVSTVIVDYRGLTVAEVTDLRSQ
                     LREAGVEYKVYKNTMVRRAAEKAGIEGLDEFLTGPTAIATSSEDAVAAAKVISGFAKD
                     HEALEIKSGVMEGNVITAEEVKTVGSLPSHDGLVSMLLSVLQAPVRNFAYAVKAIGEQ
                     KEENAE"
     misc_feature    complement(20151..20297)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(20385..21077)
                     /gene="rplA"
                     /locus_tag="ACGEXS_000328"
     CDS             complement(20385..21077)
                     /gene="rplA"
                     /locus_tag="ACGEXS_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFF8011376.1"
                     /translation="MAKKGKKYQEAASKVDRTQHYSVEEAIKLAKETSIANFDASVEV
                     AFRLGIDTRKNDQQIRGAVVLPNGTGKSQSVLVFAKGDKIAEAEAAGADYVGEAEYVQ
                     KIQQGWFDFDVVVATPDMMGEVGKLGRVLGPKGLMPNPKTGTVTMDVKKAVEEIKAGK
                     VEYRAEKAGIVHASIGKVSFTDEQLIENFNTLQDVLAKAKPSSAKGTYFKSVAVTTTM
                     GPGVKIDTASFK"
     gene            complement(21285..21707)
                     /gene="rplK"
                     /locus_tag="ACGEXS_000329"
     CDS             complement(21285..21707)
                     /gene="rplK"
                     /locus_tag="ACGEXS_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012664221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFF8011377.1"
                     /translation="MAKKVDKVVKLQIPAGKANPAPPVGPALGQAGVNIMGFCKEFNA
                     RTQDQAGLIIPVEISVYEDRSFTFITKTPPAPVLLKKAAGIEKGSGEPNKTKVATVTK
                     DQVREIANSKMQDLNAADEEAAMRIIEGTARSMGIVVE"
     gene            complement(21888..22436)
                     /gene="nusG"
                     /locus_tag="ACGEXS_000330"
     CDS             complement(21888..22436)
                     /gene="nusG"
                     /locus_tag="ACGEXS_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFF8011378.1"
                     /translation="MSEEVGAKRWYAVHTYSGYENKVKKNLEKRVESMNMTEQIFRVV
                     IPEEEETQVKDGKAKTTVKKTFPGYVLVELIMTDESWYVVRNTPGVTGFVGSAGAGSK
                     PNPLLPEEVRFILKQMGLKEKTIDVELEVGEQVRIKSGPFANQVGEVQEIETDKFKLT
                     VLVDMFGRETPVEVEFDQIEKL"
     gene            complement(22449..22631)
                     /gene="secE"
                     /locus_tag="ACGEXS_000331"
     CDS             complement(22449..22631)
                     /gene="secE"
                     /locus_tag="ACGEXS_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFF8011379.1"
                     /translation="MAKKESFFKGVKSEMEKTSWPTKEELFKYTVIVVSTVIFFLVFF
                     YALDLGITALKNLLFG"
     gene            complement(22687..22830)
                     /gene="rpmG"
                     /locus_tag="ACGEXS_000332"
     CDS             complement(22687..22830)
                     /gene="rpmG"
                     /locus_tag="ACGEXS_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002451211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFF8011380.1"
                     /translation="MRKIPLNCEACGNRNYNVPKQEGSATRLTLKKYCPKCNAHTIHK
                     ESK"
     gene            complement(22945..23514)
                     /locus_tag="ACGEXS_000333"
     CDS             complement(22945..23514)
                     /locus_tag="ACGEXS_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFF8011381.1"
                     /translation="MKYDLTTQDSTIKRNNAINDKDFEKLVMDLQPLIIRRIKTFGFN
                     HYDLEDLYQEILIRMYRSVQTFDFSGEQPFTNYVQCLITSVKYDYLRKYLATNKRMDN
                     LINEYRVTYPCAIKRFDVENNYLNKLAIKELICQFKYLSAFEKDVMYLMCEQYKPREI
                     AQLMHVKEKVIYNAIQRCKNKIKRYFKMI"
     gene            complement(23595..24119)
                     /locus_tag="ACGEXS_000334"
     CDS             complement(23595..24119)
                     /locus_tag="ACGEXS_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFF8011382.1"
                     /translation="MKERYLIIDGYNMIGQSPTLSAIAKENLEEARMQLIDAIANYNA
                     VISDEIICVFDAYDQSGVEREYMYHGVKTIFTKEKETADSFIERYVYELYDKHTKHIT
                     VVTSDMSEQHAIFGSGAYRISSREMWRDLKENEIDVSKSLDDISENKPRTRIPLSSEI
                     LAEFEKIRRGHHKK"
     gene            complement(24119..24865)
                     /gene="rlmB"
                     /locus_tag="ACGEXS_000335"
     CDS             complement(24119..24865)
                     /gene="rlmB"
                     /locus_tag="ACGEXS_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFF8011383.1"
                     /translation="MEDTVIVGRHAVREAIITGHPINKILIQEGIKKQQINEILKNAK
                     DQKIIVQTVPKSKLDFLANAPHQGVAALIAPYEYADFDQFLKQQKEKEGLLTVLILDG
                     LEDPHNLGSILRTADATGVDGVIIPKRRSVTLTQTVAKASTGAIEHVPVIRVTNLAKT
                     IDELKDNGFWVAGTEANNATDYRNLEADMSLAIVIGSEGQGMSRLVSDKCDFYIKIPM
                     VGHVNSLNASVAASLMMYEVFRKRHDVGEI"
     gene            complement(24873..25277)
                     /locus_tag="ACGEXS_000336"
     CDS             complement(24873..25277)
                     /locus_tag="ACGEXS_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFF8011384.1"
                     /translation="MDNQQDNHIKLLNPLTLAYMGDAVLDQYVRTYIVLKLKSKPNKL
                     HQMSKKYVSAKSQAQTLEYLMEQEWFTDEEMDILKRGRNAKSHTKAKNTDVQTYRKSS
                     AIEAVIGFLYLEKREERLEALLNKIITIVNER"
     gene            complement(25270..26670)
                     /gene="cysS"
                     /locus_tag="ACGEXS_000337"
     CDS             complement(25270..26670)
                     /gene="cysS"
                     /locus_tag="ACGEXS_000337"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFF8011385.1"
                     /translation="MITLYNTLTRQKEVFKPIEPGKVKMYVCGPTVYNYIHIGNARPA
                     INYDVVRRYFEYQGYNVEYVSNFTDVDDKLIKRSQELNQSVPEIAEKYIAAFHEDVGA
                     LNVRKATSNPRVMDHMDDIIQFIKDLVDQGYAYESGGDVYFRTRKFEGYGKLSHQSID
                     DLKVGARIDAGEHKEDALDFTLWKKAKPGEISWDSPFGEGRPGWHIECSVMAFHELGP
                     TIDIHAGGSDLQFPHHENEIAQSEAHNHAPFANYWMHNGFINIDNEKMSKSLGNFILV
                     HDIIKEVDPDVLRFFMISVHYRSPINYNLELVESARSGLERIRNSYQLIEERAQIATN
                     IENQQTYIDQIDAILNRFETVMNDDFNTANAITAWYDLAKLANKYVLENTTSTEVIDK
                     FKAVYQIFSDVLGVPLKSKNADELLDEDVEKLIEERNEARKNKDFARADEIRDMLKSQ
                     NIILEDTPQGVRFKRG"
     gene            complement(26654..27301)
                     /gene="cysE"
                     /locus_tag="ACGEXS_000338"
     CDS             complement(26654..27301)
                     /gene="cysE"
                     /locus_tag="ACGEXS_000338"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFF8011386.1"
                     /translation="MILLKRMRDDIKMVFEQDPAARSTLEVITTYAGLHAVWSHLIAH
                     KLYNQKKYVAARAISQISRFFTGIEIHPGAKIGKRLFIDHGMGVVIGETCTIGDNVTI
                     YQGVTLGGTGKERGKRHPDIGDNVLIAAGAKVLGNIKINSNVNIGANSVVLQSVPSYS
                     TVVGIPGHIVKQDGVRVGKTFDHRHLPDPIYEQIKHLERQLEKTRNGEIQDDYII"
     gene            complement(27724..29178)
                     /gene="gltX"
                     /locus_tag="ACGEXS_000339"
     CDS             complement(27724..29178)
                     /gene="gltX"
                     /locus_tag="ACGEXS_000339"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFF8011387.1"
                     /translation="MSDRIRVRYAPSPTGYLHIGNARTALFNYLYAKHYNGDFVIRIE
                     DTDKKRNLEDGETSQFDNLKWLGLDWDESVDKDNGYGPYRQSERQHIYQPLIDQLLAE
                     DKAYKCYMTEEELEAEREAQIARGEMPRYGGQHAHLTEEQRQQFEAEGRQPSIRFRVP
                     QNQTYSFDDMVKGNISFDSNGIGDWVIVKKDGIPTYNFAVAIDDHYMQISDVIRGDDH
                     ISNTPKQIMIYEAFGWEPPRFGHMSLIVNEERKKLSKRDGQILQFIEQYRDLGYLPEA
                     LFNFIALLGWSPEGEEEIFSKEEFIKIFDEKRLSKSPAFFDKQKLAWVNNQYMKQKDT
                     ETVFQLALPHLIKANLIPEVPSEEDLSWGRKLIALYQKEMSYAGEIVPLSEMFFKEMP
                     ALGEEEQQVINGEQVPELMTHLFSKLEALEPFEAAEIKKTIKEVQKETGIKGKQLFMP
                     IRVAVTGQMHGPELPNTIEVLGKEKVLNRLKQYK"
     gene            complement(29735..30808)
                     /locus_tag="ACGEXS_000340"
     CDS             complement(29735..30808)
                     /locus_tag="ACGEXS_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017328539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFF8011388.1"
                     /translation="MNIVKLMVIIIYLIIGSALGIIIIPEIANDLGLQNSSFLKNHYV
                     DGIIGSIFMFLIFGVFIRRVTNAIKGLEHFIMRRSAVEILFATIGLIIGLLISVMVSF
                     ILESIGNSIFNHFIPVIITILLCYFGFQFGLKKRDEMLMFLPENIARSMSQHTKSATP
                     KIIDTSAIIDGRILEVIRCGFIDGNILIPQGVINELQIVADSNDSVKREKGKRGLDIL
                     NELYDLDYPTKVIHPTKTHSDIDTMLLKLAKQYHASIITTDFNLNKVCHVHGIKALNV
                     NDLSEAIKPNVHQGDQLHILLTKMGKEPGQAVGYLDDGTMVVVDNAKNLIGSHVNLEV
                     VSLLQTSSGRIVFAKKIEDTVSL"
     gene            complement(30833..32197)
                     /gene="radA"
                     /locus_tag="ACGEXS_000341"
     CDS             complement(30833..32197)
                     /gene="radA"
                     /locus_tag="ACGEXS_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFF8011389.1"
                     /translation="MAKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIVEKAANPK
                     HGVKTKELAGKVQKLNSIKHETTPRVLTDSAEFNRVLGGGIVSGSLVLIGGDPGIGKS
                     TLLLQICASLSQKKKVLYITGEESLSQTKLRAERLDEDSSELQVLAETDLEVIYQTVK
                     EEQPDLLVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEG
                     QIAGPRLLEHMVDTVLYFEGDEHHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVNNP
                     SEMFLEERSTNVPGSTIVATMEGTRPLLIEVQALVTPTTFNNPRRMATGIDHNRLSLL
                     MAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSVIVATASSFKDKAVDGLDCYIGE
                     VGLTGEVRRVSRIEQRVQEAAKLGFKRVIIPKNNIGGWTYPEGIQVIGVTTVHEVLSF
                     ALHS"
BASE COUNT         9653 a         6348 c         4803 g        11507 t
ORIGIN      
        1 ctgatatttc tgtaaatgga aattctagtt gtattaattg attttagtaa tttatagcgt
       61 ttattattgc taattactgt caaattaaat tttttatccc tcaactctta aactctggat
      121 atctttcatt atattagctt ttttataacc atggatatca tgtaaagcct tataagcttt
      181 aaataatgtt tcataccttt gtactttttc cgcttctgga ttaatttcgt ataaaattgg
      241 ctctttcata gcatcaactg cgtcattaat tgaattatgt gcgccaccac tgactgcacc
      301 taatattgct gcgcctattg ctggtgcata ctcactatcc ataacaatta gttttttatt
      361 cagtacattc gcatagatat ccatcataac agcattcttt ttaggtatac cgccacatgc
      421 aaataccttt tctacttcca tatgccaatc ttgatactgt tgcataatca tcttagtacc
      481 aaatgctgta gcttctaaat atgctctata aatatcctca tgcttagttt gtaaagttaa
      541 tccaaagata caacctgtta aattgctatc acttaataca cttcgatttc cattatgcca
      601 atcaagagca atgagcccac tttcacctgg catttgatgt tttatctttt cattcattaa
      661 ttcaaataca gtcatatttc tattttctgc ttcatctaca tatgactttg gtgcttgctt
      721 agcgacatac tcaaacaaat cacctactgc tgattgcccc gcttcataag caaataattc
      781 tggaataatt gctcctttta cagaacctga tatacctggc acttgatgtt gcttttcatt
      841 taacataaga tggcatgtgc ttgttcccat caccatagtc atttctttat ctttttcaga
      901 cccaatacct aataaactag catgggcatc aataataaaa ggacttacca tagtttcttt
      961 tgataatcct aatttctgtg ccattttatc atccagtttc cctactgctt caccaatatt
     1021 aacaaccggt gcagatactt tatcttgaat tacttttgat aatttggggt ctattttatc
     1081 aaataaatca taatgaaacc ctgtttcttc ttcccaaaat gctttgaaac ctaatccaca
     1141 attcgagcgt acatttttat tagttaattt atttacaatc caatcgcccg cttccataat
     1201 ataagccgtt ttttccataa tttctggtgc tcgattcatg acctccatta ttttgggaat
     1261 catccattca ctactaacat tatatccata atggcctaac cacttattat tattttcaat
     1321 agcagtttga tataatttct ctgcctcttt atatgcacca tgatgtttcc aaagtttcac
     1381 atacgcatgt ggattgtttt taaattgttt taagttatgt accgggttaa ggttttcgtc
     1441 agtaaaaata atagtagatg aagtaaagtc tataccaata cctactatat tgtctggatc
     1501 tatttttgat tcacgtacta tatatgatat tccttcttcc ataatttcta aataatcatt
     1561 actattttga agtgcatatg tatgtggtat tttcaaacca tttaattcac tctcaatgac
     1621 accatgtgta tatggtttca caaattttga tactacttga ccgttagttg tattaattaa
     1681 aaacacacgg cctgaagcag ttccataatc aattccaatg ctataagaca tcatgtttcc
     1741 tccatatcgc tagttgcggt tattagtttt gcaagcgttt tcttaaatca tactcataat
     1801 aggttctatt ttacaagtat cacttaaatt ttgccaaaaa aaaagagccc tcatacattt
     1861 acatatgagg ctcctctaat taatatttaa ttatttattt tgtattgcat ttaacatttc
     1921 attaactgct aattttccta actcatttga agctaaagga ctgtcacctg ttaataattt
     1981 acgatctttt aacgttcttc ctgtcatatc gtcgttaact acttttaatc cttgtttagt
     2041 taataaatca gcaactaacc atttcaagcg tccaggtaaa taacctattt caatatttgc
     2101 accttcatct aatgagtcag ggaagacaca aacagagtat ccttctaatg gagatttttc
     2161 tctgttaagc cctgctgaaa gtagtgctgc tggtccatga cataatgtaa ctataaagcg
     2221 gtcattgtct aatgcccaat ctaatgtttg ttgaacgtcc tcactttcag aaataccaac
     2281 aactgcagca tgtccgcctg ggataaagac agataaatag tctgaatcag gtcctaattc
     2341 atttttaatc acatctgcta atttttttgg ctgttttaat ttttctttca atttattata
     2401 agtacttata actgcctcgt cttcagttgg catagcccat aattctaatt taactggata
     2461 accagataat gtcgcaacat caacgtcaaa acctgcttcc attaaatgat gtaaaggtaa
     2521 taacatttca acaggatgat tacccgtaga gaacattttt ccattttcca ataatacata
     2581 tcgctcttca gcagcaatca tcaatacttt ccatttacca tctttatagg cacctttgtg
     2641 ttcaacacca tcaaaatctg ttttaggtgc tgtatattga ctaagggaat atggtgatgg
     2701 gaaaaatgcg ttatcttctg ctttatctgg cgttggttgc ttacttaatt catttacatc
     2761 ttgtgacata gtttattcct actttctcta tatagttact actagtatag acatatacta
     2821 cacactataa acatgtatta ctattaaaat gctcattatc aaaaataatc aaaatgaatt
     2881 tctttgaaaa aaatacaact aaattctaca agatgctttt aaaattcaaa cttcaatttt
     2941 ggggagcact aaacattgat atacgaactt actgttaata aactaaacat taaaaaaagc
     3001 ccacgacaat atttgccgtg ggaataaata aatattaaat caacttcatt tcttttccta
     3061 ctttttcata agccgcaatt gcttcatcta acatgtcttt tgtatgtgca gctgtaggca
     3121 tatttcttac acgtcctgta cctcttggta ctgttgggaa aacgatagat ttcacataga
     3181 caccttcgtc ttttaaacgc ttactaaatt cttgagttgt tttttcatca ccaataatta
     3241 ctggtgtaat tggagtttct gactcacctg tatcatatcc taattttgac aatccatttt
     3301 ttaaatattg tgcattgttc cataatttat catgtaattc agttgaatcc attaactttt
     3361 taactgcttc agttattgct ttggtatccc caggtgctaa tgatgtagag aataagaatg
     3421 gtcgtgattg tgcttttaac caatctatta actcttttgt acctgctaca taaccgccaa
     3481 cgacaccaat tgctttagaa agcgtaccta tttggaaatc gattttatct tgtaaaccaa
     3541 aatgtttaac cgtaccagcg cctttaccca taacacctga accatgagcg tcgtcaacat
     3601 aagttaataa accaaattct tctgcaattt ctacaatttc aggtaattta gccacatcac
     3661 catccatact aaaaacgcca tcagtgatat acatcacttt attgtattga cctgattcaa
     3721 ctgcttcttt tgctttcgca cgtaaatcat ccatgtctga atggttaact cgaataattt
     3781 tagctttaga taagcgacat ccatcaataa ttgatgcatg attaagctca tctgataaaa
     3841 tagcatcatt tttattcatg acagctgaaa tagcagccat attacaatta aatcctgatt
     3901 gataagctat tgcagcttct gttcctttaa attttgctag tgtttcttct aattcgtcgt
     3961 gtaaatctaa tgtaccattg attgtacgaa cagcgcctgc acctacacca tgtgtatcaa
     4021 tagctgcttt tgcagctgat ttcaaatctt catttgttgc tagtcctaaa taattatttg
     4081 aagataagtt aatgtatgat ttcccattga ttttgatttc tggtccgttt gcaccttcaa
     4141 ttgtatctat ttcattatac aaaccatttt cttttagata atttatattt tcctctaaaa
     4201 actcatgtaa tgattgaacc acagtatata atccccctta agtattgttt ggtaatacct
     4261 ttatcataca gtaattctac ataacttgaa agccttatca ttcatagata aaatatctca
     4321 aagcaatcat ttagtacgat aatttcataa aatgttatac tataaacatt attttagaaa
     4381 gtagtgataa gggtgttaga ttggttccaa ctagcaaata ataaagaaaa taaaacaatt
     4441 caactcagac gttatttaca ccaatatcct gagttatctt ttgaagaatt tcaaacacat
     4501 gattatattg ttaaccaatt aagccagtta tcttgtgata ttgaaactcc tatcggccgc
     4561 aatggtatca aagccacatt taaaggcttg ggcacaggac ctaccattgc attaagagct
     4621 gactttgatg cgttacctgt cgaggaatta aacgatgtgc cttataagtc taaaaatcca
     4681 ggatgtatgc atgcgtgtgg gcatgatggt catacagcaa ttttacttac tgtagcagag
     4741 attttagatg aacataaaca tttattagaa ggtaatgttg tattaatatt ccagtacggt
     4801 gaagaaatca tgccaggtgg ttctcaagaa atgatagatg ctggatgtct ggaaaatgta
     4861 gatagaattt acggtacaca tttatggagt ggttatccta ctggaactat tcattcacgt
     4921 gcgggggcaa tcatggcctc tccagatgaa tttagcgtca catttaaagg tcgtggtggt
     4981 catggtgcca aacctcatga aaccattgac ccaatagtaa tcatggcaga gttcatttta
     5041 agtgcacaaa aaataatttc acgtacgatc gatccagtta aacaagctgt actttcattt
     5101 ggaatgattc aagcaggcac cacagatagc gttattcctg atcaagcatt ttgtaaaggt
     5161 acagttagaa ctttcgattc agatatacag aatcatgtaa tggacaaaat ggataagctt
     5221 ctacaaggtt tagcaatagc aaatgatatt aactatgatt tgaattatat taaagggtat
     5281 ttgcctgtcc ataataacga gaaagcatat caagttatta aagaagcaac aaatgactta
     5341 catgtacgct ttaatgaatc agatttaatg atgattggag aagatttttc acattatttg
     5401 aaagtacgac caggtgcatt ctttctgact ggttgtggta atgaaagtaa aggtattaca
     5461 gcgccacacc ataatcccaa atttgatatt gatgaaaaat cattaaaata cgctgtagct
     5521 gtatttttaa aaatcataga attagagcaa gtatttaaaa caaattaagc atttaaatta
     5581 attgaaagat atgaatctga gacataaact aatgaaatgc tcccttcaaa gtagacattg
     5641 aaaaaatgaa aactttgaag ggagcatttt acaatgtcca agactcaaca attttattta
     5701 aacagtatta tcagttataa gttttattag tcagtctttc atcaattatt gttcttctaa
     5761 atcgaatagc cattacaaaa tttacacatt aaaaaaagac ccctcgattg agggatcttc
     5821 tttttatata aaatctaaga aattagaaat tatttaatga tttcagtaac aacgcctgat
     5881 cctacagtac gtccaccttc acggattgag aaacgagtac cgtcttcaat cgcgattgga
     5941 gcgattaatt ctactgtcat ttcaacgtta tcaccaggca ttaccatttc agtaccttct
     6001 ggtaagtgaa caacaccagt tacgtcagta gtacggaaat agaattgtgg acgatagttt
     6061 gagaagaatg gagtgtgacg tccaccttcg tcttttgata atacgtatac ttctgctttg
     6121 aattcagtat gtggtgtaat tgaaccagga gcagctaata cttgaccacg ttgtacgtct
     6181 tcacgagcaa caccacgtaa taatgcacca atgttgtcac cagcttcagc gtagtctaat
     6241 aatttacgga acatttcaac acctgtaaca gttgttttag atgtgtcatg taaaccgatg
     6301 atttcaactt cttcaccaac tttgatttga ccacgttcaa cacggcctgt agcaacagta
     6361 ccacgaccag tgattgagaa tacgtcctca actggcatca tgaatggttt gtcagaatca
     6421 cgttctggag ttggaatgta agtatctaca gcttccatta attctaagat tttttcttcg
     6481 tattgagcat cgccttctaa agcttttaat gctgaaccag cgattacagg tacatcgtca
     6541 cctgggaagt catattcgct taataagtca cgaacttcca tttctactaa ttctaataat
     6601 tcttcatcgt caaccatgtc aactttgttt aagaatacta ctaatgctgg tacaccaacg
     6661 ttacgtgata aaagaatgtg ttcacgagtt tgtggcattg gaccgtcagc agcagatact
     6721 actaagatac cgccgtccat ttgagcagca ccagtgatca tgtttttaac gtagtcagcg
     6781 tgtcctgggc agtcaacgtg agcgtagtga cgtttgtcag tttggtactc aatgtgagaa
     6841 gtattgattg tgataccacg ttctttttct tctggagcgt tgtcaatcat gtcatatgat
     6901 tgtgcaactg agtcaccatt ttttgctaat acagtagcga ttgctgctgt taatgttgtt
     6961 ttaccatggt caacgtgacc gatagtaccg atattggcat gttctttaga acgatcgaat
     7021 ttttcttttg ccattataaa atctctccta ttctaattaa aaataatatt taaatttatc
     7081 tctcatgata gtttctcacc atcatgagaa attatttata gcgatgcaaa aagaatttta
     7141 caattctttt ataatgcatt caccccaaaa aatcaaacta taaagatgat agcttacatt
     7201 taagctcacc ttgtatttta acctaggcta gctagtcaaa acaagttata ttattcacct
     7261 ttatttttct tgataatatc ttcagcgatt gattttggaa cttcagcata gtgatcgaag
     7321 tacatagtgt aagtaccgcg accttgagtg tttgaacgta atgatgttgc ataaccgaac
     7381 atttctgaaa gtggtacata agcattaaca acttgtgcat taccacgagg ttccatacca
     7441 tcaacacgtc cacgacgaga tgttacgtca cccatgatat cacccatgta ctcttcaggc
     7501 atttcaatag ttactttcat cattggttct aagattacag gatcacattt tttagcagct
     7561 tctttaagtg ctaatgatgc agcaattttg aaggccattt cagatgaatc gacatcatgg
     7621 tatgaaccat catataattt agctttaaca tcaattaaag gataacctgc taaaacacca
     7681 ttttccatag catctttaag accagcttct actgatggaa tgtattcacg aggaactaca
     7741 ccaccaacga tagcgttttc gaattcgaaa cctgcgcctg tttcgtttgg tgtgaattca
     7801 atgtgaacat caccgtattg accacgacca ccagattgac gagagaattt accttgaact
     7861 tgtgcagatg atttgaatgt ttcacgatat gaaaccattg gagcacctac gttacattca
     7921 acgttgaatt ctttcttcat acggtctact aagatgtcta agtgaagctc acccatacca
     7981 ccgatgataa cttgtccagt ttcttcgtca gtgtgtgcat ggaatgttgg gtcttcttct
     8041 tgtaatttaa ctaaagcttg agtcatttta tcttggtcag ctttagattt tggctctact
     8101 gataagtgaa taactggctc tgggaattcc attgattcca agataatgtc atttttctca
     8161 ccacataaag tatcaccagt acctgtatct ttaagaccta ccgcagcagc gatatctcca
     8221 gagtatacag tatcgatttc ttgacgtgag ttagcgtgca tttgtaataa acgacctaca
     8281 cgttcacgtt tacctttagt agagttctta acgtatgaac cagatgtcat tgtacctgaa
     8341 tacacacgga agaatgttaa tttaccaaca taagggtcag tcataacttt gaacgctaat
     8401 gcagcgaatt cagctgaatc gtctgctttc gcgattactt cttcttcagg gttgctagca
     8461 cggtgaccaa taattggttt aacgtctagt ggtgaaggta agtaatcaat tacagcgtca
     8521 agcattaatt gaacaccttt gtttttgaaa gctgtaccac aaagtactgg gtagaattct
     8581 acgttagtag tagcttggcg gatagcttct tttaattcag aaactgaaat ttcttcgtca
     8641 ccaagatatt tttccattaa ttcgtcgcta gtttctgcaa ctgcttcgat taagctagca
     8701 cgagcttctt cagctctatc taagtggtct tcaggaattt caatttcttc aatttcagta
     8761 cctaaatcat ttgtatattt gaaacatttc atttcaacta agtcaatgat tgcttcgaat
     8821 tcgtcttccg caccaattgg taattggatt ggagcagcgt tagcttgtaa acgatcatgt
     8881 aatgtactta cagagtattc gaagttagca cctaatttgt ccattttgtt tacaaatacg
     8941 atacgtggaa caccataagt tgtagcctga cgccaaactg tttcagtttg aggttcaaca
     9001 cctgattgtg catcaagtac tgtaactgct ccgtcaagta cacgtaatga acgttcaact
     9061 tctacagtga agtctacgtg tccaggtgta tcgataatgt ttacacggtg accttcccaa
     9121 gctgctgttg ttgcagcaga tgtgatagta ataccacggt cttgttcttg ctccatccag
     9181 tccatttgtg aagcaccttc gtgtgtttca ccaattttgt ggatacggcc agtgtaataa
     9241 agaatacgtt cagtcgtagt cgttttacca gcatcaatgt gagccatgat accgatatta
     9301 cgagtttttt ctaatgaaaa ttctctagcc atgtattttt tctccttcca gtttatatta
     9361 ctgaataaat acgatagata tggcgatgcc ctaagcatga acgcttaatg aaaattcatt
     9421 aatatagaac acactcaggg taaaagcttt tatcttacca acggtagtga gcaaatgctt
     9481 tgtttgcttc agccattttg tgagtgtcct cacgtttctt aacggcacca cctgtattat
     9541 ttgctgcatc taaaatttcg ttagctaaac gatcttccat cgttttttca ccacgaagac
     9601 gtgcatagtt aactaaccaa cgtaaaccta aagtagtacg acgctctgga cgaacttcta
     9661 ctggtacttg atagttagaa ccacctacgc gacgagcttt aacttctaat actggcataa
     9721 tgttgttgat tgcttcttcg aatacttcta atgcatcacg accactgcgt tgttcaacta
     9781 ggtcgaatgc tgaataaaga attctttgtg ctgttccacg tttaccatct aacataattt
     9841 tgttaattaa tttagttact aacttagagt tatgaattgg atctggtaat acgtctcttt
     9901 taggtactga tcctttacga ggcataatgt aaatcctccc ttccttatat taaatatttt
     9961 aagtttaaga tttaattaaa aactaaattc ttagttttta ggtttcttag ttccgtataa
    10021 tgaacgacct tgtctacgtc cgtcaacacc tgaagtatca agtgctccac gtacaatatg
    10081 gtaacgcaca cctggtaagt cttttacacg tccaccacgt acaagtacaa cactgtgttc
    10141 ttgtaagtta tgtccgatac cagggatgta tgcgttaatt tcgatgttgt ttgataaacg
    10201 cacacgtgca tatttacgta acgctgagtt aggtttttta ggtgtcattg tacctacacg
    10261 agtacataca ccacgttttt gtggtgagtt taagtcagta aattttttct ttttactgtt
    10321 gaaaccttta tttaaagctg gagaatctga ttttttgatt ttgctttgtc ttggtttacg
    10381 tactaattgg ttaatagttg gcatgtgata tgtcctcctc tcttcacttt ttaatcccac
    10441 acatccaggt ggttcatttt taggttaaat aaaatttagt aagtaaaaca cttactaatt
    10501 ctcatttaat caatgctact attgtcgcat tgacgttaat acctacatgt ttacccaaag
    10561 catgtttgct tttgaaaaaa gatacaggta tatttttctg attgatttgg cttaacacgc
    10621 gagtcattaa atatacttca acgtcttcag caataatcaa agatgtaact tgatctttct
    10681 ttaacgcttt aagcgtttct ttaagaccaa ctacaaaatg ttgtttgtta aagcgtgcaa
    10741 ctttttcctt agacaataat ttatcctcca aagttctgct tgcatcaacc tttaatatat
    10801 taacattgaa caagagaatt cgtcaacatt aaatcaaata atttacataa aaataacaag
    10861 aggctaaagc attagcctct gttatatact tgttattccg ttacttcagt ttgagattca
    10921 acttctgcaa ctggtttagc tgttttttcg tattttacgt cgctataacg tctcatacca
    10981 gtaccagctg gaattaactt accaataatt acgttttctt taagacctaa taagtcatca
    11041 cgcttacctt taattgctgc atctgtaaga actcttgttg tttcttggaa tgatgctgca
    11101 gataagaaac tttctgtttc aagtgatgct ttagtaatac caagtaatac tggtttagct
    11161 gttgcaggac gcttacggtg tttaaatgct tctctatttg catctgtaaa gttatgaata
    11221 tcaactaatg aacctggtaa taacttcgta tcacctgctt cgataattct aactttacgt
    11281 aacatttgtc gaaccataac ctcaacgtgt ttatcgtcga tttctacacc ttgcatacgg
    11341 taaacttttt gtacttcttt taataagtag ctttcagtcg cgtttaatcc agcaacagat
    11401 aagtaattct taggttcaat agaaccttca gttaatactt caccacgttg aactggttga
    11461 ccgatttcta caataattct tgaagtacct gaagcaaggt atgatcttgt ttcattagca
    11521 cctttaacaa caatttcttg ttgtctatct tttgctaatt taatatcttc tacgacacct
    11581 tcgatttccg taattaccgc ttgaccttta gggttacgtg cttcgaaaat ctcttgaata
    11641 cgaggaagac cttgtgtgat atcgctacct gctaccccac ctgtatggaa tgtacgcatt
    11701 gtaagctgtg tacctggttc accgatagat tgggctgcaa ttgtaccaac tgcttcacca
    11761 acttcaactt tttcaccagt agcaaggttt ttaccgtaac atttttcaca aacaccatgt
    11821 cgtgcgttac aagtaaatgc tgagcgaata tacatttgtt caataccagc atctgtaatt
    11881 ttcttagcaa tttcaggtgt aattaattca tcaggacgaa tgattatttc atcagtttca
    11941 ggatgacgaa ttgtttcttt agaataacga ccttcaatac gttcgataaa tggttcaatc
    12001 atttctgtac cttctttaat atcagaaact aataaacctc tatcagtacc acagtcttct
    12061 tcacgaacaa taacatcttg tgccacgtca acaagacgac gagtaagata tcctgagtca
    12121 gctgttttaa gtgctgtatc ggcaagacct ttacgtgcac cgtgagttga gatgaagtat
    12181 tcaagtactg ttaaaccttc acggaatgaa gatgtgattg gtaattcgat aatcttacca
    12241 gatggtgcgg ccatcaatcc acgcatacct gctaactgtg taaagttaga tgcgttacca
    12301 cgggcacctg aatcactcat catgaagatt gggttagttt tatcaagtga ttgcatcaat
    12361 tcaccttgaa tttgatcttt tgcatctgtc caaatttcaa caactgcatt atatctttct
    12421 tcttcagtga ttaaaccacg gttgaattgt tttgtaattc tgtcgactaa tttttcatgc
    12481 tcatcaagta tttgttgctt atcaggtaat actacgatat cagcaacacc tactgtaata
    12541 ccagctttag atgagaattt gaatcctaag tctttcatac ggtctaacat cattgatgta
    12601 tcagtgatgc taaatctgtt gaatacttct gcaataatat tacctaagaa ttttttgttg
    12661 aaaggttcaa ttaattcttc attttcaaag tattctttta atccaccttc acctaaagtt
    12721 gtaggatcga tgaaatatct gtttggtgtc tttctttcta agttttcttg cgtaggttca
    12781 ttaatataag caaatgaatc tggaatgatt tcattgaata taattttacc tactgacgta
    12841 gcaagaatct ttttgttttg ttcttcagta aatgttggat tattgaacga acttgcatgt
    12901 acaccaattc tagtgtgtaa atgtacaaag ccatttgcat atgcttttaa tacttcattt
    12961 gtattattaa agattgcgcc tgtatttact gcatcttttc tttctaaagt aaggtaatag
    13021 ttaccaagta ccatatcttg tgatggtgta actacaggtt taccatcttt agggttcaag
    13081 atgttttgtg ctgctaacat caacattctt gcttcagctt gtgcctcttt tgataaagga
    13141 acgtgaaccg ccatttggtc accgtcaaag tcagcgttat aagctgttgt tacaagtgga
    13201 tgtagacgaa tcgcacgacc ttcaactaaa gttggttcaa atgcttgaat accaagtcta
    13261 tgaagtgttg gtgcacggtt aagtaataca ggatgttctc taattacttc ttccaatacg
    13321 tcccaaactt catcatccat acgttcgatt ttactcttcg catttttaat gttagttgca
    13381 atttcacgtt gaactaattc tttcattacg aatggtttaa atagttcaag tgccatttct
    13441 tttggtaaac cacattggta cattttcaag cttggaccta ctgcaataac tgaacgtcct
    13501 gaatagtcaa cacgtttacc aagtaagttt tgacggaaac gaccttgttt accttttaac
    13561 atatgagata aagattttaa tggacggtta cctgggccag taactggacg accacgacga
    13621 ccattatcaa ttaaagcgtc aacggcttct tgtaacatac gtttttcgtt ttgaacgatg
    13681 ataccaggtg cacctaaatc taataaacgt ttcaaacgat tatttcgatt aattacacga
    13741 cggtataagt cgtttaagtc acttgttgca aatcgtccac catctaattg aaccattgga
    13801 cgaatttctg gtgggatgat tggaagtaca tctaaaatca tccatgaagg tttgttacct
    13861 gaattacgga atgattcaac aacttctaaa cgtttaattg cacgagtaag tctttgacca
    13921 gtagctgatt ccaactcatc gcgtaacaat ttaagttctt cgtcaagatc aatctcttca
    13981 agtaaatctt taataccttc tgcacccatt tttgcaacga attgacctgg gtatttatca
    14041 taataatctc tgaattcagc ttcagataat aaagttttct tttctaaacc agttggacct
    14101 ggatctacaa caacataaga agcaaagtaa ataacttctt ctaatgctct tggtgacatg
    14161 tcaagtaata atcccatacg acttggtata cctttgaaat accaaatgtg agaaactgga
    14221 gcagcaagtt caatgtgacc cattctttca cgacgtactt tagatttagt tacttcaact
    14281 ccacatctgt cacagaccat gcctttgtag cgaacacgtt tgtatttacc acaactacat
    14341 tcccagtctt ttgtaggtcc gaaaattctt tcacagaata gaccatcttt ttcaggtttt
    14401 aatgtacggt agttgattgt ttcaggtttt ttaacttcac caaaagacca agaacggatt
    14461 ttttcaggtg aagccaatcc tattttcata taatggaaat tatttacatc aatcaaggag
    14521 cctacctcct tcaatttaga ttagctgtgc tatttgattg atttgcattt atttaaaatg
    14581 gcttaaagta ctaaactgaa tcatcaataa aattcagctt agtatctttt tgcctgtttt
    14641 gtaaattgcg tattaatcag taacttcttt ttgtgtttca ggagcatcat tttgttgtaa
    14701 atctactttg cgttctacaa catcatcgtc atcaacgtct gtcatttcga tttcattatc
    14761 ttgctcatcc ataactttta catctaaacc taaactttgt aattctttca tcaatactcg
    14821 gaatgattct ggaacacttg gtctagagat gttttcacct ttaacaatag cctcgtatgt
    14881 tttcacacgt cctactgtat catcggattt gtaagttaag atttcttgta atgtgtatgc
    14941 agcaccatat gcttcaagtg cccatacctc catctcacca aaacgttgtc caccgaattg
    15001 cgctttaccg ccaagtggtt gttgtgtaac aagtgaatat ggtcctgttg aacgcgcatg
    15061 taatttatca tcaaccatgt gcgcaagttt caacatgtac attacaccta ctgaaatacg
    15121 gttatcgaat ggttcacctg tacgtccatc ataaagtaca gttttaccat cacgagccat
    15181 accagcttct tcaattgttg accatacatc gtcatcgttt gcaccgtcaa atactggtga
    15241 tgcaacgtga ataccaagat ttttagcagc catacctaag tgtagctcta atacttgtcc
    15301 gatgttcata cgagatggta caccaagagg atttaacatg atatcgatcg gacgtccatc
    15361 tggtaagtaa ggcatatctt cttcaggaac aatcttagaa atgacacctt tgttaccatg
    15421 tcgaccacac atcttatcac caacatgaat tttacgtttt tgaacgatat atacacgtac
    15481 taattggttt acaccaggtg ataatgtatc gtcgccttct tcacgattga atacttttac
    15541 atcaagaacg ataccgccag cgccgtgagg tacacgtaat gaagtatctc taacttcacg
    15601 tgctttttca ccaaagattg catgtaacaa tctttcttcg gcagttaact cagttacacc
    15661 tttaggcgtt actttaccaa ctaaaatatc tccatctttt acttctgcac caatataaac
    15721 gataccacga tcgtctaagt tcttaagtgc actttcagaa acattaggaa tatctcttgt
    15781 gatttcttca ggtcctaact tagtatcacg tgcttctgat tcatactctt caatatgaat
    15841 agaagtatac acgtcatctt tcacaagtct ttcactcatg ataacggcat cctcatagtt
    15901 gtaaccgtcc caagtcatga aaccaactac tacgtttcta cctaatgcca tttctcctaa
    15961 ttccatagat ggtccatctg ctaaaatctc gttatactca acaacatctc caactgcaac
    16021 gattggacgt tggttgtaac atgtacctga gtttgaacgt ttaaatttag ctaatggata
    16081 gcgatctaat tcaccttcat gctcaacgcc gttctcttca actagacgac gaacaagaat
    16141 ttcattagat tcaacatgtt caacacgacc tctgtgctta gctgtaatag ccgcaccaga
    16201 atcacatgct gcaacgtgtt ccatacctgt accaacaaat ggtgcttctg gattcatcaa
    16261 aggcactgct tgacgttgca tgttcgcacc catcaatgca cggtttgagt catcattttc
    16321 taagaatgga atacatgctg tcgctgctga aacaacttgc ttcggcgata catccatata
    16381 atccattttt tctttagcca taactgtatt gttaccacgg aaacgacata caacttcatc
    16441 atccatgaaa cgaccatttt catctaattt agagtttgct tgtgctacaa catagctatc
    16501 ttcttcgtca gctgttaaat agtcaatttg atcagtgata gcatgtgtat ctaaatcaac
    16561 tttacgatat ggtgtttcaa taaagccgaa ttcatttaca cgtgcataac ttgataatga
    16621 gttaatcaat ccaatgtttg gtccctcagg tgtttcaatt ggacacatac ggccatagtg
    16681 agagtagtga acgtcacgta cttccatttg agcacgttca cgtgttaaac caccaggtcc
    16741 taatgctgat agacgacgtt tatgcgttaa ctcagctaat gggtttgctt ggtccatgaa
    16801 ttgtgataat tgagagctac caaagaattc tttaatagat gcaataacag gtcgaatatt
    16861 aattaattgt tgaggtgtga tagactcagt atcttgaatt gacattcttt cacgtacaac
    16921 tctttccatt cttgataaac cgatacggaa ttggttttgt agtaattcac ctacagaacg
    16981 taaacgacgg ttacctaaat ggtcaatatc atctgtatat ccaataccgc ttaataagtt
    17041 aaagaagtaa ctcattgaag caatgatatc tgctggtgta atgcatttaa cttctgagtc
    17101 agggaaagca ttaccaatta cagttgtcgt acgaccttca tcatcgttag gaacatatac
    17161 tttaattgat tgaatttcta ctggctcgtc tataacgcta ccatgcaatt caaacacttc
    17221 gctgtttgca tttgattcaa gtacatccat gatttcgtcg attttacgac gatcaagcac
    17281 tgtaccttct tcaactacaa tttcaccagt ttcagtattt acaattggct cagctaattt
    17341 ttgattaaat aaacgatgtt ttaaatgtaa ttttttgttt gttttataac gacccacgct
    17401 tgctaagtca tagcgttttg gatcaaagaa acgtgaatac aatagacttt tagcattttc
    17461 aacagttggt ggttcacctg gacgtaaacg ttcatagatt tctaataacg cttgttcagt
    17521 gttttcagtg ccgtctttct ctaaagtatt acgtaaatat tcattgtcac ctaaaaggtc
    17581 aacaatttct tggtcgcttg agaaacctaa tgcacgtaac aatactgtta atggtagttt
    17641 acgtgttcta tcaatacgta cgtatacaac atctttagca tctgtttcat attctaacca
    17701 tgcaccacgg tttggaataa ttgttgcatc atagttttca cgaccatttt tgtcgatttt
    17761 ttcattgaaa taaacggatg gtgaacgaac taattgagat acgattacac gttctgcacc
    17821 attgataacg aacgtacctg tatcagtcat taatgggaaa tcacccataa agacttcttg
    17881 ttctttaact tctcctgttt ctttaatgat tagacgcact tttacacgaa gaggtgcagc
    17941 ataagtagcg tcacggtttt tagattcttc taaatcatat tttggttctc ctaaacggta
    18001 atccacaaac tctaatgaca aattaccagt aaaatcttca attggagaaa tgtctctaaa
    18061 catttcgatt aaaccttctc ttaggaacca ctcgtaagat ttagtttgaa tttctattaa
    18121 gtttggtaat tctaatactt ctgaaattct cgcgtagttt ctacgtttac gatgtcttcc
    18181 atattggaca acttgacctg ccaaacagat tcacccctca aaaattatgt gatccgcttt
    18241 tagctaaata ccataagcat attaataaaa agacaaaaag aaaacggcaa cactaatgat
    18301 gacaccattt tcgattctat atttattctt aattaactta tctatttaac gtaaaagtac
    18361 acacttattg aacataaatt tttacattat ataactataa cagaatatcc atttcatttc
    18421 aagcttttat acttctcaga atgtaatatc ctttatcttt atttaccact tctacatttc
    18481 caaaaagttc attcattctt ttctttgcag atggcatacc ttgcttcttc tgaattacaa
    18541 cgaatagttc accgttcgag tctaatctat gtaatgcttg ctcgaatata cgatgcacgg
    18601 tttctttccc tgctcttatt ggtggattgg ttaaaataaa atcaaaactt ttgtcttcca
    18661 cagcagacaa agcatcactt tcctttacga tcacattatc aataccattt aattttttgt
    18721 ttttttcaac taaggctagc gctctgtgat taacatctag cattgtaatt gaatgatgtg
    18781 gtgatacttt agcaatcatc aaaccaattg gtccgtaacc acaaccaaca tcggcaattc
    18841 gcttacttgg accaggtgga tgcgctttta aaaaagtttg aacaagaaca tctgaaccat
    18901 aatctacttt atctttcgaa aacactccgt tatcagttat taaatcaatt ttatgatggt
    18961 ttaattgata ttgaatacgt tgttcattgc taattacact tggatcttca tcgtaataat
    19021 gactcattat tccacctcat cttcactttg taactcgtca ttataattat aacatttcac
    19081 tcagtcttta aatgaataaa accccgttat caaaataacg gggttttaac ttctcaaaga
    19141 atcattatat tatagcaata cttgctataa aactttgatt attaagttta agatacttga
    19201 attattttaa ttctacagta gctccaactt cttctaattg ttctttaagt ttttcagctt
    19261 cttctttagg taaagcttct ttgattactt taggagctcc gtctactaat tctttagcat
    19321 cttttaatcc taaaccagtt gcttctttaa cagctttaac aactttgatt ttagatgaac
    19381 cagctgaagt taactcaacg tcaaattcag ttttttctgc tgcagcgtca gcgccaccag
    19441 ctgcacctgc tactgctact ggagcagctg cagttacacc aaattcttct tcaattgctt
    19501 ttactaagtc gtttaattct aatactgaca tttctttaat cgcttcaatg atttgttcat
    19561 gattagccat tttataattc ctccattatt tttaattttt ttacgctaaa aattattcag
    19621 cgttttcttc tttttgttct ccaatagctt taaccgcata agcgaagttg cgtacaggag
    19681 cttgtaatac tgataaaagc atagatacaa gaccatcgtg tgaaggtaat gaaccaacag
    19741 ttttaacttc ttctgctgta ataacattgc cttccataac gcctgattta atttctaatg
    19801 cttcatgatc tttagcaaat ccagaaatta ctttcgctgc agctacagca tcttcacttg
    19861 aagttgcaat agcagtagga cctgttaaga attcatctaa gccttcgata ccagcttttt
    19921 cagctgcacg acgtaccata gtgtttttgt atactttata ctcaacacca gcttcacgta
    19981 attgtgaacg taagtcagta acttcagcta ctgttaatcc acggtagtca acgattactg
    20041 ttgaaactga atttgatagt acctcagcaa tttcatcaac tagttgtttt ttagcttcaa
    20101 tgatagcaga cattcagaca cctccattta aatttttggt gcttttttta aaattaataa
    20161 aaaaagcact ttctacccac ggcaaaaagt gcttgaaaga tcatagatca tcacgttcaa
    20221 gtcaatttta gcctcggcag gataaatttt aagttataaa taactcctac tgtcttaggt
    20281 aaaataatca caataagtaa tataacttgg aaattactta ttgtcaatat atttttacag
    20341 actgaaagat atttctttca gcctgtaatt gtttatatca tttattattt gaaacttgca
    20401 gtatcaattt taactccagg acccattgtt gtagttacag caacagattt gaagtatgta
    20461 cctttagcag atgatggttt agctttagct aatacatctt gtaaagtatt gaagttttca
    20521 attaattgtt catcagtaaa tgaaacttta ccaattgatg catgtacgat accagctttt
    20581 tcagcacgat attctacttt accagctttg atttcttcaa cagctttttt aacatccatt
    20641 gttacagttc cagttttagg gtttggcatt aaaccttttg gtcctaatac acgacctaat
    20701 ttaccaactt cacccatcat gtctggtgta gcaactacta catcgaagtc gaaccaacct
    20761 tgttggattt tttgaacgta ttctgcttca cctacatagt cagcacctgc tgcttcagct
    20821 tcagcaattt tgtcaccttt agcgaatact aatacacttt gtgatttacc agttccgttt
    20881 ggtaatacaa ctgcaccacg gatttgttgg tcatttttac gtgtatcaat tcctaaacgg
    20941 aatgcaactt caacagaagc gtcaaagtta gcaatgcttg tttctttagc taatttaatt
    21001 gcttcttcaa cactgtagtg ctgagtacgg tcaactttac tagctgcttc ttgatacttt
    21061 ttaccttttt tagccattta tagttcctcc tttagtggtt ttagcggaat ttcctcccac
    21121 attacttgct atatgcaagt taagagcaga caacagaaga attaatctta tgaataaatc
    21181 aatcattaat ctagtcattc agattatatt tataattcat cattattatc tctgttatct
    21241 gcttcatcgt ttaaatcatg taatcaaatt taatattcgt aaaattattc tacaacgata
    21301 cccatactac gtgcagtacc ttcgataata cgcatagctg cttcttcgtc agcagcgttt
    21361 aagtcttgca ttttgctgtt agcaatttcg cgtacttgat ctttagttac tgtagcaact
    21421 ttagttttgt ttggttcgcc tgaacctttt tcaataccag ctgctttttt aagtaatact
    21481 ggagccggtg gagtttttgt aataaatgta aatgaacgat cttcataaac actgatttct
    21541 accggaataa ttaaacctgc ttgatcttga gtacgtgcat tgaactcttt acagaatccc
    21601 atgatgttca cacctgcttg acctaatgct ggaccaactg gtggtgctgg attcgcttta
    21661 cctgcaggaa tttgtaattt aacaacttta tctacttttt tagccacgat gtgcacctcc
    21721 ttgatatcgt gatgtggtca cagggctcag ttttgccctc ccactcttaa catttcgtga
    21781 cgaaatgggc gctctaaaag cgcgaccaca taattttaac acttttctaa ttaaaaaatc
    21841 aatactcttt tttatctttt ggttagttta ataactttaa ttgttaatta aagcttttct
    21901 atttgatcga attcaacttc tactggtgtt tctcggccaa acatatctac taatactgtt
    21961 agcttaaact tatctgtttc aatttcttga acttcaccaa cttgattcgc aaatggacct
    22021 gatttaatac gaacttgctc gccaacttcg agttcaacat cgatagtctt ttctttaaga
    22081 cccatttgtt ttaagatgaa gcgaacttct tctggtaaca atggatttgg cttagaccct
    22141 gcacctgcag aacctacaaa accagtaacg cctggtgtat ttcttaccac ataccatgat
    22201 tcatctgtca tgattaattc cactaaaaca tatccaggga atgttttttt aacagtcgtt
    22261 ttagctttgc catcttttac ttgagtttct tcttcttccg gtatgactac tctaaagatt
    22321 tgttcagtca tattcataga ttctactctt ttttctaaat tctttttaac tttattttca
    22381 tatccagaat atgtatgcac tgcataccaa cgctttgcgc caacttcttc agacatgtct
    22441 tcactcctct aaccaaataa taaatttttc aatgctgtaa ttcctaaatc taaggcatag
    22501 aaaaagacta agaagaatat aacagtagaa actacaatta cagtatattt aaatagctct
    22561 tctttcgtcg gccaacttgt tttttccatt tcagacttaa cgcctttaaa gaaactttct
    22621 tttttagcca tctgctcaac ctccaaatcc tttgaacata ttatcaaagt attatttcga
    22681 atgtatttat ttcgattctt tatgaattgt gtgcgcgtta cattttggac aatatttctt
    22741 taaggttaat cttgttgccg agccttcttg cttaggaaca ttataatttc tattgccaca
    22801 agcttcacaa tttaaaggta tttttctcac aataattcac ctttattcat tataatacca
    22861 gtatactata cataaaattt ataacaaatg tcaaaccatc agtaagtaac acgttataat
    22921 tcaattcacg tcctaaggcg cttttcaaat cattttgaaa taacgtttta ttttattttt
    22981 acatcgttgt atggcattat aaatcacttt ctcttttaca tgcatcaatt gagcaatttc
    23041 tctcggctta tattgttcac acattaaata catgacatct ttttcaaatg cactcaaata
    23101 cttaaactga caaatcaact ctttaattgc taatttattc aaataattgt tttcaacatc
    23161 aaaacgcttt attgcacatg gatacgtaac tctatattca ttaatcaaat tatccattct
    23221 tttatttgta gctaaatatt ttctcaaata atcatacttt acagacgtaa ttaaacattg
    23281 aacataattt gtgaaaggct gctctccact aaaatcaaat gtttggaccg acctatacat
    23341 ccgtataagt atttcttgat ataagtcttc taaatcataa tgattaaatc caaatgtttt
    23401 gatgcgtcga ataattaatg gttgtagatc cattactaac ttttcgaagt ctttatcatt
    23461 tattgcattg ttacgtttga ttgtactgtc ttgagttgtc aaatcgtatt tcaaatatta
    23521 aactaacccc ttctatctaa aatttaaggt tagtttaata ttgttacatt caaaatttca
    23581 agatgacgga aatgtcattt cttatgatgt cctcttcgta ttttttcaaa ttctgcaagg
    23641 atttcagaag ataacggaat tcgagttctt ggcttgtttt cacttatatc atctaatgat
    23701 ttactcacat caatttcatt ttcttttaaa tctctccaca tttcgcgaga tgatattcta
    23761 tatgcacctg atccaaagat agcatgttgc tcactcatat cacttgttac aactgtaata
    23821 tgcttagtat gcttgtcata aagttcataa acataacgtt ctatgaaact atcagctgtt
    23881 tctttttcct tggtaaaaat cgttttaacg ccatgataca tgtattctct ttcaacaccc
    23941 gattggtcat aagcatcgaa aacacaaata atttcatctg aaataactgc attataattt
    24001 gcaattgcat ctattaattg cattctagct tcttctaaat tctcttttgc aatggcgctt
    24061 agcgttggtg attgtcctat catattgtat ccatcaatga ttaagtaacg ttctttcatt
    24121 atatttctcc aacatcatgt ctttttcgaa atacttcgta catcattaaa cttgctgcaa
    24181 ccgaagcatt caaactgttt acatgtccaa ccattggaat cttaatataa aaatcgcatt
    24241 tatcacttac taggcgactc ataccctgtc cttcgctacc aattacaata gccaatgaca
    24301 tgtccgcttc tagatttcta taatctgttg cattattagc ttcagtgcca gctacccaaa
    24361 agccattatc ttttagttca tcgatagttt tagctaaatt tgtcactcga ataactggta
    24421 catgttcaat tgcacctgtt gaggcttttg caactgtttg cgttagtgta actgaacgac
    24481 gtttaggaat aataacacca tcaactcccg ttgcatcggc tgttcttaaa attgatccca
    24541 agttatgtgg gtcttctaag ccgtctaata taagtactgt caataaacct tctttttctt
    24601 tttgctgttt taaaaattga tcgaagtcag catattcata tggtgcaata agcgctgcaa
    24661 caccctgatg tggtgcattt gctaaaaaat ctaatttaga ttttggtaca gtttgaacaa
    24721 tgattttttg atcttttgca ttttttaaaa tttcattaat ttgttgcttt ttaatacctt
    24781 cttgaatcaa tatcttattt atcggatgcc cagtaataat cgcttctcta acagcatgcc
    24841 taccaacaat aaccgtatct tccacatcgt cactaccttt cgtttactat tgttattatt
    24901 ttatttaata atgcctctaa tcgttcttct cttttttcta aataaagaaa acctatcact
    24961 gcttctatcg ctgaactttt acgatatgtt tgaacatcag tgtttttagc tttagtatga
    25021 cttttcgcgt tacgccctcg cttcaaaata tccatttctt cgtctgtaaa ccattcttgc
    25081 tccattaaat attctaacgt ttgcgcctga cttttggcag atacatattt tttagacatt
    25141 tgatgtagtt tattaggctt acttttaagc tttaaaacga tataggtacg tacatattga
    25201 tctaagactg cgtctcccat atatgctaag gtcaatggat tcaataattt aatgtgatta
    25261 tcttgttgat tatccacgtt taaatctaac cccttgaggt gtgtcttcta atataatgtt
    25321 ttgtgatttc agcatgtctc gaatttcatc tgctcgtgca aaatctttgt ttttccttgc
    25381 ttcattacgc tcttcgatta atttttcaac atcttcatcc aataattcat ctgcattttt
    25441 agattttaac ggtacaccta aaacatcgct gaaaatttga taaactgctt taaatttatc
    25501 aattacttct gttgatgttg tgttctctag tacatattta ttcgcaagtt ttgctaaatc
    25561 ataccaagct gtaattgcat tagctgtatt aaaatcatca ttcataactg tttcaaaacg
    25621 atttaaaatc gcatcaattt gatcaatata tgtctgttga ttttcaatat tagtagcaat
    25681 ttgtgcgcgc tcttcaatta attgataact attgcgaata cgctctagtc cactacgtgc
    25741 tgattctacc aattctagat tatagttaat tgggcttcta taatgtacgc taatcataaa
    25801 gaatcttagt acatctggat caacttcttt aataatatcg tgaactaaaa taaagttgcc
    25861 tagtgattta ctcatttttt cattatcaat attaatgaaa ccattatgca tccaataatt
    25921 agcaaatggc gcatgattat gtgcttctga ttgtgctatt tcattttcat gatgtggaaa
    25981 ttgtaaatct gaaccacccg catgtatatc aattgtaggt cctagctcat gaaatgccat
    26041 tacagaacat tctatatgcc atcctggtct accttcacca aatgggctat cccaactaat
    26101 ctcgccaggc ttcgcttttt tccacaatgt aaaatcaagt gcatcttctt tatgctctcc
    26161 tgcatctata cgagcaccca cttttaagtc atctatggat tgatgactta atttaccata
    26221 accttcaaat ttacgtgttc taaagtaaac atcgccacca ctttcatatg cataaccttg
    26281 atccaccaaa tctttaataa attgaataat gtcatccata tggtccatta cccttggatt
    26341 tgaagtcgct tttctaacat ttaacgcacc aacatcttca tgaaaagcag cgatatattt
    26401 ttctgcaatt tcgggaacag actgatttaa ttcttgagaa cgtttaatta atttatcatc
    26461 tacgtctgta aaatttgata catattctac attatatcct tggtattcaa agtaacgtct
    26521 cactacgtca taattaattg ctggtcttgc gttaccaata tgaatgtagt tatatacagt
    26581 aggaccacat acatacattt ttactttccc tggttctata ggcttgaaca cttctttttg
    26641 acgtgtaagc gtattatata atgtaatcat cttgaatctc tccattccta gtcttttcaa
    26701 gttgtcgttc taaatgctta atttgttcat aaattggatc aggtagatgg cgatgatcaa
    26761 atgtttttcc aactcgaaca ccatcttgct taacaatatg tcctggtata ccaacaaccg
    26821 ttgaataact tggaactgat tgtaaaacaa ctgaatttgc accaatattt acatttgaat
    26881 ttattttaat atttcctaaa actttcgcac cggctgctat taaaacattg tctcctatat
    26941 ctgggtgtct tttccctctt tctttccctg tcccaccaag tgtcacgcct tgatagattg
    27001 tcacattatc accaattgta catgtttctc ctattacaac gcccatacca tgatctataa
    27061 atagacgctt tccaatttta gcacctggat ggatttctat acctgtgaaa aatcttgaaa
    27121 tttgagatat cgcgcgtgct gcaacatatt ttttttggtt gtataactta tgtgcaatca
    27181 aatgactcca aactgcatgt aaacctgcat acgttgtaat gacttctaat gttgaacgtg
    27241 ccgctggatc ctgctcaaat accattttta tatcgtctct cattcttttt aacaagatca
    27301 tttcctcctc aatgattgaa ctacgtaaat acataattga agtacctgcg aaattaaata
    27361 tcaaaaaagc accactaaca tacaaattgt attgttagag gcgcttccgc acggttccac
    27421 tctgaattta gcgaataaca ttaataatat tgcgggcgct tccaaattat caaggaaact
    27481 aagtcaactt aatgctcatc actctcatta tatatttaat tcattttacg aaggtgcatt
    27541 cattaatttc tacgttgtac tcacagcaac cgtacactct ctgcatcgta taaatttaat
    27601 tactaatcct tcgttttata taaaatactt tattcatatt gttgtaattt gttaatacaa
    27661 ctttatcata tgtggttacg ttataaaatt caagtatata ctaccttgat cttgtctatt
    27721 tcattactta tattgtttta aacggtttag cactttttct ttaccaagta cttcaattgt
    27781 atttggtaat tcaggaccat gcatttggcc agttacagca acacgaatag gcataaataa
    27841 ttgcttgcct tttattcctg tttctttttg aacttcttta attgtctttt taatttcagc
    27901 cgcttcaaat ggttcaagtg cttctaattt actgaataag tgcgtcatta actctggtac
    27961 ttgctctcca ttaatcactt gttgttcttc ttcaccaaga gctggcattt ctttaaagaa
    28021 catttctgat aaaggtacaa tttcaccggc ataactcatt tctttttgat aaagcgcaat
    28081 taatttgcgt ccccaagata aatcctcttc tgacggcacc tcaggaatca aatttgcttt
    28141 aattaaatga ggtaatgcta attggaatac tgtttcagta tctttttgtt tcatatattg
    28201 gttattaacc catgctaatt tttgcttatc gaaaaatgct ggtgattttg acaaacgctt
    28261 ttcatcaaag attttgataa attcttcttt agaaaagatt tcttcttcac cttcaggaga
    28321 ccaacctaat aacgcaataa aattaaataa cgcttcaggt aaataaccta agtcacgata
    28381 ttgctcaata aattgtaaaa tttgcccatc acgtttactt aactttttac gttcttcatt
    28441 aacaattaat gacatatgac caaaacgagg tggctcccag ccaaatgctt cataaatcat
    28501 aatttgttta ggcgtgtttg aaatatgatc atcaccacga attacatctg aaatttgcat
    28561 gtaatgatca tctatagcta ctgcaaaatt gtacgttgga atgccatctt tttttacgat
    28621 aacccagtca ccaataccat ttgaatcaaa tgaaatattt ccttttacca tatcatcaaa
    28681 tgaatacgtt tggttttgag gtactcggaa acgaattgat ggttggcgtc cttctgcttc
    28741 aaattgttga cgttgttctt cagtcaaatg cgcatgttga ccaccatagc gaggcatttc
    28801 accacgagcg atttgcgctt cacgttcagc ttctaattct tcttctgtca tatagcattt
    28861 atatgcttta tcttctgcta gtaactgatc tattaatggt tggtagatat gttgacgttc
    28921 agattgacga tatggtccgt agccattgtc tttatctaca gactcatccc aatctaatcc
    28981 taaccattta agattatcaa attgtgatgt ttctccatct tctaaattac gttttttatc
    29041 agtatcttca attcgaatca caaaatctcc gttgtaatgt ttagcataca agtaattgaa
    29101 taatgctgtt cttgcattac caatatgaag atacccagtt ggacttggtg catatcttac
    29161 tcttatacga tcgctcattt ttttcactcc taaattaaat atcagatttt caagttagtt
    29221 catataaatt gttcatttgc tatcttcgac cgtcataaca aatgtctaac tcgtcttatt
    29281 gttaaaacga aacaatgctt tttaacatga ccttaaaata atttcattgt ttaatcataa
    29341 cataattccc tgggtaatat gcttaaattt taaatagaaa gctgttgttt tttcaacact
    29401 ttaaaaaagc tatccctaag aatcaagact gcactacata aacatttcaa cgacttaaaa
    29461 atgaatatta tgtactgtga aattaaactt caaattaact attcaaatac gttaaaattg
    29521 attctaattt tgtatgtctt gattgctata agaataactt tattaatatc taaaatttaa
    29581 cacttaatga acttgtttca atgatatatt agcactattt gtattttttg ataactaata
    29641 tgttttgcat ttatttatag ttatacttca aattacaaac ttcgccattt catatacctt
    29701 ttaatatcta ttttgttttc gtcaactaca gtttttataa tgatactgta tcttcgattt
    29761 ttttagcaaa aacaattctt cctgaagatg tttgcaataa gctgactact tctaaattga
    29821 catgactgcc aataagattt ttagcattat caacaactac catcgtacca tcatctagat
    29881 atcctactgc ctgaccaggc tccttaccca tttttgtcag taaaatatgc agttgatcac
    29941 cttgatgtac attaggtttg attgcttctg ataaatcatt aacatttaat gctttgatac
    30001 catgtacatg acaaacttta tttaggttga aatctgtcgt tataatactt gcatgatatt
    30061 gttttgctaa ttttaataac atcgtatcaa tatcactatg tgttttagtt ggatgtataa
    30121 cctttgtagg atagtctaaa tcatacaatt catttaaaat atctaagcct cttttaccct
    30181 tttcacgttt aacactgtca tttgaatctg caacaatttg taattcatta ataacacctt
    30241 gtggaattaa aatattgcca tcgataaaac cgcaacgaat gacttctaaa atacgaccat
    30301 caataattgc gcttgtgtcg ataatttttg gcgtagcact tttagtatgt tgtgacatgg
    30361 aacgcgctat attctcaggt aaaaacatta acatttcatc tcgtttttta aggccaaatt
    30421 ggaaaccgaa ataacatagt aatatcgtaa ttatgacagg aatgaaatga ttaaaaatag
    30481 agttgccaat tgattctaat ataaacgaca ccataacaga aataagtaat ccgattatta
    30541 aacctattgt tgcgaatagt atttcaacag cacttctacg cataataaaa tgttctaaac
    30601 cttttatagc gttagtaact cgtctaataa atacaccaaa aattaagaac ataaaaatac
    30661 taccgataat gccatctaca tagtgatttt ttaaaaagct ggagttttgt aatccaagat
    30721 catttgcaat ttcaggaata ataattattc ctaatgcgct cccaataatt aagtaaataa
    30781 taataaccat tagtttaacg atattcacac aatgtcctcc tttcttgatg ttttatgaat
    30841 gaagagcaaa tgacaatact tcatgtacag tagttacacc tattacttgt ataccttcag
    30901 gatatgtcca tccgcctata ttatttttag gaataattac acgtttgaaa cctagttttg
    30961 cagcctcttg cacgcgttgt tctatccgag atacacgacg tacctcaccc gttaaaccaa
    31021 cttctccaat atagcaatct aatccgtcga cagctttatc tttaaagcta gatgcagttg
    31081 ctacaattac acttaaatca actgctggct ccgttaactt tacaccgcca gctactttga
    31141 tataagcatc ttgttgttgt aatagataat tttctttctt ttccaaaaca gccatcaaca
    31201 aacttaatcg attatgatca atccctgttg ccattcgtct cggattgtta aaagtcgttg
    31261 gagttaccag cgcttgaact tctattaaaa gtggtctggt tccctccatg gttgcaacaa
    31321 ttgttgaacc tggaacattt gttgaacgtt cttctaaaaa catttcagat ggattattta
    31381 caccttttaa tccactttgc ttcatttcga agattcccat ttcattagtt gaaccaaaac
    31441 ggtttttaac agctcgcaaa attcgatatg cgtggtgttc atcgccttca aaataaagca
    31501 cagtatcaac catgtgttct agcaatcttg ggccagcaat ttgaccttct ttcgttacat
    31561 gacccactat aaaagttgca atgttcattt gtttagcaat attcattaaa ctttgtgtac
    31621 tttcacgaac ttgtgaaaca gaacctggcg cagagctgat ttcaggatga tatattgttt
    31681 gaatcgaatc cactactaat aaatcaggtt gttcttcttt tactgtttga taaataactt
    31741 caagatctgt ttcagctaat acttgcaatt cacttgaatc ttcatctaat cgctctgcac
    31801 gtaatttagt ctgactaagc gattcttctc cagtaatata tagtactttt ttcttttgag
    31861 ataacgatgc acaaatttgt aaaagtaacg ttgacttacc aatacctgga tccccaccaa
    31921 taagtactaa cgatccgctc acaatacctc cacctaatac acggttgaat tctgctgaat
    31981 ctgttaacac tctcggcgtt gtttcatgtt taatactatt taatttttgt actttacctg
    32041 ctaattcctt ggttttaact ccatgtttag gattggctgc tttttcaaca atttcctcca
    32101 tttgattcca agcgccacaa ttaggacatt tccccatcca tttaggagat tgataaccac
    32161 aagccataca ttcaaaaatc acttttttct tggccaaaat tgcacctcca ctttcctttg
    32221 aacaaatcta ttttaaacgc acattcccat tatgacaaat taaatgtgag ttacatttgt
    32281 ttgtatttta acatgactac taacgcaaac t
//
LOCUS       DAXWFK010000009        31959 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.009, whole genome shotgun sequence.
ACCESSION   DAXWFK010000009 DAXWFK010000000
VERSION     DAXWFK010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 31959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31959
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.009"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            47..280
                     /locus_tag="ACGEXS_000342"
     CDS             47..280
                     /locus_tag="ACGEXS_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFF8011390.1"
                     /translation="MDYIGQYAVIHLVLHVVCICIAYWALQSIRLDQFFKKGYATQLQ
                     VCMIFVAILLGTAVSNFIVDLLQYSTQVKYLIK"
     gene            391..1656
                     /gene="murA"
                     /locus_tag="ACGEXS_000343"
     CDS             391..1656
                     /gene="murA"
                     /locus_tag="ACGEXS_000343"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFF8011391.1"
                     /translation="MDKIVIKGGNKLTGEVKVEGAKNAVLPILTASLLASDKPSKLVN
                     VPALSDVETINNVLTTLNADVTYKKDENAVVVDATKTLNEEAPYEYVSKMRASILVMG
                     PLLARLGHAIVALPGGCAIGSRPIEQHIKGFEALGAEIHLENGNIYANAKDGLKGTSI
                     HLDFPSVGATQNIIMAASLAKGKTLIENAAKEPEIVDLANYINEMGGRITGAGTDTIT
                     INGVESLHGVEHAIIPDRIEAGTLLIAGAITRGDIFVRGAIKEHMASLVYKLEEMGVE
                     LDYQEDGIRVRAEGELQPVDIKTLPHPGFPTDMQSQMMALLLTANGHKVVTETVFENR
                     FMHVAEFKRMNANINVEGRSAKLEGKSQLQGAQVKATDLRAAAALILAGLVADGKTSV
                     TELTHLDRGYVDLHGKLKQLGADIERIND"
     gene            1690..2130
                     /gene="fabZ"
                     /locus_tag="ACGEXS_000344"
     CDS             1690..2130
                     /gene="fabZ"
                     /locus_tag="ACGEXS_000344"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFF8011392.1"
                     /translation="METIFDYNQIKQIIPHRQPFLLIDKVVEYEEGQRCVAIKQVSGN
                     EPFFQGHFPEYAVMPGVLITEALAQTGAVAILNSEENKGKIALFAGIDKCRFKRQVVP
                     GDTLTLEVEITKIKGPIGKGNAKATVDGQLACSCELTFAIQDVK"
     gene            complement(2189..2629)
                     /locus_tag="ACGEXS_000345"
     CDS             complement(2189..2629)
                     /locus_tag="ACGEXS_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwpF-like family protein"
                     /protein_id="HFF8011393.1"
                     /translation="MKTFKAVRFQIVNEHGRIIEYELEDGVIINKEESGTGWLLEIVI
                     SNEHYETFKEYQDNEQLLDIRVVITRPANDPALFESTVKSIKNFKTTMSIVFECHIYT
                     LRQQYAESLLEQLIDDGLSGEELKKSFNRMMQSKPKLKDEKLEE"
     gene            2823..3218
                     /locus_tag="ACGEXS_000346"
     CDS             2823..3218
                     /locus_tag="ACGEXS_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFF8011394.1"
                     /translation="MLNKIVIVGRLTKDAQIFEKEDRKIATFCVATHRNYKDENGEIV
                     CDYLFCKAFGKLASNIEKYTNQGTLVGITGQMRSRKYDKDGQTHFVTELYVETIKFMS
                     PKSQNNEILSDSILDIDSQNIDNHDLLEI"
     gene            3607..4302
                     /gene="sceD"
                     /locus_tag="ACGEXS_000347"
     CDS             3607..4302
                     /gene="sceD"
                     /locus_tag="ACGEXS_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase SceD"
                     /protein_id="HFF8011395.1"
                     /translation="MKKTLLASSLAVGLGIVAGNAGHEAHASEADLNKASLAQMAQSN
                     DQTLNQKPIEAGAYNYTFDYEGFTYHFESDGTHFAWNYHATGTNGADMSAQAPATNNV
                     APSAVQANQVQSQEVEAPQNAQTQQPQASTSNNSQVTATPTESKSSEGSSVNVNAHLK
                     QIAQRESGGNIHAVNPTSGAAGKYQFLQSTWDSVAPAKYKGVSPANAPESVQDAAAVK
                     LYNTGGAGHWVTA"
     regulatory      4506..4608
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4688..5377
                     /gene="tenA"
                     /locus_tag="ACGEXS_000348"
     CDS             4688..5377
                     /gene="tenA"
                     /locus_tag="ACGEXS_000348"
                     /EC_number="3.5.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050334 - thiaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HFF8011396.1"
                     /translation="MEFSQKLYQAAKPIINDIYEDDFIQKMLSGDIGADALRHYLKAD
                     AAYLKEFTNLYALLIPKMNSMNDVKFLVEQIEFMVEGEVLAHDILAQIVGESYEEIIK
                     TKVWPPSGDHYIKHMYFQAHSRENAIYTIAAMAPCPYIYAELAKRSQSDHKLNREKDT
                     AKWFDFYSTEMDDIINVFEALMNKLAESMSDKELEQVKQVFLESCIHERRFFNMAMTL
                     EQWEFGGKVND"
     gene            5370..6200
                     /gene="thiD"
                     /locus_tag="ACGEXS_000349"
     CDS             5370..6200
                     /gene="thiD"
                     /locus_tag="ACGEXS_000349"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFF8011397.1"
                     /translation="MIKPKIALTIAGTDPTGGAGVMADLKSFHSCGVYGMGVVTSIVA
                     QNTLGVQHIHNLNHQWVDEQLDSVFNDTLPHAIKTGMIATADTMETIRHYLMQHESIP
                     YVIDPVMLAKSGDSLMDNDTKQNLQHTLLPLADVVTPNLPEAEEITGLTIDSEEKIMQ
                     AGRIFINEIGSKGVIIKGGHSNDTDIAKDYLFTNEGVQTFENERFKTKHTHGTGCTFS
                     AVITAELAKGRPLFEAVHKAKKFISMSIQYTPEIGRGRGPVNHFAYLKKEGLDDELSK
                     "
     gene            6184..6975
                     /gene="thiM"
                     /locus_tag="ACGEXS_000350"
     CDS             6184..6975
                     /gene="thiM"
                     /locus_tag="ACGEXS_000350"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFF8011398.1"
                     /translation="MNYLNKIRIENPLTICYTNDVVKNFTANGLLSIGASPAMSEAPE
                     EAEEFYKVAQALLINIGTLTAENEQDIIAIAQTANEAGLPIVFDPVAVGASTYRKQFC
                     KLLLKSAKVSVIKGNASEILALIDDTATMKGTDSDANLDAVAIAKKAYATYKTAIVIT
                     GKEDVIVQDNKAFVLANGSPLLARVTGAGCLLGGVIAGFLFRETEPDIEALIEAVSVF
                     NIAAEVAAENENCGGPGTFSPLLLDTLYHLNETTYQQRIRIQEVE"
     gene            6977..7618
                     /gene="thiE"
                     /locus_tag="ACGEXS_000351"
     CDS             6977..7618
                     /gene="thiE"
                     /locus_tag="ACGEXS_000351"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFF8011399.1"
                     /translation="MFNQSYLNVYFICGTSDVPSHRTIHEVLEAALKAGITLFQFREK
                     GESALKGNDKLVLAKELQHLCHQYDVPFIVNDDVSLAKEINADGIHVGQDDAKVKEIA
                     QYFTDKIIGLSISDLDEYAKSDLTHVDYIGVGPIYPTPSKHDAHIPVGPEMIATFKEM
                     NPQLPIVAIGGINTNNVAPIVEAGANGISVISAISKSENIEKTVNRFKDFFNN"
     gene            7704..8576
                     /gene="yidC"
                     /locus_tag="ACGEXS_000352"
     CDS             7704..8576
                     /gene="yidC"
                     /locus_tag="ACGEXS_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFF8011400.1"
                     /translation="MKKKALLPLFLGIMVFLAGCDYSKPEKRSGFFYNTFVDPMKNVL
                     DWLGNNLLNDNYGLAIIILVLVIRIILLPFMLSNYKNSHMMRQKMKVAKPEVEKIQEK
                     VKRARTQEEKMAANQELMQVYKKYDMNPIKSMLGCLPMLIQLPIIMGLYFVLKDQLVD
                     GLFKYPHFLWFDLGRPDIWITIIAGVLYFIQAYVSSKTMPDEQRQMGYMMMVISPIMI
                     IWISLSSASALGLYWSVSAAFLVVQTHFANIYYEKVAKKEVQPFIEAYEREHNGGSNK
                     KGKNTQVVSKKKKK"
     gene            complement(9389..10036)
                     /locus_tag="ACGEXS_000353"
     CDS             complement(9389..10036)
                     /locus_tag="ACGEXS_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFF8011401.1"
                     /translation="MQQSDVISAAKKYMESIHQNDYTGHDIAHVYRVTALAKSIAENE
                     GVNDTLVIELACLLHDTVDEKVVDANKQYVELKSFLSSLSLSTEDQEHILFIINNMSY
                     RNGKNDHVTLSLEGQIVRDADRLDAIGAIGVARTFQFAGHFGEPMWTEHMSLDKINDD
                     LVEQLPPSAIKHFFEKLLKLESLMHTDTAKMIAKERHDFMMMYLKQFFTEWNCHD"
     gene            complement(10064..11548)
                     /gene="cls"
                     /locus_tag="ACGEXS_000354"
     CDS             complement(10064..11548)
                     /gene="cls"
                     /locus_tag="ACGEXS_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFF8011402.1"
                     /translation="MIELLSIALKHSNIILNSIFIGAFILNLLFAFTIIFMERRSANS
                     IWAWLLVLVFLPLFGFILYLLLGRQIQRDQIFKIDKEDKKGLELIVDEQLAALKNENF
                     SNSNYQIVKFKEMIQMLLYNNAAFLTTDNDLKIYTDGQEKFDDLIQDIRNATDYIHFQ
                     YYIIQNDELGRTILNELGKKAEQGVEVKILYDDMGSRGLRKKGLRPFRNKGGHAEAFF
                     PSKLPLINLRMNNRNHRKIVVIDGQIGYVGGFNVGDEYLGKSKKFGYWRDTHLRIVGD
                     AVNALQLRFILDWNSQATRDHISYDDRYFPDVNSGGTIGVQIASSGPDEEWEQIKYGY
                     LKMISSAKKSIYIQSPYFIPDQAFLDSIKIAALGGVDVNIMIPNKPDHPFVFWATLKN
                     AASLLDAGVKVFHYDNGFLHSKTLVIDDEIASVGTANMDHRSFTLNFEVNAFIYDQQI
                     AKKLKQAFIDDLAVSSELTKARYAKRSLWIKFKEGISQLLSPIL"
     gene            11714..12007
                     /gene="csoR"
                     /locus_tag="ACGEXS_000355"
     CDS             11714..12007
                     /gene="csoR"
                     /locus_tag="ACGEXS_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-sensing transcriptional repressor CsoR"
                     /protein_id="HFF8011403.1"
                     /translation="MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDV
                     LTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQEEAMEELLVTFQKLIKD"
     gene            12019..12228
                     /locus_tag="ACGEXS_000356"
     CDS             12019..12228
                     /locus_tag="ACGEXS_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HFF8011404.1"
                     /translation="MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVT
                     VSYETPANLNSIEKEIYDEGYKIVF"
     gene            12356..12493
                     /locus_tag="ACGEXS_000357"
     CDS             12356..12493
                     /locus_tag="ACGEXS_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lmo0850 family protein"
                     /protein_id="HFF8011405.1"
                     /translation="MKRPEKIQNVVKLLSSLGVNIKKTKSRLDIINTLPASNKVSHEL
                     K"
     gene            complement(12757..13959)
                     /locus_tag="ACGEXS_000358"
     CDS             complement(12757..13959)
                     /locus_tag="ACGEXS_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFF8011406.1"
                     /translation="MNYSSRQQPDKHWLRKVDWVLVATIAVLAIFSVLLINSAMGGGQ
                     YSANFGIRQIFYYILGAIFAGIIMFISPKKIKHYTYLLYFLICLLLIGLLVIPESPIT
                     PIINGAKSWYTFGPISIQPSEFMKIILILALARVVSRHNQFTFNKSFQSDLLLFFKII
                     GVSLVPSILILLQNDLGTTLVLAAIIAGVMLVSGITWRILAPIFITGIVGAMTVILGI
                     LYAPALIENLLGVQLYQMGRINSWLDPYTYSSGDGYHLTESLKAIGSGQLLGKGYNHG
                     EVYIPENHTDFIFSVIGEELGFIGSVILILIFLFLIFHLIRLAAKIEDQFNKIFIVGF
                     VTLLVFHILQNIGMTIQLLPITGIPLPFISYGGSALWSMMTGIGIVLSIYYHEPKRYV
                     DLYHPKSN"
     gene            14277..15347
                     /locus_tag="ACGEXS_000359"
     CDS             14277..15347
                     /locus_tag="ACGEXS_000359"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFF8011407.1"
                     /translation="MTKENICIVFGGKSAEHEVSILTAQNVLNAIDKDKYHVDIIYIT
                     NDGDWRKQNNITAEIKSTDELHLENGEALEISQLLKESSSGQPYDAVFPLLHGPNGED
                     GTIQGLFEVLDVPYVGNGVLSAASSMDKLVMKQLFEHRGLPQLPYISFLRSEYEKYEH
                     NILKLVNDKLNYPVFVKPANLGSSVGISKCNNEAELKEGIKEAFQFDRKLVIEQGVNA
                     REIEVAVLGNDYPEATWPGEVVKDVAFYDYKSKYKDGKVQLQIPADLDEDVQLTLRNM
                     ALEAFKATDCSGLVRADFFVTEDNQIYINETNAMPGFTAFSMYPKLWENMGLSYPELI
                     TKLIELAKERHQDKQKNKYKID"
     gene            15362..16720
                     /gene="murF"
                     /locus_tag="ACGEXS_000360"
     CDS             15362..16720
                     /gene="murF"
                     /locus_tag="ACGEXS_000360"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFF8011408.1"
                     /translation="MINVTLKQIQSWIPCEIEDQFLNQEINGVTIDSRAISKNMLFIP
                     FKGENVDGHRFVSKALQDGAGAAFYQRGTPIDENVSGPIIWVEDTLTALQQLAQAYLR
                     HVNPKVIAVTGSNGKTTTKDMIESVLHTEFKVKKTQGNYNNEIGLPLTILELDNDTEI
                     SILEMGMSGFHEIEFLSNLAQPDIAVITNIGESHMQDLGSREGIAKAKSEITIGLKDN
                     GTFIYDGDEPLLKPHVKEVENAKCISIGVATDNALVCSVDDRDTTGISFTINNKEHYD
                     LPILGKHNMKNATIAIAVGHELGLTYNTIYQNLKNVSLTGMRMEQHTLENDITVINDA
                     YNASPTSMRAAIDTLSTLTGRRILILGDVLELGENSKEMHIGVGNYLEEKHIDVLYTF
                     GNEAKYIYDSGQQHVEKAQHFNSKDDMIEVLINDLKAHDRVLVKGSRGMKLEEVVNAL
                     IS"
     gene            17237..18757
                     /locus_tag="ACGEXS_000361"
     CDS             17237..18757
                     /locus_tag="ACGEXS_000361"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFF8011409.1"
                     /translation="MQNFKELGISDNTVQSLESMGFKEPTPIQKDSIPYALQGIDILG
                     QAQTGTGKTGAFGIPLIEKVVGKQGVQSLILAPTRELAMQVAEQLREFSRGQGVQVVT
                     VFGGMPIERQIKALKKGPQIVVGTPGRVIDHLNRRTLKTDGIHTLILDEADEMMNMGF
                     IDDMRFIMDKIPAVQRQTMLFSATMPKAIQALVQQFMKSPKIIKTMNNEMSDPQIEEF
                     YTIVKELEKFDTFTNFLDVHQPELAIVFGRTKRRVDELTSALISKGYKAEGLHGDITQ
                     AKRLEVLKKFKNDQINILVATDVAARGLDISGVSHVYNFDIPQDTESYTHRIGRTGRA
                     GKEGIAVTFVNPIEMDYIRQIEDANGRKMSALRPPHRKEVLQAREDDIKEKVENWMSK
                     ESESRLKRISTELLNEYNDVDLVAALLQELVEANDEVEVQLTFEKPLSRKGRNGKPSG
                     SRNRNSKRGNPKFDSKSKRSKGYSSKKKSTKKFDRKEKSSGGSRPMKGRTFADHQK"
     gene            complement(19126..19821)
                     /locus_tag="ACGEXS_000362"
     CDS             complement(19126..19821)
                     /locus_tag="ACGEXS_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFF8011410.1"
                     /translation="MQSKILIIEDDHAITHLLDVALTLDYYNVTTADNATQAHFKIQI
                     DKPDVILLDLGLPDKDGLCLISEIRQHTDIPIIVISARQEEQTIIQALDNGANDYMTK
                     PFNVDELRARIRVIERIAKSHQETNIVFTNGLLSIDFGSKSVVINNQEVHLTPNEFSL
                     LELLSNHKGKVLTYEMILKRIYGYVNKTEMPSLRVHMTSLRQKLSQCHEDAKDIIKTH
                     PRIGYQMLQWKEK"
     gene            complement(19821..22478)
                     /locus_tag="ACGEXS_000363"
     CDS             complement(19821..22478)
                     /locus_tag="ACGEXS_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HFF8011411.1"
                     /translation="MSNTESLNIGKKRGSLTIYIGYSPGVGKTFEMLSNAIELFQSNV
                     DIKIGYIEPHQRDETNALAEQLPKITTNFTKHGSHHFQYLDVDRIIEESPTIVLIDEL
                     AHTNISRDRHEKRYMDIEEILNHGIDVHTTLNIQHIESLSSQIELMTGVHVKERVPDY
                     FIMSADVLEVVDISPEQLIKRLKAGKVYKKDRLDVAFSNFFTYAHLSELRTLTLRTVA
                     DLMSDKEKVRHNHKTSLKPHIAVAISGSIYNEAVIKEAFHIAQKEHAKFTAIYIDVFE
                     KNRQYKDSQKQVHQHLMLAKSLGAKVKVVYSQTVALGLDEWCKNQDVTKLIIGQHIRN
                     KWRDFFNTPLIDHLMSFEHSYKIEIVPIKQIPVELKMNKSPYRPKGKRFAIDMLKMIL
                     IQIICVMMGLWIYQLDKHESSTIILMIFLIGIILLSIWTRSFIIGFLAAIINVFVFNY
                     FFTEPRYTFEVYRFDYPITFIVSILTSILTSALLKQIKFQYSITKKQLYRTDLLFQFN
                     DSIKQTYTVENLLINAGYQINQLLQQSITIYVINQSKVIKTIPLQNHIDNTTQQHEQA
                     LSWVIKNERQAGATTDTFPGINKWLIPIGTSPIKGILAIDYQSSQVINPYDASILESM
                     LNELSLAVENVTLLKQTRESMLQAERQLTHSNFLRSISHDIRTPLTTIMGNLDILVSH
                     SKDMSIIEKEQLLVHSFQESQYLYLLVTNILSLTKLQSSNVQIKLQPYLVSELVEEID
                     MILERRHLKKRITVSSSVNLQFIHIDSKLILQALFNLIENAVKHTSTDTKINLSIRYA
                     SYEQIEFAVIDEGPGISLEEQQKIFEPFYTGSNKYFKDNQKESMGLGLYLVQTILHKH
                     QSNLQYKPNQPHGSIFYFNIYTDFNEGDV"
     gene            22616..22726
                     /gene="kdpF"
                     /locus_tag="ACGEXS_000364"
     CDS             22616..22726
                     /gene="kdpF"
                     /locus_tag="ACGEXS_000364"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0043462 - regulation of ATP-dependent
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFF8011412.1"
                     /translation="MLLCCKEVMAMLIVLLLVVIALVLYLFYALIRSEKF"
     gene            22750..24426
                     /gene="kdpA"
                     /locus_tag="ACGEXS_000365"
     CDS             22750..24426
                     /gene="kdpA"
                     /locus_tag="ACGEXS_000365"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFF8011413.1"
                     /translation="MEIILFLTMMVMITYVFSGYLYRVALVQSSRVDLIFTRFENMCF
                     KIIGTDLEHMSAKTYVKHFLAFNGFMGFITFVLLIVQQWLFLNPNHNLNQSIDLAFNT
                     AISFLTNSNLQHYNGESDVTYLTQMIVMTYLMFTSSASGYAVCIAMLRRLTGLTNIIG
                     NFYQDIVRFIVRVLLPLSCLISILLMTQGVPQTLHANLMIRTLSGHIQHIAFGPIASL
                     ESIKHLGTNGGGFLAGNSATPFENPNIWSNFIEMGSMMLLPMSMLFLFGRMLSRHGKR
                     VHRHALILFVAMFFIFIAILTLTMWSEYRGNPILANLGIYGPNMEGKEVRFGAGLSAL
                     FTVITTAFTTGSVNNMHDSLTPIGGLGPMVLMMLNVVFGGEGVGLMNLLIFVLLTVFI
                     CSLMVGKTPEYLNMPIGAREMKCIVLVFLIHPILILVFSALAFMIPGASESITNPSFH
                     GISQVMYEMTSAAANNGSGFEGLKDDTTFWNISTGIIMLLSRYIPIILQLMIASSLVN
                     KKSYHQDKYTIAIDKPYFGVSLIVFIVLLSGLTFIPVLLLGPIGEFLTLK"
     gene            24445..26472
                     /gene="kdpB"
                     /locus_tag="ACGEXS_000366"
     CDS             24445..26472
                     /gene="kdpB"
                     /locus_tag="ACGEXS_000366"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFF8011414.1"
                     /translation="MHHVNKYFNQTMVIEALKMSFYKLNLKQLIKNPIMFVVEVGMIL
                     TLILICFPDIFGTSYLSRGYLITIFIILLITILFANFSEAFAEGRGKAQADSLRQAQS
                     NLTARLIEENGAYRIVNATELKAGQNIRVENGETIPADGVVINGLATVDESAITGESA
                     PVIKESGGDFDGVIGGTLVTSDWLEIRVESEAGTSFLDKMIALVEGAERNKTPNEIAL
                     FTLLTTLTIIFLVVIVTLYPIASYLHLILPIAMLIALTVCLIPTTIGGLLSAIGIAGM
                     DRVTQFNVLAKSGRAVEVCGDVDVMILDKTGTITYGNRIASEFLPVNQQMLEKLIVAA
                     YMSSIYDDTPEGKSIVRLAKQMYINELPKDIDGTYKPFTAETRMSGIITNEISVFKGA
                     PNSMINLVKQQQGNIPLNIESLCMDVSSKGGTPLIVIENNVMLGVIYLKDVIKDGLVE
                     RFTELRKMGIETVMCTGDNALTAATIAKEAGVDRFVAECKPEDKIKVIKDEQAKGHIV
                     AMTGDGTNDAPALAQANIGLAMNSGTISAKEAANLIDLDSNPTKLIEVVKIGKQLLMT
                     RGALTTFSLANDVAKYFAILPALMMSTIPEMTSLNIMHLSSPKSAIISALIFNALIIV
                     ALIPIAMKGVKVKGYSIDRIFINNMLIYGLGGLIVPFLGIKLIDMIVQFFV"
     gene            26492..27052
                     /locus_tag="ACGEXS_000367"
     CDS             26492..27052
                     /locus_tag="ACGEXS_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFF8011415.1"
                     /translation="MNTIRNSICLTIITMVLCGFLFPLAITLIGQIFFYQQANGSLIT
                     YDNRIVGSKLIGQHWTETRYFHGRPSAVDYNMNPEKLYKNGVSSGGSNESNGNTELIA
                     RMKHHVKFGNSNVTIDAATSSGSGLDPHITVENALKQAPRIADARHVSTSRVADLIQH
                     RKQRGVLTNDYVNVLELNIALDKMKD"
     gene            27261..27740
                     /locus_tag="ACGEXS_000368"
     CDS             27261..27740
                     /locus_tag="ACGEXS_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HFF8011416.1"
                     /translation="MSDYTKLTKSPKSALLYYYITNGLEFILSVAVYVIFYFIWLRFE
                     WPHYLLYILLVLGALSTLKLFIKPLWQYHCRFYKVDQLSVQYRTSFFIYKEETSRIER
                     LQYLSIKSNPISKVLNLYKVGFMTAGHTIYLPMMSHDDVKIIEERTMSNLRGVESDV"
     gene            27733..29316
                     /locus_tag="ACGEXS_000369"
     CDS             27733..29316
                     /locus_tag="ACGEXS_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HFF8011417.1"
                     /translation="MSEPQKLHPISYFNGIINAIKQNIVVFFIFIIFQLKDFDYTNPE
                     SYLWIGIVFVFFLISYISQIVGIMNTRYWIEDNYFILTTGIFNKKRKELNIKRIQSVD
                     MTQGVVNQIIGGVDLQIKTPSDGIVLSVISKKQGEYLERYIDQLQTELKTEVAQKSSA
                     EKNDNCNHDIEGNVEDIAEGRLDEVQNTDSMLNQKNKRIPIYQMNFKESLFMAMTSGA
                     IGVTLAALVPIYGAVRELIPWSKLNHEISQWVQIVSISVLIIILLVLIVAYIIGTIIT
                     MIRFYGFSVMLENEQLKIEYGLFNKKKVTVPTKRLQAVVERQSYIRRLFGYTAIHFII
                     TSDFENKKIEDDDNDSGSVPILPFIKRDKAYQIIQNLVPDLSYQQVNEGMPLSGFHRH
                     FLIPSIIVIAMAGIGCYFWNQLLLLFTIITIVIIGLFVIKGYLYVKHAGFKMTEEQIT
                     IQNFELLKRNIYYFKAHHIIGFDTWQHTLLKRNNLNNFSFIVAKGVGAQSINLKYARS
                     EDANKLKSWYLRGENDEGL"
     gene            29303..29794
                     /locus_tag="ACGEXS_000370"
     CDS             29303..29794
                     /locus_tag="ACGEXS_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HFF8011418.1"
                     /translation="MRDYNFMSPHAKKVMRLSAVLFWIPIALVLLIAFNILNWLFWEI
                     LDNHLSIVSSVIIILLVAVFTLVIAPVYRYKHCRYAFEKHHLRVRNGILFLDEKVIPY
                     FRIQNIDIDVGPIMRRYRLATLTLYTAGGQAEIELIDRSEARRLKRWLNNERHLENNK
                     NEE"
     gene            29798..30157
                     /gene="acpS"
                     /locus_tag="ACGEXS_000371"
     CDS             29798..30157
                     /gene="acpS"
                     /locus_tag="ACGEXS_000371"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFF8011419.1"
                     /translation="MIHGIGVDLIEIDRIQALYSKQPKLVERILTKNEQHKFNNFTHE
                     QRKIEFLAGRFATKEAFSKALGTGLGKHVAFNDIDCYNDELGKPKIDYEGFIVHVSIS
                     HTEHYAMSQVVLEKSAF"
     gene            30223..31371
                     /gene="alr"
                     /locus_tag="ACGEXS_000372"
     CDS             30223..31371
                     /gene="alr"
                     /locus_tag="ACGEXS_000372"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFF8011420.1"
                     /translation="MSDKYYRSAYMNVDLNAVASNFKVFSTLHPNKTVMAVVKANAYG
                     LGSVKVARHLMENGATFFAVATLDEAIELRMHGITAKILVLGVLPAKDIDKAIQHRVA
                     LTVPSKQWLKEAIKNISGEQEKKLWLHIKLDTGMGRLGIKDTKTYQEVIEIIQQYEQL
                     VFEGVFTHFACADEPGDMTTEQYQRFKDMVNEAIKPEYIHCQNSAGSLLMDCQFCNAI
                     RPGISLYGYYPSEYVQQKVKVHLKPSVQLIANVVQTKTLQAGESVSYGATYTATDSTT
                     IALLPIGYADGYLRIMQGSFVNVNGHQCEVIGRVCMDQTIVKVPDQVKAGDSVILIDN
                     HRESPQSVEVVAEKQHTINYEVLCNLSRRLPRIYHDGDQRFVTNELLK"
     gene            31456..31626
                     /gene="mazE"
                     /locus_tag="ACGEXS_000373"
     CDS             31456..31626
                     /gene="mazE"
                     /locus_tag="ACGEXS_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFF8011421.1"
                     /translation="MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDC
                     NETYLSSNSTNE"
     gene            31623..>31959
                     /locus_tag="ACGEXS_000374"
     CDS             31623..>31959
                     /locus_tag="ACGEXS_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFF8011422.1"
                     /translation="MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVA
                     AITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIRTLDKKRLKEKLTYLSDDKMKEV
                     DNALMISLGL"
BASE COUNT        11135 a         4737 c         5840 g        10247 t
ORIGIN      
        1 aatttttcat gtacaatgta atacagtaat cttatgaggt gataaaatgg attatatcgg
       61 acaatatgca gttatccatt tagtgttaca tgttgtatgt atttgtattg cctattgggc
      121 tttacaatca attagattag atcaattttt taaaaaagga tacgccactc aattacaagt
      181 gtgtatgata tttgttgcta ttttattagg cactgcagta agcaatttta ttgtagattt
      241 gttacaatac tcgacgcagg taaaatattt aataaaataa gtctaactct atgatttgta
      301 atcaaaacta gatataatta aataatgact taaaataatt ttaaaatagg gaaatgtaaa
      361 gtaataggag ttctaagtgg aggatttacg atggataaaa tagtaatcaa aggtggaaat
      421 aaattaacgg gtgaagttaa agtagaaggt gctaaaaatg cagtattacc aatattgaca
      481 gcatctttat tagcttctga taaaccgagc aaattagtta atgttccagc tttaagtgat
      541 gtagaaacaa taaataatgt attaacaact ttaaatgctg acgttacata caaaaaggac
      601 gaaaatgctg ttgtcgttga tgcaacaaag actctaaatg aagaggcacc atatgaatat
      661 gttagtaaaa tgcgtgcaag tattttagtt atgggacctc ttttagcaag actaggacat
      721 gctattgttg cattgcctgg tggttgtgca attggaagta gaccgattga gcaacacatt
      781 aaaggttttg aagctttagg cgcagaaatt catcttgaaa atggtaatat ttatgctaat
      841 gctaaagatg gattaaaagg tacatcaatt catttagatt ttccaagtgt aggagcaaca
      901 caaaatatta ttatggcagc atcattagct aagggtaaga ctttaattga aaatgcagct
      961 aaagaacctg aaattgtcga tttagcaaac tacattaatg aaatgggtgg tagaattact
     1021 ggtgctggta cagacacaat tacaatcaat ggtgtagaat cattacatgg tgtagaacat
     1081 gctatcattc cagatagaat tgaagcaggc acattactaa tcgctggtgc tataacgcgt
     1141 ggtgatattt ttgtacgtgg tgcaatcaaa gaacatatgg cgagtttagt ctataaacta
     1201 gaagaaatgg gcgttgaatt ggactatcaa gaagatggta ttcgtgtacg tgctgaaggg
     1261 gaattacaac ctgtagacat caaaactcta ccacatcctg gattcccgac tgatatgcaa
     1321 tcacaaatga tggcattgtt attaacggca aatggtcata aagtcgtaac cgaaactgtt
     1381 tttgaaaacc gttttatgca tgttgcagag ttcaaacgta tgaatgctaa tatcaatgta
     1441 gaaggtcgta gtgctaaact tgaaggtaaa agtcaattgc aaggtgcaca agttaaagcg
     1501 actgatttaa gagcagcagc cgccttaatt ttagctggat tagttgctga tggtaaaaca
     1561 agcgttactg aattaacgca cctagataga ggctatgttg acttacacgg taaattgaag
     1621 caattaggtg cagacattga acgtattaac gattaattca gtaaattaat ataatggagg
     1681 atttcaacca tggaaacaat ttttgattat aaccaaatta aacaaattat acctcacaga
     1741 cagccatttt tattaattga taaagtagtt gaatatgaag aaggtcaacg ttgtgtggct
     1801 attaaacaag tatcaggaaa cgaaccattc tttcaagggc attttcctga gtatgcggta
     1861 atgccaggcg tattaattac tgaagcgtta gctcaaacag gtgcggtagc tattttaaat
     1921 agtgaagaaa ataaaggtaa aatcgcttta tttgctggta ttgataaatg tcgttttaaa
     1981 cgtcaagtag tacctggtga tactttaacg ttggaagtag aaatcactaa aattaaagga
     2041 ccaataggta aaggtaatgc taaagctact gtcgatggtc aacttgcttg tagttgtgaa
     2101 cttacatttg caattcaaga tgtaaaataa aacaaaaaaa acattcaaag atttaatgtg
     2161 ttggcataat ctttgaatgt tttttatttt actcttctaa tttttcatcc tttaactttg
     2221 gtttagactg catcattcga ttaaatgatt tttttaattc ttcaccagat aatccatcat
     2281 caataagttg ttctaataaa ctttcagcat actgttgacg taaagtgtaa atatgacatt
     2341 caaatacgat agacattgtt gttttaaaat ttttaatact tttcacagta gattcaaata
     2401 aagctggatc attggctgga cgcgtgatga cgacacgaat atccaataac tgttcattgt
     2461 cttgatattc cttaaatgtt tcataatgct cattagaaat gacaatttca agcaaccaac
     2521 ccgtgccact ttcctcttta ttaattatta cgccatcttc taattcatat tctataattc
     2581 tgccgtgttc attcacaatt tgaaacctta ctgctttaaa agttttcata ttcccacatc
     2641 ccattagttt aatatttatg atttttgaat tatttagaag atttaaggtc atttcatgac
     2701 taatttcttt aaatataaca aataacgaga tgctatagct attaattata taaaatttgc
     2761 cattttgacc atttttaagc tagtaattaa gatataggca taggaggtga atcacacact
     2821 acatgctaaa taaaatcgta attgtcggga gactgacgaa agacgcacaa atatttgaaa
     2881 aggaggatag aaaaattgca acgttttgtg ttgcaacgca ccgaaattat aaagatgaaa
     2941 atggagaaat cgtctgtgat tacttattct gtaaagcatt tggcaagtta gcttctaata
     3001 tagaaaaata tactaatcaa ggtacattgg ttggtataac tggtcaaatg agatcaagaa
     3061 agtatgataa agacggacaa acacactttg tcactgaatt atatgttgaa acaataaaat
     3121 ttatgtcccc taaatcccaa aataatgaaa ttctctcaga tagtatttta gatattgact
     3181 ctcaaaatat agataatcat gacttattag aaatttaaat ccaattatcc taaacatcct
     3241 taatatacat ttaatccatc ctaaattttt tatagatcta attaacttga aatatactca
     3301 cttaaaaacg acttacatga ctagtactgt tgtgtaggtc gtctttttgt gtagtttttc
     3361 taatagaaaa tagacataga ttgcaaaacg attacataaa aaattgcaaa taaaaaacat
     3421 ttttactgtc atgacaattc tcgatagaat catatctgaa attatggggg atttcgttgt
     3481 aatcactgtg taaaaaacaa ggctattttt ttattttgtt gtaacctgca cggattttat
     3541 agatgttaca gtagtctctg taaaactttt agagaaacaa attacttgta ggaggatttt
     3601 aagattatga agaaaacatt actcgcatca tcattagcag taggtttagg aatcgtagca
     3661 ggaaatgcag gtcacgaagc ccatgcaagt gaagcggact taaataaagc atctttagcg
     3721 caaatggcgc aatcaaatga tcaaacatta aatcaaaaac caattgaagc tggggcttat
     3781 aattatacat ttgactatga agggtttact tatcactttg aatcagatgg tacacacttt
     3841 gcttggaatt accatgcaac aggtactaat ggagcagaca tgagtgcaca agcacctgca
     3901 actaataatg ttgcaccatc agctgttcaa gctaatcaag tacaatcaca agaagttgaa
     3961 gcaccacaaa atgctcaaac tcaacaacca caagcatcaa catcaaacaa ttcacaagtt
     4021 actgcaacac caactgaatc aaaatcatca gaaggttcat cagtaaatgt gaatgctcat
     4081 ctaaaacaaa ttgctcaacg tgaatcaggt ggcaatattc atgctgtaaa tccaacatca
     4141 ggtgcagctg gtaagtatca attcttacaa tcaacttggg attcagtagc acctgctaaa
     4201 tataaaggtg tatcaccagc aaatgctcct gaaagtgttc aagatgccgc agcagtaaaa
     4261 ttatataaca ctggtggcgc tggacattgg gttactgcat aagccattta tgcatagcta
     4321 attaatagtt atataagtaa cttttagatt ggaatatatc ggggaattct cgatatgttc
     4381 cggttttttt atttcgcaaa tatatgtcat tatagaatta ttatttaaag ataataatac
     4441 aactgagcaa caataattga aaatgttgaa accgtttgct aagattaatt tgcaattgaa
     4501 tactaaggaa ctactagggg agcctaatga tatggctgag atgaattgtt cagaccctta
     4561 tgacctgatt tggttagtac caacgtagga aagtagttat tgaagtgatg gggctagtat
     4621 agcaacattg ccatcaaact actttcatgt tggaagtagt ttttttattt agggaggatt
     4681 ttttacaatg gaattttcac aaaaattgta ccaagctgca aagccaatca ttaatgatat
     4741 ttatgaggat gactttattc agaagatgtt atccggagat ataggagctg acgcattacg
     4801 ccattactta aaagctgatg ctgcgtattt aaaagaattt acaaacttat atgctttgtt
     4861 aattccaaaa atgaatagca tgaatgacgt aaaattttta gtagagcaaa tagaatttat
     4921 ggttgaaggt gaagtacttg cgcatgatat tttagcgcaa attgttggtg aatcatacga
     4981 agaaatcatt aaaacaaaag tttggcctcc aagtggcgac cattacataa aacatatgta
     5041 tttccaagcg catagtcgtg aaaatgctat ttatacaatt gctgcaatgg caccttgtcc
     5101 atatatttat gcagaattag ctaagcgctc tcaaagtgat cataagctta atagagaaaa
     5161 agatacggca aaatggtttg atttttatag tactgaaatg gatgacataa ttaacgtatt
     5221 tgaggcatta atgaataaat tagccgaatc aatgtctgac aaggaattgg aacaagtaaa
     5281 gcaagtattt ttagaaagtt gcatacatga gcgtcgattt ttcaatatgg ctatgacact
     5341 agaacaatgg gaatttggag gaaaagtaaa tgattaaacc taaaatagca ttaaccattg
     5401 caggtactga tccaacaggt ggtgccggcg taatggctga tttaaaatca tttcattcat
     5461 gtggtgtata tggtatgggc gtcgttacaa gtattgttgc tcaaaataca ttgggcgtac
     5521 aacatattca taatttaaat catcaatggg tagatgaaca acttgatagt gtcttcaatg
     5581 ataccttacc tcatgctatt aaaacgggga tgattgctac agcagatact atggaaacga
     5641 ttcgtcatta tttaatgcaa catgaatcta ttccatatgt aattgatcct gttatgttgg
     5701 cgaaaagcgg tgattcatta atggataatg acacaaagca aaacttgcag catacgttat
     5761 tgccattagc tgacgtagta acaccgaatt taccagaagc tgaagaaata acgggactaa
     5821 ccattgatag tgaagaaaaa attatgcagg ctggccgcat ctttattaat gagattggta
     5881 gtaaaggtgt catcattaaa ggcggtcatt caaatgatac tgatatagca aaagattatt
     5941 tatttactaa cgaaggtgtt caaacatttg aaaatgaacg atttaaaaca aaacatacgc
     6001 atggaacagg gtgtacattt tcagcagtta taacggcaga acttgcaaaa ggtagaccat
     6061 tatttgaggc tgtacacaag gctaaaaagt ttatttcaat gagtatacaa tatacgcctg
     6121 aaatcggccg tggtagaggt ccagtgaatc attttgcata tttaaagaaa gagggattag
     6181 acgatgaatt atctaaataa aatacgtatt gaaaacccat taacaatttg ttatacaaac
     6241 gatgtagtta aaaattttac agcgaatggt ttattaagta ttggtgctag ccctgcaatg
     6301 agtgaagctc ccgaagaagc tgaagaattt tacaaagttg cacaagcgct attaatcaat
     6361 atcggtactt taacagcaga aaatgaacaa gatattattg cgattgctca aacggcaaat
     6421 gaggcaggct tacctattgt atttgaccct gtagctgttg gtgcttctac atatcgaaag
     6481 caattttgta aattattatt gaaatcagcg aaagtatcag taattaaagg caatgcatct
     6541 gaaatattag cgttgattga tgatacagca actatgaaag gtacagatag tgatgctaat
     6601 cttgatgcgg ttgcaatagc gaaaaaggct tacgcaacat ataaaactgc aatagtaatc
     6661 acaggtaaag aggacgttat tgttcaagat aataaagcct tcgtattagc taatggatct
     6721 ccattattag cacgagtaac tggagctggt tgtttattag gaggcgttat tgctggattt
     6781 ttatttagag aaacagaacc agacatagaa gcgttaattg aagcggtaag cgtatttaat
     6841 attgctgctg aggtagctgc tgaaaatgaa aattgtggtg gtcctggtac gttttcacca
     6901 ttgttgcttg atacgttata tcatttaaat gaaacaacct atcaacaacg gattcgtatt
     6961 caagaggtgg aataatatgt ttaaccaatc gtatctaaat gtgtatttta tttgtgggac
     7021 ctccgatgtt ccgagtcatc gaactattca tgaagtatta gaagcagcat taaaggcagg
     7081 gattacactt ttccaatttc gagaaaaggg tgaatcagct ttaaaaggaa acgataaact
     7141 tgtattagct aaagaattac agcatctctg tcatcaatat gatgtgcctt ttatcgttaa
     7201 tgatgatgta tccttggcaa aagaaattaa cgcggatggt atacatgtcg gtcaagatga
     7261 tgcgaaagtc aaagagatag cacaatattt cactgataaa attattggac ttagtattag
     7321 tgatttagat gagtatgcaa aatctgattt aactcatgtt gattatatag gtgttggacc
     7381 aatctatcct acgccgtcga agcatgacgc gcatatacca gtaggtccag aaatgattgc
     7441 aacgtttaaa gaaatgaatc cgcaactacc gattgtagct attggcggta tcaatactaa
     7501 taatgtggcg ccgattgtag aagcaggtgc caatggaatt tcagtcattt cagccatttc
     7561 taaaagtgaa aatattgaaa agactgttaa tcgattcaaa gattttttta ataattagtt
     7621 aaatttttcc acttattgtt tcatacgtac tcattaatgt gatagaataa atcctatgtg
     7681 aatataatga tagaggtgga aaaatgaaga aaaaagcgtt actaccatta tttttaggta
     7741 ttatggtctt tttggctggt tgtgactatt ctaaacctga aaaacgtagt gggtttttct
     7801 acaatacatt cgtagatcca atgaaaaatg tattggattg gttgggaaat aacttattaa
     7861 acgacaatta tggtttagct attattatcc ttgtattggt aattcgtatt attttattac
     7921 cattcatgtt gtcaaactat aaaaatagtc atatgatgcg tcaaaaaatg aaagttgcaa
     7981 agccagaagt tgaaaaaatt caagaaaaag tgaaacgtgc gcgtacacaa gaagaaaaaa
     8041 tggctgcaaa ccaagaatta atgcaagtat ataaaaagta tgacatgaac ccgattaaga
     8101 gtatgttggg ttgtttacca atgctaatcc aattaccaat catcatggga ttatactttg
     8161 tacttaaaga ccaacttgta gatggtttgt ttaaatatcc acacttctta tggttcgatt
     8221 taggacgtcc tgatatttgg attacaatta ttgccggtgt tttatacttt atccaagcat
     8281 atgtatcaag taaaacgatg ccagacgaac aacgtcaaat gggttacatg atgatggtca
     8341 tttcaccaat tatgattatc tggatttcat taagctcagc atcagcactt ggtttgtact
     8401 ggtcagtcag tgcggcgttc cttgtagttc aaacacactt tgcgaacatt tattatgaaa
     8461 aagtcgctaa aaaagaagta caacctttca ttgaagcgta tgaaagagag cacaacggcg
     8521 gcagcaataa aaaaggcaaa aacacacaag ttgtgtctaa aaagaaaaag aaataacctt
     8581 agcaagtcca aataatgtgc accctaaaag ttgaagataa ttttaacttt atagggcgca
     8641 cattattttt cgcttcaata gcgaaactta gtgtcacatt atacaatatg tgataaatag
     8701 aaacagcagt aagatgattt caaatgtgaa agtcatccta ctgctgtttt tatttgaaat
     8761 aatattaacg agttttcagt atgtaaaaag tggttaataa cagagttgtc attcttcatc
     8821 gcaatgtcat cttgacacaa tgattcatca gtgtagcagt atatgtcgca aatttccaca
     8881 gcaattcact tatacatgta acagtgtgaa ataataacgt cacaacggca taatacacat
     8941 tgcaacgtcc caacaacata tcatagaatc gtaacggcat gacatcaaca ccaaacctgg
     9001 ctataacacc ataacagcgc aacgccacat catagcacta catcaagtcg catcgtagca
     9061 ccagcacatc aaagtaacat ggcatcaaca tcaaaccggt ctataacacc ataacaccgc
     9121 aatgccacat catagcacta catcaagtcg catcgtggta ccagcacatc aaagtaacat
     9181 ggcatcaacg ccaaacctgt ctataacacc ataacaccgc aatgccacat catagcacta
     9241 catcaagacg catcgtggta ccagcacatc aaagtagcat gacatgtcac caagctccca
     9301 caacaacaca tcaaagtaac atggcatgac accaagcttc cacaacaaca cgccattaca
     9361 tcgcatcatc atactacaac ttcaatgtct agtcgtgaca attccattcc gtaaaaaact
     9421 gtttcaagta catcatcata aagtcgtgac gttctttagc aatcatcttc gccgtatctg
     9481 tatgcattaa agattctaac ttaagtaatt tttcaaagaa atgtttaatt gcagatggtg
     9541 gcaactgttc aactaaatca tcattaatct tatctagtga catatgttct gtccacatag
     9601 gttcaccaaa gtgtcctgca aattgaaatg ttcgtgcaac acctatagcg cctatagcat
     9661 caagacgatc tgcatccctg acaatttgac cttctaaaga taaagtgaca tgatcatttt
     9721 tgccattgcg atagctcata ttattaataa taaataaaat gtgctcttga tcttcggttg
     9781 atagtgataa agaagataaa aatgacttca attcaacata ttgtttgtta gcatctacaa
     9841 ctttttcgtc aacggtatca tgaagcaaac atgcgagttc aatgactaaa gtatcattaa
     9901 caccttcatt ttcagcgatt gatttagcta aagcagtgac acgatataca tgcgcaatat
     9961 catggcctgt ataatcattt tgatgaatag attccatata ttttttggca gcactaatga
    10021 cgtctgattg ttgcattaaa gttacactcc tcatatttct attttataag ataggtgaca
    10081 ataattgtga aataccttct ttaaatttaa tccaaagact tcgcttagca taacgtgctt
    10141 ttgttaattc agaagatact gctaaatcat ctataaaagc ttgttttaat tttttggcaa
    10201 tttgttggtc ataaataaaa gcgttgactt cgaaattcaa tgtgaaactg cgatggtcca
    10261 tattagctgt tcccacactt gcaatttcat catctataac aagtgttttt gagtgtaaaa
    10321 agccattgtc gtagtgaaat actttaacac cggcatctaa taaggatgct gcatttttta
    10381 aagtagccca aaaaacaaac ggatggtcag gtttattagg aatcatgata ttgacatcaa
    10441 caccacctaa tgccgcaatt ttaatagaat ctaaaaaggc ttgatcaggt atgaaatagg
    10501 gagattgaat ataaatcgat tttttagcag atgaaatcat tttcaaatag ccgtatttaa
    10561 tctgttccca ttcttcgtca ggaccactag aagctatttg aacgccaatt gttccaccag
    10621 aatttacatc tgggaaataa cgatcatcat aggagatgtg gtcacgtgtg gcttgtgaat
    10681 tccaatctag aataaatcgt aattgcaatg cattcactgc atccccgaca attcgtaaat
    10741 gcgtatctcg ccaatagccg aatttttttg atttacctaa gtactcatca ccaacattaa
    10801 aaccaccaac atatccaatt tgcccatcta ttacaacaat ttttcgatgg tttcgattgt
    10861 tcatacgcaa gttaattaaa ggtaattttg atgggaaaaa tgcttcagca tgtccacctt
    10921 tattgcgaaa cgggcgtaag ccttttttac gcagtccacg agaacccatg tcatcataaa
    10981 gaattttaac ttctacacct tgttccgctt ttttaccaag ttcatttaaa atggtacgac
    11041 ctaattcatc attttgaata atatagtact gaaaatgaat ataatcagta gcattacgga
    11101 tgtcttgtat taggtcatca aatttttctt ggccgtctgt gtatattttt aaatcgttgt
    11161 ctgttgttaa aaatgctgcg ttattatata acaacatttg aatcatttct ttaaatttta
    11221 caatttgata attggaattt gaaaagtttt cattttttaa agcagctaat tgctcatcaa
    11281 cgattaactc taatcctttt ttatcttcct tatcaatttt gaaaatttgg tcacgttgaa
    11341 tttgtcgtcc taatagtaag tataaaatga agccgaataa aggcaagaaa actaagacta
    11401 gtaaccaagc ccagatagaa ttggcagaac gtctttccat gaaaataatg gtaaaggcga
    11461 ataataagtt taaaataaat gcaccaataa atattgaatt taaaataata ttagaatgct
    11521 tgagtgcaat ggataataac tctatcattt gaaacctccc atcgaaaatc ttaaacgagt
    11581 agtatatctt attaattgta tcatgaagca atttaaatat catgacaatt tagtttgaaa
    11641 aacacacttt gtgttgacgt tgtgttttat gggatatagt atagtaagga atgtaaatga
    11701 aggagtgaat gctatgactg aacaagataa tgcacatcat tctgaacaaa taaaaacgaa
    11761 tcttaaatca cgtttaaatc gaattgaagg acaagtgaga gcgattaatc gcatgattga
    11821 agaagatgtc tattgtgatg atgtccttac gcaaataaga gcgacacgtt cggcgttaaa
    11881 cagtgttgcg ataaagttat tagaacaaca tatgaaaagt tgtattatga ataaagttaa
    11941 tcaaggtgct caggaagagg caatggaaga gttattagtg acttttcaaa aattgattaa
    12001 agactaaagg agtttaagat gatacatcaa aatacgattt acacagcggg aattgaaaca
    12061 gaagaacaag taagtcaatt gacagaacgc atttcaaata tgataggtgt tcatcaagtg
    12121 aatattaata taatagatgg tcaagtaact gtatcgtatg agacaccagc aaatttgaat
    12181 agtattgaaa aagaaatcta tgatgaagga tacaaaattg tattttaggg tataatgtag
    12241 agtgcgctat agattttaat tttgaaaata aattaaaaat tttgtaaatg atgtagtaaa
    12301 ggtatgtcga atagggtaag ataatattga aagcgatcca aacgaggtga taataatgaa
    12361 acgtcctgaa aagattcaaa atgtagtcaa actattgtca tcattaggtg tgaatattaa
    12421 aaaaactaaa tctcgtttag acattattaa tactttgcca gcatctaata aagtaagtca
    12481 cgaattaaaa taattgactg tgattgtgaa tcacacaccg ttaaagctat cgtcggacta
    12541 ttcgatgata gcttttttga ctggatttac taaaatgaaa gcaagtacta tataacaaaa
    12601 tccctcttta gagtgtacat tttacaatgt gtacttcaaa gagggattgt tattatcgag
    12661 taatatctat atatgacatt ttaaatcgac gacgtaaata gactatataa cgtcatcttg
    12721 aaaagttatg atatttgaaa ctcaaaatag tttaaattaa ttactttttg gatggtataa
    12781 atcgacatat cgttttggtt catgataata aattgataag actataccta ttccagtcat
    12841 catactccat agcgcactac caccataact aataaatggt aatggaatac cagtgattgg
    12901 taacaactga attgtcatac caatattttg taaaatatgg aacacaagta aagtgacgaa
    12961 accaacgata aagattttgt taaattgatc ttcaattttc gcagctaatc ttattagatg
    13021 gaagattaaa aataaaaata ttaagatcaa tatgacagaa ccgataaagc caagttcctc
    13081 tccaatcact gaaaagataa agtcagtatg attttcaggt atataaactt caccgtgatt
    13141 gtatccttta cctagtaact gtccagaacc gatagcttta agtgattcag ttaaatgata
    13201 gccatcacca ctactatatg tataggggtc aagccatgaa ttgattcgtc ccatttgata
    13261 cagttggaca cctaataaat tttcaattaa tgcgggtgca tatagaatac ctaaaatgac
    13321 tgtcattgca ccaacaatac ctgtaataaa gataggtgct aagatacgcc atgttatacc
    13381 acttactaac atcacacctg caataatagc agctaatact aatgtagttc ctaggtcatt
    13441 ttgcagtaat attaaaatac ttggtactaa cgagacacca ataattttga aaaataataa
    13501 caaatcactt tggaatgatt tattgaatgt gaattgatta tgtctagaaa cgacacgcgc
    13561 taatgctaaa attaaaataa ttttcatgaa ttcagatggc tgaatactga tagggccaaa
    13621 cgtgtaccaa cttttggcac cattgataat aggtgtaata ggtgactcag gaataacgag
    13681 caagcctatt aataatagac agattaagaa atacaataaa tatgtataat gtttaatctt
    13741 tttaggtgaa ataaacatga tgatacctgc aaaaattgca cctaaaatgt aataaaaaat
    13801 ttgtctgata ccgaaattag cactgtattg accaccgccc attgccgagt taataagcag
    13861 aacactgaaa attgctaaaa cagctatagt ggctactaat acccagtcta ctttgcgaag
    13921 ccaatgctta tccggctgtt gacgagatga ataattcatt gcaaactcct tttatactca
    13981 ctaatgttta tatcaatttt acatgacttt ttaaaaatta gctagaatat cacagtgata
    14041 tcagctatag atttcaattt gaattaggaa taaaatagaa gggaatattg ttctgattat
    14101 aaatgaatca acatagatac agacacataa gtcctcgttt ttaaaatgca aaatagcatt
    14161 aaaatgtgat actattaaga ttcaaagatg cgaataaatc aattaacaat aggactaaat
    14221 caatattaat ttatattaag gtagcaaacc ctgatatatc attggaggaa aacgaaatga
    14281 caaaagaaaa tatttgtatc gtttttggag ggaaaagtgc agaacacgaa gtatcgattc
    14341 tgacagcaca aaatgtatta aatgcaatag ataaagacaa atatcatgtt gatatcattt
    14401 atattaccaa tgatggtgat tggagaaagc aaaataatat tacagctgaa attaaatcta
    14461 ctgatgagct tcatttagaa aatggagagg cgcttgagat ttcacagcta ttgaaagaaa
    14521 gtagttcagg acaaccatac gatgcagtat tcccattatt acatggtcct aatggtgaag
    14581 atggcacgat tcaagggctt tttgaagttt tggatgtacc atatgtagga aatggtgtat
    14641 tgtcagctgc aagttctatg gacaaacttg taatgaaaca attatttgaa catcgagggt
    14701 taccacagtt accttatatt agtttcttac gttctgaata tgaaaaatat gaacataaca
    14761 ttttaaaatt agtaaatgat aaattaaatt acccagtctt tgttaaacct gctaacttag
    14821 ggtcaagtgt aggtatcagt aaatgtaata atgaagcgga acttaaagaa ggtattaaag
    14881 aagcattcca atttgaccgt aagcttgtta tagaacaagg cgttaacgca cgtgaaattg
    14941 aagtagcagt tttaggaaat gactatcctg aagcgacatg gccaggtgaa gtcgtaaaag
    15001 atgtcgcgtt ttacgattac aaatcaaaat ataaagatgg taaggttcaa ttacaaattc
    15061 cagctgactt agacgaagat gttcaattaa cgcttagaaa tatggcatta gaggcattca
    15121 aagcgacaga ttgttctggt ttagtccgtg ctgatttctt tgtaacagaa gacaaccaaa
    15181 tatatattaa tgaaacaaat gcaatgcctg gatttacggc tttcagtatg tatccaaagt
    15241 tatgggaaaa tatgggctta tcttatccag aattgattac aaaacttatc gagcttgcta
    15301 aagaacgtca ccaggataaa cagaaaaata aatacaaaat tgactaactg aggttgttat
    15361 tatgattaat gttacattaa agcaaattca atcatggatt ccttgtgaaa ttgaagatca
    15421 atttttaaat caagagataa atggagtcac aattgattca cgagcaattt ctaaaaatat
    15481 gttatttata ccatttaaag gtgaaaatgt tgacggtcat cgctttgtct ctaaagcatt
    15541 acaagatggt gctggggctg ctttttatca aagagggaca cctatagatg aaaatgtaag
    15601 cgggcctatt atatgggttg aagacacatt aacggcatta caacaattgg cacaagctta
    15661 cttgagacat gtaaacccta aagtaattgc cgtcacaggg tctaatggta aaacaacgac
    15721 taaagatatg attgaaagtg tattgcatac cgaatttaaa gttaagaaaa cgcaaggtaa
    15781 ttacaataat gaaattggtt tacctttaac tattttggaa ttagataatg atactgaaat
    15841 atcaatattg gagatgggga tgtcaggttt ccatgaaatt gaatttctgt caaacctcgc
    15901 tcaaccagat attgcagtta taactaatat tggtgagtca catatgcaag atttaggttc
    15961 gcgcgagggg attgctaaag ctaaatctga aattacaata ggtctaaaag ataatggtac
    16021 gtttatatat gatggcgatg aaccattatt gaaaccacat gttaaagaag ttgaaaatgc
    16081 aaaatgtatt agtattggtg ttgctactga taatgcatta gtttgttctg ttgatgatag
    16141 agatactaca ggtatttcat ttacgattaa taataaagaa cattacgatc tgccaatatt
    16201 aggaaagcat aatatgaaaa atgcgacgat tgccattgcg gttggtcatg aattaggttt
    16261 gacatataac acaatctatc aaaatttaaa aaatgtcagc ttaactggta tgcgtatgga
    16321 acaacataca ttagaaaatg atattactgt gataaatgat gcctataatg caagtcctac
    16381 aagtatgaga gcagctattg atacactgag tactttgaca gggcgtcgca ttctaatttt
    16441 aggagatgtt ttagaattag gtgaaaatag caaagaaatg catatcggtg taggtaatta
    16501 tttagaagaa aagcatatag atgtgttgta tacgtttggt aatgaagcga agtatattta
    16561 tgattcgggc cagcaacatg tcgaaaaagc acaacacttc aattctaaag acgatatgat
    16621 agaagtttta ataaacgatt taaaagcgca tgaccgtgta ttagttaaag gatcacgtgg
    16681 tatgaaatta gaagaagtgg taaatgcttt aatttcatag agattagtcg agggaccttt
    16741 tacttataaa aatgatttga attaatacta aaagattaca aagaagaggt ggttttgtgt
    16801 gtaaatacaa aattgccttt ttctttttat gttaaatcta taaatttgaa actaaatcaa
    16861 ggttaattct atgtacacac tttatatagg aagtagtttg aatgtttata taatgtttta
    16921 caaaaagatg tagtattata atgtctaatt tcacatgtgt ttcagtaaaa tttgttgtgg
    16981 aatgttaacg atatacgtat tttataaaaa attttttata atgattattc gaatgatgcg
    17041 taacgcttac atcttatcta atgctagctt tttgacaaaa atatgacaat caattaatgt
    17101 gattctaata aatattcgca aattgcttta ttgcgattaa atttttttgg tggtactata
    17161 tagaagttga tgaaatatta atgaacttat atgcaaaagt atattgagaa ataaacaggt
    17221 aaaaaggaga attattttgc aaaattttaa agaactaggg atttcggata atacggttca
    17281 gtcacttgaa tcaatgggat ttaaagagcc gacacctatc caaaaagaca gtatccctta
    17341 tgcgttacaa ggaattgata tccttgggca agctcaaacc ggtacaggta aaacaggagc
    17401 attcggtatt cctttaattg agaaagtagt agggaaacaa ggggttcaat cgttgatttt
    17461 agcacctaca agagaattgg caatgcaggt agctgaacaa ttaagagaat ttagccgtgg
    17521 acaaggtgtc caagttgtta ctgtattcgg tggtatgcct atcgaacgcc aaattaaagc
    17581 cttgaaaaaa ggcccacaaa tcgtagtcgg aacacctggg cgtgttatcg accatttaaa
    17641 tcgtcgcaca ttaaaaacgg acggaattca tactttgatt ttagatgaag ctgatgaaat
    17701 gatgaatatg ggattcatcg atgatatgag atttattatg gataaaattc cagcagtaca
    17761 acgtcaaaca atgttgttct cagctacaat gcctaaagca atccaagctt tagtacaaca
    17821 atttatgaaa tcaccaaaaa tcattaagac aatgaataat gaaatgtctg atccacaaat
    17881 cgaagaattc tatacaattg ttaaagaatt agagaaattt gatacattta caaatttcct
    17941 agatgttcat caacctgaat tagcaatcgt attcggacgt acaaaacgtc gtgttgatga
    18001 attaacaagt gctttgattt ctaaaggata taaagctgaa ggtttacatg gtgatattac
    18061 acaagcgaaa cgtttagaag tattaaagaa atttaaaaat gaccaaatta atattttagt
    18121 cgctactgat gtagcagcaa gaggactaga tatttctggt gtgagtcatg tttataactt
    18181 tgatatacct caagatactg aaagctatac acaccgtatt ggtcgtacgg gtcgtgctgg
    18241 taaagaaggt atcgctgtaa cgtttgttaa tccaatcgaa atggattata tcagacaaat
    18301 tgaagatgca aacggtagaa aaatgagtgc acttcgtcca ccacatcgta aagaagtact
    18361 tcaagcacgt gaagatgaca tcaaagaaaa agttgaaaac tggatgtcta aagagtcaga
    18421 atcacgcttg aaacgcattt ctacagagtt gttaaatgaa tataacgatg ttgatttagt
    18481 tgctgcactt ttacaagagt tagtagaagc aaacgatgaa gttgaagttc aattaacttt
    18541 tgaaaaacca ttatctcgca aaggccgtaa cggtaaacca agtggttctc gtaacagaaa
    18601 tagtaagcgt ggtaatccta aatttgacag taagagtaaa cgttcaaaag gatactcaag
    18661 taagaagaaa agtacaaaaa aattcgaccg taaagagaag agcagcggtg gaagcagacc
    18721 tatgaaaggt cgcacatttg ctgaccatca aaaataattt atagattaag agcttaaaga
    18781 tgtaatgtct tgagctcttt tttgttttca ataattgatt ctctgtagat atcaaagtac
    18841 taacgtttta aaggttaaat atttaattgg attgagatct gtatgcggtt atatcattct
    18901 gtgtaaatat ggttctccac caaatgtggt gagtatataa tttaaagaac tatttttaaa
    18961 ttaagaataa tcgaacataa ataaacttta tgaaatttca gtatcatgtt cttataaaaa
    19021 acaatagggc tttttgctga cgctagtgcg cgataaataa taagttgaat ataaaaaaga
    19081 tcactgccaa tcattcgttt aatggcagcg atctttttta tttaattatt tctctttcca
    19141 ctgcaacatt tgataaccaa tgcgtggatg tgttttaata atatcttttg cgtcctcatg
    19201 acattgtgaa agtttttgtc ttaatgatgt catatgcact cttaaactag gcatttcagt
    19261 cttattaaca tagccatata ttcgttttaa tatcatctca taagtgagta cttttccttt
    19321 atgatttgac aatagttcta acaagctaaa ttcatttggc gtcaaatgta cctcctgatt
    19381 attaataaca acagatttgg agccaaagtc gatgcttagc aaaccgttag taaatacaat
    19441 gttagtttct tgatgtgact tagcgattct ctcgatgact cgtattcgtg cccgaagctc
    19501 atcaacatta aaaggtttag tcatatagtc attcgcaccg ttatctaaag cttgaataat
    19561 tgtttgttct tcttgtcttg cacttattac aatgatagga atgtcagtat gttgcctgat
    19621 ttctgaaatc aaacataatc catctttatc tggtaaacct aaatctaata aaatgacatc
    19681 tggtttatca atttgaattt taaagtgtgc ttgtgtggca ttgtcggctg tagttacatt
    19741 gtaataatct aaagttaatg caacatcaag taaatgtgtg attgcgtgat catcttcaat
    19801 tatcaatatt ttagattgca ttatacgtct ccttcgttaa agtctgtata tatattgaaa
    19861 tagaatatac tgccgtgtgg ttggttcggt ttatattgta agtttgattg atgtttgtgt
    19921 aggatagtct gtactaaata taagcctagt cccatgcttt ctttttggtt atctttaaaa
    19981 tatttatttg atcctgtgta aaaaggctcg aatatctttt gttgttcttc taaactaatt
    20041 ccaggtcctt cgtctataac ggcaaattcg atttgttcat agctagcata acgaatagat
    20101 aaattgattt tggtgtcagt agaagtgtgt ttaactgcat tttcaatcaa attgaataaa
    20161 gcttgtaaaa tcaacttact gtcaatgtgt ataaactgta aatttactga ggatgataca
    20221 gttatacgct tttttaaatg gcgacgttct aaaatcatat cgatttcttc tactaattca
    20281 ctaacgagat aaggttgcaa ttttatctga acatttgatg actgtaattt tgttaatgat
    20341 aaaatatttg tcactaatag atataaatac tgactttctt gaaaactatg tacaagtaat
    20401 tgttcctttt ctatgataga catatcttta ctatgtgata ctaaaatatc taaatttccc
    20461 ataattgttg ttaacggtgt acgtatgtca tgcgaaattg atcttaaaaa atttgaatgt
    20521 gtcagttgac gttcagcctg taacatggat tctctcgttt gtttaagtaa cgtcacattt
    20581 tcaacggcga gagaaagttc atttaacatt gattctaata ttgatgcatc atatggatta
    20641 atcacttgag aactttggta atcaatggct agaatgcctt taatcggaga tgtgccaatt
    20701 ggtatcaacc atttattaat gcctggaaat gtatctgttg ttgcaccagc ttgtctttca
    20761 tttttaatta cccagcttaa tgcttgttca tgctgttgag tcgtattatc gatatggttt
    20821 tgcaatggta ttgttttaat tactttcgat tgattgataa cgtatatagt aattgattgt
    20881 tgcaataatt gattaatttg gtatccagca tttattagta agttttcaac tgtataagtt
    20941 tgtttaatcg aatcattaaa ttgaaataat aaatctgtac gataaagttg ctttttagta
    21001 atggagtatt ggaatttaat ttgttttaat aaagcactcg ttaaaatact tgttaaaatg
    21061 ctaacgataa atgtaatagg atagtcaaag cgatatactt caaatgtata tctaggttcc
    21121 gtaaaaaaat aattaaaaac aaatacgtta ataattgctg ctaaaaagcc aatgatgaag
    21181 gaccgcgtcc aaatggataa taaaatgatg ccgatgagaa aaatcattaa aataatcgta
    21241 ctagactcat gcttatcaag ttgataaatc cacagtccca tcattacaca aattatttga
    21301 atcaaaatca tttttaacat atctatggcg aaacgtttgc ctttaggacg atagggtgat
    21361 ttgttcattt tcaattcaac aggtatttgt ttgattggaa cgatttcgat tttatagcta
    21421 tgttcaaagg acattaaatg gtcaattaaa ggtgtattga aaaagtctcg ccacttattt
    21481 ctaatatgtt gtccgataat taatttggtt acatcttgat ttttacacca ttcgtctaat
    21541 cctaatgcaa cggtttggct ataaactact tttacttttg ctcctaatga ttttgcaagc
    21601 atgagatgtt gatgcacttg cttttgacta tctttatatt gcctgttttt ttcgaataca
    21661 tctatataaa tagcagtgaa cttcgcatgt tctttttgag caatatggaa tgcctcttta
    21721 attactgctt cattataaat gctcccacta attgccacag caatatgagg tttgagtgac
    21781 gttttatggt tgtgtcggac tttttcttta tcactcatca agtcggcaac tgttcttaac
    21841 gtcaatgtac gcagttcgct taggtgggca tacgtaaaga aattactaaa tgctacatct
    21901 agcctatcct ttttatatac cttgccagct tttaagcgtt taattaattg ttcaggtgag
    21961 atatctacga cttctaatac atcggcgctc attatgaaat agtcgggtac acgttcttta
    22021 acatgtacac cggtcatcag ttcaatttga ctacttaaac tttcaatatg ttgaatgttc
    22081 aaagtggtat gaacatcgat accatgattt aaaatttctt caatatccat atatcgtttc
    22141 tcatgacgat ctctagaaat attcgtatgt gctaactcat cgataagtac tattgtcggc
    22201 gattcttcga ttatgcggtc gacatctaaa tattgaaaat gatgactacc atgtttagta
    22261 aaattggttg tgattttagg taattgttca gctaatgcat ttgtttcatc acgctgatgc
    22321 ggttcaatat atcctatttt aatatcaacg ttactttgaa atagttcaat ggcatttgaa
    22381 agcatctcaa atgttttacc aacacctgga ctgtaaccga tgtaaattgt taaagatcca
    22441 cgcttttttc ctatgtttag cgattcagtg tttgacataa ccttcacctc gatagcaata
    22501 tcataatatt cttcacaatt aaatgctata aaatttattt ctgcatacac atcttaatga
    22561 tttcttaata actaagtttg aaaattaata tttctctcat aaacaagttg ttacgatgtt
    22621 gttgtgttgt aaggaggtta tggcaatgct catcgtttta ttactggtag ttattgcatt
    22681 agttttgtat ttattttatg ccttaattcg tagtgaaaaa ttttagaaat atattaagtg
    22741 agggttatca tggaaataat tttgtttcta acaatgatgg ttatgattac gtatgttttt
    22801 agtggatatc tatatcgcgt agcattggtg caatcgtcaa gagtagattt aatatttact
    22861 agatttgaaa atatgtgttt taaaattatc ggcacagatt tagaacacat gtcagctaaa
    22921 acatatgtca aacattttct ggcatttaat ggatttatgg ggtttataac atttgtattg
    22981 ctaatagttc agcaatggct ttttttaaat ccaaatcata atttaaacca atcgatagat
    23041 ttagcgttta atacagcaat atctttttta acaaatagta atttacaaca ctataacggt
    23101 gaatcagatg tgacatattt aacgcaaatg attgtaatga catatttaat gtttacatct
    23161 agtgcatcag gttacgccgt ttgtatagcg atgttgagac gtttaactgg attaactaat
    23221 atcattggta atttttatca agatattgtt cggtttattg tccgagtact tttaccatta
    23281 tcatgtttaa ttagtatttt attgatgact caaggtgtac cacaaacgtt gcatgctaat
    23341 ttaatgattc ggactttaag cggacatatt caacatattg catttggacc tattgcatca
    23401 cttgaatcaa taaaacatct tggtacgaat ggtggaggat ttttagcagg aaattctgca
    23461 acaccttttg aaaatccaaa tatttggagc aattttatag aaatgggcag tatgatgtta
    23521 cttcctatgt caatgttgtt cttatttggt cgcatgttaa gtagacatgg taaacgagta
    23581 catcgtcatg cgttgatatt atttgtcgca atgtttttca tttttatagc aattcttaca
    23641 ttaactatgt ggagtgagta tcgtggtaat ccaatactag cgaatttagg catttatgga
    23701 ccgaatatgg aaggtaaaga ggtacggttt ggagcaggtt tgtcagcact atttacagtt
    23761 attacgacgg catttacaac gggttctgtt aataacatgc atgatagctt aacgcctata
    23821 ggtggattag gaccaatggt attaatgatg ctaaatgttg tatttggtgg cgaaggcgta
    23881 ggactcatga atttattgat atttgtctta ctgacggtgt ttatatgcag tttgatggtt
    23941 ggtaaaacac cagaatattt aaatatgcca attggcgccc gtgaaatgaa atgtattgtc
    24001 ttagtctttc tcatacaccc aattttaatt ttagtatttt cagcacttgc ttttatgatt
    24061 cctggagcaa gtgaaagtat aacgaatccg tcttttcatg gtatttcaca agttatgtat
    24121 gaaatgacat cagctgctgc gaacaatgga tcagggtttg aaggactgaa agatgataca
    24181 acattctgga atatctctac aggaatcatt atgttgcttt ctcgttatat accaattatt
    24241 ttgcaattaa tgattgcatc aagtttagtg aataaaaaat cataccatca agataaatat
    24301 acaatagcga ttgataaacc ttattttggc gtatcactaa tcgtatttat cgttttacta
    24361 agtggtttga catttattcc agtactatta cttggtccta ttggtgaatt tttaacttta
    24421 aaataaagta aagggatgga aaaaatgcat catgtaaata aatatttcaa tcaaacaatg
    24481 gtgatagaag ctttgaaaat gagtttttat aagctgaatc ttaaacaatt aataaaaaat
    24541 ccgataatgt ttgtcgttga ggttggaatg attttaacgt tgatattaat atgttttcct
    24601 gacatatttg gaactagcta tctatcgcga ggttatttga ttacaatttt tatcatatta
    24661 ttaataacta tattgtttgc taatttttca gaagcctttg ctgaaggtcg tggaaaagcg
    24721 caagctgata gtttaagaca agcacaatca aatttaactg ctcgattgat agaagaaaat
    24781 ggtgcgtatc ggatagtgaa cgccacagag ttaaaagccg gacaaaatat tcgggtagaa
    24841 aatggagaaa caatccctgc tgatggcgtt gtaataaatg gattagcaac cgttgatgaa
    24901 tctgcaatta ctggtgagtc tgcacctgtg ataaaagaat caggtggcga ttttgatgga
    24961 gtcattggcg gtacacttgt aacatctgat tggttagaaa tacgtgttga gagtgaagcg
    25021 ggtacatctt ttttagataa aatgattgcc ttagttgaag gggcggaaag aaataaaaca
    25081 ccaaatgaaa ttgcactttt tacattatta acaacgttaa caattatatt tttagtcgtt
    25141 attgtaacgt tatatccaat agcatcttac ctgcatttaa ttttacctat tgcgatgctc
    25201 attgcgttaa cagtatgttt aataccaacg acaatcggtg gtttgttatc ggcgattggt
    25261 attgcaggca tggatcgtgt tactcaattc aatgtattag cgaaaagtgg gcgtgctgta
    25321 gaagtatgtg gagatgtcga tgtaatgatt ttagacaaga caggcacaat cacttatggc
    25381 aatcgaatcg ctagtgaatt tttaccagtg aatcaacaaa tgcttgaaaa attgatagtc
    25441 gctgcttata tgtcatcaat ctacgatgat acgccagaag gaaaaagtat tgtacgatta
    25501 gcgaagcaga tgtatataaa tgagctgcct aaagacattg atggcacata taagcctttt
    25561 acagctgaaa ctagaatgag cggaatcatt acaaatgaaa tatcggtatt caaaggtgca
    25621 cctaatagta tgataaatct tgtcaaacaa caacaaggca atattcctct taatattgag
    25681 tctctctgta tggatgtatc gagtaaaggt ggcacaccat taatagtcat tgaaaacaat
    25741 gtcatgcttg gcgtgattta tttaaaagat gtgattaaag atggtcttgt agaacgattt
    25801 actgaattga gaaaaatggg gattgaaaca gttatgtgta caggtgataa tgctttaaca
    25861 gcagctacaa ttgcaaaaga agccggggtt gatagatttg ttgcagagtg taaacccgaa
    25921 gataaaatta aagtcatcaa agatgaacaa gcgaaaggcc atattgttgc gatgacagga
    25981 gatggtacaa atgatgcacc tgcgctagca caagcgaata taggattagc tatgaactca
    26041 gggacaatta gtgctaaaga ggcagctaat ctaattgatt tagattcgaa tcctacaaag
    26101 ttaatagagg ttgtaaaaat aggcaaacaa ttattaatga cgagaggtgc attaacgaca
    26161 tttagtttgg ctaatgacgt agcaaaatac tttgcaattt tacctgcact aatgatgtca
    26221 acaattccag aaatgacttc actaaatatt atgcatttat cgtcacctaa atctgcaatt
    26281 atatcagctt tgatattcaa tgcattaatc attgttgcct taattccaat tgccatgaaa
    26341 ggtgtaaaag taaagggtta cagcattgat agaatcttta taaataatat gttgatttat
    26401 ggattaggtg gattaatcgt cccgttttta ggcattaaat taatagatat gattgtacaa
    26461 tttttcgtgt aaagaggaag gagcctgtac catgaatacg ataagaaata gtatttgttt
    26521 aacgataata actatggtat tatgtggatt tttattcccg ttagctatca cgttaattgg
    26581 tcaaatattt ttttatcaac aagcaaatgg cagtttaatt acgtatgaca atcgtatagt
    26641 tggatcaaaa ttgattggac agcactggac agagacacga tattttcacg ggagaccgag
    26701 tgcagttgat tataatatga atcctgaaaa attatataaa aacggcgtat cttcaggtgg
    26761 aagtaatgaa tcgaatggaa acaccgaatt aatagcacgc atgaaacatc atgttaaatt
    26821 tgggaattca aatgtaacaa tagatgctgc aacaagttct gggtcgggct tagatccgca
    26881 tattacggtc gaaaatgcat tgaagcaagc acctcgtatt gctgatgctc gtcatgtatc
    26941 tacttcaaga gtggccgact tgattcaaca tcgtaagcag cgtggtgtgt taacaaatga
    27001 ttatgtgaat gtgcttgaac taaatatcgc tcttgataag atgaaagatt gattatagat
    27061 gaaaggcatc atgttgataa aattataaca tggtgtcttt ttagcaattc aagcactttc
    27121 tgcttaatta aatttttaga tgtaccttta ggaagaataa attagcatga aatattccat
    27181 atgctataat tatcatcata taaaaataaa atgaaataga ttataaatcc gtataatttt
    27241 ttatttgggg agaggggtat atgtcagatt atactaagtt gactaagagt cctaagtcag
    27301 cattgttgta ttactacatt acgaatggct tagaatttat attgagtgta gctgtgtatg
    27361 tcatttttta ttttatatgg ttaagatttg aatggccaca ttacttactt tatattttgt
    27421 tggttttagg tgctctgagt acattaaaat tatttattaa gcctttatgg caatatcatt
    27481 gtcgctttta taaagttgat caactaagtg tacaatatcg tacatcattt tttatatata
    27541 aagaagaaac gagtcgaatt gaacgtttac aatacttgtc gattaaatct aatccaatta
    27601 gtaaagtgct taatttgtat aaagttggat ttatgactgc aggtcatact atttatttac
    27661 cgatgatgtc tcatgacgat gttaaaataa tcgaagagag aactatgtcg aatttgagag
    27721 gggttgaaag tgatgtctga acctcaaaaa ttacatccga tttcatattt taatggcata
    27781 atcaatgcta tcaaacaaaa catagtcgtc ttttttattt tcattatctt tcaattgaaa
    27841 gattttgatt atacgaatcc cgaatcatat ttatggatag gtattgtatt tgtattcttt
    27901 ttaatatcat acatttctca aattgtcgga attatgaaca cgcgatattg gattgaggat
    27961 aattatttca ttttaacaac aggtattttt aataaaaagc gtaaagaatt aaatataaaa
    28021 cgtattcagt ccgttgatat gacacaaggg gtcgtcaatc aaattattgg tggtgtggat
    28081 ttacaaatca aaacaccaag tgatggtatt gtcttaagtg ttatttcgaa aaaacaaggt
    28141 gaatatcttg aacgatatat tgatcaattg caaacagagt tgaagacaga agtggcgcaa
    28201 aagtctagtg ctgagaagaa tgacaactgt aatcatgaca ttgaaggtaa tgtggaagac
    28261 atcgcagaag gtcgcttaga tgaagtgcag aacactgata gtatgcttaa tcagaaaaat
    28321 aaacgcattc ctatatatca gatgaatttt aaagaatcac tatttatggc aatgaccagt
    28381 ggcgccattg gtgtaacgtt agcagcttta gtaccgattt acggggctgt aagagagttg
    28441 atcccatggt cgaagctaaa tcatgaaatt agtcaatggg ttcaaattgt aagtatttca
    28501 gtattaatca tcattttact agtattaata gtggcatata ttattggaac gattataaca
    28561 atgataagat tttatggctt ttcggtgatg cttgaaaatg agcaacttaa aattgaatat
    28621 ggtctcttta acaagaagaa agtaactgta ccaacgaaac gattgcaagc tgttgttgaa
    28681 cgtcagtcat atatacgaag attatttggc tacacagcaa tacattttat catcacaagt
    28741 gattttgaaa ataaaaaaat cgaagatgat gacaatgatt caggtagtgt gccgatattg
    28801 ccgtttatta aacgagacaa agcatatcaa attatccaga atttagtacc agatttatct
    28861 tatcaacaag ttaatgaagg tatgcctttg tcaggtttcc acagacactt tttaatacct
    28921 agtattattg tcatagctat ggcaggtata ggctgttatt tttggaatca attattattg
    28981 ttatttacga ttataacaat agtcattatt ggtttatttg tgatcaaagg atatctttat
    29041 gtcaaacatg cgggttttaa aatgacagaa gaacaaatta ctatccagaa tttcgagcta
    29101 ttgaaacgaa atatttatta ttttaaagcg catcacatta taggatttga cacttggcaa
    29161 catacattac ttaaacgcaa caatttaaat aattttagct ttattgttgc taaaggtgtg
    29221 ggagcccaat cgattaactt aaaatacgca agaagtgaag atgcaaataa attgaaatca
    29281 tggtatttaa gaggtgaaaa tgatgaggga ttataatttt atgtcaccac acgctaaaaa
    29341 agtgatgagg ttaagtgctg tgctgttttg gatacccata gcgcttgtac ttttaatcgc
    29401 atttaatatt ttgaactggc tattttggga gattttagat aaccatttat ccattgttag
    29461 tagtgtgatt ataatattac ttgttgctgt tttcacgctt gttatagcgc cagtctatcg
    29521 atataagcac tgcagatatg catttgaaaa acaccatttg cgtgtacgaa acggcatttt
    29581 atttttggat gaaaaggtaa taccatattt tcgtattcaa aatattgata tcgatgttgg
    29641 tcctataatg agacgctatc gattagcaac tttgaccctt tatacagcgg gtggtcaagc
    29701 tgaaattgaa ttaatagata gaagtgaagc acgtagattg aaacgatggt taaataacga
    29761 gagacattta gaaaacaata aaaatgagga ataatcaatg atacatggaa ttggtgtaga
    29821 tttaatcgaa atcgatcgaa tacaagcgtt atatagtaag caaccaaaat tggttgagcg
    29881 gattttaact aaaaatgaac agcacaaatt caacaatttc acacatgagc aacgtaaaat
    29941 tgaattttta gctggcaggt ttgctacaaa agaagcgttc agtaaagcat taggcacagg
    30001 cttaggaaaa catgtagctt ttaacgatat agactgttac aacgacgaac ttggcaaacc
    30061 aaagattgat tacgaagggt ttatcgtaca tgttagtatc tcacacactg agcattatgc
    30121 gatgagccaa gttgttttag aaaagtcagc attttaaatt tgttatacaa tgataattaa
    30181 gatctagttt taaaacgtgt gggaattaca ggaggaagta atatgtcgga taaatattat
    30241 agatctgcgt atatgaatgt agatttaaac gctgttgcat caaatttcaa agtattcagt
    30301 acattgcatc caaataaaac agtgatggct gtcgttaaag ccaatgccta tggactaggt
    30361 agtgttaaag tagcacgtca tttaatggaa aatggcgcca cattttttgc tgtagcaacg
    30421 ttagatgaag cgatagaact tagaatgcat gggattactg ctaaaatttt agtcttaggt
    30481 gtgttaccag ctaaagatat tgataaagcg atacaacacc gagttgcctt aacggttccg
    30541 tctaaacagt ggttgaaaga agcaattaaa aacatttctg gtgagcaaga gaaaaagtta
    30601 tggttgcaca ttaaattaga tacaggaatg ggacgtttag gtattaaaga tactaaaacg
    30661 tatcaagaag tgattgaaat cattcaacaa tatgagcaac ttgtatttga aggcgtgttt
    30721 acacactttg cctgtgctga cgaaccagga gatatgacaa ctgaacaata tcaacgtttt
    30781 aaagatatgg tcaatgaagc aattaaacct gaatatatac attgtcagaa ctcagcaggc
    30841 tctctattaa tggattgcca attctgtaat gcaataagac caggaatttc cctttatgga
    30901 tattatccat cagagtatgt acagcaaaaa gttaaagtac accttaaacc aagtgtgcaa
    30961 ttaattgcta atgtagttca aacaaagacg ctacaagcgg gtgagtctgt aagttatggt
    31021 gcaacttata cagctactga ctcaactaca atagcattgt tacctattgg atatgcagat
    31081 ggctatttac gcataatgca aggtagcttt gtaaatgtaa atggtcatca atgcgaagtt
    31141 attggtcgcg tatgtatgga tcagacaatt gttaaagtgc cagatcaagt taaagctgga
    31201 gattcggtga ttttaataga taatcataga gaaagtccac agtcggtaga ggtggtagct
    31261 gaaaagcaac atactattaa ttatgaagtg ctttgtaact tgtcgagacg tttgccgcga
    31321 atctatcatg atggtgatca acgttttgta acaaatgaat tgttaaaata atatggtcag
    31381 tgcatataaa atttgttatc attagagtaa ttaaaggtca tttaataact tttggaatca
    31441 attggaggtt ctcatatgtt atcttttagt caaaatagaa gtcatagctt agaacaatct
    31501 ttaaaagaag gatattcaca aatggctgat ttaaatctct ccctagcgaa cgaagctttt
    31561 ccgatagagt gtgaagcatg cgattgcaac gaaacatatt tatcttctaa ttcaacgaat
    31621 gaatgattag acgaggagat gtttatttag cagatttatc accagtacag ggatctgaac
    31681 aagggggagt cagacctgta gtcataattc aaaatgatac tggtaataaa tatagtccta
    31741 cagttattgt tgcggcaata actggtagga ttaataaagc gaaaataccg acacatgtag
    31801 agattgaaaa gaaaaagtat aagttggata aagactcagt tatattatta gaacaaattc
    31861 gtacacttga taaaaaacga ttgaaagaaa aactgacgta cttatccgat gataaaatga
    31921 aagaagtaga taatgcacta atgattagtt tagggctga
//
LOCUS       DAXWFK010000010        31847 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.010, whole genome shotgun sequence.
ACCESSION   DAXWFK010000010 DAXWFK010000000
VERSION     DAXWFK010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 31847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31847
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.010"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(47..424)
                     /gene="ygs"
                     /locus_tag="ACGEXS_000375"
     CDS             complement(47..424)
                     /gene="ygs"
                     /locus_tag="ACGEXS_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing post-transcriptional
                     regulator Ygs"
                     /protein_id="HFF8011423.1"
                     /translation="MNNYKIGQHIKVRVTGIQPYGAFVETPNHTEGLIHISEIMDDYV
                     HNLKKFLSEGQIVKAKILSIDDEGKLNLSLKDNDYFKNYERKKEKQSVLDEIRETEKY
                     GFQTLKERLPIWIKQSKRAIRND"
     gene            complement(840..1433)
                     /locus_tag="ACGEXS_000376"
     CDS             complement(840..1433)
                     /locus_tag="ACGEXS_000376"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFF8011424.1"
                     /translation="MANYPQLNKEVQQGEIKVVMHTNKGDMTFKLFPNIAPKTVENFV
                     THAKNGYYDGITFHRVINDFMIQGGDPTATGMGGESIYGGAFEDEFSLNAFNLYGALS
                     MANSGPNTNGSQFFIVQMKEVPQNMLSQLADGGWPQPIVDAYGEKGGTPWLDQKHTVF
                     GQIIDGETTLEDIANTKVGPQDKPLHDVVIESIDVEE"
     gene            1497..1880
                     /locus_tag="ACGEXS_000377"
     CDS             1497..1880
                     /locus_tag="ACGEXS_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase-associated lipoprotein B"
                     /protein_id="HFF8011425.1"
                     /translation="MKLYRFSHKTGSYGVTIVEENGDDVLVKVEQVIKHPKQGDLHNP
                     TETEGVFFHDRKALSHFEKRYTKRSQLRDFNVDVLRYEDSLQQAITDLETKIKAQQTK
                     HAEMSLASLARLKEDYSLQYKENFK"
     gene            2011..4416
                     /gene="mnhA1"
                     /locus_tag="ACGEXS_000378"
     CDS             2011..4416
                     /gene="mnhA1"
                     /locus_tag="ACGEXS_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit A"
                     /protein_id="HFF8011426.1"
                     /translation="MSLLHIAVILPLIFALIIPILYRFFKRIHLGWFVLPVPIVIFIY
                     MLTLIKTTMSGNTVMKTLNWMPHFGMNFDLYLDGLGLLFSLLISGIGSLVVLYSIGYL
                     SKSEQLGNFYCYLLLFMGAMLGVVLSDNVIILYLFWELTSFSSFLLISFWRERQASIY
                     GAQKSLIITVFGGLSLLGGIILLAIPTQSFSIQYMIQHASEIQNSPFFIFAMILIMIG
                     AFTKSAQFPFYIWLPDAMEAPTPVSAYLHSATMVKAGLYLIARMTPIFAASQGWVWTV
                     TLVGLITLFWASLNATKQQDLKGILAFSTVSQLGMIMAMLGIGAISYHYQGDDSKIYA
                     AAFTAAIFHLINHATFKGALFMITGAVDHSTGTRDVKKLGGLLTIMPISFTITVITAL
                     SMAGVPPFNGFLSKESFLETTFTASQANLFSVDTLGYLFPIIGIVGSVFTFVYSIKFI
                     MHIFFGQYKPEQLPKKAHEVSILMLLSPAILATLVIVFGLFPGILTNSIIEPATSSIN
                     HTVIDDVEFHMFHGLTPAFLSTLVIYILGILLIVTFSYWVKLLQRQPGKLTFNYWYNR
                     SANVIPNYSEKMTNSYVTDYSRNNLVIIFGALILLTFVTIFSVPFNINFKDVSPIRIF
                     EVCIVILLLSAAFLILFAKSRLFSIIMLSAVGYAVSVLFIFFKAPDLALTQFVVESIS
                     TALFLLCFYHLPNLNRYNEKHSFQLTNALIAGGVGLSVIIIGLIAYGNRHFESISKFY
                     QEHVYDLAHGKNMVNVILVDFRGMDTLFESSVLGIAGLAVYTMIKLRKKRQTQGNEVK
                     NHE"
     gene            4409..4837
                     /gene="mnhB1"
                     /locus_tag="ACGEXS_000379"
     CDS             4409..4837
                     /gene="mnhB1"
                     /locus_tag="ACGEXS_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit B"
                     /protein_id="HFF8011427.1"
                     /translation="MNRQQNDLILQFAAVIIFFMVMVFGFSLFLAGHYTPGGGFVGGL
                     LFASSLVIITIAFDIETMRKIFPLDFKILIGIGLVFCIATPIASWFLGKNFFTHVTFD
                     IPLFILEPVHMTTAVFFDFGVLCAVVGTVMTIIISIGENE"
     gene            4837..5178
                     /gene="mnhC1"
                     /locus_tag="ACGEXS_000380"
     CDS             4837..5178
                     /gene="mnhC1"
                     /locus_tag="ACGEXS_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit C"
                     /protein_id="HFF8011428.1"
                     /translation="MEIIMIFVSGILTAISVYLVLSKSLIRIVMGTTLLTHAANLFLI
                     TMGGLKHGTVPIYEANVKSYVDPIPQALILTAIVIAFATTAFFLVLAFRTYKELGTDN
                     VESMKGVPEDD"
     gene            5171..6667
                     /gene="mnhD1"
                     /locus_tag="ACGEXS_000381"
     CDS             5171..6667
                     /gene="mnhD1"
                     /locus_tag="ACGEXS_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit D"
                     /protein_id="HFF8011429.1"
                     /translation="MIESNMLVLTLVIPVITAILLVFIGKRPIIKRYVALGGTLLTLV
                     AAIINLANVVKHGPIRVELGSWKAPYSIVFVLDIFSALLIITSIIITAIVILYSYQTI
                     GIERERYYYYFSVLFMLIGIIGAFTTGDIFNLFVFFEVFLMSSYFLLVIGSTKIQLQE
                     TIKYVLVNVVSSSFFVMGVAILYSVVGTLNLADISNKLANLSAHDSGLVNIVFILFIF
                     VFATKAGVFPMFVWLPSAYYAPPIPIIAFFGALLTKVGVYAIARTLSLFFSDNVSFSH
                     YVILFLALLTIIFGCVGAVAYANIKKIILYNVMIAVGVILVGIAMMTESGMIGAIYYT
                     LHDMLVKLALFLLIGIMIKITGTADLRQFGGLIKRYPVLGWSFFIAALSLAGIPPLSG
                     FYGKFFIVQSTFERGFYLSGVIVLLSSLVVLYSVIRIFLQGFFGQPKGYDLNNKVDVK
                     YLTTIAIVAVVITVLYGLSADYLYPMVKAGAETFYNPSTYVKAVLGGK"
     gene            6669..7148
                     /gene="mnhE1"
                     /locus_tag="ACGEXS_000382"
     CDS             6669..7148
                     /gene="mnhE1"
                     /locus_tag="ACGEXS_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit E"
                     /protein_id="HFF8011430.1"
                     /translation="MAVQLVLNFIIAVFWLFVTNSYTTNNFVLGFIFGLVLVYLLHRV
                     LPGRFYVITLYRIIKLVIIFLIELIKANFDVLKIIIKPSIKNEPGFFVYHTDLKKDWQ
                     IVLLSNLITLTPGTVVLGVSDDRTKIYIHAIDFSTKEQEVESIKTSLEKIVREVGEI"
     gene            7148..7441
                     /gene="mnhF1"
                     /locus_tag="ACGEXS_000383"
     CDS             7148..7441
                     /gene="mnhF1"
                     /locus_tag="ACGEXS_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit F"
                     /protein_id="HFF8011431.1"
                     /translation="MNHNVIIVIALIIVVISMLAMLIRVVLGPSLADRVVALDAIGLQ
                     LMAVIALFSILLNIKYMIVVIMMIGILAFLGTAVFSKFMDKGKVIEHDQNHTD"
     gene            7419..7775
                     /gene="mnhG1"
                     /locus_tag="ACGEXS_000384"
     CDS             7419..7775
                     /gene="mnhG1"
                     /locus_tag="ACGEXS_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit G"
                     /protein_id="HFF8011432.1"
                     /translation="MIKIILISLALIFVIIGALISALAAIGLLRLEDVYSRAHAAGKA
                     STLGAMSLLFGTFLYFIATQGFVNMQLIVAIIFVLITGPLSSHMIMKAAYNIKTPYTK
                     KTKVDEISEDLKDTKL"
     gene            8024..9178
                     /locus_tag="ACGEXS_000385"
     CDS             8024..9178
                     /locus_tag="ACGEXS_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFF8011433.1"
                     /translation="MHWTIIGGGIQGTAIAQKLLSSGLTTDRLTIIDPHETFCQRFNS
                     YTNRIEMPYLRSPIVHHVHPQPFHLKQFAKQHQYTNAFYGPYQRPELTMFMDHIAHAS
                     KQYQLEDCLVQGLVQTLDKQEDKWHIKLEDGQIITTDCVVIAIGSTNIPFMPDILKDK
                     QNVNHIFEKELDQVVYDKTDHIVGSGITAAHLALKLLNHDNDKKIHLWLNKDIEIHDF
                     DADPGWLGPKNMSSFLSTKSMPERNAIVQRERHKGSMPHELYLRLKKHIKNGRINVHK
                     TPITQISGGVINTENDSVPYQQIMVATGFEQDFMSQPLIKQLIQNYDAPINECNYPVI
                     SEKLEWIPNLFVAGCFADLELGPFGRNVMGGRKAAERIEQAFLKLQQYSA"
     gene            complement(9234..9608)
                     /locus_tag="ACGEXS_000386"
     CDS             complement(9234..9608)
                     /locus_tag="ACGEXS_000386"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HFF8011434.1"
                     /translation="MTHLLETFEMSIDHQEDGLVVISMPVTDKVKQPFGYLHGGASIA
                     LGETACSLGSANLIDTTKFIPLGLEMNANHIHSAKDGRVTATAEIIHRGKSTHVWDIK
                     IKNDKEQLITVMRGTVAIKPLK"
     gene            complement(9627..10943)
                     /locus_tag="ACGEXS_000387"
     CDS             complement(9627..10943)
                     /locus_tag="ACGEXS_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HFF8011435.1"
                     /translation="MINAVVIAVILMIVLCLCRLNVVISLFISALVGGLISGMSIEKV
                     INVFGKNIVDGAEVALSYALLGGFAALISYSGITDYLVGKIINAIHAENSRWSRVKVK
                     VTIIIALLAMSIMSQNLIPVHIAFIPIVIPPLLSLFNDLKIDRRLIGLIIGFGLCFPY
                     VLLPYGFGQIFQQIIQSGFAKANHPIEFNMIWKAMLIPSMGYIVGLLIGLYVYRKPRE
                     YETRKISDSDNVTELKPYILIVTIVAILATFLVQTFTDSMIFGALAGVLVFFISRAYN
                     WYELDAKFVEGIKIMAYIGVVILTANGFAGVMNATGDIDELVKTLTSITGDNKLFSII
                     MMYVIGLIVTLGIGSSFATIPIIASLFIPFGASIGLDTMALIALIGTASALGDSGSPA
                     SDSTLGPTAGLNVDGQHDHIRDTCVPNFLFYNIPLMIFGTIAAMVL"
     gene            complement(11354..12829)
                     /locus_tag="ACGEXS_000388"
     CDS             complement(11354..12829)
                     /locus_tag="ACGEXS_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HFF8011436.1"
                     /translation="MNFKLNNTLSNEINTLIIGIPEHLNQLERISFNHIDITESLERL
                     KHQHIIGSKVGKIYTTAFDVQDQTYRLITVGLGNLKTRSYQDMLKIWGHLFQYIKSEH
                     IEDTYLLMDSFISKYDQLSDVLMACGIQSERATYEFDHYKSSKKAPFKTNLNLISESL
                     IELDFIHEGISIGQSINLARDFSNMPPNVLTPQTFAEDIVNHFKNTKVKVDVKDYDTL
                     VSEGFGLLQAVGKGSKHKPRLVTITYNGKDKDEAPIALVGKGITYDSGGYSIKTKNGM
                     ATMKFDMCGAANVVGIIEAASRLQLPVNIVGVLACAENMINEASMKPDDVFTALSGET
                     VEVMNTDAEGRLVLADAVFYANQYQPSVIMDFATLTGAAIVALGDDKAAAFESNSKVI
                     LNDILQISSEVDEMVFELPITATERASIKHSDIADLVNHTNGQGKALFAASFVTHFSG
                     QTPHIHFDIAGPATTNKASYNGPKGPTGFMIPTIVQWLKQQ"
     gene            complement(12960..14168)
                     /locus_tag="ACGEXS_000389"
     CDS             complement(12960..14168)
                     /locus_tag="ACGEXS_000389"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFF8011437.1"
                     /translation="MAQDRKKVLVLGAGYAGLQTVTKLQKAISTEEAEITLINKNEYH
                     YEATWLHEASAGTLNYEDVLYPVESVLKKDKVNFVQAEVTKIDRDAKKVETNQGIYDF
                     DILVVALGFVSETFGIEGMKDHAFQIENVITARELSRHIEDKFANYAASKEKDDNDLS
                     ILVGGAGFTGVEFLGELTDRIPELCSKYGVDQNKVKITCVEAAPKMLPMFSEELVNHA
                     VSYLEDRGVEFKIATPIVACNEKGFVVEVDGEKQQLNAGTSVWAAGVRGSKLMEESFE
                     GVKRGRIVTKQDLTINGYDNIFVIGDCSAFIPAGEERPLPTTAQIAMQQGESVAKNIK
                     RILNGESTEEFEYVDRGTVCSLGSHDGVGMVFGKPIAGKKAAFMKKVIDTRAVFKIGG
                     IGLAFKKGKF"
     gene            complement(14622..14981)
                     /locus_tag="ACGEXS_000390"
     CDS             complement(14622..14981)
                     /locus_tag="ACGEXS_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/IscA family protein"
                     /protein_id="HFF8011438.1"
                     /translation="MPTVILTEAAAYEVKDMLKANEMPDGYLKIKVNGGGCTGLTYGM
                     GAEEAPGENDEVLEYFGLKVLVDKKDAPVLNGTTIDFKQSLMGGGFQIDNPNAIASCG
                     CGSSFRTAKVAGNPENC"
     gene            complement(14994..15230)
                     /locus_tag="ACGEXS_000391"
     CDS             complement(14994..15230)
                     /locus_tag="ACGEXS_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuzB family protein"
                     /protein_id="HFF8011439.1"
                     /translation="MNPIVEFCLSNMAKGGDYVFNQLENDPDVDVLEYGCLTHCGICS
                     AGLYALVNGDIVEGDSPEELLQNIYAHIKETWIF"
     gene            15547..16611
                     /locus_tag="ACGEXS_000392"
     CDS             15547..16611
                     /locus_tag="ACGEXS_000392"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFF8011440.1"
                     /translation="MKNLVLLGGGYGNMRIMSRILTTSLPQDYTVTLVDRMPFHGLKP
                     EFYALAAGTKSDKDVRMKFPNHPQVNTVYGEINDIDLDAQIVSVGNSKIDYDELIIGL
                     GCEDKYHNVPGAEEYTHSIQTLSKARDTFHSISELPEGAKVGIVGAGLSGIELASELR
                     ESRSDLEIYLYDRGPRILRNFPEKLSKYVAKWFAKNNVTVVPNSNINKVEPGKIYNCD
                     EPKDIDLVVWTAGIQPVEVVVNLPIDINSNGRVIVNQYHQVPTYRNVYVVGDCADLPH
                     APSAQLAEVQGDQIADVLKKQWLNEPLPDKMPELKVQGIVGSLGDKQGFAYIMDRTVT
                     GRLASILKSGVLWLYKYHNG"
     gene            complement(16670..16993)
                     /locus_tag="ACGEXS_000393"
     CDS             complement(16670..16993)
                     /locus_tag="ACGEXS_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuzD family protein"
                     /protein_id="HFF8011441.1"
                     /translation="MEHVSVVVYGADVICASCVNAPTSKDIYDWLQPLLKRKYPNISF
                     KYTYIDITKDNDNLTDHDLQFIERIEQDELFYPLITMNDEYVADGYIQTKQITRFIDQ
                     KLVNE"
     gene            17094..17336
                     /locus_tag="ACGEXS_000394"
     CDS             17094..17336
                     /locus_tag="ACGEXS_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HFF8011442.1"
                     /translation="MPTEDTTMFDQVAEVIERLRPFLLRDGGDCSLIDVEDGIVKLQL
                     HGACGTCPSSTITLKAGIERALHEEVPGVIEVEQVF"
     gene            complement(17599..18774)
                     /gene="dltD"
                     /locus_tag="ACGEXS_000395"
     CDS             complement(17599..18774)
                     /gene="dltD"
                     /locus_tag="ACGEXS_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFF8011443.1"
                     /translation="MKLKPFLPILISGAVFIVFLLLPASWFTGLVNEKTVEDNRTSLT
                     DQVLKGTLIQDKLYESNKYYPIYGSSELGKDDPFNPAIALNKHNANKKAFLLGAGGST
                     DLINAVELASQYDKLKGKKLTFIISPQWFTNHGLTNQNFDARMSQTQINQMFQQKNMS
                     TELKRRYAQRLLQFPHVHNKEYLKSYAKNPKETKDSYISGFKENQLIKIEAIKSLFAM
                     DKSPLEHVKPATKPDASWDEMKQKAVEIGKADTTSNKFGIRDQYWKLIQESKRKVRRD
                     YEFNVNSPEFQDLELLVKTMRAAGADVQYVSIPSNGVWYDHIGIDKERRQAVYKKIHS
                     TVVDNGGKIYDMTDKDYEKYVISDAVHIGWKGWVYMDEQIAKHMKGEPQPEVDKPKN"
     gene            complement(18771..19007)
                     /gene="dltC"
                     /locus_tag="ACGEXS_000396"
     CDS             complement(18771..19007)
                     /gene="dltC"
                     /locus_tag="ACGEXS_000396"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002433359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     2"
                     /protein_id="HFF8011444.1"
                     /translation="MEFREQVLNLLAEVAENDIVKENPDVEIFEEGIIDSFQTVGLLL
                     EIQNKLDIEVSIMDFDRDEWATPNKIVEALEELR"
     gene            complement(19025..20239)
                     /gene="dltB"
                     /locus_tag="ACGEXS_000397"
     CDS             complement(19025..20239)
                     /gene="dltB"
                     /locus_tag="ACGEXS_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PG:teichoic acid D-alanyltransferase DltB"
                     /protein_id="HFF8011445.1"
                     /translation="MIPYGDFTFFLIALIALLPVIILGFLGKRSYIYNGVVTAFMIVL
                     IFSSDKHNLFDQKYLSVQLISFIIYVVWQVLLIMFYYHSKPKNNSFSKFVTVMVLSIL
                     PLALVKVLQSTWLGGHQIHFHESKLIEFVGFLGISYVTFKSVQLIMEIRDGSIKEIKV
                     WKLIQFISFFPTISSGPIDRYKRFVKDDKKVPTGNEYRELVLKAIHMIMLGFLYKYIV
                     AYFINTYAIMPLQLDLHGFVNLWLYMYAYSLYLFFDFAGYSLFAIAFSYLFGIKTPPN
                     FDKPFKAKNIKDFWNRWHMTLSFWFRDCIYMRSLFYMSRKKLLKSQFAMSNVAFLINF
                     FIMGIWHGIEVYYIVYGLYHAALFIGYGYYERWRKKHPPRWQNGFTTALSIVITFHFV
                     TFGFLIFSGKLI"
     gene            complement(20236..21693)
                     /gene="dltA"
                     /locus_tag="ACGEXS_000398"
     CDS             complement(20236..21693)
                     /gene="dltA"
                     /locus_tag="ACGEXS_000398"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFF8011446.1"
                     /translation="MTDIINKLQAFADANPQSIAVRHTTDELTYQQLMDESSKLAHRL
                     QGSKKPMILFGHMSPYMIVGMIGAIKAGCGYVPVDTSIPEDRIKMIINKVQPEFVFNT
                     TDESFESLEGEVFTIEDIKTSQDPVIFDSQIKDNDTVYTIFTSGSTGEPKGVQIEYAS
                     LVQFTEWMLELNKSGNEQQWLNQAPFSFDLSVMAIYPCLASGGTLNLVDKNMINKPKL
                     LNEMLTATPINIWVSTPSFMEMCLLLPTLNEEQYGSLNEFFFCGEILPHRAAKALVNR
                     FPSATIYNTYGPTEATVAVTSIQITQEILDQYPTLPVGVERPGARLSTTDEGELVIEG
                     QSVSLGYLKNDQKTAEVFNFDDGIRTYHTGDKAKFENGQWFIQGRIDFQIKLNGYRME
                     LEEIETQLRQSEFVKEAIVVPVYKNDKVIHLIGAIVPTTEVTDNAEMTKNIKNDLKSR
                     LPEYMIPRKFEWMEQLPLTSNGKIDRKKIAEVING"
     gene            complement(21709..21861)
                     /locus_tag="ACGEXS_000399"
     CDS             complement(21709..21861)
                     /locus_tag="ACGEXS_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFF8011447.1"
                     /translation="MKSKSKQPPNKYVEAFKPYLLTLLYLAIFITLYLIYGSGDTHNN
                     FIYNEF"
     gene            complement(21909..22019)
                     /locus_tag="ACGEXS_000400"
     CDS             complement(21909..22019)
                     /locus_tag="ACGEXS_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011448.1"
                     /translation="MMTTNYYVESIKLKLNFIMNIDIMNCKKQILKRILY"
     gene            complement(22273..23232)
                     /locus_tag="ACGEXS_000401"
     CDS             complement(22273..23232)
                     /locus_tag="ACGEXS_000401"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - hydroxypyruvate reductase (NADH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFF8011449.1"
                     /translation="MVKIVVSRKIPDKFYQQLSKLGDVVMWQKSLVPMPKDQFVTALR
                     DADACFITLSEQIDAEILAQSPNLKVIANMAVGYDNIDVESATANNVVVTNTPNVLTE
                     TTAELGFTLMLAIARRIVEAEKYVEADAWQSWGPYLLSGKDVFNSTIGIYGMGDIGKA
                     FARRLQGFNTNILYHNRSRHKDAEADFNATYVSFETLLAESDFIICTAPLTKETHHKF
                     NAEAFEQMKNDAIFINIGRGQIVDETALIDALDNKEILACGLDVLANEPIDHTHPLMG
                     RDNVLITPHIGSASVTTRDNMIQLCINNIEAVMTNQVPHTPVN"
     gene            complement(23265..24044)
                     /locus_tag="ACGEXS_000402"
     CDS             complement(23265..24044)
                     /locus_tag="ACGEXS_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFF8011450.1"
                     /translation="MKQYKAYLIDLDGTMYMGTDEIDGAKQFIDYLNVKGIPHLYVTN
                     NSTKTPEQVTEKLREMHIDAKPEEVVTSALATADYISEQSPGASVYMLGGSGLNTALT
                     EAGLVIKNDEHVDYVVIGLDEQVTYEKLAIATLGVRNGATFISTNPDVSIPKERGLLP
                     GNGAITSVVSVSTGVSPQFIGKPEPIIMVKALEILGLDKSEVAMVGDLYDTDIMSGIN
                     VGMDTIHVQTGVSTLEDVQNKNVPPTYSFKDLNEAIAELEK"
     gene            complement(24044..24478)
                     /locus_tag="ACGEXS_000403"
     CDS             complement(24044..24478)
                     /locus_tag="ACGEXS_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HFF8011451.1"
                     /translation="MYFVDKDKLTQKLAYLQALTDDYHESKHNHYAFERIAQMLIESS
                     VDIGNMIIDAFILRDPGNYKDVIDILELENVITKETQQAINKTVGIRKQFTYDYTALD
                     IKIIMPMFDDALPYYKQFITEVTTFLHQENVPVTAFGKGENQ"
     gene            24576..24842
                     /locus_tag="ACGEXS_000404"
     CDS             24576..24842
                     /locus_tag="ACGEXS_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3055 domain-containing protein"
                     /protein_id="HFF8011452.1"
                     /translation="MIDMYLYDDNEESQVQFVGFVGEHSRYDLMLVHTNRHYGKTLVL
                     NMQTNKFGIIGTDDLKEEGYIAHILGVNAEEGDEITEYLNEVIH"
     gene            complement(24921..25304)
                     /locus_tag="ACGEXS_000405"
     CDS             complement(24921..25304)
                     /locus_tag="ACGEXS_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFF8011453.1"
                     /translation="MIKVDQHYFELIENYRECFNEEQFIARYSDILDKYDYIVGDYGY
                     DQLRLKGFYKDSNKKAEMSKRFSNIQDYIFEYCNFGCPYFVLRHLSKQEVKKLIEEVH
                     PSDVIDDDNKLQDVKIKPTIQDTEH"
     gene            complement(25434..26351)
                     /gene="lipA"
                     /locus_tag="ACGEXS_000406"
     CDS             complement(25434..26351)
                     /gene="lipA"
                     /locus_tag="ACGEXS_000406"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFF8011454.1"
                     /translation="MATKNEEILRKPDWLKIKLNTNENYTGLKKMMREKNLNTVCEEA
                     KCPNIHECWGARRTATFMILGAVCTRACRFCAVKTGLPNELDLNEPERVAESVELMNL
                     KHVVITAVARDDLRDAGSNVYAETVRKVRERNPFTTIEILPSDMGGDYDALETLMASR
                     PDILNHNIETVRRLTPRVRARATYDRTLEFLRRSKELQPDIPTKSSIMVGLGETIEEI
                     YETMDDLRANDVDILTIGQYLQPSRKHLKVQKYYTPLEFGKLRKVAMDKGFKHCQAGP
                     LVRSSYHADEQVNEAAKEKQRQGEAQLNS"
     gene            complement(26435..27754)
                     /locus_tag="ACGEXS_000407"
     CDS             complement(26435..27754)
                     /locus_tag="ACGEXS_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFF8011455.1"
                     /translation="MRLTIYHTNDIHSHLHEYERIKAYMAEHRPRLNHPSLYVDLGDH
                     VDLSAPITEATLGKKNVALLNEAKCDVATIGNNEGMTISHEALNHLYDEAKFIVTCSN
                     VIDESGHLPNNIVSSYIKDIDGVKILFVAATAPFTPFYRALNWIVTDPLESIKEEIEL
                     QRGKFDVLIVLSHCGIFFDETLCQELPEIDVIFGSHTHHYFEHGEINNGVLMAAAGKY
                     GNYLGEVNLTFEAHKVVHKTAKIIPLETLPEVETSFEEEGKTLMSNSVIQHPVVLKRS
                     MNHITEAAYLLAQSVCEYTHAQCAIINAGLLVKDIVKDEVTEYDIHQMLPHPINMVRV
                     RLFGVKLKEIIAKSNKQEYMYEHAQGLGFRGNIFGGYILYNLGYIHSTGRYYLNGEEI
                     EDDKEYVLGTIDMYTFGRYFPTLKELPKEYLMPEFLRDIFKEKLLEY"
     gene            complement(27781..28608)
                     /locus_tag="ACGEXS_000408"
     CDS             complement(27781..28608)
                     /locus_tag="ACGEXS_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFF8011456.1"
                     /translation="MLLTITLLVLIGGLSAIIGSIVGIGGGIIIVPTMVYLGVEHGLL
                     HNITTQVAIGTSSVILIVTGLSSSLGYLKTKQVDIKNGSIFLFGLLPGSLLGSFISRY
                     LTFESFNLYFGIFLIFVAILLMVRNKIKPFKIFDKPKYEKTYVDAKGKTYHYSVPPLF
                     AFITTFLIGILTGLFGIGGGALMTPLMLIVFRFPPHVAVGTSMMMIFFSSVMSSIGHI
                     AQGHVAWGYAIILIISSYFGAKIGVKVNQSIKSDTVVTLLRTVMLLMGIYLIIRALI"
     gene            complement(28621..29469)
                     /locus_tag="ACGEXS_000409"
     CDS             complement(28621..29469)
                     /locus_tag="ACGEXS_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFF8011457.1"
                     /translation="MINIGLTGWGDHYSLYEDLERQTDKLKTYAGHFPVVELDATYYA
                     IQPERNILKWIKETPDTFEFVVKIHQALTLHADYKTFADTRQELFDQFKNMLEPLHTQ
                     KKLAMVLVQFPPWFDCNAQNIKYILYVRQQLQAFPMCVEFRHQSWFSDAFKEQTLAFL
                     TEHQIIHAVVDEPQVKDGSVPLVNRITNEIAFVRYHGRNHYGWTKKDMSDQEWRDVRY
                     LYDYNEQELIDLAQKAQILAQKAKKVYVIFNNNSGGHAANNAKTYQRLLNIEYEGLAP
                     QQLKLF"
     gene            complement(29517..29615)
                     /locus_tag="ACGEXS_000410"
     CDS             complement(29517..29615)
                     /locus_tag="ACGEXS_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011458.1"
                     /translation="MRFYFTNLIKRKHQAEDRRKILIKQKHQTKVR"
     gene            complement(29682..30749)
                     /locus_tag="ACGEXS_000411"
     CDS             complement(29682..30749)
                     /locus_tag="ACGEXS_000411"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HFF8011459.1"
                     /translation="MWNKNRLTQMLSIEYPIIQAGMAGSTTPKLVASVSNSGGLGTIG
                     AGYFNTQQLEDEIDYVRQLTSNSFGVNVFVPSQQSYTSSQIENMNAWLKPYRRALHLE
                     EPVVKITEEQQFKCHIDTIIKKQVPVCCFTFGIPSEQIISRLKAANVKLIGTATSVDE
                     AIANEKAGMDAIVAQGSEAGGHRGSFLKPKNQLPMVGTISLVPQIVDVVSIPVIAAGG
                     IMDGRGVLASIVLGAEGVQMGTAFLTSQDSNASELLRDAIINSKETDTVITKAFSGKL
                     ARGINNRFIEEMSQYEGDIPDYPIQNELTSSIRKAAANIGDKELIHMWSGQSPRLATT
                     HPANTIMSNIINQINQIMQYK"
     gene            complement(30763..31803)
                     /locus_tag="ACGEXS_000412"
     CDS             complement(30763..31803)
                     /locus_tag="ACGEXS_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFF8011460.1"
                     /translation="MIIAIIILIFISFFFSGSETALTAANKTKFKTEADKGDKKAKGI
                     VKLLEKPSEFITTILIGNNVANILLPTLVTIMALRWGISVGIASAVLTVVIILISEVI
                     PKSVAATFPDKITRLVYPIINICVIVFRPITLLLNKLTDSINRSLSKGQPQEHQFSKE
                     EFKTMLAIAGHEGALNEIETSRLEGVINFENLKVKDVDTTPRINVTAFASNATYEEVY
                     ETVMNKPYTRYPVYEGDIDNIIGVFHSKYLLAWSNKKENQITNYSAKPLFVNEHNKAE
                     WVLRKMTISRKHLAIVLDEFGGTEAIVSHEDLIEELLGMEIEDEMDKKEKEKLSQQQI
                     QFQQRKNRNVSI"
BASE COUNT        10063 a         5683 c         4776 g        11325 t
ORIGIN      
        1 catttcagac cctttgtatg attttcggta cgatttatct gttcctttag tcgtttcgaa
       61 ttgctcgctt tgactgtttt atccagattg gtaagcgttc tttaagtgtt tgaaacccat
      121 atttttctgt ttctctgatt tcatctaata ctgattgttt ttccttctta cgctcataat
      181 ttttgaagta atcattatcc tttaatgata gattaagctt tccttcatca tctatagaca
      241 aaattttagc tttaacaatt tggccttctg atagaaattt cttcaaatta tgaacgtagt
      301 catccataat ttctgatata tgaatcagtc cttcagtatg attaggggtc tcaacaaacg
      361 caccgtatgg ttgaatacca gttacacgca ccttgatatg ttggccaatt ttgtagttat
      421 tcaacttaat aaccccttat ttcgtttact taacactatt atcatagcac aaatcgaaat
      481 ggtggactaa ttaaaacatc aaatcatacg aatattattt tataaatgaa taagaattct
      541 acaaacttgg atttacaaat aaaattagca acaaattcct atctaaatca atatgatatc
      601 cagctacttt gttttcaatt tatttactat ttgaatagca attctctgta aaaacataaa
      661 aagcaacctc aactattatc tttaaaatac acatctattt aaaaactgtg ctttcttgat
      721 aaatatattg agattgctca tcatattagt ttatttattt tcgttggaat atatactaat
      781 ccattcataa attagcttta tccgagttta aaatgttggt agttaattat gtttagatat
      841 tattcttcaa catcaataga ttcaattaca acatcatgaa gtggtttatc ttgtggtccc
      901 acttttgtat ttgcaatatc ttctaatgta gtttcaccat caatgatttg accgaatact
      961 gtatgttttt gatctaacca tggtgtacca cccttttcgc catatgcatc aacgattggt
     1021 tgaggccagc caccatctgc aagttgactt aacatatttt gaggtacttc tttcatttga
     1081 acaatgaaaa attgtgaacc attagtatta ggtcctgagt tagccattga taatgcgcca
     1141 tataagttaa atgcatttaa tgaaaattca tcttcaaaag caccgccata aatactttcg
     1201 ccacccatac cagtagctgt tggatcgcca ccttgaatca tgaagtcatt aatgacacgg
     1261 tggaatgtga ttccatcata ataaccattt tttgcatgtg tcacaaaatt ttcaactgtt
     1321 tttggtgcaa tatttggaaa taatttgaat gtcatgtcac ctttatttgt gtgcataacc
     1381 actttgattt caccttgttg tacttctttg tttaactgtg gatagttagc catttatatt
     1441 ctccattcat gttatgatac atatatatta acatactttt aagggaggaa aatagcatga
     1501 agctatatcg attttcacac aaaacaggta gctatggtgt cacgattgtt gaagaaaatg
     1561 gagacgatgt ccttgttaaa gttgaacaag ttatcaaaca cccgaaacaa ggtgatttac
     1621 ataaccctac tgaaactgaa ggtgtatttt tccatgaccg caaagcatta agccattttg
     1681 aaaaacgcta tacgaaacgt tcacaattac gcgacttcaa tgttgatgta ttgcgctatg
     1741 aagattcact tcaacaagca attacagatt tagaaactaa aattaaagct cagcaaacaa
     1801 aacatgctga aatgtcttta gctagtttag cacgcttaaa agaagattat tcacttcaat
     1861 acaaagaaaa ttttaaataa aaaatgacgt gtttcttctt atttaaatac ttctgaaaat
     1921 aaagcacaac aaggagtgtg tacgtttttc agaactatat cattcatgta taatgaataa
     1981 cataaatatt ttttaaagga ggacaacata gtgagtttgt tacatattgc ggtgatatta
     2041 ccgttaatat tcgcactcat tattcctatc ctgtatagat tttttaaacg tatacattta
     2101 ggatggttcg tattacctgt ccctatcgtc atatttattt atatgcttac attaattaaa
     2161 acgacgatgt caggtaatac tgtaatgaaa accttaaatt ggatgcctca ttttggtatg
     2221 aactttgatt tatatcttga tggcttaggt ttactcttta gtttgcttat ttcaggaatc
     2281 ggcagtttag tagtattata ttcaatcggc tacttaagta agtcagaaca actagggaat
     2341 ttttattgtt atttattact atttatgggt gcaatgttag gtgtcgtact ttcagataat
     2401 gttattattc tatatttatt ctgggagcta acgtcatttt ctagtttttt acttatttca
     2461 ttctggcgcg aacgacaagc atctatatat ggtgctcaaa aatcgctcat aatcacagta
     2521 tttggtggtc tatcattatt aggtggtatc atcttactcg ctataccaac acaatcattt
     2581 agcattcagt acatgattca acatgcttct gaaattcaaa atagcccatt cttcattttt
     2641 gcgatgattt taatcatgat tggtgctttt acaaaatctg ctcagtttcc attttatatt
     2701 tggctaccag atgcaatgga agcacctaca ccagtcagtg cttatcttca ttcagcaaca
     2761 atggttaaag cagggttata tttaatcgct cgaatgacac caatcttcgc agcatcgcaa
     2821 ggttgggtat ggactgtcac actcgttggt ttaattacat tattctgggc ttcgctaaac
     2881 gcaacaaaac aacaagactt gaaaggtata ctggcatttt caacagtatc ccaattaggt
     2941 atgattatgg cgatgcttgg aatcggtgcc attagttatc actatcaagg cgatgatagc
     3001 aaaatatatg ctgctgcatt tacggctgca atattccatt taatcaacca tgcaacattt
     3061 aaaggtgccc tatttatgat tacaggtgca gtggatcatt ctactggtac acgtgatgtt
     3121 aagaaattag gtggcttact aacaattatg cctatttcat ttacaatcac tgttattaca
     3181 gcattaagta tggctggtgt accaccgttc aatggtttct tatcaaaaga atcattttta
     3241 gaaacaacat ttacagcaag ccaagcaaat ttattcagtg ttgatacatt aggctaccta
     3301 ttcccaatca ttggtatagt gggcagtgtc ttcacatttg tatattcaat taaatttata
     3361 atgcatatat ttttcggaca atataaacct gaacaattac ctaaaaaagc acacgaagta
     3421 tcaatattaa tgcttttatc accagctatt ttagctacat tagtaattgt atttggttta
     3481 ttccctggca ttttaactaa ttcgattatt gaaccagcta catcttcgat taatcatacg
     3541 gtaattgatg atgttgaatt ccatatgttc catggattaa cgcctgcctt tttatcaaca
     3601 ttagtgattt atattttagg tatcttacta attgtgacat ttagttattg ggtgaaattg
     3661 ttgcaacgac aacctggtaa actaacattc aattattggt acaacagaag tgcaaatgtt
     3721 attccaaact actctgaaaa gatgacgaat agctatgtta ccgattattc acgtaataat
     3781 ttagttatca tttttggtgc attaatttta ttaacatttg tcactatatt ctcagtacct
     3841 ttcaacatta attttaaaga cgttagtccg attagaattt tcgaagtttg tatcgtaatt
     3901 ttattattaa gtgctgcctt tttaattctt ttcgctaaat caagattatt tagcatcatt
     3961 atgttaagtg cagttggata tgctgtgtct gtattgttta tattctttaa agcacccgac
     4021 ttagcattga cacagtttgt cgttgaatct atttcaacag cattgttctt attatgcttc
     4081 taccacttac caaatttaaa tcgttataat gaaaagcact cattccagct tactaatgct
     4141 ttaattgctg gtggtgttgg tttgtccgtc attattatcg gtttaattgc atacggaaat
     4201 agacattttg aatcgatatc taagttttat caagagcacg tttatgattt agctcacggt
     4261 aaaaacatgg taaacgtcat acttgttgat ttccgtggta tggatacttt attcgagtca
     4321 tctgtgcttg gtatcgcagg attagctgta tatacaatga ttaaattacg taaaaagcgt
     4381 caaacgcaag gtaacgaggt gaaaaatcat gaatagacaa caaaatgatc taattttgca
     4441 atttgctgca gttattatct ttttcatggt aatggtcttt ggtttttcac ttttcttagc
     4501 aggacattat acacctggtg gcggtttcgt cggcggatta ttattcgcaa gttcacttgt
     4561 tatcattacg attgcatttg atatagaaac aatgcgtaaa attttcccat tagattttaa
     4621 aatcttgata ggtatcggat tagttttttg tattgcgacg cctatcgcaa gttggttttt
     4681 aggcaaaaat ttctttacgc atgtcacatt tgatataccg ttatttatat tagaacctgt
     4741 tcatatgaca acagcagtat tctttgattt cggtgtttta tgtgcagtag ttggtactgt
     4801 catgacaatt attatttcga ttggagagaa cgaatagtgg aaattattat gatttttgtt
     4861 agtggtattc tcacagcaat tagtgtctat ctcgttttgt ctaaaagtct gatacgaatt
     4921 gttatgggaa ctacactatt aacacatgca gcaaatttat ttttaataac tatgggcgga
     4981 cttaaacatg gtactgttcc aatttatgaa gcgaacgtaa aaagctatgt tgatcctatc
     5041 ccgcaagcac ttattttaac agcaatcgtt atcgcctttg cgacaacagc ctttttctta
     5101 gtattagcat ttagaacata taaagaatta ggcacagata acgttgagag tatgaaagga
     5161 gttccagaag atgattgaaa gtaatatgct tgtgttaacg ctagttattc cagtaattac
     5221 agccatatta cttgtcttta ttggaaagcg tcctattata aagcgttatg tcgcactagg
     5281 cggtacatta ttaaccttgg tcgcggcaat tatcaattta gcaaatgttg ttaaacatgg
     5341 accaatacgc gtagagctag gttcatggaa agcaccatac agtattgtct ttgtattaga
     5401 catattcagt gccctactaa taattactag tattattatt acagctattg tgattttata
     5461 ttcataccaa acaattggta ttgaacgtga aagatattat tattattttt cagtactatt
     5521 tatgttaata ggtatcattg gtgcattcac aactggtgac attttcaacc tattcgtatt
     5581 tttcgaagta tttttaatgt catcttattt cttattagta attggttcta caaaaattca
     5641 actacaagaa acaataaaat atgttcttgt taacgttgta tcgtcatcct tcttcgtaat
     5701 gggtgtagcg attttatatt cagtagtagg aacattgaat ctagcagata taagcaataa
     5761 attagctaac ctttcagcgc atgacagtgg cttagtcaat atcgttttca tactatttat
     5821 ctttgttttt gctacaaaag ctggcgtatt cccaatgttt gtttggttac ctagtgcata
     5881 ctatgcacca ccaattccaa tcattgcatt ctttggtgct ttgttaacaa aagttggtgt
     5941 ttatgcaatc gctagaacat tgagcctatt ctttagtgat aatgtaagct tttcacatta
     6001 tgtcatttta ttcttagcac tattaacgat catctttggt tgtgtcggtg ctgttgcata
     6061 tgccaacatt aaaaaaatca tcctatacaa tgtcatgatt gctgtaggtg tgatcttagt
     6121 cggcattgca atgatgacag agtcaggtat gattggggct atttactata cattgcacga
     6181 catgttagta aaacttgcat tattcttgct tatcggaatt atgattaaaa ttacaggcac
     6241 tgctgattta agacaatttg gtggcttaat taaacgatat cctgtacttg gttggtcatt
     6301 ctttatcgca gcattaagtt tagctggcat tcccccactc agtggctttt atggaaaatt
     6361 ttttattgtc caatcaacat ttgaaagagg attttattta agtggtgtca tcgtcttatt
     6421 atcaagttta gtcgtattat attctgtcat tcgcatattc ctacaaggct ttttcggtca
     6481 accaaaagga tatgatctta ataacaaagt cgacgtcaaa tatttaacta cgattgcaat
     6541 cgttgcagtt gtgataactg ttttatatgg attgtcagca gattacttat atccaatggt
     6601 aaaagctggt gcagaaacat tctacaatcc atcaacctac gttaaagctg tattaggagg
     6661 taaataacat ggctgtacaa ttagtattaa actttattat cgcagtgttt tggttgtttg
     6721 tgacaaatag ttacacaaca aataactttg tgttaggttt catctttggc ctagttcttg
     6781 tttacctctt acacagagtg ttaccaggta gattttatgt catcacgcta tatcgtatta
     6841 ttaaattagt tattattttc ttaatcgaac tgattaaagc taattttgat gttttgaaaa
     6901 ttattattaa accttcgatt aaaaatgaac caggattctt tgtttatcac acagatttaa
     6961 aaaaagattg gcaaattgtc ttgctgtcta atttaattac attaacacct ggtactgtag
     7021 tgcttggtgt aagtgacgat cgtacaaaaa tttatattca tgccatagat ttttcaacta
     7081 aagagcaaga agtagaaagt attaaaacat cattagagaa aattgtaaga gaggtgggcg
     7141 aaatataatg aatcataatg ttattatcgt tattgcatta atcatagttg tcatttctat
     7201 gttagctatg ctcattcgcg ttgtgctagg cccatcactt gccgatcgtg ttgtcgcatt
     7261 agatgcgatt ggtcttcaat taatggcagt tatagcatta ttcagtattt tattaaatat
     7321 taaatacatg attgtcgtta ttatgatgat tggtatatta gcttttttag gtactgcagt
     7381 attctctaaa tttatggaca aaggtaaggt gattgaacat gatcaaaatc atactgatta
     7441 gtcttgcact tatctttgtt atcatcggtg ctttaattag cgccctagca gctataggat
     7501 tattgagact tgaagatgta tattcacgtg cacatgctgc cggaaaagca tcaacattag
     7561 gtgcaatgtc attactattt ggtacgtttt tatattttat tgctactcaa ggttttgtaa
     7621 atatgcaatt aatcgttgcg attatctttg tattaattac aggtcctctt tcaagtcata
     7681 tgattatgaa agcagcatat aatattaaaa cgccttatac taaaaagact aaagtcgatg
     7741 aaatttcgga agacttaaaa gacacaaaat tgtagattat ataacaaaat catgaatata
     7801 aatcaacaac aaacagcagt aagatgattc caaattagga atgattttac tgctgttttc
     7861 ttttgacatt gttacctctt tttcaatgat tttttctttg actacagatt cgccctatct
     7921 acatatatct ctttaattta attgcctttc atgtcgttat gtattatgat aataataatt
     7981 ataaatcgta acgattacgt tttaaaaaga gagaggtttt attatgcatt ggacaattat
     8041 cggcggtggc atacagggaa ctgcaatcgc acaaaaacta ttatcaagcg gattaacaac
     8101 agaccgatta acaatcattg acccacacga aactttttgc caaaggttta actcatatac
     8161 aaatcgaata gaaatgcctt atttaagatc accgattgta catcacgtac atccacaacc
     8221 gttccatcta aaacaattcg ctaaacagca ccaatataca aatgcttttt atggtcctta
     8281 tcaacgacct gaattgacaa tgtttatgga tcatattgca catgcttcta aacaatatca
     8341 attagaggat tgcttggttc aaggtttagt tcaaacttta gataaacaag aagacaaatg
     8401 gcatatcaag ttagaagatg gacaaattat cactacagat tgcgtcgtta ttgcaatagg
     8461 cagtacaaat attccgttta tgcctgacat tttaaaagac aaacagaatg taaatcatat
     8521 cttcgagaaa gaacttgatc aagtagtata tgataagacc gatcatatcg ttggtagcgg
     8581 cattactgct gcacatcttg cacttaaatt gttaaatcat gataacgata aaaagattca
     8641 tttatggcta aataaagata ttgaaataca tgactttgat gctgatcctg gttggttagg
     8701 tccgaaaaat atgtcttcat ttttaagtac taaaagcatg cctgaaagaa atgccattgt
     8761 acaacgcgaa cgtcataaag gatcaatgcc tcacgaactg tacttacgcc ttaaaaaaca
     8821 tattaaaaat ggtcgtataa atgtgcataa aacacctatc actcaaatta gtggtggtgt
     8881 aattaacact gaaaatgatt ctgttccata tcaacagatt atggttgcaa ctggttttga
     8941 acaagatttt atgtcacaac cacttattaa gcaattaata caaaattatg atgcacctat
     9001 caacgaatgt aattaccctg ttatttccga aaaattagaa tggataccaa atctatttgt
     9061 cgcaggatgc tttgcagact tagaattagg accatttggt agaaatgtta tgggtggccg
     9121 taaagctgcc gaacgcattg aacaagcatt tctaaaacta caacaatata gcgcataaaa
     9181 aaactgctag ccgtagttat gaatatctca taacatttca gctagcagtt cttttatttt
     9241 aaaggtttaa tagcaactgt accacgcata actgtaatta attgttcttt gtcattctta
     9301 atttttatat cccatacatg tgtcgactta cctcgatgaa taatttcagc tgtcgcagta
     9361 acacgaccat ctttagcaga atgaatatgg ttagcattca tctctaaacc taatggaata
     9421 aatttggttg tatcaattaa attagcagat cctaatgaac atgctgtttc acctaaagca
     9481 atcgaagccc caccatgtaa atatccaaat ggttgtttta ctttatcagt aacaggcata
     9541 gaaataacaa ctaaaccatc ttcctggtga tctattgaca tctcaaatgt ctctaataaa
     9601 tgagtcatat tttcacctct ttatgcttat agtaccatag cagcaatagt accgaaaatc
     9661 attaaaggaa tattataaaa caagaagttt ggtacacatg tatcacgtat atgatcatgt
     9721 tggccatcaa catttaatcc cgcagttggt cctaatgttg aatcacttgc aggcgaacct
     9781 gagtcaccta atgcactcgc tgttccaatc aatgcgatta atgccattgt atctagtcca
     9841 attgacgctc caaaaggaat gaataatgat gcgataatag gaattgttgc aaatgatgat
     9901 ccaataccta aagtgacaat taaacctatc acatacatca tgataatgct aaataattta
     9961 ttatcaccag taatacttgt taaagtttta actaattcat ctatatcacc agtagcattc
    10021 attacaccag caaatccatt tgctgttaaa ataactacac caatataagc cataatttta
    10081 ataccttcaa caaacttagc atctaattca taccaattat atgcacgtga aataaagaat
    10141 acgagtaccc ctgccagtgc accaaaaatc attgaatctg taaatgtttg tactaaaaat
    10201 gtagctagta ttgctacaat tgttactatt aagatatatg gttttaactc tgtaacattg
    10261 tcactatctg aaattttacg tgtttcatat tcacgtggtt tacgatatac atataaaccg
    10321 ataagtaagc caacaatata ccccattgaa ggaataagca ttgctttcca aatcatatta
    10381 aactcaattg ggtgatttgc ctttgcaaag ccactttgaa taatttgctg gaaaatttga
    10441 ccgaatccat atggtaataa cacatacggg aaacataaac caaaaccgat aatcaaaccg
    10501 attaaacgtc tatctatttt taagtcatta aacagactta acaatggtgg gatgacaatt
    10561 ggaatgaatg caatatgtac aggaattaag ttttgactca tgatactcat agctaataat
    10621 gcaatgatta ttgtcacttt gactttaact cttgaccatc gactattttc agcgtgaatt
    10681 gcatttataa tttttcctac taaatagtct gtgataccac tgtatgaaat taatgctgca
    10741 aatccaccta ataaagcata gcttaatgct acctcagcac catcgactat atttttccca
    10801 aatacattta taactttttc aatgctcatg cctgaaatta agccaccaac tagcgcactg
    10861 ataaataagc ttataactac gtttaatcga cataaacata gcacaatcat taaaattact
    10921 gctattacta ctgcatttat catatgacaa ccatccttta tcactctatc gaactaaagc
    10981 gtttatagac tacatagtat cagtagcttt aacacctgtc aatagcaaac tataccaata
    11041 atctttattt tttagttaat tgtctttctt aaaataattt tagctttcat taaattaaac
    11101 aattttacaa gcttggaaca ccaatcaaaa tcctaagttc taaaatgcaa tattagtagt
    11161 cgttgactga atgaacatat gcttataata tttttttgca atgctagtca agttgattta
    11221 tgctcacaag gatatgcgat ttatattttc ttacaacaat gaaaatgcct gatacaatgc
    11281 gatcctttat actttcaaga gttaaattag aaatgtatat caagacacaa gttgtatcag
    11341 gcaaataatt aaattattgt tgttttaacc attgtactat cgtcggaatc ataaatcctg
    11401 ttggcccttt tggaccatta tatgaagctt tattagtcgt tgctggacct gcaatatcga
    11461 aatgaatgtg aggtgtttga ccactaaaat gtgttacaaa acttgccgca aatagcgctt
    11521 taccttgtcc attcgtatgg ttaactaaat cagcgatatc actgtgttta atacttgcac
    11581 gttcggttgc agtaatcggt aattcaaata ccatttcatc gacttcagaa cttatttgta
    11641 atatatcgtt taatatcact ttactattcg attcaaatgc agcagcttta tcatcgccta
    11701 gtgcaacaat tgctgcaccc gttaatgtag caaagtccat aatcacacta ggctgatatt
    11761 gatttgcata aaacacagca tctgcaagga ctaatctacc ttcagcgtct gtattcatta
    11821 cttctacagt ttcaccactt aatgctgtaa atacatcatc tggcttcatt gatgcttcat
    11881 ttatcatatt ttcagcacac gcaagcactc cgacaatatt tacaggcagt tgtaaacgac
    11941 tagccgcttc aatgatacca acgacattcg cagcgccaca catgtcaaac ttcattgtag
    12001 ccatgccatt cttcgtttta atactataac caccagaatc atacgttata cctttaccaa
    12061 ctaaggcaat tggtgcttca tctttgtctt tgccattata tgtgatggtt actaatctcg
    12121 gtttatgctt actaccttta cctactgctt gtaaaagtcc gaatccttca gaaactaaag
    12181 tgtcataatc tttaacatct actttgacct ttgtattttt aaaatgatta acaatatctt
    12241 ctgcaaatgt ttgtggtgtt aatacattcg gtggcatatt actaaagtct cttgccaaat
    12301 taatggattg gccaatactg ataccctcat gaataaaatc taattcaatt aatgattcac
    12361 taataaggtt taaattcgtc ttaaacggtg ccttcttact tgatttataa tgatcgaatt
    12421 catatgttgc acgctcactt tgaataccgc atgccattaa tacatctgat aactgatcat
    12481 attttgaaat aaatgaatcc ataagtaaat acgtatcttc aatgtgttct gactttatgt
    12541 attggaaaag atgtccccat attttcaaca tatcttgata actacgtgtc tttaagtttc
    12601 ctaaaccaac tgtaattaaa cgatatgttt gatcttgtac atcaaatgca gttgtataaa
    12661 tcttcccaac tttactacca ataatatgtt gatgttttag tctttcaagt gattctgtaa
    12721 tatcgatatg attaaaacta atgcgctcta actgatttaa atgttctggt ataccaataa
    12781 tcaatgtatt tatttcgttg cttagtgtgt tatttaattt aaaattcata atgtacctcc
    12841 ttagatttga tatgttactc acttcaaatt gtacaacaat aaagcccttc agtgacactg
    12901 aaggacttta taaatgaata atttaatttt atgtgtttag ctgaaacttt atttaaagcc
    12961 tagaatttac cttttttgaa tgctaaaccg ataccaccga ttttgaatac cgcacgtgta
    13021 tcaatcactt ttttcatgaa tgctgctttt ttaccagcga taggtttacc aaataccata
    13081 cctacaccgt catgtgaacc taaagaacaa acagttccac gatcaacgta ttcgaattct
    13141 tcagttgatt caccgtttaa gatgcgttta atgtttttag caacactttc accttgttgc
    13201 attgcaattt gtgctgtagt tggtaatgga cgttcttctc cagctgggat aaacgctgaa
    13261 cagtcaccaa taacaaaaat gttgtcgtaa ccattgattg ttaaatcttg ctttgtaacg
    13321 atacgtccac gtttaacgcc ttcaaatgat tcttccatta atttactacc acgtacacca
    13381 gctgcccata ctgaagtacc tgcatttaat tgttgttttt caccatctac ttcaactaca
    13441 aaaccttttt cgttacaagc aacgattggt gtagcaattt taaattcaac accgcggtct
    13501 tctaagtagc taactgcgtg gttaactaat tcttctgaga acattggtaa cattttaggt
    13561 gctgcttcaa cacaagtgat tttaacttta ttttgatcca caccatattt gctacataat
    13621 tcaggaattc tgtctgttaa ttcacctaag aattcaacac cagtgaatcc agcaccacca
    13681 actaagatag ataaatcgtt atcatctttt tcttttgatg ctgcatagtt agcaaatttg
    13741 tcttcgatat gacgtgataa ttcacgtgct gtgataacat tttcaatttg gaaagcatga
    13801 tctttcatac cttcgatgcc gaatgtttca ctaacgaaac ctaatgctac tactaaaata
    13861 tcaaagtcat aaataccttg atttgtttct acctttttag catcacggtc aatttttgtt
    13921 acttctgctt gaacaaagtt cactttgtct ttcttcaaga cactttccac aggatataat
    13981 acatcttcat agtttagtgt acctgctgat gcttcatgta accatgttgc ttcatagtga
    14041 tattcatttt tattaataag cgtaatttct gcttcttctg ttgatatcgc tttttgcaat
    14101 ttagttacag tttgtaaacc tgcataacca gcaccaagta caagtacttt tttacgatct
    14161 tgagccattt aatttcacct aagctttcat atttttttaa ccaaatgctg ataattacta
    14221 gtaacaactg gtcattacta cttttaccac aatgtatact agtttatcat cattttgtta
    14281 tttaagtctt ttaaattatg taaaaacctg tgatgttcac aaaaaagaca tttttaatcc
    14341 aatactaatt ctatagcttt tatgttatat tttcaagcaa gattcagtca gattgtgaaa
    14401 aaattaatta attttttcgt agcctgattt cgattaaaaa tgtatccgct atcacgatca
    14461 tttaaaatac tatttatagt cagattctta attttcaata agctttttaa taaaacaaaa
    14521 tatctttacg caaaaatagg acataatcaa atatgtcctt aatcgttatt gatatcaatt
    14581 tgtatcccaa aaattgggtt aaccattaaa aagttatttt attagcaatt ttcaggatta
    14641 cctgcaactt ttgcagttct aaatgaacta ccacagccac atgaagcaat tgcattagga
    14701 ttgtcgattt ggaaaccgcc acccattaat gattgcttaa aatcaatagt cgtaccattt
    14761 aatacgggtg catctttttt gtctactaat acttttaatc caaagtattc taagacttca
    14821 tcattttcac caggcgcttc ttctgcaccc ataccgtatg ttaaaccagt gcacccgcca
    14881 ccattcactt taatttttaa atagccatct ggcatttcat ttgctttaag catatctttt
    14941 acttcgtaag cagctgcttc tgttaatata actgttggca tgataactcc tccttaaaaa
    15001 atccaagttt cttttatatg tgcatatata ttttgtaata attcttccgg cgaatcacct
    15061 tcaacaatat caccatttac taaagcatac aacccggctg aacatatacc acaatgtgtc
    15121 aggcaaccat actctaacac atcgacatct gggtcatttt ccagttgatt aaaaacataa
    15181 tctccacctt ttgccatgtt agagagacaa aattctacga tcggattcat acttcacctt
    15241 cttatttcat ttgttacaat attatagcat tttaaaactg gtattttaac atgatgtgct
    15301 caattagcaa caactgatgt ttcttatccc agttatgtaa tagtgcctta gttagtacaa
    15361 atatttattt gtagtcattt tcaaaaaaat atataataag catataaata catatttact
    15421 tttttagggg ttattgatga atcaaagaca cttgcatttt ataactgttc attaacaaat
    15481 atatctatgc actttgatct tttcaaaatt aaagtaaata ttaacatata aaaggggtac
    15541 ttcattatga aaaacttagt tttgttaggc ggcggatatg gtaatatgcg tatcatgtca
    15601 cgcattttaa ctacttcttt accacaagat tatacagtca cattagttga tcgtatgcca
    15661 tttcatggat tgaaaccaga attttatgct ttagctgcgg gcacgaaatc agataaagat
    15721 gttcgtatga aattccctaa tcatccacaa gtgaatacag tttatggtga aattaacgac
    15781 atagatttag atgctcaaat tgtctcagtc ggtaattcta aaattgatta tgatgagcta
    15841 atcattggtt taggatgtga agataaatat cataacgttc caggagccga agaatataca
    15901 catagtattc aaacactctc aaaggctcgg gatactttcc atagtattag tgaactacca
    15961 gaaggtgcta aagtcggtat cgttggtgct ggattaagcg gcatagaact tgccagcgaa
    16021 ttaagagaaa gtagatcaga cttggaaata tatctttatg accgtgggcc gcgaatttta
    16081 agaaattttc cagaaaaatt aagtaagtat gttgcgaaat ggttcgccaa aaataatgtt
    16141 accgttgttc caaattcaaa tattaataaa gttgaacctg gtaaaatata taactgtgat
    16201 gaacctaaag atattgattt agttgtatgg acagcaggaa ttcaacctgt tgaagttgtt
    16261 gttaacttgc cgattgatat aaatagtaat ggacgcgtga tagttaacca gtatcatcaa
    16321 gtaccaacat atcgtaacgt ctatgtagtt ggtgattgtg ctgatttacc acatgcgcca
    16381 agtgctcagt tagccgaagt tcaaggtgat caaattgccg atgtgcttaa aaagcaatgg
    16441 ctaaatgaac cattacctga caaaatgccg gaactaaagg tacaaggtat cgttgggtca
    16501 ttaggagata agcaaggttt tgcatatatc atggaccgca ctgtaacagg acgactcgct
    16561 tctattttaa aatccggcgt actttggtta tataaatatc ataatggtta aaattaaaaa
    16621 gcaatgtcag ttcacactat tactgacatt gcttttagtt atgtgtatat tattcattca
    16681 caagcttttg gtctataaat cgagtgattt gctttgtttg tatataacca tctgctacat
    16741 attcatcgtt cattgtaatt aatggataaa atagttcatc ttgttctatt ctttcaataa
    16801 attgtaaatc atgatctgtt aagttgtcat tatcttttgt aatatctata tacgtatatt
    16861 taaatgatat atttgggtat tttcttttta atagcggctg tagccagtca taaatatctt
    16921 tcgatgttgg cgcattaacg caacttgcac atataacatc tgccccatat actaccacac
    16981 tcacgtgctc catatattta tcccccattg gtttgataga tttttattac actatctatt
    17041 ataatatact aataaagatt atcaaattca tctctcgaaa ggagacttgc ctgatgccta
    17101 ctgaagatac aacgatgttt gatcaagtag cagaagttat tgaacgtctt cgtccatttt
    17161 tattacgtga tggtggcgac tgctcattga ttgacgtgga agacggtatt gttaaattac
    17221 aattacatgg tgcatgtggt acatgcccaa gttctacaat cactcttaaa gctggtattg
    17281 agcgtgcatt acacgaagaa gtgcctggtg taatagaagt agaacaagta ttctaatata
    17341 aagatattaa gttaacagaa catattatga ataacaacaa agacaggaat actgaattat
    17401 ttataatcag ttattacctg tcttttttcg ctgtcaaatt ggactgatgg gaaagttatt
    17461 ttatactatt tatctgatta attattttat tattgccaca tatccaagca catattacta
    17521 ttctattcta tgaaatttaa aatataaaaa tagcacatac gctcaatcaa aatcgttgag
    17581 ttatgtgcta tttgtatttt aatttttagg tttatctact tcaggttgtg gttcaccttt
    17641 catatgtttc gcaatttgct catccatata aacccaacct ttccaaccga tgtgtacggc
    17701 atcactgata acatattttt cataatcttt atcagtcata tcgtaaattt taccaccatt
    17761 atctacaaca gtagaatgga tttttttata aactgcttga cgacgttctt tatcgatacc
    17821 aatgtggtca taccatacac cgtttgatgg aatacttaca tattgaacat ctgcaccagc
    17881 agcacgcatt gtttttacaa gtaattctaa atcttggaat tctggagaat taacattgaa
    17941 ttcgtagtca cgtctaactt tacgcttact ttcttgaatt aatttccagt attgatctct
    18001 aataccaaat ttattcgatg tagtatcagc tttaccaatt tcaactgctt tttgtttcat
    18061 ctcatcccaa gaagcgtctg gttttgtagc aggtttaaca tgttctaatg gagatttatc
    18121 cattgcaaac aatgatttaa tcgcttctat tttaatcaat tgattctctt taaaaccaga
    18181 aatataacta tctttagttt ctttagggtt tttagcataa gatttcaagt attctttatt
    18241 gtgtacatgt ggaaactgta ataaacgttg tgcataacga cgttttaatt cagtagacat
    18301 gtttttctgc tggaacattt gattaatttg agtttgagac atacgagcat caaagttttg
    18361 attcgttaaa ccatggtttg taaaccattg tggtgaaata ataaatgtta atttcttacc
    18421 ttttaattta tcatactgtg atgcaagttc aactgcgtta attaagtctg tagaaccacc
    18481 agcacctaat aagaatgctt ttttgttggc gttatgctta tttaatgcaa ttgcaggatt
    18541 aaatgggtca tctttaccta attcactaga gccgtatata ggataatact tgtttgattc
    18601 gtataactta tcttgaatga gtgtgccttt tagtacttga tctgtcaatg aagttctatt
    18661 atcttctaca gtcttttcat ttactaatcc tgtaaaccaa ctagcaggta ataatagaaa
    18721 gacaatgaat accgctccac taattaaaat gggtaaaaaa ggttttaatt tcatcgtaac
    18781 tcttctaatg cttcaacgat tttatttggt gttgcccact catctctatc aaagtccata
    18841 atagatactt cgatatcaag tttattttga atctctaata ataatccaac tgtttggaaa
    18901 gaatcaataa taccttcttc aaaaatttct acgtctggat tttcttttac aatatcattt
    18961 tctgctactt ctgctaataa atttaatact tgttctctaa attccataat taaattctcc
    19021 tttattatat aagtttacct gagaagatta aaaagccaaa tgttacaaag tggaatgtaa
    19081 tcacaatgct aagtgctgtt gtgaaaccat tttgccaacg tggcggatgt ttcttacgcc
    19141 aacgttcata atagccataa cctataaaca atgctgcatg gtataaacca taaacaatgt
    19201 aatacacttc gataccatgc caaattccca ttatgaagaa gttgattaag aatgccacgt
    19261 tagacattgc aaattgactc ttcaataatt ttttacgaga catgtagaat aaagatctca
    19321 tgtaaataca atctctgaac cagaatgata atgtcatatg ccatctattc cagaaatctt
    19381 taatattttt cgctttgaaa ggtttatcga agtttggtgg tgttttaata ccgaataaat
    19441 aactaaatgc tatcgcaaat aaactataac ctgcaaagtc aaagaataaa tataagctgt
    19501 atgcgtacat atataaccac aaattgacaa agccatgtaa gtctaattgt aacggcatga
    19561 ttgcatatgt gttaataaag taagcaacaa tatatttata caagaaacca agcataatca
    19621 tgtgaattgc ttttaatact aattcacgat attcattgcc tgttggtact tttttatcgt
    19681 ctttaacgaa acgtttgtaa cgatcgattg gtccagatga aatcgttggg aagaatgaaa
    19741 taaattgaat taatttccat actttaattt ctttgataga accatcacga atttccataa
    19801 ttaactgcac acttttgaat gtaacataag aaattcctaa gaaaccaaca aattcaatta
    19861 atttactttc atggaagtga atctgatgtc cacctaacca tgtactttgt aacactttca
    19921 caagtgctaa tggcaatatt gataaaacca ttacagttac aaattttgaa aatgaattat
    19981 tttttggttt tgaatgataa taaaacatta tcaataaaac ttgccatacg acgtaaataa
    20041 taaaactaat taattgaaca cttaaatact tttggtcaaa cagattatgt ttatcagaag
    20101 aaaagattaa cacaatcata aatgctgtaa ctacgccatt ataaatgtaa cttcgcttac
    20161 ctaaaaatcc aagtataatg actggtaata atgcaattaa agcaattaag aagaatgtaa
    20221 aatcaccata tggaatcatc cgttaattac ctctgcaatt ttctttctgt caattttacc
    20281 atttgaagtc aatggcaatt gttccatcca ttcaaacttt ctaggaatca tatactctgg
    20341 taagcgtgat ttcaagtcat ttttaatatt tttagtcatt tctgcattgt ccgtaacttc
    20401 agtcgttggc acaattgcac caattaaatg aataacttta tcatttttat atacaggtac
    20461 aacaatcgct tcttttacga actcagactg gcgtaattgt gtttcaattt cttctaattc
    20521 cattctgtag ccattcaatt tgatttggaa atcaatacga ccttgaatga accattgacc
    20581 attttcaaac ttcgctttat caccagtgtg atatgtacga ataccgtcat cgaaattaaa
    20641 tacttcagct gttttttggt cattttttaa gtatcctaaa cttacacttt gaccttcgat
    20701 aacaagttca ccttcatctg tagtagataa tcttgcgcct ggtctttcaa cgccaacagg
    20761 taatgtcgga tattgatcta agatttcttg tgtaatttga atacttgtaa ctgctaccgt
    20821 agcttcagtt ggaccatatg tgttgtaaat cgtcgcactt gggaaacggt ttactaacgc
    20881 ttttgctgct ctgtgaggta gaatttcacc acagaagaag aattcgttaa gactaccata
    20941 ttgttcttca ttaagcgttg gtaataataa acacatttcc ataaatgatg gtgttgatac
    21001 ccaaatgtta atcggtgttg ctgttagcat ttcatttaat aatttaggtt tattaatcat
    21061 gtttttatct acaagattta atgtaccgcc tgatgctaaa catggataaa tagccattac
    21121 agataaatca aatgaaaatg gcgcttggtt aagccattgt tgttcatttc ctgatttatt
    21181 aagttctaac atccactcag taaattgaac taaacttgca tattcaattt gaacaccttt
    21241 aggttcccca gtagaaccag atgtaaagat tgtgtatact gtgtcgttat ctttaatctg
    21301 actatcaaaa attactgggt cttgagatgt tttaatatct tctattgtaa atacttcgcc
    21361 ttctaaactt tcaaatgatt catcagtcgt attaaataca aactctggtt gaaccttgtt
    21421 aataatcatt ttaatacggt cttcaggaat tgaagtgtct acaggtacat atccacatcc
    21481 tgctttaatg gcaccaatca tcccaacaat catatatggt gacatgtgac cgaataaaat
    21541 catcggtttc ttactacctt gtaatcgatg tgctaattta ctagactcat ccattaactg
    21601 ttgataagtt aattcatcag ttgtgtgtct aacagcaatg ctttgtggat ttgcatccgc
    21661 aaacgcttgc agcttgttaa taatatctgt catattaagt ctccctcatt agaactcatt
    21721 ataaatgaag ttattgtgtg tgtcgccact gccataaatt aaatataaag taatgaatat
    21781 tgccaaatac aatagtgtta ataaatatgg tttgaatgct tcaacatatt tattaggtgg
    21841 ctgtttactt ttagatttca tattgcacct cttaaagttc ttagtaaaaa cgcctttata
    21901 aagaccgttc aatataaaat acgttttaaa atttgttttt tacaattcat tatatcgata
    21961 ttcataatga aattcaattt taattttata gattcaacat agtaattggt tgtcatcact
    22021 caattattgt aaatgataca ctttgttgag aacatcattc attttaaagg ttatttaaac
    22081 aataaacaat tacagtctat ataacaattt tgttatatac gtcaaaatca aataaactca
    22141 tcacattaat atgacgagtt tataatgtta ttgaattatc atcagcgcaa atatatacat
    22201 tcgcaagtca agcataacat atttaacaat tgctttgctt gttttaccaa tgattaaaaa
    22261 ccatacttat tttcaattta ctggagtatg tggtacctga tttgtcataa ccgcttctat
    22321 attattaata cataattgaa tcatattgtc ccgtgttgtt actgatgcgc taccaatgtg
    22381 tggtgtaatc agaacattat cacgtcccat taatggatgt gtatgatcaa tcggttcatt
    22441 tgctaataca tctaaaccac atgctaaaat ttctttattg tctagtgcat cgattaatgc
    22501 tgtttcatct acaatttgtc ctctaccgat attaataaaa attgcatcat ttttcatttg
    22561 ttcaaatgct tcagcattaa atttatgatg tgtttctttt gtaagtggcg ctgtacagat
    22621 gataaaatca ctttctgcta acaacgtttc aaaagaaaca tatgttgcat taaagtccgc
    22681 ctctgcatct ttatgtcttg atcgattatg ataaagaata ttagtattaa acccttgcaa
    22741 ccttcttgca aaagctttac caatatctcc cataccatat attccaatag ttgaattgaa
    22801 gacatcttta cctgacaata aataaggacc ccagctttgc catgcatctg cttctacata
    22861 tttttcagct tctacaatac ggcgtgctat agcaagcatt aatgtaaatc ctaattctgc
    22921 agttgtttca gtaagtacat ttggtgtatt cgtgacaacc acgttattcg ctgttgcact
    22981 ttcaacatcg atgttgtcat atcctacagc catattcgca attactttta aatttggtga
    23041 ttgcgctaaa atttctgcat cgatctgttc acttaatgta ataaaacaag catctgcgtc
    23101 acgaagtgct gtcacaaatt gatctttagg cataggcact aatgattttt gccacataac
    23161 aacgtcacca agtttactta attgttgata aaatttatct ggaattttcc tcgaaacaac
    23221 tatttttacc aaatcaattc acctacttta taaaatgact atatctattt ttctaattca
    23281 gctattgctt catttaaatc tttaaaagaa tacgttggtg gcacattttt attttgcaca
    23341 tcttctaacg tagatacacc tgtttgtaca tgaatcgtat ccatacctac gttaatacca
    23401 gacataatat cggtatcgta caaatcgcct accatagcaa cttcggattt atctaatcct
    23461 aaaatttcta atgctttaac cataataatc ggttctggtt taccaataaa ttgtggcgat
    23521 acacctgtcg atacacttac aacacttgta atagcaccat taccaggtaa taaaccacgc
    23581 tctttaggaa ttgatacatc aggatttgta gaaataaatg ttgcaccatt tcttacacct
    23641 aacgttgcaa tcgcaagctt ttcatatgta acttgttcgt caagtccaat aactacataa
    23701 tcaacatgct cgtcattttt aatgacaagt cccgcttcgg ttaacgcagt atttaaacca
    23761 ctcccaccta acatatatac tgatgctcct ggtgattgtt ctgaaatata atcagcagtg
    23821 gctaacgctg acgttacaac ctcttctggt ttagcatcaa tgtgcatttc acgtaatttt
    23881 tcagttactt gctcaggtgt ttttgttgaa ttattagtta cgtataaatg aggaatgcct
    23941 tttacattta aataatcgat gaattgtttt gctccatcaa tctcatctgt tcccatatac
    24001 attgtgccat ctaaatcgat taaatacgct ttatactgtt tcattattga ttttctcctt
    24061 taccaaaagc tgttactggt acattttctt gatgtaaaaa tgtcgttact tctgtaataa
    24121 attgtttgta ataaggtaat gcgtcatcaa acattggcat gataatctta atatctaagg
    24181 ctgtgtaatc atatgtaaat tgtttacgaa taccgacagt tttattaatc gcctgctgtg
    24241 tttctttagt aataacattt tctagttcta atatatcaat cacatcttta taattaccag
    24301 gatcccttaa aataaatgca tcgataatca tattccctat atctaccgat gattctatca
    24361 acatttgagc aatgcgttca aatgcataat gattgtgctt gctctcatga taatcatcag
    24421 ttaatgcttg taaataggct aatttctgag ttagtttatc tttgtctaca aaatacattc
    24481 cttgtcacct ctcacttata tattatcata attttgcagt atacttcatt tgtcgttata
    24541 ctaactttac tacttttaaa aggagtcgct acaaaatgat tgatatgtat ttatatgatg
    24601 acaacgaaga gagtcaagtt cagtttgttg gttttgttgg cgagcatagt cgatatgatt
    24661 tgatgttagt ccatacaaat agacattatg gtaagacact cgtacttaat atgcaaacaa
    24721 ataaattcgg tattatcggt actgatgatt taaaagaaga aggctatatc gctcatattt
    24781 taggtgtaaa tgctgaagaa ggcgatgaaa ttactgagta tttaaatgaa gtcattcatt
    24841 aaagataaaa acccctaact agattacagg tccatgcttg aattgcatga ttcccatttt
    24901 caatctagct aagggtttta ttaatgttca gtatcttgaa tggttggctt aatcttcaca
    24961 tcttgaagtt tattgtcgtc atctatcaca tcagacggat gaacttcttc gattaacttt
    25021 ttaacctctt gtttagacaa atgtcttaat acaaagtaag gacaaccaaa gttacaatat
    25081 tcaaatatgt aatcttgaat atttgaaaaa cgtttactca tctctgcttt tttattagaa
    25141 tctttgtaaa aaccttttaa tcgtaattga tcgtaaccat agtcaccaac tatgtaatca
    25201 tatttatcta aaatatctga atacctagca ataaattgtt cttcattaaa acattcgcga
    25261 taattttcta ttaattcaaa gtaatgttga tctactttta tcaaattctt cacctatcta
    25321 tgacaatgaa aggctcagtt tatactttta gtgtttcata aaaataatga ttacttctat
    25381 gacacgttcg ttttgtacaa actaagcctt tatgccttat taatggttaa atattaacta
    25441 tttaactgtg cctcaccttg gcgttgcttt tctttagcag cttcatttac ttgctcatcc
    25501 gcatgataag aactacgtac taaaggtcca gcttggcaat gtttaaaccc tttatccatt
    25561 gccacttttc ttaatttacc aaactctaaa ggcgtgtaat atttttgaac ctttaaatgt
    25621 ttacgtgaag gttgtaaata ttgaccaatc gttaaaatat ctacatcatt cgcacgtaaa
    25681 tcatccatcg tttcataaat ttcttctata gtttcaccta atccaaccat aatacttgat
    25741 ttagttggga tatccggttg taattctttt gaacgacgta aaaactctaa tgttctgtcg
    25801 taagtcgcac gcgcacgaac tctcggtgtt aagcgacgaa cagtttcaat attatggttt
    25861 aaaatgtcag gtcttgacgc cattaatgtt tctaacgcat catagtcccc gcccatatct
    25921 gatggtaaaa tttcaatcgt tgtaaatgga tttctttctc taactttacg tactgtctca
    25981 gcataaacat ttgaaccagc atctcttaaa tcatcacgcg caacagcagt gataacaacg
    26041 tgtttcaaat tcattaattc aactgattca gctacacgtt caggctcatt taaatcaagt
    26101 tcattaggta aacctgtctt aaccgcacaa aaacgacaag ctcttgtaca tacggcacct
    26161 aaaatcataa atgtcgctgt acgacgtgca ccccaacatt catgtatatt aggacattta
    26221 gcttcttcac atacagtatt aagatttttt tccctcatca tcttcttaag tcctgtatag
    26281 ttttcgttgg tatttaattt tattttcaac caatccggtt tacgtaatat ttcctcgttt
    26341 tttgtcgcca taacaacaca taccctcctg ttaagtgtat ctttaaacat tatatcgaaa
    26401 ttcatgacaa atgaaaatcc aataatctta ctttttaata ttccaataat ttttctttaa
    26461 atatatctct taaaaactct ggcattaaat actcttttgg taattctttc aatgttggga
    26521 aataacgacc gaacgtatac atatctatcg tacctagtac atattctttg tcgtcttcga
    26581 tttcttctcc attcagatag taacgccctg tagaatgaat gtaccctaaa ttataaagaa
    26641 tatatcctcc aaatatattc cctctgaaac ccaaaccttg tgcatgttca tacatatatt
    26701 cttgtttatt acttttagct ataatctctt ttaatttcac accaaaaagt ctaaccctta
    26761 ccatattaat cggatgcggt aacatttgat gaatgtcata ttctgtcact tcatctttta
    26821 caatatcttt aacgagtaag ccagcattga tgatggcaca ttgtgcatgt gtatactcac
    26881 aaacactttg agctaataag tatgcagctt cagttatgtg attcatacta cgcttaagca
    26941 ctactggatg ttgaattact gaattggaca ttaacgtttt tccttcttct tcaaatgaag
    27001 tttcaacttc aggtaatgtt tctaaaggaa taatctttgc agttttatgt actactttat
    27061 gtgcctcaaa agttaaatta acctctccaa gataattacc atactttcca gctgccgcca
    27121 tcagtacacc attattgatt tcaccatgtt caaaataatg atgcgtatga ctaccaaaaa
    27181 tgacatcaat ttcaggcaat tcttggcata atgtttcatc gaagaaaatg ccacaatgac
    27241 ttagcacgat taatacatca aatttacctc gttgaagttc aatttcttct tttatagatt
    27301 caagtggatc ggtaacaatc caatttagtg cacgataaaa tggggtaaaa ggtgctgtcg
    27361 ctgcaacgaa tagtattttc acaccgtcta tgtccttaat ataagaagaa acgatattat
    27421 ttggtaaatg acctgattca tctataacat tgctacatgt cactataaat tttgcttcgt
    27481 cgtaaaggtg atttaaagct tcgtgtgaaa tggtcatccc ttcattatta ccgattgttg
    27541 caacatcaca ttttgcttca tttagtaatg ccacattctt tttacctaaa gttgcttcag
    27601 ttataggtgc ggataaatct acatgatcac ctagatcaac atataaagaa ggatgattaa
    27661 gtcgtggccg atgttctgcc atatatgctt taatgcgttc gtattcatgt aaatgactat
    27721 gaatatcgtt cgtatgataa attgtaagcc tcaaattgac gtcctccttt taaagttgta
    27781 ttaaatcaac gcacgaataa ttaaatatat acccattaac aacattactg ttctcaataa
    27841 tgttactacc gtatctgact taattgattg attcactttg acaccgattt tcgcaccaaa
    27901 ataactagaa ataattaaaa tgattgcata accccaagct acgtgacctt gagcaatgtg
    27961 ccctatagaa ctcatgacac ttgaaaagaa aatcatcatc atacttgttc caacagctac
    28021 atgaggtgga aatctaaata caataagcat tagtggcgtc attagtgcgc cacctccaat
    28081 accaaataaa cctgtcaata taccaattaa aaacgttgta ataaaagcaa acaatggtgg
    28141 aacactataa tgatatgttt tacctttagc gtctacataa gtcttttcat acttgggttt
    28201 atcgaaaatt ttaaacggtt taatcttatt tcttaccatt aataaaatgg ctacgaaaat
    28261 taaaaagata ccaaaatata aattaaatga ctcaaatgtt aaatatctac taatgaagga
    28321 cccaagcaat gaacctggta atagtccaaa taaaaagatg gaaccatttt taatatcaac
    28381 ttgttttgtt tttaaatatc caagtgatga agaaagtcct gtcacaatta gaatgactga
    28441 agacgtccct atcgctactt gtgttgtaat attatgtagt aatccatgtt caacaccgag
    28501 gtaaaccatt gttggaacga taataatacc gcctccaatg cctacgatag accctataat
    28561 cgctgacaaa cctccgatta aaactaataa tgtaattgtt aataacatag tcgtcgcctc
    28621 ttaaaataat tttaattgtt gtggtgctaa cccttcatat tctatattca ataatcgctg
    28681 atatgttttg gcattatttg ctgcatgacc accagaatta ttgttaaata tgacgtaaac
    28741 tttcttagct ttttgtgcta atatttgtgc cttttgtgcc aagtctatta attcttgctc
    28801 attataatca tataaatagc gtacatcgcg ccattcttga tctgacatat ctttcttagt
    28861 ccaaccgtaa tgattacgtc catgataacg tacaaacgca atttcatttg tgattcgatt
    28921 gactaaaggt acactgccat ctttcacttg tggttcatca actactgcat gaatgatttg
    28981 atgttctgtt aaaaatgcca atgtttgttc tttaaatgca tcactaaacc atgattgatg
    29041 cctaaattct acacacattg gaaatgcttg taattgctgt cttacataca aaatatattt
    29101 gatattttgt gcattgcagt caaaccatgg cggaaattga accaatacca ttgctaattt
    29161 tttctgtgta tgtaagggct ctaacatatt cttaaattga tcaaatagtt cttgccttgt
    29221 atctgcaaat gttttgtagt ctgcatgcaa tgtgagtgct tgatgaattt tgaccacaaa
    29281 ttcaaatgta tcaggcgttt cttttatcca tttcaatata tttctttccg gttgtatcgc
    29341 atagtatgtc gcatctaatt cgacaaccgg aaaatgtcca gcatatgttt taagtttatc
    29401 ggtttggcgt tctaaatctt catataatga atagtgatca ccccaacctg ttaatccgat
    29461 gtttatcata tatatcacca atgtcatcat accatataac ttttatcata atcatttcag
    29521 cgaactttag tttgatgttt ttgcttgatt aaaatctttc ggcgatcttc agcttgatgt
    29581 tttcgtttga ttaaattggt aaaatagaaa cgcaatccac aaaaatggca agcactaaaa
    29641 taatgtttgg gggtgcttgt gcttttgtgg attgcggtcg attatttata ttgcatgatt
    29701 tgattaattt gattgattat attggacatg atggtgttgg cgggatgcgt tgttgctagt
    29761 cgcgggcttt gtccactcca catatgtatt aactctttgt cgccgatgtt tgctgcggct
    29821 tttcttatgc tacttgttag ctcattttgt attggataat ctgggatatc gccttcgtat
    29881 tgggacattt cttcgataaa cctattattg ataccgcgtg caagctttcc actaaacgct
    29941 tttgtaatga ctgtatctgt ttctttacta tttataattg catctcgcag tagttctgat
    30001 gcattactgt cttgtgatgt taaaaatgcg gtgcccattt gtaccccttc tgcacctaag
    30061 acaatacttg ccaaaactcc tctaccatcc ataattccac cagcggcaat gaccggaatt
    30121 gaaacgacat ctacaatttg tggcactaaa gatattgttc caaccatagg taattgattt
    30181 ttaggtttta aaaatgaacc acgatgtcca cctgcttcac taccttgagc aacgatagca
    30241 tccatacccg ctttttcatt cgcaatagct tcatcaacac ttgttgctgt acctataagt
    30301 ttgacattcg ctgctttcaa cctgcttata atctgttcgc ttggaattcc aaaagtaaaa
    30361 caacatacag gcacttgctt tttaattatc gtatcaatat gacacttaaa ttgttgttct
    30421 tcggtaattt ttacaaccgg ctcttctaaa tgtaatgcgc gtcgataagg ttttaaccat
    30481 gcattcatat tttcaatttg actactggta tatgattgtt gacttggtac aaagacattt
    30541 acgccaaaag aatttgacgt taattggcgt acataatcta tttcatcttc caattgctgc
    30601 gtattaaagt aacctgcgcc tattgtgcct aacccaccac tgttacttac tgatgcaact
    30661 aatttcggtg tcgtacttcc tgccatacct gcttgtataa ttggatattc aatacttaac
    30721 atttgagtaa gtcgattctt attccacata gctgttcgct ccttatatag atacgttgcg
    30781 atttttccgt tgttgaaatt gaatttgctg ttgagaaagt ttttcttttt cctttttatc
    30841 catctcatct tcaatttcca tacctaataa ttcttcaatt aagtcttcat gtgacactat
    30901 cgcttcagta ccaccaaatt cgtccaacac aattgctaaa tgttttctag aaatagtcat
    30961 cttacgtaat acccattcag ctttattgtg ttcattcaca aataatggct tagctgaata
    31021 gtttgtaatt tgattttctt ttttattact ccaagccaac agatatttag aatgaaacac
    31081 cccaataatg ttatcaatat ctccctcgta cactggatat ctagtgtatg gcttattcat
    31141 aaccgtttca taaacttctt cgtatgtcgc atttgaagca aatgccgtca cattaattct
    31201 aggtgttgta tctacatctt ttacttttaa attttcaaaa ttaatgacac cttccaacct
    31261 actcgtctca atttcattta aagcaccttc atgtccagca attgctaaca ttgttttaaa
    31321 ttcttctttt gaaaattgat gttcttgagg ttggccctta gataaacttc gattaatact
    31381 gtccgtcaac ttatttaaaa gtaatgtgat aggacggaac acaatgacac aaatattaat
    31441 aattggatat acaagccttg ttattttatc tggaaatgtt gcagcgacag acttgggaat
    31501 cacttcggag atcaaaatga taacaactgt taaaacagct gatgcaatac caacgctaat
    31561 cccccaacgt aaagccataa ttgtaacaag tgttggtaat aaaatattcg cgacattatt
    31621 cccaattaga atcgttgtaa taaactcact tggtttttca agtaacttta caatgccttt
    31681 tgctttttta tcacctttgt cagcttcagt tttaaatttt gttttattgg cagccgttaa
    31741 tgccgtctcg cttcctgaaa agaaaaacga aataaatatc aatataatta tggcaatgat
    31801 cacgcgtgta gtctccttat tgtcatatct tatttttatt gtagtta
//
LOCUS       DAXWFK010000011        30369 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.011, whole genome shotgun sequence.
ACCESSION   DAXWFK010000011 DAXWFK010000000
VERSION     DAXWFK010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 30369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30369
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.011"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            20..715
                     /locus_tag="ACGEXS_000413"
     CDS             20..715
                     /locus_tag="ACGEXS_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8011461.1"
                     /translation="MDVLTIEHLTKKIGNKTILEDVSFKLKRGQIVGLVGANGAGKTT
                     LMKVILGYSSFQSGNFNVINSKDSKSNIGALIENPGIYPFMSGYENLKLLNESKNTQD
                     IDKIVSQLHMDEYIHKKAKTYSLGMKQKLGIAIAFLNKPQFIILDEPMNGLDPKAVRD
                     VRELIVQKAQEGVTFLISSHILSELVKITNSILIINKGKIVTETSEEELKQFKDNDLE
                     NVLLEIIEREDQA"
     gene            717..1487
                     /locus_tag="ACGEXS_000414"
     CDS             717..1487
                     /locus_tag="ACGEXS_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8011462.1"
                     /translation="MGTLIKQECFKLFKKKSTFIAPIVFILLMVAQGYIATKYNEIFT
                     PQESFTSAYNGFSWFAFLLIIQASTIISMEFHYGTIKNLLYREYSRTTMIVSKIITLF
                     IISLIYFVITIIASIVIGSLFFNDLNIFESSGNQLSLLNQLLLVSLGTFVGVWLVLSL
                     TLLLSSATNSTGVAIAVGIVFYFASSILAVIQTALLEKIDWLKWNPINMMNIMLQTVE
                     KGFSKSTKLELHELFIGNIAYISIFLILVVFIFKKKNI"
     gene            1860..3824
                     /locus_tag="ACGEXS_000415"
     CDS             1860..3824
                     /locus_tag="ACGEXS_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HFF8011463.1"
                     /translation="MTQITEKELKKKYLDLLSQNFDTPEKLATEIINLESILELPKGT
                     EHFVSDLHGEYEAFQHVLRNGSGNVRAKINDIFKERLSTKELNDLTALVYYPEDKLKL
                     IKSDFQNCGQLNVWYITTIEHLIELIKYCSSKYTRSKLRKALPKQYVYIIEELLYKSN
                     EYQNKKSYYETLVNQVIELKQADDLIIGLAYSVQRLVVDHLHVVGDIYDRGPQPDKIM
                     DTLINYHSLDIQWGNHDVLWVGAYAGSKVCLANLLRICARYDNLDIIEDAYGINLRPL
                     LTLAEKYYDADNPAFKPKKRPDKHERLTQREESQITKIHQAIAMIQFKLEIPIIKRRP
                     NFEMEERLVLEKVNYDTNEITVYGNTYPLKDTCFQTVNRDNPAELLPEEEEVMNKLLL
                     SFQQSEKLRRHMSFLMRKGSLYLPYNGNLLIHGCIPVDENGEMESFEIDGHTYSGQEL
                     LDVFEYHVRKSFDEKENTDDLSTDLVWYLWTGKYSSLFGKRAMTTFERYFIADKASHK
                     EEKNPYYHLREDVNMVRKMLSDFGLNPDEGRIINGHTPVKEINGEDPIKADGKMLVID
                     GGFSKAYQSTTGIAGYTLLYNSFGMQLVAHQQFNAKEKILSEGIDELSIKRVVDKELQ
                     RKKIRDTNIGKDLQAQIDILKMLMHDRYLD"
     gene            4168..5247
                     /locus_tag="ACGEXS_000416"
     CDS             4168..5247
                     /locus_tag="ACGEXS_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011464.1"
                     /translation="MRILKESIIVAFAFVGVVVGAGFATGQEIFQFFTSHGAYSISGI
                     IVTGLLITLGGMVVMHTGHHLKSRNHSDSINYFLYPSIARGFDIILTMFMLSLAIIMT
                     AGGASTIHQSFNLPYWLSALILVAFILATLFLKFDRLIAVLGGVTPFLIAIVIMIAVY
                     YFTTSHLDFTAANNDAQIHKQKSLSPGWWFDAINYASLQIAAAFSFLSVMGSKVKYRD
                     STLYGGLIGGLIITFLLMMINLGLISQFDKIKHVDLPTLKLATQMSPSIGIIMSVIMI
                     LVIYNTVVGLMYAFASRFSVPFSRRYFIIIITMAVITYISTFIGFISLIGKVFPIMGL
                     FVFILLIPVLYKGLIKRITGKSHID"
     gene            complement(5352..5945)
                     /locus_tag="ACGEXS_000417"
     CDS             complement(5352..5945)
                     /locus_tag="ACGEXS_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFF8011465.1"
                     /translation="MEHIFREGQNGAPTLILLHGTGGDEFDLLPLGEALNENYHLLSI
                     RGQVSENGMNRYFKRLGEGVYDEEDLAFRGQELLTFIKEAAERYDFDIEKAVLVGFSN
                     GSNIAINLMLRSEAPFKKALLYAPLYPVEVTSTKDLSDVSVLLSMGKHDPIVPLAASE
                     QVINLFNTRGAQVEEVWVKGHEITETGLTAGQQILGK"
     gene            complement(5967..6935)
                     /locus_tag="ACGEXS_000418"
     CDS             complement(5967..6935)
                     /locus_tag="ACGEXS_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009560906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFF8011466.1"
                     /translation="MINNHELLGIHHVTAMTDDAERNYKFFTEVLGMRLVKKTVNQDD
                     IYTYHTFFADDVGSAGTDMTFFDFPNITKGQAGTNSITRPSFRVPNDDALTYYEQRFD
                     EFGVKHEGIQELFGKKVLPFEEVDGQVYQLISDELNEGVAPGVPWKNGPVPVDKAIYG
                     LGPIEIKVSYFDDFKNILETVYGMTTIAHEDNVALLEVGEGGNGGQVILIKDDKGPAA
                     RQGYGEVHHVSFRVKDHDAIEAWATKYKEVGINNSGIVNRFYFEALYARVGHILIEIS
                     TDGPGFMEDEPYETLGEGLSLPPFLENKREYIESEVRPFNTKRQHG"
     gene            complement(7013..7447)
                     /locus_tag="ACGEXS_000419"
     CDS             complement(7013..7447)
                     /locus_tag="ACGEXS_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFF8011467.1"
                     /translation="MDRTKQSLNVFVGMNRALDTLEQITKEDVKRYGLNITEFAVLEL
                     LYNKGPQPIQRIRDRVLIASSSISYVVSQLEDKGWITREKDKDDKRVYMACLTEKGQS
                     QMADIFPKHAETLTKAFDVLTKDELTILQQAFKKLSAQSTEV"
     gene            7674..7958
                     /locus_tag="ACGEXS_000420"
     CDS             7674..7958
                     /locus_tag="ACGEXS_000420"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8011468.1"
                     /translation="MSNLEIKQGENKFYIGDDENNALAEITYRFVDNNEINIDHTGVS
                     DELGGQGVGKKLLKAVVEHARENNLKIIASCSFAKHMLEKEDSYQDVYLG"
     gene            8129..8935
                     /locus_tag="ACGEXS_000421"
     CDS             8129..8935
                     /locus_tag="ACGEXS_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFF8011469.1"
                     /translation="MAFHDKTATQVTNIVLNVRDLDLMTTFYKNILGLSVKSSDDNTT
                     VLSVGTGGHTLTLHLLEDGRQTSPREAGLFHIAFLLPTTEDLANFLYFVAQKNMGIGA
                     GDHLVSEALYFNDPEGNGIEVYRDRPSSSWEWQNGKVKMDTLEVDSQTLLTHRTDEGW
                     QGMPAKGMIGHLHLKTHDLDAAYQCYIEQLGFQHVSDFPRALFMSTNHYHHHIAANTW
                     QSNQARQNNSQSYGLTHVDIYQPDASTHTFTAPEGFDITVHSNTDLVPEK"
     gene            complement(9055..9726)
                     /locus_tag="ACGEXS_000422"
     CDS             complement(9055..9726)
                     /locus_tag="ACGEXS_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFF8011470.1"
                     /translation="MSNMNQTIMDAFHFRHATKQFDPQKKVSKEDFETILESGRLSPS
                     SLGLEPWKFVVIQDQALRDELKAHSWGAAKQLDTASHFVLIFARKNVTSRSPYVQHML
                     RDIKKYEAQTIPAVEQKFDAFQADFHISDNDQALYDWSSKQTYIALGNMMTTAALLGI
                     DSCPMEGFSLDTVTDILANKGILDTEEFGLSVMVAFGYRQQEPPKNKTRQAYEDVIEW
                     VGPKE"
     gene            9996..10988
                     /locus_tag="ACGEXS_000423"
     CDS             9996..10988
                     /locus_tag="ACGEXS_000423"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFF8011471.1"
                     /translation="MTKIMFFGTRDYEKEMALNWGKKNNVEVTTSKELLSSATVDQLK
                     DYDGVTTMQFGKLENDVYPKLESYGIKQIAQRTAGFDMYDLDLAKKHNIVISNVPSYS
                     PETIAEYSVSIALQLVRRFPDIERRVQAHDFTWQAEIMSKPVKNMTVAIIGTGRIGAA
                     TAKIYAGFGATITAYDAYPNKDLDFLTYKDSVKEAIKDADIISLHVPANKESYHLFDK
                     AMFDHVKKGAILVNAARGAVINTPDLIAAVNDGTLLGAAIDTYENEAAYFTNDWTNKD
                     IDDKTLLELIEHERILVTPHIAFFSDEAVQNLVEGGLNAALSVINTGTCETRLN"
     gene            complement(11237..12037)
                     /locus_tag="ACGEXS_000424"
     CDS             complement(11237..12037)
                     /locus_tag="ACGEXS_000424"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFF8011472.1"
                     /translation="MVKAIAVDMDGTFLDSKKTYDKLRFEAIFTELRNRDITFIAASG
                     NQYAKLKSIFGDRDMYFISENGAVIYNGNELYNYKSFNRQVFQQVVDYLNMKQSIDQL
                     VICGLKSAYILKHTSEAFKEDTRFYYHQLKEIDSLQQLPEDDYVKIAFNINRETHPNV
                     DEEVATQFSNDIKLVSSGHDSIDIIMPNMTKGQALKRLLDKWEMSPSELMAFGDANND
                     KDMLAFAKHSYVMENSHDEELFNIASAVAPSNDKQGVLTIIEQEVLKK"
     gene            12358..12993
                     /locus_tag="ACGEXS_000425"
     CDS             12358..12993
                     /locus_tag="ACGEXS_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFF8011473.1"
                     /translation="MKLNNYSLKVKNKQLVDNCDLNFYIGQINHIVGKNGVGKSLLAK
                     DFLLNNSGNIPKSISQNVTLISSSSNIPNDITKDFLLSLLKSKFENNRQTFDKIYNIL
                     NIEAIPSNVLLKNLSDGQKQKLKLLSFLLEDHDLIILDEVTNALDKKTVNEIYEFLND
                     FIQSHQTKTIINITHNLSDLSALPGKYFIFKDLQIEEYQSKEEVINDYINL"
     gene            12999..13730
                     /locus_tag="ACGEXS_000426"
     CDS             12999..13730
                     /locus_tag="ACGEXS_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease"
                     /protein_id="HFF8011474.1"
                     /translation="MSTLKLEFKKSISNKIIYTLVVLFTFLFLLGYFLPIGIDKVKSL
                     SYSQFFFSSYTVATQLGFLLFSFVIAYFINKEYSNKNILFYKLIGDNIFTFFYKKVAV
                     LFFECLVFIILSITIISIIYSDFSHYLLLIILFSLVILQYILVVGTISMVSPNILISL
                     GISIVYWIGSVILVAINKNIFGIVAPFEASNTMYRAVEKILNNESTFMCPTEIINIVS
                     FFVLLFIVNTIVLLLSRKRWLKIGM"
     gene            complement(13890..14510)
                     /gene="srtA"
                     /locus_tag="ACGEXS_000427"
     CDS             complement(13890..14510)
                     /gene="srtA"
                     /locus_tag="ACGEXS_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase SrtA"
                     /protein_id="HFF8011475.1"
                     /translation="MKKWTNRLMTIAGVVLILVAAYLFAKPHIDNYLHDKDKDEKIEQ
                     YDKNVKEQASKDKKQQAKPQIPKDKSKVAGYIEIPDADIKEPVYPGPATPEQLNRGVS
                     FAEENESLDDQNISIAGHTFIDRPNYQFTNLKAAKKGSMVYFKVGNETRKYKMTSIRD
                     VKPTDVGVLDEQKGKDKQLTLITCDDYNEKTGVWEKRKIFVATEVK"
     gene            14704..15195
                     /locus_tag="ACGEXS_000428"
     CDS             14704..15195
                     /locus_tag="ACGEXS_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFF8011476.1"
                     /translation="MIRYAKKEDLNAILAIYNDAIINTTAVYTYEPQTIDERVAWFET
                     KQRKHEPIFVFEENGSVLGFATFGSFRPWPAYLYTIEHSIYVDASARGKGIASQLLHH
                     LIVEAKAKGYRALVAGIDASNKASIQLHQKFAFKHAGTLTNVGFKFNRWLDLAFYELD
                     LQD"
     gene            complement(15633..15803)
                     /locus_tag="ACGEXS_000429"
     CDS             complement(15633..15803)
                     /locus_tag="ACGEXS_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011477.1"
                     /translation="MLRLSIIFIAFIINTTITYGYTTEGTWVNLLFKSLSLSMIIVFM
                     FYYIRFVIEKKR"
     gene            complement(15865..15963)
                     /locus_tag="ACGEXS_000430"
     CDS             complement(15865..15963)
                     /locus_tag="ACGEXS_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011478.1"
                     /translation="MIKKKHLSGSLVPDRCFLIISEEVERFMENAY"
     gene            complement(15982..16881)
                     /gene="sdaAA"
                     /locus_tag="ACGEXS_000431"
     CDS             complement(15982..16881)
                     /gene="sdaAA"
                     /locus_tag="ACGEXS_000431"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFF8011479.1"
                     /translation="MFDSIRETIDYAVENNMSFADIMVKEEMELSGKSRDEVRAQMKQ
                     NLDVMRDAVIKGTTGDGVESVTGYTGHDAAKLRDYNETHHALSGYEMIDAVKGAIATN
                     EVNAAMGIICATPTAGSSGTIPGALFKLEKTHDLTEEQMIDFLFTSALFGRVVANNAS
                     VAGATGGCQAEVGSASAMAAAAAVAIFGGSPEASGHAMALAISNLLGLVCDPVAGLVE
                     IPCVMRNAIGSGNALISADLALAGIESRIPVDEVIEAMDKVGRNLPASLRETGLGGLA
                     GTPTGEAIKRKIFGTAEDMVKNN"
     gene            complement(16895..17575)
                     /gene="sdaAB"
                     /locus_tag="ACGEXS_000432"
     CDS             complement(16895..17575)
                     /gene="sdaAB"
                     /locus_tag="ACGEXS_000432"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFF8011480.1"
                     /translation="MAKSYDYQSAFDIIGPVMMGPSSSHTAGAVKIGNSARAILGDIP
                     KNIEIHYYESFAQTHQGHGTDVAIVGGAMGYSTFDNRIKSSLEIAADDGIKIDIIEEE
                     GDSIGQHPNCAYIKSSRNDGRYIEIIGISIGGGTIKIKGIHINGLEVDLNHGLPILVV
                     DGNMTKAQVNHFINDINDMKLDCKDEFIKIDEDKCLVVIPLNKAISESTLNTIKEKYS
                     DVNVSYIN"
     gene            complement(17578..18621)
                     /locus_tag="ACGEXS_000433"
     CDS             complement(17578..18621)
                     /locus_tag="ACGEXS_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFF8011481.1"
                     /translation="MDILLGVGTLVLVLVIMTLFLKYAPYGKQGLQALSGAACATFLP
                     QAFLSYAIGGVFDIKFLQDIGDLAGSLSGIAVGILTCLNLGVAPVFAVIVGLVLHDFK
                     LLPAFIAAYGVAFLIKWIEKKVPEGLDLIVVILFAPAIAFGLASIITPGVLATLKQIG
                     SAVTAVGDNNPYALAVILGLVIPVTGMTPLSSMVLTSLLGLTGVPMAIGALTCTGSSF
                     VNLILFRKLNIGGPSKAFAVCIEPLTQIDLIAQYPVQLYGTNALIGVVNACIVTFSGL
                     VIGVKGMATPIAGAIVLFGFNNAVTSIVTIATVIIVSIVLAYIIGTLINKFNLMNINF
                     KMPSKKNHIKESV"
     gene            19172..19375
                     /locus_tag="ACGEXS_000434"
     CDS             19172..19375
                     /locus_tag="ACGEXS_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011482.1"
                     /translation="MQLSHSVKVAISIYLALIFITFTSYLVIILYTSMTGHDVSHFVL
                     DSQHSHHGSLTQKHLSLPEISFK"
     gene            19558..20493
                     /locus_tag="ACGEXS_000435"
     CDS             19558..20493
                     /locus_tag="ACGEXS_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFF8011483.1"
                     /translation="MRYLANNHSALRLKGIILAIVGACLWGLGGTVSDFLFKYKNINV
                     DWYVTARLVVSGVFLLIMYKMMQPKRSIFSVFQDRRMLGKLLIFSILGMLVVQYAYMA
                     SINTGNAAIATLLQYIAPVYIIIWFVIRGVAKLTLFDVLAIIMTLLGTFLLLTNGSFS
                     NLVVNPASLFWGILAGVALAFYTIYPSDLLNRFGSILIVGWAMLISGVAMNLRHPIWH
                     IDITKWDISIILFLIFGIIGGTALAFYFFIDSLQYISAKETTLFGTVEPVVAVIASSL
                     WLHVAFKPFQIVGIILIMILILLLSLKRQPETLDE"
     gene            complement(20575..21543)
                     /locus_tag="ACGEXS_000436"
     CDS             complement(20575..21543)
                     /locus_tag="ACGEXS_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFF8011484.1"
                     /translation="MRKKWSTLAFGFLVAAYAHIRIKEKRSVKSYMLEQGIRLSRAKR
                     RFMYKEEAMKALEKMAPQTAGEYEGTNYQFKMPVKVDKHFGSTVYTVNDKQDKHQRVV
                     LYAHGGAWFQDPLKIHFEFIDELAETLNAKVIMPVYPKIPHQDYQATYVLFEKLYHDL
                     LNQVADSKQIVVMGDSAGGQIALSFAQLLKEKHIVQPGHIVLISPVLDATMQHPEIPD
                     YLKKDPMVGVDGSVFLAEQWAGDTPLDNYKVSPINGDLDGLGRITLTVGTKEVLYPDA
                     LNLSQLLSAKGIEHDFIPGYYQFHIYPVFPIPERRRFLYQVKNIIN"
     gene            complement(21676..22020)
                     /locus_tag="ACGEXS_000437"
     CDS             complement(21676..22020)
                     /locus_tag="ACGEXS_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFF8011485.1"
                     /translation="MVKQLNSVEAFREFIHQYPLAVVHVMRDQCSVCHAVLPQIEDLM
                     QSYPNVPLAVINQSQVEAIAGELNIFTVPVDLIFMNGKEMHRQGRFIDMQRFEHHLKQ
                     MNDSVNNDVDEH"
     gene            complement(22062..22472)
                     /locus_tag="ACGEXS_000438"
     CDS             complement(22062..22472)
                     /locus_tag="ACGEXS_000438"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFF8011486.1"
                     /translation="MTTEGLLVAEKEIEVNGYDIDAMGVVSNIVYIRWFEDLRTAFIN
                     QHMNYSTMINQGISPILMKTEAEYKVPVTIHDKPVGRIYLVKASKMKWVFQFEIVSAH
                     GVHCIGTQTGGFYRLSDKKITSVPQVFQDILATK"
     gene            complement(22852..24918)
                     /gene="ptsG"
                     /locus_tag="ACGEXS_000439"
     CDS             complement(22852..24918)
                     /gene="ptsG"
                     /locus_tag="ACGEXS_000439"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-specific PTS transporter subunit IIBC"
                     /protein_id="HFF8011487.1"
                     /translation="MFKKLFGQLQRIGKALMLPVAILPAAGILLAFGNAMHNEQLVEI
                     APWLKNDIIVMISSVMEAAGQVVFDNLPLLFAVGTALGLAGGDGVAALAALVGYLIMN
                     ATMGKVLHITIDDIFSYAKGAKELSQAAKEPAHALVLGIPTLQTGVFGGIIMGALAAW
                     CYNKFYNITLPPFLGFFAGKRFVPIVTSVVAIATGVLLSFAWPPIQDGLNSLSNFLLN
                     KNLTLTTFIFGIIERSLIPFGLHHIFYSPFWFEFGSYTNHAGELVRGDQRIWMAQLKD
                     GVPFTAGAFTTGKYPFMMFGLPAAAFAIYKNARPERKKVVGGLMLSAGLTAFLTGITE
                     PLEFSFLFVAPVLYGIHVLLAGTSFLVMHLLGVKIGMTFSGGFIDYILYGLLNWDRSH
                     ALLVIPVGIVYAIVYYFLFDFAIRKFKLKTPGREDEETEIRNSSVAKLPFDVLDAMGG
                     KENIKHLDACITRLRVEVVDKSKVDVAGIKALGASGVLEVGNNMQAIFGPKSDQIKHD
                     MAKIMSGEITKPSETTVTEEMSDEPVHVEALGTTDIYAPGIGQIIPLSEVPDQVFAGK
                     MMGDGVGFIPEKGEIVAPFDGTVKTIFPTKHAIGLESESGVEVLIHIGIDTVKLNGEG
                     FESLINVDEKVTQGQPLMKVNLAYLKAHAPSIVTPMIITNLENKELVIEDVQDADPGK
                     LIMTVK"
     gene            complement(25219..26958)
                     /locus_tag="ACGEXS_000440"
     CDS             complement(25219..26958)
                     /locus_tag="ACGEXS_000440"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFF8011488.1"
                     /translation="MAKIKANEALVKALQAWDIDHLYGIPGDSIDAVVDSLRTVRDQF
                     KFYHVRHEEVASLAAAGYTKLTGKIGVALSIGGPGLIHLLNGMYDAKMDNVPQLILSG
                     QTNSTALGTKAFQETNLQKLCEDVAVYNHQIEKGDNVFEIVNEAIRTAYEQKGVAVVI
                     CPNDLLTEKIKDTTNKPVDTSRPTVVSPKYKDIKKAVKLINKSKKPVMLIGVGAKHAK
                     DELREFIEMAKIPVIHSLPAKTILPDDHPYSIGNLGKIGTKTSYQTMQEADLLIMVGT
                     NYPYVDYLPKKNIKAIQIDTNPKNIGHRFNINVGIVGDSKIALHQLTENIKHVAERPF
                     LNKTLERKAVWDKWMEQDKNNNSKPLRPERLMASINKFIKDDAVISADVGTATVWSTR
                     YLNLGVNNKFIISSWLGTMGCGLPGAIASKIAYPNRQAIAIAGDGAFQMVMQDFATAV
                     QYDLPLTVFVLNNKQLAFIKYEQQAAGELEYAVDFSDMDHAKFAEAAGGKGYTIKSAS
                     EVDAIVEEALAQDVPTIVDVYVDPNAAPLPGKIVNEEALGYGKWAFRSITEDKHLDLD
                     QIPPISVAAKRFL"
     gene            complement(27005..27694)
                     /locus_tag="ACGEXS_000441"
     CDS             complement(27005..27694)
                     /locus_tag="ACGEXS_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFF8011489.1"
                     /translation="MNDYVQALLMILLTVVLYYFAKRLQQKYPNPFLNPALIASLGII
                     FVLLIFGISYNGYMKGGSWINHILNATVVCLAYPLYKNREKIKDNVSIIFASVLTGVM
                     LNFMLVFLTLKAFGYSKDVIVTLLPRSITAAVGIEVSHELGGTDTMTVLFIITTGLIG
                     SILGSMLLRFGRFESSIAKGLTYGNASHAFGTAKALEMDIESGAFSSIGMILTAVISS
                     VLIPVLILLFY"
     gene            complement(27687..28082)
                     /gene="cidA"
                     /locus_tag="ACGEXS_000442"
     CDS             complement(27687..28082)
                     /gene="cidA"
                     /locus_tag="ACGEXS_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like murein hydrolase modulator CidA"
                     /protein_id="HFF8011490.1"
                     /translation="MHKVQLIIKLLLQLGIIIVITYIGTEIQKIFHLPLAGSIVGLFL
                     FYLLLQFKIVPLTWVEDGANFLLKTMVFFFIPSVVGIMDVASEITLNYILFFAVIIIG
                     TCIVALSSGYIAEKMSVKHKHRKGVDAYE"
     gene            complement(28295..29173)
                     /locus_tag="ACGEXS_000443"
     CDS             complement(28295..29173)
                     /locus_tag="ACGEXS_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFF8011491.1"
                     /translation="MDIKHMKYFIEVVKQGGMTNASKSLYIAQPTISKAIKDIENEMG
                     TPLFDRSKRHLILTDAGQIFYEKSKEIVALYDYLPSEMERLNGLETGHINMGMSAVMN
                     MKILINILGAFHQQYPNVTYNLIENGGKTIEQQIINDEVDIGVTTLPVDHHIFDYTTL
                     DKEDLRLIVSREHRLAKYETVKLEDLAGEDFILFNKDFYLNDKIIENAKNVGFVPNTV
                     AQISQWHVIEDLVTNELGISILPTSISEQLNGDVKLLRIEDAHVHWELGVVWKKDKQL
                     SHATTKWIEFLKDRLG"
     gene            29340..29558
                     /locus_tag="ACGEXS_000444"
     CDS             29340..29558
                     /locus_tag="ACGEXS_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HFF8011492.1"
                     /translation="MSFYEFMQSFLGDDTPLGELVDWINQDSNFPREVKSHSEILSYF
                     RTNPCPESISVPVIKRALSVFNQFTNVQ"
     gene            29948..>30369
                     /locus_tag="ACGEXS_000445"
     CDS             29948..>30369
                     /locus_tag="ACGEXS_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HFF8011493.1"
                     /translation="MEYKKILIRLLIAFAVLFSADFTYQSVEQTHQSHAAVNYYSKNQ
                     CTWWAFKRRAQVGKPVSNRWGNAKNWYYNARKSKYATGRTPRKFAVMQSTAGYYGHVA
                     VVEQVYKNGSIKVSEYNFYRPLKYNTRVLSKKAARNFN"
BASE COUNT         9690 a         5554 c         4604 g        10521 t
ORIGIN      
        1 tagcaaagga gatagtgata tggatgtttt aacaatagaa catttaacaa agaagatagg
       61 caacaaaacg attctcgaag atgtatcatt taagctgaaa cgcggacaaa tagttggtct
      121 cgttggagcg aatggtgcag gtaaaacaac tttaatgaaa gttatattag gttactctag
      181 tttccaaagc gggaatttta atgttattaa cagcaaggac agcaaaagca atatcggcgc
      241 attgattgaa aatccaggaa tatatccttt tatgtctgga tatgaaaact tgaagttatt
      301 gaatgaatca aaaaacactc aagatatcga taaaattgtc tcacaacttc atatggatga
      361 atacattcat aaaaaagcta aaacgtattc tcttggtatg aaacaaaaat taggaattgc
      421 tatagcattt ttaaataaac ctcaattcat tatcttagat gaaccaatga atggcttaga
      481 tccaaaagct gtgcgagatg tacgtgaatt gattgtccaa aaagcgcaag aaggtgttac
      541 tttcttaatt tcgagtcata ttttaagtga attagttaaa atcacaaact ctatccttat
      601 tattaacaaa ggtaaaattg ttacagaaac atcggaagaa gaacttaaac aatttaaaga
      661 taatgattta gaaaatgtat tactagaaat catagaaagg gaggaccaag cataaaatgg
      721 gaactttaat taaacaagaa tgtttcaaat tatttaaaaa gaaatcaact tttatcgcac
      781 ctattgtctt tattctacta atggttgctc aaggttatat tgctacaaaa tacaatgaaa
      841 tttttacgcc acaggaatct ttcacatctg cttataatgg tttttcatgg tttgcatttt
      901 tattaattat tcaagcaagt acaatcattt caatggaatt tcattacggt acgattaaaa
      961 atttactcta tcgtgaatat tcaagaacaa ctatgattgt tagcaaaatc atcacattat
     1021 ttattatttc tttaatttat tttgttatta caattattgc ttcaattgtt attgggtctt
     1081 tattctttaa tgatttaaat atatttgaaa gtagcggtaa tcaattatct ttattgaatc
     1141 aattattatt agttagttta ggcacatttg ttggcgtttg gttagtttta agcttaacgt
     1201 tgctattatc atctgcaaca aattcaacgg gagtagccat tgctgtaggt attgtttttt
     1261 attttgcaag ttctatttta gcagttattc aaacggcact tttagaaaaa atagactggc
     1321 taaagtggaa tcctattaat atgatgaata ttatgcttca aacagttgaa aaaggcttta
     1381 gtaagtcgac aaaattagaa cttcatgaat tgtttattgg taatattgct tatatttcta
     1441 ttttcttaat acttgtagta tttattttca agaagaaaaa tatttagtaa cttaaagtat
     1501 taaatgtcta aatacacaca tattccatcg taattcaaaa tcattttcaa atcccttcac
     1561 ccaaataatg gtgcggggat tttttcatcc aaattttgga aattccacat tttacatatc
     1621 gtaatttttt aggaaactag tgaatataac aaatccctcc tctcattttt aaaatagata
     1681 tatcacttcc ccacttttac ttaactaaac tgcaacggtt cctaatacca aaatcctgcc
     1741 ctctattttt atcaattcaa gcatacttat tgaaaaatgt taacgttttc ttgataatca
     1801 ttgtaagcgc atttatttta taaactaacg tttgaaatat actacaggag tgacacgtaa
     1861 tgactcaaat tactgaaaaa gaattaaaaa agaagtattt agatttacta tcccaaaatt
     1921 ttgatactcc agaaaaactt gcaactgaaa ttatcaattt agaatcaatt ttagaattac
     1981 ctaaaggtac ggaacatttc gtcagcgatt tacatggtga atatgaagct ttccaacacg
     2041 tattacgcaa cggttctggg aacgtgcgag cgaaaatcaa tgatattttc aaagagagac
     2101 tttcaactaa ggagcttaat gacttaactg ctcttgtcta ctatccagaa gacaaattaa
     2161 aattgattaa aagtgatttc caaaattgcg gtcaacttaa tgtctggtat atcacaacaa
     2221 tcgaacattt aattgagtta attaaatatt gttcttcaaa atatacgcgt tcaaaactgc
     2281 gtaaagcatt gccaaaacaa tacgtttata ttattgagga actactgtat aaaagtaatg
     2341 aatatcaaaa taaaaaatca tattacgaaa cacttgttaa ccaagtaatt gaacttaaac
     2401 aggcagatga tttaattatt ggactcgctt attccgtaca acgcttagtc gtcgatcatt
     2461 tacacgttgt cggtgatatt tatgatcgtg gaccacaacc agataaaatt atggatacac
     2521 tgattaatta tcattcccta gatattcaat ggggtaatca tgatgtgctt tgggttggag
     2581 cctatgctgg gtcaaaagta tgcttagcaa acttacttcg aatttgtgca cgttatgata
     2641 atttagatat tatcgaagac gcttatggca ttaatttaag accactgctt actttagctg
     2701 aaaaatacta tgacgcagat aatcctgctt ttaagcctaa aaaaagacct gacaaacacg
     2761 aacgtttaac tcaacgtgaa gaaagtcaaa ttactaaaat tcatcaagct attgcgatga
     2821 ttcaattcaa gttagaaata ccaattatta aacgtcgtcc aaatttcgaa atggaagaac
     2881 gtcttgtgct tgaaaaggtt aattatgata caaatgaaat tacagtttat ggtaatacat
     2941 acccattgaa agacacatgt ttccaaactg tcaatcgtga taatccagca gaattactac
     3001 ctgaagaaga agaagtcatg aataaactat tattgtcatt ccaacaatct gaaaaattac
     3061 gtcgtcatat gtctttcttg atgcgtaaag gctctcttta cttaccatat aatggcaatt
     3121 tactcattca tggttgtatt ccagttgatg aaaatggtga gatggaatca tttgaaattg
     3181 atggtcatac ttacagcggc caagaattat tagatgtgtt tgagtatcat gtccgtaaat
     3241 catttgatga aaaagaaaat actgatgact tatcgacgga tttagtttgg tatttatgga
     3301 ctgggaaata ttcgtcacta tttggtaaac gtgccatgac tacgtttgag cgatacttta
     3361 ttgcagataa agcttctcat aaagaagaaa agaatccgta ctatcatctt cgtgaagatg
     3421 tgaatatggt tcgtaaaatg ctcagtgatt tcggattaaa tccagatgaa ggacgcatta
     3481 ttaatggtca cacaccagtg aaagaaatca atggcgaaga tcctatcaag gctgatggaa
     3541 agatgcttgt cattgatggt ggcttttcaa aagcttatca gtcaacaact ggcattgcag
     3601 gctatacact attgtataat tcattcggta tgcaactcgt tgcacaccaa caatttaacg
     3661 ctaaagaaaa aatactttcc gaaggtatcg atgaactttc tataaagcgc gtcgtagata
     3721 aagaattaca acgtaagaag attcgtgata ccaatattgg taaagatctt caagctcaaa
     3781 tagatatttt gaaaatgtta atgcatgatc gttacttaga ctaaagacga catttgatgc
     3841 atgtatcgtg cacttattta gtggtactgt gtttacgcat tggctacgca aacacgatga
     3901 taaataggcg acctagctac accgaaaaaa atcccctcac tattgcgagt agtgagggga
     3961 ttggtgtatc tatgctaatt cctgttaact gtattataac aactttacag caaaaaaatg
     4021 caaaatcaac taataaatta atgaaatgac cgacaccctc gcttctactc caatttaaca
     4081 aaatactata ttagtactat atttttcatt aacatgtatg ttacattttc tatattactt
     4141 aaaattacga agggacattt ttaaatcatg cgtatcttaa aagagtccat tattgtggca
     4201 tttgcctttg ttggtgttgt cgttggtgcc ggctttgcta ctggtcaaga aattttccag
     4261 tttttcacaa gtcatggcgc atatagcatt tcaggcatta ttgtaacagg actattgatt
     4321 actttaggtg gaatggttgt catgcataca ggtcatcatc taaagtccag aaatcattct
     4381 gattcaatta actatttctt atacccctct attgcaagag gttttgatat tattttaaca
     4441 atgtttatgt tgtctttagc tattattatg actgcaggtg gtgcgtcaac cattcatcaa
     4501 agtttcaact taccgtattg gctgagcgca ctcatattag tcgcctttat tttagcaaca
     4561 ctgtttctaa aattcgatcg tttaattgct gtgcttggcg gtgttacccc atttttaatt
     4621 gcgattgtca ttatgattgc ggtctactat ttcacaacaa gtcatcttga ttttactgcc
     4681 gctaataatg atgctcagat tcataagcag aaatcattat cacctggatg gtggtttgat
     4741 gcgattaact atgcaagctt gcaaattgct gctgccttca gcttcttatc agtgatgggt
     4801 agtaaagtta aatatcgtga ctcaacgtta tacgggggct tgattggcgg tttaatcatt
     4861 acatttttac tcatgatgat taatctaggt ttaatttctc aattcgataa aattaaacac
     4921 gtagatctac ctacattaaa attagcgaca caaatgtctc cgtcaattgg tattattatg
     4981 tctgtcatta tgatacttgt catctacaat actgttgttg gattaatgta tgcatttgcg
     5041 tcacgtttca gcgttccatt cagcagacgt tacttcatca ttattattac aatggctgtc
     5101 atcacttata ttagtacatt tatcggtttc atttcattaa ttggaaaagt attccctatt
     5161 atgggattgt tcgttttcat cttactcata cctgtactct ataaaggttt aattaagcgt
     5221 attaccggca aatctcatat cgattaaaat atctatacta ttgtaaaatt tagtacagca
     5281 cttcaaaatc aaaaaagcgg gcagatgaaa agtaaaaatc ttttccatct gtccgcttct
     5341 taatagaact gttatttccc aagtatttgt tgaccagccg ttaatccagt ttctgtaatt
     5401 tcatggccct tcacccaaac ttcttcgact tgtgccccac gtgtattaaa caagttaatg
     5461 acttgttcac ttgcagctaa tggcacaatt ggatcatgtt tccccataga aagcaacaca
     5521 ctgacatctg ataaatcctt tgttgacgtt acttcaactg ggtataacgg tgcatataac
     5581 aatgcttttt taaatggtgc ttctgaacgc aacattaagt taatcgctat atttgatcca
     5641 tttgaaaatc caacaagtac tgctttttca atatcaaaat cataacgttc agcagcttct
     5701 ttaatgaacg tcaacaattc ttgtccacga aatgccaaat cttcttcatc ataaacacct
     5761 tcaccaagac gtttgaaata acggttcatc ccattttctg aaacttgtcc tctaatactt
     5821 aacaagtgat aattttcatt caatgcttcg cctaacggta ataaatcgaa ctcatcacca
     5881 cctgtaccat gcaataatat tagtgttggc gcaccatttt gtccttctct aaaaatatgt
     5941 tccatcacaa atcctcctca ttccaattaa ccatgttgac gcttcgtatt aaaaggtcta
     6001 acttccgatt caatatattc tcttttattt tctaaaaatg gtggtaagga taacccttcg
     6061 cctaatgttt cataaggttc atcttccata aatcctggtc catctgttga aatttctatt
     6121 aaaatatgcc ccacacgtgc atataatgct tcaaaataga aacgattaac gatgcctgag
     6181 ttattaatac ctacctcttt atatttcgtt gcccacgctt ctattgcatc atgatctttc
     6241 acacgaaatg acacatgatg tacctcacca taaccttgac gtgctgctgg ccctttatca
     6301 tcttttatta agattacctg gccaccattg cctccttcgc caacttcaag taatgcgaca
     6361 ttatcttcat gcgcaatagt tgtcataccg taaacagtct ctaaaatatt tttaaagtcg
     6421 tcaaaataac ttactttaat ttcaatgggg cctaatccat aaatcgcttt atctactgga
     6481 accggtccat tcttccaagg tacaccaggt gctacccctt catttaactc atctgaaatt
     6541 aattgataca cttggccatc gacttcttca aatggcaaca cttttttacc aaataattct
     6601 tgaatacctt cgtgtttaac accaaactca tcaaagcgct gttcataata tgttaatgcg
     6661 tcatcgttag gcactctaaa agacggtctt gtaatggaat ttgttcctgc ctgccctttt
     6721 gtaatatttg gaaaatcaaa gaacgtcatg tctgtacctg ccgaacctac atcatctgca
     6781 aaaaaagtat gatacgtata aatatcatct tgattgactg tctttttaac taaacgcatg
     6841 cctagtactt ctgtaaaaaa tttataatta cgttctgcat catctgtcat tgcagtaaca
     6901 tggtgaatac ctagtaattc atgattattt atcattttat ataccctctt tatattttta
     6961 gtgcatttat ctcgaaatcg agatacttta atgtaaattt ttagtgcacg ccttacactt
     7021 ctgtagattg tgcacttagt ttcttaaacg cttgttgtaa gattgttaat tcatcctttg
     7081 ttaacacatc aaacgctttt gttaatgtct cagcatgctt agggaaaata tctgccattt
     7141 gactttgacc tttttcagtt aaacaagcca tatatacacg tttatcatct ttatcctttt
     7201 cacgtgtaat ccaacctttg tcctctaatt gacttacaac atatgaaatg ctgctacttg
     7261 caattaatac gcggtctcta atacgttgaa ttggttgcgg acctttatta taaagcaact
     7321 cgagcactgc aaattcagta atatttaagc catatcgctt tacgtcttct tttgtaattt
     7381 gctctaatgt gtctaacgcc ctattcattc cgacaaaaac attgagagat tgtttcgttc
     7441 gatccatatg atcaccaact tttttatttt atttcgattt cgagataaat tatatagctg
     7501 aatagattta atgtcaagat acgtgttttc atcttcattt caaactgtgc ttaataaaaa
     7561 cgactacata gatgtggcaa tgtcgtcaca tgactgtcat gcacctttga cttcgtttaa
     7621 gttaaaatag aatagggaaa tactataatc aatcatgata ggagttgata tgtatgagta
     7681 accttgaaat caaacaaggc gagaacaaat tctatattgg tgatgatgaa aataatgctt
     7741 tagctgaaat cacataccgt tttgtggata ataatgaaat taacattgat catacaggcg
     7801 tatctgatga acttggtggt caaggtgttg gcaaaaaact acttaaagca gttgttgaac
     7861 acgctcgaga aaataatttg aaaattattg cctcatgttc atttgccaaa catatgttag
     7921 aaaaagaaga ttcatatcaa gatgtatatc ttggttaaat aaattttgcg atggtgcaac
     7981 tgtaccatcg cttttttgtt cgcatcaata taaaattgca aaacgactag ttattttgca
     8041 tttcaaactt tatgctatca attcattttc ttttctggta tactttttat actaacttat
     8101 agaaaaatag aaaaagaggc gatgcataat ggcatttcac gataaaacag caacacaagt
     8161 gacaaacatt gtactgaacg taagagactt agacttaatg acaacatttt acaaaaacat
     8221 tttaggacta tccgttaaaa gttctgacga taatacaacc gtattatctg ttgggactgg
     8281 tggccatact ctaacgttac atttattaga agacggccgt cagacttccc cacgtgaagc
     8341 agggcttttt catatagcat ttttattacc aactactgag gatctagcta acttcttata
     8401 tttcgtggca caaaaaaata tgggcatcgg cgctggtgat catttagtaa gtgaagcttt
     8461 atatttcaac gaccccgaag gtaatggtat tgaagtctat cgcgatagac cttcatcttc
     8521 atgggaatgg caaaatggca aagttaaaat ggatacttta gaagttgata gccaaacctt
     8581 attaacacat cgtactgatg aaggttggca aggaatgcca gcaaaaggca tgattggaca
     8641 cttacattta aaaacacatg atttagacgc agcatatcaa tgttacattg aacaattagg
     8701 attccaacat gtgtctgatt ttccacgtgc gctatttatg tcaacgaatc attatcacca
     8761 tcatattgct gctaatactt ggcaatccaa tcaagcaaga caaaacaatt cacaaagtta
     8821 tggattaaca catgttgata tttatcaacc cgacgcctca acacatacat ttacggcacc
     8881 agaagggttt gacatcacag ttcattcaaa tactgacctt gttccagaaa aataatcatc
     8941 catactttat taatacaatt ttttcacgca aaagcctgcg attcatttat gtaatcgcag
     9001 gcttttttat tgctaacttt ttaattttat atatttagac atacggtatt ctatttattc
     9061 ttttggtcca acccattcaa taacatcttc ataagcttgg cgtgttttat ttttcggtgg
     9121 ctcttgttgt ctgtagccaa atgcgaccat cactgataaa ccaaattcct cagtatctaa
     9181 gatcccttta tttgctaaaa tgtctgtcac tgtatccaga ctaaaacctt ccatcggaca
     9241 tgaatcaata cctaacaatg cggctgtcgt catcatattg cctaatgcaa tatacgtttg
     9301 tttacttgac cagtcataca aggcttgatc attatcagaa atatggaaat ctgcttggaa
     9361 tgcatcgaat ttttgttcaa cagctggaat cgtttgtgcc tcatattttt taatatctct
     9421 taacatatgt tgtacatacg gtgatcttga cgttacattt ttacgcgcaa aaattagcac
     9481 aaaatggctc gctgtatcta attgttttgc tgcgccccaa ctgtgcgctt ttaattcatc
     9541 acgtaacgct tgatcttgaa tcacgacaaa cttccaaggt tctaacccaa gagaacttgg
     9601 agacaatcta cctgactcta atattgtttc aaaatcttct ttcgaaactt tcttttgtgg
     9661 atcgaattgc ttagtcgcat gtctgaaatg aaatgcatcc ataattgttt gattcatatt
     9721 gctcattgaa catagcctcc catttttata tttattgcta tacaaattat acttcatgtt
     9781 ttaaaaagac taaagtaatc cgtttgcaat tcactataac ttgtgcacat gttataagtt
     9841 ttctgcaata aaacacactt ttgaattttg ggaaatttag tgttataata tatttgtgat
     9901 atttttcaca attaaattgt ggcgtgattt taatgtgcat aatctttaat ttcactcatt
     9961 tacttttcaa tcttttaaaa gcgaggtttt taataatgac aaaaattatg ttctttggta
    10021 cgcgtgatta tgagaaagag atggcattaa attgggggaa aaagaataat gtcgaagtaa
    10081 ctacttctaa agagctatta tcaagtgcta cagtcgatca attaaaagat tacgatggcg
    10141 taactacaat gcaatttggt aagttagaaa atgacgttta tcctaaatta gaatcttacg
    10201 gtattaaaca aattgcacaa cgtactgctg gatttgatat gtatgattta gatttagcta
    10261 aaaaacacaa tattgtgata tctaacgttc ctagttattc acctgaaaca attgcagagt
    10321 attctgtttc tatcgcccta caattagtgc gtcgcttccc agatattgaa cgccgtgtac
    10381 aagcacatga ttttacttgg caagcagaaa tcatgtctaa accagttaaa aatatgactg
    10441 ttgcaattat tggtacgggt cgtatcggtg ctgctacagc taaaatatat gcaggatttg
    10501 gtgctacaat tacagcttat gacgcctatc ctaataaaga tttagacttt ttaacttata
    10561 aagatagtgt taaagaagct attaaagatg ccgatattat ttctttacat gttccagcga
    10621 acaaagaaag ctatcattta ttcgataaag caatgtttga tcatgtgaaa aaaggtgcaa
    10681 tcttagttaa cgcagcacgt ggtgcagtca tcaatacacc tgatttaatc gctgcagtga
    10741 acgatggtac tttgttaggt gctgcgattg atacttatga aaatgaagca gcatacttca
    10801 caaatgactg gactaataaa gacattgacg ataaaacatt attagagtta atcgaacatg
    10861 aaagaatttt agtaacacca catattgctt tcttctctga tgaagcagta caaaaccttg
    10921 ttgaaggtgg tttaaacgca gcattatctg tcattaacac tggtacatgt gaaacacgtt
    10981 taaattaata tatatattta aaagcaaagg cgcgccatgt gcctttgctt tttattttgt
    11041 atatctgtat caaaatcgat ttgattaaaa tccgctttat tttatcatct attcaaatga
    11101 ttttagtgcg cttattttac tatggcatta ttgcctaacc tgtttgagat atattaatat
    11161 ttgatgattc gtattcaaag ttttcattta acatgtattt agtatcatga tagctgcttc
    11221 attgatgata tactacctac tttttcaaca cttcttgttc gataatcgtt aatacaccct
    11281 gcttatcatt acttggagcg acagcagagg caatattgaa caattcttca tcatgactat
    11341 tttccatcac ataactatgc ttagcgaacg ctaacatatc tttatcatta ttcgcatctc
    11401 cgaaggccat gagctctgaa ggagacattt cccatttatc taacaatcgt tttaatgcct
    11461 gccctttagt catgtttggc ataataatat caatgctatc gtgcccactt gagacaagtt
    11521 taatatcatt gctgaattgc gttgctactt cttcgtcaac attcggatgc gtctcacgat
    11581 taatattaaa tgctattttg acataatcat cctcaggtaa ttgctgtaga ctgtcaattt
    11641 cttttaactg atgataataa aatctcgtat cttctttaaa cgcttcagaa gtatgtttta
    11701 aaatatacgc gcttttcaaa ccacagatga cgagttgatc aatactttgc ttcatattta
    11761 agtaatcgac aacctgttga aacacctgac gattaaagct tttataatta tataactcat
    11821 tgccattata aataactgca ccattttcag aaataaaata catatctcta tccccgaaaa
    11881 tagacttcaa cttcgcatat tgattgccac tcgcagcaat aaatgtaata tctctatttc
    11941 taagttcagt aaaaatcgct tcaaatctaa gtttatcata tgtctttttt gaatctaaaa
    12001 acgtcccgtc catatctacc gcaatagcct taaccatgat tatcctcctc attgctcttt
    12061 tagtcgtctt cattatatcc atctcatgac aagcaatgaa gtttgtcata ccacaatgaa
    12121 cctatattca cattttgaaa ttgctttcat atatgcaaaa cacattacac tggatatagc
    12181 attcagaggc gaacataaat ttcagaatat tgtaaaaata taacgatata tttatcattt
    12241 agacaaaata tagtgtgata tactaattta taacatctat acataatgtg aaagaatttt
    12301 taaaaatata attaaatatc ggtgtaataa atccctttat acataggagt tgtatacatg
    12361 aaactaaata actattcttt aaaagttaaa aacaaacaac tagttgacaa ttgtgattta
    12421 aatttctata ttggtcagat caatcacatt gttggtaaaa atggtgtagg aaaatcttta
    12481 ttagctaaag atttcttact aaataatagt ggaaatatcc ctaagtccat ttctcaaaat
    12541 gtaaccttaa tatcaagttc atcaaatatt cctaatgata taacaaaaga ttttttatta
    12601 tcattgttaa aatcaaaatt tgaaaacaat cgacaaacat tcgataagat ttataacata
    12661 ctaaacatcg aagcaatacc gtctaacgta ctactaaaaa acttgagtga tggtcaaaaa
    12721 caaaagctta aattattaag tttcttattg gaagatcatg atttaatcat attagatgaa
    12781 gttacaaacg ctttagacaa gaaaacagtt aatgaaattt atgaattttt aaatgatttt
    12841 attcaaagcc atcaaactaa aactattatc aatattacac ataatttatc cgatttaagt
    12901 gctttgccgg gtaaatactt tatttttaaa gaccttcaaa tagaagagta ccaatcaaaa
    12961 gaagaagtca taaatgatta cattaattta taattgaggt gtccactttg aaattagaat
    13021 ttaaaaaaag tatttccaat aaaattattt acacgcttgt tgtgctattc acattccttt
    13081 tcttacttgg gtatttttta ccaataggta tagacaaagt taaaagttta agctatagcc
    13141 agtttttctt cagttcatat actgttgcaa cgcaattagg gttcttacta ttttcatttg
    13201 ttattgcata ttttattaat aaagagtatt cgaataaaaa tatcctgttt tataaattga
    13261 ttggcgataa tatctttacc ttcttctata aaaaggtagc agtgttattt tttgaatgtt
    13321 tagtgttcat aattctaagt atcactatta tttcaattat atattcggat ttctcacatt
    13381 acttactatt aataatctta ttttcactcg ttatcttaca atatatctta gttgttggaa
    13441 cgattagtat ggtttcacct aacattttaa tcagcttagg tatcagcatt gtatattgga
    13501 ttggttcagt catcttagtt gccattaata aaaatatatt tggtatcgtt gctccatttg
    13561 aggcgagcaa tacgatgtat cgtgccgtag aaaaaatatt gaataacgag tcaacattta
    13621 tgtgtcctac tgaaattata aatatagtat cattcttcgt tcttctattt atagttaata
    13681 ctattgtctt attactaagt agaaagagat ggttgaaaat cggtatgtag tataaaaaag
    13741 gagcggtata cattgccgct tctttttatt atgtattttt acaatctcca ccaatgacac
    13801 gtcgtcattg ctacctcata ccaaacaaaa aacagtctcg caatcaagac tgttttccac
    13861 tcaatatatt catccattag cgtaatagat tatttgactt ctgtagctac aaagatttta
    13921 cgtttttccc aaacgcctgt cttttcattg taatcatcac aagtaattaa tgttaattgt
    13981 ttatctttac ctttttgttc atctagaact cctacatctg taggcttaac atctcttata
    14041 cttgtcattt tatacttacg tgtttcatta ccaactttaa agtacaccat actacctttt
    14101 ttggctgctt taagatttgt aaattgatag ttcggacggt caatgaaagt gtgtcctgca
    14161 attgaaatat tttgatcatc tagtgattca ttttcttctg caaagcttac acctctattt
    14221 aattgttcag gtgttgctgg tcctggatat actggttctt taatatcagc atctggaatt
    14281 tcaatatagc ctgccacttt cgatttatct ttcggaattt gaggtttagc ttgctgcttt
    14341 ttatctttac tcgcctgttc ttttacattt ttatcatatt gttcaatctt ttcatcttta
    14401 tctttatcgt gaagataatt atcgatatgt ggtttagcaa acaaatatgc tgccactagg
    14461 ataagtacca caccagcgat tgtcattaat cgatttgtcc attttttcat acgttaaggc
    14521 tccttttata catttcacaa tgcctgttaa catttcatat agtataccat taatttcaaa
    14581 atgactcata gcaattcatt ttatactata aaatttacat gtatactttt acgttagatt
    14641 tcattacaca tatttgcatt caaataacga aacgctttta ataattacta aggggaaatt
    14701 gatatgatta gatacgctaa aaaagaggat ttaaacgcta tattagcgat atacaatgat
    14761 gccattatca atactacagc tgtttatact tatgaaccac aaaccataga cgaacgtgtc
    14821 gcatggtttg aaacgaaaca acgtaagcat gagcctatct ttgtatttga ggaaaatgga
    14881 agtgtcttag ggtttgcgac gttcggttca tttagacctt ggccagcata cctatataca
    14941 atcgaacatt ctatttatgt cgatgcttca gctagaggaa aaggtattgc tagtcaatta
    15001 ctacaccatt taattgtgga agcaaaagct aaaggttatc gtgcgctagt tgcaggcatt
    15061 gatgcttcca acaaagcgag tattcagttg catcaaaagt ttgcttttaa gcatgccggc
    15121 acactgacca atgtaggttt taaatttaat agatggttag atttagcatt ttacgaatta
    15181 gatttacaag actagtaatg tttgaatcac ataatataaa caagacaacc atgttaattc
    15241 ccttaacata acaagccaac atataaaatt ttaaacttct caggggagtg ggacagaaat
    15301 gataaagagc cactaatgat ttattatgta gtggttctta cacattagcc acagctaatg
    15361 tgtacttaaa aataggaata catgagtaaa actcatgcat aagaaatact aatttctata
    15421 gaaaaagtat ttctttatcg tcgtcccacc ccaactcgca cattattgta agctgacttt
    15481 tcgtcagctt ctgtgttggg gcccaaaaag cttgttacaa gcgcattttc gttcagtcaa
    15541 ctactgccaa tataactttg tagagcatat tacattgatt tacattgtcc cttttattta
    15601 ttcttttcaa atactatccc catagctttg atttaacgct ttttctcaat aacaaaacga
    15661 atatagtaga acatgaaaac gataatcatg ctgagcgata aagatttaaa taatagattg
    15721 acccacgttc cctcagtcgt atatccatat gtaatcgttg tgttaatgat gaatgctata
    15781 aagatgattg atagtcttag catatcatca ctccttttaa gttattttag atatacgggg
    15841 gcgcttttgc aatcactatt ttgattagta tgcattttcc ataaatcttt caacttcttc
    15901 agagataatt aagaagcatc tatctggtac taatgatcca gacagatgct tcttttttat
    15961 caatatttta ttgttatctc attaattatt tttaaccata tcttcagctg tgccaaagat
    16021 tttacgttta attgcttcgc cagttggtgt gcctgctagt ccacccaatc cagtttcacg
    16081 taatgatgca ggaaggttac gaccaacctt atccattgct tcaataactt catcaacagg
    16141 gattctactt tcaatacctg ctaatgctaa atctgctgaa attaaagcgt tacccgaacc
    16201 aattgcattt ctcataacac aaggaatttc aacaagtccg gctactggat cacaaactaa
    16261 acctaataaa ttacttatcg ctaatgccat agcgtgcccg gatgcttctg gtgatcctcc
    16321 gaatatagct actgctgcag ctgcggccat tgcagatgct gaaccaactt cagcttggca
    16381 gccacctgtt gcaccagcta cacttgcatt gtttgctacg acacgcccaa acaatgctga
    16441 agtgaataag aaatcaatca tttgctcttc tgttaaatca tgtgtttttt ctaatttaaa
    16501 aagtgcaccg ggaatggtac ccgaggaacc agctgttggc gttgcacaaa taatacccat
    16561 cgcagcattg acttcatttg ttgcaatggc acctttgact gcgtcaatca tttcatatcc
    16621 agacaaagca tgatgtgttt cattataatc acgtagttta gcagcatcat gaccagtgta
    16681 gcccgttaca ctttcaaccc catcacctgt cgtccctttg attactgcgt ctcgcatgac
    16741 atctaaattt tgtttcattt gcgctcgcac ttcatcacgt gatttaccgc ttaattccat
    16801 ttcttcttta accatgatat ccgcaaatga catattattt tctacggcat aatctatagt
    16861 ctctctaatt gaatcaaaca tgtttattcc ccctctaatt tatataggaa acgtttacgt
    16921 cactgtattt ctctttaatt gtatttaatg ttgattctga gattgcttta tttaatggta
    16981 ttacaaccaa gcatttatct tcatctatct taataaattc atctttacag tctaatttca
    17041 tatcgttgat atcattgatg aaatgattta cttgtgcttt agtcatattt ccgtcaacaa
    17101 ctaaaattgg taatccatga tttaaatcta cttctagtcc atttatatga atacctttaa
    17161 ttttaattgt accaccaccg attgaaatac cgataatttc aatgtagcga ccatcattac
    17221 gagatgattt gatataagca cagtttggat gttgaccaat actatcgcct tcttcttcga
    17281 tgatatctat tttaatacca tcatcagctg caatttctaa tgaagattta attcggttat
    17341 caaatgttga atatcccatt gctccaccca caatagcgac atctgtacca tgtccttggt
    17401 gtgtttgagc aaatgattca taataatgta tttcaatatt tttaggaata tctcccaata
    17461 ttgcgcgtgc tgaattccca atctttactg caccagccgt atgagaactt gaagggccca
    17521 tcataactgg tccgataata tcgaaagcac tttgataatc atagctcttt gccataatta
    17581 aacactctcc ttaatatgat tctttttgct cggcatttta aagttgatat tcattaaatt
    17641 aaacttatta attagtgttc caataatata ggctaagaca atgctgacaa taatcactgt
    17701 tgcgattgtt acaattgatg tcactgcatt attaaagcca aacaatacga tggcgcctgc
    17761 aattggtgtt gccatacctt tgacacctat tactagtccg ctaaatgtca cgatacatgc
    17821 gttgacgacc ccaatcagtg catttgtacc atatagttgt actggatatt gcgctattaa
    17881 atcaatttgc gtcaatggct caatacaaac tgcaaatgct tttgacggtc caccaatgtt
    17941 taattttcgg aataaaataa ggttaacaaa tgagctacct gtacatgtta gtgctccaat
    18001 agccatagga acacctgtca gtcctaataa acttgttaat accattgaac ttagcggtgt
    18061 catacctgta acaggaatca ctagtcctaa aatgaccgct aatgcatatg gattgttatc
    18121 acctaccgca gtgacagcac tacctatttg ttttaatgtt gctagcacac caggtgtaat
    18181 gattgatgca agtccgaaag caattgctgg tgcaaataag atcaccacaa ttaagtccaa
    18241 gccttctgga actttctttt caatccattt aattaaaaaa gctacgccat aagctgcgat
    18301 gaatgctggt aataatttaa agtcatgtaa tactaaacca acaatgaccg caaatactgg
    18361 tgcaacgcct aagtttaagc acgttagaat acctactgcg ataccgctta aacttcctgc
    18421 taaatcccca atatcttgta gaaatttaat atcaaatacg ccaccaatag cataacttaa
    18481 gaatgcttgt ggtagaaatg tcgcacaagc tgcaccggat aatgcttgta gtccttgttt
    18541 accgtacggt gcatacttta aaaatagcgt catgatcact aaaaccaaga ctaatgtgcc
    18601 tacacctaac agaatatcca tttcccaaaa acctctctct atgtttattt tattttcaga
    18661 ccataaacat cgtacaccct taagaaaacg ttttcaactt ttatctgtta tcaaatcaaa
    18721 tatttaagtg aaatatttca tatttgtgaa agatttttaa aatggattgt ttcaaaaact
    18781 acttatgttg tcgttaatat ttactaatta actttttact ctatatttca aacagttgtg
    18841 tgacagtttt ttgataactt ttttacatct gaaagtaagt aatatttcta aaaactttta
    18901 atatttatac actttatctt tcgagctatt tattttttgt ttttgtgctg tgtttataaa
    18961 accgcacact atattcaaaa ataacttatt acacatatat ttgcaataag ataattaaag
    19021 taggatatta tttttagttt tctgatagga atgatgatag tctataggtt gaatctttac
    19081 ttttttttaa agctaaattt acatcaactt aacaatggtt ggttatactg aagatgaaaa
    19141 tatttagtat aacttagtgg aggcgataaa ggtgcaattg agtcattccg ttaaagttgc
    19201 aatttctatc tatttagcac ttatctttat aacgttcact tcttatttag tcattatttt
    19261 atatacgagt atgactggac atgatgtatc acatttcgtg ttagatagtc agcattctca
    19321 tcatggatct cttacgcaaa aacatttgag tcttcctgaa atctcattta aatagtttat
    19381 cctctgtgtt tcaacattca tttcccatat cgattcattt atctatcatc tagaccacta
    19441 catcttagat gattttttta ttttctcatt tcactctttc ttaaaagtcg atataatgaa
    19501 ttaaatcatt atcatacacc gacatatttt atgttgttgg tgttaagttt taaaggggtg
    19561 agatacttgg cgaataatca ttcagctttg cgtttaaaag gaattatact tgccattgtc
    19621 ggtgcttgtt tatggggatt aggtggtact gtttctgatt tcttgttcaa atataagaat
    19681 attaatgtcg attggtacgt cactgctcga cttgtagtca gtggtgtttt cttacttatt
    19741 atgtacaaaa tgatgcaacc caaacgttca atatttagcg tattccaaga tcgacgtatg
    19801 ttaggcaaat tacttatctt cagtatactg ggcatgttag tagtacaata tgcttatatg
    19861 gcatctatta atacaggtaa tgctgcgatt gcaacattac tacaatacat tgcgccagtt
    19921 tatattatta tttggtttgt cataagaggc gttgcaaaac taacattatt tgatgtgctt
    19981 gctattatca tgacactatt aggaacattt ttattattaa caaatggttc attttctaat
    20041 ttagtcgtca atcctgcaag tttattctgg ggtattttag ctggtgtagc actcgctttt
    20101 tacacaattt atccttcaga cctacttaac cgcttcggtt cgattctaat tgtcgggtgg
    20161 gcaatgctta tttctggtgt tgcgatgaat ttacgccatc caatttggca cattgatatc
    20221 actaaatggg acatatcaat tatattattt ttaatctttg gtattatcgg tggtaccgca
    20281 ctcgcatttt atttctttat cgacagttta caatacatat cagcgaaaga aacaacatta
    20341 ttcggaactg ttgaacctgt cgtagccgtt atcgcaagca gtctatggtt acatgtggca
    20401 ttcaaaccat ttcaaatcgt aggcatcatt cttattatga ttttaatttt attactatca
    20461 cttaaaagac aacctgaaac attagatgaa taagaaaact ctgataatca ctttagcaag
    20521 taactattat ttaacaacgt agttacctta taggtgatat cagagttttt tattttagtt
    20581 aataatattt ttcacttggt ataaaaagcg tcgtcgctct ggtatcggaa atactggata
    20641 aatatggaat tggtaatatc caggtataaa gtcatgttcg atacctttcg cactcaacaa
    20701 ttgcgataag ttcaaagcat ctgggtacag tacttctttt gtaccaacag ttaatgtaat
    20761 acgacctaaa ccatctaagt caccattaat tggtgatact ttgtagttat ctaaaggtgt
    20821 gtcccctgcc cattgttcag ctaagaacac actgccatcc acacctacca ttgggtcttt
    20881 ctttaagtag tcaggaattt caggatgctg catcgttgca tctaaaactg gtgaaattaa
    20941 tacaatatgt cctggttgca caatatgttt ttcttttaac aattgagcaa atgataaagc
    21001 aatttgaccg cccgcagagt cacccattac aacgatttgt ttagaatctg ctacttgatt
    21061 caataaatca tggtacaact tttcaaaaag cacatacgtc gcttgataat cttgatgcgg
    21121 aatcttcgga tatactggca tgatgacttt agcattgagt gtttctgcaa gttcatcaat
    21181 aaattcgaaa tgaattttga gtgggtcttg gaaccatgcg cctccatgtg catataatac
    21241 aacgcgttga tgcttatctt gtttatcgtt aacggtataa acggttgaac cgaagtgctt
    21301 atccactttt actggcatct taaactgata attggttccc tcatattcgc ctgctgtctg
    21361 tggcgccatt ttttctaatg ctttcatcgc ttcttcttta tacataaaac gacgcttagc
    21421 tctagataat cgtatacctt gttctaacat ataacttttc acactgcgtt tttctttaat
    21481 tctaatatgt gcgtatgctg caactaaaaa tccaaacgca agtgtagacc attttttacg
    21541 catttgtcgt cactcctctt tgatgatttc tactatagtt ctactgtacc atgctgattc
    21601 taattactac caatatttac tgggaaataa gttatgatgt ctacataatc ttagcaatgc
    21661 taatcatttg cgatattaat gctcatcgac atcgttattt acactatcat tcatttgctt
    21721 aagatgatgt tcaaaacgtt gcatatcgat aaaacgccct tgacgatgca tttcttttcc
    21781 attcataaaa attaaatcca caggtacagt gaaaatattt aattctccag caatagcttc
    21841 cacctgactt tgattaatca cagctaatgg cacattggga tatgattgca tcaagtcttc
    21901 aatttgtggt aaaacggcat gacacacgct acactgatcg cgcatgacat gtacaactgc
    21961 taacggatat tgatgaataa attcacggaa tgcttcgaca ctatttaatt gcttaaccat
    22021 ttcgaacacc ttcttacttt ttatatttta aaatgaagtc attattttgt tgctaaaatg
    22081 tcttgaaaca cttgtggcac agaggttatc ttcttatcac tcaatctgta aaaaccgcct
    22141 gtctgtgtac caatacaatg cacgccatgt gcggacacaa tttcaaactg aaacacccat
    22201 ttcatcttgc ttgctttaac taagtaaata cgacctactg gtttgtcatg tattgtgaca
    22261 ggtactttat actctgcttc cgttttcata agtataggtg aaatgccttg attgatcatt
    22321 gttgagtaat tcatgtgctg attaataaac gctgttctca aatcttcgaa ccatctaata
    22381 taaacgatat tactaacgac acccatcgca tcaatgtcgt aaccattcac ttcgatttct
    22441 ttctctgcaa ctaatagacc ttctgttgtc ataatgacaa atctccttat cttacaaata
    22501 gatttaaaat catgttaatt tatttttatt actttcatga taaaaataca agcaattgaa
    22561 ctttattacg gaaaatcaaa cttttcaatg agactattaa tttggctagt cgcgaagatg
    22621 catttttcta aaatgtgaca aaaacataag caatgttttc tatatctaac actaatattt
    22681 aacttattat catcaacaca cctgctatac atcattgcgc actaaaaagg cagtagcttc
    22741 attttaagct actgcctttt gtaacgtatt ctctttcaac tcgctacttc gcatacgcac
    22801 tacgaatgac taaaagttca ttcatttgat atgctgtttc atttttaatc attatttgac
    22861 tgtcataatt agcttacctg gatcagcatc ttgtacatct tcaatgacaa gttctttatt
    22921 ttcaagattt gtaataatca ttggtgtaac gatgcttggt gcgtgtgctt tcaagtatgc
    22981 taaattcact ttcattaatg gttgaccttg tgttactttt tcatcaacgt taatcagact
    23041 ttcgaatcct tcaccattca gtttcactgt atcgatacca atatgaataa gtacttcgac
    23101 gccactttca gattctaatc ctatcgcatg tttcgtaggg aagattgttt tcactgtacc
    23161 atcaaacggt gctacaattt cacctttttc agggataaag ccaacaccat cacccatcat
    23221 tttaccagcg aatacttgat caggtacttc tgataatgga atgatttgac cgatacctgg
    23281 tgcatagatg tctgttgttc caagtgcttc tacgtgaact ggttcatctg acatttcttc
    23341 agtcactgtc gtttcacttg gtttcgtaat ttcaccactc ataatcttgg ccatatcatg
    23401 tttaatttga tctgattttg gaccaaagat agcttgcata ttgtttccaa cttctaatac
    23461 acctgatgcg cctaaagctt taatacctgc tacatctact tttgatttat caaccacttc
    23521 tacgcgtaga cgtgtaatac atgcatctaa atgtttaatg ttttcttttc cacccattgc
    23581 atctaagaca tcaaatggta attttgcgac actagagtta cgaatttcag tttcttcatc
    23641 ttcacgacct ggtgttttca atttaaactt acgaattgca aagtcgaata agaagtaata
    23701 cacgatagca tatacaatac cgactggaat aactaataat gcgtgtgaac gatcccagtt
    23761 taataaacca tataaaatat aatctatgaa accacctgag aatgtcatac caattttaac
    23821 gcctaataaa tgcattacta agaatgatgt accagctaat aatacgtgaa ttccataaag
    23881 tactggtgct acaaataaga atgaaaattc taatggctca gtgataccag ttaaaaatgc
    23941 agttaatcct gctgataaca ttaaaccacc cacgactttt ttacgttctg gtcgtgcatt
    24001 tttataaata gcaaatgccg ccgctggtaa accaaacatc ataaatggat atttaccagt
    24061 agtaaatgca ccagcagtaa atggtacgcc atctttcaat tgtgccatcc aaatacgttg
    24121 gtcaccacga accaattcac ctgcgtgatt tgtataactt ccgaattcaa accagaacgg
    24181 tgaatagaaa atatgatgta aaccaaatgg aattaatgag cgttcaataa taccgaatat
    24241 aaacgttgtt aatgttaaat ttttatttaa taagaaattc gataaactat ttaatccatc
    24301 ttgaattggt ggccacgcaa agcttaaaag cacacctgtt gcgattgcta cgaccgatgt
    24361 cacaatcggt acaaatcgtt tacctgcaaa gaatcctaaa aatggtggta gtgtaatatt
    24421 ataaaatttg ttgtaacacc atgcggctaa agcacccatg ataataccac caaacacacc
    24481 cgtttgtaac gttggaatac ctaatactaa agcatgtgct ggttctttcg ctgcttgact
    24541 taattctttt gcccctttgg catatgagaa aatgtcatca attgtaatgt gcaacacttt
    24601 ccccattgtt gcattcataa ttaagtaacc tactagcgct gctaatgctg caacaccgtc
    24661 tcctcctgct aatccaagtg ctgtaccaac tgcaaataat aatggcaagt tatcaaatac
    24721 aacttgtcct gctgcttcca tgaccgacga aatcattaca atgatatcgt tttttaacca
    24781 tggtgcaatt tctactaatt gttcgttgtg cattgcgtta ccaaacgcta ataaaatacc
    24841 agctgctggt aaaatcgcaa caggtaacat taatgcttta ccgatacgtt gcaattgtcc
    24901 aaacaatttc ttaaacatat aaacatcccc tatctcaatt tccgcttcca tgtacaaaaa
    24961 aagcaggcat gaacaaacaa gactattctc atagtgcggt tgtttgctca tgcctgcatt
    25021 accagtaaca cgcaagtgat atctaattca tagctattat acatatttgt aaaagcgttt
    25081 tcaacaactt tttcacaatt ttatcatcaa ttcagtacga tcatttgtga taaataaaca
    25141 aaacggcaat tcccattttg ttgaaataga aattaccgtt tttatagttc aattcaattg
    25201 tgataacctt taaatcagtt ataagaaacg ttttgctgcc actgaaattg gtggaatttg
    25261 atctaaatct aaatgtttat cttcagtaat tgatctaaat gcccacttac cataaccaag
    25321 cgcttcttca tttacaattt tacctggtaa tggcgcagca ttaggatcaa catatacatc
    25381 tacaatcgtt ggtacatctt gtgctaatgc ctcttcgact atagcatcta cttcgctagc
    25441 actcttaatt gtataacctt taccacctgc tgcctcagca aattttgcat gatccatatc
    25501 agaaaaatca actgcatatt ctaattcacc agctgcttgt tgttcatatt taataaatgc
    25561 taactgtttg ttattaagta caaatacagt taaaggtaaa tcatattgta ctgctgtagc
    25621 gaagtcttgc attaccattt ggaatgcacc gtcaccagca attgcgatgg cttgtctatt
    25681 tggatatgca atttttgatg caattgcacc tggaagaccg caacccattg tacctaacca
    25741 acttgaaatg atgaacttgt tatttacacc aaggtttaag tatcgagttg accaaactgt
    25801 tgctgtacct acatctgctg aaatcactgc atcatcttta ataaatttat tgattgatgc
    25861 cattaatcgt tctggacgta atggtttact attattattt ttatcttgtt ccatccattt
    25921 atcccaaacc gctttacgtt ctaacgtttt gtttaagaat ggtctttcag caacatgttt
    25981 aatattttca gttaactgat gcaacgcaat tttactatct ccaacaattc ctacattaat
    26041 attgaaacga tgtccgatat ttttaggatt tgtgtcaatt tgaatggctt taatattttt
    26101 cttaggtaag taatccacat atggatagtt tgtaccaacc ataattaata aatccgcttc
    26161 ctgcattgtt tgataagatg ttttggtacc gattttacct aagttaccga tactatatgg
    26221 atgatcatcc ggcaagattg ttttagctgg taatgaatga atgacaggaa ttttagccat
    26281 ttcaataaat tcacgtagct catctttcgc atgtttcgca cctacaccaa ttaacatgac
    26341 aggcttttta cttttattaa ttagtttaac cgcttttttg atgtctttat attttggtga
    26401 tactactgtt ggtcttgatg tatctactgg tttattcgtt gtatctttaa ttttttcagt
    26461 taataagtcg ttaggacaaa taacaacagc tacacctttt tgttcatatg ccgtacgaat
    26521 tgcttcgtta acgatttcaa acacattgtc acctttttca atttggtgat tataaacggc
    26581 tacatcttca cataattttt gtaaatttgt ttcttggaat gctttcgttc caagtgctgt
    26641 actattcgtt tgtccagata atattaattg cggtacatta tccattttgg catcatacat
    26701 accatttaat aaatgaatta aaccagggcc accgatactt aatgccacac cgattttacc
    26761 agttaatttt gtgtaaccag cagccgctaa gcttgctact tcttcatgac gtacatgata
    26821 aaatttaaat tgatctctca ctgtacgtaa actatcgact actgcgtcga ttgagtctcc
    26881 tggaatacca tacaagtgat ctatatccca tgcttgtaat gctttaacta atgcttcatt
    26941 tgcttttatt tttgccatta ttactaatag cctccctttc tgtctatcat tttaaatatc
    27001 taaattaata gaataataaa attagaacag gtattaacac tgaactaata actgcagtta
    27061 aaatcatccc aattgaacta aatgcaccgg attcaatatc catttctagt gctttagctg
    27121 tgccaaatgc atgtgacgca ttcccatacg ttaatccttt ggcgatagaa gattcaaatc
    27181 ttccaaatct taataacatc gaacctaaaa tactaccgat taaacccgtt gtgataataa
    27241 aaagtacggt catcgtatct gtaccaccta gttcatgtga cacttcgata cctactgcgg
    27301 ctgttataga tcggggcaat aacgttacaa tgacgtcttt agaatagcca aatgctttaa
    27361 gtgttaagaa cactaacatg aaattcagca tgacgccagt taatacactt gcaaaaatga
    27421 tagagacatt gtctttaatt ttctctctat ttttataaag tgggtacgct aaacatacga
    27481 ccgttgcgtt taaaatatgg ttgatccaac tgccaccttt catatacccg ttataactaa
    27541 ttccaaagat aagtaagaca aaaataattc ctaaagatgc aattaatgct ggattcaaaa
    27601 atgggttcgg atatttttgt tgtaaccttt tagcgaaata atataaaacg acagtcaaca
    27661 aaatcattaa taaggcttgc acgtaatcat tcataagcgt ctacaccttt acgatgttta
    27721 tgtttaacag acattttttc agcaatataa cctgaagata atgcaacgat acatgttcct
    27781 atgataatga ctgcgaaaaa gagtatataa tttagcgtaa tttcggaagc aacatccata
    27841 atacctacaa ctgacggtat gaagaaaaag accatcgtct ttaataaaaa gtttgcaccg
    27901 tcttctaccc aagttagcgg tacaatctta aattgtaata gtaaataaaa taaaaataga
    27961 ccaacaatac tgccggctaa gggaagatga aaaatctttt gaatttctgt gccaatataa
    28021 gtaatcacaa tgatgattcc tagttgtagt agtagtttga ttattaattg gactttgtgc
    28081 atggcgccat ccctttctaa atatgtctaa attgttacaa taactattat aaagatggcg
    28141 tacacaatat aaaaatactc tttttttata gtttgtatta ctattttcta tggaaacgct
    28201 ctctaaattt cacttcattt tcacaaaaaa acactcatta tttcaatgac ccacgtaatg
    28261 atgcgcctta aataatgagt gctttattaa tatgttagcc taaacggtct ttcaaaaatt
    28321 ctatccattt cgttgtggca tgacttaatt gtttatcctt cttccaaaca acacctaatt
    28381 cccaatgtac atgagcatct tcaatgcgta gcagcttcac atctccattt agttgctctg
    28441 aaattgatgt tggtaaaata ctaataccta attcattcgt aactaaatct tctataacat
    28501 gccattgtga aatttgcgct acagtattcg gaacaaagcc aacgttttta gcattttcaa
    28561 taattttatc attcaagtaa aagtctttat taaataaaat gaagtcttca cctgctaaat
    28621 cttcgagttt aacagtttca tattttgcga gtcgatgctc tctgctcacg ataagtcgca
    28681 aatcttcctt atctagggta gtataatcga aaatatgatg atcgactggc aaagtggtca
    28741 cgcctatatc tacttcatca ttgataattt gctgttcaat tgttttaccg ccattttcta
    28801 tcaaattata tgtaacattt ggatattgtt gatggaatgc accaagaata ttgataagaa
    28861 tcttcatatt catgactgcc gacatgccca tgtttatatg tcctgtttcc agtccattca
    28921 agcgttccat ttcagatggt aaataatcat acagtgcaac aatttcttta cttttctcat
    28981 aaaaaatttg acctgcatcg gtaagaatta aatgtctttt acttctatca aataagggcg
    29041 tgcccatttc attttcaata tctttaattg ctttactaat tgtaggttgt gcaatatata
    29101 atgatttgga agcattagtc atgcctcctt gcttaacgac ttcaataaaa tatttcatat
    29161 gtttgatatc cactaacatc atcctaacta tttcaacacg tttatttcat tatacacaac
    29221 ttcttaaaca ttcaataaat ttagatttat aaaattgctt tataaaaagt tttatgtaaa
    29281 atacacattc ttttatggta ttatattaat ggctcaagta tattaatcga ggtgaggtat
    29341 tgagctttta cgaatttatg caaagttttc ttggtgatga cacaccatta ggtgaattgg
    29401 ttgattggat taatcaagat agcaatttcc ctagagaagt gaagagccat agtgaaatat
    29461 tgtcatattt tcgaacaaat ccatgtcctg aaagcatctc agtacctgta attaaacggg
    29521 cactatcagt tttcaaccaa ttcacaaatg tacaatgata catgaataca tggagtcaca
    29581 tcatctcatg ttagcgctct gattgcaggg cgctattttt aatgttattg ctacatttag
    29641 tatattgtga ttgctatttt ctttcagtta tagaaccacg taccttttct tttaaatcat
    29701 tttatatttt cccactaata tccgctgtta atcaatcctg acatccttgt atcactatga
    29761 caattaattg ttaaatacat gaatttctac attttatgaa aaaatccatt tttattacaa
    29821 ttcaacactt tatatgacaa cttcattaca gttactttta ttgttgattg cttacattgt
    29881 tttctaaaaa aaatttgtta tcataattaa cgttgaataa agaaaaaaat taacttggga
    29941 gataaaaatg gaatataaaa agatactaat tcgtttatta attgcttttg cagtactttt
    30001 ctcagcagat ttcacttatc aatccgtcga acaaacgcat caatcgcatg ccgcagttaa
    30061 ttattatagt aaaaaccaat gtacatggtg ggcatttaaa cgtcgcgcac aagtcggtaa
    30121 acctgtttct aatagatggg gcaatgctaa aaattggtat tacaatgcac gtaaatcaaa
    30181 atatgcgact ggtcgtacac caagaaaatt tgctgtcatg caatcaactg caggatatta
    30241 tggacatgtc gcagttgttg aacaagtata taaaaacggt agtattaaag tttcagaata
    30301 caacttttat cgcccattaa aatacaatac acgtgtacta agcaaaaagg cagcacgtaa
    30361 ctttaacta
//
LOCUS       DAXWFK010000012        29824 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.012, whole genome shotgun sequence.
ACCESSION   DAXWFK010000012 DAXWFK010000000
VERSION     DAXWFK010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 29824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29824
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.012"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(477..1754)
                     /locus_tag="ACGEXS_000446"
     CDS             complement(477..1754)
                     /locus_tag="ACGEXS_000446"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFF8011494.1"
                     /translation="MQNLDKNFRHLSRKEKLQQLVDKQWLSEEQFDILLNHPLIDEEV
                     ANSLIENVIAQGALPVGLLPNIIVDDKAYVVPMMVEEPSVVAAASYGAKLVNQTGGFK
                     TVSSERIMIGQIVFDGVDDTEKLSADIKALEKQIHKIADEAYPSIKARGGGYQRIAID
                     TFPEQQLLSLKVFVDTKDAMGANMLNTILEAITAFLKNEFPQSDILMSILSNHATASV
                     VKVQGEIDVKDLARGERTGEEVAKRMERASVLAQVDIHRAATHNKGVMNGIHAVVLAT
                     GNDTRGAEASAHAYASRDGQYRGIATWRYDQDRQRLIGTIEVPMTLAIVGGGTKVLPI
                     AKASLELLNVESAQELGHVVAAVGLAQNFAACRALVSEGIQQGHMSLQYKSLAIVVGA
                     KGDEIAKVAEALKKEARANTQAAEHILQEIRQQ"
     gene            2008..3174
                     /locus_tag="ACGEXS_000447"
     CDS             2008..3174
                     /locus_tag="ACGEXS_000447"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFF8011495.1"
                     /translation="MTIGIDKINFYVPKYYVDMAKLAEARQVDPNKFLIGIGQTEMAV
                     SPVNQDIVSMGANAAKDIITDEDKKKIGMVIVATESAVDAAKAAAVQIHNLLGIQPFA
                     RCFEMKEACYAATPAIQLAKDYLATRPNEKVLVIATDTARYGLNSGGEPTQGAGAVAM
                     VIAHNPSILALNEDAVAYTEDVYDFWRPTGHKYPLVDGALSKDAYIRSFQQSWNEYAK
                     RQGKSLADFASLCFHVPFTKMGKKALESIIDNADETTQERLRSGYEDAVDYNRYVGNI
                     YTGSLYLSLISLLENRDLQAGETIGLFSYGSGSVGEFYSATLVEGYKDHLDQAAHKAL
                     LNNRTEVSVDAYETFFKRFDDVEFDEEQDAVHEDRHIFYLSNIENNVREYHRPE"
     gene            complement(3344..3865)
                     /locus_tag="ACGEXS_000448"
     CDS             complement(3344..3865)
                     /locus_tag="ACGEXS_000448"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFF8011496.1"
                     /translation="MEYKSYYDSPVGRLELLSDGVSLTAVLFENQQGDGTREENTSLA
                     IFREATQWLDAYFKGDNPEITIPLKPTGSHFQQCVWNELRQIPYGTLTTYGAIAKKVG
                     KLLNKPKMSAQAVGGAVGSNPLSIIVPCHRVVGKTGSLTGFGGTINNKIKLLELENID
                     MSKLYVPKHSTKP"
     gene            4274..6379
                     /gene="clpL"
                     /locus_tag="ACGEXS_000449"
     CDS             4274..6379
                     /gene="clpL"
                     /locus_tag="ACGEXS_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpL"
                     /protein_id="HFF8011497.1"
                     /translation="MNNGFFNSDFDSIFRRMMQDMQGSNQVGNKKYYINGKEVSPEEL
                     AQLTQQGGNHSAEQSAQAFQQAAQRQQGQQGGNGNYLEQIGRNLTQEARDGLLDPVIG
                     RDKEIQETAEVLSRRTKNNPILVGEAGVGKTAIVEGLAQAIVEGNVPAAIKDKEIISV
                     DISSLEAGTQYRGAFEENIQKLIEGVKSSQNAVLFFDEIHQIIGSGATGSDSGSKGLS
                     DILKPALSRGEISIIGATTQDEYRNNILKDAALTRRFNEVLVNEPSAKDTVEILKGIR
                     EKFEEHHQVKLPDDVLKACVDLSIQYIPQRLLPDKAIDVLDITAAHLSAQSPAVDKVE
                     TEKRISELENDKRKAVSAEEYKKADDIQNEIKSLQDKLENSNGEHTAVATVHDISDTI
                     QRLTGIPVSQMDDNDIERLKNISNRLRSKIIGQDQAVEMVSRAIRRNRAGFDDGNRPI
                     GSFLFVGPTGVGKTELAKQLAIDLFGNKDALIRLDMSEYSDTTAVSKMIGTTAGYVGY
                     DDNSNTLTEKVRRNPYSVILFDEIEKANPQILTLLLQVMDDGNLTDGQGNVINFKNTI
                     IICTSNAGFGNGNDAEEKDIMHEMKKFFRPEFLNRFNGIVEFLHLDKDALQDIVNLLL
                     DDVQVTLDKKGITMDVSQDAKDWLIEEGYDEELGARPLRRIVEQQVRDKITDYYLDHT
                     DVKHVDIDVEDNELVVKGK"
     gene            complement(6448..6618)
                     /locus_tag="ACGEXS_000450"
     CDS             complement(6448..6618)
                     /locus_tag="ACGEXS_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFF8011498.1"
                     /translation="MSVIINILIFLAIFGYALYTLVKFFKRSKQGKCGTCDINRDCCG
                     TEQHTANHFPGK"
     gene            complement(6631..8625)
                     /gene="feoB"
                     /locus_tag="ACGEXS_000451"
     CDS             complement(6631..8625)
                     /gene="feoB"
                     /locus_tag="ACGEXS_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFF8011499.1"
                     /translation="MENYCILGNPNVGKTSLFNALTGSYEYIGNWSGVTVEKKVGKLK
                     ENVGQLIDLPGTYDLSPISKDETVVTDYLLNDSFSGIINIVDASQLKRNMQLTVQLLE
                     LNQPIYIGLNMIDVATKRGIKIDYHKLMKKLKTPIFPVVARTGKGTKYLLGEIKHLGE
                     GYQPHFKINYGEKIEETIKNMCQIIMTETSHDKYQARFIAIQFLLNNMQIANELNSEV
                     VNKLSSLRDQVAEQVGAVSVRREMERIRNHYIETLLQDVVTYPDEDKQYFSSRIDKIL
                     THKYIGMPIFLAIMWLIFQTTFTWIGTPLSDQLDAFIGGTFTDSVKTIMNYLGVIPFL
                     QDLITDGIIAGVGSVLVFVPQIVVLFFFISLLEDSGYMARIAVLMDRIMESFGLSGKS
                     FIPMIIGFGCNVPSIMAARSIENEKERLTTILIAPFMSCSARLPVYALFVGIFFKENQ
                     SLVVLSLYVLGIIMAFLVSTVLTKTILKNDNAIFIVELPTYRVPSIKTLWRSTWEKAK
                     GFVRKAGTFIFGGSVVIWLLSYVGPHGINVNINQSFLHMVGSFFGMLVQPLGFGTWQA
                     GATLVPGFLAKEVIVSSMAIIYSSGDAGLVNVIQNQFTPLSAYAFMIFILLYIPCVST
                     VAAIRKETYSWKWTALAVAYPLVTAYVLTFIFYQVGHLFV"
     gene            complement(8637..8864)
                     /locus_tag="ACGEXS_000452"
     CDS             complement(8637..8864)
                     /locus_tag="ACGEXS_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFF8011500.1"
                     /translation="MLNIKNGEMNKAYKIKRMDIANENMLYRLSAFGLTDDAIITIKQ
                     KCLFKGPCIIEVNGQQLSIRHCDACSIALEE"
     gene            complement(9080..11569)
                     /gene="farE"
                     /locus_tag="ACGEXS_000453"
     CDS             complement(9080..11569)
                     /gene="farE"
                     /locus_tag="ACGEXS_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid efflux MMPL transporter FarE"
                     /protein_id="HFF8011501.1"
                     /translation="MAKFLYKMGTFIAKHKWSAVIAWIVIVAAILIPLATNAPKFDND
                     IKMTGLESLDTNKKIEKHFNQDSEKAQIRVVFKTTKDDGIVQPNITKDIKKTLDDIKK
                     DDKHIDKISDPYENKQISKDKTTAFADITYDVSQTSLKDGSRDNVKSHLKDLRDNHNV
                     QTELTGTGMTSTEVGGNSELVGIIVAFVVLLITFGSVIAAGLPIISALIGLASGVGII
                     SLLTYAFDIPNVTLTLAVMIGLAVGIDYALFILFRYRQVMKTETDYIKGIGLAIGTAG
                     SAVVFAGVTVVIAVCGLSLVGIDFLAVMGFASAISVVFAVLSALTLLPALISIFHKRI
                     KVNKLQSKFKKDIDTPWSKFITGNALAAVLLGLIILVAAAIPVSHMRLGIPDDGVKPA
                     DSTQKKAYDIISDKFGEGFNGQIPMLINVKDKKDDPQGLQQDLQSVYKDIKDKKNVDI
                     VTPPQMSKDNDYALMVVIPKQGPNAESTNDLVHDLRDYHKDAQDKYGFKTEISGQSVI
                     NIDMSKKLNEAIPLFASVIVVLAFFLLMIVFRSILIPLKAVLGFVLSLMATLGFTTLV
                     MQDGFMKGLFGIETTGPMLAFLPVITIGILFGLAMDYEVFLMSRIHEEYSKTGDNDYS
                     IKVGLKESGPVIVAAALIMFSVFFAFVFQEDVMIKSMGMALAFGVLFDAFVVRMMLIP
                     ALTKLFGKGSWYLPAWLNRIIPRVDIEGHALEKYKTVESQESEAKDSKETYDTTFKVY
                     PQGATNVSKHQDAHGQDDAHSIVLDDKTMALYQEVKQQSASSLFLYDALIDYQNKHQL
                     NSKQQATNIEQLNKNIEKLNQLLEKNLRNKS"
     gene            11714..12262
                     /gene="farR"
                     /locus_tag="ACGEXS_000454"
     CDS             11714..12262
                     /gene="farR"
                     /locus_tag="ACGEXS_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid efflux pump transcriptional regulator
                     FarR"
                     /protein_id="HFF8011502.1"
                     /translation="MKETDLRVIKTKKALSSSLLQLLEQQLFQTITVNQICDNALVHR
                     TTFYKHFYDKYDLLEYLFNQLTKDYFARDISDRLNHPFQTMSDTINNKEDLREIAEFQ
                     EEDAEFNKVLKNVCIKIMHNDIKNNRDRIDIDSDIPDNLIFYIYDSLIEGFIHWIKDE
                     KIDWPGEDIDNIFHRLINIKIK"
     gene            complement(12339..12506)
                     /locus_tag="ACGEXS_000455"
                     /pseudo
     CDS             complement(12339..12506)
                     /locus_tag="ACGEXS_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501324.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12618..14162)
                     /gene="pruA"
                     /locus_tag="ACGEXS_000456"
     CDS             complement(12618..14162)
                     /gene="pruA"
                     /locus_tag="ACGEXS_000456"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HFF8011503.1"
                     /translation="MVVEFKNEPGYDFSVQENVDMFKKALKDVEKELGQDIPLVINGE
                     KIFKDDKIKSINPADTSQVIANASKATKQDVEDAFKAANEAYKSWKTWSANDRAELML
                     RVSAIIRRRKAEIAAIMVYEAGKPWDEAVGDAAEGIDFIEYYARSMMDLAQGKPVLDR
                     EGEHNKYFYKSIGTGVTIPPWNFPFAIMAGTTLAPVVAGNTVLLKPAEDTPYIAYKLM
                     EILEEAGLPKGVVNFVPGDPKEIGDYLVDHKDTHFVTFTGSRATGTRIYERSAVVQEG
                     QNFLKRVIAEMGGKDAIVVDENIDTDMAAEAIVTSAFGFSGQKCSACSRAIVHKDVYD
                     EVLEKSIKLTKELTLGNTVDNTYMGPVINKKQFDKIKNYIEIGKEEGKLEQGGGTDDS
                     KGYFVEPTIISGLKSKDRIMQEEIFGPVVGFVKVNDFDEAIEVANDTDYGLTGAVITN
                     NREHWIKAVNEFDVGNLYLNRGCTSAVVGYHPFGGFKMSGTDAKTGSPDYLLHFLEQK
                     VVSEMF"
     gene            complement(14352..14951)
                     /locus_tag="ACGEXS_000457"
     CDS             complement(14352..14951)
                     /locus_tag="ACGEXS_000457"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HFF8011504.1"
                     /translation="MTEKEKMLAEKWYDANFDQYLINERARAKDICFELNHTRPSATN
                     KRKELIDQLFQTTTDNVSISIPFDTDYGWNVKLGKNVYVNTNCYFMDGGQITIGDNVF
                     IGPNCGFYTATHPLNFHHRNEGFEKAGPIHIGSNTWFGGHVAVLPGVTIGEGSVIGAG
                     SVVTKDIPPHSLAVGNPCKVVRKIDNDLPSETLNDETIK"
     gene            15190..15381
                     /gene="cwrA"
                     /locus_tag="ACGEXS_000458"
     CDS             15190..15381
                     /gene="cwrA"
                     /locus_tag="ACGEXS_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall inhibition responsive protein CwrA"
                     /protein_id="HFF8011505.1"
                     /translation="MRILITGTVAILIILGLVKTIQDYEMTNDTSRQLSDNKDDDKVI
                     HLNNFKNLHAKEFNPSDFF"
     gene            15621..18029
                     /locus_tag="ACGEXS_000459"
     CDS             15621..18029
                     /locus_tag="ACGEXS_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFF8011506.1"
                     /translation="MANTKKTTLDITGMTCAACSNRIEKKLNKLDDVNAQVNLTTEKA
                     TVEYNPDQHDVQEFINTIQHLGYGVAVETVELDITGMTCAACSSRIEKVLNKMDGVQN
                     ATVNLTTEQAKVDYYPEETDADKLVTRIQKLGYDASIKDNNKDQTSRKAEALQHKLIK
                     LIISAVLSLPLLMLMFVHLFNMHIPALFTNPWFQFILATPVQFIIGWQFYVGAYKNLR
                     NGGANMDVLVAVGTSAAYFYSIYEMVRWLNGSTTQPHLYFETSAVLITLILFGKYLEA
                     RAKSQTTNALGELLSLQAKEARILKDGNEVMIPLNEVHVGDTLIVKPGEKIPVDGKII
                     KGMTAIDESMLTGESIPVEKNVDDTVIGSTMNKNGTITMTATKVGGDTALANIIKVVE
                     EAQSSKAPIQRLADIISGYFVPIVVGIALLTFIVWITLVTPGTFEPALVASISVLVIA
                     CPCALGLATPTSIMVGTGRAAENGILFKGGEFVERTHQIDTIVLDKTGTITNGRPVVT
                     DYHGDNQTLQLLATAEKDSEHPLAEAIVNYAKEKQLILTETTTFKAVPGHGIEATIDH
                     HHILVGNRKLMADNDISLPKHISDDLTHYERDGKTAMLIAVNYSLTGIIAVADTVKDH
                     AKDAIKQLHDMGIEVAMLTGDNKNTAQAIAKQVGIDTVIADILPEEKAAQIAKLQQQG
                     KKVAMVGDGVNDAPALVKADIGIAIGTGTEVAIEAADITILGGDLMLIPKAIYASKAT
                     IRNIRQNLFWAFGYNIAGIPIAALGLLAPWVAGAAMALSSVSVVTNALRLKKMRLEPR
                     RKDA"
     gene            18182..18388
                     /gene="copZ"
                     /locus_tag="ACGEXS_000460"
     CDS             18182..18388
                     /gene="copZ"
                     /locus_tag="ACGEXS_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFF8011507.1"
                     /translation="MSQEILNVEGMSCGHCKSAVESALNNIDGVTSADVNLENGQVSV
                     QYDDSKVAVSQMKDAIEDQGYDVV"
     gene            complement(18478..19476)
                     /locus_tag="ACGEXS_000461"
     CDS             complement(18478..19476)
                     /locus_tag="ACGEXS_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFF8011508.1"
                     /translation="MTKIKIMSVRDEDMPYIKAWAEKHHVEVDITKEALTDDNVEGVA
                     GYDGLSLSQQIPLSEHVYKRLNELGIKQIAQRSAGFDTYDLELANKYNFIVSNVPSYS
                     PNSIAEFAVNQAINVVRHFNQIQTKVREHDFRWEPTILSKSIKDLKVAVIGTGRIGRV
                     VADIFANGYQSDVVAYDPFPNAKIATYVDYKDTIEEAVEGADIVTLHVPATKYNHYLF
                     NAELFKHFKKGAVFVNCARGCLVDTKALLDALDNGVIKGAALDTYEFERKLFPSDQRG
                     KTLNDPLLESLIDREDVILTPHIAFYTEAAVKNLIVDALDATLDVLQTGDTRLRVN"
     gene            complement(19499..20653)
                     /locus_tag="ACGEXS_000462"
     CDS             complement(19499..20653)
                     /locus_tag="ACGEXS_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFF8011509.1"
                     /translation="MISNKLANIPDSYFGKTMGRKIEHGPLPLINMAVGIPDGPTPQG
                     IIDHFQKALTIPENQKYGAFHGKEAFKQAIVDFYQRQYNVTLDKEDEVCILYGTKNGL
                     VAVPTCVINPGDYVLLPDPGYTDYLAGVLLADGKPVPLNLEPPHYLPDWSKVDSQIID
                     KTKLIYLTYPNNPTGSTATKEVFDEAIAKFKGTDTKIVHDFAYGAFGFDAKNPSILAS
                     ENGKDVAIEIYSLSKGYNMSGFRVGFAVGNKDMIQALKKYQTHTNAGMFGALQDAAIY
                     ALNHYDDFLEEQSNVFKTRRDRFEAMLAKADLPFVHAKGGIYVWLETPPGYDSEQFEQ
                     FLVQEKSILVAPGKPFGENGNRYVRISLALDDQKLDEAAIRLTELAYLYE"
     gene            complement(21080..22588)
                     /locus_tag="ACGEXS_000463"
     CDS             complement(21080..22588)
                     /locus_tag="ACGEXS_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HFF8011510.1"
                     /translation="MKIAVIGAGVTGLAAAARIASQGHEVTIFEKNNNVGGRMNQLKK
                     DGFTFDMGPTIVMMPDVYKDVFTACGKNYEDYIELRQLRYIYDVYFDHDDRITVPTDL
                     AELQQMLESIEPGSTHGFMSFLTDVYKKYEIARRYFLERTYRKPSDFYNMTSLVQGAK
                     LKTLNHADQLIEHYIDNEKIQKLLAFQTLYIGIDPKRGPSLYSIIPMIEMMFGVHFIK
                     GGMYGMAQGLAQLNKDLGVNIELNAEIEQIIIDPKFKRADAIKVNGDIRKFDKILCTA
                     DFPSVAESLMPDFAPIKKYPPHKIADLDYSCSAFLMYIGIDIDVTDQVRLHNVIFSDD
                     FRGNIEEIFEGRLSYDPSIYVYVPAVADKSLAPEGKTGIYVLMPTPELKTGSGIDWSD
                     EALTQQIKEIIYRKLATIEVFEDIKSHIVSETIFTPNDFEQTYHAKFGSAFGLMPTLA
                     QSNYYRPQNVSRDYKDLYFAGASTHPGAGVPIVLTSAKITVDEMIKDIERGV"
     gene            complement(22600..23463)
                     /gene="crtM"
                     /locus_tag="ACGEXS_000464"
     CDS             complement(22600..23463)
                     /gene="crtM"
                     /locus_tag="ACGEXS_000464"
                     /EC_number="2.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,4'-diapophytoene synthase CrtM"
                     /protein_id="HFF8011511.1"
                     /translation="MTMMDMNFKYCHKIMKKHSKSFSYAFDLLPEDQRKAVWAIYAVC
                     RKIDDSIDVYGDIQFLNQIKEDIQSIEKYPYEHHHFQSDRRIMMALQHVAQHKNIAFQ
                     SFYNLIDTVYKDQHFTMFETDAELFGYCYGVAGTVGEVLTPILSDHETHQTYDVARRL
                     GESLQLINILRDVGEDFDNERIYFSKQRLKQYEVDIAEVYQNGVNNHYIDLWEYYAAI
                     AEKDFQDVMDQIKVFSIEAQPIIELAARIYIEILDEVRQANYTLHERVFVDKRKKAKL
                     FHEINSKYHRI"
     gene            complement(23500..24627)
                     /gene="crtQ"
                     /locus_tag="ACGEXS_000465"
     CDS             complement(23500..24627)
                     /gene="crtQ"
                     /locus_tag="ACGEXS_000465"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,4'-diaponeurosporenoate glycosyltransferase"
                     /protein_id="HFF8011512.1"
                     /translation="MKWLSRILTVIVTMSMACGALIFNRRHQLKAKTLNFNHKALTII
                     IPARNEEKRIGHLLHSIIQQQVPVDVIVMNDGSTDETARVARSYGATVVDVVDDTDGK
                     WYGKSHACYQGVTHACTNRIAFVDADVTFLRKDAVETLINQYQLQGEKGLLSVQPYHI
                     TKRFYEGFSAIFNLMTVVGMNVFSTLDDGRTNQHAFGPVTLTNKEDYYATGGHKSANR
                     HIIEGFALGSAYTSQSLPVTVYEGFPFVAFRMYQEGFQSLQEGWTKHLSTGAGGTKPK
                     IMTAIVLWLFGSIASILGLCLSLKYRQMSVRKMVALYLSYTTQFIYLDRRVGQFSNLL
                     MVCHPLLFMFFTKIFIQSWKQTHRYGVVEWKGRQYSISKEQ"
     gene            complement(24633..26126)
                     /locus_tag="ACGEXS_000466"
     CDS             complement(24633..26126)
                     /locus_tag="ACGEXS_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HFF8011513.1"
                     /translation="MTKHIIVIGGGLGGISAAIRMAQSGYSVSLYEQNNHIGGKVNRH
                     ESDGFGFDLGPSILTMPYIFEKLFEYSKKQMSDYVTIKRLPHQWRSFFPDGTTIDLYE
                     GIKETGQHNAILSKQDIEELQNYLNYTRRIDRITEKGYFNYGLDTLSQIIKFHGPLNA
                     LINYDYVHTMQQAIDKRISNPYLRQMLGYFIKYVGSSSYDAPAVLSMLFHMQQEQGLW
                     YVEGGIHHLANALEKLAREEGVTIHTGARVDNIKTYQRRVTGVRLDTGEFVKADYIIS
                     NMEVIPTYKYLIHLDTQRLNKLEREFEPASSGYVMHLGVACQYPQLAHHNFFFTENAY
                     LNYQQVFHEKVLPDDPTIYLVNTNKTDHTQAPVGYENIKVLPHIPYIQDQPFTTEDYA
                     KFRDKILDKLEKMGLTDLRKHIIYEDVWTPEDIEKNYRSNRGAIYGVVADKKKNKGFK
                     FPKESQYFENLYFVGGSVNPGGGMPMVTLSGQQVADKINAREAKNRK"
     gene            complement(26119..26607)
                     /gene="crtO"
                     /locus_tag="ACGEXS_000467"
     CDS             complement(26119..26607)
                     /gene="crtO"
                     /locus_tag="ACGEXS_000467"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl-4,4'-diaponeurosporenoate
                     acyltransferase"
                     /protein_id="HFF8011514.1"
                     /translation="MKKYIKTAFFCSMYWLIVQLNIANLGTRIPDKYFRQKYIIFKSF
                     NFEKHGKFWNKWFYVRKWKHKILDGHQLNQNIYDQRHLMTINTDEIEKMIIETKRAEL
                     IHWISILPVIIFNKGPRLVKYINIFYAMIANVPIIIVQRYNRPRLTQLLRILKRRGER
                     HD"
     gene            complement(26782..27549)
                     /locus_tag="ACGEXS_000468"
     CDS             complement(26782..27549)
                     /locus_tag="ACGEXS_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HFF8011515.1"
                     /translation="MKKIVTATIATAGLATIAFAGHDAQAAEQNNNGYNSNDAQSYSY
                     TYTIDAQGNYHYTWTGNWNPSQLTQNNTYYYNNYNTYSYNNASYNNYYNHSYQYNNYT
                     NNSQTATNNYYTGGSGASYSTTSNNVHVTTTAAPSSNGRSISNGYASGSNLYTSGQCT
                     YYVFDRVGGKIGSTWGNASNWANAAASSGYTVNNTPKVGAIMQTTQGYYGHVAYVEGV
                     NSNGSVRVSEMNYGHGAGVVTSRTISANQAGSYNFIH"
     gene            complement(27910..29721)
                     /locus_tag="ACGEXS_000469"
     CDS             complement(27910..29721)
                     /locus_tag="ACGEXS_000469"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFF8011516.1"
                     /translation="MDTKDFKRLEKMYSPRYLPGLDGLRAFAVIGIIIYHLNAQWLSG
                     GFLGVDTFFVISGYLITSLLISEYYRTQKIDLLEFWKRRLKRLIPAVLFLICVVLTFT
                     LIFKPELIIQMKRDAIAAIFYVSNWWYISQNVDYFNQFAIEPLKHLWSLAIEEQFYLL
                     FPLVITFLLHRFKPRNIIQTLFIVSLISLGLMIVIHFITGDNSRVYFGTDTRLQTLLL
                     GCILAFIWPPFALKKDISKKIVVSLDIIGISGFAVLMTLFFIVGDQDQWIYNGGFYII
                     SFATLFIIAIAVHPSSLFAKFLSMKPLLIIGKRSYSLYLWHYPIIVFVNSYYVQGQIP
                     VYVYIIEILLTALMAEISYRFIETPIRKKGFKAFAFLPKKKGQFARTVLVILLLVPSI
                     VVLSGQFDALGKQHEAEKKEKKTEFKTTKKKVVKKDKQEDKQTANSKEDIKKSSPLLI
                     GDSVMVDIGNVFTKKIPNAQIDGKVGRQLVDATPIVKSQYKDYAKKGQKVVVELGTNG
                     AFTKDQLNELLDSFGKADIYLVSIRVPRDYEGRINKLIYEAAEKRSNVHLVDWYKASA
                     GHPEYFAYDGIHLEYAGSKALTDLIVKTMETHATNKK"
BASE COUNT         9601 a         5672 c         4615 g         9936 t
ORIGIN      
        1 atcttattat tacttctttt taacgctagt ggaataatta ataataattg tatttaaatg
       61 caatgctgtt gttctttcat acttacaagc aagtcataag aaatgagaaa ttaaattctt
      121 atatgatgaa taaatgatga ctgcagaaga aattgctaaa aaaggagtgg agtgaggcat
      181 tctactgttc atattgttaa caaatatcaa aatgatagga acttgaagaa tctaagactg
      241 ttaaaatcga gacaagaaga aactcgttcc aaatttgaat cctataatga attcgtctca
      301 tgaagaagtt atttactttt cattagactg atttactaat caaaaaactt ctatcacgaa
      361 cgcttcaatt tcctgtatgc gtgtgataga agtttttatt tttgttcata ttaatttatc
      421 taagcgctac gatggactga cttaaacttt tctttaaaat cgctattcgc catttactat
      481 tgttgtctaa tttcttgtaa aatatgttcc gctgcttgtg tatttgcacg ggcttctttt
      541 ttcaaagctt cagctacttt agcaatttca tcaccttttg cccctacaac gatagctaat
      601 gatttatatt gtaaactcat atgaccttgt tgaatacctt ctgacacaag cgcgcgacat
      661 gctgcaaagt tttgcgctaa accaacggca gcaactacat gacctaattc ttgtgctgac
      721 tctacattta gtagctctaa tgaagcttta gcaattggta atacttttgt accaccgcca
      781 acgattgcca atgtcatagg cacttcaatt gtaccaatca atcgttgacg atcttgatcg
      841 taacgccatg tagcaatacc acgatactgt ccgtcacgac tcgcgtatgc atgcgcactt
      901 gcttctgcac cacgcgtatc atttcctgtt gctaaaacaa cagcatgtat gccattcata
      961 acacctttat tatgtgttgc tgcacgatga atatctactt gggccaatac agaagcacgt
     1021 tccattcgtt tggcaacctc ttctccagtt ctctcgcccc ttgctaaatc tttaacatca
     1081 atttcgcctt gaactttaac aacggacgct gttgcatgat tggataaaat actcattaaa
     1141 atgtcgcttt gcggaaattc attttttaaa aatgcagtta tggcctctaa aatcgtatta
     1201 agcatattag cgcccatagc atctttcgta tcaacaaata cttttaaaga tagtaactgt
     1261 tgctcaggaa atgtatcaat cgctatacgt tggtaaccac caccacgcgc tttaatagaa
     1321 ggatatgcct catccgcaat tttatgaatt tgcttttcta aagctttaat gtctgctgat
     1381 aatttttcag tatcgtcaac gccatcaaag acgatttgac ctatcataat acgttcagaa
     1441 gataccgttt taaatccgcc agtctgattc actagctttg caccataact agctgcagcg
     1501 acaactgaag gctcttccac catcataggt acaacatatg ccttatcgtc cacaatgata
     1561 ttcggtaata atccaacggg taatgcacct tgcgcgatga cattttcaat taaactattg
     1621 gctacttctt catcgattaa tggatgattc agtaaaatgt cgaattgttc ttctgataac
     1681 cattgcttat caaccaattg ttgtaacttt tctttacgag ataaatgtcg aaaattctta
     1741 tctaaatttt gcatggacgt actcctttta cttcacataa tttttaacat ttcaatcact
     1801 actattttta ccacaaaata acgtcattcg tcttaaaatt caattgaata attgtcgttt
     1861 tgactttaaa ataaaacaag gtaaattaaa acgcttacaa gaaacgacaa atcattttta
     1921 aatttagtat atttctttgt ataaaattag catattctga tatgatacaa gtgttgcttt
     1981 tataaatttg aaaggatgta aaaccttatg acaataggta tcgataaaat aaacttttac
     2041 gttccaaagt actatgtaga catggctaaa ttagcagaag cacgccaagt agacccaaac
     2101 aaatttttaa ttggaattgg tcaaactgaa atggctgtta gtcctgtaaa ccaagacatc
     2161 gtttcaatgg gcgctaacgc tgctaaggac attataacag acgaagacaa aaagaaaatt
     2221 ggtatggtaa ttgtggcaac tgaatcagca gttgatgctg ctaaagcagc cgctgttcaa
     2281 attcacaact tattaggtat tcaacctttt gcacgctgct ttgaaatgaa agaagcttgt
     2341 tatgctgcaa caccagcaat tcaattagct aaagattatt tagcaactag accgaatgaa
     2401 aaagtattag ttattgctac agatacagca cgttatggat tgaattcagg cggcgagcca
     2461 acacaaggtg ctggcgcagt tgcgatggtt attgcacata atccaagcat tttggcatta
     2521 aatgaagatg ctgttgctta cactgaagac gtttatgatt tctggcgtcc aactggacat
     2581 aaatatccat tagttgatgg tgcattatct aaagatgctt atatccgctc attccaacaa
     2641 agctggaatg aatacgcaaa acgtcaaggt aagtcgctag ctgacttcgc atctctatgc
     2701 ttccatgttc catttacaaa aatgggtaaa aaggcattag agtcaatcat tgataacgct
     2761 gatgaaacaa ctcaagagcg tttacgttca ggatatgaag atgctgtaga ttataaccgt
     2821 tatgtcggta atatttatac tggatcatta tatttaagcc taatatcatt acttgaaaat
     2881 cgagatttac aagctggtga aacaatcggt ttattcagtt atggctcagg ttcagttggt
     2941 gaattttata gtgcgacatt agttgaaggc tacaaagatc atttagatca agctgcacat
     3001 aaagcattat taaataaccg tactgaagta tctgttgatg catatgaaac attcttcaaa
     3061 cgttttgatg acgttgaatt tgacgaagaa caagatgctg ttcatgaaga tcgtcatatt
     3121 ttctacttat caaatattga aaataacgtt cgtgaatatc acagaccaga gtagtcggtg
     3181 tatttaaaac acatataata aaacctaaaa gcagcagtaa gaccacttct aattgaaatc
     3241 gtcttactgc tgttctctat ttataacact tcgtattgaa tgaattcatt atgcctattt
     3301 gacacattat tgaagttttc ctaatgcctg gatcctttat acgttacggc ttcgtgctat
     3361 gttttggtac ataaagcttt gacatatcga tattctccaa ctctaacagc ttaattttat
     3421 tattaatcgt tccaccgaac cctgttaagc tacccgtttt accgacaaca cgatgacatg
     3481 gcacgataat agataatgga ttacttccga ctgcacctcc aaccgcttgg gctgacattt
     3541 ttggcttgtt aagcagcttg cctacttttt tggcaatagc accatacgtt gttagagtcc
     3601 cataaggaat ctgtcttaat tcattccaaa cacactgttg aaaatgacta cctgttggct
     3661 ttaaaggtat tgtgatttca ggattgtcac ctttaaaata cgcgtctaac cactgtgtcg
     3721 cctctctaaa tatcgctaaa gacgtatttt cttccctagt accatcacct tgttgatttt
     3781 caaacaaaac agcggtcaga cttaccccat cactcaaaag ttccaatcgt cctacaggcg
     3841 aatcatagta actcttatac tccataaaaa ttcccccttt ttctaatcat acgccttaat
     3901 tgttaaatta gaaaatattc atgttcacat tttttaaaaa taaataagtc gtcaattacg
     3961 tatataaaca cgtaatacca gctatcactt tgctgcaata tacagttaca tatcttacta
     4021 cacgtgctaa cctcttactt tgtaaaccaa atcttaaatt aaaatattga aaatgcaatg
     4081 aatccttaat attttattaa acctataatt acttattaaa aataacacac aatattcata
     4141 aagttttaaa aatattctgt tttatcacct actattagtg gaaaagtaca attgcaattg
     4201 tatatagttt gcataacgct tcaaaagtaa tttctttttt gtttagttca aaaaaattta
     4261 gaggtgatgt tatatgaata acggtttttt caatagcgac tttgattcaa tttttcgaag
     4321 aatgatgcaa gatatgcaag gttcaaatca agtcggaaac aaaaagtact atattaatgg
     4381 taaagaagtt tcacctgaag aactagcgca actcacacaa caaggtggca atcactctgc
     4441 tgaacaaagt gcgcaagctt ttcaacaagc agcacaaaga caacaagggc aacaaggtgg
     4501 caacggcaat tatttagaac aaattggtcg taaccttacg caagaagcac gtgacggttt
     4561 attagatcca gtcattggtc gtgataaaga aattcaagaa actgctgaag ttttaagtag
     4621 acgaactaaa aacaatccta tattagttgg agaagctggt gttggtaaaa ctgcgattgt
     4681 tgaaggttta gcacaggcaa tcgttgaagg aaatgtacca gcagcaatca aagacaaaga
     4741 aattatttct gtagacattt catcattaga agctggaacg caatatcgtg gtgcttttga
     4801 agaaaatatt caaaaattaa tcgaaggtgt taaatcttca caaaatgccg tactattctt
     4861 tgatgaaatc catcaaatta tcggttcagg tgccacagga agtgattcag gtagcaaagg
     4921 gttatctgat attttgaaac ctgcattaag tcgtggtgag atttctatta ttggtgcaac
     4981 aacacaagat gaatatcgaa acaatattct taaagatgct gcattaacgc gcagatttaa
     5041 tgaagtgctt gttaatgaac caagcgctaa agatactgtt gaaattttaa aaggtattcg
     5101 cgaaaaattc gaagaacacc atcaagtaaa attaccagat gacgtattaa aagcatgtgt
     5161 tgacttatca attcaatata ttccacaacg attattacca gataaagcaa tcgatgtgtt
     5221 agatattaca gcagcacatt tatctgcgca aagtccagct gtcgataaag ttgaaactga
     5281 aaaacgaatt tctgaattag aaaatgataa acgtaaagca gtaagtgctg aagaatataa
     5341 aaaagctgac gacattcaaa atgaaatcaa atcattacaa gataaattag aaaatagtaa
     5401 tggtgaacat actgctgttg ctacagttca tgatatttca gatactattc aacgattaac
     5461 tggtattcca gtttctcaaa tggatgataa cgatattgaa cgtttaaaaa atatttctaa
     5521 tcgtttaaga agtaaaatca taggtcaaga tcaagctgta gaaatggttt cacgtgcaat
     5581 tcgccgtaat cgtgctgggt ttgatgacgg caaccgtcca attggcagtt tcctatttgt
     5641 tggccctact ggtgttggta aaacagagct tgctaaacaa ttagcaattg atttatttgg
     5701 taataaagat gcactgattc gacttgatat gagtgaatat agtgacacaa cagctgtttc
     5761 aaaaatgatt ggtacaactg ctggttatgt tggttatgat gacaattcaa atacgttaac
     5821 tgaaaaagta cgccgtaatc catactcagt cattctattt gatgaaatcg aaaaagcaaa
     5881 tccacaaatt ttaacattgt tattacaagt aatggatgat ggtaatttga ctgatggtca
     5941 aggtaatgtc atcaacttta aaaatacaat tattatttgt acatcaaatg ctggctttgg
     6001 caatggcaat gacgctgaag aaaaagatat tatgcacgaa atgaaaaaat tcttccgccc
     6061 tgaattcctt aaccgcttca acggcatcgt tgaattctta catttagata aagatgcatt
     6121 gcaagatatc gtcaacttat tattagacga tgtacaagtt acattagaca aaaaaggtat
     6181 tacgatggac gtttctcaag atgcgaaaga ttggttaatt gaagaaggct atgatgaaga
     6241 attaggtgca cgtccattaa gacgtattgt tgaacagcaa gtacgtgaca aaattacaga
     6301 ttactattta gatcatacag acgttaaaca tgtggatata gatgttgagg ataacgaatt
     6361 agtcgtaaaa ggtaaataac gacactttaa catatcgcgc atcaaaaatg agcatcaggt
     6421 cgcccttgcc tgtgctcatt tttttaatta tttccctgga aaatgattcg ctgtgtgctg
     6481 ttctgttcca caacaatcac gattaatgtc acatgtacca cattttcctt gttttgaacg
     6541 cttgaaaaat tttactagtg tatataaggc atatccgaaa attgctaaaa aaattaaaat
     6601 gttaataatg actgacactt taaccactcc ttaaacaaat aaatgtccga cttgataaaa
     6661 aatgaatgtt aagacatatg cagtgactag aggataggca actgcaagtg ccgtccattt
     6721 ccatgaataa gtctctttac ggattgctgc tactgtagaa acacaaggaa tatacaatag
     6781 tataaatatc ataaatgcat acgcagatag cggtgtgaat tgattttgaa tcacattaac
     6841 aaggcctgca tcacctgatg aatagataat cgccatcgaa cttacgataa cttcttttgc
     6901 taaaaatcct ggcactaacg tagcacctgc ttgccatgtt ccaaatccga gcggttgcac
     6961 taacatacca aagaaactac caaccatatg taaaaaactt tgattgatat tcacattgat
     7021 accatgtggt cctacataac ttaatagcca aatgactact gagccgccaa aaataaatgt
     7081 acctgcttta cgaacaaagc ccttagcctt ttcccaagta ctacgccaca acgttttaat
     7141 ggaaggcaca cggtatgttg gcaattccac aataaagatt gcattatcat tttttaaaat
     7201 cgtcttagta agtactgtac tgactaaaaa tgccataata atacctaaaa catacaggct
     7261 taatactact aaagattgat tctctttgaa aaagatacct acgaacaacg catacactgg
     7321 cagtctagca gagcatgaca tgaatggtgc aattaatatc gttgttaaac gctctttttc
     7381 attttcaata ctgcgcgcag ccataatact cggtacatta caaccaaatc cgataatcat
     7441 tggtataaaa gacttcccgc ttaaaccgaa cgattccata atacgatcca ttaaaaccgc
     7501 aatacgtgcc atataacccg aatcttctaa taaagatata aagaaaaaga gcacaacaat
     7561 ttgtggtaca aagactaata ctgatcctac accagcaata atgccatctg taattaaatc
     7621 ttgtaaaaat ggtataacac caagataatt cataatcgtc ttcacactat ctgtaaatgt
     7681 accacctata aatgcatcga gttgatccga caaaggtgtg ccaatccatg taaatgtagt
     7741 ttgaaagatc aaccacataa ttgctagaaa gataggcatc cctatatatt tatgtgttaa
     7801 tatcttgtct attctagagc tgaaatattg cttatcttca tctggatacg ttaccacgtc
     7861 ttgcaataac gtctcaatat aatgattgcg tatacgctcc atctctcgac gaacagatac
     7921 agcccctact tgttcagcaa cttgatcacg taaactcgac aatttattta caacctctga
     7981 attaagttcg tttgcaattt gcatgttatt taataagaat tgaatcgcaa taaacctagc
     8041 ttgatactta tcatgagatg tctctgtcat tattatttga cacatatttt taattgtctc
     8101 ttcaatcttc tcaccataat tgattttaaa atgcggttga tacccttccc ctagatgctt
     8161 tatttcgcca agtaaatatt ttgttccttt gcctgtacgt gccacaactg gaaaaatagg
     8221 tgtttttaac tttttcatca atttatgata atcgattttt atcccgcgct ttgtagctac
     8281 atcaatcata tttaatccga tgtatattgg ttgattaagt tctaacaatt gtactgttaa
     8341 ttgcatattt ctttttagtt gactcgcatc aacaatgtta atgattcctg aaaatgaatc
     8401 gtttaataaa tagtctgtca ctacagtttc atctttagaa atcggcgata aatcatatgt
     8461 acctggtaaa tcaattaatt gtcctacatt ttctttaagt ttccctactt ttttctctac
     8521 cgttacgcca ctccagttgc ctatatattc atacgaacca gttaaagcgt taaacaaaga
     8581 tgttttacca acattaggat ttcctaaaat acaataattt tccattcgtc cggctcctat
     8641 tcttctaatg caatagaaca agcatcgcaa tgtctaatac ttaactgttg tccgtttact
     8701 tcaataatac atggcccttt aaataaacat ttttgtttaa tcgttatgat agcgtcatct
     8761 gttaacccaa aggcacttag acgatacaac atattctcat tagcaatatc cattcgcttt
     8821 attttataag ccttattcat ttcaccattt ttaatgttta acatactatt tcgctctcct
     8881 attagaaata ataatcatta tcacttaaaa atcataaccc ttaaaattgt agctcgcaat
     8941 actttattta aataattttc atttttcatg taaaatttgt gacattgcaa aaatgttttg
     9001 tataatcact tccatgcaaa aaacctccgc ttcaaatgtc attgatagac atttaaagcg
     9061 gaggcatgta catgttattt tatgatttat ttcgtagatt tttttctaac aattgattga
     9121 gcttttcaat atttttattc aactgttcta tattcgttgc ttgttgtttt gaatttagtt
     9181 gatgcttgtt ttgataatct attaaagcat catataagaa caatgaactt gctgattgct
     9241 gtttcacttc ttggtacaat gccatcgttt tatcatctag tactattgaa tgtgcatcat
     9301 cttgcccatg tgcatcttga tgtttcgata catttgttgc accttgtgga tatactttaa
     9361 aggttgtgtc gtatgtttcc ttactatctt tagcttcaga ttcctgtgat tcaaccgttt
     9421 tatatttttc aagtgcatgt ccttcaatat caactcgtgg aataatgcga ttcaaccatg
     9481 ctggtaaata ccacgaacct tttccaaaca atttcgttaa tgcaggaatt aacatcattc
     9541 tgactacgaa ggcatcaaag agtacaccaa acgctaatgc catacccatt gatttaatca
     9601 tgacatcttc ttggaataca aacgcaaaga agacactaaa cataattaat gcagctgcta
     9661 caataacagg accgctttct ttcaatccta ctttgataga ataatcatta tcccctgttt
     9721 tactatattc ttcatgaatt cgcgacataa ggaagacttc ataatccatc gctaatccaa
     9781 ataagatacc tatagtaata accggtaaaa atgctagcat tggtcctgtc gtttcaatac
     9841 caaacagacc tttcataaaa ccatcttgca ttactaatgt tgtaaatcct aatgttgcca
     9901 ttaatgacaa gacgaatcct aaaactgctt ttaatggtat tagaattgaa cggaagacaa
     9961 tcattaataa gaaaaatgct aatacaacaa tgactgaggc aaataaaggt atcgcctcat
    10021 ttaacttttt agacatatca atattaatga cactttgtcc cgaaatctcc gttttgaacc
    10081 catatttatc ttgtgcatct ttatgataat ctcgtaaatc atgcactaaa tcatttgtac
    10141 tctctgcatt aggcccttgc ttaggtatca cgaccatcaa agcgtaatca ttatctttac
    10201 tcatttgtgg tggcgtaacg atatctacat ttttcttatc tttaatatct ttatatacag
    10261 actgtaaatc ttgttgtaat ccttgtggat catccttttt atctttcaca tttatcaaca
    10321 tcggtatttg gccattaaat ccttcaccaa atttatccga gataatatcg taagcttttt
    10381 tctgtgtaga atctgctggt ttaacaccgt catctggaat accaagtcgc atatgactaa
    10441 ctggtattgc agctgctact aatatgatta aacctagtaa tactgccgca agtgcatttc
    10501 ctgtaataaa tttagaccat ggcgtatcaa tatctttttt gaatttagac tgtaatttat
    10561 tcactttaat gcgtttatgg aaaatgctta ttaatgcagg taataaagtt aaagcgctaa
    10621 gtactgcaaa aacaacacta attgccgaag caaatcccat taccgctaag aagtcaatgc
    10681 ctactaatga taaaccacat actgcaatta caactgttac accagcaaaa acaactgcac
    10741 tacctgctgt tcctattgca agaccaatgc ctttaatgta atctgtttca gttttcataa
    10801 cttgtcgata tctgaataaa ataaataatg cataatcgat accaactgct agtccaatca
    10861 ttacggctaa tgtcagtgtg acatttggta tatcgaatgc ataagttaac aaactgataa
    10921 tacctacacc agaggctaga ccaatcaatg cacttataat tggtaatcct gcagcaatga
    10981 ctgaaccgaa tgtgattaac agtacaacaa atgcaacaat aataccaact agttcagaat
    11041 taccgcctac ttctgtagat gtcattcctg taccagttaa ctctgtttgt acattatgat
    11101 tgtcacgtaa gtctttcaaa tgacttttaa cattatctct agagccatct tttaaagatg
    11161 tttgactaac gtcatatgtg atatctgcaa atgcagttgt tttatcttta ctaatttgct
    11221 tattttcata aggatctgat attttatcaa tgtgcttgtc atctttttta atatcatcta
    11281 acgttttctt aatatcttta gtaatgttcg gttgcacaat accatcatct ttagtcgtct
    11341 taaagacaac acgtatttgt gccttttcac tatcttgatt aaaatgtttt tcaatctttt
    11401 tattcgtatc taacgactct aatcctgtca ttttaatatc attgtcaaat ttcggtgcat
    11461 ttgtagcaag tggtatcaat attgcagcta caatcactat ccatgcaatg accgcggacc
    11521 atttatgttt tgcgatgaat gtccccatct tatataaaaa ttttgccaaa gtatattgcc
    11581 tccttttaaa atcaacgtta tagtttaaat atacagtgta gattattgtt cgattatagt
    11641 atctatcccc gacctcttaa agaatcaatt ggaaaatttt gtatattaaa ctacacacaa
    11701 aggagaaatg tagatgaaag agactgattt acgagttata aagacaaaaa aagcattgtc
    11761 gagtagcttg ctacaattgt tagaacagca attattccaa acgattactg tcaatcaaat
    11821 ttgcgacaac gcactcgtac accgtacaac attttataaa catttttatg ataaatatga
    11881 tcttctagag tacttgttca atcaattgac taaagactac tttgctagag atatcagtga
    11941 ccgtcttaat catccattcc aaacgatgag tgatacgatt aataataaag aggatttgag
    12001 agaaatcgca gaattccaag aagaagacgc tgaatttaat aaagtattaa aaaatgtctg
    12061 cattaaaatt atgcataacg atatcaaaaa taatagagac cgtatcgata ttgacagcga
    12121 catcccagat aatctcatat tttatattta tgactcgttg attgaaggtt ttatacattg
    12181 gataaaagat gaaaaaattg attggcctgg cgaagatatt gataacattt tccatagatt
    12241 aatcaatatt aagattaaat agtagatgag aaactcatga gcgttaccaa cattcataat
    12301 aaaaacgata gtgtacacgt taatgaattc gtgtactact atcgtttttt atttttatcg
    12361 tgcttatcgc tattaaaaca actgatacac aacacataaa ctatgaagaa aaaaataaat
    12421 ccgctatcta aatgacttta actcagttgt ttaaatgacc aaattgctaa tacaattccc
    12481 attattattg aaataacgta tctcacattc ttatacctat aatccttttc taaaaatatg
    12541 gttgctatta cttaattttt aaagttataa ataaaaagag ccaaccgcaa tggatggccc
    12601 ttgttcatta tgaagcatta gaacatttct gaaacaacct tttgttctaa gaagtgtaat
    12661 aagtagtctg gactacctgt tttagcgtcc gtacctgaca ttttgaaacc accaaatgga
    12721 tggtatccaa caactgctga agtacagcct ctgttaaggt ataaattgcc tacatcaaat
    12781 tcgtttaccg ctttaatcca atgctcgcga ttatttgtaa tcactgcacc agttaaaccg
    12841 taatctgtat catttgcaac ctcaattgct tcatcaaaat cgttaacttt cacaaagcca
    12901 acaactggac caaaaatttc ttcttgcatg attctatctt tagatttaag tcctgaaatg
    12961 attgttggtt ctacaaagta accttttgaa tcatcagtgc cgccaccttg ttctaattta
    13021 ccttcttctt taccaatctc aatataattt ttaatcttat caaattgttt tttattaata
    13081 actgggccca tatacgtatt gtctacagta ttgcccaacg ttaattcttt tgttaatttg
    13141 attgatttct ctaatacttc gtcataaacg tctttatgca caattgcacg tgaacatgct
    13201 gaacattttt gaccagaaaa accaaatgct gacgttacaa tagcttctgc tgccatatct
    13261 gtatcaatat tttcatcaac tacaatggca tctttaccac ccatttcagc gataacacgt
    13321 ttcaagaagt tttgaccttc ttgaacaacg gcactacgtt cataaattct agtacctgtc
    13381 gcacgtgatc ctgtaaatgt aacgaaatgc gtatctttat gatcaactaa gtaatcacca
    13441 atttctttcg gatcaccagg aacaaagtta actacgcctt ttggtaatcc tgcttcttct
    13501 aaaatttcca ttaatttata agcgatataa ggtgtatcct cagcaggttt caataacact
    13561 gtattacctg ccacaactgg tgctaaagtt gtaccagcca taatcgcaaa cgggaagttc
    13621 cacggcggaa ttgtaacacc tgtaccaatt gatttataga aatatttatt gtgttcacct
    13681 tcacgatcaa gtactggctt accttgagcc aagtccatca ttgaacgtgc atagtattca
    13741 ataaaatcaa taccttcagc tgcatcacca actgcttcat cccatggctt acctgcttca
    13801 taaaccataa ttgctgcaat ttccgctttt cgacgacgaa taattgccga aacacgtaac
    13861 ataagctctg cacgatcatt tgctgaccat gttttccaag atttataagc ttcgtttgct
    13921 gctttaaacg catcttcaac atcttgtttt gttgcctttg atgcatttgc aatcacttgt
    13981 gatgtgtctg caggattgat tgatttaatt ttgtcatctt tgaaaatctt ctctccatta
    14041 atcactaatg gtatgtcttg acctaattct ttttccacgt ctttcaatgc tttcttaaac
    14101 atatccacat tttcttggac tgaaaaatcg taaccaggtt catttttaaa ttctactacc
    14161 atgtacactt accccctata aattttgaaa gtggtttaac cctttgattt aatgatataa
    14221 catcatttaa actcatttta ctatgattaa ggttagtttt gcaaccgctt tcatttttat
    14281 gttttatcac ttattctcaa gtattttgaa attgattggt tactttttaa aatttatatg
    14341 ggtcgcaact gctactttat cgtttcgtca tttaatgttt cggatggtag gtcattatca
    14401 attttacgaa cgactttaca agggtttcca accgctaagc tgtgtggcgg aatatcttta
    14461 gtgacaacac taccagcacc aatcacactg ccttctccaa tcgtcacccc tggtaacacg
    14521 gctacatgac cgccaaacca agtattactg ccaatatgaa tgggtccggc tttttcaaaa
    14581 ccttcatttc tatgatggaa attaagtgga tgtgtcgctg tgtagaatcc acaattaggt
    14641 cctataaaaa cattatcgcc aattgtaatc tgtccaccat ccataaagta acaattggtg
    14701 tttacataga cattttttcc tagtttaacg ttccaaccat aatctgtatc aaaaggaatc
    14761 gaaatactta cattgtctgt tgttgtttga aataattgat caattaattc ctttctttta
    14821 tttgtagcac tcggtcttgt atgatttaat tcaaagcaaa tatctttcgc tcgtgcacgt
    14881 tcattgatta agtattgatc aaagtttgca tcgtaccatt tttctgctaa cattttttct
    14941 ttttcagtca ttacaccttt caactcctaa taacttattt acttgtttaa aagttaatca
    15001 aataaacctt cgcctatgca actaatacgc tataacatta tgaaatcatg accttatcac
    15061 ccttatctat acaattctcg catcaaatac tgctaaagta gtagataaat tcaatactac
    15121 agacgcattc attttttaat ctattaacgt acaatgtgag taagagaaat ataaaggagt
    15181 atgatagcga tgagaatatt aattacaggc acagttgcta tcttaatcat tctaggtttg
    15241 gtcaaaacga tacaagatta cgaaatgaca aacgacacga gtcgtcagtt gtcagacaac
    15301 aaagatgatg ataaagtcat ccatcttaat aattttaaaa atttacatgc gaaagaattt
    15361 aacccatctg atttctttta agtcacctaa gaattgcaaa tccagaagtc atttaagttt
    15421 tacctttcat tcatacatcc tttaatatta attacgactt cttttatata gatgctaagt
    15481 agagagattg ttgtgcaatg tttgcacggc aatctctctt tttcttttta aaattggtaa
    15541 aagtaaaacg caacgattga cttatatacc tatagggggt acattagacg tgtaacaatg
    15601 aatcacaggg aggcaataat gtggctaata cgaaaaaaac aacattagat atcactggta
    15661 tgacttgtgc cgcatgttca aatcgtatcg aaaagaaact gaataaactt gatgacgtta
    15721 atgcccaagt gaatttaact acagagaaag caactgttga gtataaccct gatcaacatg
    15781 atgtccaaga atttattaat acgattcaac atttaggtta cggtgtcgct gtagaaactg
    15841 tcgaattaga cattacaggt atgacttgtg ctgcatgctc aagccgtatt gaaaaagtgt
    15901 taaataaaat ggacggcgtt caaaatgcaa cggtcaattt aacaacagag caagctaaag
    15961 ttgactatta tcctgaagaa acagatgctg ataaacttgt cactcgcatt caaaaattag
    16021 gttatgacgc gtctattaaa gataacaata aagatcaaac gtcacgcaaa gctgaagcgc
    16081 tacaacataa attgattaag cttatcatat cagcagtatt atctttacca ctattaatgt
    16141 taatgtttgt acatcttttc aatatgcata taccagcact atttacgaat ccatggttcc
    16201 aatttatttt agctacacct gtacaattta ttattggatg gcaattttat gtaggtgctt
    16261 ataaaaactt aagaaatggt ggcgccaata tggatgtact tgttgctgtt ggtacaagtg
    16321 cagcatattt ttacagtatt tatgaaatgg ttcgttggct aaatggctca acaacgcaac
    16381 cgcatttata ctttgaaaca agcgccgtac taattacctt aatcttattc ggtaagtatt
    16441 tagaagctag agcgaagtct caaacaacca atgcgcttgg cgaattatta agtttacaag
    16501 ctaaagaagc acgcatttta aaagatggta atgaagtgat gattcctcta aatgaagtac
    16561 atgttggaga tacacttatc gttaaaccag gtgaaaagat acctgttgat ggcaaaatta
    16621 ttaaaggtat gactgccatc gacgaatcta tgttaacagg tgaatctatc cctgttgaga
    16681 agaatgttga tgatactgta attggttcaa cgatgaacaa aaacggtact attactatga
    16741 cagcaacaaa agttggcggg gacactgcgt tggcaaatat tattaaagtt gtcgaagaag
    16801 ctcaaagttc taaagcgccg attcaacgat tggcagatat tatttctggt tatttcgttc
    16861 ctatcgttgt tggtatcgca ctattaacat ttatcgtgtg gattacttta gttacaccag
    16921 gtacatttga acctgcactt gttgcgagta tttccgttct cgtcattgct tgtccatgcg
    16981 cattgggact tgctacacca acttctatta tggtaggtac tggtcgcgct gctgaaaatg
    17041 gtattttatt taaaggtggc gagtttgttg aacgcacaca tcaaattgat accatcgttt
    17101 tagataagac gggtaccatt acaaatggtc gtccagtcgt gacagattat catggtgaca
    17161 atcaaacgct acaactactt gctactgctg aaaaagattc tgaacaccca ttggcagaag
    17221 ccattgtcaa ttatgcaaaa gaaaagcaat taatattaac tgagacaaca acatttaaag
    17281 cagtacctgg ccatggtatt gaagcaacga ttgatcatca ccatatattg gttggtaacc
    17341 gtaaattaat ggctgacaat gatattagct tgcctaagca tatttctgat gatttaacac
    17401 attatgaacg agatggtaaa actgctatgc tcattgctgt taattattca ttaactggta
    17461 tcatcgcagt ggcagatact gtcaaagatc atgccaaaga tgctataaaa caattgcatg
    17521 atatgggcat tgaagttgcc atgttaactg gcgataataa aaacactgct caagccattg
    17581 caaaacaagt aggcatagat actgttattg cagatatttt accagaagaa aaagctgcac
    17641 aaattgcgaa actacagcaa caaggtaaga aggttgcgat ggttggtgac ggtgtaaatg
    17701 atgcacctgc attagttaaa gctgatatcg gtatcgccat tggtacaggt acagaagttg
    17761 ccattgaagc agctgatatt actattcttg gtggcgactt gatgcttatt cctaaagcca
    17821 tttatgcaag taaagcaacc attcgtaata ttcgtcaaaa tctattttgg gcattcggct
    17881 ataatattgc cggtatccct atagctgcat tgggcttact tgcgccatgg gttgctggtg
    17941 ctgcaatggc actaagttca gtaagtgttg tcacaaacgc acttagattg aaaaagatgc
    18001 gattagaacc acgccgtaaa gatgcctaga ttccttaata atgaaggatt cgttggtgat
    18061 tctgagatag gctagtgatt ggctctataa tgtcgcggtt tacagttgga tcttcgctcc
    18121 aactgcataa gagccactaa ttattataaa taataagtgg ctctaaaagg aggtgaatgc
    18181 tatgtcacaa gaaattttaa atgttgaagg tatgagctgt ggtcactgca aaagtgctgt
    18241 tgaatctgca ttaaataata ttgacggtgt cacttcagct gacgttaacc ttgaaaatgg
    18301 tcaagtaagt gttcaatatg atgacagtaa agttgctgta tctcaaatga aagacgcaat
    18361 tgaagatcaa ggttacgatg tcgtttaatt aggcaatatt caacgtcatc aacaccaaat
    18421 taaaaaatcg aactgatgag aatcccaaca atccaaatta tctcatcagt tcgattttta
    18481 atttactcgt aacctagtat ctccagtctg caatacatct aatgttgcat ctaatgcatc
    18541 gacaattaga tttttaactg cagcttcagt ataaaacgca atatgtggtg ttaatatgac
    18601 atcttccctg tcaatcaacg attctaacaa tggatcgttc agtgttttgc ccctttgatc
    18661 acttgggaaa agtttgcgtt caaattcata cgtatcaagt gctgcacctt taatcacacc
    18721 attgtctaat gcgtctaata acgccttagt atctactaaa caacctctcg cacaattgac
    18781 aaatactgcg ccctttttaa aatgtttaaa taattcagca ttaaatagat aatgattata
    18841 tttcgttgca ggtacatgta atgtcacgat atcagcacct tcaaccgctt cctcaatcgt
    18901 atctttgtaa tcgacatacg ttgcaatttt agcattagga aacgggtcgt atgcgaccac
    18961 atcactttga taaccattgg caaatatatc ggctactaca cggccaattc gacctgtacc
    19021 aataacagct acttttaaat ctttaatgga tttcgataaa atagtaggtt cccatctaaa
    19081 atcatgctcc cgcactttcg tttgaatttg attaaaatga cgaaccacat taatagcctg
    19141 gttcacagca aactccgcaa ttgaattcgg agagtatgac ggcacatttg acacaataaa
    19201 gttatacttg tttgctaact ccaaatcata tgtatcaaat ccagcactac gttgtgcgat
    19261 ttgtttaata cctagttcat ttaatcgttt ataaacatgc tctgataatg gtatttgttg
    19321 tgatagcgat aagccatcat aaccagcgac accttcaaca ttgtcatcag ttaatgcttc
    19381 tttagtaata tctacctcaa catgatgttt ctctgcccac gccttgatat aaggcatatc
    19441 ttcatcacgt acactcatga ttttaatttt tgtcatttta acatcaccct taactttatt
    19501 attcatataa atatgctagt tctgttaatc ttattgcagc ttcgtctaat ttctggtcat
    19561 ctaacgccaa tgaaattctc acataacgat taccattctc tccaaatggt ttccctggag
    19621 caacaagtat tgacttctct tgcactaaaa attgctcaaa ttgctcgctg tcataaccag
    19681 gcggtgtttc caaccataca tatatgccac ctttagcatg aacaaatggc aaatcagctt
    19741 ttgcaagcat ggcttcgaat cggtcacgac gtgttttaaa tacattgctt tgttcttcta
    19801 aaaaatcatc ataatgattc aaagcatata ttgcggcatc ttgtaatgca ccaaacatcc
    19861 cagcatttgt gtgcgtttgg tactttttca aagcttgaat catatcttta ttaccaactg
    19921 caaaaccgac tctaaaacct gacatgttat aacctttaga caaggaataa atttcaatcg
    19981 caacatcttt cccattttcc gaagcaagta tactaggatt tttagcgtcg aaaccgaaag
    20041 caccataagc aaaatcatgc acgattttag tgtctgtacc tttaaattta gctatcgctt
    20101 catcaaaaac ttctttcgta gctgtcgatc cagttggatt atttggatac gttaaataaa
    20161 tgagttttgt tttatctatt atttgtgaat caactttgga ccaatctggc aaataatgtg
    20221 gcggttctaa attaagcggg actggcttgc catcagctaa aagtacacct gctaaataat
    20281 ccgtgtagcc tggatcaggt agtaatacat agtctcctgg attgataaca catgttggta
    20341 ctgccactaa tccatttttt gtaccatata aaatgcatac ttcatcttct ttatctaacg
    20401 tcacattata ttgtctttga taaaaatcta caatagcttg cttgaacgct tctttaccat
    20461 gaaaagcacc atatttttga ttttcaggaa tagttagtgc tttttgaaaa tgatcaataa
    20521 taccttgtgg cgtgggccca tcagggattc caactgccat attaattaat ggcaatggtc
    20581 catgttcgat tttacgtccc atcgttttcc cgaaataact atcagggata tttgctaatt
    20641 tgttagagat catcaaattc ctcctctatc attaaacata gcctgggcga ctatcataat
    20701 cctaacaact tgtatcactc tcatttagat ggttacaatg acatcgccat tcaccgttat
    20761 gttcaacaga acttatgaca cacgttgtat tgaatgaatt tattttcatt ttaggtaggt
    20821 ataatattat tgtcaatatt aggaattttc agattaatat gcactcaatc gttatgattt
    20881 aactgtcatg catatccgca tgcgcaacca gttagatatg cttatataaa gtataacgcc
    20941 catcaaggta cgtattcaaa cgtgaacctt aacaggcgtc attcattgtt aaataaaact
    21001 tcttaagcac atacttattt cactatgcct tttacgttcc ccttatactt ttctcacatc
    21061 tttctcttag actactccct tatacgcccc gctcaatatc tttaatcatt tcatctacag
    21121 ttattttcgc actcgttaag acaataggaa cgcctgcacc tggatgcgta cttgcacctg
    21181 caaaatataa atctttataa tctcgcgata cattttgtgg acgataataa ttactttgcg
    21241 ctaaagttgg cattaaaccg aatgccgaac caaatttcgc atgatacgtt tgctcaaaat
    21301 catttggcgt aaagattgtt tctgaaacaa tatgcgattt tatatcttca aatacttcaa
    21361 tcgttgctaa tttacgataa ataatttcct ttatttgttg cgtcaaagct tcatctgacc
    21421 aatcgattcc gctacctgtt ttaagttccg gcgtcggcat tagcacataa ataccagttt
    21481 tgccttctgg cgcaagtgat ttatcagcga ccgctggtac atacacataa atagaaggat
    21541 catatgataa acgtccctca aatatttctt caatattgcc tctaaagtca tctgaaaaaa
    21601 taacattatg aagtctcact tgatctgtca catcaatatc tataccgata tacattaaaa
    21661 atgctgaaca agagtaatct aagtctgcaa ttttatgtgg tggatacttt ttaataggtg
    21721 caaaatctgg cattaatgat tccgcaacac tagggaaatc agccgtacat aaaattttat
    21781 caaattttct tatgtcacca ttcactttta tcgcatcggc ccgtttgaat ttaggatcaa
    21841 taataatttg ctcaatttca gcatttagtt caatattaac gcctaagtct ttatttaatt
    21901 gcgctagccc ttgagccatg ccatacatac cgcctttaat aaaatgcaca ccaaacatca
    21961 tttcaatcat aggaataatt gaatatagtg acgggcctcg ttttggatca attcctatgt
    22021 ataacgtttg aaacgctaaa agcttttgta tcttttcgtt atcaatataa tgttcaatta
    22081 gctgatctgc atgatttaac gtttttaact tagcaccttg cacaagtgac gtcatattat
    22141 aaaagtcact cggtttgcga tacgttcttt ctaagaaata gcgacgtgca atttcatatt
    22201 ttttataaac atccgttaaa aaggacataa aaccatgcgt tgaaccaggt tctatacttt
    22261 ctagcatttg ctgtaattca gctaaatctg taggcaccgt tatacgatca tcgtggtcaa
    22321 aatacacatc gtaaatataa cgtaattgtc tcaattcaat ataatcttca taatttttac
    22381 cacacgctgt aaaaacatct ttataaacat ctggcatcat gacaattgtg ggacccatat
    22441 caaatgtaaa gccgtctttc tttaattgat tcatacgccc gcctacatta ttattttttt
    22501 caaatatcgt cacttcatga ccttgagaag caatacgggc tgccgctgct aatcctgtga
    22561 cacctgcacc aattactgca atcttcatta ttcaaccacc tatattctat gatatttact
    22621 atttatttca tgaaacaact ttgccttttt cctcttatcc acaaaaacac gttcatgtaa
    22681 tgtatagtta gcctgtctca cttcgtccag tatttcaata tatatacgtg ctgctaattc
    22741 tatgattggt tgtgcttcaa tactaaatac tttgatttga tccataacat cttgaaaatc
    22801 tttttctgcg atagctgcat aatattccca taagtcaata taatgattat taacaccatt
    22861 ttggtacact tcagcaatat caacttcata ttgctttaat cgttgcttac taaaatatat
    22921 ccgttcattg tcaaaatctt caccgacatc tcttaatata ttaatcaatt gcaacgattc
    22981 accaagtctt cttgcgacat cgtatgtctg atgtgtttca tgatcactta aaatcggcgt
    23041 caatacttca cctactgtac cagcaacacc ataacaatat ccgaataatt cagcgtccgt
    23101 ttcaaacatt gtaaaatgtt gatctttata tacagtatca atgagattat aaaaagattg
    23161 aaaggcgata tttttatgtt gtgcaacatg ctgaagcgcc atcatgattc tacgatcact
    23221 ttgaaagtga tgatgttcat atgggtattt ttcaatagat tgtatatctt cttttatttg
    23281 atttaaaaat tgaatatcgc cataaacatc tatactgtca tcaattttac gacacacagc
    23341 ataaattgcc caaaccgctt ttctttgatc ttctggtaac aagtcaaaag cgtaagaaaa
    23401 gctttttgaa tgtttcttca tgattttatg acaatattta aaattcatat ccatcattgt
    23461 catactagtc ctcctatatt gaaatgccat taccttgatt tattgttctt tagatataga
    23521 atattgacga cctttccatt caactacacc ataacgatgc gtttgtttcc aagattggat
    23581 gaaaatttta gtaaaaaaca taaataacaa tggatgacat accattaata aattagaaaa
    23641 ttggccgacc cttcgatcca gataaataaa ttgtgtagta tagctcaagt aaagtgctac
    23701 catttttctt acagacattt ggcgatattt taaactaaga catagcccta aaatactcgc
    23761 tatagaacca aacaaccaca acacaattgc tgtcatgatc ttaggctttg tgccacctgc
    23821 cccagttgac aaatgctttg tccatccttc ttgtaatgac tgaaatcctt cttgatacat
    23881 gcgaaatgca acaaatggaa acccttcata aactgttacg ggcaatgatt gtgaagtata
    23941 tgcacttcct aaagcaaatc cttcaataat atgacggttt gcacttttat gacctccagt
    24001 tgcataataa tcttctttat ttgttaatgt caccggtcca aatgcatgct ggttagtccg
    24061 accgtcgtct aaggtagaaa atacattcat accaacgact gtcattaaat taaatatcgc
    24121 tgaaaaccct tcgtagaaac gctttgttat atgataaggc tgtacgctta acaatccttt
    24181 ttcaccttgt aattgatact gattaatcaa cgtttcaaca gcatctttcc ttaagaaagt
    24241 tacatcagca tctacaaagg caatgcgatt cgtacatgca tgcgtcacac cttgataaca
    24301 agcatgtgat ttcccatacc atttgccgtc agtatcatca acaacatcta ctacagtcgc
    24361 accatatgaa cgtgctacac gtgctgtttc atctgtcgat ccgtcattca taacaatgac
    24421 atctactgga acttgctgtt gtattatcga atgtagtaaa tgacctattc ttttttcttc
    24481 gtttctagcc ggaataataa ttgttaatgc tttatgattg aagttcagcg ttttcgcctt
    24541 tagctgatgt ctacgattaa atatcaatgc accacacgcc atagacatgg tcactattac
    24601 tgttaatatt cgtgataacc atttcataga tatcacttcc tattcttcgc ttctcgcgcg
    24661 tttattttgt ctgcgacttg ttgcccactt aatgtaacca ttggcatgcc accaccagga
    24721 tttactgatc cacctacaaa gtacaagttt tcaaaatact gactttcttt aggaaattta
    24781 aatcctttgt ttttcttttt atctgctaca acaccatata ttgcaccacg attagaacga
    24841 taattttttt caatatcctc cggtgtccaa acatcttcat aaataatgtg ttttcttaaa
    24901 tcagtaagtc ccattttttc taatttatcc aaaattttat ccctaaactt cgcataatct
    24961 tcagtggtaa aaggctgatc ttgaatatat ggaatatgtg gtaagacttt gatattttca
    25021 taacctactg gcgcttgtgt gtgatcagtt ttattcgtat ttactagata aatggtcgga
    25081 tcatctggca ataccttttc atgaaaaact tgttgataat tgagataagc attttccgta
    25141 aaaaagaaat tatgatgtgc taattgcggg tattggcaag caacacctaa atgcatcaca
    25201 tatcctgagc ttgccggctc aaattccctc tctaatttgt ttaatcgttg agtatcaagg
    25261 tgaattaaat atttataagt aggtatgact tccatatttg aaataatata atctgccttt
    25321 acaaactcac ctgtatctaa tctgacaccc gtcacacgtc tttgatatgt tttaatattg
    25381 tccacacgtg cacctgtatg aattgtgaca ccttcttcac gcgctagctt ttccaaggca
    25441 ttggctaaat gatggattcc accttctaca taccaaaggc cttgctcttg ttgcatatgg
    25501 aataacatag ataatacagc tggcgcatcg tatgatgaag aacctacata tttgataaaa
    25561 tagcctaaca tttgtcgcaa gtatggattc gagatacgct tgtctatggc ctgttgcata
    25621 gtatgtacat aatcataatt aataagagca tttaatggcc catgaaattt aataatttga
    25681 gatagtgtat ctaaaccata gttgaaatac cctttttcag taatacgatc gattcgtctt
    25741 gtataattca aataattttg cagttcctct atatcctgtt tcgacaatat cgcattatgc
    25801 tgacctgttt ctttaatacc ttcatacaaa tcgatagtcg ttccatctgg aaaaaagcta
    25861 cgccattgat gtggcaatcg cttgattgta acgtagtctg acatttgctt cttgctatat
    25921 tcgaataatt tttcaaaaat ataaggcatc gttaaaatag atggacctaa atcaaagcca
    25981 aagccatctg attcatgacg attcactttg cctcctatat gattattttg ttcatataat
    26041 gagaccgaat agccactttg tgccattcga attgctgcag aaatcccacc taagccacca
    26101 ccaataacga tgatatgttt agtcatgacg ttcacctctt cgttttaata tgcgtagtaa
    26161 ctgcgttaat ctcggtcgat tatagcgttg cacaataatg attggaacat tagctatcat
    26221 tgcatagaaa atatttatat actttactaa acgagggcct ttattgaata tgatgactgg
    26281 aagtatcgat atccaatgaa tcaactctgc cctctttgtc tctataatca ttttttcaat
    26341 ttcatcagta ttgattgtca ttaaatgacg ctgatcatat atattttgat taagctgatg
    26401 accatctaaa atcttatgtt tccattttct tacgtaaaac catttgttcc aaaattttcc
    26461 atgcttctca aagttaaatg atttaaatat tatgtacttc tgacgaaaat acttatcagg
    26521 aattcttgta cctaaatttg ctatatttag ttgaacaatt aaccaataca tactacaaaa
    26581 aaatgctgtt ttaatatatt ttttcatggt tttcatctaa attgaatcac tctcaatcat
    26641 actgacatag tataaattat cactttatta cttagttccc taaagatcag tgaataaacc
    26701 gctctcattt atatttatat aaaacaaaaa cgccaatact ctcatgttag agaatattga
    26761 cgtcatttga tttacatttg attaatgaat gaaattatat gaacctgctt ggtttgctga
    26821 aattgtacga gacgtaacca caccagcacc atgtccatag ttcatttctg aaacacgaac
    26881 agaaccgttg ctgttaacgc cttcaacgta agcaacatga ccgtaatagc cttgtgttgt
    26941 ttgcatgata gcaccaactt ttggtgtatt gttcactgta tagccagatg atgcagctgc
    27001 gttagcccaa ttacttgcgt taccccatgt tgaaccaatt ttcccaccaa cacgatcaaa
    27061 tacataataa gtacattgtc ctgaagtata taagttactt cctgatgcat aaccattaga
    27121 aattgaacga ccatttgaag atggcgctgc agttgtagtc acatgaacat tattacttgt
    27181 tgtgctataa cttgcacctg aaccaccagt ataatagtta tttgttgctg tttgactatt
    27241 gtttgtatag ttattgtatt gatatgaatg attatagtag ttattgtaag atgcattgtt
    27301 ataactataa gtattgtagt tgttgtagta gtatgtgttg ttttgcgtta attgacttgg
    27361 attccaattt cctgtccaag tgtaatgata attaccttgt gcatcaattg tatacgtata
    27421 gctgtatgat tgagcgtcat tagaattata tccattgtta ttttgttctg cggcttgtgc
    27481 atcatgtcct gcaaatgcga tagtggcaag tcctgctgta gcgattgtag ctgtaacgat
    27541 ttttttcata attaaatatc ctcccaataa tcaaaacact ctatacatat agtcattagc
    27601 gactcaatta ttttaaattt tatatttttt atttttttag tatgcaaaca attataactt
    27661 ttatagtttt gcgacaaaac aaactataac agagattagg tgtcttgcgg gctttgttat
    27721 cataatgtaa tctgttccta ataaagcttc attgaatgta atataccaat tagaatgttt
    27781 acaacacttt ttctgcaccg ttactatggg atttgaaaat aaaaaagcac taaactttta
    27841 atgaaataca tcatttttga taataaatat cagaagtaat gtaatatttc aaaagtttag
    27901 tgcatcaaat tatttcttat ttgtagcatg tgtttccatc gtttttacaa tcaaatcagt
    27961 cagcgcttta ctacctgcat attctaagtg aataccgtca tatgcaaagt attccggatg
    28021 acctgcagaa gctttatacc aatcgactag atgtacatta gagcgctttt cagctgcctc
    28081 ataaattaat ttatttattc taccttcata atctctaggt actctaatag aaactaaata
    28141 tatgtctgct tttccaaaac tatccaatag ttcatttaat tgatctttcg taaatgcccc
    28201 atttgtacca agctctacta caactttttg acctttttta gcatagtctt tatattgcga
    28261 tttcacaatt ggtgtagcat caacgagttg ccgtccaact ttaccatcaa tttgtgcatt
    28321 tggtattttc ttagtaaaga cattaccaat atccaccatg accgagtcac caattaatag
    28381 tggtgatgac tttttaatat cctctttgct attcgctgtc tgcttatcct cttgcttatc
    28441 ttttttaacg acttttttct tcgttgtttt aaattccgtc ttcttctctt tcttctcggc
    28501 ttcatgttgt ttgccaagtg catcaaactg tccactgagc acaacgatag acggaaccaa
    28561 taataggata actaacactg ttctagcaaa ttgccccttc tttttaggta aaaatgcaaa
    28621 agctttaaat ccttttttac gtataggtgt ttcaataaag cgatacgaaa tttcagccat
    28681 taacgctgtt aacaaaattt ctataatata aacgtatacc ggtatttgtc cttgtacgta
    28741 ataactgttc acaaaaacaa tgataggata atgccataag tataagctat atgatcgttt
    28801 acctataatt agtaaaggtt tcatacttaa aaatttagca aataaactag aaggatgtac
    28861 cgcaattgca ataatgaata aagttgcaaa tgatataatg taaaatcctc cattatagat
    28921 ccattgatct tggtctccaa ctataaagaa caaagtcatt agaaccgcaa aaccagatat
    28981 ccctataata tctaatgata cgacaatctt tttagaaata tcttttttca aagcaaacgg
    29041 aggccaaata aatgctaata tacaaccaag caataaagtt tgcagtcgtg tatctgtccc
    29101 aaaatacaca cgtgaattat ctccagtgat gaaatgaatc actatcataa gtcctaaaga
    29161 aatcaacgat acaataaata gcgtttgaat aatatttctc ggtttaaatc tatgtaataa
    29221 gaacgtgata accaatggga aaagcaagta aaattgttct tcaatggcta aagaccataa
    29281 atgtttgagt ggttcaatag caaattggtt aaaataatct acattctgtg aaatgtacca
    29341 ccagtttgaa acatagaata tagctgcaat agcatctcgt ttcatttgta taatcaattc
    29401 cggtttaaat atcagtgtga acgtaagcac gacacagatt aaaaataaca ctgccggaat
    29461 gagtcgtttc aatcgtcgct tccaaaactc tagcaaatcg attttttgcg tccgataata
    29521 ctcacttatc aacaaacttg ttattaaata acctgaaata acgaagaatg tatctactcc
    29581 taaaaagccc ccacttaacc attgtgcatt caagtgataa ataatgattc ctataactgc
    29641 gaatgccctc aatccatcta atccaggtaa gtatcgcggg gaatacattt tttctaaacg
    29701 tttaaagtct tttgtatcca tgttaataaa cgccccattt atttttctct attttgtagc
    29761 atatcacaat atttttgaaa ataaaatatt gcactgattt tcattaattg atttaaccct
    29821 taat
//
LOCUS       DAXWFK010000013        26939 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.013, whole genome shotgun sequence.
ACCESSION   DAXWFK010000013 DAXWFK010000000
VERSION     DAXWFK010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 26939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26939
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.013"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(74..1192)
                     /locus_tag="ACGEXS_000470"
     CDS             complement(74..1192)
                     /locus_tag="ACGEXS_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFF8011517.1"
                     /translation="MNKQQSKVRYSIRKVSIGILSISIGMFLALGMSNKAYADEIDKS
                     KDFTRGYEQNVFAKSELNANKNTTKDKIKNEGAVKTSDTSLKLDNKSAISNGNEINQD
                     IKISNTPKNSSQGNNLVINNNELTKEIKIANLEAQNSNQKKTNKVTNNYFGYYSFREA
                     PKTQIYTVKKGDTLSAIALKYKTTVSNIQNTNNIANPNLIFIGQKLKVPMTPLVEPKP
                     KTVSSNNKSNSNSSTLNYLKTLENRGWDFDGSYGWQCFDLVNVYWNHLYGHGLKGYGA
                     KDIPYANNFNSEAKIYHNTPTFKAEPGDLVVFSGRFGGGYGHTAIVLNGDYDGKLMKF
                     QSLDQNWNNGGWRKAEVAHKVVHNYENDMIFIRPFKKA"
     gene            complement(1419..2327)
                     /gene="sucD"
                     /locus_tag="ACGEXS_000471"
     CDS             complement(1419..2327)
                     /gene="sucD"
                     /locus_tag="ACGEXS_000471"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFF8011518.1"
                     /translation="MSVFIDKNTKVMVQGITGSTALFHTKQMLDYGTKIVAGVTPGKG
                     GQVVEGVPVFNTVEEAKNETGATVSVIYVPAPFAADSILEAADADLDMVICITEHIPV
                     LDMVKVKRYLQGRKTRLVGPNCPGVITADECKIGIMPGYIHKKGHVGVVSRSGTLTYE
                     AVHQLTEEGIGQTTAVGIGGDPVNGTNFIDVLKAFNEDDETKAVVMIGEIGGTAEEEA
                     AEWIKANMTKPVVGFIGGQTAPPGKRMGHAGAIISGGKGTAEEKIKTLNSCGVKTAAT
                     PSEIGSTLIEAAKEAGIYESLLTVNK"
     gene            complement(2349..3515)
                     /gene="sucC"
                     /locus_tag="ACGEXS_000472"
     CDS             complement(2349..3515)
                     /gene="sucC"
                     /locus_tag="ACGEXS_000472"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFF8011519.1"
                     /translation="MNIHEYQGKEIFRSMGVAVPEGRVAFTAEEAVEKAKELNSDVYV
                     VKAQIHAGGRGKAGGVKIAKSLSEVETYAKELLGKTLVTHQTGPEGKEIKRLYIEEGC
                     AIQKEYYVGFVIDRATDQVTLMASEEGGTEIEEVAAKTPEKIFKETIDPVIGLSPFQA
                     RRIAFNINIPKESVNKAAKFLLALYNVFIEKDCSIVEINPLVTTADGDVLALDAKINF
                     DDNALFRHKDVVELRDLEEEDPKEIEASKHDLSYIALDGDIGCMVNGAGLAMATMDTI
                     NHFGGNPANFLDAGGSATREKVTEAFKIILGDENVKGIFVNIFGGIMKCDVIAEGIVE
                     AVKEVDLTLPLVVRLEGTNVELGKKILKDSGLAIEPAATMAEGAQKIVKLVKEA"
     gene            complement(3624..4391)
                     /locus_tag="ACGEXS_000473"
     CDS             complement(3624..4391)
                     /locus_tag="ACGEXS_000473"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFF8011520.1"
                     /translation="MTLTIKEVTQLINAVNTIEELENHECFLDERKGVQNAIARRRKA
                     LEKEQALKEKYVEMTYFENEILKEHPNAIICGIDEVGRGPLAGPVVACATILNSNHNY
                     LGLDDSKKVPVTKRLELNEALKNEVTAFAYGIATAEEIDEFNIYKATQIAMQRAIDGL
                     SVQPTHLLIDAMTLDNALPQVSLIKGDARSVSIAAASIMAKVFRDDYMTQLSKDYPEY
                     GFEKNAGYGTKQHLLAIDDIGIMKEHRKSFEPIKSLL"
     gene            complement(4375..5259)
                     /gene="ylqF"
                     /locus_tag="ACGEXS_000474"
     CDS             complement(4375..5259)
                     /gene="ylqF"
                     /locus_tag="ACGEXS_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFF8011521.1"
                     /translation="MVIQWYPGHMAKAKREVSEQLKKVDVVFELVDARIPYSSRNPMI
                     DEVINQKPRVVILNKKDMSNLNEMSKWEQFFIDKGYYPVSVDAKHGKNLKKVEAAAIK
                     ATAEKFEREKAKGLKPRAIRAMIVGIPNVGKSTLINKLAKRSIAQTGNKPGVTKQQQW
                     IKVGNALQLLDTPGIIWPKFEDEEVGKKLSLTGAIKDSIVHLDEVAIYGLNFLIQNDL
                     ARLKSHYNIEVPEDAEIIAWFDAIGKKRGLIRRGNEIDYEAVIELIIYDIRNAKIGNY
                     CFDIFKDMTEELANDANN"
     gene            5660..8266
                     /gene="yfhO"
                     /locus_tag="ACGEXS_000475"
     CDS             5660..8266
                     /gene="yfhO"
                     /locus_tag="ACGEXS_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid-specific glycosyltransferase
                     YfhO"
                     /protein_id="HFF8011522.1"
                     /translation="MLKKWLNSNVKQFFVITFISVILTLILFSTHIYDYIVNGTVFSG
                     AGDGFRQMMPFQMYLYEHLRSFSSLYDASFGLGGDYMKGLSYYYSLSPLMWLNFLFIK
                     IGETVGIFNPTTIHFWPTNQLIMAMIRAIITFVVTFYLFKILHFKRSANMIATILYGM
                     STVVIYFNFTWSFYGNLLYLLPLSILGLERYFQQRKIGIFIVAIALTLFSNFYFSYYQ
                     AIIIGCYYLYRLIFTYKYDIVSRTQKLICVISATVLSVLSSVFGLFTGISAFLENDRK
                     QNPNVDIPFLTPLDYHYFFFSDGFYITISILTIVALLSFKLYRFYFYRLFAIVTWILF
                     IGSLSQYFDSAFNGFSFPERRWVYILALSSSALCGLFIQHLSTLNMKYYLIRTIPVCI
                     IAILYVLLSPTHPLALIVGIILLIVLAVILKFSLWRYKKLTVAILVLIVMIQQIVILD
                     NNKNMAIKPYQQSLSTLKQHDYHSNYVNQLIKKINQNATGSFNRIDYMSDYALNSPFI
                     YHYNGISLYSSIFNGDILKYYDKTLQINMPIDKNSTYRLLGNRQNLLSLWNVNDRIRV
                     NHDDNLPYGFKIKSEHKDNKVRWIHSKNTIHYPSAHITNKVFSNKELKSPLDKEQAML
                     QGIVSNNIKDVNTHFKANKNLLSDSTIKLNSAAWQSPTKHLLQVKQNNGGLTVQLPKS
                     VSNQFKDLYFEMDLELLSPDKAHDVKVNEYTQERNKLTYKYRRVVTPVTIRIKAPDRI
                     RLSLPKGKYRVNLKGIYGEDYTTLKDASNSLEAVKVSKTKHGYTITKNKNSSGYIVLP
                     TAYNQGMKATSGDQSLKVEQVNGVMTGIKAPKNITKIQLSYTPPYYYLLITITIFGII
                     CSIIFTRWARQK"
     gene            complement(8510..8860)
                     /gene="rplS"
                     /locus_tag="ACGEXS_000476"
     CDS             complement(8510..8860)
                     /gene="rplS"
                     /locus_tag="ACGEXS_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011303276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFF8011523.1"
                     /translation="MTNHKLIEAVTKSQLRTDLPSFRPGDTLRVHVRIIEGTRERIQV
                     FEGVVIKRRGGGVSETFTVRKISSGVGVERTFPLHTPKIEKIEVKRRGKVRRAKLYYL
                     RSLRGKAARIQEIR"
     gene            complement(8963..9700)
                     /gene="trmD"
                     /locus_tag="ACGEXS_000477"
     CDS             complement(8963..9700)
                     /gene="trmD"
                     /locus_tag="ACGEXS_000477"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFF8011524.1"
                     /translation="MKIDYLTLFPEMFDGVLNHSIMKRAQENNKLQINTVNFRDYAIN
                     KHNQVDDYPYGGGQGMVLKPEPVFNAMEDLDVTEQTRVILMCPQGEPFSHQKAVELSK
                     ADHIVFICGHYEGYDERIRTHLVTDEISMGDYVLTGGELPAMTMTDAIVRLIPGVLGN
                     EQSHQDDSFSDGLLEFPQYTRPREFKGLTVPDVLLSGNHANIDAWRHEQKLIRTYNKR
                     PDLIEKYPLTNADKQILERYKIGLKKG"
     gene            complement(9700..10203)
                     /gene="rimM"
                     /locus_tag="ACGEXS_000478"
     CDS             complement(9700..10203)
                     /gene="rimM"
                     /locus_tag="ACGEXS_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499746.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFF8011525.1"
                     /translation="MRVEVGQIVNTHGIKGEIKVKSNSDFTDVRFQPGQVLTVVHNNN
                     DLEYTVKSHRVHKGLHMLTFEGINNINDIEHLKGSSIYQERDHEDIVLEENEFYYSDI
                     IGCTVFDDQETPIGRVINIFETGANDVWVIKGSKEYLIPYIADVVKEVDVENKKIIIT
                     PMEGLLD"
     gene            complement(10391..10666)
                     /gene="rpsP"
                     /locus_tag="ACGEXS_000479"
     CDS             complement(10391..10666)
                     /gene="rpsP"
                     /locus_tag="ACGEXS_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFF8011526.1"
                     /translation="MAVKIRLTRLGSKRNPFYRIVVADARSPRDGRIIEQIGTYNPTS
                     ANAPEIKVDEALALKWLNDGAKPTDTVHNILSKEGIMKKFDEQKKAK"
     gene            complement(11101..12468)
                     /gene="ffh"
                     /locus_tag="ACGEXS_000480"
     CDS             complement(11101..12468)
                     /gene="ffh"
                     /locus_tag="ACGEXS_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002457378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFF8011527.1"
                     /translation="MAFEGLSERLQATMQKMRGKGKLTEADIKIMMREVRLALLEADV
                     NFKVVKEFIKTVSERALGSDVMQSLTPGQQVIKIVQDELTQLMGGENTSINMSNKPPT
                     VVMMVGLQGAGKTTTAGKLALLMRKKYNKKPMLVAADIYRPAAINQLQTVGKQIDIPV
                     YSEGDQVKPQQIVTNALKHAKEEHLDFVIIDTAGRLHIDEALMNELKEVKDIAKPNEI
                     MLVVDSMTGQDAVNVAESFDDQLDVTGVTLTKLDGDTRGGAALSIRSVTQKPIKFVGM
                     SEKLDGLELFHPERMASRILGMGDVLSLIEKAQQDVDQEKAKDLEKKMRESSFTLDDF
                     LEQLDQVKNLGPLDDIMKMIPGMNKMKGLDKLNMSEKQIDHIKAIIQSMTPAERNNPD
                     TLNVSRKKRIAKGSGRSLQEVNRLMKQFNDMKKMMKQFTGGGKGKKGKRNQMQNMLKG
                     MNLPF"
     gene            complement(12494..12826)
                     /locus_tag="ACGEXS_000481"
     CDS             complement(12494..12826)
                     /locus_tag="ACGEXS_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFF8011528.1"
                     /translation="MGQNDLVKTLRMNYLFDFYQSLLTNKQRNYLELFYLEDYSLSEI
                     ADTFNVSRQAVYDNIRRTGDLVEDYEKKLELYQKFEQRREIYDEMKQHLSNPEQIQRY
                     IQQLEDLE"
     gene            complement(12813..14063)
                     /gene="ftsY"
                     /locus_tag="ACGEXS_000482"
     CDS             complement(12813..14063)
                     /gene="ftsY"
                     /locus_tag="ACGEXS_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFF8011529.1"
                     /translation="MSFFKRLKDKFATNKENEEVKSLTEEQGQDKLEDTHSEGSTQDA
                     NDLAENAEVKKKPRKLSEADFDDDGLISIEDFEEIEAQKMGAKFKAGLEKSRQNFQEQ
                     LNNLIARYRKVDEDFFEALEEMLITADVGFNTVMTLTEELRMEAQRRNIQDTEDLREV
                     IVEKIVEIYHQEDDNSEAMNLEDGRLNVILMVGVNGVGKTTTIGKLAYRYKMEGKKVM
                     LAAGDTFRAGAIDQLKVWGERVGVDVISQSEGSDPAAVMYDAINAAKNKGVDILICDT
                     AGRLQNKTNLMQELEKVKRVINRAVPDAPHEALLCLDATTGQNALSQARNFKEVTNVT
                     GIVLTKLDGTAKGGIVLAIRNELHIPVKYVGLGEQLDDLQPFNPESYVYGLFADMIEQ
                     NEEITTVENDQIVTEEKDDNHGSK"
     gene            complement(14063..17629)
                     /gene="smc"
                     /locus_tag="ACGEXS_000483"
     CDS             complement(14063..17629)
                     /gene="smc"
                     /locus_tag="ACGEXS_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFF8011530.1"
                     /translation="MVYLKSIDAIGFKSFADQTNVQFDKGVTAIVGPNGSGKSNITDA
                     IKWVLGEQSAKSLRGSKMEDIIFSGAEHRKAQNYAEVQLRLDNHSKKLSVDENEVIVT
                     RRLYRSGESEYYINNDRARLKDIADLFLDSGLGKEAYSIISQGRVDEILNAKPIDRRQ
                     IIEESAGVLKYKKRKAESLNKLDQTEDNLTRVEDILYDLEGRVEPLKEEAAIAKEYKT
                     LSHQMKHSDIVVTVHDIDQYTNDNRQLDQRLNDLQGQQANKEADKQRLSQQIQQYKGK
                     RHQLDNDVESLNYQLVKATEAFEKYTGQLNVLEERKKNQSETNARYEEEQENLMELLE
                     NISNEISEAQDTYKSLKSKQKELNAVIRELEEQLYVSDEAHDEKLEEIKNEYYTLMSE
                     QSDVNNDIRFLKHTIEENEAKKSRLDSRLVEVFEQLKDIQGQIKTTKKEYQQTNKELS
                     AVDKEIKNIEKDLTDTKKAQNEYEEKLYQAYRYTEKMKTRIDSLATQEEEYTYFFNGV
                     KHILKAKNKELKGIYGAVAEIIDVPSKLTQAIETALGASLQHVIVDSEKDGRQAIQFL
                     KERNLGRATFLPLNVIQSRVVATDIKSIAKEANGFISIASEAVKVAPEYQNIIGNLLG
                     NTIIVDHLKHANELARAIKYRTRIVTLEGDIVNPGGSMTGGGARKSKSILSQKDELTT
                     MRHQLEDYLRQTESFEQQFKELKIKSDQLSELYFEKSQKHNTLKEQVHHFEMELDRLT
                     TQETQIKNDHEEFEFEKNDGYTSDKSRQTLSEKETYLESIKASLKRLEDEIERYTKLS
                     KEGKESVTKTQQTLHQKQSDLAVVKERIKTQQQTIDRLNNQNQQTKHQLKDVKEKIAF
                     FNSDEVMGEQAFQNIKDQINGQQETRTRLSDELDKLKQQRIELNEQIDAQEAKLQVCH
                     QDILAIENHYQDIKAEQSKLDVLIHHAIDHLNDEYQLTVERAKSEYTSDESIDALRKK
                     VKLMKMSIDELGPVNLNAIEQFEELNERYTFLSEQRTDLRKAKETLEQIISEMDQEVT
                     ERFKETFHAIQGHFTAVFKQLFGGGDAELQLTEADYLTAGIDIVVQPPGKKLQHLSLL
                     SGGERALTAIALLFAILKVRSAPFVILDEVEAALDEANVIRYAKYLNELSDETQFIVI
                     THRKGTMEFADRLYGVTMQESGVTKLVSVNLNTIDDVLKEEQ"
     gene            complement(17776..18507)
                     /gene="rnc"
                     /locus_tag="ACGEXS_000484"
     CDS             complement(17776..18507)
                     /gene="rnc"
                     /locus_tag="ACGEXS_000484"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFF8011531.1"
                     /translation="MSKQKKSEIVNRFRKRFDTKMTELGFTYQNIDLYQQAFSHSSFI
                     NDFNMNRLDHNERLEFLGDAVLELTVSRYLFDKHPNLPEGNLTKMRATIVCEPSLVIF
                     ANKIGLNEMILLGKGEEKTGGRTRPSLISDAFEAFIGALYLDQGLDIVWKFAEKVIFP
                     HVEQNELLGVVDFKTQFQEYVHQQNKGDVTYNLIKEEGPAHHRLFTSEVILQGEAIAE
                     GKGKTKKESEQRAAESAYKQLKQIK"
     gene            complement(18623..18856)
                     /locus_tag="ACGEXS_000485"
     CDS             complement(18623..18856)
                     /locus_tag="ACGEXS_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002510960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFF8011532.1"
                     /translation="MENFDKVKDIIVDRLGVDADKVTEDASFKDDLGADSLDIAELVM
                     ELEDEFGTEIPDEEAEKINTVGDAVKFINSLEK"
     gene            complement(19099..19833)
                     /gene="fabG"
                     /locus_tag="ACGEXS_000486"
     CDS             complement(19099..19833)
                     /gene="fabG"
                     /locus_tag="ACGEXS_000486"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFF8011533.1"
                     /translation="MTKSALVTGASRGIGRSIALQLAEEGYNVAVNYAGSKEKAEAVV
                     EEIKAKGVDSFAIQANVADADEVKAMIKEVVSQFGSLDVLVNNAGITRDNLLMRMKEQ
                     EWDDVIDTNLKGVFNCIQKATPQMLRQRSGAIINLSSVVGAVGNPGQANYVATKAGVI
                     GLTKSAARELASRGITVNAVAPGFIVSDMTDALSDELKEQMLTQIPLARFGQDTDIAN
                     TVAFLASDKAKYITGQTIHVNGGMYM"
     gene            complement(19826..20752)
                     /gene="fabD"
                     /locus_tag="ACGEXS_000487"
     CDS             complement(19826..20752)
                     /gene="fabD"
                     /locus_tag="ACGEXS_000487"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFF8011534.1"
                     /translation="MSKTAIIFPGQGAQKVGVAQDLFNNNDQATEILTSAANTLDFDI
                     LETMFTDEEGKLGETENTQPALLTHSSALLAALKNLNPDFTMGHSLGEYSSLVAADVL
                     SFEDAVKIVRKRGQLMAQAFPTGVGSMAAVLGLDFDKVDEICKSLSSDDKIIEPANIN
                     CPGQIVVSGHKALIDELVEKGKSLGAKRVMPLAVSGPFHSSLMKVIEEDFSSYINQFE
                     WRDAKFPVVQNVNAQGETDKEVIKSNMVKQLYSPVQFINSTEWLIDQGVDHFIEIGPG
                     KVLSGLIKKINRDVKLTSIQTLEDVKGWNEND"
     gene            complement(20745..21731)
                     /gene="plsX"
                     /locus_tag="ACGEXS_000488"
     CDS             complement(20745..21731)
                     /gene="plsX"
                     /locus_tag="ACGEXS_000488"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFF8011535.1"
                     /translation="MVKLAIDMMGGDNAPDIVLEAVQKAVEDFKDLEIILFGDEKKYN
                     LNHERIEFRHCSEKIEMEDEPVRAIKRKKDSSMVKMAEAVKSGEADGCVSAGNTGALM
                     SAGLFIVGRIKGVARPALVVTLPTIDGKGFVFLDVGANADAKPEHLLQYAQLGDIYAQ
                     KIRGIDNPKISLLNIGTEPAKGNSLTKKSYELLNHDHSLNFVGNIEAKTLMDGDTDVV
                     VTDGYTGNMVLKNLEGTAKSIGKMLKDTIMSSTKNKLAGAILKKDLAEFAKKMDYSEY
                     GGSVLLGLEGTVVKAHGSSNAKAFYSAIRQAKIAGEQNIVQTMKETVGESNE"
     gene            complement(21736..22308)
                     /gene="fapR"
                     /locus_tag="ACGEXS_000489"
     CDS             complement(21736..22308)
                     /gene="fapR"
                     /locus_tag="ACGEXS_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045717 - negative regulation of fatty acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HFF8011536.1"
                     /translation="MRGETLKLKKDKRREAIRQQIDSNPFITDHELSDLFQVSIQTIR
                     LDRTYLNIPELRKRIKLVAEKNYDQISSIEEQEFIGDLIQVNPNVKAQSILDITSDSV
                     FHKTGIARGHVLFAQANSLCVALIKQPTVLTHESSIQFIEKVKLNDTVRAEARVVNQT
                     AKHYYVEVKSYVKHTLVFKGNFKMFYDKRG"
     gene            complement(22511..24571)
                     /gene="recG"
                     /locus_tag="ACGEXS_000490"
     CDS             complement(22511..24571)
                     /gene="recG"
                     /locus_tag="ACGEXS_000490"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFF8011537.1"
                     /translation="MAKVNLIESPYSLLQLKGIGPKKIEVLQQLNIHTVEDLVLYLPT
                     RYEDNTVIDLNQAEDQSNVTIEGQVYTAPVVAFFGRNKSKLTVHLMVNNIAVKCIFFN
                     QPYLKKKIELNQTITVKGKWNRVKQEITGNRVFFNSQGTQTQENADVQLEPVYRIKEG
                     IKQKQIRDQIRQALNDVTIHEWLTDELREKYKLETLDFTLNTLHHPKSKEDLLRARRT
                     YAFTELFLFELRMQWLNRLEKSSDEAIEVDYDIDQVKSFIDRLPFELTEAQKSSVNEI
                     FRDLKAPIRMHRLLQGDVGSGKTVVAAICMYALKTAGYQSALMVPTEILAEQHAESLM
                     ALFGDSMNVALLTGSVKGKKRKILLEQLENGTIDCLIGTHALIQDDVIFHNVGLVITD
                     EQHRFGVNQRQLLREKGAMTNVLFMTATPIPRTLAISVFGEMDVSSIKQLPKGRKPII
                     TTWAKHEQYDKVLMQMTSELKKGRQAYVICPLIESSEHLEDVQNVVALYESLQQYYGV
                     SRVGLLHGKLSADEKDEVMQKFSNHEINVLVSTTVVEVGVNVPNATFMMIYDADRFGL
                     STLHQLRGRVGRSDQQSYCVLIASPKTETGIERMTIMTQTTDGFELSERDLEMRGPGD
                     FFGVKQSGLPDFLVANLVEDYRMLEVARDEAAELIQSGVFFENTYQHLRHFVEENLLH
                     RSFD"
     gene            complement(24761..26407)
                     /gene="fakA"
                     /locus_tag="ACGEXS_000491"
     CDS             complement(24761..26407)
                     /gene="fakA"
                     /locus_tag="ACGEXS_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase catalytic subunit FakA"
                     /protein_id="HFF8011538.1"
                     /translation="MISKINGKLFADMIIQGAQNLSNNADLVDSLNVYPVPDGDTGTN
                     MNLTMTSGREEVENNLSKNIGELGKTFSKGLLMGARGNSGVILSQLFRGFCKNIESES
                     EINSKLLAESFQAGVETAYKAVMKPVEGTILTVAKDAAQAAIEKANNTEDCIELMEYI
                     IVKANESLENTPNLLAVLKEVGVVDSGGKGLLCVYEGFLKALKGEKVEAKVAKIDKDE
                     FVHDEHDFHGVINTEDIIYGYCTEMMVRFGKNKKAFDEQEFRQDMSQFGDSLLVINDE
                     EIVKVHVHTEYPGKVFNYGQQYGELIKLKVENMREQHREVIRKEQHTAKPKMETVETA
                     IITISMGEGISEIFKSMGATHIISGGQTMNPSTEDIVKVIEQSKCKRAIILPNNKNIL
                     MASEQAASIVDAEAVVIPTKSIPQGISALFQYDVDATLEENKAQMADSVNNVKSGSLT
                     YAVRDTKIDGVEIKKDAFMGLIEDKIVSSQSDQLTTVTELLNEMLAEDSEILTVIIGQ
                     DAEQAVTDNMINWIEEQYPDVEVEVHEGGQPIYQYFFSVE"
     gene            complement(26422..26796)
                     /locus_tag="ACGEXS_000492"
     CDS             complement(26422..26796)
                     /locus_tag="ACGEXS_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFF8011539.1"
                     /translation="MTLEISNDYGKIDISNEVIASVVGGKAVECYGIVGMASRQQVRD
                     GIAEILGHENYAKGIKVTENNGVVDIDMYIIVSYGVKISEVANNVQSTVKYTLEKSLN
                     VSVNSINIYVQGVRVNNTGKKA"
BASE COUNT         8047 a         5262 c         3599 g        10031 t
ORIGIN      
        1 tggtgtagtt ggtaaattct tcttcactta aagttgaaaa tttcatattt caatgtcctg
       61 ccttcgattt aaattatgct tttttaaatg gtctaataaa aatcatatca ttttcataat
      121 tatgaacaac tttatgtgca acctctgctt tacgccatcc accattattc cagttttgat
      181 ctaaactttg gaacttcatt aattttccat cataatcacc atttaagaca atagctgtat
      241 gaccatatcc tccaccaaat cttccactaa aaaccactaa gtccccaggt tcagctttga
      301 aagttggtgt gttgtgataa attttagctt cactattaaa attatttgca tatggtatat
      361 ctttagctcc atatcctttt aatccatgac cataaagatg attccaatat acattaacta
      421 aatcgaaaca ttgccatcca taactaccgt cgaaatccca tcctctattc tctaatgttt
      481 tcaaataatt taatgtactg ctattactat tacttttatt atttgaagac actgtttttg
      541 gttttggttc tactaatggt gtcattggca cttttaattt ttgaccaata aatattaaat
      601 taggatttgc tatattattt gtattttgaa tatttgaaac tgtagttttg tattttaatg
      661 ctatagcact aagtgtgtct ccttttttta cagtatagat ttgtgttttt ggagcttctc
      721 taaaactgta gtaaccaaag taattattag taactttatt cgttttcttc tgattagaat
      781 tttgagcttc caagtttgca attttaattt ctttagtaag ttcattgtta ttaataacta
      841 gattgttacc ttggcttgag tttttcggag tatttgaaat ctttatatct tgattaattt
      901 catttccgtt tgaaattgct gatttgttgt ctaactttaa acttgtgtcc gatgttttaa
      961 cagcaccttc attttttatt ttgtcttttg tcgtattttt attagcattt aactctgatt
     1021 tcgcgaatac attttgctca taccctcttg taaaatcttt agatttatca atttcatctg
     1081 catatgcttt gttcgacata cccaatgcca aaaacatacc tattgaaatt gacaaaattc
     1141 caatactaac ttttctaatt gaatagcgta ctttactttg ttgtttattc atgatgaaac
     1201 actccttaca ataatataca aacattataa catagtgcgc tatatatttt aattaaaatt
     1261 ttctttgttt tataaagcta ttgtaggagg taaaataatt aacttgtctt tccaaaatat
     1321 gaaaagtgta ctaaaattca tcgcacgaca aatagcccat ttccgatact tttataaagt
     1381 atggaatggg ctatagccat ttatatcatc ttttaacttt atttattaac agttaataat
     1441 gattcataaa tacctgcttc tttagcagct tcaattaatg ttgaaccaat ttctgaaggt
     1501 gttgccgctg ttttcacacc acaactattt aatgttttaa ttttctcttc agcagtacct
     1561 ttaccacctg aaatgattgc accagcatgt cccatacgtt ttccaggagg tgctgtttgt
     1621 ccaccgataa agcctacaac tggttttgtc atattcgctt taatccattc agctgcttct
     1681 tcttcagccg taccaccgat ttcaccaatc ataacaactg ctttcgtttc gtcatcttca
     1741 ttgaatgctt ttaaaacatc aataaagttt gttccgttga ctgggtctcc accaatacca
     1801 acagctgtag tttgaccaat accttcttca gtcaattggt gcactgcttc atatgttaat
     1861 gtacctgaac gagatactac accaacatga ccttttttgt gaatatagcc aggcataata
     1921 ccaattttac attcatctgc tgtaatcaca cctggacagt tcggaccaac taaacgtgtt
     1981 tttctacctt gtaagtagcg tttaacttta accatgtcta atacaggaat atgttcagtg
     2041 atacaaataa ccatatctaa gtctgcatca gctgcttcta aaattgagtc tgcagcaaat
     2101 ggtgctggaa cgtaaatgac tgaaaccgtt gccccagttt catttttagc ttcttcaaca
     2161 gtgttgaaaa caggaacgcc ttcaacaact tgaccacctt taccaggcgt cacacctgct
     2221 actattttcg taccataatc aagcatttgt tttgtatgga aaagggcagt agaccctgta
     2281 ataccttgta ccattacttt agtattctta tctataaata cactcatctt agtgctccca
     2341 tcctttcctt atgcttcttt gactagttta acaatttttt gtgcaccttc agccattgtt
     2401 gctgctggtt caattgctaa tcctgagtct tttaagattt ttttacctaa ctcaacattt
     2461 gtaccttcta ggcgtacaac tagtggtaaa gttaaatcta cttcttttac agcttcaacg
     2521 ataccttctg cgataacatc acatttcata atgccaccga aaatgtttac aaaaatacct
     2581 ttaacatttt catcacctaa aatgatttta aatgcttcag ttactttttc tctagtagcg
     2641 cttccgcctg catctaagaa attggctggg tttccaccga aatgattaat cgtatccatt
     2701 gttgccatgg ctaaacctgc accattaacc atacatccga tgtcaccatc taatgcaatg
     2761 tatgataaat catgtttaga cgcttcaatc tctttcggat cttcttcttc taaatcacgt
     2821 aattctacaa catctttatg tctgaataat gcattatcat caaaattaat tttagcatct
     2881 aatgccaata catcaccatc agctgttgta actaatgggt tgatttctac gattgaacaa
     2941 tctttttcaa tgaatacatt ataaagtgct aataagaatt tagcagcttt gttaacagat
     3001 tctttaggaa tattaatatt aaacgcaatt cgtcttgctt ggaatggtga aagtccgatt
     3061 actggatcga tagtttcttt gaagatcttt tcaggagtct tcgcagcaac ttcttcaatc
     3121 tcagtgcccc cttcttcaga cgccatcaat gttacttggt cagtcgcacg atcaataacg
     3181 aatccaacgt aatattcttt ttgaatagca caaccttctt cgatatataa acgcttaatt
     3241 tctttacctt ctggaccagt ttgatgtgtc accaaagttt tccctaataa ttcttttgca
     3301 tatgtttcta cctcagataa agatttagca atttttactc cgcctgcttt acctctacct
     3361 ccagcatgaa tttgtgcttt tacaacataa acatcagaat ttaattcttt tgctttctcc
     3421 accgcttctt cagcagtaaa tgctactcgt ccttctggaa ctgcaacgcc cattgaacga
     3481 aatatttctt taccttgata ctcgtggata ttcatcttcc atcctcctgt tacttaggtt
     3541 aagttccctt acaattataa aaaatgtaag cgctattgta aacttaaatg ctactttttt
     3601 atcatttaat tgaattttac gatttacagt aacgatttta taggttcaaa gctttttcta
     3661 tgctctttca taatgccaat atcatcgatt gctagtaaat gttgtttggt accgtaaccc
     3721 gcgttttttt caaaaccata ttcaggataa tctttagata actgtgtcat ataatcatca
     3781 cgaaaaacct ttgccatgat acttgcagct gcaatggaca cacttcttgc atcacccttg
     3841 attaaagata cttgaggcag tgcattatca agcgtcatcg cgtctatcaa taaatgcgtt
     3901 ggttgtactg ataatccatc aatagctcgc tgcatggcga tttgagtagc tttataaata
     3961 ttaaattcat ctatttcttc agctgtcgcg atcccatatg caaaagcagt aacttcattt
     4021 tttagtgctt catttaattc tagacgtttc gtaacaggta cttttttcga gtcatcaagg
     4081 cccaaataat tgtgatttga atttaaaatt gttgcgcatg caacgactgg acctgctaaa
     4141 ggtcctcttc caacttcatc aatcccacaa ataatagcat taggatgctc ttttaatatt
     4201 tcattttcaa agtaagtcat ttcaacatac ttttctttta aagcttgttc tttttctaac
     4261 gcttttctgc gcctagctat ggcattttga acaccttttc gctcatctaa aaagcattca
     4321 tgattttcta attcttctat tgtattaacc gcattaatca actgcgtaac ttctttaatt
     4381 gttagcgtca tttgctaatt cctcagtcat atctttaaaa atatcaaaac aataatttcc
     4441 tattttagca tttcgaatat cataaataat cagttcaatg actgcttcgt aatcaatttc
     4501 attaccacgt cgaattaagc cacgtttttt ccctatcgca tcaaaccacg ctatgatttc
     4561 tgcatcttca ggaacttcaa tattataatg tgactttaat cgcgctaaat cattttgaat
     4621 taaaaagttt aatccataga tggcaacttc atctaagtgc acaatactat cttttatcgc
     4681 accagttaaa ctcaacttct taccgacttc ttcatcttca aatttaggcc aaattatccc
     4741 tggtgtgtct aatagttgta atgcattacc aactttaatc cattgttgtt gtttggtcac
     4801 acctggttta ttaccagtct gcgcaatact acgctttgcc agtttattta ttaatgtgga
     4861 tttaccaaca tttggaattc caacgatcat tgctcttatc gctctaggtt taagtccttt
     4921 cgctttttcg cgttcaaatt tttcagcagt cgccttaatt gctgcagctt ccactttctt
     4981 taaattttta ccgtgcttag catccactga tacaggatag tatcctttat caataaaaaa
     5041 ttgttcccat tttgacatct catttaaatt agacatatct tttttattta atataacaac
     5101 acgtggtttt tggttaataa cttcatctat catagggttt cttgaactat atggaattct
     5161 tgcatctact agttcaaaca ctacatctac tttttttaat tgttcactta cttctctttt
     5221 ggctttcgcc atatgtcctg gataccattg aataaccatt caattcacct tactttcgtc
     5281 aattcttttc tcattattaa aatctataac ctgttaatta tagaacaaat tcattaattc
     5341 aactatattt attttaacat tgtcacactt cttaacaatg ctttagattt aggtataagt
     5401 gattatttca agttttctac aattaaaaat catcatttgt taaatctaag ttacttatta
     5461 agtgacattc atctttatca gtatgacatt tttattattc aatatatttg tttaatgata
     5521 aactgaaaat gatccaaagt ttaagtaact atgataaggt atctgttcca cagtcatctt
     5581 cgtctttacc tatcaagaac ctcattaact ttagtttcag ttaaacttaa ttttaaattt
     5641 gaaagttgag gataatgttg tgttaaaaaa gtggctaaat tcaaacgtca aacaattctt
     5701 tgttataact ttcattagtg taatattaac gcttatttta ttttctactc atatctatga
     5761 ttatattgtg aatggtactg tttttagcgg ggctggagat ggattccgtc aaatgatgcc
     5821 atttcaaatg tatttgtatg aacatctacg tagtttttct agtttatatg atgcatcgtt
     5881 tggattaggt ggcgattata tgaaaggact atcatattat tattcgctgt cacctttaat
     5941 gtggctaaat tttctattca ttaaaatagg agaaacggtt ggtatattta atccgacgac
     6001 aatacatttt tggccgacaa accaacttat tatggctatg atacgagcta tcataacatt
     6061 tgtcgtgacc ttctacttat ttaaaatatt acactttaaa cgctcagcaa atatgatcgc
     6121 tacgatttta tacggcatgt caactgtcgt tatatacttt aattttactt ggtcatttta
     6181 tggaaattta ttatatttat tgccattatc gattcttggt ttggaaagat attttcaaca
     6241 acgcaaaatc ggtattttca ttgttgcgat agccttaaca ctatttagca atttttattt
     6301 cagttattat caagctatta ttataggttg ctactattta tatcgactca ttttcactta
     6361 caaatatgac attgtctcta gaacacaaaa attaatttgc gtcatatctg ctacagtttt
     6421 gagtgtgtta tcaagtgtat ttggtttatt cactggcatt tctgcgtttt tggaaaatga
     6481 cagaaagcaa aatcccaatg ttgatatacc gtttttgaca ccacttgatt atcattattt
     6541 tttctttagc gatggatttt atattacgat ttcaattctt actatcgttg cattattgtc
     6601 attcaaactg tatcgttttt acttttatag acttttcgca atagtaacat ggatattatt
     6661 tataggttca ttatcacagt atttcgacag tgcttttaat ggtttttcat ttccagaaag
     6721 gcgttgggtg tatatcttag cactatcatc aagtgctctt tgcggattgt ttattcaaca
     6781 tttatcaaca ttaaatatga aatattattt aatcagaaca atacccgtat gcatcatcgc
     6841 aatactttat gtattactat caccgacaca cccacttgca cttatagtag gtattatcct
     6901 gctaatagtg cttgccgtta ttttaaaatt tagtttatgg cgttataaaa aattaaccgt
     6961 tgcaatatta gtattaatcg ttatgattca acaaatcgtc attttagata acaacaaaaa
     7021 catggcaatc aaaccttatc aacaatcatt atcaacgttg aaacaacatg attaccatag
     7081 taactatgta aaccagctta taaaaaagat aaatcaaaat gcaacaggct catttaatcg
     7141 cattgattat atgtcagact atgcattaaa ttcaccattt atatatcatt ataatggcat
     7201 ttcattatat tctagtattt ttaatggaga cattttaaaa tattatgaca agacactcca
     7261 aattaatatg ccaatcgata aaaacagcac ttatagatta cttggcaatc gtcaaaattt
     7321 actatcactt tggaatgtta atgatcgaat tagagtgaat catgatgaca acttaccata
     7381 tggatttaaa attaagtctg aacacaaaga caataaagtt cgttggattc attctaaaaa
     7441 taccatccat tatccaagtg cacatattac aaataaggtc ttttccaata aagaattaaa
     7501 atctccatta gataaagaac aagcaatgtt gcaagggatt gtttctaaca atattaaaga
     7561 tgttaataca cattttaaag ccaataaaaa tttactatca gattcaacaa ttaaattaaa
     7621 tagtgcagcc tggcaatctc ctacaaaaca tttattacaa gttaaacaaa ataatggtgg
     7681 tctaactgta cagttgccaa aatcagtttc taatcaattt aaagatttgt attttgaaat
     7741 ggatttagaa ttactttcgc cggataaagc tcatgatgtt aaagtgaatg aatatacaca
     7801 agaaagaaat aaactcactt ataaatatcg acgcgttgta acacccgtaa cgatacgcat
     7861 taaagctcca gatagaatta gattatcatt gcctaaaggt aagtatcgag taaatttaaa
     7921 agggatatac ggcgaagatt ataccacgct taaagacgct tcaaattcat tagaagctgt
     7981 caaagttagt aagacaaagc atggttatac tattactaaa aataaaaatt catctgggta
     8041 tattgttttg ccaacagcat ataatcaagg tatgaaagcg acatcaggtg atcaaagtct
     8101 taaagttgaa caagtaaatg gtgttatgac cggcattaaa gcacctaaaa atataacaaa
     8161 gattcaattg agctataccc caccatacta ttatttactt ataacaatta ctatatttgg
     8221 cattatatgt agtattattt tcacgagatg ggcaagacaa aaataatgtt gctacgcata
     8281 tatttaataa atctactaag tccttttcaa tggcacttag tagatttttg tttatataac
     8341 cattttgagc aaacacgtta tgaatagaga aggaataaac aattacatta tcaactttac
     8401 attaactaaa cagcaaaaaa agccatctat atcactccat aaaagtgacc tagatggctt
     8461 tattgtttga gtcgaatttg actcatacat agctttgttt aaatgctgat taacgaattt
     8521 cttggattct agcagcttta ccacgtaaac tacgtaagta atataattta gcacgacgta
     8581 ctttaccacg acgtttaact tcgatttttt caatttttgg tgtgtgtaat gggaatgtac
     8641 gttccacgcc aacacctgat gaaattttac gaactgtaaa cgtttcagaa acgccaccgc
     8701 cacgacgttt aattacaacg ccttcgaata cttggatacg ctcacgagta ccctcaatga
     8761 tacgtacgtg tacacgtaaa gtatcaccag gacggaaact tggtaagtct gtacgtaatt
     8821 gtgatttagt tactgcttcg attaatttgt gatttgtcat tataaattct cctcttcaac
     8881 ctatgttctt gcctcgacaa atatatagca gcggatcata gtgattttag tgtttccaca
     8941 ctcagattat gttagcacaa tactaacctt ttttcaatcc tattttatat ctttctaata
     9001 tttgcttatc tgcattagtt aatggatatt tttcaattaa gtcaggtctt ttattatatg
     9061 tgcggatcaa cttttgctca tgtctccatg catcaatatt ggcatgattt ccagacaata
     9121 aaacatctgg aactgttaga cccttaaatt cacgcggacg tgtatattgc ggaaactcta
     9181 ataacccatc tgaaaatgaa tcgtcttgat gtgactgttc attacctaaa acacctggaa
     9241 tcagtctaac aatagcatca gtcatggtca tcgctggcaa ttctccacca gttaaaacat
     9301 agtcacccat tgatatttca tctgtgacaa gatgtgttcg gatacgttca tcgtaacctt
     9361 cataatgtcc gcatatgaaa acgatgtggt cggccttgct taattcaaca gctttctgat
     9421 gtgaaaatgg ctcgccttgt ggacacatta aaataacgcg tgtttgttct gtgacatcta
     9481 agtcttccat cgcattaaaa acaggttcag gctttaacac cataccttgt ccgccaccat
     9541 acggataatc atctacttgg ttgtgcttgt taattgcata atctctaaaa ttaaccgtat
     9601 tgatttgtaa tttattgttt tcttgggcac gtttcataat tgaatgattt aaaacaccat
     9661 caaacatttc aggaaataaa gttaaataat caattttcat taatccaaca atccttccat
     9721 tggcgtgatg ataatttttt tattttcaac atccacttct tttacaacat cagcaatata
     9781 aggaatcaaa tattctttag atcctttaat cacccacaca tcattcgcac ctgtttcaaa
     9841 tatattaatt acacgaccta ttggtgtttc ttgatcatca aaaactgtac atcctataat
     9901 atctgaataa taaaattcat tttcctcaag tacgatatct tcatgatcac gctcttgata
     9961 aatagaactc ccttttaaat gctcaatatc attaatatta ttaatacctt caaatgtaag
    10021 catatgaagc cctttatgca ctctatgtga cttaacagta tattcaaggt cgttattgtt
    10081 atgcacaact gtcagcactt gaccgggttg aaaacgaacg tctgtaaagt ctgaattgga
    10141 ttttacttta atttcacctt taataccatg tgtgttaaca atttgaccaa cttcaactct
    10201 cattctatta cctccaactc tatgctttga acacttctat tttaattgaa aataattcaa
    10261 agaaaaaggg caccattatg gtacccaata cgttctatat tactaacatg tgatattccc
    10321 aacgtatcat tgagtaataa ctgtcagtgt aaacgagtta ttcttattgt tagaacaatt
    10381 ttacgctaaa ttacttagct ttcttttgtt cgtcaaattt tttcataata ccttcttttg
    10441 ataagatatt gtgaactgta tcagttggtt tcgcaccatc atttaaccat tttaaagcta
    10501 acgcttcgtc aactttaatt tctggagcat tagcgctcgt tgggttataa gtaccgattt
    10561 gttcgatgat acgtccgtca cgtggagaac gagcatctgc tactacgata cgatagaatg
    10621 gatttctttt tgaacctaaa cgtgttaaac gaattttaac tgccatttat aaaatctcct
    10681 ttaagtcatg ttttaatttt tattttctac aagaaataat attaacaggt gtatgaatgt
    10741 ttgtaaagag ttttttcttt accaggaaaa ttattgaacc tttttcttac aataataact
    10801 atgtgattac taaattagac tgttaatagg ttataaaaat tcatagtaaa tttcaccacc
    10861 tacgtgttaa gttaatttat ctcgtaagga catgaatggt accacttcta ccgcacacgt
    10921 tcccccaata taacttatta atattagcaa caataacctc atgttctagc aaatcagcaa
    10981 tttcagtttt taattattat tgtgtatcta aaaatatgca ccccataaag ttatattctt
    11041 tagtataact ttcgggatgc acatctcatt gcaacaaaaa tcagcgtgtt atattaaaca
    11101 ttaaaacggt aaattcatac cttttaacat attttgcatt tgattgcgtt tacctttttt
    11161 acctttaccg ccaccagtga actgtttcat cattttcttc atatcgttaa attgtttcat
    11221 caaacgattg acttcttgta atgaacgacc agacccttta gcaatacgct ttttacgtga
    11281 tacattcaat gtgtctggat tgtttctttc agccggcgtc attgactgga taatcgcttt
    11341 aatatgatca atttgctttt cactcatatt aagcttatct agccctttca ttttattcat
    11401 acctggaatc attttcataa tatcatccag tggtcctaga tttttcacct gatcaagttg
    11461 ttctaaaaaa tcatctaaag taaacgatga ctcacgcatc tttttctcta aatcttttgc
    11521 tttttcttga tccacatctt gttgcgcttt ttcaattaaa cttaacacat cacccatacc
    11581 taaaatacgt gatgccatac gttcaggatg gaatagctct aaaccatcta acttttcact
    11641 cataccaaca aatttaattg gtttttgtgt caccgaacga atagataaag ctgcaccacc
    11701 acgtgtatca ccatctaatt tagttaaggt aacacctgtg acatcaagtt gatcgtcaaa
    11761 agattctgca acattgacag catcttgacc cgtcattgaa tcgacaacta acataatttc
    11821 gtttggttta gcaatgtctt ttacttcttt taattcgttc atcaatgctt catcgatgtg
    11881 taatcgacct gctgtatcaa tgattacaaa gtctaaatgt tcttctttag catgttttaa
    11941 tgcattagtt acaatttgtt gtggctttac ttgatctcct tcactgtata caggaatatc
    12001 aatttgtttc cctactgttt gtaattgatt tatcgctgct ggacgataaa tatctgctgc
    12061 aactaacata ggttttttgt tgtatttttt acgcatcaat aatgctaatt tacctgcagt
    12121 tgttgtttta ccagcacctt gtaaaccaac catcataaca acagtaggtg gtttatttga
    12181 catattaatc gacgtatttt ctccacccat caactgcgtt aattcatctt gaactatttt
    12241 aataacttgt tgccctggtg ttaatgattg cattacatcg gaacctaatg cgcgttctga
    12301 tactgtttta ataaattctt ttaccacttt aaagtttacg tcagcctcaa gtaacgctaa
    12361 tcttacttca cgcatcatta tctttatatc agcttcagta agtttaccct taccacgcat
    12421 tttttgcatc gtcgcttgca agcgttctga taacccttca aatgccatat cgatgtcctc
    12481 cctcaattta tatctattct aagtcttcta attgttgaat ataacgttgt atttgttctg
    12541 gattacttaa atgttgtttc atttcatcat atatttctcg gcgttgctca aatttctggt
    12601 ataattccaa tttcttttca taatcttcaa ctaaatcgcc agttcttctt atattatcat
    12661 aaactgcttg tctactcaca ttaaaagtat ctgcgatttc acttaaagaa taatcttcaa
    12721 gataaaataa ttccaaataa ttacgttgtt tattcgtcaa taaggattga taaaaatcaa
    12781 acaaataatt cattcgtaac gttttaacta aatcattttg acccatgatt atcgtccttt
    12841 tcttctgtta caatttgatc attttcaact gttgttattt cttcattttg ttcaatcata
    12901 tcagcgaata agccgtagac ataactttca gggttaaatg gttgtaagtc atctaattgc
    12961 tcacctaaac ctacatattt aactgggatg tgcaattcat tacgaatggc taatacgata
    13021 ccacctttgg ctgtaccatc taatttcgtt aatacaatac ctgtaacatt tgttacttct
    13081 ttaaagtttc tagcttgtga caacgcattc tgaccagttg tagcatctaa acatagtaat
    13141 gcttcatgag gcgcatctgg cactgctcga ttaattacac gcttaacttt ttctaattct
    13201 tgcattagat ttgttttatt ttgtaaacgt ccagcggtat cacagattaa aatatcaaca
    13261 cctttgtttt tagcggcatt aatcgcatca tacataacag cagctggatc agaaccttca
    13321 ctttggctaa ttacgtctac accaacacgt tcgccccaaa ctttcaattg atcaatagca
    13381 cccgctctaa aagtatcgcc cgcagctaac attacttttt taccttccat tttatatcgg
    13441 taagctaatt ttccaattgt tgttgtttta ccaacaccat tcacaccaac cattaaaatg
    13501 acatttaaac gaccatcttc taagttcata gcttctgaat tatcatcttc ttgatggtaa
    13561 atctctacga ttttttcaac aatgacttca cgcaaatctt cagtatcttg aatattacgt
    13621 cgttgtgctt ccatacgtaa ttcttcagtt aacgtcatca ctgtattaaa accgacgtct
    13681 gcagtgatta acatttcttc taaagcttca aaaaagtctt catctacttt acgatatctc
    13741 gctatcaaat tatttaattg ttcttggaaa ttttgacgag atttttcgag tcctgcttta
    13801 aatttagcac ccattttttg agcttcaatt tcttcaaaat cttcaattga tattaagcca
    13861 tcgtcatcaa aatccgcttc actcaacttg cgtggcttct ttttcacttc agcattttct
    13921 gctaaatcat ttgcgtcctg cgttgaacct tcagaatgtg tatcttctaa tttgtcttga
    13981 ccttgttctt ctgttaagga tttaacttct tcattttctt tatttgttgc aaacttatct
    14041 tttaagcgtt taaaaaagct cattattgct cctccttcaa cacatcatct attgtattta
    14101 aattcacact cacaagttta gtaacacctg attcttgcat tgttacaccg tataacctat
    14161 ctgcaaattc cattgttcct ttacggtgtg taataacaat gaattgtgtt tcgtctgata
    14221 actcatttaa atattttgcg tatctaataa catttgcttc atctagtgca gcttcaacct
    14281 catctaatat aacaaaaggt gcagatctta cttttaaaat tgcaaatagt aaagcaatag
    14341 cagttaatgc acgctcacca ccactcagta acgataaatg ttgcaacttt ttacccggtg
    14401 gttgtaccac aatatcaata ccagctgtta aataatcggc ttcagttaat tgcaattctg
    14461 catcgcctcc accaaacaat tgtttgaaca cagctgtaaa atgtccttga atagcatgga
    14521 aagtttcttt aaatctttca gtaacctctt gatccatttc acttataatt tgctctaatg
    14581 tttctttagc tttacgaaga tctgtacgtt gttcacttaa aaatgtataa cgttcattta
    14641 actcttcaaa ttgttcaatt gcatttaagt ttacaggacc tagttcatca atcgacatct
    14701 tcattaactt aactttttta cgtaatgcgt caatcgattc atcactcgta tattcagatt
    14761 tcgcacgttc aacagtcaat tgatattcat catttaaatg atctatcgca tgatgaatta
    14821 atacatctag ctttgattgt tcagctttaa tatcttggta gtgattttcg atagctaaaa
    14881 tatcttggtg acaaacttgt agtttagctt cttgcgcatc gatttgttca ttcaactcaa
    14941 tacgttgttg tttcaattta tctaattcat ctgataagcg tgttctcgtt tcttgttgac
    15001 cattaatttg atctttaata ttttgaaaag cttgttcgcc catcacttca tccgaattaa
    15061 agaatgcaat tttttcttta acatctttta attgatgttt agtttgttga ttttgattat
    15121 ttaatcgatc tattgtctgt tgttgtgttt taatacgctc tttaaccaca gcaagatcag
    15181 attgtttctg atgtaaggtt tgttgtgttt tagtaacgct ttccttacct tctttagaaa
    15241 gttttgtgta gcgttcaatt tcatcttcta gtcgttttaa agatgcttta atactttcta
    15301 gataagtttc tttttcactc aaagtttgtc gacttttgtc actcgtataa ccatcatttt
    15361 tttcaaattc gaattcttca tgatcatttt ttatttgtgt ttcttgtgta gttaatctat
    15421 cgagctccat ttcaaaatga tgcacttgct ctttaagtgt attatgcttt tgactttttt
    15481 caaaatacag ttcacttaat tgatcacttt ttatcttcaa ctctttaaat tgttgttcaa
    15541 atgattctgt ttgacgcaag taatcttcta attggtgtct cattgttgtc aactcgtctt
    15601 tttgagacag aatacttttt gacttacgag cgccaccacc agtcatagaa ccaccaggat
    15661 ttacaatatc accttccaaa gtaacaatac gagttcgata tttaatcgca cgtgccaatt
    15721 catttgcatg ctttaaatga tcaacgataa tcgtattacc taataaattc ccgataatat
    15781 tttgatattc tggtgctact ttaactgctt ccgaagcgat actaataaat ccgtttgcct
    15841 ctttagcaat agatttaata tcagtcgcta ccactctact ctgtataaca tttaatggta
    15901 aaaacgtcgc acgacctaaa ttacgttctt ttaaaaattg aatagcctgg cgtccatctt
    15961 tttctgaatc tacaatgaca tgttgtaatg aagcacctaa tgctgtttca attgcctgag
    16021 ttaatttaga tggcacatca ataatttccg caactgcacc ataaataccc tttaattctt
    16081 tatttttagc tttcaaaata tgtttgacgc cattgaaaaa ataagtatat tcttcctctt
    16141 gcgttgccaa actatcaata cgtgttttca ttttttcggt atatcgatat gcttgataca
    16201 atttctcttc gtattcattt tgtgcttttt ttgtatcagt gaggtctttt tctatatttt
    16261 taatttcttt atctacagca gaaagttctt tgttggtctg ttgatattct ttttttgtcg
    16321 tttttatttg accctgaata tctttcaatt gctcaaaaac ttcaactaat cgagaatcta
    16381 gtcttgattt tttagcctca ttctcttcta tagtatgctt taaaaaacga atatcattgt
    16441 taacatctga ttgctctgac attaatgtat agtattcgtt tttaatttct tccaattttt
    16501 catcatgtgc ttcgtctgaa acatatagtt gttcttcaag ttcacgaatg acagcattga
    16561 gttctttttg tttacttttc agagacttat aagtatcttg agcttcagaa atctcatttg
    16621 atatattttc taaaagctcc attaaatttt cttgttcttc ttcatatcgt gcatttgttt
    16681 cagattgatt tttcttacgt tcttccaaaa catttaattg tcccgtatat ttttcaaagg
    16741 cttccgtagc ttttactaat tgataattaa gcgattcaac atcattatca agttgatgac
    16801 gtttaccttt atattgttga atttgttggc ttaaacgttg cttgtcagct tctttatttg
    16861 cttgttggcc ttgtaaatca tttaaacgtt gatctaattg tctattgtca tttgtatatt
    16921 gatcaatatc gtgcactgta actacaatgt cactatgttt catttgatgt gaaagtgtct
    16981 tatattcttt agctatagct gcctcctctt ttagaggttc tacgcgacct tccaaatcat
    17041 ataaaatgtc ttctactctc gttaaattat cttctgtttg gtcaagttta tttaatgatt
    17101 cagccttacg ttttttatat ttaagtacac cagccgattc ttcaataatt tgacgtctat
    17161 caattggttt agcatttagt atttcatcaa ctctaccttg cgagataatg ctatacgctt
    17221 cttttcccaa tccagaatct aaaaataaat cggcaatatc ttttaatctt gcacggtcat
    17281 tatttatgta gtactcactt tcaccacttc gatacaatct tcttgttaca ataacttcgt
    17341 tttcatcaac actgagcttt ttagaatgat tatctaatct taactgtact tcagcataat
    17401 tttgagcttt gcgatgttct gcacctgaga agataatatc ttccattttt gagccacgta
    17461 atgatttagc cgattgttca cccaacaccc atttaatagc atctgtaata ttacttttac
    17521 cgcttccatt tggaccaaca attgcagtta cacctttatc gaattgaaca ttggtttgat
    17581 ctgcaaaaga cttaaatcca atggcatcta ttgattttaa ataaaccatc ctaaactcct
    17641 tatcacaatt ttaaagaaca catgtatacg atattttagt atttgtatat taaatttcga
    17701 agttgttcca taaaatatta aaaattcttc cttaaatcct aaatcgaaca cctatatgtc
    17761 gtatcatata aatttctatt taatttgttt taattgctta taggcacttt cagcagcacg
    17821 ttgttctgat tcttttttcg ttttcccttt accttcagct attgcttccc cttgcagaat
    17881 aacttctgaa gtgaatagac gatgatgtgc cggtccctct tcttttatta aattataggt
    17941 tacatcacct ttattttgct ggtgcacata ttcttggaat tgtgttttaa aatctaccac
    18001 gcctaataac tcattttgtt ctacatgtgg gaaaatgact ttctcagcaa atttccaaac
    18061 tatatctagt ccttgatcca aatacaatgc cccaataaat gcttcgaatg catctgatat
    18121 taatgatggt cttgtacgtc cccctgtttt ctcttcacct ttaccaagta aaatcatttc
    18181 gttcaatcca attttattcg caaatattac aagtgagggc tcacatacaa tagtggcacg
    18241 catttttgtt aaattccctt ctggcaagtt gggatgttta tcaaataaat atcgtgaaac
    18301 cgtcaattct aataccgcat cacccaaaaa ctctaaacgc tcattatggt ctaaacgatt
    18361 catattaaaa tcattaataa aactcgaatg cgaaaatgct tgttggtata aatcaatatt
    18421 ttgataagta aagcctaact ctgtcatttt agtatcaaag cgctttctaa aacgattaac
    18481 tatctcactt ttcttttgtt tagacattat tttatctcct ttatgtgttg ctcttgaatc
    18541 atctttaatt ttacgttaaa actattgata attaaagaaa aaaactgagt cgacaatact
    18601 gacgacccag atgtaagatt tattattttt caagactgtt aataaattta acagcatcac
    18661 caacagtgtt gattttttca gcttcttcat caggaatttc agtaccaaac tcgtcttcta
    18721 attccattac taattcagcg atatcaagtg agtcagcgcc taaatcatct ttgaaagatg
    18781 catcttcagt tactttatca gcgtctacac ctaaacggtc aacgatgata tcttttactt
    18841 tatcgaaatt ttccacgtcg attcacctcc tttaaaaatg cctatacata gactactata
    18901 ttttcccatt ttaaattcta aaaaacaagt agaaatttta gaaacactta tcaatttatt
    18961 gattagtgtt tcttagggag aaattctgga accttatcta ggttgcttag aatttccccc
    19021 gcaaagttga ctaggtcaga caattctcca ttggatgacc agtcaaccac tgcgtcaatg
    19081 agctttagct caaatatatt acatgtacat tccaccattt acatggattg tttgacctgt
    19141 aatatatttt gctttgtctg atgctaagaa cgctactgta ttagcaatat ctgtgtcttg
    19201 accaaaacgt gctaacggaa tttgagtcaa catttgttct ttaagctcat cacttaaagc
    19261 atctgtcata tcagaaacaa taaaaccagg tgcaactgca tttacagtga taccacgaga
    19321 tgctaattca cgcgccgcag atttagttaa accaataaca cctgcttttg ttgcaacata
    19381 gtttgcttgt cccggattac ctactgctcc aacaacactt gataaattga tgatagcacc
    19441 actacgttgt cttaacattt gtggtgttgc tttttggata cagttaaata caccttttaa
    19501 gtttgtgtca ataacatcat cccactcttg ttctttcata cgcattaata aattatcgcg
    19561 agtaatacct gcattattta ctaaaacatc taaagaacca aattggctaa ctacttcttt
    19621 aatcattgct ttaacttcat cagcatcggc aacatttgct tgaatcgcaa aactgtcaac
    19681 acctttagct ttgatttctt cgactactgc ttcagctttc tctttgctgc ctgcatagtt
    19741 tactgctaca ttatatcctt cttctgctaa ttgtaacgca atactacgtc caattcctct
    19801 tgatgcacct gttactaaag cactcttagt cattttcatt ccatcctttc acatcttcta
    19861 aagtttgaat tgatgttaac ttaacatctc tatttatttt tttaattaag ccagataaaa
    19921 cttttccagg accaatttca ataaaatgat caacaccttg gtctattagc cattctgttg
    19981 agttaatgaa ttgtactggt gaatataatt gcttgaccat attagattta attacttctt
    20041 tgtcagtttc accttgcgca tttacatttt gaactacagg aaacttagca tcacgccatt
    20101 caaattgatt aatgtaactt gaaaaatctt cttcaatcac tttcattagc gatgaatgga
    20161 atggtccaga tactgctaaa ggcatgacac gttttgcacc taatgattta cctttttcta
    20221 ctagctcatc aattaaagct ttgtgacctg aaacaacaat ttgacctggg caattaatgt
    20281 ttgctggttc aattattttg tcatcagatg ataatgactt acaaatttca tcgactttat
    20341 caaaatctaa tcccaatact gcagccatgc ttcctacacc agtaggaaat gcttgcgcca
    20401 ttaattgacc acgttttcta acaattttaa ctgcatcttc aaatgataat acgtcagctg
    20461 caactaaact tgaatattca cctaaactat gccccatagt aaaatcagga ttcaaatttt
    20521 ttagcgctgc taataatgcc gaactatgcg tcaataaagc tggttgtgtg ttttcagttt
    20581 cacccaattt accttcttca tcagtaaaca ttgtctctaa aatatcaaag tctaatgtgt
    20641 tcgctgctga agttaaaatt tcagttgctt gatcattgtt gttaaacaaa tcttgcgcca
    20701 caccaacttt ttgggcacct tgtcccggaa aaataattgc tgttttactc atttgattca
    20761 cctacagtct ctttcattgt ttgtacaata ttttgttctc ctgcgatttt cgcttgtcta
    20821 attgcagaat aaaaagcttt agcatttgaa ctaccgtgtg ctttaactac agtaccttcc
    20881 aatcctaata atacggaacc accgtattct gagtaatcca tctttttagc gaattcagct
    20941 aaatctttct tcaatattgc acctgctaat ttatttttag tactactcat aatcgtatct
    21001 tttaacattt taccgattga ttttgcagta ccttctaaat ttttaaggac catgttccca
    21061 gtatagccat cggtaactac aacatctgta tcgccatcca ttaatgtctt cgcttcaata
    21121 ttcccaacaa aattcaatga atgatcatga tttaataact catatgattt tttcgttaaa
    21181 ctattacctt tagctggctc ggttcctata tttaataatg agattttcgg attatcaata
    21241 cctctaattt tttgagcata aatatcccct agttgcgcat actgtaataa gtgttcaggt
    21301 ttagcatcag catttgcacc aacgtctaaa aagacaaaac cttttccatc aatcgttggc
    21361 aatgttacta ctaaagccgg tctagctaca cctttaatac gtccaacaat gaataaacca
    21421 gctgacatta aagcaccagt attacctgct gacacacatc catctgcttc accagatttc
    21481 acagcttcag ccatttttac cattgagcta tcttttttac gtttaatcgc tctaacaggc
    21541 tcatcttcca tttcaatctt ttcagaacaa tgtctaaatt cgattcgttc atggttcaga
    21601 ttatactttt tttcgtcacc gaaaagtata atttctagat ctttaaagtc ttcaacagcc
    21661 ttttgtacgg cttctaatac gatatcaggc gcattgtcgc cacccatcat atcaattgct
    21721 aatttaacca taattttatc ctcgcttatc ataaaacatt ttaaaatttc ctttgaaaac
    21781 taatgtatgt ttaacatatg actttacttc gacgtaataa tgttttgcag tttgatttac
    21841 aactcgtgct tctgctctta ccgtatcatt taattttact ttttcaataa attgaatgct
    21901 actctcatga gttaaaactg ttggttgctt aattagcgca acacataacg aatttgcctg
    21961 agcaaacagc acatgaccac gcgcaattcc agttttatga aaaacagaat ccgatgtaat
    22021 atctaaaatt gattgcgctt taacatttgg attgacttga atcaaatcac caataaattc
    22081 ttgttcttca atagaactta tttggtcata attcttttca gcaactaatt taatacgctt
    22141 ccttaattct ggtatgttta aataagtgcg atctaaacga attgtttgta tactcacttg
    22201 aaataagtcg cttagttcat ggtctgtgat gaagggattg ctatcaattt gttgtctgat
    22261 tgcttctcta cgtttatctt tctttagttt caacgtctca cccctcattt tttagtacct
    22321 agtcttaaac attcctcaca tatcataaca ctttctattt aaccgactca acaaataact
    22381 gtaatattta gctattattt agtcgaaaat ttataaattg ttaaaatacc actttacatt
    22441 taaatagttc aagtgtcata tcactcaatt aacgaagtgt tgcactcaaa ttgacaaatc
    22501 agcatggcaa ttagtcaaaa ctacgatgta ataaattttc ttcaacaaaa tgacgtaaat
    22561 gttgatacgt attttcaaag aatacgccag attgaataag ttcagctgct tcatcacgag
    22621 caacttctaa catacgataa tcttcaacta aattggcaac taagaaatct ggcaatccac
    22681 tttgtttaac accaaagaaa tctccaggac cacgcatttc taagtctcgt tcactcaatt
    22741 caaatccatc cgttgtttgt gtcataattg tcattctttc aattcctgtt tctgttttag
    22801 gggatgcaat taaaacacag taactttgct ggtcacttct acctacacga ccgcgtaact
    22861 gatgtaaagt tgataatcca aagcgatccg catcataaat catcataaaa gttgcattcg
    22921 gtacattaac acctacttca acaacagtag tagaaactaa aacatttatc tcatgattac
    22981 taaacttttg catgacctca tctttttcat cggcagataa cttaccatgc aataacccta
    23041 cacgggaaac accataatac tgttgtaaag actcgtacaa tgcgacaaca ttttgaacat
    23101 cttcgagatg ctcagaactt tctattagcg ggcaaatgac atatgcttga cgaccttttt
    23161 tcaactctga ggtcatttgc atcaaaactt tatcgtattg ctcatgcttt gcccaagtag
    23221 taatgatagg tttacgacct tttggtaatt gtttaattga agacacatcc atctcaccaa
    23281 aaactgatat tgctagtgtt cttggtatcg gcgttgctgt cataaataac acattcgtca
    23341 ttgcaccttt ttctcttaaa agctggcgtt gattcacacc aaatcgatgt tgttcatctg
    23401 taattactaa accaacatta tggaaaatca catcatcttg aatcaaagca tgggttccaa
    23461 ttaaacaatc aatcgtacca ttttcaagtt gttctaaaag tatctttcgt ttcttacctt
    23521 ttactgaccc agttaacaat gcaacgttca tagaatctcc aaataaagcc attaaacttt
    23581 cagcatgttg ctctgctaaa atttcagttg gtaccatcaa tgctgattga taaccagcag
    23641 tttttaacgc atacatacaa attgcagcaa ctactgtttt tcctgaacct acatcacctt
    23701 gaagtaatcg atgcatacgt attggtgctt ttaaatctct aaaaatttca ttaacactgg
    23761 atttctgtgc ttcagttagt tcaaaaggta aacgatcaat aaatgattta acttggtcta
    23821 tgtcataatc aacttcaatt gcttcgtcag atgacttttc taatctattt agccattgca
    23881 tacgtaattc gaataaaaac agttcagtaa atgcataggt tctacgagca cgtaataaat
    23941 cctctttact tttaggatga tgtaatgtgt tcaaagtaaa gtccaaggtc tctaatttat
    24001 atttttctct tagttcatca gttaaccatt catgaattgt cacatcattt aacgcttgtc
    24061 taatttggtc tcgtatttgc ttttgtttaa taccttcctt aatacgatag actggttcta
    24121 attgaacatc tgcgttttct tgagtttgtg tcccttgtga attaaagaaa accctattac
    24181 cagtaatttc ctgtttaacc ctattccact tacctttaac agttatagtt tgatttaatt
    24241 cgattttctt ttttaaatac ggttgattga aaaaaataca tttgacagca atattattta
    24301 ccattaaatg aacggttaat tttgatttat ttcttccaaa aaatgcaact actggagctg
    24361 tatatacttg tccttctatc gtaacgttag attgatcttc tgcttgattc aaatcaatca
    24421 ctgtattatc ttcatatcta gttggcaaat aaagaacaag atcttccact gtatgaatat
    24481 ttagttgttg caatacttct attttcttag gacctatacc ttttaattgt aaaagagaat
    24541 atggactttc tattaagttt actttagcca aaataaatca cctatgtttt atttgatttt
    24601 acgtatcatt tagtatgtta tttattttat catgactaag ttgaacatga cattgttagc
    24661 aactcgagaa cgatactttt aacccgcaat atcactaaaa tagcaaaata aaaaactacc
    24721 aactgatgat ttatcattgg tagtttttta ttttaaattt ttattctact gaaaagaaat
    24781 attgataaat tggttgtcca ccttcatgaa cttccacttc tacatctgga tattgctctt
    24841 cgatccagtt tatcatgtta tctgtaactg cttgctctgc atcttgacca ataatcacag
    24901 tcaatatttc actatcttct gctaacatct catttaacaa ctcagtaacc gttgttaatt
    24961 gatcactttg gctgcttaca atcttatctt caatcaagcc cataaacgcg tcttttttaa
    25021 tctcaacgcc atcaattttc gtatcacgaa cagcgtacgt taatgaacca gatttaacgt
    25081 tatttactga atcagccatt tgcgctttat tttcttcaag tgttgcgtcc acatcatatt
    25141 ggaatagtgc gcttatacct tgaggaatag atttcgttgg aataacaaca gcttctgcat
    25201 caacaatact cgctgcttgt tcacttgcca ttaagatatt tttattattc ggtaaaataa
    25261 ttgcacgttt acattttgat tgttcaatga ctttaacgat atcttctgta gaaggattca
    25321 tcgtttgtcc accactaatg atatgtgtgg cacccattga tttaaatatc tctgaaatac
    25381 cttcacccat agaaatagta ataattgctg tttcaaccgt ttccattttc ggtttagctg
    25441 tgtgctgttc ttttcgaatc acttcacggt gctgttctct catattttca accttaagtt
    25501 taattaattc accatattgt tgaccataat taaacacttt acctgggtat tcggtatgca
    25561 cgtgaacttt cacaatttct tcatcattaa tgactaataa agaatcacca aattgactca
    25621 tatcttgcct gaattcttgt tcatcaaagg cttttttatt ctttccaaaa cgaaccatca
    25681 tttcagtaca atagccataa ataatatctt cagtattaat tacaccatgg aaatcatgtt
    25741 catcatgtac aaattcatct ttatctatct ttgcaacttt ggcttcaact ttttcacctt
    25801 taagcgcttt taagaatcct tcgtaaacgc ataacaaacc tttaccgcca ctatcaacaa
    25861 caccaacttc tttaagtaca gctaataagt ttggtgtgtt ttcaagtgat tcattggctt
    25921 ttacaataat gtactccatt aattctatac aatcttcagt attatttgct ttttctattg
    25981 cagcttgcgc agcatctttt gcaactgtaa gtattgtacc ttcaactggt ttcataacag
    26041 ctttatatgc cgtttcaaca ccagcttgaa aactttcagc taacaatttt gaattaattt
    26101 cagattcact ttcaatattt ttacaaaatc ctctgaataa ttgtgacaag atgacaccag
    26161 agttacctct tgcacccatt agtaaacctt tcgagaatgt tttacctaat tcgccgatat
    26221 ttttcgacaa attattctct acttcttcgc gacctgaagt catagtaaga ttcatatttg
    26281 ttcctgtatc accatctggc actggataca cattcaaaga atctaccaaa tctgcattgt
    26341 tagataaatt ttgtgcccct tgtataatca tatcggcaaa taatttacca ttaattttgc
    26401 taatcatttc aagttgtcct cctaagcttt cttgcctgta ttattcacac gtacaccttg
    26461 tacatatata ttgattgaat ttactgatac attaagtgat ttttccaaag tatatttcac
    26521 tgttgattgt acattattgg caacttcaga tattttcaca ccgtaactta caataatgta
    26581 catatctata tccactacgc cattattttc agttactttg atgcctttag cataattttc
    26641 atgtcctagt atttccgcaa taccatctct aacttgttgt ctagatgcca tacctacaat
    26701 accataacat tcaacggcct ttccacctac aaccgaagca atcacttcgt ttgaaatatc
    26761 aattttaccg taatcatttg aaatctctaa tgtcatatct ttgtccctcc taaatttcaa
    26821 agtttattat tcgttttccc taatcacttt taaaatttta ccacttagtg tagtcataaa
    26881 cgctttaagt ttcattgatt atttttataa aatatcaatg gtacaactta tcagaatga
//
LOCUS       DAXWFK010000014        26761 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.014, whole genome shotgun sequence.
ACCESSION   DAXWFK010000014 DAXWFK010000000
VERSION     DAXWFK010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 26761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26761
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.014"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            68..1027
                     /locus_tag="ACGEXS_000493"
     CDS             68..1027
                     /locus_tag="ACGEXS_000493"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFF8011540.1"
                     /translation="MKTFGKKVVLIGDGSVGSSYAFAMVTQGVADEFVIIDIAKDKVK
                     ADVQDLNHGTVHSPSPVDVKAGEYEDCKDADLVVITAGAPQKPGETRLQLVEKNTKIM
                     KSIVKSVMDSGFDGYFLIAANPVDILTRFVKEYTGLPAERVIGSGTVLDSARLQYLIS
                     QELGVAPSSVDASIIGEHGDTELAVWSQANVAGISVYDTLKEQTGSEAKAEEIYVNTR
                     DAAYEIIQAKGSTYYGIALALMRISKAILNNENNVLNVSIQLDGQYGGHKGVYLGVPT
                     LVNQHGAVKIYEMPLSAEEQALFDKSVKTLEDTFDSIKYLLED"
     gene            1305..2009
                     /gene="budA"
                     /locus_tag="ACGEXS_000494"
     CDS             1305..2009
                     /gene="budA"
                     /locus_tag="ACGEXS_000494"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFF8011541.1"
                     /translation="MTNVLYQHGTLGTLMAGLLEGTATINELLEHGNLGIATLTGSDG
                     EVIFLDGKAYHANEHKEFIELKGDEKVPYASITNFKASKTFPLQQLSQDDVFAQIKNE
                     MLSENLFSAVKIYGTFKHMHVRMMPAQQPPYTRLIDSARRQPEEKRQDIRGAIVGFFT
                     PELFHGVGSAGFHIHFADDERAYGGHVLDFEVDDVVVEIQNFETFQQHFPVNNETFVK
                     AKIDYKDVAEEIREAE"
     gene            complement(2095..2955)
                     /locus_tag="ACGEXS_000495"
     CDS             complement(2095..2955)
                     /locus_tag="ACGEXS_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFF8011542.1"
                     /translation="MLSVAIIGPGAVGTTIAYELQQSLPHTTLIGRHAKTITYYTVPH
                     APAQDIVVKGYEDVTNTFDVIIIAVKTHQLDAVIPHLTHLAHEDTLIILAQNGYGQLE
                     HISFKNVCQAVVYISGQKKGDVVTHFRDYQLRIQDNALTRQFRDLVQDSQIDIVLEAN
                     IQQAIWYKLLVNLGINSITALGRQTVAIMHNPEIRTLCRQLLLDGCRVAQAEGLNFSE
                     QTVDTIMTIYQGYPDEMGTSMYYDIAHQQPLEVEAIQGFIYRRAREHNLDTPYLDTIY
                     SFLRAYQQNM"
     gene            3028..3846
                     /gene="panB"
                     /locus_tag="ACGEXS_000496"
     CDS             3028..3846
                     /gene="panB"
                     /locus_tag="ACGEXS_000496"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFF8011543.1"
                     /translation="MKTVSQLIDMKQKQTKISMVTAYDFPSAKQVEAAGIDMILVGDS
                     LGMTVLGYESTVQVTLADMIHHGRAVRRGAPNTFVVVDMPIGAVGISMTQDLNHALKL
                     YQETNANAIKAEGAHITPFIEKATAIGIPVVAHLGLTPQSVGVMGYKLQGATKEAAEQ
                     LILDAKNVEQAGAVALVLEAIPNDLAEEISKHLTIPVIGIGAGKGTDGQVLVYHDMLN
                     YGVEHKAKFVKQFADFSVGVDGLKQYDQEVKSGAFPSEEYTYKKKIMNEVNNND"
     gene            3839..4690
                     /gene="panC"
                     /locus_tag="ACGEXS_000497"
     CDS             3839..4690
                     /gene="panC"
                     /locus_tag="ACGEXS_000497"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFF8011544.1"
                     /translation="MTKLITTVKEMQHIVKAAKRSGTTIGFIPTMGALHDGHLTMVRE
                     SVSTNDITIVSVFVNPLQFGPNEDFDAYPRQIDKDLELVSEVGADIVFHPAVEDMYPG
                     ELGIDVKVGPLADVLEGAKRPGHFDGVVTVVNKLFNIVMPDYAYFGKKDAQQLAIVEQ
                     MVKDFNHAVEIIGIDIVREADGLAKSSRNVYLTEQERQEAVHLSKSLLLAQALYQDGE
                     RQSKVIIDRVTEYLESHISGRIEEVAVYSYPQLVEQHEITGRIFISLAVKFSKARLID
                     NIIIGAE"
     gene            4692..5075
                     /gene="panD"
                     /locus_tag="ACGEXS_000498"
     CDS             4692..5075
                     /gene="panD"
                     /locus_tag="ACGEXS_000498"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015901315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFF8011545.1"
                     /translation="MIRTMMNAKIHRARVTESNLNYVGSITIDSDILEAVDILPNEKV
                     AIVNNNNGARFETYVIAGERGSGKICLNGAASRLVEVGDVVIIMTYAQLNEEEIKNHA
                     PKVAVMNEDNVIIEMIHEKENTIVL"
     gene            complement(5204..6886)
                     /locus_tag="ACGEXS_000499"
     CDS             complement(5204..6886)
                     /locus_tag="ACGEXS_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HFF8011546.1"
                     /translation="MNQHLLGNPKLTVTHVNEVKAGINHIVVDSVQYGNQEMIMEKDV
                     TVEMRDGEKLYINIFRPNKDGKFPVVMSADTYGKDNKPKITNMGALWPTLGTIPTSSF
                     TPEESPDPGFWVPNDYVVVKVALRGSDKSKGVLSPWSKREAEDYYEVIEWAANQSWSN
                     GNIGTNGVSYLAVTQWWVASLNPPHLKAMIPWEGLNDMYREVAFHGGIPDTGFYRFWT
                     QGIFARWTDNPNIEDLIQAQQEHPLFDDFWKQRQVPLSQIKTPLLTCASWSTQGLHNR
                     GSFEGFKQAASEEKWLYVHGRKEWESYYARENLERQKSFFDFYLKEENNDWKDTPHVI
                     YEVRDQFYKGEFKSASAFPLPNAEYTPLYLNAENHTLNHAKISSAHVAQYDSEDKQQD
                     VSFKYTFDKDTELVGNMNLKLWVSTKDSDDMDLFAGIKKLDRRGNEVNFPDFNHIENG
                     QVATGWLRVSHRELDQEKSSIAQPWHKHETELKLSQDEIVPVEIELLPSGTLFKQGET
                     LEVVVKGSEIVIGNSTPGMKTRYEHEETVNKGMHMIYTGGKYDSQLIIPIVN"
     gene            6949..7041
                     /locus_tag="ACGEXS_000500"
     CDS             6949..7041
                     /locus_tag="ACGEXS_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011547.1"
                     /translation="MINVGDLWDNEHNVYNHWGGLDINDYRSTY"
     gene            7019..7606
                     /locus_tag="ACGEXS_000501"
     CDS             7019..7606
                     /locus_tag="ACGEXS_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8011548.1"
                     /translation="MTIDRRIRKSQVAMRNAFIELLHQHQLEEITVQQIADLADVNRS
                     TFYTHYYDKYDLLEKLEDQQLEEIRDFIHDEKLNGAVKLSTDNIHQIMTVLIEMIEKD
                     IAFYQLMFRMGKDSNIHEKLYDLIMCHLQRYKNENDQINGIPFSYFMSYVSGAGLSFI
                     RHWVEDPNRIDKVDLIRHFNTIVNQGPATIIKNIQ"
     gene            complement(7687..8136)
                     /locus_tag="ACGEXS_000502"
     CDS             complement(7687..8136)
                     /locus_tag="ACGEXS_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFF8011549.1"
                     /translation="MTALFPYIAFENSKEALAYYEEVFGATDVKRLEVGEEQASHLGM
                     TKEEAQEATMHAEFEVLGVKVLCSDSFGRADKINNGISLLIDYDVNNKEDADKVEAFY
                     EQIKDHSSIEIELPFADQFWGGKMGVFTDKYGVRWMLHGQDYTAIQQ"
     gene            8490..9212
                     /locus_tag="ACGEXS_000503"
     CDS             8490..9212
                     /locus_tag="ACGEXS_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFF8011550.1"
                     /translation="MINAEPIISKMKNQKINYDKVLKKLIGQWEREAIRPKILLHSCC
                     APCSTYTLEFLTQYADIAIYFANSNIHPKNEYLRRAKVQEQFVEDFNRKTGANVKYIE
                     APYEPHKFVKMVKDKELADEKEGGLRCTACFEMRLDIVAKAAVEHGYDYFGSAITLSP
                     KKNAQLINELGMDVQKIYDVNYLPSDFKKSKGYERSIEMCNDYNIFRQCYCGCVFAAM
                     QQGIDFKTVNKEAKAFLEQYPD"
     gene            complement(9398..9673)
                     /locus_tag="ACGEXS_000504"
     CDS             complement(9398..9673)
                     /locus_tag="ACGEXS_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011551.1"
                     /translation="MTKETDNKKTVATSDNKDTQQQNQPEEDERQGLNGYRKTDLDLE
                     IEQELREMMKTGENETKNDYKKFKVFSLISTLVIVILAIIRFVHKMM"
     gene            complement(9828..10892)
                     /locus_tag="ACGEXS_000505"
     CDS             complement(9828..10892)
                     /locus_tag="ACGEXS_000505"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFF8011552.1"
                     /translation="MYKLIKPFLFKIEPEKAHGLTIDALKTLQKFPVLFPVVDKLFTY
                     KNPTLSQTIQGNTYDNPIGLAAGFDKSCEVPKALEHLGFGALELGGITPKPQPGNPQP
                     RMFRLLEDDALINRMGFNNIGMNKALSHLRKNAYQVPVGINVGVNKMTPYEARYQDYI
                     KVIDTFKHDVSFFTVNISSPNTENLQNFHDKDEFSMLCQALTAFKKQHDVTVPIYLKL
                     TSDMDFDGLKALLPAITETFDGIILANTTRQRDGLTSANKVEEGGLSGRPLFERNLKL
                     IKYAYQQTNGEFLIIGTGGVFSTEDAIKMMRHGASLIQIYSSLVIEGPGLTKKMNKGI
                     ARYLKDHHFDNVSDIIGLDA"
     gene            11110..11976
                     /locus_tag="ACGEXS_000506"
     CDS             11110..11976
                     /locus_tag="ACGEXS_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFF8011553.1"
                     /translation="MNEQWLEHLPLKDIKEISPVSGGDVNEAYRVETDTDTFFLLVQR
                     GRKESFYAAEIAGLNEFERAGITAPRVIASGEVNGDAYLVMTYLEEGASGSQRQLGQL
                     VAQLHSQQQEEGKFGFSLPYEGGDISFDNHWQDDWCTIFVDKRLDHLKDELLNRGLWD
                     ANDIKVYDKVRRQIVAELEKHQSKPSLLHGDLWGGNYMFLQDGRPALFDPAPLYGDRE
                     FDIGITTVFGGFTSEFYDAYNKHYPLAKSASYRLEFYRLYLLMVHLLKFGEMYRDSVA
                     HSMDKILQDTTS"
     gene            12318..12596
                     /locus_tag="ACGEXS_000507"
     CDS             12318..12596
                     /locus_tag="ACGEXS_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04197 family type VII secretion effector"
                     /protein_id="HFF8011554.1"
                     /translation="MMSTVQSDIFKTNSASSSIKSAVETCNNVSKPDKDESTTVSGNN
                     NAHSVIDDLMSKNQSVAEAIRTASDNIQKVGEAFDQTDVMIGNEIGKN"
     gene            12611..12979
                     /locus_tag="ACGEXS_000508"
     CDS             12611..12979
                     /locus_tag="ACGEXS_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011555.1"
                     /translation="MSNKLDEINKIITAKHEQMDDLYDEKREVKALIDESDALNHSID
                     QLYQHLGERYYSSNMASRMEQFRDEFHFAKRRSTEALYEQQQQIQHGIRKVEEEMIDL
                     EMRRNVEIETVTKEENKWKQ"
     gene            12967..13263
                     /locus_tag="ACGEXS_000509"
     CDS             12967..13263
                     /locus_tag="ACGEXS_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4176 domain-containing protein"
                     /protein_id="HFF8011556.1"
                     /translation="METIGSIIYLKEGSQKLMIINRGPIVEIENQKYMFDYSACKYPI
                     GVVEDEIYYFNEENIDSVIFKGYSDQDEVRFQELFENMKQNLDSEIQRGEVTQQ"
     gene            13597..14703
                     /locus_tag="ACGEXS_000510"
     CDS             13597..14703
                     /locus_tag="ACGEXS_000510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFF8011557.1"
                     /translation="MQHHKVAIIGAGAAGIGMAITLKDFGITDVIILEKGTVGHSFKH
                     WPKSTRTITPSFTSNGFGMPDMNAISMDTSPAFTFNEEHISGETYAEYLQVVANHYEL
                     NIFENTVVTNISVDDAYYTIATTTEIYHADYIFVATGDYNFPKKPFKYGIHYSEIEDF
                     DNFNKGQYVVIGGNESGFDAAYQLAKNGSDIALYTSTTGLNDPDADPSVRLSPYTRQR
                     LGNVIKQGARIEMNVHYTVKDIDFNNGQYHISFDSGQSVLTPHEPILATGFDATKNPI
                     VQQLFVTTNQDIKLTTHDESTRYPNIFMIGATVENDNAKLCYIYKFRARFAVLAHLLT
                     QREGLPAKQDVIENYQKNQMYLDDYSCCEVSCTC"
     gene            14697..16037
                     /locus_tag="ACGEXS_000511"
     CDS             14697..16037
                     /locus_tag="ACGEXS_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFF8011558.1"
                     /translation="MLEVKYDMRTGHYTPIPNEPHYLVISHADKLTATEKAKLRLLII
                     KQKLDISLAESVVSSPIASEHVIEQLTLFQHERRHLRPKISATFLAWLLIFLMFALPI
                     GIAYQFSDWFQNQYVSAWIEYLTQTTLLNHDILQHILFGDYGVLSLGTYSLVWALPVV
                     ILISLSTAIIDQTGLKSWMIWAIEPSMLWIGLQGNDIVPLLEGFGCNAAAISQAAHQC
                     HTCTKTQCMSLISFGSSCSYQIGATLSIFSVAGKSWLFMPYLILVLLGGILHNRIWLK
                     KNDQQLSVPLPYDRQLHMPNIRQMLLQMWQNIQMFIVQALPIFITICLIVSILSLTPI
                     LNVLSQIFTPILSLLGISSELSPGILFSMIRKDGMLLFNLHQGALLQGMTATQLLLLV
                     FFSSTFTACSVTMTMLLKHLGGQSALKLIGKQMVTSLSLVIGVGIIVKIVMLII"
     gene            16135..17025
                     /locus_tag="ACGEXS_000512"
     CDS             16135..17025
                     /locus_tag="ACGEXS_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFF8011559.1"
                     /translation="MKIVIIGGFLGGGKTTVLNHLLAESLKESLKPAVIMNEFGKMSV
                     DGALVSEDIPLSELTEGCICCAMKADVSEQLHQLYLKEQPDIVFIECSGIAEPVSVLD
                     ACLTPILAPFTTITHMIGVIDASMYKHIKSFPKDIQGLFYEQLAYCSVLFVNKIDSAD
                     VETTSKLLKDLEVINPEADIQVGMHGSVTLPISVRQMTATSDNKHKSLHQMINHQFVQ
                     SPVKCTKAEFIKRLACLPSHIYRLKGFMTFEDTAHTYLIQFTQGQYELTPVAFSKKVP
                     EYLVLIGKGISKEDYQCLEQ"
     gene            complement(17172..18002)
                     /locus_tag="ACGEXS_000513"
     CDS             complement(17172..18002)
                     /locus_tag="ACGEXS_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFF8011560.1"
                     /translation="METLELQGAKLRYHQVGQGPVLIFIPGANGTGDIFLPLAEQLKD
                     HFTVVAVDRRDYGESELTEPLPDSASNPDSDYRVKRDAQDIAELAKSLSDEPVYILGS
                     SSGSIVAMHVLKDYPEVVKKIAFHEPPINTFLPDSTYWKDKNDDIVHQILTEGLEKGM
                     KTFGETLNIAPIDAKMMSQPADTEEGRIEQYKRTMFWSEFEIRQYTHSDITLDDFTKY
                     SDKITLLNGTDSRGSFPQDVNFYINKETGIPIVDIPGGHLGYIQKPEGFADVLLNMWG
                     "
     gene            complement(18022..19032)
                     /locus_tag="ACGEXS_000514"
     CDS             complement(18022..19032)
                     /locus_tag="ACGEXS_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFF8011561.1"
                     /translation="MKSITFEEHYVIEDIQKETMNAISADPKGVPMKVMLEGLEKKTG
                     FTNADELSHHDERIQFMNNQDVQIQVLSYGNGSPSNLVGQKAIELCQKANDQLANYIA
                     QYPNRFVGFATLPINEPEAAAREFERCINDLGFKGALIMGRAQDGFLDQDKYDIIFKT
                     AENLDVPIYLHPAPVNSDIYQSYYKGNYPEVTAATFACFGYGWHIDVGIHAIHLVLSG
                     IFDRYPKLNMIIGHWGEFIPFFLERMDEALFAEHLNHPVSYYFKNNFYITPSGMLTKP
                     QFDLVKKEVGIDRILYAADYPYIEPEKLGVFLDELGLTDEEKEKISYTNGAKLLGLSP
                     NN"
     gene            complement(19130..19834)
                     /locus_tag="ACGEXS_000515"
     CDS             complement(19130..19834)
                     /locus_tag="ACGEXS_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFF8011562.1"
                     /translation="MTKIVLITGGNKGLGYASAEALKALGYKVYIGSRNDVRGQQASQ
                     KLGVHYVQLDVTSDYSVKNAYNMIAEKEGRLDILINNAGISGQFSAPSKLTPRDVEEV
                     YQTNVFGIVRMMNTFVPLLEKSEQPVVVNVSSGLGSFGMVTNPETAESKVNSLAYCSS
                     KSAVTMLTLQYAKGLPNMQINAADPGATNTDLVGDFSNNSKHVSEGIKPIIQLATIGA
                     DGPTGTFINGDGEMPW"
     gene            complement(19827..20384)
                     /locus_tag="ACGEXS_000516"
     CDS             complement(19827..20384)
                     /locus_tag="ACGEXS_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8011563.1"
                     /translation="MRKDAKENRQRIEEIAHKLFDEEGVENISMNRIAKELGIGMGTL
                     YRHFKDKSDLCYYVIQRDLDIFITHFKQIKDDYHSNYEVMQVSLDYLLQFKIDNKALL
                     QCIEAGNNKLRFYQSAFYQELFDFYYDLFKSDDDTYTKFKTDMLLQSLSTSVFAFQIE
                     HRHISIEAYRNYLLNIYLDEVGRND"
     gene            20515..20814
                     /locus_tag="ACGEXS_000517"
     CDS             20515..20814
                     /locus_tag="ACGEXS_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2316 family protein"
                     /protein_id="HFF8011564.1"
                     /translation="MSLNKEQRRITAEELQAHFEASTLSVQMIAEKLNVTTEDVEKVL
                     AMTAPLGIFSHQLQRFIHLVWDVRDVINDNIKGNGQTPEPYTYLKGEKEDYWFLR"
     gene            20834..21655
                     /locus_tag="ACGEXS_000518"
     CDS             20834..21655
                     /locus_tag="ACGEXS_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HFF8011565.1"
                     /translation="MKDILVIGATGKQGNAVVKQLLEDGWYVSALTRNKNNRKLSDIG
                     HPHLSIVEGDLSDNVSLQSAMKGKYGLYSIQPIVKDDVSEELRQGMKIIEIAEQENIQ
                     HIVYSTAGGVNRNRTGPHFEVLAKIENRLMESNINATVIKPSFFMDNFLRIAKVEDER
                     ITLPEFINPNIKFTMISSIDIAKIASYIFAHPQSFTHQSIEIGSDEVTLSEAATIFSE
                     VTGKSTVIEGEFVSGVAEKQWLEEKGYEVDFELMAEINPTRLSLSDWLKVQNYNK"
     gene            21672..22049
                     /locus_tag="ACGEXS_000519"
     CDS             21672..22049
                     /locus_tag="ACGEXS_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFF8011566.1"
                     /translation="MIQSMWFNLHVQDLEKSAQFYKALGFKINRNPQMLDKMVGIQIG
                     QTTVILIENKHFQNVSQQSLNTEPNEVMISLGVNTNEEVDQLVNKVKEAGGTVVQEPT
                     VSQGFYGAMFKDLDGHHFNFLVC"
     gene            complement(22199..22429)
                     /locus_tag="ACGEXS_000520"
     CDS             complement(22199..22429)
                     /locus_tag="ACGEXS_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HFF8011567.1"
                     /translation="MKILDRINELANKEKVQPLTVAEKQEQHALRQDYLSMIRGQVLT
                     TFSTIKVVDPIGQDVTPDKVYDLRQQYGYIQN"
     gene            complement(22649..23149)
                     /locus_tag="ACGEXS_000521"
     CDS             complement(22649..23149)
                     /locus_tag="ACGEXS_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011568.1"
                     /translation="MTSIFVFAYLNNTSGNMLFRQQMICACLLANVRHSSIVCLNNIC
                     CIVDMRCLNTKIGGIDVSETDEPQGFERTHNILNINQSSLGVVTYITNKLKSTLKQHI
                     IIARGKKRIDYRLSYNFYTTYYDNVEIKKIDCEYTYAMPIAILIRHTMSFDNAHFFAQ
                     LRHPVL"
     gene            complement(23347..23916)
                     /locus_tag="ACGEXS_000522"
     CDS             complement(23347..23916)
                     /locus_tag="ACGEXS_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8011569.1"
                     /translation="MMESKSIDPRIVRTKQLLVDAFLKISREKKLSQITVKDITDIAT
                     LNRATFYAHFTDKEDLLDYTLSVTILKDLNDNLSISNVINEKVLRNIFISIASYIKDA
                     AKSCELNSEAFCNKAHQRINNELEDIFAIMLENSYPEHQRDIIVNSASFLAAGISGLA
                     LHWFNTSQETADVFIDRNLPFLIHHIAHF"
     gene            24101..24199
                     /locus_tag="ACGEXS_000523"
     CDS             24101..24199
                     /locus_tag="ACGEXS_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011570.1"
                     /translation="MYCNNMFEKLPISLSKNSEFCDILFFIVLSYR"
     gene            24326..25381
                     /locus_tag="ACGEXS_000524"
     CDS             24326..25381
                     /locus_tag="ACGEXS_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFF8011571.1"
                     /translation="MTSIKTITPKDFIFRVLSGVAIGIVAGLVPNAILGEIFKYFMDY
                     HPIFKTLLGVVVAIQFTVPALIGALVAMKFDLSPLAIAVVASAAYVGSGAAQFKNGAW
                     MITGIGDLINTMITAAIAVLFILLIQHRVGSMALIVFPTVVGGISGAIGVLILPYTKM
                     ITTAIGNMVNGFTELQPIVMSILISMVFSLIIISPLSTVAIAFAIGITGLAAGSASIG
                     ISATEAVLIIGTSKVNRLGVPLSVFFGGVKMMIPNMVKYPILMLPILTTAIVSGLVSA
                     LVGIHGTKESAGFGFIGMVGPINAFKFMEVDSAWLSVLLIVVAFFVVPFVTAWLADII
                     YRKVFRLYTNDIFKFMG"
     gene            25989..26690
                     /gene="isaA"
                     /locus_tag="ACGEXS_000525"
     CDS             25989..26690
                     /gene="isaA"
                     /locus_tag="ACGEXS_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase IsaA"
                     /protein_id="HFF8011572.1"
                     /translation="MKKTIMASSLAVALGVTGYAAGTGHQAHAAEVNVDQAHLVDLAH
                     NHQDQLNAAPIKDGAYDIHFVKDGFQYNFTSNGTTWSWSYEAANGQTAGFSNVAGADY
                     TTSYNQGSNVQSVSYNAQSSNSNVEAVSAPTYHNYSTSTTSSSVRLSNGNTAGATGSS
                     AAQIMAQRTGVSASTWAAIIARESNGQVNAYNPSGASGLFQTMPGWGPTNTVDQQINA
                     AVKAYKAQGLGAWGF"
BASE COUNT         8907 a         4171 c         4991 g         8692 t
ORIGIN      
        1 ttcagtatag atatttttac gtgtagtcac gtgtaaaata aattcaatta ggttaggaga
       61 cataattatg aaaacatttg gtaaaaaggt tgtattaatc ggagatggat ctgtaggatc
      121 aagctatgcc tttgcaatgg ttacgcaagg tgttgctgat gaatttgtaa ttattgacat
      181 tgcaaaagac aaagtaaaag cagatgttca agatttaaac catggtacag tccacagtcc
      241 ttcaccagtt gatgtgaaag caggtgaata cgaagactgt aaagatgcag atttagttgt
      301 tattacagct ggtgcacctc aaaagccagg tgaaacacgt ttacaattag ttgaaaaaaa
      361 tactaagatt atgaagagca tcgttaagag tgttatggat agtggctttg atggatattt
      421 cttaatcgcg gcaaaccctg tagacatttt aacaagattt gtaaaagaat atactggatt
      481 accagcagag cgtgttatcg gttcaggtac tgtattggac agtgcacgtt tacaatattt
      541 aattagccaa gaacttggtg ttgcaccttc aagtgttgac gctagtatta ttggcgagca
      601 tggtgatact gaacttgcag tttggtcaca agcaaatgta gcaggtattt cagtatatga
      661 cacattaaaa gaacaaactg gtagcgaagc taaagcggaa gaaatttatg tgaatacacg
      721 tgacgctgct tatgaaatta tccaagctaa agggtcaaca tactatggta ttgcattagc
      781 attgatgcgc atttcaaaag ccattttaaa taatgaaaat aatgtcttaa atgtttctat
      841 acaattagat ggtcaatatg gtggtcacaa aggcgtttac ctaggtgtac caacattagt
      901 taaccaacat ggcgcagtta aaatttatga aatgccatta agtgccgaag aacaagcgtt
      961 gttcgataaa tctgttaaaa cattagaaga tacatttgat tcaattaaat atttattaga
     1021 agactaagcc tattttaagt attaattaga aatatattaa tggtaagagg atctattagt
     1081 gttgcagtaa cacgtggcac tgataggtcc atttttttag caagcattga tagaggcaat
     1141 acttttgcgt aaaataataa ttgaataaag tattatgact cgtgaaaatt tagtgacatg
     1201 aataaaaatg ttgaggcgtc attgagtata aaggaaagta gttctgcatt aatcacgaag
     1261 tagagcatga caacgaggaa taactatagg gagatggttt tggaatgacg aatgtcttgt
     1321 atcaacatgg tacattaggt acgttaatgg ctggcttact agaaggcaca gctacaatta
     1381 atgaattgtt agaacatggg aatttaggga ttgcaacgtt aacagggtct gatggcgaag
     1441 taatattttt agacggaaag gcatatcatg ctaacgagca taaagaattt atagaattaa
     1501 aaggcgatga gaaagtaccg tatgcatcga ttactaattt taaagcgagt aagacatttc
     1561 cattgcaaca attatcacaa gatgatgtat ttgcacaaat taaaaatgaa atgttaagtg
     1621 agaatttatt ttcggctgtt aaaatttatg gcacatttaa acatatgcat gtacgaatga
     1681 tgcctgctca gcaaccgcca tatacacgtt tgattgattc agcacgcaga caacctgagg
     1741 aaaaaagaca agatattcgt ggtgccattg ttggattttt tacaccagaa ttatttcatg
     1801 gcgtagggtc tgctggtttt catatacatt ttgcggatga tgaaagagct tatggtggac
     1861 atgttcttga ctttgaagtg gatgacgttg tcgttgagat acaaaacttt gaaacattcc
     1921 aacaacattt cccggtaaat aacgagacgt ttgttaaagc gaaaatagac tataaagatg
     1981 tggcagaaga aattagagaa gctgaatagt ctaatataat taaaagacct tagcgatatt
     2041 aggaacaggt ggttctaaat gcatcgttaa ggtcttttta tattatatat gtgcttacat
     2101 attttgttga tacgcgcgta aaaagctata aatagtatca agatatgggg tgtcaagatt
     2161 gtgttcacgt gcacgtcgat aaataaagcc ttgaatcgct tcgacttcaa gaggttgctg
     2221 atgcgcaatg tcgtaataca tgctcgttcc catttcgtcg ggataacctt gatatatagt
     2281 cataatagta tcgacagttt gttctgaaaa gtttaacccc tctgcctgtg caacacgaca
     2341 accatctagt aatagctgtc tacacagtgt acgtatttca ggattatgca tgattgcaac
     2401 ggtctgtcta ccgagtgctg tgatagaatt aatgcccaag ttaactaaca atttatacca
     2461 aatagcttgt tgaatatttg cttctaatac aatgtcgatt tgactgtctt ggaccaaatc
     2521 tctaaattgt cgagttaatg cattatcttg tatacgtaat tgataatctc tgaagtgcgt
     2581 aacaacatcg cctttctttt gaccacttat atagacaact gcttggcata cgtttttaaa
     2641 tgaaatatgt tcgagttgac cataaccatt ttgggctaaa atgatgagcg tgtcctcatg
     2701 tgcaagatga gttaaatgag gaataactgc atcaagttga tgtgttttga ctgcaataat
     2761 aataacatca aatgtgtttg tgacatcttc ataacctttc acaacaatat cttgtgcagg
     2821 tgcatgtggt acagtataat atgttattgt tttggcgtgt ctcccgataa gcgttgtatg
     2881 aggcaatgat tgttgtaatt cataggcaat agttgtaccg acagcgcctg ggccgataat
     2941 cgcaactgat aacatcttct tctttccttt ctataaaaga ttcattatac tatttctaat
     3001 aatactgcaa aaagaggttg aataacattg aaaacagtaa gtcaattaat agatatgaaa
     3061 caaaagcaaa ctaaaatttc tatggtaaca gcttatgatt ttccaagtgc taaacaagtg
     3121 gaagcagcgg gtattgatat gattcttgtt ggggattcac ttggtatgac tgtattaggt
     3181 tatgaaagta ccgtacaagt gacgttagca gatatgattc atcacggtcg tgcagtaaga
     3241 agaggcgcac caaatacatt tgtagttgtt gatatgccga ttggtgcagt aggcatctct
     3301 atgacacaag acttaaatca tgctctaaag ctttatcagg agacgaatgc aaatgcaatt
     3361 aaagcagaag gagcgcatat tacaccattt attgaaaaag caacggcaat tggtattcct
     3421 gttgttgcac atttaggatt aacacctcaa agtgttggcg ttatggggta taaattacaa
     3481 ggtgcaacga aagaagcagc ggagcaactt attttagacg cgaaaaatgt agaacaagct
     3541 ggtgcagttg cgttagtact tgaagcaatt ccaaatgatt tagcagaaga aattagcaag
     3601 cacttaacaa ttccagtcat tggtattggt gcaggaaaag gtacagatgg tcaagtattg
     3661 gtttatcacg atatgttaaa ttatggtgtt gaacataaag cgaaatttgt gaagcaattt
     3721 gctgatttta gtgttggtgt ggatggttta aaacaatacg atcaagaagt taaatctggt
     3781 gcatttccat cagaagaata tacgtataaa aagaaaatta tgaatgaggt taataacaat
     3841 gactaagctg attactacgg taaaagagat gcaacacatt gttaaagcag ccaaacgctc
     3901 aggcacaacg attggtttta tcccaacaat gggtgcctta catgatggac atttaacgat
     3961 ggtacgcgaa tcagttagta caaatgatat tacaattgtg agtgtatttg ttaatccatt
     4021 acaatttggt ccaaacgaag atttcgatgc ttatccgaga caaattgata aagatttaga
     4081 attagtatca gaagtaggag cggacattgt ttttcatcct gcagtagaag atatgtatcc
     4141 aggtgaatta ggtattgatg tcaaagtagg cccattagct gatgtgttag aaggagcgaa
     4201 gcgtccaggg cattttgatg gggtggtaac agtagttaat aagctattta atattgttat
     4261 gccggattat gcttattttg gtaaaaaaga tgcccagcaa ttggctattg tcgagcaaat
     4321 ggtaaaagac ttcaatcatg ccgttgaaat tattggtatt gatatcgttc gagaagcaga
     4381 tggtttggcg aaaagttcaa gaaatgttta tttaacggag caagaacgac aagaagcggt
     4441 acatttaagt aaaagtttgc tattagcaca agcgttgtat caagacggtg aacgtcaaag
     4501 taaagtaatt atagacaggg ttactgaata tcttgaatca catataagtg gacgcattga
     4561 agaagttgct gtttatagtt atcctcaatt agtagaacaa cacgaaataa ctggacgcat
     4621 atttatctcg ttagctgtta aattttcaaa agcgcgttta atagataata taataattgg
     4681 agctgaataa agtgataaga acaatgatga atgctaaaat tcatagagca agagtgactg
     4741 agtcaaattt aaattatgta ggtagcatta cgattgattc agatatattg gaagcggtag
     4801 acatcttgcc aaatgaaaaa gtagccatcg tgaataataa taatggtgca cgttttgaaa
     4861 catacgtaat agctggtgaa agaggtagtg gtaaaatttg tttaaacggt gcagcgtcaa
     4921 gacttgttga agtaggcgat gtagtcataa tcatgactta tgcacagttg aatgaagaag
     4981 aaattaaaaa ccatgcacct aaagtagcag taatgaatga agataatgtc attattgaaa
     5041 tgattcatga aaaagaaaat acgatagtgt tataaaatta aatagccatc aataataaaa
     5101 agggccacac cttggtttaa gattccaagc gtgtggcttt ctcttgttat acagtcctat
     5161 cactatgtca cttttaatca aaagcgaccg taattgctgc atatcaatta acgataggaa
     5221 tgattaattg tgaatcatat ttaccaccag tataaatcat gtgcatgcct ttatttacgg
     5281 tttcttcatg ttcataacgt gttttcatgc caggagtact attaccaatt acaatttcac
     5341 taccctttac aacaacttcc aatgtttcgc cttgtttaaa tagcgtgcct gaaggtaaca
     5401 attcgatttc aacaggtaca atctcatctt gtgacaactt taattctgtt tcatgtttat
     5461 gccaaggttg cgcgattgag gatttttctt gatctaattc acgatgtgat acgcgtaacc
     5521 aaccagtagc tacttgacca ttttcaatat gattaaaatc agggaagtta acttcattac
     5581 cacgacgatc taacttttta atacctgcaa ataaatccat atcatctgag tctttagtgc
     5641 ttacccatag ttttaagttc atgtttccaa ctaactcagt atctttgtca aacgtatatt
     5701 taaaacttac atcttgttgt ttatcttcag agtcatattg tgcgacatgc gcgctactaa
     5761 tctttgcatg attcaatgtg tgattttcag cattcaaata caatggtgta tattctgcgt
     5821 taggtaaagg gaacgctgac gctgatttga attcgccttt ataaaattga tctctaactt
     5881 cataaatgac atgaggcgta tctttccaat cgttattttc ttctttaagg taaaaatcaa
     5941 agaatgattt ttggcgttcg agattttctc tagcgtagta actttcccac tctttacgtc
     6001 catgcacata tagccatttt tcttcagatg cagcttgttt aaatccttca aaagagccac
     6061 ggttgtgcaa accttgtgta gaccaactag cacatgttag tagaggtgtt ttaatttgtg
     6121 ataatggcac ttgacgctgt ttccaaaaat catcgaacag aggatgttct tgttgtgctt
     6181 gaatcaaatc ttcgatattt ggattatctg tccatctcgc aaaaatacct tgagtccaga
     6241 aacgataaaa gccagtatct ggtatacctc cgtgaaaggc tacttctcta tacatatcat
     6301 ttaagccttc ccaaggaatc attgctttta aatgtggtgg atttaatgat gcgacccacc
     6361 attgagtcac cgcaagataa gaaacaccat ttgtcccgat atttccatta ctccatgact
     6421 gatttgctgc ccattcaatc acttcgtaat aatcttccgc ttctcttttt gaccatggag
     6481 ataagacgcc tttggatttg tcactaccgc gtaatgcaac tttaactaca acataatcat
     6541 ttggcaccca aaatcctggg tctggtgatt cttcaggtgt aaaactagat gtcggaatgg
     6601 tacctaatgt tggccaaagg gcacccatat ttgtgatttt aggcttatta tctttaccgt
     6661 aagtatctgc agacataact acagggaatt tgccatcttt atttggtctg aaaatattaa
     6721 tatataattt ttcgccatcg cgcatttcca cagtgacatc tttttccata atcatttctt
     6781 gatttccata ttgaacactg tcgacaacga tgtggttaat accggcttta acttcattga
     6841 catgagttac agttaatttt ggatttccta gtaaatgttg gttcattgtt atcacctttt
     6901 tctgtttgtt tgtatcacct atattaaaca ggtaaacatt gtgtaccaat catcaatgtt
     6961 ggagatttgt gggataatga acataacgtt tataatcatt ggggaggact tgatataaat
     7021 gactatagat cgacgtatta gaaaatcaca agttgcaatg agaaatgcat ttattgaact
     7081 tttacatcaa caccaattag aagagattac tgtgcaacaa attgcagatt tagctgatgt
     7141 taaccgtagt acattttata cgcattatta tgataagtat gatttgcttg aaaaattaga
     7201 ggaccagcag ttggaggaaa taagagactt cattcatgat gagaagttaa atggagcagt
     7261 taagttatcc acggataaca tacatcaaat tatgaccgtt ttaatagaaa tgatagaaaa
     7321 agacatcgca ttttatcaat tgatgtttcg tatgggtaaa gattcaaata tccacgaaaa
     7381 actttatgat ttaattatgt gtcatttaca acgttataaa aatgaaaatg atcaaattaa
     7441 tggtatacca ttttcttatt tcatgagtta tgtttcaggt gcgggattgt cttttataag
     7501 acattgggta gaagatccaa accgtataga caaagttgac ttaataaggc attttaatac
     7561 gattgtcaat caagggccag caacgataat taaaaatata caataaaata aggcaagcca
     7621 tgaatcgaga gtggcttgcc ttgctaaata acataagctt attaaatgat atctttatca
     7681 ataacattat tgttggattg cagtatagtc ttgaccatgt aacatccaac gaacaccgta
     7741 tttatcggta aagacgccca ttttgccacc ccagaattgg tcagcaaacg gtaattctat
     7801 ttcaattgaa gaatgatctt taatttgctc atagaatgct tcaactttat cagcatcttc
     7861 cttattgtta acatcataat caattaataa tgatatgcca ttattaattt tgtcagcgcg
     7921 accaaaagaa tcagaacata acacttttac gccaagcact tcaaattcag catgcatagt
     7981 tgcttcttgc gcttcttcct tagtcatacc caaatgtgac gcttgttctt cgccaacttc
     8041 taaacgttta acgtcagttg caccaaatac ttcttcgtaa tatgcaaggg cttcttttga
     8101 attttcaaaa gcgatataag ggaataatgc tgtcatgtaa aacacctcaa tgtattattt
     8161 ttattactaa caccatgtta cattaaaaaa tggcaaaagt ataactttta cgatgaattg
     8221 aagtcaatct tttgtaaaat acacgataaa ttataagatg aaattgattt aaatgaagta
     8281 aacatatata tgacatacaa aaaatgaaaa tgacacagag acgtatgtgt tgtttagtgt
     8341 tttaaaattc aagtttaaaa aatgctttta gatacataag ggattttcga aatataaatg
     8401 cattaaaatg aatttctaac ttttttgact gacatttaat atgaatcata tataatagaa
     8461 gttaatgatt aaatgtggga ggttaaatca tgattaatgc tgaacctatt atcagtaaaa
     8521 tgaaaaatca aaaaattaat tatgacaagg tgttgaagaa attaatcggg cagtgggaaa
     8581 gagaagcaat cagacctaaa atcttattgc atagttgttg tgcaccttgt agtacatata
     8641 cattagagtt tttaacacaa tatgcagaca ttgcaattta tttcgcgaat tcaaatattc
     8701 atccgaaaaa tgagtactta cgacgtgcta aagtgcaaga acaatttgtg gaagatttta
     8761 atcgcaagac tggtgcgaat gtgaagtata ttgaagcacc ttatgaaccg cataagtttg
     8821 tgaaaatggt gaaggataaa gaattagctg atgaaaaaga aggcggctta cgttgtaccg
     8881 cttgttttga aatgcgtttg gatattgtag cgaaagcagc tgtagaacat ggctatgatt
     8941 attttggcag tgcaatcaca ttatcaccta aaaagaacgc acaattaatc aatgaacttg
     9001 gtatggatgt ccaaaaaata tacgatgtga actatttgcc aagtgatttt aagaaaagta
     9061 aaggttatga gcgttccata gaaatgtgta atgactataa tatctttagg cagtgttatt
     9121 gtggatgtgt ctttgcagcg atgcaacaag gtattgattt taaaacagtt aataaagaag
     9181 cgaaagcatt tttagaacaa tatccagatt aatcattgga aatgagataa aaggcaagaa
     9241 cgtatacatg ccatatccca ttaaaaatta tcaaatagaa acccgagatt ttagtgaaat
     9301 gaatcatact aaaatctcgg gttatttata taatcgtaaa tattgtagat ataatgaata
     9361 actggatcct gagacataaa acaatgtctc actctaatta catcatttta tgaacaaatc
     9421 ttataattgc taaaatgaca atgacaagtg ttgaaataag tgaaaacact ttaaactttt
     9481 tatagtcatt tttcgtttca ttttctcctg ttttcatcat ttcacgtagc tcttgctcga
     9541 tttctaaatc aagatctgtt ttacgataac cattcaaacc ttgacgttca tcttcttcag
     9601 gttgattttg ttgctgtgtg tctttgttgt cagaagtcgc tactgttttt ttattatctg
     9661 tttctttagt cataacaaac gcctccgtta taaaacgcta tatttaatga tatgtgatat
     9721 ttaagccatt attttgaaaa atggtgataa atatatcttc ttgagttata tcataatttt
     9781 aataagacga ttcagcaagt tttaaagtat tatttgacta tgttggatta ggcatctagt
     9841 cctataatat cactgacatt gtcaaaatga tgatctttta agtaacgtgc gatgcctttg
     9901 ttcattttct tagttaaacc tgggccttca ataacaagtg atgaataaat ttgaataagt
     9961 gacgcaccgt gacgcatcat tttgattgca tcttcagtac tgaatacgcc gcctgtacct
    10021 ataattaaaa attcaccatt tgtttgctga taagcatact taatcaattt taaattacgt
    10081 tcaaataatg gacgaccact caaaccgcct tcttcgactt tattagcaga agttaaacca
    10141 tctcgttgtc gcgttgtgtt tgctaagatg ataccgtcaa atgtctcagt aatcgctggt
    10201 aatagtgctt ttaagccatc gaaatccata tcagacgtta gttttaaata aattggcact
    10261 gttacatcat gttgtttttt aaatgctgtt aaagcttggc ataacattga aaattcatct
    10321 ttatcatgga agttttgaag attttcagta tttggagaac tgatgttgac tgtgaaaaat
    10381 gaaacgtcgt gtttaaacgt atcaataacc tttatataat cttgataacg cgcttcataa
    10441 ggtgtcattt tattcacacc aacattgata ccaacaggta cttgataagc atttttacgc
    10501 aaatgactta gtgctttgtt cataccaata ttattgaagc ccattcgatt tatcaaggcg
    10561 tcatcttcta ataatctaaa catgcgtggt tgagggttac ccggttgagg tttaggtgtg
    10621 ataccaccta attctaaagc accgaatcca aggtgttcca atgcttttgg tacttcgcaa
    10681 gatttgtcga aaccagctgc taagccaatt ggattgtcgt acgtattacc ttgtatcgtt
    10741 tgtgataacg ttggattctt ataagtaaat agtttatcga cgactgggaa taaaaccgga
    10801 aacttttgta acgtttttaa tgcatcgata gttagtccgt gtgctttttc gggttcgatt
    10861 ttgaataaga aaggtttaat taatttgtac atgagtatgc tcctatttca ttatatttga
    10921 ggcttactat cctcaactta atatatgtga aatatattct tttaatagac tagcatttcc
    10981 atacataatt tcctagttaa aactaaaaag ttttgaaaat tgacgcaagt ttgaataacg
    11041 tttttaagat taaatcatcc taattaggca atattatagt ataaagtaag tagattggaa
    11101 ggtgtttgta tgaatgaaca atggttagag catttacctt taaaagatat taaagagatt
    11161 tcaccagtga gtggtggtga tgtaaacgaa gcatatcgag tcgaaacaga tacggataca
    11221 tttttcttac ttgtccaacg tggacgtaaa gaatcatttt atgctgcaga aattgcaggt
    11281 ttaaatgaat ttgaacgtgc aggtatcacg gcacctagag taattgcaag tggcgaggtt
    11341 aacggtgatg cgtatttagt gatgacgtat ttagaagaag gggcttcagg gagtcaacgc
    11401 caattagggc aactcgtagc tcaattacac agtcagcaac aagaagaagg caaatttggc
    11461 ttctcattac cttatgaagg tggcgatatt tcttttgata atcattggca agacgattgg
    11521 tgtaccattt ttgtcgacaa acgcttggac catttgaaag atgagttgct aaatagaggg
    11581 ctatgggatg ccaacgatat caaagtatat gacaaagtgc gacgtcaaat tgtggcggaa
    11641 ttagaaaagc atcaaagtaa accgtcttta ttacatggtg acctatgggg tggtaattat
    11701 atgttcttac aagatggtcg tccggcgtta tttgatccag cgccattata tggtgacaga
    11761 gaattcgata tcggtattac aacggtattt ggtggtttta cgagcgaatt ttatgatgcg
    11821 tataataaac attatccact cgcaaaaagt gcatcctata gacttgaatt ttatcgttta
    11881 tatttattga tggtccattt attgaaattt ggtgagatgt accgtgatag tgttgcgcat
    11941 tctatggata agattttaca agatacaaca agttagttaa gacgttagat tgagataaat
    12001 agataatatg cacagatatt tttacaatga gaagcgatac agctgcctca ataaaaatat
    12061 ttgtgcgttt ttattgttgg aaaataaaat tttaatcgct attgttaatt tctgtaatgt
    12121 aaaacaaggt tgagttacaa taaaagtgat tttataactt tttgttcaat aaaattctag
    12181 gaatgataca tatttattga tacaataatt ttgaatataa tcataaaaca atatttaagt
    12241 ataattgaat gtttgaatat catatattga tacagtttct aataatttta aaataattta
    12301 aatggagaga ggtgtaaatg atgagtacag ttcaaagtga tatttttaag accaatagtg
    12361 catcatcatc tattaaaagc gctgttgaaa catgtaataa tgtgtcgaaa ccggataaag
    12421 atgaaagtac aacagtaagt ggaaataata atgctcatag tgtgatagat gatttgatga
    12481 gtaagaatca atctgttgct gaagcaatac gaactgcgag cgataatata caaaaagttg
    12541 gtgaggcttt tgaccaaact gacgtaatga ttggtaatga aattggtaaa aattaaaacg
    12601 tggtgaaatg atgtcgaata aactggatga aatcaataaa ataatcacag cgaaacatga
    12661 gcaaatggat gacttatatg atgaaaagcg agaggttaaa gcattgatag atgaaagtga
    12721 tgcgcttaat cattcgatag atcaattata tcaacattta ggtgagcgtt attatagtag
    12781 caatatggct agtcgtatgg aacagttccg cgatgaattt cattttgcga aacgacgttc
    12841 aacggaagcg ttatacgagc agcaacagca aattcaacat ggcattcgta aagtggaaga
    12901 agagatgatt gacttggaaa tgcgaaggaa tgttgaaatt gagacggtga caaaggagga
    12961 aaataaatgg aaacaatagg aagcattatt tatttaaaag aaggttcgca aaagttaatg
    13021 attattaata gaggaccaat tgtagaaatt gaaaatcaaa agtatatgtt tgactattct
    13081 gcatgtaaat atccgattgg tgttgtagaa gatgaaattt attattttaa cgaggaaaat
    13141 atagattcag ttatttttaa aggttattct gatcaagatg aggttagatt tcaagagttg
    13201 tttgaaaata tgaaacaaaa tttggatagt gaaatacaac gtggagaagt tacacaacaa
    13261 taaagaaata ctttttcttt attggggtgg gacgacgaaa taaattttgt aaaaatatga
    13321 tttctgtctc accgccaaaa tgttatctcc aagtactgag gctggatgat accatgaaat
    13381 gtcattcata atcatttgaa ctaaacgtag cagccttaaa ttttaaaaaa agacacatac
    13441 caacttccga aatgtagatg aattctctac aataacggaa gtttttcttt taatattgaa
    13501 atttctcaag gataggtcta tactttataa atcgtaatta ttacgattta taatcaaaaa
    13561 caataacttg aaatagatca ttgagggagt gttaatatgc aacatcataa agtggctatt
    13621 atcggtgccg gtgctgcagg tataggtatg gccattacct taaaagattt cggtataaca
    13681 gatgtcatta ttttagaaaa aggaacagta ggacattcat ttaaacattg gccgaaatcg
    13741 acccgtacga tcacgccatc atttacgtct aatggatttg gcatgcctga tatgaatgca
    13801 atttccatgg atacttcacc agcatttaca tttaatgaag aacatatttc cggagaaaca
    13861 tatgctgaat atttacaagt ggttgccaac cattacgagc tgaatatctt tgaaaataca
    13921 gttgtcacaa atatatctgt agatgatgca tattatacga ttgcaacgac aacagagata
    13981 tatcacgcgg attatatctt tgtcgcaaca ggtgattata atttccctaa aaagccattt
    14041 aaatatggta ttcattatag tgaaattgaa gactttgata actttaataa ggggcaatat
    14101 gtggttatcg gaggtaatga aagtggcttt gatgctgcat atcaacttgc aaaaaatggc
    14161 tctgacatcg cactttatac tagcacaacc ggtttaaatg atccggatgc tgatcctagt
    14221 gttagattgt caccttatac acgtcagcga ctaggtaatg tcattaagca aggtgctcgc
    14281 atcgaaatga atgtacatta tacagttaaa gatattgatt ttaacaatgg acagtatcat
    14341 atcagttttg atagcggaca aagtgtgctt acacctcatg aaccaatact agcaactggc
    14401 tttgatgcaa caaaaaatcc aatcgttcaa caattatttg tgacaacaaa tcaagatatt
    14461 aaattaacaa cacatgatga atcgacacgt tatccgaata tttttatgat tggtgcaaca
    14521 gttgaaaatg ataatgccaa attatgctat atctataaat ttagagcgcg atttgcagta
    14581 cttgcacatc ttttaacaca gcgggaaggc ttaccagcta aacaagatgt cattgaaaat
    14641 tatcaaaaaa atcaaatgta tttagatgat tattcatgtt gtgaagtgtc atgcacatgt
    14701 tagaagtgaa atatgatatg agaactgggc attatacgcc catacctaat gaacctcatt
    14761 atttggttat tagtcatgcg gataaactta ccgcaacaga aaaagcgaaa ttaagattat
    14821 taatcataaa acagaaatta gatatttcat tggcagaaag tgtagtttct tcgcctatag
    14881 cgagtgaaca tgtgatagaa caattgacac tatttcaaca tgagcgacga catttaagac
    14941 ctaaaataag tgcgacattt ttagcctggt tgttgatatt tttaatgttt gcattgccaa
    15001 tcggtatcgc ttatcaattt tcagattggt ttcaaaatca gtatgtgtca gcatggatag
    15061 aatatttaac tcaaacaaca ttgctcaatc acgatatatt acagcatata ttatttggtg
    15121 attatggtgt gctatcactt ggaacatatt cgctcgtatg ggcattgccg gttgtaatat
    15181 tgattagttt atcaactgct ataattgatc aaacaggact caaatcatgg atgatatggg
    15241 caattgaacc gtcaatgtta tggataggat tacaaggtaa tgatatcgtg ccactattag
    15301 aagggtttgg atgtaatgca gcagctattt cacaagcagc acaccaatgc catacctgca
    15361 cgaagacaca gtgtatgagt ttaataagct ttggtagttc ttgtagttat caaataggtg
    15421 cgacattatc tatttttagt gtagctggaa agtcatggct atttatgccg tacttaatat
    15481 tagtactttt aggtggcatc ttacataata ggatatggtt gaaaaagaat gatcaacaac
    15541 ttagcgttcc gctaccttat gataggcaat tacatatgcc aaatatacgt caaatgttgc
    15601 tacaaatgtg gcaaaatata caaatgttta tcgttcaagc gctacctatt tttatcacaa
    15661 tctgtcttat tgttagtatt ttatcactaa cgccaatttt gaatgtttta tcacaaatat
    15721 ttacacctat attatcgtta ttaggcatct cgtcagaatt gtcaccaggg attttatttt
    15781 caatgattcg aaaagacggc atgctcttgt ttaatttgca tcagggcgcc ttattacaag
    15841 gaatgacagc aacacagtta ctactacttg tgttttttag ttcaacattt acagcgtgct
    15901 cggtcacaat gacgatgctt ttgaaacatt taggtggtca gtcagcacta aaattaattg
    15961 gaaagcaaat ggtgacatca ttgtctttag ttattggtgt aggcatcatt gttaaaatag
    16021 taatgctgat tatttaaaaa aaatgaacta taactgaata tagagtcatg tcagtcaata
    16081 ggagatctat cttggaatat gctattcata tgaagtataa gaggagagtc gcagatgaaa
    16141 atagttatta taggtgggtt tttaggtggc ggtaaaacga ctgtcttaaa tcatttgctc
    16201 gctgaatcat taaaggaatc gctgaaacca gcagtcatca tgaatgaatt tgggaaaatg
    16261 agtgttgatg gtgccttagt atctgaagac atacctttaa gtgaactgac agaggggtgt
    16321 atctgttgtg caatgaaagc agatgtatca gaacagttac atcaattata tttaaaagag
    16381 caaccagaca ttgtatttat tgaatgtagt gggattgcag aaccggtctc tgtcttagat
    16441 gcttgtttaa cgcctatttt agctccgttt acaacaatta cacatatgat tggtgtaata
    16501 gacgcaagca tgtataaaca cattaaatca ttccctaaag acatccaagg cttattttat
    16561 gagcaattag catattgttc tgtcttattt gttaataaaa tagattcagc agatgttgaa
    16621 acaacgagca aactattgaa agatttagaa gttattaacc cagaggccga tatacaagtc
    16681 ggtatgcatg gcagcgtcac tttgccaata tcagttagac aaatgacagc aacttctgac
    16741 aataaacata agtctttaca tcaaatgatt aatcatcaat ttgtgcaatc accagtcaaa
    16801 tgtactaaag cagagtttat aaaacgttta gcatgccttc cgtctcatat ttataggttg
    16861 aaagggttta tgacatttga agacaccgca catacgtatc tcattcaatt tacacaagga
    16921 caatatgaat taacacctgt agcattttca aaaaaagtgc cagaatattt ggtgcttatc
    16981 ggaaagggta tttcaaaaga agactatcaa tgtttggaac agtagtgttt tcagtggaag
    17041 agaatggtta acatgccttc atgtataata acgagttgat ttgaacgttt aagcgtaaat
    17101 aaaaataagc ttggtcagcc atcaaatata atttgaaaac tgtccaagct gttttattag
    17161 agaacaatca attaacccca catatttaat aatacatcag caaagccttc aggtttttga
    17221 atataaccta agtgaccgcc tggaatatct acaataggta tgccagtttc tttatttata
    17281 taaaagttaa catcttgtgg gaaggagcct ctagaatctg tcccatttag tagggtgatt
    17341 ttatcgctgt attttgtgaa atcatccaaa gtaatatctg aatgcgtata ttgtctaatt
    17401 tcaaattctg accagaacat cgtacgtttg tactgttcta tacgtccttc ttcagtatca
    17461 gcaggttgag acatcatttt tgcatcaatt ggtgcgatat ttaatgtttc gccaaatgtt
    17521 ttcatgcctt tttctaagcc ttctgttaaa atttgatgca caatgtcatc atttttatct
    17581 ttccaataag tactgtctgg taaaaatgta ttaattggtg gttcgtgaaa tgcaatcttt
    17641 ttaacgactt cagggtaatc ttttaacaca tgcatcgcaa cgattgaacc tgaacttgaa
    17701 cctaatatat agacaggttc atcacttaat gactttgcaa gttcggcaat gtcctgtgcg
    17761 tcgcgtttga cacgataatc actgtcaggg tttgaagcgg aatcagggag tggttcagtt
    17821 aactcgcttt ctccataatc acgacgatca acggctacaa cagtaaaatg gtcttttaac
    17881 tgttctgcaa gaggcagaaa aatgtctccg gtaccgtttg caccaggaat aaagatgagc
    17941 acgggtcctt gtccgacttg gtggtatcgt aatttagcgc cttgtaattc taaagtttcc
    18001 atattcaatg acctccattt gttaattgtt aggtgataaa cctaataatt tagcaccatt
    18061 tgtataactt attttctctt tttcttcatc tgttaaaccc agttcatcta aaaatacacc
    18121 taatttttca ggctcaatat atggataatc agcagcataa agaattctat caatacctac
    18181 ttctttcttg actaaatcaa actgtggctt cgttaacatg ccactcggtg tgatataaaa
    18241 attattttta aagtaatagc ttacagggtg gttcaaatgt tcagcgaata aagcttcatc
    18301 catacgttct aagaagaatg ggataaactc accccaatgt ccaataatca tatttaactt
    18361 tggataacga tcaaaaatac cagataatac tagatgtatt gcatgaatgc cgacatcaat
    18421 gtgccaacca taaccaaaac aagcaaatgt tgccgcagtt acttcaggat aatttccttt
    18481 atagtatgat tgataaatgt cactgttaac tggcgcggga tgtagataaa tcggtacgtc
    18541 taaattttca gctgttttga aaataatgtc atatttgtct tgatcaagaa aaccatcttg
    18601 tgcacgtccc ataatgagcg cacctttgaa tcctaaatca ttgatgcaac gttcgaattc
    18661 tcgcgctgcg gcttcaggct cattgatagg taaagttgca aagcctacaa agcgattggg
    18721 atattgtgca atatagtttg ccaattgatc atttgctttt tgacataatt caatggcttt
    18781 ctgaccaacc aaatttgaag gagaaccatt tccataagat aagacttgaa tttgaacgtc
    18841 ttgattattc ataaattgga tacgttcatc atgatgtgat aattcgtcgg catttgtaaa
    18901 acctgtcttt ttttcaaggc cttctaacat tactttcatc ggtacacctt taggatctgc
    18961 tgatatcgca ttcatcgttt ctttttgaat atcttcaatg acataatgtt cttcaaacgt
    19021 aatacttttc atttacttcg cctccatatt gtattgcatg tttattgcat ctattgcaga
    19081 agcatttttt atatacctct aatttcaatg tttgtaacat aaaattgatc taccaaggca
    19141 tctctccatc gccattaata aatgtacctg ttgggccatc tgcaccaatc gttgctaatt
    19201 gaatgattgg cttgattcct tcagaaacgt gtttggaatt attactaaaa tcaccaacta
    19261 aatcagtatt tgtagcgcct ggatcagcag cattgatttg catgttaggt aatcctttag
    19321 cgtattgtag cgttagcatt gttactgccg atttagacga acaataagct aatgaattca
    19381 ctttagattc agctgtttcg gggtttgtaa ccattccaaa tgaacctaaa ccacttgata
    19441 cgttgacgac aacaggttgt tcagattttt ctaagagagg gacgaatgta ttcatcattc
    19501 gtacgatacc gaatacattc gtttgatata cttcttcaac gtcacgaggt gtcaatttgg
    19561 aaggtgctga aaattgacca gatatacctg cattgttaat gaggatatca agacggcctt
    19621 ctttttcagc aatcatgtta taagcatttt tgactgagta gtcacttgta acatctaatt
    19681 gtacataatg aacacctaat ttttgtgatg cttgttgtcc tcttacatca ttccgagaac
    19741 ctatataaac tttgtaaccc aatgctttaa gtgcctctgc acttgcatag cctaaccctt
    19801 tattgcctcc tgtgattaac acaattttag tcattacgtc ccacctcatc taaataaatg
    19861 tttaataaat aatttctgta cgcttcaatt gaaatatggc gatgctctat ttggaaggca
    19921 aatacactag ttgataatga ttgcaacagc atatctgttt tgaacttcgt gtaagtgtcg
    19981 tcatcgcttt taaataagtc ataataaaaa tcaaataatt cttgataaaa tgcgctttgg
    20041 taaaaacgta atttattgtt gcctgcttca atacattgca gtagtgcctt attatcgatt
    20101 ttaaattgta aaagataatc taacgacact tgcataacct cataattaga atgatagtca
    20161 tctttaattt gcttaaaatg agtgataaaa atatcaaggt ctctttgtat gacgtagtag
    20221 cataaatcgc ttttatcttt gaaatgtcga tacaatgtcc ccataccgat acctagttct
    20281 ttagcaatac gattcatact aatgttttca acgccttctt catcaaaaag tttgtgcgct
    20341 atttcttcaa ttcgttgcct attctctttt gcatcttttc gcatgattac acctacttaa
    20401 aattctctaa aattgacaaa cggataactc tccgtttatt ataaaacgtg ttaagaaagt
    20461 tagcaatgaa tttgcaataa ctattaaata tcataaaaga aaagagtgtt gataatgtct
    20521 ttaaataaag agcaaagacg catcacagct gaagagttgc aagcacattt cgaagcatct
    20581 accttatcgg ttcaaatgat tgctgaaaaa ctgaatgtca ctacagaaga tgtggaaaaa
    20641 gtattagcta tgacagcgcc actaggcatt tttagtcatc aattacaacg atttattcat
    20701 ttagtatggg atgtcagaga tgtaataaac gacaatatta aaggaaatgg acaaacacca
    20761 gaaccatata cgtatttaaa aggtgaaaaa gaggactatt ggtttttaag ataaaaagag
    20821 gagtggatct aaaatgaaag atattttagt aataggcgca acagggaagc aaggtaatgc
    20881 agttgtaaaa cagttgttag aggatggatg gtatgtaagt gcattaacta gaaataagaa
    20941 taatcgtaaa ctttcagaca taggacatcc gcatttatca atcgttgaag gtgatctgag
    21001 tgataatgtt agtttgcaat cagcgatgaa aggtaagtat ggcttataca gtattcagcc
    21061 aatcgttaaa gatgatgtta gcgaagaatt aagacaaggt atgaagataa ttgaaatagc
    21121 tgagcaagaa aatattcaac atattgtgta tagtactgcg ggaggggtta atcgaaatcg
    21181 aactggccca cactttgaag tactagcaaa aatagaaaat agattaatgg aatctaatat
    21241 aaatgcaaca gttatcaaac catcattctt tatggataat tttttacgca ttgctaaagt
    21301 cgaagatgaa cgtattacat taccagaatt tattaatccg aatataaaat tcacaatgat
    21361 ttcttctatt gatatagcta aaattgcatc gtatattttt gcacatccac aaagctttac
    21421 gcatcaatca atagagattg gttccgatga agttacatta agtgaagcag caacaatttt
    21481 tagtgaagtg acaggtaaat ctactgttat agaaggagaa tttgttagtg gtgttgcaga
    21541 aaagcaatgg ttggaagaaa aaggttatga agtagacttt gaattaatgg ctgaaataaa
    21601 tccaacaaga ttatcattaa gtgattggct taaagtccaa aactataata agtaatgaat
    21661 ggggtgcaat catgattcaa tcaatgtggt ttaatttgca tgtgcaagat ttagaaaaga
    21721 gcgcacagtt ttataaagcg ttaggattta aaataaatag aaacccacaa atgttagata
    21781 aaatggtcgg cattcaaata ggtcaaacaa ccgtaatttt aatagaaaac aagcattttc
    21841 aaaatgtaag tcagcaaagc cttaatactg aaccaaatga agtgatgatt tctctaggtg
    21901 tgaacacaaa tgaagaagtt gaccagttgg tgaataaagt gaaagaagcg ggtggcacag
    21961 tcgttcaaga accaacagta agccaagggt tttatggagc aatgttcaaa gatcttgatg
    22021 gacaccattt taattttttg gtctgctaaa atgaaattct caaaagaata aataaaaagc
    22081 ccatacagag atgaagcgta atgtgcacca tggtgcgaca cattagtgtc ttcaatcagt
    22141 atgggctttg tttatgaggt cagttggcac cttaagtgca atacctcgtg agcaaatatt
    22201 aattttgaat ataaccgtat tgttggcgaa gatcataaac tttatctggt gtgacatcct
    22261 gaccgattgg atcaaccact tttattgtgg aaaatgttgt taatacttgt cctcggatca
    22321 tgcttaagta gtcttgacgc aatgcatgtt gttcttgttt ttcagctaca gtaagtggtt
    22381 gtactttttc tttatttgca agttcattaa ttctatctaa aattttcata ttgtcatatc
    22441 ctccttagaa tcaagtgttg atatattttg agattatttt tatatggtaa tttatcaaca
    22501 cattaagtaa tgtttttagt ttctcggtct tttgaccagc agatttttgg aattaaaata
    22561 agggatttga gacacgattt tactagtagt acgatggcaa agtgatgtta ttgaagtgtc
    22621 gtttgatata catgtctgca atgttgtttt ataagacagg atgacgtaac tgagcaaaga
    22681 aatgagcatt gtcgaatgac atcgtgtgtc ttattaaaat tgcaatgggc atagcatagg
    22741 tatattcaca gtcgattttc ttgatttcta cattatcata atacgtagtg taaaagttat
    22801 acgacagtcg atagtcgatt cgctttttac cacgagcaat tattatgtgt tgcttcaacg
    22861 tcgactttaa tttatttgta atgtatgtca ctacacccag actactctga ttaatattta
    22921 atatattatg cgtgcgttca aacccctgag gttcatctgt ctcgctcacg tcaatccctc
    22981 ctatcttagt atttaaacaa cgcatatcaa ctatgcaaca tatgttgttt agacatacta
    23041 tactactatg tcgtacgttt gccaataaac acgcacatat catttgttgc ctaaacaaca
    23101 tattgcctga agtgttgttt aagtacgcaa aaacaaaaat gcttgtcact aaaatctata
    23161 tgttttaatg acaagcttat tttcattcag ttaacgactg ctaatatgac attgtagagt
    23221 ataggacgtt gatttatgtc ctagacacgg ctaatcaatc gtaatacgtt aaattttatt
    23281 gtgtgtacaa gtatgaacct aagtcacata gtgatttcaa gatgcatgac taaataccaa
    23341 gttttattaa aaatgtgcta tatgatgaat taaaaatgga aggttgcgat cgataaacac
    23401 atcggctgtc tcttgactcg tgttaaacca atgtaatgct aagcctgaga taccagctgc
    23461 taaaaaactc gcactattta caatgatatc tcgttgatgc tccggatagc tgttttctaa
    23521 cataatcgca aaaatatctt ctaattcatt attaatacgt tgatgtgctt tgttgcaaaa
    23581 tgcttcacta tttaattcgc aagactttgc agcatctttg atataactcg caattgaaat
    23641 gaaaatatta cgcagaacct tttcattaat gacattagaa atgctcaaat tatcattcaa
    23701 gtcttttaaa atggttacag ataatgtgta gtctaggagg tcttctttat cagtgaaatg
    23761 agcgtaaaat gttgcacgat ttagcgtagc aatatcagtg atatctttaa ctgtaatttg
    23821 agataatttc ttttctctag aaattttaag aaaagcatcg acaagcaatt gtttggttct
    23881 aacgattctt ggatctatgg atttactttc catcatttgt tcactaccta acatctgttg
    23941 ttttaacact tattatcagt atcttctatt atattgtcaa ttgtgttgat gatatgtaag
    24001 tgctgatacg ctcctaacaa ataatgtacc tttttggtaa gcatttgaat gtctttcttc
    24061 tattgtatta atggatttta aaaatgaaga taaaataaaa gtgtattgta acaatatgtt
    24121 tgaaaaattg ccaatttctt tatctaaaaa ttcagaattt tgtgatatac tatttttcat
    24181 agtcttgagt tatagatgat actgctagcc taaatataac aacatcggca tgcacatctt
    24241 agaatttaag ctagtggcag cttataactt aaggctcttt attatttgca tacagatgta
    24301 tatacataaa tggaaggatg atattatgac atcaattaaa acaattacac ccaaagattt
    24361 tatatttaga gttttatcag gtgtcgccat aggtattgtt gcgggtttag ttcctaatgc
    24421 gatacttgga gaaattttta aatactttat ggattatcat ccgattttta aaacgttatt
    24481 aggcgttgtc gttgcaattc aatttacagt acctgctttg attggtgcat tggttgccat
    24541 gaaatttgac ctatcgccat tagcaattgc tgttgtggca agtgcagcat atgtaggaag
    24601 cggtgcagcg cagtttaaaa atggtgcttg gatgattacg ggtattggtg atttaattaa
    24661 tacaatgatt acagcagcga ttgcagtgtt attcatttta cttattcaac atcgcgtagg
    24721 tagtatggca ttgattgtat tcccaacagt tgttggcggt atttcaggtg caattggtgt
    24781 tttaatattg ccgtatacga aaatgattac aacagccatc ggtaatatgg ttaatggttt
    24841 tacagaatta caaccgattg tcatgagtat tttgatttca atggtattta gtcttattat
    24901 tatttcgcca ctatcaactg tggccattgc ctttgcgata ggtattacag gtttagcagc
    24961 tggatcagcg tcaattggta tctccgcgac tgaagcagta ttgataattg gtacaagtaa
    25021 agtgaatcgt ttaggggtgc cgttatccgt cttctttgga ggcgttaaaa tgatgatacc
    25081 taatatggtg aaatatccaa ttcttatgtt accaatatta acgacagcca ttgtatcagg
    25141 tttggtatca gcattagtag gtattcatgg aacgaaagag tcagctggat ttggttttat
    25201 aggaatggta ggaccgatta atgcatttaa atttatggag gttgattctg cttggttgag
    25261 tgtcttacta attgtagtag cgttctttgt tgtaccattt gtaacagctt ggcttgcaga
    25321 tatcatttat cgtaaagtgt ttcgtttata tactaatgat atttttaaat tcatgggata
    25381 ataacagtat gttttttgaa aatatgagac caaaaattat ttaatgttat tgtgaattgc
    25441 gagaatgaag ttgaacaata taattgagct gcagtttatt tgcgctaata aaagataaga
    25501 caattcacaa aagatgtgtg ttttaaaact ttgaatatac aacaataata gcaatcaata
    25561 catgtgtaga aacatatttg ttgattgctt tttaattgcg tttaatgtac gaaaataaga
    25621 gaagttacat gtcagatata ttacagctat gtaacaaaaa tacaatctgt aatattacga
    25681 aagctgtaat agttgagcta aatatagtaa aaatcttatg tgttaaagac tatttcaagg
    25741 gttgattgga ttctaaagga cacatatttc acattacagt ataaaacagc catatttcaa
    25801 cattacaaat aacacttgat attgtaatgt tttgtaaaga aagtgtaatt tactggctgg
    25861 ttttttgtga tatagtgagt accgttgaaa gaaataaaag acaatttaaa cttgcactac
    25921 gacattcaaa ttcaaaatat aattactaaa aacataaact tacaattatt actggaggat
    25981 tttttactat gaaaaagaca attatggcat catcattagc agtggcatta ggtgtaacag
    26041 gttacgcagc aggtacagga catcaagcac acgctgctga agtaaacgtt gatcaagcac
    26101 acttagttga cttagcgcat aatcaccaag atcaattaaa tgcagctcca atcaaagatg
    26161 gtgcatatga catccacttt gtaaaagatg gtttccaata taacttcact tcaaatggta
    26221 ctacatggtc atggagctat gaagcagcta atggtcaaac tgctggtttc tcaaacgttg
    26281 caggtgcaga ctacactact tcatacaacc aaggttcaaa tgtacaatca gtaagctaca
    26341 atgcacaatc aagtaactca aacgttgaag ctgtttcagc tccaacttac cataactaca
    26401 gcacttcaac tacttcaagt tcagtgagat taagcaatgg taatactgca ggtgctactg
    26461 gttcatcagc agctcaaatc atggctcaac gtactggtgt ttcagcttct acatgggctg
    26521 caatcatcgc tcgtgaatca aatggtcaag taaatgctta caacccatca ggtgcttcag
    26581 gtttattcca aactatgcca ggttggggtc caacaaacac tgttgaccaa caaatcaacg
    26641 cagctgttaa agcatacaaa gcacaaggtt taggtgcttg gggattctaa ttccttcata
    26701 ataaataaaa gtaatgttta gacattaaat atatagctct tcccatagaa attggggaga
    26761 g
//
LOCUS       DAXWFK010000015        26689 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.015, whole genome shotgun sequence.
ACCESSION   DAXWFK010000015 DAXWFK010000000
VERSION     DAXWFK010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 26689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26689
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.015"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            523..1971
                     /locus_tag="ACGEXS_000526"
     CDS             523..1971
                     /locus_tag="ACGEXS_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFF8011573.1"
                     /translation="MGSFFNKIARKEDPAIYQNKDGHLKRTLRVRDFLALGVGTIVST
                     SIFTLPGIVAAEHAGPAVALSFLLAAIVAGLVAFTYAEMAAAMPFAGSAYSWVNVLFG
                     EFFGWVAGWALLAEYFIAVAFVASGFSANLRGLVKPIGIELPAALSNPFGTNGGFIDI
                     IAAIVILLTALLLSRGMSEAARMENILVILKVLAIILFVIVGLTAINVSNYVPFIPEH
                     KVTATGDFGGWQGIYAGVSMIFLAYIGFDSIAANSAEALDPQKTMPRGILGSLSVAIV
                     LFIAVALVLVGMFHYSQYANNAEPVGWALRQSGHGVVAAIVQAISVIGMFTALIGMML
                     AGSRLLYSFGRDGLLPSWLSHLNDKHLPNRALVILTIIGVLIGSMFPFAFLAQLISAG
                     TLVAFMFVSLAMYRLRKREGKDLPIPAFKLPLYPVLPAITFVLVLLVFWGLGFEAKLY
                     TLIWFIVGIILYLSYGLRHSKKNDVAEYHPPK"
     gene            complement(2052..3389)
                     /locus_tag="ACGEXS_000527"
     CDS             complement(2052..3389)
                     /locus_tag="ACGEXS_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HFF8011574.1"
                     /translation="MSKAHQLIQEDEHYFAKSGRIKYYPLVIDHGYGATLVDIEGKTY
                     IDLLSSASSQNVGHAPREVTEAIKAQVDKFIHYTPAYMYHEPLVRLAKKLCEIAPGDF
                     EKRVTFGLTGSDANDGIIKFARAYTGRPYIISFTNAYHGSTFGSLSMSAISLNMRKHY
                     GPLLNGFYHIPFPDKYRGMYEQPQANSVEEYLAPLKEMFAKYVPADEVACIVIETIQG
                     DGGLLEPVPGYFEALEKICREHGILIAVDDIQQGFGRTGTWSSVSHFNFTPDLITFGK
                     SLAGGMPMSAIVGRKEIMNCLEAPAHLFTTGANPVSCEAALATIQMIEDQSLLQASAE
                     KGEYVRKRMDQWVSKYNSVGDVRGKGLSIGIDIVSDKKLKTRDASAALKICNYCFEHG
                     VVIIAVAGNVLRFQPPLVITYEQLDTALNTIEDALTALEAGNLDQYDISGQGW"
     gene            3647..4063
                     /locus_tag="ACGEXS_000528"
     CDS             3647..4063
                     /locus_tag="ACGEXS_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011575.1"
                     /translation="MRLKVLFHFIAAIFISFMLLWMTMLFDLISNQSHLKALLLNLDF
                     LIPSDNTPYILEIICHLLIGSVIYFVFVLLFHTSKRLYYLCYIPLFFLFIALYPFLVF
                     IAQRPIFQFSVTELIGWIITHIFFMSLMALVIPRIK"
     gene            4186..5076
                     /locus_tag="ACGEXS_000529"
     CDS             4186..5076
                     /locus_tag="ACGEXS_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002478556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HFF8011576.1"
                     /translation="MNKEQLEKMKNGKGFIAALDQSGGSTPKALKEYGVNEDQYSNED
                     EMFQLVHDMRTRVVTSPSFSPDKILGAILFEQTMDREVESKYTADYLADKGVVPFLKV
                     DKGLDEEQNGVQLMKPIDNLDNLLDRANERHIFGTKMRSNILELNEQGIKDVVEQQFE
                     VAKQIIAKGLVPIIEPEVNINAKDKAEIEKVLKAELKKGLDSLNADQLVMLKLTIPTE
                     PNLYKELAEHPNVVRVVVLSGGYSREKANELLKDNAELIASFSRALASDLRADQSKEE
                     FDKALGDAVESIYDASVNKN"
     gene            complement(5269..6765)
                     /gene="lqo"
                     /locus_tag="ACGEXS_000530"
     CDS             complement(5269..6765)
                     /gene="lqo"
                     /locus_tag="ACGEXS_000530"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase (quinone)"
                     /protein_id="HFF8011577.1"
                     /translation="MAKSNSKDIVLIGAGVLSTTFGSMLKEIEPDWNIHVYERLDRPA
                     IESSNERNNAGTGHAALCELNYTVLQPDGSIDIEKAKVINEEFEISKQFWGHLVKSGS
                     IENPREFINPLPHISYVRGKNNVKFLKDRYEAMKAFPMFDNIEYTEDIEVMKKWIPLM
                     MKGREDNPGIMAASKIDEGTDVNFGELTRKMAKSIEAHPNATVQFNHEVVDFEQLSNG
                     QWEVTVKNRLTGEKFKQVTDYVFIGAGGGAIPLLQKTGIPESKHLGGFPISGQFLACT
                     NPQVIEQHDAKVYGKEPPGTPPMTVPHLDTRYIDGQRTLLFGPFANVGPKFLKNGSNL
                     DLFKSVKTYNITTLLAAAVKNLPLIKYSFDQVIMTKEGCMNHLRTFYPEARNEDWQLY
                     TAGKRVQVIKDTPEHGKGFIQFGTEVVNSQDHTVIALLGESPGASTSVSVALEVLERN
                     FPEYKTEWAPKIKKMIPSYGESLIEDEKLMRKIRKQTSKDLELGYYEN"
     gene            7061..7390
                     /locus_tag="ACGEXS_000531"
     CDS             7061..7390
                     /locus_tag="ACGEXS_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011578.1"
                     /translation="MAYSILNLYSILIQYKCKINHKKDIQYLHPRKTLQLPLILKIIL
                     NLALIGIVIIVLTHTEFQLNAGDSNVFLLNWDIRGLTSYIFSFLILVALVSKLILIFI
                     TRRQRTS"
     gene            complement(7450..9048)
                     /locus_tag="ACGEXS_000532"
     CDS             complement(7450..9048)
                     /locus_tag="ACGEXS_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HFF8011579.1"
                     /translation="MQKSALFAPEKYNIISEIEKYSHIPDKKAILYHNTEGEDISVTY
                     QQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRT
                     KDLQYRITHGEINAVIALEPFTVEFEKIKEYDALTKFVIAGQKDGWISLEAEKENASN
                     KLEMADTTRDDMAILSYTSGTTGNPKAVTHCHGWGFAHLQMAPKHWLCINEDDLVWAT
                     AAPGWQKWVWSPFLSVLGSGATAFVFNGRFSPETYLELLQKYQINVLCCTPTEYRMMA
                     KLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTESTLLIGFLKDT
                     PQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVPLDLPALFKGYYKEPERTAKAQ
                     TGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVA
                     KPHDIRGNIVKAFVILQDHTAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNS
                     GKIRRVELRDAEIEKWQQQKDSNQ"
     gene            complement(9242..9685)
                     /locus_tag="ACGEXS_000533"
     CDS             complement(9242..9685)
                     /locus_tag="ACGEXS_000533"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HFF8011580.1"
                     /translation="MKIYDEYRSYVIEELDDCLTIQKNNDTAYYDVLEAINDLSNDSL
                     CVLNHLYINEGQEETFEQKFLRRNKHLKNVDGFKALRFLRPRTAGRHYIIITLWENRQ
                     AFYHWQNSAEYKHTHKHRGTSKGADVKIINRELSYNIRIELADMV"
     gene            complement(9966..10178)
                     /locus_tag="ACGEXS_000534"
     CDS             complement(9966..10178)
                     /locus_tag="ACGEXS_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HFF8011581.1"
                     /translation="MSFYEYIQTFKDDKTPLGELAIWIKEDDSFPKQEKLTENILSYF
                     HQMSNIDHEFLEIVKRSLSLYDQLKS"
     gene            complement(10456..12165)
                     /gene="betA"
                     /locus_tag="ACGEXS_000535"
     CDS             complement(10456..12165)
                     /gene="betA"
                     /locus_tag="ACGEXS_000535"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFF8011582.1"
                     /translation="MSNKNKSYDYVIIGGGSAGSVLGNRLSEDKDKEVLVLEAGRSDY
                     FWDLFIQMPAALMFPSGNKFYDWIYSTDEEPHMGGRKVAHARGKVLGGSSSINGMIYQ
                     RGNPMDYEGWAEPEGMETWDFAHCLPYFKKLEKTYGAAPYDKFRGHDGPIKLKRGPAT
                     NPLFQSFFDAGVEAGYHKTPDVNGFRQEGFGPFDSQVHRGRRMSASRAYLHPAMKRKN
                     LTVETRAFVTEIHYEGRRATGVTYKKNGKLHTIDANEVILSGGAFNTPQLLQLSGIGD
                     SEFLKSKGIEPRVHLPGVGENFEDHLEVYIQHKCKEPVSLQPSLDIKRMPFIGLQWIF
                     TRTGAAASNHFEGGGFVRSNNEVDYPNLMFHFLPIAVRYDGQKAAVAHGYQVHVGPMY
                     SNSRGSLKIKSKDPFEKPSIRFNYLSTEEDKKEWVEAIRVARNILSQKAMDPFNGGEI
                     SPGPEVQTDEEILDWVRRDGETALHPSCSAKMGPASDPMAVVDPLTMKVHGMENLRVV
                     DASAMPRTTNGNIHAPVLMLAEKAADIIRGRKPLEPQYIDYYKHGVHDENEGAIEVKP
                     YAK"
     gene            complement(12426..13916)
                     /gene="betB"
                     /locus_tag="ACGEXS_000536"
     CDS             complement(12426..13916)
                     /gene="betB"
                     /locus_tag="ACGEXS_000536"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFF8011583.1"
                     /translation="MELLKHLSQRQYIDGEWVESANKNTRDIINPYNQEVIFTVSEGT
                     KEDAERAILAARRAFESGEWSQETAETRGKKVRAIADKIKEHREALARLETLDTGKTL
                     EESYADMDDIHNVFMYFAGLADKDGGEMIDSPIPDTESKIVKEPVGVVTQITPWNYPL
                     LQASWKIAPALATGCSLVMKPSEITPLTTIRVFELMEEVGFPKGTINLILGAGSEVGD
                     VMSGHKEVDLVSFTGGIETGKHIMKNAANNVTNIALELGGKNPNIIFDDADFELAVDQ
                     ALNGGYFHAGQVCSAGSRILVQNSIKDKFEQALIDRVKKIKLGNGFDADTEMGPVIST
                     EHRNKIESYMDVAKAEGATIAVGGKRPDRDDLKDGLFFEPTVITNCDTSMRIVQEEVF
                     GPVVTVEGFETEQEAIQLANDSIYGLAGAVFSKDIGKAQRVANKLKLGTVWINDFHPY
                     FAQAPWGGYKQSGIGRELGKEGLEEYLVSKHILTNTNPQLVNWFSK"
     gene            13985..14080
                     /locus_tag="ACGEXS_000537"
     CDS             13985..14080
                     /locus_tag="ACGEXS_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011584.1"
                     /translation="MKNVSIYKTTQELSFVSSLDFYLTKHLTLML"
     gene            14169..14732
                     /locus_tag="ACGEXS_000538"
     CDS             14169..14732
                     /locus_tag="ACGEXS_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GbsR/MarR family transcriptional regulator"
                     /protein_id="HFF8011585.1"
                     /translation="MTRSQNKQQQLEQAKDIVINSIGQTMDLYGTNRSVGNLYGTMVF
                     EGSMTLDEMRHQLQMSKPSMSAGVKKLQEFDIVKQQFTRGSRKQHFIAEKDFFIFFRN
                     FFTKKFQREIDINVEAVKDAQAIINPLLESSDLTEAETQEAQKIKAQLDHTHVYYEWL
                     EQLTEAIESGEIFKYFPIPEQPSDSEN"
     gene            complement(14920..15606)
                     /locus_tag="ACGEXS_000539"
     CDS             complement(14920..15606)
                     /locus_tag="ACGEXS_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFF8011586.1"
                     /translation="MTNQFKNKQSKLHDSLESITKNLYATPTSELPFDNRFLFKSFIL
                     KRETGNIVIYHSGHLGDSQQDIASLGGVSKVLMNHDHESIGGSNQVEAPYFIHENDVA
                     ALKHKISVQKQFSNRVMLDKDLEVIPAPGHTPGTTLFLWDDGHHRYLFTGDFICFEGK
                     RWRTVILGSSDREKSIQSLEMVKELDFDVLVPWVTIKDEPLVYFVENEYEKREQIQNI
                     IDRVREGENS"
     gene            complement(15870..17492)
                     /locus_tag="ACGEXS_000540"
     CDS             complement(15870..17492)
                     /locus_tag="ACGEXS_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HFF8011587.1"
                     /translation="MKKEKVMDWTTFIGTVAVLLFAVIPMMAFPKASEDIITGINSAI
                     SDSIGSIYLFMGLAIFCFVMYIAFGKYGNVTLGKASDKPEFNTFTWAAMLFCAGIGSD
                     ILYWGVIEWAFYYQVPPNGAKSMSDEALQYATQYGMFHWGPIAWAIYVLPALPIGYLV
                     FVKKQPVYKISQACRPILKGQTDKFVGKVVDILFIFGLLGGAATSLALGVPLISAGIE
                     RLTGLDGKNMILRSAILLTITVIFAISSYTGLKKGIQKLSDINVWLSFVLLAFIFIIG
                     PTVFIMETTVTGFGNMLRDFFHMATWLEPFGGIKGRKETNFPQDWTIFYWSWWLVYAP
                     FIGLFIARISKGRRLKEVVLGTIIYGTLGCVLFFGIFGNYAVYLQISGQFNVTQYLNT
                     HGTEATIIEVVHHLPFPSLMIVLFLVSAFLFLATTFDSGSYILAAASQKKVVGEPLRA
                     NRLFWAFALCLLPFSLMLVGGERALEVLKTASILASVPLIVIFIFMMISFLIILGRDR
                     IKLETRAEKLKEVERRSLRIVQVSEEEQDDNL"
     gene            17624..17785
                     /locus_tag="ACGEXS_000541"
     CDS             17624..17785
                     /locus_tag="ACGEXS_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011588.1"
                     /translation="MAIIEYLYNFSRNKQNLNSAKITVLSYDLTNLTNFTRMAFKNIY
                     TLLTNIYLL"
     gene            complement(17801..17911)
                     /locus_tag="ACGEXS_000542"
     CDS             complement(17801..17911)
                     /locus_tag="ACGEXS_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011589.1"
                     /translation="MIEYDLTIILIPILKVAYPFFKKVEREKWYFSGKEV"
     gene            complement(18011..18547)
                     /gene="nrdG"
                     /locus_tag="ACGEXS_000543"
     CDS             complement(18011..18547)
                     /gene="nrdG"
                     /locus_tag="ACGEXS_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFF8011590.1"
                     /translation="MILLDIKQGQGYIAKIESNSFVDGEGVRCSVYVSGCPFNCVGCY
                     NKASQKFRYGEKYTDEILAEILDDCDHDYISGLSLLGGEPFCNLDITLNLVKAFRARF
                     GNTKTIWVWTGFLYEYLANDCTERRELLSYIDVLVDGLFIQHLFKPDLPYKGSLNQRI
                     IDVQQSLSHARMIEYIVS"
     gene            complement(18544..20394)
                     /gene="nrdD"
                     /locus_tag="ACGEXS_000544"
     CDS             complement(18544..20394)
                     /gene="nrdD"
                     /locus_tag="ACGEXS_000544"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFF8011591.1"
                     /translation="MNQIEEALTGLISKDPAIVNENANKDSDTFSTMRDLTAGIVSKS
                     YALNHLLPKHVADAHQRGDIHFHDLDYHPFQPLTNCCLIDAKNMLHNGFEIGNANVTS
                     PKSIQTASAQLVQIIANVSSSQYGGCTVDRVDELLSTYARHNEEQHRNIAKQFVKESE
                     IDRYVDQQVTKDINDAIESLEYEINTLYTSNGQTPFVTLGFGLGTDHLSRKIQQAILN
                     TRIKGLGKDRTTAIFPKLVFSIKKGTNFSPQDPNYDIKQLALKCSTKRMYPDILNYDK
                     LVEILGDFKAPMGCRSFLPSWKDAEGHFENNGRCNLGVVTLNLPRMALESAGNMTKFW
                     EIFYERIDVLHDALLYRINRLKDAVPNNAPILYKSGAFNYKLKETDDVAELFKNKRAT
                     ISMGYIGLYETATVFYGPDWETSQEAKAFTLEILKEMKRYQTKWTELYDIWFSIYSTP
                     SESLTDRFCRLDQERFGDIKDITDKGYYQNSFHYDVRKDVTPFEKLDFEKDYPYYASG
                     GFIHYCEYPKLQHNLKALEAVWDYSYDKVGYLGTNIPIDHCYECDYDGDFEATEKGFK
                     CPNCGNDNPKTVDVVKRTCGYLGNPVQRPVIKGRHKEICARVKHMKAPKE"
     gene            complement(20641..22011)
                     /locus_tag="ACGEXS_000545"
     CDS             complement(20641..22011)
                     /locus_tag="ACGEXS_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HFF8011592.1"
                     /translation="MNSDNMWLTVMGLIIIISIVGLLIAKKINPVVGMTIIPCLGAMI
                     LGYSVTDLVGFFAKGLDQVINVVIMFIFAIIFFGIMNDSGLFKPLVKRLILMTRGNVV
                     IVCAMTALIGTIAQLDGAGAVTFLLSIPALLPLYKALNMNKYLLILLLALSAAIMNMV
                     PWGGPMARVAAVLKAKSVNELWYGLIPIQIIGFILVMLFAVYLGFKEQKRIKKAIERN
                     ELPQTQDIDVHKLVEVYERDQDVRFPVKGRARTKSWIKWVNTALTLAVILSMLINIAP
                     PEFAFMIGVSLALVINFKSVDEQMERLRAHAPNALMMAAVIIAAGMFLGVLNETGMLK
                     AIATNLIKVIPAEVGPYLHIIVGLLGVPLDLLTSTDAYYFAVLPIVEQTAGQFGVPSV
                     STAYSMVIGNIIGTFVSPFSPALWLAIGLAEANMGTYIKYAFFWIWGFAIVMLVIAML
                     MGIVTI"
     gene            complement(22333..22428)
                     /locus_tag="ACGEXS_000546"
     CDS             complement(22333..22428)
                     /locus_tag="ACGEXS_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011593.1"
                     /translation="MGGFVNSLIMKDLSDNDMRFKYEYYNREKDT"
     gene            complement(22504..23109)
                     /locus_tag="ACGEXS_000547"
     CDS             complement(22504..23109)
                     /locus_tag="ACGEXS_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HFF8011594.1"
                     /translation="MNMPLMIDLTNKNVVIVGGGVVASRRAQTLNQYVEHMTVISPTI
                     TEKLQNMVDNGVVIWKEKEFEPSDIVDAYLVIAATNEPRVNEAVKKALPEHALFNNVG
                     DASNGNVVFPSALHRDKLTISVSTDGASPKLTKSIMAELEALYPPSYSSYIDFLYTCR
                     QKIKVLDITYNEKQQLLSQIVSQEYLNHDKQAQFLAWLDVR"
     gene            complement(23172..25052)
                     /locus_tag="ACGEXS_000548"
     CDS             complement(23172..25052)
                     /locus_tag="ACGEXS_000548"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HFF8011595.1"
                     /translation="MKLNTSNSPFTEKQVTEINNLLQTLTESQQQWLSGFLLANSNDT
                     TSDSNQQQLETEVWQQSQISEEQATSTTYMLQNKEPHIEANQRHVTVLYGSESGNAMR
                     LAEIFSERLSDIGHQVVLMSMDEYDTTNIAQLEDLFIITSTHGEGEPPDNAWDFFEFL
                     EDDNAPNLNHVRYSVLALGDQTYEFFCQAGKDVDVLLENLGAERICKRVDCDIDYEED
                     AEKWMADIINIIDTTSEGIQSESVISESIKSAKEKKYSKSNPYQAEVLANINLNGTDS
                     NKETRHIEFLLDDFSESYEPGDCIVALPQNDPELVEKLISMLGWDPQSPVPINDHGDT
                     VPIVEALTSHFEFTKLTLPLLKNADIYFDNEELSERIQDESWAREYVINRDFIDLITD
                     FPTIELQPENMYQILRKLPPREYSISSSFMATPDEVHITVGTVRYQAHGRERKGVCSV
                     HFAERIKPGDIVPIYLKKNPNFKFPMKQDIPVIMIGPGTGIAPFRAYLQEREELGMTG
                     KTWLFFGDQHRSSDFLYEEEIEEWLENGNLTRVDLAFSRDQEHKEYVQHRIMEESKRF
                     NEWIEQGAAIYICGDEKCMAKDVHQAIKDVLVKERHISQEEAELLLRQMKQQQRYQRD
                     VY"
     gene            25247..25339
                     /locus_tag="ACGEXS_000549"
     CDS             25247..25339
                     /locus_tag="ACGEXS_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011596.1"
                     /translation="MSVINAYSNASLLFFIYLLFIMYQISLSVK"
     gene            complement(25447..25944)
                     /locus_tag="ACGEXS_000550"
     CDS             complement(25447..25944)
                     /locus_tag="ACGEXS_000550"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFF8011597.1"
                     /translation="MNNRNVYDIEVSDYKGLTYKLEAFRGKVILVVNTATECIYSEQL
                     KKLETLFQKYKDRGFVVLSFPNNNFDNRQPGSNEEILKISREKFGVTFPVLAKISVNG
                     NDEHPLFTHLKNEQPGIFGSPIKWNFTKFIIDRQGNVVNRFLPMEDPLDISTNIEILL
                     QESSS"
     gene            complement(25954..26214)
                     /locus_tag="ACGEXS_000551"
     CDS             complement(25954..26214)
                     /locus_tag="ACGEXS_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011598.1"
                     /translation="MIIMRGYNGRYCSWTNTAIQCDYFNKVRQVKKDGKSDRLSNNIN
                     SNIIYRVLEKCITLYNVCSRCKRALILSEHSKKNIFNIKKAV"
     gene            complement(26596..>26689)
                     /locus_tag="ACGEXS_000552"
     CDS             complement(26596..>26689)
                     /locus_tag="ACGEXS_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011599.1"
                     /translation="CLNIGGQYYLQTRMLSNVDNAEKTISRNSI"
BASE COUNT         8308 a         5026 c         4000 g         9355 t
ORIGIN      
        1 aataaatatt gatatctcaa gctttagcac gtaatttgaa atttgtgaca atttttagca
       61 ttctaaaatt gcgtaacgac gctatttaaa ggctttaaca aactattgag ctttctattt
      121 ttttcatttt aaaaaggatg gctattcgac ttaaatgact tactatataa cctcctattt
      181 tccgccttac cctcatcata tttgatgcat cattttatag tcaaagaatt acatttcata
      241 caaactagcc aatcagacat aaacatgaaa ttgattttaa aaatataatg tgcaaaattg
      301 ttagaaaatt agcaaaaaat agtacttatt catttaatat ctgttataat cagttttatc
      361 atttaaaaaa tctatggatg ttatcaagac aagtcattaa tacgtaacat aaagttgtga
      421 tttttcagaa tttttagaca aaatattgat tatttgtgct atgatataac atattaaaaa
      481 acaccgtata gaattgttta tgaatgtttg ggaggaaagt ttatgggaag ttttttcaat
      541 aaaatagcac gaaaagagga tccggctatc tatcaaaata aagatggtca tttaaagcgt
      601 acacttcggg tgcgtgattt cttagcttta ggtgtaggaa caattgtatc gacatctatc
      661 tttacgctac ctggcattgt tgctgcagaa catgcaggac cggccgttgc gttatcattc
      721 ttactcgctg ctattgttgc tggtttagtt gcatttactt atgcagaaat ggctgccgct
      781 atgccatttg caggttcagc ctattcttgg gtcaatgtat tatttggtga attttttgga
      841 tgggttgccg gttgggctct attagctgaa tattttatcg ccgtagcctt tgttgcatca
      901 ggattctcag cgaatttacg cggacttgtg aaaccaattg gcatcgaatt acctgcagca
      961 ttatcaaatc catttggtac aaatggcggt tttatcgata ttattgctgc tatcgttatt
     1021 ttattaactg cattattact atcacgtggt atgtcggaag cagctcgtat ggaaaatatt
     1081 ttagttattt taaaagtatt agctattatt ttatttgtca tcgtaggttt aacagcaata
     1141 aatgttagta actatgtgcc atttattcca gaacacaaag taactgctac aggtgacttt
     1201 ggtggatggc aaggcatata tgctggtgtt tcaatgattt tcttagcgta tatcggtttc
     1261 gattctatcg cagcaaactc agcagaagca cttgatcctc aaaagacaat gcctagaggt
     1321 attcttggtt ctttaagcgt tgctatcgta ttatttattg ctgtagcact tgtgttagtt
     1381 ggtatgttcc attactcaca atacgcaaac aatgctgaac ctgttggttg ggctttacgt
     1441 caaagtggtc atggtgttgt agcagctatt gttcaagcta tctctgttat cggtatgttt
     1501 acagcattaa ttggtatgat gttagcaggc tcacgtttac tttattcatt tggacgtgac
     1561 ggcttattac cttcatggtt aagccactta aacgacaaac atttacctaa tcgcgcacta
     1621 gttatactta ctattattgg tgttttaatt ggttctatgt tcccattcgc atttttagca
     1681 caattaattt cagcaggtac acttgttgca tttatgttcg tttcattagc aatgtatcgt
     1741 ttgagaaagc gtgaaggtaa agatctacca attcctgcat ttaaattacc tttatatcct
     1801 gtgttaccag caattacatt tgttttagta ttgctagtat tctggggatt aggattcgaa
     1861 gcgaaattat acactttaat ttggtttatt gttggtatca tcctatattt aagttatgga
     1921 cttagacatt ctaagaaaaa tgacgtagct gaataccacc caccaaaata aaaaataaaa
     1981 aaaatcattt cccatctgat taacatccag atgtggaaat gattttttat tttatattaa
     2041 gataatcgct attaccaacc ttgtccagat atgtcatatt gatctaagtt acctgcttcc
     2101 aaagcagtca gtgcatcttc tatagtgttt aacgccgtgt ctaattgctc atatgttatt
     2161 accaatggcg gttggaatcg caacacattt cctgctacag ctataataac tacgccatgc
     2221 tcaaagcagt aattacaaat tttaagtgcc gcactggcat cacgtgtttt gagttttttg
     2281 tcggaaacaa tatcaatacc aatgctcaga cctttacctc taacatcgcc tacactattg
     2341 tatttagata cccattgatc cattcgtttc ctaacatatt cccctttttc cgcactagcc
     2401 tgaagaagcg actgatcttc aatcatttga attgtggcta atgcagcttc acaactaact
     2461 ggattagcac ctgttgtaaa taaatgtgct ggtgcttcta aacaattcat aatctctttg
     2521 cgtccaacaa ttgctgacat aggcatacca cctgctaagg attttccgaa agtgattaaa
     2581 tcaggcgtaa aattaaaatg cgagactgaa ctccatgtac ctgttctccc aaaaccttgt
     2641 tgaatatcat cgacagcgat taaaatacca tgttcacgac aaatcttttc taacgcttca
     2701 aaataccctg gaactggttc taaaagtcca ccatcgcctt gtatcgtttc aataacaata
     2761 catgctactt cgtcagcagg tacatacttc gcaaacattt cttttaaggg tgctaaatat
     2821 tcttctactg aattagcttg tggctgctcg tacataccac gatatttatc tggaaacgga
     2881 atatgataaa aaccattcaa taacggacca taatgtttgc gcatatttaa actaatagct
     2941 gacatagaca atgagccaaa agttgaacca tgatatgcat tagtgaaact aatgatataa
     3001 ggacgccctg tatatgctct ggcaaattta atgatgccat catttgcgtc tgatccggtt
     3061 aatccgaagg tcactctttt ttcaaaatct ccaggtgcaa tctcacaaag cttcttagct
     3121 aaacgtacca acggttcatg atacatatat gctggtgtat aatgaataaa tttatcaact
     3181 tgtgccttta tcgcttctgt tacttctcta ggtgcatgac ctacgttttg agaactcgca
     3241 ctcgataaca aatcgatata tgtcttcccc tcaatatcaa ccaatgttgc tccatatcca
     3301 tgatcaatca ctaacggata atatttaata cgtcctgatt tcgcaaaata atgttcatcc
     3361 tcttgaatta attgatgtgc tttactcata tacattcccc tcccattgac agtattaatg
     3421 tgcaagcaaa gtggcctctc ttaaatcgat tataaaatcc gatttaaaat cctgctcaaa
     3481 taccgtcatg cctctatgaa tactatccaa tatactaata aaattcagat ttgtaaatat
     3541 attctgataa ttaagaattt tatatcaatc tactttgttt atgcgcataa ttttgttcga
     3601 ttagggaatt gtttactata ttccatttga ctctgaggtg atacgaatgc gtttaaaagt
     3661 attgttccat ttcattgctg ctattttcat atcattcatg ttgctatgga tgaccatgtt
     3721 gtttgactta ataagcaatc aatcacattt aaaagcttta ttattgaatt tagattttct
     3781 aattccttct gacaatacac catatattct agaaataata tgtcacttac taattggtag
     3841 cgtcatttat tttgtatttg tgttgctgtt tcatacttct aaaaggcttt actatctatg
     3901 ttatatccct ttatttttct tatttatagc attgtatccc tttttagtat ttattgcgca
     3961 acgtccaatt tttcaattta gtgtgacaga attaatcggt tggattatta cacatatttt
     4021 ctttatgtca ttaatggcgt tagtcatacc tagaataaaa taatattttt aattaaactg
     4081 acaattattt ttcacatttt atacactttt caaggtttat gacttgagaa atgttctcat
     4141 ttttatcata ctggaggtgt attgaaaagt taaggagtgg aaagtatgaa taaagagcaa
     4201 ttagaaaaaa tgaaaaatgg aaaaggcttt attgccgcat tagaccaaag tggtggtagt
     4261 acaccaaaag cacttaaaga atatggtgta aacgaagatc aatatagcaa tgaagacgaa
     4321 atgttccaac ttgttcacga tatgcgtaca cgtgtggtaa cttcaccttc attctcacca
     4381 gataaaattt tgggtgctat tcttttcgaa caaacaatgg atcgcgaagt agaaagcaaa
     4441 tacactgcag attacttagc tgataaaggt gttgttccgt tcttaaaagt agacaaaggt
     4501 cttgatgaag agcaaaatgg tgttcaatta atgaaaccaa tcgacaactt agacaattta
     4561 ttagaccgtg caaacgaacg tcacattttt ggtacaaaaa tgcgttctaa cattttagaa
     4621 ttaaatgagc aaggtatcaa agacgttgtt gaacaacaat ttgaagttgc taaacaaatt
     4681 attgctaaag gtttagttcc aattatcgaa ccagaagtta atattaatgc aaaagacaaa
     4741 gctgaaattg aaaaagtatt aaaagctgaa cttaaaaaag gtttagatag cttaaatgct
     4801 gatcaattag taatgttgaa attaacaatt cctactgaac caaacttata caaagagtta
     4861 gctgaacatc ctaatgttgt tcgtgtcgtt gtattatcag gtggttacag cagagaaaaa
     4921 gcaaatgaat tacttaaaga taacgctgaa cttatcgcaa gtttctcacg tgcattagct
     4981 agtgatttaa gagctgatca atctaaagaa gaattcgata aagctttagg tgatgctgta
     5041 gaatcaatct acgacgcatc tgtaaacaaa aactaattta tacattaaac tattttttga
     5101 tataaactac taaaaaggac tcatacatgt gctttaaagg cattgtgtat gagtcttttt
     5161 tgattaaagt tgattttgcc gtatgtatta gattacgtaa aaaaatcgga cagctgaaaa
     5221 atctttaaga tttctcagtt gtccgatttt aaatatagct attagaattt agttttcgta
     5281 gtaacctaat tctaagtctt ttgaagtttg tttacggatt tttctcatta atttttcgtc
     5341 ttcaattaat gattcaccgt atgatggaat cattttctta attttaggtg cccattcagt
     5401 tttgtattca gggaagttac gttctaatac ttctaacgca actgaaactg aagttgaagc
     5461 ccctggtgat tcacctaata atgcaattac agtgtggtct tgtgagttaa ccacttctgt
     5521 accgaattgg atgaatcctt taccgtgttc aggtgtatct ttgataactt gtacacgttt
     5581 accagcagtg tataattgcc aatcttcatt acgtgcttct ggatagaaag tacgtaagtg
     5641 gttcatacaa ccttcttttg tcataattac ttggtcaaat gagtatttaa ttaaaggtaa
     5701 gtttttaact gctgctgcta ataaagttgt aatgttgtat gttttaacag acttgaataa
     5761 atctaagtta gaaccatttt tcaagaattt aggtccaaca ttagcaaatg gtccaaataa
     5821 taatgttctt tgaccatcaa tgtaacgcgt atctaaatga ggtacagtca ttggtggtgt
     5881 accaggtggc tctttaccat aaactttggc atcgtgttgt tcaataactt gtgggtttgt
     5941 acaagctaag aattgaccac tgatagggaa tccacccaaa tgtttacttt cagggatacc
     6001 tgttttttgt aataatggaa ttgctccacc gccagcaccg atgaatacgt agtcagttac
     6061 ttgtttgaat ttctcaccag ttaggcgatt tttaacagta acttcccatt gaccatttga
     6121 taattgttca aaatcaacaa cttcatggtt aaattgcact gtagcatttg gatgtgcttc
     6181 aatgctttta gccattttac gtgttaattc accgaagttt acatctgtac cttcgtcaat
     6241 tttacttgcc gccatgatac cagggttatc ttcacggcct ttcatcatca atggaatcca
     6301 ttttttcatt acttcgatgt cttcagtata ttcgatatta tcgaacatag ggaaagcttt
     6361 catcgcttcg taacgatctt ttaagaattt aacattgttt ttacctctaa cataactgat
     6421 gtgtggtaat ggattgataa attctcttgg gttctcgatg ctaccgcttt tcactaagtg
     6481 accccagaat tgttttgaaa tctcaaactc ttcgttaatc actttcgctt tttcgatgtc
     6541 gatagaacca tcaggttgta aaactgtgta gttcaactca cataatgctg catgacccgt
     6601 accagcatta tttctttcgt ttgaactttc gattgcagga cgatccaagc gttcgtaaac
     6661 gtggatattc cagtctggct caatttcttt taacattgaa ccaaatgttg tgctaagtac
     6721 accggctcca attaaaacga tgtctttact attagactta gccattggtt tcacctctcc
     6781 aaaaattgta aatgtgtatc atcaatatga aagttacata aaactgacat atttctttaa
     6841 aatatcaacg ccattgataa cttcctgttt taattgatac gctgtaacaa aatactatag
     6901 ttagtgctta catgtatatg ttaaagcaag cagtggtaaa tgtaaattat aattattcat
     6961 taactttgca atatattaaa tcttttattc atagaagata aatatcaaat caatcataat
     7021 tatttgacaa caaatagcta acgattgttt taatctacat ttggcttata gcattttaaa
     7081 cctatactct attttgatac aatataagtg taaaatcaat cataaaaagg atattcaata
     7141 tctgcatcca agaaaaacat tacaattacc tttaatattg aaaattattt tgaatcttgc
     7201 acttataggt attgtaatta tcgttctaac acatactgag tttcaactta atgccggtga
     7261 tagtaatgtg tttttattaa actgggatat tcgaggttta acatcttata ttttctcatt
     7321 cttaatactg gttgcgcttg tttctaaact aattttaata tttataacta gacgacaaag
     7381 aacatcataa ataaacaagc agtccagtgc gtatcatcat accttacgca ttggactgct
     7441 ttgctatgtt tattgattgc tatctttttg ttgttgccat ttttctattt cagcatctcg
     7501 tagttcaaca cgacgaattt tacctgagtt tgtttttggt aagtcgtcaa cgaattcaat
     7561 ctctctcgga tatttatatg gtgcaacttc atttttaaca aattgttgta gttctttaac
     7621 taacgtatca tcacccgcag tatggtcctg taaaataacg aatgctttaa caatatttcc
     7681 tcgtatatca tgaggttttg ctactacagc acattcttta acggctggat gattcgttaa
     7741 cgcatcttca acttcaaaag gcccaatcgt atagcctgaa ctaataataa tgtcatctcg
     7801 acgtccttca aaccagaaat aaccatcatc atctacatga gctaagtcac cagtgatgta
     7861 gtatttacct gtttgcgctt tcgccgtacg ttctggctct ttataatacc ctttgaaaag
     7921 tgctggcaaa tcaagtggta ctgcaatatt ccctttcgta ttagcaggta cgctattccc
     7981 ctcatcatct actacagtga ccgaactacc cggaatgcct ttacccattg atccaatcct
     8041 ctgtggtgta tcttttaaaa agcctataag caaggtactt tcagtctggc catatccatc
     8101 tcttacagtt aaattaaagt atttcttgaa ttgttcaact acttctcgat ttagtggctc
     8161 acctgcagaa acggcactat gtaaatgcgt taagtcataa tcatttaagt tctgtaattt
     8221 agccatcata cgatattctg tcggtgtaca acataaaaca ttaatttgat atttttgaag
     8281 caattctaag tatgtttcag gactgaacct tccattaaat acaaaagcag ttgcacctga
     8341 acctaataca gataagaaag gactccatac ccatttttgc caacctggtg ctgctgttgc
     8401 ccaaactaag tcatcttcat taatacataa ccaatgtttt ggtgccattt gtaaatgtgc
     8461 aaatccccat ccatgacaat gtgtaacggc tttaggattg ccagttgtac cagatgtata
     8521 tgacagaatc gccatatcat cacgcgtcgt atctgccatt tctagtttgt tacttgcgtt
     8581 ttctttttca gcttcaagtg aaatccatcc atctttttga ccggcaataa caaatttagt
     8641 taacgcatca tattctttaa ttttttcaaa ttcaactgtg aatggctcta gtgcaataac
     8701 tgcattaatt tcaccatgtg tgatacggta ttgtaaatct ttagttctta gcatttcaga
     8761 acatggaatg attgcaacac ctaattttaa agcagcaata tataattcat acgtcgcaat
     8821 agatcgtggc atcataatga gtactttatc gcctttagat aaaccgtgcg atgctaaaac
     8881 attacctact ttattagact gttcaatgag ctgttggtaa gtgactgata tatcttcgcc
     8941 ttcagtatta tgatataaaa ttgccttttt atctggtatg tggctatatt tttcgatttc
     9001 cgaaataatg ttatattttt caggcgcgaa tagagctgac ttttgcataa ctaacttcct
     9061 ttcatacatc cacttttcct gtgatgaaca ttgtaatttt ataaatgaat tatatacatc
     9121 atacgcctat ctttacagaa ttttcaatta aatagggtta aataccaaag tcctcgacac
     9181 tacactttga catgacgtag cattcaagga ctttcaaatg attgagggtt gatatctcgg
     9241 gctagaccat atcagctaat tcaatacgaa tattgtatga taattcacga ttaattattt
     9301 ttacatctgc acctttcgaa gtgccacgat gcttgtgtgt atgcttgtac tcagctgaat
     9361 tttgccaatg ataaaatgct tgcctatttt cccacagcgt aataatgata tagtgtctac
     9421 cagctgttct aggtcttaaa aaccttaatg ctttaaatcc atcaacgttt tttaaatgct
     9481 tattacgtcg caaaaatttt tgctcgaatg tttcttcttg tccttcgtta atatataaat
     9541 gatttaacac acataatgaa tcatttgata aatcatttat cgcttctagc acatcgtaat
     9601 atgcagtgtc attatttttt tgtattgtga gacaatcatc cagttcttct attacataac
     9661 ttctatattc atcataaatt ttcataataa atgccttcat ttcatttata tttttggtca
     9721 tattacttta tatctattac taattcattc ccgtatttat taattacaat catagtttgg
     9781 cttcttttta aaagataaga ctttgtaaaa agtattaata tttcatgcaa atgggggaca
     9841 ggagtcgccc actatttttg tgtcttcaat ttcatgatca ttatttaaca ttagtcatga
     9901 aaatagccga caacttcatc tgtgaaatca ccggcctttt attttagcta actttatttc
     9961 tgattttacg attttaattg atcatacaga gaaagtgatc tttttacaat ttctaaaaac
    10021 tcatgatcta tattggacat ttgatgaaaa taagacaaaa tgttttctgt tagcttctct
    10081 tgttttggga atgaatcatc ttctttaatc caaatcgcta attcgcctaa tggtgtttta
    10141 tcatctttaa atgtttgtat atattcgtaa aagctcatag tattccttct ctcaatttac
    10201 ttatataaat cctaccacga aagctttcaa gaaaacacaa ttaaatgtct atttagtgaa
    10261 ctttttaagg ttgtgcactc ttttaatgtc tgccaattag gtcaattaat catcacaatg
    10321 tacaattaac tctattttca gttcatatac tcacacaccg tttttgaaca acacattaac
    10381 ttctcattta gataaaacgc aaaaaagcct ggcaccaata caatagatgc cagactaaga
    10441 gtctactata taaatttatt tagcgtatgg ttttacttcg attgcacctt cattttcatc
    10501 atgaacacca tgcttataat aatcaatata ttgtggctct aaaggctttc tgccacgtat
    10561 aatgtctgct gctttttcag ctaacattaa aacaggtgcg tgtatattgc catttgtcgt
    10621 acgtggcata gctgatgcat caactacacg taaattttcc ataccgtgga ctttcattgt
    10681 taacgggtca actactgcca ttggatctga agcaggaccc attttagcac tacaagatgg
    10741 gtgtaatgct gtttcaccat ctctacgaac ccaatcaaga atttcttcgt ctgtttgcac
    10801 ttctggtcct ggtgaaattt ctccaccatt gaatggatcc attgcttttt gagataagat
    10861 atttcttgct acacgaattg cttctaccca ttctttttta tcttcttctg ttgataaata
    10921 attaaagcgg atacttggtt tttcgaatgg atctttagat ttgattttca agctaccacg
    10981 agagtttgaa tacattggtc ctacgtgaac ttgataacca tgtgcgaccg ctgccttttg
    11041 accatcatat cttacagcta ttggtaagaa atggaacatt aagttaggat aatcaacttc
    11101 gttatttgaa cgtacaaatc cgccaccttc aaaatggtta gatgctgctg cacctgtacg
    11161 tgtgaaaatc cattgtaaac caataaatgg catgcgcttg atatctaagc ttggctgtaa
    11221 tgatacaggt tccttacatt tatgttgaat gtatacctct aagtgatctt caaagttttc
    11281 acccacacca ggtaaatgaa cacgtggctc aatgcctttt gattttagga actctgaatc
    11341 accgatacca gataattgta gtaattgtgg cgtattgaat gccccaccag acaaaatgac
    11401 ttcattagca tcgatggtat gtagtttgcc atttttctta tacgtaacac cagttgctct
    11461 tctaccttca taatgaattt cagttacaaa ggcacgtgtt tcaacggtta agtttttacg
    11521 cttcatcgct ggatgtaaat atgctcttga agctgacatt cggcgaccac gatgtacttg
    11581 actatcgaac ggtccaaaac cttcttgtct aaatccattc acatcaggtg ttttatgata
    11641 gcctgcttca acacctgcat caaagaatga ctggaataaa ggattcgttg ctggccctcg
    11701 ttttaactta attggtccat catggcctct aaatttatca taaggcgctg caccgtatgt
    11761 tttttctaat tttttaaaat acggtaaaca gtgcgcaaaa tcccaagttt ccataccttc
    11821 tggttctgcc cagccttcat agtccattgg attaccacgt tgataaatca tgccattaat
    11881 cgaacttgat ccacctaaaa ctttacctcg ggcatgtgct actttacgac cgcccatatg
    11941 tggttcttca tctgttgaat aaatccaatc gtaaaattta ttgcctgaag ggaacattaa
    12001 cgcagcaggc atttggataa ataaatccca aaaataatca ctgcgacccg cttctaatac
    12061 taagacttct ttatctttat cttcactcag acgattacct agtacagaac ctgcactgcc
    12121 tcctccaatg atgacataat catatgattt gtttttgtta ctcatcgtta tgacctccaa
    12181 agtttgttgt atttcaaagt gagtattgca tgaaatattt gttagtggct gattggttat
    12241 cgttgaattg ctaaacttag ctaaccaaaa cactcgcttt tccttttacg attgttacta
    12301 acagtaatga aataatctat gattcatttt gcgtttagtc caaattatca atcaacattt
    12361 cgccacttat acaaaacaaa gtgagtgtct tgtgttctta caatggcact caccttatct
    12421 aatttttatt tgctaaacca attcactaat tgtggatttg tatttgttaa aatgtgtttt
    12481 gaaacaaggt actcttctaa gccttctttg cctaattctc taccgatacc tgattgtttg
    12541 tatccacccc atggcgcttg tgcaaaatat ggatggaaat cattaatcca caccgttcca
    12601 agtttcaact tgttagcaac gcgttgtgct tttccaatat ctttagaaaa tacagcacct
    12661 gctaaaccat atatagaatc attcgctaat tgaatcgctt cttgttcagt ttcaaagcct
    12721 tctacagtaa cgacaggtcc gaaaacctct tcttgtacaa tacgcattga cgtatcacaa
    12781 tttgtaatga ctgttggctc gaagaataga ccatctttta aatcatctct atctggacgt
    12841 ttaccaccaa cagcaattgt tgcgccttct gctttagcta catccatata agattcgatc
    12901 ttattacgat gttctgttga aatcactggt cccatttcag tatcagcatc aaaaccatta
    12961 cctaatttga tttttttcac gcgatcaata agtgcttgct caaatttgtc tttaatactg
    13021 ttttgtacta atattcttga tcctgctgaa caaacttgac ctgcatggaa atatccacca
    13081 tttaacgctt ggtctactgc caattcaaaa tcagcatcat caaagataat gtttggattt
    13141 ttaccgccaa gttccaaggc aatattcgta acattattag cagcattttt cataatatgc
    13201 ttaccagtct caatgccacc tgtaaatgat acaaggtcaa cctctttatg acctgacatt
    13261 acgtcaccaa cttcagaacc tgcacctaga ataagattaa ttgttccttt agggaaacca
    13321 acttcttcca ttaattcaaa aacacgtatt gttgttaatg gtgtaatttc acttggtttc
    13381 ataactagtg aacaacccgt agcaagcgct ggcgcaattt tccatgatgc ttgtaataac
    13441 ggataattcc aaggtgtaat ttgtgtaact acacctactg gttctttaac aattttgctt
    13501 tctgtatctg gaattggtga atcaatcatt tcgccaccgt ctttatctgc taatccagca
    13561 aaatacataa acacattatg aatatcatcc atatctgcat atgattcttc taacgttttt
    13621 ccagtatcta atgtttctaa tcgtgctaac gcttcgcgat gttctttaat cttgtccgcg
    13681 atggcacgta cttttttacc tcttgtttca gcagtttctt gcgaccattc accagactca
    13741 aacgcacgtc ttgcagctaa gattgcacgt tctgcatcct cttttgtccc ttcagaaacc
    13801 gtaaatatca cttcttgatt gtaaggattg ataatatctc ttgtattttt attcgcgctt
    13861 tcaacccact caccatcaat atattgacgc tgagataaat gttttaaaag ttccattcaa
    13921 caacctccgt taagtttgtt aaatttttat ttaacgaatt ttacaaactt atgttagcat
    13981 agaaatgaaa aatgtgtcaa tttacaaaac aactcaagaa ttaagttttg taagttcgtt
    14041 agatttttat ttaacgaaac atttaacttt aatgctttaa atatttttaa tgtctttttt
    14101 tagaaaaggt tttataatgt attagtattt attttaacgt gaccaaaatg atgtacggag
    14161 gtgcaatgat gactcgttct cagaacaaac agcaacaatt agaacaagca aaagatattg
    14221 taattaattc aattggacag acaatggatt tgtatggcac taatagaagt gtaggaaact
    14281 tatatggcac aatggtgttt gaaggcagta tgacgcttga tgaaatgcgt catcaattgc
    14341 aaatgagcaa accaagtatg agtgctggcg ttaagaaatt gcaggaattt gatattgtta
    14401 aacaacaatt tacgagagga agccgcaaac aacattttat cgccgagaaa gactttttca
    14461 tctttttccg aaactttttc actaaaaaat tccagcgtga aattgacatc aatgtcgaag
    14521 cagtaaaaga tgctcaagct attatcaatc cattacttga aagcagtgat ttaactgaag
    14581 ctgaaacaca agaggcacaa aaaattaaag ctcagcttga tcatacacat gtttattacg
    14641 aatggttaga acagttaacc gaagcgattg aaagcggtga aattttcaaa tatttcccaa
    14701 ttccagaaca gccttctgat tctgaaaatt aaaattgact caaacaacga caatgatgat
    14761 tatgcatgat aaaaacaatg caataggtcc acgaaataaa cggtaatttc tatctcaata
    14821 aatagaaatt accgtttttg ttttatatga caatcaatta tggaggtatt ttctagggct
    14881 ttcttagatt cgttacattg cttcgccaat atatttcaat tagctattct cgccctcacg
    14941 tactctatca ataatatttt gtatttgttc acgtttttca tattcatttt ctacaaaata
    15001 aactaacggt tcatctttga tagtaaccca aggtacaagt acatcaaaat ctaattcttt
    15061 aaccatctct aaactttgaa tagatttttc tctatcactt gaacctaata taactgtacg
    15121 ccatctcttc ccttcaaaac atataaaatc tccagtaaat aagtaacggt gatgaccatc
    15181 atcccataaa aatagtgtcg tccctggtgt atgtccaggc gcgggaataa cttctaaatc
    15241 cttatccaac attacacgat tactaaattg tttttgaaca gaaatcttat gtttcagtgc
    15301 agccacatca ttttcatgta taaagtaagg ggcttcaact tgattagaac ctcctataga
    15361 ttcatgatca tgattcatca atacttttga aacaccacct aatgatgcaa tgtcttgttg
    15421 ggagtcacct aagtgtccgg aatgatagat aacaatatta cctgtttcac gttttaaaat
    15481 aaaagattta aatagaaatc gattatcaaa aggcagttcc gaagtaggtg tcgcatataa
    15541 gttttttgtg atggattcta aactgtcatg taatttggac tgtttatttt taaattgatt
    15601 agtcatttta tttcatcctt cgattaattt gaattgtaca tacgcagtac cctttattta
    15661 atttaataat caatgagagt ctttcaaact tcgatttaga atatgcttca tacaataaat
    15721 aagtacttgg agcactaaaa aattaaatct tttagtacaa cattaatgaa aacctaaaag
    15781 ttcatcctac aatgctacta aaaaaagggg aatggaacag aaatgatatt ttcacaaaat
    15841 tcatttcgtc gtcccagacc cgctttgaat tataaattat cgtcttgttc ttcttctgat
    15901 acttgaacga ttcgcaatga acgacgttca acttctttta atttttcagc acgcgtttca
    15961 agtttaattc tatcgcgccc taaaatgatt aaaaatgata tcatcatgaa aataaaaata
    16021 acaattaatg gcacacttgc cagtattgaa gcagttttca atacttctaa tgcacgttca
    16081 ccaccaacta gcatcaatga aaatggcaat aagcacaatg caaatgccca gaataaacga
    16141 ttggcacgta atggttcgcc taccactttt ttctgagatg ctgccgctaa aatatatgaa
    16201 cccgaatcaa atgttgttgc taagaataag aaagcagata ctaagaatag tacaatcatc
    16261 aatgatggga atggtaaatg atgcaccact tcaataatgg ttgcctctgt accatgtgta
    16321 tttaaatatt gtgttacatt aaactgtcca gaaatttgta aatacacagc atagttacca
    16381 aaaataccaa agaataatac gcatccaagc gttccataaa taattgttcc tagcacgact
    16441 tctttaaggc gtcgaccttt tgaaattcta gcgataaata aaccgataaa tggcgcatat
    16501 actaaccacc atgaccagta gaatattgtc cagtcttgtg ggaaattcgt ttcttttcga
    16561 cctttaatac caccgaatgg ttctaaccat gttgccatat gaaagaaatc tctcaacata
    16621 tttccgaacc ctgtcactgt cgtttccata ataaaaacag tcggtccaat aataaatata
    16681 aaggctaaaa gtacaaagga tagccaaacg ttgatatcac ttaacttttg aatacctttt
    16741 ttcaatcctg tatatgaact aatggcaaat ataaccgtga ttgttaataa aatggccgaa
    16801 cgtaaaatca tatttttacc atctaaacca gttaatcttt ctatgcctgc agaaattaat
    16861 ggcacaccta acgctagtga tgttgccgca ccacctagca atccaaagat aaataagata
    16921 tctacaactt tacctacaaa tttatctgtt tgacctttta aaatcggacg acaagcttga
    16981 ctaattttat acaccggttg ttttttaaca aatactaaat aaccaattgg taatgctggt
    17041 agaacataaa tagcccaagc aattggcccc cagtggaaca taccatattg cgtcgcatat
    17101 tggagtgctt catcactcat acttttcgcg ccatttggtg gaacttgata gtaaaaagcc
    17161 cattcaataa cgccccagta taaaatatca gagcctatgc ctgcacaaaa cagcattgcc
    17221 gcccatgtaa atgtattaaa ttctggttta tcacttgctt taccaagtgt gacattacca
    17281 tatttaccaa atgcgatata cattacaaag caaaaaatcg ccagccccat aaataaatat
    17341 atcgaaccaa ttgaatcaga aatggcacta ttaataccag tgatgatatc ttcacttgct
    17401 tttggaaaag ccatcatagg tataactgca aaaagaagta cagctactgt ccctataaag
    17461 gtcgtccagt ccataacttt ctcttttttc aattgtgctc cccctaatta ttaattttat
    17521 gaatcctgtt tcgatttatc tcaaaatgta ataattatat tgattcacaa aattgacaat
    17581 aactaacatt taataataat gcaaatttca tacaatttga aacttggcaa ttattgaata
    17641 tttatataat ttttcccgta ataaacaaaa ccttaatagc gctaaaataa cagtgttaag
    17701 ttacgattta acgaatttaa caaattttac tagaatggca tttaagaata tttatacgtt
    17761 attaacgaat atttatttat tgtaaaacgc taccaaaaag ttagacttcc ttcccactaa
    17821 aataccactt ttctctttca acttttttaa aaaacggata tgcaactttt agtattggta
    17881 tcaaaatgat tgttaggtca tattctatca atatattttt ataaagaatt gcttttatta
    17941 actttcaatt atgtacctaa cctaaaaaga agccaaggca acgaatgtta ccttgacttc
    18001 taatacatat tcaactaact atatattcaa tcatacgcgc atgcgagagt gattgttgta
    18061 catctataat gcgttgattt aaagaacctt tatatggtaa atcaggtttg aataagtgtt
    18121 gtataaatag accatctact aaaacgtcaa tgtatgataa taactctcga cgttctgtac
    18181 aatcatttgc taaatattca tataaaaatc cagtccatac ccaaattgtc tttgtatttc
    18241 caaaacgtgc tcgaaatgct ttgacaagat ttaatgtaat atccaaatta caaaatggtt
    18301 cgccacctaa tagacttagc ccagatatat aatcatgatc gcaatcatct aatatttctg
    18361 ctaatatttc atcagtgtat ttctcgccat atctgaactt ttgtgaggct ttgttataac
    18421 atccaacaca attaaatgga catcctgata cataaacact gcatcttact ccttcaccgt
    18481 caacaaagct atttgattct attttagcaa tataaccttg tccttgttta atgtctaaaa
    18541 gtatcattct ttaggcgctt tcatatgttt tactcgtgcg caaatttctt tatgacggcc
    18601 tttaattact ggacgttgaa ctggattgcc taggtaacca catgttcgtt taacgacatc
    18661 aactgtttta ggattatcat tgccacagtt cgggcattta aatccttttt cagttgcttc
    18721 aaaatctcca tcgtaatcac attcataaca atgatcaatc ggaatatttg tacctaagta
    18781 accaactttg tcataagagt agtcccatac cgcttctagt gctttcaaat tgtgttgcaa
    18841 tttcggatac tcacaatagt gaatgaaacc accactcgca taataaggat aatctttttc
    18901 aaaatctaac ttttcaaaag gtgtaacatc tttacgtaca tcataatgga aagagttttg
    18961 ataatatcct ttatctgtaa tgtctttaat atctccaaat ctctcttggt ctaaacgaca
    19021 aaaacgatcc gttagcgatt cactcggcgt actgtaaata ctgaaccaaa tgtcatataa
    19081 ttctgtccat ttcgtttgat aacgtttcat ttctttaaga atttcaagcg taaatgcttt
    19141 tgcttcttga gatgtttccc agtctggacc atagaaaaca gtagctgttt catacaaccc
    19201 tatatagccc attgaaatcg ttgcacgttt atttttaaat aactcagcaa catcatctgt
    19261 ttcttttaat ttatagttaa atgcgccact tttatataaa atcggtgcgt tattcggtac
    19321 agcatctttc aaacgattta tacgataaag taatgcatca tgtaacacat cgatacgttc
    19381 ataaaagatt tcccagaatt tcgtcatatt accggcagat tctaatgcca ttctaggtaa
    19441 attaagtgta acaacaccaa gattacaacg accattattt tcaaaatgac cttccgcatc
    19501 tttccaactt ggtaaaaatg aacgacaacc cattggcgct ttgaaatcac ctaatatttc
    19561 tacgagtttg tcataattta aaatatctgg atacatacgt ttcgttgaac actttaatgc
    19621 tagttgttta atgtcatagt tcggatcttg cggactaaag ttggttcctt ttttaattga
    19681 aaatacaagt ttcgggaaaa tcgctgtcgt gcggtctttt cctaagcctt tgatacgagt
    19741 atttaagata gcttgttgaa ttttgcgact taaatgatct gtacctaagc cgaatcctaa
    19801 tgttacaaaa ggtgtctgtc cattagatgt atataaggta ttaatttcat attctaaact
    19861 ttcaatcgca tcattgatgt ctttagtgac ttgttgatca acataacgat caatttcaga
    19921 ttctttgaca aattgctttg cgatattcct atgttgttct tcattatgtc gtgcatatgt
    19981 actaagtaat tcgtcaacgc ggtcaaccgt acagccacca tattgactgc tagaaacatt
    20041 ggctataatt tgtacaagct gcgctgatgc agtttgtatt gattttggtg aagttacatt
    20101 cgcgttgcct atttcaaatc cattatgtag catattttta gcatctatta aacaacagtt
    20161 agttaacggt tggaatggat gataatctaa gtcgtgaaaa tgtatgtccc ctctttgatg
    20221 tgcatctgca acgtgctttg gtaataaatg atttaatgcg taagatttag aaacgatacc
    20281 tgctgttaaa tctctcattg ttgaaaatgt atcactatct ttgttagcat tttcgttaac
    20341 aatagcagga tctttagaaa tcaaacccgt taatgcttct tcgatttgat tcattttacc
    20401 ccttcttcct atatattgtg tctactcatt acgttatacc actatatata gtattttcaa
    20461 atttttatta gtctgttaac taaaacgtca caaagttatt aacatttagt gaaattcata
    20521 tttctacaca attatttcat taatttttaa aattgtttaa agattcgtcc gtcatttcga
    20581 catgtttatg caactattat ttcattttgc aacgtgacca tagtttctat tttttcatac
    20641 ttaaatcgtc acaatgccca tcaacattgc aattactaac ataacgatag cgaatcccca
    20701 aatccaaaag aatgcatact taatatacgt gcccatgttt gcctctgcta aaccaattgc
    20761 caaccataag gctggtgaaa atgggctgac aaatgtacct ataatattcc ctatgaccat
    20821 tgaataagct gttgatacag acggtacacc aaattgccct gctgtttgtt cgacaatcgg
    20881 taacacagca aaataataag cgtctgtgct agttagtaaa tctaatggta cgccaagtaa
    20941 acctacaata atatgcaagt atggtcctac ttctgcagga atcactttga ttaaattggt
    21001 cgcaatcgct ttaagcatac cggtttcatt tagtacacct aaaaacatac ctgctgcaat
    21061 aatcactgca gccatcatta atgcattcgg cgcatgcgct cttaatcgtt ccatttgttc
    21121 atccactgat ttaaaattaa taacaagtgc caacgaaaca cctatcatga atgcaaattc
    21181 aggtggcgca atatttatta acatcgatag aataacagct aaagttaaag ctgtattcac
    21241 ccattttatc catgattttg ttcttgcacg tccttttaca ggaaacctta catcttgatc
    21301 tcgttcatat acttcaacta atttatgtac atctatatct tgtgtttgcg gtaattcatt
    21361 tctctctatt gcttttttga tacgtttctg ttctttaaat ccaagatata ccgcaaacaa
    21421 cataacaaga atgaaaccta ttatttgaat aggtattaat ccataccata attcattgac
    21481 acttttggct tttaacactg cagctacacg agccattgga cctccccaag gtaccatgtt
    21541 cataatcgcc gcgcttaatg ctaatagtaa aatcaataaa tatttattca tatttaacgc
    21601 tttatataaa ggtaataatg caggaataga aagcaaaaat gttaccgcac cggccccatc
    21661 taattgggct attgtgccaa ttaaagctgt cattgcacag acaatgacga cattgcctcg
    21721 tgtcattaat attaagcgtt tgacaagcgg cttgaataaa ccactatcgt tcatgatgcc
    21781 aaagaaaata atggcaaaga taaacataat aacaacgttg atgacttgat ctaacccttt
    21841 agcaaaaaat ccaaccaaat ctgtcacact atatcctaaa atcattgccc ctaagcaagg
    21901 tatgattgtc atacctacaa ctggatttat ctttttggca atgagtaaac ctacaattga
    21961 aataataata atgagcccca ttactgttaa ccacatatta tcactattca tattattttc
    22021 tccctccact tttaatacaa tttacataat ccccctcttt aacttaaata ttataattaa
    22081 tataattttt tatatttaag ttatctcatt ttatcaaaaa aatctttttt gcgtaactat
    22141 caatagtgaa gtttgatttt cctacttgat aacatggcat gactaatcct tgtatatcct
    22201 gtcctgtatt tcattttgat ctatcatttt tattattgct ttctattgca caaaaaatct
    22261 ggctaaaggt cgctatgata gtaacgactt caaccagatt tcaatttatc cacctatttt
    22321 attaagtttg ttctacgtat ctttttctct attgtaatat tcatatttaa aacgcatatc
    22381 attatcgctt aaatccttca ttattagact attcacaaag ccgcccatgt cttaatttca
    22441 atgattaagc gttaaagtta aaataagaag acttacctta gacggttaga cggtccgcta
    22501 ttattatctt acatccaacc acgctaaaaa ttgagcttgt ttgtcatgat ttaaatattc
    22561 ttgtgacaca atttgtgaca gtaactgttg cttttcgtta tatgttatat caagtacttt
    22621 tattttctgt cggcaagtat ataaaaagtc gatatacgaa ctgtatgatg gtggatataa
    22681 cgcctcaagc tctgccataa ttgattttgt caacttagga ctcgcaccat cagttgatac
    22741 actgatagtt agcttgtcgc ggtgtagtgc acttggaaat acaacattgc catttgatgc
    22801 atctccaaca ttattaaaaa gggcatgctc aggtaaggct ttttttaccg cttcattgac
    22861 acgtggctca ttggttgctg caataactag atacgcgtct acaatatcgc ttggttcaaa
    22921 ttctttttct ttccatatga cgacaccgtt atctaccata ttttgaagtt tttcagtgat
    22981 tgtcggactg atgaccgtca tatgttcaac gtattgattt aatgtttgtg cccgacgact
    23041 tgcaacgacg cctccaccaa ctatgacgac atttttattt gttaaatcta tcattaatgg
    23101 catattcatg ttgagcaaca tcctttatat ctttttacat cattacacct taaaatattt
    23161 aacaccaatc gctaataaac atcacgttga tagcgttgtt gttgtttcat ttgtcgcaat
    23221 aataactctg cttcttcttg agaaatatga cgttctttta ccaatacatc tttaatggct
    23281 tgatggacat ctttcgccat acatttttca tcgccacaaa tatagattgc tgcgccttgc
    23341 tcaatccatt cattgaaacg tttactttct tccattatac gatgctgtac atattctttg
    23401 tgttcttggt ctcttgaaaa tgctaaatct acgcgtgtta agtttccatt ttcaagccat
    23461 tcttctattt cttcttcata taaaaagtca gaactacggt gttgatcacc aaagaacaac
    23521 catgtttttc cagtcatacc aagttcttca cgttcttgta aatatgctct aaaaggagca
    23581 attccagtac ctggtccaat cataataacc ggtatatctt gcttcatcgg aaatttgaag
    23641 ttcggatttt tcttcaaata aattggtact atatcgcctg gtttaattcg ctcagcaaaa
    23701 tgaaccgagc atacaccttt tctctcacgt ccatgtgctt gataacgaac cgtaccaacg
    23761 gtaatatgca cttcatctgg cgttgccata aaactactag aaatcgaata ctctcttggt
    23821 ggtaattttc taaggatttg atacatattc tcaggttgta attctatagt tggaaaatct
    23881 gttattaaat ctataaagtc ccgatttata acatattcac gcgcccatga ctcatcttga
    23941 atacgttcag ataattcttc attgtcaaaa tagatatctg catttttcaa taatggcaat
    24001 gttaatttag taaattcaaa atgtgatgtt agtgcttcaa caataggaac tgtatcacca
    24061 tgatcattaa ttggcaccgg agattgcgga tcccaaccta acatggatat tagtttttca
    24121 accaattcag ggtcgttttg cggtaatgct actatacaat ctcctggttc atatgattca
    24181 ctaaaatcat caagtaaaaa ttctatatgt cgtgtttctt tatttgaatc ggtaccattt
    24241 aaattgatat tcgctaatac ttctgcttgg tatggatttg atttagaata tttcttttct
    24301 ttggcagact taattgattc acttatcacc gattcacttt gaataccttc tgatgtggta
    24361 tcaataatat taatgatgtc tgccatccac ttttctgcgt cttcttcata atcaatatca
    24421 caatctacac gcttacatat acgctcagcg cctagatttt ctagtaaaac atctacatct
    24481 ttaccggctt gacagaaaaa ttcatatgtt tgatcaccta aagctagtac tgaatatctc
    24541 acatgattta aattaggtgc gttatcgtct tctaaaaatt caaagaaatc ccatgcatta
    24601 tcaggcggtt ctccttcacc atgagtagac gtaataataa ataaatcttc taactgcgcg
    24661 atgtttgtcg tatcatattc atccattgac atcaaaacaa cttgatgtcc gatatcactt
    24721 aaacgttctg aaaaaatttc agctaaacgc atggcattac ctgattcaga accatatagc
    24781 actgtaacat gccgctgatt agcttcgata tgtggctctt tattttgtaa catatacgtt
    24841 gttgaagttg cttgttcttc tgatatttgt gattgttgcc acacttctgt ctctaattgt
    24901 tgttgattac tatcacttgt cgtatcgtta ctattagcta gtaaaaaacc actaagccac
    24961 tgttgttggc tctctgttaa tgtttgcagc agattgttaa tctctgtgac ttgtttttct
    25021 gtaaatggac tattagatgt atttaatttc aaatgttttc acctcaataa acttgaaata
    25081 gatgattgac tcttttttta tcgatagttt agtcaatttt ttaatatgta tggtataaat
    25141 taaaacaaac ttgagttgtg tctgatagtt atacttgcaa atatttacaa ccttcatgtt
    25201 gcttgaatga cattatcagt aattcagaca aaactttgat tatgtcgtgt cagttattaa
    25261 tgcatacagt aatgcatcgt tgttattttt tatttattta ctatttatta tgtaccaaat
    25321 cagcctttca gtaaaatagg tcaaattact gattttctaa atataaaatg cctcctaata
    25381 acatactatt agtacatcat taagaggctc ttgtgttatt tgcatactaa gcgctcaaat
    25441 ttaaatttaa gatgaagatt cttgcaataa tatttctata tttgtcgata tatccaatgg
    25501 atcttccatt ggcaagaatc gatttacaac atttccttgt cgatcgatta taaattttgt
    25561 gaaattccat tttattgggg acccaaagat tcctggttgt tcattcttta aatgcgtaaa
    25621 taacggatgt tcatcgttcc cgttcacaga tattttagct aacactggaa atgttacacc
    25681 aaatttctca cgactaattt tcaagatttc ttcattagaa cctggttgtc gattgtcaaa
    25741 attattattc ggaaaactca acactacaaa cccacgatcc ttatattttt gaaataaagt
    25801 ctctagtttt ttcaattgtt cgctatatat acattctgtt gcagtattaa caactaaaat
    25861 cactttacct ctaaatgctt ctaatttata agttaagcct ttataatcac ttacttcgat
    25921 atcatacaca tttctattat tcataagaca cccctacaca gcctttttta tattgaatat
    25981 gttcttttta gaatgttctg ataaaataag tgcgcgttta caccgtgaac acacattata
    26041 tagcgtgata catttttcga gcacacgata aataatgttc gagtttatgt tgttgctcaa
    26101 cctatccgat ttaccgtctt ttttcacctg tcttacttta ttaaaataat cacattgtat
    26161 agctgtatta gtccagctac aatatcttcc gttataaccc ctcattataa tcatccttat
    26221 tttctatttt taaaaagaca attagaccgc tctttaaact atagattaat acttaagtta
    26281 aactcataca tactgatacc atacgttaga tctaacaatt taaaattcgt tataactatg
    26341 gattaaagag ctgcccaact catataatcc ttaaaaactt cacatgtgat tgtttattaa
    26401 gccctccttt atcttattaa atatccttat aaccctttta aaattaaact gacacactca
    26461 tacattgtta cacgcgacga taaacgtcat attattgcat atcattaaaa caagtcactt
    26521 tgtatcgtgt gcgaccgaat acatattaaa tcatcaataa tgattattca attattaaaa
    26581 ataaaaacga acattctata tagagtttct acttattgtt ttttcagcat tgtctacatt
    26641 acttaacatt cgtgtttgta agtaatattg accgccaata tttagacac
//
LOCUS       DAXWFK010000016        26568 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.016, whole genome shotgun sequence.
ACCESSION   DAXWFK010000016 DAXWFK010000000
VERSION     DAXWFK010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 26568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26568
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.016"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..83)
                     /locus_tag="ACGEXS_000553"
     CDS             complement(<1..83)
                     /locus_tag="ACGEXS_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011600.1"
                     /translation="MLKSKFPSPLSTVDISFFEIIYIFSYQ"
     gene            complement(421..825)
                     /locus_tag="ACGEXS_000554"
     CDS             complement(421..825)
                     /locus_tag="ACGEXS_000554"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFF8011601.1"
                     /translation="MNTLNLDIVTPNGSVYNRDNVELVVMQTTAGEIGVMSGHIPTVA
                     ALKTGFVKVKFHDGTEYIAVSDGFVEVRKDKVSIIVQTAETAREIDVERAKLAKARAE
                     SHLENDDDNTDIHRAERALERANNRLRVAELK"
     gene            complement(845..2257)
                     /gene="atpD"
                     /locus_tag="ACGEXS_000555"
     CDS             complement(845..2257)
                     /gene="atpD"
                     /locus_tag="ACGEXS_000555"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFF8011602.1"
                     /translation="MGIGRVTQVMGPVIDVRFEHNEVPKINNALVIDVPKEEGTIQLT
                     LEVALQLGDDVVRTIAMDSTDGVQRGMDVKDTGKEISVPVGDETLGRVFNVLGETIDL
                     KEEISDSVRRDPIHRQAPAFDELSTEVQILETGIKVVDLLAPYIKGGKIGLFGGAGVG
                     KTVLIQELINNIAQEHGGISVFAGVGERTREGNDLYFEMSDSGVIKKTAMVFGQMNEP
                     PGARMRVALSGLTMAEYFRDEQGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTTKGSVTSIQAVFVPADDYTDPAPATAFAHLDATTNLERKLTE
                     MGIYPAVDPLASTSRALEPSIVGQEHYEVARDVQSTLQKYRELQDIIAILGMDELSDE
                     DKQTVERARRIQFFLSQNFHVAEQFTGQKGSYVPVKTTVANFKDILDGKYDHIPEDAF
                     RLVGSMDDVIAKAKDMGVEV"
     gene            complement(2279..3145)
                     /gene="atpG"
                     /locus_tag="ACGEXS_000556"
     CDS             complement(2279..3145)
                     /gene="atpG"
                     /locus_tag="ACGEXS_000556"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HFF8011603.1"
                     /translation="MASLKEIDTRIKSTKKMKQITKAMNMVSSSKLRRAEKNTKQFTP
                     YMDKMQDAITAVAGASSNTNHPMLRPRKITRSGYLVITSDKGLAGAYSANVLKKLITD
                     IEAKHQDSSEYSIVVLGQQGVDFLKNRGYDIEYSQVDVPDQPSFKSVQALANHAIDLY
                     SEEEIDELNIYYSHYVSVLENKPTSRQVLPLSQEDSSKGHGHLSSYEFEPDKESILSV
                     ILPQYVESLIYGTILDAKASEHATRMTAMKNATDNATELIDDLSLEYNRARQAEITQQ
                     ITEIVGGSAALE"
     gene            complement(3176..4684)
                     /gene="atpA"
                     /locus_tag="ACGEXS_000557"
     CDS             complement(3176..4684)
                     /gene="atpA"
                     /locus_tag="ACGEXS_000557"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFF8011604.1"
                     /translation="MAIKAEEISALLRSQIENYESEMSVTDVGTVLQIGDGIALIHGL
                     NDVMAGELVEFHNGVLGLAQNLEESNVGVVILGPYTGITEGDEVKRTGRIMEVPVGEE
                     LIGRVVNPLGQPIDGQGPINTTKTRPVEKKATGVMDRKSVDEPLQTGIKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQGTICIYVAIGQKDSTVRANVEKLRQAGAL
                     DYTIVVAASASEPSPLLYIAPYSGVTMGEEFMFNGKHVLIVYDDLTKQAAAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLNDDLGGGSITALPIIETQAGDISAYVPTNV
                     ISITDGQIFLQSDLFFSGVRPAINAGQSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     ESFAQFGSDLDEFTASKLERGKRTVEVLKQDQNKPLPVEHQVLIIYALTKGYLDDIPV
                     VDITRFEDELNHWAESNATELLNEIRETGGLPDAEKFDTAINEFKKSFSKSE"
     gene            complement(4706..5245)
                     /locus_tag="ACGEXS_000558"
     CDS             complement(4706..5245)
                     /locus_tag="ACGEXS_000558"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFF8011605.1"
                     /translation="MVKVANKYAKALFDVSLDTNNLETINEELTVINEAVKDKIEQLR
                     MVDSNPTQTAEQRRELINGVFTDINPYIKNMMYVLADNRHISLIADVFKAFQSLYNGH
                     YNQDFATIESTYELSQEELDKIVKLVTQQTKLSKVIVDTKINPDLIGGFRVKVGTTVL
                     DGSVRNDLVQLQRKFRRVN"
     gene            complement(5245..5766)
                     /locus_tag="ACGEXS_000559"
     CDS             complement(5245..5766)
                     /locus_tag="ACGEXS_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFF8011606.1"
                     /translation="MTETANLFVLGAAGGVEWGTVIVQVLTFIVLLALLKKFAWGPLK
                     DVMDKRERDINRDIDDAEQAKLNAQKLEEENKQKLKETQEEVQKILEDAKVQARQQQE
                     QIIHEANVRANGMIETAQSEINSQKERAIADINNQVSELSVLIASKVLRKEISEQDQK
                     ALVDKYLKEAGDK"
     gene            complement(5964..6176)
                     /gene="atpE"
                     /locus_tag="ACGEXS_000560"
     CDS             complement(5964..6176)
                     /gene="atpE"
                     /locus_tag="ACGEXS_000560"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFF8011607.1"
                     /translation="MNLIAAAIAIGLSALGAGIGNGLIVSRTVEGVARQPEARGQLMG
                     IMFIGVGLVEALPIIGVVIAFMTFAG"
     gene            complement(6219..6947)
                     /gene="atpB"
                     /locus_tag="ACGEXS_000561"
     CDS             complement(6219..6947)
                     /gene="atpB"
                     /locus_tag="ACGEXS_000561"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFF8011608.1"
                     /translation="MDHKSPLVSWNLFGFDIVFNLSSILMILVTAFLVFLLAIICTRN
                     LKKRPTGKQNFVEWIFDFVRGIIEGNMAWKKGGQFHFLAVTLILYIFIANMLGLPFSI
                     VTKDHTLWWKSPTADATVTLTLSTTIILLTHFYGIKMRGTKQYLKGYVQPFWPLAIIN
                     VFEEFTSTLTLGLRLYGNIFAGEILLTLLAGLFFNEPAWGWIISIPGLIVWQAFSIFV
                     GTIQAYIFIMLSMVYMSHKVADEH"
     gene            complement(6968..7420)
                     /locus_tag="ACGEXS_000562"
     CDS             complement(6968..7420)
                     /locus_tag="ACGEXS_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HFF8011609.1"
                     /translation="MNRIDTTNRLYYHLVCMSGTVFMIDISHTEAYKMSRFYNIFKQF
                     IQYYFYLIIILGGLYLYTHHAFILGLIIGVTGSAINTCIFECYLAKAKRPDTMHISTG
                     NMWRYLVAIIACMIWYLNKAHVSIIGIIIGLMISYVVVIIRPLLKVSK"
     gene            complement(7488..8615)
                     /gene="wecB"
                     /locus_tag="ACGEXS_000563"
     CDS             complement(7488..8615)
                     /gene="wecB"
                     /locus_tag="ACGEXS_000563"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFF8011610.1"
                     /translation="MKKIMTIFGTRPEAIKMAPLVKALEQEKMLEPIVVVTAQHREML
                     DSVLSTFEIKPKYDLNIMKSGQTLSEITSKSITQLEQVIQLEKPDMVLVHGDTMTTFA
                     GGLAAFYNQVPIGHVEAGLRSYDKYSPFPEEVNRQLVGVLADLHFAPTKNAASHLLSE
                     GKYSESVVVTGNTAIDAMKYTVDDNYKSNIMDKYHDKKFILMTAHRRENIGKPMENIF
                     KAVRRLIDEYTDLALVYPMHKNPKVREVAQKILGSHDRIELIEPLDVVDFHNFAKKSY
                     FILTDSGGIQEEAPSFNKPVLVLRSVTERPEGVEAGTLKVIGTNKQNVYQAAKELIDD
                     ERLYHQMSEASNPYGDGFASERIVNHIKYYLNLITEKPSDF"
     gene            complement(8639..9268)
                     /gene="upp"
                     /locus_tag="ACGEXS_000564"
     CDS             complement(8639..9268)
                     /gene="upp"
                     /locus_tag="ACGEXS_000564"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFF8011611.1"
                     /translation="MSKVHVFDHPLIQHKLSYIRDVNTGTKEFRELVDEVGMLMAYEV
                     TRDLELQDVDIETPVTKMTAKRLAGKKLAIVPILRAGLGMTDGILSLVPAARVGHIGL
                     YRDPETLKAVEYFAKLPQDITERQIIVVDPMLATGASAIEAITSLKKRGAKNIRFMCL
                     IAAPEGVEKMHEAHPDVDIYIAALDEKLNDKAYITPGLGDAGDRLFGTK"
     gene            complement(9296..10534)
                     /gene="glyA"
                     /locus_tag="ACGEXS_000565"
     CDS             complement(9296..10534)
                     /gene="glyA"
                     /locus_tag="ACGEXS_000565"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFF8011612.1"
                     /translation="MSYITKQDKVIAEAIEREFQRQNSNIELIASENFVSEAVMEAQG
                     SVLTNKYAEGYPGRRYYGGCEFVDVTESIAIDRAKALFGAEHVNVQPHSGSQANMAVY
                     LVALEMGDTVLGMNLSHGGHLTHGAPVNFSGKFYNFVEYGVDKDTERINYDEVRKLAL
                     EHKPKLIVAGASAYSRTIDFKKFKEIADEVNAKLMVDMAHIAGLVAAGLHPNPVEYAD
                     FVTTTTHKTLRGPRGGMILCKEEYKKDIDKTIFPGIQGGPLEHVIAAKAVAFGEALEN
                     NFKTYQQQVVKNAKVLAEALINEGFRIVSGGTDNHLVAVDVKGSIGLTGKEAEETLDS
                     VGITCNKNTIPFDQEKPFVTSGIRLGTPAATTRGFDEKAFEEVAKIISLALKNSKDEE
                     KLQQAKERVAKLTAEYPLYQ"
     gene            complement(10561..11085)
                     /locus_tag="ACGEXS_000566"
     CDS             complement(10561..11085)
                     /locus_tag="ACGEXS_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFF8011613.1"
                     /translation="MKDLTMLLDELKDMSFFNKGDICLIGCSTSEVIGEKIGTVGSME
                     VAETIFNALDVVSKETGVTFAFQGCEHINRAITIEKSQYNPLTMEEVSVVPDVHAGGS
                     LATYAFQHMKDPIVVEHITVPCGIDIGQTLIGMHIKHVCVPVRTSVKQVGQAIVTIAT
                     SRPKKIGGERAKYQ"
     gene            complement(11192..11611)
                     /locus_tag="ACGEXS_000567"
     CDS             complement(11192..11611)
                     /locus_tag="ACGEXS_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HFF8011614.1"
                     /translation="MKILFVCTGNTCRSPLAESIAKEVMPNHQFESRGIFAVNNQGVS
                     NYVEDLVEEHHLAETTLSQQFTEADLKADIILTMSYSHKELIEAHFGLQNHVFTLHEY
                     VKEAGEVIDPYGGTKEMYVHTYEELVSLILKLKDIIC"
     gene            complement(11608..12654)
                     /locus_tag="ACGEXS_000568"
     CDS             complement(11608..12654)
                     /locus_tag="ACGEXS_000568"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFF8011615.1"
                     /translation="MDTKIWDVREYNEDLQQYPKINEIKDIVLNGGLIGLPTETVYGL
                     AANATDEEAVAKIYEAKGRPSDNPLIVHIHSKGQLKDFTYTLDPRVEKLMQAFWPGPI
                     SFILPLKLGYLCRKVSGGLSSVAVRMPSHSVGRQLLQIINEPLAAPSANLSGRPSPTT
                     FNHVYQDLNGRIDGIVQAEQSEEGLESTVLDCTSFPYKIARPGSITAAMITEILPNSI
                     AHADYNDTEQPIAPGMKYKHYSPNTPLTIITDIESKIGNDGKDWSSIAFIVPSNKVAF
                     IPSEAQFIQLCQDDNDVKQASHNLYDVLHSLDENENISAAYIYGFELNDNTEAIMNRM
                     LKAAGNHIIKGCEL"
     gene            complement(12738..13574)
                     /gene="prmC"
                     /locus_tag="ACGEXS_000569"
     CDS             complement(12738..13574)
                     /gene="prmC"
                     /locus_tag="ACGEXS_000569"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFF8011616.1"
                     /translation="MVNYKEKLDEAIHLTQQKGFEQTRAEWLMLDVFQWTRTDFVVHM
                     HDDMPKAMIMKFDLALQRMLLGEPIQYIVGFASFYGRTFDVNSNCLIPRPETEEVMLH
                     FLQQLEDDATIVDIGTGSGVLAITLKCEKPDLNVIATDISLEAMNMARNNAEKHQSQI
                     QFLTGDALKPLINEGIKLNGLISNPPYIDEKDMVTMSPTVTRFEPHQALFADNHGYAI
                     YESIIEDLPHVMEKGSPVVFEIGYNQGEALKSIILNKFPDKKIDIIKDINGHDRIVSF
                     KW"
     gene            complement(13561..14637)
                     /gene="prfA"
                     /locus_tag="ACGEXS_000570"
     CDS             complement(13561..14637)
                     /gene="prfA"
                     /locus_tag="ACGEXS_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFF8011617.1"
                     /translation="MFDQLDIVEERYEQLNELLSDPDVVNDSDKLRKYSKEQADLQKT
                     VDVYRNYKAKKEELADIEEMLSETDDKEEVEMLKEESNGIKAELPNLEEELKILLIPK
                     DPNDDKDVIVEIRAAAGGDEAAIFAGDLMRMYSKYAESQGFKTEIVEASESDHGGYKE
                     ISFSVSGNGAYSKLKFENGAHRVQRVPETESGGRIHTSTATVAVLPEVEDVEIEIRNE
                     DLKIDTYRSSGAGGQHVNTTDSAVRITHLPTGVIATSSEKSQIQNREKAMKVLKARLY
                     DMKVQEEQQKYASQRKSAVGTGDRSERIRTYNYPQSRVTDHRIGLTLQKLGQIMEGHL
                     EEIIDALTLSEQTDKLKELNNGEL"
     gene            complement(14638..15237)
                     /locus_tag="ACGEXS_000571"
     CDS             complement(14638..15237)
                     /locus_tag="ACGEXS_000571"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFF8011618.1"
                     /translation="MYETYHSGWIECITGSMFSGKSEELIRRLRRGIYAKQKVVVFKP
                     AIDDRYHKEKVVSHNGNAIEAINISKASEIMTHDLTNVDVIGIDEVQFFDDEIVSIVE
                     KLSADGHRVIVAGLDMDFRGEPFEPMPKLMAVSEQVTKLQAVCAVCGSSSSRTQRLIN
                     GKPAKIDDPIILVGANESYEPRCRAHHIVAPSDNNKEEL"
     gene            complement(15584..15838)
                     /locus_tag="ACGEXS_000572"
     CDS             complement(15584..15838)
                     /locus_tag="ACGEXS_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFF8011619.1"
                     /translation="MKQGIHPEYHQVIFLDTTTNFKFLSGSTKTSSEMMEWEDGKEYP
                     VIRLDISSDSHPFYTGRQKFAAADGRVERFNKKFGLKSNN"
     gene            complement(15956..17272)
                     /gene="rho"
                     /locus_tag="ACGEXS_000573"
     CDS             complement(15956..17272)
                     /gene="rho"
                     /locus_tag="ACGEXS_000573"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFF8011620.1"
                     /translation="MPERERTSPQYESFHELYKNYTTKELTQKAKTLKLTNYSKLNKK
                     ELVLAIMEAQMEKDGNYYMEGILDDIQPDGYGFLRTVNYSKGEKDIYISASQIRRFEI
                     KRGDKVTGKVRKPKDNEKYYGLLQVDFVNDHNAEEVKKRPHFQALTPLYPDERIKLET
                     ETQNYSTRIMDLVTPIGLGQRGLIVAPPKAGKTSLLKEIANAISTNKPDAKLFILLVG
                     ERPEEVTDLERSVEAAEVVHSTFDEPPEHHVKVAELLLERAKRLVEIGEDVIILMDSI
                     TRLARAYNLVIPPSGRTLSGGLDPASLHKPKAFFGAARNIEAGGSLTILATALVDTGS
                     RMDDMIYEEFKGTGNMELHLDRKLSERRIFPAIDIGRSSTRKEELLISKSELDTLWQL
                     RNLFTDSTDFTERFIRKLKRSKNNEDFFKQLQKSAEESTKTGRPII"
     gene            complement(17519..18946)
                     /locus_tag="ACGEXS_000574"
     CDS             complement(17519..18946)
                     /locus_tag="ACGEXS_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFF8011621.1"
                     /translation="MRDYTKQYINGEWVESNSNETIEVINPATEEVIGKVAKGNKADV
                     DKAVEAADDVYLEFRHTSVKERQALLDKIVKEYENRKDDIVQAITDELGAPLSLSERV
                     HYQMGLNHFVAARDALDNYEFEERRGDDLVVKEAIGVSGLITPWNFPTNQTSLKLAAA
                     FAAGSPVVLKPSEETPFAAVILAEIFDKVGVPKGVFNLVNGDGAGVGNPLSEHPKVRM
                     MSFTGSGPTGSKIMEKAAKDFKKVSLELGGKSPYIVLDDVDIKEAAKATTGKVVNNTG
                     QVCTAGTRVLVPNKIKDAFLAELKEQFSQVRVGNPREDGTQVGPIISKKQFDQVQNYI
                     NKGIEEGAELFYGGPGKPEGLEKGYFARPTIFINVDNQMTIAQEEIFGPVMSVITYND
                     LDEAIQIANDTKYGLAGYVIGKDKETLHKVARSIEAGTVEINEAGRKPDLPFGGYKQS
                     GLGREWGDYGIEEFLEVKSIAGYFK"
     gene            complement(19184..19519)
                     /locus_tag="ACGEXS_000575"
     CDS             complement(19184..19519)
                     /locus_tag="ACGEXS_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFF8011622.1"
                     /translation="MEVCPYLEETFKILGRSWNGLIINYLSRCNDCSAHFSDMKRDLK
                     TITPRALSLKLSELAQWELVEKQIISTSPVQIIYVLTEKGKALAEALHPIEAWAQSYV
                     DLTDQRTAK"
     gene            complement(19608..20867)
                     /locus_tag="ACGEXS_000576"
     CDS             complement(19608..20867)
                     /locus_tag="ACGEXS_000576"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFF8011623.1"
                     /translation="MAQEVIKIRGGRTLNGEVNISGAKNSAVAIIPATLLAQGHVKLE
                     GLPQISDVKTLVSLLEDLNIKASLNGTELEVDTTEIQNAALPNNKVESLRASYYMMGA
                     MLGRFKKCVIGLPGGCPLGPRPIDQHIKGFKALGAEIDESSTTSMKIEAKELKGAHIF
                     LDMVSVGATINIMLAAVYATGQTVIENAAKEPEVVDVANFLTSMGANIKGAGTSTIKI
                     NGVKELHGSEYQVIPDRIEAGTYMCIAAACGENVILNNIVPKHVETLTAKFSELGVNV
                     DVRDERIRINNNAPYQFVDIKTLVYPGFATDLQQPITPLLFMANGPSFVTDTIYPERF
                     KHVEELKRMGANIEVDEGTATIKPSTLHGAEVYASDLRAGACLIIAGLIAEGVTTIYN
                     VKHIYRGYTDIVEHLKALGADIWTETV"
     gene            complement(21333..22193)
                     /gene="fdaB"
                     /locus_tag="ACGEXS_000577"
     CDS             complement(21333..22193)
                     /gene="fdaB"
                     /locus_tag="ACGEXS_000577"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002468309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IIb fructose-bisphosphate aldolase FdaB"
                     /protein_id="HFF8011624.1"
                     /translation="MPLVSMKEMLIDAKENGYAVGQYNINNLEFTQAILEASQEENAP
                     VILGVSEGAARYMSGFYTIVKMVEGLMHDLNITIPVAIHLDHGSSFEKCKEAIDAGFT
                     SVMIDASHSPFEENVATTKKVVEYAHEKGVSVEAELGTVGGQEDDVVADGIIYADPKE
                     CQELVEKTGIDALAPALGSVHGPYKGEPKLGFKEMEEIGLSTGLPLVLHGGTGIPTKD
                     IQKAIPFGTAKINVNTENQIASAKAVRDVLNNDKEVYDPRKYLGPAREAIKETVKGKI
                     KEFGTSNRAK"
     gene            22411..22932
                     /locus_tag="ACGEXS_000578"
     CDS             22411..22932
                     /locus_tag="ACGEXS_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2529 domain-containing protein"
                     /protein_id="HFF8011625.1"
                     /translation="MSKILNTQLIGIFNRLEKQSLEIQMAAQCLIQAIGGEGYVYVKG
                     YDDLQFFESFILHSDERLKSSRKLDAIKDFKEIDSTDRVLLFAPFYNDQVALDIQKLI
                     DLDIDVVLISNKPKTDDFPDHLVHFIDLSTPRPIVYTEDYDKIVQPHAMALNYVYYDI
                     YTQMIEMTRDLEL"
     gene            complement(23041..24651)
                     /locus_tag="ACGEXS_000579"
     CDS             complement(23041..24651)
                     /locus_tag="ACGEXS_000579"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFF8011626.1"
                     /translation="MTKFIFVTGGVVSSLGKGITASSLGRLLKDRGLNVTIQKFDPYL
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKFSNVTAGKVYSHVLKKE
                     RRGDYLGGTVQVIPHITNEIKERLLLAGESTNADVVITEIGGTTGDIESLPFIEAIRQ
                     IRSDLGRENVMYVHCTLLPYIKAAGEMKTKPTQHSVKELRGLGIQPDLIVVRTEYEMT
                     QDLKDKIALFCDINKESVIECRDADSLYEIPLQLSQQNMDDIVIKRLQLNAKYETQLD
                     EWKQLLDIVNNLDGKITIGLVGKYVSLQDAYLSVVESLKHAGYPFAKDIDIRWIDSSE
                     VTDENAAEYLADVDGILVPGGFGFRASEGKISAIKYARENNVPFFGICLGMQLATVEF
                     SRNVLGLEGAHSAELDPATPYPIIDLLPEQKDIEDLGGTLRLGLYPCSIKEGTLAQDV
                     YGKAEIEERHRHRYEFNNDYREQLEANGMVISGTSPDGRLVEMVEIPTNDFFIACQFH
                     PEFLSRPNRPHPIFKSFIEASLKYQQNK"
     gene            complement(24987..25517)
                     /gene="rpoE"
                     /locus_tag="ACGEXS_000580"
     CDS             complement(24987..25517)
                     /gene="rpoE"
                     /locus_tag="ACGEXS_000580"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFF8011627.1"
                     /translation="MKIQDYTKQMVDEKSFIDMAYTLLNDKGETMNLYDIIDEFRALG
                     DYEYEEIENRVVQFYTDLNTDGRFLNVGENLWGLRDWYSVDDIEEKIAPTIQKFDILD
                     ADDEEDQNLKLLGEDEMDDDDDIPAQTDDQEELNDPEDEQVEEEINHSDIVIEEDEDE
                     LDEDEEVFEDEEDFND"
     gene            complement(25629..26489)
                     /locus_tag="ACGEXS_000581"
     CDS             complement(25629..26489)
                     /locus_tag="ACGEXS_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8011628.1"
                     /translation="MAKELCFEGITLKAFDEQYRSAINDFDLNERQQIYSSLPKEVID
                     DAINDADRIANVAINDKNEVVGFFVLHRYYQHEGYDTPENVVYIRSLSINEKYQGFGY
                     GTKIMMSLPQYVQGVFPDFNHLYLVVDAENDNAWNLYERAGFMHTATKEEGPIGKERL
                     YYLDLDSKHVSSLKLEEESRSEVTNVHIINLMIDGQKVGFIALEQIGERMNIAAIEVD
                     KSYRFNGIGSSALRQLPTYLRKNYDNLNVITMILFGENNDFKPLCLNSNFVEIEQTDD
                     YVVFEKYLNY"
BASE COUNT         7973 a         5269 c         3855 g         9471 t
ORIGIN      
        1 tcttggtatg aaaaaatgta gataatttca aaaaatgaaa tgtcgactgt agatagtggt
       61 gaaggaaatt ttgattttag caatctatta gtgattgtaa aaaagtaaaa ctattcttgg
      121 gagtcggaca taaatcaatg tctagactct atagtgttgt atttggcagt agttgacggg
      181 gccccaacat aaagaaatac tttttcttta gaaattagta tttcttatac atgagtttta
      241 ctcatgtatt cctattttta aatacacatt agctgtgttt aatgataaag aaccactaca
      301 tcataaatct ttaatggttc tttatcaatt ccttcccccc tctcatgttt tgtatcttta
      361 aatcaatata attcaaaaaa gggtcgaaga tatgaaatca catcttcgac cctttattta
      421 ctattttaat tcagccacac gcaaacggtt atttgctctc tctaaagctc tttcggctct
      481 atgaatatca gtattgtcgt catcattttc caagtgagac tctgctcttg ctttggctaa
      541 tttagctctt tcaacatcaa tttctcttgc agtttctgca gtctgaacaa tgattgaaac
      601 tttatctttt ctaacttcaa caaagccatc gcttacagca atatattcag ttccatcgtg
      661 aaatttcact tttacaaagc ctgtttttaa agcagctaca gttggaatat gtccactcat
      721 gacacctatc tcaccagctg ttgtttgcat aacaacgagt tcaacattat cacgattgta
      781 aacagaacca ttaggagtga caatatctag gtttaatgta ttcattatcc attcctccta
      841 attgttatac ttcaacaccc atatctttag cttttgcaat aacatcatcc atgctaccaa
      901 ctaaacggaa tgcatcttct ggaatatggt catatttacc atctaagata tctttaaagt
      961 ttgcaactgt tgtcttaaca ggtacataag aacctttttg accagtaaat tgttccgcta
     1021 cgtggaagtt ttgagataag aagaattgaa ttctacgtgc gcgttcaact gtttgtttat
     1081 cttcatcaga taattcgtcc atacctaaga tagcaatgat atcttgtaat tcacggtatt
     1141 tttgaagtgt tgattgtaca tcacgagcta cttcataatg ttcttgacct acaattgatg
     1201 gttccaatgc tcttgatgta gacgctaatg gatccacggc tggataaata cccatttcag
     1261 ttaatttacg ttctaagttt gtagttgcat ctaaatgggc aaacgctgtc gcaggcgctg
     1321 ggtcagtata gtcatcggca ggtacgaata ccgcttgaat agaagtaact gatccttttg
     1381 ttgtagacgt aatacgttct tgtaattgtc ccatttcagt agcaagtgtt ggttggtaac
     1441 ctactgcaga aggcatacga cctaataatg cagatacctc agaaccagct tgtgtaaatc
     1501 tgaaaatgtt atcgatgaat aataatacgt cttgaccttg ttcgtcacgg aaatattcag
     1561 ccattgttaa accagataat gcaacacgca tacgtgcacc aggtggctca ttcatttgcc
     1621 cgaataccat ggctgttttc ttaattacac cactgtcact catttcgaag tataaatcgt
     1681 taccttcacg agtacgttca cctacaccgg cgaatacaga aataccaccg tgctcttgag
     1741 cgatgttgtt aattaattct tggattaata ctgttttacc tacaccggca ccaccgaaca
     1801 atccgatttt accaccttta atataaggtg ctagtaaatc tactacttta atacctgttt
     1861 ctaaaatttg aacttctgtt gaaagttcat cgaatgctgg tgcttgacga tggataggat
     1921 cgcggcgaac agaatcacta atttcttctt taaggtcaat tgtttcacct agtacattaa
     1981 atacacgacc taatgtttcg tcaccaacag gtacactaat ttctttgcct gtatctttta
     2041 catccatgcc tctttggaca ccatcagttg aatccatcgc aattgtacga acaacgtcgt
     2101 cacctaattg cagcgcaact tctaatgtta gttgtattgt accttcttct ttaggcacat
     2161 caataaccaa ggcgttatta attttaggaa cttcgttatg ttcaaatcga acatcaatta
     2221 caggacccat aacttgagtt acacggccaa ttcccatgct attttcctcc tttaaatatt
     2281 attcaagcgc tgcggaacca ccaacaattt cagtaatttg ttgcgtaatt tctgcttgtc
     2341 tcgctctgtt atattctaat gataagtcat caataagttc agttgcatta tcagtggcat
     2401 ttttcatcgc agtcatacgt gttgcatgct cacttgcttt tgcgtctaat attgttccgt
     2461 aaatcaaact ctcaacatat tgaggcaaga ttacacttaa gatagattct ttatctggct
     2521 caaattcata agaagacaaa tgaccatgcc ccttactaga atcctcttga gataatggta
     2581 atacttgtct agatgtaggc ttgttttcaa gaacgctgac ataatgacta tagtatatat
     2641 ttaattcatc aatttcttct tcactgtata agtctatagc atggttagct agtgcttgaa
     2701 cagatttgaa agaaggttga tcaggtacgt ctacttgaga atactcaatg tcataacctc
     2761 tattttttag gaaatcaaca ccttgttgcc ctaaaactac aatactgtat tcgctactat
     2821 cttgatgttt cgcttcaata tcagtaatca attttttaag cacgtttgca ctatatgcac
     2881 ctgctaaacc tttatcactc gtgataacta aatatccact tctagtaatt ttacgaggtc
     2941 ttagcattgg atggtttgta ttgctacttg cccctgcaac tgcagtaatt gcatcttgca
     3001 ttttatccat atatggtgtg aattgttttg tatttttttc agctctacga agttttgaac
     3061 ttgataccat gttcatcgct ttcgtaatct gcttcatttt tttggttgat tttattcgag
     3121 tatctatttc tttaagagaa gccactatct caccacctta tactaaactt gtttattatt
     3181 cagatttgct aaagcttttt ttgaattcgt taattgctgt gtcaaacttc tcagcatctg
     3241 gtaagccacc agtttctctg atttcattta acagttcagt agcatttgat tctgcccagt
     3301 ggtttaactc gtcttcaaaa cgtgtgatat ctacaactgg aatatcatct aaatatcctt
     3361 ttgttaatgc ataaataatc aacacttggt gttcgactgg taatggtttg ttttgatctt
     3421 gttttaagac ttcaacagta cgtttaccac gttctaattt acttgcagta aattcatcaa
     3481 ggtctgaacc gaattgtgca aatgattcaa gttctctgta tgacgctaag tcaagacgta
     3541 acgtaccagc aactttcttc attgctttaa tttgtgcaga tccaccaaca cgagatacag
     3601 attgtccggc attaatcgct ggtcttacac ctgagaagaa taaatcagat tgtaagaaga
     3661 tttgtccatc tgtaattgaa ataacgtttg ttggtacata agctgaaata tcaccagctt
     3721 gtgtttcgat aattggtaat gcagtaattg aaccgccacc taagtcatcg tttaattttg
     3781 ctgctctttc taataatcta ctatgtaagt agaatacgtc acctgggtat gcttcacgac
     3841 ctggaggtct acgtaataat aatgataact cacgataagc tgctgcttgt ttagttaaat
     3901 catcataaac gattaaaaca tgtttaccgt taaacatgaa ttcttcaccc attgttacac
     3961 ctgaatatgg tgcaatatat aataatggag aaggttcaga agctgatgct gctacaacaa
     4021 tagtgtagtc taaagcgcct gcttgtctta acttttcaac atttgctctt actgttgaat
     4081 ctttttgacc aatagcaaca tagatacaaa tcgtaccttg atctttttgg ttcaaaattg
     4141 tgtcaattgc aattgttgtt ttacctgttt gacggtcacc gatgattaac tcacgttgac
     4201 ctctaccaat tggtactaaa gcatcaattg ctttgatacc tgtttgtaat ggctcatcta
     4261 ctgatttacg atccattaca ccagtagctt ttttctctac tggacgtgtt ttagttgtgt
     4321 taatcggtcc ttgtccatca ataggttgtc ctaatggatt aacaactctt ccgattagtt
     4381 cttcacctac tggtacttcc atgatacgac cagtacgttt aacttcgtca ccttcagtaa
     4441 tacctgtgta tggtcctaaa ataaccacac ccacgtttga ctcttcaagg ttttgggcta
     4501 aaccaagtac gccgttatgg aattctacta gctcaccagc cataacgtca tttaatccgt
     4561 gaattaatgc aataccatca ccaatttgta atacagtacc tacatcagtt acggacattt
     4621 ctgactcata attttcaatt tgtgagcgaa gtaatgcact gatttcttca gcttttatgg
     4681 ccatctatgt cactcctctt tataattaat taactcttct aaattttctt tgtaattgga
     4741 caagatcatt tctaacacta ccatctaata cagttgtgcc gactttaact ctaaatccac
     4801 caattaaatc tggattaatt tttgtatcta caataacttt agataacttc gtttgttgag
     4861 ttactagttt gacaatctta tctaactctt cttgactcaa ttcatatgtt gactcaattg
     4921 ttgcaaaatc ttgattgtag tgtccgttat ataagctttg gaacgccttg aatacgtcag
     4981 ctattaatga gatatgtcta ttatctgcta acacgtacat catattttta atgtatggat
     5041 taatgtcagt aaatacacca ttaatcaact cacgacgttg ttctgctgtt tgagttggat
     5101 tactgtcaac cattctcaat tgctcaattt tatcttttac tgcttcattt ataactgtta
     5161 attcttcatt aatagtctct aaattatttg tatctaatga cacgtcaaat aatgctttag
     5221 catacttgtt agctactttt accattattt atcgcctgcc tcttttagat acttgtcaac
     5281 caatgctttt tggtcttgtt cagaaatttc ttttctaaga actttagaag caattaacac
     5341 tgatagttca gatacttgat tattaatatc tgcaatggca cgttcttttt ggctattgat
     5401 ttcactttgt gctgtttcaa tcataccgtt tgcacgtacg tttgcttcat gaataatttg
     5461 ttcttgctgt tgacgtgctt gaaccttagc atcttctaaa atcttttgaa cttcttcttg
     5521 tgtttcttta agtttttgtt tattttcttc ttcaagtttc tgtgcattta acttagcttg
     5581 ttctgcgtca tcgatatctc tgttaatatc tctttcacgt ttatccatta catctttcaa
     5641 tggaccccat gcgaactttt taagtaacgc aagtaacacg atgaaagtta ggacctgtac
     5701 aatcacagta ccccactcaa cgcctccagc tgcaccaaga acgaataagt tagctgtttc
     5761 agtcacgggt tcatgacact cctttcatta aaataatcgc tagtgttata tagaaaaaaa
     5821 cgacgaggtt aggacctact gaatttatgc gataggcatg gtgcgttcat atctcaacta
     5881 tttaataaag ttcaaagata ttactatata tgcatcatga attatgtcct atgcgctttg
     5941 tcccgacctc ttttatctgt taattatcca gcaaatgtca tgaatgcaat tactacaccg
     6001 atgataggta atgcctcaac taaacctaca ccaatgaaca tgatacccat taattgacca
     6061 cgtgcttctg gttgacgtgc tacaccttca actgttcttg aaacgattaa accgttaccg
     6121 atacctgctc ctaatgctga taaaccaatt gcgattgctg ctgcgattaa attcataatt
     6181 taatttcctc ctgtgattgt atataattat tgaaattttt agtgttcatc tgccacttta
     6241 tgtgacatat aaaccatcga aagcataata aagatatatg cttggattgt tcctacaaat
     6301 attgaaaatg cttgccaaac aattaatcct gggatactaa taatccaacc ccatgctggt
     6361 tcgttaaaga ataagccagc aagtaatgtt aatagtatct cacctgcaaa tatgttaccg
     6421 tacaaacgca gaccaagcgt taatgttgaa gtgaactctt caaaaacatt aataattgcc
     6481 aatggccaaa acggctgtac ataaccttta agatattgtt tcgtaccacg cattttaatt
     6541 ccataaaagt gagttaacag tattatcgtt gtagacaacg ttaaagtcac tgttgcatca
     6601 gctgtcggtg atttccacca caatgtgtga tctttcgtta ctatagaaaa cggaagacct
     6661 aacatattag ctataaaaat gtacagaatc agcgttactg ctaagaagtg gaattgacca
     6721 ccttttttcc aagccatgtt accttcaatg attcccctca cgaaatcaaa aatccattca
     6781 acgaaatttt gtttgccagt tggtcttttt ttcaaattac gcgtacaaat gatagcaagt
     6841 agaaaaacaa gaaacgccgt aacaagtatc atcaatatac ttgataaatt gaaaacgata
     6901 tcaaaaccga ataaattcca actcacgagc ggggatttgt gatccataat ctcacctctt
     6961 tcttaattta tttgctcacc tttagtaaag gacgtatgat aactacaaca tatgaaatca
     7021 ttaaaccaat aattataccg atgatactta catgcgcttt attaaggtac caaatcatac
     7081 aggcaataat tgcaactaag tatcgccaca tatttccagt tgaaatatgc atagtgtctg
     7141 gtcttttagc tttagctaaa tagcattcaa aaatacatgt gttgattgca gaaccagtaa
     7201 caccaatgat taatcctaag ataaatgcat ggtgtgtata aaggtataat cctcccaata
     7261 ttattattag ataaaaataa tattgaatga actgtttaaa aatgttgtag aaacgactca
     7321 ttttatatgc ctccgtgtga ctgatatcaa tcatgaaaac cgttccactc atacaaacta
     7381 aatgataata taggcgattt gtggtgtcaa ttctattcaa attgttgtaa aaaaataagc
     7441 atagttagac acaatttaga cacaattaga caaaaagtct attttcctca gaaatcgctt
     7501 ggtttttcag tgattaaatt caaataatac ttaatatgat taactattct ttctgaagca
     7561 aatccatcac cgtaaggatt agatgcttca gacatttgat gatataatct ctcatcatcg
     7621 attaattctt ttgcagcttg atacacattt tgcttatttg ttccaatgac ttttaatgtg
     7681 ccagcttcaa caccttcagg acgttctgta acacttcgca aaactaaaac tggcttatta
     7741 aatgatggcg cttcttcctg aattccacct gaatctgtca aaataaaata agatttttta
     7801 gcaaaattat ggaaatctac tacgtccaaa ggttcaatca attcaattct gtcatgacta
     7861 cctaaaatct tttgagccac ctctcgaact ttcgggtttt tatgcattgg atataccagt
     7921 gctaaatcag tatactcatc tattaagcgt ctaaccgctt taaatatatt ttccatgggt
     7981 ttcccgatat tttctcgtcg gtgtgctgtc ataagaatga attttttgtc atggtattta
     8041 tccatgatgt tagatttata attgtcatca actgtatatt tcatagcatc aatcgcagta
     8101 ttaccagtga caacaacact ttctgaatat ttcccttcac ttaacaaatg cgatgcagca
     8161 tttttagtag gtgcaaaatg taagtcagct aatacaccaa ctaattgtct attcacctct
     8221 tctggaaaag gtgaatattt atcataactt ctaagccctg cttcaacgtg tccaatcggc
     8281 acttggttat aaaatgccgc taaaccacct gcaaatgtcg tcatcgtatc accatgtaca
     8341 agtaccatgt ctggtttttc taattgaatc acttgttcta attgagtgat tgatttagaa
     8401 gttatctcag aaagtgtctg tcctgatttc ataatattca aatcgtattt tggtttgatt
     8461 tcaaaggtac ttaatactga atcaagcatt tctctatgct gtgctgtaac aacaacaatt
     8521 ggctcgagca ttttttcttg ttccaaagct ttaactaaag gagccatctt tatggcttcg
     8581 ggtcttgttc caaatatggt cataatcttt ttcatcaaac tacttatctc cgattcttct
     8641 atttagtacc aaacaatcta tctccagcgt cgcctaaccc tggtgtgata tatgctttgt
     8701 catttagctt ttcatcaagt gcagcaatat aaatatctac atctggatgt gcttcatgca
     8761 tcttttctac gccttctggt gctgcaatta aacacatgaa gcgaatattt ttagcgccac
     8821 gtttcttcaa tgaagtaata gcttcaattg ctgatgcgcc tgttgctaac ataggatcaa
     8881 caacaatgat ttgtctttca gtaatatctt gaggtaactt agcaaaatac tctacagcct
     8941 ttaatgtttc gggatctcga tataaaccga tatgtccaac tctggctgca ggtactaaac
     9001 ttaaaatacc atcagtcata cctaaaccag ctcttaaaat tggaacgata gctaattttt
     9061 taccagctaa tcgtttagcc gtcattttag ttacaggcgt ttcaatatca acatcctgaa
     9121 gctctaagtc tctagttact tcatatgcca tcaacatacc aacttcgtct acaagttctc
     9181 taaattcttt agtacctgta tttacatctc taatatagct tagtttgtgt tgaattaatg
     9241 gatgatcgaa aacgtgtact ttactcataa aaattactcc tatctttgtg tatgtttatt
     9301 gatatagagg atattcagct gttaatttcg caacgcgttc tttagcttgt tgtaattttt
     9361 cttcatcttt actatttttc aatgctaaac tgatgatttt tgcaacttcc tcaaaagctt
     9421 tttcatcaaa tccacgcgtt gttgcagcag gtgtacctaa acgtatacca ctcgttacaa
     9481 aaggtttttc ttgatcgaac ggaatggtat ttttgttaca tgtgatacca actgaatcta
     9541 aagtctcttc agcttcttta ccagtaagtc ctatagaccc ttttacatca acagctacta
     9601 agtgattatc tgtaccgcca gaaacaattc taaatccttc attaattaat gcttctgcaa
     9661 gaacttttgc gtttttaacc acttgttgtt gatacgtttt gaaattattt tctaacgctt
     9721 ctccaaaagc aactgctttt gctgcaataa catgctcaag aggtccacct tgaataccag
     9781 ggaaaattgt tttatctatg tcttttttat attcttcctt acataaaatc ataccaccac
     9841 gtggtccgcg taatgttttg tgtgttgtag ttgttacaaa atcagcatat tctactggat
     9901 ttggatgtaa acctgccgct actaatcctg caatatgtgc catgtctacc attaacttag
     9961 cgtttacttc atctgcgatt tctttaaact ttttgaagtc aattgttctt gaatatgctg
    10021 atgctcctgc cacaataagc ttaggcttat gctctaacgc taatttacga acttcatcat
    10081 aattgattcg ttctgtgtct ttatctactc catattcaac gaaattgtag aatttaccac
    10141 taaaattaac aggcgctcca tgtgtcaagt gaccaccatg actcaaattc atacctaaaa
    10201 ctgtgtcgcc catttctaat gcaactaagt aaacagccat gttcgcttgt gaacctgaat
    10261 gtggttgaac attgacatgt tcagctccaa acaatgcttt agcacgatca attgcgatgc
    10321 tttcagtaac atctacaaac tcacagccac cataatatcg gcgtcctgga tagccttcag
    10381 catacttatt agtcaacact gaaccttgtg cttccataac cgcttccgat acaaaatttt
    10441 ccgatgcgat taactctatg ttgctatttt gtctctgaaa ttctctctcg attgcttctg
    10501 cgataacttt atcttgcttg gtgatataag acataaaatc tccccttctt tcaaaaaaac
    10561 ttattggtat ttagcacgtt cgccaccaat ctttttcggc ctagatgtgg caatagttac
    10621 aattgcctgt cctacttgct ttactgaggt ccttacaggt acacatacat gtttaatatg
    10681 catgcctatt aacgtttgac caatatcaat tccacaagga acagtaatat gttcgaccac
    10741 gatcggatcc ttcatatgct gaaaagcgta tgttgccaaa ctccctccag catgtacatc
    10801 tggaacgacg gaaacttctt ccattgttaa tggattatac tgagattttt ctattgttat
    10861 cgctctgttg atatgttcac atccttgaaa agcaaaagta acgcctgtct ctttactcac
    10921 aacatctaat gcattaaaaa tagtttctgc aacttccatc gaaccgacag tccctatttt
    10981 ttcgccaatg acttccgatg ttgaacatcc aattaaacat atatctcctt tattaaaaaa
    11041 ggacatatct tttaattcgt ctaataacat tgtcaaatct ttcataaaag cccacccttc
    11101 ctaaaaataa aaaaggaata tagcaaagtg ctacactcct ctattataac ttatttaact
    11161 gttaacatat actaattata cagaattcct actagcaaat aatatctttt aattttaaaa
    11221 ttaaacttac aagttcttca taggtatgta catacatttc ttttgttcca ccgtatggat
    11281 ctataacttc tcctgcttct tttacatatt catgcaatgt gaaaacatga ttttgcaaac
    11341 caaagtgtgc ctctattaat tctttgtgcg aatacgacat cgtcaaaata atatctgctt
    11401 tcaaatctgc ttcagtaaat tgttgcgata aggtcgtttc agctaaatga tgttcttcaa
    11461 ctaagtcttc aacataattc gaaacacctt gattgttcac agcgaatata cctcttgatt
    11521 caaattgatg atttggcata acctcttttg caatactttc cgctaatggg ctacgacatg
    11581 tgttacctgt acaaacgaat aaaatcttca tagttcacat cctttaataa tgtgattacc
    11641 tgcagctttt aacatgcgat tcataattgc ttctgtatta tcattcagct caaagccgta
    11701 tatatacgcc gctgaaatat tttcattttc atcaagtgaa tgtaacacat cataaagatt
    11761 atgacttgct tgtttaacat cattgtcatc ctgacataat tgaatgaatt gcgcttcact
    11821 tggtataaac gccaccttat tactcggcac aataaaagct atagaagacc aatctttacc
    11881 gtcatttcca attttgctct caatatctgt aataattgta agtggtgtat tgggtgagta
    11941 atgcttatac ttcatacctg gtgcaattgg ctgttcagta tcattataat cagcatgggc
    12001 gatactattc ggaagtattt ctgtaatcat tgctgctgtt atagaaccag gtcttgcaat
    12061 tttataagga aaagatgtgc aatctaaaac cgtactttct aatccttctt cactttgttc
    12121 agcttgaaca ataccatcga tacggccatt caaatcttga tatacatgat tgaaagttgt
    12181 tggtgaaggt ctaccactta aattagcact tggagcagct agaggttcat ttatgatttg
    12241 taataattgt ctacctacag aatggcttgg cattctaaca gcaactgatg ataaacctcc
    12301 agaaactttt cgacatagat agcctagctt taacggcaat ataaacgaaa tagggcccgg
    12361 ccagaatgcc tgcattaact tttctacgcg tggatccaaa gtatatgtaa aatcttttaa
    12421 ttgaccttta ctgtgtatat gaacaataag cggattgtca gatggacggc ctttagcttc
    12481 atatatttta gctacagctt cttcatctgt cgcatttgct gcaagtccat aaactgtttc
    12541 agttggtaaa cctattaaac caccgtttaa aacaatgtct tttatttcat taattttagg
    12601 atattgctgt aaatcttcat tatattctct aacatcccaa attttagtat ccaacttaat
    12661 cacgcctttc ttatttatca taatataaag caaaaagcta tgcacttaac taatcatagc
    12721 aaaggcataa cttctaatta ccatttaaat gagacgattc gatcgtggcc atttatatct
    12781 ttaataatgt cgattttttt gtcaggaaat ttatttaaaa ttattgattt aagtgcctca
    12841 ccttgattgt aaccaatttc aaaaacaact gggctgcctt tttccataac gtgaggtaaa
    12901 tcttcaatga ttgattcata aatagcatat ccatggttat ctgcaaacaa tgcctgatgt
    12961 ggttcgaatc tcgtaaccgt tggagacatc gtaaccatat ctttttcatc tatatatggt
    13021 ggattagata tcaagccgtt caacttgata ccttcattaa ttaagggctt taatgcatcc
    13081 cctgttaaaa attgtatttg tgattgatgc ttctcagcat tattacgagc catattcatt
    13141 gcttcaagtg aaatatcagt agcaataaca tttaaatccg gcttttcaca tttcaaagta
    13201 attgcaagta caccactacc cgttccgata tctacgattg ttgcatcatc ttctaactgt
    13261 tgtaagaaat gcaacattac ttcttcagtt tcaggtcttg gtatcaaaca atttgagttt
    13321 acatcaaacg ttctaccata aaatgaggca aagccaacta tatactgtat aggctctcct
    13381 aataacatac gttgtaatgc taagtcgaac ttcataatca tcgctttcgg catatcatca
    13441 tgcatgtgga ctacaaagtc cgtacgcgtc cattgaaata catctaacat taaccattca
    13501 gctcgtgttt gttcaaaccc tttttgttgt gttaaatgaa ttgcttcatc taacttttct
    13561 ttataattca ccattattaa gttctttcaa tttatctgtc tgctctgata aagtcagtgc
    13621 atctataatt tcttctaaat ggccttccat aatttgccct aatttttgaa gcgttagacc
    13681 tatacgatgg tctgttacac ggctttgtgg ataattataa gttcgaatac gttctgaacg
    13741 atcaccagta ccgactgctg atttacgttg tgacgcatac ttttgttgtt cttcttgaac
    13801 tttcatatcg tataaacgtg cttttaacac tttcattgct ttttcacggt tttgaatttg
    13861 agacttctca gaagatgttg caatgacacc agttggtaaa tgggtaatac gtactgcaga
    13921 gtcagttgtg tttacgtgct gaccacctgc accacttgaa cgatacgtgt cgatttttaa
    13981 atcttcattt ctaatttcaa tttctacatc ttcaacttct ggtaaaactg ccactgtagc
    14041 tgttgaagta tgaatacgtc cacctgattc tgtttcaggc acacgttgaa cgcggtgcgc
    14101 accattttca aatttcaatt tactatacgc gccattacca gaaactgaga aactaatttc
    14161 tttgtaacca ccatggtcac tttcagacgc ttctactatt tcagttttga atccttgtga
    14221 ttcagcatac tttgaataca tacgcattaa atcaccagca aaaatcgcag cctcatcacc
    14281 acctgctgct gctcttattt ctacaataac gtctttgtca tcattaggat ctttaggaat
    14341 caataatatt ttaagctctt cttcaagatt tggaagttca gctttaatac cattactctc
    14401 ctcttttaac atttctactt cttctttatc atcagtctca cttaacattt cttcaatatc
    14461 agctaattct tcttttttag ctttatagtt acgataaaca tctacagttt tttgtaaatc
    14521 agcttgctct ttagaatatt tacgtaattt atctgaatca tttacaacat ctgggtcact
    14581 taacagttca tttaactgtt cgtatctttc ttctacaata tctaattgat caaacactta
    14641 taattcctcc ttattattat cactaggtgc tacgatatgg tgcgcgcgac aacgtggctc
    14701 ataactttca ttggcaccta ctaagataat cggatcatcg attttagctg gtttaccatt
    14761 tattaatcgt tgcgttctac tagatgaaga accacaaaca gcacaaactg cttgaagttt
    14821 cgttacttgt tcactgacag ccatcaattt aggcattggt tcgaacggtt cgcccctaaa
    14881 atccatatct aatccagcaa caataacacg gtgtccatct gctgatagtt tttctactat
    14941 acttacaatt tcatcgtcaa aaaattgcac ttcgtctatt cctataacat caacattagt
    15001 taagtcgtgc gtcataattt cacttgcttt agaaatatta atcgcttcaa tggcattacc
    15061 attatgagag accacttttt ctttatgata tcgatcatca atcgccggtt taaatacaac
    15121 gactttttgt ttagcgtata taccccttct tagacgtctt attagttctt cggatttacc
    15181 gctaaacata ctacctgtaa tacattctat ccaaccggaa tggtaagttt catacattga
    15241 gagttccacc tttttcaaaa cataatcgct ttattatatc atatttcaaa tattcataaa
    15301 tgtctttttc ataattatat cgatattgta catgaacaat tattttataa atttgtcttg
    15361 catatttata tacagctatt ctaatttgtt gcttcttatt cgtgcgatgt ttctactcaa
    15421 tacaaacttt aataaagggg gaaatttatc attatgtatt aacaacatgg tattgagtca
    15481 cttctttgaa cacagtcgta agtatatacc cttactacgc ttataaataa aaaagctgtc
    15541 tacaattcca ttataggatg cagacagctt aacgattact atattagttg tttgatttaa
    15601 gaccaaactt tttgttgaaa cgttccacac gtccatccgc agcagcgaat ttttgacgtc
    15661 cagtgtagaa tggatgtgaa tcagatgaaa tatctaaacg aataactggg tattcttttc
    15721 catcttccca ctccatcatt tctgaagatg tttttgtaga accgcttaag aatttgaagt
    15781 ttgtagtagt atctaaaaag attacttggt gatactcagg atgaattcct tgtttcatta
    15841 ttttcagctc ctttgccctg aaccatctgg aacagagtta tttgtgagtt tttacccaat
    15901 actgtgtaat tataaaggta attaattcaa aacgcaagcc cctatataat gtttattaaa
    15961 ttataggtcg acccgtttta gtactttctt ctgcagactt ttgtagctgc ttgaagaaat
    16021 cttcattatt cttagacctt ttaagtttgc gaataaatct ttcagtaaag tcagttgagt
    16081 cagtgaatag atttcttaat tgccataatg tgtctaattc agatttactt atcaacaatt
    16141 cttctttacg cgttgaactt ctgccaatat caattgcagg gaagatacga cgttcagaca
    16201 atttacgatc taaatgtaac tccatgttac ctgttccttt aaattcttcg taaatcatat
    16261 cgtccatacg tgaacccgta tcaactaatg cagttgcaag tattgttaaa cttccacccg
    16321 cttcaatatt tctcgctgca ccgaagaatg cttttggttt gtgtaaagat gcaggatcta
    16381 aaccacctga taatgtacga ccacttggtg gaataactaa gttataagcg cgtgctaatc
    16441 tcgttataga atccattaaa ataatgacat cttccccaat ttctactaaa cgctttgcac
    16501 gttcaagtaa taattcagct actttaacat ggtgttctgg tggttcgtca aacgttgaat
    16561 gaacgacttc agcagcttct actgagcgtt ctaaatctgt tacctcttca ggacgctcgc
    16621 caactaacaa aataaatagc tttgcatctg gtttgttcgt actgattgca ttcgctattt
    16681 cttttaataa cgatgtttta cctgctttag gtggcgccac tattaaacca cgttgaccta
    16741 aaccaatcgg tgttactaaa tccatgatgc gcgttgaata attttgtgtt tctgtctcta
    16801 atttaatacg ctcatctgga taaagtggtg tcaaagcttg gaaatgcgga cgtttcttca
    16861 cttcttctgc gttatggtca ttgacaaagt caacttgtaa taagccataa tatttttcgt
    16921 tatctttagg ttttctaact ttcccagtta ctttatcccc acgtttaatt tcaaaacgac
    16981 gaatttggct agcagatata taaatatctt tttccccttt agaatagttc actgttctta
    17041 aaaaaccata accatctggt tgtatatcat ctaagatacc ttccatataa tagttaccat
    17101 ctttttccat ttgtgcttcc ataatagcta gaacaagttc ttttttattt aatttactat
    17161 agttcgtcaa cttaagagtt ttagcttttt gagtgagttc cttggtagta tagttcttgt
    17221 acaattcgtg gaatgattca tactgaggag atgtacgttc tctttcaggc atatagttta
    17281 cacccatttc attatttttt agttagtaag taatagatag gcatgacaat gcataaataa
    17341 ctgtgctagc acaatcccat tatgctaatc cccaaccttt taaatacatg aataactata
    17401 gcaaattttt attttaatta caaaatctat ttataaaaat tgcaaaatta aaacagtaca
    17461 caaaacttaa ttagtcatgt gcactgtttt attgcgattt ttaattcaaa aatcaaattt
    17521 atttaaaata tccagctata gatttcactt ctaagaactc ttcaatacca taatcgcccc
    17581 attcacgacc taaaccagat tgtttatagc caccaaatgg taaatctggc tttctacctg
    17641 cttcgtttat ttctactgta cctgcttcaa tagaacgagc tactttatgc aatgtttctt
    17701 tgtccttacc aataacatat cctgccaaac catattttgt atcatttgca atttgaatcg
    17761 cttcatctaa atcgttataa gtgataactg acattactgg cccaaaaatt tcttcttgtg
    17821 ctatcgtcat ttgattatct acattaataa aaattgtcgg acgtgcaaag tatccttttt
    17881 caagtccttc tggtttacca ggaccaccat aaaataattc agcaccttct tcaatacctt
    17941 tattaatata attttgtact tgatcaaatt gttttttact aatgataggg cctacttgtg
    18001 taccatcttc tcttggatta ccgacacgca cttggctaaa ttgttctttt aattcagcta
    18061 agaatgcatc tttaattttg ttaggcacta aaacacgtgt accagctgta catacttgac
    18121 cagtattatt aacaactttg cctgttgttg ctttagccgc ttctttaata tctacgtcat
    18181 ctaggacgat atatggtgat ttgccaccaa gctctaatga taccttttta aaatctttag
    18241 cggctttttc cataatttta gaaccagtag ggcctgatcc tgtaaatgac atcatgcgta
    18301 ctttaggatg ttcagataaa ggattcccaa caccagcacc atcaccatta acaaggttaa
    18361 atacaccttt aggaacaccg actttatcaa aaatctcagc taaaataaca gctgcaaatg
    18421 gtgtttcttc agatggttta agtacaactg gactaccagc cgcaaatgct gctgctaatt
    18481 ttaatgatgt ttggtttgta gggaagttcc acggtgtaat taatccagat acaccgattg
    18541 cttctttaac aactaaatca tctccgcggc gttcttcaaa ttcgtagtta tctaatgcgt
    18601 ctctcgctgc aacaaaatgg tttagtccca tttgataatg gacacgctca gataatgata
    18661 aaggagcacc taattcatcc gtaatagctt gtacaatatc gtcttttctg ttttcatatt
    18721 cttttacaat tttatctaat aacgcttgtc tttctttcac agatgtatga cggaactcta
    18781 aataaacatc gtctgccgcc tcgacggctt tatcaacatc agctttatta cctttagcaa
    18841 ctttcccgat tacttcttcg gttgctggat ttataacttc tatcgtttca ttactattac
    18901 tttctaccca ttcgccatta atgtattgct ttgtgtagtc tctcattatt ctttcactcc
    18961 tcaagaatga tttatatatt atttatccgt aatgcatacg atataaacat tacattccat
    19021 tatgatacgc tcccttttaa tatgttcaaa ataaaatgct ttaaaatttt gctgcatcta
    19081 tttttttata tttttaaata aaaataacta ttcccacatc atcgcaaata atgcgaagac
    19141 ttagaaatag ttataatcaa tgctatgttt aaacgtcatg taattattta gcagtacgtt
    19201 gatctgttaa atcgacatat gattgcgccc atgcttcaat tggatgtaaa gcctctgcta
    19261 acgctttacc tttttcagtt agcacataaa taatttgtac tggactcgta gaaatgattt
    19321 gcttctcaac taactcccat tgtgcaagct ctgacaactt aagacttaaa gcacgtggtg
    19381 ttattgtttt caaatctctt ttcatatcgg aaaagtgtgc tgaacagtca ttacatcttg
    19441 agagataatt aatgattaat ccattccaac ttctaccaag tattttaaaa gtttcttcga
    19501 gatacggaca tacttccatc atcttcacct ctcatcaata aatttagaat gtaaacgaac
    19561 accatttttt aatcagtata attattatac cacaaaacaa gataaatcta tacagtttcc
    19621 gtccaaatat ctgcacctaa agcttttaag tgttctacaa tatctgtata acctctataa
    19681 atatgtttaa cattgtaaat tgtagttaca ccctcagcaa ttaaaccagc aataattaaa
    19741 caagctcctg ctcttaaatc gctagcataa acttcagcac catgtaatgt tgatggtttt
    19801 atcgttgctg tgccttcgtc aacttcaata tttgcaccca tgcgctttaa ttcttcaaca
    19861 tgtttaaaac gctccggata aatcgtatca gttacaaatg aaggaccatt tgccataaat
    19921 aataatggtg taataggctg ttgcaaatca gtagcaaaac ctggatatac tagtgtttta
    19981 atatcaacaa attgatatgg cgcattatta ttgatgcgaa ttctttcgtc tcttacatca
    20041 acattcacac ctaattcact aaatttagca gttaatgttt ctacatgttt cggaacaata
    20101 ttatttaata taacattttc tccacatgct gcagcgatac acatatatgt gcctgcttca
    20161 attctatcag gtataacttg atactcagaa ccatgtaatt ctttgacgcc attgatttta
    20221 attgttgatg tacccgctcc cttaatatta gctcccatac ttgttaagaa gttagcaaca
    20281 tcaactactt ccggttcttt agcagcattt tcaattacag tttgtcctgt tgcataaact
    20341 gcagctagca taatgttaat tgttgcacct acgctaacca tatctagaaa aatatgtgca
    20401 ccttttaact ctttagcttc gattttcatt gatgtcgtac ttgattcatc aatttcagca
    20461 cctaaagctt taaatccttt aatatgttga tcaattggac gtggcccaag tggacatcct
    20521 cccggtaaac caatcacaca ttttttaaat ctacctaaca tggcacccat catatagtat
    20581 gaagcacgta atgattcaac tttattattt ggtaatgcag cattttgtat ttcagttgta
    20641 tcaacttcta attccgtacc atttagtgat gccttaatat ttaaatcttc taataaactc
    20701 actaaagttt taacatcaga gatttgcggt aacccttcta atttcacatg tccttgagct
    20761 aataatgttg caggaatgat agctacagca ctgtttttcg cgccactgat attaacttcc
    20821 ccatttagtg tgcgtccacc tcttattttt attacctctt gagccatatc atttatctcc
    20881 tttgtccatt actttttaat tttttgcatt attagcatta taaattaatt ttactttatt
    20941 tgtaaaatag tttattttaa tcgttaaaat cttgtctttc tattttcgat tgattaataa
    21001 caaatcattc acctttacta atatatgctg ttcttcccca cttgaccatg gtctaaagtc
    21061 tgattattca actaaatatt gacgttgttt tgacaattat ttagttttaa ttctacggtt
    21121 ttcacgtaaa cttttttcat aaattgctca ttttccttat aattatatca caatcaattt
    21181 tgatatattt taccattttt acgctttcat tttaaaatca aactgtttta tttattactt
    21241 atacgacata aaaaagaagc aaacaccatt ttgtctgctt cttttaaaat taatatccct
    21301 acgttattaa taacttaaag actaaatatt aattatttag cgcggttaga agtaccgaac
    21361 tctttaattt tacctttaac tgtttctttg atggcttcac gtgcaggtcc taagtattta
    21421 cgaggatcgt aaacttcttt gtcgttattt aaaacgtcac gaactgcttt tgctgaagcg
    21481 atttggtttt cagtgtttac gttaatttta gctgtaccaa atggaattgc tttttggata
    21541 tctttagtcg ggataccagt accaccgtgt aatactaatg gtaaacctgt agataaaccg
    21601 atttcttcca tttctttaaa tcctaatttt ggttcacctt tgtatggacc atgaactgaa
    21661 cctaatgctg gcgctaatgc atcaatacca gttttttcaa ctagttcttg acattcttta
    21721 ggatcagcat aaatgatgcc gtctgctaca acatcatctt cttgtccacc aacagtacct
    21781 aattcagctt ctacagaaac acctttttca tgagcgtatt caacaacttt tttagttgtt
    21841 gctacgtttt cttcgaatgg gctgtgtgaa gcatcgatca ttactgatgt gaaaccagca
    21901 tcgatagctt ctttacattt ttcaaagctt gaaccatggt ctaaatggat tgctacagga
    21961 atagtgatgt ttaagtcatg cattaaccct tcaaccattt taacaattgt gtagaaaccg
    22021 ctcatgtaac gagcagcacc ttcagaaaca cctaaaatta caggtgcatt ttcttcttgt
    22081 gacgcttcta aaattgcttg agtgaattct aggttattaa tattgtattg acctaccgca
    22141 taaccatttt cttttgcatc aattaacatt tctttcattg aaactaaagg catgaaagtt
    22201 cctccttgag tgcttacgca cgtatttttc aaagtaatta tatcaaaaaa atagcgtcaa
    22261 tgcattgaaa tactgcagat taaatgtgta ttttttgaaa aatgcgatag ttatactaaa
    22321 tgcaaacgta tacattttta tacaatttaa gccgttgcat aatatgtagt cattaattac
    22381 aatattttat aagtagattg gaggataaag atgtcaaaaa tcttaaacac acaactaata
    22441 ggtatcttta ataggctaga aaaacaatct ttagaaattc aaatggccgc ccaatgttta
    22501 attcaagcaa ttggtggcga aggttatgtt tatgtaaaag gttatgatga tttacagttt
    22561 ttcgaaagtt tcattctaca tagtgatgag cgtttaaaat ctagccgaaa acttgacgct
    22621 attaaagact ttaaagaaat tgactcaact gatcgtgtat tattattcgc accattttat
    22681 aatgatcaag ttgcattaga tattcaaaaa cttatcgact tagatatcga tgtggtctta
    22741 ataagcaaca aacctaaaac agatgatttc cctgatcatc tcgtacattt tatcgattta
    22801 tctacaccaa gacctatagt ctacactgaa gattacgata agattgtcca accacatgcg
    22861 atggcattga attatgtata ctatgacatt tatacacaaa tgattgaaat gacaagagac
    22921 ttagaattat aaaagtcact attgttattt cataccttgt cactaacata atattaaaat
    22981 tatacgaaaa accggtactt tcaatgttta acaatgaaag taccggtttt attagcaaat
    23041 ttatttattt tgttgatatt ttaatgaagc ttcaataaat gatttaaaaa tcgggtgcgg
    23101 acgatttggt ctagataaga attctgggtg gaattgacaa gcaataaaga aatcatttgt
    23161 cggaatctct accatttcta ctaaacgtcc atctggactt gtaccagaaa tcaccatacc
    23221 atttgcttct aattgttctc tatagtcatt attaaattca taacgatgac gatgtctttc
    23281 ttcaatttcc gctttaccat aaacatcttg tgccaatgtg ccttctttaa ttgaacatgg
    23341 atataagcct aagcgtaatg taccacctaa atcttcgata tctttttgtt ctggtaataa
    23401 atctataatt gggtatggtg ttgctgggtc taattcagct gaatgtgcgc cttcaaggcc
    23461 taatacgttt cttgaaaatt caactgttgc aagttgcatt cctaaacaaa taccaaagaa
    23521 tggtacattg ttttctctag catacttaat tgcactaatt ttaccttcac ttgcacggaa
    23581 accaaatcca cctggtacta aaataccgtc gacatctgca aggtattcgg ctgcattttc
    23641 atctgttact tcacttgaat caatccatct aatgtcaata tctttggcaa aaggatatcc
    23701 agcatgtttc aatgattcaa caactgataa atatgcatct tgtaagctaa catatttacc
    23761 tactaaacca attgtaattt taccatctaa attattaacg atatctaaca actgtttcca
    23821 ttcatcaagc tgtgtttcat atttcgcgtt taattgtaaa cgtttaataa cgatatcatc
    23881 catattttgt tggcttaatt gtaatggaat ttcgtataaa gagtctgcat cacgacattc
    23941 aataacactt tctttattaa tgtcacagaa taatgcaatt ttatctttta aatcttgtgt
    24001 catttcatat tcagttctta caacgattaa gtctggttga atacctaagc ctcgtaattc
    24061 tttaacacta tgttgtgttg gcttcgtttt catttctcca gcagctttaa tataaggcag
    24121 taatgtacag tgaacataca taacattttc tctacctaaa tcgctacgaa tttgacgaat
    24181 cgcttcaata aacggtaatg actcaatatc acctgttgta ccgccaattt cagtgataac
    24241 aacgtctgca ttcgtacttt cccctgcaag taataaacgt tctttaattt catttgtaat
    24301 atgcggaata acttgaactg ttccgcctaa gtaatcacca cgacgttctt ttttcaatac
    24361 gtgtgaatac actttaccgg ctgtcacatt tgaaaacttg tttaaattaa tatcaataaa
    24421 tctttcgtaa tgtcctaagt ctaggtcagt ttctgcacca tcatccgtta cgaatacttc
    24481 accatgttga taaggactca ttgtacctgg gtcaacattt aagtatggat cgaatttttg
    24541 aattgttaca tttagacctc tatcttttaa taatctacct agagaagatg ctgtaatacc
    24601 cttccctaat gatgaaacta cgccacctgt tacaaaaata aattttgtca tgtcttgacc
    24661 tcctaattat taaagggaat gcttcctcat caattgtaaa aattacgtaa aacataacat
    24721 taaagcaagc tcattcaagt tgtttatttg tcgaattcga caaaattaaa ttctgtttac
    24781 ttaccgtttc catactgcta tgtcactatg tatatcattt acaactttga tttttgcgtt
    24841 atcatttctt gatttactca attaaataaa aaaacgctcc ctctcacaaa ttctgtaagg
    24901 ggagcgtata aattttatac acgtgtccta tttaaaggag cccgaataaa attttatcat
    24961 ctttcaacta aaagtcaaac aaaaaattaa tcgttgaagt cttcttcgtc ttcaaacact
    25021 tcttcgtctt cgtctagttc atcttcatct tcttcaatga ctatatccga atgattgatt
    25081 tcttcttcaa cctgctcatc ttctggatca tttagttctt cttgatcatc tgtttgagct
    25141 ggaatatcat cgtcgtcatc catttcatct tcgcccaata attttaagtt ttgatcttct
    25201 tcatcatctg catccagaat atcgaatttt tgaatagttg gtgcgatttt ctcttcaata
    25261 tcatctaccg aataccaatc acgtaatccc cataaatttt ctccaacatt taaaaaacga
    25321 ccatctgtgt ttaaatccgt gtaaaattgt acaacacgat tttcaatttc ttcgtactca
    25381 taatcaccta acgctctaaa ttcatcgatg atatcatata agttcattgt ttcgccttta
    25441 tcattcaata atgtataagc catatcaata aatgattttt catcaaccat ttgttttgta
    25501 taatcttgaa ttttcattgc ccaatacttc ctttcgaagg atataatcta aatttatacg
    25561 tctgaattca tttatatata gtaacaaaac taaattataa atgacaatat catatttcgc
    25621 aatcactgtt agtaatttaa atatttttcg aaaacgacat aatcatcagt ttgttcgatt
    25681 tcaacgaaat tactatttaa acataatggt ttaaaatcat tattctctcc aaacagaatc
    25741 atcgtaatca cattaaggtt gtcatagttt tttcttaagt aagttggcaa ttgtcgcaga
    25801 gcacttgaac caataccatt aaagcgatat gatttatcca cttcaatagc agcaatgttc
    25861 atgcgttcac caatctgctc caatgcgata aagccaacct tttggccatc aatcattaaa
    25921 ttaatgatat gtacattggt cacttctgaa cgactttctt cttcaagctt taatgatgaa
    25981 acatgttttg aatctaagtc caagtaatat agtcgttctt tgccaattgg tccttcttct
    26041 ttagtcgctg tatgcatgaa tccagcacgt tcgtataggt tccaagcatt gtcattttcc
    26101 gcatctacta ctagatatag atgattaaaa tcaggaaata caccttgaac atattgcggc
    26161 aatgacatca ttattttcgt gccatatcca aaaccttgat atttttcatt aatcgataat
    26221 gaacgaatat aaacgacatt ttcaggtgta tcataacctt catgctgata gtaacgatgt
    26281 aatacaaaaa agcccaccac ttcattttta tcgtttattg ctacgttagc aatcctatca
    26341 gcatcattaa ttgcatcatc aataacttct ttaggtaaag atgaatatat ttgttgtctt
    26401 tcattcaagt caaaatcatt aattgctgaa cgatattgtt catcaaatgc ttttaaagtg
    26461 ataccttcaa aacaaagttc tttagccata tatttaaccc ctatcttttt atatatgaag
    26521 catgaatccc catattattt tacctattat ttcactacga taccatta
//
LOCUS       DAXWFK010000017        25105 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.017, whole genome shotgun sequence.
ACCESSION   DAXWFK010000017 DAXWFK010000000
VERSION     DAXWFK010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 25105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25105
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.017"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            56..1351
                     /locus_tag="ACGEXS_000582"
     CDS             56..1351
                     /locus_tag="ACGEXS_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011629.1"
                     /translation="MSFLRKHAEIIFSYLIGIVSLFTGLIILINLPLIKQLNGGKKVD
                     THVHNVWEFLNAFFSEIIKVMSRFIGNFPIVSAIVIIIFGILVMLIGHTLLRTIKYDY
                     DISIFFLVIGIMYFIITLILMTQVYGFFAVIFIIPFTIHIGYIVYKDELNQENVKNHF
                     MWIIVSYGISYLITQIALYGRIDANEIESIDILSVNAFFIIMWLLGQMAIWNFLFLRR
                     ALPLTKQELGEEEPELSRTSKGNVTNQTKIHLKQLQDKTTEYARKTRRSVDLDKIRAK
                     RDKFKKKVNDIIDIQEDDIPDWMRKPKWVKPMYVELFCGVVIFLFTFLEFNNRNALFV
                     SGDWKLSQTQYVIEWVTLLILLFIIIAYIATTLTFHLKGKFYYLQLFMGSILFFKLLT
                     EFINIMIHGLLLSVFITPTLLLMLLAIIISYSLQLRERP"
     gene            1776..2948
                     /locus_tag="ACGEXS_000583"
     CDS             1776..2948
                     /locus_tag="ACGEXS_000583"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFF8011630.1"
                     /translation="MKIATLNKGKETKYFNGYPLIEEEDIYSQDHLKEGDIFQIVTDK
                     SQYVATAYVGRQHKGLGWVLTYDKAQQINTAFFVKLFNTALAERDYYFNIDGTNAFRL
                     FNAEGDGVGGLTIDNYDGHLLIQWYSKGIYKFKYAILEAVRKVFDYKSIYEKVRFKDS
                     EYSGGFVEGDAPEFPIVIEENFTFYNVDLEDGLMTGIFLDQKEVRKKLRDQYAKERHV
                     LNLFSYTGAFSAIAASEASSTTSVDLANRSRSLTEENFGLNAIDPKSQYIYVMDTFDF
                     YKYAARHGHSYDTIVIDPPSFARNKKRTFSVQKDYDKLINGALNILSSEGTLLLCTNA
                     SVYPLKQFKNTIKKTLEESGVDYELTEVMGLPKDFKTHPHYKPSKYLKAVFVNIRH"
     gene            3002..3544
                     /locus_tag="ACGEXS_000584"
     CDS             3002..3544
                     /locus_tag="ACGEXS_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HFF8011631.1"
                     /translation="MGFKNNLTSNLTNKIGNSVFKIENVDGKGAMPTTIQELRERRQR
                     AEAIVKRKSLMSSTMSVVPIPGLDFGVDLKLMKDIIEDVNKIYGLDHKQVNSLGDDVK
                     ERIMSAAAIQGSQFIGKRISNAFLKIVIRDVAKRTAAKQTKWFPVVGQAVSASISYYF
                     MNKIGKDHIQKCENVIKNVM"
     gene            3698..3964
                     /locus_tag="ACGEXS_000585"
     CDS             3698..3964
                     /locus_tag="ACGEXS_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFF8011632.1"
                     /translation="MEQNSYVIIDETGIHARPATMLVQTASKFDSDIQLEYNGKKVNL
                     KSIMGVMSLGVGKDAEITIYADGSDESDAIQAISDVLSKEGLTK"
     gene            3967..5685
                     /gene="ptsP"
                     /locus_tag="ACGEXS_000586"
     CDS             3967..5685
                     /gene="ptsP"
                     /locus_tag="ACGEXS_000586"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFF8011633.1"
                     /translation="MSKLIKGIAASDGVAIAKAYLLVEPDLTFDKNEKVTDVEGEVAK
                     FNSAIEASKVELTKIRNNAEVQLGADKAAIFDAHLLVLDDPELIQPIQDKIKNENANA
                     ATALTDVTTQFVTIFESMDNEYMKERAADIRDVSKRVLSHILGVELPNPSMIDESVVI
                     VGNDLTPSDTAQLNKEFVQGFATNIGGRTSHSAIMSRSLEIPAIVGTKSITQEVKQGD
                     MIIVDGLNGDVIVNPTEDELIAYQDKRERYFADKKELQKLRDADTVTVDGVHAELAAN
                     IGTPNDLPGVIENGAQGIGLYRTEFLYMGRDQMPTEEEQFEAYKEVLEAMGGKRVVVR
                     TLDIGGDKELSYLNLPEEMNPFLGYRAIRLCLAQQDIFRPQLRALLRASVYGKLNIMF
                     PMVATINEFREAKAILLEEKENLKNEGHDISDDIELGIMVEIPATAALADVFAKEVDF
                     FSIGTNDLIQYTLAADRMSERVSYLYQPYNPSILRLVKQVIEASHKEGKWTGMCGEMA
                     GDETAIPLLLGLGLDEFSMSATSILKARRQINGLSKNEMTELANRAVDCATQEEVIEL
                     VNNYVK"
     gene            complement(5922..6155)
                     /gene="nrdH"
                     /locus_tag="ACGEXS_000587"
     CDS             complement(5922..6155)
                     /gene="nrdH"
                     /locus_tag="ACGEXS_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFF8011634.1"
                     /translation="MSEIIVYTQNDCPPCTFVKNYLNEHHIDFEERNINNQQYRNEMI
                     DFDAFSTPFILLNGNPMYHVDLDEINKVLNIQD"
     gene            6353..7714
                     /locus_tag="ACGEXS_000588"
     CDS             6353..7714
                     /locus_tag="ACGEXS_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFF8011635.1"
                     /translation="MDTVEISRFLTAMTLAVHIIFATIGVGMPLMFAIAEFLGIRKND
                     LQYIAMAKRWAKAYTITVAVGVVTGTIIGLQLSLIWPTFMEMGGHVIALPLFMETFAF
                     FFEAIFLSIYLYTWDRFKNKWTHFLISIPVIIGGSFSAFFITSVNSFMNTPAGFELKN
                     GKMVNVQPIEAMFNPSFIVRSFHVITTAGMTMAFVIASIAAFKLLRNRQPKDTVYHKK
                     ALKMSMIVGFFSTLLSMLAGDLSAKFLHKFQPEKLAAYEWHFDTSSHAKLLLFGVLDE
                     KTQQVKGAIELPGLLSFLADNSVKTKVQGLNDFPKSLHPPMIVHYFFDLMVTMGILCF
                     VISGVYVLTLMFKKLRKFSTHKWMLYGILLTGPASMLAIEFGWFLTEMGRQPWIVRGY
                     MRVAEAATQAGGITFVTILFGILYIILMYTCAYVLIRMFKNKPAYEDVNRLAKKQGGE
                     IEK"
     gene            7711..8730
                     /locus_tag="ACGEXS_000589"
     CDS             7711..8730
                     /locus_tag="ACGEXS_000589"
                     /EC_number="7.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006119 - oxidative phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFF8011636.1"
                     /translation="MIYAFIGITVLWLFLFCYIIIASIDFGAGFFALHSKLTGDEKKI
                     NHLISRYLNPVWEVTNVFFVFFFVGFVGFFPESIKYLGTVLLIPGSIALIMISLRNSF
                     YAFENYGQDTKLAWMIMYGVSGLLIPASLSTALTITEGGYINVRNNVIDLDWVQLLLS
                     PFAWSVVFLAIISVLYISSGFLTYYAKKANDEPAYNLTRQWHIFLGPPMIIICLFVFL
                     SLRIQNSEHFYSAVFDYWWMFGISFLFFALASLLTFFKKKHGLAFVFVILQMMFAFFG
                     YGISKLPYLLYPFVKITDAYVNPEMGWTLVIVFILGLLLLLPSLILLLRLFVFDKEYV
                     EGKKS"
     gene            8863..9525
                     /locus_tag="ACGEXS_000590"
     CDS             8863..9525
                     /locus_tag="ACGEXS_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFF8011637.1"
                     /translation="MGKEYVVIGLGRFGGSIVRELNALDMDVMAIDHDENRVNEYSDI
                     ATHAVVADTTDEAVMKSLGIRNFDHVIVAIGENIQSSTLTTLILKELGVKKVTAKAQN
                     DYHAKILNKIGADTVVHPERDMGRRIAHNVASASVLDYLELADEHSIVELKATEKMAG
                     QSIIDLDIRAQYGINIIAIKRGKEFIISPNPNINLEIGDILIMIGHDNDLNRFEKNIA
                     TR"
     gene            complement(9874..11571)
                     /locus_tag="ACGEXS_000591"
     CDS             complement(9874..11571)
                     /locus_tag="ACGEXS_000591"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFF8011638.1"
                     /translation="MKQLHPNEVGVYALGGLGEIGKNTYAVEYKDEIVIIDAGIKFPD
                     DNLLGIDYVIPDYTYLVQNQDKIVGLFITHGHEDHIGGVPFLLKQLNIPIYGGPLALG
                     LIRNKLEEHHLLRTAKLNEINEDSVIKSKHFTISFYLTTHSIPETYGVIVDTPEGKVV
                     HTGDFKFDFTPVGKPANIAKMAQLGEEGVLCLLSDSTNSLVPDFTLSEREVGQNVDKI
                     FRNCKGRIIFATFASNIYRVQQAVEAAIKNNRKIVTFGRSMENNIKIGMELGYIKAPP
                     ETFIEPNKINTVPKHELLILCTGSQGEPMAALSRIANGTHKQIKIIPEDTVVFSSSPI
                     PGNTKSINRTINSLYKAGADVIHSKISNIHTSGHGSQGDQQLMLRLIKPKYFLPIHGE
                     YRMLKAHGETGVECGVEEDNVFIFDIGDVLALTHDSARKAGRIPSGNVLVDGSGIGDI
                     GNVVIRDRKLLSEEGLVIVVVSIDFNTNKLLSGPDIISRGFVYMRESGQLIYDAQRKI
                     KTDVISKLNQNKDIQWHQIKSSIIETLQPYLFEKTARKPMILPVIMKVNEQKESNNK"
     gene            complement(11571..11789)
                     /locus_tag="ACGEXS_000592"
     CDS             complement(11571..11789)
                     /locus_tag="ACGEXS_000592"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase subunit epsilon"
                     /protein_id="HFF8011639.1"
                     /translation="MAVFKVFYQHNRDEVIVRENTQSLYVEAQTEEQVRRYLKDRNFN
                     IEFITKLEGAHLDYEKENSEHFNVEIAK"
     gene            complement(11818..11940)
                     /locus_tag="ACGEXS_000593"
     CDS             complement(11818..11940)
                     /locus_tag="ACGEXS_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011640.1"
                     /translation="MNDMMNLVINQIVIKLHYRDDWNEFIDIYSNTILLGLTIN"
     gene            complement(12270..12821)
                     /gene="def"
                     /locus_tag="ACGEXS_000594"
     CDS             complement(12270..12821)
                     /gene="def"
                     /locus_tag="ACGEXS_000594"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFF8011641.1"
                     /translation="MLTMKDIIRDGHPTLRQKAAELELPLTKEEKETLIAMREFLVNS
                     QDEEIAKRYGLRSGVGLAAPQINISKRMIAVLIPDDGSGKSYDYMLVNPKIVSHSVQE
                     AYLPTGEGCLSVDDNVAGLVHRHNRITIKAKDIEGNDIQLRLKGYPAIVFQHEIDHLN
                     GVMFYDHIDKDHPLQPHTDAVEV"
     gene            13186..13812
                     /locus_tag="ACGEXS_000595"
     CDS             13186..13812
                     /locus_tag="ACGEXS_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkyA family protein"
                     /protein_id="HFF8011642.1"
                     /translation="MKFGKTIAVVLASSVLLAGCTTDKKEIKAYLKQVDKIKDDEEPI
                     KTVGKKIAELDEKKKKLTEDVNSKDTAVRGKAVKDLIKNADDRLKEFEKEEDAIKKSE
                     QDFKKAKSHVDNIDNDVKRKEVKQLDDVLKEKYKLHSDYAKAYKKAVNSEKTLFKYLN
                     QNDATQQGVNEKSKAIEQNYKKLKEVSDKYTKVLNKVGKEKQDVDQFK"
     gene            13983..15095
                     /gene="pdhA"
                     /locus_tag="ACGEXS_000596"
     CDS             13983..15095
                     /gene="pdhA"
                     /locus_tag="ACGEXS_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002467471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFF8011643.1"
                     /translation="MAPKLQAQFDAVKVLNDTQSKFEMVQILDENGNVVNEDLVPDLT
                     DEQLVELMERMVWTRILDQRSISLNRQGRLGFYAPTAGQEASQLASQYALEKEDYILP
                     GYRDVPQIIWHGLPLTEAFLFSRGHFKGNQFPEGVNALSPQIIIGAQYIQAAGVAFAL
                     KKRGKNAVAITYTGDGGSSQGDFYEGINFAAAYKAPAIFVIQNNNYAISTPRSKQTAA
                     ETLAQKAIAVGIPGIQVDGMDALAVYQATKEARDRAVAGEGPTLIETMTYRYGPHTMA
                     GDDPTRYRTSDEDAEWEKKDPLVRFRKFLENKGLWNEDKENEVIERAKADIKAAIKEA
                     DNTEKQTVTSLMEIMYEDMPQNLAEQYEIYKEKESK"
     gene            15099..16076
                     /locus_tag="ACGEXS_000597"
     CDS             15099..16076
                     /locus_tag="ACGEXS_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017637695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HFF8011644.1"
                     /translation="MAQMTMVQAINDALKTELKNDQDVLIFGEDVGVNGGVFRVTEGL
                     QKEFGEDRVFDTPLAESGIGGLAMGLAVEGFRPVMEVQFLGFVFEVFDAIAGQIARTR
                     FRSGGTKTAPVTIRSPFGGGVHTPELHADNLEGILAQSPGLKVVIPSGPYDAKGLLIS
                     SIRSNDPVVYLEHMKLYRSFREEVPEEEYTIDIGKANVKKEGNDISIITYGAMVQESM
                     KAAEELEKDGYSVEVIDLRTVQPIDVDTIVASVEKTGRAVVVQEAQRQAGVGAAVVAE
                     LSERAILSLEAPIGRVAAADTIYPFTQAENVWLPNKNDIIEKAKETLEF"
     gene            16167..17459
                     /locus_tag="ACGEXS_000598"
     CDS             16167..17459
                     /locus_tag="ACGEXS_000598"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HFF8011645.1"
                     /translation="MAFEFRLPDIGEGIHEGEIVKWFVKAGDTIEEDDVLAEVQNDKS
                     VVEIPSPVSGTVEEVMVEEGTVAVVGDVIVKIDAPDAEDMQFKGHDDDSSSKEEPAKE
                     EAPAEQAPVATQTEEVDENRTVKAMPSVRKYAREKGVNIKAVSGSGKNGRITKEDVDA
                     YLNGGAPTASNESAASATSEEVAETPAAPAAVTLEGDFPETTEKIPAMRRAIAKAMVN
                     SKHTAPHVTLMDEIDVQALWDHRKKFKEIAAEQGTKLTFLPYVVKALVSALKKYPALN
                     TSFNEEVGEIVHKHYWNIGIAADTDRGLLVPVVKHADRKSIFQISDEINELAVKARDG
                     KLTADEMKGATCTISNIGSAGGQWFTPVINHPEVAILGIGRIAQKPIVKDGEIVAAPV
                     LALSLSFDHRQIDGATGQNAMNHIKRLLNNPELLLMEG"
     gene            17463..18869
                     /gene="lpdA"
                     /locus_tag="ACGEXS_000599"
     CDS             17463..18869
                     /gene="lpdA"
                     /locus_tag="ACGEXS_000599"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFF8011646.1"
                     /translation="MVVGDFPIETDTIVIGAGPGGYVAAIRAAQLGQKVTIVEKGNLG
                     GVCLNVGCIPSKALLHASHRFVEAQHSENLGVIAESVSLNFQKVQEFKSSVVNKLTGG
                     VEGLLKGNKVNIVKGEAYFVDNNSLRVMDEKSAQTYNFKNAIIATGSRPIEIPNFKFG
                     KRVIDSTGALNLQEVPGKLVVVGGGYIGSELGTAFANFGSEVTILEGAKDILGGFEKQ
                     MTQPVKKGMKEKGVEIVTEAMAKSAEETDNGVKVTYEAKGEEKTIEADYVLVTVGRRP
                     NTDELGLEELGVKFADRGLLEVDKQSRTSISNIYAIGDIVPGLPLAHKASYEAKVAAE
                     AIDGQAAEVDYIGMPAVCFTEPELATVGYSEAQAKEEGLAIKASKFPYAANGRALSLD
                     DTNGFVKLITLKEDDTLIGAQVVGTGASDIISELGLAIEAGMNAEDIALTIHAHPTLG
                     EMTMEAAEKAIGYPIHTM"
     gene            19037..19312
                     /locus_tag="ACGEXS_000600"
     CDS             19037..19312
                     /locus_tag="ACGEXS_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFF8011647.1"
                     /translation="MEYEYPIDLDWSNEEMISVINFFNHVEKYYESGVTAGDFMGAYK
                     RFKEIVPAKAEEKQIFNTFEKSSGYNSYKAVQDVKTHSEEQRVTAKK"
     gene            19456..19995
                     /locus_tag="ACGEXS_000601"
     CDS             19456..19995
                     /locus_tag="ACGEXS_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFF8011648.1"
                     /translation="MNIGNKIKNLRRIKNLTQEELAERTDLSKGYISQIESEHASPSM
                     ETFLNIIEVLGTTPSEFFKDSENEKVLYKKEEQVIYDEYDEGYILNWLVSKSNEYDME
                     PLILTLKPGASYKNFNPSESDTFIYCMSGQITLNLGKEIYQAQEEDVLYFKARDNHRL
                     SNESNNETRILIVATASYL"
     gene            20008..21102
                     /locus_tag="ACGEXS_000602"
     CDS             20008..21102
                     /locus_tag="ACGEXS_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8011649.1"
                     /translation="MEPLLSLKSVSKSYDDLNILDDIDIDIESGYFYTLLGPSGCGKT
                     TILKLIAGFEYPDSGEVIYQNKPIGNLPPNKRKVNTVFQDYALFPHLNVYDNIAFGLK
                     LKKLSKTEIDQKVTEALKLVKLSGYEKRNINEMSGGQKQRVAIARAIVNEPEILLLDE
                     SLSALDLKLRTEMQYELRELQSRLGITFIFVTHDQEEALALSDFLFVLKDGKIQQFGT
                     PTDIYDEPVNRFVADFIGESNIVEGRMVRDYVVNIYRQDFECVDMGIPENKKVEVVIR
                     PEDISLIKAEEGLFKATVDSMLFRGVHYEICCIDNKGYEWVIQTTKKAEVGSEVGLYF
                     DPEAIHIMVPGETEEEFDKRIESYEEVDNA"
     gene            21095..21892
                     /locus_tag="ACGEXS_000603"
     CDS             21095..21892
                     /locus_tag="ACGEXS_000603"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8011650.1"
                     /translation="MRNTNKFLLIPYLLWMVIFIIVPVVLLIYFSFLDINGHFSFTNY
                     QQIFTTKYLKMFAYSILYAASITIITLTISYPAAYYITRSKFQNILLMIMIIPTWINL
                     LLKTYAFIGLLSHDGVINQFFHLFNLPSFNLLFTTGAFLVVASYIYIPFMILPIFNSM
                     KAIPNNLLQASSDLGASPFYTFRKVIMPLTKEGVMTGIQVTFIPSLSLFMITRLIAGN
                     KVINIGTAIEEQFLTIQNYGMGSTIAIFLIVFMAFILIITKSSNGRG"
     gene            21898..22707
                     /locus_tag="ACGEXS_000604"
     CDS             21898..22707
                     /locus_tag="ACGEXS_000604"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8011651.1"
                     /translation="MKWYGKLYIGILLAILYIPIFFLMFYSFNSAGNMIHFEHFTLEH
                     YQSLFQNDHLMSVIFNTIAVALLAASISTVIGTFGAIAIYYLRNKKFKVTLLTLNNVL
                     MVSSDVVIGASFLIMFTTIGHFTGLGLGFWTVLISHIAFCIPIVVIIVLPQLYEMNNN
                     MLNAARDLGATEPQLLSNIIIPNILPSIIGGFFMALTYSLDDFTVSFFVTGNGFSVLS
                     VEVYAMARKGISMEINAISTLLFAVIVLGILGYYLIQYVINKKKLIKRGVK"
     gene            22707..23780
                     /locus_tag="ACGEXS_000605"
     CDS             22707..23780
                     /locus_tag="ACGEXS_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HFF8011652.1"
                     /translation="MKRFLQLIIGALVVGMLCLTLSHWFKSKEQVHTNQKIYVYNWGE
                     YIDPELIKKFEKETGIQVVYETFDSNEAMEAKIRNGGTHYDVAFPSEYTVQKLKRDHL
                     LLPIDHNKVPNIKNLDSDYMNMSFDRGNKYSLPYFFGTVGILYNKEKYPNESFDSWKS
                     LYNPKFKNQILLVDGAREIIGMSLNKLGYNLNDRNSHHLKEAERDLTKLAPQVRGVVG
                     DEITMMLQQNEGNIAVVWSGVAAPLVQEGDKYNYVIPKEGSNLWFDNMVIPKTAQNKE
                     GAYKFMNFLLDAKNNKQNTEFVGYATPNKAARQLLPKEIKDDHRFYPTKKEQERLEVY
                     KDLGPEVLSEYNENFLNFKMSLK"
     gene            23854..24873
                     /locus_tag="ACGEXS_000606"
     CDS             23854..24873
                     /locus_tag="ACGEXS_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFF8011653.1"
                     /translation="MTGEQFTQIKRPVSRLTEKVLGWLCWVMLLVLTVITMFIALVSF
                     SNNTSIANLENTLNNNAFIQQLLAGNGYNTTQFVIWLQNGIWAIIVYFIVCLLISFLA
                     LISMNIRILSGFLFLISAIVTIPLVLLIVTLIIPILFFIIAMMLFIRKDKVEMVAPQY
                     YEEYNGPIYDYREPVYERLQPKDDYYDVPKYEKELDKSNTVYDQEQERDKYDQFPKRA
                     VESEYNHDERTEEEPSVLSRQAKYKQKSTEELGIEDDGYYAEPEVDPKELKAQQKREK
                     AEIKAKKKEKRKAYNQRMKERRKNQPSAVSQRRMNFEERRQIYNNDISEERNSSEVKD
                     KKEQE"
BASE COUNT         8798 a         3531 c         4569 g         8207 t
ORIGIN      
        1 tttatctaaa gacaacaaca ttagattaat atataatgat tttgaggtga acataatgtc
       61 ttttcttagg aaacacgccg aaattatttt tagctattta atcggtatcg tttcactctt
      121 cactggtctc attattttaa ttaacttgcc attaattaaa caattaaatg gtggtaaaaa
      181 agttgataca catgttcata atgtgtggga atttctgaat gcatttttca gtgaaattat
      241 taaagtaatg agtcgattta taggtaattt ccctatagtt agtgcaattg tgataattat
      301 attcggtatt ttagttatgt tgattggtca tacattactt agaactatta agtatgacta
      361 tgatatttct atctttttct tagttatcgg tatcatgtac tttattatta ctcttatatt
      421 aatgactcaa gtttatggat tctttgcagt gattttcatt attccattta caattcatat
      481 aggatatatc gtctataaag atgaattgaa tcaggaaaat gtaaaaaatc atttcatgtg
      541 gataattgtg agttatggta taagttactt aattacacaa attgcattgt atggcagaat
      601 tgatgctaat gaaatagagt caattgatat cttaagtgtc aatgctttct ttataattat
      661 gtggttactt ggtcaaatgg ctatttggaa tttcttgttc ttgcgccgag ctttaccttt
      721 aacaaagcaa gaattaggtg aagaggagcc agaattatca agaacaagta aagggaatgt
      781 cacgaatcaa actaaaattc acttgaaaca actccaagat aagactacag aatatgcacg
      841 taagacaaga agaagtgtcg atttagataa aattagagct aaaagagata aattcaaaaa
      901 gaaagttaat gatattatcg atattcaaga agacgatatt cctgattgga tgagaaaacc
      961 gaaatgggtt aaaccaatgt atgtcgaact attttgtggt gtcgtcatct ttttattcac
     1021 atttttagaa tttaataatc gtaatgcatt atttgtatct ggtgattgga aattatcaca
     1081 gacacaatat gttattgaat gggttacatt attaattctg ttattcatta ttatcgcata
     1141 tatcgctaca acgttaactt tccacttgaa aggtaagttt tattatttac aattatttat
     1201 ggggagcatt ttattcttta aattgttaac ggaatttata aatataatga ttcatggact
     1261 attactttca gtgttcatta cgccaacatt actattaatg ttattggcaa tcatcatttc
     1321 ttattcgtta caattacgag agcgaccata attaaaagca ttataaaagt actatctatt
     1381 aaacattttg atgtgtacgc tataagttag atatatctct aacttactta gatacaggtc
     1441 aatgaagttt atggatagta ctttttttgt aactagattt gattgattca ggtgatgtga
     1501 attaagtatt gaaaattgta tacaaagttt tagtgcaaat aaaatagttg aaaagttatc
     1561 catttgtaaa atcaagaaaa ctagtaaata gttgaagcga cttatggaat ttgcgaaacg
     1621 atatatagta tttcctttgt agaaatttaa catatatcat tcgaattact aatttgttaa
     1681 aatcaacagt aagattagaa gtagatgata ttgaaatttg gcaaacaatt taatctatat
     1741 aaaactacaa cgaaacacag aaaggaagtt gtcagatgaa aatagcaact ctgaacaaag
     1801 gcaaagaaac aaaatatttt aatggatatc ctttaattga agaagaggat atctattcac
     1861 aagatcattt aaaagaagga gatatttttc aaattgtgac tgataaatca caatatgtcg
     1921 caacggctta tgttggtcgc caacataaag gattaggttg ggttctaaca tacgataaag
     1981 ctcaacaaat caacacagct ttctttgtaa aattgtttaa tactgcatta gcagaacgtg
     2041 attattattt taatatagat ggaacaaatg cttttagatt atttaatgct gaaggtgatg
     2101 gtgttggggg attaacaatc gacaattacg atggtcattt gttgattcaa tggtactcaa
     2161 aaggtattta taaatttaaa tatgccattc ttgaagcggt tagaaaagta tttgattata
     2221 aatctattta cgaaaaagta agatttaaag acagcgaata tagtggtggt tttgttgaag
     2281 gagatgcacc tgagtttcca attgttatcg aagaaaactt cacattttat aatgtagacc
     2341 ttgaagatgg tttgatgaca ggtatctttt tagatcaaaa agaagtgcgc aagaaattaa
     2401 gggatcaata tgccaaagaa cgccatgttt taaacttatt tagttataca ggtgcttttt
     2461 ctgcaatagc agcaagtgag gcatcttcaa caacaagtgt agatttggct aatcgttctc
     2521 gtagtttaac tgaagaaaat tttggattaa atgctattga tcctaaatcc caatatattt
     2581 atgtcatgga cacttttgat ttctataaat atgctgcacg acatggacat agttatgaca
     2641 cgatcgtgat tgatccacct agctttgcgc gtaacaaaaa acgtacattt tcagtgcaaa
     2701 aagattatga caaattaatt aatggcgcct taaatatctt atcatctgaa ggaacattat
     2761 tgttatgtac aaacgcaagt gtatatccat taaagcaatt taaaaatact attaaaaaga
     2821 cgcttgaaga gagtggcgtt gattatgaat taactgaagt tatgggatta ccaaaagatt
     2881 ttaaaacgca tccacattat aagccatcta aatatttaaa agctgttttt gtaaatatta
     2941 gacattaatt tgataaaaag gggtatagta acttattgag aaaaagaagg gtgataatat
     3001 tatgggattc aaaaacaatt taacatcaaa tttaacaaat aaaatcggta attcagtctt
     3061 taaaatagaa aatgttgacg gaaaaggtgc aatgccaacg acgattcaag aattgagaga
     3121 aagacgacaa cgtgctgaag caattgtaaa gagaaagtct ttaatgtcat caacaatgag
     3181 cgttgttcca attccgggtt tagattttgg tgttgattta aaattaatga aagatattat
     3241 cgaagatgtt aataaaattt atggtttaga tcataagcaa gttaatagcc ttggggatga
     3301 tgtgaaagaa agaattatgt ctgcagcagc aattcaaggt agtcaattta ttggtaaaag
     3361 aatttcaaat gcatttttaa aaattgtaat tagagatgta gctaaacgta ctgctgcaaa
     3421 acaaacaaaa tggtttcctg ttgtaggaca agctgtgtct gcatctatta gttactattt
     3481 tatgaataaa attggaaaag atcacattca aaaatgcgaa aatgttatta aaaatgtcat
     3541 gtaggtgcta taatagtttt gcaatttgca aattttactg aaaccggttt taaacgaatt
     3601 gaatttaaag catggttttg gtaaagttaa tgtataaaac taagttagta ttgtaataat
     3661 attgaagatt ctaactatac gaaggagaaa tgtaattatg gaacaaaatt catatgtaat
     3721 catcgacgag actggtattc acgctagacc agcaacaatg ttagtacaaa cagcttcaaa
     3781 attcgattct gatattcaat tagaatataa cggtaagaaa gtaaacttaa aatcaatcat
     3841 gggtgttatg agccttggtg ttggtaaaga tgctgaaatt acaatttatg ctgacggtag
     3901 tgatgaatct gacgccattc aagcaatcag tgacgtctta tcaaaagaag gattgactaa
     3961 ataatcatgt ctaaattaat taaaggtatt gccgcatctg atggtgtcgc aattgctaaa
     4021 gcttatttat tagttgagcc agacttaaca ttcgacaaaa atgaaaaagt cactgatgtt
     4081 gaaggagaag ttgcaaagtt caatagcgct atcgaagctt ctaaagttga gttaactaaa
     4141 attagaaata atgcagaggt tcaactaggt gctgataaag ctgctatctt tgatgcacat
     4201 ttattagttt tagatgaccc tgaattaatt caaccaatcc aagataagat taaaaatgaa
     4261 aacgctaatg ctgctacagc attaacggat gtaacaacac aatttgttac aatttttgaa
     4321 tctatggata acgaatacat gaaagaacgt gcggctgata ttcgcgacgt ttctaaacgt
     4381 gtgttatcac atattttagg tgtagaatta ccgaatccga gtatgattga tgaaagcgtt
     4441 gttattgtag ggaatgactt aacgccatct gatactgctc aattaaataa agaattcgta
     4501 caaggttttg ctacaaacat tggcggaaga acaagtcact ctgcaattat gagtcgttct
     4561 ttagaaattc cagcaattgt tggtacaaaa tcaattactc aagaagttaa acaaggcgac
     4621 atgattatcg tagatggatt aaatggtgat gtaatcgtta atccaactga agatgagtta
     4681 atcgcttatc aagataaacg tgagcgttat tttgctgaca agaaagaatt acaaaaacta
     4741 cgtgatgctg atactgttac agttgatggt gttcacgcag agcttgctgc aaatattggt
     4801 acacctaatg atttgccagg tgttattgaa aatggtgcac aaggtatcgg cttatataga
     4861 actgagtttt tatatatggg tcgtgaccaa atgcctacag aagaagaaca atttgaagct
     4921 tataaagaag tattagaagc aatgggcggt aaacgtgttg ttgtacgtac tttagatata
     4981 ggtggagata aagaattatc atacttaaac ttgcctgaag aaatgaatcc attcttaggt
     5041 taccgtgcga ttcgtttatg ccttgcgcaa caagatattt tcagaccaca gctacgtgca
     5101 ttattacgtg catcagttta tggtaagtta aatatcatgt tcccaatggt tgcaacaatt
     5161 aacgaattta gagaagctaa agctatatta ttagaagaaa aagaaaacct taaaaatgaa
     5221 ggtcatgaca tttcggatga tatagaatta ggaatcatgg tagagatacc tgcaacagca
     5281 gcattagctg atgtctttgc taaagaagta gatttcttca gtatcggtac aaatgattta
     5341 attcaataca cattagctgc tgaccgtatg tcagagcgtg tatcatatct ataccaacca
     5401 tataaccctt caatcttacg tttagttaaa caagttattg aagcgtcaca taaagaaggt
     5461 aaatggacag gtatgtgtgg tgaaatggct ggagatgaaa cagctattcc attattgctt
     5521 ggtttaggtt tagatgagtt ctctatgagt gcaacgtcta ttctgaaagc aagaagacaa
     5581 attaatggtt taagtaaaaa tgaaatgact gaacttgcta accgtgcagt cgactgtgca
     5641 acgcaagaag aagttattga attagttaac aactacgtaa aataattaat tctttcgaaa
     5701 gacagaatta taaataaacc cgaagtattc attgtaagta attatgaata cttcgggttc
     5761 ttttttatga tttatgttaa tttcaatgga atgttgttgc taactaatta aacaactatg
     5821 tgtcattaac aaaatgtagt tctttaatta atgttagtta atttaaaaat cgatatttaa
     5881 tttatgatag ctataaatga tgaagttgat ttagatgaaa attaatcttg gatatttaat
     5941 actttgttga tttcatcaag atcaacatgg tacattggat tgccattcaa caaaataaac
     6001 ggagttgaaa aagcatcaaa atctatcatt tcgtttcgat attgttgatt gttgatattt
     6061 ctctcttcaa aatcaatgtg atgctcattt agataatttt ttacaaatgt acaaggtgga
     6121 caatcattct gcgtataaac gattatttct gacatgtatc atgctccttc gtgctttatt
     6181 ctaatgtaat atacacaatt atacattgaa attcaaattt gtgaacacat tgtgaactga
     6241 cataaattat acacaatttc atagcgcata atgttttaaa aatacacatt atagcaatta
     6301 taattaagtt cattgtgttg tcatcaaaaa aagaaaaagg tgatgttttt aaatggatac
     6361 agttgaaatc agtcggtttt tgacagctat gactttagca gttcatatca tttttgcaac
     6421 gattggtgtt ggtatgcctt taatgttcgc aattgcagaa tttttaggta ttcgcaaaaa
     6481 tgatcttcaa tatatagcta tggccaaaag atgggctaaa gcttatacaa ttactgtagc
     6541 agtgggagtt gttacaggta caattatagg acttcaatta tcattgattt ggcctacatt
     6601 tatggaaatg ggtggacacg ttattgcact tccattattt atggaaacat ttgcgttctt
     6661 ctttgaagct attttcttaa gtatatattt atatacttgg gatcgtttta aaaataaatg
     6721 gacacatttc ttaattagta taccagtaat tattggtggc tctttctcag cattcttcat
     6781 tacttcagtg aattcattta tgaatacgcc tgcaggtttt gagttgaaga atggaaagat
     6841 ggtcaatgtt caacctatag aagcgatgtt taacccatcg tttatagttc gatcatttca
     6901 cgtaattact acagccggta tgacgatggc atttgttatt gcatcaatag cagcttttaa
     6961 attattgcgt aatcgtcaac ctaaagatac tgtctaccat aagaaagctt tgaaaatgtc
     7021 tatgatagtt ggattctttt caacattact ttctatgttg gcaggggatt tatctgcaaa
     7081 atttttgcat aaattccaac ctgaaaaatt agcagcttat gaatggcatt tcgatacatc
     7141 ttcccatgcc aaattattat tatttggtgt gttagatgaa aagactcagc aagttaaagg
     7201 tgcgattgaa ttacctggac tattgagttt cttagcagat aatagtgtca aaactaaagt
     7261 acaagggtta aatgattttc caaaaagttt acatccgcct atgatcgtcc attatttctt
     7321 tgatttaatg gtaacgatgg gaattttatg ttttgtcatt tcaggtgttt atgtcttaac
     7381 tttaatgttt aaaaagctta gaaagttttc tactcataaa tggatgcttt atggaatatt
     7441 attaacaggc ccagcttcaa tgctagctat agaatttgga tggttcttaa cagagatggg
     7501 tagacagcct tggattgttc gtggttatat gcgcgtggca gaagcagcaa cacaagcagg
     7561 cggaataacc ttcgttacaa ttttatttgg catattgtac atcattttaa tgtatacatg
     7621 tgcatacgta ttaattcgta tgtttaaaaa taaaccggcg tatgaagatg taaatcgttt
     7681 agccaagaag caaggaggag aaatagaaaa atgatttatg catttatagg tataacagtg
     7741 ttatggttgt ttttattttg ctatatcatt attgcttcta tagattttgg ggcaggtttc
     7801 ttcgcattgc attcaaagtt aactggtgat gaaaagaaaa ttaatcactt aatttcacgt
     7861 tatttaaacc cagtttggga agttacgaat gtattctttg ttttcttctt cgtaggattc
     7921 gtaggtttct ttccagagtc aatcaaatat ttaggcacgg tattgttaat accaggttca
     7981 atagcactga ttatgatatc gttgagaaac agtttttatg catttgaaaa ttatggtcaa
     8041 gatacaaaat tagcatggat gatcatgtat ggggtaagtg gattattaat tccagcttca
     8101 ttatctactg ctttaactat tacagaaggt ggctatatta atgttcgaaa caatgttatc
     8161 gatctagatt gggtgcagtt attattaagc ccatttgctt ggtctgtagt attcttggca
     8221 attatttcag ttttatatat ttcatcagga tttttgacat attatgctaa aaaagcaaat
     8281 gacgaaccag catataattt aacaagacaa tggcacatat ttttagggcc gccgatgatt
     8341 atcatttgtt tattcgtatt tctatcatta cgcattcaaa attctgaaca tttttattca
     8401 gctgtttttg actattggtg gatgtttggt ataagtttct tattctttgc attagcttca
     8461 ttgttaacat tctttaagaa aaaacatggt ttggcttttg tatttgttat tttacaaatg
     8521 atgttcgcgt tctttggcta tggaattagt aaattgccat accttttata tccgtttgta
     8581 aaaattacag atgcatatgt taatccagaa atgggctgga cattagtgat tgtctttatt
     8641 ttaggtttac ttttattact tccatcgtta atattattat taagattatt tgttttcgac
     8701 aaagaatatg ttgaaggaaa gaaatcataa gttgagacga taccccaact ttcatttact
     8761 tgcattactg catttagata atttagttgg ggtactcatt tataataatt ttaaatttat
     8821 attttcaatg aattgatgaa aatcatttaa tggagggcga gtatgggtaa agaatatgta
     8881 gtcatcggtc taggtcgttt tggaggtagt atcgttcgtg agttgaatgc attagacatg
     8941 gatgtaatgg ccatcgacca tgatgaaaat agagtgaatg aatatagtga tatcgctact
     9001 catgcggttg ttgcagacac tacagatgaa gcagttatga aaagtttagg tatccgtaac
     9061 tttgatcatg tcattgtggc aattggtgaa aatattcaat caagtacgtt gacgacttta
     9121 attttaaaag agttaggtgt aaaaaaagta actgctaaag cacaaaatga ttatcatgca
     9181 aagattttaa ataaaattgg agcagatacg gttgtgcacc ctgagcgtga tatgggtaga
     9241 cgtattgcgc ataatgttgc gagtgcaagt gtacttgatt atcttgagtt ggcagacgag
     9301 cattctattg tagaattgaa agcaactgaa aagatggcgg ggcagtctat cattgattta
     9361 gatataagag cacaatatgg aattaacatt attgcaatta aacgaggcaa agagttcatc
     9421 atttcaccaa atccaaatat taatttagaa ataggtgata ttttaatcat gattggacat
     9481 gataatgatt taaatcgctt tgaaaaaaat attgcgacga gataatcggg agtgggacag
     9541 aaatgatatt ttctcaaaat ttatttcgtc gtcccacccc aacttacatt gtctgtagaa
     9601 attgggaatc caatttctct ttgttggggc ccacacccca actcgcattg cctgtagaat
     9661 ttcttttcga aattctctat gttggggccc ctgactagaa ttgaaaaaag cttgttacaa
     9721 gcgcattttc gttcagtcaa ctactgccaa tataactttg tagagcattg aacattgatt
     9781 tatgtctcaa gctcatcata tataaagaaa aagctactaa caatgggtgg tgagtttaat
     9841 taaaacttca cttcaaagtt agtagctttt tgattattta ttgtttgatt ctttttgttc
     9901 gtttaccttc ataatgactg gtaaaatcat tggttttcta gctgtttttt caaataaata
     9961 aggttgtaat gtttcaatga tagaagattt aatctgatgc cattgaatat ctttattttg
    10021 atttaactta ctaataacat cagttttgat tttgcgttgt gcatcataaa ttaattgacc
    10081 tgattccctc atatatacaa atcctcgaga aataatgtct ggaccagaaa gtaatttatt
    10141 tgtattaaaa tcaatactaa caacaacgat aactaaacct tcttcagata atagcttacg
    10201 gtctcttatt acaacattac cgatatcacc gataccacta ccatcaacaa gtacattacc
    10261 agatggaatg cgaccagctt tacgtgctga atcgtgtgtt aaagctaaga catctccaat
    10321 atcaaagatg aagacattat cttcttcaac gccgcattca acaccagtct caccatgtgc
    10381 ttttaacata cggtattcac catgaatagg taagaaatat ttcggcttga ttaatcgaag
    10441 cattaattgt tgatcacctt gagaaccatg ccctgaagta tggatgttag aaatcttgct
    10501 atggataaca tctgcaccag ctttatacaa ggaattaata gttctgttaa tactttttgt
    10561 attacctggg ataggtgatg aactaaatac aacggtatct tcaggtataa ttttaatttg
    10621 cttatgagta ccattagcaa ttctagataa tgctgccatt ggttcacctt gtgaaccagt
    10681 acatagtatc aataactcat gcttcggtac ggtattaatt ttattaggtt caataaatgt
    10741 ttcaggtggt gctttaatat aaccaagttc catacctatt ttaatattgt tttccatcga
    10801 acgaccgaac gtaacaattt tacggttatt tttgatagca gcttcaactg cttgttgaac
    10861 tcggtaaata ttagaagcga aggtagcaaa tataatacga cctttacaat tacggaagat
    10921 cttatctacg ttttgaccaa cttcacgttc gcttaaagta aaatcaggca caagtgaatt
    10981 tgttgagtct gaaagtaaac atagaacgcc ttcttcgcct aattgagcca ttttagcaat
    11041 gtttgctggt ttgcctacag gtgtaaaatc aaatttaaag tcaccggtat gaactacttt
    11101 tccttcaggt gtatctacga tgacgccata agtttcagga atactatgtg tagttaagta
    11161 gaaagaaatc gtaaagtgct tagatttaat cacactgtcc tcattgattt catttagttt
    11221 agcagtacgt aataaatgat gttcttcaag tttattacgg attaaaccta atgctaaagg
    11281 accaccataa ataggtatat taagttgttt taataggaag ggcacaccgc ctatatggtc
    11341 ttcgtgacca tgtgttataa ataggccaac aattttatct tggttttgaa ctagatatgt
    11401 gtagtcaggt ataacataat caatccctaa taagttatca tcagggaatt tgatacctgc
    11461 atcgataatg acaatttcgt ctttatactc aactgcataa gtatttttac cgatttcacc
    11521 tagacctcca agtgcatata cacctacttc atttggatgt aattgtttca ttatttagca
    11581 atctccacat taaagtgttc tgagttttct ttttcgtaat ctaaatgtgc gccctctaat
    11641 ttagtgataa attcgatatt aaaattacga tctttcaagt aacgacgtac ttgttcttct
    11701 gtttgagctt caacataaag tgattgtgta ttttcacgca caattacctc gtctctgtta
    11761 tgttgataaa aaactttaaa tactgccatg ttaaaaattc ctcctaagaa tgtttgttta
    11821 atttattgtt aaccctagta aaatcgtatt ggagtatata tcgataaatt cattccaatc
    11881 atctctataa tgtaacttaa taacgatttg gttaataact aggttcatca tgtcgttcat
    11941 tttaaaaaat tagtgaaata acactaaaat ttcagttaaa tcttatttta catgatgaat
    12001 gataataaat aaagcagttt atctcactag aaatctaagt atttcgaata aatgtgcagt
    12061 tctatttatt aaaataaatg tagagattat aaattaatgt taagaaagaa ttaaaggatt
    12121 ttatatgaaa cggtaaaatt taaatgaggg gataaaaagt ttggtagaaa ggtgaatata
    12181 tttaactgtt gtttattcga aaaagttgta aaaggaaaag cttaggacat catatttatc
    12241 ctaagctttg ataattagaa aaatgtgttt taaacttcta ctgcatctgt atgtggttgt
    12301 aatgggtgat ctttgtcaat gtgatcatag aacattacac catttaaatg gtcaatttca
    12361 tgttggaaaa caattgctgg atatcctttt agtcgtaatt gtatatcatt accttcgatg
    12421 tctttggctt taattgtaat tctattatga cggtgaacta gaccagcaac attatcatcg
    12481 acactaaggc aaccttcacc agttggtaaa taagcttctt gaacgctatg acttacaatt
    12541 tttgggttca caagcatata gtcataagat ttgccactgc catcatctgg tattaaaaca
    12601 gcaatcatac gtttagaaat attaatttga ggtgcagcca aaccaacgcc tgaacgtaaa
    12661 ccatatcgtt tcgcgatttc ctcatcttga ctatttacta aaaactctct catggcgatt
    12721 aatgtttctt tttcttcttt agttaatggt aattctaact cagctgcttt ttgacgcaaa
    12781 gttggatgac catctctaat gatgtctttc attgttaaca tatattgcac cttccttatt
    12841 ttaatttgtt ttagttgaat gacagtaaaa aggttgttaa gatactcata catttttatg
    12901 tgtaaatatc tacaaagtta accaactact gccaatgttt attttagata gtatatgtaa
    12961 attttcagat atgctaattg cttaaaaaat gattaaagtg ttggtttcaa gcaatgatac
    13021 tttagaaatt tatttatcat cttgacttta aaaattatat tataaatgac gtaactgtca
    13081 acagatatac ttagtaatga agatgtgtaa tgtaattgtt taaaattgat ttccaagcag
    13141 attttattta tcatttaatt taaatagcaa gtggaggtac aagtaatgaa atttggaaaa
    13201 acaatcgcag tagtattagc atctagtgtc ttgcttgcag gatgtactac ggataaaaaa
    13261 gaaattaagg catatttaaa gcaagtggat aaaattaaag atgatgaaga accaattaaa
    13321 actgttggta agaaaattgc tgaattagat gagaaaaaga aaaaattaac tgaagatgtc
    13381 aatagtaaag atacagcagt tcgcggtaaa gcagtaaagg atttaattaa aaatgccgat
    13441 gatcgtctaa aggaatttga aaaagaagaa gacgcaatta agaagtctga acaagacttt
    13501 aagaaagcaa aaagtcacgt tgataacatt gataatgatg ttaaacgtaa agaagtaaaa
    13561 caattagatg atgtattaaa agaaaaatat aagttacaca gtgattacgc gaaagcatat
    13621 aaaaaggctg taaactcaga gaaaacatta tttaaatatt taaatcaaaa tgacgcgaca
    13681 caacaaggtg ttaacgaaaa atcaaaagca atagaacaga actataaaaa gttaaaagaa
    13741 gtatcagata agtatacaaa agtactaaat aaggttggta aagaaaagca agacgttgat
    13801 caatttaaat aattaatata atacagatgg taggaaacaa ctaatacagt tcctattatc
    13861 tgtatctttt tttattaaaa cagaactttt tcaaatggtt taacagtccc atttatttgt
    13921 ggtacaatta gtaaggataa aatgaatttc tatacaatta tgggaaaggt atggtgaatt
    13981 gaatggctcc taagttacaa gcccaattcg atgcagtaaa agttttaaat gatactcaat
    14041 cgaaatttga aatggttcaa attttggatg agaatggtaa cgtcgtaaat gaagacttag
    14101 tacctgatct tacggatgaa caattagtgg aattaatgga aagaatggta tggactcgta
    14161 tccttgatca acgttctatc tcattaaaca gacaaggacg tttaggtttc tatgcaccaa
    14221 ctgctggtca agaagcatca caattagcgt cacaatacgc tttagaaaaa gaagattaca
    14281 ttttaccggg atacagagat gttcctcaaa ttatttggca tggtttacca ttaactgaag
    14341 ctttcttatt ctcaagaggt cacttcaaag gaaatcaatt ccctgaaggc gttaatgcat
    14401 taagcccaca aattattatc ggtgcacaat acattcaagc tgctggtgtt gcatttgcac
    14461 ttaaaaaacg tggtaaaaat gcagttgcaa tcacttacac tggtgacggt ggttcttcac
    14521 aaggtgattt ctacgaaggt attaactttg cagcagctta taaagcacct gcaattttcg
    14581 ttattcaaaa caataactat gcaatttcaa caccaagaag caagcaaact gctgctgaaa
    14641 cattagctca aaaagcaatt gctgtaggta ttcctggtat ccaagttgat ggtatggatg
    14701 cgttagctgt atatcaagca actaaagaag cacgtgaccg cgcagttgca ggtgaaggtc
    14761 caacattaat tgaaactatg acatatcgtt atggtcctca tacaatggct ggtgacgatc
    14821 caactcgtta cagaacttca gacgaagatg ctgaatggga gaaaaaagac ccattagtac
    14881 gtttccgtaa attccttgaa aacaaaggtt tatggaatga agacaaagaa aatgaagtta
    14941 ttgaacgtgc aaaagctgat attaaagcag caattaaaga ggctgataac actgaaaaac
    15001 aaactgttac ttctctaatg gaaattatgt atgaagatat gcctcaaaac ttagcagaac
    15061 aatatgaaat ttacaaagag aaggagtcga agtaagccat ggcacaaatg acaatggttc
    15121 aagcgattaa tgatgcgctt aaaactgaac ttaaaaatga ccaagatgtt ttaatttttg
    15181 gtgaagacgt tggtgttaac ggcggtgttt tccgtgttac tgaaggacta caaaaagaat
    15241 ttggtgaaga tagagtattc gatacacctt tagctgaatc aggtattggt ggtttagcga
    15301 tgggtcttgc agttgaagga ttccgtccgg ttatggaagt acaattctta ggtttcgtat
    15361 tcgaagtatt tgatgcgatt gctggacaaa ttgcacgtac tcgtttccgt tcaggcggta
    15421 ctaaaactgc acctgtaaca attcgtagcc catttggtgg tggcgtacac acaccagaat
    15481 tacacgcaga taacttagaa ggtattttag ctcaatctcc aggtctaaag gttgttattc
    15541 cttcaggccc atacgatgcg aaaggtttat taatttcttc tattagaagt aatgacccag
    15601 tcgtatactt agagcatatg aaattgtatc gttcattccg tgaagaagta cctgaagaag
    15661 aatatacaat tgacattggt aaggctaatg tgaaaaaaga aggtaatgac atttcaatca
    15721 tcacatacgg tgcaatggtt caagaatcaa tgaaagctgc agaagaactt gaaaaagatg
    15781 gttattctgt tgaagtaatt gacttacgta ctgttcaacc aatcgatgtt gacacaattg
    15841 tagcttcagt tgaaaaaact ggtcgtgcag ttgtagttca agaagcacaa cgtcaagctg
    15901 gtgttggtgc agcagttgta gctgaattaa gtgaacgtgc aatcctttca ttagaagcac
    15961 ctattggaag agttgcagca gcagatacaa tttatccatt cactcaagct gaaaatgttt
    16021 ggttaccaaa caaaaatgac atcatcgaaa aagcaaaaga aactttagaa ttttaataca
    16081 ttttaaaagt taacgaagtt agcgtatttt agtctcattg attaaaatga aatgtttaat
    16141 ttacgaaatc ttaggagggc aaaaacgtgg catttgaatt tagattaccc gatatcgggg
    16201 aaggtatcca cgaaggtgaa attgtaaaat ggtttgttaa agctggagat actattgaag
    16261 aagacgatgt tttagctgag gtacaaaacg ataaatcagt agtagaaatc ccatcaccag
    16321 tatctggtac tgtagaagaa gttatggtag aagaaggtac agtagctgta gttggtgacg
    16381 ttattgttaa aatcgatgca cctgatgcag aagatatgca atttaaaggt catgatgatg
    16441 attcatcatc taaagaagaa cctgcgaaag aggaagcgcc agcagagcaa gcacctgtag
    16501 ctactcaaac tgaagaagta gatgaaaaca gaactgttaa agcaatgcct tcagtacgta
    16561 aatacgcacg tgaaaaaggt gttaacatta aagcagtttc tggatctggt aaaaatggtc
    16621 gtattacaaa agaagatgta gatgcatact taaatggtgg tgcaccaaca gcttcaaatg
    16681 aatcagctgc ttcagctaca agtgaagaag ttgctgaaac tcctgcagca cctgcagcag
    16741 taacattaga aggcgacttc ccagaaacaa ctgaaaaaat ccctgctatg cgtagagcaa
    16801 ttgcgaaagc aatggttaac tctaagcata ctgcacctca tgtaacatta atggatgaaa
    16861 ttgatgttca agcattatgg gatcaccgta agaaatttaa agaaatcgca gctgaacaag
    16921 gtactaagtt aacattctta ccttatgttg ttaaagcact tgtttctgca ttgaaaaaat
    16981 acccagcact taacacttca ttcaatgaag aagttggtga aatcgttcat aaacattact
    17041 ggaatatcgg tattgcagca gacactgata gaggattatt agtacctgtt gttaaacatg
    17101 ctgatcgtaa gtctattttc caaatttcag atgaaattaa tgaattagct gttaaagcac
    17161 gtgatggtaa attaacagcc gatgaaatga aaggtgctac atgcacaatc agtaatatcg
    17221 gttcagctgg tggacaatgg ttcactccag ttatcaatca cccagaagta gcaatcttag
    17281 gaattggccg tattgctcaa aaacctatcg ttaaagatgg agaaattgtt gcagcaccag
    17341 tattagcatt atcattaagc tttgaccaca gacaaattga tggtgcaact ggccaaaatg
    17401 caatgaatca cattaaacgt ttattaaata atccagaatt attattaatg gaggggtaaa
    17461 acatggtagt tggagatttc ccaattgaaa cagatactat agtaatcgga gcaggtcctg
    17521 gtggatacgt tgcagcaatt cgtgcagctc aattaggaca aaaagtaaca atcgttgaga
    17581 aaggtaatct tggtggtgtt tgcttaaacg taggatgtat tccttcaaaa gcattactac
    17641 atgcttctca ccgttttgtt gaagcacaac attctgaaaa cttaggtgtt attgctgaaa
    17701 gtgtttcttt aaacttccaa aaagttcaag aattcaaatc atcagttgtt aataaattaa
    17761 ctggtggtgt tgaaggctta cttaaaggta acaaagttaa catcgttaaa ggtgaagcat
    17821 atttcgtaga taacaatagc ttacgtgtta tggacgaaaa gagcgcacaa acatacaact
    17881 ttaaaaatgc aatcattgca acaggttcaa gaccaattga aattcctaat ttcaaattcg
    17941 gtaaacgtgt tatcgactca acaggtgctt taaacttaca agaagtacca ggtaaattag
    18001 ttgtagttgg tggaggatac attggatcag aattaggtac agcatttgct aactttggtt
    18061 cagaagtaac catccttgaa ggtgctaaag atatcttagg tggcttcgaa aaacaaatga
    18121 cacaacctgt taaaaaaggt atgaaagaaa aaggtgttga aatcgttact gaagctatgg
    18181 ctaaatcagc tgaagaaaca gataacggag ttaaagttac ttatgaagct aaaggcgaag
    18241 agaaaacaat cgaagctgat tatgtattag taactgtagg tcgtcgtcca aacacagacg
    18301 aattaggcct agaagaatta ggtgttaaat tcgctgaccg tggattatta gaagttgata
    18361 aacaaagccg tacgtctatc agcaatatct atgcaattgg tgatatcgtt ccaggtttac
    18421 cacttgctca caaagctagc tatgaagcta aagttgctgc tgaagcaatt gatggtcaag
    18481 ctgctgaagt tgattacatt ggtatgccag cagtatgctt tactgaacca gaattagcta
    18541 cagttggtta ttcagaagcg caagctaaag aagaaggttt agcaattaaa gcttctaaat
    18601 tcccatatgc agcaaatggt cgtgcattat cattagatga tactaacgga tttgttaaac
    18661 ttattacact taaagaagat gatactttaa tcggtgctca agtagttggt actggtgcat
    18721 cagatattat ctctgaatta ggtttagcaa ttgaagctgg tatgaatgct gaagatatcg
    18781 cattaacaat ccatgcacat ccaacattag gtgagatgac tatggaagca gcagaaaaag
    18841 ctatcggata cccaatccat acaatgtaat aactgattat ctataaagat tcagtcatta
    18901 aaagctgtag catatgctac ggcttttttg ttttaggtaa agtaatgtaa ggaaattgat
    18961 ttgagatatc gttaacatgt gacatgcatg ttatactagc gatgctaaca aaagaattga
    19021 aatggagggt tcaacaatgg aatatgagta tccaattgat ttagactgga gtaatgaaga
    19081 gatgatttca gtgataaatt tctttaatca tgtagagaag tattatgaat ccggcgtgac
    19141 ggcaggcgac tttatgggtg catataaaag atttaaagaa attgtgcctg ctaaagcaga
    19201 ggaaaaacaa atttttaata ctttcgaaaa aagtagtggc tataatagtt acaaagcagt
    19261 tcaagatgta aaaactcact ctgaagaaca aagagtaaca gctaaaaaat aattcgttcg
    19321 aaattaacac aatttaatag gaatttttct ttaaaactat tgctaataaa gctatatttt
    19381 gataccttta tcaagtgtta aacaaaatgt ttgataaaag taaacttaat atagcttttt
    19441 taggtggaaa aataaatgaa cataggtaat aaaattaaaa atcttagaag aattaaaaat
    19501 ttaacgcaag aagaacttgc tgaacgtaca gacttatcga aaggctacat ttcacaaata
    19561 gaaagtgaac atgcctcacc aagtatggaa actttcttaa atattataga ggtgttagga
    19621 acgacgccaa gtgaattttt taaagacagt gaaaatgaaa aagtattata caagaaggaa
    19681 gaacaagtta tttatgatga gtatgatgaa ggttatatat taaattggtt agtttcaaag
    19741 tcaaatgaat atgatatgga gccattaata ttaactttaa agcctggagc atcatataaa
    19801 aattttaatc catcagagtc tgatacgttt atttattgta tgtcaggtca gataacactt
    19861 aatttaggca aagagatata tcaagcacaa gaagaagacg ttttgtattt taaagcacga
    19921 gataatcatc gtttgtcaaa cgaatcaaac aatgaaacac gaatacttat tgtagcgaca
    19981 gcttcatatt tatagggggg atcttatttg gaaccgttat tatcattaaa atcagttagt
    20041 aaaagctatg atgatcttaa tatcttagat gacatagata ttgatattga atcaggatac
    20101 ttttatacat tattaggtcc ttcaggttgt ggtaaaacaa caattttaaa attaattgca
    20161 gggtttgaat atcctgacag tggtgaagtg atttatcaaa acaaaccaat tggtaattta
    20221 ccaccaaata aacgtaaagt gaatacagtc tttcaagatt atgcattatt tccacactta
    20281 aacgtctatg ataatatcgc ttttggtttg aaattaaaaa aattatcaaa aaccgaaatt
    20341 gatcaaaaag taactgaggc attaaaatta gtaaaacttt caggttatga aaaaagaaat
    20401 attaatgaaa tgagtggcgg acaaaagcaa cgtgttgcaa ttgcacgtgc tatcgtaaat
    20461 gaaccagaaa tattattgtt agatgaatct ttatccgcat tagatttgaa attgcgtact
    20521 gaaatgcaat atgaattacg agaattgcaa tctagattag gtattacatt tatatttgta
    20581 acacatgatc aagaagaagc gttagcatta agtgactttc tttttgtatt aaaagatggg
    20641 aaaattcaac aatttggcac accaacagat atatatgacg aaccagtgaa tcgatttgta
    20701 gctgatttta ttggagaatc taatattgtt gaagggcgca tggttagaga ttatgtcgtg
    20761 aatatttata ggcaagattt cgaatgtgtc gatatgggta ttcctgaaaa taaaaaagta
    20821 gaagtcgtta ttcgaccaga agatatatca ttaatcaaag ctgaagaagg attatttaaa
    20881 gcaactgttg attctatgtt atttagaggg gtccactatg aaatatgttg tatagacaat
    20941 aaaggttatg aatgggtaat acaaacgact aaaaaagctg aagtaggcag tgaagttggt
    21001 ctttattttg atcctgaagc cattcatatt atggttcctg gagaaacaga agaagaattt
    21061 gataaacgta ttgaaagcta tgaggaagta gacaatgcgt aatactaata aatttctctt
    21121 aatcccgtat ttactatgga tggttatatt tattattgta ccagttgtat tactcattta
    21181 tttttcattt ttagatatca atggacattt tagtttcacg aattatcaac aaatttttac
    21241 tacaaaatat ttgaaaatgt ttgcatattc aattttatat gccgcttcaa taacaattat
    21301 taccttgact atcagttatc cagctgccta ttatattact cgttcgaaat ttcaaaatat
    21361 cttattaatg ataatgatta ttccaacatg gataaattta ttgttaaaga catatgcttt
    21421 tataggttta ttaagtcatg atggcgtgat taatcaattt ttccacttat ttaatttacc
    21481 atcattcaat ttgttattta caactggtgc gtttttagta gtggcaagtt acatttatat
    21541 accatttatg attttaccta tatttaatag catgaaagca attcctaata atttattgca
    21601 ggcctcaagt gatttaggtg ccagtccttt ctatactttt agaaaagtaa tcatgccgtt
    21661 aacaaaagaa ggtgttatga ctgggattca agtaaccttt attccatcac tttcattatt
    21721 tatgattaca agattaattg caggtaataa agtcataaat ataggtacgg caatagagga
    21781 acaattttta acaattcaaa attatggtat gggatcaact atagctatat tcttaattgt
    21841 atttatggca ttcattttaa tcattacaaa atcatctaat gggagagggt gaatcatatg
    21901 aaatggtatg gaaagctgta tatcgggata cttttagcga ttttatacat cccaatattc
    21961 tttttaatgt tctattcatt caattcggct ggtaatatga ttcactttga acattttaca
    22021 ttagagcatt atcaatcatt atttcaaaat gatcatttaa tgtcggtcat ttttaatacg
    22081 atagctgtag cacttttagc agcctcaatt tctacagtta ttggtacatt tggtgccatt
    22141 gctatttatt atttaagaaa taaaaagttt aaagtaactt tactaacatt gaataatgtc
    22201 ttgatggtat catccgacgt tgtcataggt gcatcattct taattatgtt tacaacgatt
    22261 ggccatttta ctggtcttgg tttaggattt tggacagttc taatatctca tattgcattt
    22321 tgcataccta tagttgtgat tatcgtctta ccacaactgt atgaaatgaa taataatatg
    22381 ttaaatgctg caagagattt aggagcgact gaaccacaat tattaagcaa cattattatt
    22441 cctaatattt taccttctat tataggagga ttctttatgg ctttaactta ttcactagac
    22501 gatttcacag taagtttctt cgttactggt aatggcttta gtgtgttatc agttgaagtt
    22561 tatgctatgg cgagaaaagg aattagtatg gaaattaatg cgatttcaac attattgttt
    22621 gctgttattg tattaggaat actaggatat tatttgattc aatacgtgat aaataagaaa
    22681 aaactaatca agcgaggtgt aaaataatga aacgattttt acaactcatt ataggtgcat
    22741 tagttgtggg tatgctttgt cttactttaa gtcattggtt taaatctaaa gaacaagtgc
    22801 atacaaatca aaaaatttac gtatacaatt ggggcgaata tattgatcca gagttaatta
    22861 agaaatttga aaaagaaact ggcattcaag tcgtttatga aactttcgat tcaaatgaag
    22921 cgatggaagc caaaattcgc aatggcggta cacattatga tgttgctttt cctagtgaat
    22981 atacagttca aaaattgaaa agagatcatt tattgttacc aatagatcat aataaggtac
    23041 ctaatattaa aaatttagat tcagattata tgaatatgtc atttgataga ggcaataaat
    23101 attctttacc ttatttcttt ggaactgtag gtattttata taataaagaa aagtatccaa
    23161 atgaatcatt tgatagttgg aagtcattgt ataatcctaa atttaaaaac caaattttac
    23221 tagttgacgg tgctagagag attataggca tgagtttgaa taaacttggg tataatctta
    23281 atgaccgtaa ttcgcaccat ttaaaagaag cagagcgaga tttaaccaaa ctagcaccac
    23341 aagtaagagg tgtcgtaggt gatgaaatta ccatgatgct tcaacaaaat gaaggtaaca
    23401 tagcggttgt ttggagtggt gttgcagcac ctctagtgca agaaggggat aaatataatt
    23461 atgttattcc taaagaagga tcgaatttat ggttcgacaa tatggtaatt ccaaaaacgg
    23521 cacaaaataa agagggtgca tataaattta tgaatttttt attagatgct aaaaataaca
    23581 agcaaaatac agaattcgta ggctatgcaa cgccaaacaa ggctgctcga caattgttgc
    23641 ctaaagagat taaagacgac catcgttttt atccgactaa gaaagagcaa gaacgccttg
    23701 aagtttataa agatttagga cctgaagttt taagtgaata caatgaaaac tttttgaatt
    23761 tcaaaatgtc attaaaataa aatcataaat agtagttatt tactgtatat ttagttataa
    23821 taacgggata agtactataa ggagttgtaa cgaatgactg gagaacaatt tactcaaatt
    23881 aaacgtccag taagtagatt aactgaaaaa gttctaggtt ggttatgttg ggtgatgtta
    23941 ttagtgctta ctgtcattac gatgtttata gcacttgttt cattcagtaa taacacatcg
    24001 attgctaatc ttgaaaatac attaaacaat aatgcattta tccagcaatt attagctgga
    24061 aatggctata atacaacaca atttgtaata tggttacaaa atggtatatg ggctattatc
    24121 gtttacttta ttgtttgttt gttgatttca tttttagctc tcatatctat gaatataaga
    24181 atcttgtcag gtttcttatt cttaatatca gcaattgtaa cgattccttt agttttactt
    24241 atcgttactt taatcattcc gatattattc tttattattg cgatgatgct atttataaga
    24301 aaagataaag ttgaaatggt tgcgccacaa tattatgaag agtataacgg accgatttat
    24361 gattatcgtg aacctgtgta tgagcgcctc caaccgaaag atgattatta tgatgtgcct
    24421 aaatatgaaa aagaattgga taaatcaaat actgtatatg atcaagaaca ggaaagagat
    24481 aaatatgatc aatttcctaa acgtgcagtt gaaagtgaat ataatcatga tgagcgcact
    24541 gaggaagaac catcagtatt atccagacag gctaaataca aacaaaaaag tactgaagaa
    24601 ctaggtattg aagatgatgg ttattatgca gaacctgaag ttgatccaaa agaattgaaa
    24661 gcacaacaaa agcgagaaaa agctgaaatt aaagctaaga aaaaagaaaa acgtaaagct
    24721 tataaccaac gaatgaaaga acgtaggaaa aatcaaccta gcgcagttag tcaacgtcga
    24781 atgaattttg aagagcgacg tcaaatttac aacaatgata tttctgaaga acgcaattca
    24841 agtgaagtta aggacaaaaa agagcaagaa taaatattga tgatacccag attagtaagc
    24901 agaggtcttt tgcttattaa tctgggtttt tatatgaggt taattatcga taacgtttaa
    24961 ttaaagtgtt taggtgtcat aattttaaat gacgatttct cattactata cacctaaatt
    25021 atcatcaatc tgaattcaga tgtttattat aaaaattaga tgaaaaatat gttaatatac
    25081 aagtaattta atgtgcgtat atcta
//
LOCUS       DAXWFK010000018        24812 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.018, whole genome shotgun sequence.
ACCESSION   DAXWFK010000018 DAXWFK010000000
VERSION     DAXWFK010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 24812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24812
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.018"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(706..1563)
                     /locus_tag="ACGEXS_000607"
     CDS             complement(706..1563)
                     /locus_tag="ACGEXS_000607"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFF8011654.1"
                     /translation="MDNVKAIFLDMDGTILHENNQASTYTKDVINQLREKGYKVFLAT
                     GRSHSEIHQLVPQDFAVNGIISSNGTIGEVDGEIIFKHGLSLAQVQQITNLAKRQQIY
                     YEVFPFEGNRVSLKEDETWMRDMIRSQDPINGVSHSEWSSRQDALAGKIDWVTKFPEG
                     EYSKIYLFSSNLEKITAFRDELKQNHVQLQISVSNSSRFNAETMAYQTDKGTGIKEMI
                     AHFGIHQEETLVIGDSDNDRAMFEFGHYTVAMKNARPEIQALTSDVTAYTNEEDGAAK
                     YLAEHFLAE"
     gene            complement(1631..2413)
                     /locus_tag="ACGEXS_000608"
     CDS             complement(1631..2413)
                     /locus_tag="ACGEXS_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8011655.1"
                     /translation="MLIQLDQIGRMKQGKTILKKISWQIAKGDKWILYGLNGAGKTTL
                     LNILNAYEPATSGTVNLFGKMPGKVGYSAETVRQHIGFVSHSLLEKFQEGERVIDVVI
                     SGAFKSIGVYQDIDDEIRNEAHQLLKLVGMSAKAQQYIGYLSTGEKQRVMIARALMGQ
                     PQVLILDEPAAGLDFIARESLLSILDSLSDSYPTLAMIYVTHFIEEITANFSKILLLK
                     DGQSIQQGAVEDILTSENMSRFFQKNVAVQRWNNRFSMAMLE"
     gene            complement(2645..4450)
                     /gene="glmS"
                     /locus_tag="ACGEXS_000609"
     CDS             complement(2645..4450)
                     /gene="glmS"
                     /locus_tag="ACGEXS_000609"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFF8011656.1"
                     /translation="MCGIVGYIGYDNAKELLLKGLEKLEYRGYDSAGIAVVNDDNTTV
                     FKEKGRIAELRKVADSSDFDGPVGIGHTRWATHGVPNHENSHPHQSSNGRFTLVHNGV
                     IENYEELKGEYLQGVSFISETDTEVIVQLVEYFSNQGLSTEEAFTKVVSLLHGSYALG
                     LLDAEDKDTIYVAKNKSPLLLGVGEGFNVIASDALAMLQVTSEYKEIHDHEIVIVKKD
                     EVIIKDADGNVVERDSYIAEIDASDAEKGVYAHYMLKEIHEQPAVMRRIIQEYQDAEG
                     NLKIDQDIINDVKEADRIYVIAAGTSYHAGLVGKEFLEKWAGVPTEVHVASEFVYNMP
                     LLSEKPLFVYISQSGETADSRAVLVETNKLGHKSLTITNVAGSTLSREADHTLLLHAG
                     PEIAVASTKAYTAQIAVLSILSQIVAKEHGREADIDLLRELAKVTTAIEAIVDDAPIM
                     EQIATDFLETTRNAFFIGRTIDYNVSLEGALKLKEISYIQAEGFAGGELKHGTIALIE
                     EGTPVVGLATQEKVNLSIRGNVKEVVARGAHPCIISMEGLEKEGDTYVIPHVHELLTP
                     LVSVVALQLISYYAALHRDLDVDKPRNLAKSVTVE"
     gene            5131..6669
                     /locus_tag="ACGEXS_000610"
     CDS             5131..6669
                     /locus_tag="ACGEXS_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFF8011657.1"
                     /translation="MSQTEEKKGIGRRVQAFGSFLSSMIMPNIGAFIAWGFIAAIFID
                     NGWLPNKDLATLAGPMITYLIPLLIAFSGGRLIYDLRGGIIAATATMGVIVALPDTPM
                     LLGAMIMGPLVGWLMKKTDQLIQPRTPQGFEMLFNNFSAGILGFIMTIAGFKILAPLM
                     KFIMHILSVAVEALVHAHLLPLVSILVEPAKIVFLNNAINHGVFTPLGADQAAKAGQS
                     ILYTIESNPGPGLGILLAYMIFGKGTAKATSYGAGIIHFLGGIHEIYFPYVLMRPLLF
                     IAVILGGMTGVATYQATGFGFKSPASPGSFIVYCLNAPRGEFLHMLLGVFLAALVSFV
                     VAALIMKFTREPKQDLEAATAQMENTKGKKSSVASKLVSSDKNVNTEENASGNVSETS
                     SSDDDPEALLDNYNTEDVDAHNYNNINHVIFACDAGMGSSAMGASMLRNKFKKAGIND
                     ITVTNTAINQLPKDAQLVITQKKLTDRAIKQTPNAIHISVDNFLNSPRYEELLNNLKK
                     DDQA"
     gene            6704..8836
                     /locus_tag="ACGEXS_000611"
     CDS             6704..8836
                     /locus_tag="ACGEXS_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFF8011658.1"
                     /translation="MLLSTREKEMIALLIKYHGQYITIHDIAQQLAVSSRTIHRELKG
                     VEAYLTSFSLTLERANKKGLRIAGTDSDLNDLKQSIAQHQTIDLSVEEQKVIIIYALI
                     QAKEPVKQYSLAQEIGVSVQTLAKMLDDLELDLNKYQLSLSRKRGEGIYLVGTESKKR
                     EFLSQLMVNNLNSTSVYSVIENHFVFHSLNQIHKDFVDLERIFNVERLLMDYLSALPY
                     QLTESSYLTLTVHIVLSISRIKNGEYVALNDDIYDSVQNTFEHKVASELADKLGQIYD
                     VTFNQAEIAFITIHLRGAKRKNLNDTSLNNRCEENKIKAFVNKVEMISGMTFADLDTL
                     VDGLTLHLNPAINRLQANIETYNPLTDMIKFKYPRLFENVRLALNDCWPDLIFPESEI
                     AFIVLHFGGSIKNQGNRFLNILVVCSSGMGTSRLLSTRLEQVFSEIERITQASVSDLK
                     SLDLSQYDGIISTVNLDIDSPYLTVNPLLPDSDISYVAQFLNTKSTFQETHDKSSNMI
                     DKDDVHVETKDVDGNTSFENEQTSYLTSVFEKHLSDEKSEQLLHHMRSGLTLLDSVKI
                     VSTEVKQWQTYIADYLYQCDVINDPTSFAELLEQRLIDNPGWILSPYPVAIPHLRDNM
                     IKHPMILITVLEEPLTLPSIQNDNQTIKYMISMFISDNDFMASLVSDLSEFLSLKLES
                     IDTFMENPQELETLLRNKFLERIKKQFI"
     gene            8848..9282
                     /locus_tag="ACGEXS_000612"
     CDS             8848..9282
                     /locus_tag="ACGEXS_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8011659.1"
                     /translation="MSELFSNDNIFLNVNVNSQNEAIEKAGKALVDSGAVTDAYIQAM
                     KDREQVVSTFMGNGLAIPHGTDEAKTNVIHSGLTLLQIPEGVDWDGEVVKVVVGIAGK
                     DGEHLDLLSKIAITFSEEENVDRIVQAKSAEEIKQVFEEADA"
     gene            9282..10388
                     /locus_tag="ACGEXS_000613"
     CDS             9282..10388
                     /locus_tag="ACGEXS_000613"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFF8011660.1"
                     /translation="MKAVHFGAGNIGRGFIGYILADNNVKVTFADVNEEIINALAHDH
                     QYDVILADESKTTTRVNNVDAINSMQPSEALKQAILEADIITTAVGVNILPIIAKSFA
                     PFLKEKTNHVNIVACENAIMATDTLKKAVLDITGPLGNNIHFANSAVDRIVPLQKNEN
                     ILDVMVEPFYEWVVEKDAWYGPELNHIKYVDDLTPYIERKLLTVNTGHAYLAYAGKFA
                     GKATVLDAVEDSSIEAGLRRVLAETSQYITNEFDFTEAEQAGYVEKIIDRFNNSYLSD
                     EVTRVGRGTLRKIGPKDRIIKPLTYLYNKDLERTGLLNTAALLLKYDDTADQETVEKN
                     NYIKEHGLKAFLSEYAKVDDGLADEIIEAYNSLS"
     gene            complement(10707..18143)
                     /gene="fmtB"
                     /locus_tag="ACGEXS_000614"
     CDS             complement(10707..18143)
                     /gene="fmtB"
                     /locus_tag="ACGEXS_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored DUF1542 repeat protein FmtB"
                     /protein_id="HFF8011661.1"
                     /translation="MNLFRQQKFSIRKFNVGIFSALIATVTFISTNPTTASAAEQNQP
                     AQNQPAQPADANTQPNANAGAQANPTAQPAAPANQGQPAVQPANQGGQANPAGGAAQP
                     NTQPAGQGNQADPNNAAQAQPGNQATPANQAGQGNNQATPNNNATPANQTQPANAPAA
                     AQPAAPVAANAQTQDPNASNTGEGSINTTLTFDDPAISTDENRQDPTVTVTDKVNGYS
                     LINNGKIGFVNSELRRSDMFDKNNPQNYQAKGNVAALGRVNANDSTDHGNFNGISKTV
                     NVKPDSELIINFTTMQTNSKQGATNLVIKDAKKNTELATVNVAKTGTAHLFKVPTDAD
                     RLDLQFIPDNTAVADASRITTNKDGYKYYSFIDNVGLFSGSHLYVKNRDLAPKATNNK
                     EYTINTEIGNNGNFGASLKADQFKYEVTLPQGVTYVNNSLTTTFPNGNEDSTVLKNMT
                     VNYDQNANKVTFTSQGVTTARGTHTKEVLFPDKSLKLSYKVNVANIDTPKNIDFNEKL
                     TYRTASDVVINNAQPEVTLTADPFSVAVEMNKDALQQQVNSQVDNSHYTTASIAEYNK
                     LKQQADTILNEDANHVKTANRASQADIDGLVTKLQAALIDNQAAIAELDTKAQEKVTA
                     AQQSKKVTQDEVAALVTKINNDKNNAIAEINKQTTAQGVTTEKDNGIAVLEQDVITPT
                     VKPQAKQDIIQAVTTRKQQIKKSNASLQDEKDVANDKIGKIETKAIKDIDAATTNAQV
                     EAIKTKAINDINQTTPATTAKAAALEEFDEVVQAQIDQAPLNPDTTNEEVAEAIERIN
                     AAKVSGVKAIEATTTAQDLERVKNEEISKIENITDSTQTKMDAYNEVKQAATARKAQN
                     ATVSNATNEEVAEADAAVDAAQKQGLHDIQVVKSKQEVADTKSKVLDKINAIQTQAKV
                     KPAADTEVENAYNTRKQEIQNSNASTTEEKQAAYTELDTKKQEARTNLDAANTNSDVT
                     TAKDNSIAAINQVQAATTKKSDAKAEIAQKASERKTAIEAMNDSTTEEQQAAKDKVDQ
                     AVVTANADIDNAAANNDVDNAKTTNEATIAAITPDANVKPAAKQAIADKVQAQETAID
                     GNNGSTTEEKAAAKQQVQTEKTTADAAIDAAHTNAEVEAAKKAAIAKIEAIQPATTTK
                     DNAKEAIATKANERKTAIAQTQDITAEEIAAANADVDNAVTQANSNIEAANSQNDVDQ
                     AKTTGENSIDQVTPTVNKKATARNEITAILNNKLQEIQATPDATDEEKQAADAEANTE
                     NGKANQAISAATTNAQVDEAKANAEAAINAVTPKVVKKQAAKDEIDQLQATQTNVINN
                     DQNATTEEKEAAIQQLATAVTDAKNNITAATDDNGVDQAKDAGKNSIQSTQPATAVKS
                     NAKNDVDQAVTTQNQAIDNTTGATTEEKNAAKDLVLKAKEKAYQDILNAQTTNDVTQI
                     KDQAVADIQGITADTTIKDVAKDELATKANEQKALIAQTADATTEEKEQANQQVDAQL
                     TQGNQNIENAQSIDDVNTAKDNAIQAIDPIQASTDVKTNARAELLTEMQNKITEILNN
                     NETTNEEKGNDIGPVRAAYEEGLNNINAATTTGDVTTAKDTAVQKVQQLHANPVKKPA
                     GKKELDQAAADKKTQIEQTPNASQQEINDAKQEVDTELNQAKTNVDQSSTNEYVDNAV
                     KEGKAKINAVKTFSEYKKDALAKIEDAYNAKVNEADNSNASTSSEIAEAKQKLAELKQ
                     TADQNVNQATSKDDIEVQIHNDLDNINDYTIPTGKKESATTDLYAYADQKKNNISADT
                     NATQDEKQQAIKQVDQNVQTALESINNGVDNGDVDDALTQGKAAIDAIQVDATVKPKA
                     NQAIEVKAEDTKESIDQSDQLTAEEKTEALAMIKQITDQAKQGITDATTTAEVEKAKA
                     QGLEAFDNIQIDSTEKQKAIEELETALDQIEAGVNVNADATTEEKEAFTNALEDILSK
                     ATEDISDQTTNAEIATVKNSALEQLKAQRINPEVKKNALEAIREVVNKQIEIIKNADA
                     DASAKEIARTDLGRYFDRFADKLDKTQTNAEVAELQNVTIPAIEAIVPQNDPDANDTN
                     NGIDNNDATANSNANATPENTGQPNVSETTANGKADASPTTPNNSDAATGETTATSAT
                     DDANDKPQANNNSSVDASTNSPTMDNDVTSKPEVESTNNGTTDKPVTETDNATPAEST
                     TNNNSTTTATNENAPTGSTATAPTTASTEAASSTDSKDNASVNDSKQNAEVNNSAESQ
                     STNDKVAQPKSENKAKAEKDGSDSTNQSMVESTTETLPSADITEPNVPSNTSKDKEES
                     TTNQTDAGQLKSETNVASNEADKSPSKADTEVSNKPSTSASSEAKEKMTSTNVSQKDD
                     TATADTNDTQKSVGSAANNKATQNDGANASPATVSNGSNSANQDMLNVTNTDDHQAKT
                     KSAQQGKVNKAKQQAKTLPDTGMSHNDDLPYAELALGAGMAFLIRRFTKKDQQTEE"
     gene            complement(18420..19775)
                     /gene="glmM"
                     /locus_tag="ACGEXS_000615"
     CDS             complement(18420..19775)
                     /gene="glmM"
                     /locus_tag="ACGEXS_000615"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFF8011662.1"
                     /translation="MGKYFGTDGVRGVANQELTPELAFKLGRYGGYVLAHNKGEKHPR
                     VLVGRDTRVSGEMLESALIAGLISIGAEVMRLGIISTPGVAYLTRDMGAELGVMISAS
                     HNPVADNGIKFFGSDGFKLSDEQENEIEALLDQENPELPRPVGNDIVHYSDYFEGAQK
                     YLSYLKSTVDVNFEGLKIALDGANGSTSSLAPFLFGDLEADTETIGCSPDGYNINEKC
                     GSTHPEKLAEKVVETESDFGLAFDGDGDRIIAVDENGQIVDGDQIMFIIGQEMHKNQE
                     LNNDMIVSTVMSNLGFYKALEQEGIKSNKTKVGDRYVVEEMRRGNYNLGGEQSGHIVM
                     MDYNTTGDGLLTGIQLASVIKMTGKSLSELAGQMKKYPQSLINVRVTDKYRVEENVDV
                     KEVMTKVEVEMNGEGRILVRPSGTEPLVRVMVEAATDEDAERFAQQIADVVQDKMGLD
                     K"
     gene            complement(19802..20734)
                     /locus_tag="ACGEXS_000616"
     CDS             complement(19802..20734)
                     /locus_tag="ACGEXS_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HFF8011663.1"
                     /translation="MLESKWGLRFIAFLLALFFFLSVNNVFGNIFNTGNLGQKSSKTI
                     QDVPVEILYNTKDLHLTKAPETVNVTISGPQSKIIKIENPEDLRVVIDLSNAKAGKYQ
                     EKYQVKGLADDIHYSVKPKLANITLENKVTKKMTVQPDVSQSDIDPLYKITKQEVSPQ
                     TVKVTGGEEQLNDIAYLKATFKTNKKINGDTKDVAEVTAFDKKLNKLNVSIQPNEVNL
                     QVKVEPFSKKVKVNVKQKGSLADDKELSSIDLEDKEIEIFGSRDDLQNISEVDAEVDL
                     DGISESTEKTVKINLPEHVTKAQPSETKAYINVK"
     gene            complement(20736..21545)
                     /gene="cdaA"
                     /locus_tag="ACGEXS_000617"
     CDS             complement(20736..21545)
                     /gene="cdaA"
                     /locus_tag="ACGEXS_000617"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFF8011664.1"
                     /translation="MDFSNFFQNLSTLKIVTSILDLLIVWYVLYLLITVFKGTKAIQL
                     LKGILVIVIGQQISMILNLTATSKLFDIVIQWGVLALIVIFQPEIRRALEQLGRGSFL
                     KRYTSNTYSKDEEKLIQSVSKAVQYMAKRRIGALIVFEKETGLQDYIETGIAMDSNIS
                     QELLINVFIPNTPLHDGAMIIQGTKIAAAASYLPLSDSPKISKSLGTRHRAAVGISEV
                     SDAFTVIVSEETGDISVTFDGKLRRDISNEIFEELLAEHWFGTRFQKKGVK"
     gene            complement(21734..22642)
                     /gene="rocF"
                     /locus_tag="ACGEXS_000618"
     CDS             complement(21734..22642)
                     /gene="rocF"
                     /locus_tag="ACGEXS_000618"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HFF8011665.1"
                     /translation="MTKTKAIDIIGAPSTFGQRKLGVDLGPTAIRYAGLISRLKQLDL
                     DVYDKGDIKVPAVNIEKFHSEQKGLRNYDEIIDVNQKLNKEVSASIENNRFPLVLGGD
                     HSIAVGSVSAISKHYNNLGVIWYDAHGDLNIPEESPSGNIHGMPLRILTGEGPKELLE
                     LNSNVIKPENIVLIGMRDLDKGERQFIKDHNIKTFTMSDIDKLGIKEVIENTIEYLKS
                     RNVDGVHLSLDVDALDPLETPGTGTRVLGGLSYRESHFALELLHQSHLISSMDLVEVN
                     PLIDSNNHTAEQAVSLVGTFFGETLL"
     gene            22868..24187
                     /locus_tag="ACGEXS_000619"
                     /pseudo
     CDS             22868..24187
                     /locus_tag="ACGEXS_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1181 family transposase"
     gene            24206..24310
                     /locus_tag="ACGEXS_000620"
     CDS             24206..24310
                     /locus_tag="ACGEXS_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011666.1"
                     /translation="MYFKNSSLNYIPTTFGGEPKKKHFPKMESASSEP"
     gene            complement(24301..24373)
                     /locus_tag="ACGEXS_000621"
     tRNA            complement(24301..24373)
                     /locus_tag="ACGEXS_000621"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24338..24340),aa:Lys,seq:ttt)
     gene            complement(24377..24451)
                     /locus_tag="ACGEXS_000622"
     tRNA            complement(24377..24451)
                     /locus_tag="ACGEXS_000622"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24417..24419),aa:Gln,seq:ttg)
     gene            complement(24461..24544)
                     /locus_tag="ACGEXS_000623"
     tRNA            complement(24461..24544)
                     /locus_tag="ACGEXS_000623"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24508..24510),aa:Tyr,seq:gta)
     gene            complement(24574..24649)
                     /locus_tag="ACGEXS_000624"
     tRNA            complement(24574..24649)
                     /locus_tag="ACGEXS_000624"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24614..24616),aa:Val,seq:tac)
     gene            complement(24657..24728)
                     /locus_tag="ACGEXS_000625"
     tRNA            complement(24657..24728)
                     /locus_tag="ACGEXS_000625"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24693..24695),aa:Glu,seq:ttc)
     gene            complement(24730..24804)
                     /locus_tag="ACGEXS_000626"
     tRNA            complement(24730..24804)
                     /locus_tag="ACGEXS_000626"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24770..24772),aa:Asn,seq:gtt)
BASE COUNT         7306 a         4407 c         3975 g         9124 t
ORIGIN      
        1 aaatgcaata aagttccaaa tacttattat tattcatcac aaattacgtg atgccctcta
       61 caacactaaa tcaaggatga tatttatttt atatacatta gcattcattg tttgcttacc
      121 cacattatcc tgtttagtct tttttataac tctagtcttg aatgtcatat ttacaaagaa
      181 aaaaacacta aaatatttaa aactaacact atttattcac tttgttcttt tatagcacct
      241 tttatgttta ttttcttgta ttcaataaat agtatggaga tgataattta aagtagatat
      301 atttgaagta tactatagag taaattatat tggggaaata tgtaaacgaa ccggttgatt
      361 ttatagtgga ttaacacttc atagagaata taatcaagaa caaaaacagt caataggtgt
      421 gaattttttt aaagacggat ctgttgctac taaaaaaaga agtatacaat tcaatagtta
      481 aaccttaaaa caagaaatat tattcaaaat caatgaattt cctatcttat tagttttaac
      541 aatatttatt ctcatagagt ttgtccaatt aaatatagat gattcaaata tttataagca
      601 tacaaaagag cagtaagaca ttttctaata gaaaataact ttactgctct ttgtacatta
      661 ctcatcaact attgtaaatt aaataataaa taactaccta ttttattatt cagctaaaaa
      721 atgctctgct aaatattttg ctgcgccatc ctcttcattc gtgtatgccg ttacatctga
      781 agttaatgct tggatttcag ggcgtgcatt tttcatagca actgtataat gaccaaattc
      841 aaacattgct ctatcattgt cgctatctcc aataactaac gtttcttctt gatgaatacc
      901 aaaatgtgca atcatttctt taatgcctgt acctttatca gtttgataag ccattgtttc
      961 cgcattaaat cttgatgaat ttgaaacact aatctgtagt tgcacatgat tttgctttaa
     1021 ttcatctcta aatgctgtta ttttttctaa attagaactg aatagataaa tttttgaata
     1081 ttcaccttca ggaaacttag ttacccaatc tatcttacca gcaagcgcat cttgtcttga
     1141 agaccattca ctatgactta cgccattaat aggatcttga ctacgaatca tatctcgcat
     1201 ccatgtttca tcttctttta aagaaactct attaccttca aaaggaaata cctcataata
     1261 aatttgttgg cgcttagcta aattagtaat ttgttgcact tgagccaatg ataaaccatg
     1321 cttgaagata atttctccat ctacttctcc aattgttcca tttgaactaa tgatgccatt
     1381 aaccgcaaaa tcttgaggta caagttgatg tatttcagaa tgcgaacgtc ctgttgccaa
     1441 aaatacttta tatcctttct ctctcaattg attaatgaca tctttcgtat acgttgatgc
     1501 ttgattattt tcatgtaaaa ttgttccatc catgtccaaa aatattgctt ttacgttatc
     1561 catattgccc tccactgtcc attttatctt aattaaattt tgtcattatt acttattatt
     1621 tgcaaaatat ttactctaac attgccatag aaaatcgatt attccatctt tgaactgcta
     1681 catttttctg gaaaaatcgt gacatgtttt cagaagttaa tatgtcttct acagcgcctt
     1741 gttgaatact ttggccatct tttagcagta aaattttgga aaagttagca gttatttctt
     1801 caataaagtg cgtcacataa atcatcgcaa gcgttggata tgaatctgac aatgagtcaa
     1861 gtatacttaa caacgattct cgtgcaataa agtctaaacc agctgctggc tcatctaaaa
     1921 ttaaaacctg gggttgcccc attaaagctc gtgcaatcat cactcgttgt ttttcaccgg
     1981 tagataaata accaatatat tgttgcgctt tagcagacat tccaactaat ttaagtaatt
     2041 gatgtgcttc attacgtatc tcatcatcaa tatcttgata aacaccaatt gatttaaagg
     2101 caccgcttat caccacatcg attactcttt caccctcttg aaacttttcc agtaaactat
     2161 gagatacaaa acctatatgt tgtcgtacag tctctgcaga ataccctacc ttgcctggca
     2221 ttttaccgaa aaggttaaca gttccagatg ttgcaggctc atacgcattt aaaatattta
     2281 gaagtgttgt cttgccagca ccattcaacc catataatat ccatttatca cctttagcaa
     2341 tttgccaaga aatctttttt aaaattgttt ttccttgctt cattcgccca atttgatcta
     2401 attgaattaa catgcgaggt caccccttta ttgctactcc atacttctca taaaatcata
     2461 ttaacataac acccttaatt gtcagactat ttaaataaat aaaacacttc atttttacgc
     2521 atttctgcca aattaagatg aagtaaaagc taagtcgacc taaaaaagca cccttctagt
     2581 cgattaatct aaaagggtgc catatacttt aattttaata catgattgat tctaaaaaag
     2641 tgaattattc cacagtaact gatttagcaa ggttacgtgg tttatcaaca tctaaatctc
     2701 tgtgtaatgc tgcatagtat gaaattaatt gtaatgcaac cactgatact aatggcgtta
     2761 acaattcatg tacatgagga atgacataag tgtcgccttc tttttcaaga ccctccatag
     2821 aaataataca tggatgtgca ccacgtgcta ctacctcttt aacgttacca cgaattgata
     2881 aattaacttt ctcttgtgtt gctaaaccta caactggtgt accttcttcg attaaggcaa
     2941 ttgtaccatg tttaagttct ccaccagcaa aaccttctgc ttgaatgtaa gaaatttctt
     3001 taagttttaa cgcaccttct aaacttacgt tatagtcaat agtacgtccg ataaagaatg
     3061 cattgcgtgt tgtttctaag aaatctgtag caatttgttc cataattggt gcatcgtcaa
     3121 caattgcttc tattgctgtt gttacttttg ctaattctct caataaatca atatctgctt
     3181 cacgaccatg ctcttttgca acgatttgag acaagattga taatactgca atttgtgcag
     3241 tatatgcttt tgtagatgca actgcgattt caggacccgc gtgtaataac aatgtgtggt
     3301 ctgcttcacg tgataaagtt gaacctgcaa cattagtgat tgttaatgat ttatgaccta
     3361 atttattagt ttcaactaat acggcgcggc tatctgcagt ttcacctgat tgagaaatat
     3421 aaacgaacaa tggtttttca gataataatg gcatgttgta gacaaactct gatgcaacgt
     3481 gtacttcagt tggtacgcca gcccattttt ctaaaaattc tttacctact aaacctgcat
     3541 ggtagcttgt acctgctgca ataacgtaaa tgcggtctgc ttctttaaca tcattgatga
     3601 tgtcttgatc aattttcaag ttaccttctg catcttgata ttcttgaata atacgacgca
     3661 ttactgctgg ttgttcatga atttctttta acatgtagtg tgcataaaca cctttttcag
     3721 catctgatgc atcaatttca gcaatatatg aatcacgttc tacaacgttt ccatctgcat
     3781 ctttaataat aacttcatct tttttaacaa taacgatttc atggtcatgg atttctttat
     3841 attcgcttgt cacttgtaac attgcaagtg cgtctgatgc gataacattg aaaccttcac
     3901 caacacctaa taataatggt gatttatttt tagcaacata gattgtgtct ttgtcttcag
     3961 catctaataa acctaatgca tatgaaccat gtaataatga cacaactttt gtaaatgctt
     4021 cttcagttga aagtccttga tttgaaaagt attcaactaa ttgaacgata acttctgtat
     4081 ctgtttctga aatgaatgat acaccttgta agtattcacc ttttaactct tcatagtttt
     4141 caataacacc gttatgaact agagtaaaac ggccatttga tgattgatgt ggatgagagt
     4201 tttcatgatt cggtacaccg tgtgttgccc aacgtgtgtg accgattcca acaggtccat
     4261 caaaatcgct actatcagca actttacgta attctgcaat acgacctttt tctttaaata
     4321 cagttgtatt atcatcattt actactgcga tacctgcaga gtcataacct ctgtattcta
     4381 atttttctaa accttttaat aataattctt tggcattatc atagccaata taaccaacaa
     4441 ttccacacat aacgacattt tcctccatat tggaatagta cgcgtaaatt atgatttatt
     4501 gccgataatt tagattgaca atctgctttc ataatataaa taggaacatg ctatcatcgc
     4561 attcatccat aacaaattaa gcatagttat ttttacaact atacaaattg ctcacactgt
     4621 actttccata ttaatatttt ttatattcaa tttctggcga tcttattaac tttgtccatt
     4681 aagtcaccct aatgttttac ttaataagct aacgaatgag ccacatccgg gatagcatcc
     4741 gccgatctat tcgatcacta tcctcctcgt ctacaaatac atatattgca ctctataaag
     4801 gccactcata tattaacctt taatcttcaa atacaaatat ttatttgcac aggcgcttta
     4861 actgtactgc cgaactttcc ccctttccat taatcattat tgtacaacgg tgttgttttg
     4921 ttttgcaaat attttcacaa taaaatttta aaaatcctaa aacaattttt ttgttttact
     4981 ttttcaaaat atctatactg tcacattgat gacactttat ttaattttgt cacatttatt
     5041 ttgacaaagt tgatttttgt ttatattgag taacaagtaa cctctctata cactatatat
     5101 agtcacatat attaaaaaag aggtgtaaac atgtcacaaa ctgaagagaa aaaaggaatt
     5161 ggtcgtcgtg ttcaagcatt tggatcattc ttaagtagta tgatcatgcc aaacattggc
     5221 gctttcatcg cttggggttt tatcgcggcc atttttatag ataatggatg gttacctaac
     5281 aaagatttag ctactttagc aggaccaatg attacttatt taatcccatt attgatcgca
     5341 tttagtggtg gtcgtttaat ttatgattta cgtggtggta tcatcgcagc aacagcaact
     5401 atgggggtca tcgttgcatt acctgataca ccaatgttac ttggtgcaat gattatgggt
     5461 ccacttgttg gttggttaat gaagaagacc gaccaattga ttcaacctag aacgccgcaa
     5521 ggttttgaaa tgttatttaa taacttttct gctggtattt taggatttat catgacgatt
     5581 gcaggattta aaattttagc accacttatg aaatttatta tgcatatttt atcagtggca
     5641 gttgaagcat tagtacatgc acacctactt ccacttgtaa gtattttagt agagcctgca
     5701 aaaattgtat ttttaaacaa tgcgattaac catggtgtat tcacaccgct tggtgcagat
     5761 caagctgcaa aagctggtca atcaatttta tacacaattg aatctaaccc tggaccaggt
     5821 ttaggtatct tacttgctta tatgattttt ggaaaaggca ctgcaaaagc aacgtcatat
     5881 ggtgctggaa ttatccactt cttaggtggt attcatgaaa tttatttccc atatgtatta
     5941 atgcgtcctt tattatttat cgctgttatt ttaggtggaa tgactggtgt tgcaacttat
     6001 caagcaacag gatttggatt taaaagtccc gcgtcaccag gttcatttat agtttactgt
     6061 ttaaatgcac ctagaggtga attcttgcac atgttactcg gtgtcttcct tgctgcactt
     6121 gtatcattcg ttgtagctgc tttaattatg aagttcacta gagaaccaaa gcaggattta
     6181 gaagctgcga cagctcaaat ggaaaatact aaagggaaaa aatcaagcgt tgcttctaag
     6241 ttagtatctt ctgataaaaa tgttaataca gaagaaaatg ctagtggtaa tgttagtgaa
     6301 acatcttcat cagatgatga tcctgaagcg ctattggata attacaacac tgaagatgtt
     6361 gatgcacaca attacaataa tataaatcat gttatttttg cctgcgatgc gggtatgggt
     6421 tctagtgcaa tgggtgcaag catgttacgt aataaattta aaaaggcggg cattaatgat
     6481 attacagtta caaatactgc gattaatcaa ttgccaaaag atgctcaatt agttatcact
     6541 cagaaaaaac taactgatcg tgctattaaa caaacaccaa atgccatcca tatttcagtg
     6601 gataatttcc ttaattcacc aagatatgaa gaacttttaa ataatctaaa aaaagatgat
     6661 caagcataat aattaaataa attaaaaaat ggaggatacc gccatgttat tgagtacacg
     6721 tgaaaaagaa atgatagccc tattgattaa gtaccacggt caatatatca ctatacacga
     6781 cattgctcag caacttgcgg tgtcctctcg tactattcac cgtgaattaa aaggtgttga
     6841 agcatattta acttcatttt cattaacttt agaacgcgca aacaaaaaag ggctacgcat
     6901 tgctggcaca gattctgatt taaacgattt gaagcaatcg attgcacaac atcaaaccat
     6961 tgacttatct gttgaagagc agaaagtaat tattatatac gctttgatac aagccaagga
     7021 gccagttaaa caatatagtt tagcgcaaga aattggcgtt tctgtccaga ctttagcaaa
     7081 gatgttagat gatttagagc ttgatttaaa taagtaccaa ctatctttat ctcgaaagcg
     7141 tggcgaaggc atttacttgg taggtactga atcaaagaaa cgtgaatttt taagtcaatt
     7201 aatggtgaat aacttaaata gtactagcgt ttattcagta attgaaaatc attttgtctt
     7261 tcattcatta aatcaaatcc acaaagactt tgttgactta gagcgcattt ttaatgttga
     7321 aagactatta atggactacc taagtgcctt accctaccaa cttaccgaat caagttattt
     7381 aactttaact gtccatatcg tgctctccat ttcacgtata aaaaatggag agtatgtcgc
     7441 attaaacgat gatatttatg attctgtaca aaacacattt gaacacaaag tagcaagcga
     7501 acttgctgat aaacttggtc aaatatatga cgtcacgttt aatcaggcag aaattgcttt
     7561 cattactatc catttacgtg gagctaaacg aaaaaatctt aatgatacat cattaaataa
     7621 tcgttgtgaa gaaaacaaaa ttaaagcgtt tgttaacaaa gtagaaatga tttccggtat
     7681 gacatttgca gatttggata ctttagtaga tggactgacg ctacacctta atcctgcaat
     7741 caatcgtttg caagctaata tcgagaccta taatccgtta acagacatga ttaagttcaa
     7801 atatccaaga ctatttgaaa atgtaagatt agctttaaat gattgttggc ctgatttgat
     7861 ttttccagag agtgaaattg cttttatagt tttacacttt ggtggctcga ttaaaaacca
     7921 aggtaatcga tttttaaaca tattagtcgt ttgcagcagt ggtatgggaa ctagtcgtct
     7981 attatcaact cgtctagagc aagtttttag tgagattgag cgtattacac aagcatcagt
     8041 cagcgatttg aagtcactag atttaagtca atatgatggc attatttcta ctgtgaattt
     8101 agacatcgac tccccctatt taacggtaaa cccattatta ccagatagtg atatcagtta
     8161 tgtcgcacag tttttaaata caaagtctac gttccaagag acgcatgata aatcatcaaa
     8221 catgattgat aaggatgatg ttcatgttga aacgaaagat gttgatggca acacatcttt
     8281 tgaaaatgaa caaacttcat acttaacttc agttttcgaa aaacatttaa gtgacgaaaa
     8341 atcagaacaa ttattgcatc atatgcgttc gggtttaact ttgcttgatt cagtgaaaat
     8401 agttagtacc gaagttaaac agtggcaaac atatatcgca gattatctat atcaatgcga
     8461 tgtaataaac gatccaacgt cattcgctga actactagag caacgattga ttgacaatcc
     8521 aggatggata ttaagtccat atcctgttgc aataccacac ctaagagaca atatgattaa
     8581 acaccctatg attctaatca cagttttaga agaaccgtta acattgccta gtattcaaaa
     8641 tgacaatcaa acaattaaat atatgatttc catgtttatt tctgacaatg attttatggc
     8701 atcactggta agtgacttgt ccgaattttt aagtttgaaa ttagaatcta ttgatacttt
     8761 tatggaaaat ccacaggaac ttgaaacatt attaagaaac aaatttttag aacgaattaa
     8821 aaaacaattt atttaggagt gtacaaaatg agcgaattat ttagtaatga caatatcttt
     8881 ttaaatgtaa atgttaacag ccaaaatgaa gcaattgaaa aagcaggtaa agccttagtt
     8941 gatagtggtg ctgtaacaga tgcttatatt caagcaatga aagatcgtga gcaagtcgta
     9001 tcaacattta tgggaaatgg cttagcaatt cctcatggca cagatgaagc taaaacaaat
     9061 gtgattcact caggtttaac attattacaa atccctgaag gcgttgactg ggatggcgaa
     9121 gtagttaaag ttgtcgtggg aattgctggt aaagatggcg aacatttaga cttgttatct
     9181 aaaattgcaa ttacatttag cgaagaagaa aatgtggatc gtatcgttca agcaaaatct
     9241 gcagaagaaa ttaaacaagt attcgaggag gcagatgcat aatgaaagca gttcactttg
     9301 gtgctggtaa cataggtcgt ggtttcattg gttatattct tgcagacaac aatgttaaag
     9361 taacatttgc agacgtcaat gaagaaatca ttaatgcttt agctcatgat catcaatacg
     9421 atgttatttt agctgatgag tctaaaacaa cgacgcgcgt gaataatgtt gatgcaatta
     9481 attcaatgca accttctgaa gcgttgaaac aagcaattct agaagctgat attattacaa
     9541 cagctgttgg tgttaacata ctacctatta ttgctaaatc ttttgcgcct ttcttaaaag
     9601 aaaaaacaaa ccatgttaat attgttgctt gtgagaatgc tattatggca actgatacat
     9661 tgaaaaaagc agtacttgat attactggcc ctcttggtaa caatattcat tttgctaact
     9721 cagcagttga tagaattgta ccattacaaa agaatgaaaa tatattagac gttatggttg
     9781 agccatttta cgaatgggtt gttgaaaaag atgcatggta tggtccagaa ctaaaccata
     9841 ttaaatatgt tgatgattta acaccatata ttgagcgtaa attattaact gtgaatacag
     9901 gacatgcata tttagcgtat gctggtaaat ttgcaggtaa agctacagtt ttagatgcag
     9961 ttgaagatag ttcaattgaa gctggcttac gccgtgtttt agctgaaact agtcaatata
    10021 ttactaatga atttgatttt actgaagcgg aacaagctgg ttatgttgaa aaaataatag
    10081 atcgtttcaa caattcttat ttatctgatg aagtaacacg tgtcggacga ggtacattac
    10141 gtaaaattgg ccctaaagat agaattataa aaccattaac atatctttat aataaagatt
    10201 tagaacgcac tggtttatta aatacagctg cattgttatt gaagtatgat gatacagcag
    10261 accaagaaac tgttgagaaa aataattaca ttaaagaaca cggtttaaaa gcgtttttaa
    10321 gtgaatatgc taaagttgac gatggcttag ccgatgaaat aattgaagcg tacaattcac
    10381 tttcataatt tattgagctt tgtttgaaac aagaagtttc caacgttatt cgttaacaat
    10441 cagtaataat gtagtagttc ccttgaatta acaatattaa atttctgaac ataaaaaata
    10501 ctcccttcaa catagacact taacttgtgt tatgtatgaa aggagtattt ttgcgttaat
    10561 aatttgtttt attttcgagc cacagccacc tattcaatgg ctattggtca ttactaaaac
    10621 aaattcatat taactgttag acttggttac ttagtaagga atatttccct atgaaataac
    10681 tagatgttca cattcttgaa taaattttat tcttcagttt gttggtcttt cttagtgaat
    10741 cttctaatta agaatgccat acctgcacct agagctaatt cagcatatgg taaatcgtca
    10801 ttatgtgaca taccagtatc tggtaaagtt ttagcttgtt gtttagcttt attaactttt
    10861 ccttgttgag ctgattttgt cttagcttgg tggtcgtcag tgttagttac attaagcata
    10921 tcttgattag cactattgct tccatttgaa actgtagctg gagatgcatt ggcaccgtcg
    10981 ttttgcgtag ctttattgtt tgcagctgaa ccaactgatt tttgcgtatc attagtatct
    11041 gctgttgccg tatcatcttt ttggctaaca ttagttgaag tcattttttc ttttgcttca
    11101 gaagatgcag atgttgatgg tttattcgaa acttcagtat cagctttgct tggcgattta
    11161 tctgcttcgt tagatgcaac gttagtttca gacttaagtt gtcctgcatc agtttgattt
    11221 gtcgtacttt cttctttatc ttttgatgta ttagaaggta catttggttc tgttatgtct
    11281 gctgaaggca atgtttcagt tgttgattca accatacttt gatttgttga atcactacca
    11341 tctttttctg ccttagcttt attttcagat tttggttgtg caaccttgtc attagttgat
    11401 tgagattcag cactattatt tacttcagca ttttgttttg aatcatttac agatgcatta
    11461 tctttgctat cagtagatga tgctgcttct gtgctcgcag ttgttggagc cgttgctgtt
    11521 gatcctgttg gtgcattctc gtttgttgct gtagttgtac tattgttatt tgttgtgctt
    11581 tctgctggcg ttgcattatc agtttctgtt acaggtttat cagttgtgcc gttattagtt
    11641 gattctactt ctggtttact agttacatcg ttatccattg tcggactgtt tgttgatgca
    11701 tctacactag aattgttatt agcttgcggt ttatcatttg catcatcagt tgctgatgtt
    11761 gctgttgttt cacctgttgc cgcatcacta ttatttggtg ttgtcggaga agcgtctgct
    11821 ttgccattag ctgtcgtctc agatacgtta ggttgtccag tattttctgg tgttgcatta
    11881 gcatttgaat ttgctgttgc atcattatta tctataccat tattagtatc attagcatct
    11941 ggatcattct gaggcacaat cgcttcaatt gcaggtatcg ttacattttg taattcagca
    12001 acttctgcat ttgtttgtgt tttatctaat ttatcagcaa atctgtcaaa atatctacct
    12061 aaatccgtac gtgcaatttc tttcgccgat gcatctgcat ctgcattttt aattatttct
    12121 atttgcttgt taaccacttc tctgattgct tccaaagcat ttttcttaac ttcaggatta
    12181 atacgttgtg ctttaagttg ttcaagcgca ctatttttga cagtagcgat ttctgcattt
    12241 gtagtttgat cagaaatatc ttcagttgct tttgataaaa tgtcttctaa agcattcgta
    12301 aacgcttctt tttcttcagt tgtagcatca gcgttgacat ttacacctgc ttcaatctgg
    12361 tctagtgcag tttctaattc ttcgatagct ttttgttttt ctgttgagtc gatttgaatg
    12421 ttatcaaatg cttcaagtcc ttgagctttc gctttttcaa cttcagcagt tgttgttgca
    12481 tcagtaatac cttgtttagc ttgatctgta atttgtttaa tcattgctaa tgcttcagtt
    12541 ttttcttcag cagttaactg gtcactttga tcaatagatt ctttcgtatc ttctgcttta
    12601 acttcaatag cttggttcgc tttaggttta acagtagcat ctacttgaat agcatcaatt
    12661 gctgctttac cttgtgttaa tgcatcatca acgtcaccat tatccacacc attattaatg
    12721 ctttctaatg cagtttgaac attttggtca acttgcttaa ttgcttgttg cttttcatct
    12781 tgtgttgcat tagtgtcagc tgaaatatta tttttcttct gatctgcata agcatataaa
    12841 tctgttgtag ctgattcttt tttacctgtt ggaattgtgt aatcgttaat attatctaag
    12901 tcattatgaa tttgaacttc aatgtcatct ttagaagtag cttgattaac attttgatcc
    12961 gcagtttgtt ttaattcagc aagtttttgt ttcgcttcag caatttcact tgaagtcgat
    13021 gcgttagagt tatccgcttc gtttacttta gcattatatg catcttcaat tttagctaaa
    13081 gcatcttttt tgtactcact aaatgtttta actgcattaa ttttagcttt tccttcttta
    13141 actgcattat caacatattc atttgttgat gattgatcga catttgtttt cgcttgattt
    13201 aattcagtat caacttcttg ttttgcatca ttaatttctt gttgtgatgc atttggtgtt
    13261 tgttctattt gtgttttctt atcagctgca gcttgatcta attctttttt acctgctggt
    13321 ttcttaacag gatttgcatg aagttgttga actttttgta ctgctgtatc tttagcagta
    13381 gttacatcac ctgtagtagt tgctgcatta atattattta aaccttcttc atatgctgct
    13441 ctaactggtc caatatcgtt acctttttct tcattagtag tctcattatt attaagtatt
    13501 tcagttattt tattttgcat ttcagttagc aattccgctc ttgcattcgt tttaacatct
    13561 gttgatgctt gaattgggtc aattgcttga attgcattat cttttgcagt gtttacatca
    13621 tcgattgact gtgcattttc aatattttga ttaccttgtg ttaattgtgc gtctacttgt
    13681 tgatttgctt gttctttttc ttcagtagtc gcatctgcag tttgtgcaat aagcgctttt
    13741 tgttcgtttg cttttgttgc taattcatct ttcgcaacat ctttaattgt tgtatctgca
    13801 gtaatacctt gaatatcagc aactgcttga tctttaattt gcgtaacatc attagttgtt
    13861 tgtgcattta agatatcttg atacgctttt tctttagctt ttaaaactaa atcttttgct
    13921 gcatttttct cttcagttgt agcaccagtt gtattatcaa ttgcttgatt ttgagttgtc
    13981 acagcttgat caacatcatt tttagcattt gatttaaccg ctgttgctgg ttgcgtgctt
    14041 tgaattgaat tctttccagc gtctttcgcc tgatctacac cattatcatc agttgcagct
    14101 gtaatattat ttttcgcgtc tgtaactgct gttgctaatt gttgaatagc tgcttctttt
    14161 tcttctgttg tagcgttctg atcattattg ataacatttg tttgcgttgc ttgtaattga
    14221 tcaatttcat ctttagccgc ttgtttcttc acaacttttg gtgttaccgc attaatcgct
    14281 gcttctgcat ttgctttagc ttcatcaact tgtgcgttag tagttgctgc tgaaatggct
    14341 tgatttgctt taccattttc agtatttgct tcagcatcag ctgcttgttt ttcttcatct
    14401 gttgcatctg gcgtagcttg aatctcttgc aatttgttat ttaaaattgc tgtgatttca
    14461 ttacgtgcag ttgctttttt attaactgtt ggtgttactt gatcaatact attttcacct
    14521 gtcgttttcg cttggtctac atcattttga ctattagcag cttcaatgtt gctatttgct
    14581 tgtgtcacag cattatctac gtccgcatta gccgctgcaa tttcttcagc agtaatgtct
    14641 tgcgtttgag cgattgctgt tttacgttca ttcgctttcg tagcaattgc ttctttcgca
    14701 ttatctttag ttgttgttgc tggctgaatc gcttcaattt tagcaattgc tgctttttta
    14761 gccgcttcaa cttccgcatt tgtatgtgct gcatctattg cggcatcagc tgttgttttt
    14821 tcagtttgaa cttgttgttt agcagctgct ttttcttcag ttgttgagcc gttatttcca
    14881 tcaattgctg tttcttgagc ttgtacttta tctgcaattg cttgttttgc tgctggttta
    14941 acatttgcat caggtgtaat ggctgcgatt gtagcttcat ttgtagtttt tgcattatcc
    15001 acatcattgt ttgctgcagc attatctata tcagcgtttg cagtaactac tgcttgatcc
    15061 actttgtctt tcgctgcttg ttgttcttca gtagtcgaat cattcattgc ttcaattgct
    15121 gttttacgtt cacttgcttt ttgagcgatt tccgcttttg catccgattt cttagttgtg
    15181 gcagcttgaa cttgattaat tgcagcaata ctattgtctt tagctgttgt tacatcactg
    15241 tttgtatttg cagcatcaag atttgttctt gcttcttgct ttttagtatc taattctgta
    15301 tatgcagctt gtttttcttc tgtagttgaa gcattgctat tttgaatttc ttgtttacgt
    15361 gtattatatg cgttttctac ttccgtatca gctgcaggtt taacttttgc ttgtgtttga
    15421 attgcattga ttttatctaa tacttttgat tttgtatcag caacttcctg ttttgattta
    15481 acaacttgga tgtcatgtaa accttgcttt tgagctgcat ctactgctgc atcagcttct
    15541 gctacttctt catttgttgc atttgaaact gtagcatttt gagcttttct agctgttgca
    15601 gcttgtttaa cttcattata ggcatccatt tttgtttgcg tagagtcagt aatattttca
    15661 atttttgaga tttcttcgtt tttaactctt tctaaatctt gtgcagtcgt tgtcgcttca
    15721 attgctttaa caccagaaac tttagctgca ttaatacgtt caatagcttc cgctacttct
    15781 tcatttgttg tatcaggatt taaaggtgct tgatcaattt gtgcttgaac aacttcgtca
    15841 aattcttcaa gagctgctgc tttagctgtt gtagcaggtg tagtttgatt aatatcattg
    15901 attgcttttg ttttaatggc ttctacttgt gcatttgttg ttgctgcatc aatatcttta
    15961 attgcctttg tttcaatttt accaatttta tcatttgcta catctttttc atcttgtaat
    16021 gatgcatttg actttttaat ttgttgttta cgagttgtaa ctgcttggat aatatcttgt
    16081 ttcgcttgag gtttaactgt tggtgtaatc acatcttgtt ctaacactgc gataccatta
    16141 tctttttcag ttgtgacacc ttgtgctgta gtttgtttat taatttctgc gattgcatta
    16201 tttttatcat tgttaatttt agttacaagt gctgcaactt catcttgcgt aactttttta
    16261 ctttgttgtg ctgctgtaac cttttcttga gctttagtat ctaattcagc aattgctgct
    16321 tgattatcaa ttaatgcagc ttgtaattta gttactaaac catcaatatc cgcttgagat
    16381 gcacgatttg cagttttaac atgattcgca tcttcattta aaatagtatc tgcttgttgt
    16441 ttaagtttat tgtattctgc aattgatgct gttgtgtaat gactattatc aacttgtgag
    16501 tttacttgtt gttgcaacgc atctttgttc atttcaaccg ctactgaaaa tggatctgca
    16561 gttagtgtaa cttctggttg cgcattatta attacaacat ctgaagcagt acgatatgtt
    16621 aatttttcat taaaatcaat atttttaggt gtatcgatat tcgcaacatt aactttatat
    16681 gataatttta aagatttatc tgggaataaa acttctttag tgtgtgtacc acgtgccgtt
    16741 gtcacacctt ggcttgtaaa tgtaacttta tttgcatttt gatcataatt aacagtcata
    16801 tttttcaata ctgtactgtc ttcattacca ttagggaatg ttgtagttaa tgaattatta
    16861 acgtaagtta caccttgtgg taatgttact tcatatttaa attgatctgc ttttaatgaa
    16921 gcaccaaaat taccattgtt accgatttca gtattaatag tatattcttt attgttagtt
    16981 gctttcggtg ctaaatctct atttttgaca tataaatgtg atcctgagaa tagacctaca
    17041 ttatcaatga atgaatagta tttataacca tctttatttg ttgtaattct tgaagcatca
    17101 gcaactgctg tattgtcagg aataaattgt aaatctaaac gatcagcatc agttggtact
    17161 ttaaataaat gtgcagtacc agtcttagca acatttacag ttgctaattc agtatttttc
    17221 ttagcatctt taataactaa atttgttgca ccttgcttac tattcgtttg catagtagta
    17281 aagttaataa ttaattctga atctggtttt acatttacag tttttgaaat accgttaaag
    17341 ttaccatgat ctgtagaatc atttgcattc acacgaccta atgcagccac gtttccttta
    17401 gcttgatagt tttgagggtt attcttatca aacatatcgc ttcgtcttaa ttctgagtta
    17461 acgaaaccaa tcttaccgtt gttaattaat gaataaccat ttactttatc tgtaacagtt
    17521 acagttggat cctgtctatt ctcatctgtt gatatggcag gatcatcaaa tgttaatgtc
    17581 gtattaatac tgccttcacc agtattgcta gcatttggat cttgagtttg tgcgtttgct
    17641 gctacaggtg ctgctggttg cgctgctgct ggagcattcg ctggctgtgt ttgatttgcc
    17701 ggtgttgcat tattattagg tgttgcttgg ttatttcctt gacctgcttg gtttgccggt
    17761 gttgcttgat ttccaggttg tgcttgtgca gcgttattcg gatcagcttg attaccttgt
    17821 ccagctggtt gtgtatttgg ttgtgctgct cctcctgctg gattagcctg tccaccttgg
    17881 tttgctggtt gtactgctgg ttgtccttgg ttggcaggtg cagctggctg tgctgtagga
    17941 ttagcttgag caccagcatt tgcgttaggc tgtgtattgg catcagctgg ttgtgctggt
    18001 tgattttgtg caggctgatt ttgctctgct gcagacgctg ttgtcgggtt agtagatata
    18061 aaagtaacag tggcaattaa agctgaaaaa ataccgacat taaattttct gatactaaat
    18121 ttttgttgtc tgaataaatt cattaagtca tcctcctggt tgattattct cgctgttaaa
    18181 tgatttcact taatcaactg ttaagataag tagtagcatc tgcgttaaaa acacaaaaca
    18241 actctatcta attaaaatta attttatcat cattatatat tgagtaccag tgtattttat
    18301 attacatatt gattactttg tttttatttt gtttatatca ttttacgttt gtactataaa
    18361 ttatttctac aaacacaaaa aaccgatgca tacgcatcgg ctcatttgta atacagtatt
    18421 tatttatcta atcccatttt atcttgaacc acatcagcta tttgttgtgc aaatctttca
    18481 gcatcttcat cagttgctgc ttcaaccatg acacgaacta atggttctgt tccagaaggt
    18541 cttactaaaa ttcgaccttc tccattcatt tctacttcta ctttagtcat aacttcttta
    18601 acgtcaacat tttcttcaac acgatattta tctgttacgc gtacgttaat taatgattgt
    18661 ggatattttt tcatttgtcc agctaattca cttagtgatt taccagtcat ttttattaca
    18721 gaagctaatt gaataccagt taataaacca tcaccagttg tattgtaatc catcataacg
    18781 atatgtccag attgttctcc acctaagtta taattaccgc gacgcatttc ttctactaca
    18841 tatctgtcgc caactttagt tttattagat ttaattcctt cttgttcaag cgctttgtaa
    18901 aaacctaaat tactcataac agtagaaaca atcatgtcat tattcaattc ttgattttta
    18961 tgcatttctt gaccaataat aaacataatt tggtcaccgt caacgatttg accattctca
    19021 tctactgcta tgattctgtc tccatcgccg tcaaatgcta acccaaaatc actttcagtt
    19081 tcaactactt tttcagctaa tttttcagga tgtgtagagc cacatttctc attgatatta
    19141 tatccatcag gactacatcc aattgtttca gtatctgctt ctaagtcacc aaataagaat
    19201 ggcgctagtg atgatgttga accatttgca ccatctaaag caattttcaa accttcaaag
    19261 ttaacatcta ctgttgattt taaatagctc aaatattttt gtgccccttc aaagtaatct
    19321 gaataatgta caatatcatt gccaactggt cttggtaatt ctgggttttc ttgatccaat
    19381 aatgcttcaa tttcattttc ttgttcatct gatagtttaa aaccatctga tccaaagaat
    19441 ttaataccat tatctgcaac tggattatga gaggctgaaa tcattacacc taactctgca
    19501 cccatatcgc gtgttaaata tgcaacacct ggtgttgaaa taatacctaa tcgcatcact
    19561 tctgcaccaa ttgaaatcaa accagctatt aatgctgatt ctaacatttc acctgaaact
    19621 ctagtatcgc gacctacaag tacacgtggg tgtttttcac ctttattatg tgctagaaca
    19681 tagccaccgt atcttcctaa tttaaatgcc aattcaggtg ttagttcttg gtttgcgaca
    19741 cctcttactc cgtctgtacc aaaatatttt cccattgttt actctccttt aatttagcta
    19801 tttattttac atttatataa gccttcgttt cacttggttg tgctttagtg acatgttctg
    19861 gtaaattgat ttttacagtc ttttcagttg attctgaaat accatctaaa tctacttctg
    19921 catcaacttc gcttatattt tgtaagtcat ctcgactacc gaagatttca atttctttat
    19981 cttctaaatc aatcgaactt aactctttat catctgctaa actacctttc tgtttaacat
    20041 ttactttaac ctttttgcta aaaggctcta ctttaacttg taaattcact tcattaggtt
    20101 gaatcgatac atttaattta ttcagttttt tatcaaaagc cgttacttct gcgacatctt
    20161 ttgtgtcacc attaatcttt ttattagttt taaaagtggc ttttaaataa gcgatatcat
    20221 tcaattgttc ttctccacct gttactttaa ctgtttgtgg tgaaacttct tgctttgtaa
    20281 ttttataaag tggatcaata tcactctgac ttacatcagg ttgaactgtc atctttttag
    20341 ttactttgtt ttcaagcgta atatttgcta atttaggttt tacagaataa tgaatgtcat
    20401 cagctaaccc tttaacttga tacttctctt gatattttcc agctttagca tttgataaat
    20461 caatcactac tcttaaatct tctggatttt caatttttat tatctttgat tgtggtcctg
    20521 aaatagtcac attaactgtt tcaggcgctt ttgttaaatg caaatcttta gtgttataaa
    20581 gaatttcaac gggtacatct tgaatcgttt tactagactt ttgaccaaga ttaccagtgt
    20641 taaagatatt tccaaaaaca ttgttaacag ataaaaagaa aaacaatgcc aaaagaaagg
    20701 caataaatct caagccccat ttactttcta gcatattatt tcacaccttt cttttgaaag
    20761 cgtgtgccaa accaatgttc agcaagcaac tcttcaaaaa tttcgtttga aatgtctcgt
    20821 cgtaattttc catcaaatgt taccgaaata tcaccagttt cttcagatac aataacggta
    20881 aatgcatcag atacttctga aataccaacc gcagctctat gtcttgtacc caaactttta
    20941 gatatcttag gactatcaga caatggcaaa taacttgctg ctgctgcaat cttcgtgcct
    21001 tgaataatca ttgcaccatc atgtaaaggt gtgttaggta taaagacatt aattaaaagt
    21061 tcttgcgaaa tatttgaatc cattgcaata cctgtttcaa tataatcttg aagacctgtt
    21121 tctttttcaa agacaattaa tgcacctata cgtcttttag ccatatattg cacagccttt
    21181 gaaaccgatt gaatcaattt ctcttcatct ttactatacg tattagaagt atagcgtttt
    21241 aaaaagctac ctctaccaag ttgttctaac gcacgtctaa tttctggttg gaatattact
    21301 attaaagcta atacccccca ttgaataacg atatcgaata atttagatgt tgcagtcaag
    21361 ttcaatatca tacttatctg ctgaccaata acaattacta atatcccttt aagtaattgt
    21421 atcgctttag ttcccttaaa gaccgtgatg agaagataaa gtacatacca aactatcagt
    21481 aaatcaagga tactcgttac aatttttaac gtactgaggt tttgaaaaaa gttggaaaaa
    21541 tccataacat ctcctccggg taatattttt ccataatacc cattatacca atcatttaca
    21601 tcactgtcat acgaacatat atttaaatag aaaaaattta ttttaaagat tataactact
    21661 cttaatcatt ttagtgaatt aaaaaaagta gtgcaaaaag caaaatatac tttatacact
    21721 acaaatcatt tatttataat aaagtttcac caaaaaatgt tccaactaat gaaaccgctt
    21781 gttcagcagt atgattatta ctgtcaatca atggatttac ttcaactaaa tccattgagg
    21841 aaattaaatg tgattgatgc agtaattcca atgcaaaatg gctttctcta taactaagac
    21901 cacccaaaac tctagtacca gtgcctggcg tttcaagcgg atctaaagca tcaacatcta
    21961 aagataaatg aacgccatca acattgcgtg acttcaaata ttctattgta ttttcaatta
    22021 cttcctttat ccccaattta tcaatatctg acatagtaaa tgttttaata ttatgatctt
    22081 tgataaattg tctttcacct ttatctaaat ctctcatacc aattagtacg atgttttctg
    22141 gcttgattac attactattt aattctaaaa gttctttggg accttcgcct gtcaaaatcc
    22201 ttagaggcat accatgaata tttccacttg gtgactcttc aggtatattt aaatcaccat
    22261 gtgcatcata ccaaataaca cctaaattat tataatgttt acttattgct gatactgaac
    22321 ctaccgcaat agaatgatct ccaccaagaa ctagaggaaa tctgttattt tcaattgatg
    22381 ctgaaacctc tttatttaat ttttgattaa catctataat ttcatcataa tttcttaatc
    22441 ctttttgttc actatgaaat ttttcaatgt tcacagcagg taccttaata tcccccttgt
    22501 catatacatc aaggtctaat tgctttaatc ttgaaattaa tccagcatat ctaattgctg
    22561 ttggtcctaa atcaacacct aattttcttt gtccaaatgt tgatggtgca cctataatat
    22621 caattgcttt tgtctttgtc ataagcgtcc ccctttgctc tatgtaatta aagaataatg
    22681 tatgcgctta ccattatcaa gcaatagcta cacatataat ctgtttatct tattacttca
    22741 taaaaaaagg ttcttcatct tttatggtgg gaaggtaaaa cttcctgctt tttttaatac
    22801 acaaaaagcg caattgcctc tataatttaa agtgaccaaa cccaaactaa aggagacaag
    22861 tgcgcctatg tgtaatgata ccttagaatt actaagaata aaagatgaaa atataaaata
    22921 tataaaccaa gaaattgacg tcattatcaa aggaaaaaaa gcaacagtgg ttaatgctgt
    22981 actaacgtat aagccttcgg cctgttattg ttgtggagtt aaaaatgaag gacaaattca
    23041 taaacatggt aagcgtgttt ctcgtattac tttacttaaa actcaagggt ataacacata
    23101 cctcaactta gctaaacaac gttttaaatg cttagaatgc aatggcactt ttactgctaa
    23161 aacgtcaatt gttgatgagt cgtgttttat ctcaagatgt gttactcaaa aagttataga
    23221 agaagctact aaagttaaaa cagagattga tactgcagaa gataactgta tctctccatc
    23281 tactgtaagt cgtattagaa ctaaagcggc taattcatta cgaattaaac cctttaattg
    23341 tttgccagaa cacatcgcta tggatgaatt taaaagcgtt aaaaatgtaa ctggatcaat
    23401 gagtttcatt tttatagata atgatactca tgatgttata gatattttag aaaatagaac
    23461 tacaagattc ttgcgtgcct atttcgagcg attcgattta aaaaatcgac aacaagttaa
    23521 gacggttact attgacatgt atgaacccta tgtccgatta tttcgcgacc tatttcctaa
    23581 tgcagctatt atttttgaca gattccatat cgttcaacat ttaaatagag aacttaataa
    23641 gtatcgtgta caagttatga atgaataccg taataaaaaa ggacctgatt atacaatttt
    23701 taagaataac tggaaagtcc tattgatgga tactagtaaa accatattta gtaaatacag
    23761 atggaataaa tcttttaagg cttataaacg ctcatctgac attgtagaat tcatgctttc
    23821 aaaagacgat atactacgat actcctacga acttgtccaa ggattacgaa aagacctaag
    23881 gttatgtaat tggcctaaat ttattaatcg tttaaattca gttagtaaaa agtctgtgag
    23941 taagggtgta tggaaagtgg ttaaatatta tagaaaacat caaaggatgt taagaaatac
    24001 aatttattac ccagcattta ataatggtgc tatagaagga attaataata agataaaatt
    24061 aatcaagtga atttcttttg gttacagaaa tttcaacaac tttaaagcac gtataatgat
    24121 gattttcagc ttgtacaaag gagaaaaaaa gaagacaacc aagcccaata atggactggc
    24181 cgcctaataa taaaagctct aaaagttgta ttttaaaaat agttctttaa attatatacc
    24241 caccacattt ggtggagaac ctaaaaaaaa gcacttccca aaaatggaaa gtgcaagtag
    24301 tgagccatag aggattcgaa cctctgaccc tctgattaaa agtcagatgc tctaccaact
    24361 gagctaatgg ctctaatggc tgggctagct ggattcgaac caacgagtga cggagtcaaa
    24421 gtccgttgcc ttaccgcttg gctatagccc aatatataga tggtggaggg gggcagattc
    24481 gaactgccga acccgaagga gcggatttac agtccgccgc gtttagccac ttcgctaccc
    24541 ctccagctta ttcatataat ttaataatca aaatggtgga gaatgacggg ttcgaaccgc
    24601 cgaccctctg cttgtaaggc agatgctctc ccagctgagc taattctcca aaataatgac
    24661 tcctacggga ctcgaacccg tgttaccgcc gtgaaagggc ggtgtcttaa ccgcttgacc
    24721 aaggagccat ggctccacag gtaggactcg aacctacgac cgatcggtta acagccgata
    24781 gctctaccac tgagctactg tggattaata tt
//
LOCUS       DAXWFK010000019        24584 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.019, whole genome shotgun sequence.
ACCESSION   DAXWFK010000019 DAXWFK010000000
VERSION     DAXWFK010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 24584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24584
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.019"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..459)
                     /gene="bstA"
                     /locus_tag="ACGEXS_000627"
     CDS             complement(<1..459)
                     /gene="bstA"
                     /locus_tag="ACGEXS_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol transferase BstA"
                     /protein_id="HFF8011667.1"
                     /translation="MTTKTVFDVIDMGLGYLVNVYDAWKVEKVLDDYHKPFSNTIHWQ
                     FGHVLTIFESALAVAGKENIDLNIYRPLFGNGSSPDEWKDEVPSIERILEGLQTLPER
                     ARNLTEDDLAIELKQPIVGCNNLEELLVLNAIHIPLHAGKIEEMSRILKNL"
     gene            626..964
                     /locus_tag="ACGEXS_000628"
     CDS             626..964
                     /locus_tag="ACGEXS_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFF8011668.1"
                     /translation="MSVSDQIMKGLLDGAILGLIAQGETYGYEIMTKLKNQEFPEISE
                     GSIYPVLMRLNKKGYVDTTIKKSSNGGPRRKYYTITDSGLKELKVFKSKWQVLNKGMI
                     NLFGGENDVK"
     gene            954..1721
                     /locus_tag="ACGEXS_000629"
     CDS             954..1721
                     /locus_tag="ACGEXS_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011669.1"
                     /translation="MLSKQSQDFLEKLRVELLFRGKTEAEVEELLEELEDHLTMAEKN
                     NEDTSSIINTPIKSMADQLSPEISLTTGLYKYITLYFAFILAVIIIPRFLDLGTFDVT
                     IAFLLYIASIVILGVVIGMLILRNTLVRFGDQKITYVINVTYGIIVFLWMILGGLIIK
                     KYPIYSFFELSDKQSFIVGLVLLIALIGTCILIKQKIYALIVLVISLPSLIARIAAIF
                     SKEPDTFNLVSVSILIILNIAFIIYTFVNFRKLKKED"
     gene            complement(1840..2478)
                     /gene="pcp"
                     /locus_tag="ACGEXS_000630"
     CDS             complement(1840..2478)
                     /gene="pcp"
                     /locus_tag="ACGEXS_000630"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFF8011670.1"
                     /translation="MHILVTGFAPFDNQNINPSWEAVTQLEDIIGTHTIDKLKLPTSF
                     KKVDNIINKTLASNHYDVVLAIGQAGGRNAITPERVAINIDDARIPDNDDFQPIDQAI
                     HLDGAPAYFSNLPVKAMTQSIINQGLPGALSNSAGTFVCNHTLYHLGYLQDKHYPHLR
                     FGFIHVPYIPEQVIGKPDTPSMPLEKIVAGLTAAIEAISNDEDLHLALGTTE"
     gene            complement(2630..3586)
                     /locus_tag="ACGEXS_000631"
     CDS             complement(2630..3586)
                     /locus_tag="ACGEXS_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFF8011671.1"
                     /translation="MNYQVLLYYKYMTIDDPEQFAQDHLAFCKAHHLKGRILVSTEGI
                     NGTLSGTKEETEQYMAHMHADERFKDMVFKIDEAEGHAFKKMHVRPRKEIVALDLEDD
                     IDPRHTTGQYLSPVEFRKALEDDDTVIIDARNDYEFDLGHFRGAIRPNITRFRDLPDW
                     IKENKALFADKKVVTYCTGGIRCEKFSGWLLKEGFEDVAQLHGGIATYGKDPETKGEY
                     WDGKMYVFDDRISVDINQVEKTIIGKDWFDGKPCERYINCANPECNKQILVSEENETK
                     YLGACSYECAKHERNRYVQANNISDNEWQQRLTNFDDLHQHA"
     gene            3830..4801
                     /locus_tag="ACGEXS_000632"
     CDS             3830..4801
                     /locus_tag="ACGEXS_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HFF8011672.1"
                     /translation="MSQQDLPTLFYSGKSNSAVPIISESELQTITAEPWLEISKKGLQ
                     LEGLNFDRQGQLFLLDVFEGNIFKINPETKEIKRPFVSHKANPAAIKIHKDGRLFVCY
                     LGDFKSTGGIFAATENGDNLQDIIEDLSTAYCIDDMVFDSKGGFYFTDFRGYSTNPLG
                     GVYYVSPDFRTVTPIIQNISVANGIALSTDEKVLWVTETTANRLHRIALEDDGVTIQP
                     FGATIPYYFTGHEGPDSCCIDSDDNLYVAMYGQGRVLVFNKRGYPIGQILIPGRDEGH
                     MLRSTHPQFIPGTNQLIICSNDIEMGGGSMLYTVNGFAKGHQSFQFQ"
     gene            complement(4950..5225)
                     /locus_tag="ACGEXS_000633"
                     /pseudo
     CDS             complement(4950..5225)
                     /locus_tag="ACGEXS_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
     gene            5279..5563
                     /locus_tag="ACGEXS_000634"
     CDS             5279..5563
                     /locus_tag="ACGEXS_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011673.1"
                     /translation="MTNFALSFCNSNKLDYRSFVILRYHGVSKRHICKMYKIAYFCIL
                     DVCKKAKNNDYQFTYKDYVFLKKYGVSNAFICKMYHIDKLDLEFFEVMNR"
     gene            5720..6022
                     /locus_tag="ACGEXS_000635"
     CDS             5720..6022
                     /locus_tag="ACGEXS_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFF8011674.1"
                     /translation="MIEPVIDIDATMGKEFRYLSHVEKISRFDEVYYLYTIVCVDIGE
                     QVKIRLSNEKHFNALERVDFDNLTIKPRVYQDMNTGYCNLYTSFSADDIYSLETKA"
     gene            6065..6400
                     /locus_tag="ACGEXS_000636"
     CDS             6065..6400
                     /locus_tag="ACGEXS_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF961 family protein"
                     /protein_id="HFF8011675.1"
                     /translation="MAWSPKFDLKETFGDLYFMGVDEKFKYEDGEKTDQKLYAYKLAS
                     TGQGEQVTVKVPKEVKLDIMSVCELVGVEAKQYVQSSGDFHSIQPSIKAEDIRQIGTI
                     SVKKGAQQS"
     gene            6404..6706
                     /locus_tag="ACGEXS_000637"
     CDS             6404..6706
                     /locus_tag="ACGEXS_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011676.1"
                     /translation="MEFYTADNLAPYARTLKLSEGMLSYIASRINTGKALSLMLIAKE
                     IQEKFNGDYVKSRLPSGRPRIYTDVCLLCFSLKEAGHGRLLQIDLKDCIYIGDVDS"
     gene            6886..7962
                     /locus_tag="ACGEXS_000638"
     CDS             6886..7962
                     /locus_tag="ACGEXS_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFF8011677.1"
                     /translation="MENHASTPLTNRGVAKPEKCGLEAVVDWVQVTFQVPSIFTIMED
                     VLKLPRAVFKHRNSGLYYYNRGYTFGNIQIFYSDKDEGMGFHLQLSGSGCREFEHYLI
                     NRNETWQDFFKRCAIKKARFTRIDIAIDDTKTYLKIPRLIKKAERGECISKFRSASSI
                     SGFKLSDGQSKGSTFYIGSKKSDMYCRFYEKNYEMAYKLKTPVEDFGLWNRYEIQMRR
                     SNAENCAMILTETSSISDIVKGVLNNSMRFVTEPKDVSDSRKSRWPVYQEWSRFIKGA
                     DKISLSMKPSLKSIEDNIDWLCKQVATTLDTVLTAEAMAQSEGLLEDTDFLNKILAHS
                     SFNDEHTDRINHYLENLKLKKELT"
     gene            8048..9109
                     /locus_tag="ACGEXS_000639"
     CDS             8048..9109
                     /locus_tag="ACGEXS_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFF8011678.1"
                     /translation="MNIINKLILSRFSKKGKRFDVPEEERKSLPKYRNHRKKIVIIFY
                     TILVLLIILLIASFIRASKANNESKEAVNKTHEIQKKYEDNAETVQYNPNLKLYADKY
                     IDAYMTIPKDGKAKEDRQKVLASFYPSDYKTPTENTENVERKLNEKEFFNIKRKDKQT
                     IIQYIVSYDLKITEKKEVKVKKKKDKKDKDKKDEYETKTEEKERTETKKALLNIPVRS
                     ENNKYVVIEYPHFSTIPSNQLKKATLVKDNLENEKREDNPKLKSFVEEFFDKYAKSKS
                     DDMAYLMDSPEGLEDTREASKISDLRIYPKGDDYVVKAEVLMKDKGSPLENLEHYTLD
                     ITKKDGKYYVKKLTTTIGG"
     gene            9114..9374
                     /locus_tag="ACGEXS_000640"
     CDS             9114..9374
                     /locus_tag="ACGEXS_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFF8011679.1"
                     /translation="MEHLFNLMLVLGDAPTINPLGQWINKEVGTAIGIIVLVIGCVKW
                     ATGKYGHMLALFVIGGLMFLISKGPETVFNSVSGIWKLIFGS"
     gene            9380..9769
                     /locus_tag="ACGEXS_000641"
     CDS             9380..9769
                     /locus_tag="ACGEXS_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFF8011680.1"
                     /translation="MDKKAYNLKRTFEQPIVMYEFTGSYRWSKGIRLDWVGTLFVIEL
                     ILILLYLKAGFNTLTGLIPGLTIIYFTVFPYYMTKQLVKLKQDGKKLIFFLWDFMMYV
                     FNIQMRNVHYAYDEEVKYHDKKIIKLK"
     gene            9804..12299
                     /locus_tag="ACGEXS_000642"
     CDS             9804..12299
                     /locus_tag="ACGEXS_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8011681.1"
                     /translation="MDLKAPYAGIKNNLLLTKNGEVWAYYRIRSESISTANYDKKEAS
                     KKRMKYFLESIKGYQDFHFEMYEHDLDLRNKFKEISQDFDDSAFDVANYYAHETIDVL
                     EQELQMITRNSFVMGVKLRELSDDAVSIKEILKSTLSDTAERMISNFGFNVSLDEKVL
                     SRYEVYERELADSFLSIEGERLTENELAYIVRKPFISNATHDVQEESSRRAVTDVCNV
                     VIDPTNMRVLKVTNENESFYTTTIVVDDFPLDMEFTHIYEKAQNMPFPVECHVKAKIV
                     ENDKVKKKFDRAKINHKAQAREKQSAGDNASEDIQDNLFVLEQMEKEVTGSGVFIEWS
                     CSFVIKSDDLKDCKRKAKRLIKRLKETNIYAVNPLADQLQLYYQILHGNPLFINKYWV
                     QRTTATGIAENLFGVSQSLGTKTGFYIGRIDKFIRSVSREEAVASSRDIILFSLLLAA
                     KGISGAVSDSPHVLITGQTGKGKSFLAKLLWIYTSFFKGQMIYWDPKSEFADWFNKIT
                     ESEDMQRKYPLFVNHLKTFKYVTLDYKKPSNYGCLDPIVFLDGIEANEMLKSVFDEIK
                     SFENEHRVETAIYRAITETVEEREKGQQVGSLDVIKKLQNDEDEHVSQAGDLLFEKTQ
                     NNILKLVFSDGSNPALNIQEKATILQVQGLDMPKAGDDPSGYSTGEKNGITLMLLIGK
                     FLQKFGSRRQIQTTLFIDEGWAFSASRQGKKVAKGIKRMGRSENNSLVFITQSVKDKA
                     DEDGGNFGCHFAFDEKDEREDILKSLGLEYSKESPENMEMLKDLKKGQCIFSDFYGRV
                     GKMVVHCPFEEMTEAFRTQEDSASSKAEEKFAI"
     gene            12532..13890
                     /locus_tag="ACGEXS_000643"
     CDS             12532..13890
                     /locus_tag="ACGEXS_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFF8011682.1"
                     /translation="MAMNEIALFKGVRIQPYFKHIDFIFAGVFAFIFLFYRILTLWQG
                     YIRQQKALKVVEVYQYFKPHFLYLVIGTLVVFIIAKLFAQLVLRFREKDLAEMIETQG
                     FHNRTVIKKTTEYLDIDYKKTEYDYYPTMFYKRRRKTFMIHVKKDGSRFQDHYLELES
                     IIEPMFNCELVEKRHIGRYIRYEFMPLKYKKRIVMEGTEAPETTYYDTKIYITHQILW
                     DFVKAPHALITGVTGGGKTYFLFYMIRELFKRNAEVRLLDPKVSDLSFMKNVIGAEKV
                     ADTKGQIFKQLREANEEMEHRFRMMSESEHYQLGSNFRNFDLPPYFVIFDEVTAFTST
                     LDKKELQEMNDYLINIIMKGRQAGVFMFLTAQRPDADVIKGNVRDQLGLRVSMGNLSA
                     DGYRMTFGQTDKSFQPIHESDIGRGYISILGQYNEPILFDAPLMEQYDFVADVKQILN
                     KE"
     gene            13897..14064
                     /locus_tag="ACGEXS_000644"
     CDS             13897..14064
                     /locus_tag="ACGEXS_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011683.1"
                     /translation="MNKEQVEQLLSELAKEIGMKSVILIALDLAIKEDNTLRSDEVEK
                     LIKVQRQIEKI"
     gene            14080..15996
                     /locus_tag="ACGEXS_000645"
     CDS             14080..15996
                     /locus_tag="ACGEXS_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFF8011684.1"
                     /translation="MKRKSLYLLCFLILPLFLITGATADAVANPVGKPTNAVDAKIEK
                     YKVDDKADGDIDLERYRIWFQKDEDWNPFGKDEVAETVNNVTNFFFEIDKLIVSITDY
                     AVENLYQLDVLNDFADKVGQFVGDIYQKLLSNIVMTLFIVICLNAFIVFSVKGNMHEA
                     LKRGFLIFCLVGFGVGVLANASFVIKGTNNVGKGLNNVIMNSTSSINGDIDYVNENSG
                     INKIRNQYFDMALYKPYLIMNYGTVNEDKIKQKGKNRIDDILDVKVNSKSDQEKIRDI
                     IEKDEVKKYKNTAMKQTHVFGQLGIAMFTLIITIALSIIFLSISFAKVIFSAFALILF
                     LFLAFSWLISFIPGFEISVFKALARTLGYIILSACMTFLFVVIGFSIDIANAFVKPES
                     QQAYFLNVIFLMIILFVLYKKRSEIIDFVTRGNLSLSPQKLGAEVIRKGQDEFNKRRQ
                     AQAEDKKNKRKNQLDNPEPPMLNNNDNNPKRNQQPDTPISMNNSKEIKRRNQQEEMHG
                     TPNVGLVSYSKPSYSKPSGYTKSKRIAQDDGMHRDVKHKKEIKRNPQTESFSPTYLSQ
                     PPKELSRRSQSFNKDVQSSYDKDIEKRRIQTGEDKPYQPRSHTNEPTFKRNSQTKHVH
                     DRLKNQKDINKHGK"
     gene            15986..17023
                     /locus_tag="ACGEXS_000646"
     CDS             15986..17023
                     /locus_tag="ACGEXS_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HFF8011685.1"
                     /translation="MVNKKTVVKEVMKQTPIGIKFRLLKIGLILFCVLFFLFPVILMF
                     VFSPEEDKQTDGDMSCVGGDVKDKGIAVFEQNAKGGALEGKGKVIVKIAKKHKIPENL
                     FMAIIASESGWGKGENATRQKNPLSVMGTKSIHDSTYPTIEAGLEAGAKNLYDVYIKE
                     GLMTPEKIGPKYAPVGASNDPNNMNKQWIPTVKSMMKKLSDGDGAVCKSTGGKDIKFN
                     GKLPKWSNSDAGKGNLYTAGQCTWYAYGIRQKMGKPISTYWFHAQFWNDRAKDEGYKV
                     NSKPAVGALMIAEPGAGGAPPVTGHVAVVIDVKDDKTFTVTEMNIKGPYIVSQREMKV
                     ESGISFIHDKE"
     gene            17030..17623
                     /locus_tag="ACGEXS_000647"
     CDS             17030..17623
                     /locus_tag="ACGEXS_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011686.1"
                     /translation="MKLKIIGFVIVTILTVSAYFIGTQTTKQQVQKEKNHVSELKQTL
                     QQEKDTNEALNQRVKRQSKTVIAEEEKQIRETAQAFTTELFTLKKGSSFKQKAKPLKP
                     LVTEKYYDVLFSDNREKFSIFDDVTVSDIHVYFDQYDPKHDNYKVFVQFNERIDTEGQ
                     KEVENRKTSVQLDMVRTNDKWLINDVQRFNLQKNGRG"
     gene            17671..18033
                     /locus_tag="ACGEXS_000648"
     CDS             17671..18033
                     /locus_tag="ACGEXS_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystatin-like fold lipoprotein"
                     /protein_id="HFF8011687.1"
                     /translation="MTKRFLLITLILVISTALIACGKKYDKEIEEVTSLEIKDRNDSS
                     DTTFNKYERKKSNIYVYQDGKVIMLSYAPLKGNSELSTRMYLKNETTGKYEKDINGKP
                     KKFMKENKPDYKEENLKE"
     gene            18145..18675
                     /locus_tag="ACGEXS_000649"
     CDS             18145..18675
                     /locus_tag="ACGEXS_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFF8011688.1"
                     /translation="MAKKNTQRKRRVPVDLDTRLAIAKYLDDGLTILQIANILGRHHN
                     VIYYELNHRSQKDGTYDPVKANKMAKETKKMPEKKKQEMRALNPAIKKYIEQKLSLKW
                     SPRQISSEMENDIGYYVSFSTIYRYINTGQVRVNKVKDMRRGGKKYTKTTEERGKIKI
                     GHHRTISSRSNRSSKT"
     gene            18656..19129
                     /locus_tag="ACGEXS_000650"
     CDS             18656..19129
                     /locus_tag="ACGEXS_000650"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFF8011689.1"
                     /translation="MEVVKREEFGHYEIDTIWSKRPSRYCLLTILERKTRFLYAKVLP
                     TRRSDVVCSTIVEILEQENVKTITIDRGKEFAKFELIERELNCSVYFSDPGCPYQRGS
                     IEQVNGLLRQYFPKDTDFVNVSDIDVEIAVLQINQRPRMKQNYKTSEYMRMLEYN"
     gene            complement(19426..19665)
                     /locus_tag="ACGEXS_000651"
                     /pseudo
     CDS             complement(19426..19665)
                     /locus_tag="ACGEXS_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
     gene            20045..20893
                     /locus_tag="ACGEXS_000652"
     CDS             20045..20893
                     /locus_tag="ACGEXS_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HFF8011690.1"
                     /translation="MGNYLVLQSDFGLSDGAVSAMYGVAYTVSDDIRVENLTHDIPPY
                     DIWVASYRLYQTVKYWPKGTVFVSVVDPGVGSDRRSIACLTYSGHYIITPDNGSLSHI
                     KHYEGIKKVIEIDEVKSRLPHSEESHTFHGRDVYAYNGARLAANKIAFERLGQAINVD
                     SIEALEIREVTKNEDSVTGSIDILDIRFGSLWTNIPLAFLKSLEIVHGNNLVVTIYHQ
                     DKKVYQNIIKFARSFADVNVGEPLVYVNSLVNIGVAVNQDSFSDLYHIGTGNDWTIHL
                     SKAPSI"
     gene            20922..21476
                     /locus_tag="ACGEXS_000653"
     CDS             20922..21476
                     /locus_tag="ACGEXS_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HFF8011691.1"
                     /translation="MKKQDISVKTVVAIGIGAAVFVILGRFVVIPTGFPNTNIETSYA
                     FLALISAIFGPFAGLMTGLVGHAIKDFTTYGSAWWSWVICSGIIGCLYGWIGLKLNLS
                     SGRFSRKSMVYFNIGQIIANIICWALIAPTLDILIYNEPANKVYTQGVISAVLNIISV
                     GIIGTILLKAYASSQIKKGSLRKE"
     gene            21529..23241
                     /locus_tag="ACGEXS_000654"
     CDS             21529..23241
                     /locus_tag="ACGEXS_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8011692.1"
                     /translation="MTEPIISFKDFSFQYHSQATPTLQNINVDIYPGEKVLVVGASGS
                     GKSTFANCINGLIPFKTKGNITGELYINNQDATVSCLHDRSNVVGTVLQDTDGQFIGL
                     TAAEDMAFLLENNCVEQDDMKKNVSYWAEKVGMIEHLNHRPQDLSGGQKQRVSLGGIL
                     IHRTPILILDEPLANLDPATGHETLRLLNNIHEETKSTMIIVEHRLEESLDDTFDRVL
                     LFKDGKIIANTTPSDLLKSSKLKEAGIREPLYCTALKYAEVDVESIDNLANLRDVCMS
                     EHVKFKVKKWIDETSANNDNKYKSEPLLELNEVCVQYSDYSNSVLNNVQLNVYRREML
                     SIVGHNGAGKSTLAKAICGFLDITGNIQFCNRGFNQLSISERSEFVGYVMQNPNHMIS
                     EKMIYDEVALGLRARGMKESDIKIRVENVLKICGLYAFRNWPIAALSYGQKKRVTIAS
                     VLVLNPEIIILDEPTAGQDFYHYNEIMSFLIELNRQGKTIIMITHDMHLLSEYSSRTV
                     VLSKGQVVADTTPVLVLNDKKICEIASLRQTSLFEMAEYIGISEPQKLVQLFINHDRK
                     VRRQ"
     gene            23238..24071
                     /locus_tag="ACGEXS_000655"
     CDS             23238..24071
                     /locus_tag="ACGEXS_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFF8011693.1"
                     /translation="MNQYNTIGFHPGNSRIHQLNATVKLLFLLVVSISAMVTYDTRYL
                     ILISASSILLVKYAHIEWKQVRFVVKFILFFTILNIIAVYIFDPEYGVKIYNQRTELV
                     NGIGRFTLTSQELFYLFNLILKYISTVPLALIFLFTTNPSHFAASLNQLGVNYKISYA
                     VSLALRYIPDIQETYFNISQAQQARGYDNSKKAKFTSRVKGIKRIVLPLIFSSIERID
                     TISTAMELRQFGQYKRRTWYVKKQLKKDDYVVLCLTLILLMLVVTLFFLNNSRYFNPW
                     H"
     gene            24118..>24584
                     /locus_tag="ACGEXS_000656"
     CDS             24118..>24584
                     /locus_tag="ACGEXS_000656"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8011694.1"
                     /translation="MINTERLNLMIPSSSHLIELYNICSHPQANIYTPKGLHNSKLDT
                     QRWIEKWRNHWQQYQFGYFVLVKKIDCSVIGICGYEYRQLKQETVLNLFYKLHPSFEG
                     QGYACEAITAITNFVNYIDQETVKVIRTNKCNQRSINLAERLKFKRDDTMDDI"
BASE COUNT         8740 a         3273 c         4626 g         7945 t
ORIGIN      
        1 taaatttttt aatatacgag acatctcttc aattttacca gcatgaagtg ggatgtgaat
       61 ggcatttaat actaataact cttctaagtt attacaaccg acaattggct gtttcaattc
      121 aattgctaaa tcatcttcag ttagatttcg tgcacgttca ggtaaagttt ggagaccttc
      181 taaaatcctt tcaatactcg gtacttcatc cttccattca tctggagacg aaccatttcc
      241 gaataaaggt ctatagatat ttaaatcaat attctcttta ccagcaacag ctaaggccga
      301 ttcaaaaatt gttaatacat gaccaaattg ccaatgaatg gtattagaaa aaggcttatg
      361 ataatcatca agtacctttt caactttcca agcatcatac acatttacta aatatcctaa
      421 ccccatatca atgacatcaa atactgtttt tgtagtcatt attaattcct cccccataaa
      481 taaaatatac ttccgtaagt attaccctat taacaaagtt gttagtcatt taaggaatgt
      541 gtacaacaac taccttacaa taaaagatag ttgacgaaaa gttctcaatg tactatctta
      601 ttatgtaaga tagtaggtga ttaaaatgtc agtgtcagat cagattatga aaggtctctt
      661 agatggggcg atattaggtc tcattgccca aggcgaaacg tatggatatg aaattatgac
      721 gaaacttaag aatcaagagt ttccagagat aagtgagggt agtatttatc cagttttaat
      781 gcgtttaaac aaaaaaggat acgttgatac gacaataaaa aaatctagta atggcggccc
      841 gaggcggaaa tactatacga ttaccgattc aggtttaaag gaactaaaag tatttaaatc
      901 gaaatggcaa gttttgaata aagggatgat taacttgttt ggaggggaaa acgatgttaa
      961 gtaaacaatc tcaagatttt ttagaaaaat tacgtgttga attgttattt agaggtaaga
     1021 ctgaagcaga agtagaagag ttattggaag agctcgaaga tcatttaact atggctgaaa
     1081 aaaataacga agatacaagt tcaattatta atacaccgat taagtcaatg gctgatcaat
     1141 tatcacctga gataagtctt actaccggtt tgtataaata tattactttg tacttcgctt
     1201 ttattttagc agtcattata attcctaggt ttttagattt gggtacattt gatgtgacaa
     1261 ttgcattttt actttatata gctagtatcg ttattttagg tgttgtcatt ggtatgctga
     1321 ttctacgaaa tacattagtc cgatttggtg accaaaaaat aacgtatgtt ataaatgtga
     1381 cttatggaat tatcgtattt ttatggatga ttttaggtgg cttgattatt aaaaaatatc
     1441 ctatctatag tttctttgag ttaagtgaca aacagagttt catcgttggg ttagtacttt
     1501 taattgcgct cattggaact tgtattttaa ttaaacagaa aatttatgcg ctcatcgtgt
     1561 tggtaatttc attgccaagt ttgatagcaa ggatagcagc tatctttagt aaagaaccag
     1621 acacttttaa tttagtttca gtaagtatac tcatcattct aaatatagca tttattattt
     1681 atacatttgt aaattttagg aagttaaaaa aagaagacta aacaatgtcg tttagctaaa
     1741 atacatcatt atagtaaaac tgtataccaa tcatccaaaa gcaccctaaa gttagactta
     1801 aactaacaaa ttagggtgct tttgtttgtg cacttataac tattccgttg tgcccagggc
     1861 taggtgtaaa tcctcatcat tagaaatagc ttcaatggct gcagtcaaac ctgcaactat
     1921 cttttctaat ggcatagatg gtgtatcggg tttaccaatg acctgctctg gtatgtatgg
     1981 cacatgaata aatccgaatc gtaggtgagg gtaatgctta tcttgtaaat aacctaagtg
     2041 ataaagtgtg tgattacaaa caaatgtacc tgcgctattt gaaagtgctc caggaagtcc
     2101 ttgattaata atactttgag tcattgcttt aactggtaaa tttgaaaaat aagctggcgc
     2161 accgtctaag tgaatggctt gatcaatagg ttgaaaatca tcattatctg gaatacgtgc
     2221 atcatcaata ttaatggcga cacgttctgg ggtaatggca tttctaccac cagcttgtcc
     2281 tatagctagt acaacatcat aatgattaga tgccaacgtt ttatttataa tattatctac
     2341 tttcttaaaa gaggttggta gttttaattt atcgattgta tgtgtgccaa taatatcttc
     2401 tagttgagtc acagcttccc atgagggatt gatattttga ttgtcaaaag gcgcaaaccc
     2461 tgttactaaa atgtgcattt taagacaccc cgcagtatta aaatttaatt tagtataaca
     2521 cgcgcataat taaaaacata tcaggttatg tatgcgcata aaaaacagcc ttgaaaaacc
     2581 tttataaatc ggattttcaa agcgtgtttt gaaaggtatt taattaaaac taagcatgtt
     2641 gatgtaaatc atcaaagttt gttaaacgtt gttgccactc attatcacta atattatttg
     2701 cttgaacata acgattacgc tcatgtttag cacattcata agagcatgca cctaaatatt
     2761 tagtttcgtt ttcttcagaa actaatattt gtttattaca ttctgggtta gcgcaattaa
     2821 tataacgttc acatggtttg ccatcaaacc aatccttacc aataattgtt ttttcaactt
     2881 ggttgatatc aacactgata cggtcatcaa atacgtacat tttaccgtcc caatattcac
     2941 cttttgtttc aggatcttta ccatatgtag caataccgcc atgaagttga gctacatctt
     3001 cgaaaccttc ttttaaaagc catccagaaa atttttcgca tcgaatgcca ccagtacagt
     3061 acgtaaccac ttttttatct gcaaataacg ctttattctc tttaatccag tcaggcaaat
     3121 ctctaaaacg tgtgatattt ggacgaattg cacctcggaa atgacctaaa tcaaattcat
     3181 aatcattacg tgcatcaata atgactgtgt catcatcttc aagagctttt ctaaattcta
     3241 caggtgataa atattggcca gttgtgtgtc ttggatcgat gtcatcttct aaatctaaag
     3301 caacgatttc ttttcgagga cgtacatgca ttttcttaaa agcatgtcct tcagcttcat
     3361 caattttaaa caccatatct ttgaatcgtt catcggcatg catatgtgcc atatattgtt
     3421 cggtttcttc ttttgtacca gataatgtgc cgttaatacc ttctgtagaa acaagaattc
     3481 taccttttaa atggtgtgct ttacaaaagg ctaagtgatc ctgagcaaac tgttcagggt
     3541 catcaatcgt catatattta taatataaaa gaacttgata gttcataggt taatctcctt
     3601 ctataaacat gttataagca tgttaatttt taaaataaag cgtcaatacg catatttgtc
     3661 tacgtccatc attttaacag agttgtattg tcattcaata tttctagctt gaaatcagcc
     3721 actagaccga taaaatagat agatgaaacc gttatcattt tatttgatag ttatcttgtt
     3781 taaaataaaa ttgatagtta aaacgaatga aatgaaagga atgatcatga tgtcacaaca
     3841 agatttacct acattatttt atagcgggaa gtccaatagt gctgttccaa ttatatctga
     3901 aagtgaatta caaacaatta cagctgaacc atggcttgaa atttccaaaa aaggattgca
     3961 actagaagga ttgaactttg atcggcaggg acaactcttt ttattggatg tattcgaagg
     4021 caatattttc aaaatcaatc ctgaaacgaa ggaaatcaaa cgaccttttg taagtcacaa
     4081 agcgaatcct gcagcaatca aaatacataa agatggccga ttattcgttt gttatttagg
     4141 agattttaaa tctacaggag gcatttttgc agctacagaa aatggtgaca acttacaaga
     4201 tattattgaa gatctttcaa cagcatattg tattgatgac atggtatttg attctaaagg
     4261 tggattttat tttacagatt ttagaggata ctctaccaat ccactaggag gcgtttatta
     4321 tgtttcgccg gactttagaa cagtgacgcc tatcattcaa aatattagcg tagcaaatgg
     4381 tattgcttta agtacagatg aaaaagtact atgggtaaca gaaacaacag ccaatcgatt
     4441 acatcgcatt gcacttgaag atgatggtgt gacgatacaa ccatttggag ctaccatacc
     4501 gtactatttt acaggtcatg aaggaccaga ctcatgttgt attgatagtg acgataattt
     4561 atacgtagca atgtatggtc aaggtcgagt gttagttttt aataaaaggg gttatccaat
     4621 aggacaaata ttgataccag gccgagatga agggcatatg ttacgttcta ctcatccgca
     4681 atttatacct ggaacaaatc aactcatcat ttgttccaat gatatagaaa tgggcggagg
     4741 atctatgctt tatacagtta atggctttgc aaaaggtcat caaagttttc agtttcaata
     4801 agacctacct gaatgaaagg cgtctaaaat tagggtgaac agttagtgct tgttacacat
     4861 gagttaaacg aaaaatgcca actataccta gggatgatat agttggcatt tcgtatatat
     4921 attaaaacaa tgaggattgt gtaatgagat tattctgaga ttgagaattc agctgatgct
     4981 tcgaatttaa catctttgcc tagcattacg ccaccagttt caagtgcttg gttaaagtta
     5041 atgccatatt tttctctatt gattgtacca gtaacaataa tacctgttac ttgagaacca
     5101 tccataggat tcttacttac tccgttgaat tcaacatcga atgtttcttc gttagtgatg
     5161 cctttaattg ttaaatcacc aacaactttg ctttcagata ctgattttgt cacaaatgta
     5221 attttatggc agtgtaaaat ggttataacg aataattctt aaaaggagtt tgatggttat
     5281 gacgaatttt gcactgtctt tttgtaactc gaataaactg gattatcgaa gttttgttat
     5341 tttgagatat cacggtgttt caaaacgtca tatttgtaag atgtataaaa ttgcatattt
     5401 ttgtatattg gatgtgtgca aaaaagcgaa aaataatgac tatcaattta cttataagga
     5461 ttatgtgttt ttaaaaaaat atggtgtgtc taacgcattt atatgcaaga tgtatcatat
     5521 tgataaatta gacctagaat tctttgaggt gatgaaccga taggtttcac aaaaattaaa
     5581 taggtcagga gaagcataga cactccatta tcaaaaatgc agtggtacgg atttgcggga
     5641 gttattccta ttcgtgcgac cctaaaaaac tctgatttgt atgagtctgc ctattttaat
     5701 taagattgag gtgactttta tgatagaacc agtcattgat attgatgcaa cgatgggtaa
     5761 agagtttcgt tatttatccc atgttgaaaa aatatcacgg tttgatgaag tttattatct
     5821 gtatacaata gtatgtgttg atataggtga acaagttaaa ataagactat ctaatgaaaa
     5881 acactttaat gctttagaac gtgttgattt tgataattta acaattaagc ctagagtata
     5941 tcaagatatg aatacaggtt attgtaattt atatacaagc ttttcagctg atgatattta
     6001 tagtttagaa acaaaagcat aaagagtata aataaaaaat ataaaaagaa atgaggaatt
     6061 tattatggca tggagtccga aatttgattt aaaagaaacg tttggagatt tatattttat
     6121 gggtgttgat gagaaattta aatatgaaga tggagaaaaa actgatcaaa aattatacgc
     6181 atacaaatta gcgtcaactg gtcaaggtga acaagtaact gtaaaagtac ctaaagaagt
     6241 aaaacttgat attatgtcag tttgtgaact tgtaggtgta gaagcaaaac aatatgtaca
     6301 atcttcaggt gattttcatt caattcaacc aagtattaaa gcagaagata ttagacaaat
     6361 tggtacgatt tcagttaaga aaggtgcaca acaaagttag tgtatggaat tttatacagc
     6421 tgataatcta gcgccttatg cacgtacttt aaaattatct gaaggcatgt tgtcatatat
     6481 cgcatctaga attaatacag gtaaagcatt atcattaatg cttatcgcaa aggaaataca
     6541 agaaaaattt aatggcgact atgttaaaag tcgtttacca tcaggcagac cgaggatata
     6601 cactgacgtc tgcttattgt gttttagttt gaaagaagca ggacacgggc gattactaca
     6661 aattgatttg aaggattgta tctatattgg tgatgtagat agttaaatat atcatttgta
     6721 ttgtttaact taaggcgagc tggactttta gcgcctgcag gcgcagaaat gcaagccggg
     6781 acggcgttaa gcatttgggc ggagcctgcg acaaaggcgc gttaagaaag ggaagcgaca
     6841 gccttaagtt atcaaatcgc ttgcgatttg ataggaggtt ttttcatgga aaatcatgct
     6901 tctacccccc ttactaatag gggggtagcg aagccagaaa aatgtggctt agaggcagtt
     6961 gttgattggg ttcaagtgac ttttcaggta ccctctattt tcaccattat ggaggatgtt
     7021 ttgaaactgc ctcgggcagt gtttaaacat cgtaatagtg gtttatatta ttataatcgt
     7081 ggttacacgt ttggtaatat ccaaattttt tattcggata aagatgaagg tatgggtttt
     7141 catttacagt tatctggttc tggttgccga gaatttgaac attatttaat caatcgcaat
     7201 gaaacgtggc aggatttttt taaacgttgt gcgattaaaa aggcgcgttt cacaagaatt
     7261 gatattgcga ttgatgatac aaaaacatat ttaaaaatac cgagactaat caaaaaagct
     7321 gaacgtggtg aatgtatttc aaaatttaga tcagcaagtt caatttctgg gtttaaattg
     7381 tctgatggac agtctaaggg ttcaactttt tatatcggtt ctaaaaaaag tgatatgtat
     7441 tgtcgctttt atgaaaagaa ttatgaaatg gcgtacaaat taaaaacacc tgttgaagat
     7501 tttggtttat ggaatcgtta tgaaatacaa atgcgacgaa gtaatgcgga aaattgtgca
     7561 atgattttaa cagaaacatc aagcatatca gatattgtta aaggtgtttt aaataatagt
     7621 atgcgttttg taactgagcc gaaagatgta tcagatagtc gaaaatcaag gtggccagtt
     7681 tatcaagaat ggtcacgctt tattaaaggt gctgataaaa ttagtttaag tatgaaaccg
     7741 agtttaaagt caattgagga taatattgat tggttatgta aacaagttgc tacaacgtta
     7801 gatactgttt taacagctga agcaatggca caatccgaag gcttacttga agatactgat
     7861 tttctaaata aaatattggc acattcatca tttaatgatg aacataccga tagaattaat
     7921 cattatttag agaatttaaa attaaaaaaa gaacttacgt aaagatgaaa cgtaagtcct
     7981 aaaaaataaa aaaagaaagg atttgatttc tattaaagct tttctaaata atatacgcaa
     8041 agcttttttg aatatcatca acaagttgat actatcaaga ttttcaaaaa aaggcaagcg
     8101 ttttgatgtt ccggaagaag aacgtaaatc attaccgaag tatcgcaatc atagaaaaaa
     8161 gattgtgatt attttttata cgattttagt gttgttaatt attttattga ttgcctcttt
     8221 cattagagct tctaaagcga acaatgaaag taaagaagct gttaataaaa cacatgaaat
     8281 tcaaaagaaa tatgaagata acgctgaaac agttcagtat aatcctaatt taaaattata
     8341 tgctgataag tatattgatg catatatgac tattcctaaa gatggtaagg caaaagaaga
     8401 ccgacaaaaa gtgttagcgt cgttttatcc ttctgattat aagacgccta cagaaaatac
     8461 tgaaaatgtt gagcgtaagc tgaatgaaaa ggaattcttt aatattaaac gtaaagataa
     8521 acaaacgatc attcagtata ttgtgagtta tgatttaaaa attacggaga aaaaagaggt
     8581 caaagttaaa aagaaaaaag ataaaaagga caaagacaaa aaggatgaat atgaaacaaa
     8641 gacagaagaa aaagaacgta cagaaactaa gaaagcatta ttgaacattc ctgttagaag
     8701 tgaaaataat aagtatgtag tgattgaata ccctcatttt tctacgattc caagtaatca
     8761 actgaaaaaa gcaacattgg ttaaagataa tttggaaaat gaaaagcgtg aagataatcc
     8821 aaaattaaaa tcatttgttg aagaattttt tgataaatac gctaaaagca aatcggatga
     8881 catggcttat ttaatggata gtcccgaagg gttagaagat acacgtgaag cgtctaaaat
     8941 cagtgattta cgtatttatc caaaaggtga tgattatgtt gttaaagcag aggttttaat
     9001 gaaagataaa gggtcgccac ttgaaaactt agaacattac accttagata ttacgaaaaa
     9061 agatggtaag tattatgtga aaaagttaac aactacaatt ggaggttaaa gtgatggaac
     9121 atttatttaa tttaatgttg gtattaggtg atgcaccaac tattaatcca cttggtcaat
     9181 ggattaataa agaagttggt accgcaattg gtatcattgt attggtgatt ggttgtgtta
     9241 aatgggcgac cggtaagtat gggcatatgc ttgcattgtt tgttattggt ggtttaatgt
     9301 tcctgatttc taaaggtcct gagacagtgt ttaattcagt gtcaggaatt tggaaattaa
     9361 tttttgggag ctgatcgata tggataaaaa agcatataac cttaaacgaa catttgaaca
     9421 gccgattgtg atgtatgaat ttacaggaag ttatcggtgg tcaaaaggta ttcgtttaga
     9481 ctgggtcggt acgttgtttg tgattgaatt gattttaata ttattatatc ttaaggctgg
     9541 atttaataca ttaacaggtt taataccagg tttgacgatt atttatttta cagtattccc
     9601 ctattatatg acgaagcaat tagtaaaatt aaaacaagat ggtaagaaac tgatattctt
     9661 tctttgggat tttatgatgt atgtgtttaa cattcaaatg cgcaatgtac attatgcata
     9721 tgatgaagaa gtgaaatatc atgacaagaa aataatcaaa ttaaaatagt ccctaacggg
     9781 gctattttta atggagggat gacatggatt taaaagcccc ctatgcaggg ataaaaaaca
     9841 atttgttatt aacgaaaaac ggtgaagttt gggcgtatta tcgtattcgg tctgaatcta
     9901 tttcaacagc caattatgat aaaaaagaag cgtcaaaaaa acgaatgaag tattttcttg
     9961 aatcgattaa aggctatcaa gattttcatt ttgagatgta tgaacatgat ttagatttac
    10021 gtaataaatt taaagaaatt agtcaagact ttgatgattc agcgtttgat gttgccaatt
    10081 attatgccca tgaaacgatt gacgtgttag aacaagaatt acaaatgatt acacgtaact
    10141 cgtttgtaat gggtgtgaag cttcgagaat tgagtgatga tgcagtttcg attaaagaaa
    10201 tccttaagag tacattatca gatacagcgg agcgtatgat ttcgaatttt ggttttaatg
    10261 tctcattaga tgaaaaagta ttaagtcgct acgaggttta tgaacgagaa cttgcggata
    10321 gttttttaag tattgagggt gaacgattaa ctgaaaacga acttgcttat attgtgcgaa
    10381 aaccatttat ttcaaatgcg acgcatgatg tacaagaaga atcatcaaga cgtgctgtta
    10441 ctgatgtatg taatgtggtt attgacccta caaacatgcg tgtattgaaa gtgacaaatg
    10501 aaaatgaatc gttttataca acaacgattg ttgttgatga ttttccactt gatatggaat
    10561 ttacacatat ttatgaaaaa gcacaaaata tgcccttccc tgtggaatgt cacgtgaaag
    10621 caaaaattgt tgaaaatgat aaagtgaaaa agaaatttga tcgtgcaaaa attaatcata
    10681 aagcacaggc aagagaaaaa caatcagctg gtgacaatgc atctgaagat attcaagata
    10741 atttatttgt attagaacaa atggaaaaag aagtgacggg ttcaggtgta tttatagaat
    10801 ggtcttgttc atttgtgatt aagagtgatg atttaaaaga ttgtaagcgt aaagcgaaac
    10861 gtttaattaa acgtctgaaa gaaacaaata tttatgcagt gaatccccta gctgaccaat
    10921 tacaattata ttatcaaatt ttacatggca atccattgtt tattaacaaa tattgggtgc
    10981 aacgtacgac agcaacaggg attgcagaaa acttatttgg tgtgtctcaa agtttaggta
    11041 caaaaactgg tttttatatc ggacgtattg ataagtttat acgatctgtt tctcgtgagg
    11101 aagccgttgc aagttcaaga gatattattt tatttagttt attattagca gccaaaggga
    11161 tatcaggagc agtcagtgat agcccacacg tattaattac tggtcaaaca ggtaaaggta
    11221 aatcattctt agctaagtta ctttggatat atacatcatt ctttaaaggt caaatgattt
    11281 attgggaccc gaaaagtgaa tttgcagatt ggtttaataa aattacagaa agtgaagaca
    11341 tgcaacgtaa ataccctcta ttcgtcaatc atttaaaaac atttaagtat gtgacattgg
    11401 attataaaaa acctagtaat tatggttgtt tagatccgat tgtttttctt gatggtatcg
    11461 aagcaaatga aatgttgaaa tcagtgtttg atgaaattaa atcgtttgaa aatgaacatc
    11521 gtgttgaaac agcgatttat agggctatta ctgaaacagt tgaggaacgt gaaaaaggtc
    11581 aacaggttgg gtcattagat gtaattaaaa agcttcaaaa tgacgaagac gaacatgtaa
    11641 gccaagctgg tgatttactt tttgagaaaa cacaaaataa tatattaaaa cttgtgttta
    11701 gtgatggtag taatccagct ttgaatattc aagaaaaagc aacgatatta caagttcaag
    11761 gcttagatat gccgaaagct ggtgatgacc catcgggtta ttcaacaggt gaaaaaaacg
    11821 ggattacttt aatgctttta atcggtaaat tcttacagaa attcggttca cgcagacaga
    11881 tacaaacaac tttatttatt gatgagggct gggcatttag tgcgtcacgc caaggtaaaa
    11941 aagtagctaa aggcattaaa cgtatgggtc gatcagaaaa caattcattg gtttttatta
    12001 cccaatctgt taaagataaa gccgacgaag acggcggtaa ctttggttgt cattttgcgt
    12061 ttgatgaaaa agatgagcgt gaggacatct taaaatctct tggtttagaa tattcaaaag
    12121 aaagtcctga aaatatggaa atgttgaaag atttgaagaa aggtcaatgt attttcagtg
    12181 atttctatgg acgtgtaggc aaaatggttg tgcattgtcc atttgaagaa atgacagaag
    12241 catttagaac acaagaagat tcagcaagtt caaaagcaga agagaaattt gcgatataag
    12301 gtgatgtgta tgttgattat attgaaaata ttgattttag tagttttttc aatagtttat
    12361 ttaactttgt tgtttttatg gtatgagaca gaatcttgga agttacttat atctattttt
    12421 ttattaattt cattagttat gttgtatatt aaatatttca ctgatggtca tagttatcca
    12481 gttatttttt aaacagtata tttatttgag acttttgaaa ggagtgattg aatggcaatg
    12541 aatgaaattg cgttatttaa aggtgtgcgt attcaaccgt attttaaaca tattgatttt
    12601 atttttgcgg gtgtattcgc gtttatcttt ttattttatc gaatattaac gttatggcaa
    12661 ggttatattc gacaacaaaa agctttaaag gtggttgaag tttaccaata ttttaagcca
    12721 cattttttat atcttgtgat tggtacattg gttgttttta ttatcgcaaa attatttgcg
    12781 caacttgttt taagatttcg cgagaaagat ttagctgaaa tgattgaaac acaagggttt
    12841 cataatcgta cagtgattaa gaaaacaacg gagtatttag atattgatta taagaaaaca
    12901 gaatatgatt attatccaac aatgttttat aaacgtagac gtaaaacatt tatgatacat
    12961 gttaaaaaag atggttcgcg ttttcaagac cattacttag aattagaatc aatcattgaa
    13021 ccaatgttta attgtgagtt ggttgagaag cgtcatattg ggcgttatat tagatatgag
    13081 tttatgcctc ttaaatataa aaagcgtatc gttatggaag gcacagaggc gccagagaca
    13141 acgtattatg atacaaagat ttatattacc caccaaatcc tttgggattt tgtgaaagca
    13201 ccacatgctt taattactgg tgtgaccggt ggcggtaaaa cgtacttctt attttacatg
    13261 attagagagt tatttaagcg caatgcagaa gtacgattgt tagaccctaa agtatctgat
    13321 ttatcattta tgaaaaatgt aattggtgct gaaaaagtgg cagatacgaa aggacagata
    13381 tttaaacaat tacgagaagc caatgaagaa atggaacacc gttttagaat gatgagtgaa
    13441 agcgaacatt atcaattagg tagtaatttt agaaattttg atttaccacc ctattttgtg
    13501 atttttgatg aagtgacagc attcacgtct actttagata aaaaagaact tcaagaaatg
    13561 aatgattatt taattaatat cattatgaaa ggtcgtcaag ctggtgtatt tatgttttta
    13621 acagcacaac gacctgatgc agatgtgatt aaaggtaatg tgcgtgacca attgggctta
    13681 cgtgtgtcaa tgggtaatct ttcagctgac ggttatcgta tgacttttgg tcaaacagat
    13741 aaatcatttc aaccgattca cgaatcagat attgggcgtg gttatatttc aatacttgga
    13801 caatataatg aaccgatttt atttgatgcg ccactgatgg aacaatatga ttttgtggca
    13861 gatgtgaaac aaatattaaa taaggaatga taaataatga ataaagaaca agtagaacaa
    13921 ttgttaagtg aattggctaa ggaaattgga atgaaaagcg ttattttaat agctttagat
    13981 ttagcaatta aagaggataa tacattacgt tctgatgaag tggaaaaact aattaaagta
    14041 cagcgtcaaa ttgaaaaaat ttaaataagg agtggttgaa tgaagcgtaa aagtttatat
    14101 cttttatgct ttttaatttt gcccttgttt ttaataacag gggcaacggc tgatgccgtg
    14161 gctaatccag taggaaaacc aacgaatgcg gtagatgcga agattgaaaa gtataaagtg
    14221 gatgataaag ctgatggtga tatagattta gaacgttatc gtatatggtt tcaaaaagac
    14281 gaagattgga atcctttcgg taaagatgaa gtcgcagaaa cagtcaataa tgtaacgaat
    14341 ttcttttttg aaattgataa gttgatagta agtattactg attatgcggt agaaaattta
    14401 tatcagcttg atgtattaaa tgattttgcg gataaagtcg gtcaatttgt tggagatatt
    14461 taccagaagt tattaagtaa tattgtcatg actttgttta ttgtgatttg tttaaatgcg
    14521 tttattgttt tttcggttaa aggtaatatg catgaagcat taaaacgtgg ttttttaatt
    14581 ttttgtttag ttggttttgg tgttggcgtc cttgcgaatg ccagctttgt gattaaaggt
    14641 acaaataatg ttggtaaagg cttaaataat gtcattatga atagtacaag ttccattaat
    14701 ggtgatattg attatgtaaa tgaaaattca ggcattaaca agatacgtaa tcaatatttt
    14761 gatatggcac tgtataagcc ctatcttatt atgaattatg gcactgtgaa tgaagataaa
    14821 attaagcaaa aaggtaaaaa tcgtatcgat gatattttag atgttaaagt taattcaaaa
    14881 tctgatcaag agaaaataag agatattatt gaaaaagatg aagtgaaaaa atataaaaat
    14941 acggcaatga aacaaacaca tgtatttggg caactaggta ttgcgatgtt tacgttgatt
    15001 attacgattg ctttatctat tatatttctt tcgattagtt ttgcgaaagt catcttttca
    15061 gcttttgcgc tcattttatt cttattttta gcgtttagtt ggcttattag ttttattcct
    15121 ggttttgaaa tatcggtatt taaagcatta gcacgtacat taggttatat tattttaagt
    15181 gcgtgtatga catttttatt tgtggtgata ggtttttcaa ttgatatcgc aaatgcattt
    15241 gtgaagcccg aaagtcaaca agcttatttt ttaaatgtga tattcttaat gattattttg
    15301 tttgtattgt ataaaaaacg tagtgaaatt attgattttg tgacacgtgg caatttaagt
    15361 ttatcccctc aaaaattagg cgcagaggta atacgtaaag gacaagatga atttaataag
    15421 cgtcgtcaag cacaagcaga agataaaaag aataaacgta aaaatcaact tgataatcca
    15481 gaaccaccga tgttaaataa caatgataat aatccaaagc gaaatcaaca accagatacg
    15541 cctatttcca tgaataattc gaaagaaatt aagcgacgta atcaacaaga agagatgcat
    15601 ggcactccta atgtaggttt agttagttat tcgaaaccat cttattcaaa accgtcaggt
    15661 tatacgaaat caaaacgaat cgcacaagat gatggtatgc atcgtgatgt aaaacataaa
    15721 aaagaaatta aacgtaatcc gcaaacagaa tcatttagcc ctacttattt atctcaaccg
    15781 ccaaaagaat tatcaagacg tagtcaatcg tttaataaag acgttcaatc ttcttatgat
    15841 aaagatatcg aaaaacgtcg tatacagact ggagaagata aaccatatca accacgttca
    15901 catacaaatg agccgacatt taaacgaaat tcacaaacaa aacatgttca tgatcgattg
    15961 aaaaatcaaa aggatattaa taaacatggt aaataaaaag acggttgtaa aagaagtgat
    16021 gaaacaaacg ccgattggta ttaaatttag attattaaaa attggtctga tattgttttg
    16081 tgtgttgttt ttcttgttcc ccgtgatatt gatgtttgta tttagtccag aggaagacaa
    16141 acaaacagat ggtgatatgt catgtgtggg tggtgatgtt aaagataaag gtattgcggt
    16201 atttgaacaa aatgcgaaag gtggcgcatt agaaggtaaa ggaaaagtga ttgtgaaaat
    16261 cgcgaaaaag cataaaatac ctgagaattt atttatggcc attattgcga gtgaatccgg
    16321 ttggggtaaa ggtgaaaatg cgacgcgtca aaagaatccc ttatctgtaa tgggtacaaa
    16381 gtcgattcat gattctacgt atccaacaat tgaagctgga cttgaggcag gcgcaaagaa
    16441 tttatatgat gtttatatta aagaaggatt aatgacacca gagaaaattg ggccaaaata
    16501 tgcgcctgta ggtgcttcaa atgacccgaa taatatgaat aagcaatgga taccaacagt
    16561 aaaatcaatg atgaaaaaat taagtgatgg tgatggcgct gtatgtaagt ctactggtgg
    16621 taaagatatt aagtttaatg ggaagttgcc aaaatggagt aattcagatg caggtaaagg
    16681 taatttatat acagcgggtc aatgtacatg gtatgcctat ggcattcgtc aaaaaatggg
    16741 aaaaccgata tcgacgtatt ggttccatgc gcaattttgg aatgatagag cgaaagatga
    16801 aggttataaa gtaaatagta aacccgctgt aggtgcgtta atgattgccg agcctggcgc
    16861 tggtggtgca ccgccagtaa caggacatgt agctgttgtg attgatgtga aagatgataa
    16921 aacatttact gtgacagaaa tgaatattaa aggaccttat atcgtatcac aaagagaaat
    16981 gaaagtagaa tctggtatta gttttattca tgataaggag tagataataa tgaaattaaa
    17041 gattattggc tttgtgatag tgaccatttt aacagtgagt gcttatttta taggtacgca
    17101 aacaacaaaa caacaagttc aaaaagaaaa aaatcatgtg agtgaattaa aacaaacatt
    17161 acaacaagaa aaagatacga atgaggcatt aaatcaacgt gtaaaacgtc aatctaagac
    17221 agtgattgcg gaggaagaaa aacaaatacg tgagacagca caagcattta caacagagct
    17281 atttacgtta aaaaaaggtt catcatttaa acaaaaagct aaaccactta aaccactagt
    17341 aacagaaaaa tattatgatg tattgtttag tgataataga gagaagttta gtattttcga
    17401 cgatgtaaca gtgagtgata ttcatgtata ttttgatcag tatgacccta agcatgataa
    17461 ttataaagta tttgtgcaat ttaatgagcg tattgatact gaggggcaaa aggaagttga
    17521 gaatcgtaaa acatctgtac agcttgatat ggtacgtaca aatgataaat ggttaattaa
    17581 tgatgtacag cgttttaatt tacaaaaaaa tgggcgtgga taatttataa aataatgata
    17641 taattcaata aaaaaggaga tgattatatc atgacaaagc gatttttatt gataacattg
    17701 attttagtta tatctacagc tttaattgca tgtggtaaaa agtatgataa agaaatagaa
    17761 gaagttacta gtttagaaat aaaagataga aatgattcat ctgatacaac ctttaataaa
    17821 tatgaaagaa agaaaagtaa tatttatgtg tatcaagatg gtaaagttat tatgttaagt
    17881 tatgcacctt taaaaggaaa ctcagagtta agtacacgta tgtatttaaa aaatgaaact
    17941 acaggaaaat atgaaaaaga tattaatggt aaaccaaaga aatttatgaa agaaaataag
    18001 cccgattata aagaggaaaa tttaaaagaa tagacaaaag caactcccta aagcgaatga
    18061 aaagtttggg agttgctttc ttttaacatc ttcttctatt atattttata aacgatgttt
    18121 aaaaatgtaa aggagaagat gaaaatggct aaaaagaata cgcaacgaaa gagaagagtg
    18181 ccagtagatt tagacacgcg tttagcaatt gcgaaatatc ttgatgacgg attaactata
    18241 ttacaaattg cgaatatatt aggtagacat cataatgtga tttattatga gttgaatcat
    18301 cgttctcaaa aagatggaac atatgaccct gtaaaagcaa ataaaatggc taaagaaact
    18361 aagaaaatgc cagaaaaaaa gaagcaagag atgcgtgcat taaatcctgc tataaagaaa
    18421 tatattgagc agaaattgtc tttaaagtgg tcacctagac aaatttcttc agaaatggaa
    18481 aatgatattg gctattatgt tagcttttct actatatata gatatattaa tactggacaa
    18541 gttagagtaa ataaagtaaa agatatgcgc cgaggtggaa aaaagtatac aaaaacgact
    18601 gaagaacgtg gcaaaattaa aataggtcat catcgtacaa taagtagtcg gtccaataga
    18661 agtagtaaaa cgtgaagaat ttggacatta tgaaatagat actatttgga gtaaacgtcc
    18721 ttctcgatat tgcttattaa cgattttaga acgtaaaaca agatttctat atgcgaaagt
    18781 tttacctaca agacgttctg atgttgtatg ttcaactatt gtagaaatat tggaacaaga
    18841 aaatgttaaa acaattacga ttgatagagg taaagaattt gcgaagtttg aattaattga
    18901 acgtgagttg aattgttcag tttattttag cgatccgggt tgtccatatc aacgtggttc
    18961 tatagaacag gtgaatggat tattacgtca atattttcct aaagatacag attttgtaaa
    19021 tgtttctgat atagatgtag aaatagctgt tctccaaatt aatcagcgcc caagaatgaa
    19081 gcaaaattat aaaacaagtg agtatatgag aatgttagaa tataattaga gacgctcata
    19141 accctaatga ccttactttt tagtgattgt gttctgtaag aacctatcac taaaaataag
    19201 gtggtctaag cgagcgttta aacgcgagtg cggaagtgaa aaggtttggc ataatgtata
    19261 agtaatgtat ataaacatta taattgtaac ccttttctta gttagcagtt ttgtgataca
    19321 atgttagcaa taataatcaa ttaatatatc ttctatttat aatctttctt tataaccatg
    19381 aaaatttatt ttaaaaattt ttcaaaacct cttgacacac tgcatatcaa attcgtcagt
    19441 accaaagaaa tcaccagatt ttaagtggtt atcacgtgct tcgtttttag tgttaattga
    19501 gcttggaata attgttgcag tagctttcaa agtactgaag tcattaatat ctccttcaac
    19561 agctacatca aattgatcaa atgaaccttt cactttagaa accattaaat gtttaatttg
    19621 gaattctaaa ctactgtgtg caccatcaaa agtaaagtta gtcataatat aaaacctcca
    19681 tatttttatc tcttagatat attttttata cttgatatac atacaatacc acctaaaaaa
    19741 caaagatgca agcattttat ttcgaattcg aatattttct gtgatgtatt taaagaatga
    19801 cctataaaac accacactat tgttataccc tagtcaatta attcaaacat aaagaagtga
    19861 ttacaagtcc gtgaaattta gttaatgaga taaatctgac tattattatc atttttatag
    19921 tcactactat tgactttgca tcaagtgact cgtactattt taatataatt ctgaaaattc
    19981 aaaatataga acattaaata tgtagataat atttttttga agttttaaaa aaaggaggca
    20041 ttttatggga aattatttgg tattgcaatc agattttgga cttagtgatg gcgctgtaag
    20101 tgcaatgtat ggagttgcat atactgtaag tgatgatata cgtgttgaaa acttaacgca
    20161 tgatatacca ccgtatgaca tttgggtagc gtcatatcgt ttatatcaaa ctgtaaaata
    20221 ctggcctaaa ggcacagttt ttgtatcagt ggtagacccg ggtgtaggta gtgataggcg
    20281 gagcattgct tgcttaacat attcaggtca ttacattatt acacctgata atggttcttt
    20341 aagtcatatt aaacattacg aaggtattaa aaaagttatc gaaattgatg aagtgaaaag
    20401 ccgattgcct cattcggaag aaagtcatac ttttcatggt agagatgttt atgcatacaa
    20461 tggtgcgaga ttagctgcaa ataagatagc gtttgaacgt ctcggtcagg caattaatgt
    20521 tgatagtatt gaggcattag aaataagaga agtgacaaaa aatgaggatt ctgttacagg
    20581 aagcattgat attttagata tcagatttgg gtcattatgg acaaatattc cattggcatt
    20641 cttaaaatcc ttggaaattg ttcatggtaa taatttggtg gtaacaattt atcatcaaga
    20701 taagaaagtc tatcaaaata ttattaaatt tgcgcgatcg tttgcagatg ttaatgtggg
    20761 tgaacctctt gtgtatgtta actcgctagt aaatattggt gtagcagtca atcaagactc
    20821 attttcagat ttatatcata ttggtactgg aaatgattgg acgattcatt tatctaaagc
    20881 accatcaata taatgaattt tttatgtaag gagtaatgaa gatgaaaaaa caagatattt
    20941 cagttaaaac tgttgttgca attggtatag gagcagcggt atttgtaatt ttagggcgtt
    21001 ttgttgtaat accaacaggt tttcctaata cgaatataga aacatcatat gcatttttag
    21061 cattaatatc tgcaatattt ggaccttttg ctggtttaat gactggatta gttggacatg
    21121 ctattaaaga ttttacgaca tacggtagtg cctggtggag ttgggttatt tgctcgggaa
    21181 ttataggctg tttatatggc tggattggac taaaattaaa tctttcttca ggccgatttt
    21241 ctaggaaatc gatggtttat tttaatatcg gtcaaattat tgcgaatatt atttgctggg
    21301 cacttattgc accaacatta gatattttga tttataacga accagctaac aaggtttata
    21361 cacaaggtgt tatctctgca gtattaaata ttatttcagt tggcattatt gggacaatat
    21421 tattaaaagc atatgcttca tctcaaatta aaaaaggtag tttacgtaag gaatagttat
    21481 tttgttgaat cagatatgta aatgaatata gaaaggtaat gatatatcat gactgaacca
    21541 attatctcgt tcaaagactt tagttttcaa tatcatagtc aagcaacacc tacattacag
    21601 aatataaatg ttgatattta tccaggagaa aaagtattag tagttggtgc ttcgggtagt
    21661 ggtaaatcga cttttgcaaa ttgcataaac ggattaattc catttaaaac taaaggtaac
    21721 ataactgggg aactatatat aaataatcaa gatgcaaccg ttagttgttt acatgataga
    21781 tctaatgttg ttggtacagt tttacaagat acagatggac agttcatagg cttaacagca
    21841 gctgaagata tggccttttt attagaaaat aattgtgttg aacaagatga tatgaagaaa
    21901 aatgtaagtt attgggctga aaaagttggc atgatagaac atttaaatca ccgaccgcaa
    21961 gatttatctg gaggtcaaaa acaacgcgtt tcattaggtg gtatattaat ccatcgtacg
    22021 cctattttaa tattggatga gccactggcc aatttagatc ctgcgacagg acatgaaacg
    22081 ctgagattgt taaacaatat tcatgaagaa acaaagtcaa cgatgattat tgtcgaacat
    22141 cgattagaag aatcattaga tgatacgttt gatagagttc ttctttttaa agatggaaaa
    22201 atcatcgcta atacaacgcc tagtgatttg ctaaaatcat ctaagcttaa agaagcaggc
    22261 atacgtgaac cattatactg tacagcgctt aaatatgcag aagtagatgt cgaatccatt
    22321 gataacttag cgaatttacg tgacgtttgt atgagtgaac atgttaaatt taaagtgaaa
    22381 aaatggatag atgaaacatc tgcaaataat gataataagt acaaatctga accgctatta
    22441 gaattaaatg aggtatgtgt tcaatatagt gactatagta atagtgtact taataatgtt
    22501 caattaaatg tttatagaag agaaatgctt agtatagttg gtcacaatgg tgccggcaaa
    22561 tcaacacttg ctaaagcaat ttgtggattt ttagatataa caggtaatat ccaattttgt
    22621 aataggggat ttaatcaatt gtcaattagc gaacgatctg aattcgtagg ttatgtgatg
    22681 caaaatccaa atcatatgat ttctgaaaaa atgatttacg atgaagtagc attagggtta
    22741 agagcacgag gtatgaaaga atcagatata aagatacgag tagagaatgt gctcaaaata
    22801 tgcggacttt atgcatttcg gaattggcca attgcagctt taagctatgg tcaaaaaaag
    22861 agggtcacta tagcatctgt tttagtctta aatccggaaa taatcatatt ggatgaaccg
    22921 actgctggtc aagatttcta tcattataat gagataatgt catttttaat tgaactaaac
    22981 agacagggga agacgattat tatgattacg catgatatgc atttattgtc tgagtatagt
    23041 tcaagaacag ttgtattatc aaaaggtcaa gtcgttgctg ataccacgcc agtattggtt
    23101 ttaaatgata aaaaaatctg tgagattgca tcattgagac aaacatcgct atttgaaatg
    23161 gccgaatata tagggattag cgagccacag aaattagtac aattatttat taaccatgat
    23221 aggaaggtga gacgccaatg aatcaatata atactatagg ttttcacccg ggaaatagtc
    23281 gtattcatca attaaatgcg actgttaaac ttttattctt attagttgtt tctatttctg
    23341 caatggtgac ttatgacaca agatatttaa ttttaattag tgcttcatct attttattgg
    23401 tcaaatatgc tcatattgaa tggaaacaag ttcgctttgt tgttaaattc attctgtttt
    23461 tcacaatatt aaatattatt gccgtgtaca tatttgaccc tgaatatggt gtgaagattt
    23521 ataatcagcg tacagagtta gtcaatggta ttggtcgatt tacgctaaca tcacaggaat
    23581 tattctatct ttttaatcta atattaaaat atattagtac agttccttta gcgttaatat
    23641 ttttattcac aacgaatccg agtcattttg ctgcaagttt aaatcagcta ggtgtgaatt
    23701 ataaaatcag ttacgcagtc tcactagcat taaggtatat tccagatatt caagaaacat
    23761 attttaatat ttcacaagcg caacaagcaa gaggatatga taattcgaaa aaagcaaaat
    23821 ttactagccg tgttaaaggt atcaaacgca ttgtgttacc tttaatattt tctagtatcg
    23881 aaagaattga cactattagt actgctatgg agttaagaca attcggacag tataaaagga
    23941 gaacctggta cgtcaaaaaa caattaaaaa aagatgatta tgttgttttg tgtttgacgt
    24001 taatacttct gatgttagta gttacattat tctttttaaa taatagtcga tatttcaacc
    24061 cgtggcatta gtattcatat aaatagtctt taaatagaaa taggagggag acatttaatg
    24121 ataaatactg aaagattaaa tttaatgatt ccaagttcct cgcatttaat tgaactttat
    24181 aatatttgta gtcatccaca agcaaatata tacactccca aaggtttaca taattccaaa
    24241 ttagacacac aacggtggat tgaaaaatgg cgaaaccatt ggcaacaata tcaatttggt
    24301 tactttgtat tggtaaaaaa aatagattgt agtgttattg gtatttgtgg atatgaatat
    24361 cgacaattaa agcaagaaac agtattaaat ttattttata aattacatcc aagttttgaa
    24421 ggacaagggt acgcatgtga ggctattaca gcaatcacaa attttgtgaa ttatatcgat
    24481 caagaaacag taaaagttat caggacaaat aagtgtaacc aacgttcaat aaatttagca
    24541 gaaaggctta aattcaagcg agacgatact atggacgaca ttat
//
LOCUS       DAXWFK010000020        24575 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.020, whole genome shotgun sequence.
ACCESSION   DAXWFK010000020 DAXWFK010000000
VERSION     DAXWFK010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 24575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24575
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.020"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            111..1187
                     /locus_tag="ACGEXS_000657"
     CDS             111..1187
                     /locus_tag="ACGEXS_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFF8011695.1"
                     /translation="MNQSVKLLKHLTDVNGIAGYEMQVKEAMRNYIEPVSDQIIEDNL
                     GGIFGKKNAENGQYSIMISGHMDEVGFMVTKIDKHGFISFTPVGGWWNQVMLSQKVTI
                     TTDSGKEIRGIIGSKPPHVLTPEERKKPMEIKNMFIDIGVSSKEEAEEAGVEVGNMVT
                     PYSEFEVLANDKYLTAKAFDNRYGCALAIEVLKRLKDENIGINLYSGATVQEEVGLRG
                     AKVAANTIKPDLAIAVDVGIAYDTPGMSGQTSDSKLGGGPVVIMMDATSIAHQGLRKH
                     IKDVAKEHNIEVQWDTTPGGGTDAGSIHVANEGIPTMTIGVTLRYMHSNVSVLNVDDY
                     ENSIRLVTEIVRSLNDESYKNIMW"
     gene            1435..2097
                     /locus_tag="ACGEXS_000658"
     CDS             1435..2097
                     /locus_tag="ACGEXS_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFF8011696.1"
                     /translation="MLLEIKDLVYKASDRIILDHISLKVDKGESIAIIGPSGSGKSTF
                     QKQICNLFSPTSGELYFKGKPYNDYDPEELRQRISYLMQQSDLFGETIEDNMIFPSLA
                     RNDKFDRKRAKQLIKDVGLGHYQLSSEVENMSGGERQRIAIARQLMYTPDILLLDEST
                     SALDVNNKEKIENIIFKLADQGVAIMWITHSDDQSMRHFQKRITIVDGQISNTEELNQ
                     HE"
     gene            2090..2866
                     /gene="fetB"
                     /locus_tag="ACGEXS_000659"
     CDS             2090..2866
                     /gene="fetB"
                     /locus_tag="ACGEXS_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFF8011697.1"
                     /translation="MSNTALGLTALLLVIPIIISYKEGLHIIKDLIVATLRAVVQLII
                     LGFLLHYIFKINDKWLLILCVLVIIINASWNTISRASPVMHHVFWISFLAIFIGTALP
                     LAGTIATGAIQFTANEVIPIGGMLANNGLIAINLAYQNLDRAFVQDGTNIESKLSLAA
                     TPKLASKGAIRESIRLAIVPTIDSVKTYGLVSIPGMMTGLIIGGVPPLQAIKFQLLVV
                     FIHTTATIMSALIATYLSYGQFFNARHQLVARNTDVKSES"
     gene            3262..4449
                     /locus_tag="ACGEXS_000660"
     CDS             3262..4449
                     /locus_tag="ACGEXS_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8011698.1"
                     /translation="MSIMRLFTFILSIFIVGMVEMMVAGIMNLMSQDLHVSEAVVGQL
                     VTMYALTFAICGPILVKLTNRFSSRPVLLWTLLIFIIGNGIIAVAPNFSILVVGRIIS
                     SAAAALIIVKVLAITAMLSAPKNRGKMIGLVYTGFSGANVFGVPIGTVIGDLVGWRYT
                     FLFLIIVSIIVGFLMMIYLPKDQEIQRGPVNHETPSHENHVTSKILRPAEVAKYLIIT
                     FLVLIANSVTFVFINPLILSNGHDMSFVSLALLVNGIAGVIGTSLGGIFSDKITSKRW
                     LMISVSIFIVMMLLMNLILPGSGLLLAGLFIWNIMQWSTNPAVQSGVIQHVEGDTSQV
                     MSWNMSSLNAGIGVGGIIGGLVMTHVSVQAITYTSAIIGALGLIVVFTLKNNHYAKTF
                     KSS"
     gene            complement(4511..5863)
                     /locus_tag="ACGEXS_000661"
     CDS             complement(4511..5863)
                     /locus_tag="ACGEXS_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HFF8011699.1"
                     /translation="MKINTTGGQIHGITQDGLDIFLGIPYAEPPVHDNRFKHSTLKTQ
                     WSEPIDATEIQPIPPQPDNKLEDFFSSQSTTFTEHEDCLYLNIWKQHNDQTKKPVIIY
                     FYGGSFENGHGTAELYQPAHLVQNNDIIVITCNYRLGALGYLDWSYFNKDFHSNNGLS
                     DQINVIKWVHQFIESFGGDANNITLMGQSAGSMSILTLLKIPDIEPYFHKVVLLSGAL
                     RLDTLESARNKAQHFQKMMLDYLDTDDVTSLSTNDILMLMAKLKQSRGPSKGLDLIYA
                     PIKTDYIQNNYPTTKPIFACYTKDEGDIYITSEQKKLSPQRFIDIMELNDIPLKYEDV
                     QTAKQQSLAITHCYFKQPMKQFLQQLNIQDSNAQLWLAEFAWHDTSSAHYRSAYHILD
                     MVFWFGNLQILAAHQYPTTAHLKFLSRQMQNDLANFAKSGKMPWPMYHNERRYYRTYQ
                     "
     gene            complement(6471..8108)
                     /locus_tag="ACGEXS_000662"
     CDS             complement(6471..8108)
                     /locus_tag="ACGEXS_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFF8011700.1"
                     /translation="MNKDKQLHNDKINLSQLVLLGLGSLIGSGWLFGAWEASSIAGPA
                     AIISWVLGFLVIGTIAYNYIEIGTMFPQSGGMSNYAQYTHGSLLGFIAAWANWVSLVT
                     IIPIEAVSAVQYMSSWPWHWAKPMRYLMENGSISTYGLLAVYLIIVIFSLLNYWSVKL
                     LTSFTSLISVFKLGVPMLTIIMLMLSGFDTSNYGHSASTFMPYGSAPIFAATTASGII
                     FSFNSFQTIINMGSEIKNPEKNIARGIAISLSISAVLYIILQSTFITSMPQSMLQHSG
                     WNGINFNSPFADLAILLGINWLAILLYIEAFVSPFGTGVSFVAVTGRVLRAMEKNGHI
                     PKFLGKMNEKYHIPRVAIIFNAIISMIMVTLFRDWGTLAAVISTATLVAYLTGPTTVI
                     ALRKMGPTMTRPFRAKILKVMSPLSFVLASLAIYWAMWPTTAEVILIIILGLPIYFFY
                     EYRMNWRNTKKQIGGSLWIIVYLIVLSILSFIGSKEFKGLNMIHYPFDFIVIIIVALI
                     FYYIGTTSSFESVYFRRATRINTKMRESLNNESKSSH"
     gene            8288..8887
                     /locus_tag="ACGEXS_000663"
     CDS             8288..8887
                     /locus_tag="ACGEXS_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family protein"
                     /protein_id="HFF8011701.1"
                     /translation="MSEKQSNEQVEAFLNKESQWQEEYKYLRALIFNETELEEAYKWM
                     HPCYTLNNKNVVLIHGFKNYVALLFHKGAILEDKYHTLIQQTEKVQAARQLRFENLTE
                     IQARTEEIKYYLAEAIKAEKVGKKVEMKKTEEYVIPKELEAKFEEMPQLESSFYKLTP
                     GRQHQYIYHIGQAKRSETRQKRVEKYINQILEGKGMHDK"
     gene            8891..9031
                     /locus_tag="ACGEXS_000664"
     CDS             8891..9031
                     /locus_tag="ACGEXS_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011702.1"
                     /translation="MSKAYRLYNNIQDDTKQPMAHAVGCFFRCVCHGQHFDVGIPLQA
                     WE"
     gene            9111..9230
                     /locus_tag="ACGEXS_000665"
     CDS             9111..9230
                     /locus_tag="ACGEXS_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011703.1"
                     /translation="MGGCPSVTMDACALLQKFDFCNNISHFRHFFAIKQPIER"
     gene            9557..10783
                     /locus_tag="ACGEXS_000666"
     CDS             9557..10783
                     /locus_tag="ACGEXS_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8011704.1"
                     /translation="MLSIKHLTKIYSGNKKAVDDISLDIQSGEFIAFIGTSGSGKTTA
                     LRMINRMIEATEGQIEIDGKDVRSMNPVELRRNIGYVIQQIGLMPHMTIKENIVLVPK
                     LLKWTKEEKDKRAKELIKLVDLPESFLERYPAELSGGQQQRIGVVRALAAEQDIILMD
                     EPFGALDPITRDTLQDLVKTLQRKLGKTFIFVTHDMDEAIKLADKICIMSEGKVVQFD
                     TPDNILRHPANDFVRDFIGQNRLIQDRPNDKTVEGVMIKPITIQAEATLNDAVHIMRQ
                     KRVDTIFVVDSNNHLLGFLDIEDINQGIRGHKSLRDTMQQHIYTVQIDSKLQDSVRTI
                     LKRNVRNVPVVDDQQRLVGLITRANVVDIVYDTIWGDSEDTVQTEHVGEDTASSKVHE
                     QHTTNVKVRDIGDDKS"
     gene            10780..11415
                     /locus_tag="ACGEXS_000667"
     CDS             10780..11415
                     /locus_tag="ACGEXS_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8011705.1"
                     /translation="MIEFLHEHGGQLMSKTLEHFYISIVALLLAIIVAVPIGILLSKT
                     KRTANIVLTVAGVLQTIPTLAVLAIMIPIFGVGKTPAIVALFIYVLLPILNNTVLGVQ
                     NIDSNIKEAGKSMGMTQFQLMKDVELPLALPLIIGGIRLSSVYVISWATLASYVGAGG
                     LGDFIFNGLNLYDPLMIVTATVLVTALALGVDALLALVEKWVVPKGLKVSG"
     gene            11432..12373
                     /locus_tag="ACGEXS_000668"
     CDS             11432..12373
                     /locus_tag="ACGEXS_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFF8011706.1"
                     /translation="MKKIKYILVVFVLSLTVLSGCSLPGLGSKSTKNDVKITALSTSE
                     SQIISHMLRLLIEHDTHGKIKPTLVNNLGSSTIQHNALINGDANISGVRYNGTDLTGA
                     LKEAPIKNPKKAMIATQQGFKKKFDQTFFDSYGFANTYAFMVTKETAKKYHLETVSDL
                     AKHSKDLRLGMDSSWMNRKGDGYEGFKKEYGFDFGTVRPMQIGLVYDALNSEKLDVAL
                     GYSTDGRIAAYDLKVLKDDKQFFPPYAASAVATNELLRQHPELKTTINKLTGKISTSE
                     MQRLNYEADGKGKEPAVVAEEFLKKHHYFDKQKGGHK"
     gene            12373..13068
                     /locus_tag="ACGEXS_000669"
     CDS             12373..13068
                     /locus_tag="ACGEXS_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8011707.1"
                     /translation="MEGNLLQQLFNYYATNFGYLWDLFFKHLLMSVYGVLFAALIGIP
                     LGILLARYTKLSGFVITIANIIQTVPVIAMLAILMLVMGLGSETVVLTVFLYALLPII
                     KNTYTGIASVDANIKDAGKGMGMTRNQVLRMIELPLSVSVIIGGIRIALVVAIGVVAV
                     GSFIGAPTLGDIVIRGTNATDGTTFILAGAIPIAIIAIVIDVLLRFLEKRLDPTTRHR
                     KNQSNHRPQSINM"
     gene            13362..14762
                     /locus_tag="ACGEXS_000670"
     CDS             13362..14762
                     /locus_tag="ACGEXS_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8011708.1"
                     /translation="MDTSKQFRGDNRLLLGIVLGVITFWLFAQSLVNLVVPLQSTYSS
                     DVGTINIAVSLSALFAGLFIVGAGDVADKFGRVKITYVGLILNVVGSLLIIITPLPAF
                     LIIGRIIQGLSAACIMPSTLAIINEYYIGTRRQRALSYWSIGSWGGSGICTLFGGLMA
                     TYIGWRSIFVVSILLTLLAMYLIKHAPETKAEPIKGMKAEAKKFDVIGLVILVVTMLS
                     LNVIITQTSHFGLVSPLILGLIVVFICSLVGFVYYENKIKHPLVDFSIFKNRGYSGAT
                     ISNFLLNGVAGGALIVINTYYQQQLGFNSSQTGYISLTYLITVLSMIRVGEKILSQHG
                     PKRPLLLGSGFTVIGLILLSLTFLPEVWYIISSIVGYLLFGTGLGLYATPSTDTAVAS
                     APDDKSGVASGVYKMASSLGNAFGVAVSGTVYTVLAANLNLNLGGFTGMMFNALLAIV
                     AFLVILLLVPKNQTNL"
     gene            14988..15923
                     /locus_tag="ACGEXS_000671"
     CDS             14988..15923
                     /locus_tag="ACGEXS_000671"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFF8011709.1"
                     /translation="MKIAIAGSGALGSGFGAKLFQAGYDVTLIDGYTSHVEAVKQHGL
                     NITINGEAFELNIPMYHFNDQPDESIYDVVFLFPKSMQLKEVMEDMKPHIDNETIVVC
                     TMNGLKHEEVIAQYVAQSQIVRGVTTWTAGLESPGHSHLLGSGPVEIGELVDEGKENV
                     IKVADLLNEAELNGVISKDLYQSIWKKICVNGTANALSTVLECNMASLNESSYAKCLI
                     YKLTQEIVHVATIDNVHLNVDEVFEYLVDLNEKVGAHYPSMYQDLIVNNRKTEIDYIN
                     GAVATLGKQRHIEAPVNRFITDLIHTKESQRHAQD"
     gene            16245..17096
                     /locus_tag="ACGEXS_000672"
     CDS             16245..17096
                     /locus_tag="ACGEXS_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFF8011710.1"
                     /translation="MSKIFVTGATGLIGIKLVQRLKEEGHEVAGFTTSENGQQKLAAV
                     NVKAYIGDILKADTIDQALADFKPEIIINQITDLKNVDMAANTKVRIEGSKNLIDAAK
                     KHDVKKVIAQSIAFMYEPGEGLANEETSLDFNSTGDRKVTVDGVVGLEEETARMDEYV
                     VLRFGWLYGPGTWYGKDGMIYNQFMDGQVTLSDGVTSFVHLDDAVETSIQAIHFENGI
                     YNVADDAPVKGSEFAEWYKEQLGVEPNIDIQPAQPFERGVSNEKFKAQGGTLIYQTWK
                     DGMNPIK"
     gene            complement(17188..18684)
                     /locus_tag="ACGEXS_000673"
     CDS             complement(17188..18684)
                     /locus_tag="ACGEXS_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFF8011711.1"
                     /translation="MTTKKLYFLSISIIILVAISIAIYITLNSNTKTRLTNDSQQQID
                     TIIEHDLQKGHIPGASILIVKNGKVFLNKGYGYQDVDKKVKASPTTKYEIASNTKAFT
                     GLAILKLAQEGRLNLNDAVSKHVPHFKMNYNGQNETITIKQLLAQTSGIPSDITSEDS
                     VTSKNNRLNDVTRAIMGDELHHKPGEEFEYSNMNYDLLGLIIQNVTKQSYTKYITNSW
                     LKPLHMTHTSFKQTNYKSKHDAIGYELQGSTPVVSKPEFNLWDTPSAYMMTSTEDLEH
                     WIKFQLNPPDKYKSLVQQSHKNLSSTIGEPNANAYASGWFTNNDEHLVFHSGTLDNFS
                     SFILLNPKQNYGIVVLANLNSEYVPKLVEHLNTQIVNHKRYSTVASMLNQYKDQFNIV
                     TVLMTTLILLAFIFSAYRAWQMRHGQILLRRSKRIAVLSWLSLCICIALALILYALPY
                     LILGSNNWSFVLTWLPIEIKLALITTLIALFSTLIVILLFLHTKITKT"
     gene            18916..20745
                     /locus_tag="ACGEXS_000674"
     CDS             18916..20745
                     /locus_tag="ACGEXS_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFF8011712.1"
                     /translation="MFNQFKRLIIGQPKKNRELKDEKISKFKGLAILSSDALSSVAYG
                     PEQILITLSVVGAVATWYTLPIAGAVLILLAALIMSYRQIIYAYPKGGGAYMVSKTNL
                     GEKWGLLAGGSLLVDYILTVAVSISSGADAFVAAFPSLYGHKVLIACLLVLFILILNL
                     RGLTESATVLSYPVYLFIIGLVILIFIGTFRVATGDIQPHMHASVGTAVPGVTLFLLL
                     KAFSSGASSLTGVEAISNAVTNFREPSAKNAVKTLIAMGSILAFLLVGIVGLAYVYGI
                     LPQTETTVLSQLAMQIFGDNAAFYFVQATTVMILVLAANTGFTAFPMLAASMSKDKYM
                     PRMFTVRGDRLGYSNSIIILGVLAIILIIVFDGMTEDLIPLYAVGVFIPFTLAQFGMV
                     IKWIHERPKNWLSKLSVNLLGGIVTFIVFMILLITKFSQVWPILIFLPFVVIFFLKIN
                     KHYRDIAEQLRSDIDVLNVDVVDRNLAIVPITSITTAVDKSIYYAQMLANNDVIAVHV
                     SFGDEDEKAFQEKWKRHFPDVRLVILHSEYRSIIRPISRFIDKINRKANDQNYMITVV
                     IPEFITKKRWHNLLHNQTSLRMKLYLIYQKNVNVCTIPFKLKK"
     gene            complement(20885..22963)
                     /locus_tag="ACGEXS_000675"
     CDS             complement(20885..22963)
                     /locus_tag="ACGEXS_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFF8011713.1"
                     /translation="MALLEAFLIFIFAVIISSVINNRFPQIPTAFIQIALGVVIFIIP
                     IQVDFQFNSEVFMFAVIAPLLFVEGTHVSRTKLLEYRKPILLMSMALVFATVVGVGYF
                     IHWIWPALPMPAAFAIAAILCPTDAVAVSAITRGKLLPKGSMTILEGESLLNDAAGII
                     SFKIAVTALVTGTFSLFQAVEQFIISTILGVLIGAIIGFVVVRIRIDLTANKGLKDNN
                     TLTFIQLLTPFVVYFLAEEVHASGIIAVVIAGLIHGLERDRLIRAQTELQMNYHQIWN
                     TFSYALNGFVFVVLGFMIPTVVIDIFQTEPDNFSFLIVITILIAIAIYACRFVWVYFW
                     YKDFYFPKNIQSYLDEEHDSHETPPSRVRYAFIMTMCGIHGTISLSMALTLPFIITKG
                     QAFEYRNDLLFIASFMVLISLILAQIVLPLITPSAEDTTFKGMTYQSAKIFIVQKVIQ
                     HFKNESKKDKNDTNYRPVLNQYYGELLFLLNSEPDNQNTKELKRLEDIAKVIETSTLE
                     RLIDKGKATYQDINNYRNIVELTETHRTASFVEKFANFFKMLWLRLKAMKHYKALNKE
                     SKKQEFENSFKDVQKIMRIVNHNIILRLKEEQNSTNVLEVSLVINHYYDMSRSLKWRA
                     QRRKERQENSNQIIPQAMFHNHKLEALYLQRHLLDELIRKNKINNIVAAQIRENINYN
                     EIVLSLQSKH"
     gene            complement(23101..24510)
                     /locus_tag="ACGEXS_000676"
     CDS             complement(23101..24510)
                     /locus_tag="ACGEXS_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFF8011714.1"
                     /translation="MARKLHRELNNRHIQLIAIGGAIGTGLFLGSGQTISLTGPSLLF
                     TYMIIGVVLFAFMRALGELLLSNTRFNSFVDIANEYLGPFGGFVIGWTYWLCWIVSSM
                     SDLTAMGQYFAFWYPQVPNWITVLFIVLILISFNLLGARLFGELEFWFSIIKVVTIIA
                     MVIVGLVLIFFSFKTHYGHASFTNLISHGGMFPGGTFGFLMSFQIAVYSFIGIELIGV
                     TAGETKDPEKTLPKAINNVPIRILLFYIGGLLVIMSVIPWNDIDPNSSPFVKLFTLIG
                     VPFAAGVVNFVVLTAAASATNSGIYSNSRILFGLSQQGLGPKVLNKTNSHGVPYLSML
                     VSSIALLIAALLNYIFPNAIQLFIYVTTLSTVLFLVVWAMIIVAYLMYLKKHPEAHKN
                     SKFKLIGGKPIAYIILAFFFFVFILLFFSDETRAAIYISPFWFIFLFFFYKKYKTNAE
                     KLAYEQRQNDSGHFRYDNQ"
BASE COUNT         8162 a         3632 c         4693 g         8088 t
ORIGIN      
        1 cacaaaattt taaaaatatt taagccagaa aatgataaag cggtagggaa cgtttttctg
       61 aaagttagtg atacaatagt tttaagttga aatacaggag gatgaataac atgaatcagt
      121 cagtcaaatt acttaaacat ttaacagatg taaacggcat tgctggttat gaaatgcaag
      181 ttaaagaagc aatgcgtaac tatatagagc ctgtcagtga tcaaattatt gaagataact
      241 tgggtggcat ttttggaaag aaaaatgctg agaatggtca atactcaatt atgatttctg
      301 gtcatatgga tgaagttggt tttatggtaa caaagattga taaacatggt tttatttcat
      361 ttacgccagt tggtggatgg tggaatcaag tcatgctatc tcaaaaagta acgattacaa
      421 cagattcggg caaagaaatt agaggtatca tcggttctaa accgccacat gtcttaacgc
      481 ctgaagaacg taaaaagcca atggaaatca aaaatatgtt tatagatatt ggtgttagta
      541 gcaaggaaga agctgaagaa gctggcgttg aagtaggcaa tatggttacg ccatatagtg
      601 aatttgaagt gcttgcaaat gataaatatt taactgcgaa agcatttgat aatcgctatg
      661 gctgtgcatt agctattgag gtattaaaac gtttaaaaga tgaaaatatt ggcattaact
      721 tatacagtgg tgccacagtg caagaagaag ttggtttgcg tggtgcgaaa gtggcagcga
      781 atacgattaa accagacttg gcgatagctg tcgatgtagg tattgcttat gataccccag
      841 gtatgtcagg tcaaacgagc gatagtaaac taggcggtgg tccagttgtc attatgatgg
      901 atgctacaag tattgctcac caaggtttgc gaaagcatat taaagatgta gctaaggaac
      961 ataacatcga agtacaatgg gatacgacac caggtggagg tacagatgcg ggaagtattc
     1021 atgtcgcaaa tgaaggtatt ccaacgatga caatcggtgt tacgctgcga tacatgcatt
     1081 ctaatgtttc agtgctcaat gtagatgatt atgaaaattc tatccgtctt gttactgaaa
     1141 ttgtccgttc attgaatgat gaaagttata aaaatatcat gtggtaatca aatccataaa
     1201 taataaagaa tccttttaat atggtaggtt gttaaacaat tgtctaattt taattcttag
     1261 tcattagaca gtatccatgt taataggatt ttttgttttt aatttaaatg ctgaaaatca
     1321 attatgccta aattttgata ttacaagaaa atgatttttt cttaaatgta attgcactaa
     1381 aaaccaaaaa aacgggaata atatacctga tatattacat gaggagcggt gcaaatgttg
     1441 ttagaaatta aagatttagt gtataaagcg agcgatagaa tcatactaga tcatatcagt
     1501 ctaaaagtag ataaaggcga gagtattgcc attataggtc catcaggtag tggtaaaagt
     1561 acatttcaaa agcaaatatg taatttgttt agtccaacta gtggagaact ttattttaaa
     1621 ggtaaaccct ataatgatta tgacccggaa gaattgcgtc aacgaatcag ttatttgatg
     1681 cagcaaagtg acttgtttgg tgaaacgatt gaagataaca tgatattccc atcacttgca
     1741 cgtaatgata aatttgatag aaaacgtgca aagcaattaa ttaaagatgt cggtttggga
     1801 cattatcaat taagttcgga agtggaaaat atgtcgggtg gtgagcggca aagaattgct
     1861 atagcgcgcc aactgatgta tacaccggat attcttttat tagatgaatc gaccagtgca
     1921 ttagacgtta ataataaaga aaagatagaa aatatcattt ttaaattagc agatcaaggc
     1981 gtggcaatta tgtggattac ccacagcgat gaccaaagta tgcgacactt tcaaaagcgt
     2041 ataacaattg ttgatggtca aatttctaat acagaggagt tgaatcaaca tgagtaatac
     2101 ggcacttgga ttgacggcat tgcttttagt cattccgatt atcatttcat ataaagaagg
     2161 tttacatatt attaaagatt taattgttgc gacattacga gcagttgtgc aattaatcat
     2221 tttgggattt ttgctgcatt atatttttaa aataaacgat aaatggctgc ttattttatg
     2281 tgtattggtc attattatta atgcatcatg gaatacaatt agtcgagcat caccagtgat
     2341 gcatcatgtg ttttggatat catttctagc tatcttcatt ggaacggcat taccgcttgc
     2401 aggtactatt gcgacagggg ccattcaatt taccgcaaat gaagttatac ctatcggcgg
     2461 catgcttgca aataatggct tgattgcaat taatttagct taccagaatt tagatcgtgc
     2521 attcgtacaa gatggtacta atattgaatc taaattatca cttgcagcta cacctaaatt
     2581 ggcttctaaa ggtgcaatac gtgaaagtat tcgtttagct atagtgccaa ctattgattc
     2641 ggttaaaaca tatgggcttg tgtcgattcc tggtatgatg acaggcttaa ttattggtgg
     2701 cgtaccacct ttacaagcga ttaaatttca attgttagtc gtgtttattc atacaactgc
     2761 gaccattatg tctgctttga ttgcgacata tttaagctat ggtcaatttt tcaatgcaag
     2821 acatcaatta gtagcacgaa atactgatgt taagagtgaa tcatgataga ttttactgca
     2881 tcagatttag gcattagttt taattggaaa tgaagtgacg cgcacatata gtatcgctat
     2941 tcattagcgc agcgaaaata ttcataaagg cacgcatact ttgtagtcag ttatctgttc
     3001 tgacatataa agcgtgcgtg cttttttgga gttattgttg aaactgaagt aattatacat
     3061 aattattaaa tgacatactt gtgttaattt ttcaaatact gaaaaacaat ttcaataatt
     3121 ttccaattaa gcacagaaaa ttaaagcaaa atattatata atagaacggt tatatataaa
     3181 attgtgttgc acacattttt taataaatcg ttattctaag ggaaatgaat atcggaaatt
     3241 ttgtttgaaa ggagttttaa attgtcaatc atgcgactat ttacattcat tttaagtatt
     3301 tttatcgtag gaatggttga aatgatggtt gcaggaatta tgaacttgat gagtcaggac
     3361 ttacatgtat cagaagctgt cgttggtcaa ttagtgacaa tgtacgcttt aacatttgcg
     3421 atatgtggac ctattctggt taaattaacg aaccgttttt catcaaggcc tgtattatta
     3481 tggacattac ttatatttat cattggtaat ggcattattg ctgtagcgcc aaatttttca
     3541 atattagtag ttggtagaat tatctcatct gcagcagcag cactaattat cgtaaaagta
     3601 ttagctatta cagcgatgtt atcagcacct aaaaatcgtg gtaaaatgat tggacttgtc
     3661 tatacagggt ttagtggtgc taatgttttt ggtgtaccaa ttggaacggt tatcggcgat
     3721 ttagtaggtt ggcgctatac atttctattc ttaattattg tgagtattat tgttggcttc
     3781 ttgatgatga tctatttacc gaaggatcag gaaatacaac gaggccctgt gaatcatgag
     3841 acaccatctc atgaaaatca tgttacttcg aaaatattaa gacctgctga agtagcaaaa
     3901 tatttaatta ttacgttttt agtattgatt gcaaactcag tgacattcgt ctttataaat
     3961 ccacttattt tatctaatgg tcatgatatg tcattcgttt cattagcact tctagtaaat
     4021 ggaatcgctg gcgttattgg aacatcatta ggtggtatat tctccgataa aattacaagt
     4081 aagcgttggt taatgatttc tgtttctatt tttatcgtca tgatgttact tatgaattta
     4141 atcttacctg gttcaggtct attgttagca ggactattta tttggaatat catgcaatgg
     4201 agtactaatc cagcagtgca aagcggtgtg attcaacatg ttgaaggcga cacaagccaa
     4261 gtaatgagtt ggaacatgtc tagtttaaac gctggtattg gtgttggagg cattattgga
     4321 ggcttggtca tgacacatgt ttctgttcaa gctatcacat atacgagtgc catcattggc
     4381 gcattaggat taatcgttgt tttcacattg aaaaataatc attatgctaa aacatttaaa
     4441 tcatcataat tctcatatga aaagcacgcc tgctatcaaa ttcaggtgtg cttttttaga
     4501 tgcgataacg ttattgatat gtgcgataat agcgacgttc attatgatac atcggccaag
     4561 gcattttacc gcttttagca aaattagcta aatcattttg catttgtcga cttaaaaatt
     4621 taaggtgagc agttgttgga tattgatgtg ctgctaatat ttgtaagttt ccgaaccaaa
     4681 ataccatatc tagaatatgg taagcactgc gatagtgtgc gctagaagta tcatgccatg
     4741 caaattcagc aagccatagt tgtgcgtttg aatcttgtat attgagttgt tgtaaaaatt
     4801 gcttcatcgg ctgtttgaaa taacaatgtg taatcgctaa agattgttgc ttcgccgtct
     4861 gaacatcttc gtattttaaa ggaatatcat ttaattccat aatgtcgata aagcgttgcg
     4921 gcgataattt tttctgttca ctagtaatat aaatatcgcc ttcatctttt gtataacatg
     4981 caaaaattgg tttcgttgtt ggataattat tttgtatata atctgtttta ataggcgcat
     5041 atattaaatc aagcccttta gaaggtcctc gagattgttt tagcttcgcc atcagcataa
     5101 gaatatcatt tgtcgataat gatgtaacat catctgtatc taaataatcg agcatcattt
     5161 tttggaaatg ttgtgcttta ttgcgtgcac tctcaagggt gtctaatcgt agtgcgccac
     5221 ttagtagaac gactttatgg aagtatggct caatgtcagg tattttaagt aaagtcaaaa
     5281 tgctcatact gcctgcagac tgacccatta aagtaatgtt attagcgtcg ccaccgaagg
     5341 attcaataaa ttgatgcacc cattttatga cattgatttg atctgaaagg ccattattgg
     5401 aatgaaaatc tttattaaaa tatgaccagt ctaaatatcc taatgcgcct aaacgataat
     5461 tgcatgtaat aacgataatg tcgttatttt gtactaaatg tgccggttga tagagttcgg
     5521 ctgtaccatg accattttca aaactaccac cataaaaata aatgatgaca ggtttcttcg
     5581 tctgatcatt atgttgtttc caaatattta gatataaaca gtcttcatgt tcagtaaaag
     5641 ttgtagattg tgaggagaaa aaatcttcta atttgttgtc tggttgcggt gggatgggtt
     5701 gtatttcagt tgcatcaatt ggctctgacc attgtgtttt taacgtagaa tgtttaaagc
     5761 gattgtcatg aactggtggt tctgcataag gaatgcctaa gaagatatct aaaccatctt
     5821 gtgtaatacc atgaatttga ccacctgtag tattaatctt catatttatt aacctccttt
     5881 atcatcccca atttaatcat accacggtct atagatgata ggtgacaagt ataacaagac
     5941 aattttgtga ctgaatcaga cgagcttgat atctgtttta actcaaacca ttttcagcat
     6001 tatatgatgt acttctacgg gagtgggaca gaaatgataa agagccacaa atgatttatt
     6061 atgtagtggt tcttatacat tagccacagc taatgtgtac ttaaaaatat gaatacatga
     6121 gtaaaactca tgcataagaa atactaattt ctatagaaaa agtatttctt tatcgtcgtc
     6181 ccaccccaac ttgcattgcc tgtagaattt cttttcgaaa ttctctgtgt tggggcccct
     6241 gactagaatc gaaaaaagct tgttacaagc gcattttcgt tcagtcaact actgccaata
     6301 taacttcgta gagcatagaa cattgattta tgtcccagcc tgatcatcat cttgttatgt
     6361 tagttattta taaccgattt tcaaaataaa aaaccaacca agatgttgaa atcaatggtt
     6421 ggttaatgtg tgtgtgttga aatcatatat ggcactgtat tttaaaatat ttaatgagat
     6481 gactttgatt cattatttag tgactcacgc atcttcgtat tgattcgtgt tgcgcgacgg
     6541 aaatagacgc tttcaaatga actcgttgta ccgatgtaat agaagataag tgccacaata
     6601 ataataacga taaagtcaaa tggatagtga atcatattta agcctttaaa ttctttgctt
     6661 cctataaatg acagtattga tagcacgatt aaatatacaa taatccataa gctaccacca
     6721 atttgtttct ttgtattacg ccaattcata cgatattcat agaagaagta gattggtaat
     6781 ccaagtataa tgattaaaat aacttcagcc gttgttggcc acattgccca atatatagct
     6841 aatgaagcta atacaaatga taatggtgac attactttta aaatttttgc tctaaatgga
     6901 cgagtcattg ttggtcccat ttttcttaat gcaatcactg tcgttgggcc agttaaatag
     6961 gctactaaag ttgcagtaga aataactgct gctagcgtac cccaatctct aaataatgta
     7021 accataatca tactaatgat ggcattaaag atgattgcta cacgtgggat atgatatttt
     7081 tcattcatct tcccaagaaa tttagggata tgtccatttt tctccattgc tcgtaaaact
     7141 cgacctgtaa cggcgacaaa tgacacgcca gtaccgaatg gtgatacaaa agcttcaatg
     7201 tatagtaaaa ttgcgagcca attaattcct aataagatag ctaaatcagc aaatggtgaa
     7261 ttgaagttga tgccattcca tccactatgt tgtaacattg attgaggcat agaagtgata
     7321 aacgtacttt gtaaaatgat gtacaacact gcactgattg acagtgagat agcgatgcct
     7381 cttgcgatat ttttttcagg atttttaatt tctgaaccca tattaataat tgtctggaat
     7441 gaattgaatg aaaaaataat ccctgatgct gttgttgcag caaaaatcgg tgcacttccg
     7501 taaggcataa atgtgcttgc cgaatggccg taatttgaag tgtcgaatcc tgatagcatc
     7561 aacatgatga tggttaacat gggtacgcct aatttaaata cagaaattaa actcgtaaat
     7621 gatgttaaaa gttttacgga ccaatagttt aataatgaaa aaataacaat gatgagatat
     7681 acagctagca atccgtatgt gctaatagag ccattttcca ttaaatatct cattggtttc
     7741 gcccaatgcc acggccaaga actcatatat tgaacagctg acacagcttc gataggtatt
     7801 attgtcacca aagacaccca attcgcccaa gcagcaataa agcctaataa tgagccatgt
     7861 gtatactggg catagttact catgccacct gattgaggaa acattgtgcc gatttcaatg
     7921 tagttatagg caatggttcc aatgactagg aatccaagaa cccatgatat gattgctgct
     7981 ggtccagcta ttgatgatgc ttcccacgca ccaaatagcc aaccagatcc tattaaagag
     8041 cctaacccta ataagactaa ttgggataga ttgattttgt cgttgtgcaa ttgcttgtct
     8101 ttattcataa atactccttc gttattgcca aaaatacaaa aaataatatg cgacaattat
     8161 acgcttatta taataatagt caaatgcatt ttttgtaaga acgcaaaagc atttgtaaaa
     8221 ttagtttttt ataaaattag gatatataat acaatgtgta ttgaaaaaca aaatggaagt
     8281 gatgactatg agtgagaaac aatcaaatga acaagttgaa gcatttttaa ataaagagag
     8341 tcaatggcaa gaagaatata aatatttgag agcgttaatc tttaatgaaa cagaattaga
     8401 ggaagcgtat aaatggatgc atccttgtta cacgttgaat aataaaaatg tagtacttat
     8461 ccatggcttc aaaaattatg ttgcactatt atttcataaa ggtgccattt tggaggataa
     8521 atatcataca ctcattcaac agactgaaaa ggtgcaagca gctcgtcagt tacgatttga
     8581 aaatttaaca gagattcaag cacgtaccga agaaattaaa tattatctag ccgaagcaat
     8641 taaagctgaa aaagttggta aaaaagttga aatgaagaaa acagaggaat atgttattcc
     8701 taaagagtta gaagctaagt tcgaagaaat gccacagtta gagtcttcat tttataaatt
     8761 aacgccaggc agacaacatc aatatatata tcatattgga caagctaaac gcagtgaaac
     8821 aagacaaaag cgtgttgaaa agtatattaa ccaaatacta gaaggtaaag ggatgcatga
     8881 taagtaatta atgagtaaag cataccggtt atacaacaac atacaagatg acacgaaaca
     8941 accaatggct catgctgttg gttgtttttt taggtgtgtc tgtcatgggc aacactttga
     9001 cgttggaatt ccgttacagg cttgggagta gaaaatgtta gcaaaaggca agggtgtcta
     9061 caatgaatga tgaagatatt aaaatataag gatgactttg tgagtggcgg atgggcggtt
     9121 gtccgtctgt aacaatggat gcgtgtgcat tattacaaaa attcgacttt tgtaataata
     9181 tttcacattt tcgacacttt tttgctataa aacaaccaat tgagcgataa taaattcgct
     9241 tttaaaaaat atgagttatc tatttagttg ccaaagataa aataataatg tttaataaca
     9301 tcatatagag tatgttagtt ttaaatgtcg aatatacgaa tgtgcaaaca aagtaatcgg
     9361 tagaaattca acatacatag cgccgtttac tgttaagtat tcacattaca gatgaaaaat
     9421 ataaaattct acataatcaa gaccatgatg tgtacttgtt taacttatga ctctatttgt
     9481 ttaacaattg cgataatggt ctttttattt tatgcgtatc attcgtcata ttttttatga
     9541 ggaaggagaa atgattatgt taagtattaa gcatttaacg aaaatttatt ctggtaataa
     9601 aaaggcagta gatgacatct ctttagatat tcaatctggg gaatttatcg catttattgg
     9661 aaccagtgga agtggcaaaa cgactgcttt aagaatgata aaccgtatga ttgaagcgac
     9721 agaaggacaa attgaaattg atggtaaaga tgttcggagt atgaatcctg tcgaattgcg
     9781 tagaaatatt ggctatgtta ttcaacaaat tggcttaatg cctcatatga cgattaaaga
     9841 gaatattgtg ttggtaccca aattgttgaa atggactaaa gaggaaaagg ataaacgtgc
     9901 aaaggaatta attaaacttg tggatttacc ggagtcattt ttagagcgtt atccagcaga
     9961 actatcaggt gggcaacaac aacgtatcgg tgttgtaaga gcacttgcgg ccgaacaaga
    10021 tattatttta atggatgaac cttttggtgc attggatcct attacgagag atacgttaca
    10081 agatttagtt aaaacgttac aacgaaaatt aggcaagacg tttatctttg taacacatga
    10141 tatggatgaa gcgattaaat tagcagacaa aatttgtatt atgtcagaag gtaaggtggt
    10201 gcaatttgat acgccagaca atattttaag acatcccgca aatgattttg tacgtgattt
    10261 tataggacaa aatagactga ttcaagaccg tcccaatgac aagactgtag aaggtgtaat
    10321 gattaaacca atcacgatac aagcagaagc aacactgaat gacgccgttc atattatgag
    10381 acaaaaacgt gttgatacta tttttgtagt agatagtaat aaccatttac taggtttctt
    10441 agacattgaa gatataaatc agggtatacg tggacacaaa agtttacgag acaccatgca
    10501 acaacatatt tataccgttc aaattgattc taaattacaa gattctgtac gtacgatttt
    10561 aaaaagaaac gttaggaatg tacctgtcgt agatgatcaa cagcgtttag taggactgat
    10621 tacgcgtgcc aatgttgttg atattgtata tgacacgatt tggggcgata gtgaggatac
    10681 agtgcaaaca gaacatgtgg gggaagacac tgcgtcctca aaagtgcatg agcaacacac
    10741 tactaatgtc aaagtacgtg acataggaga tgataaatca tgattgagtt cctacatgaa
    10801 catggtggac agttgatgtc gaaaacactg gaacatttct atatttctat agtggcatta
    10861 ttacttgcca tcattgttgc agtacctata ggcattttat tatcaaaaac aaagcgaact
    10921 gccaatattg tattaactgt ggcaggtgtc ttacaaacta ttccaacact agctgtactt
    10981 gctattatga taccgatttt tggtgttggt aaaacgcctg caattgtagc gctatttatt
    11041 tatgtattat tacctatttt aaataacacg gtactcggtg ttcaaaatat tgatagcaac
    11101 attaaagaag ctggaaaaag tatgggaatg acacaatttc aattgatgaa ggatgttgaa
    11161 ttgccgttag cattgccgct tatcattggt ggcattcgtt tgtcatctgt gtatgtaatt
    11221 agttgggcta cacttgcaag ttatgtaggt gcgggtggat taggtgattt cattttcaat
    11281 ggtttaaatt tatatgatcc actgatgatt gtaactgcaa cggtactcgt tactgcacta
    11341 gcattaggtg ttgatgcctt attagcttta gttgaaaaat gggtagttcc caaaggctta
    11401 aaagtatctg gataattagg aggctaagat aatgaagaaa attaaatata tacttgtcgt
    11461 gtttgtctta tcgcttaccg tattatctgg atgtagtttg cccggactag gtagtaagag
    11521 cacgaaaaat gatgtcaaaa ttacagcatt atcaacaagc gaatcgcaaa ttatttcaca
    11581 tatgttacgg ttgttaatag agcatgatac acacggtaag ataaagccaa cattagtaaa
    11641 taatttaggg tcaagtacga ttcaacataa tgccttaatt aatggggatg ctaatatatc
    11701 aggtgttaga tataatggca cagatttaac gggagctttg aaggaagcac caattaaaaa
    11761 tcctaagaaa gcaatgatag caacacaaca aggatttaaa aagaaatttg atcaaacgtt
    11821 ttttgattcg tatggttttg cgaatacgta tgcattcatg gtaacgaagg aaaccgctaa
    11881 aaaatatcat ttagagacag tttcagattt agcaaagcat agtaaagatt tacgtttagg
    11941 tatggatagt tcatggatga atcgtaaagg cgatggctat gaaggattta aaaaagagta
    12001 tggttttgac tttggtacag tgagaccaat gcaaataggt ctagtctacg acgcattaaa
    12061 ctcagagaag ttagacgttg cattaggtta ttctacagat ggtcgaattg cggcgtatga
    12121 tttgaaagta cttaaagatg ataaacaatt tttcccacct tatgctgcga gtgctgttgc
    12181 aacaaatgaa ttattacggc aacacccaga acttaaaacg acgattaata agttgacagg
    12241 aaagatttcg acttcagaga tgcaacgctt gaattatgaa gcggatggta aaggtaaaga
    12301 acctgctgtc gtcgcagaag aatttttgaa aaaacaccac tattttgata aacagaaagg
    12361 tggtcataag taatggaagg taatttatta cagcaattat tcaattatta tgctacgaac
    12421 tttggttatc tatgggattt atttttcaaa cacttattaa tgtctgtcta tggtgtgctg
    12481 tttgcagctt taattggtat tccattggga atcttgcttg caagatacac aaaactttct
    12541 ggatttgtaa ttacaattgc aaatataatt caaacagttc cagtcattgc aatgttagct
    12601 attttaatgt tagtcatggg cttaggttca gaaacagtag ttttaacagt gtttttatat
    12661 gcgttacttc caattataaa aaacacttat actggtatag ctagtgttga tgcgaatatt
    12721 aaggatgctg gcaaaggtat gggaatgaca cgcaatcaag tgctacgaat gattgaatta
    12781 ccgttatctg tttcggttat tatcggtggc attcgtattg ccttggttgt tgcgataggt
    12841 gttgttgccg ttggatcatt tataggagca cctacgcttg gtgacattgt gattcgtggt
    12901 acaaatgcga cggatggcac aacgtttatt ttagcaggtg cgattccgat tgctatcatt
    12961 gcaatcgtca ttgatgtact attaagattt ttagaaaaac gattagaccc aacaacacga
    13021 catcgtaaaa atcaatctaa tcatcggccg caaagtatta atatgtaata gtagaagatg
    13081 tttataattt agcgatttcg tttcatgatt tataaaaaat gaggctactc aaggagctca
    13141 aataatcttt gagtagcctt tttataggtt gtgtttgtat gcgtttacac taaaatagca
    13201 attattatca tgaaagtttt tggataaaaa gcgttaatta ttgtaaaaat actaaaaaat
    13261 gagatgtttt atttataatt ttctgcaaat ttatgatatt gtttcttaat atatcatatt
    13321 aaaaatttgt ttttcttaaa cataggaggc ttatctaatt catggacaca tcaaaacaat
    13381 ttagaggtga caaccgattg cttttgggta tcgttttagg ggttattacc ttttggctat
    13441 tcgcgcagtc acttgttaat cttgttgtcc cattacaatc aacatatagt agtgacgttg
    13501 gaacgataaa tatcgctgtt agcttatctg ccttatttgc tggtttgttt atcgtaggtg
    13561 ctggtgatgt tgctgataaa tttggtcgcg tcaaaattac ttatgtagga ttgatattaa
    13621 atgttgtagg ttcattactc atcatcatta cacctttgcc agcattttta attataggta
    13681 gaataattca aggtttgtct gcagcatgta ttatgccatc aacacttgct attattaacg
    13741 aatattatat tggtacaaga agacaacgtg ccttaagcta ttggtctatt ggttcttggg
    13801 gtggtagtgg tatttgtacg ttgtttggtg gcttaatggc tacatatata ggttggcgtt
    13861 caatatttgt tgtttcaatt ctattaacat tattagcaat gtacttaatc aaacatgcac
    13921 ctgagactaa agcagaacca atcaaaggta tgaaagcaga agctaaaaag tttgacgtta
    13981 ttggtttagt cattttagta gtgacgatgt taagtttaaa tgtaatcatc acacagacgt
    14041 ctcattttgg tttagtttca ccgttaattc taggtttaat tgttgtgttt atctgttcat
    14101 tagttggttt tgtatattac gaaaataaaa taaagcatcc acttgttgat ttttcaattt
    14161 ttaaaaatag aggatacagt ggtgcaacta tttcaaactt cttattaaat ggtgtagcag
    14221 gtggtgcact tatcgttatt aacacgtatt atcaacaaca attaggattt aattcttcgc
    14281 aaacgggtta tatttcatta acgtatttaa taacagtgtt gtcaatgatt cgtgtaggtg
    14341 aaaagatttt atctcaacat ggtccgaagc gcccactatt actaggaagt ggctttacag
    14401 tgattgggtt aatcttattg tcgttaacat ttttaccaga agtgtggtat atcatatcta
    14461 gtatagttgg atatttattg tttggtactg gtttaggatt atatgctaca ccatcaactg
    14521 atacagcagt tgctagtgcg ccagatgata agtcgggtgt tgcttcaggt gtgtataaaa
    14581 tggcgtcatc attaggaaat gcatttggag tagcagtatc tggtacggtt tatactgtgt
    14641 tagcagctaa tttaaatttg aacttaggtg gtttcacagg tatgatgttt aatgccttgc
    14701 tagcaattgt tgcattttta gtcattttac tattagttcc taaaaatcaa acgaatttgt
    14761 aaaactgaaa tgaaagcaag ttattatgta gggattttaa aggaaatttt gtgaaagtaa
    14821 gtttatcata cacacttaat gttgcgtatt gacgtttaat gttaggtgtg ttcttttata
    14881 gacgataaaa gctgtgtgca tattaagcga atgattttca aattgacgct aatatgcgaa
    14941 agtagtattt ttaaaatgaa caacaacgat gaagaggggt ttataggatg aaaattgcaa
    15001 ttgctggatc gggtgcatta ggtagtggct ttggtgccaa actatttcaa gcaggatatg
    15061 atgtcacact tattgacgga tatacatctc atgttgaagc ggttaagcaa catggattaa
    15121 atataacgat taatggagag gcattcgagt taaacattcc gatgtatcat tttaatgatc
    15181 aaccggacga aagcatttac gatgttgtct ttctatttcc aaagtctatg caattaaaag
    15241 aagtgatgga agatatgaag ccacatattg ataatgaaac gatcgtcgta tgtacgatga
    15301 atggtctgaa gcatgaagaa gtcattgcgc agtatgttgc tcaatcacaa attgtcagag
    15361 gtgttacgac ttggacggca ggtcttgaaa gccctggaca cagtcattta cttggtagtg
    15421 gaccagttga aataggtgaa ctagtggatg aaggtaaaga aaatgttata aaagttgctg
    15481 atttacttaa cgaagcggaa ttgaatggtg tcattagtaa agatttatac caatcgattt
    15541 ggaaaaagat ttgtgttaat ggtacggcaa atgcattaag cacagtgttg gagtgtaata
    15601 tggcatcgct gaatgaaagt agttatgcga agtgtttgat ttataaatta acgcaagaaa
    15661 tagtgcatgt agcgacgatt gataatgttc atttaaatgt tgatgaagta tttgaatatt
    15721 tagttgattt aaatgaaaaa gttggtgcgc attatccatc catgtatcaa gatttaattg
    15781 ttaataatag aaaaactgaa attgattata ttaatggcgc agttgcaaca ttaggtaaac
    15841 aacgtcatat tgaagcgcca gtcaatcgct ttattactga tttaattcat actaaagaaa
    15901 gtcagcgaca cgcacaggat taattggtgt tttaggtatg attacaagga aatgacaatc
    15961 acgtgatatt acggtcatta ttaagattga aatgtaataa ataaagaaca gcagtaaggt
    16021 actttcaaat tgaaatgatc ttggtgctgt ttttcttgat tgatcttcgt cataattcag
    16081 atttgtcata ggtctacgac atactattag tatttactag acagttttta cgacgacact
    16141 ttgaaaaatt ttgaggcaaa tcatttggaa gtctcacgtg aattttgtaa actcatcaag
    16201 caagtaatta tattaaaaag acaaatagag aaaaggtgtt tataatgagt aaaatttttg
    16261 taactggtgc aacgggcctt attggcatta aattagttca aagactaaaa gaagaggggc
    16321 atgaggttgc tggttttact acatctgaga atggtcaaca aaagctagct gctgttaatg
    16381 taaaagcata tattggtgat atattaaaag ctgatactat tgatcaagcg ttagcagatt
    16441 ttaaaccaga aatcattatc aatcaaatta cggatttaaa aaatgttgat atggcagcaa
    16501 atacgaaagt acgtattgaa ggttctaaaa acctaattga tgcggcgaaa aagcatgacg
    16561 ttaagaaagt aattgcccaa agtattgcct ttatgtatga acctggcgaa ggattagcaa
    16621 atgaggaaac ttcacttgat tttaactcaa ctggcgatag aaaagtaacg gttgatggtg
    16681 tggttggttt agaagaagaa acggctcgta tggatgaata cgttgtttta cgttttggct
    16741 ggttatatgg cccaggtact tggtacggaa aagatggcat gatttataat caatttatgg
    16801 atggtcaagt gacactttca gatggcgtaa catcatttgt gcatcttgat gatgcagttg
    16861 aaacatctat tcaagctatt cattttgaaa atggtatcta taatgtagca gatgatgcac
    16921 ctgttaaagg ttctgaattt gcagaatggt ataaagaaca acttggtgtt gaaccaaata
    16981 ttgatattca acctgcgcaa ccatttgaac gtggcgtaag caatgagaag tttaaagcgc
    17041 aaggtggtac tctgatttat caaacttgga aagatggcat gaatccaatt aaataataat
    17101 ttatccgttt aatatacaaa gaataaagac ttggtcgaat cgtggatgat atattatcaa
    17161 acgcacggct cgaacaagtc ttttttatta tgtcttcgtt atctttgtat gaaggaataa
    17221 cagaattaca attaatgtac tgaataatgc aattaatgtt gtgattagtg ctaatttaat
    17281 ttctattggt agccaagtca gtacaaaaga ccaattattg ctaccgagaa tgagatatgg
    17341 taatgcatat aatatgagcg ctaaagcgat acatatacat aatgataacc aactcaatac
    17401 agcaatccgt tttgatctac gcaaaagaat ttgaccatgg cgcatttgcc aagcacgata
    17461 agctgagaat ataaatgcta ataaaataag tgttgtcatc aaaacggtaa caatattaaa
    17521 ttgatcttta tattgattga gcatagacgc aaccgtcgaa tatcgcttgt gatttacaat
    17581 ttgtgtatta agatgctcaa ctaatttggg tacatattcc gaatttagat ttgcaagtac
    17641 aacaattcca taattttgtt ttggatttag taaaataaat gatgaaaagt tatctagcgt
    17701 tcctgaatga aacactaaat gttcatcatt attggtaaac cagccggaag catatgcatt
    17761 ggcattaggt tcaccaattg ttgaagataa atttttatgt gattgttgaa ctaatgattt
    17821 gtatttatca ggtggattaa gttggaattt tatccaatgt tccaaatctt cagttgatgt
    17881 catcatatat gctgatggtg tatcccaaag gttaaattca ggtttagaga cgacaggtgt
    17941 cgaaccttgt aattcatagc caatagcatc atgttttgat ttgtaattgg tttgtttgaa
    18001 tgatgtatgt gtcatatgca aaggcttgag ccatgaattt gtaatatatt ttgtatagga
    18061 ttgcttcgta acgttttgga taattaaacc taataaatca tagttcatat ttgagtattc
    18121 aaattcttct ccgggcttat gatgtaattc atcacccata attgcacggg ttacatcatt
    18181 taaacgatta tttttgcttg tcacagaatc ttcgcttgta atatcactag gtataccact
    18241 tgtttgagcc aaaagttgct taatcgtaat agtttcattt tgaccattat agttcatttt
    18301 aaaatgaggc acatgtttgg atacggcatc atttaagttt aatcgacctt cttgagctaa
    18361 ttttaaaatt gcaagacctg tgaaagcttt cgtattagaa gcaatttcat actttgttgt
    18421 gggagaagct ttgacttttt tatcaacatc ttgataacca taacctttat ttaaaaaaac
    18481 tttgccattt tttactatta aaattgatgc tccaggaatg tgtccctttt gtaaatcatg
    18541 ctcgataatt gtatctattt gttgttgcga atcattggtt aaccgtgtct tcgtattgct
    18601 atttaatgtt atatatatag caattgaaat ggcgactaaa atgataatag aaatggatag
    18661 aaaatacagt tttttagtag tcataaacgt tcctttttat atgttaaatt tacttaagtg
    18721 cctaatgaga tttattttat tataccagtt ctattgaata aattaattaa agaggtatat
    18781 ttaccattat ttttgacacc ttatatgtta ataggtgtat aattacataa aaaatattaa
    18841 ttaactctct atactttttg gaaagatagg cgtatagtga tatccatcaa aaactataaa
    18901 aaggaatagg agattatgtt caatcaattt aaaagactta ttatagggca acctaaaaaa
    18961 aacagagaac taaaagacga aaagatatcg aagtttaaag ggttagcgat tctttcgtcg
    19021 gatgcattat cttcagtggc ttacggtccc gaacaaatac tgattacact atcagtagtg
    19081 ggtgcagttg caacttggta tactttaccg attgcaggtg ctgttctgat cttattagct
    19141 gctttaatta tgtcttatag gcaaattatt tatgcatatc ctaaaggtgg cggcgcgtat
    19201 atggtgtcca agaccaattt aggagaaaaa tggggattgc ttgcaggagg ctcattatta
    19261 gtcgattaca tactgactgt cgctgttagt atatcatctg gtgctgatgc atttgttgct
    19321 gcattcccaa gtttatatgg gcataaagta ctgattgcat gtttacttgt actttttatt
    19381 ttaatattaa atttacgtgg tttaactgaa tctgccaccg tattatccta tccagtatat
    19441 cttttcatta tagggttagt gatattaata tttataggta ctttccgtgt ggcgacagga
    19501 gatattcaac cacatatgca tgcatcagta ggaactgcgg ttcctggagt aacattattc
    19561 ttgctattaa aagcattttc atcaggggct tcatcgttaa cgggtgttga agctatttca
    19621 aatgcggtaa ccaattttag agagccaagt gctaaaaatg ctgtgaaaac gttaattgct
    19681 atgggttcaa ttttagcgtt tttattagtt ggtatcgttg gtttagcata tgtttatgga
    19741 attttgccac aaacggaaac gacagtttta tcacaattag ctatgcaaat atttggtgat
    19801 aatgctgcct tctattttgt acaggcaacg accgtgatga ttttggtatt agcagccaac
    19861 actggattta cagcttttcc gatgttagcg gcttcaatgt ctaaagataa atatatgcct
    19921 agaatgttta ctgttagagg tgatcgctta ggctactcta actcgattat tattttaggt
    19981 gtattagcca ttattttaat tattgtattt gatggtatga ctgaagattt aattccattg
    20041 tatgcggttg gtgtatttat tccatttaca ctagcacaat tcggtatggt tattaaatgg
    20101 attcatgaac gtcctaagaa ttggttaagt aagttgtctg tgaacttact tggtggtatc
    20161 gtgacattta ttgtttttat gattttactc ataactaaat ttagccaagt atggccgata
    20221 cttattttct tgccgttcgt ggtgattttc ttcttgaaaa ttaacaaaca ttatcgagat
    20281 atcgcagaac aattacgttc tgacattgat gttcttaatg tggacgtggt agatcgtaat
    20341 ttagcaattg tgccgataac aagtattaca acagcagttg ataagtctat ttattacgca
    20401 caaatgcttg cgaataatga tgtcatagca gtacatgtat catttggaga tgaagatgaa
    20461 aaagcattcc aagagaaatg gaaacgtcat ttcccagatg taagattagt tattttacat
    20521 tcggaatatc gcagtattat acgaccaatt tcacgtttta tcgataagat taatcgtaaa
    20581 gccaacgatc aaaactatat gattacagta gtgataccag aatttattac taagaaacgt
    20641 tggcataatt tattacacaa tcaaactagt ttgcgtatga aattgtattt aatttatcaa
    20701 aagaatgtta acgtatgtac aattccattt aaacttaaaa aatagattct ataatcaaat
    20761 cggattgatg aaaggttatt tacttttcat caatctgatt ttttgatgtt gaatataaaa
    20821 accccgtaca tatgagatga taatgacttt gtctcatatg tacgggggta ttgttatgtc
    20881 ttgcttaatg tttggactgt aaagacaaga caatttcgtt gtaattgata ttttctcgaa
    20941 tttgagctgc aacgatattg ttgattttat ttttgcgaat taattcatct aaaagatgac
    21001 gttgtaagta caatgcttcc aatttgtggt tatggaacat agcttgcggt atgatttgat
    21061 tgctgttttc ttgacgttct tttcgacgtt gtgcacgcca ctttaatgag cgactcatat
    21121 catagtaatg attaatgact aagctaacct caagtacatt tgtactattt tgttcttctt
    21181 ttaagcgtaa aataatattg tgattcacaa tacgcataat tttttgaaca tctttgaatg
    21241 aattttcaaa ttcttgcttc ttagattctt tatttaaggc tttgtagtgc ttcatcgctt
    21301 taagtctaag ccataacatc ttgaagaagt ttgcaaattt ttcaacaaat gatgcagtac
    21361 ggtgtgtctc tgttaattcg acaatattgc ggtaattatt aatatcctga tatgttgcct
    21421 tacctttatc aattaaacgc tcaagtgtag atgtttcgat tacttttgca atatcttcta
    21481 aacgtttgag ttcttttgta ttttgattat caggttctga atttaataaa aataacaatt
    21541 ctccatagta ttggtttaat actgggcgat aatttgtatc gtttttgtct tttttacttt
    21601 cgtttttaaa atgctggatc actttttgaa caatgaaaat tttggcagat tgataagtca
    21661 tacctttaaa agtagtatct tcggcagatg gtgtaattaa aggtaaaaca atttgcgcta
    21721 agattaaact aattaatacc atgaaagatg caataaacaa taaatcatta cggtattcga
    21781 atgcttgtcc ttttgtaatg ataaatggta atgtaagtgc cattgaaagt gaaattgtac
    21841 cgtgaatacc acacatggtc ataataaatg cgtaacgcac tcgagaaggt ggtgtttcat
    21901 gtgaatcatg ttcctcgtct agataagatt gtatattttt cgggaaataa aaatctttgt
    21961 accagaaata aacccaaaca aatcgacatg cataaattgc aatagcgatt aatatagtga
    22021 tcactattaa aaatgaaaag ttgtcaggct ctgtttggaa aatatctatg actacagtag
    22081 gaatcataaa acctaataca acaaatacaa aaccatttaa ggcataacta aatgtgttcc
    22141 aaatttgatg gtaattcatt tgtagttccg tttgtgctct aattaaacgg tcgcgttcta
    22201 aaccatggat tagacctgcg attacaactg caatgatacc tgaagcatga acttcttctg
    22261 ctaaaaagta tacgacaaaa ggagttaata attgaataaa agttaaggta ttgttatctt
    22321 ttaaaccttt attagcggtt aagtctatac gtattctaac gacaacgaat ccaataattg
    22381 caccaataag tacacctagt attgttgaaa tgataaattg ttcaacagct tggaataatg
    22441 aaaaggtacc agttactaat gcagtaacag caattttaaa tgaaatgata cctgctgcat
    22501 cattgagtaa agattcacct tctaaaatcg tcatagaacc tttaggtaat aattttccgc
    22561 gtgtaatagc agatactgct actgcatcag taggacataa aattgctgct attgcaaaag
    22621 cggctggcat tggtaaggca ggccaaatcc aatgtataaa atagccaaca ccgactacag
    22681 ttgcaaacac tagtgccatt gacattaata gtataggttt acgatattct aataattttg
    22741 ttcgagagac gtgggtacct tccacaaaaa gtagtggcgc gataacggca aacataaata
    22801 cttcagaatt gaattggaaa tcaacttgta ttggaataat gaaaataacg acacctaatg
    22861 caatttgaat aaaggcagta ggaatttgtg ggaatcgatt attgataacc gaactaataa
    22921 tcacagcaaa aataaaaatt aaaaatgctt ctaatagtgc catacaatac tcctcaaaat
    22981 tttaatagtt aatattttat cacttttaag gcataatgac atagatatat tgataaaatg
    23041 aagttatttt caaaaaaact ctagtatcgg ttgaactgat actagagcga gatgtttaaa
    23101 ttattgattg tcatatctga aatgaccgct gtcattttgt cgttgttcat acgcgagctt
    23161 ttcagcattc gttttgtatt ttttataaaa gaaaaataaa aatataaacc agaatggcga
    23221 aatataaata gctgctcttg tttcgtcact aaagaataat aaaatgaata caaagaagaa
    23281 gaacgctaga ataatgtaag caataggctt accaccaatc aacttaaatt tactgttttt
    23341 atgtgcctca ggatgctttt tcaaatacat tagataagcg acaataatca ttgcccaaac
    23401 aactaaaaac aacacagttg ataacgttgt aacgtatatg aatagttgaa ttgcattagg
    23461 gaaaatgtag tttaacaagg ctgctataag taatgcaatt gatgaaacta acattgataa
    23521 ataaggcacg ccatgactat tcgttttatt taaaacttta ggacctaacc cttgttgtga
    23581 cagtccgaat aagatacgac tattcgaata gataccacta tttgtagcag aggccgcggc
    23641 agttagcacg acaaagttaa cgacacctgc tgcaaatggt acgccgatta atgtaaagag
    23701 tttaacgaaa gggctactat ttggatcgat atcattccaa ggtatgactg acataattac
    23761 taatagacca ccgatataga ataataaaat acggataggt acattattaa ttgctttcgg
    23821 taaggttttt tcaggatctt tcgtttcacc agcagttaca cctataagtt caataccaat
    23881 gaatgaatat acagcaattt ggaatgacat taagaaacca aatgttccac cagggaacat
    23941 gccaccgtga ctgattaagt ttgtgaatga tgcatgtcca taatgtgttt taaatgagaa
    24001 aaagattaat acaagaccaa cgataaccat cgcaataatt gtgacaactt taataatcga
    24061 gaaccaaaac tccagttcac caaataatct ggcacctaat aagttgaagc taatcaagat
    24121 taaaacaata aatagcacgg taatccaatt tgggacttgt ggataccaaa atgcaaagta
    24181 ttgtcccatc gcagttaggt ctgacatact tgatacaatc caacataacc agtaagtcca
    24241 gccaatgaca aagccaccaa aagggcctaa atattcattt gcaatatcaa caaatgaatt
    24301 aaatcttgta ttgctcaaca acaattcgcc taatgcgcgc ataaaagcga atagtacaac
    24361 cccaataatc atgtatgtga ataacagtga tggaccagtt aaagatattg tttgacctga
    24421 tcctaggaat aacccagttc caattgcgcc cccaattgct attaattgga tgtgtctgtt
    24481 attcaactct ctatgcaatt ttcttgccat aattttcctc ctcaggataa agcaaataaa
    24541 aaagttgtgt gtaagcgttt tcttaattat aaata
//
LOCUS       DAXWFK010000021        24524 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.021, whole genome shotgun sequence.
ACCESSION   DAXWFK010000021 DAXWFK010000000
VERSION     DAXWFK010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 24524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24524
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.021"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(7..873)
                     /gene="fakB1"
                     /locus_tag="ACGEXS_000677"
     CDS             complement(7..873)
                     /gene="fakB1"
                     /locus_tag="ACGEXS_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB1"
                     /protein_id="HFF8011715.1"
                     /translation="MKIAVMTDSTSYLSQDLIDKYNIQIAPLSVTFDDGKNFTESNEI
                     AIEEFYNKMASSQTIPTTSQPAIGEWITKYEMLRDQGYTDIIVICLSSGISGSYQSSY
                     QAGEMVEGVNVHAFDSKLAAMIEGCYVLRAIEMVEEGYEPQQIIDDLTNMREHTGAYL
                     IVDDLKNLQKSGRITGAQAWVGTLLKMKPVLKFEDGKIIPEEKVRTKKRAIQTLEKKV
                     LDIVKDFEEVTLFVINGDHFEDGQALYKKLQDDCPSAYQVAYSEFGPVVAAHLGSGGL
                     GLGYVGRKIRLT"
     gene            1017..1658
                     /locus_tag="ACGEXS_000678"
     CDS             1017..1658
                     /locus_tag="ACGEXS_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFF8011716.1"
                     /translation="MTQNIITVKKEHIIENVISKSRFIAHIKPVQNEDEAKAFIAAIK
                     KEHKDATHNCSAYTIGPEMDIQKANDDGEPTGTAGVPMLDILKKLDVHNACVVVTRYF
                     GGIKLGGGGLIRAYSGAVRDVIYDIGRVELREAVPCIVTLNYDQTGKFEYELASTHFM
                     LRNQTYTDKVSYYIDVVKSDYEAFINFLNQMTAGNFDLTEETPKQLPFDIPTE"
     gene            complement(1828..2883)
                     /locus_tag="ACGEXS_000679"
     CDS             complement(1828..2883)
                     /locus_tag="ACGEXS_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFF8011717.1"
                     /translation="MVTLLLVAVTMIVSLTITPIVIAISKRLNLVDKPNFRKVHTKPI
                     SVMGGTVILFSFLIGIWIGHPIETEIKPLIIGAIIMYVLGLVDDIYDLKPYIKLAGQI
                     AAALVVAFYGVTIDFISLPMGTTIHFGFLSIPITVIWIVAITNAINLIDGLDGLASGV
                     SAIGLITIGFIAILQANIFITMICCVLLGSLIGFLFYNFHPAKIFLGDSGALMIGFII
                     GFLSLLGFKNITIIALFFPIVILAVPFIDTLFAMIRRVKKGQHIMQADKSHLHHKLLA
                     LGYTHRQTVLLIYSISILFSLSSIILYVSPPLGVVLMFVLIIFSIELIVEFTGLIDNN
                     YRPILNLISRKSSHKEE"
     gene            3201..4271
                     /locus_tag="ACGEXS_000680"
     CDS             3201..4271
                     /locus_tag="ACGEXS_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFF8011718.1"
                     /translation="MFEAFIYNISVIVAGIYLFHRLQYSENKRMVFSKAYVTVLMTIV
                     SLLLSVYPIPYREDYLIHLTFVPLLFLGRFTNMVYTLSATVIVAIVEIVVFNNSIMYG
                     VTLIVIAAVTSAIGPFLKQNDVLSLLILNVVTIIILFGVALVSPIYTLSEVIILIPIS
                     LIITLASAITFVDIWHFFSLVNRYENEDKYDYLTGLGNVKEFDRHLNEISRKAEKEHQ
                     SIALLLIDIDGFKDVNDTYSHKSGDAVLKQMSQLLKNYVPNQFKIFRNGGEEFSVVIH
                     NYSLDQSVKLAENIRSGVEKSSFHLPNKEVIKLSVSIGVGYLTDDDPKSQRKVFKDAD
                     DMVHVAKNQGRNKVMFNPIINL"
     gene            4467..5228
                     /locus_tag="ACGEXS_000681"
     CDS             4467..5228
                     /locus_tag="ACGEXS_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFF8011719.1"
                     /translation="MPDSITIIDENKVIDVVLIAGRILLESGAETYRVEDTMNRIAHS
                     YGLHNTYSFVSSTAIIFSLNDRTSTRLIRVQERTTDLEKIALTNSLSRKISNKELTID
                     EAKSEFIHLQHASLQYSFLTNFFAAAIACGFFLFMFGGVASDCWIAVIAGGSAFLTFS
                     FVQRYIQIKFFSEFVAAAVVISIAATFTKLGIATNQDIITIASVMPLVPGILITNAIR
                     DLLAGELLAGMSRGVEAALTAFAIGAGVAIVLLII"
     gene            5246..5740
                     /locus_tag="ACGEXS_000682"
     CDS             5246..5740
                     /locus_tag="ACGEXS_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFF8011720.1"
                     /translation="MLFYLFHFTISFISTVLFSIIFNAPKRLLVACGFVGAIAWTIYQ
                     LTVDLEFGKVGASFLGSLILGLMSHTMSRRYKRPVIIFIVPGIIPLVPGGAAYQATRF
                     LVSNDYTSAVNTFLEVTLISGAIAFGILVSEILYYLYTRIKQLYGKIKGKTYKKSYNM
                     NNRV"
     gene            5754..6980
                     /gene="pepT"
                     /locus_tag="ACGEXS_000683"
     CDS             5754..6980
                     /gene="pepT"
                     /locus_tag="ACGEXS_000683"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFF8011721.1"
                     /translation="MKNQLIDRLTRYTTIDTQSDPKSTTTPSTEKQWDLLHLLEKELQ
                     QLGLPTDLDENGYLFATLESNIDVDVPTVGFLAHVDTSPDFNASNVKPQIIENYDGKP
                     YKLGNTKRVLDPKVFPELNSLVGHTLMVTDGTSLLGADDKAGIVEIMEAICYLQEHPE
                     IKHGTIRIGFTPDEEIGRGPHKFDVDRFNADFAYTMDGSQYGELQYESFNAAEAVITC
                     HGINVHPGSAKNAMVNAIRLGEQFDSLLPDSEVPERTEGYEGFYHLMNFEGTVEKATL
                     QYIIRDHDKKQFELRKKRILEIRDDINAHFENYPVKVDISDQYFNMAEKILPLPHIID
                     IPKRVFAKLDIPANTEPIRGGTDGSQLSFMGLPTPNIFTGCGNFHGPYEYASIDVMEK
                     AVQVIIGIVEDIAENH"
     gene            complement(7169..8293)
                     /locus_tag="ACGEXS_000684"
     CDS             complement(7169..8293)
                     /locus_tag="ACGEXS_000684"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFF8011722.1"
                     /translation="MKVLVAMDEFHGIISSYQANRYVEEAVASQIETADVVQVPLFNG
                     RHELLDSVFLWQSGQKYRIPVHDADMNEVEGVYGQTDTGMTVIEGNLFLKGKKPIVER
                     TSYGLGEMIKHALDNDAKHVVISLGGIDSFDAGAGMLQALGAQFYDDEGRVVDMRQGA
                     GVIKYIRRMDMSNLHPKMETARIQVMSDFSSRLYGKQSEIMQTYDAHQLNHNQAAEID
                     NLIWYFSELFKSELKIAIGPVERGGAGGGIAAVLNGLYQAEILTSHALVDQLTHLENL
                     VEQADLIIFGEGLNENDQLLETTTLRIAELCHKHQKVAIAICATAEKFDLFESQGVTA
                     MFNTFIDMPETYTDFKMGLQIRHYTVQSLKLLKTHFNVEV"
     gene            complement(8718..9038)
                     /gene="ytxJ"
                     /locus_tag="ACGEXS_000685"
     CDS             complement(8718..9038)
                     /gene="ytxJ"
                     /locus_tag="ACGEXS_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HFF8011723.1"
                     /translation="MAIKLSSIDQFEQVIEENKYVFVLKHSETCPISANAYDQFNKFL
                     YERDMDGYYLIVQQERDLSDYIAKKTNVKHESPQAFYFVNGEMVWNRDHGDINVSSLA
                     QAEE"
     gene            complement(9192..10070)
                     /locus_tag="ACGEXS_000686"
     CDS             complement(9192..10070)
                     /locus_tag="ACGEXS_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EMYY motif lipoprotein"
                     /protein_id="HFF8011724.1"
                     /translation="MKKIVIIAVLAILFVVISACGNKEKEAQHQFTKQFKDVEQKQKE
                     LQHVMDNIHLKEIDHLSKTDTTDKNSKEFKALQEDVKNHLIPKFEAYYKSAKNLPDDT
                     MKVKKLKKEYMTLANEKKDAIYQLKKFIGLCNQSIKYNEDILDYTKQFEKNRYKVESE
                     IKLADNKSEATNLTTKLEHNNKALRDTAKKNLDDSKENEVKGAIKNHIMPMIEKQITD
                     INQTNISDKHVNNARKNAIEMYYSLQNYYNTRIETIKVSEKLSKVDVDKLPKKGIDIT
                     HGDKAFEKKLEKLEEK"
     gene            10190..10708
                     /locus_tag="ACGEXS_000687"
     CDS             10190..10708
                     /locus_tag="ACGEXS_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HFF8011725.1"
                     /translation="MYSNVQPFINSDLTNCFQSQYTEIESLLFNLLDSPVKYTKHIGG
                     THHFKYSTEPILDILVGVENLHDITSLDEKRLNYVGFYRLHHKYTKKVMMAKFNNFHE
                     LKQQVRLHIIQMNTQTFESYLALDYLLSTNDDIALQFNTKKHQILQHSLTIRQYEMQK
                     QQYFEKLCKKYL"
     gene            10835..11758
                     /gene="murB"
                     /locus_tag="ACGEXS_000688"
     CDS             10835..11758
                     /gene="murB"
                     /locus_tag="ACGEXS_000688"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFF8011726.1"
                     /translation="MINKDIYQALQQLIPNEKIKVDEPLKRYTYTKTGGNADFYITPT
                     KNEEVQAVVKYAYQNEIPVTYLGNGSNIIIREGGIRGIVISLLSLDHIEVSDDAIIAG
                     SGAAIIDVSRVARDYALTGLEFACGIPGSIGGAVYMNAGAYGGEVKDCIDYALCVNEQ
                     GSLIKLTTKELELDYRNSIIQKEHLVVLEAAFTLAPGKMTEIQAKMDDLTERRESKQP
                     LEYPSCGSVFQRPPGHFAGKLIQDSNLQGHRIGGVEVSTKHAGFMVNVDNGTATDYEN
                     LIHYVQKTVKEKFGIELNREVRIIGEHPKES"
     gene            11776..12090
                     /locus_tag="ACGEXS_000689"
     CDS             11776..12090
                     /locus_tag="ACGEXS_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHY zinc finger protein"
                     /protein_id="HFF8011727.1"
                     /translation="MPKVYGSLIDTETRCRHYFTEEDIIAIKFKCCNKYYPCYKCHNE
                     FEKHAIKRWSEPSFNEKAILCGVCKHELTINEYMMVERCPNCQSRFNNRCKYHYHIYF
                     EI"
     gene            complement(12407..13435)
                     /locus_tag="ACGEXS_000690"
     CDS             complement(12407..13435)
                     /locus_tag="ACGEXS_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFF8011728.1"
                     /translation="MKKTVLYLVLAVMFLLAACGNNSDKEQSKSETKGSKDTVKIENN
                     YKMRGEKKDGSDAKKVKETVEVPKNPKNAVVLDYGALDVMKEMGLSDKVKALPKGEGG
                     KSLPNFLESFKDDKYTNVGNLKEVNFDKIAATKPEVIFISGRTANQKNLDEFKKAAPK
                     AKIVYVGADEKNLIGSMKQNTENIGKIYDKEDKAKELNKDLDNKIASMKDKTKNFNKT
                     VMYLLVNEGELSTFGPKGRFGGLVYDTLGFNAVDKKVSNSNHGQNVSNEYVNKENPDV
                     ILAMDRGQAISGKSTAKQALNNPVLKNVKAIKEDKVYNLDPKLWYFAAGSTTTTIKQI
                     EELDKVVK"
     gene            complement(13554..14315)
                     /locus_tag="ACGEXS_000691"
     CDS             complement(13554..14315)
                     /locus_tag="ACGEXS_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8011729.1"
                     /translation="MIQVENLTKTINNQMILEDISIDIEKGKLTSLIGPNGAGKSTLL
                     SAICRLIRFENGEVKIDGQLMSDYKNNDLSKKISILKQTNHTEMNITVEQLVNFGRFP
                     YSKGRLTKEDHDIVNDALDLLQLQDIRNRNIKSLSGGQRQRAYIAMTIAQDTEYILLD
                     EPLNNLDMKHAVQIMQTLKMLAHKMNKAIVIVLHDINFASCYSDQIVALKNGQLVKSD
                     LKDNVIQSSVLSDLYDMNIQIEHIRNQRICLYFKD"
     gene            complement(14312..15268)
                     /locus_tag="ACGEXS_000692"
     CDS             complement(14312..15268)
                     /locus_tag="ACGEXS_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFF8011730.1"
                     /translation="MRNNNKKIMLLIAVTLLISMLYLFVGIDFEIFEYQFSSRLRKFI
                     LIILVGAAIATSVVIFQAITNNRLLTPSIMGLDAVYLFIKVLPVFLFGIQSVWVTNVY
                     LNFILTLITMVLFALILFQGIFKIGHFSIYFILLIGVLLGTFFRSITGFIQLIMDPES
                     FLAIQSSMFANFNASNSNLVTFSAVLLVILLVITILLLPYLDVLLLGRVEAINLGISY
                     EKLTRILLVIVSVLVSVSTALVGPITFLGLLTVNLAHELMKTYEHKYILIATICLSWI
                     SLFSAQWVVENVFEATTEMSILIDLIGGSYFIYLLVRRRNAQ"
     gene            complement(15255..16226)
                     /locus_tag="ACGEXS_000693"
     CDS             complement(15255..16226)
                     /locus_tag="ACGEXS_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8011731.1"
                     /translation="MKYLLKGNILLLLLILLTIISLFIGVSELSIKGLLHLTESQRNI
                     LFSSRIPRTMSILIAGSSLALAGLIMQQMMQNKFVSPTTAGTMEWAKLGILIALLFFP
                     TGHILLKLVFAVICSICGTFLFVKIIDFIKVKDVIFVPLLGIMMGGIVASFTTFISLR
                     TNAVQSIGNWLNGNFAIITSGRYEILYLSIPLLALTYLFANHFTIVGMGKDFTNNLGL
                     SYEKLINIALFITATITALVVVTVGTLPFLGLVIPNIISIYRGDHLKNAIPHTMMLGA
                     IFVLFSDIVGRIVVYPYEINIGLTIGVFGTIIFLILLMKGRKNYAQQ"
     gene            complement(16265..16420)
                     /locus_tag="ACGEXS_000694"
     CDS             complement(16265..16420)
                     /locus_tag="ACGEXS_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011732.1"
                     /translation="MFYFTLSKQNTHSEKLNFSLIANINKIDTKLFERLLKLWSKNAT
                     INEKYYQ"
     gene            complement(16822..17793)
                     /gene="nrdF"
                     /locus_tag="ACGEXS_000695"
     CDS             complement(16822..17793)
                     /gene="nrdF"
                     /locus_tag="ACGEXS_000695"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFF8011733.1"
                     /translation="MIAVNWNTQEDMTNMFWRQNISQMWVETEFKVSKDIASWKTLSE
                     AEQDTFKKALAGLTGLDTHQADDGMPLVMLHTTDLRKKAVYSFMAMMEQIHAKSYSHI
                     FTTLLPSSETNYLLDEWVLEEPHLKYKSDKIVANYHKLWGKEASIYDQYMARVTSVFL
                     ETFLFFSGFYYPLYLAGQGKMTTSGEIIRKILLDESIHGVFTGLDAQHLRNELSESEK
                     QKADQEMYKLLNDLYLNEESYTKMLYDDLGITEDVLNYVKYNGNKALSNLGFEPYFEE
                     REFNPIIENALDTTTKNHDFFSVKGDGYVLALNVEALQDDDFVFDNK"
     gene            complement(17911..20016)
                     /gene="nrdE"
                     /locus_tag="ACGEXS_000696"
     CDS             complement(17911..20016)
                     /gene="nrdE"
                     /locus_tag="ACGEXS_000696"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000855513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFF8011734.1"
                     /translation="MKTMEEKKYNHIELNNEVTKRREDGFFSLEKDQEALVAYLEEVK
                     DKTIFFDTEIERLRYLVDNDFYFNVFDIYSEADLIEITDYAKSIPFNFASYMSASKFF
                     KDYALKTNDKSQYLEDYNQHVAIVALYLANGNKAQAKQFISAMVEQRYQPATPTFLNA
                     GRARRGELVSCFLLEVDDSLNSINFIDSTAKQLSKIGGGVAINLSKLRARGEAIKGIK
                     GVAKGVLPIAKSLEGGFSYADQLGQRPGAGAVYLNIFHYDVEEFLDTKKVNADEDLRL
                     STISTGLIVPSKFFDLAKEGKDFYMFAPHTVKEEYGVTLDDIDLEKYYDDMVANPNVE
                     KKKKNAREMLNLIAQTQLQSGYPYLMFKDNANRVHPNSNIGQIKMSNLCTEIFQLQET
                     SIINDYGIEDEIKRDISCNLGSLNIVNVMESGKFRDSVHSGMDALTVVSDVANIQNAP
                     GVRKANSELHSVGLGVMNLHGYLAKNKIGYESEEAKDFANIFFMMMNFYSIERSMEIA
                     KERGIKYQDFEKSDYANGKYFEFYTTQEFEPQFEKVRELFDGMAIPTSEDWKKLQQDV
                     EQYGLYHAYRLAIAPTQSISYVQNATSSVMPIVDQIERRTYGNAETFYPMPFLSPQTM
                     WYYKSAFNTDQMKLIDLIATIQTHIDQGISTILYVNSEISTRELARLYVYAHYKGLKS
                     LYYTRNKLLSVEECTSCSI"
     gene            complement(19979..20377)
                     /gene="nrdI"
                     /locus_tag="ACGEXS_000697"
     CDS             complement(19979..20377)
                     /gene="nrdI"
                     /locus_tag="ACGEXS_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFF8011735.1"
                     /translation="MKIIYFSFTGNVRRFIKRTELENTLEITAENCMEPVHEPFIIVT
                     GTIGFGEVPEPVQSFLEVNHQYIRGVAASGNRNWGLNFAKAGRTISEEYNVPLLMKFE
                     LHGKNKDVIEFKNKVGNFNENHGREKVQSY"
     gene            21178..22044
                     /locus_tag="ACGEXS_000698"
     CDS             21178..22044
                     /locus_tag="ACGEXS_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001594836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFF8011736.1"
                     /translation="MNPKVKGIIAILISAIGFSFMSVFFRLAGDLPVFQKSLARNLVA
                     MFIPLFFIYKYHQPMFGRLSSQPLLITRSTLGLIGVLLNIYAIDHMVLSDADSLMKLN
                     PFWTILLSIVFLHEKVRKYQITAMIIAILGMLLIVKPEFSSSMIPSLAGLFSGIFAAS
                     AYTCVRALSTREAPYTIVFYFSLFSVIVLIPFTAYTYEPMSQMQILYLLGAGLAAAVG
                     QIGVTLAYSFAAAKDISIFTYASIIFTAILGFILFGESPDFYATLGYVVIIGASYYMF
                     EKARRDAKIIKK"
     gene            22064..22564
                     /gene="queF"
                     /locus_tag="ACGEXS_000699"
     CDS             22064..22564
                     /gene="queF"
                     /locus_tag="ACGEXS_000699"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046857 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors, with NAD or NADP
                     as acceptor [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HFF8011737.1"
                     /translation="MAHGRQQDELQDITLLGNQDNTYNFDYRPDVLESFDNKHQGRDY
                     FVKFNCPEFTSLCPITGQPDFATIYISYIPNVKMVESKSLKLYLFSFRNHGDFHEDCM
                     NIIMNDLIELMDPHYIEVWGKFTPRGGISIDPYTNYGRPNSKYEKMAEHRLMNHDLYP
                     EKIDNR"
     gene            22594..22839
                     /locus_tag="ACGEXS_000700"
     CDS             22594..22839
                     /locus_tag="ACGEXS_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001788366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011738.1"
                     /translation="MIFLTYWCVFHLAYIIGYIKITFYTKVHQKNISTRIKQHFYRNL
                     LHFNVNKYIFILSLINCARLITEYSQYFYFFVICWNI"
     gene            22905..24410
                     /locus_tag="ACGEXS_000701"
     CDS             22905..24410
                     /locus_tag="ACGEXS_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFF8011739.1"
                     /translation="MTQQNSHGNQIQDIPQTGFFGHPRGLGVLFFVEFWERFSYYGMR
                     ALLIFYMYFAVTDNGLGIDKTTAMSIMSVYGSLIYMTSIPGGWIADRITGTRGATLLG
                     AVFIIIGHICLSLPFALIGLFTSMFFIIIGSGLMKPNISNIVGRLYPENDRRMDAGFV
                     IFYMSVNMGALLSPIILQHFVNVKNFHGGFLIAAVGMALGLVWYVLFNRKNLGSVGMK
                     PTNPLTPAEKKKYGLIIGSVVLAIVLIIVIGALTNSLSFNLVSNTVLVLGIALPIIYF
                     TLIIRSKDVTDTERSRVKAFIPLFILGMVFWAIQEQGSNVLNIYGIEHSDMKLNLFGW
                     KTNFGEAIFQSINPLFILLLAPIISLLWQKLGTKQPSLPVKFAIGTFLAGASYILIGI
                     VGYASGSSNFSVNWVILSYIICVIGELCLSPTGNSAAVKLAPKAFNAQMMSIWYLTNA
                     SAQAINGTLVKLIEPLGQTNYFIFLGVVAIIVTTIVLAFSPLIIKAMKGIR"
BASE COUNT         8120 a         4191 c         3578 g         8635 t
ORIGIN      
        1 ttataattat gttaatctta tttttctgcc aacatagcct aaacctaatc caccagaacc
       61 taaatgtgct gcaacaactg gaccaaactc agagtatgct acttgataag ctgaaggaca
      121 atcatcttgt aactttttgt ataacgcttg accatcttcg aaatgatctc catttatgac
      181 aaataaagtt acttcttcaa agtcttttac aatatctaat actttctttt ctaatgtttg
      241 aatggcacgc tttttagtac gaactttttc ttctggtata atcttgccat cttcaaactt
      301 aagaactggc ttcattttca ataatgttcc aacccatgct tgagcaccag taattcgacc
      361 acttttttgt aaattcttta agtcatcaac aattaaatat gcacctgtgt gttcacgcat
      421 attagttaaa tcatcaataa tttgctgtgg ctcgtatcct tcttcaacca tttcaatagc
      481 acgtaataca taacatcctt caatcattgc tgcaagctta ctatcaaatg catgtacatt
      541 aacaccttca accatttccc ctgcttgata actagattga taacttccac taatcccact
      601 tgataagcaa atgacaatga tatctgtgta accttgatct cttaacattt catatttagt
      661 aatccattcg ccaattgctg gttggcttgt tgttggaatc gtttgagacg atgccatttt
      721 attataaaat tcttcaattg ctatttcatt actttctgta aagttcttcc catcatcaaa
      781 agtcacactt aatggcgcta tttgaatatt atatttatcg attaagtcct gcgacagata
      841 acttgtagaa tcggtcatca cagcaatttt catagtcgtc ctcctaaaat atactcatag
      901 caatgataca atatttcagt taaaaagaaa atggcaatca aacgatttat agtcatgtca
      961 ttttcagtgt ataatgaaga ttaaataatc ttaagcttat gaattaagga gttattatga
     1021 cacaaaatat cattactgtt aaaaaagaac acattattga aaatgtgatt agtaaatcaa
     1081 gattcattgc tcatatcaaa cctgttcaaa atgaagatga agctaaagca tttattgctg
     1141 caataaaaaa agaacataaa gatgccactc ataattgttc tgcatatact attggacctg
     1201 aaatggatat tcaaaaagcg aatgatgatg gtgaaccgac aggaactgcc ggtgtcccaa
     1261 tgttagacat attaaaaaag ctcgatgttc ataatgcgtg tgtcgtagta acaagatatt
     1321 ttggtggtat taaattaggt ggtggcggtt taattcgcgc ctatagcggt gctgttagag
     1381 acgtcattta tgatatcggg cgtgtcgaac tacgagaagc agtaccatgt atagtaacac
     1441 tgaattatga tcaaactggt aaatttgaat atgaattagc ttcaactcat tttatgttaa
     1501 gaaaccaaac ttacactgat aaggtaagct actatatcga tgtcgttaaa agtgactatg
     1561 aagcgtttat taacttttta aatcaaatga ctgcaggaaa tttcgatttg actgaagaaa
     1621 ctccaaagca gttacctttc gatataccaa ctgaataaaa ataaaaagca ctatcataag
     1681 acggtaaata tcaattgcgt attattaaac gacaattgaa gtacacgttt atggcagtgc
     1741 tttttatatt tgaaatttaa tacaaaaaaa ttgtaaagag taaagccata taatacaaat
     1801 tatcccatac agctatgctt tcattcccta ttcctcttta tgagatgact tacgactaat
     1861 taaatttaat attggtcggt agttgttatc tattaatcct gtaaattcaa caattaattc
     1921 aatactaaat atgattaata caaacattaa tacaacacct aatggtggcg atacatacaa
     1981 aataatgctc gaaagactaa ataaaataga gattgaatag attaataata ctgtttgtct
     2041 atgtgtgtag cctaaagcta atagtttatg atgcaaatgc gatttatcag cttgcattat
     2101 atgctgccct tttttcacac gtcgaatcat tgcgaacaaa gtatcaatga atggaactgc
     2161 taagataaca attgggaaga acaatgcaat aattgtaata tttttgaatc cgagtaaaga
     2221 aaggaatccg atgataaatc caatcattaa agccccacta tcacctaaaa atattttggc
     2281 aggatggaaa ttgtaaaata aaaacccaat taaagagcct aataaaacac aacaaatcat
     2341 cgttatgaaa atattagctt gtaaaattgc aatgaaccct attgtaatga gtccgattgc
     2401 agaaacaccc gacgccaaac catcgagtcc atcaattaag ttaattgcat ttgtaatagc
     2461 aacaatccaa atcacagtaa ttggaatact aagaaatcca aaatgaatcg ttgtacccat
     2521 tggcaacgaa ataaaatcaa tagtcacacc ataaaaagca actactaagg cagcggcaat
     2581 ttgaccagcc aattttatat acggtttcaa atcgtagata tcatctacaa gcccaagtac
     2641 gtacataata atcgcaccaa taataagtgg tttgatttct gtttcaatag gatgaccaat
     2701 ccaaatacct attaaaaatg aaaagagaat cactgtacca cccataactg aaataggttt
     2761 agtgtgtact tttctaaaat ttggtttatc aactaaattt aatcttttcg atattgcaat
     2821 aacaattggt gttatcgtca aactgacaat cattgttact gcaactagta ataatgtaac
     2881 catttattca ccttcatcga tattaattgt ttttaacgtt atatttattt aatattgtct
     2941 ttatagaatg atatatctat tttctacgta tatcttatag ctttattata aattaattcg
     3001 ttcaatcaat gatatcatat ttacaactat acgattcatt tcttatatat cgcctattat
     3061 tcattttttc atcatatcat ttaaggcaac attttaacat caaagtctat atcatacatt
     3121 aattttatat gtaaactaat gacaaattgt aagttttcac ttacaatagc tagtggtcaa
     3181 ttaaaggagt cgattcagac atgttcgaag catttatata caatatatct gttatagtcg
     3241 ctggaatcta tttattccat cgcttacagt attcagaaaa caaacgtatg gttttttcta
     3301 aggcttatgt cactgtttta atgacaatcg tatcattatt attatctgtc taccctatcc
     3361 cttatcgtga agattattta attcacttaa cattcgttcc tttattgttt ttaggtagat
     3421 ttacaaatat ggtctataca ttatctgcca cagtaattgt agcaattgtt gaaattgtag
     3481 tatttaacaa ctcgattatg tacggtgtta cgttgattgt tattgctgca gtaacaagtg
     3541 ctattggacc atttttaaaa caaaatgatg tgctatcatt attaatactt aatgttgtta
     3601 caataatcat tttatttggt gtcgcgttag tgagtcctat ttacacacta tcggaagtta
     3661 tcattttaat accaatttca ttaattatca ctttagcttc agcgataaca tttgtcgata
     3721 tttggcattt cttctcactc gtaaatcgtt atgaaaacga agataaatat gattacttaa
     3781 caggcttagg taatgtaaaa gaatttgata gacatttaaa tgaaatttca cgaaaagctg
     3841 aaaaagaaca tcaaagtatc gcattattat taatcgatat cgatggattt aaagatgtca
     3901 atgataccta ctcacacaaa tcaggtgatg ctgtattaaa acaaatgtct caattactta
     3961 aaaactatgt gccgaatcaa tttaaaattt ttagaaatgg tggcgaagag ttctcagttg
     4021 tcattcacaa ttattcactt gatcaaagtg ttaaattagc tgaaaatatt cgttcgggtg
     4081 ttgaaaaatc atcattccat ttaccaaaca aagaagttat taaattgtca gtgtctatcg
     4141 gtgttggtta tttaacagat gacgatccaa aatcacagcg taaagtgttt aaagatgcag
     4201 acgatatggt gcatgtagcc aaaaatcaag ggcgaaacaa agtaatgttt aaccctatta
     4261 tcaatttata acaacgacaa aactgttaat cctttgctat tattaaatca tagtataata
     4321 gcaaagatta acagtttttc ttatttaaat ttgaaccata caagttcatc atactaacac
     4381 aaagtctcac tattgaaagt tgtaacaaca agttgtaaac ttcaattgtt caaataaata
     4441 acacatatca ttggaggtga attgatatgc cagattcaat cacaattata gatgaaaaca
     4501 aagtgattga tgttgtatta attgcaggta gaattttact tgaatcaggt gctgaaacat
     4561 atcgagttga agatacaatg aaccgtatcg cacatagtta tggtcttcat aatacatata
     4621 gttttgtcag ttcaactgca attatttttt cattaaacga tcgaacaagt acaagattaa
     4681 ttcgtgtaca agagcgtaca acagatttag agaaaatcgc tttaacgaat agtctttcac
     4741 gtaaaatatc aaataaagaa ctcacaattg atgaagctaa atctgaattt attcatttac
     4801 agcatgcatc attacaatat tcttttttaa cgaatttctt tgcagctgcc attgcatgtg
     4861 gcttcttcct atttatgttt ggtggtgtcg catcagattg ttggattgca gtcattgctg
     4921 gcggatccgc atttttaaca ttcagctttg tgcaacgcta tatacaaatt aaatttttct
     4981 cagagtttgt tgcagccgct gtggtcatat ccattgcagc cacatttact aaattaggca
     5041 ttgcaaccaa tcaagatatc attactatag ccagtgtcat gccacttgtc cctggtatat
     5101 taattaccaa tgccattcga gatttattag cgggtgaatt acttgcaggt atgtctcgcg
     5161 gtgttgaagc tgcattaaca gcattcgcaa tcggtgctgg tgtcgcaatc gttttattaa
     5221 tcatttaagg aaggactgtt aaagcatgct attttattta tttcatttta caatcagctt
     5281 tatatcaaca gtacttttct ctatcatttt caatgcaccc aaacgcctct tagtagcatg
     5341 tggatttgtg ggtgccattg catggacgat ttaccaatta acggtagatt tagagtttgg
     5401 aaaagttggc gcttcatttt tgggaagctt aattttaggc ttaatgagtc atactatgag
     5461 tcgcagatat aaacgaccgg taattatatt catagtgcca ggcattatac cattagtacc
     5521 tggtggtgca gcttatcaag cgactcgttt tttagtatca aatgattata caagtgctgt
     5581 aaatacattt ttagaagtta cactgatttc aggtgcgatt gctttcggta tattagtttc
     5641 tgaaattcta tattacctat acacacgtat caaacaactg tatggtaaaa tcaaaggtaa
     5701 gacatataaa aaatcttaca acatgaataa tagagtttaa ggggtcgaat ataatgaaga
     5761 accaattgat agatagatta acaagatata cgacaattga tacgcaatct gatccaaaat
     5821 ctacaacaac accttcaact gaaaagcaat gggatttgtt acatttatta gaaaaagaat
     5881 tacaacaatt aggattgcca actgatttag atgaaaatgg ctacttattt gctacattag
     5941 aaagtaacat cgatgtcgac gtcccaacag ttggattttt ggcgcatgtt gatacatcac
     6001 ctgatttcaa tgcttcaaat gttaaaccgc aaattattga aaactatgat ggtaagccat
     6061 acaaactagg caatactaaa cgtgtcttag atcctaaagt atttccagaa cttaacagtt
     6121 tagttggtca tacattaatg gtaactgatg gtacatcgtt actaggtgca gacgataaag
     6181 cgggtattgt ggaaattatg gaagctattt gttatttaca agaacatcca gaaattaaac
     6241 atggtaccat tcgcattgga tttacaccag acgaagaaat cggtcgtggt ccacataaat
     6301 ttgatgttga ccgcttcaat gctgattttg cttatactat ggatggtagt caatatggag
     6361 aattacaata tgaaagcttt aacgctgctg aagcggttat tacatgccac ggtataaatg
     6421 ttcatcctgg ttcagctaaa aatgcaatgg taaacgcaat acgtttaggt gaacaattcg
     6481 atagtttgct acctgatagt gaagttccgg agcgaacaga aggatacgaa ggcttttatc
     6541 acttaatgaa ctttgaagga actgttgaaa aagcaacttt gcaatacatt attcgtgatc
     6601 atgataaaaa acaattcgaa ttgcgtaaga aacgtatttt agaaatacgt gacgatatca
     6661 atgcccattt tgaaaattat ccagttaaag ttgatatatc ggatcaatat ttcaatatgg
     6721 cagaaaaaat attaccattg cctcatatta ttgatatacc taaacgtgtc tttgccaaat
     6781 tagatattcc agcaaacact gaacctattc gcggtggtac agatggttca caattgtcat
     6841 ttatggggtt accaactcca aacatcttta caggttgcgg taacttccac ggcccatatg
     6901 aatatgcatc cattgatgtt atggaaaaag cagtacaagt aatcattgga attgtagaag
     6961 atatcgctga aaatcactaa aaaccaacga ttatatacta gttcaactca ggcttaatta
     7021 atatataaaa atcacgctaa cattcagtgc aatgatagcg tgatttttca tattttttac
     7081 gtatactttc gatcttctaa ttcgttttgc taaccatcat aaatgttatt aaccatcatg
     7141 acagcatcac caatttagtc cttctttact aaacctcaac attaaaatgt gttttcaaca
     7201 gttttaaaga ctgaaccgta taatgcctaa tttgtaaccc cattttaaag tcagtataag
     7261 tttctggcat atcgataaat gtattaaaca ttgctgtaac cccttgtgat tcaaataaat
     7321 caaacttttc agcagttgca caaattgcaa tggcaacctt ttgatgttta tgacaaagtt
     7381 ctgcaatacg caatgttgtc gtttctagca actgatcatt ttcatttaat ccttctccaa
     7441 aaataattaa atccgcttgt tcaactaaat tttctaaatg tgttagttgg tctactaatg
     7501 catgactggt taatatttca gcttgataca gtccattcaa gactgctgca attccaccac
     7561 cagcaccacc acgttcaact ggaccaattg caattttcaa ttcactttta aataactcac
     7621 taaaatacca aattaaatta tcgatttctg ctgcttgatt atgattcaac tgatgcgcat
     7681 cataagtttg catgatttca ctttgcttac catataatcg acttgaaaaa tccgacatta
     7741 cttgaattct tgctgtttcc attttagggt gtaagttcga catatccata cgacgaatat
     7801 atttaattac accagcacct tgtctcatat ctacgacacg cccttcgtca tcatagaatt
     7861 gagcacctaa tgcttgtaac atacctgcac cagcatcaaa actatcaatc ccacctagtg
     7921 aaattacaac atgttttgcg tcgttatcta atgcatgttt aatcatttct cctaaaccat
     7981 aacttgttcg ttcaacaatt ggttttttac cttttaaaaa taaattcccc tcgataacgg
     8041 tcatccctgt atcagtttgt ccgtaaacac cttcaacttc attcatatct gcatcatgta
     8101 ctggtatacg atacttttgc ccagattgcc ataaaaatac agaatctaat aattcatgtc
     8161 ttccattaaa caatggtact tgaactacat ctgcagtttc aatttggctt gcaactgcct
     8221 cttcaacata tctattagct tgataacttg aaataattcc atgaaactca tccatggcta
     8281 ctaatacttt cataaccttg accaccttac tttattattt gttgtgatac gttttttatt
     8341 cataaaagat taatcttaaa ttttagattt tatctcgtga ttatatgcga acatttaaca
     8401 tgacatgatt attaattatt ccagtaaata acaatatgct tcttttggtt tagcatatat
     8461 ttcctattaa actaacttac aaatcatgtt taaattttct tattggattc gtcgttttcc
     8521 aaaatatttt caataaaaat actcgaatat attatacata tgtttgtaaa aaagtactaa
     8581 cttaactgct tctaaaggtg acataaaata aaaggtcaga acatcattat agccaaacta
     8641 ctctgattca cacgaattta agttgtgcta ttcacgtcta gtagtgtaag gcaaaatgtt
     8701 ccaaccctat agtttcatta ttcttctgct tgtgctaatg acgacacatt gatatcaccg
     8761 tggtctcgat tccaaaccat ttcaccattt acaaaataaa atgcttgagg tgattcatgt
     8821 ttaacgttcg tttttttagc aatataatct gacaaatcgc gttcttgttg gacaatcaaa
     8881 taataaccgt ccatatcgcg ttcatataaa aatttattaa attgatcgta cgcatttgcc
     8941 gatattggac aagtttcact atgttttaat acaaaaacat atttattttc ctcaataacc
     9001 tgttcaaatt ggtcaattga acttagcttt atagccactc tattcacctc taaaaatatt
     9061 tatcacattt aaatagcatc tgcgtgacat ttttatacat atagttaata tcaatgttta
     9121 ccattataca catagtaagt tgacatgtta accataaatt caaaattttt aactttgaaa
     9181 aatgattata gttatttttc ttctaatttt tcaagctttt tttcaaaggc tttatcgccg
     9241 tgagttatat ctataccctt tttcggcaac ttatctacat cgacttttga taacttctca
     9301 ctaaccttta ttgtttcaat acgtgtatta taatagttct gcagactgta atacatttct
     9361 attgcgtttt tccttgcatt attaacatgc ttatcactaa tattagtttg gttaatatcg
     9421 gtaatttgct tttcaatcat tggcataatg tgatttttaa tcgcgccttt tacttcattt
     9481 tctttactat catctaggtt cttcttcgca gtatctctta acgctttatt attatgttct
     9541 aattttgtcg taagattagt tgcttcactt ttattatcag ctaatttaat ttctgattca
     9601 actttgtatc tatttttttc aaattgtttc gtataatcta aaatgtcttc gttatacttg
     9661 atagattgat tacataaacc tatgaatttt tttaattgat atatcgcatc cttcttctca
     9721 tttgcaagcg tcatatattc tttttttaat ttcttaactt tcattgtatc atcaggcaaa
     9781 ttttttgctg acttataata tgcttcaaat ttaggtatga gatggttttt aacatcttct
     9841 tgtagtgcct taaattcttt actattttta tcagttgtat cagttttact tagatgatca
     9901 atttctttca aatgtatatt atccatgaca tgttgtaatt ctttttgttt ttgctcaaca
     9961 tctttaaatt gcttagtaaa ttgatgttgt gcctcttttt ctttattacc acaagcactt
    10021 attactacaa ataaaatcgc taaaacagcg ataataacaa tttttttcat gattgcactc
    10081 cttaacagat tcattataca aaaacatgtt tattatgaaa atgaattatg ttatagtatt
    10141 ccagacttta tcgtgattaa attaattttc aatatcaaag gagtttatca tgtattcaaa
    10201 tgttcaacca ttcattaata gtgatttaac taattgtttt caatcacaat acactgaaat
    10261 tgaatcatta ttattcaacc tcttagacag cccagtaaaa tatacaaaac atattggcgg
    10321 tacgcatcat tttaaatatt caacagagcc aattttagat attttagtag gtgtagaaaa
    10381 tttgcatgat attacatcct tagatgaaaa acgcttaaat tatgtagggt tttatcgttt
    10441 gcatcataaa tatactaaga aagttatgat ggcgaagttt aacaattttc atgaactaaa
    10501 acaacaggtg cgtctacata tcattcaaat gaatacccaa acatttgaat cttatcttgc
    10561 acttgattac ctcttatcaa ctaatgatga tattgctttg caattcaata caaaaaaaca
    10621 ccaaatattg caacatagct taacaattag acaatatgag atgcaaaagc aacaatactt
    10681 tgaaaaacta tgtaaaaaat acttataacg acaataatgc cgtattttat aattatacta
    10741 attctaatac tttcttttca attttcgcaa atgaatttta aaattggtat aatactatat
    10801 gatattaaag acatgagaaa ggatgtactg agaagtgata aataaagaca tctatcaagc
    10861 tttacaacaa cttatcccaa atgaaaaaat taaagttgat gaacctttaa aacgatacac
    10921 ttatactaaa acaggtggta atgccgactt ttacattacc cctactaaaa atgaagaagt
    10981 acaagcagtt gttaaatatg cctatcaaaa tgagattcct gttacatatt taggaaatgg
    11041 ctcaaatatt attatccgtg aaggtggtat tcgcggtatt gtaattagtt tattatcact
    11101 agatcatatc gaagtatctg atgatgcgat aatagccggt agcggcgctg caattattga
    11161 tgtctcacgt gttgctcgtg attacgcact tactggcctt gaatttgcat gtggtattcc
    11221 aggttcaatt ggtggtgcag tgtatatgaa tgctggcgct tatggtggcg aagttaaaga
    11281 ttgtatagac tatgcgcttt gcgtaaacga acaaggctcg ttaattaaac ttacaacaaa
    11341 agaattagag ttagattatc gtaatagcat tattcaaaaa gaacacttag ttgtattaga
    11401 agctgcattt actttagctc ctggtaaaat gactgaaata caagctaaaa tggatgattt
    11461 aacagaacgt agagaatcta aacaaccttt agagtatcct tcatgtggta gtgtattcca
    11521 aagaccgcct ggtcattttg caggtaaatt gatacaagat tctaatttgc aaggtcaccg
    11581 tattggcggc gttgaagttt caaccaaaca cgctggtttt atggtaaatg tagacaatgg
    11641 aactgctaca gattatgaaa accttattca ttatgtacaa aagaccgtca aagaaaaatt
    11701 tggcattgaa ttaaatcgtg aagttcgcat tattggtgaa catccaaagg aatcgtaagt
    11761 taaggagctt tgtctatgcc taaagtttat ggttcattaa tcgatactga aacacgctgt
    11821 cgccattatt ttaccgaaga agatattatt gctattaaat ttaaatgttg taataaatac
    11881 tatccatgct ataagtgcca taatgagttt gaaaagcacg ccattaagcg ttggtctgag
    11941 ccttcattta acgaaaaagc aatcttatgc ggtgtatgca aacacgaatt aacaatcaat
    12001 gaatatatga tggtagagcg ttgtccaaat tgccaatctc ggtttaacaa tcgctgtaaa
    12061 tatcactatc atatttattt tgaaatttaa acaacatagt caaaacatta tagggagtgg
    12121 gtcagacatg ataaagcacc acttctgtag aattcctttt tgaaattctc tatgtttggg
    12181 ccccgccaac ttgcattgtc agtagaaatt gaaaatcaaa tttctctatg tttggagccc
    12241 actagtattg aaatgaactt gttacaagta catgttcatt caaccaacga ctgacaacag
    12301 aacattttag agtctaggac attgattgac gtcccagatt tcctttttta aagaaaagag
    12361 gggagcaatg attaagacct ttaaccattg ttcccctctt ttaaaattat tttacaactt
    12421 tatcaagttc ctcaatttgt ttaattgtag ttgtagttga tccagctgca aagtaccata
    12481 atttaggatc taaattatat actttgtctt ctttaattgc tttaacattt tttaatacag
    12541 gattatttaa tgcttgtttc gcagttgatt taccacttat cgcttgacct ctatccatcg
    12601 ctaaaataac atctggattt tctttattaa catattcgtt agaaacattt tgtccatgat
    12661 tgctattact tactttttta tcaactgcat tgaatcctaa tgtatcgtaa actaatccac
    12721 caaaacgacc tttaggtcca aatgttgata attcaccttc gttaactagt aaatacataa
    12781 cagttttatt gaagtttttc gttttatctt tcattgaagc aattttgtta tctaaatctt
    12841 tatttaattc tttagcttta tcttctttat cgtaaatttt tccgatattt tcagtgtttt
    12901 gtttcattga accaattaag ttcttttcat ctgcaccaac ataaacaatt ttcgctttag
    12961 gtgcagcttt tttgaattca tctaaattct tttgattagc tgtacgtcca gagataaaga
    13021 ttacttcggg tttcgtcgca gcaattttat caaaattcac ttcttttaaa ttaccaacgt
    13081 ttgtatattt atcatcttta aatgattcta agaaattcgg taatgactta ccgccttccc
    13141 ctttaggtaa tgcttttact ttatctgata agcccatttc tttcattaca tctaatgcgc
    13201 catagtctaa cacaactgca tttttaggat tttttggtac ttcaacagtt tctttaactt
    13261 ttttagcgtc actaccatct tttttctcgc cacgcatttt atagttattt tcaattttta
    13321 ctgtatcttt agaaccttta gtttctgatt ttgattgttc tttatcagaa ttgttaccgc
    13381 atgccgctaa taaaaacatt actgctaata ctaaatataa gactgttttc ttcataatat
    13441 aatattctcc tcttttggct taatctgtgt gcttgatgtg aaatataaat aaatgcttta
    13501 cgtttaaccc ttctttaatt ggcgcatcac ccctttaaag tgtctccaaa ttatcaatcc
    13561 ttaaaatata aacaaatcct ttgatttctt atatgttcaa tttgaatatt catgtcatat
    13621 aaatcactta aaacactact ttgaatgaca ttatctttca aatctgactt aactagttgt
    13681 ccgtttttca atgctacaat ctgatctgaa taacaggacg caaagttaat atcatgtaac
    13741 acaatgacaa tcgctttatt cattttatgc gctaacattt ttaacgtttg cataatttga
    13801 acagcatgct tcatatctaa attatttaat ggttcatcta gcaaaatata ttcagtatct
    13861 tgtgctattg tcattgcaat gtatgcacgc tgacgttgtc caccagataa tgacttaata
    13921 ttacgatttc tgatatcttg tagttgcaac aaatctagcg catcattgac aatatcatga
    13981 tcctctttcg tcaaacgacc tttagaataa gggaatcgtc caaagtttac caactgctct
    14041 accgtaatat tcatttcagt atggtttgtt tgttttaata tagatatttt tttcgacaag
    14101 tcattatttt tataatcaga catgagctgt ccatctattt tcacttcacc gttctcaaaa
    14161 cgaattaacc tacatatcgc tgaaagtaaa gtactcttac ccgcaccatt aggtccaatt
    14221 aaagaagtca atttaccttt ttcgatatct atgctaatat cttccaatat catttgatta
    14281 tttatagttt tagttaaatt ttcaacttga atcattgcgc atttctcctt ctaactaata
    14341 gataaatgaa ataacttcca ccaatcaaat caataagtat actcatttct gtcgtagctt
    14401 caaacacatt ttcaactacc cattgcgcac taaataaact aatccaactc aagcaaattg
    14461 tcgcaattaa aatatactta tgttcatacg tcttcattag ttcatgcgct agatttacag
    14521 ttaataaacc taaaaatgta attggtccta ctaatgcagt tgacacagaa actaaaactg
    14581 agactattac aagtagaatt cgcgttaatt tttcatacga tatcccaaga ttaattgctt
    14641 caacacgacc taaaagcaat acatctaaat aaggcaatag taaaattgta atgactaata
    14701 agattactaa tagcactgct gagaaagtaa ctaaattcga attagaagca ttaaaattag
    14761 caaacatact actttgtatt gctaaaaatg actcaggatc cataatcagt tgaataaaac
    14821 ctgttatgct tctaaaaaat gttcctaaaa ggacaccgat aagtaagata aaataaattg
    14881 aaaaatgtcc gattttaaag ataccttgga ataggattag tgcgaataac accatcgtta
    14941 taagtgttaa tataaagttc aaatatacat tagtaaccca taccgattga attccaaata
    15001 aaaagactgg caatactttg ataaataaat aaactgcatc taaccccatt attgatggtg
    15061 tcaatagacg gttatttgta atcgcttgaa aaatcaccac tgaagttgca atggcagcac
    15121 ctactaaaat aattaatatg aactttctta aacgacttga aaattgatat tcaaatattt
    15181 caaaatcaat acctacaaat aagtacagca tactaattaa taacgttact gcaattaaaa
    15241 gcattatttt tttattattg ttgcgcataa tttttcctac ctttcataag caagataagg
    15301 aaaatgattg ttccaaatac acctattgtt aaaccaatat ttatttcata tggataaaca
    15361 acaattctgc caactatatc agaaaataat acaaagatgg cacctaacat catcgtatga
    15421 gggatagcat ttttcaaatg atcacctcga taaattgaaa taatatttgg tattactagt
    15481 cctaagaacg gtaatgttcc aacagtcacc actaccaatg ctgtaatagt tgcagtaata
    15541 aacaatgcga tgttaattaa tttttcgtaa ctcaaaccta aattattagt aaagtcttta
    15601 cccattccta caatcgtgaa atgattagca aaaagatatg tcaatgctaa aagaggaata
    15661 cttaaatata aaatttcata gcgtccactt gtgataatgg caaagttccc gttaagccag
    15721 ttaccaatgc tttgaacagc attcgtgcgc aatgagatga aggttgtgaa acttgcaaca
    15781 atcccaccca tcataattcc taaaagcggt acaaaaatga catctttcac ttttataaaa
    15841 tcaatgattt taacaaataa aaacgtaccg caaatactgc aaataacagc aaatactagt
    15901 tttaataaaa tatgaccggt tggaaagaac aataaagcaa ttaaaatacc tagtttagcc
    15961 cattccatcg ttccagctgt agtcggacta acaaacttat tttgcatcat ttgttgcatt
    16021 atcaagcctg ctaaagccaa cgaacttcca gcaattaaaa tactcatcgt ccttggtatt
    16081 cggcttgaga ataaaatatt ccgctgtgac tcagttaaat gtagtaaacc tttaattgat
    16141 agttcactca cacctatgaa caacgaaata attgtcaaca atattagtaa tagaagcaaa
    16201 atatttccct ttaataaata cttcattaca aatttttctc cctttaaatt aatgataatc
    16261 attatcattg ataatatttc tcattaatag tagcgttttt tgaccataat ttcaatagcc
    16321 tttcaaataa tttagtgtca attttattga tatttgcaat taaactgaaa tttaattttt
    16381 cggaatgtgt attttgttta cttaaagtaa aatagaacac ttaagatttt gatgtctggg
    16441 aatagtggaa atgataaaaa ctactaatga ttgattaagt agtggttctt aacattagcc
    16501 tcagctaatg tgtacttaaa aataggaata catgagtaaa actcatgtgg aagtgataat
    16561 ttctaaagga aaaagttttt ctttatgttg gggccccgcc cgaacttgca caatattgtt
    16621 agctgacttt ccgcctgctt ctgtgttggg gccccgccaa ctactgccaa tataaatagc
    16681 attgtacatc ttagagcatt gatttcttac tcagactcat aatgagttag atacacataa
    16741 aaaaagacct tcaacatgta ggagacaaga cgaaacgaat cgctatttcc ctttacatgt
    16801 gaaggtcttt taatttaatt gttatttgtt gtcaaataca aagtcatcat cttgtaatgc
    16861 ttctacgttt aatgctaata cataaccatc accttttact gagaagaagt catggttttt
    16921 agttgttgta tctaaggcat tctcaatgat tgggttaaat tcacgttcct caaaataagg
    16981 ttcaaagcct aagtttgaaa gtgctttgtt tccattatat ttaacatagt ttagcacatc
    17041 ttcagtgatt ccaagatcat cgtataacat ttttgtgtat gactcttcat ttaaatacaa
    17101 gtcatttagc aatttataca tttcttgatc tgctttttgt ttctcacttt cagatagttc
    17161 atttcgtaaa tgctgtgcat ctaaaccggt aaatacacca tgaatagatt catctaaaag
    17221 aattttacga atgatttcac ctgatgtcgt cattttccct tgaccagcaa gatatagtgg
    17281 ataatagaaa cctgagaaga ataagaatgt ttctaaaaat acactcgtaa ctctggccat
    17341 atattggtcg tatatcgaag cttctttacc ccaaagtttg tgataattag caacaatttt
    17401 atcagattta tattttaaat ggggttcctc taaaacccat tcatctaata ggtagtttgt
    17461 ttcactagat ggtaatagtg ttgtgaaaat atgtgaatag cttttcgcgt gtatttgctc
    17521 catcatcgcc ataaatgaat aaactgcttt tttccttaag tcagtcgtat gtagcataac
    17581 taaaggcatg ccatcatctg cttgatgtgt atctaagcct gttaaaccag ctaatgcttt
    17641 tttaaatgtg tcttgttcag cttcagataa agtcttccaa cttgcaatgt cttttgatac
    17701 tttaaattct gtttcaaccc acatttgaga tatattttgt ctccaaaaca tattcgtcat
    17761 atcttcttgt gtgttccaat taacagctat catgaatata atttcctcct gtaatgttac
    17821 tcaataattg acaaatacag acatagcagt ctaatcatct gctaattcag accagatgat
    17881 tagctgtttg tcattttcaa catttaattg ttagatagaa caacttgtac attcttctac
    17941 acttaataat ttatttctag tatagtaaag tgattttaat cctttatagt gcgcatatac
    18001 atataatctt gctaactcac gtgtagaaat ttcagaatta acataaagga tcgttgagat
    18061 accttggtca atatgcgttt gaattgtcgc aattaaatcg attaatttca tctgatcagt
    18121 attgaatgct gatttgtagt accacattgt ttgtggtgat aagaatggca tagggtaaaa
    18181 tgtttccgca ttaccataag tacgacgttc aatttggtca acgattggca ttacagaact
    18241 tgttgcattt tgaacataag aaatactttg tgttggagca attgctaatc tatatgcatg
    18301 atataaacca tattgttcaa catcttgttg tagtttcttc caatcctcag aagtaggaat
    18361 agccatacca tcgaataatt cacgtacttt ttcgaattga ggttcaaatt cttgagttgt
    18421 atagaactcg aaatatttgc cattagcata atcagacttt tcaaagtctt gatatttgat
    18481 accacgctct ttagcgattt ccattgaacg ttcgattgag tagaaattca tcatcataaa
    18541 gaagatattt gcaaaatctt ttgcttcttc tgactcataa ccaattttat tttttgctag
    18601 gtaaccgtgt aaattcatca caccaagacc aactgaatgt aattcactgt tagcttttct
    18661 aactcctggt gcattttgaa tatttgctac atcactcaca acagttaatg cgtccatacc
    18721 agagtgaact gaatctctga attttccgct ttccattaca ttaacaatat ttaatgagcc
    18781 caagttacaa gaaatatcac gtttaatttc gtcttcaata ccatagtcat taataattga
    18841 agtttcttgt agttggaaaa tttccgtaca taagttactc attttaattt gtccaatgtt
    18901 tgaattcgga tgcactctgt tagcattatc tttaaacatt aaatatggat aacctgattg
    18961 taattgtgtt tgcgcaatta aattcaacat ttcacgcgca ttctttttct ttttctcaac
    19021 atttgggttt gcaaccatgt catcataata tttttctaaa tcgatatcgt ctaatgtcac
    19081 accatattct tctttaactg tatgaggtgc aaacatataa aagtccttac cttctttagc
    19141 taaatcgaag aatttagatg gaacaattaa accagttgat attgtagata aacgtaaatc
    19201 ttcatccgca tttacttttt tagtatctaa aaattcttct acatcataat ggaagatatt
    19261 taagtacaca gcaccagcac cagggcgttg accaagttga tctgcatagc taaagccacc
    19321 ttcaagtgac ttagcaatag gtaaaacgcc tttcgctacg cctttaattc ctttaattgc
    19381 ttcaccacgt gcacgcaatt tagataagtt aattgcaacg ccgcccccaa ttttacttaa
    19441 ttgttttgca gttgaatcaa taaagttaat tgaatttaag ctgtcatcca cttctaataa
    19501 gaaacatgac actagctcac cacgacgcgc acggcctgcg tttaaaaatg ttggtgtcgc
    19561 tggttgatat ctttgttcaa ccatagcaga aataaattgt ttagcttgtg ctttattacc
    19621 atttgctagg tataaagcaa caatggcaac gtgttgatta tagtcttcta agtattgact
    19681 tttatcattt gttttcaaag cgtaatcttt gaaaaattta ctagctgaca tataacttgc
    19741 aaaattaaac gggattgatt ttgcataatc agtgatttca attagatccg cttcactata
    19801 aatatcaaac acattgaaat aaaaatcgtt gtctactaaa taacgtaaac gctcgatttc
    19861 agtgtcgaag aagattgttt tgtcttttac ttcttctaaa taagctacta aagcttcttg
    19921 gtctttttct aaactaaaga atccatcttc tcttcgttta gtgacctcat tatttaattc
    19981 aatatgattg tactttttct cttccatggt tttcattaaa attacccacc ttgttcttaa
    20041 attcaataac gtctttgttt tttccatgta actcaaactt cattaataaa gggacattat
    20101 actcttctga tatcgtgcga cccgcttttg cgaaatttag tccccaattt cgattaccgc
    20161 tagctgccac acctctgatg tattgatgat taacttctaa aaaagattga acgggttctg
    20221 gtacttctcc aaatccaata gtgccagtaa cgataataaa cggttcatga actggttcca
    20281 tacaattttc tgctgtaatc tcaagcgtat tttcaagttc tgttctctta ataaaacgac
    20341 ggacatttcc agtaaatgaa aaatatatta ttttcattgg atcaccttct ttcttcaacc
    20401 cttttaaata aagtagtcat tccaattaaa aaatgcttta acaacatggt tactatggtt
    20461 atctcagcat tgtattaact ataatgaagc tacttatttt ctcaaatgta aaccaattgc
    20521 atagatttat atttgagaaa atagtagctt atatgggttg aatgtataaa aatcactata
    20581 tgtagtgttt acatcattgg atatcttcca tttgctcagt acattttcta aaaatatgac
    20641 tattaattga taataactat taaatttctg tgttaaatta cttttaaatg taatttcttc
    20701 ataaataaag catttgcata caaaacacaa gatattgtgt ctttttcgtt tcaccaacac
    20761 tatattatgt gtttcattat acataagaca cactatgatt taaagtgcca ttttctaatc
    20821 gaatgttcgt aaaacgttcc aactaggcac caatactaaa ttagaaaaac ttgaacaaat
    20881 ttaaaaaaat tttttagtag atatcacttg atttttttaa atctgtgtca atctcaattg
    20941 tatcaacaga aatcaagaaa aagcttatta gcttgatact gaattgatat ttgttttatg
    21001 taataacaca aacattcata aaagtgtaat gccctattat tccgacaaat aaacacagaa
    21061 cgtttgttcg ctacagtcta ttatagtctt tttatagtaa atttcaacta tttatgcaca
    21121 acatgttgtt ttatgctaat ataaatacgt taattttata taaaagaggc gataaaattg
    21181 aatcctaaag taaaaggtat cattgccatt ttaatttcgg ctattggttt cagttttatg
    21241 tcagtgtttt tcagacttgc aggtgacctt cctgtttttc aaaaatcatt agcacgaaac
    21301 ttagtagcaa tgtttattcc attatttttt atatataaat accatcagcc aatgtttggc
    21361 agactttcaa gtcaaccctt attaataaca cgatcaacac ttggacttat tggtgtattg
    21421 ttaaatattt atgccattga tcatatggta cttagtgatg ccgattcatt aatgaaactc
    21481 aatcctttct ggacgatttt attaagcatt gttttcttac atgaaaaggt aaggaagtat
    21541 caaattacag caatgataat tgctatttta ggtatgcttt taatagtgaa acctgaattt
    21601 tcatcatcta tgattccatc attagctggc ttattctcag gtatttttgc agcatcagca
    21661 tatacatgtg ttcgagcact tagtacaaga gaagcaccat atactatagt attttacttc
    21721 tccttatttt ctgtcatcgt tttaatacct ttcactgcat atacttatga accaatgagt
    21781 cagatgcaaa tactttatct attaggggct ggcttagctg ccgctgtggg tcaaattggt
    21841 gttactttag catatagttt tgcagcagca aaagatattt ctatttttac gtatgcatct
    21901 ataattttca cagcaatact tggatttatt ctattcggag aatctccaga tttctatgca
    21961 acattaggtt acgtcgttat tatcggtgca agttattaca tgtttgaaaa agcacgccga
    22021 gacgccaaaa taataaaaaa ataaatttaa aggagcgctt tatatggcac atggccgtca
    22081 acaagatgaa ttacaagata taactttatt aggaaatcaa gacaatactt acaattttga
    22141 ctatcgtcct gatgtattag aatcgtttga taataagcat caaggtcgtg actatttcgt
    22201 aaaattcaac tgtccagaat ttacatcatt atgtccaatt actgggcaac cagattttgc
    22261 aactatctat atttcatata ttccaaatgt taaaatggtt gaatcaaaat ctttgaaatt
    22321 atacttattt agtttcagaa atcacggtga ttttcacgaa gattgtatga atattattat
    22381 gaatgatttg atagagctta tggacccaca ttatattgaa gtctggggca agttcacacc
    22441 acgtggtgga atttctattg atccttatac aaactatgga cgtccaaatt ctaaatatga
    22501 aaaaatggct gagcatcgtt tgatgaatca tgatttatat cccgaaaaaa tagataatcg
    22561 ttaaatgtat catttaataa acacaccaat aagttgattt tcctaactta ttggtgtgtt
    22621 tttcatttag catacataat aggttacatt aaaataacat tttataccaa agtacaccaa
    22681 aagaatatta gtacacgaat taaacaacat ttttatagaa acctattgca ctttaacgtc
    22741 aataagtata tttttatatt atctctaatt aattgtgcgc gcttaataac agaatattct
    22801 caatattttt atttttttgt gatttgttgg aatatttagt tgataaggca caatcaaatt
    22861 tacttaaact attgtattag gggaagaaag gatgggatgt atacatgaca caacaaaact
    22921 cccatggaaa tcaaattcaa gacatacctc aaacaggatt tttcgggcat cctcgaggac
    22981 taggcgtact cttctttgta gagttctggg aaaggtttag ttattatggc atgcgtgccc
    23041 tactcatttt ctacatgtac tttgccgtaa cagataatgg ccttggaatt gataaaacaa
    23101 cagctatgtc aattatgtca gtttatggtt cattaatcta tatgacatca ataccaggcg
    23161 gatggattgc tgacagaatt acaggcacta gaggcgctac tttattaggt gcagtcttta
    23221 ttattatcgg acatatttgt ttaagcttac catttgcatt aatcggctta ttcacatcaa
    23281 tgttcttcat cattattggt tcaggtttaa tgaagccaaa catttcaaat atcgttggcc
    23341 gtttatatcc tgaaaatgat agacgtatgg atgcaggttt tgttattttc tatatgtcag
    23401 ttaatatggg tgcattatta tcacctatta ttttgcaaca ctttgttaat gttaaaaact
    23461 tccacggcgg attcttgatt gcagcagttg gtatggcatt aggtttagta tggtatgtac
    23521 ttttcaaccg caaaaactta ggtagcgttg gtatgaaacc tactaaccca ttgacaccag
    23581 ctgaaaagaa aaagtatggt cttattatcg gaagtgttgt tttagcaatt gtattaatta
    23641 tcgttattgg tgcattaact aactcattat catttaactt agttagtaat actgttttag
    23701 tattaggtat tgcattacca atcatttact tcactttaat tattagaagt aaagatgtca
    23761 ctgatactga acgttctcgt gttaaagcat ttattccatt atttattctt ggaatggtgt
    23821 tctgggctat tcaagaacaa gggtctaatg tattaaacat atatggaatt gaacattcag
    23881 atatgaaatt aaacttattt ggttggaaaa caaactttgg tgaagcgatc ttccaatcaa
    23941 ttaacccatt atttatttta ttactagctc caattatttc acttttatgg caaaagcttg
    24001 gaactaaaca acctagcctg ccagtaaaat ttgcaattgg tacgttctta gcaggtgcgt
    24061 catacatact aattggtatt gtaggttatg catcaggttc atcaaacttt tcagttaact
    24121 gggttatttt atcgtacatt atttgtgtta tcggtgagct atgcttatca ccaacgggta
    24181 atagtgctgc tgttaagtta gcacctaaag catttaatgc ccaaatgatg agtatttggt
    24241 acttaactaa cgcttctgca caagcaatca atggtacttt agttaagtta atagaaccac
    24301 taggtcaaac aaattacttt attttcctag gtgttgttgc aattattgtt acaacaattg
    24361 tattagcatt ctcaccttta atcatcaaag cgatgaaagg tatacgttaa tattgttggc
    24421 ctaattcaaa aaacagtaag tcatttaaat ggcttactgt ttttttatag gtttctatta
    24481 attaaattca agatatcagt ataaataaaa acttaatatg ctca
//
LOCUS       DAXWFK010000022        23302 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.022, whole genome shotgun sequence.
ACCESSION   DAXWFK010000022 DAXWFK010000000
VERSION     DAXWFK010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 23302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23302
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.022"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            86..2161
                     /gene="topA"
                     /locus_tag="ACGEXS_000702"
     CDS             86..2161
                     /gene="topA"
                     /locus_tag="ACGEXS_000702"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFF8011740.1"
                     /translation="MTLADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQM
                     GVDTEDNYEPKYITIRGKGPVVKELKKHAKKAKNVFLASDPDREGEAIAWHLSKILEL
                     EDSKENRVVFNEITKDAVKESFKNPREIEMNLVDAQQARRILDRLVGYNISPVLWKKV
                     KKGLSAGRVQSVALRLVIDRENEIRNFKPEEYWTIEGEFRYKKSKFNAKFLHYKNKPF
                     KLKTKKDVEKITAALDGDQFEITNVTKKEKTRNPANPFTTSTLQQEAARKLNFKARKT
                     MMVAQQLYEGIDLKKQGTIGLITYMRTDSTRISDTAKVEAKQYITDKYGESYTSKRKA
                     SGKQGDQDAHEAIRPSSTMRTPDDMKSFLTKDQYRLYKLIWERFVASQMAPAILDTVS
                     LDITQGDIKFRANGQTIKFKGFMTLYVETKDDSDSEKENKLPKLEQGDKVTATQIEPA
                     QHYTQPPPRYTEARLVKTLEELKIGRPSTYAPTIDTIQKRNYVKLESKRFVPTELGEI
                     VHEQVKEYFPEIIDVEFTVNMETLLDKIAEGDITWRKVIDGFFSSFKQDVERAEEEME
                     KIEIKDEPAGEDCEICGSPMVIKMGRYGKFMACSNFPDCRNTKAIVKSIGVKCPKCND
                     GDVVERKSKKNRVFYGCSKYPECDFISWDKPIGRDCPKCNQYLVENKKGKTTQVICSN
                     CDYKEAAQK"
     gene            2317..3624
                     /gene="trmFO"
                     /locus_tag="ACGEXS_000703"
     CDS             2317..3624
                     /gene="trmFO"
                     /locus_tag="ACGEXS_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HFF8011741.1"
                     /translation="MTQTVNVIGAGLAGSEAAYQLAERGIKVNLIEMRPVKQTPAHHT
                     DKFAELVCSNSLRGNALTNGVGVLKEEMRRLNSIIIEAADKARVPAGGALAVDRHDFS
                     GYITETLKNHENITVINEEINAIPDGYTIIATGPLTTETLAQEIVDITGKDQLYFYDA
                     AAPIIEKESIDMDKVYLKSRYDKGEAAYLNCPMTEDEFNRFYDAVLEAEVAPVNSFEK
                     EKYFEGCMPFEVMAERGRKTLLFGPMKPVGLEDPKTGKRPYAVVQLRQDDAAGTLYNI
                     VGFQTHLKWGAQKEVIKLIPGLENVDIVRYGVMHRNTFINSPDVLNEKYELISQPNIQ
                     FAGQMTGVEGYVESAASGLVAGINLAHKILGKGEVVFPRETMIGSMAYYISHAKNNKN
                     FQPMNANFGLLPSLETRIKDKKERYEAQANRALDYLENFKKTL"
     gene            4041..4937
                     /gene="xerC"
                     /locus_tag="ACGEXS_000704"
     CDS             4041..4937
                     /gene="xerC"
                     /locus_tag="ACGEXS_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFF8011742.1"
                     /translation="MNHIQDAFLNTLKVERNFSEHTLKSYQDDLIQFNQFLEQEHLEL
                     NTFEYRDARNYLSYLYSNHLKRTSVSRKISTLRTFYEYWMTLDENIINPFVQLVHPKK
                     EKYLPQFFYEEEMEALFKTVEEDTSKNLRDRVILELLYATGIRVSELVNIKKQDIDFY
                     ANGVTVLGKGSKERFVPFGAYCRQSIENYLEHFKPIQSCNHDFLIVNMKGEAITERGV
                     RYVLNDIVKRTAGVSEIHPHKLRHTFATHLLNQGADLRTVQSLLGHVNLSTTGKYTHV
                     SNQQLRKVYLNAHPRAKKENET"
     gene            4934..5479
                     /gene="hslV"
                     /locus_tag="ACGEXS_000705"
     CDS             4934..5479
                     /gene="hslV"
                     /locus_tag="ACGEXS_000705"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFF8011743.1"
                     /translation="MSNTTLHATTIYAVRHNGKAAMAGDGQVTLGQQVIMKQTARKVR
                     RLYEGKVLAGFAGSVADAFTLFEKFETKLQQFSGNLERAAVELAQEWRGDKQLRQLEA
                     MLIVMDKDAILVVSGTGEVIAPDDDLIAIGSGGNYALSAGRALKRHASHLSAEEMAYE
                     SLKVAADICVFTNDNIVVETL"
     gene            5545..6948
                     /gene="hslU"
                     /locus_tag="ACGEXS_000706"
     CDS             5545..6948
                     /gene="hslU"
                     /locus_tag="ACGEXS_000706"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFF8011744.1"
                     /translation="MDTAGIRLTPKEIVSKLNEYIVGQNDAKRKVAIALRNRYRRSLL
                     DEESKQEISPKNILMIGPTGVGKTEIARRMAKVVGAPFIKVEATKFTEVGYVGRDVES
                     MVRDLVDVSVRLVKAQKKSLVQDEATAKANEKLVKLLVPSMKKKASQTNNPLESLFGG
                     AIPNFGQNNEDEEEPPTEEIKTKRSEIKRQLEEGKLEKEKVRIKVEQDPGALGMLGTN
                     QNQQMQEMMNQLMPKKKVEREVAVETARKILADSYADELIDQESANQEALELAEQMGI
                     IFIDEIDKVATNNHNSGQDVSRQGVQRDILPILEGSVIQTKYGTVNTEHMLFIGAGAF
                     HVSKPSDLIPELQGRFPIRVELDSLSVEDFVRILTEPKLSLIKQYEALLQTEEVTVNF
                     TDEAITRLAEIAYQVNQDTDNIGARRLHTILEKMLEDLSFEAPSMPNAVVDITPQYVD
                     DKLKSISTNKDLSAFIL"
     gene            6973..7746
                     /gene="codY"
                     /locus_tag="ACGEXS_000707"
     CDS             6973..7746
                     /gene="codY"
                     /locus_tag="ACGEXS_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFF8011745.1"
                     /translation="MSLLSKTRELNTLLQKHKGIAVDFKDVAQTISSVTVTNVFIVSR
                     RGKILGSSLNELLKSQRIIQMLEERHIPSEYTERLMEVKQTESNIDIDNVLTVFPPEN
                     RELFIDSRTTIFPILGGGERLGTLVLGRVHDDFNENDLVLGEYAATVIGMEILREKHS
                     EVEKEARDKAAITMAINSLSYSEKEAIEHIFEELGGTEGLLIASKVADRVGITRSVIV
                     NALRKLESAGVIESRSLGMKGTFIKVKKEKFLDELEKSK"
     gene            7797..7889
                     /locus_tag="ACGEXS_000708"
     CDS             7797..7889
                     /locus_tag="ACGEXS_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011746.1"
                     /translation="MAVVNEMTLMIMLKSFLYFCLIFNQIDISH"
     gene            8088..8855
                     /gene="rpsB"
                     /locus_tag="ACGEXS_000709"
     CDS             8088..8855
                     /gene="rpsB"
                     /locus_tag="ACGEXS_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFF8011747.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKKVDEAYNFLKQVSEDGGQVLFVGTKKQAQESVKSEAERAGQFYINQRWLGGLLT
                     NYKTISKRIKRISEIEKMEEDGLFEVLPKKEVVELKKEYDRLIKFLGGIRDMKSMPQA
                     LFVVDPRKERNAIAEARKLNIPIVGIVDTNCDPDEIDYVIPANDDAIRAVKLLTAKMA
                     DAILEGQQGVSNEEVAAEQNIDLDEKEKSEETEATEE"
     gene            8889..9002
                     /locus_tag="ACGEXS_000710"
     CDS             8889..9002
                     /locus_tag="ACGEXS_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011748.1"
                     /translation="MGDKILMLITFFKKKIEANYKYSIRVLAILPIIMLKS"
     gene            9037..9918
                     /gene="tsf"
                     /locus_tag="ACGEXS_000711"
     CDS             9037..9918
                     /gene="tsf"
                     /locus_tag="ACGEXS_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFF8011749.1"
                     /translation="MATISAKLVKELREKTGAGMMDCKKALTETDGDIDKAIDYLREK
                     GIAKAAKKADRIAAEGLVHVETKGNDAVIVEINSETDFVARNEGFQELVKEIANQVLD
                     TKAETVEALMETTLPNGKSVDERIKEAISTIGEKLSVRRFAIRTKTDNDAFGAYLHMG
                     GRIGVLTVVEGSTDEEAARDVAMHIAAINPKYVSSEQVSEEEINHEREVLKQQALNEG
                     KPENIVEKMVEGRLRKYLQEICAVDQDFVKNPDVTVEAFLKTKGGKLVDFVRYEVGEG
                     MEKREENFADEVKGQMK"
     gene            10054..10776
                     /gene="pyrH"
                     /locus_tag="ACGEXS_000712"
     CDS             10054..10776
                     /gene="pyrH"
                     /locus_tag="ACGEXS_000712"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFF8011750.1"
                     /translation="MAQISKYKRVVLKLSGEALAGEKGFGINPVIIKSVAEQVAEVAK
                     MDCEIAVIVGGGNIWRGKTGSDLGMDRGTADYMGMLATVMNALALQDSLEQLDCDTRV
                     LTSIEMKQVAEPYIRRRAIRHLEKKRVVIFAAGIGNPYFSTDTTAALRAAEVEADVIL
                     MGKNNVDGVYSADPKVNKDAVKYEHLTHIQMLQEGLQVMDSTASSFCMDNNIPLTVFS
                     IMEEGNIKRAVMGEKIGTLITK"
     gene            10795..11349
                     /gene="frr"
                     /locus_tag="ACGEXS_000713"
     CDS             10795..11349
                     /gene="frr"
                     /locus_tag="ACGEXS_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFF8011751.1"
                     /translation="MSDIINETKSRMQKSIESLSRELANISAGRANSNLLNGVTVDYY
                     GAPTPVQQLASINVPEARLLVISPYDKTSVADIEKAIIAANLGVNPTSDGEVIRIAVP
                     ALTEERRKERVKDVKKIGEEAKVSVRNIRRDMNDQLKKDEKNGDITEDELRSGTEDVQ
                     KATDNSIKEIDQMIADKEKDIMSV"
     gene            11722..12492
                     /locus_tag="ACGEXS_000714"
     CDS             11722..12492
                     /locus_tag="ACGEXS_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFF8011752.1"
                     /translation="MFKKLINKKNTINNYNEELDSSNIPEHIAIIMDGNGRWAKKRKM
                     PRIKGHYEGMQTIKKITRVASDIGVKYLTLYAFSTENWSRPESEVNYIMNLPVNFLKT
                     FLPELIEKNVKVETIGFTDKLPKSTIEAINNAKEKTANNTGLKLIFAINYGGRAELVH
                     SIKNMFDELHQQGLNSDIIDETYINNHLMTKDYPDPELLIRTSGEQRISNFLIWQVSY
                     SEFIFNQKLWPDFDEDELIKCIKIYQSRQRRFGGLSEE"
     gene            12499..13281
                     /locus_tag="ACGEXS_000715"
     CDS             12499..13281
                     /locus_tag="ACGEXS_000715"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFF8011753.1"
                     /translation="MKVRTLTAIIALIVFLPILLKGGLVLMIFANILALIALKELLNM
                     NMIKFVSVPGLISAVGLIIIMLPQHAGPWVQVIQLKSLIAMSFIVLSYTVLSKNRFSF
                     MDAAFCLMSVAYVGIGFMFFYETRSEGLHYILYAFLIVWLTDTGAYLFGKMMGKHKLW
                     PVISPNKTIEGFIGGLFCSLIVPLAMLYFVDFNMNVWILLGVTLILSLFGQLGDLVES
                     GFKRHFGVKDSGRILPGHGGILDRFDSFMFVLPLLNILLIQS"
     gene            13518..14804
                     /gene="rseP"
                     /locus_tag="ACGEXS_000716"
     CDS             13518..14804
                     /gene="rseP"
                     /locus_tag="ACGEXS_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFF8011754.1"
                     /translation="MSYLVTIIAFIIVFGVLVTVHEYGHMFFAKRAGIMCPEFAIGMG
                     PKIFSFRKNETLYTIRLLPVGGYVRMAGDGLEEPPVEPGMNVKIKLNEENEITHIILD
                     DHHKFQQIEAIEVKKCDFKDDLFIEGITAYDNERHHFKIARKSFFVENGSLVQIAPRD
                     RQFAHKKPWPKFLTLFAGPLFNFILALVLFIGLAYYQGTPTSTVEQVADKYPAQQAGL
                     QKGDKIVQIGKYKISEFDDVDKALDKVKDNKTTVKFERDGKTKSVELTPKKTEKKLTK
                     VSSETKYVLGFQPASEHTLFKPIVFGFKSFLIGSTYIFTAVVGMLASIFTGGFSFDML
                     NGPVGIYHNVDSVVKAGIISLIGYTALLSVNLGIMNLIPIPALDGGRILFVIYEAIFR
                     KPVNKKAETTIIAIGAIFMVVIMILVTWNDIRRYFL"
     gene            14824..16527
                     /locus_tag="ACGEXS_000717"
     CDS             14824..16527
                     /locus_tag="ACGEXS_000717"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFF8011755.1"
                     /translation="MKQSKVFIPTMRDVPSEAEAQSHRLLLKSGLIKQSTSGIYSYLP
                     LATRVLNNITAIVRQEMERIDSVEILMPALQQAELWEESGRWGAYGPELMRLQDRHGR
                     QFALGPTHEELVTSIVRNELKSYKQLPMTLFQIQSKFRDEKRPRFGLLRGREFIMKDA
                     YSFHADEASLDQTYQDMYQAYSRIFERVGINARPVVADSGAIGGSHTHEFMALSAIGE
                     DTIVYSKESDYAANIEKAEVVYEPNHKHTTVQPLEKIETPNVKTAQELADFLGRPVDE
                     IVKTMIFKVDGEYIMVLVRGHHEINDIKLKSYFGTDNIELATQDEIVNLVGANPGSLG
                     PVIDKEIKIYADNFVQDLNNLVVGANEDGYHLINVNVGRDFNVDEYGDFRFILEGEKL
                     SDGSGVAHFAEGIEVGQVFKLGTKYSESMNATFLDNQGKAQSLIMGCYGIGISRTLSA
                     IVEQNHDDNGIVWPKSVTPFDLHLISINPKKDDQRELADALYAEFNTKFDVLYDDRQE
                     RAGVKFNDADLIGLPLRIVVGKRASEGIVEVKERLTGDSEEVHIDDLMTVITNKYDNL
                     K"
     gene            16791..21101
                     /locus_tag="ACGEXS_000718"
     CDS             16791..21101
                     /locus_tag="ACGEXS_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFF8011756.1"
                     /translation="MTEQQKFKVLADQIKISNQLDAEILNSGELTRIDVSNKNRTWEF
                     HITLPQFLAHEDYLLFINAIEQEFKDIANVTCRFTVTNGTNQDEHAIKYFGHCIDQTA
                     LSPKVKGQLKQKKLIMSGKVLKVMVSNDIERNHFDKACNGSLIKAFRNCGFDIDKIIF
                     ETNDNDQEQNLASLEAHIQEEDEQSARLATEKLEKMKAEKAKQQDNNESAVDKCQIGK
                     PIQIENIKPIESIIEEEFKVAIEGVIFDINLKELKSGRHIVEIKVTDYTDSLVLKMFT
                     RKNKDDLEHFKALSVGKWVRAQGRIEEDTFIRDLVMMMSDIEEIKKATKKDKAEEKRV
                     EFHLHTAMSQMDGIPNIGAYVKQAADWGHPAIAVTDHNVVQAFPDAHAAAEKHGIKMI
                     YGMEGMLVDDGVPIAYKPQDVVLKDATYVVFDVETTGLSNQYDKIIELAAVKVHNGEI
                     IDKFERFSNPHERLSETIINLTHITDDMLVDAPEIEEVLTEFKEWVGDAIFVAHNASF
                     DMGFIDTGYERLGFGPSTNGVIDTLELSRTINTEYGKHGLNFLAKKYGVELTQHHRAI
                     YDTEATAYIFIKMVQQMKELGVLNHNEINKKLSNEDAYKRARPSHVTLIVQNQQGLKN
                     LFKIVSASLVKYFYRTPRIPRSLLDEYREGLLVGTACDEGELFTAVMQKDQSQVEKIA
                     KYYDFIEIQPPALYQDLIDRELIRDTETLHEIYQRLIHAGDTAGIPVIATGNAHYLFE
                     HDGIARKILIASQPGNPLNRSTLPEAHFRTTDEMLNEFHFLGEEKAHEIVVKNTNELA
                     DRIERVVPIKDELYTPRMEGANEEIRELSYANARKLYGEDLPQIVIDRLEKELKSIIG
                     NGFAVIYLISQRLVKKSLDDGYLVGSRGSVGSSFVATMTEITEVNPLPPHYICPNCKT
                     SEFFNDGSVGSGFDLPDKTCETCGAPLIKEGQDIPFETFLGFKGDKVPDIDLNFSGEY
                     QPNAHNYTKVLFGEDKVFRAGTIGTVAEKTAFGYVKGYLNDQGIHKRGAEIDRLVKGC
                     TGVKRTTGQHPGGIIVVPDYMDIYDFTPIQYPADDQNSAWMTTHFDFHSIHDNVLKLD
                     ILGHDDPTMIRMLQDLSGIDPKTIPVDDKEVMQIFSTPESLGVTEDEILCKTGTFGVP
                     EFGTGFVRQMLEDTKPTTFSELVQISGLSHGTDVWLGNAQELIKTGICDLSSVIGCRD
                     DIMVYLMYAGLEPSMAFKIMESVRKGKGLTEEMIETMKENEVPDWYLDSCLKIKYMFP
                     KAHAAAYVLMAVRIAYFKVHHPLYYYASYFTIRASDFDLITMIKDKTSIRNTVKDMYS
                     RYMDLGKKEKDVLTVLEIMNEMAHRGYRMQPISLEKSQAFEFIIEGDTLIPPFISVPG
                     LGENVAKRIVEARDDGPFLSKEDLNKKAGLSQKIIEYLDELGSLPNLPDKAQLSIFDM
                     "
     gene            21391..21858
                     /gene="rimP"
                     /locus_tag="ACGEXS_000719"
     CDS             21391..21858
                     /gene="rimP"
                     /locus_tag="ACGEXS_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFF8011757.1"
                     /translation="MSKITEQVEVIVKPIMEDLNFELVDVEYVKEGRDHFLRISIDKE
                     GGVDLNDCTLASEKISEAMDANDPIPEMYYLDVASPGAERPIKKEQDFQNAITKPVFV
                     SLYVPIEGEKEWLGILQEVNNETIVVQVKIKARTKDIEIPRDKIAKARHAVMI"
     gene            21879..23054
                     /gene="nusA"
                     /locus_tag="ACGEXS_000720"
     CDS             21879..23054
                     /gene="nusA"
                     /locus_tag="ACGEXS_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFF8011758.1"
                     /translation="MSSNELLLATEYLEKEKKIPRAVLIDAIEAALITAYKKNYDSAR
                     NVRVELNMDQGTFKVIARKDVVEEVFDDRDEVDLSTALVKNPAYEIGDIYEEDVTPKD
                     FGRVGAQAAKQAVMQRLRDAEREILFEEFIDKEEDILTGIIDRVDHRYVYVNLGRIEA
                     VLSEAERSPNEKYIPNERIKVYVNKVEQTTKGPQIYVSRSHPGLLKRLFEQEVPEIYD
                     GTVIVKSVAREAGDRSKISVFSENNDIDAVGACVGAKGARVEAVVEELGGEKIDIVQW
                     NEDPKVFVKNALSPSQVLEVIVDETNQSTVVVVPDYQLSLAIGKRGQNARLAAKLTGW
                     KIDIKSETDAREAVIYPVVEAEKVTEEDVALEDADTTESTEEVNDVSVETNVEKESE"
     gene            23075..>23302
                     /locus_tag="ACGEXS_000721"
     CDS             23075..>23302
                     /locus_tag="ACGEXS_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFF8011759.1"
                     /translation="MKKKKIPMRKCILSNEMHPKKDMIRVVVNKEGEIFADVTGKKQG
                     RGAYVSKDVAMVEKAQQKEILEKYFKASKEQL"
BASE COUNT         8559 a         3248 c         4606 g         6889 t
ORIGIN      
        1 atgatttgtt tagatataaa acgttgacaa aagcaaaatt aagcgtttat catttatctt
       61 tagtaattag attagcgagg gggaaatgac attggcagat aatttagtca ttgttgaatc
      121 gcctgcaaaa gcaaaaacca ttgaaaagta tttaggtaag aaatataaag ttatagcttc
      181 aatgggacac gtcagagact taccaagaag tcaaatgggt gtcgacactg aagataatta
      241 cgaaccaaaa tatataacaa tacgcggaaa aggtcctgtt gtaaaagaat tgaaaaaaca
      301 tgcaaaaaaa gcgaaaaacg tctttctcgc aagtgacccc gaccgtgaag gtgaagcaat
      361 tgcttggcat ttatcaaaaa ttttagagct tgaagattct aaagaaaatc gcgttgtttt
      421 caacgaaata actaaagacg ctgttaaaga aagttttaaa aatcctagag aaattgaaat
      481 gaacttagtc gatgcacaac aagcgcgtcg aatattagat agattagttg gctataacat
      541 ctcgccagtt ctttggaaaa aagtaaaaaa agggttgtca gcgggtcgag ttcaatctgt
      601 tgcacttcgt ttagtcattg accgtgaaaa tgaaattcga aactttaaac cagaagaata
      661 ttggactatt gaaggagaat ttagatacaa aaaatcaaaa ttcaatgcta aattccttca
      721 ttataaaaat aaacctttta aactaaaaac gaaaaaagat gttgagaaaa ttacagctgc
      781 attagatgga gatcaattcg aaattacaaa cgtgactaaa aaagaaaaaa cgcgtaatcc
      841 agcaaaccca tttacaactt ctacattaca acaagaggcg gcacgtaaat taaactttaa
      901 agcaagaaaa acaatgatgg tcgcacaaca attatatgaa ggtatagatt tgaaaaaaca
      961 aggtacgatt ggtttaataa catatatgag aaccgattct acacgtattt cagatactgc
     1021 caaagttgaa gcaaaacagt atataactga taaatacggt gaatcttaca cttctaaacg
     1081 taaagcatca gggaaacaag gtgaccaaga tgcccatgag gctattagac cttcaagtac
     1141 tatgcgtacg ccagatgata tgaagtcatt tttgacgaaa gaccaatacc gattatacaa
     1201 attaatttgg gaacgatttg ttgctagtca aatggctcca gcaatacttg atacagtctc
     1261 attagacata acacaaggtg acattaaatt tagagcgaat ggtcaaacaa tcaagtttaa
     1321 aggatttatg acactttatg tagaaactaa agatgatagt gatagcgaaa aggaaaataa
     1381 actgcctaaa ttagagcaag gtgataaagt cacagcaact caaattgaac cagctcaaca
     1441 ctatacacaa ccacctccaa gatatactga ggcgagatta gtaaaaacac tagaagaatt
     1501 gaaaattggg cgaccatcaa cttatgcacc gacaatagat acgattcaaa agcgtaacta
     1561 tgtcaaatta gaaagtaagc gttttgttcc tactgagttg ggagaaatag ttcatgaaca
     1621 agtgaaagaa tacttcccag agattattga tgtggaattc acagtgaata tggaaacgtt
     1681 acttgataag attgcagaag gcgacattac atggaggaaa gtaatcgacg gtttctttag
     1741 tagctttaaa caagatgttg aacgtgctga agaagagatg gaaaagattg aaatcaaaga
     1801 tgagccagcc ggtgaagact gtgaaatttg tggttctcct atggttataa aaatgggacg
     1861 ctatggtaag ttcatggctt gctcaaactt cccggattgt cgtaatacaa aagcgatagt
     1921 taagtctatt ggtgttaaat gtccaaaatg taatgatggt gacgtcgtag aaagaaaatc
     1981 taaaaagaat cgtgtctttt atggatgttc gaaatatcct gaatgcgact ttatctcttg
     2041 ggataagccg attggaagag attgtccaaa atgtaaccaa tatcttgttg aaaataaaaa
     2101 aggcaagaca acacaagtaa tatgttcaaa ttgcgattat aaagaggcag cgcagaaata
     2161 atatttttat ttcctagata cattttaaga ttgttaaata gaatcattag tgaatcttat
     2221 tttaaagata gtaaaggatt aatctaaata agtgcggata atataaacat aacaacataa
     2281 ttaaaagaca taaatgacaa taaaaggagt atagaaatga ctcaaactgt aaatgtaata
     2341 ggtgctggtc ttgccggttc agaagcggca tatcaattag ctgaaagagg aattaaagtt
     2401 aatctaatag agatgagacc tgttaaacaa acaccagcgc accatactga taaatttgcg
     2461 gaacttgtat gttccaattc attacgcgga aatgctttaa ctaatggtgt gggtgtttta
     2521 aaagaagaaa tgagaagatt gaattctata attattgaag cggctgataa ggcacgagtt
     2581 ccagctggtg gtgcattagc agttgataga cacgattttt caggttatat tactgaaaca
     2641 cttaaaaatc atgaaaatat cacagttatt aatgaagaaa ttaatgccat tccagatgga
     2701 tacacaatta tcgcaacagg accacttact acagaaaccc ttgcgcaaga aatagtggac
     2761 attactggta aagatcaact ttatttctat gatgcggctg ctccaattat tgaaaaagaa
     2821 tctattgata tggataaagt ttacttaaag tcccgttatg ataaaggtga agctgcatat
     2881 ttaaactgtc ctatgactga ggatgaattt aatcgctttt atgatgcagt attagaagct
     2941 gaagttgcgc ctgtaaattc atttgaaaaa gaaaaatatt tcgagggttg tatgcctttt
     3001 gaagtaatgg cagaacgcgg acgcaagaca ttactatttg gaccaatgaa accagtagga
     3061 ttagaagatc caaagactgg gaaacgtcct tatgcggtgg ttcaattaag acaagatgac
     3121 gctgctggta cactctacaa tattgttggc ttccaaacgc atttaaaatg gggagctcaa
     3181 aaagaagtca ttaaattaat tccaggctta gaaaatgttg atattgttag atatggtgtg
     3241 atgcatagaa ataccttcat taattcaccg gacgtattaa acgagaaata tgaattgatt
     3301 tcacaaccaa acatacagtt tgcgggacaa atgactggtg ttgaaggtta tgtagaaagc
     3361 gcagctagcg gcttagttgc aggtatcaat cttgcgcata aaatattagg caagggtgag
     3421 gtagtatttc cgagagaaac aatgattgga agtatggctt actatatttc tcatgctaaa
     3481 aacaataaga atttccaacc tatgaatgct aacttcgggt tattaccatc tttagaaact
     3541 agaattaaag ataaaaaaga acgctatgaa gcacaagcta atagagcttt ggattactta
     3601 gaaaatttca aaaaaacttt ataaaatagt tagaaagact agatatgcta ttcattctta
     3661 agtcatcaac gagtaagtaa tgactttcta aatggaaaat acttatccta gtctttttaa
     3721 ttttggaatt gttacgtatt tctgacaatt tagaattcgc attcaaaaaa tatctaaata
     3781 aataacacgc aataagttga ttgatgtaac atgtaagaga atgttttaaa taaactttat
     3841 ttaaaaggca atgaaataat aaatggcaag gctattaata aagactttta gtaattaatt
     3901 taaaaaagag gtattctaat taacaggttt tccgattagt tacaattatt taattctcaa
     3961 aagatttaga attgattatc aaattactgt aagccctttg ctgtatatgc tacaattctt
     4021 attgatggag ggtaaatgta ttgaatcata ttcaagatgc gtttttaaat acattgaaag
     4081 ttgaacggaa tttttcggaa cacacattga aatcatatca agatgactta attcagttta
     4141 atcaattttt agaacaagaa catttagagt tgaatacttt tgaatacaga gatgctagaa
     4201 attatttgag ctatttatat tcaaatcatt tgaaaagaac atctgtttct cgtaaaatct
     4261 caacgttaag aactttctat gaatattgga tgacgcttga tgagaacatt attaatccat
     4321 ttgttcaatt agtacatccg aaaaaagaaa aatatcttcc gcaattcttt tacgaagaag
     4381 aaatggaagc gttattcaaa actgtagaag aggacacttc aaaaaattta cgggatcgag
     4441 ttattcttga attgttgtat gctacaggca tccgtgtttc ggaattagta aatattaaaa
     4501 aacaagatat agatttttac gcgaatggtg ttaccgtatt aggaaaaggg agcaaagagc
     4561 gctttgtacc gtttggtgct tattgtagac aaagcatcga aaattattta gaacatttca
     4621 aaccaattca gtcatgcaat catgattttc ttattgtaaa tatgaagggt gaagcaatca
     4681 ctgaacgcgg tgtacgatat gttttaaatg atattgttaa acgaacagca ggcgtaagtg
     4741 agattcatcc ccacaagctc agacatacat ttgcaacgca tttattgaat caaggtgcag
     4801 acctaagaac agtacaatcg ttattaggtc atgttaattt gtcaacaact ggtaaatata
     4861 cacacgtatc taaccaacaa ttaagaaaag tgtatctaaa tgcacatcct cgagcgaaaa
     4921 aggagaatga aacatgagta atacaacatt acatgcaaca acaatttatg ctgtaagaca
     4981 taatgggaaa gcagctatgg ctggagatgg gcaagtaacg cttggtcaac aagtcatcat
     5041 gaaacaaacg gcaagaaaag tgcgacgttt atatgaaggt aaagtgttag ctggtttcgc
     5101 aggtagtgta gcagatgcgt ttacgttatt tgaaaaattc gaaacaaaat tacaacagtt
     5161 tagtggtaac ttagaaagag ctgctgttga attggcacaa gaatggcgag gcgataaaca
     5221 attacgtcaa ttagaagcta tgctaattgt aatggataaa gatgctattt tagttgtcag
     5281 tggaactggc gaagttattg ctccagatga tgaccttatc gctattggat caggaggcaa
     5341 ctacgcatta agcgcaggac gtgcattgaa acgccatgca tcgcatttgt ctgctgaaga
     5401 aatggcatat gagagcttga aagtagcggc tgatatttgt gtctttacca acgataatat
     5461 tgttgtcgaa acactataat aatcagagca cgataaataa ttacgagcaa ttaattttag
     5521 ttaaaagacg gaggaatgaa attaatggat acagctggaa taagattaac tccaaaagaa
     5581 atcgtatcta aattaaatga atacatcgtt ggacaaaatg atgctaaacg taaagtggca
     5641 attgccctac gtaatcgata cagaagaagt ttattagatg aggaatcaaa gcaagaaatt
     5701 tcacctaaaa atattttgat gattggacca accggcgttg gtaaaactga aattgcaaga
     5761 agaatggcca aagttgtcgg cgcgccattt ataaaagtag aagctactaa atttactgag
     5821 gtaggttatg taggacgaga tgttgaaagt atggttagag atcttgttga tgtttcagta
     5881 agattagtca aggcgcagaa aaaatcattg gtacaagatg aagcaacagc taaggccaat
     5941 gaaaaacttg ttaagttatt agttccaagt atgaaaaaga aagcgtctca aacgaataat
     6001 cctttagagt cacttttcgg aggtgcaatt ccaaatttcg gacaaaataa cgaagatgaa
     6061 gaagaaccac ctactgagga aattaaaaca aaacgttctg aaattaagag acagctagaa
     6121 gaaggcaaac ttgaaaaaga aaaggtaaga attaaagtcg aacaagatcc tggtgcttta
     6181 ggtatgctag gtacaaatca aaatcagcaa atgcaagaga tgatgaatca attaatgcct
     6241 aaaaagaaag ttgagcgaga agttgctgtt gagacggcaa ggaaaatctt agctgatagt
     6301 tatgcggatg aactaattga tcaagaaagc gctaaccaag aagcgcttga attagcagaa
     6361 caaatgggta tcatctttat agatgaaatc gacaaagttg cgacgaataa tcataatagt
     6421 ggtcaagatg tctcaagaca aggtgttcaa agagatattt tacctatact tgaaggtagc
     6481 gttattcaaa ccaaatatgg tactgtgaat actgaacata tgctgtttat aggtgctgga
     6541 gctttccatg tatctaagcc gagtgacttg ataccagaat tgcaaggtcg ttttccgatt
     6601 agagttgaac ttgatagttt atcggtagaa gattttgtaa gaattttgac agaaccaaaa
     6661 ttgtcattaa ttaaacaata tgaagcattg cttcaaacag aagaagttac tgtaaacttt
     6721 accgatgaag caattactcg cttagctgag attgcttatc aagtaaatca agatacagac
     6781 aacattggtg cacgtcgact tcatacaatt ttagaaaaga tgctagaaga tttatcattc
     6841 gaagcaccaa gtatgccgaa tgcagttgta gatattaccc cacaatatgt tgatgataaa
     6901 ttaaaatcaa tttcaacaaa taaagattta agtgcattta ttctataaaa atatacaaaa
     6961 ggagaaaaat tcatgagctt attatctaaa acgagagagt taaacacgtt acttcaaaaa
     7021 cacaaaggta ttgcggttga ttttaaagat gtagcacaaa cgattagtag cgtaactgta
     7081 acaaatgtat ttattgtatc gcgtcgaggt aaaattttag gatcgagtct aaatgaatta
     7141 ttaaaaagtc aaagaattat tcaaatgttg gaagaaagac atattccaag tgaatataca
     7201 gaacgattaa tggaagttaa acaaacagaa tcaaatattg atatcgacaa tgtattaaca
     7261 gtattcccac ctgaaaacag agaattattc atagatagtc gtacaactat cttcccaatt
     7321 ttaggtggag gggaaagatt aggtacatta gtacttggtc gagtacatga tgattttaat
     7381 gaaaatgatt tggtactagg tgaatatgct gctacagtta ttggtatgga aatcttacgt
     7441 gagaagcata gtgaagtaga aaaagaagcg cgcgataaag ctgctattac aatggcaatt
     7501 aattcattat cttattctga aaaagaagcg attgaacata tctttgaaga acttggcggt
     7561 acggaaggcc tattaatcgc atcaaaagtt gcagatagag ttggtattac tagatctgta
     7621 attgtaaatg cactacgtaa attagaaagt gctggtgtaa ttgaatcacg ttctttagga
     7681 atgaaaggta ctttcattaa agttaaaaaa gaaaaattct tagatgaatt agaaaaaagt
     7741 aaataagtcg atgagtctgg gacataattc aatgtcctag tcgtgacgat gttatcttgg
     7801 cggtagtcaa tgaaatgact ttgatgatta tgttgaagtc atttctttat ttttgtctaa
     7861 ttttcaatca aattgatatt tcacattaac ttgatagaat tacctttaaa tttatggaaa
     7921 aatgcttgaa aaacatttgc tttagacacg gcattatgtt atatttattt tcggctataa
     7981 agccaataca cacatatcaa acgtgattgt gtaaagggtg caatatttat atattgtctt
     8041 tacaatagtt tgctatggag gtaattaacc aataggagga atttataatg gcagtaattt
     8101 caatgaaaca attactagaa gcgggtgttc acttcggtca ccaaacacgt cgttggaacc
     8161 caaaaatgaa aaaatatatc ttcactgaga gaaatggtat ttatatcatc gacttacaaa
     8221 aaacagtgaa aaaagtagac gaggcataca acttcttgaa acaagtttca gaagatggtg
     8281 gacaagtctt attcgtagga actaaaaaac aagcacaaga atcagttaaa tctgaagcag
     8341 aacgtgctgg tcaattctac attaaccaaa gatggttagg tggattatta actaactata
     8401 aaacgatctc aaaacgaatc aaacgtattt ctgaaattga aaaaatggaa gaagatggtt
     8461 tattcgaagt attacctaaa aaagaagtag tagaacttaa aaaagaatac gaccgtttaa
     8521 tcaaattctt aggcggaatt cgtgatatga aatcaatgcc tcaagcatta ttcgtagttg
     8581 acccacgtaa agagcgtaat gcaattgctg aagctcgtaa attaaatatt cctatcgtag
     8641 gtatcgttga cactaactgt gatcctgacg aaattgacta cgttatccca gcaaacgacg
     8701 atgctatccg tgcggttaaa ttattaactg ctaaaatggc agatgcaatc ttagaaggtc
     8761 aacaaggcgt ttctaatgaa gaagtagctg cagaacaaaa catcgattta gatgaaaaag
     8821 aaaaatcaga agaaacagaa gcaactgaag aataatcaac tgttgaatct gacttagata
     8881 tagtttaaat gggtgataag atattaatgc ttatcacctt ttttaaaaag aaaatcgagg
     8941 caaattacaa atattcaatt agagtattgg caatcttgcc tataataatg ctaaaatcat
     9001 aatatataaa atgataactt attggaggaa taatgaatgg caactatttc agcaaaactt
     9061 gttaaagaat tacgtgaaaa aactggcgcg ggtatgatgg attgtaaaaa agcgctaact
     9121 gaaactgatg gtgacatcga taaagcgatt gactacctac gtgaaaaagg tattgctaaa
     9181 gcagctaaaa aagcagaccg tattgcggct gaaggtttag tacatgtaga aactaaaggt
     9241 aacgacgcag ttatcgttga aatcaactct gaaacagact ttgttgctcg taacgaaggt
     9301 ttccaagagt tagttaaaga aatcgctaat caagtattag atacaaaagc tgaaactgtt
     9361 gaagctttaa tggaaacaac tttaccaaat ggtaaatcag ttgatgaaag aattaaagaa
     9421 gcaatttcaa caatcggtga aaaattaagt gttcgtcgtt ttgctatcag aactaaaact
     9481 gataacgatg ctttcggcgc ttacttacac atgggtggac gcattggtgt attaacagtt
     9541 gttgaaggtt caactgacga agaagcagca agagacgttg ctatgcatat cgctgcaatc
     9601 aaccctaaat atgtttcttc tgaacaagtt agcgaagaag aaatcaacca cgaaagagaa
     9661 gttttaaaac aacaagcatt aaatgaaggt aaaccagaaa acatcgttga aaaaatggtg
     9721 gaaggacgtt tacgtaaata cttacaagaa atttgtgctg tagatcaaga cttcgttaaa
     9781 aaccctgatg taacagttga agctttctta aaaacaaaag gtggaaaact tgttgacttc
     9841 gtacgctatg aagtaggcga aggtatggaa aaacgcgaag aaaactttgc ggatgaagtt
     9901 aaaggacaaa tgaaataatc tgtcataaag taaacaagga agaagacacc tttaatgttg
     9961 ctttattaaa atgtaaatca ttctaataaa acgacaactg tgtcttcttt acttgtatat
    10021 gttacatata ttcacgatag agaggataag aaaatggctc aaatttctaa atataaacgt
    10081 gtagttttga aactaagtgg tgaagcgtta gctggagaaa aaggatttgg cataaatcca
    10141 gtaattatta aaagtgttgc tgagcaagtg gctgaagttg ctaaaatgga ctgtgaaatc
    10201 gcagtaatcg ttggtggcgg aaacatttgg agaggtaaaa caggtagtga cttaggtatg
    10261 gaccgtggaa ctgctgatta catgggtatg cttgcaactg taatgaatgc cttagcatta
    10321 caagatagtt tagaacaatt ggattgtgat acacgagtat taacatctat tgaaatgaag
    10381 caagtggctg aaccttatat tcgtcgtcgt gcaattagac acttagaaaa gaaacgcgta
    10441 gttatttttg ctgcaggtat tggaaaccca tacttctcta cagatactac agcggcatta
    10501 cgtgctgcag aagttgaagc agatgttatt ttaatgggca aaaataatgt agatggtgta
    10561 tattctgcag atcctaaagt aaacaaagat gcggtaaaat atgaacattt aacgcatatt
    10621 caaatgcttc aagaaggttt acaagtaatg gattcaacag catcctcatt ctgtatggat
    10681 aataacattc cgttaactgt tttctctatt atggaagaag gaaatattaa acgtgctgtt
    10741 atgggtgaaa agataggtac gttaattaca aaataaattt agaggtgtaa aataatgagt
    10801 gacattatta atgaaactaa atcaagaatg caaaaatcaa tcgaaagctt atcacgtgaa
    10861 ttagctaaca tcagtgcagg aagagctaat tcaaatttat taaacggcgt aacagttgat
    10921 tactatggtg caccaacacc tgtacaacaa ttagcaagca tcaatgttcc agaagcacgt
    10981 ttacttgtta tttctccata cgacaaaact tctgtagctg acatcgaaaa agcgataata
    11041 gcagctaact taggtgttaa cccaacaagt gatggtgaag tgatacgtat tgctgtacct
    11101 gccttaacag aagaacgtag aaaagagcgc gttaaagatg ttaagaaaat tggtgaagaa
    11161 gctaaagtat ctgttcgaaa tattcgtcgt gatatgaatg atcagttgaa aaaagatgaa
    11221 aaaaatggcg acattactga agatgagttg agaagtggta ctgaagatgt tcagaaagca
    11281 acagacaatt caataaaaga aattgatcaa atgattgctg ataaagaaaa agatattatg
    11341 tcagtataaa actaatatac aatgacatat taaaatgcca gtattaaacg ataatgtaac
    11401 atttaaaatg ggcatgttta attaaatcaa agatgcatgt gataatttaa attcacaatg
    11461 agcataaaaa tggtgtttaa acaagttaat taaacatata ctttataaat aataggcatt
    11521 aggtatattg ctataataaa gttatgtaat ttttaacctc agtatgtatg tcacatttct
    11581 ggtgtaaact gtaccgagtc agactttggt acagtttttt tatttgctta ttcaatgcat
    11641 taaatgagta tgataaaatg ataatgattg tttagtaact tatactatat gacagagatg
    11701 atcaggctcg gaggaaagac catgtttaaa aagctaataa ataaaaagaa cactataaat
    11761 aattataatg aagaattaga ctcgtctaat atacctgaac atatcgctat tattatggat
    11821 ggtaatgggc gatgggctaa gaagcgaaaa atgcctagaa ttaaaggtca ttacgaaggt
    11881 atgcaaacaa taaaaaaaat tactagggta gctagtgata ttggtgttaa gtacttaact
    11941 ttatacgcct tttccactga aaattggtca agacctgaaa gtgaagtaaa ttatattatg
    12001 aatttgcctg tcaatttctt aaagacattc ttaccggaac taattgaaaa aaatgtcaaa
    12061 gttgaaacaa ttggatttac tgataagttg ccaaaatcaa cgatagaagc aattaataat
    12121 gctaaagaaa agacagctaa taataccggc ttaaaattaa tatttgcaat taattatggt
    12181 ggcagagcag aacttgttca tagtattaaa aatatgtttg acgagcttca tcaacaaggt
    12241 ttaaatagtg atatcataga tgaaacatat ataaacaatc atttaatgac aaaagactat
    12301 cctgatccag agttgttaat tcgtacttca ggagaacaaa gaataagtaa tttcttgatt
    12361 tggcaagttt cgtatagtga atttatcttt aatcaaaaat tatggcctga ctttgacgaa
    12421 gatgaattaa ttaaatgtat aaaaatttat cagtcacgtc aaagacgctt tggcggattg
    12481 agtgaggagt agtatagtat gaaagttaga acgctgacag ctattattgc cttaatcgta
    12541 ttcttgccta tcttgttaaa aggcggcctt gtgttaatga tatttgctaa tatattagca
    12601 ttgattgcat taaaagaatt gttgaatatg aatatgatta aatttgtttc agttcctggt
    12661 ttaattagtg cagttggtct tatcatcatt atgttgccac aacatgcagg gccatgggta
    12721 caagtaattc aattaaaaag tttaattgca atgagcttta ttgtattaag ttatactgtc
    12781 ttatctaaaa acagatttag ttttatggat gctgcatttt gcttaatgtc tgtggcttat
    12841 gtaggcattg gttttatgtt cttttatgaa acgagatcag aaggattaca ttacatatta
    12901 tatgcctttt taattgtttg gcttacagat acaggggctt acttgtttgg taaaatgatg
    12961 ggtaaacata agctttggcc agtaataagt ccgaataaaa caatcgaagg attcataggt
    13021 ggcttgttct gtagtttgat agtaccactt gcaatgttat attttgtaga tttcaatatg
    13081 aatgtatgga tattacttgg agtgacattg attttaagtt tatttggtca attaggtgat
    13141 ttagtggaat caggatttaa gcgtcatttc ggcgttaaag actcaggtcg aatactacct
    13201 ggacacggtg gtattttaga ccgatttgac agctttatgt ttgtgttacc attattaaat
    13261 attttattaa tacaatctta atgctgagaa caaatcaata aacgtaaaga ggagttgctg
    13321 agataattta atgaatctca gaactccttt tgaaaattat acgcaatatt aactttgaaa
    13381 attatacgca atattaactt tgaaaattag acgttatatt ttgtgatttg tcagtatcat
    13441 attataatga cttatgttac gtatacagca atcattttta aaataaaaga aatttataaa
    13501 caatcgaggt gtagcgagtg agctatttag ttacaataat tgcatttatt attgtttttg
    13561 gtgtactagt aactgttcat gaatatggcc atatgttttt tgcgaaaaga gcaggcatta
    13621 tgtgtccaga atttgcgatc ggtatggggc caaaaatttt tagttttaga aaaaatgaaa
    13681 cactttacac tattaggtta ttgcctgttg gtggatatgt tcgtatggca ggagatggct
    13741 tagaagagcc accagtcgag cccggtatga acgttaaaat taaacttaat gaagaaaatg
    13801 aaataacaca tatcatatta gatgatcatc ataagtttca acaaattgaa gcgatcgaag
    13861 ttaaaaaatg tgattttaag gatgacttat tcatagaagg tatcactgct tatgataatg
    13921 aaagacatca ttttaaaatt gctagaaagt ctttctttgt tgaaaatggt agcttagttc
    13981 aaattgctcc gagagacaga caatttgcac ataaaaagcc atggccgaaa tttttaacat
    14041 tatttgcggg accgttattt aactttatat tagctttagt cctatttatt ggtcttgcat
    14101 attatcaagg cacgcctacg tctactgtag aacaagtcgc agataagtat ccagctcaac
    14161 aagcaggatt acaaaaaggt gataagatcg tccaaattgg caaatataaa atatctgaat
    14221 ttgatgatgt tgataaggcg ttagataaag ttaaagataa taagacgact gttaaatttg
    14281 aacgtgatgg taaaacaaag tcagttgaat taacacctaa aaagactgaa aaaaaactga
    14341 ctaaagtaag ttcagagacg aagtatgttc tcggattcca accagcgagt gaacatacac
    14401 tttttaaacc aattgtattc ggatttaaaa gctttttaat cggtagtact tatattttta
    14461 cagctgtagt aggtatgttg gctagtatat ttacgggcgg attctcattt gatatgttaa
    14521 atggtccggt tggtatttat cataacgtcg actcagttgt taaagcgggt atcattagct
    14581 taattggtta cactgcgtta ttaagtgtaa acttaggtat tatgaattta attcctattc
    14641 ctgcactaga cggtggtcgt attttatttg ttatatatga agcgattttc agaaaaccag
    14701 ttaataaaaa agcggaaaca acgattattg ctattggtgc cattttcatg gtcgttataa
    14761 tgatattagt aacgtggaat gatattcgac gatatttctt ataatttagg aggataaata
    14821 attatgaagc aatccaaagt ttttatacca acgatgcgtg acgtgccatc agaagcagaa
    14881 gcacaaagtc atcgtttatt attgaaatcg ggtttgataa aacaaagtac aagtgggatt
    14941 tatagttatt taccgctagc aacacgtgtg ttaaataata ttactgcaat tgtgcgacaa
    15001 gaaatggaac gtatcgattc tgttgaaatt ttaatgccag cgttacaaca agctgaatta
    15061 tgggaagaat caggacgttg gggtgcatat ggcccagaat taatgcgttt acaagataga
    15121 catggaagac aatttgcatt aggtccaaca catgaagaat tagttacatc aatagtaaga
    15181 aatgaattga aatcatacaa acaattaccg atgacattat tccaaattca atctaaattc
    15241 cgtgatgaaa agagaccacg ttttggttta cttcgtgggc gtgaatttat tatgaaagat
    15301 gcatattcat tccatgctga cgaggcatca ttagatcaaa cgtatcaaga tatgtatcaa
    15361 gcgtatagcc gtatttttga gagagttggc attaacgcaa gaccagtagt tgcagattca
    15421 ggtgctatag gcggtagcca tacacatgaa tttatggcat taagtgctat cggtgaggat
    15481 acaatcgttt acagtaaaga aagtgattat gctgctaaca tcgaaaaagc agaagtcgtt
    15541 tacgaaccaa atcataagca tactactgtg caacctttag aaaaaattga aacaccaaat
    15601 gttaagactg cgcaagaatt ggcagacttc ttaggtagac cagtagatga aatcgttaaa
    15661 acgatgattt tcaaagttga tggcgaatat attatggttt tagtgcgtgg ccatcatgaa
    15721 attaatgaca ttaaattaaa atcttatttc ggcacagata atattgaatt agcaacacaa
    15781 gacgaaattg ttaatttagt tggtgcaaat cctggttcac taggtcctgt aattgataaa
    15841 gaaatcaaaa tttatgcaga taattttgtg caagatttaa ataatttagt tgtcggtgct
    15901 aacgaagatg gttatcactt aattaatgta aatgtaggta gagacttcaa cgttgatgaa
    15961 tatggcgatt tccgttttat tttagaaggc gaaaagttaa gtgatggttc aggcgttgca
    16021 cattttgctg aaggtattga agttggtcaa gtattcaaat tgggtactaa gtattcagaa
    16081 tcaatgaatg ctacattctt agataaccaa ggaaaagctc aatctttaat tatgggttgt
    16141 tacggaattg gaatttctag aacgctaagt gcgattgttg aacaaaatca cgatgataat
    16201 ggaattgttt ggcctaaatc agttactccg tttgatttac atttaatttc tattaatcct
    16261 aagaaagatg atcaacgaga actagcagat gcactatatg ctgaatttaa tactaaattt
    16321 gatgtgttgt acgatgatcg tcaggaacgt gcaggtgtta aatttaatga tgccgattta
    16381 attggtttac cactgcgaat tgttgttggt aaacgtgcat cggaaggtat tgtagaagtt
    16441 aaagaacgtt taacaggtga tagcgaagaa gttcacattg atgacttaat gactgtcatt
    16501 acaaataaat atgataactt aaaataatta agatcgaatg aattataaga gtaggaaaaa
    16561 gctgaaagaa atctgatgct tatgtcctgc tcttattatt tttgatataa tgattattcg
    16621 atgaaaaatg actgaagaca tagtataatt aaagataaat ttgttttaac aatataatga
    16681 ttagccaaat ataaagcatt taattttcta tcattactat gctcacataa tctaaatatt
    16741 gttcgaacac gtaaaagtaa tttctattta aggtggtaat tgtcttggca atgacagagc
    16801 aacaaaaatt taaagtgctt gctgatcaaa ttaaaatttc aaatcaatta gatgctgaaa
    16861 ttttaaattc aggtgaactg acacgtatag atgtttctaa caaaaacaga acatgggaat
    16921 ttcatattac attaccacaa ttcttagctc atgaagatta tttattattt ataaatgcaa
    16981 tagagcaaga gtttaaagat atcgccaacg ttacatgtcg ttttacggta acaaatggca
    17041 cgaatcaaga tgaacatgca attaaatact ttgggcactg tattgaccaa acagctttat
    17101 ctccaaaagt taaaggtcaa ttgaaacaga aaaagcttat tatgtctgga aaagtattaa
    17161 aagtaatggt atcaaatgac attgaacgta atcattttga taaggcatgt aatggaagtc
    17221 ttatcaaagc gtttagaaat tgtggttttg atatcgataa aatcatattc gaaacaaatg
    17281 ataatgatca agaacaaaac ttagcttctt tagaagcaca tattcaagaa gaagacgaac
    17341 aaagtgcacg attggcaaca gagaaacttg aaaaaatgaa agctgaaaaa gcgaaacaac
    17401 aagataacaa cgaaagtgct gtcgataagt gtcaaattgg taagccgatt caaattgaaa
    17461 atattaaacc aattgaatct attattgagg aagagtttaa agttgcaata gagggtgtca
    17521 tttttgatat aaacttaaaa gaacttaaaa gtggtcgcca tatcgtagaa attaaagtga
    17581 ctgactatac ggactcttta gttttaaaaa tgtttactcg taaaaacaaa gatgatttag
    17641 aacattttaa agcgctaagt gttggtaaat gggttagggc tcaaggtcgt attgaagaag
    17701 atacatttat tagagattta gttatgatga tgtctgatat tgaagagatt aaaaaagcga
    17761 caaaaaaaga taaggctgaa gaaaagcgtg tagaattcca cttgcatact gcaatgagcc
    17821 aaatggatgg tatacccaat attggtgcgt atgttaaaca ggcagcagac tggggacatc
    17881 cagccattgc ggttacagac cataatgttg tgcaagcatt tccagatgct cacgcagcag
    17941 cggaaaaaca tggcattaaa atgatatacg gtatggaagg tatgttagtt gatgatggtg
    18001 ttccgattgc atacaaacca caagatgtcg tattaaaaga tgctacttat gttgtgttcg
    18061 acgttgagac aactggttta tcaaatcagt atgataaaat catcgagctt gcagctgtga
    18121 aagttcataa cggtgaaatc atcgataagt ttgaaaggtt tagtaatccg catgaacgat
    18181 tatcggaaac gattatcaat ttgacgcata ttactgatga tatgttagta gatgcccctg
    18241 agattgaaga agtacttaca gagtttaaag aatgggttgg cgatgcgata ttcgtagcgc
    18301 ataatgcttc gtttgatatg ggcttcatcg atacgggata tgaacgtctt gggtttggac
    18361 catcaacgaa tggtgttatc gatactttag aattatctcg tacgattaat actgaatatg
    18421 gtaaacatgg tttgaatttc ttggctaaaa aatatggcgt agaattaacg caacatcacc
    18481 gtgccattta tgatacagaa gcaacagctt acattttcat aaaaatggtt caacaaatga
    18541 aagaattagg cgtattaaat cataacgaaa tcaacaaaaa actcagtaat gaagatgcat
    18601 ataaacgtgc aagacctagt catgtcacat taattgtaca aaaccaacaa ggtcttaaaa
    18661 atctatttaa aattgtaagt gcatcattgg tgaagtattt ctaccgtaca cctcgaattc
    18721 cacgttcatt gttagatgaa tatcgtgagg gattattggt aggtacagcg tgtgatgaag
    18781 gtgaattatt tacggcagtt atgcagaagg accagagtca agttgaaaaa attgccaaat
    18841 attatgattt tattgaaatt caaccaccgg cactttatca agatttaatt gatagagagc
    18901 ttattagaga tactgaaaca ttacatgaaa tttatcaacg tttaatacat gcaggtgaca
    18961 cagcgggtat acctgttatt gcgacaggaa atgcacacta tttgtttgaa catgatggta
    19021 tcgcacgtaa aattttaata gcatcacaac ccggcaatcc acttaatcgc tcaactttac
    19081 cggaagcaca ttttagaact acagatgaaa tgttaaacga gtttcatttt ttaggtgaag
    19141 aaaaagcgca tgaaattgtt gtgaaaaata caaacgaatt agcagatcga attgaacgtg
    19201 ttgttcctat taaagatgaa ttatacacac cgcgtatgga aggtgctaac gaagaaatta
    19261 gagaactaag ttatgcaaat gcgcgtaaac tgtatggtga agacctgcct caaatcgtaa
    19321 ttgatcgatt agaaaaagaa ttaaaaagta ttatcggtaa tggatttgcg gtaatttact
    19381 taatttcgca acgtttagtt aaaaaatcat tagatgatgg atacttagtt ggttcccgtg
    19441 gttcagtagg ttctagtttt gtagcgacaa tgactgagat tactgaagta aacccgttac
    19501 cgccacacta tatttgtccg aactgtaaaa cgagtgaatt tttcaatgat ggttcagtag
    19561 gatcaggatt tgatttacct gataagacgt gtgaaacttg tggagcgcca cttattaaag
    19621 aaggacaaga tattccgttt gaaacatttt taggatttaa gggagataaa gttcctgata
    19681 tcgacttaaa ctttagtggt gaatatcaac cgaatgccca taactacaca aaagtattat
    19741 ttggtgagga taaagtattc cgtgcaggta caattggtac tgttgctgaa aagactgctt
    19801 ttggttatgt taaaggttat ttgaatgatc aaggtatcca caaaagaggt gctgaaatag
    19861 atcgactcgt taaaggatgt acaggtgtta aacgtacaac tggacagcat ccagggggta
    19921 ttattgtagt acctgattac atggatattt atgattttac gccgatacaa tatcctgccg
    19981 atgatcaaaa ttcagcatgg atgacgacac attttgattt ccattctatt catgataatg
    20041 tattaaaact tgatatactt ggacacgatg atccaacaat gattcgtatg cttcaagatt
    20101 tatcaggaat tgatccaaaa acaatacctg tagatgataa agaagttatg cagatattta
    20161 gtacacctga aagtttgggt gttactgaag atgaaatttt atgtaaaaca ggtacatttg
    20221 gggtaccaga attcggtaca ggattcgtgc gtcaaatgtt agaagataca aagccaacaa
    20281 cattttctga attagttcaa atctcaggat tatctcatgg tacagatgtg tggttaggca
    20341 atgctcaaga attaattaaa accggtatat gtgatttatc aagtgtaatt ggttgtcgtg
    20401 atgatatcat ggtttattta atgtatgctg gtttagaacc atcaatggct tttaaaataa
    20461 tggagtcagt acgtaaaggt aaaggtttaa ctgaagaaat gattgaaacg atgaaagaaa
    20521 atgaagtgcc agattggtat ttagattcat gtcttaaaat taagtacatg ttccctaaag
    20581 cccatgcagc agcatacgtt ttaatggcag tacgtatcgc atatttcaaa gtacatcatc
    20641 cactttatta ctatgcatct tactttacaa ttcgtgcgtc agactttgat ttaatcacga
    20701 tgattaaaga taaaacaagc attcgaaata ctgtaaaaga catgtattct cgctatatgg
    20761 atctaggtaa aaaagaaaaa gacgtattaa cagtcttgga aattatgaat gaaatggcgc
    20821 atcgaggtta tcgaatgcaa ccgattagtt tagaaaagag tcaggcgttc gaatttatca
    20881 ttgaaggcga tacacttatt ccgccgttca tatcagtgcc tgggcttggc gaaaacgttg
    20941 cgaaacgaat tgttgaagct cgtgacgatg gcccattttt atcaaaagaa gatttaaaca
    21001 aaaaagctgg attatctcag aaaattattg agtatttaga tgagttaggc tcattaccga
    21061 atttaccaga taaagctcaa ctttcgatat ttgatatgta aaatgaaata atcaaggtat
    21121 ttatttaatg cgtatggcgt agtcaaagaa atacaaaatt gttgctggac acaaaattat
    21181 gcccgtattt cttttcaatg tcttacgagt ctattcaaat gtaatggtga aataaaggaa
    21241 caaactttta caagaatctc tgattaatag tgaagtcatt tgtttcaagc ataaacttat
    21301 gctataatta agttgcttaa aaattagtga actcaggcag aagagtggga gattcccgct
    21361 cttttctatt tgccaaaaag ggaggcctgt atgagtaaaa ttacagaaca agtagaagtg
    21421 attgttaaac caattatgga agacttgaat tttgaacttg tagacgttga atatgtcaaa
    21481 gagggtagag atcattttct tagaatctct attgataaag aaggtggcgt agatttaaat
    21541 gattgtacgc tagcttctga aaaaataagt gaagctatgg atgcaaatga tcctattcct
    21601 gaaatgtatt atttagacgt agcgtcacct ggtgcagaac gtccaattaa aaaagaacaa
    21661 gatttccaaa atgcaataac gaaacctgta tttgtttctt tatatgtacc aattgaaggt
    21721 gaaaaggaat ggttaggcat tttacaagaa gtcaataatg aaacaattgt agtacaagtt
    21781 aaaatcaaag caagaacgaa agatatagag ataccgagag acaaaatagc aaaagcacgt
    21841 cacgcagtta tgatttaacg tgatgaggag gaaaaaacgt gtcaagtaat gaattattat
    21901 tagctactga gtatttagaa aaagaaaaga agattcctag agcagtatta attgatgcta
    21961 ttgaagcagc tttaattact gcatacaaaa agaattatga tagtgcaaga aatgtccgtg
    22021 tggaattaaa tatggatcaa ggtactttca aagttatcgc tcgtaaagat gttgttgaag
    22081 aagtatttga cgacagagat gaagtggatt taagtacagc gcttgttaaa aaccctgcat
    22141 atgaaattgg tgatatatac gaagaagatg taacacctaa agattttggt cgtgtaggtg
    22201 ctcaagcagc gaaacaagca gtaatgcaac gtcttcgtga tgctgaacgt gaaattttat
    22261 ttgaagaatt tatagacaaa gaagaagaca tacttactgg aattattgac cgtgttgacc
    22321 atcgttatgt atatgtgaat ttaggtcgta tcgaagctgt tttatctgaa gcagaaagaa
    22381 gtcctaacga aaaatatatt cctaacgaac gtatcaaagt atatgttaac aaagtggaac
    22441 aaacgacaaa aggtcctcaa atctatgttt ctcgtagcca tccaggttta ttaaaacgtt
    22501 tatttgaaca agaagttcca gaaatttacg atggtactgt aattgttaaa tcagtagcac
    22561 gtgaagctgg cgatcgctct aaaattagtg tcttctctga aaacaatgat atagatgctg
    22621 ttggtgcatg tgttggtgct aaaggcgcac gtgttgaagc tgttgttgaa gagctaggtg
    22681 gtgaaaaaat cgacatcgtt caatggaatg aagatccaaa agtatttgta aaaaatgctt
    22741 taagcccttc tcaagtttta gaagttattg ttgatgaaac aaatcaatct acagtagttg
    22801 ttgttcctga ttatcaattg tcattagcga ttggtaaaag aggacaaaac gcacgtctag
    22861 ctgctaaatt aaccggctgg aaaattgata ttaaatcaga aacagatgcg cgtgaagcgg
    22921 ttatctatcc agtagttgaa gctgaaaaag taactgaaga agatgttgct ttagaagatg
    22981 ctgacacaac agaatcaacc gaagaggtaa atgatgtttc agttgaaaca aatgtagaga
    23041 aagaatctga ataataggtt ggagtgaagt atctatgaaa aagaaaaaaa ttccgatgcg
    23101 aaaatgtatt ctttcaaatg aaatgcatcc caaaaaagat atgattcgtg ttgttgttaa
    23161 taaagaaggc gaaatctttg cggatgttac tggaaagaaa caaggccgtg gcgcatatgt
    23221 ttctaaagat gttgctatgg ttgaaaaagc acaacaaaaa gaaattttag aaaaatattt
    23281 taaagcatct aaagagcaat tg
//
LOCUS       DAXWFK010000023        22595 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.023, whole genome shotgun sequence.
ACCESSION   DAXWFK010000023 DAXWFK010000000
VERSION     DAXWFK010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 22595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22595
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.023"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            475..1386
                     /gene="rapZ"
                     /locus_tag="ACGEXS_000722"
     CDS             475..1386
                     /gene="rapZ"
                     /locus_tag="ACGEXS_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFF8011760.1"
                     /translation="MDNNEKEKSKSELLVVTGLSGAGKSLVIQCLEDMGYFCVDNLPP
                     VLLPKFVELMEQGNPSLRKVAIAIDLRGKELFNSLVAVVDKVKSESDVIIDVMFLEAS
                     TEKLISRYKETRRAHPLMEQGKRSLINAINDEREHLSQIRSIANFVIDTTKLSPKELK
                     ERIRRYYEDEEFETFTINVTSFGFKHGIQMDADLVFDVRFLPNPYYVVDLRPLTGLDK
                     DVYNYVMKWKETEIFFEKLTDLLDFMIPGYKKEGKSQLVIAIGCTGGQHRSVALAERL
                     GNYLNEVFEYNVYVHHRDAHIESGEKK"
     gene            1383..2378
                     /locus_tag="ACGEXS_000723"
     CDS             1383..2378
                     /locus_tag="ACGEXS_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HFF8011761.1"
                     /translation="MRQIKVVLIGGGTGLSVMARGLREFPIDITAIVTVADNGGSTGK
                     IRDEMDIPAPGDIRNVIAALSDSESVLSQLFQYRFEENQISGHSLGNLLIAGMTNITN
                     DFGHAIKALSKILNIKGRVIPSTNTSVQLNAVMEDGEIVFGETNIPKKHKKIDRVFLE
                     PNDVQPMEEAIDALREADLIVLGPGSLYTSVISNLCVNGISDALIHSDAPKLYVSNVM
                     TQPGETDGYSVKDHIDAIHRQAGQPFIDYVICSTQTFNAQVLKKYEEKHSKPVEVNKA
                     ELEKESINVKTSSNLVEISENHLVRHNTKVLSTMIYDIALELISTIPFVPSDKRK"
     gene            2488..3432
                     /gene="whiA"
                     /locus_tag="ACGEXS_000724"
     CDS             2488..3432
                     /gene="whiA"
                     /locus_tag="ACGEXS_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFF8011762.1"
                     /translation="MSFASEMKNELTRIDVDEMNAKAELSALIRMNGALSLSNQQFVI
                     NVQTENATTARRIYSLIKRVFNVEVEILVRKKMKLKKNNIYICRTKMKAKEILDELGI
                     LKDGIFTHEIDHSMIQDDEMRRSYLRGAFLAGGSVNNPETSSYHLEIFSQNESHAEGL
                     TKLMNSYELNAKHLERKKGSITYLKEAEKISDFLSLIGGYQALLKFEDVRIVRDMRNS
                     VNRLVNCETANLNKTVSAAMKQVESIKLIDKEIGIENLPDRLREIARIRVEHQEISLK
                     ELGEMVSTGPISKSGVNHRLRKLNDLADKIRNGEQIEL"
     gene            4005..4592
                     /gene="clpP"
                     /locus_tag="ACGEXS_000725"
     CDS             4005..4592
                     /gene="clpP"
                     /locus_tag="ACGEXS_000725"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFF8011763.1"
                     /translation="MNLIPTVIETTNRGERAYDIYSRLLKDRIIMLGSQIDDNVANSI
                     VSQLLFLQAQDSEKDIYLYINSPGGSVTAGFAIYDTIQHIKPDVQTICIGMAASMGSF
                     LLAAGAKGKRFALPNAEVMIHQPLGGAQGQATEIEIAANHILKTREKLNRILSERTGQ
                     SIEKIQKDTDRDNFLTAEEAKEYGLIDEVMVPETK"
     gene            complement(4835..5737)
                     /locus_tag="ACGEXS_000726"
     CDS             complement(4835..5737)
                     /locus_tag="ACGEXS_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFF8011764.1"
                     /translation="MKQYLITGGTGMVGSQLVNEIKKSDSHITILTRHDQISNDKKIS
                     YVNWAKSGWEHKVPQNIDVVINLAGATLNKRWTPEYKQTLMLSRIQSTQALYELFKSR
                     NKAPKALFNASATGYYPPDLFMSYTEVYKTLPFDFLSDIVYQWERFAQQFEQLGTRVV
                     IGRFGIILSNEGGALQTMKLPYEYYIGGKLGSGQQWYSWIHINDLIQAILFLINNESA
                     SGPFNLTAPIPERQNLFGYTLARAMHKPHETWAPSLAMRLILGQMSTVVLDTQKVLPN
                     KIQALGFQFKYSNLKMALEDLIKE"
     gene            6440..7069
                     /locus_tag="ACGEXS_000727"
     CDS             6440..7069
                     /locus_tag="ACGEXS_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4887 domain-containing protein"
                     /protein_id="HFF8011765.1"
                     /translation="MSNQNYDYNKNEDGSKKKMSTTAKVVSIATVLLLLGGLVFAIFA
                     YVDHSNKAKERMLNEQKQEQKEKRQKENAEKERKKKQQEEKEQNELDSQANQYQQLPQ
                     QNQYQYVPPQQQAPTKQRPAKEENDDKASKDESKDKDDKASQDKSDDNQKKTDDNKQP
                     AQPKPQPQQPTPKPNNNQQNNQSNQQAKPQAPQQNSQSTTNKQNNANDK"
     gene            7712..8725
                     /locus_tag="ACGEXS_000728"
     CDS             7712..8725
                     /locus_tag="ACGEXS_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFF8011766.1"
                     /translation="MKDLLQAQQKLIPDLIDKMYKRFSILTTISKNQPVGRRSLSEHM
                     DMTERVLRSETDMLKKQDLIKVKPTGMEITAEGEQLISQLKGYFDIYADDNRLSEGIK
                     NKFQIKEVHVVPGDADNSQSVKTELGRQAGQLLEGILQEDAIVAVTGGSTMACVSEAI
                     HLLPYNVFFVPARGGLGENVVFQANTIAASMAQQAGGYYTTMYVPDNVSETTYNTLLL
                     EPSVINTLDKIKQANVILHGIGDALKMAHRRQSPEKVIEQLQHHQAVGEAFGYYFDTQ
                     GQIVHKVKTIGLQLEDLESKDFIFAVAGGKSKGEAIKAYLTIAPKNTVLITDEAAAKI
                     ILE"
     gene            8778..9788
                     /gene="gap"
                     /locus_tag="ACGEXS_000729"
     CDS             8778..9788
                     /gene="gap"
                     /locus_tag="ACGEXS_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFF8011767.1"
                     /translation="MAVKVAINGFGRIGRLAFRRIQEVEGLEVVAVNDLTDDDMLAHL
                     LKYDTMQGRFTGEVEVVDGGFRVNGKEVKSFSEPDASKLPWKDLNIDVVLECTGFYTD
                     KDKAQAHIEAGAKKVLISAPATGDLKTIVFNTNHQELDGSETVVSGASCTTNSLAPVA
                     KVLNDDFGLVEGLMTTIHAYTGDQNTQDAPHRKGDKRRARAAAENIIPNSTGAAKAIG
                     KVIPEIDGKLDGGAQRVPVATGSLTELTVVLEKQDVTVEQVNEAMKNASNESFGYTED
                     EIVSSDVVGMTYGSLFDATQTRVMSVGDRQLVKVAAWYDNEMSYTAQLVRTLAYLAEL
                     SK"
     gene            9927..11117
                     /gene="pgk"
                     /locus_tag="ACGEXS_000730"
     CDS             9927..11117
                     /gene="pgk"
                     /locus_tag="ACGEXS_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFF8011768.1"
                     /translation="MAKKIVSDLDLKGKTVLVRADFNVPLKDGEITNDNRIVQALPTI
                     QYIIEQGGKIVLFSHLGKVKEESDKAKLTLRPVAEDLSKKLDKEVVFVPETRGEKLEA
                     AIKDLKEGDVLLVENTRYEDLDGKKESKNDPELGKYWASLGDVFVNDAFGTAHREHAS
                     NVGISTHLETAAGFLMDKEIKFIGGVVNDPHKPVVAILGGAKVSDKINVIKNLVNIAD
                     KIIIGGGMAYTFLKAQGKEIGISLLEEDKIDFAKDLLEKHGDKIVLPVDTKVAKEFSN
                     DAKITVVPSDSIPADQEGMDIGPNTVKLFADELEGAHTVVWNGPMGVFEFSNFAQGTI
                     GVCKAIANLKDAITIIGGGDSAAAAISLGFENDFTHISTGGGASLEYLEGKELPGIKA
                     INNK"
     gene            11239..12000
                     /gene="tpiA"
                     /locus_tag="ACGEXS_000731"
     CDS             11239..12000
                     /gene="tpiA"
                     /locus_tag="ACGEXS_000731"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFF8011769.1"
                     /translation="MRTPIIAGNWKMNKTVQEAKDFVNALPTLPDSKEVESVICAPAI
                     QLDALTTAVKEGKAQGLEIGAQNTYFEDNGAFTGETSPVALADLGVKYVVIGHSERRE
                     LFHETDEEINKKAHAIFKHGMTPIICVGETDEERESGKANDVVGEQVKKAVAGLSEDQ
                     LKSVVIAYEPIWAIGTGKSSTSEDANEMCAFVRQTIADLSSKEVSEATRIQYGGSVKP
                     NNIKEYMAQTDIDGALVGGASLKVEDFVQLLEGAK"
     gene            12003..13520
                     /gene="gpmI"
                     /locus_tag="ACGEXS_000732"
     CDS             12003..13520
                     /gene="gpmI"
                     /locus_tag="ACGEXS_000732"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFF8011770.1"
                     /translation="MAKKPTALIILDGFANRESEHGNAVKLANKPNFDRYYNKYPTTQ
                     IEASGLDVGLPEGQMGNSEVGHMNIGAGRIVYQSLTRINKSIEDGDFFENDVLNNAIA
                     HVNSHDSALHIFGLLSDGGVHSHYKHLFALLELAKKQGVEKVYVHAFLDGRDVDQKSA
                     LKYIEETEAKFNELGIGQFASVSGRYYAMDRDKRWEREEKAYNAIRNFDAPTYATAKE
                     GVEASYNEGLTDEFVVPFIVENQNDGVNDGDAVIFYNFRPDRAAQLSEIFANRAFEGF
                     KVEQVKDLFYATFTKYNDNIDAAIVFEKVDLNNTIGEIAQNNNLTQLRIAETEKYPHV
                     TYFMSGGRNEEFKGERRRLIDSPKVATYDLKPEMSAYEVKDALLEELNKGDLDLIILN
                     FANPDMVGHSGMLEPTIKAIEAVDECLGEVVDKILDMDGYAIITADHGNSDQVLTDDD
                     QPMTTHTTNPVPVIVTKEGVTLRETGRLGDLAPTLLDLLNVEQPEDMTGESLIKH"
     gene            13650..14954
                     /gene="eno"
                     /locus_tag="ACGEXS_000733"
     CDS             13650..14954
                     /gene="eno"
                     /locus_tag="ACGEXS_000733"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017466665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-displayed alpha-enolase"
                     /protein_id="HFF8011771.1"
                     /translation="MPIITDVYAREVLDSRGNPTVEVEVLTESGAFGRALVPSGASTG
                     EHEAVELRDGDKSRYLGKGVTKAVENVNEIIAPEIIEGEFSVLDQVSIDKMMIALDGT
                     PNKGKLGANAILGVSIAVARAAADLLGQPLYKYLGGFNGKQLPVPMMNIVNGGSHSDA
                     PIAFQEFMILPVGATTFKESLRWGTEIFHNLKSILSKRGLETAVGDEGGFAPKFEGTE
                     DAVETIIQAIEAAGYKPGEEVFLGFDCASSEFYENGVYDYSKFEGEHGAKRTAAEQVD
                     YLEQLVDKYPIITIEDGMDENDWDDWKQLTERIGDRVQLVGDDLFVTNTEILAKGIEN
                     GIGNSILIKVNQIGTLTETFDAIEMAQKAGYTAVVSHRSGETEDTTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDELFETAKYDGIKSFYNLDK"
     gene            15294..15752
                     /locus_tag="ACGEXS_000734"
     CDS             15294..15752
                     /locus_tag="ACGEXS_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011772.1"
                     /translation="MADRTNKEIKTGRFIATASIVFSILLIIHYFVSLDNATAKALLN
                     LTNQNTSDKAIDYILNSFRFTGIMYILAYLAGFITFWNRHTYVWWFMFAVYVSNSLFT
                     LINLSITIQAIKAAHGAYLTLPILIVIIGSVALAIYMLVVSIKRKSTFNR"
     gene            15819..16052
                     /gene="secG"
                     /locus_tag="ACGEXS_000735"
     CDS             15819..16052
                     /gene="secG"
                     /locus_tag="ACGEXS_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFF8011773.1"
                     /translation="MHTFLIVLLIIDCIALITVVLLQEGKSSGLSGAISGGAEQLFGK
                     QKQRGVDLFLNRLTIILSILFFVLMICISYLGM"
     gene            16168..16908
                     /locus_tag="ACGEXS_000736"
     CDS             16168..16908
                     /locus_tag="ACGEXS_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFF8011774.1"
                     /translation="MQIKLPKPFFFEEGKRAVLLLHGFTGNSSDVRQLGRFLQKKGYT
                     SYAPQYEGHAAPPDEILKSSPFVWFKDALDGYDYLVEQGYDEIVVAGLSLGGDFALKL
                     SLNRDVKGIVTMCAPMGGKTEGAIYEGFLEYARNFKKYEGKDQETIDNEMDHFKPTET
                     LKELSEALDTIKEQVDEVLDPILVIQAENDNMIDPQSANYIYDHVDSDDKNIKWYSES
                     GHVITIDKEKEQVFEDIYQFLESLDWSE"
     gene            16942..19314
                     /gene="rnr"
                     /locus_tag="ACGEXS_000737"
     CDS             16942..19314
                     /gene="rnr"
                     /locus_tag="ACGEXS_000737"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFF8011775.1"
                     /translation="MNLKQSIEEIINQPEYEPMSVSDFQDALGLSSADSFRDLIKVLV
                     ELEQSGLIERTKTDRYQKKHSYRGQSKLIKGTLSQNKKGFAFLRPEDEDMEDIFIPPT
                     KINRALDGDTVIVEIHQSKGEHKGKIEGEVKSIEKHSVTQVVGTYSEARHFGFVIPDD
                     KRIMQDIFIPKGQSLGAVDGHKVLVQITKYADGSDNPEGHISAILGHKNDPGVDILSI
                     IYQHGIEIEFPDEVLQEAEAVPDHIENTEIKGRHDLRDELTITIDGADAKDLDDAISV
                     KKLANGNTQLTVSIADVSYYVTEGSALDKEAYDRATSVYLVDRVIPMIPHRLSNGICS
                     LNPNVDRLTLSCRMEIDASGRVVKHEIFDSVIHSDYRMTYDAVNQIITEKDPNIREQY
                     NEITPMLDLAQDLSNRLIQMRKRRGEIDFDISEAKVLVNEDGIPTDVQLRQRGEGERL
                     IESFMLIANETVAEHFSKLDVPFIYRVHEQPKSDRLRQFFDFITNFGIMIKGTGEDIH
                     PTTLQKVQEEVEGRPEQMVISTMMLRSMQQAHYDDVNLGHFGLSAEYYTHFTSPIRRY
                     PDLTVHRLIRKYLIEKSMDNKEVKRWEDKLPELAEHTSKRERRAIEAERDTDELKKAE
                     YMIQHIGDEFEGIVSSVANFGMFIELPNTIEGMVHIANMTDDYYRFEERQMALIGERQ
                     AKVFRIGDTVKVKVTHVDVDERLIDFQIVGMPLPKNDRSQRPARGKTIQAKTRGKSLD
                     KSKSDDKGRKKKGKQRKGKNQRNNDKSGNSKHKPFYKDKSVKKKARRKKK"
     gene            19336..19800
                     /gene="smpB"
                     /locus_tag="ACGEXS_000738"
     CDS             19336..19800
                     /gene="smpB"
                     /locus_tag="ACGEXS_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFF8011776.1"
                     /translation="MAKKKSPGTLAENRKARHDYNIEDTIEAGIVLQGTEIKSIRRGS
                     ANLKDSYAQVKNGEMYLNNMHIAPYEEGNRFNHDPLRSRKLLLHKREIIKLGDQTREI
                     GYSIVPLKLYLKHGHCKVLLGVARGKKKYDKRQALKEKAVKRDVARDMKARY"
     gene            19891..20252
                     /gene="ssrA"
                     /locus_tag="ACGEXS_000739"
     tmRNA           19891..20252
                     /gene="ssrA"
                     /locus_tag="ACGEXS_000739"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(21122..21445)
                     /locus_tag="ACGEXS_000740"
     CDS             complement(21122..21445)
                     /locus_tag="ACGEXS_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011777.1"
                     /translation="MNNEELEMRLLLMKQSIEQLQEELAPNLKTRDLVLLRYMYSYKE
                     INMLDSYLFQLATNKEQITKKQFKTKLENIREVPEIPIRQVNDILEGYKNSELYVELI
                     NSILK"
     gene            complement(21461..21607)
                     /locus_tag="ACGEXS_000741"
     CDS             complement(21461..21607)
                     /locus_tag="ACGEXS_000741"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011778.1"
                     /translation="MNNALKQEEATWGNVQGQVSQALMGTGIKDSTARSIGFWVSQVG
                     QALI"
     gene            complement(21799..22526)
                     /locus_tag="ACGEXS_000742"
                     /pseudo
     CDS             complement(21799..22526)
                     /locus_tag="ACGEXS_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5067 domain-containing protein"
BASE COUNT         7927 a         3336 c         4224 g         7108 t
ORIGIN      
        1 catgagtaaa actcatgcat aagaaatact aatttctata gaaaaagtat tactttatcg
       61 ttgtcccacc ccaacttgca cattattgta agctgacttt ccgccagctt ctgtgttggg
      121 gccccgccaa cttgcattgt ctgtagaaat tgggaatcca atttctctat gttggggccc
      181 acaccccaac tcgcattgcc tgtagaattt cttttcgaaa ttctctgtgt tggggcccct
      241 gactagagtt gaaaaaagct tgttgcaagc gcattttcat tcagtcaact actagcaata
      301 taatattata gaccctagga cattgattta tgtcccaagc tccttttaaa tgatgtatat
      361 ttttagaaat ttaatctaga catagttgga aataaatata aaacatcgtt gcttaatttt
      421 gtcatagaac atttaaatta acatcatgaa attcgttttg gcggtgaaaa aataatggat
      481 aataatgaaa aagaaaaaag taaaagtgaa ctattagttg taacaggttt atctggcgca
      541 ggtaaatctt tggttattca atgtttagaa gacatgggat atttttgtgt agataatcta
      601 ccaccagtgt tattgcctaa atttgtagag ttgatggaac aaggaaatcc atccttaaga
      661 aaagtggcaa ttgcaattga tttaagaggt aaggaactat ttaattcatt agttgcagta
      721 gtggataaag tcaaaagtga aagtgacgtc atcattgatg ttatgttttt agaagcaagt
      781 actgaaaaat taatttcaag atataaggaa acgcgtcgtg cacatccttt gatggaacaa
      841 ggtaaaagat cgttaatcaa tgcaattaat gatgagcgag agcatttgtc tcaaattaga
      901 agtatagcta attttgttat agatactaca aagttatcac ctaaagaatt aaaagaacgc
      961 attcgtcgat actatgaaga tgaagagttt gaaactttta caattaatgt cacaagtttc
     1021 ggttttaaac atgggattca gatggatgca gatttagtat ttgatgtacg atttttacca
     1081 aatccatatt atgtagtaga tttaagacct ttaacaggat tagataaaga cgtttataat
     1141 tatgttatga aatggaaaga gacggagatt ttctttgaaa aattaactga tttgttagat
     1201 tttatgatac ccgggtataa aaaagaaggg aaatctcaat tagtaattgc catcggttgt
     1261 acgggtggac aacatcgatc tgtagcatta gcagaacgac taggtaatta tctaaatgaa
     1321 gtatttgaat ataatgttta tgtgcatcat agggacgcac atattgaaag tggcgagaaa
     1381 aaatgagaca aataaaagtt gtacttatcg gtggtggcac tggcttatca gttatggcta
     1441 ggggattaag agaattccca attgatatta cggcgattgt aacagttgct gataatggtg
     1501 ggagtacagg gaaaatcaga gatgaaatgg atataccagc accaggagac atcagaaatg
     1561 tgattgcagc tttaagtgat tctgagtcag ttttaagcca actttttcag tatcgctttg
     1621 aagaaaatca aattagcggt cactcattag gtaatttatt aatcgcaggt atgactaata
     1681 ttacgaatga tttcggacat gccattaaag cattaagtaa aattttaaat attaaaggta
     1741 gagtcattcc atctacaaat acaagtgtgc aattaaatgc tgttatggaa gatggagaaa
     1801 ttgtttttgg agaaacaaat attcctaaaa aacataaaaa aattgatcgt gtgtttttag
     1861 aacctaacga tgtgcaacca atggaagaag caatcgatgc tttaagggaa gcagatttaa
     1921 tcgttcttgg accagggtca ttatatacga gcgttatttc taacttatgt gtgaatggta
     1981 tttcagatgc gttaattcat tctgatgcgc ctaagctata tgtttctaat gtgatgacgc
     2041 aacctgggga aacagatggt tatagcgtga aagatcatat cgatgcgatt catagacaag
     2101 ctggacaacc gtttattgat tatgtcattt gtagtacaca aactttcaat gctcaagttt
     2161 tgaaaaaata tgaagaaaaa cattctaaac cagttgaagt taataaggct gaacttgaaa
     2221 aagaaagcat aaatgtaaaa acatcttcaa atttagttga aatttctgaa aatcatttag
     2281 taagacataa tactaaagtg ttatcgacaa tgatttatga catagcttta gaattaatta
     2341 gtactattcc tttcgtacca agtgataaac gtaaataata tagaacgtaa tcatattatg
     2401 atatgataat agagctgtga aaaaaatgaa aatagacagt ggttctaagg tgaatcatgt
     2461 tttaaataag aaaggaatga ctgtacgatg agctttgcat cagaaatgaa aaatgaatta
     2521 actagaatag acgtcgatga aatgaatgca aaagcagagc tcagtgcact gattcgaatg
     2581 aatggtgcac ttagtctttc aaatcaacaa tttgttataa atgttcaaac ggaaaatgca
     2641 acaacggcaa gacgtattta ttcgttgatt aaacgtgtct ttaatgtgga agttgaaata
     2701 ttagtccgta aaaaaatgaa acttaaaaaa aataatattt atatttgtcg tacaaagatg
     2761 aaagcgaaag aaattcttga tgaattagga attttaaaag acggcatttt tacgcatgaa
     2821 attgatcatt caatgattca agatgacgaa atgagacgca gttacttgag aggagctttt
     2881 ctggcaggtg gctcagtgaa taaccctgaa acatcttcgt accatttgga aattttttct
     2941 caaaatgaga gtcatgcaga aggcttaacg aaactaatga atagttatga gttgaatgcc
     3001 aaacatttag agcgaaaaaa aggaagtatt acgtatttaa aagaagcgga aaagatttcg
     3061 gattttctta gtttgatagg tggctatcaa gcgttattaa aatttgaaga cgtacgtatt
     3121 gtaagagata tgcgtaattc tgttaaccga ctcgttaatt gtgaaacggc caatctaaat
     3181 aaaacagtta gtgctgcgat gaaacaagtt gagagcatta aattgattga taaagaaatt
     3241 ggtattgaaa atttaccaga caggttgaga gagattgcta gaattcgagt agaacatcaa
     3301 gaaatttcgt tgaaagagct tggagaaatg gtatcaactg gtccaatttc aaaatcaggt
     3361 gtaaatcacc gattaagaaa actgaatgat ttagccgata agattagaaa tggtgaacaa
     3421 atagaattat aagtaagaag gtgtttttgg tagtgaatta tcaaaaacac ttttttatga
     3481 ttaaaaagtg tttaatgaat aatagtcgac taactattaa attgagctgg aaagtttaat
     3541 gaaggaaatt caataaccaa gtgatgccta attttctcat taaatagcat catatgtatc
     3601 aatttggtca taagaaaata tgaatgtgat aattaattga gtccctgaaa gtccctgatc
     3661 atagacaaaa acaaaatcag cataaataag aatcccgaca ttgcgggatt ctctgtattg
     3721 aaaagtattg tattttatta aaacagcctc cttgaaggga attgaacccc tatcttaaga
     3781 accggaatct tacgtgttat ccattacacc acaaggagca aatatgtaat catatctctg
     3841 tactatgaga agtaaaataa gtacaatcta aaatagatat tatcagttta caaaggaaag
     3901 agaaaagcgt caaacaatgt aactatttaa agtcaaagtg tttgaccaaa tttgacttaa
     3961 tatgtaaaat aatgagtaac agttattaca aggaggaaat atagatgaat ttaattccta
     4021 cagttattga aacaacaaac cgcggtgaac gtgcatatga tatatactca cgtttattaa
     4081 aagaccgtat tattatgtta ggttcacaaa ttgatgacaa cgtagcaaat tcaatcgtat
     4141 cacagttatt attcttacaa gcgcaagact cagagaaaga tatttattta tacattaatt
     4201 caccaggtgg aagtgtaaca gctggttttg cgatttatga tacaattcaa cacattaaac
     4261 ctgatgttca aacaatttgt atcggtatgg ctgcatcaat gggatcattc ttattagcag
     4321 ctggtgcaaa aggtaaacgt ttcgcgttac caaatgcaga agtaatgatt caccaaccat
     4381 taggtggtgc tcaaggacaa gcaactgaaa tcgaaattgc tgcaaatcac attttaaaaa
     4441 cacgtgaaaa attaaaccgc attttatcag agcgtactgg tcaaagtatt gaaaaaatac
     4501 aaaaagacac agatcgtgat aacttcttaa ctgcagaaga agctaaagaa tatggcttaa
     4561 ttgatgaagt gatggtacct gaaacaaaat aattcaaagt aaagagtaga ctaagctgtc
     4621 tgctcttttt gtatgagtaa accaaggtgt caataatttg tttactatac tttgagcgga
     4681 aatatgattg aatgaagcta gttgaaccgt aactatatga aatgttccct tcaaagtaga
     4741 cattgaaagg aacatttcaa tcctttgttt gtaagtcgct ctagacatta catttagtac
     4801 atatgttgtt tctaatgctc attaatggta ttgattattc tttaattaaa tcttcaagtg
     4861 ccatttttaa attactatat ttaaattgga atcccaatgc ttgaatttta ttaggtaata
     4921 ctttttgagt atccaatact actgttgaca tttgaccaag tatgagacgc attgcaagac
     4981 ttggtgccca agtttcatga ggcttatgca tagctcttgc taaagtgtag ccaaataaat
     5041 tttgacgttc aggtataggt gcagttaaat taaacggacc actagctgac tcgttattta
     5101 ttaaaaataa aatagcttga attaaatcat tgatatgaat ccatgaatac cattgttgac
     5161 cagaacctaa tttaccacca atgtaatatt cgtatggtag tttcattgtt tgtaacgcac
     5221 cgccttcatt cgataaaatt ataccgaaac gaccgatgac aactcgtgta cctaattgtt
     5281 caaattgttg tgcgaaacgt tcccattgat acacaatatc tgataagaaa tcaaaaggta
     5341 aagttttata aacttctgtg taactcataa ataaatcagg aggatagtaa ccagtggcac
     5401 tagcattaaa taaagcttta ggtgctttat tacgtgattt aaacaattca tataaagctt
     5461 gcgtagattg aattctactt agcattagcg tttgtttata ttccggtgtc catcgtttat
     5521 tcaatgtagc acctgctaag ttgatgacca catcgatatt ttgaggaact ttgtgttccc
     5581 acccagattt agcccagttg acatatgaaa ttttcttatc atttgaaatt tggtcgtgtc
     5641 gcgttaatat cgtgatatgt gaatctgatt ttttaatttc attaactaat tgagatccaa
     5701 ccataccagt cccaccagta attaagtatt gtttcattat cattcacccc atgtaatttt
     5761 gtatttagtt actatttaat aatcattatt gttgttcaaa ggttatacat tatttagaca
     5821 ataatatgtc aataactttt tttgaattgt attttaataa aaatgatata agttataata
     5881 aaggtgtacc ttcgataacg aataaacatc tcttaaaagt atgtgtaaaa cgctgcatga
     5941 tacaaacgaa ggtaaaaatt tgactccctt tagtagtgga cccgtacgtt aatcgtgcgg
     6001 gtcgttttta tttatttctt attcccatta tacatcaatt taaagcatta attttttaaa
     6061 caaatttaag aatacatagt aatataacaa tctaaacata aaaactttta acacaacact
     6121 taaaccaatg ctttaatttt caatacgtag ctataatttg ttgtaaaatc aaaaaggtta
     6181 aaatgttaat tttcaaaaaa aggctcaaaa tatgtttgat ttagttatta aatgttaaga
     6241 tatataagac tactatttct ttgtaaaaat gaatccgatt tacgagtgag taatagtgaa
     6301 ggcagtttta agttgaagaa ggcaaaaaga gtaaatgttt attaatattt gtagaaacta
     6361 ggtaagcaaa ttagttgtga aaatgttaat ggttgcgtga taatttctat atttaaatta
     6421 gtttgaagtg agggagagta tgtcgaatca aaattacgac tacaataaaa atgaagatgg
     6481 aagtaagaag aaaatgagta caacagcgaa agtagttagc attgcgacgg tattgctatt
     6541 actcggagga ttagtatttg caatttttgc atatgtagat cattcgaata aagctaaaga
     6601 acgtatgttg aacgaacaaa agcaggaaca aaaagaaaag cgtcaaaaag aaaatgcaga
     6661 aaaagagaga aagaaaaagc aacaagagga aaaagagcag aatgagctag attcacaagc
     6721 aaaccaatat cagcaattgc cacagcagaa tcaatatcaa tatgtgccac ctcagcaaca
     6781 agcacctaca aagcaacgtc ctgctaaaga agagaatgat gataaagcat caaaggatga
     6841 gtcgaaagat aaggatgaca aagcatctca agataaatca gatgataatc agaagaaaac
     6901 tgatgataat aaacaaccag ctcagcctaa accacagccg caacaaccaa caccaaagcc
     6961 aaataataat caacaaaaca atcaatcaaa tcagcaagca aaaccacaag caccacaaca
     7021 aaatagccaa tcaacaacaa ataaacaaaa taatgctaat gataagtagt atttagtcaa
     7081 acaaaaatga accagtatga cagacaacac aattaattag gttgtctcga atattggttc
     7141 ttatttttat aattgttaat taggggagag atgatactta aatagttagt tgtttatttt
     7201 acggatagtg aaatttattt tgagtgaggt gggacagaaa tgatattttc gcaaaattta
     7261 tttcgtcgtc ccaccccaac ttccattgcc tgtagaaatt ggaaatccaa tttctctatg
     7321 ttggggcccc tgactttaat tgaaaaaagc ttgttacaag tgcattttcg ttcggttaac
     7381 tactactaat gtgacttttt ggattctaga gcattgattt atgtcctagt ctcaaataat
     7441 aagcaaatga ttatcgagtc agtataaggg tatacatttg actacagcga ataaaataaa
     7501 acgttttgtt gaataacaaa tcgaaaatat attgcaagcg ctttatcaaa ttatttagaa
     7561 aatttaagtt ttatgcttgc aatttttgaa atagaatagt actattgcaa gtgtaaagag
     7621 gttaattttt gtcccgcgcg ggacttaaaa aggcaaccac tggttgtgac atatccttat
     7681 ttacatttat aaatataagg aggaggtagt agtgaaagac ttattgcaag cacagcaaaa
     7741 gcttataccg gatctcatag ataaaatgta taaacgtttt tctattctta ctactatctc
     7801 aaaaaatcag cctgtcggac gtcgaagttt aagcgaacat atggatatga ctgaacgtgt
     7861 actgcgttct gaaacagata tgcttaagaa acaagatttg ataaaagtta agcctaccgg
     7921 aatggaaatt acagctgaag gtgagcaact gatttcgcaa ttgaaaggtt actttgatat
     7981 ctatgcagat gataatcgtc tgtcagaagg tattaagaat aaatttcaaa ttaaggaagt
     8041 tcatgttgtt cctggtgatg ctgataatag tcaatctgtt aaaacagaat taggtagaca
     8101 agcaggtcaa ttacttgaag gcatattaca agaagacgcg atagttgctg taactggcgg
     8161 atccacgatg gcatgtgtta gtgaagcaat tcatttatta ccatataatg tattcttcgt
     8221 accagccaga ggtggactag gcgaaaatgt tgtctttcag gcaaacacaa ttgcagccag
     8281 tatggcacaa caagctggcg gttattatac gacgatgtat gtacctgata atgtcagtga
     8341 aacaacatat aatacattgt tgttagagcc atcagtcata aacactttag acaaaattaa
     8401 acaagcaaac gttatattac acggcattgg tgatgcgctg aagatggcgc atcgacgtca
     8461 atcacctgaa aaggtcattg aacaacttca acatcatcaa gctgtcggag aggcatttgg
     8521 ttattatttt gatacacaag gtcaaattgt ccataaggtt aaaacaattg gacttcaatt
     8581 agaagacctt gaatcaaaag actttatttt tgcagttgca ggaggcaaat cgaaaggtga
     8641 agcaattaaa gcatacttga cgattgcacc caagaataca gtgttaatca ctgatgaagc
     8701 cgcagcaaag ataatacttg aataagagat aaaaagttta atacttttta aatatcattt
     8761 taaaggaggc cattataatg gcagtaaaag tagcaattaa tggttttggt agaattggtc
     8821 gtttagcatt cagaagaatt caagaagtag aaggtcttga agttgtagca gtaaacgact
     8881 taacagatga cgacatgtta gcgcatttat taaaatatga cactatgcaa ggtcgtttca
     8941 caggtgaagt agaggtagtt gatggtggtt tccgcgtaaa tggtaaagaa gttaaatcat
     9001 tcagtgaacc agatgcaagc aaattacctt ggaaagactt aaatatcgat gtagtattag
     9061 aatgtactgg tttctacact gataaagata aagcacaagc tcatattgaa gcaggcgcta
     9121 aaaaagtatt aatctcagca ccagctactg gtgacttaaa aacaatcgta ttcaacacta
     9181 accaccaaga gttagacggt tctgaaacag ttgtttcagg tgcttcatgt actacaaact
     9241 cattagcacc agttgctaaa gttttaaacg atgactttgg tttagttgaa ggtttaatga
     9301 ctacaattca cgcttacaca ggtgatcaaa atacacaaga cgcacctcac agaaaaggtg
     9361 acaaacgtcg tgctcgtgca gcggcagaaa acatcatccc taactcaaca ggtgctgcta
     9421 aagctatcgg taaagttatt cctgaaatcg atggtaaatt agatggtggt gcacaacgtg
     9481 ttcctgtagc tacaggttca ttaactgaat taacagtagt attagaaaaa caagacgtaa
     9541 cagttgaaca agttaacgaa gctatgaaaa atgcttcaaa cgaatcattc ggttacactg
     9601 aagacgaaat cgtttcttca gacgttgtag gtatgactta cggttcatta ttcgacgcta
     9661 cacaaactcg tgtaatgtca gttggcgacc gtcaattagt taaagttgca gcttggtatg
     9721 ataacgaaat gtcatatact gcacaattag ttcgtacatt agcatactta gctgaacttt
     9781 ctaaataatt ttagtatagt ttttattcaa atacgctagt gctcagaact atttagcatt
     9841 aattaaagct tatgagtaag cggggagcac aaacgcttct ccgcttattt ttatataaaa
     9901 tttcctaatt acaaggagga aacaccatgg ctaaaaaaat tgtttctgat ttagatctta
     9961 aaggtaaaac agtcctagta cgtgctgatt ttaacgtacc tttaaaagac ggtgaaatta
    10021 ctaatgacaa ccgtatcgtt caagctttac ctacaattca atacatcatc gaacaaggtg
    10081 gtaaaatcgt actattttca catttaggta aagtgaaaga agaaagtgat aaagcaaaat
    10141 taactttacg tccagttgct gaagacttat ctaagaaatt agataaagaa gttgttttcg
    10201 taccagaaac acgcggcgaa aaacttgaag ctgctattaa agaccttaaa gaaggcgacg
    10261 tattattagt tgaaaataca cgttatgaag atttagacgg taaaaaagaa tctaaaaatg
    10321 atccagaatt aggtaaatac tgggcatctt taggtgatgt gtttgtaaat gatgcttttg
    10381 gtactgcgca tcgtgagcat gcatctaatg ttggtatttc tacacattta gaaactgcag
    10441 ctggattctt aatggataaa gaaattaagt ttattggcgg cgtagttaac gatccacata
    10501 aaccagttgt tgctatttta ggtggagcaa aagtatctga caaaattaat gtcatcaaaa
    10561 acttagttaa catagctgat aaaattatca tcggcggagg tatggcttat actttcttaa
    10621 aagcgcaagg taaagaaatt ggtatttcat tattagaaga agataaaatc gacttcgcaa
    10681 aagatttatt agaaaaacat ggtgataaaa ttgtattacc agtagacact aaagttgcta
    10741 aagaattttc taatgatgcc aaaatcactg tagtaccatc tgattcaatt ccagcagacc
    10801 aagaaggtat ggatattgga ccaaacactg taaaattatt tgcagatgaa ttagaaggtg
    10861 cgcacactgt tgtatggaat ggacctatgg gtgtattcga gttcagtaac tttgcacaag
    10921 gtacaattgg tgtatgtaaa gcaattgcaa accttaaaga tgcaattacg attatcggtg
    10981 gcggtgattc agctgcagca gcaatctctt taggttttga aaatgacttc actcatattt
    11041 caactggtgg cggcgcgtca ttagagtacc tagaaggtaa agaattgcct ggtatcaaag
    11101 caatcaataa taaataataa agtgatagtt taaagtgatg tggcatgttt gtttaacatt
    11161 gttacgggaa aacagtcaca agatgacatc gtgtttcatc acttttcaaa aatatttaca
    11221 aaacaaggag tgtctttaat gagaacacca attatagctg gtaactggaa aatgaacaaa
    11281 acagtacaag aagcaaaaga cttcgtcaat gcattaccaa cattaccaga ttcaaaagaa
    11341 gtagaatcag taatttgtgc accagcaatt caattagatg cattaactac tgcagttaaa
    11401 gaaggaaaag cacaaggttt agaaatcggt gctcaaaata cgtatttcga agataatggt
    11461 gcgttcacag gtgaaacgtc tccagttgca ttagcagatt taggcgttaa atacgttgtt
    11521 atcggtcatt ctgaacgtcg tgaattattc cacgaaacag atgaagaaat taacaaaaaa
    11581 gcgcacgcta ttttcaaaca tggaatgact ccaattatat gtgttggtga aacagacgaa
    11641 gagcgtgaaa gtggtaaagc taacgatgtt gtaggtgagc aagttaagaa agctgttgca
    11701 ggtttatctg aagatcaact taaatcagtt gtaattgctt atgaaccaat ctgggcaatc
    11761 ggaactggta aatcatcaac atctgaagat gcaaatgaaa tgtgtgcatt tgtacgtcaa
    11821 actattgctg acttatcaag caaagaagta tcagaagcaa ctcgtattca atatggtggt
    11881 agtgttaaac ctaacaacat taaagaatac atggcacaaa ctgatattga tggggcatta
    11941 gtaggtggcg catcacttaa agttgaagat ttcgtacaat tgttagaagg tgcaaaataa
    12001 tcatggctaa gaaaccaact gcgttaatta ttttagatgg ttttgcgaac cgcgaaagcg
    12061 aacatggtaa tgcggtaaaa ttagcaaaca agcctaattt tgatcgttat tacaacaaat
    12121 atccaacgac tcaaatcgaa gcgagtggct tagatgttgg actacctgaa ggacaaatgg
    12181 gtaactcaga agttggtcat atgaatatcg gtgcaggacg tatcgtttat caaagtttaa
    12241 ctcgaatcaa taaatcaatt gaagacggtg atttctttga aaatgatgtt ttaaataatg
    12301 caattgcaca cgtgaattca catgattcag cgttacacat ctttggttta ttgtctgacg
    12361 gtggtgtaca cagtcattac aaacatttat ttgctttgtt agaacttgct aaaaaacaag
    12421 gtgttgaaaa agtttacgta cacgcatttt tagatggccg tgacgtagat caaaaatccg
    12481 ctttgaaata catcgaagag actgaagcta aattcaatga attaggcatt ggtcaatttg
    12541 catctgtgtc tggtcgttat tatgcaatgg atcgtgacaa acgttgggaa cgtgaagaaa
    12601 aagcttacaa tgctattcgt aattttgatg ccccaactta tgcaactgcc aaagaaggtg
    12661 tagaagcaag ctataatgag ggcttaactg acgaattcgt agtaccattc atcgttgaga
    12721 atcaaaatga cggtgttaat gatggagatg cagtgatctt ctataatttc cgacctgata
    12781 gagcagcgca attatcggaa atttttgcga acagagcatt cgaaggcttt aaagttgaac
    12841 aagttaaaga cttattctat gcaacattca ctaagtataa tgacaatatc gatgcggcta
    12901 tcgtcttcga aaaagttgat ttaaataata caattggtga aattgcacaa aataacaatt
    12961 taactcaatt acgtattgca gaaactgaaa aataccctca cgttacttac tttatgagtg
    13021 gtggacgtaa cgaggaattt aaaggtgaac gccgtcgttt aattgattca cctaaagttg
    13081 caacgtatga cttgaaacca gaaatgagtg cttatgaagt taaagatgca ttattagaag
    13141 agttaaataa aggtgacttg gacttaatta ttttaaactt tgctaaccct gatatggttg
    13201 gacatagtgg tatgcttgag ccgacaatca aagcaatcga agcggttgat gaatgtttag
    13261 gagaagtggt tgataagatt ttagacatgg acggttatgc aattattact gctgaccatg
    13321 gtaactctga tcaagtattg acggatgatg atcaaccaat gactacgcat acaacgaacc
    13381 cagtaccagt gattgtaaca aaagaaggcg ttacacttag agaaactggt cgcttaggtg
    13441 acttagcacc tacattatta gatttattaa atgtagaaca acctgaagat atgacaggtg
    13501 aatctttaat taaacactaa tattgtaaaa gatgttaagt aaacgcttaa tgacacttat
    13561 tttttgaaaa taatagtaat atcattttgt taaatgaaag aataaagcta taataattat
    13621 agaataacta tttaaaggag attataaaca tgccaattat tacagatgtt tacgctcgcg
    13681 aagtcttaga ctctcgtggt aacccaactg ttgaagtaga agtattaact gaaagtggcg
    13741 catttggtcg tgcattagta ccatcaggtg cttcaactgg tgaacacgaa gctgttgaat
    13801 tacgtgatgg agacaaatca cgttatttag gtaaaggtgt tactaaagca gttgaaaacg
    13861 ttaatgaaat catcgcacca gaaattattg aaggtgaatt ttcagtatta gatcaagtat
    13921 ctattgataa aatgatgatc gcattagacg gtactccaaa caaaggtaaa ttaggtgcaa
    13981 atgctatttt aggtgtatct atcgcagtag cacgtgcagc agctgactta ttaggtcaac
    14041 cactttacaa atatttaggt ggatttaatg gtaagcagtt accagtacca atgatgaaca
    14101 tcgttaatgg tggttctcac tcagatgctc caattgcatt ccaagaattc atgattttac
    14161 ctgtaggtgc tacaacgttc aaagaatcat tacgttgggg tactgaaatt ttccacaact
    14221 taaaatcaat tttaagcaaa cgtggtttag aaactgcagt aggtgacgaa ggtggtttcg
    14281 ctcctaaatt tgaaggtact gaagatgctg ttgaaacaat tatccaagca atcgaagcag
    14341 ctggttacaa accaggtgaa gaagtattct taggatttga ctgtgcatca tcagaattct
    14401 atgaaaatgg tgtatatgac tacagtaagt tcgaaggcga acacggtgca aaacgtacag
    14461 ctgcagaaca agttgactac ttagaacaat tagtagacaa atatcctatc attacaattg
    14521 aagacggtat ggacgaaaac gactgggatg attggaaaca acttacagaa cgtatcggtg
    14581 accgtgtaca attagtaggt gacgatttat tcgtaacaaa cactgaaatt ttagcaaaag
    14641 gtattgaaaa cggaattggt aactcaatct taattaaagt taaccaaatc ggtacattaa
    14701 ctgaaacatt tgatgcaatc gaaatggctc aaaaagctgg ttacacagca gtagtttctc
    14761 accgttcagg tgaaacagaa gatacaacaa ttgctgatat tgctgttgct acaaacgctg
    14821 gtcaaattaa aactggttca ttatcacgta ctgaccgtat tgctaaatac aatcaattat
    14881 tacgtatcga agatgaatta tttgaaactg ctaaatatga cggtatcaaa tcattctata
    14941 acttagataa ataattttct ttataatcaa atgctgacat aattttagtt gaggattatt
    15001 atgacggtat aaattaaata aagattttga gttcacgctt aaataagttc acgcttaaat
    15061 ttatagcctg ccacagagtt gagactgtgg taggtttttt attttgaagt attaatcata
    15121 acagactaat aatcatgagg taactaataa cacatattta acttgtattc ttaaactggt
    15181 ataataaatt tatgttgaaa tgaatattgt atgacagggt attcactttt attaaaaggt
    15241 aaaattaaat aaaggtttta tagaacgtat ttaaatatat gaggagtaaa caaatggctg
    15301 atagaacgaa taaagaaatt aaaacaggac gctttattgc aactgcatca atcgtattct
    15361 caatattatt gattattcat tactttgttt cgttggataa tgcgactgcc aaagcattac
    15421 ttaatttaac gaatcaaaac acttcagata aagcgattga ttacatttta aacagcttta
    15481 gattcactgg tattatgtat attttggctt atctagcagg cttcatcact ttttggaatc
    15541 gacatactta tgtgtggtgg tttatgtttg cagtttatgt atcaaatagt ttgtttacgt
    15601 tgattaattt atcaatcaca attcaagcaa taaaagctgc acacggtgcg tacttaacat
    15661 tgccaatttt aattgttatt ataggttcgg ttgcattagc gatttatatg cttgttgttt
    15721 ctatcaaacg taaaagtaca tttaatcgct agaaaattga ttttaacaat aaaaatatga
    15781 tatactactt gtcgtatata aggaacggag gacaatttat gcatacattt ttaatcgtat
    15841 tattaatcat tgattgtatt gcattaataa ctgttgtact actccaagaa ggtaaaagca
    15901 gtggactttc aggtgccatc agtggtggtg ctgagcagtt attcggtaaa caaaaacaac
    15961 gtggcgtcga tttattctta aatagattaa caattatttt atcaatatta ttttttgtac
    16021 ttatgatttg cataagttat cttggtatgt aaggtccggc gatgtaaatg tcgggctttt
    16081 ttatttataa ttaagaatgt aatagtttaa caataagcta tgtaaaatat atagcctagt
    16141 taagtatgca aagggagcgt tagatttatg cagataaaat taccaaaacc tttctttttt
    16201 gaggaaggta aacgtgccgt gttattacta catggtttta caggcaattc gtctgatgtt
    16261 cgtcaattag gtcgattttt acaaaagaaa ggttatacat catatgcacc gcaatatgaa
    16321 ggccacgcgg caccaccaga tgaaatactg aaatctagtc ctttcgtttg gtttaaagat
    16381 gcgttagatg gttatgatta tcttgttgaa caaggttatg atgaaattgt tgttgctggt
    16441 ctatcattag gtggggattt tgctttaaaa ttaagcttaa atagagatgt aaagggtatt
    16501 gtaacgatgt gtgctcctat gggtggcaaa actgaaggtg ccatttatga aggcttttta
    16561 gaatatgcac gcaattttaa aaagtatgaa ggtaaagatc aagagactat tgataatgaa
    16621 atggatcatt ttaaaccaac tgaaacttta aaagaactaa gtgaagcatt agatacgatt
    16681 aaagagcaag ttgatgaagt gttggatcct attttagtga ttcaagcaga aaacgacaat
    16741 atgattgatc cacaatccgc aaattatata tatgaccatg tagattctga tgacaaaaat
    16801 atcaagtggt acagtgaatc tggacatgtt attacgattg ataaagagaa agaacaagta
    16861 tttgaagata tttatcaatt tttagagtca ttagactggt cagaataaaa agagatttta
    16921 acattagaaa ggaggggcat aatgaattta aagcaatcta tagaagagat tattaatcaa
    16981 cctgaatatg aacctatgtc agtgtcagat tttcaagatg cattaggttt aagcagtgcc
    17041 gactcgttta gagatttaat taaggtgctt gtggagttag aacaatcagg attaatcgaa
    17101 cgtacaaaaa cagacagata ccaaaaaaag catagttata gaggtcaatc aaaattgata
    17161 aaaggaacgt taagtcaaaa taaaaaaggc tttgcattct taagacctga agatgaggat
    17221 atggaagata tatttattcc cccgacgaaa attaatcgtg ccttggatgg agatactgtt
    17281 attgtagaaa tccatcaatc aaaaggtgaa cataaaggta aaatcgaagg ggaagttaag
    17341 tcgattgaga agcattctgt aactcaagtt gttggtacgt atagtgaagc tagacatttt
    17401 ggctttgtta ttccggatga taaacgtatt atgcaagata ttttcattcc taaaggtcaa
    17461 agtttaggcg cagtcgatgg tcataaggta cttgtacaaa ttactaagta tgctgatggt
    17521 tcagataatc cagaaggaca tatttctgct attttaggac ataaaaatga tcctggcgta
    17581 gatattttat ctattatcta tcaacatggc atagaaattg aatttcctga tgaagtgtta
    17641 caagaagctg aagcagtacc tgatcatatt gaaaatactg aaattaaagg ccgtcatgat
    17701 ttacgtgatg aattgacaat cacaattgat ggtgctgatg ctaaagactt agatgacgca
    17761 attagtgtta aaaagttagc gaacggtaat acgcaattaa ctgtaagtat tgctgatgtc
    17821 agctattatg taacagaagg ttctgcattg gataaagagg catatgatag agcgacaagt
    17881 gtatatcttg ttgaccgtgt aattccaatg attccacatc gattaagtaa tggtatttgt
    17941 tcattgaatc ctaatgttga tcgtttaact ctaagctgtc gcatggaaat cgatgctagt
    18001 ggtcgcgttg ttaaacatga aatttttgat agtgttatac attctgatta tcgaatgacg
    18061 tatgatgcgg taaatcagat tattactgaa aaggatccta acattcgcga acaatataat
    18121 gaaattacgc ctatgctaga tttagcacaa gatttatcta atcgtttgat tcaaatgaga
    18181 aaacgacgtg gtgaaatcga ttttgatatt agtgaagcaa aagtattagt taacgaagac
    18241 ggtataccaa cagatgttca attaagacaa cgtggcgagg gtgaacgtct aattgaatca
    18301 tttatgttaa ttgcaaatga aacagttgct gaacatttta gtaagttaga tgtacctttt
    18361 atttaccgag tgcatgagca acctaaatca gatcgcttaa gacaattctt tgattttatt
    18421 acaaactttg gcatcatgat taagggtact ggcgaagata ttcatccaac aacacttcaa
    18481 aaggttcaag aagaagtaga aggtcgacct gaacaaatgg tcatttcaac aatgatgttg
    18541 cgttcaatgc aacaagcgca ttatgatgat gtgaacttgg gacattttgg cttatcagct
    18601 gaatattata cgcattttac atcaccaatt agacgttatc ctgatttaac agttcatcgt
    18661 ttaatccgta agtatttaat tgagaaatca atggataaca aagaagtgaa gcgttgggaa
    18721 gacaaattgc ctgagttagc tgaacatact tctaaacgtg aacgtcgtgc tattgaggca
    18781 gaacgtgata ctgatgaatt gaaaaaagca gaatatatga ttcaacatat tggtgatgaa
    18841 tttgaaggta ttgtcagctc agtagctaac ttcggtatgt tcattgaatt gccaaatacg
    18901 atagaaggta tggttcatat tgcgaatatg actgatgatt attaccgttt tgaagagcgt
    18961 caaatggcat taattggtga gcgtcaagct aaagtattta gaattggtga cacagttaag
    19021 gttaaagtga cgcatgttga tgtagatgaa cgattaattg attttcaaat tgtaggtatg
    19081 cctttaccga aaaatgatcg atcacagcgc ccagcgcgag gtaagacaat tcaagccaaa
    19141 acgcgtggta aatcattaga taaatcaaaa tctgatgata agggtcgtaa gaaaaaaggt
    19201 aagcaacgta aaggtaaaaa ccaacgtaat aatgataaat caggtaatag taagcataag
    19261 ccattttata aagataaaag tgtgaaaaag aaagcacgtc gtaagaaaaa ataagcagca
    19321 atgaggtgag tatgaatggc taagaagaaa tcaccaggta cattagcgga aaatcgtaag
    19381 gcaagacatg attataatat tgaagatacg attgaagcgg gaattgtatt gcaaggcaca
    19441 gaaataaaat caattcgccg aggtagtgct aaccttaaag atagttatgc gcaagttaaa
    19501 aacggtgaaa tgtatttgaa taatatgcat atagcaccat acgaagaagg gaatcgtttt
    19561 aatcacgatc ctcttcgttc tcgaaaatta ttattgcaca agcgtgaaat cattaaattg
    19621 ggtgatcaaa cacgtgagat tggttattcg attgtgccgt taaagcttta tttgaagcat
    19681 ggacattgta aagtattact tggtgttgca cgaggtaaga aaaaatatga taaacgtcaa
    19741 gctttgaaag aaaaagcagt caaacgagat gttgcgcgcg atatgaaagc ccgttattaa
    19801 gcgatttagt tgcttaatcg ggctatattt gatatagtta tatgtgcttt tgtaaattac
    19861 aaaagtatga tttgtttgat ttattatttc ggggacgttc atggattcga caggggtccc
    19921 ccgagctcat taagcgtgtc ggagggttgt cttcgtcatc aacacacaca gtttataata
    19981 actggcaaat caaacaataa tttcgcagta gctgcctaat cgcactctgc atcgcctaac
    20041 agcatttcct atgtgctgtt aacgcgattc aaccttaata ggatatgcta aacactgccg
    20101 tttgaagtct gtttagaaga aacttaatca aactagcatc atgttggttg tttatcactt
    20161 ttcatgatgc gaaacctatc gataaactac acacgtagaa agatgtgtat caggaccttt
    20221 ggacgcgggt tcaaatcccg ccgtctccat atttgtagcc tacagccttt gtggttgtgg
    20281 gcttttttat tttgtgtttt tcaggggata atgcattgca gaatttgttg tgagtattga
    20341 tatagcagtg tttgtatagg tgtttatttg atggaggaaa gagtaataag tgattatgaa
    20401 ttagtttttg agatataagg ggacagtgat gtgtgtcaaa taagtgtcaa aaaagttgga
    20461 ttctgagttt tacattcaac attgttcatg aagaaacttc tttatacgca aaaaattctc
    20521 catgttatat atgtcaatat aaaaatgtga atcgtctaca cttaattgga taaatggcta
    20581 ctgaaaaaga acttttcatt tttgttacgt cactaagtgg gtgtagttat aaagagatga
    20641 gccgagtttt gatattttca ttagaatcaa tatgcctatt aacacaatca gcaatagttg
    20701 acgagacgga aataaaagaa gtcgtagtta agaaatgcat ttcacaacat accattgtag
    20761 ccatttttat tgttttggat gataaactct ttttggaatt tttagttttt ataatttgca
    20821 actacactac ttcttttact aatattaatg tctaagtaat cgataaaaaa ttttccattg
    20881 aataaatgag aagttaaaaa ctttacttaa cctttctcat tgcattttcc tattcacgat
    20941 tttaagaacc caacatacta caaacgaatt ttaaaaggcg agagtaaagc ttacttgttt
    21001 attatacata tttaaaatcc aagagtcaga acagactact cctctttata actataaaaa
    21061 atagctatga aaaaatctat cgtcatagat tccttcatag ctaatcttag tatgtttatt
    21121 tttattttag gatgctattt atcaactcaa catataactc actattttta taaccttcta
    21181 atatatcatt aacttgtcta ataggtattt ctggtacttc tctaatgttt tccaattttg
    21241 ttttaaattg tttttttgtt atttgctctt tatttgtagc caattggaac aagtaagaat
    21301 ctagcatatt aatttcttta tatgaataca tatatcttaa taacactaaa tctctagttt
    21361 ttaagttagg cgctagttct tcttgtaatt gttctattga ttgtttcatt aataacaatc
    21421 tcatttctaa ttcttcatta ttcattttat cacactcttt ttatattaat gcttgaccaa
    21481 cttgggaaac ccaaaaccct atgcttcttg cagtagaatc tttaatacca gttcccatca
    21541 atgcttgtga aacttgacct tgtacatttc cccatgtagc ctcttcttgt tttaatgcat
    21601 tattcaatgc gggatttaca aatttatccc atcttttttt tatgattttc cggcacgggg
    21661 actgatttct ttaacaccat taaacacaga ttttttattt ttaatcatag ctttatagta
    21721 tcatgttggc taagctataa ataagtcagt ttctctaaaa attaaataac tgaatgtaag
    21781 acaatcaaca aaccaaattt atacttcatc taaaccactg tggtcgtcat ctttttgctt
    21841 ttctttttct ttctctcgtt cttgttcttt tttgtactct tcttcaaatt ctttttcttt
    21901 cttttctact tcttctcttg tttccgctct atgagaaaaa tcttcggttt taagtttact
    21961 aaatttgaat gatttagaat caactgtttt atcttctgag tatttatgga catttaaatt
    22021 aatatttcca tcacctctta actcatagat aaacatggct tgtgcagttt tgcctttttt
    22081 aatttgatct tggttatgtt ctgtccaatc tttatatttt ttatcactta aaagataacc
    22141 atctcttaat ttatttactg tatttttatc atcttgagtg atattaatat agtcatgaga
    22201 aatagaagat ggatttaaat ctttatcgtc ttttttagca gtaatttcca ttttaaaagc
    22261 gatatatttc tttttctcat ctttttcatt gatgataaac ggttctttta ttttagcttc
    22321 aaatttgtca ctaacaatag tatcgccttt aatttttata tccatatttt tttgctttta
    22381 aattctttaa gttcttcatt taattcttca ttgtcatttt ctttcttttt gtgactagtg
    22441 ctctcttttt ttgcactatc ttgatgatgt ccacaagcac ctaagataag tgtacttgct
    22501 aataatatcc ccattacttt tttcatttaa catgtctcct ttatttcgca aaaatttatt
    22561 ttaaaaactc taaatgactt atcattttga gtaat
//
LOCUS       DAXWFK010000024        22303 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.024, whole genome shotgun sequence.
ACCESSION   DAXWFK010000024 DAXWFK010000000
VERSION     DAXWFK010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 22303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22303
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.024"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            117..1259
                     /locus_tag="ACGEXS_000743"
     CDS             117..1259
                     /locus_tag="ACGEXS_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HFF8011779.1"
                     /translation="MSSASTQSTKTSDIHNESIDKQMEAKAHETAQNTDLKNEARSLF
                     DNATKSIGRLAGNDESLNLNLKDMLSEVFKPHTKNEADEIFIAGTAKTTPAICDISEE
                     WGKPWLFSRVFIAFTVTFIGLWVMAAIFNNTNAIPGLIFIGALTVPLSGLFFFYESNA
                     FKNISIFEVIIMFFIGGVFSLLSTMVLYRFVVFSDQFERFGSLTFFDAFLVGLVEETG
                     KALIIVYFVNKLKTNKILNGLLIGAAIGAGFAVFESAGYILNFALGENVPLLDIVFTR
                     AWTAIGGHLVWSAIVGAAIVIAKEQHGFEFKDIFDKRFLIFFLSAVVLHGIWDTSLTV
                     LGSDTLKIFILIVIVWILVFILMGAGLKQVNLLQKEFKEQQKKVDE"
     gene            complement(1573..3048)
                     /gene="nikA"
                     /locus_tag="ACGEXS_000744"
     CDS             complement(1573..3048)
                     /gene="nikA"
                     /locus_tag="ACGEXS_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFF8011780.1"
                     /translation="MKFKRLATIFSAVLVLSGCGSMHSSGKDLNISLPLKTKSIAPYE
                     TDVPVKIGAAESLFKTNDQGKIEKALVKSYHQPNDTTLDIELKDNIKFQNGQKLTAEK
                     VKSSLENSMKKSDLVKYSLPISSITAKGQKLTIKTNSAYPELVSELANPFMAIYDTDA
                     KSDVNQTPVGTGPYQIKDYKQSRKISLSNFKDYWQGKPKLDHITVTYQEDGNNRVRNL
                     ESQKDDLITDVPVNKVQDIENNQNLKVSKESGFRTSLLMYNHTNKKMTKSVREALDHI
                     IDRQGIADHIYQGYAKPATSPFNDKIPYIKEPKLTKQNIEQAKMLLAKDGYTKEHPLK
                     IKLITYDGRPELSKIAQVLQSDAKKANIEIDIKSVDDIEGYLKDRSAWDATMYSFGTI
                     PRGDTGYFFNQAYKKDGAINKGDYNNSNVDDLINQLNHTVDVKERHNISNDIIKLSSR
                     DVPNSYIAYNDQIVAANSKVKNYKVTPEGIYLIDYRTTIER"
     gene            complement(3246..3602)
                     /locus_tag="ACGEXS_000745"
     CDS             complement(3246..3602)
                     /locus_tag="ACGEXS_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFF8011781.1"
                     /translation="MTVDIGRIYDNKDNTDAIRILVDRVWPRGISKRTANLDYWLKDI
                     APSTELRQWFQHDPKLFGAFKEKYEKELRDQDAQKDAFEKLKDIVNQHNHVLLLYAAK
                     DTKHNQAVVLQQLLNT"
     gene            complement(3759..3932)
                     /locus_tag="ACGEXS_000746"
     CDS             complement(3759..3932)
                     /locus_tag="ACGEXS_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011782.1"
                     /translation="MKLNQRYVKVFALYFVSIVTANIIVKNNNLIKTLIQTIAGYTVF
                     AVGLKYLTKRKNK"
     gene            complement(3958..5103)
                     /locus_tag="ACGEXS_000747"
     CDS             complement(3958..5103)
                     /locus_tag="ACGEXS_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin domain-containing protein"
                     /protein_id="HFF8011783.1"
                     /translation="MLTEQEKDIIKQTVPLLKEKGTEITSIFYPKMFKAHPELLNMFN
                     QTNQKRGMQSSALAQAVMAAAVNIDNLSVIKPVIMPVAYKHCALQVYAEHYPIVGKNL
                     LKAIQDVTGLEENDPVIQAWAKAYGVIADVFIQIEKEIYDQMMWIGFKPFKITNIKQE
                     SEDIKSFTVETEEYDFSEFTPGQYITVDVSSDKLPYRAKRHYSIVSGEKNHLTFGVKR
                     DVTTEHEGEVSTILHDEIKEGDMINLAAPVGGFVLENTTEPQLFLGSGIGVTPLVAMY
                     EAASAKGLDTQMVQVAENEQHLPFKDNFNSIASHHDNAKLYTHLKDKQGYIGAEELQV
                     FLANKPEIYICGGTKFLQSMIEALKSLNYDMDRVHYETFIPRLSVAV"
     gene            5258..5365
                     /locus_tag="ACGEXS_000748"
     CDS             5258..5365
                     /locus_tag="ACGEXS_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011784.1"
                     /translation="MSVCTPTIERIYLTNSKNIVVFLEKSKHDNKNVKV"
     gene            5676..6629
                     /locus_tag="ACGEXS_000749"
     CDS             5676..6629
                     /locus_tag="ACGEXS_000749"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFF8011785.1"
                     /translation="MNKFKGNKVVLIGNGAVGSSYAFSLVNQSIVDELVIIDLDTEKV
                     RGDVMDLKHATPYSPTTVRVKAGEYSDCHDADLVVICAGAAQKPGETRLDLVSKNLKI
                     FKSIVGEVMASKFDGIFLVATNPVDILAYATWKFSGLPKERVIGSGTILDSARFRLLL
                     SEAFDVAPRSVDAQIIGEHGDTELPVWSHANIAGQPLKTLLEQRPEGKAQIEQIFVQT
                     RDAAYDIIQAKGATYYGVAMGLARITEAIFRNEDAVLTVSALLEGEYEEEDVYIGVPA
                     VINRNGIRNVVEIPLNDEEQSKFAHSAKTLKDIMAEAEELK"
     gene            complement(6949..8478)
                     /locus_tag="ACGEXS_000750"
     CDS             complement(6949..8478)
                     /locus_tag="ACGEXS_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFF8011786.1"
                     /translation="MKSLFEKAQQFGKSFMLPIAILPAAGLLLGIGGALSNPNTVKAY
                     PILDITLLQNIFTLMSAAGSIVFQNLPVIFAIGVAIGLSRSDKGTAGLAALLGFLIMN
                     ATMNGLLTITGTLAKDQLAQNGQGMVLGIQTVETGVFGGIITGIMTAILHNKYHKVVL
                     PPYLGFFGGSRFVPIVTAFAAIFLGVLMFFIWPSIQAGIYHVGGFVTKTGAIGTFVYG
                     FILRLLGPLGLHHIFYLPFWQTALGGTLEVKGHLVQGTQNIFFAQLGDPDVTKYYSGV
                     SRFMSGRFITMMFGLCGAALAIYHTAKPEHKKVVGGLMLSAALTSFLTGITEPLEFSF
                     LFVAPILYVIHAFFDGLAFMMADIFNITIGQTFSGGFIDFLLFGVLQGNSKTNYLYVI
                     PIGIVWFCLYYIVFRFLITKFNFKTPGREDKAAAQQVEATERAQTIVAGLGGKDNIEI
                     VDCCATRLRVTLHQNDKVDKVLLESTGAKGVIQQGTGVQVIYGPHVTVIKNEIEELLG
                     D"
     gene            8840..9775
                     /locus_tag="ACGEXS_000751"
     CDS             8840..9775
                     /locus_tag="ACGEXS_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFF8011787.1"
                     /translation="MKRKIIMDCDPGHDDAIALILAGAIDSPLEILAVTTVAGNQSVD
                     KNTTNALNVLDIMGRQDIAVAKGADRPLIKPAAFASEIHGESGLDGPKLPSTPSRQAV
                     AMPASDVIINKVMTSDTPVTIVATGPLTNVATALIREPRIAEHIESITLMGGGTFGNW
                     TPTAEFNIWVDAEAAKRVFESGITINVFGLDVTHQVLADDHVIERFESINNPVAQFVV
                     ELLQFFKKTYKTHFNMDGGPIHDACTILYLLQPELFTMVPVNIDIEHQSPLTYGTMAV
                     DLNHVTGKPANAYFATAVDVEEVWNLIDHKLRTYE"
     gene            9885..9983
                     /locus_tag="ACGEXS_000752"
     CDS             9885..9983
                     /locus_tag="ACGEXS_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011788.1"
                     /translation="MYVKNASKKHKTQKYMQKFQLLYFKRLKSQKI"
     gene            10114..12210
                     /locus_tag="ACGEXS_000753"
     CDS             10114..12210
                     /locus_tag="ACGEXS_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFF8011789.1"
                     /translation="MNGDNQQILREIVLNPTIHGKELESIFGLSRRQLGYRIQKINLW
                     LEQEGYPKLERTSQGNFIVSSEIMTLFKRDVSEQQMLNGNNVIFSIETRRYYLMLMLF
                     SKENAMSLNHFSIDLQVSKNTVIHDINHVKEQLENHGLSLKYSRKHGYEIVGDEFEVR
                     RFFIKLIDQRLNHDITKSEVLKALNLTFEDIAYQKDKIKQVEQFLKSRFIDKSLSSLP
                     YVLCVIRRRIQSGHVMNPLNINYQYLRDTKEYQATEIMTQHEPDLPEAEKLYLTLHLL
                     STSVQWTDLQESDSISNLTMAIAQMIHHFEQITFINIEDKEKLSQQLLLHLTPAFYRI
                     KYNLTDRDELINPLQGNYQSLFHMVKQSCQSLTEYFGKSLPDNEIAYLTMLFGGSLRR
                     QDENFDGKIKAIIVCTQGTSVSQMMLYELRNLFPEIIFLDAISLRTFENYTLDYDIVF
                     SPMFVLTHKKLFITKVALSENEQRKLRKEVMKYINKESADIDKEINKLMALIERTTTV
                     NEITELRDGLEDFIANYNSISTINGSIVTQNKTLDLADLIPARHVKRMHHVENIDEAI
                     AKASDVLVANHFIDIKYIHEMQQVFDDSYMVIMQNIAIPHAYSEKHVHKTAMSMLILQ
                     EPIYMSDGTAIHIIVPIAAVDKVTHLRALLQLRDVAQDNDAIKRIIQSRKNSDVNEIL
                     KNYSNKEARENGWDSN"
     gene            12195..12662
                     /locus_tag="ACGEXS_000754"
     CDS             12195..12662
                     /locus_tag="ACGEXS_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8011790.1"
                     /translation="MGQQLVHKENIMLNLSATDKESVLSQMSDVLFQNGFVKSTFKDA
                     VIDREKEFATGLPTHLCSVAIPHTDVEHINHRTIGVAVLEKEVPFIEMGTLDQQTEVK
                     IVFMLAMDKVDDQLKLLQQLMQIFQSEEKLEQILRTKDETILATLINDYLEYN"
     gene            12685..12963
                     /locus_tag="ACGEXS_000755"
     CDS             12685..12963
                     /locus_tag="ACGEXS_000755"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8011791.1"
                     /translation="MKQVLVACGAGIATSTVVNNAIEEMAKEHNIKVDIKQIKITEVG
                     PYEDTADLLVTTAMTKKEYKFPVINARNFLTGIGIEETKQQILTELQK"
     gene            13190..14449
                     /locus_tag="ACGEXS_000756"
     CDS             13190..14449
                     /locus_tag="ACGEXS_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFF8011792.1"
                     /translation="MSYFTDFVRGFLDLGATVILPVVIFLLGLFFRQKIGAAFRSGLT
                     IGVAFVGIFLVIDLLVKNLGPAAQAMVKNLGVSLNVIDVGWPATSSIAWASSVAAFII
                     PLGIIVNVVLLVTKVTKTMNVDIWNFWHYTFTAAMVYAVSGSIWQALLAAVIFQIICL
                     KVADWTAPMMSEFFDLPGVSIATGSTISYAPGIYLVKLLQKVPGLNKLDADPETIQKR
                     FGAFGESIFVGLILGLGIGVLAGYKPGDIINLGMSMAAVMVLMPRMVKILMEGLMPVS
                     ESARTWLNKRFGEREIYIGLDAAVALGHPAVISTALILVPITVLLAVILPGNQVLPFG
                     DLATIPFVVAFIVGAARGNIIHSVIVGTIMIAISLYIATDVAPIFTDMAKGTNVQMPK
                     GSSEISSIDQGGNIVNYLIFKLFSLFN"
     gene            14467..15522
                     /locus_tag="ACGEXS_000757"
     CDS             14467..15522
                     /locus_tag="ACGEXS_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFF8011793.1"
                     /translation="MKALVKTREGHGNLELLDKEVATPLDDKVKIKVHYAGICGTDIH
                     TYEGHYKVNFPVTLGHEFSGEIVEVGADVKDFKVGDRVTSETTFYVCNECEYCKSKDY
                     NLCNHRKGIGTQVDGAFTNYVIAREESLHHIPDEVSYQSAAMTEPLACAHHGVSKIQV
                     NSGDVAVVMGPGPIGLLVVQVLKSKGATVVVTGLDNDKVRLDKAEALHMDYVVNLQQT
                     DLKTYINGITDGYGADVVVECSGAVPAARQGLDILRKKGFYSQIGIFKDAEIPFDMEK
                     VIQKEITVVGSRSQKPADWEPSLQLMADGLVNAEALVTKIYDISKWDEAYQHLKSGEG
                     IKALLKPLDLDENEGEN"
     gene            15524..15670
                     /locus_tag="ACGEXS_000758"
     CDS             15524..15670
                     /locus_tag="ACGEXS_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011794.1"
                     /translation="MVESMLTFMLGPLRQITDFYMEHLLVSNSIVIAGYFATGIFKKK
                     KVVN"
     gene            15694..16737
                     /locus_tag="ACGEXS_000759"
     CDS             15694..16737
                     /locus_tag="ACGEXS_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFF8011795.1"
                     /translation="MKALKLYGVEDLRYEDNEKPVIESANDVIIKVRATGICGSDTSR
                     YKKMGPYIKGMPFGHEFSGVVDAIGSDVTHVNVGDKVTGCPAIPCYQCEYCLKGEYAR
                     CEKLFVIGSYEPGSFAEYVKLPAQNVLKVPDNVDYIEAAMVEPSAVVAHGFYKSNIQP
                     GMTVAVMGCGSIGLLAIQWARIFGAAHIIAIDIDAHKLDIATSLGAHQTINSKEENLE
                     KFIENHYANQIDLAIESSGAKVTIGQILTLPKKGGEVVLLGIPYDDIEIDRVHFEKIL
                     RNELTVCGSWNCLSSNFPGKEWTATLHYMKTKDINVKPIISHFLPLEKGPETFDKLVN
                     KKERFDKVMFTIY"
     gene            17265..17981
                     /locus_tag="ACGEXS_000760"
     CDS             17265..17981
                     /locus_tag="ACGEXS_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFF8011796.1"
                     /translation="MIYAGILAGGIGSRMGNVPLPKQFLDIDNKPILIHTIEKFILVS
                     EFNEIIIATPAQWISHTQDILKKYNITDQRVKVVAGGTDRNETIMNIIDHIRNVNGIN
                     NDDVIVTHDAVRPFLTQRIIKENIEVAAKYGAVDTVIEAIDTIVMSKDKQNIHSIPVR
                     NEMYQGQTPQSFNIKLLQDSYRALSSEQKEILSDACKIIVESGHAVKLVRGELYNIKV
                     TTPYDLKVANAIIQGDIADD"
     gene            17974..18999
                     /locus_tag="ACGEXS_000761"
     CDS             17974..18999
                     /locus_tag="ACGEXS_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFF8011797.1"
                     /translation="MINQVYQLVAPRQFDVTYNNVDIYGNHVIVRPLYLSICAADQRY
                     YTGRRDENVLRKKLPMSLVHEAVGEVVFDSKGVFEKGTKVVMVPNTPTEQHHIIAENY
                     LASSYFRSSGYDGFMQDYVVMAHDRIVPLPNDIDLSTISYTELVSVSYHAIQRFERKS
                     IPLKTSFGIWGDGNLGYITAILLRKLYPEAKTYVFGKTDYKLSHFSFVDDIFTVNQIP
                     DDLKIDHAFECVGGKGSQVALQQIVEHISPEGSIALLGVSELPVEVNTRLVLEKGLTL
                     IGSSRSGSKDFEQVVDLYRKYPDIVEKLALLKGHEINVCTMQDIVQAFEMDLSTSWGK
                     TVLKWTI"
     gene            19021..20715
                     /gene="tarK"
                     /locus_tag="ACGEXS_000762"
     CDS             19021..20715
                     /gene="tarK"
                     /locus_tag="ACGEXS_000762"
                     /EC_number="2.7.8.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid ribitol-phosphate polymerase TarK"
                     /protein_id="HFF8011798.1"
                     /translation="MTKTKQAIHIDNIYWERVQLYIEGHSEGVDLTSGQFVLRNLTET
                     KTLEANEMKIDGNTFICRFNVAILDDGYYLPMDKYLFVYHDQLEYIGQLNPNIIDQAY
                     AALNEEQIEEYNELTTQNGKVNYLLAYDAKVFRKGGVSQHTVYTITPEIASDVNEFVF
                     DIEITLPQEKSGVIATSAHWLHKQGHKASFESRSFLFKAIFNITKLLHIKRSKTILFT
                     SDSRPNLSGNFKYVYDELLRQKVDFDYDIKTVFKENITDRRKWRDKFRLPYLLGKADY
                     IFVDDFHPLIYTVRFRPSQEIIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKA
                     YVSSETDIPFYAEAFGIREENVVPTGVPRTDVLFDEAYATQIKQEMEDELPIIKGKKV
                     ILFAPTFRGNGHGTAHYPFFKIDFERLARYCEKHNAVVLFKMHPFVKNRLNISREHRQ
                     YFIDVSDHREVNDILFVTDLLISDYSSLIYEYAVFKKPMIFYAFDLEDYITTRDFYEP
                     FESFVPGKIVQSFDALMDALDNEDYEVEKVVPFLDKHFKYQDGRSSERLVKDLFRR"
     gene            21143..>22303
                     /gene="tarF"
                     /locus_tag="ACGEXS_000763"
     CDS             21143..>22303
                     /gene="tarF"
                     /locus_tag="ACGEXS_000763"
                     /EC_number="2.7.8.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid glycerol-phosphate transferase
                     TarF"
                     /protein_id="HFF8011799.1"
                     /translation="MIKNTIKKLIEHSIYTTFKLLSKLPNKNLIYFESFHGKQYSDNP
                     KALYEYLTEHSDAQLIWGVKKGYEHIFQQHNVPYVTKFSMKWFLAMPRAKAWMINTRT
                     PDWLYKSPRTTYLQTWHGTPLKKIGLDISNVKMLGTNTQNYQDGFKKESQRWDYLVSP
                     NPYSTSIFQNAFHVSRDKILETGYPRNDKLSHKRNDTEYINGIKTRLNIPLDKKVIMY
                     APTWRDDEAIREGSYQFNVNFDIEALRQALDDDYVILLRMHYLVVTRIDEHDDFVKDV
                     SDYEDISDLYLISDALVTDYSSVMFDFGVLKRPQIFYAYDLDKYGDELRGFYMDYKKE
                     LPGPIVENQTALIDALKQIDETANEYIEARTVFYQKFCSLEDGQASQRICQTI"
BASE COUNT         7600 a         3381 c         4210 g         7112 t
ORIGIN      
        1 tattatgaaa agtgaaagta ggtaagtcta tggaaggtct taatcatcga agaaatacag
       61 aaaaagaaga gacaacacaa acgcaatcag ttgcacctaa tacaggtgaa gaggggatgt
      121 catcagcaag tacacaatca actaagacgt ccgacataca taatgaatct atcgataaac
      181 aaatggaagc taaagcgcat gaaacagcgc aaaatacaga tttaaaaaac gaagcaagaa
      241 gtttatttga taatgcaacc aaatcaatcg gtagactagc gggcaatgat gaaagcttaa
      301 atcttaattt aaaagatatg ctttctgaag tatttaagcc gcatactaaa aacgaagcag
      361 atgaaatatt tatagcgggt actgctaaaa ctacgccagc aatttgtgac atatcagaag
      421 aatgggggaa gccatggctc ttttctcgag tattcatcgc tttcacagta acatttattg
      481 gattatgggt catggcagca atttttaata acactaacgc gattccgggt ctcattttta
      541 taggggcttt aacagtacca ttatcgggtt tgttcttctt ttatgaatca aatgcgttta
      601 aaaatattag catttttgaa gttattatca tgttctttat tggcggcgta ttttcattac
      661 taagtacgat ggtattatat agatttgtcg tttttagtga tcaattcgaa aggtttggtt
      721 ctttaacatt tttcgatgca tttttagtag gattagttga agaaactgga aaagcactca
      781 ttattgttta tttcgtcaat aaattgaaaa caaataagat tttgaatgga ttattaatcg
      841 gtgctgctat tggtgcaggg ttcgcagttt ttgaatcagc aggttatatt ttgaatttcg
      901 ctttaggaga aaatgtccca ttattagata ttgtcttcac acgtgcgtgg actgcgattg
      961 gtggtcattt agtttggtca gcgattgttg gtgctgcaat agttattgcg aaagaacagc
     1021 atggctttga attcaaagat atttttgata aacgcttttt aatattcttt ttatcagccg
     1081 ttgttttaca tggcatttgg gatacatctt taactgtact tggcagtgat acgttgaaaa
     1141 tatttatttt aatcgttatt gtgtggatac ttgtattcat tttaatgggg gcaggtttaa
     1201 aacaagtgaa tttactgcag aaagaattta aagaacaaca gaaaaaagta gacgaataat
     1261 aattaaagct tatgttgctc atatgtttgt gacataagct atttttataa tttgtcttta
     1321 aaagagtgga ataggaatac tttttggagt taaaaaagtg tttcacgtta aacaaatagt
     1381 gacaattaga tttatataaa atgaacatga ttcactgaaa gtatgtaata atcattttat
     1441 tgaaattcat caaacagaaa ttaatacaat catataagca aattaaacca cgccataatc
     1501 atattggatg acttcggcgt ggtttttata gttgaagcag ggctgagaca taaatcaatg
     1561 tcccacactc ccttatcgtt caatcgttgt tcgataatcg attaaataga taccttcagg
     1621 tgttacttta taatttttaa ccttagagtt agcagcgact atttgatcgt tgtaagcaat
     1681 ataactgttt ggtacatctc gacttgataa tttaataata tcattagaaa tattgtgacg
     1741 ttccttaaca tctacagtat gattcaattg attaattaaa tcatcgacgt tgctattatt
     1801 gtagtctcct ttattaatag caccatcttt tttatatgct tgattaaaga aataacctgt
     1861 atctccacga ggaattgttc cgaaactata catcgttgca tcccatgcag aacggtcttt
     1921 taagtaacct tctatgtcat caacactttt aatgtcgatt tcaatatttg cttttttagc
     1981 atctgattgt aatacttgcg caattttcga tagctctgga cgaccgtcat acgtaattaa
     2041 cttaattttt aaagggtgtt cttttgtata accatcttta gctaataaca tttttgcttg
     2101 ttcgatattt tgtttggtta acttaggttc tttaatatat ggaattttat cattaaatgg
     2161 actcgttgca ggtttcgcat aaccttgata aatatgatct gcaatacctt gtctatcaat
     2221 gatatgatct aatgcttcac gaacggattt agtcattttt ttattagtat gattatacat
     2281 aagtaaagaa gttctaaatc cagattcttt tgacactttt aaattttgat tattttctat
     2341 gtcttgaact ttattaactg ggacatcagt tattaaatca tctttttgag attctaaatt
     2401 tctgacgcga ttattgccgt cttcttggta cgtcacagta atatgatcaa gtttcggttt
     2461 accttgccaa tagtccttaa aattcgacaa tgatattttt cgagattgct tataatcttt
     2521 tatttggtaa gggcctgtac caacaggagt ttgattaaca tctgatttag catctgtatc
     2581 ataaattgcc ataaaaggat tagctaattc agatacaagt tcagggtaag cggagttggt
     2641 tttaattgtc agtttttgac ctttagcggt aattgatgat attggtaatg aatatttgac
     2701 caagtcgctt tttttcatgc tattttcaag gctagatttc actttttctg cagtcaattt
     2761 ttgaccgttt tgaaatttaa tattatcttt taattctata tctaacgttg tatcatttgg
     2821 ttgatgatac gatttcacta atgctttttc tatttttcct tgatcatttg ttttaaataa
     2881 tgattctgca gcaccaatct taactggtac atctgtttca taaggtgcaa tagactttgt
     2941 ttttaacggt aacgaaatat ttaagtcttt gccagatgaa tgcattgagc cacatcctga
     3001 taacactaat actgctgaaa atatagttgc tagtctttta aacttcattt cattaacact
     3061 ctctttctaa ttactatgta aaacccaaca attaatattt taaaacttta ttttgttaaa
     3121 gtaaaatgtt gttcaagttt agtaattatt aaagttcaat taattgtagt aattatgctt
     3181 tttaaaaata aatattagaa atgaagttag cgacatttat agtgattcac gataaacata
     3241 tataactaag tattgagcaa ctgctgtagt actacagctt ggttatgttt agtatctttt
     3301 gctgcatata acaatagaac atgattatgc tgatttacaa tatcctttaa tttttcaaaa
     3361 gcatcttttt gcgcatcctg atcacgtaat tctttttcat atttttcttt aaaagctcca
     3421 aaaagtttag gatcatgttg gaaccattgt cgcaactcag tagaaggggc aatgtctttt
     3481 aaccaataat ctaggttagc agttcttttc gaaatacctc tcggccagac tctatcgact
     3541 aggatacgaa tagcgtcggt attatcttta ttgtcataaa tccgtccaat atctacggtc
     3601 atcttgtgaa ctcctttctt atgaaattca gtgagcatac atcaatgcat gttgtggtgg
     3661 gacgaccaaa taaattttgc gaaaatatca tctctgtcct actcccaatt aaaaaacagc
     3721 catgacaaag taaagtcata gctgttttgg tatagatgtc atttattttt acgtttagtt
     3781 aaatacttca aaccaactgc aaagaccgtg tacccggcta tggtttgtat caatgtttta
     3841 attaaattat tatttttaac aataatattt gcagtaacaa tacttacgaa atataatgca
     3901 aatactttta cgtaacgttg attaagtttc atatgagcac taaacctctt ttctcagtta
     3961 tactgcaacg cttagtcttg gaataaatgt ttcgtagtgt acgcgatcca tatcgtaatt
     4021 taaagattta agtgcttcga tcatagattg taagaatttt gtaccaccac agatataaat
     4081 ttcaggttta tttgctaaaa atacttgtaa ttcttcagca ccaatatagc cttgtttatc
     4141 ttttaagtgt gtatataatt tagcgttgtc atgatggctt gcgatactgt tgaagttgtc
     4201 tttaaaaggt aaatgttgtt cattttcagc aacttgaacc atctgtgtat ctaaaccttt
     4261 ggcagaggca gcttcataca tagctactaa aggtgtaaca ccaatacctg aacctaagaa
     4321 aagttgtggt tcagtcgtat tctctaatac gaatccacct acaggcgcag ctaaattaat
     4381 catatcgcct tctttaatct catcgtgtaa aattgttgaa acttcgcctt catgttctgt
     4441 tgtgacatca cgtttaacgc caaaagttaa atggtttttt tcacctgata cgatagaata
     4501 gtgacgttta gctctatatg gaagtttatc actagaaaca tcaactgtga tgtattggcc
     4561 tggtgtaaat tcactaaagt catattcttc agtttcaact gtaaatgatt taatgtcttc
     4621 agattcttgt ttaatattgg taattttgaa tggtttaaaa ccaatccaca tcatttgatc
     4681 ataaatttct ttttcaattt ggatgaacac atccgcaata acgccatatg cttttgccca
     4741 agcttgaatg acagggtcat tttcttctaa tcctgtcacg tcttgaatgg cttttaataa
     4801 atttttcccc acaattggat aatgttcagc ataaacttgt agtgcgcagt gtttatatgc
     4861 gactggcata atgactggtt taataacact taagttatcg atattaaccg ctgcggccat
     4921 tacagcttgt gctaatgctg aagattgcat gcctcgtttt tggttcgttt gattaaacat
     4981 gtttaaaagt tcaggatgcg ctttaaacat ttttggataa aagattgacg taatttctgt
     5041 ccctttctct ttaagtaaag gcaccgtttg tttgataatg tctttctctt gttctgtaag
     5101 catgatactc ctcctttaat ctgtatattt tgattattct actaaaaatt tcgatattca
     5161 attaattggt ttgagaaaat ataaataaaa tggcaaattt gataattgta tgacattttt
     5221 aattttttaa atacttatca ataagcattg tgtacaattg tctgtttgca caccgacgat
     5281 tgagcgcatt tatttgacta attcaaaaaa cattgttgtt ttcctagaaa aaagtaaaca
     5341 tgataataaa aatgtgaaag tgtaaataat cactggcgaa gtacgaagac taaagacatc
     5401 taagatgtaa tcgtatacaa attaaaaagg tgtaaaaatt aaaataaaat gtgaaataaa
     5461 tcacaattta atattgaccc agtacttaat gcatgttaca ttttatatgt gaaataaatc
     5521 acaaacttaa aagcggatga cacatgacct tttaagttat gcgttgaaaa taaaagagat
     5581 gtttatttgc ttttgtatcg tcaataagca gcattaaact aacatatttg aagctacatg
     5641 tatgttagtg aattaatcat aagggagttt ttgtaatgaa caaatttaaa gggaacaaag
     5701 ttgtattaat aggtaatggt gcagtaggtt caagctacgc attttcatta gtgaaccaaa
     5761 gcattgttga tgaattagtc atcattgatt tagacactga aaaagttcga ggagatgtta
     5821 tggatttaaa acatgccaca ccatattctc caacaacagt tcgtgtgaaa gctggcgaat
     5881 acagtgattg tcatgatgcg gatctagttg tcatctgtgc tggtgctgca caaaaacctg
     5941 gagaaacacg tttagattta gtatctaaaa acttgaaaat attcaaatca attgttggtg
     6001 aagtaatggc atcaaaattt gatggtattt tcttggtagc tacaaatcct gttgatattt
     6061 tagcgtatgc aacatggaaa ttctctggtt tacctaaaga acgtgttata ggttctggta
     6121 caattttaga ctctgcacgc tttagattat tgttaagcga agcgttcgat gttgcgccac
     6181 gtagcgtcga tgctcaaatt attggtgaac atggtgacac tgaattacca gtatggtcac
     6241 acgctaatat tgcgggtcaa cctttgaaga cattacttga acaacgtcct gagggcaaag
     6301 cgcaaattga acaaattttt gttcaaacac gtgatgcagc atatgacatt attcaagcta
     6361 aaggtgccac ttattatggt gttgcaatgg gattagctag aattactgaa gcgattttca
     6421 gaaatgaaga tgccgtattg actgtatcag cattattaga aggcgaatat gaggaagaag
     6481 atgtttatat tggtgttcca gcagtcatca atagaaacgg tattcgcaac gtcgtagaaa
     6541 tcccattaaa cgacgaagaa caaagcaagt tcgcacattc agctaaaaca ttaaaagata
     6601 ttatggctga agcagaagaa cttaaataac tttttataaa atctatacca tcccaaaaat
     6661 tgtaaaacct tacccaaaaa attgtataaa gggctatttg atacaactat atgtgtcgag
     6721 tagccctatt tttaatgtag tgaaagtcgt tgttgaaatt aaattcaaat ctggcactta
     6781 agctttaatc atagcattaa gatgattgtc tagcagaggc gatttgcggg ctcactacag
     6841 tgcatgatga acttaatgct tcaaatgtaa cattaaaaat aaaagcaacg atgtcacttc
     6901 ttacttcgta catcgttgcc attagttctg ttgatatttc ggttagtctt aatccccgag
     6961 caattcttca atttcatttt tgataactgt aacgtgaggc ccataaatta cttgcacacc
     7021 agtgccttgc tggattacac ctttggcacc agtactttcg agtaatactt tatcgacttt
     7081 gtcattttga tgaagtgtga cgcgtagtct cgttgcacaa cagtcaacga tttcaatgtt
     7141 atctttgcct cccaaaccag caacaatagt ttgtgctctt tcagtagcct caacttgttg
     7201 tgctgcagct ttatcttctc gaccaggtgt tttgaaatta aatttcgtaa ttaagaatct
     7261 gaaaacgatg taatacaaac agaaccacac aattccaata ggtatgacgt ataggtagtt
     7321 tgttttacta ttaccttgta gcacaccaaa gagtaagaaa tcgataaagc ctccactgaa
     7381 ggtttgacca attgtaatgt tgaaaatgtc tgccatcata aatgctaatc catcaaagaa
     7441 ggcatggatt acataaagaa taggtgcgac aaacaagaaa ctaaactcta aaggttcggt
     7501 aatacctgtt aaaaatgaag tgagtgcagc ggataacatt aaaccgccga caactttttt
     7561 atgttcaggt ttagctgtgt gataaattgc aagtgcggca ccacataagc cgaacatcat
     7621 cgtaataaaa cggcctgaca taaagcgtga cacacctgaa taatacttcg tcacatctgg
     7681 atcaccaagt tgagcaaaga agatgttctg cgtaccttga actaagtgcc ctttgacttc
     7741 taaagtacca ccaagtgccg tctgccaaaa cggtaagtaa aaaatatggt gtaaaccgag
     7801 tggacctaac aatcttaaga tgaagccata aacaaaagta ccgatggcac ctgttttcgt
     7861 tacaaatcca ccaacatgat aaatgccggc ttgtatgctt ggccaaatga aaaacatcaa
     7921 tacacctaaa aagattgcgg caaatgctgt gacaataggg acaaatctag agccaccaaa
     7981 gaaacctaaa tacggtggta ataccacttt gtgatatttg ttgtgaagta ttgcggtcat
     8041 aatacctgtg ataatcccgc caaaaacacc ggtttcaacc gtttgtatac cgagcaccat
     8101 gccttgtcca ttttgtgcaa gctgatcttt tgccaatgtg cccgtgatag ttaataagcc
     8161 attcatagtt gcgttcataa ttaagaaacc gagcagcgca gctaaacctg cagtaccttt
     8221 atcgcttcta gataatccga ttgcgacacc aattgcaaag atgaccggta aattttggaa
     8281 aacaatacta cctgcagctg acattaatgt aaaaatattt tgtaataagg taatatctaa
     8341 aatagggtat gctttaacgg tgtttggatt acttaatgca ccaccgatac ccaacaatag
     8401 acctgcagct ggtaagattg cgataggtaa cataaaggac ttgccgaact gctgtgcttt
     8461 ttcaaataaa gatttcatca acatccctcc taattattct caatatagct tttgagaaat
     8521 ttaatatcaa tatatattct gtgtatgaaa atatttttca taaaaattgt ttgaatcatg
     8581 taacaatcat ataaattgct gtttatattg ttgtgaaaat gagttgacaa aagtcggtat
     8641 agatatgtag acatcctatt tttagcgagg taggttgata aggcatatcg gataaattta
     8701 taagccataa aatagataaa tagtgtttgt atccaaaaat atgagaaagt taaaactatt
     8761 tttcaaaata aacatgattc accacataaa taaatataca taagctatcg ataacaactt
     8821 aagaaaggtg gatatataaa tgaaaagaaa gattattatg gattgtgatc caggacacga
     8881 tgatgcaata gcattaattt tagcgggggc aattgacagt ccactagaga tattagctgt
     8941 aacaacagtc gcaggtaatc aatcagttga caagaatacg acaaacgcct tgaacgtatt
     9001 ggatattatg ggacgccaag atatagcagt agcgaaaggt gcggataggc cgttaattaa
     9061 accagctgcc tttgcttctg aaatacatgg ggaatctgga ttagatggtc cgaaactacc
     9121 gtcgacacca tcacgtcaag cagttgcaat gccagcatca gatgtgatta taaacaaagt
     9181 gatgacgagt gatacacctg taacaattgt agcgacaggt cctcttacga atgtagcaac
     9241 ggcattgatt cgtgagccaa gaatcgctga gcatattgaa tctattactt tgatgggtgg
     9301 tggtacattt ggaaattgga cgcctacagc agaattcaat atttgggtag atgctgaagc
     9361 agcgaagcgt gtttttgaaa gtgggattac tataaatgtg tttggtttag atgtaacaca
     9421 tcaagtttta gccgacgatc acgtgattga acgctttgaa agtatcaata atcctgttgc
     9481 acagttcgtc gtagaattat tgcaattctt taagaagaca tacaagactc actttaatat
     9541 ggatggtggt ccaatacatg atgcttgtac aattttgtat ttgttacaac cagaattgtt
     9601 tacaatggta cccgttaata tcgacattga acatcaaagt ccactaactt atggcactat
     9661 ggctgtcgat ttaaatcatg ttacaggtaa gcctgccaat gcttattttg ctacagcagt
     9721 tgatgttgaa gaagtgtgga acttgataga ccataagtta cgtacatacg aataataata
     9781 cttaattaaa tagatacagt taaccctaag gcgcctgata taagcgtcct tagggttttt
     9841 gtattgggtt acaatggtca tgacaatttg ataatgattg agatatgtat gttaaaaatg
     9901 cttcaaaaaa acacaaaaca caaaaatata tgcaaaaatt tcaattgctt tattttaaaa
     9961 gattaaaatc acaaaaaata tagatataat tgaaatgttg ttaaaacatc acaatgatgt
    10021 acatgctcat tttgaaaata tgtgttcaaa gtgtgtacat cggtgtgacg attgatgata
    10081 aattgagctc agatgctcaa aggagaatcg aacatgaacg gggataatca gcaaatactc
    10141 agagaaattg tattgaatcc tactattcat ggtaaagaac ttgaatcgat atttggtttg
    10201 tctcgtagac aactaggata tcgcattcaa aaaatcaatt tgtggcttga acaagagggt
    10261 tatccaaaac ttgaaagaac aagccaagga aattttattg taagttctga aatcatgacg
    10321 ttattcaagc gagatgtatc ggagcagcaa atgttaaacg gcaacaatgt catttttagc
    10381 atagaaacac gtcgttatta tttaatgctc atgcttttta gtaaggaaaa cgcaatgtct
    10441 ctaaaccatt tttcaattga tttacaagtc agtaaaaata ctgtcattca cgatataaat
    10501 catgtgaaag agcaattgga aaatcatggt ttgtcactta agtattctcg aaaacatggt
    10561 tatgaaattg ttggtgatga atttgaagtt cgccgtttct tcattaagtt gattgatcaa
    10621 aggttgaatc atgatattac taaaagtgaa gttttaaagg cgctcaactt aacattcgaa
    10681 gatatcgcat atcaaaaaga caagatcaaa caggtagaac aatttttgaa gagtcgcttt
    10741 atagacaaat cacttagttc attgccttat gtcctttgtg tgattcgtag acgaattcaa
    10801 agtggtcatg tgatgaatcc attaaatatt aattatcagt atttgaggga tacgaaagaa
    10861 tatcaagcaa cggagattat gacgcaacat gagccggatt tgccagaagc ggaaaagtta
    10921 tatttgacat tacacttact ttcaacaagt gtgcaatgga ctgatttgca agaatcagat
    10981 agcatatcga atttaacgat ggccatcgct caaatgattc accattttga acaaatcact
    11041 tttattaaca ttgaagataa ggagaaatta tcacagcaac tcttgttaca tttaacgcct
    11101 gctttttata ggattaaata taacttaacg gatcgtgatg aattaataaa tcctttacaa
    11161 ggaaattatc aatccttatt tcatatggtg aaacaatcat gtcaatcgtt aactgaatat
    11221 ttcggaaaat cgttgcctga taatgaaata gcatatttaa ccatgttgtt cggaggtagt
    11281 ttgagacgtc aagatgaaaa cttcgatggc aagataaaag ctattatcgt gtgtacacaa
    11341 ggcacgtcag tatcacaaat gatgttatac gagttgcgaa acttatttcc agaaattatt
    11401 ttcttagatg cgatttcact tagaacattt gaaaattaca cattagatta tgacatcgtc
    11461 ttttcaccaa tgtttgtcct aacacataaa aaattattta tcacaaaagt agctttatct
    11521 gaaaatgagc aacgaaagtt acgtaaagaa gtgatgaagt acattaataa ggaatcggct
    11581 gacattgata aggaaataaa caagttaatg gcattaattg aacgcacaac gacagttaat
    11641 gaaattacag aactacgtga tggtttagaa gattttattg ccaattataa ttcaatttca
    11701 accattaatg gatcgattgt cacacaaaat aagacattag atttagctga cttgataccg
    11761 gcaaggcacg tgaaaagaat gcatcatgtt gaaaatattg atgaagctat tgctaaagca
    11821 agtgatgtgt tagttgctaa tcattttatt gatattaaat atattcatga gatgcaacag
    11881 gtatttgatg attcgtatat ggttatcatg caaaatattg ctattccaca tgcatactct
    11941 gaaaagcatg tacataaaac agcgatgagt atgttgatat tacaagaacc aatatacatg
    12001 tcagatggca cagcaatcca tattattgta cctattgctg ctgttgataa agtgacacac
    12061 ttaagagcgt tactacaatt gagagatgtg gcgcaagaca atgacgcaat taagcgcatc
    12121 atacaaagtc gcaaaaattc tgatgtaaat gagattttaa aaaattattc aaataaagaa
    12181 gcgagggaaa atggatggga cagcaattag tgcataaaga aaatataatg ctcaatttgt
    12241 cggcaactga taaagaatcc gtattgtcac aaatgtcaga tgtgttattt caaaatgggt
    12301 tcgtgaagtc aacgtttaaa gatgcagtca tcgacagaga aaaagaattt gctactggtt
    12361 taccaacgca tctatgttcg gtcgctatac cgcatacaga tgtcgaacat attaaccata
    12421 gaacgatagg tgtggctgtt ctagaaaaag aagtgccgtt tattgaaatg ggaacacttg
    12481 atcaacagac agaagtgaaa atcgttttta tgttagcaat ggataaagta gatgatcaac
    12541 ttaagctgtt acaacagttg atgcaaattt ttcaaagtga agaaaaattg gagcagattc
    12601 tacgaacgaa agatgaaaca attttagcaa cactaatcaa tgattatttg gaatataact
    12661 aaaaattaat tggaggaatt gaaaatgaaa caagtattag tagcgtgtgg tgcaggtatt
    12721 gcaacgtcaa cagtagtaaa taatgcaatt gaagaaatgg caaaggaaca caatattaaa
    12781 gtagatatta aacaaatcaa aattacagaa gttggacctt atgaagacac tgcagattta
    12841 ttagttacaa ctgcaatgac aaaaaaagaa tataaattcc cagttatcaa cgcacgtaat
    12901 ttcttaactg gtattggtat tgaagaaaca aaacaacaaa tcttaacaga gttacaaaaa
    12961 taacggaatt gatatgtaac gtgggtcgat aacatattaa tatatgcaaa ttgcatataa
    13021 atgactatca cgacaaatga ttgttgatga catagaaacg cagtgactgt aattcaaaag
    13081 gactgcagtc gaatttagca agggttgtcg ttgaaatgac tgtaacgtca aatgtaatca
    13141 caatcggaaa gtatcgtgac aatgcatata taacagggag ggtttaaata tgagttactt
    13201 cactgatttt gtaaggggat ttttagattt aggtgcaact gttattttac cggttgtcat
    13261 attcttgctt ggcctattct ttaggcagaa aattggagcg gcatttaggt ctggtttaac
    13321 aataggtgtg gcttttgtag ggattttctt agtcatcgat ttattagtta aaaatttagg
    13381 gccagcagca caagcgatgg ttaaaaattt aggcgtcagt ctgaatgtga ttgatgtagg
    13441 ttggccagca acatcatcta tcgcttgggc atcatctgtc gcagcattta ttattccact
    13501 cggaatcata gttaacgttg tattgctagt aactaaagtg acaaagacga tgaatgtaga
    13561 tatttggaat ttttggcatt atacgtttac agcagcaatg gtttatgccg tatcaggcag
    13621 tatttggcaa gcgttattag cagcagttat tttccaaatt atctgtttga aagtagcaga
    13681 ttggacagca ccgatgatga gtgagttctt tgatttacca ggtgtatcga ttgctacagg
    13741 aagtacaatt tcttatgcac caggtattta cttagttaaa ttgttacaaa aagtacccgg
    13801 tctgaataag ttagatgctg atcctgaaac aattcaaaaa cgttttggcg catttggaga
    13861 gtctatattt gtcggcttaa ttttaggttt aggtattggt gtgttagcag gttacaaacc
    13921 tggagacatc attaatttag gaatgtcaat ggctgcagta atggtattaa tgcctagaat
    13981 ggtaaaaatc ttaatggaag gtttaatgcc agtttcagag tctgcaagaa catggctaaa
    14041 taaacgtttt ggcgaacgtg aaatttatat tggattggat gcggctgtag cattaggtca
    14101 tccagcagtt atttcgacag cattaatttt agtacctatc actgttttat tagccgttat
    14161 tttaccagga aaccaagtac taccttttgg tgacttagca acgataccat ttgttgtcgc
    14221 gtttattgtt ggtgcagcaa gaggaaacat tattcattct gtcattgtgg gtacgattat
    14281 gattgcaatt tcactatata ttgcaacaga cgtagcaccc attttcacag atatggcgaa
    14341 aggtacgaat gtacaaatgc caaaaggttc atctgaaatt tcaagtattg atcaaggtgg
    14401 taatatcgtt aactatctta tctttaaact atttagtcta ttcaattaaa aatcgaggtg
    14461 tttgtggtga aagctttagt aaaaacaaga gaaggacatg gcaacttaga acttcttgat
    14521 aaagaagttg caacaccgct agatgataaa gtaaagatta aagtacatta tgcaggaatt
    14581 tgtggcacag atattcatac ttatgaaggt cattataaag ttaattttcc agtgacatta
    14641 ggtcatgaat tttctggcga aatcgttgaa gttggagcag acgttaaaga ttttaaagtt
    14701 ggtgaccgtg tcacatctga aacgacattc tatgtttgta atgagtgtga atactgtaaa
    14761 tcaaaagact ataatttatg caaccatcga aaaggtattg gaacacaagt tgatggcgca
    14821 tttactaatt atgtcattgc acgtgaagaa agtttgcatc atattccaga cgaagtatcg
    14881 tatcagtctg cagctatgac agaaccatta gcatgtgcac atcatggcgt ttctaagatt
    14941 caagtcaatt caggcgatgt agcagttgta atgggacctg ggccaatcgg attacttgta
    15001 gtacaagtgt taaaaagtaa aggcgcaact gttgtggtaa ctggattgga caatgacaaa
    15061 gtcagattag ataaagcaga agcattgcac atggattatg tagtcaattt acaacaaaca
    15121 gacttaaaaa cgtatatcaa tggaattaca gacggttacg gtgcagatgt tgttgttgaa
    15181 tgttcaggtg cagttccagc agcacgacaa ggtttggata ttttacgcaa aaaaggtttc
    15241 tacagtcaaa taggtatttt taaggatgct gaaattccat ttgatatgga aaaagtgatt
    15301 caaaaagaaa taacagttgt tggtagtaga agtcaaaagc cagcagattg ggaaccttca
    15361 ttgcaactta tggcggatgg tttagtaaat gctgaagctt tggtgacaaa aatatatgat
    15421 atttcgaaat gggacgaggc gtatcaacat ttaaaatccg gcgaaggtat taaagcatta
    15481 cttaagccgc tcgatttaga tgaaaatgaa ggagagaatt aatatggtag aatcaatgct
    15541 aacttttatg cttgggccat taagacaaat cactgatttt tatatggaac atttactcgt
    15601 aagtaattcc attgtcattg caggttattt tgcgacaggt atttttaaaa agaaaaaagt
    15661 tgtgaattaa atcaaatttg aggtgattta caagtgaaag cattgaaatt atatggcgtg
    15721 gaagatttac ggtatgagga taatgaaaag ccagtcattg aaagtgcgaa tgacgttatt
    15781 attaaagtac gagcgactgg catatgtggt tcagacacgt cacgatacaa aaaaatgggg
    15841 ccatacatta aaggtatgcc atttggtcat gaattttcag gtgtagtaga tgccattgga
    15901 agtgatgtta cgcatgttaa tgtgggcgac aaagtgacag gttgcccagc aataccttgt
    15961 tatcaatgcg agtattgttt aaaaggtgaa tatgcacgat gtgaaaagtt attcgtcatt
    16021 ggctcatatg aacctggatc gttcgcggaa tatgtcaaat tgccagcgca aaatgtttta
    16081 aaggttccag acaatgttga ttacattgaa gcagcaatgg ttgagccatc agccgttgtt
    16141 gcgcatgggt tttataaatc gaatatacaa cctggtatga ctgttgcagt aatggggtgt
    16201 ggcagtatag gtttgttagc tattcaatgg gcacgaatat ttggtgctgc acatatcatc
    16261 gctatagata tagatgcgca taaactagat attgcaacat cattgggcgc acatcaaaca
    16321 atcaattcaa aagaagaaaa tcttgagaaa ttcatcgaaa atcattacgc caatcaaatc
    16381 gatttagcta tagaatcatc aggtgctaaa gttacgattg gtcaaatatt gacgctacct
    16441 aaaaaaggtg gcgaggtggt attactcgga ataccatatg atgatattga gattgatcgc
    16501 gttcattttg aaaaaattct gcgtaacgag ttgacagtat gtggctcttg gaactgtttg
    16561 tccagtaatt ttccgggcaa agagtggacg gcaaccttac attatatgaa gacgaaagat
    16621 attaatgtaa agcctattat ttctcatttt ttaccgttag aaaaaggccc ggagacattt
    16681 gataaattag ttaacaagaa agaacgattt gataaagtca tgtttacgat ttattagtat
    16741 gcacctttga ggacgaaaac gctggtatag ttatagctat gaaagtgcga atgccgtctg
    16801 gtctatagat actatcgaaa taattcatct tcgaatatac gttgataaat agccggttta
    16861 cttgtgtgaa atatgcttgt gaatcggttg ttttgcattt tgtatactta aaatgagatg
    16921 gcaatatttg ataattttta aagtgaaaat caagtacagc cacttaataa gataaattta
    16981 ttataatata tggtaaaatg atggcagtaa taatgaattt gaaaaagagt aaacattaat
    17041 acctttaaca atttaatatc gtcagagtta atgattaact gcatggcaaa acaacttaga
    17101 atggtcagtt acaaaaatac atttttataa aaaattatca cactattgtg acaactatct
    17161 ttggattaat aaaagaggca agtgagcaat aggttaggct tatgtgcggg cataggtcag
    17221 taatgtataa atggaaatga tgtaatgaca gaatggagga caacatgatt tatgcaggta
    17281 ttttagcagg aggtattggt tcgagaatgg ggaacgtgcc attaccaaaa caatttttag
    17341 atattgataa taaaccgatt ttaatccata caattgagaa gttcatttta gtgagtgaat
    17401 ttaatgagat tattatcgca acgccagcac agtggatttc ccatacacag gatattttaa
    17461 aaaaatataa cattacagat caacgtgtca aagtagttgc aggtggtacg gatcgaaatg
    17521 aaacaattat gaacattatc gaccatattc gcaatgtaaa tggaattaat aatgatgatg
    17581 tgattgtaac tcatgatgcc gtaagaccat ttttaactca acgtattatt aaagagaaca
    17641 ttgaagtagc agcaaaatat ggtgcagtag atacagtcat tgaagcaatt gatacgattg
    17701 taatgtctaa agataaacag aacatacaca gtatccctgt aaggaatgaa atgtatcaag
    17761 gccaaacacc acaatcattt aatattaaat tattacaaga tagttatcgc gccttaagta
    17821 gtgaacaaaa agaaatctta tcagatgcat gtaaaatcat tgtcgaatct ggacatgcag
    17881 ttaaattggt acgtggagaa ctatacaaca ttaaagtgac aacaccgtat gatttaaaag
    17941 tagcaaatgc cattattcaa ggtgatattg ccgatgatta atcaagtata tcaactcgtt
    18001 gcaccgagac agttcgacgt cacatataat aatgttgata tttatggtaa tcatgtcatc
    18061 gtaagacctt tatacttgtc tatttgtgca gctgatcaaa ggtattacac aggtcgaaga
    18121 gatgaaaatg tactacgcaa aaaattacca atgtcattag ttcatgaagc tgttggtgaa
    18181 gttgtatttg atagtaaagg ggtatttgaa aaaggtacga aagtagtaat ggtaccgaat
    18241 acacctacag agcaacatca tattattgcg gagaattact tagcctctag ttattttaga
    18301 tctagtggtt atgatggttt tatgcaagac tacgttgtga tggcacatga tcgtatcgtt
    18361 ccgctgccta atgacattga tttgagtacg atttcataca cagagttagt gtcagtaagt
    18421 tatcatgcta tacaacgatt tgaacgtaaa tctatacctt tgaaaaccag ctttggtatt
    18481 tggggtgatg gtaacttagg ttatattact gctattttgc tacgtaagtt gtacccagaa
    18541 gctaaaactt atgtatttgg taagacagac tataaattaa gtcatttttc atttgtagat
    18601 gacatcttta cagtaaatca aataccagat gatcttaaaa ttgatcatgc atttgaatgt
    18661 gttggaggta aaggaagtca agttgcactt caacaaatag ttgaacatat ttcaccagaa
    18721 ggcagtattg ctttgttagg cgtaagtgaa ttacccgtgg aagtgaatac acgattagta
    18781 cttgaaaaag gattaacgtt gattggtagt agtcgaagcg gctctaaaga ttttgagcaa
    18841 gttgttgatt tatatcgtaa gtacccagac atagttgaaa agttagcatt attaaaagga
    18901 catgaaatta atgtatgtac gatgcaagat atcgtccaag cgtttgaaat ggatttatcg
    18961 acatcttggg gaaaaacagt attgaaatgg acgatttaat aaaccgatgg gggaataaca
    19021 atgacaaaaa cgaaacaagc aatacatatt gataacatat actgggaacg tgttcagtta
    19081 tatattgaag gacatagtga aggtgtcgat ttaacatcag gacaatttgt tctgaggaat
    19141 ttaaccgaaa caaaaacatt agaagcaaat gaaatgaaaa tagacggtaa tacatttata
    19201 tgtagattca acgtcgcaat attagacgat gggtattatt taccaatgga taaatattta
    19261 tttgtttatc atgaccagtt agagtatatt ggacaactta atccaaatat tattgatcaa
    19321 gcttatgcgg cattaaatga agagcaaatt gaagaataca atgagctgac tacacaaaat
    19381 ggaaaagtga actatttatt agcgtatgat gctaaagttt tccgtaaagg tggcgtatca
    19441 caacatacgg tctataccat tactccggaa atagcaagtg acgttaacga atttgtattt
    19501 gatattgaaa tcaccttacc tcaagagaaa tcaggggtca ttgcgacaag tgcacactgg
    19561 cttcataaac aaggtcataa agcttcattt gaaagtagaa gtttcttatt taaagctatt
    19621 tttaatatta caaagttact acatattaaa agaagcaaaa caatattatt cacatcagat
    19681 tcgcgtccga atttatcagg gaatttcaag tatgtatatg atgagttact acgccaaaaa
    19741 gtagattttg attatgatat taaaacggta tttaaggaga atattacgga tagacgcaaa
    19801 tggagagaca agtttagatt gccatattta cttggtaagg ccgattatat ttttgttgat
    19861 gatttccatc cattaattta tacggttcgc tttagaccat cacaagaaat tattcaagtg
    19921 tggcatgctg ttggtgcctt taaaacagtt ggctttagtc gtacaggtaa aaaaggtggt
    19981 ccgtttatcg attcattaaa ccatcgtagt tacacgaaag catatgtttc atcagaaacc
    20041 gatattccat tttatgctga agcatttgga attagagaag aaaatgttgt accaacaggt
    20101 gtaccacgta ctgatgtact atttgatgaa gcttatgcaa cacaaattaa acaagagatg
    20161 gaagatgaat tgccaattat aaaaggtaag aaagttattc tattcgcacc gacatttaga
    20221 ggtaatggtc acggtacggc acattatcca ttttttaaaa ttgattttga acgtttagca
    20281 agatactgcg agaagcataa tgcagttgtg ttattcaaaa tgcatccgtt cgtaaaaaat
    20341 agacttaata tttcacgtga acatagacaa tactttatcg atgtgtcaga tcatcgtgaa
    20401 gttaacgata ttctctttgt tacagacttg ttgattagtg attattcatc tttaatatat
    20461 gaatatgcag tatttaaaaa gccgatgatt ttctatgcat ttgacttaga agattacatt
    20521 acgacgcgtg atttctatga accatttgaa tcatttgttc caggtaaaat tgtacagtcc
    20581 tttgatgcat taatggatgc tttggacaat gaagattatg aggttgaaaa agttgtgcca
    20641 ttcttagata aacattttaa atatcaagat ggtcgctcaa gtgaacgttt agtcaaagat
    20701 ttgtttagac gctaatgttg gcatatatta cttgccatgc aaatgagtca taaggcatag
    20761 ttttcaagaa gggttctgac aatgaagtga gcaacatgtc atgatgagaa gcaggactat
    20821 acaatgagaa taaccttttg atttttcatg cataaggcga ttcaatcaaa agcaatcacc
    20881 ttccaactga attgtcattt tgtaaaataa aataatcgat ccaatcgtta tctaattcat
    20941 aaatgtgtaa acacatacgt tatgaatgga taacgatttt ttgttatgtt aaagtggtac
    21001 attaatcatg tatttcgtat gataattaac gacaagtgta atggttaaat gtattttatg
    21061 atgaaatgct ataataggca tggttacaat gagcttgctc atacatatta atataattac
    21121 aaaaacacgt cggaggtacg acatgattaa aaatacaatt aaaaaattga tagaacatag
    21181 tatatatacg acttttaaat tactatcaaa attgccaaac aagaatctaa tttattttga
    21241 aagctttcat ggtaaacaat acagcgacaa ccccaaagca ttatatgaat acttaactga
    21301 acatagcgat gcccaattaa tatggggtgt gaaaaaagga tatgaacaca tattccaaca
    21361 gcacaatgta ccatatgtta caaagttttc aatgaaatgg tttttagcga tgccaagagc
    21421 gaaagcgtgg atgattaaca cacgtacacc agattggtta tataaatcac cgcgaacgac
    21481 gtacttacaa acatggcatg gcacgccatt aaaaaagatt ggtttggata ttagtaacgt
    21541 taaaatgcta ggaacaaata ctcaaaatta ccaagatggc tttaaaaaag aaagccaacg
    21601 gtgggattat ctagtgtcac ctaatccata ttcgacatcg atatttcaaa atgcatttca
    21661 tgttagtcga gataagattt tggaaacagg ttatccaaga aatgataaat tatcacataa
    21721 acgcaatgat actgaatata ttaatggtat taagacaaga ttaaatattc cattagataa
    21781 aaaagtgatt atgtacgcgc caacttggcg tgacgatgaa gcgattcgag aaggttcata
    21841 tcaatttaat gttaactttg atatagaagc tttgcgtcaa gcgctggatg atgattatgt
    21901 tattttatta cgcatgcatt atttagttgt gacacgtatt gatgaacatg atgattttgt
    21961 gaaagacgtt tcagattatg aagacatttc ggatttatac ttaatcagcg atgcgttagt
    22021 taccgactac tcatctgtca tgttcgactt cggtgtatta aagcgtccgc aaattttcta
    22081 tgcatatgac ttagataaat atggcgatga gcttagaggt ttttacatgg attataaaaa
    22141 agagttgcca ggtccaattg ttgaaaatca aacagcactc attgatgcat taaaacaaat
    22201 cgatgagact gcaaatgagt atattgaagc acgaacggta ttttatcaaa aattctgttc
    22261 attagaagat ggacaagcgt cacaacgaat ttgccaaacg att
//
LOCUS       DAXWFK010000025        21762 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.025, whole genome shotgun sequence.
ACCESSION   DAXWFK010000025 DAXWFK010000000
VERSION     DAXWFK010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 21762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21762
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.025"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..208
                     /locus_tag="ACGEXS_000764"
     CDS             <1..208
                     /locus_tag="ACGEXS_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFF8011800.1"
                     /translation="FNYVFNDIGEDERQHSIIVGDSLTSDILGGINAGIATCWFNFRG
                     FDHNPGIIPDYEINSWKQLNDIVR"
     gene            complement(317..757)
                     /locus_tag="ACGEXS_000765"
     CDS             complement(317..757)
                     /locus_tag="ACGEXS_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFF8011801.1"
                     /translation="MSEIKRLEINYKTDELFENFRAFGNKDLYMVNELNGQMIDASSD
                     SPFYGIFVGDQLGARMALLKKGDVEEIYFPDFEDYILLWKLEVLPKYQNRGYASELID
                     FAKSFNMPIKAIGRNDSKDFFLHHGFTDVEAKNIEGHDVLLWKP"
     gene            952..2565
                     /gene="bshC"
                     /locus_tag="ACGEXS_000766"
     CDS             952..2565
                     /gene="bshC"
                     /locus_tag="ACGEXS_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HFF8011802.1"
                     /translation="MDCKVVSLNEKDQFIPKIKSSDPVITGLFQYDAAQQTSFEKRMS
                     KENNGREAALANVIREYMSDLKLSSEQELNIQHLANGSKVVIGGQQAGLFGGPLYTFH
                     KIFSIITLSKELTDTHKQQVVPVFWIAGEDHDFDEVNHTFVYNENHGSLHKVKYHTME
                     MPETTVSRYYPDKAELKQTLKTMFIHMKETVHTQGLLEICDRIIDQYDSWTDMFKALL
                     HETFKAYGVLFIDAQFEPLRKMEAPMFKKILKKHQLLDDAFRATQQRTQNQGLNAMIQ
                     TDTNVHLFLHDENMRQLVSYDGKHFKLNKTDKTYIKEEIINIAENQPELFSNNVVTRP
                     LMEEWLFNTVAFVGGPSEIKYWAELKDVFELFDVEMPIVMPRLRITYLNDRIEKLLSK
                     YNIPLEKVLVDGVEGERSKFIREQASHQFIEKVEGMIEQQRRLNKDLLDEVAGNQNNI
                     NLVNKNNEIHIQQYDYLLKRYLLNIERENDISMKQFREIQETLHPMGGLQERIWNPLQ
                     ILNDFGTDVFKPSTYPPLSYTFDHIIIKP"
     gene            2709..3140
                     /gene="mraZ"
                     /locus_tag="ACGEXS_000767"
     CDS             2709..3140
                     /gene="mraZ"
                     /locus_tag="ACGEXS_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFF8011803.1"
                     /translation="MFMGEYDHQLDTKGRMIIPSKFRYDLNERFIITRGLDKCLFGYT
                     LDEWQQIEEKMKTLPMTKKDARKFMRMFFSGAVEVELDKQGRINIPQNLRKYANLTKE
                     CTVIGVSNRIEIWDRETWNDFYEESEESFEDIAEDLIDFDF"
     gene            3156..4091
                     /gene="rsmH"
                     /locus_tag="ACGEXS_000768"
     CDS             3156..4091
                     /gene="rsmH"
                     /locus_tag="ACGEXS_000768"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017724209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFF8011804.1"
                     /translation="MFHHISVMLNETIDYLNVKENGVYIDCTLGGAGHALYLLNQLND
                     DGRLIAIDQDQTAIDNAKEVLKDHLHKVTFVHSNFRELTQILKDLNIEKVDGIYYDLG
                     VSSPQLDIPERGFSYHHDATLDMRMDQTQELTAYEIVNNWSYEALVKIFYRYGEEKFS
                     KQIARRIEAHREQQPITTTLELVDIIKEGIPAKARRKGGHPAKRVFQALRIAVNDELS
                     AFEDSIEQAIELVKVDGRISVITFHSLEDRLCKQVFQEYEKGPEVPRGLPVIPEAYTP
                     KLKRVNRKPITATEEDLDDNNRARSAKLRVAEILK"
     gene            4105..4506
                     /gene="ftsL"
                     /locus_tag="ACGEXS_000769"
     CDS             4105..4506
                     /gene="ftsL"
                     /locus_tag="ACGEXS_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFF8011805.1"
                     /translation="MAVEKVYQPYDEQVYNSIPKQQPQTKPEKKSVSRKVVVQLTKFE
                     KVLYITLITVIAMLSIYMLSLKMDAYDTRGKIADLDYKIDKQSSENSALQSEIKKNSS
                     YERIYEKAKKQGMSLENDNVKVVRSNGEAKN"
     gene            4487..6721
                     /locus_tag="ACGEXS_000770"
     CDS             4487..6721
                     /locus_tag="ACGEXS_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HFF8011806.1"
                     /translation="MAKQKIKIKKNKIGAVLLVGLFGLLFFILVLRISYIMITGHSNG
                     QDLVMKANEKYLVKNAQQPERGKIYDRNGKVLAEDVERYKLVAVIDKKASANSKKPRH
                     VVDKKETAKKLSTVINMKPEEIEKRLSQKKAFQIEFGRKGTNLTYQDKLKIEKMNLPG
                     ISLLPETERFYPNGNFASHLIGRAQKNPDTGELKGALGVEKIFDSYLSGSKGSLRYIH
                     DIWGYIAPNTKKEKQPKRGDDVHLTIDSNIQVFVEEALDGMVERYQPKDLFAVVMDAK
                     TGEILAYSQRPTFNPETGKDFGKKWANDLYQNTYEPGSTFKSYGLAAAIQEGAFDPDK
                     KYKSGHRDIMGSRISDWNRVGWGEIPMSLGFTYSSNTLMMHLQDLVGADKMKSWYERF
                     GFGKSTKGMFDGEAPGQIGWSNELQQKTSSFGQSTTVTPVQMLQAQSAFFNDGNMLKP
                     WFVNSVENPVSKRQFYKGQKQIAGKPITKDTAEKVEKQLDLVVNSKKSHAANYRIDGY
                     EVEGKTGTAQVAAPNGGGYVKGPNPYFVSFMGDAPKKNPKVIVYAGMSLAQKNDQEAY
                     ELGVSKAFKPIMENTLKYLNVGKSKDDTSNAEYSKVPDVEGQDKQKAIDNVSAKSLEP
                     VTIGSGTQIKAQSIKAGNKVLPHSKVLLLTDGDLTMPDMSGWTKEDVIAFENLTNIKV
                     NLKGSGFVSHQSISKGQKLTEKDKIDVEFSSENVDSNSTNNSDSNSDDKKKSDSKTDK
                     DKSD"
     gene            7013..7978
                     /gene="mraY"
                     /locus_tag="ACGEXS_000771"
     CDS             7013..7978
                     /gene="mraY"
                     /locus_tag="ACGEXS_000771"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFF8011807.1"
                     /translation="MIFVYALLALVITFVLVPVLIPTLKRMKFGQSIREEGPQSHMKK
                     TGTPTMGGLTFLLSIVITSLVAIIFVDQANPIILLLFVTIGFGLIGFIDDYIIVVKKN
                     NQGLTSKQKFLAQIGIAIIFFVLSNVFHLVNFSTSIHIPFTNVAIPLSFAYVIFIVFW
                     QVGFSNAVNLTDGLDGLATGLSIIGFTMYAIMSFVLGETAIGIFCIIMLFALLGFLPY
                     NINPAKVFMGDTGSLALGGIFATISIMLNQELSLIFIGLVFVIETLSVMLQVASFKLT
                     GKRIFKMSPIHHHFELIGWSEWKVVTVFWAVGLISGLIGLWIGVH"
     gene            7980..9329
                     /gene="murD"
                     /locus_tag="ACGEXS_000772"
     CDS             7980..9329
                     /gene="murD"
                     /locus_tag="ACGEXS_000772"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFF8011808.1"
                     /translation="MLNYTGLENKNVLVVGLAKSGYEAAKLLSKLGANVTVNDGKDLS
                     QDAHAKDLESMGISVVSGSHPLTLLDNNPIIVKNPGIPYTVSIIDEAVKRGLKILTEV
                     ELSYLISEAPIIAVTGTNGKTTVTSLIGDMFKKSRLTGRLSGNIGYVASKVAQEVKPT
                     DYLVTELSSFQLLGIEKYKPHIAIITNIYSAHLDYHENLENYQNAKKQIYKNQTEEDY
                     LICNYHQRQVIESEELKAKTLYFSTQQEVDGIYIKDGFIVYKGVRIINTEDLVLPGEH
                     NLENILAAVLACILAGVPIKAIIDSLTTFSGIEHRLQYVGTNRTNKYYNDSKATNTLA
                     TQFALNSFNQPIIWLCGGLDRGNEFDELIPYMENVRAMVVFGQTKAKFAKLGNSQGKS
                     VIEANNVEDAVDKVQDIIEPNDVVLLSPACASWDQYSTFEERGEKFIERFRAHLPSY"
     gene            9345..10664
                     /locus_tag="ACGEXS_000773"
     CDS             9345..10664
                     /locus_tag="ACGEXS_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFF8011809.1"
                     /translation="MDDKTKNDQQESNEDKDELELFTRNTSKKRRQRKRSKATHFSNQ
                     NKDDTSQQADFDEEIYLINKDFKKEESNDKNNDSASSHANDNNIDDSTDSNIENEDYR
                     YNQEIDDQNESNVISVDNEQPQSAPKEQNSDSIDEETVTKKERKSKVTQLKPLTLEEK
                     RKLRRKRQKRIQYSVITILVLLIAVILIYMFSPLSKIAHVNINGNNHVSTSKINKVLG
                     VKNDSRMYTFSKKNAINDLEENPLIKSVEIHKQLPNTLNVDITENEIIALVKYKGKYL
                     PLLENGKLLKGSNDVKINDAPVMDGFKGTKEDDMIKALSEMTPEVRRYIAEVTYAPSK
                     NKQSRIELFTTDGLQVIGDISTISKKMKYYPQMSQSLSRDSSGKLKTRGYIDLSVGAS
                     FIPYRGNTSSQSESDKNVTKSSQEENQAKEELQSVLNKINKQSSKNN"
     gene            10770..12182
                     /gene="ftsA"
                     /locus_tag="ACGEXS_000774"
     CDS             10770..12182
                     /gene="ftsA"
                     /locus_tag="ACGEXS_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFF8011810.1"
                     /translation="MEEHYYVSIDIGSSSVKTIVGEKFHNGINVIGTGQTYTSGIKNG
                     LIDDFDIARQAIKDTIKKASIASGVDIKEVFLKLPIIGTEVYDESNEIDFYEDTEING
                     SHIEKVLEGIREKNDVQETEVINVFPIRFIVDKENEVSDPKELIARHSLKVEAGVIAI
                     QKSILINMIKCVEACGVDVLDVYSDAYNYGSILTATEKELGACVIDIGEDVTQVAFYE
                     RGELVDADSIEMAGRDITDDIAQGLNTSYETAEKVKHQYGHAFYDSASDQDIFTVEQV
                     DSDETVQYTQKDLSDFIEARVEEIFFEVFDVLQDLGLTKVNGGFIVTGGSANLLGVKE
                     LLSDMVSEKVRIHTPSQMGIRKPEFSSAISTISSSIAFDELLDYVTINYHDNEETEED
                     VIDVKDKDNESKLGGFDWFKRKTNKKDTHENEVESTDEEIYQSEDNHQEHKQNHEHVQ
                     DKDKDKEESKFKKLMKSLFE"
     gene            12215..13387
                     /gene="ftsZ"
                     /locus_tag="ACGEXS_000775"
     CDS             12215..13387
                     /gene="ftsZ"
                     /locus_tag="ACGEXS_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFF8011811.1"
                     /translation="MLEFEQGFNHLATLKVIGVGGGGNNAVNRMIDHGMNNVEFIAIN
                     TDGQALNLSKAESKIQIGEKLTRGLGAGANPEIGKKAAEESREQIEDAIQGADMVFVT
                     SGMGGGTGTGAAPVVAKIAKEMGALTVGVVTRPFSFEGRKRQTQAAAGVEAMKAAVDT
                     LIVIPNDRLLDIVDKSTPMMEAFKEADNVLRQGVQGISDLIAVSGEVNLDFADVKTIM
                     SNQGSALMGIGVSSGENRAVEAAKKAISSPLLETSIVGAQGVLMNITGGESLSLFEAQ
                     EAADIVQDAADEDVNMIFGTVINPELQDEIVVTVIATGFDDKPTSHGRKSGSTGFGTS
                     VNTSSNATSKDESFTSNSSNAQATDSVSERTHTTKEDDIPSFIRNREERRSRRTRR"
     gene            13647..14438
                     /gene="pgeF"
                     /locus_tag="ACGEXS_000776"
     CDS             13647..14438
                     /gene="pgeF"
                     /locus_tag="ACGEXS_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HFF8011812.1"
                     /translation="MNDNFKKQPHHLIYEELLQQGITLGITTRGDGLSDYPKNAFNMA
                     RYIDDRPYNITQHQLQLAEEIAFDRKNWVFPIQTHENKVACITKDDIGTNIDTLTDAL
                     HGIDAMYTYDSNVLLTMCYADCVPVYFYSTKHHFIALAHAGWRGTYTEIVKEVLKHVN
                     FDLKDLHVVIGPSTSSSYEINDDIKNKFETLPIDSANYIETRGRDRHGIDLKKANAAL
                     LIYYGVPKENIYTTAYATSEHLELFFSYRLEKGQTGRMLAFIGQQ"
     gene            14462..15130
                     /locus_tag="ACGEXS_000777"
     CDS             14462..15130
                     /locus_tag="ACGEXS_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFF8011813.1"
                     /translation="MKDNLQQISTQINDKSEKNNFSTKPNVIAVTKYVTIERAKEAYE
                     AGIRHFGENRLEGFFQKKEALPSDAVIHFIGSLQSRKVKDVINDVDYFHALDRLSLAK
                     EINKRAEHKIKCFLQVNVSGEASKHGIALEDVDQFIDDLKKYDKIEIVGLMTMAPLTD
                     DEAYIRSLFKQLRLKKEEIQRLNLEYAPCDELSMGMSNDYLIAVEEGATFVRIGTKLV
                     GEEE"
     gene            15127..15690
                     /locus_tag="ACGEXS_000778"
     CDS             15127..15690
                     /locus_tag="ACGEXS_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFF8011814.1"
                     /translation="MSHLALKDLFSGFFVIDDEEEVEVPDKQQQVNEAPAKEQSQQTT
                     KQNAIKSVPQKSASRYTTTSEERNNRMSNYSKNNSRNVVTMNNATPNNASQESSKMCL
                     FEPRVFSDTQDIADELKNRRATLVNLQRIDKVSAKRIIDFLSGTVYAIGGDIQRVGTD
                     IFLCTPDNVEVAGSITDHIENMEHSFD"
     gene            15702..15992
                     /locus_tag="ACGEXS_000779"
     CDS             15702..15992
                     /locus_tag="ACGEXS_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFF8011815.1"
                     /translation="MDINVLATIFKFILFVVEIYYFGMIIYFFTSWVPSIRETKVGYF
                     LAKIYEPFLQPFRKVIPPIGIIDISSIAAIIVLVLFQKGLLQIFNWILIQLQ"
     gene            16102..16881
                     /locus_tag="ACGEXS_000780"
     CDS             16102..16881
                     /locus_tag="ACGEXS_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFF8011816.1"
                     /translation="MINIDIYQHFRQEEYELIDQLTDKCDQAEQHYAPVLTHFLDPRG
                     QYILEVICGSYEDLNVSFYGGPNAERKRAIISPNYYEPKESDFELTLMEIDYPEKFVT
                     LKHQHILGTLMSLGIEREQVGDIIVNERIQFVLTSRLESFIMLELQRIKGASVKLYTI
                     PVTDMIQSNENWKNESATVSSLRLDVVIKEMIRKSRTIAKQLIEKKRVKVNHTIVDSA
                     DFQLQANDLISIQGFGRAHITDLGGKTKKDKTHITYRTLFK"
     gene            16905..17522
                     /locus_tag="ACGEXS_000781"
     CDS             16905..17522
                     /locus_tag="ACGEXS_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFF8011817.1"
                     /translation="MPFTPNEIKNKEFSRVKNGLEPTEVANFLEQLSTEIERLKEDKK
                     QLEKVIEERDTNIKSYQDVHQSVSDALIQAQKAGEETKQAAEKQAEAITAKAEAQANQ
                     MVGDAVEKARRLAFQTEDMKRQSKVFRSRFRMLVEAQLDLLKNEDWDYLLNYDLDAEQ
                     VTLENIHHLHENDLKPDEVAANAQNNASNTPDNNQQSNDSETTKK"
     gene            17743..20496
                     /gene="ileS"
                     /locus_tag="ACGEXS_000782"
     CDS             17743..20496
                     /gene="ileS"
                     /locus_tag="ACGEXS_000782"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFF8011818.1"
                     /translation="MDYKETLLMPKTDFPMRGGLPNKEPQIQEKWDAEDQYHKALEKN
                     KGNETFILHDGPPYANGNLHMGHALNKILKDFIVRYKTMQGFYAPYVPGWDTHGLPIE
                     QALTKKGVDRKKMSTAEFREKCKEFALEQIELQKKDFRRLGVRGDFNDPYITLKPEYE
                     AAQIRIFGEMADKGLIYKGKKPVYWSPSSESSLAEAEIEYHDKRSASIYVAFDVKDDK
                     GVVDADAKFIIWTTTPWTIPSNVAITVHPELKYGQYNVNGEKYIIAEALSDAVAEALD
                     WDKASIKLEKEYTGKELEYVVAQHPFLDRESLVINGDHVTTDAGTGCVHTAPGHGEDD
                     YIVGQKYELPVISPIDDKGVFTEEGGQFEGMFYDKANKAVTDLLTEKGALLKLDFITH
                     SYPHDWRTKKPVIFRATPQWFASISKVRQDILDAIENTNFKVNWGKTRIYNMVRDRGE
                     WVISRQRVWGVPLPVFYAENGEIIMTKETVNHVADLFAEHGSNIWFEREAKDLLPEGF
                     THPGSPNGTFTKETDIMDVWFDSGSSHRGVLETRPELSFPADMYLEGSDQYRGWFNSS
                     ITTSVATRGVSPYKFLLSHGFVMDGEGKKMSKSLGNVIVPDQVVKQKGADIARLWVSS
                     TDYLADVRISDEILKQTSDVYRKIRNTLRFMLGNINDFNPDTDSIPESELLEVDRYLL
                     NRLREFTASTINNYENFDYLNIYQEVQNFINVELSNFYLDYGKDILYIEQRDSHIRRS
                     MQTVLYQILVDMTKLLAPILVHTAEEVWSHTPHVKEESVHLADMPKVVEVDQALLDKW
                     RTFMNLRDDVNRALETARNEKVIGKSLEAKVTIASNDKFNASEFLTSFDALHQLFIVS
                     QVKVVDKLDDQATAYEHGDIVIEHADGEKCERCWNYSEDLGAVDELTHLCPRCQQVVK
                     SLV"
     gene            20786..21583
                     /locus_tag="ACGEXS_000783"
     CDS             20786..21583
                     /locus_tag="ACGEXS_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFF8011819.1"
                     /translation="MYHNSNANFVNGITLNVRDKNELKPFYEDILGLNIINETLTSIQ
                     YEVGQNNHVITLVELQNGREPLMSEAGLFHIAIKLPQISDLANLLIHLSEYDIPVNGG
                     IQPASLSLFFEDPEGNGFKFYVDKDEAQWTRQNNLVKIDIRPLNVPRLVSHATKLLWL
                     GIPDDAIIGALHIKTIHLSEVKEYYLDYFGLEQSAYMDDYSIFLASNGYYQHLAMNDW
                     VSATKRVENFDTYGLAIVDFHYPETTHLNLQGPDGIYYRFNHIEVED"
BASE COUNT         8004 a         3004 c         4154 g         6600 t
ORIGIN      
        1 ttttaattat gtttttaatg atattggtga ggatgaaaga cagcactcga ttatagttgg
       61 agattcttta acatctgaca ttctaggtgg aatcaatgcg ggtatagcta cttgctggtt
      121 taattttaga ggatttgatc ataatccagg aattatacct gattatgaaa ttaattcatg
      181 gaaacaacta aatgatattg tacgttaaga caaataattt gaaaatgatt tacttatatt
      241 agcttaagaa agtaattgta aaaaagacac tacgaaaaga tgcattttca tgctcgtagt
      301 gtcgaatatt attatcttat ggtttccaca ataagacatc atgtccctct atatttttag
      361 cttccacatc tgtaaatcca tgatgtaaaa agaaatcctt agaatcattt ctgccaatgg
      421 ctttaattgg catattgaaa ctctttgcaa aatcaatcaa ttctgaagcg taccctctgt
      481 tttgatattt tggtaatact tctaacttcc ataataatat ataatcttca aaatctggga
      541 agtagatttc ttcgacatca ccttttttta gtaatgccat tctagctcct aattgatctc
      601 cgacaaatat gccataaaat ggtgaatctg aacttgcatc aatcatttga ccgtttaact
      661 cattgaccat gtataagtct ttgttgccaa acgctctaaa gttttcgaat aattcgtcag
      721 ttttgtaatt aatttcaaga cgtttgattt cactcatatt tatctacccc ttatttgttt
      781 actactatta ttatacatta aaccacttgt gtttttcatt gatttgaaaa tgtaaaacaa
      841 tgaatattat aaattttcca tgattaaatt gattttcaag ccgtaaaatt gtcaatcacc
      901 ggtatagact ttatactata gtttgtaatc gcgtacttaa ggagaaaaaa catggactgt
      961 aaagtagtta gtttaaatga aaaagatcag tttataccaa aaataaagag cagtgaccct
     1021 gtaataacag gattatttca atatgatgca gctcaacaaa ctagttttga aaaaaggatg
     1081 tctaaagaaa ataatggaag agaagcggca ttagcgaatg ttattcgtga atatatgagt
     1141 gatttaaagc tttcaagtga acaagaatta aacatacaac atttagctaa tggttcaaaa
     1201 gttgtgattg gtggacaaca agcagggctt ttcgggggac cattgtatac attccataaa
     1261 atattttcaa tcattacttt atctaaggaa ttaacggata cacataagca acaagtagta
     1321 ccagtttttt ggattgcagg agaagatcat gatttcgatg aagtgaatca tacatttgtt
     1381 tataacgaaa atcatgggtc gctgcataag gttaaatatc atacaatgga gatgccagag
     1441 acgactgtct ctagatatta tcctgataag gctgagttga aacaaacttt aaaaacgatg
     1501 ttcattcata tgaaagaaac tgttcataca caaggtctac tggagatttg tgacagaatt
     1561 attgaccaat atgactcgtg gactgatatg tttaaagcac tactgcatga aacatttaaa
     1621 gcatatggcg ttctatttat agatgcgcag tttgagccgt taagaaaaat ggaagcgcct
     1681 atgtttaaaa agattttgaa aaaacatcag ttgcttgatg atgcttttag agcaacacaa
     1741 caacgtactc aaaatcaagg cttgaatgcg atgatacaaa cagatacaaa tgttcattta
     1801 ttcttacatg atgaaaatat gcgtcaatta gtttcgtatg atggtaagca ttttaaatta
     1861 aataaaacag ataagacata tataaaggaa gaaattataa atattgcgga aaatcaacct
     1921 gaattatttt ctaataatgt agtgacaaga ccattaatgg aagaatggtt atttaacacg
     1981 gtggcatttg ttggaggacc gagtgaaatt aagtactggg ctgaactaaa agatgtattt
     2041 gaactatttg atgttgaaat gcctatcgtg atgccaaggc ttagaattac ttatttaaat
     2101 gaccgtatag aaaaattact ttcgaaatac aatattccat tagaaaaagt gttagtcgat
     2161 ggtgttgaag gagaaagaag taagtttatt agagaacaag catcacatca atttattgaa
     2221 aaggtagaag gtatgattga acaacagcgt cgtctaaaca aagacttatt agatgaagtg
     2281 gcggggaatc aaaataatat taaccttgtg aataaaaata atgaaattca tatacaacag
     2341 tatgattatt tgttaaaacg ttatctttta aacattgaaa gagaaaacga catcagtatg
     2401 aagcaattta gagaaattca agaaacactc catccaatgg gaggattaca agaaagaata
     2461 tggaatccac ttcaaatttt gaatgatttt gggacagatg tgttcaagcc ctccacctat
     2521 ccaccacttt cttacacttt tgatcatatt attataaaac cttaatatac caagggttta
     2581 gcccgattta tcttaatgat aaatcgggca tttttttgtt ttttaaaata aatttcacaa
     2641 attttgtata aatagtggtg gatagtgggg agatgtggta aattatatat aaggtgaggt
     2701 gataaaaaat gttcatggga gaatacgatc atcaattaga tacaaaagga cgtatgatta
     2761 taccgtccaa gtttcgttat gacttaaatg agcgttttat tatcacaaga ggccttgata
     2821 aatgtttatt cggttacact ctagacgaat ggcaacagat tgaagagaaa atgaaaacct
     2881 tacctatgac aaaaaaagac gcacgtaagt ttatgcgtat gttcttctct ggtgctgttg
     2941 aagtagaact tgataagcaa gggcgtatta acatccctca aaacttgagg aaatacgcta
     3001 atttaactaa agaatgtaca gtaatcggtg tttcaaatcg tattgagatt tgggatagag
     3061 aaacttggaa tgatttctat gaagaatctg aagaaagttt cgaagatatt gctgaagatt
     3121 taatagattt tgatttttaa aatggaggaa ttgaagtgtt tcatcatatc agcgttatgt
     3181 taaacgaaac cattgattat ttaaatgtaa aagaaaatgg tgtgtacatt gactgtacgc
     3241 taggtggagc gggacatgcc ctttatttac taaatcaatt aaatgacgac ggaagattaa
     3301 tagcaatcga tcaagaccaa actgcaattg ataatgctaa agaggtatta aaggatcatt
     3361 tgcataaggt gacttttgtt catagcaact tccgtgaatt aactcaaata ttaaaagact
     3421 taaacattga aaaagtagat ggaatttatt acgacttggg tgtttcaagc ccacaactcg
     3481 acattccaga acgaggattc agttatcacc atgacgcaac attagacatg cgtatggacc
     3541 aaacacaaga actaacagca tatgaaattg ttaacaattg gtcatatgaa gcgttagtga
     3601 agatttttta tcgctatggc gaggagaaat tttcaaaaca gatagctcga agaatcgaag
     3661 cacatcgcga acaacaacca ataacaacaa cattagaatt agttgacatt ataaaagaag
     3721 gtattcctgc aaaagcaaga agaaaaggcg gacatcctgc aaaacgagta tttcaagcac
     3781 tacgaattgc agtaaacgat gaattgtcag cttttgaaga ttcaatagaa caagcgattg
     3841 aattagtgaa agtagatggc aggatttcgg taatcacttt ccattcttta gaagatcgtt
     3901 tatgtaaaca ggtgttccaa gaatatgaaa aaggtccaga ggtaccaaga ggattaccag
     3961 ttataccaga agcatataca cctaagttaa agcgtgttaa tcgtaaaccg attaccgcta
     4021 cagaagaaga tttagatgac aataacagag cacgaagcgc gaaattacgt gtagctgaaa
     4081 tacttaaata aggagcaatt tataatggct gtagaaaaag tgtaccaacc atatgacgaa
     4141 caagtttata atagtatacc gaagcaacaa ccacaaacta agcccgaaaa gaagtctgtt
     4201 tcgagaaaag tggttgtaca attaactaaa tttgaaaaag ttttatacat aactttgatt
     4261 actgtaattg ctatgttaag tatttatatg ctatctttaa aaatggatgc gtatgatacg
     4321 cgaggaaaga ttgcagattt agattataaa atagataaac aatcaagtga aaacagtgct
     4381 ttacaatctg aaatcaaaaa gaattcttct tatgaacgca tatacgaaaa ggctaagaaa
     4441 caggggatga gccttgagaa cgataatgta aaggtagtgc gtagtaatgg cgaagcaaaa
     4501 aattaaaatt aaaaaaaata aaataggggc agtcctactt gttggtttat tcggactgct
     4561 cttttttata ttggttttaa gaatttcata tatcatgatt actggacatt ctaatggtca
     4621 agatttagtc atgaaggcaa atgaaaagta tttagttaag aatgcacaac aaccagaacg
     4681 aggaaagata tatgatcgta atggtaaagt gctagcagaa gatgtagaaa gatataaact
     4741 tgttgcagta atagataaaa aggcgagtgc caattctaaa aaacctaggc atgtagttga
     4801 taaaaaagag actgcaaaga aattatctac agtcattaat atgaagccag aggaaattga
     4861 aaagagactt agtcaaaaga aagctttcca aattgaattt ggacgcaaag gaacaaattt
     4921 aacgtatcag gacaaattga aaatagagaa aatgaatttg cctggtattt ctttattgcc
     4981 tgaaacagaa cgcttttatc caaatggcaa ttttgcatca cacttaattg gtagagctca
     5041 gaaaaatccg gatactggtg aacttaaagg tgcacttgga gttgaaaaga tttttgatag
     5101 ttatttaagt ggatctaaag gatcattgag atatattcat gatatttggg gatatatcgc
     5161 accaaatact aaaaaagaga agcagcctaa acgtggtgat gatgtccatt taacaatcga
     5221 ttcaaatatt caagtatttg ttgaagaagc tttagatggc atggttgaaa gataccagcc
     5281 gaaagattta tttgcggttg tcatggatgc caaaactgga gaaattttag catacagtca
     5341 gcgaccaaca tttaatcctg aaactggtaa agactttggt aaaaagtggg caaatgacct
     5401 ttatcaaaac acatacgagc ctggatcaac atttaaatca tatgggttag cagctgctat
     5461 tcaagaaggt gcttttgatc ctgataagaa atataaatct ggacatagag atattatggg
     5521 ttcacgtatt tcagactgga atagagtcgg ttggggtgaa atcccaatgt cactcggatt
     5581 tacttattca tctaatacat tgatgatgca tttacaagat ttagttggtg cagacaaaat
     5641 gaaatcttgg tatgaacgat ttggatttgg aaaatcaact aaaggtatgt ttgatggaga
     5701 agcacctggt caaattggat ggagtaatga gttgcaacaa aaaacgtcat catttggtca
     5761 atcgacaaca gtaacacctg ttcaaatgtt acaagcgcaa tcagcgttct ttaatgatgg
     5821 taatatgtta aaaccatggt ttgtgaatag cgttgaaaat cctgttagta aaagacaatt
     5881 ttataaaggg caaaaacaaa tcgcaggcaa accaataaca aaagatactg ctgaaaaagt
     5941 tgaaaagcaa ttggatttag ttgtgaatag taagaagagt cacgctgcaa actatcgtat
     6001 tgatggttat gaggtcgaag gtaagactgg tacagcacaa gtcgctgcac ctaatggtgg
     6061 tggatacgtt aaaggtccaa acccatattt tgtaagtttt atgggtgacg cgccgaagaa
     6121 aaatcctaaa gttattgtat acgctggtat gagcttggca caaaaaaatg accaagaagc
     6181 ttatgaatta ggtgttagta aagcgtttaa accaataatg gaaaatactt tgaaatattt
     6241 aaatgtaggt aaatcaaaag atgacacatc taatgcagag tatagtaaag tgccagatgt
     6301 tgaaggtcaa gacaaacaaa aagctattga taatgtgagt gcaaaatcat tagaaccagt
     6361 tactattggt tctggcacac aaataaaagc acaatctata aaagcaggga ataaagtctt
     6421 acctcatagt aaagtactgt tattaacaga tggagactta actatgcctg acatgtcagg
     6481 atggacgaaa gaagatgtca ttgcttttga aaacctaaca aatattaaag taaatttaaa
     6541 aggtagcggt tttgtgtccc accaatcaat tagtaaggga caaaaactta ctgaaaaaga
     6601 taaaatagac gtagaatttt catcagagaa tgtagacagc aattcgacga ataattctga
     6661 ttcaaattca gatgataaga agaaatctga cagtaaaact gacaaggata agtcggacta
     6721 acaagaatgg ctacattaaa ttaggatgta ttgtcactat ttgtttttac taaagtaatg
     6781 ctgtgataga aaacaacatc ttcatttaat ctttataaat aagtgttgat agagaatttt
     6841 aaggtagacg aggtcagatt aacttgtttg ttataggcaa actttttata ctggcctctt
     6901 atgccgtaaa aaagaaaaat tataaatttt gattacaact ttgtttattt aattgaaaaa
     6961 ttgataatat attagtggtt aaaaaagtct ggattaagaa gaggagatag ttatgatttt
     7021 tgtatatgcg ttattagcgc tagtgattac atttgttttg gtacctgttt taatacctac
     7081 attaaaaagg atgaaatttg gtcaaagtat tcgagaagaa ggtccacaaa gccatatgaa
     7141 gaagactggt acaccaacga tgggtggact aacatttcta ttaagtattg tgataacgtc
     7201 tttggtggct attatatttg tagatcaagc taatccaatc atactgttat tatttgtgac
     7261 gattggtttt gggttaattg gttttataga tgattatatt attgttgtta aaaagaataa
     7321 ccaaggttta acaagtaaac agaagttttt ggcgcaaatt ggtattgcga ttatattctt
     7381 tgttttaagt aatgtgtttc atttggtgaa tttttctacg agcatacata ttccatttac
     7441 gaatgtagca atcccactat catttgcata tgttattttc attgtttttt ggcaagtagg
     7501 tttttctaat gcagtaaatt taacagatgg tttagatgga ttagcaactg gactgtcaat
     7561 tatcggattt acaatgtatg ccatcatgag ctttgtgtta ggagaaacgg caattggtat
     7621 tttctgtatc attatgttgt ttgcactttt aggattttta ccatataaca ttaaccctgc
     7681 taaagtgttt atgggagata caggtagctt agctttaggt ggtatatttg ctaccatttc
     7741 aatcatgctt aatcaggaat tatcattaat ttttataggt ttagtattcg taattgaaac
     7801 attatctgtt atgttacaag tcgctagctt taaattgact ggaaagcgta tatttaaaat
     7861 gagtccgatt catcatcatt ttgaattgat aggatggagc gaatggaaag tagttacagt
     7921 attttgggct gttggtctga tttcaggttt aatcggttta tggattggag tgcattaaga
     7981 tgcttaatta tacagggtta gaaaataaaa atgtattagt tgtcggtttg gcaaaaagtg
     8041 gttatgaagc agctaaatta ttaagtaaat taggtgcgaa tgtaactgtc aatgatggaa
     8101 aagacttatc acaagatgct catgcaaaag atttagaatc tatgggcatt tctgttgtaa
     8161 gtggaagtca tccattaacg ttgcttgata ataatccaat aattgttaaa aatcctggaa
     8221 taccttatac agtatctatt attgatgaag cagtgaaacg aggtttgaaa attttaacag
     8281 aagttgagtt aagttatcta atctctgaag caccaatcat agctgtaacg ggtacaaatg
     8341 gtaaaacgac agttacttct ctaattggag atatgtttaa aaaaagtcgc ttaactggaa
     8401 gattatccgg caatattggt tatgttgcat ctaaagtagc acaagaagta aagcctacag
     8461 attatttagt tacagagttg tcgtcattcc agttacttgg aatcgaaaag tataaaccac
     8521 acattgctat aattactaac atttattcgg cgcatctaga ttaccatgaa aatttagaaa
     8581 actatcaaaa tgctaaaaag caaatatata aaaatcaaac ggaagaggat tatttgattt
     8641 gtaattatca tcaaagacaa gtgatagagt cggaagaatt aaaagctaag acattgtatt
     8701 tctcaactca acaagaagtt gatggtattt atattaaaga tggttttatc gtttataaag
     8761 gtgttcgtat tattaacact gaagatctag tattgcctgg tgaacataat ttagaaaata
     8821 tattagcagc tgtgcttgct tgtattttag ctggtgtacc tattaaagca attattgata
     8881 gtttaactac attttcagga atagagcata gattgcaata tgttggtact aatagaacta
     8941 ataaatatta taatgattcc aaagcaacaa acacgctagc aacacagttt gccttaaatt
     9001 catttaatca accaatcatt tggttatgtg gtggtttgga tcgagggaat gaatttgacg
     9061 aactcattcc ttatatggaa aatgttcgcg cgatggttgt attcggacaa acgaaagcta
     9121 agtttgctaa actaggtaat agtcaaggga aatcggtcat tgaagcgaac aatgtcgaag
     9181 acgctgttga taaagtacaa gatattatag aaccaaatga tgttgtatta ttgtcacctg
     9241 cttgtgcgag ttgggatcaa tatagtactt ttgaagagcg tggagagaaa tttattgaaa
     9301 gattccgtgc ccatttacca tcttattaaa gggtgtgagt attgatggat gataaaacga
     9361 agaacgatca acaagaatca aatgaagata aagatgaatt agaattattt acgaggaata
     9421 catctaagaa aagacggcaa agaaaaagat caaaggctac acatttttct aatcaaaata
     9481 aagatgatac atctcaacaa gctgattttg atgaagaaat ttacttgata aataaagact
     9541 tcaaaaaaga agaaagcaat gataaaaata atgattctgc ttctagtcat gcgaatgata
     9601 ataatatcga tgattctaca gactctaata ttgaaaatga ggattataga tataatcaag
     9661 aaattgacga ccaaaatgaa tcgaatgtaa tttcagtcga caacgaacaa cctcaatcag
     9721 ctcctaaaga acaaaatagc gactcgattg atgaggaaac agtaacgaaa aaagaacgaa
     9781 aaagtaaagt aacacaatta aagccattaa cacttgaaga aaagcggaag ttaagacgta
     9841 agcgacaaaa gcgaatccaa tacagtgtta ttacaatatt ggtattgttg attgctgtta
     9901 tattaattta catgttttca ccacttagta aaattgcgca tgtaaatata aatggaaata
     9961 atcacgttag tacttcaaag ataaacaaag ttttaggtgt taaaaatgat tcaaggatgt
    10021 atacgtttag taaaaaaaat gctattaatg atctcgaaga gaatccatta atcaaaagtg
    10081 ttgagataca caagcaatta ccaaacacat taaacgtaga tatcacagaa aatgaaatta
    10141 ttgctttagt gaaatataaa ggtaaatatt tacctttatt agaaaatggt aaattgctta
    10201 aaggttcaaa tgatgtcaaa attaatgatg cacctgtcat ggatggtttc aaaggtacaa
    10261 aagaagatga tatgattaag gcgttatctg aaatgacacc tgaagttaga cgatatattg
    10321 ccgaagtgac atacgcccca agtaaaaaca aacaaagcag aattgaattg tttacgacag
    10381 atggacttca agtaatcggt gatatttcga cgatatctaa gaaaatgaaa tattatccgc
    10441 agatgtcaca atcattatca agggatagtt cgggtaaact aaaaacacga ggctatattg
    10501 atttatcagt cggtgcttca tttatcccat accgtggaaa cacgtctagt caatcagaaa
    10561 gcgataaaaa tgtgactaaa tcatctcaag aggaaaatca agcaaaagaa gaattacaaa
    10621 gcgttttaaa caaaattaac aaacaatcaa gtaagaataa ttaaaaaatt ttgatattgt
    10681 ctatgtttat agttcacaag ccattcaacg tattgtaaac taaggatagt gtattttttt
    10741 aatagtaatt tgtcaggagg tgcctatcta tggaagaaca ttactacgta agtattgata
    10801 ttggatcatc aagcgtaaaa acaatagtag gcgagaaatt tcacaatggt ataaatgtga
    10861 taggtacagg acaaacctac acgagcggta taaaaaatgg tttaattgat gattttgata
    10921 ttgcgcgaca agcaatcaaa gacacaatta aaaaggcatc aatcgcttcg ggtgttgata
    10981 ttaaagaagt tttcctgaaa ttacctatca ttggaacgga agtttatgat gaatcaaatg
    11041 aaatcgactt ttatgaggat acagaaatca acggttcaca tatcgaaaaa gtattagaag
    11101 gtattagaga aaaaaatgat gtgcaagaaa cagaagtaat taatgtgttc ccgattcgtt
    11161 ttatagtcga taaagaaaat gaggtttcag accctaaaga attaattgcc agacattcat
    11221 taaaggttga agcaggcgta attgctattc aaaaatcgat tttaattaat atgattaaat
    11281 gcgtagaagc atgtggtgtt gatgtattag atgtttactc tgatgcatat aactatggtt
    11341 caatcctaac agctactgaa aaagagttag gtgcatgtgt cattgatatt ggtgaagacg
    11401 ttacgcaagt tgctttttat gaacgcggtg aattagtaga tgctgattct atcgaaatgg
    11461 cagggcgtga tattacagac gatattgcac aaggattaaa cacttcttat gaaactgctg
    11521 aaaaagttaa acaccaatat ggtcatgcat tctatgattc tgcttcagat caagatatct
    11581 tcactgttga acaggttgat agtgatgaaa cagtacagta tactcaaaaa gatttgagtg
    11641 actttattga agcgcgtgta gaagaaatat tcttcgaagt atttgatgtt ttacaagatt
    11701 taggattaac aaaagtaaat ggtgggttta ttgtaactgg tggatctgca aacttacttg
    11761 gcgtaaaaga attattatca gatatggtaa gtgaaaaagt tagaattcac acgccatcac
    11821 aaatgggaat tagaaaacct gaattttctt cagcaatttc tacaatttct agtagtatcg
    11881 cttttgatga gttattagat tatgttacaa ttaattatca tgataatgaa gaaactgaag
    11941 aagatgttat tgatgtgaaa gacaaagata acgaatctaa attaggcgga tttgattggt
    12001 ttaaacgtaa aacaaacaaa aaagatactc atgaaaatga agtagagtca acagatgaag
    12061 aaatttatca atcagaagat aatcatcagg aacataaaca gaatcatgaa catgttcaag
    12121 acaaagataa agataaagaa gaaagtaaat tcaaaaaact aatgaaatct ctatttgaat
    12181 gattattggc caataaaact aggaggaaat ttaaatgtta gaatttgaac aaggatttaa
    12241 tcatttagcg actttaaagg tcattggtgt aggtggtggc ggtaacaacg ccgtaaaccg
    12301 aatgattgac cacggaatga ataatgttga atttatcgct atcaacacag acggtcaagc
    12361 tttaaactta tctaaagctg aatctaaaat ccaaatcggt gaaaaattaa cacgtggttt
    12421 aggagcagga gctaatcctg aaatcggtaa aaaagctgca gaggaatctc gtgaacaaat
    12481 tgaagatgca atccaaggtg cagacatggt atttgttact tctggtatgg gtggcggaac
    12541 tggtactggt gcagcaccag tcgttgctaa aattgcaaaa gaaatgggcg cattaactgt
    12601 tggtgttgta actcgtccat ttagttttga aggacgtaag cgtcaaactc aagctgctgc
    12661 tggagtagaa gctatgaaag ctgcagtaga tacattaatc gttataccaa atgaccgttt
    12721 attagatatc gttgacaaat ctacgccaat gatggaagca tttaaagaag ctgacaacgt
    12781 gttacgccaa ggtgtacaag gtatctcaga cttaatcgct gtttctggtg aagtaaactt
    12841 agactttgca gacgttaaga caattatgtc taaccaaggt tctgcattaa tgggtattgg
    12901 tgtttcttct ggtgaaaata gagcggtaga agctgctaaa aaagcaatct cttctccatt
    12961 acttgaaaca tctatcgttg gtgcacaagg tgtgcttatg aatattactg gtggcgagtc
    13021 attgtcatta tttgaagcac aagaggctgc tgatattgtc caagatgctg cagatgaaga
    13081 cgttaatatg attttcggta cagttattaa tcctgaatta caagatgaga ttgttgtaac
    13141 agttattgca actggttttg atgacaaacc aacatcacat ggtcgtaaat ctggtagcac
    13201 tggattcgga acaagcgtaa atacttctag caatgcaact tctaaagatg aatcattcac
    13261 ttcaaattca tcaaatgcac aagcaactga tagtgtaagt gaaagaacac atacaactaa
    13321 agaagatgat attcctagct tcattagaaa tagagaagaa agacgttcaa gaagaacaag
    13381 acgttaatcg gttaatatat atacacaaat aattcaacac aaatcatcag ataacatatc
    13441 tgatgatttt tttactaatt tttagaacat gtagaaggac atttaagttt ttcaaagtta
    13501 ttaaaagtgt ttaagtatcg tgtgaaaatt aagtcaaaaa ttatttgcgc aacattttaa
    13561 ctttaaacat aaatgttata ttatataatt attaactttg tacagttaga cgaagataat
    13621 ttaaatgaaa tgatggtgac gatcgagtga atgataattt taaaaagcaa ccgcatcatt
    13681 taatatatga agagttatta caacaaggta ttactctagg tattacaact agaggagatg
    13741 gtttaagtga ctatcctaaa aatgctttta atatggcgag atatattgat gatcgcccat
    13801 ataatattac tcaacatcaa ttgcaattag ctgaagaaat tgcgtttgat agaaaaaatt
    13861 gggtgtttcc cattcaaaca catgaaaata aagtcgcttg tattacaaag gatgatatag
    13921 gcacaaatat agacacttta actgatgcgc ttcatggtat tgatgcgatg tacacatatg
    13981 atagtaatgt cttattaacg atgtgttatg cagactgtgt accagtatat ttttatagta
    14041 caaaacatca ttttattgca ttggcgcatg caggttggcg tggtacctat actgaaattg
    14101 taaaagaagt gctaaaacat gtgaactttg atttgaaaga cttacatgtc gttattggac
    14161 catctacatc atcaagttat gaaattaatg atgatattaa aaataaattt gaaacattgc
    14221 caattgatag tgccaactat attgaaacta gaggacgaga tcgtcatggt attgatttga
    14281 aaaaagccaa tgctgcatta ttaatttatt atggtgttcc taaagaaaat atttatacga
    14341 cagcgtatgc tacatctgaa catttagaat tatttttctc ttatcgatta gaaaaaggtc
    14401 aaacaggacg catgttagca ttcattggtc aacagtaaac aaggaggaga tatgtttgcg
    14461 tgtgaaagat aatttacaac aaatctcaac acaaattaat gacaaaagtg aaaaaaataa
    14521 tttttcaaca aaaccaaacg tgattgcagt tacaaaatat gttacaatag agcgagctaa
    14581 agaagcgtat gaggctggaa taagacattt tggtgagaat agattggaag gcttttttca
    14641 aaagaaagaa gcattaccat cagatgcggt gatccatttt ataggatcat tacaatctcg
    14701 aaaagttaag gacgttataa acgacgtaga ttatttccat gctttagatc gattgagctt
    14761 agccaaagaa attaacaaac gtgcagaaca taaaattaaa tgtttcttgc aagtgaacgt
    14821 ttcgggagaa gcttctaaac atggtattgc tttagaagat gttgatcagt ttatagatga
    14881 tcttaaaaaa tatgacaaaa tcgaaattgt aggtttaatg acgatggcac cattgacaga
    14941 tgatgaagca tatattagat cgttatttaa acagttacgt ttgaaaaaag aagaaataca
    15001 acgactcaat ttagaatatg cgccttgtga tgaattatca atgggaatga gtaatgacta
    15061 tcttattgca gttgaagaag gtgcgacgtt tgttagaatt gggactaaac ttgtaggaga
    15121 agaggagtga gccacttggc tttaaaagat ttatttagtg gattttttgt aatagatgat
    15181 gaagaggaag tagaagtacc tgacaaacaa caacaggtaa atgaagcgcc agcaaaagag
    15241 cagtcacaac aaacaacaaa acaaaacgca atcaaatcag tccctcaaaa atctgcatca
    15301 agatatacaa caacgtcaga agaaaggaat aaccgtatgt ctaattattc aaaaaataat
    15361 tcacgtaatg ttgtaactat gaacaatgct acaccaaaca atgcatcaca agaaagttca
    15421 aaaatgtgtt tattcgaacc acgtgttttt tcagatacac aagatattgc tgatgagctt
    15481 aaaaaccgcc gtgcgacact tgtcaattta caacgtattg ataaagtatc agcgaaaaga
    15541 attattgatt ttttaagcgg tactgtttat gcaatcggtg gagatatcca acgtgtaggt
    15601 actgatattt tcttatgtac gcctgataat gtggaagtag ctggaagcat tacagaccat
    15661 attgaaaata tggaacattc attcgactaa ggagttaaca tatggatata aatgtgctag
    15721 ctacaatatt taaattcatc ctttttgttg ttgaaattta ttatttcggc atgattatat
    15781 atttctttac atcttgggta ccaagtatta gagaaactaa ggtaggttat tttttagcga
    15841 aaatatatga acctttctta caaccattta gaaaagtaat tccacctatt ggaattatcg
    15901 acatatcatc aatcgctgca attatcgttt tagtattatt ccaaaaaggg ttactccaaa
    15961 tctttaattg gattttaatt caattacaat aaattaagcg acataacgag gcaagggtac
    16021 atgatacttt agcctcgttt ttgatatgta tttttctgaa tataagggca atagatggta
    16081 ttttataatt tttttaaggt agtgattaac atagatattt atcaacactt tagacaggaa
    16141 gaatacgaat taattgatca gctaacggat aaatgtgatc aagcggaaca gcattatgca
    16201 ccagtattaa cgcatttttt agatccaaga gggcaatata tattggaagt gatttgtggc
    16261 agttatgaag atttaaacgt atctttttat ggtggaccta atgctgaaag aaaaagagca
    16321 atcatttcgc cgaactatta tgaacctaaa gaaagcgact ttgaattaac tttaatggaa
    16381 atagattatc ctgaaaaatt cgtcacttta aaacatcaac atattttagg gacattaatg
    16441 tctttaggta tcgaacgcga acaagttgga gatataattg tgaatgaacg aattcaattt
    16501 gttttgacaa gtagattgga atcatttatt atgttagaat tacaacgtat taaaggcgca
    16561 tcagttaaac tttatactat tccagtaaca gatatgatac aatctaatga gaattggaaa
    16621 aatgaaagtg caacagttag ttctttaagg ttagatgttg ttattaaaga aatgatacgt
    16681 aaatcacgta cgattgcgaa acaactaatc gaaaaaaaac gtgttaaagt gaatcacact
    16741 attgttgatt cagcagattt tcaattacaa gcaaatgatt taatatccat ccaaggtttt
    16801 ggtagagcac acattactga cttaggtggt aaaactaaaa aagataaaac gcacattacc
    16861 tatagaacat tattcaaata gtaatgattt aaggaggata acaaatgcct tttacaccaa
    16921 atgaaattaa gaataaagag ttttcacgtg taaagaatgg tttagaacct actgaagttg
    16981 ctaatttttt ggagcaacta agcactgaaa ttgaacgtct taaagaagat aaaaaacaac
    17041 ttgaaaaagt aatcgaagag agagatacta atattaagtc ttatcaagac gtgcatcaat
    17101 ctgtaagtga tgctttgata caagctcaaa aagctggtga agaaactaag caagctgcag
    17161 agaaacaagc tgaagcgatt acagctaagg cagaagcgca agctaatcaa atggttggtg
    17221 acgcggtaga aaaagcacgc cgtttagcat tccagactga agatatgaaa cgtcaatcaa
    17281 aagtatttag atcgcgtttc cgtatgttag ttgaagcgca attagactta ttaaaaaacg
    17341 aagattggga ttacttgttg aattatgatt tagacgctga acaagtgacg cttgaaaata
    17401 ttcatcattt gcatgaaaat gatttaaagc cagatgaagt tgcagcaaat gcacaaaata
    17461 atgcatcaaa tacaccagac aataatcaac aatccaatga ttcagaaaca actaagaagt
    17521 aagaattaaa taaagacaga cgcgtaatat acatttaact tttcacagcg aattaggtaa
    17581 tggtgagagc ctagtaaaag catgtatgtt atatcactgg ctttttaata tttaaataat
    17641 gtaatgagag aactctaagt tgagttaata agggtggtac cgcgagcaat cgtccctttt
    17701 aatttaactt agagtttttt aaatttttaa ggagtgaaaa aaatggatta caaagaaacg
    17761 ttattaatgc ctaaaacaga tttcccaatg cgaggtggtt taccaaacaa ggaaccgcaa
    17821 attcaagaaa aatgggatgc agaagatcaa taccataaag cgttagaaaa aaataaaggt
    17881 aacgaaacat tcattttaca tgatggccca ccatacgcga atggtaactt acatatggga
    17941 catgccttga acaaaatttt aaaagacttt attgtacgtt ataaaactat gcaagggttc
    18001 tatgcaccat acgtaccagg ttgggataca catggtttac caattgaaca agcattaacg
    18061 aaaaaaggtg ttgaccgaaa gaaaatgtca acagctgaat tccgtgagaa atgtaaagaa
    18121 tttgctttag aacaaattga attacagaaa aaagatttta gacgtttagg tgttcgtggt
    18181 gactttaatg atccatatat tacattaaaa cctgaatacg aagctgcaca aattcgtatt
    18241 tttggagaaa tggcagataa aggtttaatt tataaaggta aaaagccagt ttattggtct
    18301 ccttcaagtg agtcttcatt agcagaagca gaaattgaat atcacgataa acgttcagca
    18361 tcaatttacg ttgcatttga cgttaaagat gacaaaggtg tcgttgatgc agatgctaaa
    18421 tttattatct ggacaacaac gccatggaca attccatcaa atgttgcgat taccgttcat
    18481 cctgaattaa aatatggtca atacaatgta aatggcgaaa aatatattat tgcagaagcc
    18541 ttgtctgacg ctgtagcaga agcactggat tgggataaag catcaatcaa attagaaaaa
    18601 gaatacacag gtaaagaatt agagtatgtt gtagcacaac atccattctt agacagagaa
    18661 tcgttagtga ttaatggtga tcatgttact acagatgctg gtacaggttg tgtacataca
    18721 gcaccaggtc acggggaaga tgactatatt gttggtcaaa aatatgaatt gccagtaatt
    18781 agtccaatcg atgataaagg tgtatttact gaagaaggcg gccaatttga agggatgttc
    18841 tatgataaag ctaataaagc cgttactgat ttattaacag aaaaaggtgc actattaaaa
    18901 ttagacttta ttacacatag ctatccacac gactggagaa caaaaaaacc tgtaatcttc
    18961 cgtgctacac cacaatggtt tgcctcaatc agtaaagtaa gacaagatat tttagatgca
    19021 atcgaaaata caaacttcaa agtaaattgg ggtaaaacac gtatttacaa tatggttcgt
    19081 gaccgtggcg aatgggttat ttctcgtcaa cgtgtgtggg gtgtaccgtt accagtattt
    19141 tatgctgaaa atggcgaaat tatcatgacg aaagaaacag tgaatcatgt tgctgattta
    19201 tttgcagaac acggttcaaa tatttggttt gaaagagaag cgaaagactt actaccagaa
    19261 ggatttacac atccaggcag ccctaacggt acatttacta aagaaacaga cattatggac
    19321 gtttggtttg attctggttc atcacaccgt ggcgtgttgg aaacaagacc ggaattaagt
    19381 ttcccagcgg atatgtattt agaaggtagt gaccaatatc gtggttggtt caactcttct
    19441 atcacaactt cagttgctac aagaggagta tcaccttata aattcttact ttctcatggt
    19501 tttgttatgg acggtgaagg taagaaaatg agtaaatctt taggtaatgt gattgtacct
    19561 gaccaagtgg ttaaacaaaa aggtgctgat attgcgagac tttgggtaag tagtacggac
    19621 tatttagctg atgttagaat ttctgatgaa attttaaaac aaacatctga tgtttatcgt
    19681 aaaatcagaa atacattaag atttatgtta ggtaacatta acgatttcaa tcctgacaca
    19741 gatagcattc ctgaatcaga gttattagaa gtggatcgtt acttgctaaa tcgtttacgt
    19801 gaatttactg caagtacgat taacaactat gaaaactttg actacttaaa tatttatcaa
    19861 gaagttcaaa actttatcaa tgttgagtta agtaatttct atttggatta cggtaaagat
    19921 attttatata ttgaacaacg tgattctcat atccgtcgta gtatgcaaac agtgttatat
    19981 caaattttag ttgatatgac gaagttgtta gcaccaatct tagtgcatac agctgaagaa
    20041 gtttggtctc atacaccaca tgttaaagaa gaaagtgttc acttagcaga catgcctaaa
    20101 gttgtagaag tagatcaagc tttattggat aaatggcgta catttatgaa tttacgtgat
    20161 gatgtgaacc gtgcattaga aactgctcgt aatgaaaaag ttattggtaa atcattagaa
    20221 gctaaagtta cgattgctag taacgataaa tttaatgcat ctgaattctt aacttcattt
    20281 gatgcattac atcaattatt tatcgtgtca caagttaaag ttgtagataa gttagacgat
    20341 caggcaacag cttatgaaca tggtgatatt gtcatcgaac atgcagatgg tgaaaaatgt
    20401 gaaagatgtt ggaactattc agaggatctt ggtgctgttg atgaattgac gcatctatgt
    20461 ccacgatgcc aacaagttgt aaaatcactt gtataattga aattgtataa agtactcata
    20521 cagatgatat aaattaaagc tctcttcata atcatgttgt agtttttgtt gacatgatga
    20581 agagagtttt tttgtgaata aaaaaatgac caagttaccg gtcatatatg taaaaaatgt
    20641 gcgatttact aaaataaaaa ttattcagga atggtacaaa ttctctgagg catataaatg
    20701 cgttatagtt gctattctca attatgttcg cgataatttt aagtaaaagt aagcacagat
    20761 attgaatttg ataggagtta attgaatgta tcataacagt aacgcaaact ttgtcaatgg
    20821 tatcacttta aatgtgagag ataagaatga attaaagcca ttttatgagg acatattagg
    20881 attaaatatt ataaatgaga cattaacatc gatacaatat gaagtaggtc aaaataatca
    20941 tgtcattaca cttgttgaat tacaaaatgg acgtgaacct ttaatgtccg aagcgggact
    21001 gtttcatatc gcaattaaac tacctcaaat tagtgattta gctaatttac taattcattt
    21061 aagcgaatat gatattccag ttaacggagg tatacagcct gcttcgttat cattattttt
    21121 tgaagacccg gaaggaaacg gttttaaatt ttatgttgat aaagacgaag cgcaatggac
    21181 gaggcaaaat aatttagtaa aaattgatat tagaccatta aatgtaccga gattagtgag
    21241 tcatgcaaca aaattgttat ggttaggtat tccagatgac gctattatag gtgcattgca
    21301 tattaagaca attcatttat cagaggtaaa agagtactac ctcgattatt ttggattaga
    21361 gcaatcggca tatatggatg attattcaat atttttagca tcgaatggct attatcaaca
    21421 tttggccatg aatgattggg tatcagcaac gaaacgtgta gaaaattttg atacgtatgg
    21481 attagcaatt gttgactttc attatcctga aacaacacat ttaaatttac aaggtccgga
    21541 tggtatctat tatcgcttta atcatatcga agttgaagat tagtatatac tttgaatgga
    21601 cgaaccatat aatgaatcgt ttttaatgat ctttttatac aagttatgaa ggaggctggg
    21661 acattaagtt cttaggcaat gtaaaaagct gatttctatt aattatttga tagaaatcag
    21721 cttttttgat atgtatttta taatgtacag ctcgttgagc tg
//
LOCUS       DAXWFK010000026        21374 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.026, whole genome shotgun sequence.
ACCESSION   DAXWFK010000026 DAXWFK010000000
VERSION     DAXWFK010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 21374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21374
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.026"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..552)
                     /locus_tag="ACGEXS_000784"
     CDS             complement(<1..552)
                     /locus_tag="ACGEXS_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HFF8011820.1"
                     /translation="MTNQDNNHQLNHRIYHFEKIYKAIKHVIVFIFMIFIAIVAIAVI
                     AMSLYFHHLTKTSDSLSDDALIKKVRQIPGDELLDHNNKNLLYEYNHSQNSLIIGPKT
                     SSPNVIKALTSSEDTLFYKHDGILPKAILRAMIQDIFNTDQSSGGSTITQQLVKNQVL
                     TNEKTYSRKANELRLAIRLEHLLS"
     gene            934..2196
                     /gene="tyrS"
                     /locus_tag="ACGEXS_000785"
     CDS             934..2196
                     /gene="tyrS"
                     /locus_tag="ACGEXS_000785"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFF8011821.1"
                     /translation="MTNVLIEDLKWRGLIYQQTDEQGIEDLLNKEQVTLYCGADPTAD
                     SLHIGHLLPFLTLRRFQEHGHRPIVLIGGGTGMIGDPSGKSEERVLQTEEQVDKNIEG
                     ISKQMHNIFEFGTDHGAVLVNNRDWLGQISLISFLRDYGKHVGVNYMLGKDSIQSRLE
                     HGISYTEFTYTILQAIDFGHLNRELNCKIQVGGSDQWGNITSGIELMRRMYGQTDAYG
                     LTIPLVTKSDGKKFGKSESGAVWLDAEKTSPYEFYQFWINQSDEDVIKFLKYFTFLGK
                     EEIDRLEQSKNEAPHLREAQKTLAEEVTKFIHGEDALNDAIRISQALFSGDLKSLSAK
                     ELKDGFKDVPQVTLSNDTTNIVEVLIETGISPSKRQAREDVNNGAIYINGERQQDVNY
                     ALAPEDKIDGEFTIIRRGKKKYFMVNYQ"
     gene            complement(2290..3564)
                     /locus_tag="ACGEXS_000786"
     CDS             complement(2290..3564)
                     /locus_tag="ACGEXS_000786"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HFF8011822.1"
                     /translation="MSDFNHTDHSTTNHSQTPRYRRPKFPWFKTVIVALIAGIIGALL
                     VLGIGKVLNSTILNKDGSTVQTTNNKGGNQLDGQSKKFGTVHEMIKSVSPTIVGVINM
                     QKASSVDDLLKGKSSKPSEAGVGSGVIYQINNNSAYIVTNNHVIDGANEIRVQLHNKK
                     QVKAKLVGKDAVTDIAVLKIENTKGIKAIQFANSSKVQTGDSVFAMGNPLGLQFANSV
                     TSGIISASERTIDAETTGGNTKVSVLQTDAAINPGNSGGALVDINGNLVGINSMKIAA
                     TQVEGIGFAIPSNEVKVTIKQLVKHGKIDRPSIGIGLINLKDIPEEEREQLHTDREDG
                     IYVAKADSDIDLKKGDIITEIDGKKIKDDVDLRSYLYENKKPGESVTVTVIRDGKTKE
                     VKVKLKQQKEQPKRQSRSERQSPGQGDRDFFR"
     gene            3736..4353
                     /locus_tag="ACGEXS_000787"
     CDS             3736..4353
                     /locus_tag="ACGEXS_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFF8011823.1"
                     /translation="MYSVISKILNFILVKMSKSLYVIGKDNIPKDSKYVVTCTHESYN
                     EVIMLGMALYPNQIHYMAKKELFKNKWIGKFLTSLNAFPVDRENPGPSTLKRPINLLK
                     DNKTVGIFPTGSRTSQEGAPLKRGASTIAMLSKSPILPVAYVGPTKIHGLLTGQAYIN
                     IGKPIDINDLPKDLKRNERIDYITKEIETRTAKLQQELHEIVKSL"
     gene            4481..5947
                     /gene="nagE"
                     /locus_tag="ACGEXS_000788"
     CDS             4481..5947
                     /gene="nagE"
                     /locus_tag="ACGEXS_000788"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFF8011824.1"
                     /translation="MYKFFQNLGRSLMLPVAILPAAAIIAGIGNTLNALHATPKIAMF
                     FTTVGTTILEQLGILFAIGVAIGMAKKNDGAVALAATLGYFLVTVVLSPMKLAPLLGM
                     KASEINSAFEKMNNGNVFVGIVIGLIAAYAYNKFSETELPLALSFFSGKRLVPIMTAF
                     YCTFLVVILLFLWPLLYSWIVKFGESIVGLGSFGAFIYGVANRLLIPTGLHHALNSVF
                     WFDTIGINDIGKFQSGKDAIKGITGRYQAGFFPIMMFGIPAAALAMYHTAKTTQKKQV
                     YGWFLASSVAAFFVGVTEPIEFAFMFVAPILYVVHALLTGLSLFIAATFHWTAGFSFS
                     AGLIDYVLSLINPVSNHPLMLLVQGVVFFILYYVIFRVVIQVFNLNTIGRGENELVDP
                     TVVKDNIAPGENDIKQSKYHQHAIQILEGLGGQENIVNLTNCATRLRLELKDTSIIDK
                     QKIKNAGAVGVTVNGKHSTQVIVGTHVQQVADEIEKHL"
     gene            6054..7185
                     /locus_tag="ACGEXS_000789"
                     /pseudo
     CDS             6054..7185
                     /locus_tag="ACGEXS_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500341.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
     gene            complement(7146..7241)
                     /locus_tag="ACGEXS_000790"
     CDS             complement(7146..7241)
                     /locus_tag="ACGEXS_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011825.1"
                     /translation="MKPLVQSFTTSYAVKQQSNYKLSSTPLSSPK"
     gene            complement(7335..8939)
                     /gene="serA"
                     /locus_tag="ACGEXS_000791"
     CDS             complement(7335..8939)
                     /gene="serA"
                     /locus_tag="ACGEXS_000791"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFF8011826.1"
                     /translation="MKQFNVLVADPISKDGIKALLDHEQFNVDIQTGLSEEALIKIIP
                     SYHALIVRSQTTVTENIINAADSLKVIARAGVGVDNINIDAATLKGILVINAPDGNTI
                     SATEHTLAMLLSMARNIPQAHQSLTNKEWNRNAFKGTELYYKTLGVIGAGRIGLGVAK
                     RAQSFGMKILAFDPYLTDEKAKSLSITKATVDEIAQHSDFVTLHTPLTPKTKGLINAV
                     FFAKAKPSLQIINVARGGIIDEKALIKALDEGQISRAAIDVFEHEPATDSPLVAHDKI
                     IVTPHLGASTVEAQEKVAISVSNEIIEILIDGTVTHAVNAPKMDLSNIDDTVKSFINL
                     SQTVGELAIQLMYNAPSSIKITYGGDLASIDSSLLTRTIITHILKDDLGPEVNIINAL
                     MLLNQQQVTLNIENNKAETGFSNYLEVELSNDSDSVKVGASVFTGFGPRIVRINNFSV
                     DLKPNQYQIVSYHNDTPGMVGKTGALLGKYNINIASMTLGRTEAGGDALMILSVDQPV
                     SNNIIDELKQVGEYNQIFTTELTVQS"
     gene            complement(8926..10086)
                     /locus_tag="ACGEXS_000792"
     CDS             complement(8926..10086)
                     /locus_tag="ACGEXS_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HFF8011827.1"
                     /translation="MYYHQPLLLTPGPTPVPDAIRREIQAPMVGHRSKDFEDIAQQAF
                     QGLKPIFGSQNDVLILTSSGTSVLEASMLNIVNPEDHFVVIVSGAFGNRFKQIAQTYY
                     KNVHIYDVTWGEAVDVKDFINFLSTLNVEVKAVFSQYCETSTTVLHPIHELGNAINQF
                     NSNIYFVVDGVSCIGAVDVDINKDKIDVLVSGSQKAIMLPPGLAFVAYSHRAKEHFKE
                     VTTPKFYLDLNKYISSQADNSTPFTPNVSLFRGVNAYVETVKAEGFNHVIARHYAIRN
                     ALRSALKALDLTLLVNDKDASPTVTAFKPNTNDEVKIIKDELKNRFKITIAGGQGHLK
                     GQILRIGHMGKISPFDILSVVSALEIILTEHRKVNYIGKGISKYMEVIHEAI"
     gene            complement(10200..10646)
                     /locus_tag="ACGEXS_000793"
     CDS             complement(10200..10646)
                     /locus_tag="ACGEXS_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFF8011828.1"
                     /translation="MHQHDFKVQTSWQGGRNNVGNVQGDILSENISIPASLGGVGIGT
                     NPDEMLVSAASSCYIISLAATLERAKFTDISIEQQSIGTACLNNGKFSMSKIVHHPQI
                     QIPSDQIAQLEKRLPKLITIADNNCMISNAVRNNVDIKIYPIIQAK"
     gene            10726..11469
                     /locus_tag="ACGEXS_000794"
     CDS             10726..11469
                     /locus_tag="ACGEXS_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFF8011829.1"
                     /translation="MTLNKLKDELQIVSHRGLPSDFPENTMVGYREVMGLNVAMLEID
                     VHLTKDQHFVVIHDETIDRTSDGKGRIADYTLSQLKSFDFGSYKDVAFKGERIPTLDE
                     VLSLCLKYDKKLLIELKSPNLYPEIECKLLAFLEEKKVDATQVVIQSFDIECIEKLNT
                     LGSIYELGVLCSKRNYWYKKPNFSRIAQIASYVNPNYALVTRKFVDKAHHHQLQVMPY
                     TVNKLKTGEKLRQMGVDGLITDNPKLFIK"
     gene            complement(11685..12287)
                     /gene="rpsD"
                     /locus_tag="ACGEXS_000795"
     CDS             complement(11685..12287)
                     /gene="rpsD"
                     /locus_tag="ACGEXS_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019165370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFF8011830.1"
                     /translation="MARFRGSNWKKSRRLGISLSGTGKELEKRPYAPGQHGPNQRKKL
                     SEYGLQLREKQKLRYLYGMTERQFRNTFDIAGKKFGVHGENFMILLASRLDAVVYSLG
                     LARTRRQARQLVNHGHILVDGKRVDIPSYSVKPGQTISVREKSQKLNIIVESVEINNF
                     VPEYLNFDADSLTGTFVRLPERSELPAEINEQLIVEYYSR"
     gene            complement(12531..12995)
                     /locus_tag="ACGEXS_000796"
     CDS             complement(12531..12995)
                     /locus_tag="ACGEXS_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFF8011831.1"
                     /translation="MTTINPTNYTLLKKQAASLIEDEHHMIAILSNMSALLNDNLDQI
                     NWVGFYLLEQNELILGPFQGHPACVHIPIGKGVCGTAVSERRTQIVADVHQFEGHIAC
                     DANSKSEIVVPIFKDDKIIGVLDIDAPITDRFDDNDKEHLEAIVKIIEKQLA"
     gene            13132..14826
                     /gene="ezrA"
                     /locus_tag="ACGEXS_000797"
     CDS             13132..14826
                     /gene="ezrA"
                     /locus_tag="ACGEXS_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFF8011832.1"
                     /translation="MVLYIILAIIVIILIAVGVLFYLRSNKRQIIEKAIERKNEIETL
                     PFDQNLAQLSKLNLKGETKTKYDAMKKDNVESTNKYLAPVEEKIHNAEALLDKFSFNA
                     SQSEIDDANELMDSYEQSYQQQLEDVNEIIALYKDNDELYDKCKVDYREMKRDVLANR
                     HQFGEAASLLETEIEKFEPRLEQYEVLKADGNYVQAHNHIAALNEQMKQLRSYMEEIP
                     ELIRETQKELPGQFQDLKYGCRDLKVEGYDLDHVKVDSTLQSLKTELSFVEPLISRLE
                     LEEANDKLANINDKLDDMYDLIEHEVKAKNDVEETKDIITDNLFKAKDMNYTLQTEIE
                     YVRENYYINESDAQSVRQFENEIQSLISVYDDILKEMSKSAVRYSEVQDNLQYLEDHV
                     TVINDKQEKLQNHLIQLREDEAEAEDNLLRVQSKKEEVYRRLLASNLTSVPERFIIMK
                     NEIDHEVRDVNEQFSERPIHVKQLKDKVSKIVIQMNTFEDEANDVLVNAVYAEKLIQY
                     GNRYRKDYSNVDKSLNEAERLFKNNRYKRAIEIAEQALESVEPGVTKHIEEEVIKQ"
     gene            complement(14900..15040)
                     /locus_tag="ACGEXS_000798"
     CDS             complement(14900..15040)
                     /locus_tag="ACGEXS_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011833.1"
                     /translation="MIMLFSLLILIKTTHLITFIFKQLLFIFIVPFIYPYKKEARQRI
                     VG"
     gene            15189..16337
                     /locus_tag="ACGEXS_000799"
     CDS             15189..16337
                     /locus_tag="ACGEXS_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFF8011834.1"
                     /translation="MFILIYLDNAATTKAFEEVLDTYLKVNQSMYYNPNSPHKAGLQA
                     NQLLQQAKTQINAMINSKTNYDVVFTSGATESNNLALKGIAYRKFDTAKEIITSVLEH
                     PSVLEVVRYLEAHEGFKVKYVDVKKDGSINLEHFKELMSDKVGLVTCMYVNNVTGQIQ
                     PIPQMAKVIKNYPKAHFHVDAVQAFGKISMDLNNIDSISLSGHKFNGLKGQGVLLVNH
                     IQNVEPTVHGGGQEYGVRSGTVNLPNDIAMVKAMKIANENFEALNAFVTELNNDVRQF
                     LNKYHGVYINSSTSGSPFVLNISFPGVKGEVLVNAFSKYDIMISTTSACSSKRNKLNE
                     VLAAMGLSDKSIEGSIRLSFGATTTKEDIARFKEIFIIIYEEIKELLK"
     gene            16337..17560
                     /gene="thiI"
                     /locus_tag="ACGEXS_000800"
     CDS             16337..17560
                     /gene="thiI"
                     /locus_tag="ACGEXS_000800"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFF8011835.1"
                     /translation="MKYDHLLVRYGELTLKGSNRKKFVNQLRNNVNKSLKGLDGFVVK
                     GKRDRMYIELEDHADINEITYRLSKIFGIKSISPVLKVEKTIEAISAAAIKFAQQFEE
                     NSTFKIDVKRADKNFPMDTYELQRELGGAVLKHFDNISVNVKRPDHEIRVEVRLDAIY
                     MYEEVVPGSGGLPVGTGGKTLLMLSGGIDSPVAGMEVMRRGVTIEAIHFHSPPFTSDQ
                     AKEKVIELTRILAERVGPIKLHIVPFTELQKQVNKVVHPRYTMTSTRRMMMRVADKLV
                     HQIGALAIVNGENLGQVASQTLHSMYAINNVTSTPVLRPLLTYDKEEIIIKSKEIGTF
                     ETSIQPFEDCCTIFTPKNPVTEPNFDKVVQYESVFDFEEMINRAVENIETLEITSDYK
                     TIKEQQTNQLINDFL"
     gene            17604..18374
                     /locus_tag="ACGEXS_000801"
     CDS             17604..18374
                     /locus_tag="ACGEXS_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFF8011836.1"
                     /translation="MDLNLTMIIIIILFGFIAAFIDSVVGGGGLISTPALLAIGLPPS
                     VALGTNKLASSFGSLTSTIKFIRSGKVDLYVVAKLFGFVFLASACGAYIATMVPSQIL
                     KPLIIIALSSVFIFTLLKKDWGNTRTFTQFTFKKAIIFAALFILIGFYDGFVGGGTGS
                     FMLFVLLVFGFDFLSAAGNAKVLNFASNIGALVLFMVLGQVDYVIGLIMAISMIAGSY
                     AGAHFAIKQGVGYVKVLFIIITAILILKNAFDYIQQFV"
     gene            18472..18966
                     /gene="tpx"
                     /locus_tag="ACGEXS_000802"
     CDS             18472..18966
                     /gene="tpx"
                     /locus_tag="ACGEXS_000802"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFF8011837.1"
                     /translation="MTEITFKGGPIHLKGQQINEGDFAPDFTVLDNDLNQVTLADYAG
                     KKKLISVVPSIDTGVCDQQTRKFNSDASKEEGIVLTISADLPFAQKRWCASAGLDNVI
                     TLSDHRDLSFGENYGVVMEELRLLARAVFVLDADNKVVYKEIVSEGTDFPDFDAALAA
                     YKNI"
     gene            19089..20036
                     /locus_tag="ACGEXS_000803"
     CDS             19089..20036
                     /locus_tag="ACGEXS_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8011838.1"
                     /translation="MAEQQTIMERLFHTLDEKAKTLNNENGQSFIENLGLAMEQVYTN
                     ERGLLEQSTLQDRRKAFQFAYLSLMQEEKIQANHQITPDSIGLILGFLVERFMNNQEE
                     LHIVDIASGAGHLSATVKEVLPEIAVMHHLIEVDPVLSRVSVHLANFLEIPFDVYPQD
                     AIMPLPLEEADIVIGDFPVGYYPIDERSKEFKLGFEEGHSYSHYLLIEQAINALKDAG
                     YAFLVVPSNIFTGEHVKQLEKYIATETEMQAFLNLPPTLFKNEKARKSILILQKKKSG
                     ETKPVEVLLANIPDFKIPSQFQGFMTELNQWMDTNRPKK"
     gene            20124..21326
                     /locus_tag="ACGEXS_000804"
     CDS             20124..21326
                     /locus_tag="ACGEXS_000804"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFF8011839.1"
                     /translation="MSKLILAINAGSSSLKFQLIRMPEEELVTKGLVERIGLKDSIFT
                     IEVNGEKVKTVQDIKDHVEAVDIMLDAFKAHNIINDINDIDGTGHRVVHGGEKFPESV
                     AITDEVEKEIEELSELAPLHNPANLMGIRAFRKLLPNIPHVAIFDTAFHQTMPEKAYL
                     YSLPYHYYKDYGIRKYGFHGTSHKFVSQRAAEMLDKPIEDLRIISCHIGNGASIAAID
                     GGKSIDTSMGFTPLAGVTMGTRSGNIDPALIPFIMEKTGKTAEQVLEILNKESGLLGL
                     SGTSSDLRDLSEEAESGKARSQMALDVFASKIHKYIGSYAARMHGVDVIVFTAGIGEN
                     SVEIRAKVLEGLEFMGVYWDPKKNENLLRGKEGFINYPHSPVKVVVIPTDEESMIARD
                     VMTFGGLK"
BASE COUNT         7280 a         3075 c         3885 g         7134 t
ORIGIN      
        1 tgagagtagg tgttctaatc taattgctag gcgaagttca tttgcttttc tactatatgt
       61 tttttcgttg gtaagaactt gatttttaac aagttgttgt gtaattgtgc taccacctga
      121 actttgatca gtattaaaaa tatcttgtat cattgctctt aaaatcgcct ttggtaagat
      181 gccatcatgt ttataaaata aagtgtcttc agatgacgtt aatgctttaa tgacatttgg
      241 acttgatgtt ttagggccta taatgagtga gttttgagaa tggttatact catataataa
      301 atttttgtta ttatgatcta ataattcatc gccaggtatt tgtcgaactt tttttattaa
      361 agcatcatct gataatgagt cggacgtttt agttaaatga tgaaaatata aagacatcgc
      421 aatcacagcg atagcaacga tggcaatgaa aatcataaat ataaaaacaa tgacatgttt
      481 gatagcttta tatatctttt caaaatgata tatacgatga ttcaattgat gattgttgtc
      541 ttgattcgtc atctttaacc tcctaaatta cgtatagtat accatattta agcaatttga
      601 cttttatttt aacgttaatt ataattaggg aaaatgcata aagaatagga taaaaggaca
      661 agtaatatta tttgaaatca tcagagagct agtggtcgct gtgaactagt gtttttaata
      721 atatgaatac acctttataa ctaacatgtc ctacgtttat taatgaacgt atcattaata
      781 gagtgaacat tatcttatga ttatgtttaa gtttggtggt aacgtgcaat cgcgccctta
      841 catatttgtt gtaagggttt tttttacgtt caaaaaactt taaagtattt taacaagtat
      901 tcaataaata attataaata ggaggaaata ataatgacga atgtattaat tgaagattta
      961 aaatggagag gtcttattta tcaacaaact gatgaacaag gtattgaaga tttattaaat
     1021 aaagaacaag tgacgttata ctgcggtgcc gatccaacgg cagatagttt acatattggt
     1081 cacttactac cattcttaac attaagacgt tttcaagaac atggacatcg tcctatcgtt
     1141 ttaattggcg gtggtacagg tatgattggt gatccatcag gtaaatcaga agaacgtgtg
     1201 ctacaaacag aagaacaagt agataaaaat atcgaaggta ttagtaagca aatgcacaat
     1261 atttttgaat ttggaacaga ccatggtgca gtgcttgtta ataatagaga ctggttagga
     1321 caaatctcat taattagttt tttacgtgac tatggtaaac acgtcggcgt taattacatg
     1381 ttaggtaaag attcaatcca aagtcgttta gaacatggta tttcatatac agaattcaca
     1441 tacacgattt tacaagctat tgatttcggt catttgaata gagaattgaa ttgtaagatt
     1501 caagtaggtg gatcagatca atggggtaat atcacaagtg gtattgaatt aatgcgtcgt
     1561 atgtatggtc aaacagacgc atacggttta actattccgc ttgtaactaa atcagatggt
     1621 aagaaatttg gtaagtctga gtcaggtgct gtttggttag atgctgaaaa aacaagtcct
     1681 tatgaatttt atcaattctg gattaatcaa tcagacgaag atgtaattaa attcttaaaa
     1741 tactttactt tcttaggaaa agaagaaatt gatcgcttag aacaatctaa aaatgaagca
     1801 ccgcatttac gtgaagctca aaaaacatta gctgaagaag taactaaatt tattcatggt
     1861 gaagatgcat taaatgatgc aatccgtatt tcacaagcat tatttagtgg tgatttaaaa
     1921 tcattatcag cgaaagaatt aaaagatgga tttaaagatg tgcctcaagt gacattatca
     1981 aatgacacaa caaatatcgt tgaagtcctt attgaaacag gcatttctcc ttctaaacga
     2041 caagcacgtg aagatgttaa caatggtgcg atttatatta atggtgagag acaacaagat
     2101 gttaattatg ctttagcacc agaagataaa attgatggcg aatttacgat tattcgtcgc
     2161 ggtaagaaaa aatacttcat ggttaactat caataaatat aattgcatag ctaaataaat
     2221 tagagcctac tcatattcat tcctaagaat gtaatgagta ggctcttaat gtacttttct
     2281 gtctgtaaat tatctaaaga aatctctatc gccttggcca ggtgattgac gttctgatcg
     2341 gctttgacgt tttggttgtt ctttttgttg ttttaatttc actttaactt cttttgtttt
     2401 accatcacgg ataacggtaa cagtgactga ttcaccaggt tttttatttt catataaata
     2461 gcttcttaaa tcaacatcat ctttaatttt cttgccatca atttctgtaa taatatcacc
     2521 ttttttaaga tcaatatcac tatcagcttt ggcgacataa ataccgtctt ctctatcagt
     2581 atgaagttgc tcgcgctctt cttcaggaat atctttcaaa ttaattaaac caataccaat
     2641 cgaagggcgg tcaattttac catgttttac aagttgttta attgttactt taacttcatt
     2701 acttggaata gcaaacccga taccttcaac ttgtgtcgca gcaattttca ttgagttaat
     2761 accaactaaa ttaccattaa tatctactaa tgcgccacct gagttacctg ggttaatagc
     2821 agcatctgtt tgaagaacgc taacttttgt attgccacca gttgtctcag cgtcaatcgt
     2881 acgttcgctt gctgaaatga taccagatgt tacagagtta gcaaattgta atcctaatgg
     2941 gttacccatt gcgaatacgc tatcgccagt ttgtactttt gaagagttgg caaattgaat
     3001 cgctttaata ccttttgtat tttcaatttt aagtacagca atatcagtta ctgcatcttt
     3061 accaactaat ttcgctttaa cttgtttttt attatgtaat tggactctaa tttcatttgc
     3121 gccatcaata acatgattgt ttgtaacgat ataagctgaa ttgttgttta tttgatagat
     3181 aacacctgaa cctactccag cttcagatgg tttagatgat ttgcctttta ataagtcgtc
     3241 tacacttgat gctttttgca tgttaataac tccaacaatt gtaggggaga cagattttat
     3301 catttcatga acggtaccga atttcttgct ttgaccgtct aattgattgc cacctttatt
     3361 atttgttgtc tgaacagttg aaccatcttt atttaaaatt gtactattta atactttgcc
     3421 tataccaagt actagaagtg caccaataat tccagcaatc aatgcaacga tgactgtttt
     3481 aaaccatgga aatttaggtc ttctgtatct aggtgtttgg ctatggtttg ttgtagaatg
     3541 atctgtatga ttaaaatctg acatacttaa cctccattat atgatttata tatgctttaa
     3601 ttatgtcttt tttttataat tattgctagt aatacactat acaactttag atgtattttc
     3661 agactatctt gttctagtaa aaatatagtg ataaaatcta gttgttataa acaaataagt
     3721 taagtaggtg gcacaatgta ttcagtgatt agtaagattt tgaatttcat tttagttaaa
     3781 atgtcaaaat cattatacgt aatcggtaaa gataatattc ctaaggatag taaatatgtc
     3841 gtaacttgta cgcatgaaag ttataacgaa gtcattatgc taggtatggc attatatcct
     3901 aatcaaattc attatatggc taaaaaagaa ttattcaaaa ataaatggat tggtaaattt
     3961 ttaacgtctt taaatgcatt tcctgtagat cgtgaaaatc caggacctag tactttaaaa
     4021 cgcccaatca atttgttgaa agataataag acagttggta tcttcccaac aggttctcgc
     4081 acatcgcaag aaggtgcacc tttgaaacgt ggtgcttcaa caattgcaat gttaagtaag
     4141 tcaccaatat tacctgttgc ttatgttggt cctactaaga ttcacggtct tttaacagga
     4201 caagcataca taaatattgg caaaccaatc gatattaacg atttacctaa agatttaaaa
     4261 cgaaacgagc gtatcgatta tattacaaaa gagattgaaa cacgaacagc aaagttacaa
     4321 caagaattgc atgaaattgt aaaaagttta taaaataact tgaggtagaa atatagctgg
     4381 ttagtaagcc taggtgacta taacggagta tcgaagtagt tattctagtt acattagtaa
     4441 tctttatatt tctacattat tttaagaagg atgacattca atgtataaat ttttccaaaa
     4501 tttagggcgt tcgttaatgt taccagttgc cattttaccg gctgcagcaa ttattgccgg
     4561 aattggtaat acgttaaatg cacttcatgc gacgccgaaa atcgcaatgt tctttactac
     4621 ggtaggtact acgattttag agcaattagg tattttattt gcaattggtg tagctatagg
     4681 aatggcaaag aaaaatgatg gtgcagtcgc gcttgcagct acgttaggat attttttagt
     4741 tacagttgta ttatcaccaa tgaaattagc gcctttatta ggaatgaaag cttcggaaat
     4801 aaattcagct tttgaaaaga tgaataatgg caatgtcttt gttggtattg taataggttt
     4861 aatagcagct tatgcataca ataagtttag tgaaacagaa ttaccattag cattatcatt
     4921 ttttagtggt aaacgtctag ttccaattat gactgcattt tactgtacat ttttagttgt
     4981 catattgtta ttcttatggc cactacttta ttcatggatt gtaaaatttg gtgaatctat
     5041 agtcggttta ggatcgtttg gagctttcat atatggtgtt gccaatagat tattaattcc
     5101 tacaggatta catcatgcac tgaatagtgt gttctggttt gatacgattg gtattaatga
     5161 tattggtaaa ttccaaagtg gcaaagatgc tataaaaggt attacaggac gctatcaagc
     5221 gggattcttc ccaatcatga tgttcggtat tccagcggct gcgttagcaa tgtatcatac
     5281 agctaaaact actcagaaaa aacaagttta cggttggttc ctagcgagtt cagttgcagc
     5341 atttttcgtt ggtgttacag agccgattga gtttgcattt atgtttgttg cgccaatctt
     5401 atatgttgtt catgcgttat taacaggatt atctttattt atcgcagcta cattccattg
     5461 gacagctgga ttttcattta gtgcaggttt gatagattat gtgttatcac taattaatcc
     5521 agtatcaaat catccattga tgttgcttgt tcaaggtgtt gtatttttca tcttatacta
     5581 tgtgattttc cgtgtagtaa tccaagtatt taacttgaat acgattggta gaggtgaaaa
     5641 tgaattagtt gacccaacag ttgtaaaaga taatattgct cctggtgaaa atgatattaa
     5701 acaaagtaaa tatcatcaac atgctataca aatattagaa ggtttaggcg gtcaagagaa
     5761 tattgttaat ttaaccaatt gtgcaacaag gttgcgtcta gagttaaaag acacatctat
     5821 tattgataaa cagaagatta aaaacgccgg tgcagttggt gttactgtta atggcaaaca
     5881 ttcaactcaa gttattgtcg gtacacacgt acagcaggtt gctgatgaaa ttgaaaaaca
     5941 tttataaaat ttaaacagct aaagcgcaaa tctaacttca atagggtttg cgcttttttt
     6001 agtcaaatag tagaatgatt tatatctgaa ttttcagaaa tggagtgggg attgtgaaga
     6061 aaattttatt tgatgttgat ggcgtttttt ttaagtgaag aacgttgttt cgatgtctct
     6121 gcattaacgg tatatgaatt attaatggat aagtgttatt taggtcttca ttcacatata
     6181 gattgggaaa ctttgactga taacgatata caagacatta ggaatcgaat ttttcaaaaa
     6241 gataagattt taaacaaatt aaaatcgcta gggctgaatt caaattggga tatgttattt
     6301 atcgttttta gtattcattt aattgatatt ttgaaaaagt tatcacatga tgaaattgag
     6361 gcattcatgt atcaagatga gcctgtcgaa ttgaagttgc aaaatataag cacaaactta
     6421 gcagactgtt ttaatttaaa tgaacaacta cctttacaat ttttggataa tgtaaaagtt
     6481 ggtaaaaata atatttatgc tgctttggaa gagtttgcaa caacagagtt acatgtttcg
     6541 gatgctactt tatttagttt gaagggtgca ttatggacgt tagcgcaaga agtttatcaa
     6601 gaatggtatt taggatcgaa gttgtatgaa gatgttgaaa agaaaatagc acgaactact
     6661 tttaagacag gttatattta tcaagaaatt attttgagac cagtagatga agttaaggta
     6721 cttctgaatg atttaaaagg tgctggtttc gaattaggta ttgcaacagg tcgtccttat
     6781 actgagactg ttgtgccatt tgaaaattta ggattgttac catattttga agctgatttt
     6841 attgcaacag caagtgatgt tttagaagca gagaatatgt atccgcaagc acgaccatta
     6901 ggaaagccga atccttttag ttatatcgca gctttatatg gtaataatcg cgataaatat
     6961 gaatcttata tcaataagca agataacatt gtaaataaag atgacgtatt tatagtaggc
     7021 gattcgttag ctgacttatt aagtgctcaa aaaataggtg caacgtttat tggaacatta
     7081 acaggtttaa aaggtaagga tgctgcaggt gagttagaag cgcatcatgc cgactatgtt
     7141 attaatcatt taggtgaact tagaggtgta ctagataatt tgtaatttga ttgttgtttg
     7201 acagcataac ttgtagtgaa tgattgaacc aaaggtttca tattgagtta caatgaaatt
     7261 aataatgaaa aaatgccaag aagcaatgga agtaatccaa tgtcttcttg gcattttgaa
     7321 tttacataaa ttgtttatga ctgtaccgtc aattcagttg tgaaaatttg attgtattca
     7381 ccaacttgtt taagttcatc aattatattg tttgaaacag gttgatcaac ggataaaatc
     7441 attagcgcat ctccgcccgc ttcagttcta cctaaagtca tagatgcaat gttgatattg
     7501 tatttaccta acaatgcgcc agtttttcct accatacctg gagtatcatt atgatatgac
     7561 acaatttgat attgatttgg ctttaagtct acagaaaaat tattaattct aacaattctt
     7621 ggaccgaaac ctgtaaagac agaagcgcca actttaacgg aatcgctatc gtttgatagt
     7681 tctacctcta agtagttact aaaacctgtc tctgctttat tattttcaat atttaatgtc
     7741 acttgttgtt gatttaacaa cattagagca ttgataatat tgacttcagg accaagatca
     7801 tcttttaaaa tatgagtaat aattgtacgt gttaataaac tactatcaat agaggctaag
     7861 tcgccaccgt acgtaatttt aatagagctt ggtgcattgt acattaattg aatagctaat
     7921 tcaccaactg tttggcttaa attgatgaat gattttacag tatcatctat attgcttaag
     7981 tccattttag gtgcattcac tgcatgcgtt acagtaccat caattaaaat ttcgatgatt
     8041 tcatttgaaa cagaaattgc cactttttct tgagcttcga ctgttgaagc acccaaatga
     8101 ggtgtaacaa taattttatc atgtgcaaca agaggcgagt cagttgcagg ttcatgttca
     8161 aacacatcga tagctgcccg actaatttgt ccttcgtcta atgcttttat tagcgccttt
     8221 tcatcaataa taccaccacg tgccacattg attatttgca aactaggttt tgctttggca
     8281 aaaaagacag cattaattaa gccttttgtt ttaggtgtta gtggtgtatg taatgtaacg
     8341 aaatcagaat gttgggcaat ctcatcaact gttgccttcg taatgcttaa agattttgct
     8401 ttttcatccg ttaagtaagg gtcaaaagct agtattttca ttccgaaact ttgcgcacgt
     8461 ttagcaacac ctaaaccaat tctaccagca ccaatgacac ctaatgtttt ataataaagc
     8521 tcagtacctt taaatgcatt tcgattccat tctttatttg taagtgattg gtgtgcttgc
     8581 ggaatatttc gtgccattga taataacatt gccagtgtat gttcagtagc tgaaatcgta
     8641 ttaccatctg gggcattaat aactaaaata ccttttaatg ttgcagcatc aatattaata
     8701 ttatctacac caacaccggc gcgtgcgatt acttttaaag aatcagcagc atttatgata
     8761 ttttcagtaa ccgtagtttg actacgaacg attaaagcat ggtatgaagg tataattttg
     8821 attaatgctt cttcggacaa gccagtttga atatctacat tgaattgttc gtgatctaat
     8881 aatgctttga taccatcttt tgatatggga tctgcaacga gtacattaaa ttgcttcatg
     8941 aataacctcc atatattttg atataccttt accgatatag ttaactttac ggtgttcagt
     9001 taaaataatt tctaaagcag atactaccga taaaatatcg aaaggactaa ttttccccat
     9061 atgaccaatt cttaaaattt gacctttaag atggccttga ccaccagcaa ttgttatttt
     9121 aaagcgattt ttaagttcat ctttgattat tttcacttca tcatttgtat taggtttgaa
     9181 tgctgtaacc gttggagatg catctttatc attgactaat aaagttaaat ctaatgcttt
     9241 taaggcgctt cttaatgcat ttctaattgc atagtgtcgt gctattacgt gattgaaacc
     9301 ttctgctttt acggtttcaa cgtatgcatt tacacctcta aataaagaca catttggtgt
     9361 gaacggtgta gaattgtcag cttgtgacga aatgtattta tttaagtcta gataaaattt
     9421 tggcgtagtt acttctttga aatgttcttt tgcacggtgg ctataagcta caaaagctaa
     9481 tcctggaggt aacataattg ctttttgact accagaaaca agtacatcaa ttttatcttt
     9541 gttaatgtca acatcaacag caccaatgca acttacgccg tcaactacaa aataaatatt
     9601 actattaaat tgattaatgg catttcctaa ctcgtgaata gggtgtagca ctgtcgtaga
     9661 tgtttcgcaa tattgactaa atactgcttt aacttcaaca tttaaagttg aaaggaaatt
     9721 gatgaaatct ttgacatcta cagcttctcc ccatgttacg tcataaatat gcacattttt
     9781 gtaataagtt tgtgcaattt gtttaaatcg gttaccaaag gcacctgaaa caatgacaac
     9841 gaagtgatct tcagggttta caatgttcaa catactagcc tccaagacgc ttgtaccgct
     9901 agatgttaaa ataagtacat cattttgact cccaaatatt ggctttagac cttgaaatgc
     9961 ttgttgtgcg atgtcttcaa aatctttaga acgatgacca accataggtg cttgaatttc
    10021 tctcctaatg gcatcaggta caggggttgg gccaggtgtt aataacaacg gttgatgata
    10081 atacatttat atgcctccta taaataagat ttcactattt tagcaaattt tctgaaaatt
    10141 taaaagccta aaaaagtgat aacgctatat tgtcgaacaa acgttatcac ttaacgattt
    10201 tatttggcct gaatgatggg atagattttt atgtccacat tatttcttac agcatttgaa
    10261 atcatgcaat tattatctgc aattgttatc aattttggta atcgcttttc taattgtgct
    10321 atttgatcac ttggaatttg aatttgagga tggtgcacaa tttttgacat actgaatttt
    10381 ccgttattta aacaagctgt tccaatcgat tgttgttcaa ttgaaatatc tgtgaacttt
    10441 gcacgttcaa gagtagctgc taatgagatg atataacatg atgaagcggc tgatactaac
    10501 atttcatcgg gatttgttcc tataccaaca ccacctaaag aagcaggtat agaaatattc
    10561 tctgaaagta tgtcgccttg aacgtttccg acattgttac gaccaccttg ccaagaagtt
    10621 tggactttaa agtcatgttg atgcaattgc tttaacctcc aatataattg tgatagttta
    10681 attttagaat agtttatcaa agtttaacag aaaggtgact tatcaatgac tctgaataaa
    10741 ctgaaagatg aattacaaat tgtttcgcac cgtggattgc cgagtgattt tcctgaaaat
    10801 acaatggtcg gttatcgaga ggtaatgggg ctcaatgttg ctatgttaga aatagatgtt
    10861 catttgacca aagaccaaca ttttgttgtg atacatgatg aaacaattga tagaacatcg
    10921 gatggtaagg ggcgtattgc tgattacaca ttatcgcaat taaaatcatt tgattttggt
    10981 agttataaag atgttgcttt taagggagaa cgaataccca cgttagatga agtactttca
    11041 ttatgcttaa agtatgataa aaagctattg attgaattga aaagtccgaa tttatatcca
    11101 gaaatagaat gtaaattgct ggcatttttg gaagaaaaga aagtcgatgc gacacaagta
    11161 gttatacaat cttttgatat cgaatgcatc gaaaagttaa acacattggg cagtatatat
    11221 gaattaggtg tgctttgtag taaacgtaat tattggtata aaaagccaaa cttttcaaga
    11281 attgctcaaa ttgctagtta tgttaatcct aattatgcat tagtgaccag aaaatttgtt
    11341 gataaagcac atcatcatca attacaggtt atgccataca cagttaataa attgaaaact
    11401 ggagaaaaat taagacaaat gggtgtagat ggattaatta cagataatcc gaaattattt
    11461 attaagtgag acgatgaatg gtaatttgat agagggatga ggattcgtat atcgcaattg
    11521 ataattttaa aaactaatga tgaaatacta aagataatta aacaaataag acttgtaaat
    11581 tctaagttga ttgtaaaata gggtagctcc atataatttt gaatctatca tttttaaatt
    11641 aataaaaaaa acacccacaa ttgtgggtgt ttgtattgaa cgtattatct tgagtagtac
    11701 tcaacgatta attgttcgtt aatttcagca ggtaattcgc tacgttctgg taaacgtacg
    11761 aaagtaccag ttaagctgtc agcatcaaag tttaagtact caggtacgaa attgttgatt
    11821 tcaactgatt caacgatgat gtttaatttt tgagattttt cacgaactga aattgtttga
    11881 ccaggtttaa cagaataaga tggaatatca acacgtttac catctactaa gatatgaccg
    11941 tggttaacta attgacgtgc ttgacgacga gtacgagcta aacctaatga ataaacaaca
    12001 gcgtctaaac gacttgctaa taagatcatg aagttttcac cgtgtacacc gaatttttta
    12061 ccagcgatgt caaatgtgtt acggaattgt ctttcagtca ttccatataa gtaacgtaat
    12121 ttttgttttt cacgtaattg taaaccatat tctgataatt ttttacgttg gtttggacca
    12181 tgttgtcctg gtgcgtaagg acgtttttct aattctttac cagtaccgct taaagagata
    12241 cctaaacgac gagatttttt ccagtttgaa cctctgaatc gagccataat aagactcctc
    12301 ctttaattct tttttgttgt tatgaataaa caaaaaagag tgttgcatgc ttactaagat
    12361 atattgtttt atgtgtcttc accttatagc gacagttacg tgacacgtcc gcattgggaa
    12421 caacatagcg ctcaataata tacaactgct actttcgtta attcacacaa agattattgt
    12481 aacattttcc tgctaactgt caacacttta ttgaaaatgc tgatgtcctt ttatgcgagt
    12541 tgcttttcaa taattttaac aattgcttca agatgttctt tgtcattgtc atcaaatcga
    12601 tccgttatag gggcatcgat atctaagacg ccgataattt tatcatcttt gaaaattgga
    12661 acgacaatct cagacttact attagcatca caagcgatat gtccttcgaa ttgatgaaca
    12721 tcagctacaa tttgtgtacg acgttctgaa acggctgtac cacatacacc ttttccaatt
    12781 ggaatgtgga cacaagcggg gtgtccttgg aaaggtccaa gtataagttc attttgttcc
    12841 aataagtaaa agccaaccca attaatttga tctagattat catttaataa ggcagacatg
    12901 ttacttaaaa tagcaatcat gtgatgttca tcttcaataa ggcttgctgc ttgtttcttt
    12961 aataatgtgt agtttgttgg gttaattgtt gtcatattta aaacctctta ttttcataga
    13021 ttttataata gtatagaatt tcgaaaatat tatggcaata aattcatttt ataatttatc
    13081 cttaagcaaa attacgttat aatagtaatg ataaattagg aggagaagca tatggtgtta
    13141 tatatcattt tggcaataat tgtgattata ttgattgctg taggtgtatt attctattta
    13201 cgttcaaata aacgacaaat tattgaaaaa gcaatcgaac gtaaaaatga aattgagacg
    13261 ttaccttttg atcaaaacct tgcacaatta tctaagttga atttaaaagg tgaaacaaaa
    13321 acgaaatacg atgcaatgaa aaaggacaac gtagaaagta caaataagta tctagctcct
    13381 gtggaagaaa aaatccataa tgctgaggct ttattagata aatttagttt caacgcatct
    13441 caaagtgaaa ttgatgatgc aaatgagttg atggatagtt acgaacaaag ctatcagcaa
    13501 caattagaag atgtaaatga aattattgcg ttatacaaag ataatgatga attatatgac
    13561 aaatgtaagg ttgattatcg tgaaatgaaa cgtgatgttt tagcaaatcg tcatcaattt
    13621 ggtgaggcag caagtctact tgaaactgaa attgaaaaat ttgagccaag gttagagcaa
    13681 tatgaagtac taaaagctga tggtaattat gtacaagcgc acaaccatat agctgccttg
    13741 aatgaacaaa tgaaacagct aagatcttat atggaagaaa taccagaatt aattagagaa
    13801 actcaaaaag aattacctgg tcaattccaa gatttaaaat atggttgccg tgatcttaaa
    13861 gttgaagggt atgatctgga tcacgtgaaa gtagacagta cattacaaag cttaaaaaca
    13921 gagcttagtt tcgttgaacc attaattagc cgcttagaat tagaagaagc taatgataaa
    13981 ctagctaata tcaatgataa gttagatgac atgtatgatt taattgaaca tgaagttaaa
    14041 gctaaaaatg atgtcgaaga aacaaaagat atcattacgg ataacttatt caaagctaaa
    14101 gacatgaatt atacattgca aacagaaatt gaatatgtac gtgaaaacta ctatataaat
    14161 gaatctgatg ctcagagtgt tcgtcaattt gaaaatgaaa ttcaaagttt aatttctgta
    14221 tatgatgata ttttaaaaga aatgtctaaa tctgctgtgc gatatagcga ggttcaggat
    14281 aatttacaat atttagaaga tcatgtcaca gttattaatg acaaacaaga aaagctacaa
    14341 aatcatctga ttcaattgcg tgaagatgaa gcagaagcag aagacaatct gctacgagta
    14401 caatcgaaga aagaagaagt gtatcgtcga ttacttgctt ctaacttaac aagcgttcct
    14461 gaaaggttta tcatcatgaa aaatgaaatt gatcatgaag ttcgtgatgt taacgaacaa
    14521 tttagtgaac gtccaataca cgttaaacag ttaaaagata aagtgtctaa aattgtgatt
    14581 caaatgaata catttgaaga tgaagcaaat gatgttcttg ttaatgctgt ttatgcagag
    14641 aaattaattc aatatggaaa tagatatcgt aaggactata gcaatgttga taagagctta
    14701 aatgaagctg aacgattatt taaaaataat cgctataagc gtgcgattga aattgcagag
    14761 caagctcttg aaagtgttga gccaggtgtt actaaacata ttgaagaaga agttattaag
    14821 caatagaaac tagtatgtag ttatacttaa ataatatgag cactctgtca aattggactg
    14881 atgagtttaa taattgaagt tagccaacga tacgttgtct agcttctttt ttatatggat
    14941 aaatgaaagg gacaataaat ataaatagca attgtttaaa gataaacgta atcaaatgtg
    15001 ttgttttaat taatataagt agtgaaaaaa gcataatcac acagctgttt aaatagagtg
    15061 aaatagtcta attcttattt aataagtaga aataagatta ttttgcgcta taacctttat
    15121 tttttgtcgg ataatagtat aatgcttgag taaaacaatt ttaacgattg atgattaatg
    15181 aaagagaagt gtttattttg atatatctag ataatgcggc aacgacgaaa gcatttgaag
    15241 aagtgttaga tacttattta aaagtaaatc aatcaatgta ttataatccg aatagtccgc
    15301 ataaagctgg tttgcaggca aatcaattac tacaacaagc aaaaacccaa attaatgcaa
    15361 tgattaattc aaaaacaaat tatgatgttg tattcactag tggtgcaact gaatccaata
    15421 atcttgcttt aaaaggtatt gcctatcgta aatttgatac agcgaaggaa ataattacat
    15481 ccgtgttaga gcatccgtcc gtattagagg ttgtaagata tttggaagca cacgaagggt
    15541 ttaaagttaa atatgttgat gtaaagaaag atggcagtat taacttagaa cacttcaaag
    15601 aattaatgtc agacaaagtc ggtttagtaa catgtatgta tgtaaataat gtaactggac
    15661 aaatacagcc tattccacaa atggctaaag ttataaaaaa ttatcctaag gcacattttc
    15721 atgtagatgc ggttcaagca ttcggcaaaa tttcaatgga tctcaataac atagatagta
    15781 ttagtttaag tggacacaag tttaatggtt taaaaggaca aggcgtctta cttgtaaatc
    15841 acattcaaaa tgttgaacca actgtccatg gtggtggtca agaatatggc gttagaagtg
    15901 gaacagttaa tttgccaaat gatattgcaa tggttaaagc gatgaagata gctaatgaaa
    15961 actttgaagc attgaatgca tttgttactg agttaaataa tgacgtccgt caatttttaa
    16021 ataaatatca tggagtttat attaattctt caacttcagg ttcaccattc gttttaaata
    16081 ttagttttcc tggcgtaaaa ggtgaagtat tagttaatgc tttttcaaaa tatgacatta
    16141 tgatatctac gacaagtgct tgttcatcta aacgtaataa attaaatgaa gtattggctg
    16201 caatgggatt atcagacaaa tctattgaag gtagtataag attatcattt ggggctacta
    16261 caactaaaga agatatagcg aggtttaaag aaatatttat catcatttat gaggaaatta
    16321 aggagttgct aaaataatga agtatgatca cttgcttgtt agatacgggg agttaacatt
    16381 aaagggttca aatagaaaga aatttgtaaa tcaattaaga aataatgtaa ataagtcatt
    16441 aaaaggactt gatgggtttg tcgttaaagg caaacgagat cgtatgtata ttgaacttga
    16501 agaccatgca gatataaatg aaataacata tcgattatca aaaattttcg gtattaaatc
    16561 tattagtcca gtattaaaag tagaaaaaac aatagaggca ataagtgcag cggcaattaa
    16621 atttgcgcag caatttgaag aaaacagcac atttaaaatt gatgtgaagc gtgccgataa
    16681 aaatttccca atggatacgt atgaattaca gcgtgaattg ggtggtgcag tattgaagca
    16741 cttcgacaat atttcagtga atgtcaaacg tccagatcat gaaattcgag tggaagttag
    16801 attagatgca atttatatgt atgaagaagt tgttccgggt tcaggtggat taccagttgg
    16861 tactggtggt aagacgttac taatgctttc aggcggtata gactcaccag ttgctgggat
    16921 ggaagtgatg agacgtggcg taacaattga agcgattcat ttccatagtc caccatttac
    16981 aagtgatcaa gcaaaagaaa aagttattga attgacacgt attttagctg aacgtgttgg
    17041 accaattaaa ttgcatattg taccatttac agaattgcaa aaacaggtaa ataaagttgt
    17101 acatccaaga tatacaatga cttcaacgag acgtatgatg atgcgtgttg ctgataaatt
    17161 agtacatcaa ataggggctt tagctattgt aaatggtgaa aacctagggc aggtagccag
    17221 tcaaacactt catagcatgt atgcaattaa taatgtaact tctactcctg tattacgtcc
    17281 tttattaact tacgataaag aagaaattat tattaaatcg aaagaaattg gtacatttga
    17341 aacatctatt caaccatttg aagattgttg tacaattttc acccctaaaa atccagtaac
    17401 cgaaccaaac tttgataagg tagtccaata tgaaagtgtc tttgattttg aagagatgat
    17461 taatcgtgct gttgaaaata ttgaaacact tgaaataact agtgattata aaactattaa
    17521 agaacagcaa acaaaccaat taataaacga ctttttataa ataaaatcct agagtaaatt
    17581 taaacataag gggatgttaa actatggatt tgaacttaac gatgattata atcataattt
    17641 tatttggttt tatcgcggcg tttatagatt cggttgtagg gggtggcggt ttaatttcta
    17701 cgccagcatt attagcaatc ggtctaccac catctgtggc tttaggtaca aataaattgg
    17761 caagttcgtt tggttcttta actagtacga taaagtttat aaggtccggt aaagtggact
    17821 tatatgttgt tgccaaatta tttggttttg tatttttggc atctgcatgt ggcgcatata
    17881 ttgcaacgat ggttccgtca caaatattga aacctttaat catcattgca ctttcgtcgg
    17941 tgtttatatt cacattactt aaaaaagatt ggggcaatac acgcacgttt actcaattta
    18001 catttaagaa agccataata tttgcagcac tttttatatt aatcggcttt tatgatggat
    18061 ttgtaggtgg gggaacaggt tcatttatgc tttttgtatt gttagtcttt ggttttgatt
    18121 ttttaagtgc agcaggaaat gctaaagttt tgaactttgc ttctaatata ggtgcgcttg
    18181 tattatttat ggtattagga caagtagatt atgtaatagg tttaattatg gctataagta
    18241 tgattgctgg gtcatatgct ggggctcatt ttgctattaa acaaggtgtt ggttatgtaa
    18301 aagtattatt tattattatt actgctattt taattttaaa aaatgctttt gattatattc
    18361 aacaatttgt ataaaattaa atttgctttt gattaaagca tgaaaattgt aatcaaacca
    18421 taaattgtcg tatgatgtag ttagaatttt aaaatgcagg aggtcaagta tatgactgaa
    18481 ataacattca aaggtggacc aatccactta aaaggtcaac aaattaatga aggtgatttt
    18541 gcacctgatt ttacagtgtt agataatgac ttaaatcaag taacattagc agattatgct
    18601 ggtaaaaaga aattaattag tgtggtacca tcaattgata caggtgtttg tgatcagcag
    18661 actcgcaaat tcaactctga tgcttctaaa gaagagggga ttgtgcttac aatttcagca
    18721 gacttaccat tcgcacaaaa aagatggtgc gcttcagcag gtttagacaa tgtcattaca
    18781 ttaagtgacc accgtgactt atcatttggt gaaaactatg gcgttgttat ggaagaactt
    18841 cgcttattag ctcgtgcagt atttgtatta gatgcagata ataaagttgt ttataaagaa
    18901 atcgttagtg aaggtactga tttcccagat tttgatgctg ctttagctgc atacaaaaat
    18961 atttaatcat taaagagata aatcttaaaa tgtatacatc gtgtccatcg ttgtcaacag
    19021 cattaaaata gaattgtttt ctatgattgc taagacctat gggcactttt tattggagag
    19081 ggacgaatat ggcagaacaa caaacaatta tggaacgctt gtttcataca ttagatgaaa
    19141 aagctaaaac attaaataat gaaaatggcc aaagttttat tgaaaatctt gggctagcaa
    19201 tggaacaagt atataccaat gaaagaggat tgttagaaca atcgacgtta caagatcgac
    19261 gtaaagcatt ccaatttgca tatttaagtt taatgcagga agaaaagata caagcaaatc
    19321 atcaaattac accagattca attggattga tactaggatt tttagttgag cgttttatga
    19381 acaaccaaga agaattacat attgttgata ttgcaagtgg tgccggtcat ttaagtgcta
    19441 ctgtaaaaga agtgttacct gaaattgcgg ttatgcatca tttaattgaa gttgatccag
    19501 ttttatcacg tgttagtgta catttagcaa acttcttaga aattcctttc gatgtgtatc
    19561 ctcaagatgc catcatgcca ctaccattag aagaagcaga tatcgttatt ggtgattttc
    19621 cagtaggcta ttatccaatt gatgaaagaa gtaaggagtt taagctaggt tttgaagaag
    19681 gacatagtta ttcacattat ttattaatag aacaagcaat aaatgcatta aaagatgctg
    19741 gatatgcctt tctagtggta ccaagtaata tttttacagg tgaacatgta aaacagcttg
    19801 aaaaatatat tgcaacagag acagagatgc aagcattttt aaatttacca ccaactttat
    19861 ttaaaaatga aaaagcgcga aaatctatat taattttaca aaagaaaaaa tcgggtgaaa
    19921 caaagccagt tgaagtatta ttggcgaata ttcctgattt caaaattcct tcacaatttc
    19981 aaggatttat gacagagtta aatcagtgga tggacacaaa tcgtcctaaa aaataatctg
    20041 taatagtata gtcataaaac tgtatgaatg ataaaatgaa aaatgaaata gtataaaaag
    20101 caaacgatgg aggatcatta cttatgtcaa aattaatctt ggctatcaat gctggtagtt
    20161 catcattaaa atttcaatta attagaatgc ctgaagagga attagtaaca aaaggtttag
    20221 tagaaagaat cggattgaag gattcaattt ttacaattga agtcaacggg gaaaaagtta
    20281 aaacagtaca agatatcaag gatcacgttg aggcagttga tatcatgttg gatgctttta
    20341 aagcgcacaa tattattaat gatattaacg atatcgatgg aacaggtcac cgtgttgtac
    20401 atggtggtga aaaattccct gaatcagtag ctattactga tgaggttgaa aaggaaattg
    20461 aagaattaag cgaattagca ccgcttcaca acccagctaa cttgatgggt attcgtgctt
    20521 tccgtaaatt attaccaaat atccctcatg tggcaatttt tgatacagca ttccatcaaa
    20581 caatgcctga aaaagcatat ctatatagct tgccatatca ttattataaa gattatggca
    20641 ttcgtaagta tggtttccat ggtacaagcc ataaatttgt atcacaaaga gcggcagaaa
    20701 tgttagataa gccaattgaa gatttacgta ttatttcatg tcacattggt aatggagcat
    20761 caattgcagc tatcgatggc ggtaaatcta ttgatacatc aatgggcttc acaccacttg
    20821 ctggtgtaac aatgggtact cgttctggta atattgaccc tgctttaatt ccatttatta
    20881 tggaaaaaac aggtaaaact gctgaacaag tattggaaat cttaaacaaa gaatcaggtt
    20941 tattaggatt atcaggtaca tctagtgatt tacgtgactt atcagaagaa gctgaatcag
    21001 gtaaagcacg ttcacaaatg gctttagatg tatttgcttc taaaattcac aaatatattg
    21061 gatcatatgc tgcgagaatg catggtgttg atgttatcgt atttacagca ggtatcggtg
    21121 aaaactcagt agaaattcgt gcgaaagttc ttgaaggctt agaatttatg ggtgtttact
    21181 gggatcctaa gaaaaatgaa aacttactac gtggtaaaga aggatttatt aactatcctc
    21241 attcaccagt taaagttgtc gttattccaa cagatgaaga aagtatgatt gcccgtgatg
    21301 ttatgacatt cggtggttta aaataatttt taaataaata ttaagcgtct atctccgcta
    21361 caattatatg tagg
//
LOCUS       DAXWFK010000027        20648 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.027, whole genome shotgun sequence.
ACCESSION   DAXWFK010000027 DAXWFK010000000
VERSION     DAXWFK010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20648
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.027"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(34..108)
                     /locus_tag="ACGEXS_000805"
     tRNA            complement(34..108)
                     /locus_tag="ACGEXS_000805"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(73..75),aa:Glu,seq:ttc)
     gene            complement(538..1821)
                     /locus_tag="ACGEXS_000806"
     CDS             complement(538..1821)
                     /locus_tag="ACGEXS_000806"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFF8011840.1"
                     /translation="MSSIVVVGTQWGDEGKGKITDFLAEQSDVIARFSGGNNAGHTIQ
                     FGGETYKLHLVPSGIFYKDKLAVIGNGVVVDPVALLKELDGLNERGIPTSNLRISNRA
                     QVILPYHLAQDEYEERLRGDNKIGTTKKGIGPAYVDKVQRIGIRMADLLEKETFERLL
                     KSNIEYKQAYFKGMFNETCPSFDDIFEEYYAAGQRLKEFVTDTSKILDDAFVADEKVL
                     FEGAQGVMLDIDHGTYPFVTSSNPIAGNVTVGTGVGPTFVSKVIGVCKAYTSRVGDGP
                     FPTELFDEDGHHIREVGREYGTTTGRPRRVGWFDSVVLRHSRRVSGITDLSINSIDVL
                     TGLDTVKICTAYELDGKEITEYPANLDQLKRCKPIFEELPGWTEDVTNVRTLEELPEN
                     ARKYLERISELCNVQISIFSVGPDREQTNLLKELW"
     gene            complement(2099..3499)
                     /gene="dnaB"
                     /locus_tag="ACGEXS_000807"
     CDS             complement(2099..3499)
                     /gene="dnaB"
                     /locus_tag="ACGEXS_000807"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFF8011841.1"
                     /translation="MDRMYEQNQMPHNNEAEQSVLGSIIIDPELINTTQEVLLPESFY
                     RGAHQHIFRAMMHLNEDNKEIDVVTLMDQLSTEGTLNEAGGPQYLAELSTNVPTTRNV
                     QYYTDIVSKHALKRRLIQTADSIANDGYNDELELDAILSDAERRILELSSSRESDGFK
                     DIRDVLGQVYETAEELDQNSGQTPGIPTGYRDLDQMTAGFNRNDLIILAARPSVGKTA
                     FALNIAQKVATHEDMYTVGIFSLEMGADQLATRMICSSGNVDSNRLRTGTMTEEDWSR
                     FTIAVGKLSRTKIFIDDTPGIRINDLRSKCRRLKQEHGLDMIVIDYLQLIQGSGSRAS
                     DNRQQEVSEISRTLKALARELECPVIALSQLSRGVEQRQDKRPMMSDIRESGSIEQDA
                     DIVAFLYRDDYYNRGGDEDDDDDGGFEPQTNDENGEIEIIIAKQRNGPTGTVKLHFMK
                     QYNKFTDIDYAHADMM"
     gene            complement(3531..3977)
                     /gene="rplI"
                     /locus_tag="ACGEXS_000808"
     CDS             complement(3531..3977)
                     /gene="rplI"
                     /locus_tag="ACGEXS_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFF8011842.1"
                     /translation="MKVIFTQDVKGKGKKGEVKEVPVGYANNFLLKKNYAVEATPGNL
                     KQLELQKKRAKQERQQEIEDAKALKETLSNIEVEVSAKTGEGGKLFGSVSTKQIAEAL
                     KAQHDIKIDKRKMDLPNGIHSLGYTNVPVKLDKEVEGTIRVHTVEQ"
     gene            complement(3974..5941)
                     /gene="gdpP"
                     /locus_tag="ACGEXS_000809"
     CDS             complement(3974..5941)
                     /gene="gdpP"
                     /locus_tag="ACGEXS_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP phosphodiesterase GdpP"
                     /protein_id="HFF8011843.1"
                     /translation="MNRQSTKKALLIPFVIMIITAIVLMGVWFIFNSLIALIASIVLV
                     VMIIVSIILFRQALMKMDSYVDGLSAQISTTNNKAIKHLPIGIIVLDENDHIEWVNQF
                     MTDHMEANVISESVNEVFPNILKQLDRVKSVEIEYNQYHFQVRYSENDHCLYFFDITE
                     QVQTNELYENSKPIIATLFLDNYDEITQNMNDTQRSEINSMVTRVISRWATEYNIFFK
                     RYSSDQFVAYLNQKILADLEESKFDILSQLREKSVGYRAQLTLSIGVGEGTENLIDLG
                     ELSQSGLDLALGRGGDQVAIKSINGNVRFYGGKTDPMEKRTRVRARVISHALKDILAE
                     GDKVIIMGHKRPDLDAIGAAIGVSRFAMMNNLEAYIVLNETDIDPTLRRVMNEIDKKP
                     ELRERFITSDDAWDMMTSKTTVVIVDTHKPELVLDENVLNKANRKVVIDHHRRGESFI
                     SNPLLIYMEPYASSTAELVTELLEYQPTEQRLTRLESTVMYAGIIVDTRNFTLRTGSR
                     TFDAASYLRAHGADTILTQHFLKDDVDTYINRSELIRTVKVEDNGIAIAHGSDDKIYH
                     PVTVAQAADELLSLEGIEASYVVARREDNLIGISARSLGSVNVQLTMEALGGGGHLTN
                     AATQLKGVTVEEAIAQLQQAITEQLSRSEDA"
     gene            complement(5956..6894)
                     /locus_tag="ACGEXS_000810"
     CDS             complement(5956..6894)
                     /locus_tag="ACGEXS_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HFF8011844.1"
                     /translation="MGVAYVFSKIQPKATIIATIALVFVALALYLVPGLGLIFALFAT
                     IPGIVLWNKSIQSFGISALITVIITTVLGNTFVLSAIILVLIASLIIGQLLKERTSKE
                     RILYVTTVAMSLISLIAFMLLQTFGRIPPSASIVKPFKQTLHEAITMSGADANMTQIL
                     EEGFRQATVQLPGFIIIITFLIVLINLIVTFPILRKFKIATPVFKPLFAWQMSGILLW
                     IYIIVIICLLFTGQPSVFQSILLNFQLVLSLVMYIQGLSVIHFFGKAKGLPNAVTILL
                     LVIGTILTPTTHIVGLLGVIDLSLNLKRIMKNNSKK"
     gene            complement(7185..8153)
                     /locus_tag="ACGEXS_000811"
     CDS             complement(7185..8153)
                     /locus_tag="ACGEXS_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFF8011845.1"
                     /translation="MTNYTVDTLNLGEFITESGEVIDNLRLRYEHVGYHGQPLVVVCH
                     ALTGNHLTYGTDDYPGWWREIIDGGYIPIHDYQFLTFDVIGSPFGSSSPLNDPHFPKK
                     LTLRDIVRANERGIQALGYDKINILIGGSLGGMQAMELLYNQQFEVDKAIILAATSRT
                     SSYSRAFNEIARQAIHLGGKEGLSIARQLGFLTYRSSKSYDERFTPDEVVAYQQHQGN
                     KFKEHFDLNCYLTLLDVLDSHNIDRGRTDVTHVFKNLETKVLTMGFIDDLLYPDDQVR
                     ALGERFKYHRHFFVPDNVGHDGFLLNFSTWAPNLYHFLNLKHFKRK"
     regulatory      complement(8213..8311)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(8516..8845)
                     /locus_tag="ACGEXS_000812"
     CDS             complement(8516..8845)
                     /locus_tag="ACGEXS_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFF8011846.1"
                     /translation="MITHMNMLILILLCGIVTLLIRIIPFIMISKVQLPDVVVRWLSF
                     IPITLFTALVIDSIIQQTPHGEGYTLNIPYIIALIPTVILSIITRSLTITIISGIVIM
                     AALRFFF"
     gene            complement(8842..9537)
                     /locus_tag="ACGEXS_000813"
     CDS             complement(8842..9537)
                     /locus_tag="ACGEXS_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFF8011847.1"
                     /translation="MTTHLSFRQGVQECIPTLLGYAGVGISFGIVASSQNFSILEIVL
                     LCLVIYAGAAQFIMCALFIAGTPISAIVLTVFIVNSRMFLLSMSLAPNFKTYGFWNRV
                     GLGSLVTDETFGVAITPYLKGEAINDRWMHGLNITAYLFWAISCVAGALFGEYISNPQ
                     TLGLDFAITAMFIFLAIAQFESITKSRLRIYIVLIIAVIVMMLSLSMFMPSYLAILIA
                     ATISAALGVMMEQ"
     gene            complement(10187..11473)
                     /gene="serS"
                     /locus_tag="ACGEXS_000814"
     CDS             complement(10187..11473)
                     /gene="serS"
                     /locus_tag="ACGEXS_000814"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFF8011848.1"
                     /translation="MLDIRLFRNEPDTVKSKIELRGDDPKVVDEILELDEQRRKLISA
                     TEEMKARRNKVSEEIALKKRNKENADDVIAEMRTLGDDIKEKDSQLNEIDNKMTGILC
                     RIPNLISDDVPQGESDEDNVEVKKWGTPREFSFEPKAHWDIVEELKMADFDRAAKVSG
                     ARFVYLTNEGAQLERALMNYMITKHTTQHGYTEMMVPQLVNADTMYGTGQLPKFEEDL
                     FKVEKEGLYTIPTAEVPLTNFYRNEIIQPGVLPEKFTGQSACFRSEAGSAGRDTRGLI
                     RLHQFDKVEMVRFEQPEDSWNALEEMTTNAEAILEELGLPYRRVILCTGDIGFSASKT
                     YDLEVWLPSYNDYKEISSCSNCTDFQARRANIRFKRDKAAKPELAHTLNGSGLAVGRT
                     FAAIVENYQNEDGTVTIPEALVPFMGGKTQISKPVK"
     misc_binding    complement(11563..11779)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(11852..13366)
                     /gene="hutH"
                     /locus_tag="ACGEXS_000815"
     CDS             complement(11852..13366)
                     /gene="hutH"
                     /locus_tag="ACGEXS_000815"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HFF8011849.1"
                     /translation="MTLYLDGETLTIEDIKSFLQQQSKIEIIDDALERVKKSRAVVER
                     IIENEETVYGITTGFGLFSDVRIDPTQYNELQVNLIRSHACGLGEPFSKEVALVMMIL
                     RLNTLLKGHSGATLELVRQLQFFINERIIPIIPQQGSLGASGDLAPLSHLALALIGEG
                     KVLYRGEEKDSDDVLRELNRQPLNLQAKEGLALINGTQAMTAQGVISYIEAEDLGYQS
                     EWIAALTHQSLNGIIDAYRHDVHAVRNFQEQINVAARMRDWLEGSTLTTRQSEIRVQD
                     AYTLRCIPQIHGASFQVFNYVKQQLEFEMNAANDNPLIFEEANETFVISGGNFHGQPI
                     AFALDHLKLGVSELANVSERRLERLVNPQLNGDLPAFLSPEPGLQSGAMIMQYAAASL
                     VSENKTLAHPASVDSITSSANQEDHVSMGTTAARHGYQIIENARRVLAIECVIALQAA
                     ELKGVEGLSPKTRRKYDEFRSIVPSITHDRQFHKDIEAVAQYLKQSIYQTTACH"
     gene            13692..14504
                     /locus_tag="ACGEXS_000816"
     CDS             13692..14504
                     /locus_tag="ACGEXS_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFF8011850.1"
                     /translation="METLNSINIPKRKEDSHKGDYGKILLIGGSANLGGAIMLAARAC
                     VFSGSGLITVATHPTNHSALHSRCPEAMVIDINDTKMLTKMIEMTDSILIGPGLGVDF
                     KGNNAITFLLQNIQPHQNLIVDGDAITIFSKLKPQLPTCRVIFTPHLKEWERLSGIPI
                     EEQTYERNREAVDRLGATVVLKKHGTEIFFKDEDFKLTIGSPAMATGGMGDTLAGMIT
                     SFVGQFDNLKEAVMSATYTHSFIGENLAKDMYVVPPSRLINEIPYAMKQLES"
     gene            complement(14591..17254)
                     /gene="gyrA"
                     /locus_tag="ACGEXS_000817"
     CDS             complement(14591..17254)
                     /gene="gyrA"
                     /locus_tag="ACGEXS_000817"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFF8011851.1"
                     /translation="MAELPQSRINERNITSEMRESFLDYAMSVIVARALPDVRDGLKP
                     VHRRILYGLNEQGMTPDKSYKKSARIVGDVMGKYHPHGDLSIYEAMVRMAQDFSYRYP
                     LVDGQGNFGSMDGDGAAAMRYTEARMTKITLELLRDINKDTIDFIDNYDGNEREPSVL
                     PARFPNLLANGASGIAVGMATNIPPHNLTELINGVLSLSKNPDISIAELMEDIEGPDF
                     PTAGLILGKSGIRRAYETGRGSIQMRSRAVIEERGGGRQRIVVTEIPFQVNKARMIEK
                     IAELVRDKKIDGITDLRDETSLRTGVRVVIDVRKDANASVILNNLYKQTPLQTSFGVN
                     MIALVNGRPKLINLKEALVHYLEHQKTVVRRRTQYNLRKAKDRAHILEGLRIALDHID
                     EIISTIRESDTDKVAMESLQQRFKLSEKQAQAILDMRLRRLTGLERDKIEAEYNELLN
                     YISELEAILADEEVLLQLVRDELTEIRDRFGDDRRTEIQLGGFEDLEDEDLIPEEQIV
                     ITLSHNNYIKRLPVSTYRAQNRGGRGVQGMNTLEEDFVSQLVTLSTHDHVLFFTNKGR
                     VYKLKGYEVPELSRQSKGIPVVNAIELENDEVISTMIAVKDLESEDNFLVFATKRGVV
                     KRSALSNFSRINRNGKIAISFREDDELIAVRLTSGQEDILIGTSHASLIRFPESTLRP
                     LGRTATGVKGITLREGDEVVGLDVAHANSVDEVLVVTENGYGKRTPVNDYRLSNRGGK
                     GIKTATITERNGNVVCITTVTGEEDLMIVTNAGVIIRLDVADISQNGRAAQGVRLIRL
                     GDDQFVSTVAKVKEDAEDETNEDEQSTSTVSEDGTEQQREAVVNDETPGNAIHTEVID
                     SEENDEDGRIEVRQDFMDRVEEDIQQSSDEE"
     gene            complement(17291..19225)
                     /gene="gyrB"
                     /locus_tag="ACGEXS_000818"
     CDS             complement(17291..19225)
                     /gene="gyrB"
                     /locus_tag="ACGEXS_000818"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFF8011852.1"
                     /translation="MVTALSDVNNTDNYGAGQIQVLEGLEAVRKRPGMYIGSTSERGL
                     HHLVWEIVDNSIDEALAGYANQIEVVIEKDNWIKVTDNGRGIPVDIQEKMGRPAVEVI
                     LTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSQDLEVYVHRNETIYHQAYKKGVPQF
                     DLKEVGTTDKTGTVIRFKADGEIFTETTVYNYETLQQRIRELAFLNKGIQITLRDERD
                     EENVREDSYHYEGGIKSYVELLNENKEPIHDEPIYIHQSKDDIEVEIAIQYNSGYATN
                     LLTYANNIHTYEGGTHEDGFKRALTRVLNSYGLSSKIMKEEKDRLSGEDTREGMTAII
                     SIKHDDPQFEGQTKTKLGNSEVRQVVDKLFSEHFERFLYENPQVARTVVEKGIMAARA
                     RVAAKKAREVTRRKSALDVASLPGKLADCSSKSPEECEIFLVEGDSAGGSTKSGRDSR
                     TQAILPLRGKILNVEKARLDRILNNNEIRQMITAFGTGIGGDFDLAKARYHKIVIMTD
                     ADVDGAHIRTLLLTFFYRFMRPLIEAGYVYIAQPPLYKLTQGKQKYYVYNDRELDKLK
                     SELNPTPKWSIARYKGLGEMNADQLWETTMNPEHRALLQVKLEDAIEADQTFEMLMGD
                     VVENRRQFIEDNAVYANLDF"
     gene            complement(19235..20347)
                     /gene="recF"
                     /locus_tag="ACGEXS_000819"
     CDS             complement(19235..20347)
                     /gene="recF"
                     /locus_tag="ACGEXS_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFF8011853.1"
                     /translation="MKLNTLQLENYRNYDEVTLKCHPDVNILIGENAQGKTNLLESIY
                     TLALAKSHRTSNDKELIRFNADYAKIEGELSYRHGTMPLTMFITKKGKQVKVNHLEQS
                     RLTQYIGHLNVVLFAPEDLNIVKGSPQIRRRFIDMELGQISAVYLNDLAQYQRILKQK
                     NNYLKQLQLGQKKDLTMLEVLNQQFAEYAMKVTDKRAHFIQELESLAKPIHAGITNDK
                     EALSLNYLPSLKFDYAQNEAARLEEIMSILSDNMQREKERGISLFGPHRDDISFDVNG
                     MDAQTYGSQGQQRTTALSIKLAEIELMNIEVGEYPILLLDDVLSELDDSRQTHLLSTI
                     QHKVQTFVTTTSVDGIDHEIMNNAKLYRINQGEIIK"
     gene            complement(20344..20589)
                     /gene="yaaA"
                     /locus_tag="ACGEXS_000820"
     CDS             complement(20344..20589)
                     /gene="yaaA"
                     /locus_tag="ACGEXS_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFF8011854.1"
                     /translation="MIILVQEVVVEGDINLGQFLKTEGIIESGGQAKWFLQDVEVLIN
                     GVRETRRGKKLEHQDRIDIPELPEDAGSFLIIHQGEQ"
BASE COUNT         6248 a         4135 c         3124 g         7141 t
ORIGIN      
        1 cgtgttaacc gctacactac gagacctaaa aaatggtgac tcctacggga ctcgaacccg
       61 tgttaccgcc gtgaaagggc ggtgtcttaa ccgcttgacc aaggagccgt tcttaagcac
      121 aagatagatt atagcacagc caatcttgcc atgacaagat attttttaca taattttgct
      181 attatttttt gaagaatgtt ataagtgaaa aattgcattt tattggcagt ctatttatta
      241 taataattac gctatttttg aaatttttct attttaggtg ctttcattta cacttacgtc
      301 tactctattt taatagctat tttcgacatc agacttctta cattcaagta tttcatctta
      361 attttcgtta aatatcatct aatcttctaa attatgacta gcaaatatcg cctttacgtt
      421 agtatttttt atctaaataa tcgaatcgat acagaagcaa gcataacaaa agcctcctac
      481 caataaagat agaaggctca tgtatttata atcattgtgt atgacttata taaagttcta
      541 ccacaattct tttaataggt ttgtttgttc tctatctgga ccaactgaga agatagaaat
      601 ttgtacatta cataattctg aaatacgctc taaatattta cgtgcatttt caggtaattc
      661 ttctaaagta cgcacatttg ttacgtcttc tgtccaacct ggtaactctt caaagattgg
      721 tttacaacgt tttaattgat ctaagtttgc tgggtactca gtaatttctt taccgtctaa
      781 ttcataagct gtacagattt tcactgtgtc taggcctgtt aaaacatcga ttgagttaat
      841 agataaatct gtaataccac ttacacgacg agagtgacgt aatacaactg aatcaaacca
      901 acctacacga cgtggacgtc ctgttgttgt accgtattca cgaccaacct ctctaatatg
      961 atgtccatct tcatcgaata attcagtagg gaatggacca tcaccaacac gtgatgtata
     1021 agctttacat acaccaatta cctttgaaac gaatgtagga cctacacctg taccaacagt
     1081 aacgttacct gcaattggat tacttgatgt aacgaatgga tatgtaccat ggtcgatatc
     1141 taacattaca ccttgcgcac cttcgaaaag taccttttca tctgctacaa atgcatcgtc
     1201 taagattttt gatgtgtctg ttacaaattc ttttagacgt tgacctgctg cataatattc
     1261 ttcaaagata tcatcaaatg atggacatgt ttcgttaaac atacctttga aatatgcttg
     1321 tttatattca atgtttgatt ttaataatct ttcgaatgtt tctttttcaa gtaaatctgc
     1381 catacgaata ccgatacgtt gaactttgtc tacatatgct ggaccgatac cttttttagt
     1441 tgtaccaatc ttattgtcac cacgtaaacg ttcttcatat tcatcttgtg ctaagtgata
     1501 tggtaaaatc acttgcgcac gattagatat acgtaaatta cttgtaggaa tgccacgttc
     1561 atttaatccg tctaattctt tcaatagtgc aactggatca acaacgactc cgttaccgat
     1621 taccgctaat ttgtctttgt aaaagatacc agatggtact aaatgtaatt tatatgtttc
     1681 tccgccaaat tgaatggtat ggcctgcatt attaccacct gaaaaacgcg cgataacatc
     1741 tgactgttct gccaagaaat ccgttatttt tccttttcct tcgtctcccc attgtgtccc
     1801 aactactacg attgatgaca tgtgagcacc tccaagtttt tctcatttaa ccatttgata
     1861 ttctaccaat atcgagacca taatgcaaat aaaaatcgaa cattgatttt cggccttttt
     1921 aaaagaaaaa tcattttaac aaatcgtttt caacgtccta aaaacccttt aacagaggcg
     1981 ttgacagtac taatttatca ctttcaatac acaaacttaa catatatcaa cccgttcaga
     2041 acaaaataaa aaccgtacaa ttttatcatc ttaatgatta ttgtacggaa aaactttttt
     2101 acatcatatc tgcatgtgca taatcgatat cggtaaattt attatattgt ttcataaaat
     2161 gtaacttaac tgtgcctgtt ggaccgttac gttgcttagc aatgataatt tcaatttcac
     2221 cgttttcatc attcgtttgt ggctcgaaac caccatcatc gtcatcatct tcatcgccgc
     2281 cacggttata gtaatcatca cggtataaga atgcaacgat atcggcatct tgctcaatcg
     2341 aaccagattc acgaatatca ctcatcattg gacgtttatc ttgtcgttgt tcaacaccac
     2401 gagataactg acttaatgcg ataactggac attctaattc acgggctaat gcttttaatg
     2461 tacgagagat ttcagaaact tcctgttgtc tgttatcgga cgcacgtgaa ccactacctt
     2521 gaatcaactg taagtagtca atcacaatca tgtctaagcc atgttcttgc tttaatcgac
     2581 gacatttaga acgtaaatca ttaattcgaa tacccggtgt atcatcaata aaaatcttcg
     2641 tacgtgataa tttacctacc gctatagtaa aacgactcca atcttcctca gtcatagtac
     2701 ccgttcttaa gcggtttgag tcaacatttc cagaactaca aatcatacgt gtggctaact
     2761 gatcagcacc catctctagc gagaaaatac caactgtata catatcttca tgcgttgcaa
     2821 ctttttgtgc aatattaagt gcgaacgcag tcttacctac agatggacgc gctgcaagga
     2881 taattaaatc atttcggttg aaccctgctg tcatttggtc taaatctcga tatcctgtag
     2941 gtatacctgg tgtttgacca ctattttgat caagctcttc agctgtttca tacacttgtc
     3001 ctaagacgtc tcgaatgtct ttaaagccat cgctttcacg agaagatgat agctctaaaa
     3061 ttcgacgttc tgcatcactt aaaatcgcat ctagttcaag ttcatcatta tatccatcat
     3121 tggcaatact atctgcagtt tgaatcaatc tacgttttaa tgcatgctta gaaacgatat
     3181 cagtataata ctgaacattt cgcgtcgttg gtacatttgt agataactct gcaagatatt
     3241 gcgggccacc cgcttcattc aacgtacctt ccgtcgataa ttgatccatc aatgttacaa
     3301 catcaatttc tttattatct tcatttaagt gcatcattgc acggaaaata tgttgatggg
     3361 cacccctata aaacgactca ggaagcaaaa cttcctgagt agtattaatc aattctggat
     3421 ctataataat tgaacctaag acagactgtt cagcttcatt gttatgcggc atttgatttt
     3481 gctcatacat tctatccatg aatggttaca cctcttattt caatccaact ttattgttca
     3541 actgtgtgta cgcgaattgt accttcaact tctttatcta atttaacagg tacattcgta
     3601 tatcctaggg aatgaattcc atttggtaaa tccattttac gtttatcaat tttaatatca
     3661 tgttgtgctt ttagtgcttc ggcaatttgt tttgtactta ctgacccaaa caatttacca
     3721 ccttcaccag tttttgctga tacttcaact tcaatgtttg ataacgtttc ttttaatgct
     3781 ttagcatctt caatttcttg ttggcgttct tgttttgcac gttttttctg taactctaat
     3841 tgtttaaggt tacctggtgt tgcttctaca gcataattct ttttcaataa gaagttattt
     3901 gcataaccta ctggtacttc tttaacttca ccttttttac ctttaccttt aacatcttgt
     3961 gtaaaaatta ctttcatgca tcttcactcc tacttaattg ttctgtaatt gcttgttgta
     4021 attgtgctat cgcctcttcg actgtcacac ctttaagttg tgttgccgca ttggttaaat
     4081 gtccaccgcc accaagtgct tccattgtta actggacatt tactgaaccg agtgaacgcg
     4141 cagatatacc aatcagatta tcttcacgtc tcgcaacaac atatgatgct tcaatacctt
     4201 ctaaacttaa cagttcatct gctgcttgtg caactgttac tggatgataa attttatcgt
     4261 ctgaaccatg cgcaatggct atgccattat cttcaacttt tacagttcga attaattcag
     4321 atcgattaat gtaagtatcc acatcatctt ttaagaaatg ttgcgttaaa atcgtatctg
     4381 caccatgtgc acgtaaataa ctcgctgcat cgaatgttct tgatcctgtt cgtaatgtaa
     4441 agtttcttgt atctacaata atacctgcat acatcactgt tgattcaaga cgtgttaaac
     4501 gttgttctgt tggttgatat tccagtaact ctgttaccaa ttcagctgtc gaacttgcgt
     4561 atggttccat atatatcaac aatggattag agatgaagct ttcaccacgt ctatgatgat
     4621 cgataacaac tttacggttt gctttattta agacattttc atctaaaacc agttccggtt
     4681 tatgcgtatc aacaatcact acggttgtct tagatgtcat catatcccaa gcatcatctg
     4741 atgtaataaa tcgctctctt aactctggct ttttatctat ttcgttcatc acgcgtcgta
     4801 atgttggatc aatgtcagtc tcatttaata cgatgtatgc ttctaaatta ttcatcattg
     4861 caaatctaga cacaccgatt gctgcaccaa ttgcatctaa gtcaggacgt ttatgtccca
     4921 tgataatgac tttgtcaccc tctgcaagga tatcttttaa cgcatgtgag atcacacgtg
     4981 ctcttacacg agtacgtttc tccatcgggt cagtcttacc gccatagaaa cgcacattac
     5041 cattaatact tttaattgca acttggtcgc caccgcgtcc taatgctaag tctaggcctg
     5101 attgtgataa ttcacctaag tcgattaaat tttcagtacc ttcaccaaca ccgatactta
     5161 atgttaattg ggcacgataa ccaacacttt tttcacgtaa ttgactcaag atatcaaatt
     5221 tagattcttc taagtcagct aatatttttt gatttaaata ggctacgaat tgatcggaac
     5281 tgtatctttt gaaaaatata ttatactcag ttgcccatcg actaatgaca cgcgttacca
     5341 ttgagttgat ttccgaacgc tgcgtatcat tcatattttg cgtaatctca tcgtagttat
     5401 ctaaaaataa tgtcgcaatg attggtttag aattttcata tagttcattt gtttgtactt
     5461 gttcagttat atcaaagaaa tagaggcagt gatcattctc agaataacgt acttggaaat
     5521 gatactgatt atattctatt tcaacggatt tcactctatc taattgcttt aaaatgtttg
     5581 gaaatacttc atttacagat tcagaaatga cattcgcttc catatgatct gtcataaatt
     5641 ggttaaccca ttcgatgtga tcattttcat ctaaaacaat gataccaatt ggtaaatgtt
     5701 tgattgcttt attatttgtt gttgaaattt gagcactcaa accatctaca taactatcca
     5761 ttttcattaa agcttgtctg aataaaatga tgctaacaat aatcatcacg acaagaacga
     5821 tagatgcaat tagtgctata agactattaa agataaacca tacacccatt aaaacaattg
     5881 ctgtgatgat catgatgaca aatggtatta gtaaagcttt cttagtggac tgccgattca
     5941 ttattccacc tctattcact ttttagaatt atttttcatg attcgcttca aattcaaact
     6001 taaatcgata acaccaagta gtcctacaat atgtgtcgta ggtgtcagta ttgtaccgat
     6061 aaccaatagt aaaatcgtta ctgcattcgg caaacctttc gctttaccaa agaaatgaat
     6121 aacacttaaa ccttgaatat acattactaa tgataacaca agttggaagt ttaaaagaat
     6181 gctctggaac acactcggtt gacctgtaaa taataaacat atgataacaa taatgtatat
     6241 ccataataaa ataccgctca tttgccacgc gaaaagtggc ttaaatacag gtgtagcgat
     6301 tttaaatttt cgtaaaatcg gaaatgtaac gattaagtta attaagacga ttaaaaatgt
     6361 aatgataatg atgaaacctg gtaattgaac ggtcgcttgt ctaaaccctt cttctaatat
     6421 ttgggtcata ttcgcatcgg caccgctcat cgtaatcgct tcatgtaatg tttgcttgaa
     6481 aggttttact atgctcgctg atggtggaat ccttccgaat gtttgtagta acataaaagc
     6541 gattaatgaa attaagctca tcgctactgt tgttacgtat aatattcttt ctttagacgt
     6601 tctttctttg agcaattgac caataattaa acttgcaatt aagactaata tgatggcact
     6661 taaaacgaaa gtattaccta aaacagttgt tataattact gtaataagtg cactaatccc
     6721 gaaagattgt attgatttat tccataaaac gatacctggt atggttgcaa ataatgcaaa
     6781 tattagtcct aaaccaggca ctagatataa agctaaagcg acaaatacca acgcaatcgt
     6841 tgcaattatt gttgctttag gttgtatttt tgaaaacaca taagccactc ccatattttt
     6901 aactatagct attattttaa cctctttaat gaaaattaac aatttataga ttgtatgctt
     6961 ctatttcatt taattgaata ataactttca tgttttataa gtaattaaca tactcatttg
     7021 aatcgctttt gtgtgctttc attttcaaca tgattattta atcccactac atagcaatca
     7081 agcttgattt agatttacaa tacatttcca ctctcatgta ctctagatgt ttttgaatat
     7141 gataactgtg atttagtggc ttcattcttt gaaaatatat attattactt acgcttaaaa
     7201 tgctttaaat ttaagaaatg atataagtta ggtgcccagg tactaaagtt tagtaggaat
     7261 ccatcatgcc caacattatc aggcacgaag aaatgacgat gatatttaaa acgttcacct
     7321 aatgcacgaa cttgatcatc cggatatagc aaatcatcta tgaaccccat cgttaacact
     7381 tttgtttcta aatttttaaa aacatgcgtt acgtctgtgc gacctcggtc aatgttgtga
     7441 ctatccaata catctagcag tgtcagataa caattcaaat caaaatgttc tttaaattta
     7501 ttaccttgat gttgttggta tgcgactact tcatccggcg tgaaacgttc atcataactt
     7561 tttgatgatc gatatgtcaa aaaacctaat tggcgtgcaa tacttagacc ttccttacca
     7621 ccaagatgaa tggcttgcct tgcaatttca ttgaaagctc tactataaga tgatgttcga
     7681 cttgttgcag caaggataat ggctttatct acttcaaact gttgattgta gagtagttcc
     7741 attgcttgca tacctccaag acttccccct attaaaatat taatcttatc ataaccaagg
     7801 gcttgtatac ctcgttcatt cgctctgact atatctctta atgttaattt tttaggaaaa
     7861 tgagggtcgt ttaaaggtga acttgaaccg aaaggactac caataacatc aaatgttaaa
     7921 aattgataat cgtgaatggg tatatatccc ccatcaataa tttctcgcca ccaacccgga
     7981 taatcatctg ttccatatgt taaatgattg ccagttaatg catgacaaac tacaactaat
     8041 ggttgtccat gataaccgac atgctcatat ctcaaacgca agttatctat gacttcccca
     8101 gattctgtaa taaattcccc taaatttaaa gtatctactg tgtaatttgt cattgttctt
     8161 tcctccttaa acaaaaaaac ttctcaccct attgaaaagt aagaagtctt tatacttatc
     8221 attcgagtaa ctcgttggtt ttagcaccgt gctataaagt cggttgctga agtatcacag
     8281 ggccaagtcc ctcaacttct cacaataaga aatatgaaat tgttatgtgt tagttgagat
     8341 tcagtgatga attactttta tcatttaaaa tgttgttatc attgtcatgc gttagccaaa
     8401 tcgcttacgt atacacgatt cccaatctta acatagacga tttgtatatc agaattttct
     8461 gattactaac agtttaccta agtttaaata tctgttcaat gattttcagt tatttttaaa
     8521 agaaaaatcg taatgctgcc atgataacaa tcccactaat aattgtaata gttaaactac
     8581 gcgtgattat agataaaata accgtcggaa tgagcgcgat aatgtaaggg atgtttaatg
     8641 tatacccctc accatgaggc gtctgttgaa taatgctgtc aatgacaagt gccgtaaata
     8701 gtgtgattgg gataaatgat agccatcgaa ccacgacatc aggcaattgc acttttgaaa
     8761 tcatgataaa aggtataatt cgaattaata gcgttacgat accacacaat aaaataagta
     8821 ttaacatgtt catatgagtt atcattgttc catcatcact cctaacgctg ctgaaattgt
     8881 ggctgcaatt aatattgcta gatatgaagg cataaacata cttagcgata acatcattac
     8941 tatgacggca ataatgagta ctatgtaaat tcttaatcgc gatttagtaa ttgattcaaa
     9001 ttgcgcaatg gccaaaaaga taaacatagc cgtgatagca aaatctaacc ctagcgtttg
     9061 cggatttgag atatattcgc caaataaagc cccagctaca catgaaattg cccaaaataa
     9121 atatgctgtg atgttaagac catgcatcca acgatcattg atagcttctc cttttaaata
     9181 aggtgtaatg gcgacgccaa acgtttcgtc agttactaat gaacctaatc caacacggtt
     9241 ccaaaaccca tatgtcttga agtttggtgc aagcgacata cttaaaagga acattcttga
     9301 atttacgata aatacagtta gtacaatcgc tgatataggt gtacctgcta taaacaacgc
     9361 gcacataata aattgcgcag caccggcata tataacaaga cataacaaga caatttctaa
     9421 aatactaaag ttttgagacg aagccacaat accaaatgaa ataccaacac cggcataacc
     9481 caataatgtt gggatacact cttgcacgcc ttgtctaaaa cttaaatgtg ttgtcatctc
     9541 aattacctcc tttgccactt tatatttata taattacgaa aaaataatat acctagtcta
     9601 atacagttct ataccctaag caataacatt agacatcagt ttgtctgagg ttagacatac
     9661 cggagtcttt agtcagcttc atattaactt tttatttttg agaattttca attttttatt
     9721 taagactacc tccatatttt ctatggattt gtagttgttt ttaagtatca attttataaa
     9781 tttttatatc tgatgatgag tctgggatat tgattcatgt accactccct tataatcatc
     9841 ccctccccct accctactcc atcgatataa ctcatactac atatcaacga aatcagtatt
     9901 ttatcgcttc ctttcctata ttagtgatgc tcaaacttgt tacgttttag attgttttag
     9961 ttcatcataa ttatcccgta ttgttgctat aatgaaatgc gttcacccca ttaaaccaca
    10021 aacttaattt attgttgtta tgtgcattgg ctcactatta tacttttaca gcacaaaaaa
    10081 agtggcgaca gcttcgtcac cactttttaa aatattattt aaagtatctt gcccttgctt
    10141 taagtatacg tagatatata ctttttaaag cttgtagcta aagcctttat ttaactggtt
    10201 ttgaaatttg tgttttacca cccataaatg gtactaatgc ttctggaatt gttactgttc
    10261 catcttcatt ttggtaattt tcaacaatag cagcaaatgt acgtccaact gctaaaccac
    10321 taccatttaa tgtatgtgct aattctggtt tagctgcttt gtcacgcttg aagcggatgt
    10381 tagcacgacg cgcttggaaa tccgtacagt ttgagcatga actaatttct ttataatcat
    10441 tgtagcttgg taaccaaact tctaaatcat atgttttgct tgcactaaat ccaatatcac
    10501 ctgtacataa aataacacga cggtatggta aacctaactc ttctagaatt gcttctgcgt
    10561 ttgttgtcat ttcttctaaa gcattccatg aatcttcagg ttgttcaaaa cgtaccattt
    10621 ccactttatc gaattgatgt aaacgaatta atcctcttgt atctctacct gctgatcctg
    10681 cttcactacg gaaacatgca gattgaccag tgaatttttc aggaagtaca cctggttgaa
    10741 taatttcatt acggtagaaa ttcgttaatg gtacttcagc agttggaatt gtatataatc
    10801 cttctttttc tactttaaat aaatcttctt caaatttagg taattgacct gtaccataca
    10861 ttgtatctgc gttcacaagc tgtggtacca tcatttctgt ataaccatgt tgtgttgtat
    10921 gttttgtaat catatagttc attaaagcac gctctaattg cgcaccttca tttgttaaat
    10981 atacaaaacg cgcacctgaa acttttgctg cacgatcaaa atcagccatt ttcaattctt
    11041 ctacaatatc ccaatgtgct ttgggttcaa atgaaaactc acgtggtgta ccccactttt
    11101 taacttcaac gttatcttca tcagattcac cttgaggtac atcatcactt attaaatttg
    11161 gaatacgaca aaggatacct gtcattttat tatcaatttc atttaattga ctatcttttt
    11221 ctttaatatc gtcacctaat gtgcgcattt cagcaatcac atcatcagca ttttctttat
    11281 tacgtttttt taatgcgatt tcttcgctta ctttattacg acgtgctttc atttcttctg
    11341 ttgcactaat taatttacgt cgttgctcat ccaattctaa aatttcatct acaacttttg
    11401 gatcatctcc acgtaattca attttgctct taactgtgtc aggctcattt ctgaataatc
    11461 taatgtctaa cattaacctt catcctttcc caaataatta tcatttatta tggaatgacg
    11521 tacgtcttta ttttttagaa aataaaaaaa gaccacatcc ctacaaggga cgtggtctac
    11581 gcgttgccac cctatttaac aatttaagtt ataaagatac actaaaccta aattgcactt
    11641 cactaaaata acggttatca ccgattgttc ttttaaatta agtaggtaga ttcatatata
    11701 tgttgattct tgttcacact aaccacaagc tctctgatat cgaacactat atattacttg
    11761 tcctacgaac aatgtcttat taagttattt ttaatatagc aaactatatt tgctttttca
    11821 agtaacgatt tcaaacatca ctcatgtcga tttagtgaca tgcagtcgtt tgataaattg
    11881 attgctttaa atactgtgca accgcttcaa tatctttatg aaattgacga tcatgtgtaa
    11941 tggatggcac gatacttcga aactcatcat acttgcgacg tgtttttggt gataatcctt
    12001 caacaccttt taactctgct gcttgtaatg caataacaca ttcgattgcc agcacacgtc
    12061 ttgcattttc aataatttga taaccatgtc tagcagctgt agttcccata gatacgtgat
    12121 cttcttggtt cgcagatgaa gtgatagaat caacactcgc tggatgcgct aaagttttat
    12181 tttcagaaac gagacttgca gcagcatatt gcataatcat cgcgccactt tgcaatcctg
    12241 gctctggact aagaaatgct ggtaaatcac catttaattg aggatttact agtcgctcta
    12301 gacgacgttc cgatacgttt gctaattcac ttacacctaa tttaagatga tctaatgcaa
    12361 aagcaatagg ttgtccatgg aagttaccac ctgaaataac aaacgtttca tttgcttcct
    12421 caaatataag tggattatca ttagccgcat tcatttcaaa ttctaattgc tgtttaacat
    12481 aattgaatac ttgaaaactc gcgccatgga tttgtggtat acaacgcaac gtatatgcat
    12541 cttgtacacg tatttctgat tgtcgcgtcg ttaatgttga tccttctaac caatcacgca
    12601 tacgcgctgc cacattaatc tgttcttgaa aattacgaac tgcgtgcaca tcatgtcgat
    12661 atgcatctat aatgccatta agagactgat gcgttaatgc agcaatccat tcagattggt
    12721 aacctaaatc ttctgcttct atataactaa tgacaccttg agctgtcata gcttgcgtac
    12781 cattaatcaa tgctaaacct tctttagcct gaaggttcaa aggttgtcta tttaattctc
    12841 ttaatacatc gtcactatcc ttttcttccc ctctgtacaa tactttccct tcaccaatta
    12901 atgctaatgc taaatgtgat aatggcgcta aatctcctga tgcaccgaga gagccttgct
    12961 gtgggattat cggtataata cgttcattta taaaaaattg taattgtctc actaattcta
    13021 aagtggcacc tgaatgacct tttaataatg tattcaatcg taaaatcatc atgactaatg
    13081 ctacttcttt tgaaaatggc tcacctagtc cacaggcatg tgagcgtatc agattcactt
    13141 gtaattcatt atattgcgtc gggtctatac gtacatcact aaataaccca aaacctgtag
    13201 taataccgta aaccgtttcc tcattttcaa taatacgttc aactaccgct ctactttttt
    13261 tgacacgttc taacgcatca tcaataattt caatctttga ttgttgttgt aaaaatgatt
    13321 taatatcctc aattgttagt gtttcaccat ctaaatataa agtcatatat gttaccccct
    13381 tgtttatatt aagtaaccca tccttcttga agtatacgtt ttcattttta ttgaaacaat
    13441 ggttttacgt acatttataa cctattatca gagcactatt gtagtgcgtt aaaggatatt
    13501 aagattgttg taagcatatt taataattta tctattgacg aattgcatat acaggtatag
    13561 tattttctat tgtatttaac gacaaataat aatgaattca gaaatttata atacattttg
    13621 ttaaaagtta ctatatattt ttaaaattga ataaattcgg aaaaggcttt tacatgggag
    13681 gttatatcac tatggaaacg ttaaattcta ttaacattcc taagcgtaaa gaagattcac
    13741 ataaaggtga ttatggcaaa attttattaa ttggtggatc tgctaactta ggtggtgcca
    13801 ttatgttagc ggctcgtgca tgtgtattta gcggtagtgg tttaatcact gtagctacac
    13861 atccaacaaa tcattcagca ttacattctc gttgcccaga agcgatggtt attgatatta
    13921 atgatacgaa aatgttgacg aaaatgattg aaatgactga cagtatacta attggtccag
    13981 gtcttggcgt tgatttcaaa ggaaataatg ccattacatt cctactacaa aatatacaac
    14041 cgcatcaaaa tttaatcgta gacggcgatg cgattacaat ctttagtaaa ctgaaaccgc
    14101 aattacctac atgtcgtgtg atctttacac cacacctcaa agaatgggaa cgattaagtg
    14161 gtattcctat tgaggaacag acatatgagc gtaatcgtga agcagttgat cgtttaggtg
    14221 caactgttgt acttaaaaaa catggtactg aaattttctt taaagatgaa gactttaaat
    14281 tgacaatcgg tagcccagca atggcgactg gtggtatggg cgatacactt gctggtatga
    14341 ttacaagctt tgtcggtcaa tttgataact taaaagaagc ggttatgagt gccacatata
    14401 cacatagttt tattggcgaa aaccttgcaa aagatatgta tgtggtgcca ccatcaagac
    14461 ttatcaatga aataccttac gcaatgaaac aattagaaag ttagtcatta ctaatcattg
    14521 aatatagtaa agcattactt tctagcataa aaataagact cccctacata tagggaagtc
    14581 ttatttttta ttattcttca tctgatgatt gttgtatatc ttcttcaaca cgatccatga
    14641 aatcttgtct tacttcaata cgtccatctt catcattttc ttctgaatca atcacttcag
    14701 tatgaattgc atttcctggt gtttcatcat ttacaaccgc ttcacgttgt tgttcagtac
    14761 catcttcaga tacagttgaa gtagattgct catcttcatt cgtttcatct tctgcatctt
    14821 cttttacttt agcaaccgtt gaaacaaatt gatcatcacc taagcgaatt aagcgaacac
    14881 cttgtgctgc acgaccattt tgagaaatat ctgcaacatc tagtcgaata atgacacctg
    14941 cattagtaac aatcattaaa tcttcttcac cagttactgt agtgatacat acaacattac
    15001 cattacgctc agtaatcgta gctgttttaa tacctttacc accacgattt gataagcgat
    15061 agtcattaac tggcgtacgt ttaccataac cattttcagt aactactaat acttcatcaa
    15121 cactgtttgc atgagctaca tcaagcccta caacttcgtc accttcacga agtgtaatac
    15181 ctttcacacc cgttgctgta cggcctaaag gacgtaatgt tgattcaggg aatcgaatta
    15241 atgatgcatg tgatgtacca atcaagatat cttcttgacc acttgttaag cgaactgcaa
    15301 ttaactcatc atcttctctg aacgaaatcg caatcttacc atttctattt attcttgaga
    15361 agttacttaa tgctgaacgt ttaacgacac cacgtttagt tgcaaacact aagaagttgt
    15421 cttcactttc aaggtcttta acagcaatca ttgtactaat gacttcatca ttttcaagtt
    15481 caatagcatt cactacagga atacctttag actgtcttga taactcaggc acttcgtaac
    15541 ctttaagttt gtatacacga cctttgttag taaagaacaa tacatggtca tgtgtactta
    15601 aagttaccaa ttgactgaca aaatcttctt ccaatgtatt cataccttga acaccacgac
    15661 caccacggtt ttgagcacga tatgtagata ccggcaaacg tttaatgtag ttattatggc
    15721 ttagtgtaat tactatttgt tcttctggaa ttaagtcttc gtcctctaag tcttcaaatc
    15781 cacctaattg aatttctgta cgacgatcat caccgaaacg atctctaatt tcagtcaatt
    15841 catctctaac taactgtaat aacacttctt catcagctaa gattgcttct aattcactaa
    15901 tataatttaa taactcatta tattcagctt caattttgtc tctctctaaa cctgttagac
    15961 gtcttaaacg catgtctaaa atagcttgag cttgtttttc agaaagtttg aagcgttgtt
    16021 gcaagctttc cattgcaact ttatctgtat ctgactcacg aatcgttgaa ataatttcat
    16081 cgatatggtc aagtgcgata cgtaatcctt ctaaaatgtg ggcacgatct ttagctttac
    16141 gtaagttgta ttgcgtacgt cttctaacaa ctgtcttttg atgctctaaa taatgtacca
    16201 acgcttcttt taaattaata agcttcggtc taccatttac aagtgcaatc atattcacac
    16261 caaatgatgt ttgaagaggt gtttgtttgt ataagttatt taaaatgaca ctagcatttg
    16321 catccttacg cacatcaata acgacacgca caccagtacg taaacttgtt tcatcacgta
    16381 aatcagtgat accgtcaatt ttcttgtcac gaacgagctc tgcaattttt tcaatcatac
    16441 gagccttatt cacttggaaa ggaatttcag tgacaacaat acgttgacgt ccgcctccac
    16501 gttcttcaat aactgcacga gaacgcattt gaattgaacc acgacctgtt tcatatgcac
    16561 gtctaatacc actcttacct aaaataagtc cagcagttgg gaaatcagga ccttcaatat
    16621 cctccattaa ctcagcaatt gaaatatcag ggttcttact taagctaagt acaccattga
    16681 ttaattctgt taagttatgt ggtggaatat tcgttgccat acctaccgcg atacctgatg
    16741 caccattggc taataagtta gggaatcgag caggtaagac tgacggctct ctttcattac
    16801 catcatagtt atcgataaaa tctattgtat ctttattaat atcacgtaac agttcaagtg
    16861 tgattttagt catacgcgct tcagtataac gcattgctgc tgcgccatct ccatccattg
    16921 aaccaaagtt accttggcca tcaacaagcg gataacgata actgaaatct tgagccatac
    16981 gtaccattgc ttcataaata gataagtcac catgagggtg atatttaccc attacgtcac
    17041 caacgatacg tgctgatttt ttatatgatt tatccggtgt cataccttgt tcatttaatc
    17101 catatagtat acgacgatgt actggtttta aaccgtcacg aacatctggc aatgcacgag
    17161 caacgataac actcatcgca taatctaaaa atgattcacg catttcactg gtaatatttc
    17221 gttcatttat tcttgattga ggtaattcag ccatcaagag ttcctccttc aaaagttcag
    17281 ttcacagcgc ttagaagtct aagtttgcat aaactgcatt atcttctata aattgtctac
    17341 ggttttctac aacgtcaccc attaacattt caaatgtttg gtccgcttca atcgcatctt
    17401 caagttttac ttgtaaaaga gcgcggtgct cagggttcat tgttgtttcc cataattgat
    17461 ctgcattcat ttctccaaga cctttgtatc gtgcaataga ccattttggt gttggattca
    17521 attcagattt aagtttatca agttccctat cattgtatac ataatacttt tgtttacctt
    17581 gtgtcagttt atacaacggt ggctgtgcaa tatacacata gcctgcttca attaacggtc
    17641 tcataaatcg atagaagaat gttaataaca atgttctaat atgcgctcca tccacatcgg
    17701 catcagtcat aatgacgatt ttgtgatatc ttgctttcgc tagatcaaag tcgccaccga
    17761 ttcctgtacc aaatgctgtg atcatttgac gaatttcatt gttattcaaa attctatcta
    17821 atcgtgcttt ttcaacattt aatatcttac ctcgtaatgg taaaatcgcc tgcgttctag
    17881 agtcacgacc agattttgta gaccccccgg cagagtcccc ttcgactaag aaaatctcac
    17941 attcttcagg acttttacta gagcaatcgg ctaatttacc tggaaggctt gctacatcta
    18001 acgctgattt acgacgtgtt acttcacgcg cttttttcgc agcaacacgt gcacgtgccg
    18061 ccataatacc tttttcaacc actgtacgtg cgacttgtgg attttcatat aaaaatcgtt
    18121 caaagtgctc tgagaataat ttatctacaa cttgacgcac ttcagaatta cctaattttg
    18181 tcttcgtttg accttcgaat tgaggatcat catgtttgat agatataatt gctgtcatac
    18241 cttcacgtgt atcttcacca gaaagtctat ctttttcttc tttcataatc ttgctactta
    18301 aaccataact atttaagaca cgcgttaatg cacgtttgaa tccgtcttca tgcgtaccac
    18361 cttcatacgt atgaatgtta tttgcgtaag ttaaaagatt tgtggcatat cctgagttat
    18421 attgaatcgc aatttctact tcaatatcat ctttagattg atgaatataa attggctcat
    18481 catgaatagg ttctttattt tcgttcaata actcaacgta cgatttaata ccgccctcat
    18541 agtgatagga gtcttctcta acgttttctt catcacgttc atctcttaat gtgatttgaa
    18601 ttcctttgtt taagaaagca agctctctaa tacgctgctg taatgtttca tagttgtata
    18661 cagttgtctc tgtgaagatt tctccatctg ctttaaaacg aatgacagta cctgtcttat
    18721 cagttgtgcc aacttctttt aagtcaaatt gaggtacacc ttttttatat gcttgatgat
    18781 atatagtctc atttctgtgt acatatactt ctaagtcttg tgacaatgcg tttacaactg
    18841 atgaaccaac accatgtaaa ccaccagata ctttgtatcc gccaccgcca aatttaccac
    18901 cagcatgtaa aacagttaaa ataacttcga cagctggacg tcccattttt tcttgaatat
    18961 caactgggat accacgtccg ttatccgtta ctttaatcca gttatctttt tcaataacaa
    19021 cttcaatttg atttgcataa ccagctaatg cttcatcgat actattatcg acaatttccc
    19081 acactaaatg gtgcaaacct ctctctgaag tcgatcctat atacatacct ggtcttttac
    19141 gtactgcttc taaaccttct aatacttgta tttgcccagc accataatta tccgtgttgt
    19201 ttacatctga caatgcagtc accatcgctt tctgttactt tataatttca ccttgattaa
    19261 tacgatacaa tttagcgtta ttcatgattt catgatcaat accatctaca gatgtcgtag
    19321 tgacaaatgt ttgtacttta tgctgaatcg tacttaataa atgcgtttga cgcgaatcat
    19381 ctaattcact gagtacatcg tctaataata agatgggata ttccccaact tcgatattca
    19441 ttaactcaat ttcagctaat ttaatggaca aagccgttgt acgttgctgt ccttgagaac
    19501 catatgtttg agcatccatg ccattcacat caaaacttat atcatctcga tgtggtccga
    19561 ataagctaat gcctcgttct ttttctcttt gcatattatc gctaagaata gacataattt
    19621 cttcaagtcg tgccgcttca ttttgagcat aatcaaattt aagactaggt aaataattca
    19681 gcgacaacgc ttctttatca tttgtgatac cagcatgaat cggtttagct aacgactcta
    19741 gctcttgaat aaaatgtgca cgtttatcag ttactttcat tgcatattca gcaaactgct
    19801 gatttaatac ttccaacatt gttaagtcct ttttttggcc taattgtaac tgctttaagt
    19861 aattattctt ttgctttaaa atacgttggt attgagctaa atcatttaag taaacagcag
    19921 aaatttggcc caactccata tctataaagc gtcgtcttat ttgaggagag ccttttacaa
    19981 tattcaaatc ttctggcgca aatagaacca cattgaggtg tccaatatat tgagttagac
    20041 gactttgctc taagtgattc actttgactt gtttaccttt tttagttata aacattgtta
    20101 atggcatcgt gccgtgtcta taactaagct caccttctat tttagcataa tcagcattaa
    20161 aacgtatgag ttccttatca ttactcgttc tatgactttt tgctaaagct aaggtataaa
    20221 ttgattcaag taaatttgtc tttccttgtg cattttctcc aatgaggata ttcacgtcag
    20281 gatgacattt caacgtaacc tcatcatagt tacgataatt ttctaattgg agtgtattta
    20341 acttcattgt tcaccttgat gaatgattaa gaaagaacca gcatcttcag gtaattctgg
    20401 gatatctata cgatcttgat gttctaactt tttaccgcga cgtgtttcac gcactccatt
    20461 aattaatact tcaacgtctt gcaagaacca ttttgcttga ccaccagatt caataatccc
    20521 ttctgttttt agaaattgac ccaaattaat gtctccttct actacaactt cttgaaccaa
    20581 aataatcact ccatttcttt gtatgtcatt aatatattat accttttttt gtcacaaatt
    20641 tcatttaa
//
LOCUS       DAXWFK010000028        20508 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.028, whole genome shotgun sequence.
ACCESSION   DAXWFK010000028 DAXWFK010000000
VERSION     DAXWFK010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20508
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.028"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            339..647
                     /gene="rpsJ"
                     /locus_tag="ACGEXS_000821"
     CDS             339..647
                     /gene="rpsJ"
                     /locus_tag="ACGEXS_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFF8011855.1"
                     /translation="MAKQKIRIRLKAYDHRVIDQSAEKIVETAKRSGADVSGPIPLPT
                     EKSVYTIIRAVHKYKDSREQFEQRTHKRLIDIVNPTPKTVDALMGLNLPSGVDIEIKL
                     "
     gene            675..1337
                     /gene="rplC"
                     /locus_tag="ACGEXS_000822"
     CDS             675..1337
                     /gene="rplC"
                     /locus_tag="ACGEXS_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFF8011856.1"
                     /translation="MTKGILGRKIGMTQVFGENGELIPVTVVEAKENVVLQKKTVEVD
                     GYNAIQVGFEDKKAYKKDAKSNKYANKPAEGHAKKADAAPKRFIREFRNVDVDAYEVG
                     QEVSVDTFVAGDVIDVTGVSKGKGFQGAIKRHGQSRGPMSHGSHFHRAPGSVGMASDA
                     SRVFKGQKMPGRMGGNTVTVQNLEVVQVDTENKVILVKGNVPGPKKGLVEIRTSIKKG
                     NK"
     gene            1364..1987
                     /gene="rplD"
                     /locus_tag="ACGEXS_000823"
     CDS             1364..1987
                     /gene="rplD"
                     /locus_tag="ACGEXS_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFF8011857.1"
                     /translation="MANYDVLKLDGTKSGSIELSDAVFGIEPNNSVLFEAINLQRASL
                     RQGTHAVKNRSAVSGGGRKPWKQKGTGRARQGTIRAPQWRGGGIVFGPTPRSYAYKMP
                     KKMRRLALRSALSFKAQENGLTVVDAFNFEAPKTKEFKNVLSTLEQPKKVLVVTENED
                     VNVELSARNIPGVQVTTAQGLNVLDITNADSLVITEAAAKKVEEVLE"
     gene            1987..2262
                     /gene="rplW"
                     /locus_tag="ACGEXS_000824"
     CDS             1987..2262
                     /gene="rplW"
                     /locus_tag="ACGEXS_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002506966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFF8011858.1"
                     /translation="MEARDILKRPVITEKSSEAMAEDKYTFDVDTRVNKTQVKMAVEE
                     IFNVKVASVNIMNYKPKKKRMGRYQGYTNKRRKAIVTLKEGSIDLFN"
     gene            2295..3128
                     /gene="rplB"
                     /locus_tag="ACGEXS_000825"
     CDS             2295..3128
                     /gene="rplB"
                     /locus_tag="ACGEXS_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFF8011859.1"
                     /translation="MAIKKYKPITNGRRNMTSLDFAEITKTTPEKSLLKPLPKKAGRN
                     NQGKLTVRHHGGGHKRQYRVIDFKRNKDGINAKVDSIQYDPNRSANIALVVYADGEKR
                     YIIAPKGLEVGQIVESGAEADIKVGNALPLQNIPVGTVVHNIELKPGKGGQIARSAGA
                     SAQVLGKEGKYVLIRLRSGEVRMILSTCRATIGQVGNLQHELVNVGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGRAPIGRPSPMSPWGKPTLGKKTRRGKKSSDKLIVRGRKK
                     K"
     gene            3195..3473
                     /gene="rpsS"
                     /locus_tag="ACGEXS_000826"
     CDS             3195..3473
                     /gene="rpsS"
                     /locus_tag="ACGEXS_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFF8011860.1"
                     /translation="MARSIKKGPFVDEHLMKKVEAQEGSEKKQVIKTWSRRSTIFPNF
                     IGHTFAVYDGRKHVPVYVTEDMVGHKLGEFAPTRTFKGHVADDKKTRR"
     gene            3502..3855
                     /gene="rplV"
                     /locus_tag="ACGEXS_000827"
     CDS             3502..3855
                     /gene="rplV"
                     /locus_tag="ACGEXS_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFF8011861.1"
                     /translation="MEAKAVARTIRIAPRKVRLVLDLIRGKNAAEAIAILKLTNKASS
                     PVIEKVLMSALANAEHNYDMNTDELVVKEAYANEGPTLKRFRPRAQGRASAINKRTSH
                     ITIVVSDGKEEAKEA"
     gene            3879..4532
                     /gene="rpsC"
                     /locus_tag="ACGEXS_000828"
     CDS             3879..4532
                     /gene="rpsC"
                     /locus_tag="ACGEXS_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFF8011862.1"
                     /translation="MGQKINPIGLRVGIIRDWEAKWYAEKDFASLLHEDLKIRKFIDN
                     ELKEASVSHVEIERAANRINIAIHTGKPGMVIGKGGSEIEKLRNKLNALTDKKVHINV
                     IEIKKVDLDARLVAENIARQLENRASFRRVQKQAITRAMKLGAKGIKTQVSGRLGGAD
                     IARAEQYSEGTVPLHTLRADIDYAHAEADTTYGKLGVKVWIYRGEVLPTKNTSGGGK"
     gene            4535..4969
                     /gene="rplP"
                     /locus_tag="ACGEXS_000829"
     CDS             4535..4969
                     /gene="rplP"
                     /locus_tag="ACGEXS_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFF8011863.1"
                     /translation="MLLPKRVKYRRQHRPKTTGRSKGGNYVTFGEFGLQATTTSWITS
                     RQIESARIAMTRYMKRGGKVWIKIFPHTPYTKKPLEVRMGAGKGAVEGWIAVVKPGRI
                     LFEVAGVSEEVAREALRLASHKLPVKTKFVKREELGGETNES"
     gene            4959..5168
                     /gene="rpmC"
                     /locus_tag="ACGEXS_000830"
     CDS             4959..5168
                     /gene="rpmC"
                     /locus_tag="ACGEXS_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002509269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFF8011864.1"
                     /translation="MKAKEIRDLTTSEIEEQIKSSKEELFNLRFQLATGQLEETARIR
                     TVRKTIARLKTVAREREIEQSKANQ"
     gene            5192..5455
                     /gene="rpsQ"
                     /locus_tag="ACGEXS_000831"
     CDS             5192..5455
                     /gene="rpsQ"
                     /locus_tag="ACGEXS_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFF8011865.1"
                     /translation="MSERNDRKVYVGKVVSDKMDKTITVLVETYKTHKLYGKRVKYSK
                     KYKTHDENNSAKLGDIVKIQETRPLSATKRFRLVEIVEESVII"
     gene            5487..5855
                     /gene="rplN"
                     /locus_tag="ACGEXS_000832"
     CDS             5487..5855
                     /gene="rplN"
                     /locus_tag="ACGEXS_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFF8011866.1"
                     /translation="MIQQETRLKVADNSGAREVLTIKVLGGSGRKTANIGDVIVCTVK
                     NATPGGVVKKGDVVKAVIVRTKSGVRRNDGSYIKFDENACVIIRDDKGPRGTRIFGPV
                     ARELREGNFMKIVSLAPEVL"
     gene            5891..6208
                     /gene="rplX"
                     /locus_tag="ACGEXS_000833"
     CDS             5891..6208
                     /gene="rplX"
                     /locus_tag="ACGEXS_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFF8011867.1"
                     /translation="MHIKKGDNVKVIAGKDKGKEGKVIATLPKKDRVVVEGVNIMKKH
                     QKPTQLNPEGGILETEAAIHVSNVQLLDPKTNEPTRVGYKFVDGKKVRIAKKSGEEIK
                     SNN"
     gene            6235..6774
                     /gene="rplE"
                     /locus_tag="ACGEXS_000834"
     CDS             6235..6774
                     /gene="rplE"
                     /locus_tag="ACGEXS_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFF8011868.1"
                     /translation="MNRLKEKFNTEVTENLMKKFNYSSVMEVPKIDKIVVNMGVGDAV
                     QNSKVLDNAVEELELITGQKPLVTKAKKSIATFRLREGMPIGAKVTLRGERMYEFLDK
                     LISVSLPRVRDFQGVSKKAFDGRGNYTLGVKEQLIFPEIDYDKVSKVRGMDIVIVTTA
                     NTDEEARELLANFGMPFRK"
     gene            6797..6982
                     /locus_tag="ACGEXS_000835"
     CDS             6797..6982
                     /locus_tag="ACGEXS_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFF8011869.1"
                     /translation="MAKTSMVAKQQKKQKYAVREYTRCERCGRPHSVYRKFKLCRICF
                     RELAYKGQIPGVRKASW"
     gene            7014..7412
                     /gene="rpsH"
                     /locus_tag="ACGEXS_000836"
     CDS             7014..7412
                     /gene="rpsH"
                     /locus_tag="ACGEXS_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFF8011870.1"
                     /translation="MTMTDPIADMLTRVRNANMVRHEKLELPASNIKKEIAEILKSEG
                     FIKNVEYVEDDKQGVLRLFLKYGQNDERVITGLKRISKPGLRVYAKASEMPKVLNGLG
                     IALVSTSEGVITDKEARKRNVGGEIIAYVW"
     gene            7437..7973
                     /gene="rplF"
                     /locus_tag="ACGEXS_000837"
     CDS             7437..7973
                     /gene="rplF"
                     /locus_tag="ACGEXS_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFF8011871.1"
                     /translation="MSRVGKKIIDIPSDVTVTFDGNHVTVKGPKGELSRTLNERMTFK
                     QEENTIEVVRPSDSKEDRTNHGTTRALLNNMVQGVSQGYVKVLELVGVGYRAQMQGKD
                     LILNVGYSHPVEIKAEENITFSVEKNTVVKVEGISKEQVGALASNIRSVRPPEPYKGK
                     GIRYQGEYVRRKEGKTGK"
     gene            8004..8363
                     /gene="rplR"
                     /locus_tag="ACGEXS_000838"
     CDS             8004..8363
                     /gene="rplR"
                     /locus_tag="ACGEXS_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFF8011872.1"
                     /translation="MISKIDKNKVRLKRHARVRTNLSGTAEKPRLNVYRSNKHIYAQI
                     IDDNKGVTLAQASSKDSDIATTATKVELATKVGEAIAKKAADKGIKEIVFDRGGYLYH
                     GRVKALAEAARESGLEF"
     gene            8384..8884
                     /gene="rpsE"
                     /locus_tag="ACGEXS_000839"
     CDS             8384..8884
                     /gene="rpsE"
                     /locus_tag="ACGEXS_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFF8011873.1"
                     /translation="MARREEETKEFEERVVTINRVAKVVKGGRRFRFTALVVVGDKNG
                     RVGFGTGKAQEVPEAIKKAVEAAKKDLVVVPRVEGTTPHTITGRYGSGSVFMKPAAPG
                     TGVIAGGPVRAVLELAGITDILSKSLGSNTPINMVRATIDGLQNLKNAEDVAKLRGKT
                     VEELYN"
     gene            8901..9080
                     /gene="rpmD"
                     /locus_tag="ACGEXS_000840"
     CDS             8901..9080
                     /gene="rpmD"
                     /locus_tag="ACGEXS_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFF8011874.1"
                     /translation="MAKLQITLTRSVIGRPETQRKTVEALGLKKTNSSVVVEDNPAIR
                     GQINKVKHLVTVEEK"
     gene            9097..9537
                     /gene="rplO"
                     /locus_tag="ACGEXS_000841"
     CDS             9097..9537
                     /gene="rplO"
                     /locus_tag="ACGEXS_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFF8011875.1"
                     /translation="MKLHELKPAEGSRKERNRVGRGVATGNGKTSGRGHKGQKARSGG
                     GVRPGFEGGQLPLFRRLPKRGFTNINRKEYAIVNLDQLNKFEDGTEVTPALLVESGVV
                     KNEKSGIKILGNGSLDKKLTVKAHKFSASAAEAIDAKGGAHEVI"
     gene            9537..10829
                     /gene="secY"
                     /locus_tag="ACGEXS_000842"
     CDS             9537..10829
                     /gene="secY"
                     /locus_tag="ACGEXS_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFF8011876.1"
                     /translation="MIQTLVNFFRTKEVRNKIFFTLAMLVIFKIGTYIPAPGVNPAAF
                     DNPQGSQGATELLNTFGGGALKRFSIFAMGIVPYITASIVMQLLQMDIVPKFSEWAKQ
                     GEVGRRKLNNVTRYLAISLAFIQSIGMAFQFNNYLKGALIINQSIMSYLLIALVLTAG
                     TAFLIWLGDQITQFGVGNGISIIIFAGILSTLPASLIQFGQTAFVGQEDTSLAWLKVL
                     GLLVSLILLTVGAIYVLEAVRKIPIQYAKKQTAQRLGSQATYLPLKVNSAGVIPVIFA
                     MAFFLLPRTLTLFYPDKEWAQNIANAANPSSNVGMVVYIVLIILFTYFYAFVQVNPEK
                     MADNLKKQGSYVPGIRPGEQTKKYITKVLYRLTFVGSIFLAVISILPILATKFMGLPQ
                     SIQIGGTSLLIVIGVAIETMKSLEAQVSQKEYKGFGGR"
     gene            10846..11493
                     /locus_tag="ACGEXS_000843"
     CDS             10846..11493
                     /locus_tag="ACGEXS_000843"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFF8011877.1"
                     /translation="MNIILMGLPGAGKGTQASEIVKKFPIPHISTGDMFRKAIKEETE
                     LGKEAKSYMDRGELVPDEVTVGIVKERISEDDAKKGFLLDGFPRTIEQAEALNNIMSE
                     LDRNIDAVINIEVPEEELMNRLTGRRICESCGTTYHLVFNPPKVEGICDIDGGKLYQR
                     EDDNPETVANRLSVNIKQSKPILDFYDQKGVLKNIDGSKDISDVTKDVIDILDHL"
     gene            11686..11904
                     /gene="infA"
                     /locus_tag="ACGEXS_000844"
     CDS             11686..11904
                     /gene="infA"
                     /locus_tag="ACGEXS_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFF8011878.1"
                     /translation="MAKQDVIELEGTVLDTLPNAMFKVELENGHEILAHVSGKIRMNY
                     IRILPGDKVTVEMSPYDLTRGRITYRYK"
     gene            11936..12049
                     /gene="rpmJ"
                     /locus_tag="ACGEXS_000845"
     CDS             11936..12049
                     /gene="rpmJ"
                     /locus_tag="ACGEXS_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFF8011879.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQRQG"
     gene            12072..12437
                     /gene="rpsM"
                     /locus_tag="ACGEXS_000846"
     CDS             12072..12437
                     /gene="rpsM"
                     /locus_tag="ACGEXS_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFF8011880.1"
                     /translation="MARIAGVDIPREKRVVISLTYIYGIGTSTAQKILEEANVSADTR
                     VKDLTDDELGRIREVVDGYKVEGDLRRETNLNIKRLMEISSYRGIRHRRGLPVRGQKT
                     KNNARTRKGPVKTVANKKK"
     gene            12461..12850
                     /gene="rpsK"
                     /locus_tag="ACGEXS_000847"
     CDS             12461..12850
                     /gene="rpsK"
                     /locus_tag="ACGEXS_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFF8011881.1"
                     /translation="MARKQVSRKRRVKKNIENGVAHIRSTFNNTIVTITDEFGNALSW
                     SSAGALGFKGSKKSTPFAAQMASETASKSAMEHGLKTVEVTVKGPGPGRESAIRALQS
                     AGLEVTAIRDVTPVPHNGCRPPKRRRV"
     gene            12925..13869
                     /locus_tag="ACGEXS_000848"
     CDS             12925..13869
                     /locus_tag="ACGEXS_000848"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFF8011882.1"
                     /translation="MIEIEKPRIETIEISEDAKFGKFVVEPLERGYGTTLGNSLRRIL
                     LSSLPGAAVKYIEIEGVLHEFSAVDNVVEDVSTIIMNIKQLALKIYSEEDKTLEIDVR
                     DEGEVTASDITHDSDVEILNPELKIATVSKGGHLKIRLVANKGRGYALAEQNNTSDLP
                     IGVIPVDSLYSPVERVNYTVENTRVGQSSDFDKLTLDVWTNGSITPQESVSLAAKIMT
                     EHLNIFVGLTDEAQNAEIMIEKEEDQKEKVLEMSIEELDLSVRSYNCLKRAGINSVQE
                     LADKSEADMMKVRNLGRKSLEEVKYKLEDLGLGLRKED"
     gene            13886..14254
                     /gene="rplQ"
                     /locus_tag="ACGEXS_000849"
     CDS             13886..14254
                     /gene="rplQ"
                     /locus_tag="ACGEXS_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002450507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFF8011883.1"
                     /translation="MGYRKLGRTSDQRKAMLRDLATSLIISERIETTEARAKEVRSVV
                     EKLITLGKKGDLASRRNAAKTLRNVEILNEDETTQTALQKLFGEIAERYTERQGGYTR
                     ILKQGPRRGDGAESVIIELV"
     gene            14792..15601
                     /locus_tag="ACGEXS_000850"
     CDS             14792..15601
                     /locus_tag="ACGEXS_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HFF8011884.1"
                     /translation="MEDKNSVIVFKNVSFQYQSDASFTLKDVSFNIPKGQWTSIVGHN
                     GSGKSTIAKLMIGIEKVKSGEIFYNNQAITDDNFEKLRKDIGIVFQNPDNQFVGSIVK
                     YDVAFGLENHAVPYDEMHRRVSEALKQVDMLERADYEPNALSGGQKQRVAIASVLALN
                     PSVIILDEATSMLDPDARQNLLDLVRKVKSEHNITIISITHDLSEAMEADHVIVMNKG
                     TVYKEGTATEIFDNAEELTRIGLDLPFPIKINQMLGHQTSFLTYEGLVDQL"
     gene            15598..16458
                     /locus_tag="ACGEXS_000851"
     CDS             15598..16458
                     /locus_tag="ACGEXS_000851"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HFF8011885.1"
                     /translation="MTIRFDNVSYTYQKGTPYQHQAIHDVNTEFEQGKYYAIVGQTGS
                     GKSTLIQNINALLKPTTGTVTVDDITITHKTKDKYIRPVRKRIGMVFQFPESQLFEDT
                     VEREMIFGPKNFKMNLDEAKNYAHRLLMDLGFSRDVMSQSPFQMSGGQMRKIAIVSIL
                     AMNPDIIVVDEPTAGLDPQSKRQVMRLLKSLQTDENKAIILISHDMNEVARYADEVIV
                     MKEGSIVSQTSPKELFKDKKKLADWHIGLPEIVQLQYDFEQKYQTKLKDIALTEEAFV
                     SLYKEWQHEK"
     gene            16448..17254
                     /locus_tag="ACGEXS_000852"
     CDS             16448..17254
                     /locus_tag="ACGEXS_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFF8011886.1"
                     /translation="MKNKLIIGRYLPINSFVHHLDPRAKLMFVFLFIILIFFCHSPLT
                     YLWVFALILFIMRLAKIQLWFLIKGLTPIFFFLIFTLMMHIFLTKGGYVLVEWHGITI
                     ETNGILEGLYISLRLIGIVMIATIMTLSTSPIDLTDAFERLLAPLKMFKLPVHQLSMI
                     MSIALRFIPTLMDELDKIILAQKSRGSEISSGNIATRIKSFIPLLVPLFISAFQRAEE
                     LAVAMEVRGYDANVKRTSYRQLKWQLRDTISLTMIIPIAIILFVLKYSGV"
     gene            17259..18062
                     /gene="truA"
                     /locus_tag="ACGEXS_000853"
     CDS             17259..18062
                     /gene="truA"
                     /locus_tag="ACGEXS_000853"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFF8011887.1"
                     /translation="MRILVEIAYQGNNFLGFQIQQNGRTVQQQFEKLLQRMHKRHVRI
                     HPSSRTDRGVHAIQQYFHFDTELNIPMSQWQYAMNRTLPDDIYVNNVVTVDDDFHCRY
                     DCVGKRYRYKVYQAQHRDPFQSGLKTFIPETLDLGKMNRAAQQFIGTHDFTGFCSQKT
                     EVESKVRTLYQSEIVKTDDGFDYIVTGSGFLYNMVRVLVAFLIEVGKGRHEVSDVPKL
                     LESKNRKNVPFTAPAEGLYLEKIYLDENELLKDFGNDIKIHRKKSLQND"
     gene            18302..18739
                     /gene="rplM"
                     /locus_tag="ACGEXS_000854"
     CDS             18302..18739
                     /gene="rplM"
                     /locus_tag="ACGEXS_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFF8011888.1"
                     /translation="MRQTFMANESNIERKWYVIDAEGQTLGRLSSEVASILRGKNKVT
                     YTPHVDTGDYVIVINASKIEFTGNKETDKVYYRHSNHPGGIKSITAGELRRTNPERLI
                     ENSIKGMLPSTRLGEKQGKKLFVYGGAEHPHAAQQPENYELRG"
     gene            18759..19151
                     /gene="rpsI"
                     /locus_tag="ACGEXS_000855"
     CDS             18759..19151
                     /gene="rpsI"
                     /locus_tag="ACGEXS_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001790547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFF8011889.1"
                     /translation="MAQVEYRGTGRRKNSVARVRLVPGEGNITVNNRDVREYLPFESL
                     ILDLNQPFDVTETKGNYDVLVNVHGGGFTGQAQAIRHGIARALLEADPEYRGSLKRAG
                     LLTRDPRMKERKKPGLKAARRSPQFSKR"
     gene            19696..>20508
                     /locus_tag="ACGEXS_000856"
     CDS             19696..>20508
                     /locus_tag="ACGEXS_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type ISP restriction/modification enzyme"
                     /protein_id="HFF8011890.1"
                     /translation="MRGDQRTQGEKSRKEGGKIFGSGSRAPIAISILVKDGSYNHDIY
                     YNDIGEYLTREQKLDTLMKHQSIVNLKSLNVLPDKNNDWINQRDINYENYLPMYDSKD
                     IENSIYLDQFNGVNSARDNWVTNFSNEKALVNAKLLVDNYNSEIDRLIDILDSRERIN
                     LVNKDETFISWTRGLTQKFSKGKNISINPERIVKFMHRPFTKKWIVYDKNIMEMPSRY
                     YNIMENTGQVIYIQGQGMNKEFSAMITDILPNFQFIGNGKGFATYKGKDSLRL"
BASE COUNT         7473 a         3214 c         4024 g         5797 t
ORIGIN      
        1 ttatgactaa ttatatagaa taattttcaa tgataataat aaaatgattc aaggtacgaa
       61 tttacaataa agaaggaaat aaaaaatatc tcgaaaatag ttgaactgac taagtatata
      121 aagtaaaata taaaagtatg tgttagacag atagaaaaat tatgtgaaaa gccttgattt
      181 atcgatgttt ttcgtgtaat ataactaacg ttggacttta agaaaagcga tgaagcgaaa
      241 ggttactgac acacccggcc gctttgccat ggcgctgtgt aagatagttt tcgtggagaa
      301 gtctatcact aaatgtagac gaataaggag ggaaaattat ggcaaaacaa aaaatcagaa
      361 tcagattaaa agcttatgat caccgcgtaa ttgatcaatc agcagagaag attgtagaaa
      421 cagcgaaacg ttctggtgca gatgtttctg gaccaattcc gttaccaact gagaaatcag
      481 tttacacaat catccgtgcc gtgcataagt ataaagattc acgtgaacaa ttcgaacaac
      541 gtacacacaa acgtttaatc gatattgtaa acccaacacc aaaaacagtt gacgctttaa
      601 tgggcttaaa cttaccatct ggtgtagaca tcgaaatcaa attataatag acaattttag
      661 gaggtggact ttcgatgacc aaaggaatct taggaagaaa aattgggatg acacaagtat
      721 tcggagaaaa cggtgaatta atccctgtaa cagtagtaga agctaaagaa aatgttgtat
      781 tacaaaagaa aactgtagaa gttgatggat acaacgcaat ccaagttgga tttgaagaca
      841 aaaaagcata caaaaaagat gcaaaatcta ataaatatgc taataaacca gctgaaggtc
      901 acgctaaaaa agctgacgca gcacctaagc gcttcattcg tgaattccgc aatgtagacg
      961 tggatgctta cgaagtaggt caagaagtct cagtagatac ttttgtagct ggcgacgtta
     1021 ttgacgtaac aggcgtatca aaaggtaaag gtttccaagg tgcaattaaa cgccacggac
     1081 aatctcgtgg acctatgtca cacggttctc atttccacag agcaccaggt tctgtaggta
     1141 tggcttcaga tgcttctaga gtatttaaag gccaaaaaat gccaggacgt atgggtggaa
     1201 acactgtaac tgttcaaaac ttagaagtag ttcaagttga cacagaaaac aaagttatct
     1261 tagtaaaagg taacgtacct ggacctaaaa aaggtttagt agaaatcaga acttcaatta
     1321 aaaaaggtaa taaataataa gaagtgaaag gaggaaatgc ataatggcta attatgatgt
     1381 tttaaaatta gacggaacta aatcaggttc aatcgaatta agcgatgcag tattcggtat
     1441 tgagccaaat aatagcgttt tattcgaagc tattaattta caacgtgctt cattacgtca
     1501 aggtacgcat gctgttaaga atcgttcagc agtaagcggt ggcggacgta aaccatggaa
     1561 gcaaaaagga acaggtcgtg ctcgtcaagg tacaatccgt gctccacaat ggcgtggcgg
     1621 tggtatcgta ttcggaccaa ctccaagaag ttatgcatac aaaatgccta agaaaatgcg
     1681 tcgtttagct ttacgctcag cattatcttt caaagctcaa gagaatggct taactgtagt
     1741 tgacgcattc aacttcgaag ctccaaaaac taaagaattc aaaaatgtat tatctacatt
     1801 agaacaacct aaaaaagtat tagtagttac tgaaaacgaa gatgtaaatg ttgaattatc
     1861 agcacgcaac atccctggcg ttcaagtgac aactgctcaa ggtttaaatg ttttagatat
     1921 cactaatgct gacagcttag taattactga agctgctgct aaaaaagttg aggaggtgct
     1981 cgaataatgg aagcaagaga tattcttaag cgccccgtaa tcactgagaa atcttctgaa
     2041 gcaatggctg aagacaaata cactttcgac gttgatactc gcgttaacaa aacacaagta
     2101 aaaatggcag ttgaagaaat cttcaacgta aaagttgcaa gtgttaatat catgaattac
     2161 aaacctaaga aaaaacgtat gggccgttac caaggctata caaacaaaag aagaaaagcg
     2221 attgtaactc ttaaagaagg atcaatcgac ttatttaact aataaaacaa ttaccattaa
     2281 ggaggtaagc gacaatggct attaaaaagt ataagccaat aacaaatggt cgtcgtaata
     2341 tgacttcgtt agatttcgca gaaatcacga aaactacacc tgaaaagtca ttattaaaac
     2401 cgctaccgaa aaaagcggga cgtaacaacc aaggtaaatt gactgtaaga caccatggtg
     2461 gtggacacaa acgtcaatac cgtgttatcg atttcaaacg taacaaagat ggtatcaatg
     2521 caaaagttga ttctattcaa tatgatccaa accgctcagc aaacatcgct ttagttgtat
     2581 atgcagacgg tgaaaaacga tatatcattg ctcctaaagg attagaagta ggtcaaatcg
     2641 ttgaaagtgg tgctgaagct gacatcaaag ttggtaacgc attaccatta caaaacattc
     2701 cagttggtac agtagtacac aacatcgagc ttaaacctgg taaaggtgga caaatcgctc
     2761 gttcagctgg tgcaagtgct caagtacttg gtaaagaagg taaatacgta ttaatcagat
     2821 taagatctgg tgaagttcgt atgatcttat ctacttgccg tgctacaatc ggtcaagttg
     2881 gtaacctaca acacgaatta gttaacgttg gtaaagccgg acgttcaaga tggaaaggta
     2941 tccgtccaac agttcgtggt tctgtaatga accctaacga tcacccacac ggtggtggtg
     3001 aaggtcgtgc tcctatcggt agaccatctc caatgtcacc atggggtaaa cctacgcttg
     3061 gtaagaaaac tcgtcgtggt aaaaaatcat cagacaaact tatcgttcgt ggacgtaaga
     3121 aaaaataata taatcaactt atttgggtgt gcggcttaaa gctgcacgca cataataaga
     3181 agggaggcgc ccaaatggct cgtagtatta aaaaaggacc tttcgtcgat gagcatttaa
     3241 tgaaaaaagt tgaagctcaa gaaggaagcg aaaagaaaca agtaatcaaa acatggtcac
     3301 gtcgttctac aattttccct aatttcatcg gacatacttt tgcagtatac gacggacgta
     3361 aacacgtacc tgtatatgta actgaagata tggtaggtca taaattaggt gagtttgctc
     3421 ctactcgtac attcaaagga cacgttgcag acgacaagaa aacaagaaga taatatctat
     3481 taagtagagg aggacatcct aatggaagca aaagcggttg ctagaacaat aagaatcgca
     3541 cctcgtaaag taagactagt tcttgactta atcagaggta aaaatgctgc tgaagctatt
     3601 gcaattttaa aattaacaaa caaagcttca tcaccagtaa ttgaaaaagt attaatgtcc
     3661 gctttagcta atgctgaaca taactatgac atgaacacag atgaattagt agttaaagaa
     3721 gcatatgcta acgaaggacc aacattaaaa cgtttccgtc cacgtgcgca aggtcgtgca
     3781 agtgcgatta acaaacgtac aagccacatt acaatcgtcg taagtgacgg taaagaagaa
     3841 gctaaagaag cttaattaac ttttaaggag ggaatactgt gggtcaaaaa attaatccaa
     3901 tcggacttcg tgttggtatt atccgtgatt gggaagctaa atggtatgct gaaaaagact
     3961 tcgcttcact tttacacgaa gatttaaaaa tccgtaaatt tattgataat gaattaaaag
     4021 aagcatcagt ttctcacgta gagattgaac gtgctgcaaa ccgtatcaac attgcaattc
     4081 atactggtaa acctggtatg gtaattggta aaggcggttc agaaatcgaa aaattacgca
     4141 acaaattaaa tgcgttaact gataaaaaag tacacatcaa cgtaattgaa atcaaaaaag
     4201 ttgatcttga cgctcgttta gtagctgaaa acatcgcacg tcaattagaa aaccgtgctt
     4261 cattccgtcg tgtacaaaaa caagcaatca ctagagctat gaaacttggt gctaaaggta
     4321 tcaaaactca agtatctggt cgtttaggcg gagctgacat cgctcgtgct gaacaatatt
     4381 cagaaggaac tgttccactt catacgttac gtgctgacat cgattatgca cacgctgaag
     4441 ctgacactac ttacggtaaa ttaggcgtta aagtatggat ttatcgtgga gaagttcttc
     4501 ctactaagaa cactagtgga ggaggaaaat aataatgtta ctaccaaaac gtgtaaaata
     4561 tcgtcgtcaa catcgtccta aaacaactgg tcgttctaaa ggcggtaact acgtaacatt
     4621 tggtgagttt ggtttacaag ctacaacaac gtcttggatc acatctcgtc aaatcgaatc
     4681 tgctcgtata gcaatgacac gttacatgaa acgtggcggg aaagtttgga ttaaaatctt
     4741 cccacataca ccatatacta aaaaaccttt agaagtacgt atgggtgctg gtaaaggtgc
     4801 ggttgaaggc tggatcgcag ttgttaaacc aggtagaatt ttattcgaag ttgctggcgt
     4861 ttctgaagaa gttgcgcgtg aagcactacg tttagcaagt cacaaacttc cagtaaaaac
     4921 taagtttgta aaacgtgagg aattgggtgg tgaaacaaat gaaagctaag gaaattagag
     4981 acttaaccac ttcagaaatc gaagaacaaa tcaaatcttc aaaagaagag ctttttaacc
     5041 tacgctttca gttagctaca ggtcaattag aagaaactgc acgtattcgt acagtaagaa
     5101 aaacgattgc acgtctaaaa actgttgctc gtgaaagaga aattgaacaa agtaaggcta
     5161 atcaataatt aagtaagagg aggttacaaa agtgagcgaa agaaacgatc gtaaagttta
     5221 tgtaggtaaa gttgtttcag acaaaatgga caagactatt acagtacttg ttgaaactta
     5281 caaaacacac aaattatacg gtaaacgagt aaaatactct aaaaaataca aaactcatga
     5341 tgaaaacaat tcagctaaat taggagacat tgttaaaatt caagaaactc gtcctttatc
     5401 agcaacaaaa cgttttcgtt tagtagagat tgttgaagag tcagtaatta tttaatacaa
     5461 gtttagagat aagggaggtt taactaatga tccaacaaga aacacgcttg aaagtagcag
     5521 acaactctgg tgctcgtgaa gttcttacaa tcaaagtatt aggtggatct ggtcgtaaaa
     5581 cagcaaacat cggcgatgtt atcgtatgta ctgttaaaaa tgcaacacca ggtggcgttg
     5641 ttaaaaaagg tgacgttgtc aaagctgtaa tcgtacgtac taagtcaggt gttcgtcgta
     5701 atgacggttc atacatcaaa tttgatgaaa atgcatgtgt tatcatccgt gatgacaaag
     5761 gcccacgtgg tactcgtatc ttcggacctg ttgctcgtga attacgtgaa ggtaacttca
     5821 tgaaaatcgt atcattagca ccagaagtac tttaattaat aacaaccaat acaaataagg
     5881 aggtgcccac atgcatatca aaaaaggtga caacgttaaa gttatcgcag gtaaagacaa
     5941 aggtaaagaa ggtaaagtaa ttgctactct acctaaaaaa gaccgtgtcg ttgtggaagg
     6001 tgttaacatt atgaaaaaac accaaaaacc aactcaatta aatcctgaag gtggaatctt
     6061 agaaacagag gcagcaatcc atgtttctaa tgtacaatta ttggacccta aaacaaacga
     6121 accaactcgt gtaggttaca aatttgttga tggtaaaaaa gttcgtatcg ctaaaaaatc
     6181 tggcgaagaa attaaatcta ataattaata acaagttgaa aggaggatcc cactttgaac
     6241 cgtttaaaag aaaagtttaa cactgaagtt actgaaaact taatgaaaaa attcaattat
     6301 agttcagtaa tggaagtacc aaaaatagat aaaatcgttg tgaacatggg tgtaggtgac
     6361 gcagtacaaa attctaaagt attagacaat gctgttgaag aattagaatt gatcactggt
     6421 caaaaaccat tagtaactaa agctaaaaaa tcaatcgcga ctttccgttt acgtgaaggt
     6481 atgccaatcg gtgcgaaagt aacacttcgc ggtgaaagaa tgtatgaatt cttagacaaa
     6541 ttaatttcag tatcattacc acgtgtacgt gacttccaag gtgtttctaa aaaagcattt
     6601 gacggacgcg gtaactacac tttaggtgtt aaagaacaat taattttccc agaaatcgac
     6661 tatgataaag taagtaaagt tagaggaatg gatattgtta tcgtaacgac tgctaacact
     6721 gatgaagaag ctcgtgaatt gttagctaac ttcggtatgc cattccgtaa ataattattt
     6781 aaaggaggct aattaagtgg ctaaaacttc aatggttgct aagcaacaaa aaaaacaaaa
     6841 atatgcagtt cgtgaataca ctcgttgtga acgttgtggc cgtccacatt ctgtatatcg
     6901 taaatttaaa ttatgccgta tttgtttccg tgaattagct tacaaaggcc aaatccctgg
     6961 cgttcgtaaa gctagctggt aataaaaaag agtctgaaag gaggcaacaa tcaatgacaa
     7021 tgacagatcc aatcgcagat atgcttactc gtgtaagaaa cgcaaacatg gtgcgtcacg
     7081 agaagttaga attacctgca tcaaatatta aaaaagaaat tgctgaaatc ttaaagagtg
     7141 aaggtttcat taaaaatgtt gaatacgtag aagatgataa acaaggtgta cttcgtttat
     7201 tcttaaaata tggtcaaaac gatgagcgtg ttatcacagg attaaaacgt atttcaaaac
     7261 caggtttacg tgtttatgca aaagctagcg aaatgcctaa agtattaaat ggtttaggta
     7321 ttgcattagt atcaacttct gaaggtgtaa tcactgacaa agaagcaaga aaacgtaatg
     7381 ttggtggaga aattatcgca tacgtttggt aataaaaaat aaggaggtgc cataacatga
     7441 gtcgtgttgg taagaaaatt attgacatcc ctagtgacgt aacagtaact tttgatggaa
     7501 atcatgtaac tgttaaaggt cctaaaggtg aattatcaag aactttaaat gaaagaatga
     7561 cattcaaaca agaagaaaac acaattgaag ttgtaagacc atctgattct aaagaagata
     7621 gaacaaacca tggtacaact cgtgctttat taaacaatat ggtacaaggt gtttctcaag
     7681 gatacgtaaa agtacttgaa cttgttggtg taggttaccg tgctcaaatg caaggtaaag
     7741 acttaatcct taacgttggt tattctcacc cagtagaaat taaagctgaa gaaaacatta
     7801 ctttctcagt tgagaaaaac acagtcgtta aagttgaagg tatttcaaaa gaacaagttg
     7861 gagcattagc atctaacatc cgttcagtaa gacctccaga gccttacaaa ggtaaaggta
     7921 ttcgttacca aggtgaatac gttcgccgta aagaaggtaa aactggtaaa taatagataa
     7981 actctaaaga aaggagtatt gaaatgatca gtaaaattga taaaaataaa gtgcgtttaa
     8041 aaagacatgc tcgtgttcgt actaacttat caggtacagc tgaaaagcca cgtttaaacg
     8101 tatatcgttc aaacaagcat atctacgctc aaattattga tgataataaa ggcgtaacat
     8161 tagctcaagc ttcttcaaaa gacagcgaca ttgctactac agcaactaaa gttgaattag
     8221 caactaaagt cggtgaagca attgctaaaa aagctgctga caaaggcatt aaagaaatcg
     8281 tatttgaccg tggaggatat ttatatcacg gacgtgttaa agcattagct gaagcagcaa
     8341 gagaaagcgg attagaattt taatttaaag gagggacaaa tacatggctc gtagagaaga
     8401 agagacgaaa gaatttgaag aacgcgttgt tacaatcaac cgtgtagcaa aagttgtaaa
     8461 aggtggtcgt cgtttccgtt tcactgcatt agttgtagtt ggagacaaaa atggtcgtgt
     8521 aggtttcggt actggtaaag ctcaagaggt accagaagca atcaaaaaag ctgttgaagc
     8581 agctaaaaaa gatttagtag ttgttccacg tgttgaaggt acaactccac acacaattac
     8641 tggccgttac ggttcaggaa gcgtatttat gaaaccggct gcacctggta caggagttat
     8701 cgctggtggt cctgttcgtg ccgtacttga attagcaggt atcactgata tcttaagtaa
     8761 atcattagga tcaaacacac caatcaacat ggttcgtgct acaatcgatg gtttacaaaa
     8821 ccttaaaaat gctgaagatg ttgcgaaatt acgtggcaaa acagtagaag aattatacaa
     8881 ttaaggaggg aaaactagtt atggctaaat tacaaattac cctcactcgt agtgttattg
     8941 gtcgtcctga aacacaacgt aaaactgttg aagctttagg tcttaaaaag actaacagtt
     9001 cagtagttgt tgaagataac cctgctattc gtgggcaaat caacaaagtt aagcacttag
     9061 taacagtaga agaaaaataa tcaaggaggt gccgaaatga aattacatga gttaaaaccg
     9121 gcagaaggtt cacgtaaaga acgcaatcgt gttggacgtg gtgttgcgac aggtaacggt
     9181 aaaacaagtg gtcgcggaca caaaggtcaa aaagctcgtt caggcggtgg tgtaagacca
     9241 ggatttgaag gtggtcaatt accattattc cgtcgtttac caaaacgtgg ttttactaac
     9301 ataaatcgta aagaatatgc tattgttaac ttagaccaac ttaataaatt tgaagatggt
     9361 actgaagtaa ctccagcttt attagtagaa tctggtgttg ttaagaatga aaaatctggt
     9421 atcaaaatac taggtaatgg ttcacttgat aagaaattga cagtgaaagc tcataaattc
     9481 tcagcttcag cagcagaagc tattgatgct aaaggtggag cacacgaggt gatctaatga
     9541 ttcaaaccct tgtgaacttc tttagaacaa aagaggttcg taacaagatt ttcttcacac
     9601 tagcaatgtt agtaattttt aaaataggga cttatatacc agctccagga gtaaatcctg
     9661 cagcttttga taatccccaa ggttctcaag gtgccactga gttattaaat acttttggtg
     9721 gcggagcctt gaaacgattt tctatttttg caatgggtat tgtaccctac atcactgcat
     9781 caatcgtaat gcaattatta caaatggata ttgtccctaa attctcagaa tgggcaaaac
     9841 aaggtgaagt aggtagaaga aagttaaata acgttactcg ttatttagca atttctttag
     9901 catttatcca atctataggt atggcattcc aatttaataa ttatctcaaa ggtgcgctga
     9961 ttatcaatca gtcaattatg agttatttat taatagcact agttttgaca gcaggaactg
    10021 ctttcttaat atggcttggt gatcaaatca ctcagttcgg tgttggtaat ggtatttcta
    10081 ttatcatatt tgcgggtatt ttatcaacgt tgccagccag tctaattcaa tttggacaaa
    10141 ctgcgttcgt tgggcaagaa gatacatcat tagcatggtt gaaagtatta ggacttttag
    10201 tgtcactgat actattaact gtaggtgcta tttatgtact tgaagccgtt cgtaaaattc
    10261 cgattcaata tgctaagaaa caaactgctc aaagattagg ttcacaggca acttatctac
    10321 ctttaaaagt gaactcagcc ggtgttattc ctgtaatctt tgcaatggcg ttcttcttac
    10381 tgcctagaac attaacgttg ttctatccag ataaagaatg ggcacaaaac attgcgaatg
    10441 ctgcaaatcc ttcaagtaat gttggtatgg ttgtatatat tgtactaatt atactattca
    10501 catacttcta tgcatttgta caagttaatc cggaaaaaat ggctgataac cttaagaaac
    10561 aaggtagcta tgttccaggt attagacctg gtgaacaaac caaaaaatat attactaaag
    10621 ttttatatcg tttaactttt gtaggttcta tcttcttagc cgttatttca attctaccga
    10681 tattagcaac taagtttatg ggattaccac aatcaattca gattggtggt acaagtttac
    10741 ttatcgttat tggtgtagcg attgaaacaa tgaaatcttt agaagctcaa gtatctcaaa
    10801 aagaatataa aggctttggt ggtagataat tttaggaggg cacttatgaa tatcattttg
    10861 atgggtttac ctggcgcagg taaaggaact caagcaagtg aaattgtcaa gaaattccca
    10921 ataccccaca tttcaactgg tgacatgttc agaaaagcta taaaagaaga aactgaatta
    10981 ggtaaagaag ctaagtctta tatggaccgt ggcgaattag ttcctgatga agtgactgta
    11041 ggtatcgtta aggaaagaat ttctgaagac gatgcaaaaa aaggcttttt attagatggc
    11101 ttcccaagaa caatcgagca agctgaggca ttaaataata ttatgtctga gcttgacaga
    11161 aacattgatg ctgtcatcaa tatcgaagtt ccggaagaag aattaatgaa ccgtcttaca
    11221 ggtcgtcgaa tctgtgagtc atgtggtaca acgtatcatc ttgtatttaa tcctccgaag
    11281 gtcgaaggta tttgtgatat cgatggtggt aaattgtatc aacgagaaga tgataatcct
    11341 gaaacggtag ctaatcgttt gagtgttaat attaaacaat ctaagcctat tttagatttc
    11401 tatgatcaaa aaggtgtatt gaaaaatatt gatggttcaa aagatattag cgacgttacc
    11461 aaagatgtca ttgatatttt agatcatttg taatagatca acttttagcg gtctatttta
    11521 agtgaggcat gatttaatca tattagatag atgagagtgg caattaacga taattaattc
    11581 ccgtaatttt tgtttaactt ataaaattaa tcactgatag attcgtaaca ataaagttgt
    11641 ctattgacga ttaccttact attgcaaaaa gggggaagtt aatcaatggc taaacaagat
    11701 gtaattgaat tagaaggtac tgtattagat actttaccga acgcaatgtt taaagtagaa
    11761 ttagaaaatg gtcatgagat tttagctcac gtaagtggta aaatcagaat gaattacatt
    11821 cgtattctac ctggcgacaa agtaactgtt gagatgtctc cgtacgattt aacacgcgga
    11881 agaattactt atcgttataa ataatcgtca ctccataata tagggaggta taaaaatgaa
    11941 agtaagacca tcagtaaaac ctatttgcga aaaatgtaaa gtcattaaac gtaaaggtaa
    12001 agtaatggta atttgtgaaa atccaaaaca caaacaaaga caaggttaat aaaagagagg
    12061 tgtaaattaa tatggcacgt attgcaggag tagatattcc acgtgaaaaa cgcgtagtta
    12121 tctcattaac ttatatatac ggtatcggta cgtcaactgc tcaaaaaatt cttgaagaag
    12181 ctaacgtatc agctgatact cgtgtgaaag atttaactga tgacgaatta ggtcgcatcc
    12241 gtgaagttgt agacggttat aaagtcgaag gtgacttacg tcgtgaaact aacttaaata
    12301 tcaaacgttt aatggaaatt tcatcatacc gtggtatccg tcaccgtcgt ggtttaccag
    12361 ttcgtggtca aaaaacgaaa aacaacgcgc gtactcgtaa aggaccagtt aaaacggtag
    12421 ctaacaagaa aaaataatag gtaaaggagg caaattttaa atggcacgta aacaagtatc
    12481 tcgtaaacgt agagtgaaaa agaatattga aaatggtgta gcacacatcc gttcaacatt
    12541 caacaacact attgtaacta tcactgatga gttcggtaat gctttatcat ggtcatcagc
    12601 tggtgcatta ggattcaaag gatctaaaaa atcaacacca tttgcagcac aaatggcttc
    12661 tgaaactgca tctaaatcag ctatggagca tggtttaaaa acagttgaag taacagttaa
    12721 aggacctggt ccaggtcgtg aatcagctat tcgtgcatta caatctgcag gtttagaagt
    12781 aactgcgatc agagacgtta ctccagtacc tcataacggt tgtcgtccac caaaacgtcg
    12841 tcgtgtataa tttatgatgg tattgttaca ggtcactgag caaacatttt aaattaagtc
    12901 gacgtatata aggaggatat ttaaatgata gaaatcgaaa aacctagaat tgagacaatt
    12961 gaaattagtg aagatgctaa attcggtaag ttcgttgttg aaccactaga acgtggctac
    13021 ggtactacac taggaaactc cttacgtcgt atcctactat cttcattacc aggtgcagcc
    13081 gttaagtata ttgaaattga gggagtttta catgaattct cagcagtaga caatgtagtt
    13141 gaagatgttt ctacaattat tatgaacatt aaacaattag cattgaaaat ttactctgaa
    13201 gaagataaaa ctttagaaat tgatgtacgt gatgaaggcg aagtaacagc aagcgacatt
    13261 acacatgata gtgatgttga aattttaaac ccagagctta aaattgcaac agtatctaaa
    13321 ggtggtcact taaaaattcg tctagttgct aacaagggta gaggttacgc attagcagaa
    13381 caaaataata ctagtgattt accaattggt gtaatccctg ttgattcatt gtattcacct
    13441 gttgaacgtg tgaactatac tgttgaaaat acacgtgtag gtcaaagcag tgattttgat
    13501 aaattaacat tggatgtttg gactaatggt tcaatcacac cacaagaatc agtttcatta
    13561 gcagcaaaaa taatgactga acacttgaat atcttcgttg gtcttactga tgaagcgcaa
    13621 aacgctgaaa tcatgattga aaaagaagaa gatcaaaaag aaaaagtatt agaaatgtct
    13681 attgaagaat tagacttatc tgtacgttca tataactgct taaaacgcgc aggaatcaat
    13741 tctgttcaag agttagctga caaatctgaa gctgacatga tgaaagtgcg taatttaggt
    13801 cgtaaatctt tagaagaagt taaatacaaa ttagaagatt taggattagg attaagaaaa
    13861 gaagattgat aaaggaggtt aactcatggg ttacagaaaa ttaggtcgta cttctgatca
    13921 acgtaaagct atgttacgtg acttagctac atcacttatt attagtgaac gtattgaaac
    13981 tacagaagct cgtgcaaaag aagttcgcag tgttgttgag aaattaatca ctttaggtaa
    14041 aaaaggagat ttagcttctc gtcgtaatgc agctaaaact ttacgtaatg ttgaaatctt
    14101 aaacgaagat gaaactacac aaactgcact tcaaaaatta tttggtgaaa tcgcagagcg
    14161 ttacacagaa cgtcaaggtg gttacactcg tattcttaaa caaggccctc gtcgcggtga
    14221 cggtgctgaa tcagtaatta tcgaattagt ataagaattt atataaatac actaactact
    14281 tttatataaa gcaaatgagt gcaacgataa tgtttgccac atacaaatat tgtctagctc
    14341 agagtacccc atcaactaaa taaagattta aagcgtgaac tcaacaaact tatgatgggg
    14401 tgcgcgcttt tttattgaat aaaatatcgg acttatgaac agaaatgttt ttaagtccga
    14461 tttctttata tttacttttt tgagtgcgaa tctaaaaata tgatgaagct agtttgatca
    14521 atttcactag aactgaatga agtgaaagta ttatcgttga atcaaacgcg accaccccta
    14581 tattagtaaa tcatttattt tattaacgtt tatacataat tgttagagag gaataaaagt
    14641 ccttatcatt agttgttaaa caacacttta aagactagaa atgagtcaga aaatactagt
    14701 aatatataga gaacccagtc aatgtcatat acagcgattt ataaatattg taaaatatga
    14761 tagatacacg aatcgtaaag gagagagaca agtggaggat aagaattcag ttattgtatt
    14821 taaaaatgtt tcatttcaat atcaaagtga tgcatccttc acattgaaag atgtttcttt
    14881 taatatacct aaaggtcagt ggacatctat tgttggtcat aacggttctg gaaaatctac
    14941 aattgccaag ttaatgattg gcatagagaa agttaaatct ggagaaattt tttataataa
    15001 tcaagctata actgatgata attttgaaaa gttaagaaaa gacataggaa ttgtatttca
    15061 gaatccggat aatcaatttg ttggttcaat tgtaaaatac gatgtggcat ttggactcga
    15121 aaatcatgcg gttccatatg acgaaatgca tagaagagtc agcgaagcac ttaaacaagt
    15181 tgatatgtta gaacgtgcag attatgaacc taatgcatta tcggggggac agaagcagcg
    15241 tgtggctata gcaagtgtat tagcacttaa cccctctgtc attatattag atgaggcgac
    15301 ttctatgtta gatcctgatg cacgtcaaaa tttattggat ttagtgagaa aagttaaatc
    15361 agaacataat attacaatca tttctattac gcatgattta tctgaggcga tggaagcaga
    15421 tcatgttatc gttatgaata aagggactgt ctataaagaa ggcacagcga ctgaaatttt
    15481 cgacaatgca gaagagttaa caagaatagg tctagatttg ccattcccaa tcaaaataaa
    15541 tcaaatgctg ggacaccaaa catcattctt aacttatgaa gggctggtgg atcaactatg
    15601 actatacggt ttgacaatgt aagttatacc tatcaaaaag ggacaccata tcagcatcaa
    15661 gctattcatg atgttaatac agaatttgaa caaggtaaat attacgccat cgttggacaa
    15721 acgggtagtg gtaaatcaac gttgatacaa aatattaatg cgctgttaaa gccgactact
    15781 gggacagtta cagttgatga cattactatt acacataaga ccaaagataa atatattaga
    15841 cctgtaagaa aaagaattgg aatggtattt caatttcccg aatctcaatt atttgaggac
    15901 acagtagagc gtgaaatgat atttggacct aaaaacttta aaatgaattt agatgaagcc
    15961 aaaaactatg cccatcgtct gttgatggat ttaggctttt caagagatgt aatgtcacaa
    16021 tcaccatttc aaatgtcagg tggacaaatg cgtaaaatag cgattgtatc gatattggca
    16081 atgaatcctg atattatcgt ggttgatgaa cctacagcag gacttgatcc acaaagtaaa
    16141 cgacaagtaa tgagattact aaagtcacta caaacagatg aaaataaggc aattatccta
    16201 atttcacatg atatgaatga agtcgcgcgt tatgcagatg aagtcatcgt tatgaaagaa
    16261 ggtagtatag tgtcgcaaac atcaccaaaa gagctcttca aagataaaaa aaaattggcg
    16321 gattggcata ttggtttgcc agaaattgtt cagttacaat atgactttga acaaaaatat
    16381 caaacaaaat taaaagatat tgccttaact gaagaagcat ttgtaagctt gtataaggag
    16441 tggcaacatg aaaaataaat tgattatagg gcgatattta ccaattaatt cttttgttca
    16501 tcatcttgat ccaagagcaa agttaatgtt tgtttttctt tttattatat taatattttt
    16561 ctgtcattca ccattaacat atctttgggt gtttgcactt atcttattca ttatgagatt
    16621 agcaaaaatt caattatggt tcctaatcaa aggtttaaca ccaatatttt tctttttaat
    16681 tttcacatta atgatgcata tatttttaac taaaggtgga tatgtattag ttgaatggca
    16741 tggcatcacg attgaaacta atggtatttt agaagggcta tatatatcac tgcgtttaat
    16801 tgggattgta atgattgcaa caattatgac actatctaca agtccaattg atttaacaga
    16861 tgcgtttgaa agattacttg caccactaaa gatgtttaaa ttaccagttc atcaattaag
    16921 tatgataatg tccattgcgt tacgattcat cccgacgtta atggatgagt tagataaaat
    16981 cattttggcg caaaagtcgc gtggttctga aataagttca gggaacattg caacacgtat
    17041 taaatcattt attccattac tagtgccact attcatctca gctttccaac gcgccgaaga
    17101 attagcggtc gcaatggaag ttaggggtta tgatgccaat gttaagagaa cgagctacag
    17161 acagcttaaa tggcaattga gagatacgat atctttaacc atgattatcc caattgcaat
    17221 tattttattc gtattaaaat attcaggagt gtaaattcat gcgtatatta gtagaaattg
    17281 cgtatcaagg aaataatttt ctaggttttc aaattcaaca aaatggtcga acggtacagc
    17341 aacaatttga aaagctatta caacgcatgc ataaaagaca tgtgagaatc catccttcaa
    17401 gtaggactga tagaggtgta catgcgatac agcaatactt tcatttcgac acagagctga
    17461 atatacctat gtcacaatgg caatatgcaa tgaaccgaac gttacctgat gatatttatg
    17521 tcaacaatgt agttacagtc gatgacgatt ttcattgtcg ttatgattgt gtaggtaaac
    17581 gttatcgcta caaagtatat caagcacaac accgcgatcc atttcaaagt ggtttgaaaa
    17641 catttattcc tgaaacgttg gatttaggta aaatgaacag agcggcgcaa caattcatag
    17701 gtacacatga ttttacggga ttttgttcgc aaaaaactga agtagaaagc aaagttagaa
    17761 cattatacca aagtgaaatc gtaaagacag atgatggctt tgattatatt gtcactggat
    17821 ctggattttt atataacatg gtgcgtgtct tagttgcatt tttaatagaa gttggaaaag
    17881 ggcgacatga agtatctgat gtaccaaaac ttcttgaatc aaagaatcgt aagaatgtac
    17941 ctttcactgc accggctgaa ggattatatt tagagaaaat ttatttagat gaaaatgagt
    18001 tattaaaaga ctttggtaac gatatcaaaa tacatcgtaa aaaatcatta caaaatgact
    18061 gattgccatt gacaaaaagc ttgtgaaatt ataagattat taacggtatt gttttatatc
    18121 caccccacga taagccccgg aaacttattg tgttacaaga tatataagca gaaacgaaca
    18181 acagttaaca aaataaatga aattaaacgt tttaaaaatg aaacaaatga aatcatctat
    18241 taggttatga aactgtttat agcttgaata gaagcattta ttttttagga ggacaattat
    18301 tatgcgtcaa acatttatgg caaatgaatc aaacattgag cgcaaatggt atgttatcga
    18361 tgctgaaggc caaacattag gtcgtttatc atcagaagta gcatctatct tacgcggtaa
    18421 aaataaagta acttacacac cacacgttga tactggtgat tatgtaatcg ttattaatgc
    18481 atcaaaaatc gaatttactg gtaacaaaga aactgacaaa gtttactacc gtcactcaaa
    18541 tcacccaggt ggtatcaaat caatcactgc tggtgaatta agaagaacta acccagaacg
    18601 tttaattgaa aactcaatta aaggtatgtt accaagcact cgtttaggcg aaaaacaagg
    18661 taaaaaatta tttgtatatg gtggcgctga acatccacac gctgcacaac aaccagaaaa
    18721 ctacgaatta cgtggttaat tagaaggagg aaatgacttt ggcacaagtt gaatatagag
    18781 gcacaggccg tcgtaaaaac tcagtagcac gtgtacgttt agtaccaggt gaaggtaaca
    18841 tcacagttaa taaccgtgac gtacgcgaat acttaccatt cgaatcatta attttagact
    18901 taaaccaacc atttgatgta actgaaacta aaggtaacta tgatgtttta gttaacgttc
    18961 atggtggtgg tttcactgga caagctcaag ctatccgtca cggaatcgct cgtgcattat
    19021 tagaagcaga tcctgaatac agaggttctt taaaacgcgc tggattactt actcgtgacc
    19081 cacgtatgaa agaacgtaaa aaaccaggtc ttaaagcagc tcgtcgttca cctcaattct
    19141 caaaacgtta attgtcggac gatatataca aaacacctcg atattatgtc gaggtgtttt
    19201 tttgcgtttt tgcggcgaat atggaatgtg tagaatataa atgaattttt accttcccac
    19261 cataaaagat gaagaaccat gaaatgtgga gaacaataaa tagttgatat tctgttattt
    19321 ttttggaagt gaagtgattt gaatacttta ctctaaacga taaaaggtta aaaaaacaac
    19381 aaaaagaaaa tattactgtc ataattggaa atacaccgta ttctgctggt caatctagtg
    19441 taaataatga taataaaaaa atgaatatga aaaattaaat gcaaaaatag caaataccta
    19501 tgcaaatttt tcaacagcaa atttaaaaaa atcattatat gattcgtata tacaggcttt
    19561 tcgatgggca actgatagag ttggaaaaaa ggtattatag gatttgtaac aaatggatca
    19621 tttattgata gtcaaagcac tgatggattt agaaaagttt tatacgatga atttaactat
    19681 ctgtacataa ttaatttgag aggcgaccaa cgaacacagg gtgaaaaatc aagaaaagaa
    19741 ggtggaaaaa tatttggatc aggaagtaga gcaccgatag ctatatctat tttagttaaa
    19801 gatggaagtt acaatcacga tatctattat aatgacattg gtgaatattt aactagagag
    19861 caaaaattag atactttaat gaaacatcaa tcaattgtga atttgaaaag tttgaatgtt
    19921 ttacccgata aaaacaatga ttggattaat caacgagaca taaactatga gaattattta
    19981 ccaatgtatg attccaagga tattgaaaat tctatatatt tagatcaatt taatggagta
    20041 aattcagcaa gagataattg ggtaacaaat ttttcgaatg aaaaagcatt agtaaatgca
    20101 aaattacttg tagataacta taattctgaa atagatagat taattgatat cttagattca
    20161 agagaacgta taaatttagt gaacaaagat gaaacattta ttagttggac acgaggactt
    20221 acccaaaaat tttctaaagg aaaaaatatt tcaattaatc cagagagaat tgttaaattt
    20281 atgcatagac catttacaaa aaaatggata gtgtacgaca aaaatattat ggaaatgcca
    20341 agtagatatt ataatataat ggagaatact ggacaggtga tttatataca aggacaggga
    20401 atgaataaag aattctcggc tatgatcacg gacatattac ctaatttcca atttattggt
    20461 aatggcaaag gctttgctac atacaaaggt aaagatagtt tgaggctt
//
LOCUS       DAXWFK010000029        20488 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.029, whole genome shotgun sequence.
ACCESSION   DAXWFK010000029 DAXWFK010000000
VERSION     DAXWFK010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20488
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.029"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            211..657
                     /locus_tag="ACGEXS_000857"
     CDS             211..657
                     /locus_tag="ACGEXS_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFF8011891.1"
                     /translation="MYVENSYLSKQLCFLFYVSSKEIIKKYTNYLKEYDLTYTGYIVL
                     MAIENDEKLNIKKLGERVFLDSGTLTPLLKKLEKKDYVVRTREEKDERNLQISLTEQG
                     KAIKSPLAEISVKVFNEFNISEREASDIINNLRNFVSKNFDYSDKK"
     gene            873..1301
                     /locus_tag="ACGEXS_000858"
     CDS             873..1301
                     /locus_tag="ACGEXS_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFF8011892.1"
                     /translation="MILNFNQFENQNFFNGNPSDTFKDLGKQVFNYFSTPSFVTNIYE
                     TDELYYLEAELAGVNKEDISIDFNNNTLTIQATRSAKYKSEQLILDERNFESLMRQFD
                     FEAVDKQHITASFENGLLTITLPKIKPSNETTSSTSIPIS"
     gene            complement(1408..2577)
                     /locus_tag="ACGEXS_000859"
     CDS             complement(1408..2577)
                     /locus_tag="ACGEXS_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFF8011893.1"
                     /translation="MYKTKGGFQLTLQTLSLVVGFMAWSIIAPLMPFIKQDVNVTEGQ
                     ISIILAIPVILGSVLRVPFGYLTNIVGAKWVFFTSFIVLLFPIFFLSQAQTPGMLMAS
                     GFFLGVGGAIFSVGVTSVPKYFPKEKVGLANGIYGMGNIGTAVSSFLAPPIAGIIGWQ
                     TTVRSYLIIIALFALIMFIFGDTQERKIKVPLMAQMKTLSKNYKLYYLSYWYFITFGA
                     FVAFGIFLPNYLVNHFGIDKVDAGIRSGVFIALATFLRPIGGILGDKFNAVKVLMIDF
                     VVMIIGAIILGISDHIALFTVGCLTISICAGIGNGLIFKLVPSYFLNEAGSANGIVSM
                     MGGLGGFFPPLVITYVANLTGSSHLAFIFLAVFGCIALFTMRHLYQKEYGSLKNG"
     gene            2947..3861
                     /locus_tag="ACGEXS_000860"
                     /pseudo
     CDS             2947..3861
                     /locus_tag="ACGEXS_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276756.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
     gene            3840..4580
                     /locus_tag="ACGEXS_000861"
     CDS             3840..4580
                     /locus_tag="ACGEXS_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFF8011894.1"
                     /translation="MAYTYTLKDIIEITGVTKRTLHYYDEIGLLVPDKNDKNYRVYKQ
                     QDLEKLQKILILKSFDFDIAKIKQYISYDNEQLRKLLSEQISKLDKKISDLQLIRRSV
                     CEFIKGLSLIDTSILNKTLQSQYDKEASIKYGHTKAYQSFIRRKDSLQSQDIRHKLTT
                     IFNKFNHMSLSHYPIQDCSDLVFEWKAFMNTIADFDDETLCCIAKTYEDDTRFKDYFN
                     SYDNQNLASYISEAVNYFLSNVNKSDNF"
     gene            complement(4838..5491)
                     /gene="nreC"
                     /locus_tag="ACGEXS_000862"
     CDS             complement(4838..5491)
                     /gene="nreC"
                     /locus_tag="ACGEXS_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002464689.1"
                     /note="Involved in the regulation of the the nitrate
                     reductase operon narGHJI; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation response
                     regulator NreC"
                     /protein_id="HFF8011895.1"
                     /translation="MKIVIADDHAVVRTGFSMILNYQNDMEVVATAADGVEAYQKVME
                     YKPDVLLMDLSMPPGESGLIATSKIADSFPETKILILTMFDDEEYLFHVLRNGAKGYI
                     LKNAPDEQLLLAIRTVYKGETYVDMKLTTSLVNEFVSNSNQDTANTTDPFKILSKREL
                     EILPLIAKGYGNKEIAEKLFVSVKTVEAHKTHIMTKLGLKSKPELVEYALKKKLLEF"
     gene            complement(5514..6548)
                     /gene="nreB"
                     /locus_tag="ACGEXS_000863"
     CDS             complement(5514..6548)
                     /gene="nreB"
                     /locus_tag="ACGEXS_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501138.1"
                     /note="Involved in the regulation of the the nitrate
                     reductase operon narGHJI; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation sensor histidine
                     kinase NreB"
                     /protein_id="HFF8011896.1"
                     /translation="MINEDSIQLDTLLKKYYEHSIEKIVFADDNGKIIAMNDAAKDIL
                     SEEDNYSAVANAICHRCEGYTNAYDVQSCKDCFLESMQVQATNFQVFMKTKDQKVMPF
                     TATYQLIDQDRGIHAFTLQNVSSQIEQQEKLHQQRMMRKTISAQENERKRISRELHDS
                     VIQEMLNVDVQLRLLKYQEDTTKLLEDAENIEYIVAKLIDDIRNMSVELRPASLDDLG
                     LEAAFKSYFKQFEENYGIKIIYTSNIKNTRFDSDIETVVYRVVQEAILNALKYADVNE
                     INVGIRQTGRHLVAEVIDAGNGFDPSSKPKGSGLGLYGMNERAELVSGSVNIETKIGE
                     GTNVTLNIPI"
     gene            complement(6572..7024)
                     /gene="nreA"
                     /locus_tag="ACGEXS_000864"
     CDS             complement(6572..7024)
                     /gene="nreA"
                     /locus_tag="ACGEXS_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation accessory nitrate
                     sensor NreA"
                     /protein_id="HFF8011897.1"
                     /translation="MTPEAIIEDRRFQETLDKIRKEEGYDFAAIAFYESNKPSSPIKW
                     HYVSGNKNNRFKLIILRKGRGLAGTVMKTGKRMVIANVGLALGPEEKIDYPILLSESL
                     TAVLAVPLWYKNQVYGVLLFGQRDGRPLPKIFDNDDIQRKFGIFNDDK"
     gene            complement(7044..7721)
                     /gene="narI"
                     /locus_tag="ACGEXS_000865"
     CDS             complement(7044..7721)
                     /gene="narI"
                     /locus_tag="ACGEXS_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFF8011898.1"
                     /translation="MLNQFLWVIFPYLCLVVFVAGHIARYRYDKFSWTAKSSELIERK
                     RLMWGSLLFHLGIIPVFFGHVVGLLIPKPWMDAVGVSEHLYHIGAVYIGSIFGIITLI
                     GMFLLTARRVTTKSIRRLSSASDIFVNFLLLLIVFMGCYATLVTNIQHPDFNYRTSLS
                     IWFRQLFMLKPDASLMSGVPIAFKMHILLGFTIMACWPFTRLVHVWSVPLTYINRRYI
                     IYRKNKV"
     gene            complement(7714..8289)
                     /gene="narJ"
                     /locus_tag="ACGEXS_000866"
     CDS             complement(7714..8289)
                     /gene="narJ"
                     /locus_tag="ACGEXS_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFF8011899.1"
                     /translation="MINFDNFKKYQESFGYMAQQLCFPEKLTFHPKTFEETISKDHPA
                     YEDLVAFRNVMMTFSLSEIKAIYTDTFDFTKKAPLYMTYNKFDTQKERGQMLAKLKVL
                     YEMFGLEMVDNELSDYLPLMLQFLQVAEWRNDPRAEENIQLIIMIIEDGTYVMANALG
                     EDNNPYAYVIQALRKTLKLCLTSPKGVKHHA"
     gene            complement(8282..9841)
                     /gene="narH"
                     /locus_tag="ACGEXS_000867"
     CDS             complement(8282..9841)
                     /gene="narH"
                     /locus_tag="ACGEXS_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFF8011900.1"
                     /translation="MKIKAQVAMVLNLDKCIGCHTCSVTCKNTWTNRPGAEYMWFNNV
                     ETKPGVGYPKRWEDQEHYKGGWVLNRKGKLELKSGSRISKIALGKIFYNPDMPLIKDY
                     YEPWNYNYEHLTTAKSGKHSPVARAYSEITGDNIEIEWGPNWEDDLAGGHVTGPKDPN
                     IQKIEEDIKFQFDETFMMYLPRLCEHCLNPSCVASCPSGAMYKRDEDGIVLVDQDACR
                     GWRYCMTGCPYKKVYFNWKTNKAEKCTFCFPRIEAGMPTVCSETCTGRMRYLGVLLYD
                     ADRVHEAASAVDEKDLYEKQLDIFLNPFDEEVIAQAEKDGIGYDWIEAAQNSPIYKLA
                     IEYKLAFPLHPEFRTMPMVWYCPPLSPIMSYFEGKNTTQNPDAIFPAIEEMRLPIEYL
                     ANIFTAGDTEPVKGALQRMAMMRSYMRSQVTQQPFDTSRLERLGITERQTKDMYRLLG
                     LAKYEDRFVIPTSHKETYLDTYHAQGSTGYNYGGEHFGDNCEGCGVAVGSGKTGQEIY
                     NENFYGGIFRD"
     gene            complement(9831..13520)
                     /locus_tag="ACGEXS_000868"
     CDS             complement(9831..13520)
                     /locus_tag="ACGEXS_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFF8011901.1"
                     /translation="MGKFGLNFFKPTEKFNGNWSILESKSREWEKMYRERWSHDKEVR
                     TTHGVNCTGSCSWKVFVKNGVITWENQQTDYPSCGPDMPEYEPRGCPRGASFSWYEYS
                     PLRIKYPYIRGKLWDLWTEALEENNGNRVAAWASIVENEDKAKQYKQARGMGGHVRSN
                     WKDVTEIIAAQLLYTIKKYGPDRIAGFTPIPAMSMISYAAGARFINLLGGEMLSFYDW
                     YADLPPASPQIWGEQTDVPESSDWYNASYIIMWGSNVPLTRTPDAHFMTEVRYKGTKV
                     ISVAPDYAENVKFADNWLAPNPGSDAAIAQAMTHVILQEHYVNQPNERFINYAKQYTD
                     MPFLIMLDEDENGYKAGRFLRASDLGQTTEQGEWKPVIHDAISDSLVVPNGTMGQRWE
                     EGKKWNLKLETEDGSKINPTLSMTEGGYELETIQFPYFDSDGDGIFNRPIPTRQVTLA
                     NGDKVRIATIFDLMASQYGVRRFDHKLESKGYDDAESKYTPAWQEAISGVKQSVVIQV
                     AKEFAQNAIDTEGRSMIIMGAGINHWFNSDTIYRSILNLVMLCGCQGVNGGGWAHYVG
                     QEKCRPIEGWSTVAFAKDWQGPPRLQNGTSWFYFATDQWKYEESNVDRLKSPLAKTEE
                     LKHQHPADYNVLAARLGWLPSYPQFNKNSLLFAEEAKDEGIDSNEAILQRAIDEVKSK
                     QTQFAIEDPDLKKNHPKSLFIWRSNLISSSAKGQEYFMKHLLGTKSGLLATPNEDEKP
                     EEITWREETTGKLDLVVSLDFRMTATPLYSDIVLPAATWYEKHDLSSTDMHPYVHPFN
                     PAIDPLWESRSDWDIYKTLAKAFSEMAKDYLPGTFKDVVTTPLSHDTKQEISTPYGVV
                     KDWSKGEIEAVPGRTMPNFAIVERDYTKIYDKYVTLGPVLEKGKVGAHGVSFGVSEQY
                     EELKSMLGTWSDTNDDSVRANRPRIDTARNVADAILSISSATNGKLSQKSYEDLEEQT
                     GMPLKDISSERAAEKISFLNITSQPREVIPTAVFPGSNKQGRRYSPFTTNIERLVPFR
                     TLTGRQSYYVDHEVFQQFGESLPVYKPTLPPMVFGNRDKKIKGGTDALVLRYLTPHGK
                     WNIHSMYQDNKHMLTLFRGGPTVWISNEDAEKHDIQDNDWLEVYNRNGVVTARAVISH
                     RMPKGTMFMYHAQDKHIQTPGSEITDTRGGSHNAPTRIHLKPTQLVGGYAQISYHFNY
                     YGPIGNQRDLYVAVRKMKEVNWLED"
     gene            complement(13829..14806)
                     /gene="cobA"
                     /locus_tag="ACGEXS_000869"
     CDS             complement(13829..14806)
                     /gene="cobA"
                     /locus_tag="ACGEXS_000869"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFF8011902.1"
                     /translation="MSVEEYGKVYLIGAGPGNPNYLTKKAERLIREADVILYDRLVNP
                     LILQYANLTTEIIDVGKKPYAKHIQQEKINDCIVEAARRYNKVVRLKGGDPAIFGRVQ
                     EEVDTLNEHHIAFEIVPGVTSASAAVATMQTGLTMRTVAKSVTFSTGHFKDSEENEVD
                     VNSLVNGGTLAIYMGVKRLGKIITQIQQYTDIDYPIAIVFQASCFNEFVVKGRLSNII
                     SKLQHYSIEAKPGICIIGEVVDYTENTPKSYDPMKQFYVVSGSKHDALMLCEHLYDEG
                     YGCLLNPNDTSNGTYHSSQYDYYDAFIKQQENVTYISTDRADANTVLCH"
     gene            complement(14797..15111)
                     /gene="nirD"
                     /locus_tag="ACGEXS_000870"
     CDS             complement(14797..15111)
                     /gene="nirD"
                     /locus_tag="ACGEXS_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFF8011903.1"
                     /translation="METKEKIKVTTIDELTPLIGKKVIVKGKEVGLFLTESGKIHAIH
                     NICPHKQGPLSEGTVSGEYVFCPLHDQKIDLNTGIVQEPDEGCVDVYEVEVTDGNVYI
                     CL"
     gene            complement(15115..17520)
                     /gene="nirB"
                     /locus_tag="ACGEXS_000871"
     CDS             complement(15115..17520)
                     /gene="nirB"
                     /locus_tag="ACGEXS_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HFF8011904.1"
                     /translation="MAKQKLVMIGNGMAGIRTIEEILERANDLYDITVIGKEPYPNYN
                     RIMLSNILQNKMTVEETIMNPYEWYEEHGIELITNDPVIEVDRANQSVTTANGIEVSY
                     DKLIFATGSKAFVIPVPGSTLPSVIGWRTIDDTEQMMNIAKTKKKAIVIGGGLLGLEC
                     ARGLLDQGMEVTVLHLAEWLMEMQLDRKAGNMLKADLEKQGMKFEMQANTTEILGEDD
                     VEGVKLADGREIPADLVVMAVGIRPYTEVAKESGLDVNRGIVVNDVMQTSDSNVYAVG
                     ECAEHNGKVYGLVAPLYEQGKVLADHLTNKETNGYKGSTTFTSLKVSGCDLYSAGQIV
                     ENAEIKGIEIFNSVDNNYKKIFLKDGNVVGAVLYGDIDDGSRFYNMMKKGESTEDYTL
                     VSLLTKGGEEASLSIADMADDETICGCNGVDKGTIVNAITENGFTTVEEVTAKTKAGN
                     SCGKCKPQIAQILQHTLGDDFVAAKPAGICGCTDLTRDQIVTQIRAKGLKTSKEVRHV
                     LNFKNKGGCPKCRPAINYYLNMVYPHDHEDERESRFANERYHANIQNDGTFSVIPQMR
                     GGVTDADQLIRLGEVAKKYHVPLVKVTGSQRVGLYGVKKEELPNIWEDLGMRSASAYG
                     KKTRSVKSCVGKEFCRFGTQYTTRLGIRLEKTFEYIDTPHKFKMGVSGCPRSCVESGV
                     KDFGIISVENGFQIYIGGNGGTEVEKAEFLTTVETEDEVIKLCGALMQYYRETGIYAE
                     RTAPWLRRLGFENVKEVLLDPERQNELFERIMDAKKAVEAEPWEAITSNAQARRIFEV
                     EKV"
     gene            complement(17587..18318)
                     /locus_tag="ACGEXS_000872"
     CDS             complement(17587..18318)
                     /locus_tag="ACGEXS_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HFF8011905.1"
                     /translation="MNGNIIVAHGMRHGRQNQALEAFISELVKDDIHHYDIAFLESEH
                     QDLETVMTTLIQSGVDHFKIVPLLIFSAMHYLKDIPNIVHEMKRRYPDIKVEVSEPLG
                     THPLMRRLIEQRICDALAGDEQQVGVMVVAHGNINGKFTKAHEELQICAQNLQISKPT
                     YARTLYGEISFTHDLESISKRYQKLIVVPLFLYDGRLVNKVKQQMNDMVINTDIHFTP
                     SINFDPILKQIINDRLESLMIPMKI"
     gene            complement(18548..19084)
                     /locus_tag="ACGEXS_000873"
     CDS             complement(18548..19084)
                     /locus_tag="ACGEXS_000873"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8011906.1"
                     /translation="MTKTMIRLASEQDAEKLQQLMHEAFTPLRELGIDWPSVNANLDA
                     VKENIDKNTTFVMTIDDGIISTITVRYPWGSVKSISGYPFVWWFATNPEYEGKGYGSQ
                     LLTYVEEAFLRDTLKSAAVTLGTSARLHPWLLKIYEKRGYEIYSEHENDDGDLGVIMR
                     KVLIPERFDETILGQPPF"
     gene            complement(19317..20141)
                     /locus_tag="ACGEXS_000874"
     CDS             complement(19317..20141)
                     /locus_tag="ACGEXS_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFF8011907.1"
                     /translation="MIENKKTVEDTYSTGAIVDSISSSVQMKQVMVQQTPGRYMLKAM
                     MAGFLLSIVTVFMFGIKTQFASTHVDGLINLMGAIAFSLGLILVVLTNSELLTSNFMY
                     FTVGWYYKVVSVKKMTWILLYCFLGNILGGFVLFFLMKFAHVMTPEMTQALTALVQKK
                     TVDSTWLNIFTKGIFCNFFINIGIFISMQFKGGLTKAFFIACGVVVFVYMGYEHVVFN
                     AGLYAGMVFFNLDAVSWLHVLKNIVFAFLGNFVGGGIFVGLVYAFLNGKRNRLEQQ"
     gene            complement(20358..>20488)
                     /locus_tag="ACGEXS_000875"
     CDS             complement(20358..>20488)
                     /locus_tag="ACGEXS_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011908.1"
                     /translation="SSISVTSKCVPNKVIRFILRTAIGYSVFAYGLHYFSNLKKNK"
BASE COUNT         6448 a         4051 c         2956 g         7033 t
ORIGIN      
        1 tttaacaatt aaggatgata caattacatt tttttcaatt taaaaaatca gtcatatcag
       61 taattaccga tgaaaatact aagatatcga tatattttgc attatttaac atttaaattc
      121 aattatagac aattcaataa ttgcgatgaa atcagtactt gacaactatt acacttcata
      181 atattgttta ttttaacaag gagtgattgg atgtatgtag aaaacagcta tcttagcaaa
      241 cagttgtgtt ttttatttta tgtttcttca aaagaaataa ttaaaaaata cacaaactat
      301 cttaaggaat atgatttaac ctatactggt tacattgttt taatggcgat tgaaaatgat
      361 gaaaaactta acatcaaaaa attaggtgaa cgtgtgttct tagattctgg aacactgaca
      421 ccattactaa agaaattaga aaagaaagat tacgttgttc gaacacgtga agagaaagat
      481 gaaagaaacc tacaaatttc tctaacagaa caaggtaaag caataaaaag ccctcttgct
      541 gaaatttctg ttaaggtctt taatgaattc aacatttcag aacgagaagc atcagatata
      601 attaataatt tgcggaattt tgtttctaaa aattttgact attccgacaa aaaataactc
      661 cctatcataa ggtgatgggc agaagtatgc atcgatatgt ttattcatta tctttattgc
      721 ttgtataaat ctcgatgctc agttcttttg ccctgattcc ttatcaggca caaatctacc
      781 catttattac ttttttatct ccccttttac tttaactttt ttaatactac tcatataatg
      841 gctttgtaat acaaatttct ataatggaag tgatgatatt gaacttcaat caattcgaga
      901 atcaaaactt ttttaacggt aatccaagtg atacatttaa agatttaggt aaacaagtat
      961 ttaattactt ttcaacacct tcatttgtaa cgaatatata tgaaacagac gaattatatt
     1021 acttagaagc tgaactagca ggtgtaaata aagaagatat tagtatcgat ttcaataata
     1081 atacgctcac tattcaagct actagaagcg caaaatacaa atctgaacaa ctcattttag
     1141 atgagcgtaa cttcgaatca ttaatgcgtc aatttgattt tgaagctgtt gataagcaac
     1201 atattactgc tagttttgaa aatgggttat taaccattac cttgcctaaa atcaaaccaa
     1261 gcaatgaaac tacttcatca acatctattc caatttcata gttaaattta aaatgaatac
     1321 actactaaag ccttaatgtc atgctacgaa tattgcgcat gatattaagg ctttgtattt
     1381 aactaaatga atggcatgta ttacatatca accgtttttc aatgagccat attctttttg
     1441 gtataaatga cgcatggtaa ataatgcaat acatccaaat accgctaaga aaataaatgc
     1501 taaatgactt gatcctgtta aattagctac gtacgtgatt actagtggtg ggaagaatcc
     1561 tcctaaacca cccatcattg atacgatacc atttgcggat cccgcttcat ttaagaagta
     1621 tgatggtact aatttgaaga ttaagccgtt accaatacct gcacaaatac ttattgttaa
     1681 acagcctaca gtgaataatg cgatatggtc tgaaatacct aaaataatgg caccgataat
     1741 cataacaaca aaatcaatca tcaatacttt aactgcatta aatttatcac ctaaaatgcc
     1801 acctattggt cttaagaatg ttgccagcgc aatgaataca cctgatcgaa taccagcatc
     1861 tactttgtca attccaaaat gatttactaa gtagttaggt aagaaaatac caaatgctac
     1921 aaaagcacca aaagtaataa aataccaata acttaagtaa tataatttgt agtttttaga
     1981 taatgttttc atttgagcca ttaatggtac tttaatttta cgttcttgtg tgtcaccaaa
     2041 aatgaacata attaatgcaa ataaagcgat aataattaag taacttctaa ctgttgtttg
     2101 ccaaccaata atacccgcta tcggtggtgc taaaaatgaa gaaactgctg taccgatatt
     2161 tcccatacca taaataccat ttgctagacc tactttttct ttagggaaat atttaggaac
     2221 tgatgtaaca ccaactgaga aaattgcacc acctacacca aggaaaaatc ctgaagccat
     2281 taacataccc ggtgtttgtg cttggcttaa gaaaaatatc gggaataaca atacgataaa
     2341 actagtaaag aatacccatt tagcgccaac aatgtttgtt aaataaccaa atggcacacg
     2401 gagcaccgat cccaaaataa ctggtatcgc taaaatgatt gatatttgac cttcagtaac
     2461 attgacatct tgtttaataa agggcattaa tggcgcaatt atactccaag ccataaaccc
     2521 aaccactaaa cttaatgttt gtaatgtaag ttgaaagcca ccttttgttt tgtacatacg
     2581 tttcaccttc atcaaaatca tttatttttt aatagatatc aatactctgc ctatatatta
     2641 gaggtcaatg ttatttaaaa atgctttcat ttatatgaaa acattcaata tagacaaagt
     2701 attgactaca tatatatttt attatgattg tgaaaaagtg aacataagga ttttccctta
     2761 tttatgaagt gatttaccct atacaatgtc cctttaagat gcttttagac acttattcgc
     2821 catcatttta atcatattat ttaggaacac tcacatactt ttcttaattt ttttcaatta
     2881 gttacaatta ctgcttgaaa atcacgttac gttatccttt atgctgacaa cataaggagg
     2941 tttatcatgt ataaaatcaa tattattaga tcagattccg tctataataa tatactcaag
     3001 gcaccaataa acgaccgtga ttcaattttc actaaagaaa tattagtacc ttttaaaaag
     3061 aaatttgagg tacaacatat gcctatttat aatgatgaca aacagacaat gagtgcgatt
     3121 caatttttag atgcctttca aatttctccc aaagatttac gcatgtctga ccaaatgtca
     3181 atacaatacc tgaataatga tttctggagt aattgcgaaa aatatttaaa agttgctata
     3241 gatcaatttt caaattatag tatctcatct caagtttcta actatcattt cacagtatta
     3301 cttggagatc gccaaaaacc acttatgtat ctaaataaaa atcgcggtgg tgatggtggc
     3361 ataccaggtt atattatgat ttatttagtg ccgagtacaa gtacaattaa ttctatgaaa
     3421 agcttaattg cacatgaagt aaatcataat atgcgctatc aatatattga ttgggatggc
     3481 ggaagtttga ttgaattgat tattgcagaa ggattagctg aaaactatgt agaatcattg
     3541 tatggtaaag cgcatattgg accgtgggta acaaatacta attgaagtcg tgataacgta
     3601 aaaattaaaa atactattta taatcattta catttaaagc atatatttga atcgatgcct
     3661 tatctctatg gtgatgatat taataaactt caaggtaggc ctatcgttgg cttatctcat
     3721 gctgccgggt atgcatgtgg ctatcacttg gtaaaatact ttttacaaaa aacaaacata
     3781 cctattgaag ttgctacaac acttccagca caaaaaataa taaatgaggt gactgaattt
     3841 tggcatacac atacacttta aaagatatta ttgaaattac aggtgtaact aaaagaactt
     3901 tacattatta cgatgaaata ggattattag ttccagataa aaatgataaa aattatcgcg
     3961 tttataaaca gcaagactta gaaaaattac aaaagatttt aatactcaag tcttttgatt
     4021 ttgatatcgc taaaataaaa caatacattt cgtatgataa tgaacaattg cgaaagttat
     4081 tatcagagca aataagcaag ttagataaaa agatttctga cttacaatta attagacgct
     4141 ctgtatgtga atttattaaa ggactctctc taatagatac cagcatttta aacaagacac
     4201 tacagtcaca atatgataaa gaagcatcta taaaatatgg tcatacgaaa gcatatcaat
     4261 catttattag acgtaaagac agcttacaat cgcaggatat cagacataaa ttgacaacta
     4321 tcttcaataa atttaatcat atgtctttga gtcattatcc aatccaagat tgtagtgatc
     4381 tcgtatttga gtggaaggca tttatgaaca ctatcgctga ttttgatgat gaaacattat
     4441 gctgtattgc taaaacatat gaagatgata cgcgtttcaa agattacttt aattcatatg
     4501 ataatcaaaa tttagcatca tacatttcag aagctgttaa ttattttttg agcaatgtga
     4561 ataagagcga caatttttaa tcctcataca gatgctacaa cactgaatac accaactaaa
     4621 ataattgttc aaagtagaaa actaatcatt tattctctaa atcaataact atattaaaag
     4681 ttataccttt gcaaagcgaa ttagtatagg ttaccgaaag gagaaaggat taggttccat
     4741 tcgatttata aaaaaatata tttcgaaaca tacaaaagcg ccagaagata atcatttcct
     4801 ctcgcgcttt tatactgaat tatattaatt tcaaactcta aaactctaat aatttctttt
     4861 tcaatgcata ttcaactagc tcaggtttac tctttaagcc aagctttgtc ataatatgcg
     4921 tcttatgtgc ttctactgtt ttcacagata caaataattt ctctgcaatt tctttattcc
     4981 cgtaaccttt ggcaataagt ggcaatattt ctagttctcg ttttgataag attttaaaag
     5041 gatctgttgt gtttgcagtg tcctgatttg aattagatac aaattcattc actaaagatg
     5101 ttgtaagttt catatctaca tatgtttcac ctttatatac agttcgaata gctaacaata
     5161 attgttcatc aggtgcattt ttcaatatgt aacctttcgc accattacgc aacacatgga
     5221 acaaatactc ctcatcatca aacattgtta atattagtat tttagtttca ggaaaactgt
     5281 cagcaatttt actcgtagcg ataagacctg actcacctgg tggcatactt aaatccatta
     5341 gtaacacatc aggtttatat tccattactt tttggtaagc ttcgacgcca tctgcagccg
     5401 ttgcaacaac ttccatatca ttttgataat ttaaaatcat agagaacccc gtacggacaa
     5461 cagcgtgatc atcggcaatg actattttca attttattcc cccaatgtat gtttcaaatt
     5521 ggaatgttca atgtaacatt ggtaccctca ccaattttcg tttcaatatt gacgctaccg
     5581 ctgactaact cagctcgctc attcattcca tataaaccga gtccagaacc tttaggctta
     5641 gaacttggat caaaaccatt tcccgcatct atcacttctg ctaccaaatg gcgcccagtt
     5701 tgacggatac ctacatttat ttcatttaca tcagcgtatt tcaacgcatt taaaatagct
     5761 tcttgcacta ctcgataaac aaccgtttca atatcactat caaagcgagt atttttaata
     5821 tttgatgtat atatgatttt tattccataa ttttcttcaa actgtttaaa atatgattta
     5881 aaagctgctt caaggcctag atcatccaaa gaagcgggtc ttaattcaac cgacatatta
     5941 cgtatatcat caattaattt agcgacaata tattcaatat tttctgcgtc ttccaaaagc
     6001 ttagttgtat cttcttgata ttttaataat ctcaattgaa catctacatt gagcatttct
     6061 tgaatcacac tatcatgtaa ctctctagaa attcgctttc tttcattttc ttgggctgag
     6121 attgttttac gcatcatacg ttgttgatgc aatttctctt gctgttcaat ttgtgatgaa
     6181 acattttgaa gcgtaaatgc atgaattccc ctgtcttgat caatcaactg atatgttgct
     6241 gtaaatggca tcactttttg atctttcgtc ttcataaata cttggaaatt cgtagcttgt
     6301 acttgcatcg attctaagaa acagtcttta catgattgta catcataggc atttgtatac
     6361 ccttcacatc tgtgacaaat cgcattcgcc acagcactgt aattatcttc ttcagataat
     6421 atatctttag cagcatcatt cattgcaata attttaccgt tatcatcagc aaaaactatc
     6481 ttttcgattg aatgctcata atattttttc aataaagtat ctaactgtat actgtcctca
     6541 ttaatcatga cttacaccct aattcatctc attatttatc atcattgaaa ataccaaact
     6601 tacgttgaat atcatcatta tcaaatattt ttggtaaagg acgaccatct ctttgaccaa
     6661 ataatagtac gccatacact tgattcttat accaaagcgg cactgctaaa actgctgtta
     6721 atgattcgct caataaaatt ggatagtcaa tcttttcttc aggccctaaa gctaaaccaa
     6781 cattggctat taccatacgc tttcctgttt tcataacagt tccagctaat ccacgacctt
     6841 ttcttaaaat aatcaattta aatcgattat ttttattacc tgaaacatag tgccatttta
     6901 ttggagatga tggtttgtta gattcataga aagcgattgc cgcaaaatca taaccctctt
     6961 ctttgcgtat tttatctaat gtctcttgaa atctacgatc ttcaattatt gcttctggtg
     7021 tcaaatcctt tcacctctta tgcttacact ttattcttac ggtaaataat atatctgcga
     7081 tttatatatg tcaaaggtac actccaaaca tgcaccaaac gtgtaaatgg ccaacaagcc
     7141 ataatagtga aacctaacaa tatatgcatt ttaaatgcaa tcggcacacc actcatcaat
     7201 gacgcatctg gttttaacat aaataattgt ctaaaccaaa ttgataatga agttctgtag
     7261 ttaaagtctg gatgttgtat atttgttact aatgttgcgt aacatcccat aaatacgata
     7321 agtaataata agaaatttac aaatatatcc gacgctgaac ttaatcttcg aatacttttc
     7381 gtagtaacac gtctcgctgt taataaaaac atccctatca aagttattat accaaagatg
     7441 ctaccaatat aaacagcgcc tatatgatat aaatgctcag acacacccac tgcatccatc
     7501 catggtttcg gtattaacaa tccaactacg tgtccaaaaa acactggaat aatacctaag
     7561 tgaaataata aacttcccca catcaacctt tttctttcta ttaattcact agatttagct
     7621 gtccaagaaa atttatcata acgataacgt gcaatatgac ctgcgacaaa gacaactaaa
     7681 cataaatacg gaaatataac ccataaaaac tgattaagca tgatgtttca ctccttttgg
     7741 tgatgtcaaa cataatttca atgtttttct aagtgcttga atcacatagg catatggatt
     7801 gttatcttca ccaagtgcat tcgccatcac atatgttcca tcctcaataa tcataatgat
     7861 taattgaata ttctcttcag ctcttggatc atttcgccat tctgccactt gcaaaaattg
     7921 aagcatcaac ggtagataat cagaaagttc attatctacc atttctagtc caaacatttc
     7981 atataatacc tttaatttag ctaacatttg cccacgttct ttttgcgtat caaatttgtt
     8041 atacgtcata tataatggtg cttttttcgt aaaatcaaat gtatctgtat aaatcgcttt
     8101 gatttctgat aatgaaaatg tcatcattac atttctaaat gctactaaat cttcatatgc
     8161 tggatgatct ttcgaaattg tttcttcaaa agtttttgga tgaaaagtta atttttctgg
     8221 aaaacataac tgttgtgcca tatatccaaa actttcttga tattttttaa aattatcgaa
     8281 attaatcacg gaaaatccct ccatagaaat tctcattata aatttcttga ccagttttcc
     8341 ctgaacctac tgcaacgcca cagccttcac agttatctcc aaaatgctcg ccgccgtaat
     8401 tgtatcctgt actaccttgt gcgtgatacg tatctaaata ggtttctttg tgtgatgttg
     8461 gaataacaaa tcgatcttca tatttggcta gtcctaataa acgatacatg tctttagttt
     8521 ggcgctcggt tatacctaat cgctctaatc gagacgtgtc aaatggctgt tgagtaactt
     8581 gagatctcat ataacttctc atcattgcca tacgttgtag ggctcctttt actggctctg
     8641 tatctcctgc agtgaaaata ttagctaagt attcaatagg taaacgcatt tcttcaatgg
     8701 ctgggaaaat cgcatctgga ttttgagttg tatttttacc ttcaaaatag ctcataattg
     8761 ggctaagtgg tgggcaatac caaaccatcg gcatcgttct aaattcagga tgtaacggaa
     8821 atgcaagttt atattcaatt gctaacttat aaattggaga gttttgtgca gcttcaatcc
     8881 aatcgtaacc aataccatct ttttcagctt gagcaatgac ttcttcgtca aatgggttta
     8941 agaatatatc taattgtttt tcatataaat ctttctcgtc tactgctgaa gctgcttcat
     9001 gaactcgatc tgcatcatat aataaaacac ctaagtaacg catacgtcct gtacaagttt
     9061 cagagcatac cgtaggcata cccgcctcga ttctcgggaa acagaaagta cacttttcag
     9121 ctttgttcgt tttccaattg aagtaaactt tcttatatgg acaacctgtc atacagtaac
     9181 gccatccacg acatgcgtct tggtcaacta atacaatgcc atcttcatca cgtttataca
     9241 tagcacctga aggacacgat gcaacgcaac ttggattcaa gcaatgttca cataaacgtg
     9301 gtaaatacat cataaaagtt tcgtcaaatt ggaatttaat atcttcttct attttttgga
     9361 tgttaggatc ttttggacct gtaacatgac cacctgctaa gtcatcttcc cagttaggtc
     9421 cccattcaat ttcaatgtta tcccccgtaa tttctgaata cgctctagca actggcgaat
     9481 gcttccctga tttcgcagtt gttaaatgtt cataattata gttccatggc tcataataat
     9541 ctttaattaa tggcatatct gggttataaa aaattttacc taaagcaatt tttgaaattc
     9601 tacttccaga ttttaattca agtttccctt tacgatttag tacccaacca cctttgtagt
     9661 gttcttggtc ttcccaacgt ttcggatacc ctacacctgg cttcgtttct acgttgttga
     9721 accacatgta ctcagcacct ggacgatttg tccaagtgtt tttacatgtc acactacacg
     9781 tatggcatcc tatgcattta tctaaattta ataccatcgc aacttgcgct ttaatcttca
     9841 agccaattaa cctccttcat ctttctaact gctacatata aatccctttg gttcccaatt
     9901 ggtccataat aattaaagtg ataactaatt tgtgcgtatc ctccgactag ttgtgttggt
     9961 ttcaaatgga ttctagtcgg cgcgttgtgt gaaccaccac gtgtatctgt aatttctgac
    10021 ccaggcgttt gaatatgttt atcttgtgca tgatacataa acattgtacc tttaggcata
    10081 cgatgcgaaa taactgctct tgccgttaca acaccattac ggttatacac ttctagccaa
    10141 tcattatctt ggatatcgtg tttttcagca tcttcatttg atatccaaac cgttggacca
    10201 cctctaaata gtgtcaacat atgcttatta tcttgataca ttgagtgtat attccatttt
    10261 ccatgaggcg ttaaataacg cagtaccaaa gcatctgtac cacctttaat tttcttatct
    10321 ctattcccaa ataccattgg cggcaatgtc ggtttatata ctggtaagct ctccccaaat
    10381 tgttggaaaa cttcgtgatc cacataataa ctttgacgtc ctgttaatgt tctaaaaggt
    10441 actagacgtt ctatattcgt tgtaaatggt gaatatcgtc gaccttgttt atttgaacct
    10501 gggaatactg ctgtcggtat tacttctcgt ggttgtgaag ttatatttaa aaacgaaatt
    10561 ttctcagcag cgcgttcgct agaaatatct tttaacggca ttccagtttg ttcttcgaga
    10621 tcttcatatg atttttgtga taatttacca ttcgtagcag atgaaatact tagtattgca
    10681 tcagctacat tacgtgctgt atcaatacgt ggacgattcg ctctcacaga atcatcattt
    10741 gtatcactcc acgtacctaa catacttttt aattcttcat attgttcact gacaccgaaa
    10801 cttacaccat gtgctccaac tttccctttt tcaagtacag gaccaagcgt gacatatttg
    10861 tcgtaaattt tagtgtagtc gcgttctaca attgcaaagt taggcattgt acgtccaggt
    10921 accgcttcaa tttcaccctt cgaccaatct ttcactacgc cgtatggtgt tgaaatttct
    10981 tgctttgtat catgactaag tggagttgtc acaacatctt taaacgttcc aggtaaatag
    11041 tcttttgcca tttctgaaaa tgcttttgcc aacgttttat aaatatccca gtctgaacgc
    11101 gattcccata acggatcaat ggcaggattg aaaggatgta catatggatg catatccgtt
    11161 gatgataaat catgtttttc ataccaagtc gctgccggca aaacaatgtc agaatataac
    11221 ggtgttgccg tcattctgaa gtctaaagag accactaaat ctaacttacc tgttgtttct
    11281 tcacgccacg taatttcttc tggcttttca tcttcatttg gtgtagctaa taaccctgat
    11341 tttgtgccaa gtaaatgctt cataaagtat tcttgacctt ttgcagaact tgaaattaag
    11401 tttgaacgcc atataaataa tgattttgga tgattctttt tcaaatcagg atcttctatt
    11461 gcaaattgtg tttgttttga tttcacttca tcaattgcac gttgcaaaat cgcttcattt
    11521 gaatctatac cttcatcttt agcttcttct gcaaacaaca aactattttt attaaattgt
    11581 ggatatgatg gtaaccaacc aagtctagct gctaaaacat tataatcagc tggatgttga
    11641 tgctttaact cctctgtttt agctaatgga gattttaaac gatctacatt tgactcttca
    11701 tatttccatt ggtctgttgc aaaatagaac caacttgttc cgttttgcaa acgtggtggt
    11761 ccttgccagt ctttcgcaaa tgcgacagta ctccatcctt caatcggacg acatttttct
    11821 tgtcccacat agtgagccca accgccacca ttcacacctt gacagccaca taacataact
    11881 aagtttaaga ttgaacgata aatcgtatct gagttaaacc aatggttaat acccgcaccc
    11941 atgataatca ttgaacgccc ttcagtatcg atagcgtttt gcgcaaattc tttcgctact
    12001 tgaatgacaa cactttgttt tacgcctgaa atggcttctt gccaagcagg tgtatatttt
    12061 gattctgcat cgtcgtatcc ttttgattct aatttatgat caaaacgacg cacgccatat
    12121 tgacttgcca ttaagtcaaa aattgtagca atacggactt tgtcaccatt tgctaaagtg
    12181 acttgtcgag ttggaattgg acgattgaat atcccatctc catcactatc aaagtatggg
    12241 aattgaattg tttctaattc gtatccacct tctgtcattg ataatgtagg gttaatttta
    12301 gaaccatctt ctgtttctag ttttaagttc cacttcttac cttcttccca acgttgaccc
    12361 attgtgccat taggtactac taaactatcg ctgattgcat catgaataac tggcttccat
    12421 tcgccttgct ctgttgtttg acctaagtca ctcgctctta aaaatcgacc cgctttatat
    12481 ccattttcat cttcatccag catgataaga aacggcatat ctgtatattg tttagcgtaa
    12541 tttataaagc gttcattagg ttgattaaca taatgttctt gtaaaataac atgcgtcatt
    12601 gcttgtgcaa ttgcagcatc tgaaccagga ttcggtgcta gccagttatc tgcaaatttc
    12661 acattttctg cgtaatctgg tgctactgaa atgacttttg tacctttata gcggacttca
    12721 gtcataaaat gtgcatccgg agtacgtgtt aaaggtacat tagagcccca cataataatg
    12781 tatgatgcgt tataccagtc acttgattca ggcacatctg tttgctctcc ccaaatttgt
    12841 ggagaggcag gtggtaaatc tgcataccag tcataaaaac taagcatttc accaccaagc
    12901 aaattgatga atcgagcacc tgctgcataa ctaatcattg acatcgctgg aataggtgta
    12961 aatcctgcga ttcgatctgg accatatttt tttattgtat acagtaattg tgctgcgatt
    13021 atctctgtaa cgtctttcca atttgaacgc acgtgccctc ccatacctcg ggcttgctta
    13081 tattgtttgg ctttgtcttc attttcaaca atagacgccc atgcagcaac gcgattacca
    13141 ttgttttctt ctaatgcttc agtccataaa tcccagagtt ttccacgaat atatggatat
    13201 ttgattcgaa gcggactgta ttcataccaa gagaatgacg cacctcgtgg acatcctctc
    13261 ggttcatatt caggcatatc cggaccacaa cttggatagt cagtttgttg attttcccag
    13321 gtaatcacac catttttcac aaatactttc caagaacatg agcctgtaca gttaacacca
    13381 tgtgttgttc ttacttcttt atcgtggctc caacgttctc tgtacatttt ttcccattct
    13441 ctacttttac tttctaggat cgaccaattc ccattaaatt tttctgttgg cttaaagaaa
    13501 ttcaatccaa attttcccat atttatatcc tcctacgtat aaaaatacga tgtgtagatg
    13561 tcgtgttttt aaatacttta aaatgcccaa gactattgct ttaattagat tgtacatttt
    13621 ttcacaaaca taaaatatta gggaatcacc taattactta aggaatttcc ctatcaataa
    13681 cgggatttca ttgaaataat acacaatcat gtatggtcat gcttattgcc aatctaaatc
    13741 gttcaaattt ggcacaacga caaataaggc ttcaacacga atatattctc tcggttgaaa
    13801 ccttacttat tcatttattt tttataaatt agtgacataa cactgtatta gcatctgcac
    13861 gatcggttga aatatatgtt acattttctt gctgcttaat aaatgcatca tagtaatcat
    13921 attgcgacga atgatatgtg ccattcgatg tatcatttgg gtttagcaaa cagccataac
    13981 cttcgtcata taaatgttca cagagcataa gggcgtcatg tttagaacca cttactacat
    14041 aaaattgctt cataggatca tatgatttag gagtgttttc agtataatca acaacttccc
    14101 ctataataca tatacctggt ttcgcctcaa ttgaatagtg ttgcaatttt gaaataatat
    14161 tacttaaacg ccccttaaca acaaactcgt taaaacacga tgcttgaaag acaatcgcta
    14221 tcgggtaatc aatatctgtg tattgttgta tctgtgtgat aattttccct aaacgtttta
    14281 cccccatata aattgctaac gtgccaccat tcactaagga attgacatcc acttcatttt
    14341 cttctgaatc tttaaagtga cctgtagaaa atgtcacact tttagcaact gtacgcattg
    14401 tcaaacctgt ctgcatagta gcaactgctg cgctcgctga tgtcacccct ggtacaattt
    14461 caaacgcaat atgatgttca tttagtgtgt cgacttcttc ttgcacacga ccaaatatcg
    14521 ctggatcgcc acctttaagt ctaacaacct tgttatatcg acgcgctgct tccacgatac
    14581 agtcatttat tttttcttgc tgaatatgtt ttgcatacgg ctttttacca acatcgataa
    14641 tttcagtagt caaattcgca tattgtaaaa ttaacggatt cactaatcga tcatatagaa
    14701 tgacatccgc ttcacgtatt aaacgctcag cctttttcgt caaataattc ggattacctg
    14761 gacccgcacc tatcaagtaa accttgccat attcctctac agacatatat atacgttccc
    14821 gtctgtaact tctacctcat aaacatctac acaaccttca tcaggttctt gaacaatacc
    14881 tgtatttaaa tcaatttttt gatcgtggag cgggcaaaat acatattccc cactcactgt
    14941 cccttcagac aatggtcctt gtttgtgtgg acagatattg tgaatcgcat gaattttgcc
    15001 actttctgtt aaaaacaacc ctacctcttt gcctttgaca ataacctttt ttccaattag
    15061 gggtgttaat tcatctatag ttgtcacttt aattttttct tttgtttcca tgtattacac
    15121 cttctccact tcaaaaattc tacgtgcttg cgcattgcta gttattgctt cccaaggttc
    15181 agcttcgact gcttttttag catccataat gcgttcaaat agttcatttt gtctttctgg
    15241 gtcaagtaag acttctttta cattttcaaa tccaagtctt cttaaccatg gcgctgttct
    15301 ttcagcatat atacctgttt cgcgataata ttgcatcaaa gcaccacata atttgattac
    15361 ttcatcttct gtttctacag ttgttaaaaa ttcagctttt tcaacttctg taccaccatt
    15421 accaccgata tagatttgga atccattttc aactgagata ataccaaaat ctttaacacc
    15481 tgattcaaca caacttcttg ggcagcctga tacacccatt ttgaatttat gaggtgtatc
    15541 gatgtattca aatgtttttt ctaaacgaat gccaagtcgt gtcgtgtatt gcgtaccaaa
    15601 tcgacaaaac tctttaccaa cacagctttt aactgagcgt gttttcttac cataagctga
    15661 tgctgaacgc atacctaggt cttcccatat atttggtaat tcttcttttt taactccata
    15721 caaaccaaca cgttgtgaac ctgtcacttt aactagtggc acatgatatt tcttagccac
    15781 ttctcctaga cgaatcagtt ggtctgcatc tgtaacaccc ccacgcattt gaggtataac
    15841 agaaaatgta ccatcatttt gaatattcgc atggtaacgt tcgttagcaa atcttgattc
    15901 tctttcatct tcatgatcat gtggataaac catgtttaaa taatagttga ttgctggtcg
    15961 acattttgga catccacctt tatttttaaa gtttaaaaca tgtcgaactt ctttagatgt
    16021 ttttaaacct ttcgctctta tttgcgttac tatttgatcg cgtgtcaaat cagtacaacc
    16081 acatatacca gcaggttttg cggcaacaaa gtcatctcct aaggtgtgct gcaatatttg
    16141 agcaatttgc ggtttacatt taccacatga attccccgct tttgttttag ccgttacttc
    16201 ttcaactgtt gtaaagccat tttccgtaat cgcatttact atagtacctt tatcaacacc
    16261 attacaacca caaattgttt catcatcagc catatcagca attgatagcg atgcctcttc
    16321 tccaccttta gtaagcaatg atacaagtgt gtaatcttca gtggattcac cttttttcat
    16381 catgttataa aagcgtgaac catcatcgat atcaccatat agtactgcac caactacatt
    16441 accgtctttt aaaaagattt ttttatagtt attatcaaca ctattaaata tttcaatacc
    16501 tttaatttct gcattttcta caatttgacc agcactatac aagtcacacc cagaaacttt
    16561 taatgacgta aatgttgttg atcccttgta tccgttcgtt tctttatttg ttaaatgatc
    16621 agctaatact ttaccttgtt catatagtgg tgcaacgagt ccataaactt tgccgttatg
    16681 ttctgcacat tcaccaactg catatacatt gctatcactt gtttgcatca catcattgac
    16741 aacaatacca cgattaacat ctagacctga ttctttggct acttctgtgt atggtcgtat
    16801 acctactgcc ataacaacta agtctgccgg aatctcgcgt ccatcagcca atttaacacc
    16861 ctcaacatca tcttctccta agatttcagt tgtgttggct tgcatttcaa acttcatacc
    16921 ttgcttttct agatctgctt taagcatatt tccagcttta cggtctagtt gcatttccat
    16981 caaccattca gctaaatgta acaccgttac ttccatacct tgatctaata aaccacgtgc
    17041 acactctaaa cctagtaatc ctccaccaat tacaattgct ttctttttag tcttagcaat
    17101 gttcatcatt tgttcagtat catcaatcgt tctccaacca attacactcg gtaatgtcga
    17161 acctggaact ggaatgacga atgctttaga acctgtcgca aaaatcaatt tatcgtatga
    17221 tacttcaata ccatttgcag tagtaactga ttgatttgct ctatctactt caattacagg
    17281 atcatttgta attaactcga taccatgttc ctcataccac tcatatggat tcataattgt
    17341 ttcttcaact gtcattttat tttgtaaaat atttgaaagc atgatgcggt tatagtttgg
    17401 ataaggttct ttacctatta ccgtaatatc atataaatcg ttggcgcgct ctaatatttc
    17461 ttcgattgtt cgaatgcccg ccataccgtt accaatcatt actagttttt gctttgccat
    17521 aaaatatgcc cctttactcc ataatattta tttcaaaaaa aaggtattaa tttttcgtta
    17581 gtgcttttat attttcattg gaatcattaa gctttctaat ctatcgttaa tgatttgctt
    17641 taaaattggg tcgaagttaa ttgaaggtgt gaagtgtata tctgtattaa taaccatgtc
    17701 attcatttgc tgcttcactt tgttaacaag tcttccgtca tataaaaata atggtacgac
    17761 aatcaatttt tgataccgtt tcgagatgct ttctaaatca tgtgtaaaac taatctctcc
    17821 atatagcgtt ctcgcatatg tcggcttgct aatttgcaaa ttttgagcgc atatttgtaa
    17881 ctcttcgtgt gccttagtaa actttccatt aatattgccg tgtgcaacaa ccataactcc
    17941 aacttgttgt tcgtcacctg ctaatgcgtc acaaatacgt tgttcaatta atcgtctcat
    18001 taaaggatgt gtgccaagtg gctcgcttac ttctaccttt atgtctggat accgtcgttt
    18061 catttcatga acgatattcg gtatatcctt gagataatgc attgcactaa agattagcaa
    18121 tggtacaatt ttaaaatggt caaccccact ttgaatcaac gtcgtcatta ccgtctctaa
    18181 atcctgatgc tcactttcta aaaacgcaat atcatagtga tgtatatcat cttttactaa
    18241 ttcagaaata aatgcttcta acgcttgatt ctgtcgtccg tgcctcatgc catgtgcaac
    18301 aatgatattc ccattcacat ttaccaaccc tttcacacgt attgtatacc aaatcatttt
    18361 gtttttgtga aaagaatcac attataatgt aaaatcaggg aattccctga tgcctgtagt
    18421 catgcatatt ccttatacat tttccctttt tgttaaatca aaaaaagcga ccgatatatg
    18481 aatccctact caacatttat ttgagcaagc attaatatat cggtcgcttg tagtgtatat
    18541 tattatctta aaatggtggt tggcctaata ttgtttcgtc aaagcgctcg ggtatcaata
    18601 ctttgcgcat gatcacacct aaatcgccat catcattttc atgttcgctg tatatttcat
    18661 aacctctttt ttcataaatt ttaagtaacc acggatgcaa tcttgcagat gtacctaaag
    18721 taactgccgc tgactttaac gtatctcgca aaaatgcttc ttcaacataa gtaagtaatt
    18781 ggctaccata gcctttccct tcatactcag gatttgtcgc aaaccaccag acaaaaggat
    18841 aacccgaaat acttttcaca cttccccaag gatatctaac cgtaatcgta gatataattc
    18901 catcatcaat tgtcatgaca aatgtagtat ttttatctat attttcttta acagcatcta
    18961 aattagcatt aaccgaaggc caatcaatac ctagttctct tagaggcgta aatgcttcat
    19021 gcatgagttg ttgcaatttt tctgcatctt gttcacttgc gagtcgaatc atcgtttttg
    19081 tcatattaat ccccactctt ttttaaatga tttaaccata ttttattttt aaaataaata
    19141 tccatcaaag tgtatcaata aatttatcac atgtcagaaa gtatgcttca tctgaataca
    19201 ccaatactct catgaaactt attaaaaatt actctctcaa cgtaaaaaac cattcaaatt
    19261 catgaatggt ttggaagaat gattcattgt tacgctattt aatcactaca tcttaattat
    19321 tgttgctcta aacgattacg cttaccattt aagaaagcat aaacgagacc tacaaaaata
    19381 ccgccaccga caaagttacc taagaaagca aaaacgatat tttttaaaac gtgtaaccat
    19441 gaaactgcat caaggttaaa gaataccata cctgcatata gacctgcatt gaacacaacg
    19501 tgctcatatc ccatgtatac aaagaccacg acaccacaag ctatgaagaa tgcctttgtt
    19561 aagccgcctt tgaattgcat agagatgaaa ataccaatat taataaagaa gttacagaaa
    19621 ataccttttg taaaaatatt caaccatgtt gaatcaacag tctttttctg aactaaagct
    19681 gttaaagctt gtgtcatttc aggtgtcatg acatgcgcaa atttcattaa gaaaaataac
    19741 acaaacccac ctaaaatatt tcctaagaaa caatataata aaatccaagt catcttctta
    19801 acagaaacga ctttataata ccagcctact gtaaagtaca tgaagttact tgttaataat
    19861 tcagagttag ttaatacaac taaaatcaaa cctaaactaa acgcaatggc tcccattaaa
    19921 ttgataagtc catctacgtg agtactcgca aactgcgtct ttattccgaa cataaataca
    19981 gtaacgattg agagtaaaaa tccggccatc attgctttaa gcatatatcg gccaggtgtt
    20041 tgctgtacca ttacttgctt catttgtact gaagatgata ttgaatcaac aattgcgcct
    20101 gttgaatatg tatcttcaac tgttttctta ttttctatca cagtacttcc cccatttact
    20161 ttgatcaaat aaaatttact ttctaattat attagtaaat caatgtaatt gatagattaa
    20221 atttcagaaa aatcttttta ttctctcgaa aatgtagttt tctttcataa aaacagcgag
    20281 ataaatcgcg tgattggcta atatctttat gatttaagat attacgacgc tcattttatc
    20341 tcgctttatg taaagtatta cttattcttt tttaaatttg aaaagtaatg taaaccatat
    20401 gcaaatacac tgtaaccaat tgcagtacgt aaaataaacc ttataacttt attaggtaca
    20461 cattttgacg ttactgaaat agaagata
//
LOCUS       DAXWFK010000030        20485 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.030, whole genome shotgun sequence.
ACCESSION   DAXWFK010000030 DAXWFK010000000
VERSION     DAXWFK010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20485
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.030"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            218..1273
                     /gene="mupG"
                     /locus_tag="ACGEXS_000876"
     CDS             218..1273
                     /gene="mupG"
                     /locus_tag="ACGEXS_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-N-acetylmuramidase"
                     /protein_id="HFF8011909.1"
                     /translation="MTGFSVYLGQPLDEAYIKRMIKQGYQMIFTSVQIPEEDDETKYH
                     YFTKLLNLLKHEQVTYLIDANPSILTPSFYDHLRQYDAQFMIRIDHSTSIEAIEAIMA
                     QGLKCCLNASIISRELLTSLHQQLNDFTLLSFCHNYYPRPDTGLSVDLVNKKNELIYQ
                     FNPKAQIYGFIVGSDLRGPLHKGLPTIEATRHSHPVVAAKLLQETGVSEVLVGDSLIE
                     MRQAKQLIDFCKHRHFTLCIEEVFDTTVTYLFDMCHKVRPDNPENVIRSETSRQICPH
                     SIQPQFTTQRRIGSVTVDNLNNGRYQGEMQIVRQTLSAHDNVNVVAQIIKEDLPLLSC
                     IEPNDTFDFQKTRECKR"
     gene            1273..2169
                     /gene="murQ"
                     /locus_tag="ACGEXS_000877"
     CDS             1273..2169
                     /gene="murQ"
                     /locus_tag="ACGEXS_000877"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFF8011910.1"
                     /translation="MENSTTEARNEATMHLDEMTVEEALITMNKEDQQVPLAVRKAIP
                     QLTKVIKKTIAQYKKGGRLIYIGAGTSGRLGVLDAAECVPTFNTDPHEIIGIIAGGQH
                     AMTMAVEGAEDHKKLAEEDLKNIDLTSKDVVIGIAASGKTPYVIGGLTFANTIGATTV
                     SISCNEHAVISEIAQYPVEVKVGPEVLTGSTRLKSGTAQKLILNMISTITMVGVGKVY
                     DNLMIDVKATNQKLIDRSVRIIQEICAITYDEAMALYQVSEHDVKVATVMGMCGISKE
                     EATRRLLNNGDIVKRAIRDRQP"
     gene            2181..3635
                     /locus_tag="ACGEXS_000878"
     CDS             2181..3635
                     /locus_tag="ACGEXS_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFF8011911.1"
                     /translation="MTKEQQLAERIIAAVGGMDNIDSVMNCMTRVRIKVLDENKVDDQ
                     ELRHIDGVMGVIHDERIQVVVGPGTVNKVANHMAELSGVKLGDPIPHHHNDSEKMDYK
                     SYAADKAKANKEAHKAKQKNGKLNKVLKSIANIFIPLIPAFIGAGLIGGIAAVLSNLM
                     VAGYISGAWITQLITVFNVIKDGMLAYLAIFTGINAAKEFGATPGLGGVIGGTTLLTG
                     IAGKNILMNVFTGEPLQPGQGGIIGVIFAVWILSIVEKRLHKIVPNAIDIIVTPTIAL
                     LIVGLLTIFIFMPLAGFVSDSLVSVVNGIISIGGVFSGFIIGASFLPLVMLGLHHIFT
                     PIHIEMINQSGATYLLPIAAMAGAGQVGAALALWVRCKRNTTLRNTLKGALPVGFLGI
                     GEPLIYGVTLPLGRPFLTACIGGGIGGAVIGGIGHIGAKAIGPSGVSLLPLISDNMYL
                     GYIAGLLAAYAGGFVCTYLFGTTKAMRQTDLLGD"
     gene            3638..4513
                     /locus_tag="ACGEXS_000879"
     CDS             3638..4513
                     /locus_tag="ACGEXS_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFF8011912.1"
                     /translation="MTNILYRIDKQLSDFTKTEKIIADYILKNPHKIIDMTVNDLADV
                     TNVSTASIVRFSRKMTHQGFQELKIAISRYLPEDIATNPHLELIENESVETLKNKMIA
                     RATNTMRFVATNIMDAQIDAICDVLKNARTIFLFGFGASSLTIGDLFQKLSRIGLNVR
                     LLHETHLLVSTFATHDDRDCMIFVTNQGSHSELQSIAQVATHYSIPIITISSTANNPV
                     AQIADYALIYGRTDENEMRMAATTSLFAQLFTVDILYYRFVALNYHAILDCITQSKMA
                     LDNYRKHLATIDFKH"
     gene            complement(4707..4988)
                     /locus_tag="ACGEXS_000880"
     CDS             complement(4707..4988)
                     /locus_tag="ACGEXS_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011913.1"
                     /translation="MVLITYQIILFFIISLSYYLTLNHYMAVTVGNFTSIFGMFAAIL
                     FMYYYLLYKSPEYNQRKRFKHFIHITNLIIIAFSTFVLVHLALKLFFSI"
     gene            5201..7990
                     /locus_tag="ACGEXS_000881"
     CDS             5201..7990
                     /locus_tag="ACGEXS_000881"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFF8011914.1"
                     /translation="MAYQSEYALENEMMNQLEQLGYERVTIRDNKQLLDNFRTILNER
                     HADKLEGNPLTDKEFQRLLTMIDGKSIFESARILRDKLPLRRDDESEIYLSFLDTKSW
                     CKNKFQVTNQVSVEDTYKARYDVTILINGLPLVQVELKRRGIDINEAFNQVKRYRKQN
                     YTGLFRYIQMFIISNGVETRYFSNNDSELLKSHMFYWSDKQNNRINTLQSFAESFMRP
                     CQLAKMISRYMIINETDRILMAMRPYQVYAVEALIQQATETGNNGYVWHTTGSGKTLT
                     SFKASQILSQQDDIKKVIFLVDRKDLDSQTEEEFNKFAKGAVDKTFNTSQLVRQLNDK
                     SLPLIVTTIQKMAKAIQGNAPLLEQYKTNKVVFIIDECHRSQFGDMHRLVKQHFKNAQ
                     YFGFTGTPRFPENSSQDGRTTADIFGRCLHTYLIRDAIHDGNVLGFSVDYINTFKNKA
                     LKAEDNSMVEAIDTEEVWLADKRVELVTRHIINNHDKYTRNRQYSSIFTVQSIHALIK
                     YYETFKRLNKKLEQPLTIAGIFTFKPNEDDRDGEVPYHSREKLEIMISDYNKKFETNF
                     STDTTNEYFNHISKNVKKGVKDSKIDILIVVNMFLTGFDSKVLNTLYVDKNLMYHDLI
                     QAYSRTNRVEKESKPFGKIVNYRDLKKETDDALRVFSQTNDTDTILMRSYEEYKKEFM
                     DAYRELKMIVPTPHMVDDIQDEEELKRFVEAYRLLAKIILRLKAFDEFEFTIDEIGMD
                     EQENEDYKSKYLAVYDQVKRATAEKNKVSILNDIDFEIEMMRNDTINVNYIMNILRQI
                     DLEDKAEQRRNQEQIRRILDHADDPTLRLKRDLIREFIDNVVPSLNKDDDIDQEYVNF
                     ESIKKEAEFKGFAGERSIDEQALKTISNDYQYSGVVNPHHLKKMIGDLPLKEKRKARK
                     AIESFVAETTEKYGV"
     gene            8195..9037
                     /locus_tag="ACGEXS_000882"
     CDS             8195..9037
                     /locus_tag="ACGEXS_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7726 family protein"
                     /protein_id="HFF8011915.1"
                     /translation="MDNRNMINRVFSQKILHQIAIKNKSDVVDEAYDFYIQGPKNINV
                     IQKMKSLYNYLKKSYRNEYFYKNTILNKLLLGRHSINTTTALSEMPIGKSIADFILLN
                     GKGVVYEIKTELDKLDRLDNQINDYYEVFNYVVVITNDKHLNKVMARYKDTTVGILVL
                     TTRNTLSEVQKPKENNSLLNTKAMYNFLRKEERKRVIAQNHMDVPTYNDFTEYDVLFD
                     VFKEIPMTKLHNNMISELKKRGNMKEYKDEFLAAPAEIKFLLYFAKMTKKDKNKLYHF
                     LKED"
     gene            9039..9962
                     /locus_tag="ACGEXS_000883"
     CDS             9039..9962
                     /locus_tag="ACGEXS_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFF8011916.1"
                     /translation="MYYPYLRGKQNELFAIRELLEKGLIGDCIQPIIEPIKYTTTFKN
                     TLQYCGEKAFSINLVVNSKLTEEEISNETVAHLTEIITKNKSVIQKAYLGPSDEGNDR
                     LKQQFSSNSLAILTSVDDWEMFGDKNKLEMVFVPDDRHIKRKLRNIPNKGIIMDPFNK
                     LSRNVDYLDNDDEFYSDDHLYYKEDGYVAFSDYSVIGGEYVDGGFSPLAIAIHIVYFD
                     EANELRVKHFVSDSNNDRSNPGKKFFEAVDKLVTWSKNLDIKNRSYALGQFEELNENN
                     KYPGLGLIKRLSIMHHLEIMNRYLESQNENM"
     gene            9949..11061
                     /locus_tag="ACGEXS_000884"
                     /pseudo
     CDS             9949..11061
                     /locus_tag="ACGEXS_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498763.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RES domain-containing protein"
     gene            complement(11326..12918)
                     /locus_tag="ACGEXS_000885"
     CDS             complement(11326..12918)
                     /locus_tag="ACGEXS_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8011917.1"
                     /translation="MSNLLEVNSLNVQFNYDETTVQAVKNVSFELRKKHILGIVGESG
                     SGKSITAKSILGLLPDYPDHTLTGEIIFNGQSLNNLSTSALQQIRGKDISMIFQDPLS
                     SLNPRLTIGKQITEVIFQHKRVSKSEAKSMTIDILEKVGIKHATRQFDAYPHELSGGM
                     RQRVMIAMALILKPQILIADEPTTALDASTQNQLLQLMKSLYEYTETSIIFITHDLGA
                     VYQFCDDVIVMKDGSVVESGTVESIFKSPQHTYTKRLIDAIPDIHQTRPPRPLNNDIL
                     LKFDRVSVDYTSPSGSLYRAVNDINLAIRKGETLGIVGESGSGKSTLAKTVVGLKEVS
                     EGFIWYNELPLSLFKDDELKSLRQEIQMIFQDPFASINPRFKVIDVIKRPLIIHGKVK
                     DNDDIIKTVVSLLEKVGLDQTFLYRYPHELSGGQRQRVSIARALAVEPKVIVCDEAVS
                     ALDVSIQKDIIELLKQLQLDFGITYLFITHDMGVINEICDRVAVMKNGEIVELNNTED
                     IIKHPQSDYAKQLISEVAVIAK"
     gene            12875..14353
                     /locus_tag="ACGEXS_000886"
     CDS             12875..14353
                     /locus_tag="ACGEXS_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8011918.1"
                     /translation="MNCTFRLLTSNKFDMMLCLPIHKCVNLFYTFVYHSNLLKIILQG
                     VIIIITYLESFKSLYHKAFYKFVLSVLSLPIFLYAVIKFFFSAKRKNFYANNSEISEI
                     EQALHQKYKYLSQQKSSTQIHKEALKIFKAQSSNTSSKNIEQAHFSTYFENVLFHKFI
                     MIKVILALPMFILLTFYLQPLVRYIFERIVMAVIVIIGVIVSVFTILYFSPLDAAYSI
                     LGQNATKAQIHQFNVLHHLNEPYFIQLWDTIKGVFTFDLGTTYKGNEVVTKAVGERIP
                     ITIIVAVLALMVALIIAIPIGIISAMKRNSWLDITLMIIALIGLSIPSFWQGLLFILA
                     FSLKLDILPPSYMPEHPISLILPVLVIGTSIAASITRMTRSSVLEVMRSDYVLTAYAK
                     GLSTTQVVIKHILKNAIIPIVTLVGLLVAELLGGSAVTEQVFNINGIGRYIVQKQLIP
                     DIPAVMGGVVYISIVISLANLIIDIFYALIDPKLRSEINERK"
     gene            14359..15522
                     /locus_tag="ACGEXS_000887"
     CDS             14359..15522
                     /locus_tag="ACGEXS_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8011919.1"
                     /translation="MAQLNSKIASLKLFASYAIATYILVILTSALNLFKGYVADTFYI
                     AETLLIVLTIILIIILTTEQTWKHHDLWRRIVEVLLLLMTLTGNVFTLLMFVSIRRYQ
                     RTSQIHSYNGWESFIRKTTRHRIAIIGLLILVYMLTLSIVSQFTFDTTLATKNQFNAL
                     LHGPSLAYPFGTDDFGRDLFTRVVVGTKLTFSISIISVVIAVIFGVLLGTIAGYFNHI
                     DNLIMRILDVVFAIPSLLLAVAIIASFGASIPNLIIALSIGNIPSFARTMRASVLEIK
                     RMEYVDAARITGENTWNIIWRYILPNAIAPMIVRFSLNIGVVVLTTSSLSFLGLGVAP
                     DVAEWGNILRTGSNYLETHSNLAIVPGVCIMFVVLAFNFIGDAVRDALDPRIH"
     gene            15539..17314
                     /locus_tag="ACGEXS_000888"
     CDS             15539..17314
                     /locus_tag="ACGEXS_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8011920.1"
                     /translation="MKKIISIAIIVLALVLSGCGVPTKSEVAQKSSKVEVKGERPTIH
                     FLGQASYENDMNIVKDQLENAGFNVKMNIQPDYGSYRTQRQAGNYDIQIDDWMTVFGD
                     PNYAMTALFSSTGSNSLLKDKHVDQLLNKASTQNEADVKQTYKQIEDEVVFDKGYMAP
                     LYGSKKNLVYDNKVLDKNSVGLPNSRALIWQQFDYNNSRERDTRPLVMTQQDGEIPTL
                     DPIRSIAPSVYSINMNMYTRLLLLDENDHLTTKGSLSHDYAVNKDNKAFYFLLRDDDY
                     FAKVVNGQARNTGERVSAEDVKFSLDRARDKKSVPNNNTYNMHKHINDIKILKDEDID
                     QLRKEKDKDDKSIYDKLIKAYNVKSLTTDGQKVNNKDGIYQIVKITTDQSMPREVNYL
                     THSSAGILSKKFVNQVNQEYPKGYGDSSTIPANSDGKNALYASGAYIMTQKNAYQATF
                     QRNPGFNETEKGSYGPAKIKNITLKFNGDPNNALSELRNHSIDMLADVNQKHFDLIKS
                     DKNLSIIRKNGRKSVFLMLNIKKGIFKTHPNLRQAVVNAIDQDQFIKFYRGDKFKIAS
                     PITPLVDTGNEQRQDLEKVEKAINQ"
     gene            17352..19358
                     /gene="ggt"
                     /locus_tag="ACGEXS_000889"
     CDS             17352..19358
                     /gene="ggt"
                     /locus_tag="ACGEXS_000889"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFF8011921.1"
                     /translation="MVINLNDKQTKTSKEGLISVSHPLAAKIGKDVLDQGGNAMDAVI
                     AIQLALNVVEPFASGIGGGGYLLYYEQSTGSITAFDARETAPEHVDKQFYLDDSGEYK
                     SFFDMTTHGKTVAVPAIPKLFDYIHKRYAKLSLEDLINPAIELAIEGHAANWATEKYS
                     RQQHARLTKYHETAQVFTHENQYWREGDWIVQPELGKTFQILREQGFNAFYKGDIAKQ
                     LVNVVKACGGTITLEDLAKYDIQIKAPISATFKDYDIYSMGPSSSGGITVIQILKLLE
                     HVDLPSMGPRSVDYLHHLIQAMHLAYSDRAQYLADDNFHEVPVQSLIDDDYLKARSTL
                     IDSNKANIDIEHGVVSDCISHTDVEENHTETTHFCVIDKEGNIASFTTSIGMIYGSGI
                     TIPGYGVLLNTTMDGFDVVDGGINEIAPYKRPLSNMAPTIVMYHGKPILTVGAPGAIS
                     IIASVAQTLINVLVFGMDIQQAIDEPRIYSSHPNRIEWEPQFSQSTILALIAHGHAME
                     HKPDAYIGDVHGLQVDPTTYEASGGSDDTREGTVMGGEVLVIRKQPLPYRQMYDSDGF
                     RLYFNDVQLPLLADQVRWMHDKYWVDESVVRIIFPEVSAHIEDLRSYENAGENYIDIA
                     WLARKYAYQVTLKDDGLYLTDDTYTSVKRNTNAYYRYDRDSITR"
     gene            complement(19656..20429)
                     /locus_tag="ACGEXS_000890"
     CDS             complement(19656..20429)
                     /locus_tag="ACGEXS_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HFF8011922.1"
                     /translation="MKAHKIFWLNLAAIIIISIVVSGDMFLAMKWEQIHLKDGLKKVL
                     STYPIKNLETLYEIDGHDNPHYENNDQDTWYIESSYSVVGSDELLKEDRMLLKVDKNT
                     HKITGEYDTTTNDRKNATDSTYKSYPVKVVNNKIVFTKDVKDPALKQKIENNQFLIQS
                     GDLTSILNSNDLKVTHDPTTDYYNLSGKLSNDNPNVKQLKRRYNIPKNASTKVELKGM
                     SDLKGNNHQDQKLYFYFSSPGKDQIIYKESLTYNKISEH"
BASE COUNT         7093 a         2964 c         3922 g         6506 t
ORIGIN      
        1 gtaataatcc agcagtagca gctattatca gcacttaaaa gaaaggataa tctttgatgg
       61 tgtaataatt aacttgtgac agaaatatgt aagattatta caatgaacag gctttaactg
      121 attaatattg taatgtgttt tctcttatag taaaatgtaa gcgattacac aactcaacta
      181 tattttccta ataattatat tgttaaggag ggctactttg acaggctttt cagtgtattt
      241 aggacaacct ttagatgaag cgtatattaa gcgaatgatt aaacaaggtt accaaatgat
      301 ttttacatct gtacaaatac cagaagaaga tgacgagaca aaatatcatt atttcacaaa
      361 actactcaat ttattaaaac atgaacaagt gacttacctc atagatgcta atccatctat
      421 tttaacacca tctttttatg accatcttcg acaatatgat gcacaattta tgattcgtat
      481 cgatcatagt acatcaattg aggcaatcga agcgataatg gcacagggtt taaagtgctg
      541 tttgaatgca agtattattt cccgggaatt gttaacaagc ttacatcaac aattgaatga
      601 ttttacatta ctttcatttt gtcataacta ttatccaaga ccagatacgg gattatctgt
      661 tgacttggtc aataagaaaa atgaactcat ttatcaattt aatccaaagg cacaaatata
      721 tggttttatt gtagggagtg atttgcgagg tcctttgcat aaaggcttgc caacaattga
      781 agcaacgaga catagtcatc ctgtcgttgc agctaaatta ttacaagaaa ctggtgtatc
      841 tgaagtgtta gttggagact cattgattga aatgaggcag gcaaaacaac ttatagattt
      901 ttgcaagcat aggcatttca cgttatgtat tgaagaagtg tttgatacga cagtgactta
      961 ccttttcgat atgtgtcata aagtacgccc ggataatccg gaaaatgtca ttcgttcgga
     1021 aacgtcaaga caaatatgtc cacattcgat tcaaccacag tttacgacgc aacgacgcat
     1081 tggttcagta accgttgata atttgaataa cggacgttat caaggcgaaa tgcaaattgt
     1141 gagacaaacg cttagtgcac atgacaatgt gaatgttgtt gcacaaatta ttaaagaaga
     1201 cttaccactg ttaagttgta tcgagccgaa tgatacattt gattttcaaa aaactaggga
     1261 gtgtaagagg tgatggaaaa tagtacgacc gaagcgcgta atgaagcgac gatgcatctt
     1321 gatgaaatga ctgtggaaga ggctttaatt acgatgaata aagaagatca gcaagtcccg
     1381 ttagcagttc gaaaggcaat accacaattg acaaaagtaa ttaaaaaaac aattgcacag
     1441 tataaaaagg gtggacgatt gatttatatc ggtgcaggta caagtggaag gttgggtgtc
     1501 ttagatgcag cggagtgtgt acctacattc aatactgacc ctcatgaaat tataggtatt
     1561 attgctggtg gacaacatgc tatgacgatg gctgtagaag gtgcggaaga tcacaaaaaa
     1621 ttagcggaag aagatttgaa aaatatagat ttaacatcaa aagatgtcgt tataggaatt
     1681 gccgcgagtg gcaaaacgcc atatgttata ggcggtttaa catttgctaa cacaatcggt
     1741 gctacaacag tatctatttc atgcaatgaa catgcagtta taagtgaaat tgcgcagtat
     1801 ccagtagaag ttaaagttgg tccagaagta ttaactggtt caacacgttt aaagtctggt
     1861 acagcacaaa agttaatttt aaatatgatt tcaaccatca caatggttgg tgtcggaaaa
     1921 gtttacgata acctcatgat tgatgttaaa gcaaccaatc aaaaactgat cgaccgttca
     1981 gtgcgtatta ttcaagaaat atgtgctatc acatatgatg aagcaatggc gttatatcag
     2041 gtatctgagc atgatgtgaa agttgcgaca gttatgggta tgtgtggcat ttctaaggaa
     2101 gaagcaacaa gacggttatt aaacaatggt gacattgtta aacgagcaat cagagataga
     2161 caaccttagg agggatttaa atgaccaaag aacaacaact tgcagaacga attattgctg
     2221 cagtaggtgg tatggataat atagatagtg tcatgaactg tatgacacgt gtgcgtatta
     2281 aagtattaga tgagaataaa gtagatgacc aagaactaag gcatattgat ggtgtcatgg
     2341 gtgttataca cgatgaacgc attcaagttg tggttggacc tggtacagtc aataaagtgg
     2401 ctaatcatat ggcggaatta agtggtgtta aactaggtga cccaatacca caccatcaca
     2461 atgatagtga aaaaatggac tataaatcat atgcagctga taaagcaaag gcgaataagg
     2521 aagcgcataa agcaaaacaa aagaatggta agttgaataa agtattgaaa tcaattgcca
     2581 atatctttat accgttgatt cctgcattta ttggagctgg attaattggt ggtattgcag
     2641 cagtactgag taacttaatg gtggcaggct atatttcagg tgcttggatt acgcaactta
     2701 taacagtatt taatgtcatt aaagacggta tgttagcata cttagctatt ttcactggta
     2761 ttaatgcggc taaagaattt ggtgcgacac caggacttgg tggcgtgatt ggtggtacaa
     2821 cgttattaac gggtattgct ggtaaaaata ttttaatgaa tgtcttcact ggagaaccat
     2881 tgcaacctgg acaaggtggg attattggcg ttatttttgc cgtttggatt ttaagtattg
     2941 tcgaaaagag attacataaa attgtgccaa atgcgattga tattattgta acgccgacta
     3001 ttgcattgtt gattgtagga ctattaacta tctttatctt tatgccatta gcaggttttg
     3061 tttcagacag tttagtttca gtagttaacg gaattattag tattggtggc gtatttagtg
     3121 gatttatcat tggtgcaagc ttcctaccgt tagttatgtt agggcttcat catattttta
     3181 cgccaattca tatagaaatg attaaccaat caggtgctac ttacttattg ccaattgcag
     3241 cgatggctgg tgctggacaa gtaggtgccg cattagcact ttgggtaaga tgtaaacgca
     3301 acacaacatt acgtaatact ttaaaaggtg cattgccagt tggtttccta ggtatcggag
     3361 aaccattaat ctatggtgtg actttgccat taggtcgacc tttcttaact gcttgtattg
     3421 gtggtggtat tggtggcgct gtaataggtg gaattggaca tattggtgcc aaagcaatag
     3481 gcccaagtgg tgtgtcacta ttaccattaa tctcagataa tatgtattta ggttatattg
     3541 caggattact tgctgcgtat gctggtggat tcgtttgtac atatttattt ggaacgacaa
     3601 aggcgatgcg acagacagat ttgttgggtg attaatgatg acaaatattt tatatcgcat
     3661 tgataagcag ttgagtgatt ttacgaagac agaaaagata atcgctgatt acattttaaa
     3721 gaatccacat aaaatcattg atatgactgt gaatgatttg gcagatgtta cgaatgttag
     3781 tacagcatca attgttagat ttagtcggaa aatgacacat caaggttttc aagagctaaa
     3841 gattgcgata tctcgatact tacccgaaga tattgcaacc aatccacatt tagaattgat
     3901 tgaaaatgaa tctgtagaaa ctttgaaaaa taaaatgatt gctagagcaa cgaatacgat
     3961 gcgatttgta gctactaata ttatggatgc gcaaattgat gcaatttgtg atgtgttgaa
     4021 aaatgccagg acaatatttt tatttggatt tggcgcatcg agtttgacta ttggtgatct
     4081 ttttcaaaag ttatctcgta ttggcttaaa tgtcaggtta ttacatgaaa cgcatttact
     4141 tgtgtcaaca tttgcgacgc atgatgatag agattgcatg atttttgtga cgaatcaagg
     4201 tagtcatagt gaattgcagt caattgcaca ggtggccaca cattacagta ttcccatcat
     4261 aactatatct agtacagcta ataatccagt ggctcaaatt gcagactatg cattgattta
     4321 tggcagaact gatgaaaatg aaatgcgtat ggcggctaca acgtcactat ttgcacagtt
     4381 attcacggta gatatattgt actatcgatt tgtagcatta aattatcatg cgattctaga
     4441 ttgtataacc caatcgaaaa tggcacttga taattacagg aagcatcttg cgacgataga
     4501 ttttaaacat tagatattgt gtgcgttcta acataaagtt agttgcaacg taaacaattg
     4561 aagtttttga tgtgattcag ttgcttgatg aaaaattgtt gctacaagtt tgtaaaactg
     4621 gcatagaggc tattttgaaa atgataaagg cgtgacaaat taaagcaaag ttcagatttg
     4681 agctttgctt ttttcataga tggaaattaa atgctgaaga ataattttaa tgctaaatga
     4741 actaatacga aggtgctaaa agcaattatt atcaaattag tgatatgaat gaaatgttta
     4801 aatcgtttgc gttgattgta ttcgggactt ttatagagta ggtagtagta cataaagagt
     4861 atggctgcga acatgccgaa tattgaagtg aagttaccta cagtgactgc catgtaatga
     4921 tttaaagtta aatagtaact tagactaata ataaaaaata aaatgatttg ataggtaatt
     4981 aacaccattc aagtccctcc attaatcgta gacaaaaaat ttatcaatga tttgattatt
     5041 tgtattcaaa ttttagtata cgacttacct caaagacaaa tattaaaaga taagacaata
     5101 aaattagaat aaatttacgt ttgaagataa aaaacaaaca tttttcatta aagtttatga
     5161 tatatttaga gcaatagaaa gtgtatggaa ggggatatga atggcatacc aaagtgaata
     5221 cgcattagaa aatgaaatga tgaatcaact tgaacaattg ggttacgaaa gagtaacgat
     5281 acgtgataat aagcaattgc ttgataattt tagaacgatt ttaaatgagc gtcatgcgga
     5341 caaattagaa ggcaatccct taacagataa agaatttcaa cgtctgttaa cgatgattga
     5401 tggaaaaagt attttcgaga gtgcccgtat tttacgtgat aaattaccac ttagacgtga
     5461 tgatgagtct gagatttatt tgtcgttttt agatacgaaa agttggtgta aaaataagtt
     5521 tcaagtgacg aatcaagtat ctgtcgagga tacatataaa gcacgttatg atgtaacgat
     5581 attaatcaac ggactacccc ttgtccaagt tgaattgaaa cgtcgaggta ttgatattaa
     5641 tgaggcgttt aaccaagtaa aacgttaccg caaacaaaat tacacaggct tattccgcta
     5701 catacaaatg tttatcatta gtaatggtgt tgaaacgcga tacttttcta ataatgatag
     5761 cgaactattg aagagtcaca tgttttattg gagtgataaa cagaataacc gtatcaatac
     5821 attgcaatcg tttgctgagt catttatgag accttgtcaa ttagctaaga tgatatcgcg
     5881 ctatatgatt attaatgaaa cagatagaat actgatggca atgcgtccgt atcaagtgta
     5941 tgcggtagaa gcacttattc aacaagcgac tgagacaggg aataatggat atgtatggca
     6001 tacaactgga agtggtaaga cgttgacttc ttttaaagcg agtcagattt tatcacagca
     6061 agatgacatt aagaaagtta tctttttggt tgaccgtaaa gacttggata gtcaaacaga
     6121 agaggaattt aataaatttg ctaagggtgc tgtagacaaa acttttaata cctcgcaact
     6181 ggtacgccaa ctaaatgata aaagtttgcc acttattgta acgacgattc aaaaaatggc
     6241 taaagcgatt caagggaatg cccctttatt agaacagtat aaaacgaata aagttgtatt
     6301 tattattgat gagtgtcatc gcagtcaatt tggtgacatg catcgtctag ttaaacaaca
     6361 tttcaaaaat gcccaatact ttggattcac tggtacgcca cgttttccag aaaatagtag
     6421 tcaagatggt agaacaactg cagatatttt cggtagatgc ttacatacgt atttaattag
     6481 agatgccatt catgatggta atgtacttgg tttctcagtt gactatatta atacttttaa
     6541 aaataaagct ttaaaagcag aagataacag catggttgaa gcaattgata cggaagaagt
     6601 atggttagcg gataaacgtg tggaattagt aacacgacat atcatcaata atcatgataa
     6661 atatacacgt aatcgtcaat attcaagtat atttacagtc caaagtattc acgcgcttat
     6721 taaatattat gagacattta agcgacttaa caaaaagttg gaacaaccgt taacgatagc
     6781 tggtatattt acgtttaaac ctaatgaaga tgatcgtgat ggtgaagtgc catatcattc
     6841 acgtgaaaaa ttagagataa tgattagtga ttataataaa aagttcgaga cgaatttttc
     6901 aacagacaca actaatgagt attttaatca tatttcaaaa aacgttaaaa agggcgttaa
     6961 agatagtaaa attgatatct taatcgttgt taatatgttc ttaactggtt ttgatagtaa
     7021 agtactgaac actttatatg ttgataagaa tttaatgtat catgatttaa ttcaagcgta
     7081 ttcacgtaca aatagggttg aaaaagaatc aaagccattt ggtaaaattg taaactatcg
     7141 tgacttgaaa aaagagacag acgatgcact gagagtattc tcacaaacaa atgatacgga
     7201 tacaatttta atgcgcagtt atgaagagta taaaaaagaa tttatggacg cttatcgtga
     7261 gcttaaaatg attgtgccga caccacacat ggttgatgac attcaagatg aagaagagct
     7321 aaagcgcttt gttgaagctt atcgtttatt agctaaaata atattacgtt taaaagcatt
     7381 tgacgagttt gagtttacaa ttgatgaaat tggaatggat gaacaagaga atgaagacta
     7441 taaaagtaaa tatttagctg tgtacgatca agtaaaaaga gcgacggctg agaaaaataa
     7501 agtatccatt ttaaatgata ttgatttcga aatagaaatg atgcgtaatg atacgattaa
     7561 tgtgaattat attatgaata tattgagaca aattgatctt gaagacaaag cggaacaacg
     7621 tcgtaaccaa gaacaaatta gacgcatttt agatcatgca gatgatccga cattgaggtt
     7681 aaaacgagat ctaattagag aattcatcga caatgttgta ccttctttaa ataaggatga
     7741 tgatatcgat caagaatatg ttaatttcga aagtattaaa aaagaagcgg agttcaaagg
     7801 atttgctgga gagagatcta tcgatgaaca agccctaaaa acaatttcaa atgactacca
     7861 gtatagtggt gttgtaaacc cacatcacct taaaaaaatg attggtgatt tgccattgaa
     7921 agaaaagcgt aaagcaagaa aagccattga atctttcgtg gcagaaacaa ctgaaaaata
     7981 cggtgtgtaa tgattcagcc ccctcgctag attagtgtag ggggcattat tattttctat
     8041 cttttgttag ttactatgtg tttggcaacc atcatttggt ttcaatacaa ttttataatc
     8101 gtatgtgtat agaaataatg tctataggca tttaaaatag tttataatat taacaggata
     8161 agtattaaat gtatgttgaa ggaggtgcgt agctatggat aatagaaata tgattaatcg
     8221 tgtttttagt caaaagatat tacatcaaat tgcaatcaaa aataaaagtg atgttgttga
     8281 tgaggcatat gatttttata tacagggacc taaaaatatc aatgtaatac agaagatgaa
     8341 atctttatat aactatctta aaaagtctta tcgtaacgaa tatttttaca aaaacacaat
     8401 acttaataaa ctccttctag gacgacattc tattaataca actactgcac tttctgagat
     8461 gcccataggg aaaagtattg ctgattttat attgttaaac ggcaaaggcg ttgtctatga
     8521 gattaaaaca gaattagata agctggatag attagataat caaattaatg attattatga
     8581 agtgtttaat tatgtcgtag ttattacaaa tgacaaacat ctgaataaag ttatggctag
     8641 atacaaagat acaacagttg gaattttagt gttaacaact agaaatacac tgagtgaagt
     8701 tcaaaaacct aaagaaaaca atagtctctt aaacacaaaa gcgatgtaca actttttacg
     8761 aaaagaagaa agaaaaagag ttattgcaca aaatcatatg gatgtgccaa cttataatga
     8821 tttcacagag tatgatgtgt tatttgacgt gtttaaagaa ataccaatga cgaaactgca
     8881 taacaatatg atttctgagt tgaaaaaaag aggaaacatg aaagaataca aagatgaatt
     8941 tttagcagcg ccggctgaaa ttaagttctt gttatatttc gcaaaaatga caaagaaaga
     9001 taaaaataaa ctatatcatt ttcttaagga ggattaatat gtattatcct tatttgcgtg
     9061 ggaaacaaaa tgaactattt gcaattagag aattgttaga gaaaggtttg attggtgatt
     9121 gtattcaacc tataattgaa ccaattaaat atacaaccac atttaaaaat actttgcaat
     9181 actgtggtga aaaagcattc tctataaatt tagtagtaaa ttcaaagtta actgaagaag
     9241 agattagtaa cgaaactgtt gcacatttaa ctgaaataat aacaaaaaac aaaagtgtta
     9301 ttcaaaaagc ttacttgggt ccttctgatg aaggcaatga taggttgaaa cagcaatttt
     9361 caagtaatag tttagctatt ttaacaagtg tagatgattg ggaaatgttt ggagataaaa
     9421 ataaacttga aatggttttt gtaccagatg atagacacat taaacgtaaa ttgcgtaata
     9481 ttccaaacaa aggcatcatt atggatcctt ttaataaact aagtcgtaat gttgattatt
     9541 tagataatga tgacgagttt tatagcgacg atcaccttta ttataaggaa gatggatacg
     9601 tagcattttc agactattct gttataggtg gagaatatgt agacggtggc ttttcgccat
     9661 tagctattgc gatacatatt gtctattttg atgaggctaa tgagctaaga gttaagcatt
     9721 ttgtctctga ttctaataat gatagatcaa atccaggtaa aaagtttttt gaggctgtag
     9781 ataaattagt aacatggtca aaaaacttag atattaaaaa tagatcttat gcgcttggac
     9841 aatttgaaga attaaatgaa aataataagt atccaggatt aggtttaatt aaaaggttat
     9901 ctatcatgca tcacctagaa attatgaata gatacttgga gtctcaaaat gaaaatatgt
     9961 gaaaaatgtt ttaataatac tgaaatcgta gaaatcattg caaatgataa tagcaaattt
    10021 gacaattgtg atattgataa caatcatctt ggtgttaaaa tatttgatac gactaaggac
    10081 atagataaat tagaacttat tagagattat ttaagaccag cattagaatt atatgatatt
    10141 agtataaatt taccagatac ttttagccca aaagaaggta aaaagattga aatagcatta
    10201 aaagacgact ggagtatatt taatgttgag gaagctcaaa taagttgtat tttaaatgaa
    10261 ctttttaaag atgatgaaaa tttagataga cgagtgttgg aagatttagt aggcgctaaa
    10321 atcataaacg ataaaaaata tacaaataaa aatctaattg tagcaaataa tgactgggat
    10381 ggattttgtg agagcttgaa gtataaaaat agatttcata ataatatgat caatttagag
    10441 aatttagcgt tctttctcga tatcactact aattactata gtattgagga atttagagaa
    10501 agatttgaac cattatatag atctagaata gttaagttca ttacagagaa aagtgaacta
    10561 atgtcaccac caaagggatt tgcaacggca ggaagattga attctaaatg gataagcgta
    10621 ttatacctta gtacaaaata acaggtcagt atagaagaag ttaaacctaa gcataatgac
    10681 attatttata taggtaaggt taagttacaa aaaaacgtga caaaagagaa attgaaaatt
    10741 gcgaatttaa ctaatttaac tagtaatgca atcaaagctg gcgatgatgg atttagaaag
    10801 tattttgtaa attaccaaac attaaaaaag attcataaag gaataacaaa tccaagcgat
    10861 gaacaaggaa tagattattt gccatttcaa tatttagctg attatattcg aagccttaaa
    10921 tatgacggga taatgtatga gagcatatta caagatggta cttttaactt tgtgtttttt
    10981 gatcaaagtt tatttgaatg tgttaattat gaaagaaaaa gagtaagtga cgttaaatat
    11041 acattgacta agctaagttg acacactcaa aaatctgaat attctaataa aatataaata
    11101 atcccccgca cttcacatag tggcggggga tttattctgg cgttaaaatg cccaaagtat
    11161 gggggtgact cttaaatcat ggcaaagcac ctaaaacttg atatggtgcc gagccctaac
    11221 ttagcacaat atagttagta tgacaaagtc atctaacata acacatcaga agtcgcgcaa
    11281 tttatttcag accatacagt gataaagttg cacaacgcat gacttttatt tagcaataac
    11341 tgctacttct gaaataagtt gctttgcata gtctgactgc ggatgtttga taatatcttc
    11401 tgtgttattc agttcaacga tttcgccatt tttcataact gcaacgcgat cacatatttc
    11461 attgataaca cccatgtcat gtgtgatgaa taaataagtg atgccgaagt ctaactgtaa
    11521 ttgttttaat aactcgatga tatctttttg aattgaaacg tctaaagcgg acactgcctc
    11581 gtcgcaaaca atcactttag gttcaacagc aagtgctctc gcgatactta cacgctgacg
    11641 ttgcccacca gataattcgt gtggatagcg atataagaaa gtttgatcta ggccaacctt
    11701 ttctaacaac gatacgacag ttttaataat gtcatcatta tctttgactt tcccatgaat
    11761 gattagtggt cgtttaatca catcaatgac tttaaatctt ggattaatag atgcgaatgg
    11821 atcttgaaaa atcatttgta tctcttgtcg taaagatttc aattcatcat ctttaaataa
    11881 acttaatggt aattcgttat accaaataaa gccttctgac acttccttta gaccgacgac
    11941 cgtcttagct aatgtcgatt tccctgaccc tgattcaccg acaatgccta atgtttcgcc
    12001 ttttctaata gccaagttaa tatcattaac tgctcggtat aggctgccac tcggtgatgt
    12061 gtaatccacg ctcacgcgat cgaattttaa taaaatatca ttgtttaacg gtcttggcgg
    12121 acgcgtttga tgaatatcag gaatcgcatc tattaagcgt tttgtatagg tatgttgtgg
    12181 cgatttaaaa atactttcaa ccgtgccact ttcaacgaca cttccatctt tcattacaat
    12241 cacatcgtcg caaaattgat acacagcgcc taaatcgtga gtgataaaaa taatagatgt
    12301 ttctgtgtac tcataaaggg acttcattaa ctgcagtaat tgattttgtg tactggcatc
    12361 taatgccgtt gttggttcat ctgcgattaa aatttgtggc tttaaaatca atgccattgc
    12421 tatcatgaca cgttgacgca taccaccaga aagttcatgt ggataagcat caaattgtcg
    12481 agttgcatgt tttataccta ctttttctaa aatgtctatt gtcatcgact ttgcttcaga
    12541 tttagataca cgtttatgtt gaaatattac ttctgtaatt tgtttgccaa tcgttaatct
    12601 tggattcaac gaagagagtg gatcttgaaa aatcattgaa atatccttac ctcgaatttg
    12661 ttgtaacgct gaagttgata aattatttaa cgattgccca ttaaaaataa tttctcctgt
    12721 taatgtgtga tctggataat ctggtagtag ccctaaaata gatttagcgg taatactttt
    12781 tcctgatcct gattcaccaa caatacctag gatatgtttt tttcgtaatt cgaaagagac
    12841 gttttttacc gcttgaactg tagtttcatc ataattgaat tgtacattca gactgttgac
    12901 ttctaataaa tttgacatga tgttgtgcct ccctattcac aaatgtgtaa atttattcta
    12961 tacttttgta tatcatagta atctactcaa gattatttta caaggagtga ttatcataat
    13021 tacttattta gaatctttta agtcgttgta tcacaaggca ttttacaagt ttgtattatc
    13081 ggtattaagc ttgccaatat ttttatatgc agtgataaag tttttctttt ctgctaagag
    13141 gaaaaatttt tacgctaata attctgaaat ttcagaaatt gaacaggcgt tacatcaaaa
    13201 atataaatat ttatcgcagc aaaagtcatc cacacaaata cataaagaag cattaaaaat
    13261 attcaaggca caaagttcta atacgagttc aaagaatatt gaacaagcac atttttcaac
    13321 atactttgaa aatgtattat ttcataagtt catcatgatc aaagtgatat tggccttgcc
    13381 gatgttcatc ttattgactt tttatttaca gccattagtt agatatattt ttgaacgaat
    13441 tgtcatggct gtgattgtca tcattggtgt tattgtcagt gtgtttacca ttctgtattt
    13501 ttcaccgctt gatgcggctt atagcatact gggacaaaat gcaacaaagg cacagataca
    13561 tcaattcaat gtattacatc atcttaacga accttatttt attcaattgt gggataccat
    13621 caagggtgtt tttacctttg acttaggtac gacttacaaa gggaatgagg ttgtgactaa
    13681 agcagttggc gaaagaattc caattacaat aattgtcgca gtattagcgc taatggtggc
    13741 attaattatt gcaataccaa ttggtattat cagtgcgatg aagcgaaata gttggcttga
    13801 tatcacgtta atgataattg cattaattgg tttatctatt ccaagtttct ggcaagggct
    13861 attattcatt ttagcgttct cattgaaatt ggatattttg ccaccatctt atatgccaga
    13921 acatccaata tcgttgattt tacctgtact tgtcattgga acaagtattg ctgcttctat
    13981 cacgcgtatg acaaggtctt ctgtacttga agtaatgcgc agcgattatg ttttaactgc
    14041 ttatgcaaaa ggattatcga cgacacaagt tgttattaaa catattttga aaaatgccat
    14101 tattccaatt gtaacgttag ttggtcttct agtggcagag ttactaggcg gttcagcagt
    14161 gacggaacaa gtatttaaca ttaatggtat cgggcgttat atcgtccaaa aacaactaat
    14221 acctgatatt ccagcagtca tgggtggggt cgtatatata tcaattgtaa tatctttagc
    14281 aaacttaatt attgatatat tttatgcttt aatcgatcca aaattacgta gtgaaatcaa
    14341 cgaaaggaag tgaggcatat ggcacaactt aattcaaaga tagcttcctt aaaattattc
    14401 gcaagttacg ccatagcaac ttatatttta gttatattaa cgagtgcatt aaatcttttt
    14461 aaaggttatg tggccgatac gttctatatt gctgaaacat tgctaatcgt tttaaccatc
    14521 attttaatta ttattttaac aacggaacaa acatggaagc atcatgacct atggcgacgt
    14581 atcgtcgaag tgttgttatt gttgatgaca ttaacaggca acgtatttac attattaatg
    14641 tttgtaagta ttagacgtta ccaacgtaca tcgcaaatac atagttataa cgggtgggaa
    14701 tcgtttatac gaaaaactac tagacatcgt attgcgatta tcgggttact tattttagtc
    14761 tacatgctga cattatcaat tgtgtcacaa tttacatttg atacgacatt ggctactaaa
    14821 aatcagttca atgcactgtt acatggaccg agtctagcct atccgtttgg tactgatgat
    14881 ttcggtagag acttatttac acgcgtagtt gtaggaacga agctgacatt ttcaatttca
    14941 attatttcag tagttattgc agttattttt ggtgtgttac taggcactat cgcaggttat
    15001 tttaatcata ttgataattt aataatgcga attttagatg tagtgtttgc aattccatca
    15061 ttattgttag cggtggcaat tattgcatca tttggagcaa gtattccaaa tttaattatt
    15121 gctttaagta tcggtaatat accatcattt gcacggacaa tgcgtgccag tgttttagaa
    15181 attaaacgca tggaatatgt agatgcagca cgtatcactg gtgaaaacac ttggaatatc
    15241 atatggcgtt atattttacc gaatgcgatt gcgcctatga ttgtacgttt ttcattaaat
    15301 ataggtgtgg ttgtattaac aacaagtagt ttaagtttcc taggacttgg tgttgcacct
    15361 gatgtagctg aatggggcaa cattttacgt accggtagta actacttgga aacgcacagt
    15421 aatttagcta ttgtacctgg tgtttgtatt atgttcgtcg ttttagcatt taattttata
    15481 ggtgatgcag tgcgtgatgc actagatcca agaattcatt aaaaaggtag ggatagatgt
    15541 gaagaaaatc attagtatcg caattatagt tttagcgttg gtattaagtg gttgtggtgt
    15601 ccctacgaaa tcagaagtgg ctcaaaagtc atcgaaagtt gaagtgaaag gcgagcgacc
    15661 aacaatacat ttcctaggac aagcaagtta tgaaaatgat atgaatatcg ttaaagatca
    15721 attggaaaat gcaggattta acgtgaagat gaatatccaa ccagattatg gtagctatcg
    15781 tacacaacgt caagccggca attatgatat ccaaattgat gactggatga cagtgtttgg
    15841 tgacccgaac tatgctatga cggcattatt tagttctaca ggatcaaata gtttattgaa
    15901 agataaacat gtagaccagt tgttaaataa agcttctact caaaatgaag cagatgttaa
    15961 acaaacatat aagcaaattg aagatgaagt tgtatttgat aaagggtata tggcgccttt
    16021 atatggatca aaaaagaatt tagtatatga caataaagtg ttagataaaa atagtgttgg
    16081 attgccaaat tcacgtgcat taatatggca acaatttgat tacaacaata gtagagaacg
    16141 agatacgcgg ccacttgtga tgacacaaca agatggtgaa attcctacat tggatccaat
    16201 acgttcaatt gcgccgtcag tatattcaat taatatgaat atgtacacaa ggttattatt
    16261 attagatgaa aatgatcatt taacaacgaa aggttcgtta agtcatgatt atgctgtgaa
    16321 taaggacaat aaagcatttt atttcttgtt aagagatgat gattattttg cgaaagtggt
    16381 caatggacaa gcacgtaata ctggagagcg tgtatcggct gaagatgtta agttttcttt
    16441 agatagagca cgtgataaaa agtctgtgcc taacaataat acttacaata tgcacaaaca
    16501 tataaatgac atcaagatat taaaagatga ggacatcgat cagttgcgta aagagaaaga
    16561 caaggacgat aaatcaatct atgataagtt gattaaagct tataacgtca aatcgttaac
    16621 gacagatggt caaaaagtaa ataataaaga cggtatttat caaattgtta aaattacgac
    16681 agatcaatcg atgcctcgag aggtaaatta cttaacacac tcttcggcag gcattttatc
    16741 taaaaaattt gttaatcaag taaatcaaga atatccaaaa ggatatgggg atagcagtac
    16801 aattcctgca aattcagatg ggaaaaatgc gctgtatgca agtggcgcat acattatgac
    16861 acagaaaaat gcatatcaag caacgtttca acgtaatcca ggattcaacg aaacagaaaa
    16921 aggtagttat ggaccagcta aaattaaaaa tattacattg aagtttaatg gtgacccgaa
    16981 taatgcattg tcagaactta gaaatcattc aattgatatg ttggcagatg tgaatcaaaa
    17041 acattttgat ttaattaagt cggataaaaa tttaagcatt attcgcaaaa atggacgcaa
    17101 gtcagtcttt ttaatgctaa atattaaaaa aggtatattt aagacgcatc caaacttgag
    17161 acaagcagta gttaatgcga tagatcagga tcaatttatt aagttttatc gtggcgataa
    17221 atttaaaatt gcatcaccga ttacaccact tgtcgatact ggtaacgagc aacgtcaaga
    17281 tttagaaaaa gtagaaaaag ccattaatca ataatgtttt aaatatttaa cagaaagtag
    17341 gaggatatag tatggtcatt aacttaaatg acaaacagac aaaaacatct aaagaagggt
    17401 taatttccgt atcacatcct cttgcggcta aaattggtaa ggatgtatta gatcaaggtg
    17461 gcaacgccat ggatgcagtg attgcaattc aactggcatt gaatgtggta gaaccatttg
    17521 catcaggtat tggtggtggc gggtatttgc tatattatga gcaaagtact ggcagtataa
    17581 ctgcgtttga tgcacgtgag acagcacctg aacatgtaga caaacaattt tatctagatg
    17641 attcaggcga atataaatca ttttttgata tgactacaca tggtaaaact gtcgctgtgc
    17701 cagcaattcc aaagctgttt gattatattc acaagcgtta tgctaaattg tcattggaag
    17761 atttaattaa tcctgcaatt gaactagcca ttgaaggtca tgcagccaat tgggctactg
    17821 aaaaatattc gcgccagcaa cacgcacgat tgacaaagta tcatgaaacg gcacaagtat
    17881 ttacgcatga aaatcaatat tggcgtgaag gtgattggat tgtacaaccc gaattaggta
    17941 agacatttca aatattaaga gaacaagggt ttaatgcatt ttataaaggt gacattgcga
    18001 aacaattagt caatgttgtc aaagcatgtg gtgggacaat cactttagag gatctagcca
    18061 aatatgacat tcagattaaa gcgccaatca gtgcaacatt taaagactat gacatttatt
    18121 caatgggacc atctagttct ggcggtatca cggtaattca aatattgaag ttattagaac
    18181 atgtcgattt accatctatg ggtccaagat ctgttgatta cttgcatcat ttgatacaag
    18241 cgatgcattt agcatatagt gatcgtgcgc aatacttggc ggatgataat tttcatgagg
    18301 tgcctgtaca gtcattaatt gatgatgatt atttaaaagc acgcagtacg ctcattgata
    18361 gcaataaagc aaatattgat atagagcatg gtgttgtgtc tgattgcatt agtcatacag
    18421 atgttgaaga aaatcatacc gaaacaactc atttttgtgt gattgataag gaaggtaata
    18481 ttgcttcatt tacgacatca attggtatga tttatggttc aggtatcacg attccaggct
    18541 acggtgtgtt attgaatacg acaatggatg gctttgatgt agtagatggt ggtattaacg
    18601 aaattgcacc atataaacga ccactaagta acatggctcc aacgattgtg atgtatcacg
    18661 ggaagccaat attaacagta ggtgcacctg gtgccataag tatcattgct agtgttgcgc
    18721 aaacattaat caatgtatta gtgtttggca tggatattca gcaggctata gatgaaccta
    18781 gaatttatag tagccatcct aatcgcattg aatgggagcc tcaattttca caatctacaa
    18841 tattagcatt gattgcacat ggacatgcaa tggaacataa accagatgct tatattggag
    18901 atgtacatgg actacaggtt gacccaacaa cgtatgaagc ttcgggtgga agtgatgata
    18961 ctagagaagg caccgtaatg ggtggcgagg tgttagtgat tagaaaacag ccattaccat
    19021 atcggcaaat gtatgatagt gacggctttc gtctatattt caatgatgtg cagttacctt
    19081 tattggcaga tcaagtgcga tggatgcatg acaaatattg ggttgatgaa agtgttgtta
    19141 gaatcatttt ccctgaagtt agtgcacata ttgaagattt aagaagttat gaaaacgcag
    19201 gagaaaatta tatagatatt gcctggttag cacggaaata tgcttatcaa gtaacattga
    19261 aggatgacgg tttatactta actgatgata catatacttc agtgaaacgg aacacaaatg
    19321 catactatag atatgatcga gatagtatca caagatagtt tatacgcttg atatgaagtt
    19381 tgtagtggat atgagctttt gttgaatgaa aggattcgat ttcgaacttt agcgtgacgc
    19441 ttgagtgtag taatgttgga aatgctatac gaaacgctag tcacctttgt aaaatataca
    19501 acgcctcaca aattcgtttt tgatgtacat cacaatagtt agcctaaaat ctaatgtaat
    19561 gtggtttggt acagttgaac gagttatgtg aggtgttttt ataatatagg attttaatag
    19621 atgatttgga tgtttctata attacagcat aagtattaat gttcacttat tttattataa
    19681 gtaaggcttt ctttataaat gatttggtct tttccaggac ttgaaaaata aaaataaagt
    19741 ttctgatctt gatgattatt gccttttaaa tcactcattc cctttaattc cacctttgtt
    19801 gatgcgtttt taggaatatt atatctacgt tttaattgtt taacgtttgg attatcgttc
    19861 gacaacttac cagataaatt ataataatca gtggtaggat catgtgtgac tttcaaatca
    19921 ttgctattta aaatgcttgt taaatcacca ctttgaatca aaaattgatt gttttcgatt
    19981 ttttgtttca gcgcgggatc ttttacgtct tttgtgaaaa cgattttatt attaactact
    20041 tttactggat aacttttgta tgtcgagtca gtagcatttt ttctatcgtt tgtagttgtg
    20101 tcatattcac cagttatttt atgtgtgttc ttatctacct ttaacaacat acggtcttct
    20161 tttaaaagct catctgatcc aacaactgaa taagaggatt ctatatacca tgtgtcttga
    20221 tcattatttt cataatgggg attatcgtga ccatcaattt cataaagcgt ttctaagttt
    20281 ttaataggat acgtacttag tactttttta agaccatctt tcaaatgaat ttgttcccac
    20341 ttcattgcca aaaacatatc gccactgact acaattgaaa taataataat tgctgctaag
    20401 tttaaccaga aaattttatg tgctttcata cattcccacc gtttctcaaa atacttcatt
    20461 aacactataa taatatattt tgaaa
//
LOCUS       DAXWFK010000031        20332 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.031, whole genome shotgun sequence.
ACCESSION   DAXWFK010000031 DAXWFK010000000
VERSION     DAXWFK010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20332
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.031"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            154..726
                     /gene="pth"
                     /locus_tag="ACGEXS_000891"
     CDS             154..726
                     /gene="pth"
                     /locus_tag="ACGEXS_000891"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFF8011923.1"
                     /translation="MKCIVGLGNIGKRFELTRHNIGFEVVDYILEKNNFSLDKQKFKG
                     AYTIERMNGDKVLFIEPMTMMNLSGEAVAPIMDYYNVNPEDLIVLYDDLDLEQGQVRL
                     RQKGSAGGHNGMKSIIKMLGTDQFKRIRIGVGRPTNGMTVPDYVLQRFSNDEMVTMEK
                     VIEHAARAIEKFVETSRFDHVMNEFNGEVK"
     gene            726..4232
                     /gene="mfd"
                     /locus_tag="ACGEXS_000892"
     CDS             726..4232
                     /gene="mfd"
                     /locus_tag="ACGEXS_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFF8011924.1"
                     /translation="MTILTTLIKEDNHFQDLNQVFGQANTLVTGLSPSAKVTMIAEKY
                     AQSNQQLLLITNNLYQADKLETDLLQFIDAEELYKYPVQDIMTEEFSTQSPQLMSERI
                     RTLTALAQGKKGLFIVPLNGLKKWLTPVEMWQNHQMTLRVGEDIDVDQFLNKLVNMGY
                     KRESVVSHIGEFSLRGGIIDIFPLIGEPIRIELFDTEIDSIRDFDVETQRSKDNVEEV
                     DITTASDYIITEEVISHLKEELKTAYENTRPKIDKSVRNDLKETYESFKLFESTYFDH
                     QILRRLVAFMYETPSTIIEYFQKDAIIAVDEFNRIKETEESLTVESDSFISNIIESGN
                     GFIGQSFIKYDDFETLIEGYPVTYFSLFATTMPIKLNHIIKFSCKPVQQFYGQYDIMR
                     SEFQRYVNQNYHIVVLVETETKVERMQAMLSEMHIPSITKLHRSMSSGQAVIIEGSLS
                     EGFELPDMGLVVITERELFKSKQKKQRKRTKAISNAEKIKSYQDLNVGDYIVHVHHGV
                     GRYLGVETLEVGQTHRDYIKLQYKGTDQLFVPVDQMDQVQKYVASEDKTPKLNKLGGS
                     EWKKTKAKVQQSVEDIAEELIDLYKEREMAEGYQYGEDTAEQTTFELDFPYELTPDQA
                     KSIDEIKDDMQKSRPMDRLLCGDVGYGKTEVAVRAAFKAVMEGKQVAFLVPTTILAQQ
                     HYETLIERMQDFPVEIQLMSRFRTPKEIKQTKEGLKTGFVDIVVGTHKLLSKDIQYKD
                     LGLLIVDEEQRFGVRHKERIKTLKHNVDVLTLTATPIPRTLHMSMLGVRDLSVIETPP
                     ENRFPVQTYVLEQNMSFIKEALERELSRDGQVFYLYNKVQSIYEKREQLQMLMPDANI
                     AVAHGQMTERDLEETMLSFINNEYDILVTTTIIETGVDVPNANTLIIEDADRFGLSQL
                     YQLRGRVGRSSRIGYAYFLHPANKVLTETAEDRLQAIKEFTELGSGFKIAMRDLNIRG
                     AGNLLGKQQHGFIDTVGFDLYSQMLEEAVNEKRGIKEPESEVPEVEVDLNLDAYLPTE
                     YIANEQAKIEIYKKLRKTETFDQIIDIKDELIDRFNDYPVEVARLLDIVEIKVHALHS
                     GITLIKDKGKIIDIHLSVKATENIDGEVLFKATQPLGRTMKVGVQNNAMTITLTKQNQ
                     WLDSLKFLVKCIEESMRISDEA"
     gene            4222..5748
                     /locus_tag="ACGEXS_000893"
     CDS             4222..5748
                     /locus_tag="ACGEXS_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFF8011925.1"
                     /translation="MKHKEAFNGVVVLTAALIVIKILSAVYRIPYQNILGDTGLYAYQ
                     QVYPIVALGMILSMNAIPSAITQNIGKYHSDEAYAKAVAYIQLVGILLFIAIFVFANN
                     IAHMMGDGHLTPMIQAASLSFIFIGMLGVLRGYYQSANNMTVPAISQVIEQVIRVGII
                     IVTIVIFVDRGWTIYEAGTIAILASTIGFLGSSIYLVAHRPFKFKMVNNTAKIVWKQF
                     ALSVLIFAISQLIVILWQVIDSVTIIKSLQAIRVPFDVAITEKGVYDRGASFIQMGLI
                     VTTTFSFALIPLLSDAIKMNNQVLMNRYANASLKITILISTAAGIGLINLLPLMNGVF
                     FKTNDLTLTLSVYMITVICVSLIMMDMALLQAQHAVRPIFVGMTAGLVIKFILNIILI
                     RLSGIIGASISTVVSLIIFGTIIHIAVTRKYHLYAMRRFFINVVLGMVFMSIVVQCVL
                     NIVTTHGRITGLIELLCAAVLGIIALFFYIFRFNVLTYKELTYLPFGSKLYQIKKGRR
                     "
     gene            5748..6941
                     /locus_tag="ACGEXS_000894"
     CDS             5748..6941
                     /locus_tag="ACGEXS_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HFF8011926.1"
                     /translation="MAHTITIVGLGNYGIDDLPLGIYKFLKTQDKVYARTLDHPVIES
                     LQDELTFQSFDHVYEAHNQFEDVYIDIVAQLVEAANEKDIVYAVPGHPRVAETTTVKL
                     LALAKDNTDIDVKVLGGKSFIDDVFEAVNVDPNDGFTLLDATSLQEVTLNVRTHTLIT
                     QVYSAMVAANLKITLMERYPDDYPVQIVTGARSDGADNVVTCPLYELDHDENAFNNLT
                     SVFVPKIITSTYLYHDFDFATEVIDTLVDEDKGCPWDKVQTHETLKRYLLEETFELFE
                     AIDNEDDWHMIEELGDILLQVLLHTSIGKKEGYIDIKEVITSLNAKMIRRHPHIFGDA
                     NAETIDDLKEIWSKAKDAEGKQPRVKFEKVFAEHFLNLYEKTKDKSFDEAALKQWLEK
                     GESNT"
     gene            6938..7201
                     /locus_tag="ACGEXS_000895"
     CDS             6938..7201
                     /locus_tag="ACGEXS_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFF8011927.1"
                     /translation="MRLDKYLKVSRLIKRRTLAKEVSDQGRITINGNVAKAGSDVKVE
                     DVLTIRFGQKLVTVKVTALNEHASKDNAKGMYEIIEERRLEEA"
     gene            7219..7611
                     /gene="divIC"
                     /locus_tag="ACGEXS_000896"
     CDS             7219..7611
                     /gene="divIC"
                     /locus_tag="ACGEXS_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIC"
                     /protein_id="HFF8011928.1"
                     /translation="MKNKVEHIENQYTSQENKKKQRQKMKMRVVRRRITVFAGVLLAI
                     IVVLSILLVVQKHRNDIDAQERKAKEAQFQKQQNEEIALKEKLNNLNDKDYIEKIARD
                     DYYLSNKGEVIFRLPEDKDSSSSKSSKK"
     gene            7716..8117
                     /locus_tag="ACGEXS_000897"
     CDS             7716..8117
                     /locus_tag="ACGEXS_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFF8011929.1"
                     /translation="MSIEVGNKLKGKVTGIKKFGAFVELPEGKSGLVHISEVADNYVE
                     NVEEHLSVGDEVDVKVLSIADDGKISLSIKKAKDRPRRQHTSKPSHQKPVQKAEDFEK
                     KLSNFLKDSEDKLTSIKRQTESRRGGKGSRR"
     gene            8297..9592
                     /gene="tilS"
                     /locus_tag="ACGEXS_000898"
     CDS             8297..9592
                     /gene="tilS"
                     /locus_tag="ACGEXS_000898"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFF8011930.1"
                     /translation="MQLNSNGWHVDDHIVVAVSTGIDSMCLLYQLLNDYKDSYRKLTC
                     LHVNHGVRSASIEEARFLEAYCERHHIDLHIKKLDLSHSLDRNNSIQNEARIKRYEWF
                     DEMMNVLEADVLLTAHHLDDQLETIMYRIFNGKSTRNKLGFDELSKRKGYQIYRPLLA
                     VSKKEIKQFQERYHIPYFEDESNKDNKYVRNDIRNRIIPAIDENNQLKVSHLLKLKQW
                     HDEQYDILQYSAKQFIQEFVKFDEQSKYLEVSRQAFNNLPNSLKMVVLDCLLSKYYEL
                     FNISAKTYEEWFKQFSSKKAQFSINLTDKWIIQIAYGKLIIMAKNNGDTYFRVQTIKK
                     PGNYIFNKYRLEIHSNLPKCLFPLTVRTRQSGDTFKLNGRDGYKKVNRLFIDCKVPQW
                     VRDQMPIVLDKQQRIIAVGDLYQQQTIKKWIIISKNGDE"
     gene            9597..10136
                     /gene="hpt"
                     /locus_tag="ACGEXS_000899"
     CDS             9597..10136
                     /gene="hpt"
                     /locus_tag="ACGEXS_000899"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFF8011931.1"
                     /translation="MHNDLKEVLLTEEDIQNICKELGAQLTKDYQGKPLVCVGILKGS
                     AMFMSDLIKRIDTHLSIDFMDVSSYHGGTESTGEVQIIKDLGSSIENKDVLIIEDILE
                     TGTTLKSITELLQSRKVNSLEIVTLLDKPNRRKADIEAKYVGKKIPDEFVVGYGLDYR
                     ELYRNLPYIGTLKPEVYSN"
     gene            10393..12486
                     /gene="ftsH"
                     /locus_tag="ACGEXS_000900"
     CDS             10393..12486
                     /gene="ftsH"
                     /locus_tag="ACGEXS_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFF8011932.1"
                     /translation="MQKAFRNVLVIVIIGVIIFGLFSYLNGNGNMPKQLTYNQFTEKL
                     EKGDLKTLEIQPQQNVYMVSGKTKNDEDYSSTILYNNEKELQKITDAAKKQNGVKLTI
                     KEEEKQSVFVSILSTLIPVVVIALLFIFFLSQAQGGGSGGRMMNFGKSKAKMYDNNKR
                     RVRFSDVAGADEEKQELIEIVDFLKDNKKFKEMGSRIPKGVLLVGPPGTGKTLLARAV
                     AGEAGAPFFSISGSDFVEMFVGVGASRVRDLFDNAKKNAPCIIFIDEIDAVGRQRGAG
                     VGGGHDEREQTLNQLLVEMDGFGENEGIIMIAATNRPDILDPALLRPGRFDRQIQVGR
                     PDVKGREAILHVHAKNKPLDETVDLKAISQRTPGFSGADLENLLNEASLIAVREGKKK
                     IDMRDIEEATDRVIAGPAKKSRVISKKERNIVAHHEAGHTIIGMVLDEAEVVHKVTIV
                     PRGQAGGYAMMLPKQDRFLMTEQELLDKICGLLGGRVSEDINFNEVSTGASNDFERAT
                     QIARSMVTQYGMSKKLGPLQFGHSNGQVFLGKDMQGEPNYSSQIAYEIDKEVQRIVKE
                     QYERCKQILLEHKEQLILIAETLLTEETLVAEQIQSLFYEGKLPEIDYDAAKVVKDED
                     SEFNDGKFGKSYEEIRKEQLEDGQRDESEDRKEEKDIAEDKKEADKSDEKDEPAHRQA
                     PNIEKPYDPNHPDNK"
     gene            12715..13596
                     /gene="hslO"
                     /locus_tag="ACGEXS_000901"
     CDS             12715..13596
                     /gene="hslO"
                     /locus_tag="ACGEXS_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFF8011933.1"
                     /translation="MTHDYIVKALAFDGEIRAYAALTTETVQEAQTRHYTWPTASAAM
                     GRTMTATAMMGAMLKGDQKLTVTVDGQGPIGRIIADANAKGEVRAYVDHPQTHFPLNE
                     QGKLDVRRAVGTNGSIMVVKDVGMKDYFSGASPIVSGELGEDFTYYYATSEQTPSSVG
                     LGVLVNPDNTIKAAGGFIIQVMPGAKDETISKLEKAISEMTPVSKLIEQGLTPEGLLN
                     EILGEDHVQILEKMPVQFECNCSHEKFLNAIKGLGEAEIQNMIKEDHGAEAVCHFCGN
                     KYKYTEEELNVLLESLA"
     gene            13775..14707
                     /gene="cysK"
                     /locus_tag="ACGEXS_000902"
     CDS             13775..14707
                     /gene="cysK"
                     /locus_tag="ACGEXS_000902"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFF8011934.1"
                     /translation="MAQKPVDNITQIIGGTPVVKLRNVVDDNAADVYVKLEYQNPGGS
                     VKDRIALAMIEKAEREGKIKPGDTIVEPTSGNTGIGLAFVCAAKGYKAVFTMPETMSQ
                     ERRNLLKAYGAELVLTPGSEAMKGAIKKAKELKEEHGYFEPQQFENPANPEVHELTTG
                     PELLQQFEGKTIDAFLAGVGTGGTLSGVGKVLKKEYPNIEIVAIEPEASPVLSGGEPG
                     PHKLQGLGAGFIPGTLNTEIYDSIIKVGNDTAMEMSRRVAKEEGILAGISSGAAIYAA
                     IQKAKELGKGKTVVTVLPSNGERYLSTPLYSFDD"
     gene            14923..15726
                     /gene="folP"
                     /locus_tag="ACGEXS_000903"
     CDS             14923..15726
                     /gene="folP"
                     /locus_tag="ACGEXS_000903"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFF8011935.1"
                     /translation="MTKTKIMGILNVTPDSFSDGGKFNNVESAINRVKAMIDEGADII
                     DVGGVSTRPGHEMVSLEEEMNRVLPVVEAIVGFDVKISVDTFRSEVAEACLKLGVDMI
                     NDQWAGLYDHRMFQIVAKYDAEIILMHNGNGNRDEPVVEEMLTSLLAQAHQAKIAGIP
                     SNKIWLDPGIGFAKTRNEEAEVMARLDELVATEYPVLLATSRKRFTKEMMGYDTTPVE
                     RDEVTAATTAYGIMKGVRAVRVHNVELNAKLAKGIDFLKENENARHNLS"
     gene            15704..16069
                     /gene="folB"
                     /locus_tag="ACGEXS_000904"
     CDS             15704..16069
                     /gene="folB"
                     /locus_tag="ACGEXS_000904"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HFF8011936.1"
                     /translation="MQDTIFLKGMRFYGYHGALSAENEIGQIFKVDVTLKVDLAEAGR
                     TDNVIDTVHYGEVFEEVKSIMEGKAVNLLEHLAERIANRINSQYNRVMETKVRITKEN
                     PPIPGHYDGVGIEIVRENK"
     gene            16066..16542
                     /gene="folK"
                     /locus_tag="ACGEXS_000905"
     CDS             16066..16542
                     /gene="folK"
                     /locus_tag="ACGEXS_000905"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFF8011937.1"
                     /translation="MIQAYLGLGSNIGDRESQLNDAIKILNEYDGINVSNISPIYETA
                     PVGYTEQPNFLNLCVEIQTTLTVLQLLECCLKTEECLHRIRKERWGPRTLDVDILLYG
                     EEMIDLPKLSVPHPRMNERAFVLIPLNDIAANVVEPRSKLKVKDLVFVDDSVKRYK"
     gene            16936..17028
                     /locus_tag="ACGEXS_000906"
     CDS             16936..17028
                     /locus_tag="ACGEXS_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011938.1"
                     /translation="MVNDKVLETSKEMYVEQKCLIFYKTLKENV"
     gene            17081..18568
                     /gene="lysS"
                     /locus_tag="ACGEXS_000907"
     CDS             17081..18568
                     /gene="lysS"
                     /locus_tag="ACGEXS_000907"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFF8011939.1"
                     /translation="MSEEMNDQMLVRRQKLQELYDLGIDPFGSKFDRSGLSSDLKEEW
                     DQYSKEELVEKEADSHVAIAGRLMTKRGKGKAGFAHVQDLAGQIQIYVRKDQVGDDEF
                     DLWKNADLGDIVGVEGVMFKTNTGELSVKAKKFTLLTKSLRPLPDKFHGLQDIEQRYR
                     QRYLDLITNEDSTRTFINRSKIIQEMRNYLNNKGFLEVETPMMHQIAGGAAARPFVTH
                     HNALDATLYMRIAIELHLKRLIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAY
                     ADYHDIMDLTESMVRHIANEVLGSAKVQYNGETIDLESAWTRLHIVDAVKEATGVDFY
                     EVKSDEEAKALAKEHGIEIKDTMKYGHILNEFFEQKVEETLIQPTFIYGHPTEISPLA
                     KKNPEDPRFTDRFELFIVGREHANAFTELNDPIDQKGRFEAQLVEKAQGNDEAHEMDE
                     DYIEALEYGMPPTGGLGIGIDRLVMLLTDSPSIRDVLLFPYMRQK"
     gene            19126..19240
                     /gene="rrf"
                     /locus_tag="ACGEXS_000908"
     rRNA            19126..19240
                     /gene="rrf"
                     /locus_tag="ACGEXS_000908"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            19253..19328
                     /locus_tag="ACGEXS_000909"
     tRNA            19253..19328
                     /locus_tag="ACGEXS_000909"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19286..19288,aa:Val,seq:tac)
     gene            19345..19420
                     /locus_tag="ACGEXS_000910"
     tRNA            19345..19420
                     /locus_tag="ACGEXS_000910"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19378..19380,aa:Thr,seq:tgt)
     gene            19427..19499
                     /locus_tag="ACGEXS_000911"
     tRNA            19427..19499
                     /locus_tag="ACGEXS_000911"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19460..19462,aa:Lys,seq:ttt)
     gene            19533..19607
                     /locus_tag="ACGEXS_000912"
     tRNA            19533..19607
                     /locus_tag="ACGEXS_000912"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19565..19567,aa:Gly,seq:gcc)
     gene            19615..19703
                     /locus_tag="ACGEXS_000913"
     tRNA            19615..19703
                     /locus_tag="ACGEXS_000913"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19649..19651,aa:Leu,seq:taa)
     gene            19709..19785
                     /locus_tag="ACGEXS_000914"
     tRNA            19709..19785
                     /locus_tag="ACGEXS_000914"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19743..19745,aa:Arg,seq:acg)
     gene            19806..19879
                     /locus_tag="ACGEXS_000915"
     tRNA            19806..19879
                     /locus_tag="ACGEXS_000915"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19840..19842,aa:Pro,seq:tgg)
     gene            19903..19978
                     /locus_tag="ACGEXS_000916"
     tRNA            19903..19978
                     /locus_tag="ACGEXS_000916"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19936..19938,aa:Ala,seq:tgc)
     gene            20101..>20332
                     /locus_tag="ACGEXS_000917"
     rRNA            20101..>20332
                     /locus_tag="ACGEXS_000917"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         7187 a         2778 c         4178 g         6189 t
ORIGIN      
        1 ctcaacaatg atacatcata aaaattttaa tgtactcgat tttaaaatac atacttacta
       61 agctaaagaa taatgataat tgatggcaat ggcggaaaat ggatgttgtc attataataa
      121 taaatgaaac aattatgttg gaggtaaaca cgcatgaaat gtattgtagg tctaggtaat
      181 ataggtaaac gttttgaact tacaagacat aatatcggct ttgaagtcgt tgattatatt
      241 ttagagaaaa ataatttttc attagataaa caaaagttta aaggtgcata tacaattgaa
      301 cgaatgaacg gcgataaagt gttatttatc gaaccaatga caatgatgaa tttgtcaggt
      361 gaagcagttg caccgattat ggattattac aatgttaatc cagaagattt aattgtctta
      421 tatgatgatt tagatttaga acaaggacaa gttcgcttaa gacaaaaagg aagtgcgggc
      481 ggtcacaatg gtatgaaatc aattattaaa atgcttggta cagaccaatt taaacgtatt
      541 cgtattggtg tgggaagacc aacgaatggt atgacggtac ctgattatgt tttacaacgc
      601 ttttcaaatg atgaaatggt aacgatggaa aaagttatcg aacacgcagc acgcgcaatt
      661 gaaaagtttg ttgaaacatc acgatttgac catgttatga atgaatttaa tggtgaagtg
      721 aaataatgac aatattgaca acgcttataa aagaagataa tcattttcaa gaccttaatc
      781 aggtatttgg acaagcaaac acactagtaa ctggtctttc cccgtcagct aaagtgacga
      841 tgattgctga aaaatatgca caaagtaatc aacagttatt attaattacc aataatttat
      901 accaagcaga taaattagaa acagatttac ttcaatttat agatgctgaa gaattgtata
      961 agtatcctgt gcaagatatt atgaccgaag agttttcaac acaaagccct caactgatga
     1021 gtgaacgtat tagaacttta actgcgttag ctcaaggtaa gaaagggtta tttatcgttc
     1081 ctttaaatgg tttgaaaaag tggttaactc ctgttgaaat gtggcaaaat caccaaatga
     1141 cattgcgtgt tggtgaggat atcgatgtgg accaatttct taacaaatta gttaatatgg
     1201 ggtacaaacg ggaatccgtg gtatcgcata ttggtgaatt ctcattgcga ggaggtatta
     1261 tcgatatctt tccgctaatt ggggaaccaa tcagaattga gctatttgat accgaaattg
     1321 attctattcg ggattttgat gttgaaacgc agcgttccaa agataatgtt gaagaagtcg
     1381 atatcacaac tgcaagtgat tatatcatta ctgaagaagt gatcagccat cttaaagaag
     1441 agttaaaaac tgcatatgaa aatacaagac ccaaaataga taaatcagtg cgcaatgatt
     1501 tgaaagaaac gtatgaaagc tttaaattat tcgaaagtac atactttgat catcaaatac
     1561 tacgtcgctt agtagcgttt atgtatgaaa caccttcgac aattattgag tatttccaaa
     1621 aagatgcaat cattgcagtt gatgaattta atcgtattaa agaaactgaa gaaagtttaa
     1681 cagtagagtc tgattcgttt attagcaata ttattgaaag tggtaatgga tttataggac
     1741 aaagttttat aaaatatgat gattttgaaa cattgattga aggctatcct gtcacttatt
     1801 tttcattatt cgctacaaca atgccgataa aactaaatca tattattaaa ttttcatgta
     1861 aacctgtcca acaattttat gggcaatatg acattatgcg ttctgaattt caacgatatg
     1921 ttaatcaaaa ctatcatatc gtggttttgg tcgaaaccga aactaaagtt gaacgtatgc
     1981 aagcgatgtt aagtgaaatg catattccat caataacaaa attgcatcgc tcaatgtcat
     2041 cggggcaagc agtgattatt gaaggcagtt tatctgaagg atttgaacta cctgatatgg
     2101 gattagttgt cattactgag cgtgagcttt ttaaatcaaa acagaaaaag caacgaaaac
     2161 gtacgaaagc tatctcaaat gctgaaaaaa ttaagtctta ccaagattta aatgtgggag
     2221 attatattgt tcatgtgcat catggtgttg gtagatattt aggtgttgag acgctcgaag
     2281 tggggcaaac gcatcgtgat tatattaaat tgcaatataa aggtacggat caactatttg
     2341 ttccagtaga tcaaatggat caagttcaaa aatatgtagc ttcggaagat aagacgccaa
     2401 aattaaataa actcggtggc agtgaatgga aaaaaacaaa agctaaagtt caacaaagtg
     2461 ttgaagatat tgctgaagag ttgattgatt tatataaaga aagagaaatg gcagaaggtt
     2521 atcaatatgg ggaagacaca gctgagcaaa caacatttga attagatttt ccatatgaac
     2581 ttacgcctga ccaagctaaa tctatcgatg aaattaaaga tgacatgcaa aaatcgcgtc
     2641 caatggatcg cttgctatgt ggtgatgttg gttatggtaa aactgaagtt gcagtgagag
     2701 cagcattcaa agctgtaatg gaaggaaagc aggttgcatt tttagttcct acaactattt
     2761 tagctcagca acattatgag acgttaattg agcgtatgca agattttcct gttgaaattc
     2821 aattaatgag tcgttttaga acgcctaaag agataaaaca aactaaggaa ggacttaaaa
     2881 ctggatttgt tgacatagtt gttggtacac acaaattact tagtaaagat atacagtata
     2941 aagatttagg gctgttgatt gtagatgaag aacaacgatt tggtgtacgc cataaagagc
     3001 gtattaaaac attaaaacat aatgtagatg tactaacatt gactgcaacc ccaataccta
     3061 gaacattgca tatgagtatg ctaggtgtgc gggatttgtc agtgattgaa acgccgccag
     3121 aaaatcgttt cccagttcaa acatatgtat tagaacagaa catgagtttt atcaaagaag
     3181 ctttagaaag agaactatcc cgtgatggcc aagtgtttta tctttataat aaagtgcaat
     3241 ccatttatga aaaacgagaa caactccaga tgttaatgcc agatgctaac attgcagttg
     3301 ctcatggaca aatgacagag cgcgatttag aagaaacgat gttaagtttt atcaataatg
     3361 aatatgatat tttagtaacg acgacgatta ttgaaacagg tgtcgatgtc ccaaatgcaa
     3421 atactttgat cattgaagat gcagatcgct ttggattgag tcagttgtat caattaagag
     3481 gtcgtgttgg tcgttcaagt cgtattggtt atgcatactt cttacatcca gcaaataagg
     3541 tactaactga gactgcagaa gatcgattac aagcgattaa agaatttacg gagttaggct
     3601 caggatttaa gattgcgatg cgtgatttga acattcgtgg tgctggtaat ttgttaggta
     3661 aacaacagca cggctttatt gatacagttg gatttgattt gtacagtcaa atgttagaag
     3721 aagctgtaaa tgaaaaacgt ggtattaagg aaccagaatc tgaggtgcca gaagtcgaag
     3781 ttgatttaaa cttggatgca tatttgccaa cagaatatat tgcaaatgaa caagctaaaa
     3841 ttgaaattta taaaaagcta cgaaaaactg aaacatttga tcaaattatc gacattaaag
     3901 atgaattaat tgatcgtttc aatgattatc ctgttgaagt agcacgtttg cttgatatag
     3961 tggaaataaa agtacacgca ttacattcag gtatcacgtt gattaaagat aaagggaaaa
     4021 taattgatat tcatttatct gtaaaagcca ctgaaaatat tgatggcgaa gtgctgttca
     4081 aagcaacaca acctttaggt agaacaatga aggttggtgt tcaaaataat gcaatgacaa
     4141 ttactttaac gaaacaaaat caatggcttg atagtttgaa gtttttagtt aagtgcattg
     4201 aagaaagtat gagaatcagt gatgaagcat aaagaagcat ttaatggcgt tgtcgtgtta
     4261 actgctgcat taattgtcat taaaattctg agtgctgtat atcgaattcc atatcaaaat
     4321 atattaggcg atacaggttt gtatgcatat caacaagtgt atccaattgt agcattagga
     4381 atgatattat cgatgaatgc cattcctagt gcaattacac aaaatatagg gaagtatcat
     4441 agtgacgaag catatgcaaa agcagtcgct tatatacaat tagttggtat attattattt
     4501 attgctattt ttgtgtttgc gaacaatatt gcacatatga tgggtgatgg ccatttaaca
     4561 ccaatgattc aagctgcaag tttaagcttt atatttatag gtatgcttgg cgtgttaaga
     4621 ggttattatc aatctgcaaa taatatgaca gttccggcta tttcccaggt tatagaacaa
     4681 gttatacgag taggtattat cattgttact attgttattt ttgtagacag aggttggacg
     4741 atatatgaag cgggaacaat tgctatttta gcatcaacga taggtttttt aggttcttca
     4801 atttatttag tagcgcaccg accttttaag tttaaaatgg taaataacac tgcaaagatc
     4861 gtttggaaac agttcgcact ttcggttttg attttcgcta tcagtcaatt aatcgtaatt
     4921 ttatggcaag tgattgatag tgttactatt attaagtcac ttcaagcgat acgcgtgcca
     4981 ttcgatgttg ccataactga aaaaggagtc tatgaccgtg gtgcatcatt tattcagatg
     5041 ggattgattg taactacaac atttagtttt gcgctcattc ctctgttaag tgacgcaatc
     5101 aaaatgaata atcaggtact tatgaatcgt tatgcaaatg cgtcattaaa gattacgatt
     5161 ttaataagta cagcagcggg aataggatta attaatttat tgcctttaat gaacggtgtg
     5221 ttttttaaga cgaatgattt aaccttaacg ttaagtgttt atatgattac ggtcatttgt
     5281 gtatcgttaa ttatgatgga tatggcatta ttacaagcgc aacatgctgt gagacctatt
     5341 tttgttggta tgacggcagg attggttatt aaatttatac ttaatatcat tttgattcgt
     5401 ttaagtggca ttattggtgc gagcattagt actgttgtat cattaattat attcggtacg
     5461 attatccata ttgctgtcac gagaaaatac cacttatatg cgatgagacg attttttatc
     5521 aatgttgttt taggtatggt atttatgtcg attgttgttc aatgcgtgtt aaacatagtg
     5581 acaacacacg gtagaatcac tggactcatt gaattattat gtgcagcagt attaggtatc
     5641 attgcattgt ttttctatat ttttagattt aatgttttga catataaaga gttaacttat
     5701 ttaccatttg gttcaaagtt gtatcaaatt aagaaaggaa gacgttgatg gcacatacca
     5761 ttacgattgt tggcttagga aactatggca ttgatgattt gccgctaggg atatataaat
     5821 ttttaaagac acaagataaa gtttatgcaa gaacgttaga tcatccagtt atagaatcat
     5881 tgcaagatga attaacattt cagagttttg accatgttta tgaagcacat aaccaatttg
     5941 aagatgtcta tattgatatt gtggcgcaat tggttgaagc tgctaatgaa aaagatattg
     6001 tctatgcggt tccgggtcat cctagagttg ctgagacaac tacagtgaaa ttactggctt
     6061 tagcaaagga caatactgat atagatgtga aagttttagg tgggaaaagc tttattgatg
     6121 atgtgtttga agcagttaat gtagatccaa atgatggctt cacactgtta gatgcgacat
     6181 cattacaaga agtaacactt aatgttagaa cgcatacatt gattacgcaa gtttatagtg
     6241 caatggttgc tgctaatttg aaaatcactt taatggaacg atatcctgat gattaccctg
     6301 ttcaaattgt cactggtgca cgaagcgatg gtgcggataa cgttgtgaca tgcccattat
     6361 atgaattgga tcatgatgaa aatgcattca ataatttgac gagtgtattc gtaccaaaaa
     6421 tcataacatc gacatatttg tatcatgact ttgattttgc aacggaagtg attgatactt
     6481 tagttgatga agataaaggt tgtccatggg ataaagtgca aacgcatgaa acgctaaagc
     6541 gttatttact tgaagaaaca tttgaattgt tcgaagctat tgacaatgaa gatgattggc
     6601 atatgattga agaactagga gatattttat tacaagtgtt attgcatact agtattggta
     6661 aaaaagaagg gtatatcgac attaaagaag tgattacaag tcttaatgct aaaatgattc
     6721 gtagacaccc acacatattt ggtgatgcca atgctgaaac tatcgatgac ttaaaagaaa
     6781 tttggtctaa ggcgaaagat gctgaaggta aacagccaag agttaaattt gaaaaagtat
     6841 ttgcagagca ttttttaaat ttatatgaga agacgaagga taagtcattt gatgaggccg
     6901 cgttaaagca gtggctagaa aaaggggaga gtaatacatg agattagata aatatttaaa
     6961 agtatcacgg ttaataaagc gacgtacgct agcaaaagaa gtaagtgatc aaggtagaat
     7021 tacaataaat ggtaatgttg ctaaagctgg atcggatgtt aaagttgaag atgtgctgac
     7081 gattcgcttt ggtcaaaaat tagtaacagt taaagtaact gcattaaatg aacatgcatc
     7141 taaagataac gcgaagggta tgtatgaaat cattgaagag cgtcgacttg aagaagcgta
     7201 aattggaggt gacaagcaat gaaaaataaa gtagaacata tagaaaatca gtacacgtcg
     7261 caagagaaca agaaaaaaca acgtcaaaaa atgaaaatgc gtgttgttcg taggcgtatt
     7321 acagtatttg cgggcgtatt acttgcgata attgttgttt tatcaatctt gcttgttgtc
     7381 caaaaacatc gcaatgatat tgatgcacag gagcgaaaag cgaaagaagc acagtttcaa
     7441 aagcaacaaa atgaagaaat tgcgttaaaa gaaaagttga ataatctgaa tgacaaagat
     7501 tacattgaaa aaattgcgcg tgatgattat tacttaagca acaaaggtga agtgattttt
     7561 aggttgccag aagacaaaga ttcgtctagc tcaaaatctt cgaaaaaata aatccaaatt
     7621 gattcaaaat tatccgagta tagacattgt gaaaaaatcc aaacaaggat ataataaggg
     7681 aaaatcgaat caaatcggga ggatttattt aacatatgtc aatcgaagtt ggaaataagc
     7741 ttaaaggtaa agtcactggt attaaaaagt ttggtgcatt cgtagaatta cctgaaggaa
     7801 aaagtggttt agttcacatt agtgaagtcg cagataatta tgttgaaaac gtagaagagc
     7861 acctttctgt tggtgatgaa gtagacgtaa aagtattatc tattgctgat gatggaaaaa
     7921 ttagtctttc aattaagaaa gctaaagacc gtccacgtag acaacatacg agtaaaccaa
     7981 gtcatcaaaa accagtgcaa aaagccgaag attttgaaaa gaaattaagc aatttcttaa
     8041 aagatagtga agataaatta acttcaatca aacgtcaaac agaatctaga cgcggtggca
     8101 aaggttcaag acgttaatta aaataaataa agactgtttc gataaggaat atatttagaa
     8161 tgatgcgtat cgaataatcg attgcagcgt tagacaatct aagactgttt cttaaataag
     8221 gagcagtctc ttttatttgt aatgatataa ctaagactta taccattttt gaaaattgta
     8281 aaagtgaggt gatgttatgc agttaaatag taatggttgg catgttgatg accatattgt
     8341 tgtcgctgtt tctacaggta ttgatagtat gtgtttattg tatcaactac taaatgatta
     8401 taaagatagt tatagaaaac taacatgctt acatgtcaat catggcgtta ggtcagcttc
     8461 aattgaggaa gccagatttt tagaagcata ctgcgaacgt catcacatcg atttacatat
     8521 caaaaagtta gatttgtcgc atagtctcga ccgaaataac agcattcaga atgaagctcg
     8581 aattaaacgt tacgaatggt ttgatgaaat gatgaatgta ttagaagcgg atgtattgct
     8641 aacggcgcat catttggacg atcaattaga aactattatg tatcgtattt ttaatgggaa
     8701 atcaacacgt aataaactag gatttgatga gttatcgaag cgaaaaggtt atcagattta
     8761 tcgaccactt ttagctgtct ctaaaaaaga aataaaacaa ttccaagaga gatatcatat
     8821 tccatatttt gaagatgaat ctaataaaga taacaaatat gttagaaatg atattcgtaa
     8881 tagaattatt ccagctattg atgaaaataa tcaacttaaa gtatcgcatt tattaaaatt
     8941 aaaacaatgg catgatgaac aatatgatat tttgcaatat tcagctaaac aatttattca
     9001 agaatttgtg aagtttgatg aacagtcaaa atatttagag gtttctagac aagcttttaa
     9061 taacttacca aactcattaa agatggttgt gttggactgc ctattatcaa agtattatga
     9121 gttgtttaat attagtgcta aaacatacga agagtggttt aaacaattta gtagtaagaa
     9181 agcacaattc agtattaatc tcacggataa atggataatt caaatcgcat atggtaaatt
     9241 aataataatg gctaaaaata atggcgatac atattttaga gttcaaacaa ttaaaaagcc
     9301 aggtaattat atttttaaca aatatcgatt agagatacat tctaatttac caaaatgttt
     9361 atttccgctt acagtgagaa cacgacaaag tggcgataca tttaaactga atgggcgcga
     9421 tggttataag aaagtgaatc gcctgtttat agattgtaaa gtgccacagt gggttcggga
     9481 tcaaatgcca atcgtattgg ataaacaaca gcgcattatt gcggtaggag atttatatca
     9541 acaacaaaca ataaaaaaat ggattataat tagtaaaaat ggagatgaat agcgttatgc
     9601 ataatgattt gaaagaagta ttgttaactg aagaagatat tcaaaatatc tgtaaggaat
     9661 tgggagcaca attaacaaag gattatcaag gtaaaccatt agtatgcgtg ggtatcttaa
     9721 aaggctcagc aatgtttatg tcagatttaa ttaaacgaat tgatacccat ttatcaattg
     9781 atttcatgga tgtttctagt tatcacggag gcactgagtc aactggtgaa gttcaaatca
     9841 ttaaagattt aggttcttct attgaaaata aagacgtatt aattattgaa gatatcttag
     9901 agactggtac tacacttaag tcaattactg aattattaca atctagaaaa gttaattcat
     9961 tagaaatagt tactttatta gataaaccaa accgtcgtaa agcggacatt gaagctaagt
    10021 atgtaggtaa aaaaatacca gatgaatttg ttgttggtta cggtttagat tatcgtgaat
    10081 tataccgaaa cttaccatat atcggtacgt taaaacctga agtgtattca aattaatttt
    10141 ttaatcaatt tcagttatta ttactatgcg tttgagaaat aatagtgtag actcaaaaat
    10201 atgaaaaatg tatttcatat atatttaatt ttagacaaga catatgtctt gaaaagttga
    10261 aaaatataga gattgataaa actaatacgg gtgtgaatga cattgatgtt aagctcaatt
    10321 actagcttat aaaacatgtc atatgttaca atttttgtta gttttattat gggaagtagg
    10381 aggaaatgac gcatgcagaa agcttttcgc aatgtgctag ttatcgtaat aataggcgtt
    10441 attatttttg gtctattttc atatttaaac ggtaatggaa atatgccgaa acagcttaca
    10501 tataatcaat ttactgagaa gttggaaaaa ggtgacctta aaactttaga aatccaacca
    10561 caacaaaatg tctatatggt aagtggtaaa acgaaaaatg atgaagacta ttcatcaact
    10621 attttatata acaacgaaaa agaattacaa aaaattactg atgctgctaa aaagcaaaac
    10681 ggtgtaaaat taacgattaa agaagaagaa aaacaaagtg tctttgtgag tatactttca
    10741 acattaattc cagttgtagt catagcgtta ttatttattt tcttcctaag ccaagcacaa
    10801 ggtggcggta gtggcggtcg tatgatgaac tttggtaaat ctaaagcaaa aatgtacgat
    10861 aataataaac gtcgtgttcg tttctctgat gtagcagggg cagatgaaga aaaacaagaa
    10921 ttaattgaaa ttgttgattt cttgaaagat aataaaaaat tcaaagaaat gggatctagg
    10981 attcctaaag gtgtcttact tgttggacct ccaggtactg gtaaaacatt acttgctaga
    11041 gcggttgcag gtgaagctgg cgcaccattc ttctctatta gtggttcaga ctttgtagag
    11101 atgtttgttg gtgttggtgc gagccgtgtt cgtgacttat tcgataatgc taagaaaaac
    11161 gcgccttgta tcatctttat cgatgagatt gatgctgttg gtcgtcaacg tggtgcaggt
    11221 gttggtggcg gtcatgatga acgtgaacaa accctaaacc aattattagt tgaaatggat
    11281 ggtttcggtg aaaatgaagg tatcattatg atagctgcta caaaccgtcc tgatatcctt
    11341 gacccagcct tattacgtcc aggtcgtttt gatagacaaa ttcaagttgg tcgtccagat
    11401 gtgaaaggcc gtgaagcaat tcttcatgtt catgctaaaa acaaaccact tgatgaaacg
    11461 gttgatttaa aagcaatttc acaacgtaca cctggtttct caggtgctga tttagagaac
    11521 ttattaaatg aagcatcttt aattgctgta cgtgaaggta aaaagaaaat tgacatgaga
    11581 gatatcgaag aggcaacgga tagagttata gccggacctg ctaagaaatc tcgagttatt
    11641 tctaagaaag aacgtaatat tgttgctcat cacgaagctg gtcatacaat tatcggtatg
    11701 gtacttgatg aggcagaagt agtgcataaa gttactattg ttccacgtgg acaagcaggt
    11761 ggttatgcaa tgatgctacc taaacaagat cgtttcttaa tgactgaaca agagttatta
    11821 gataaaatct gtggtttact tggtggacgt gtatcagaag atattaactt taacgaagta
    11881 tcaacaggtg cttcaaatga cttcgaacgt gcaacacaaa tcgcacgctc aatggttacg
    11941 caatatggta tgagtaaaaa attaggacca ttacagttcg gtcatagcaa tggtcaagta
    12001 ttcttaggta aagatatgca aggtgagcct aattattcaa gccaaattgc atatgaaatt
    12061 gataaagaag ttcaacgaat cgttaaagaa caatacgaac gttgtaaaca aattttatta
    12121 gagcacaaag aacaattaat tttaattgct gaaacattat taacagaaga aacattagtt
    12181 gctgaacaaa ttcaatcatt attctacgaa ggtaaattac ctgaaattga ttatgatgca
    12241 gctaaagttg ttaaagatga agattctgaa tttaatgatg gtaaattcgg taaatcttat
    12301 gaagagattc gtaaagagca attagaagat ggacaacgtg acgaaagtga agatcgtaaa
    12361 gaagaaaaag atattgctga ggataaaaaa gaagctgata aatctgatga aaaagatgaa
    12421 ccagcacatc gacaagcccc aaatatcgaa aaaccttacg atccaaatca cccagacaat
    12481 aaataatcga ttatattcag tacctctttc tatgataaag ttatagaaag aggtactttt
    12541 atcgtttttg aaaatacgta ttagatttta agtcgttgaa ttgttatagc agaaaataat
    12601 tgtaaaacaa gttacttcat tatttagaat gatgggtgta gaataagtac aattgttgca
    12661 ttttatgaag taaagtaatt ttttaaatat agagtaatag aggagattga aataatgaca
    12721 cacgattata ttgttaaagc attagcattt gatggagaga ttagggctta tgctgctttg
    12781 acaactgaaa ctgttcaaga agcacaaacg agacattata catggccgac agcatctgct
    12841 gcaatgggaa gaacaatgac agcaacagct atgatgggcg caatgttgaa aggtgatcaa
    12901 aaattaactg tcactgtaga tggccaagga cctattggac gaattattgc cgatgcaaat
    12961 gctaaaggcg aggtgcgtgc ttatgtagac catccacaaa ctcattttcc attaaatgag
    13021 caaggtaaac ttgatgtaag acgagcggta gggacaaatg gatctattat ggttgttaaa
    13081 gacgttggaa tgaaagacta tttctctgga gcaagtccaa ttgtttcagg agaacttggt
    13141 gaagatttta cttattatta tgctacaagt gaacaaacac cttcatcggt aggtcttggt
    13201 gtattggtaa atcctgataa tacgattaaa gcagcaggag gatttatcat tcaagttatg
    13261 ccaggtgcca aagatgaaac aatttcaaaa ttagaaaaag caattagtga aatgacacca
    13321 gtttctaaat taattgaaca aggattaacg ccagaaggat tactaaacga aatcttaggt
    13381 gaagaccatg tgcaaatttt agagaaaatg cctgttcaat ttgaatgtaa ttgtagtcat
    13441 gagaaatttt taaatgctat taaaggattg ggcgaggctg agattcaaaa tatgattaaa
    13501 gaagatcatg gtgctgaagc agtatgtcat ttctgtggaa ataaatataa atatactgaa
    13561 gaagaattaa acgtgttgct agaaagttta gcgtaattta atttaaatca atacgctaaa
    13621 atgtttattt ttagcggttt agtgaaatgt agaactaaat agttgtataa tccttagtga
    13681 ttttgtttgc tttctagaat ttatttgata aaataattct atatccgata aataaactaa
    13741 gatttcaaca actaactaaa aaggagtgtt cttaatggca caaaaaccag tagataatat
    13801 tactcaaatt attggcggta caccggtagt caaattgaga aatgtagtag atgacaatgc
    13861 agcagatgtt tatgtaaaat tggaatatca aaatccaggt ggttctgtaa aggatagaat
    13921 tgctttagca atgattgaaa aagcagagcg agaaggcaaa attaaacctg gcgatacaat
    13981 tgtagaacca acaagtggta atacaggtat cggtttagca tttgtatgtg ctgctaaagg
    14041 atataaagca gtatttacta tgcccgaaac aatgagccaa gagcgtcgta atttattaaa
    14101 agcatacggt gcggaattag ttttaacgcc tggatcagaa gcgatgaaag gtgcaattaa
    14161 aaaagctaaa gaattgaaag aagaacatgg ttacttcgag ccacaacaat ttgaaaaccc
    14221 tgcgaaccct gaagttcatg agttaactac aggtcctgag ttattacaac aatttgaagg
    14281 gaaaactatc gatgcgttcc tagctggtgt tggtactggt ggtacgttat ctggtgtagg
    14341 taaagttctg aaaaaagaat atcctaacat cgaaattgtt gctatagagc ctgaggcttc
    14401 tccagtattg agcggtggtg agccaggtcc acataaatta caaggtttag gtgctggatt
    14461 tattccaggc actttgaata cagaaatcta tgacagtatt attaaagtag gaaatgatac
    14521 agcgatggaa atgtctcgtc gagttgctaa agaggaaggt attttagcag gtatttcatc
    14581 aggtgctgcg atttatgctg ccattcaaaa agcaaaagaa ttaggaaaag gtaaaacagt
    14641 agtaacagta ttgccgagta atggtgaacg ctacttatca acacctttat attcattcga
    14701 tgactaatta atgtcattta aaagagtgag ttatcttttt gagataactt gctctttttt
    14761 tctaccatgt atatttttaa aaatatgagc gttaaattaa acatttttct gataaaaata
    14821 tccagtgaat gataagataa taaacgtaca tactaataac tagtaaatag caggagtaaa
    14881 ttttattaga gttaaacaat acataattaa agggtggtta acatgactaa aacaaaaatt
    14941 atgggcatat taaacgtcac acctgattca ttctcagatg gtggaaaatt taataatgtt
    15001 gaatcagcta taaatagagt gaaagccatg atagatgaag gtgctgacat tatagatgtt
    15061 ggaggtgttt caacgagacc cggtcatgaa atggtttcat tagaagaaga gatgaacaga
    15121 gtattacctg ttgttgaagc tattgtcggt tttgatgtaa aaatttcagt cgatacattt
    15181 cgaagtgagg ttgctgaagc atgtttaaaa ttaggcgttg atatgattaa tgatcaatgg
    15241 gcgggtctgt atgatcatcg tatgttccaa attgtagcta aatatgacgc ggaaattatt
    15301 ttaatgcata atggaaatgg taatcgtgat gaaccggttg tcgaagaaat gttaacatct
    15361 ttgttagcac aagcacatca agctaaaata gctggtatac cttcaaataa aatttggcta
    15421 gatccaggta taggtttcgc taaaactaga aatgaagaag ccgaagttat ggcaagactg
    15481 gatgaacttg ttgcaacaga atatccagtt ttattagcga caagccggaa acgtttcact
    15541 aaagagatga tgggttatga tacaacaccg gttgaaagag atgaagtaac tgcagctacg
    15601 actgcatatg gtattatgaa aggcgttaga gcagtacgcg ttcataatgt cgagttgaat
    15661 gctaaattag ctaaaggtat agatttttta aaggagaatg aaaatgcaag acacaatctt
    15721 tcttaaaggt atgcgctttt atggatatca tggtgcttta tcagctgaaa atgaaatagg
    15781 gcaaattttc aaagtggatg taactttgaa agtagactta gctgaagctg ggcgtactga
    15841 taatgttatt gatacagttc attatggtga agtgttcgaa gaggttaaat caattatgga
    15901 aggtaaggcc gttaatttac ttgagcatct agctgaacgt attgcaaatc gtataaattc
    15961 acaatataat cgtgtaatgg aaacgaaagt gagaatcact aaagaaaacc caccgattcc
    16021 gggtcattat gatggagtag gtatcgaaat agtgagggag aataaatgat tcaagcatac
    16081 ttaggattag gtagtaatat tggtgataga gaaagccagt taaacgatgc tataaagatt
    16141 ttgaatgaat atgatggtat taacgtatct aatatttctc cgatttatga aacagcacca
    16201 gttgggtata ctgagcaacc taacttttta aatttgtgtg ttgaaattca aacaacactc
    16261 acagtattac aactgttgga atgttgtttg aagacagaag aatgtttaca ccgtattaga
    16321 aaggaacgat ggggtcctag aactttagat gtggatattt tgttgtatgg agaagaaatg
    16381 atagatttac caaaactgtc ggtgccacat ccgagaatga atgaacgtgc atttgtttta
    16441 atcccattaa atgatatagc agcaaatgtc gtagaaccac gttcgaaatt gaaagtgaaa
    16501 gatttagttt ttgtcgatga cagtgtaaag agatataaat aatgtattgt tgagaacatt
    16561 catatttatt gggaatagat tattgattat tggagatgtt tccgcatttt gattatctct
    16621 aaatgcattt gatttcgaat tagtatatga tgatttagta tgtgttagaa ttaagttatt
    16681 tcataaaatc aaagtaagca atatttgata aattgctgta atttatttag gcttttgttg
    16741 aagtttgtgg aagataattt ttatacattt acttagggtt gtattaatga taatcgtatt
    16801 ttaaatgaat aagtaattca taaacgaaaa cagaaattgc tttattacat ttacaagctc
    16861 ttgtaaaatt aaaaaagtga taaaattgat gttatgaata taagaaaaca tgccacatca
    16921 taactttaag gtgtaatggt taatgataaa gtattagaaa catcgaaaga gatgtatgtt
    16981 gagcaaaaat gtctgatatt ttataaaact ttaaaggaaa atgtttgagt gtaccagttg
    17041 gaatactaaa ggattacaac aagttaaagg agagaaagtt atgtcagaag aaatgaatga
    17101 ccaaatgttg gttcgacgtc aaaaattaca agaattatat gatcttggta tagacccgtt
    17161 tggttctaaa tttgaccgtt caggtttatc tagtgatttg aaagaagagt gggaccagta
    17221 ttctaaagaa gaattggtag aaaaagaagc ggatagtcat gtcgctatag ctggacgatt
    17281 aatgactaag cgtggtaaag gtaaagcagg atttgcacac gttcaggact tagctggaca
    17341 aattcaaatt tacgttcgta aagatcaagt tggcgatgac gaatttgatt tatggaaaaa
    17401 tgctgattta ggcgatatcg ttggtgttga aggtgtaatg ttcaaaacaa atactggcga
    17461 attatcggtt aaagcgaaga aattcacgct actaactaaa tcattgcgac cattaccgga
    17521 taaattccac ggtttacagg atattgaaca gagatatcgt caaagatatt tagatttaat
    17581 tacgaacgaa gatagcactc gtacatttat taatcgtagt aaaatcattc aagaaatgcg
    17641 taattattta aataataaag gtttcttgga agtagaaaca cctatgatgc accaaattgc
    17701 tggtggagca gctgctagac catttgtaac acatcataat gcattagatg caacgttata
    17761 catgcgtatt gctattgagt tgcatttaaa acgtttaatt gtcggtggac ttgaaaaagt
    17821 atatgaaatt ggtagagtat tccgtaatga aggtgtatca actagacata accctgaatt
    17881 cacaatgatt gaattatatg aagcatatgc agattatcat gacattatgg atttaacaga
    17941 atctatggtg agacatattg ccaatgaagt gttaggttct gcaaaagtac aatacaatgg
    18001 ggaaacgatt gatttagaat ctgcttggac tcgtttgcat attgttgatg ctgtaaaaga
    18061 agctactggt gtagattttt atgaagttaa aagtgatgaa gaagctaaag ctttagctaa
    18121 agaacatggt attgaaatta aagatacaat gaaatatggt catattttaa atgaattctt
    18181 tgagcaaaaa gttgaagaaa cacttattca gccaacgttt atctatggtc atccgactga
    18241 aatttcacct ttagcgaaga aaaatcctga agatcctaga tttactgatc gtttcgaatt
    18301 gttcattgta ggtagagagc atgcaaatgc atttactgaa ttaaatgatc ctattgatca
    18361 aaaaggtcgt tttgaagcgc aacttgttga aaaagcgcaa ggtaatgatg aagcgcatga
    18421 aatggatgaa gattacattg aagcgttaga atatggtatg cctccgacag gtggtcttgg
    18481 tatcggtatt gacagattgg ttatgttatt aactgactct ccatcaatca gagacgtatt
    18541 attattccct tatatgagac aaaaataaat gacgttgatt gttagtaaga gctctcgtgt
    18601 atacaacatg tgtatgcgag ggctttctta attatggtaa ttagttcgtg tttgaatgtt
    18661 tttgatagta atagttaacg atagtggtgc tatttttgac tgttaaacaa ggtagttggc
    18721 tgatagatga aaatgacgat acgtatatag agtagttcgg atgagaaatg ttaacgatag
    18781 acattgagat atcttataac aaaaattgct atattagtat aatttatctt aatcagctat
    18841 aaaagtactt taaaattgta tagaatgtgt atggttttgt acatatgtgt atgatagaat
    18901 actaaaagtg ttatgaatta gagggcacga gaaatgtcag ttttgaagaa taaaaaagtt
    18961 gattaaaagt gttgacttta tcaattgaat gaagtaatat ataaaagtcg tcaaaaacag
    19021 acgaaacaca ctaaaagctg atgtgacaaa gtttacatca aagtgtaaaa ttaactattg
    19081 caccttatta attaagcgtg tatcatgaat aagtaagtta ttttgtctgg tgactatagc
    19141 aaggaggtca cacctgttcc catgccgaac acagaagtta agctccttag cgtcgatggt
    19201 agtcgaactt acgttccgct agagtagaac gttgccaggc aaatgacaaa tcggagaatt
    19261 agctcagctg ggagagcatc tgccttacaa gcagagggtc ggcggttcga acccgtcatt
    19321 ctccaccatt tattcttaga tatagccggc ctagctcaat tggtagagca actgacttgt
    19381 aatcagtagg ttgggggttc aagtcctctg gccggcacca tcttttgagc cattagctca
    19441 gctggtagag catctgactt ttaatcagag ggtcagaggt tcgaatcctc tatggctcat
    19501 tacgatttaa tttttatatt tagcaaaata atgcagaagt agttcagcgg tagaatacaa
    19561 ccttgccaag gttggggtcg cgggttcgaa tcccgtcttc tgctccatta ttttgccggg
    19621 gtggcggaac tggcagacgc acaggactta aaatcctgcg gtgagagatc accgtaccgg
    19681 ttcgattccg gtcctcggca ccattttagc gcccgtagct caattggata gagcgtttga
    19741 ctacggatca agaggttatg ggttcgactc ctatcgggcg cgccattttt aaattaattg
    19801 aataacggga agtagctcag cttggtagag cacttggttt gggaccaagg ggtcgcaggt
    19861 tcgaatcctg tcttcccgat tacttcttaa attccatttt atgggggctt agctcagctg
    19921 ggagagcgcc tgctttgcac gcaggaggtc agcggttcga tcccgctagt ctccaccatt
    19981 tattttttac acgatgaaca ttgaaaactg aatgacaata tgtcaacgtt aattccaaaa
    20041 aacgtaacta taagttacaa acattattta gtatttatga gctaatcaaa catcataatt
    20101 tttatggaga gtttgatcct ggctcaggat gaacgctggc ggcgtgccta atacatgcaa
    20161 gtcgagcgaa cggacgagaa gcttgcttct ctgatgttag cggcggacgg gtgagtaaca
    20221 cgtggataac ctacccataa gactgggata acttcgggaa accggagcta ataccggata
    20281 atattttgaa ccgcatggtt caaaagtgaa agacggtctt gctgtcactt at
//
LOCUS       DAXWFK010000032        20264 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.032, whole genome shotgun sequence.
ACCESSION   DAXWFK010000032 DAXWFK010000000
VERSION     DAXWFK010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20264
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.032"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(302..2710)
                     /gene="priA"
                     /locus_tag="ACGEXS_000918"
     CDS             complement(302..2710)
                     /gene="priA"
                     /locus_tag="ACGEXS_000918"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFF8011940.1"
                     /translation="MIAKVIVDVASKSVDYKFDYIIPEQLESVIQPGVRVIVPFGPRT
                     IQGYVMEVTAEPDAQLDVSKLKKIIEVKDIQPELTSELIALSEWMGSTHVIKRISMLE
                     VMLPSAIKAKYKKAFKMKDDIELPSALLQKFDKHGYYYYKDAQKNNDIQLLMKLLKDD
                     IVEEKTILTQNITKKTKRAVRVIEGYHPDEVLAKLEKVIKQYDLYAYLSEEQHKTIFL
                     TDIEDMGFSKSSLDGLIKKGYVEKYDAVVERDPFKDRVFEQESKQQLTEDQYKAYEAI
                     KAKIVSQEQETFLLHGVTGSGKTEVYLQTIEDVLSQGKQAMMLVPEIALTPQMVLRFK
                     RRFGDDVAVLHSGLSNGERYDEWQKIRDGRARVSVGARSSVFAPFKNLGLIIIDEEHE
                     STYKQEDYPRYHAREIAQWRSEYHHCPVILGSATPCLESYARAEKGVYHLLSLPNRVN
                     QQALPEIDIVDMREELSEGNRSMFSKDLREAIQLRLDRQEQVVLFLNRRGYASFMLCR
                     DCGYVPQCPNCDISLTYHKTTDLLKCHYCGYQETPPNQCPNCESEHIRQVGTGTQKVE
                     ELLQQEFEDARIIRMDVDTTSKKGAHEKLLTEFEKGNGDILLGTQMIAKGLDYPNITL
                     VGVLNADTMLNLPDFRASERTYQLLTQVAGRAGRHEKAGQVIIQTYNPDHYSILDVQK
                     NDYLTFYRQEMEYRKLGKYPPYYYLINFTISHKEMKKVMVASQHVHKILLQHLTEKAL
                     VLGPSPAALARINNEFRFQILVKYKSEPGLLQAIQFLDDYYHEKFIKEKLALKIDIDP
                     QMMM"
     gene            complement(2710..3909)
                     /gene="coaBC"
                     /locus_tag="ACGEXS_000919"
     CDS             complement(2710..3909)
                     /gene="coaBC"
                     /locus_tag="ACGEXS_000919"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFF8011941.1"
                     /translation="MKKILLAVTGGIAAYKAIDLTSKLTQSGYEVRVMLTNHAQKFVT
                     PLAFQAISRNAVYTDTFIEENPSEIQHIALGDWADAIIVAPATANTIAKLSVGIADDL
                     VTSTLLATETPKFIAPAMNVHMYENKRTQQNINILKEDGYHFIEPGSGFLACGYVAKG
                     RMEEPLQIVSVIDAHFQNSNRLANSSFQDKRALVTAGPTIEVIDPVRFVSNRSSGKMG
                     YAIAEALRNRGAIVTLVAGPTTLEDPKDIEVIHVQSAEEMFEQVTSRFDEQDIVVKAA
                     AVSDYTPVDVLEHKMKKQDGDLSVSFKRTKDILKYLGEHKTSQYLIGFAAETEDIENY
                     AQQKLRKKNADVIISNNVGDMSIGFSSDDNELTMHFKNNEKVNIKKGKKVVLAAQILD
                     ELETRWQ"
     gene            complement(4125..4343)
                     /gene="rpoZ"
                     /locus_tag="ACGEXS_000920"
     CDS             complement(4125..4343)
                     /gene="rpoZ"
                     /locus_tag="ACGEXS_000920"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFF8011942.1"
                     /translation="MLNPPLNQLTSQIKSKYLIATTAAKRAREIDEQPETELLSEYHS
                     FKPVGRALEEIADGKIRPVISSDYYGKE"
     gene            complement(4343..4966)
                     /gene="gmk"
                     /locus_tag="ACGEXS_000921"
     CDS             complement(4343..4966)
                     /gene="gmk"
                     /locus_tag="ACGEXS_000921"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFF8011943.1"
                     /translation="MDNEKGLLIVLSGPSGVGKGTVRKRIFEDPSTSYKYSISMTTRQ
                     MREGEVDGVDYFFKTRDAFEALIKDDQFIEYAEYVGNYYGTPVQYVKDTMDEGHDVFL
                     EIEVEGAKQVRKKFPDALFIFLAPPSLEHLRERLVGRGTESDEKIQSRINEARKEVEM
                     MNLYDYVVVNDEVELAKNRIQCIVEAEHLKRERVEAKYRKMILEAKK"
     gene            5241..6938
                     /locus_tag="ACGEXS_000922"
     CDS             5241..6938
                     /locus_tag="ACGEXS_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HFF8011944.1"
                     /translation="MAYDGLFTKKMVESLQFLTTGRVHKINQPDNDTILMVVRQNRQN
                     HQLLLSIHPNFSRLQLTTKKYDNPFNPPMFARVFRKHLEGGIIESIKQIGNDRRIEID
                     IKSKDEIGDTIYRTVILEIMGKHSNLILVDENRKIIEGFKHLTPNTNHYRTVMPGFNY
                     EAPPTQHKINPYDITGAEVLKYIDFNAGNIAKQLLNQFEGFSPLITNEIVSRRQFMTS
                     STLPEAFDEVMAETKLPPTPIFHKNHETGKEDFYFIKLNQFNDDTVTYDSLNDLLDRF
                     YDARGERERVKQRANDLVRFVQQQLHKYQNKLAKLIEEYEQSKNKDTEQLYGELITAN
                     IYRIKQGDKEVTALNYYTNEEVVIPLNPTKSPSANAQYYYKQYNRMKTRERELQHQIQ
                     LTKDNIDYFSTIEQQLHHISVHDIDEIRDELAEQGFMKQRKNQTKKKKAQIQLQHYVS
                     TDGDDIYVGKNNKQNDYLTNKKAKKTHTWLHTKDIPGSHVVIFNDAPSDTTIKEAAML
                     AGYFSKAGNSGQIPVDYTLIKNVHKPSGAKPGFVTYDNQKTLYATPDYELIQKMKQS"
     gene            complement(7201..7602)
                     /locus_tag="ACGEXS_000923"
     CDS             complement(7201..7602)
                     /locus_tag="ACGEXS_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFF8011945.1"
                     /translation="MDIPKITTFLMFNNQAEEAVKLYTSLFEDSEIITMAKYGENGPG
                     DPGTVQHSIFTLNGQVFMAIDANSGTELPMNPAISLFVTVKDTIEMERLFNGLKDEGA
                     ILMPKTNMPPYREFAWVQDKFGVSFQLALPE"
     gene            complement(8039..8251)
                     /locus_tag="ACGEXS_000924"
     CDS             complement(8039..8251)
                     /locus_tag="ACGEXS_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011946.1"
                     /translation="MNDKTSNDLYGKIKHCNEFINHSNDSNLSSSHDVDESSTKQKHI
                     KNKTTIDHNDDLFKHVKDILRKQGQI"
     gene            complement(8281..8892)
                     /gene="pyrE"
                     /locus_tag="ACGEXS_000925"
     CDS             complement(8281..8892)
                     /gene="pyrE"
                     /locus_tag="ACGEXS_000925"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFF8011947.1"
                     /translation="MAKEIAKSLLDIEAVTLSPNDLYTWSSGIKSPIYCDNRVTLGYP
                     LVRGAIRDGLINLIKEHFPEVEVISGTATAGIPHAAFIAEKLKLPMNYVRSSNKSHGK
                     QNQIEGAKSEGKKVVVIEDLISTGGSSVTAVEALKLAGAEVLGVVAIFTYGLKKADDT
                     FSNIQLPFYTLSDYNELIEVAENEGKISSEDIQTLVEWRDNLA"
     gene            complement(8892..9584)
                     /gene="pyrF"
                     /locus_tag="ACGEXS_000926"
     CDS             complement(8892..9584)
                     /gene="pyrF"
                     /locus_tag="ACGEXS_000926"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFF8011948.1"
                     /translation="MKDLPIIALDFESKEKVNQFLDLFDESLFVKVGMELFYQEGPQL
                     INEIKERGHDVFLDLKLHDIPNTVGKAMEGLAKLNVDLVNVHAAGGVKMMSEAIKGLR
                     KHNQDTKIIAVTQLTSTTEDMLRHEQNIQTSIEEAVLNYAKLANAAGLDGVVCSPLES
                     RMLTEKLGTSFLKVTPGIRPKGASQNDQHRITTPEEARQLGSTHIVVGRPITQSDNPV
                     ESYHKIKESWLV"
     gene            complement(9694..12867)
                     /gene="carB"
                     /locus_tag="ACGEXS_000927"
     CDS             complement(9694..12867)
                     /gene="carB"
                     /locus_tag="ACGEXS_000927"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFF8011949.1"
                     /translation="MPKRNDIKTILVIGSGPIIIGQAAEFDYAGTQACLALKEEGYRV
                     ILVNSNPATIMTDKEIADKVYIEPLTHDFIARIIRKEQPDALLPTLGGQTGLNMAIQL
                     HESGVLQDNNVQLLGTELTSIQQAEDREMFRTLMNDLNVPVPESDIVNTVEQAFKFKE
                     QVGYPLIVRPAFTMGGTGGGICHNDEELHEIVSNGLHYSPATQCLLEKSIAGFKEIEY
                     EVMRDKNDNAIVVCNMENIDPVGIHTGDSIVVAPSQTLSDVEYQMLRDVSLKVIRALG
                     IEGGCNVQLALDPHSFDYYIIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEM
                     LNPITGTSYAAFEPTLDYVISKIPRFPFDKFEKGERELGTQMKATGEVMAIGRTYEES
                     LLKAIRSLEYGVHHLGLPNGESFDLDYIKERISHQDDERLFFIGEAIRRGTTLEEIHN
                     MTQIDYFFLHKFQNIIDIEHQLKEHQGDLEYLKYAKDYGFSDKTIAHRFNMTEEEVYQ
                     LRMENDIKPVYKMVDTCAAEFESSTPYYYGTYETENESIVTDKEKILVLGSGPIRIGQ
                     GVEFDYATVHAVWAIQKAGYEAIIVNNNPETVSTDFSISDKLYFEPLTEEDVMNIINL
                     EKPKGVVVQFGGQTAINLADKLAKHGVKILGTSLENLNRAEDRKEFEALLRKINVPQP
                     QGKTATSPEEALANAAEIGYPVVVRPSYVLGGRAMEIVDNDKELENYMTQAVKASPEH
                     PVLVDRYLTGKEIEVDAICDGETVIIPGIMEHIERAGVHSGDSIAVYPPQTLTEDELA
                     TLEDYTIKLAKGLNIIGLINIQFVIAHDGVYVLEVNPRSSRTVPFLSKITDIPMAQLA
                     MRAIIGEKLTDMGYQEGVQPYAEGVFVKAPVFSFNKLKNVDITLGPEMKSTGEVMGKD
                     TTLEKALFKGLTGSGVEVKDHGTVLMTVSDKDKEEVVKLAQRLNEVGYKILATSGTAN
                     KLAEYDIPAEVVGKIGGENDLLTRIQNGDVQIVINTMTKGKEVERDGFQIRRTTVENG
                     IPCLTSLDTANALTNVIESMTFTMRQM"
     gene            complement(12860..13960)
                     /locus_tag="ACGEXS_000928"
     CDS             complement(12860..13960)
                     /locus_tag="ACGEXS_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFF8011950.1"
                     /translation="MQSKRYLVLEDGSFYEGYRLGSDNLTVGEIVFNTAMTGYQETIS
                     DPSYTGQIITFTYPLIGNYGINRDDFESLVPTLNGIVVKEASAHPSNFRQQKTLHDVL
                     ELHQIPGIAGVDTRSITRKIRQHGVLKAGFTDRKEDIDQLVKHLQQVELPKNEVEIVS
                     TKTPYVSTGKDLSVVLVDFGKKQNIVRELNVRGCNVTVVPYTTTAEEILAMAPDGVML
                     SNGPGNPEVVECAIPMIQGILGKIPFFGICLGHQLFALSQGASSFKMKFGHRGANHPV
                     KNLETGKVDITSQNHGYAIDIDSLKSTDLEVTHLALNDGTVEGLKHKTLPAFSVQYHP
                     EANPGPSDSNYLFDDFVAMMTNFKEKERHINA"
     gene            complement(13962..15236)
                     /locus_tag="ACGEXS_000929"
     CDS             complement(13962..15236)
                     /locus_tag="ACGEXS_000929"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFF8011951.1"
                     /translation="MKLIKNGKVLQNGELQQADILIDGKVIKQIAPAIEPSNGVDIID
                     AKGHFVSPGFVDVHVHLREPGGEYKETIETGTKAAARGGFTTVCPMPNTRPVPDSVEH
                     FEALQKLIDDNAQVRVLPYASITTRQLGKELVDFPALVKEGAFAFTDDGVGVQTASMM
                     YEGMIEAAKVNKAIVAHCEDNSLIYGGAMHEGKRSKELGIPGIPNICESVQIARDVLL
                     AEAAGCHYHVCHVSTKESVRVIRDAKRAGIHVTAEVTPHHLLLTEDDIPGNNAIYKMN
                     PPLRSTEDREALLEGLLDGTIDCIATDHAPHARDEKAQPMEKAPFGIVGSETAFPLLY
                     THFVKNGDWTLQQLVDYLTIKPCETFNLEYGTLKENGYADLTIIDLDSEQEIKGEDFL
                     SKADNTPFIGYKVYGNPILTMVEGEVKFEGDK"
     gene            complement(15254..16135)
                     /locus_tag="ACGEXS_000930"
     CDS             complement(15254..16135)
                     /locus_tag="ACGEXS_000930"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFF8011952.1"
                     /translation="MNHLLSMEHLSTDQIYKLIQKASQFKSGERQLPNFEGKYVANLF
                     FENSTRTKCSFEMAELKLGLKTISFETSTSSVSKGESLYDTCKTLESIGCDLLVIRHP
                     FNNYYEKLANINIPIANAGDGSGQHPTQSLLDLMTIYEEYGYFEGLNVLICGDIKNSR
                     VARSNYHSLKALGANVMFNSPNAWIDDSLEAPYVNIDDVIETVDIVMLLRIQHERHGL
                     AEETRFAADDYHQKHGLNEVRYNKLQEHAIVMHPAPVNRGVEIQSDLVEASKSRIFKQ
                     MENGVYLRMAVIDELLK"
     gene            complement(16163..17470)
                     /locus_tag="ACGEXS_000931"
     CDS             complement(16163..17470)
                     /locus_tag="ACGEXS_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFF8011953.1"
                     /translation="MQNDEMFERTVKPVLDVNEKPQPAQWAFLSLQHLFAMFGATVLV
                     PFLTGLPISAALLASGIGTLLYILITKAQIPAYLGSSFAFITPIITGLSTHSLGDMLV
                     ALFMSGVMYVIIGILIKLSGTAWLMKLLPPVVVGPVIMVIGLSLAPTAVNMAMYENPG
                     DMKGYNISFLIVAMITLLVTIVVQGFFKGFLSLIPVLVGIIVGYVVAIFMGIVKFDAI
                     MSAKWIDFPHIYLPFKDYVPSFHLGLVLVMIPIVFVTVSEHIGHQMVLNKIVGRNFFE
                     KPGLDKSIIGDGVSTMFASIIGGPPSTTYGENIGVLAITRIYSIYVIGGAAVIAIVLA
                     FIGKFTALISSIPTPVMGGVSILLFGIIAASGLRMLVESKVDFANNRNLVIASVILVV
                     GIGNLVFNLKEIGINLQIEGMALAALSGIILNLILPKEKKQNN"
     gene            complement(17688..18215)
                     /gene="pyrR"
                     /locus_tag="ACGEXS_000932"
     CDS             complement(17688..18215)
                     /gene="pyrR"
                     /locus_tag="ACGEXS_000932"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFF8011954.1"
                     /translation="MSERIIMDDAAIQRTVTRIAHEILEYNKGTDNLILLGIKTRGEY
                     LANRIQDKIHQIEQQRIPTGTIDITYFRDDIEHMSSLTTKDAIDIDTDITDKVVIIID
                     DVLYTGRTVRASLDAILLNARPIKIGLAALVDRGHRELPIRADFVGKNIPTSKEETVS
                     VYLEEMDQRNAVIIK"
     gene            complement(18615..19532)
                     /locus_tag="ACGEXS_000933"
     CDS             complement(18615..19532)
                     /locus_tag="ACGEXS_000933"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFF8011955.1"
                     /translation="METYEFNITDKEQTGMRVDKLLPELNNDWSRNQIQDWIKAGLVV
                     ANDKVVKSNYKVKLNDHIVVTEKEVVEADILPENLNLDIYYEDDDVAVVYKPKGMVVH
                     PSPGHYTNTLVNGLMYQIKNLSGINGEIRPGIVHRIDMDTSGLLMVAKNDIAHRGLVE
                     QLMDKSVKRKYIALVHGNIPHDYGTIDAPIGRNKNDRQSMAVVDDGKEAVTHFNVLEH
                     FKDYTLVECQLETGRTHQIRVHMKYIGFPLVGDPKYGPKKTLDIGGQALHAGLIGFEH
                     PVTGEYIERHAELPQDFEDLLDTIRKRDA"
     gene            complement(19532..20023)
                     /gene="lspA"
                     /locus_tag="ACGEXS_000934"
     CDS             complement(19532..20023)
                     /gene="lspA"
                     /locus_tag="ACGEXS_000934"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFF8011956.1"
                     /translation="MHKKYFIGTSILIAVFVVIFDQVTKYIIATTMKIGDSFEVIPHF
                     LNITSHRNNGAAWGILSGKMTFFFIITIIILIALVYFFIKDAQYNLFMQVAISLLFAG
                     ALGNFIDRILTGEVVDFIDTNIFGYDFPIFNIADSSLTIGVILIIIALLKDTSNKKEK
                     EVK"
BASE COUNT         6340 a         3820 c         2940 g         7164 t
ORIGIN      
        1 taaaattcgc actatttcaa tttgccattt aaaaatagtt tgtttaatca ttagtacttt
       61 tattcatcag taagtatggc gcttgcatta aaatcagaat tcgtaaatga aaatactaat
      121 aaattcctta tcaaaccaaa atcacgtaag ttataaagcc atataaaaag acagaatata
      181 aactgctaat ttaaatagtt aacagttgta tattctgccc tttttctgta cttaaagtaa
      241 atattcaaac actggttcaa atactcgtac atactgaact tgtttctaat aattagtaat
      301 gttacatcat catctgtgga tcaatatcaa tcttcaatgc taatttttct tttataaatt
      361 tttcatggta atagtcatct aaaaactgaa tggcttgtaa taatccaggt tcacttttat
      421 atttcactaa aatttggaat ctaaattcat tgttgattct cgcaagtgct gccggagatg
      481 gacctagtac aagcgctttt tctgttaaat gctgtaataa aattttatga acatgctgcg
      541 atgctaccat aaccttcttc atttctttat gtgagattgt gaaattaatc aaataataat
      601 acggtggata ctttcctaat ttacgatatt ccatttcctg acgataaaat gttaaataat
      661 catttttttg aacatccaat attgaataat gatctggatt atacgtttga atgatgactt
      721 gacctgcctt ttcatgacga ccagctctac cagccacttg cgttaatagt tgataagtac
      781 gttcgctcgc ccgaaaatca ggtaaattta acattgtatc tgcattcagc acaccaacta
      841 aagtaatatt tggataatct aatcctttcg caatcatctg agtacctagt aaaatgtcac
      901 cgttaccttt ttcgaattca gtcaataact tttcatgtgc acctttcttt gaggttgtat
      961 ctacatccat cctaattatg cgcgcatctt caaattcttg ttgcaatagt tcttcaactt
     1021 tctgagtacc agtgcctact tgtcgaatgt gttcactctc acaatttgga cattgattcg
     1081 gtggcgtctc ttggtaacca caatagtgac attttaataa gtctgtcgtt ttatgatacg
     1141 ttaatgaaat atcacagttt ggacattgcg gtacatatcc acaatcccga cataacataa
     1201 acgatgcata accacgtcga tttaaaaata aaacaacttg ttcctgtcga tctaatctta
     1261 attgtatggc ttcacgtaaa tcttttgaaa acattgaccg attaccttca ctcaattctt
     1321 cacgcatgtc tactatatca atttcaggta aagcttgttg gttcactctg tttggtaatg
     1381 atagcaaatg ataaacgcct ttttcagctc gtgcataact ttcaagacat ggtgttgcac
     1441 ttcctaaaat gactggacag tgatgatatt cacttcgcca ttgggcaatt tctctagcgt
     1501 gatatctcgg ataatcttct tgtttatatg tagattcatg ttcttcatca atgatgatta
     1561 accctaaatt tttgaaaggt gcgaacacac ttgaccttgc accaacactt actctcgcac
     1621 gaccatccct aattttttgc cattcatcat aacgttcccc attagataag ccagaatgta
     1681 atacagcaac gtcatcacca aatcgacgtt tgaagcgtaa aaccatttgc ggtgttagag
     1741 cgatttcagg aactaacatc atcgcctgtt ttccttggct taaaacatct tctatcgttt
     1801 gtaaatatac ttctgtttta cctgatcccg tcacaccatg aagtaaaaat gtttcttgct
     1861 cttggcttac aattttagct ttaatcgctt catatgcttt atattggtct tctgttaatt
     1921 gctgttttga ttcttgttcg aaaacacgat ctttaaatgg gtctctttca acaaccgcgt
     1981 catatttttc aacataacct tttttgataa gtccatctaa actggatttt gaaaagccca
     2041 tatcctcaat atcagttaaa aatattgttt tatgttgttc ttcagacaag taagcataca
     2101 aatcgtattg tttaataact ttctccaact tagctaatac ttcatcagga tgataccctt
     2161 caatgacacg aacagcacgc ttggtttttt tagttatatt ttgtgtgaga atcgtttttt
     2221 cttcaacgat atcatctttt aacaacttca taagcaattg aatatcatta tttttttgcg
     2281 catctttata ataatagtaa ccatgcttat caaatttttg taataaagct gaaggtagct
     2341 ctatgtcatc tttcatctta aatgcttttt tatacttcgc tttaatagca ctcggaagca
     2401 tcacttctag catagaaata cgtttaatga catgagttga acccatccac tcacttaaag
     2461 ctattaattc tgatgttaat tctggttgta tatctttcac ttctatgatt ttttttaact
     2521 tcgaaacgtc aagttgtgca tcaggttctg ctgttacttc cattacataa ccttgaatcg
     2581 ttcttggtcc aaaaggtaca attacacgca caccaggttg gatgacagat tcgagttgtt
     2641 cgggaattat ataatcaaat ttatagtcaa cgctcttcga cgcgacatcg actatgactt
     2701 tcgctatcat tattgccacc tagtttctag ttcatctaaa atttgtgcag ctaatactac
     2761 tttttttcct ttcttgatat ttactttttc attattttta aaatgcattg tcaattcatt
     2821 atcatcagaa ctaaatccga tagacatatc cccaacatta tttgaaataa tcacatctgc
     2881 atttttcttg cgtaattttt gttgtgcata attttcaata tcttcagtct ctgctgcaaa
     2941 gcctattaaa tactgtgatg ttttatgttc acctaaatat ttaagaatgt ctttagtacg
     3001 tttaaaagat actgacaaat caccatcctg ctttttcatc ttatgttcta atacatcaac
     3061 cggtgtatag tcagatacgg ctgctgcttt tacaacaata tcttgttcgt caaatcggct
     3121 tgtcacttgt tcaaacattt cttcagcact ttgaacatga ataacttcaa tatcttttgg
     3181 atcctctagt gttgtaggac cagcaactaa cgtcacgata gctcctcgat ttcgcaatgc
     3241 ttcagctatt gcatagccca tttttccaga agaacgattg gatacaaatc tgactggatc
     3301 gataacttca atagttggtc ctgctgtaac caatgcgcgt ttatcttgaa atgaactatt
     3361 agctaaacga ttactatttt gaaaatgagc atcaattaca gaaacgattt gaagcggttc
     3421 ttccatacgt cctttagcaa cataaccaca tgctagaaat ccgcttcctg gttcgataaa
     3481 atgataccca tcttctttta aaatattaat attttgctgc gtacgtttat tttcatacat
     3541 atgcacattc atagcaggcg caataaattt cggtgtctct gttgctagca acgttgatgt
     3601 caccaaatca tcagcaatac ctacactcaa ttttgcaatt gtatttgccg ttgcaggtgc
     3661 aacaatgatt gcatctgccc aatcacctaa tgcaatatgc tgtatttctg aaggattttc
     3721 ttctataaaa gtatctgtat aaacagcatt tcgacttatt gcttgaaatg ctaatggtgt
     3781 cacaaatttt tgtgcgtgat tcgttaacat aacgcgaact tcatacccag attgtgttaa
     3841 cttacttgtc aaatcaattg ctttatatgc cgcaatgcca cctgtaacgg ctaataatat
     3901 tttcttcata ttcaatctcc cttaaatatc actatgacat ttacgcttta catcatcata
     3961 tgcgcacaaa tgctcattac ttttttatag atacaaattt agtattatta taacatcaat
     4021 cattggataa actaaaaaaa cacacctaca taggtgcgtt tgatttggat atgccttgac
     4081 gtatttgatg tacgtctagc ttcacatatt tttaatggtc gaaactattc tttaccataa
     4141 taatcacttg aaataacagg gcgaatttta ccgtcagcaa tttcttctaa cgctctacca
     4201 actggtttaa atgaatgata ttcacttaat aattcagttt caggttgttc atcaatttca
     4261 cgcgctcttt tcgctgcagt tgttgcaatt aaatactttg atttaatttg tgacgttaat
     4321 tggtttaaag gtggatttaa cattattttt tagcctccaa aatcattttt ctatacttag
     4381 cttctacgcg ctctcttttt aagtgctcag cttctacaat acattgaatt ctattcttcg
     4441 caagttctac ttcatcatta actacaacgt aatcgtataa attcatcatt tcaacttctt
     4501 tacgcgcttc gttaatacga ctttgtattt tctcatcaga ttctgttcct ctacctacta
     4561 atcgctctct caagtgttct aaacttggag gtgctaagaa aataaatagc gcatctggaa
     4621 atttctttct aacttgcttt gcaccttcta cttcaatttc taaaaataca tcatgacctt
     4681 cgtccattgt atctttaaca tattgaactg gtgtaccata atagttgcct acatattcag
     4741 catattctat aaattggtca tctttgatta aagcttcaaa cgcatcccta gttttaaaaa
     4801 agtaatctac gccatcaact tcaccttcac gcatttgacg tgttgtcatt gaaatagaat
     4861 acttatatga tgtacttgga tcttcaaata ttcgttttct aacagtacct ttacctactc
     4921 cagatggtcc tgataaaacg attaacaatc ctttttcatt atccatgcct tacgacctct
     4981 ctaagctaat cttctattat ttaaatatga tatcacattg ttctttatat tgtatagcat
     5041 atttgaaatt gcatgccata atttctatta agtctaacaa tatcgttata ttgcacgatt
     5101 aattttaatt aaataaattg aattgcaaac ttttagataa tgtaaaatgt atggcataat
     5161 gtatggttca ataactatac tgaaaagtta caatcatgtt aaaatgaaac gaatgatatg
     5221 aagaaggtgg aagataaatt atggcttatg atggcttatt tacaaagaaa atggttgagt
     5281 ctctacaatt tttaacaaca ggacgcgttc acaaaatcaa tcaacctgat aatgacacga
     5341 tactaatggt tgtacgtcaa aatagacaaa accatcaatt gttattgtca atccatccaa
     5401 acttttcaag attacaattg actactaaaa aatatgataa tccatttaat ccacccatgt
     5461 ttgcgcgtgt ttttagaaaa cacttagaag gtggtattat cgaatcgatt aagcaaattg
     5521 gtaatgatcg tcgcattgaa atcgatataa agagtaaaga tgaaattggc gatactattt
     5581 accgcactgt catccttgag attatgggta aacatagtaa cttaatttta gtagatgaaa
     5641 atcgcaaaat aattgaagga tttaaacact taacaccaaa tacgaatcac tatcgtacag
     5701 taatgccagg atttaattat gaagcaccac ctactcagca caaaataaat ccgtatgata
     5761 ttacaggtgc agaggtgttg aaatatatcg attttaacgc aggtaatatt gctaaacaat
     5821 tattgaatca gtttgaagga tttagccctt taattacgaa tgaaatcgtt agtcgtcgtc
     5881 aatttatgac ttcatcaaca ttaccagaag catttgacga agtaatggca gaaaccaagt
     5941 taccacctac tcctattttt cataaaaatc atgaaacagg taaagaggat ttctatttta
     6001 taaagttaaa tcaatttaat gatgatacag ttacatacga ttcattaaat gatttgcttg
     6061 atcgttttta tgatgcgcgt ggcgaacgtg aacgcgttaa acaacgtgcg aatgatttag
     6121 ttcgatttgt tcaacagcag ttgcacaaat atcaaaataa attagcgaag ttgattgaag
     6181 aatatgagca gtctaaaaat aaagatactg aacagttata tggtgaattg atcactgcta
     6241 atatatatcg aattaagcaa ggcgataaag aagtgacggc attgaattat tatacgaatg
     6301 aagaagttgt cattccttta aatcctacaa aatccccatc agcaaatgct caatattatt
     6361 ataaacaata taatcgtatg aaaacgagag aacgtgaatt acaacatcaa attcaattga
     6421 cgaaagacaa tatagattat ttttcaacaa tcgaacaaca attacatcat atttctgtcc
     6481 atgacattga tgaaattaga gatgaattag cagaacaagg ctttatgaaa cagcgtaaaa
     6541 atcaaactaa gaaaaagaaa gcgcagattc aattacaaca ttatgtatca actgatggcg
     6601 acgatatata tgttggtaag aataacaagc aaaatgatta tttaacaaat aaaaaagcta
     6661 aaaaaactca cacatggtta cacacaaaag atattcctgg ttcacatgtc gttatattta
     6721 atgatgcacc aagtgatacg acaatcaagg aagcggctat gttagcagga tacttttcaa
     6781 aagctggtaa ttctggacaa atacctgttg attatacatt aattaaaaat gtgcataaac
     6841 catcaggtgc aaagcctggg tttgtaacat atgacaatca aaaaactttg tatgctacac
     6901 ctgattatga acttattcaa aaaatgaaac aatcataatc aagttgtcga caaataaaca
     6961 cctcacaaaa gaaagtagtc ctttcttatg gtgaggtgtt tatttattat ttaatgcgtt
     7021 cattgaaacg aagtatcgtg tttttaaata acgtatcatc atttaagtta caaattatct
     7081 ttgtgatttt aaaaatgagt gaaactaaaa tgctaatgtg tacttaaaat agaaatacat
     7141 aggtaaaacg catgcataag aaaaagtgtt tctttatcat tcaacgctgt gcgaatcctt
     7201 ttactcaggt aatgctaatt gaaaacttac tccaaactta tcttgaaccc aagcaaactc
     7261 tctgtatggt ggcatattcg tttttggcat taaaatggca ccttcatctt ttaatccatt
     7321 aaatagtcgt tccatttcaa tagtatcttt tactgtaaca aataatgaaa tcgcaggatt
     7381 cattggtaat tctgtgccac tattagcatc aatcgccatg aatacttgtc catttaatgt
     7441 aaatattgag tgttgtacag tcccgggatc accaggtcca ttttcaccat acttagccat
     7501 tgttataatc tcactatctt caaataagct tgtgtatagt ttaacagctt cttcagcttg
     7561 gttattaaac attaaaaatg tcgtgatttt tggaatatcc atgctatgcc tcctatagat
     7621 tgaacttcta taactactat tatcttaaca taattaaaat gacaaaagcg cgtcgtaccg
     7681 ttatgtgcac gaatgacaaa cgtcttattg gcctgttgat taaatcacat agttaacgat
     7741 aaaaattacg caagattaaa aatatgatac atatgttaat aaatgatact tatcataggt
     7801 attaatgttt tgacttattg tcaaatttga ttacaattaa aattaagtta aaggagggac
     7861 gttatgttac attatgtgat tccagtatta gtcctattac tcgtattctt aattggttgg
     7921 attttaataa agttaattat ccgctaataa attttttata taataaaagc tatagtgttt
     7981 gtaatattcg gatgcggtta agcagcccct gatattaaac actatagctt ttttgttttt
     8041 aaatttgtcc ttgtttacgt aatatatcct ttacatgttt aaataaatca tcattatgat
     8101 ctatagttgt tttatttttt atatgttttt gcttcgttga actttcgtcg acatcgtgac
     8161 tactagatag attggaatca tttgaatgat tgataaattc gttacaatgt tttatcttcc
     8221 catataaatc attagatgtt ttgtcattca tttgttgaat tcctccttct agtgtctata
     8281 ttatgctaag ttgtctctcc attcaactaa tgtttggata tcttcactag aaattttacc
     8341 ttcattttca gctacttcaa ttaattcatt gtaatcactt aaagtgtaaa aaggtagttg
     8401 aatattgcta aatgtatcat ctgctttttt caaaccgtaa gtaaagatag ctacaacacc
     8461 taatacttct gcacctgcta gttttaaggc ttcaactgct gtgactgaag atccccctgt
     8521 cgaaattaaa tcttctatca caactacttt tttaccttca cttttagcac cttcgatttg
     8581 attttgctta ccatgactct tatttgatga acgaacataa ttcattggta attttaattt
     8641 ttcagcaata aaagctgcat gtggaatacc agctgttgca gtaccagaaa taacttctac
     8701 ttcaggaaag tgttctttaa ttaagttaat taaaccatcg cggattgcgc ctcgaactaa
     8761 aggataacct aacgtaacac ggttatcaca gtaaatcggt gatttaatac ctgaactcca
     8821 tgtatataaa tcatttggtg ataatgttac agcttcaata tctaataatg attttgcaat
     8881 ttctttagcc attatactaa ccaactttct ttaattttat gataactttc gactggattg
     8941 tcactttgtg taatcggtct accgactaca atatgcgtcg aaccaagctg tcttgcttct
     9001 tccggtgtcg taatacggtg ttggtcattt tgagatgcac ctttaggtct aatacctggt
     9061 gttactttta aaaatgatgt acctaacttt tcagtcaaca tacgactttc aagaggtgaa
     9121 caaacaacgc catctaaacc agctgcattt gctaacttgg cataatttaa aacggcctct
     9181 tcaatcgatg tttgtatatt ttgttcgtgt cgtaacatgt cttctgttgt tgacgtaagc
     9241 tgtgttactg caataatttt tgtatcttga ttatgttttc ttaatccttt aatggcctca
     9301 gacatcattt ttacgccacc agcagcatga acatttacca gatcaacatt caatttagct
     9361 agtccttcca tcgccttacc aactgtatta ggaatatcat gcagttttaa atctaaaaat
     9421 acatcatggc ctctttcttt tatctcatta attaattgag gaccttcttg ataaaaaagt
     9481 tccataccta cttttacgaa taatgattca tcaaataaat ctaaaaattg atttactttt
     9541 tcttttgatt caaaatctaa tgcaataatt ggtaaatctt tcatcatata tgtctccctt
     9601 tatattaaga aattcattaa aataatgagg ctgtaatata aacttcttta agtaaatggt
     9661 taattacagc cccaccgata cagtttgatt gatttacatt tgacgcattg taaatgtcat
     9721 actttcaatt acattcgtta aggcattagc tgtatctaaa gatgtcaaac atggaatacc
     9781 attttcaact gtagtacgtc taatttggaa gccatccctt tctacttctt tacctttagt
     9841 cattgtattt ataacgattt gaacatcacc attttgaata cgtgttaata aatcattttc
     9901 gccaccaatt ttgcctacta cttctgcagg tatgtcatac tcagctaatt tattagctgt
     9961 tccagacgtt gctaaaattt tatagccaac ttcatttaag cgttgtgcca atttaacaac
    10021 ttcctcttta tctttgtcac tgacggtcat taatactgta ccgtgatctt taacttcaac
    10081 gccactacct gttaaccctt tgaataacgc cttttctaat gtagtatctt tccccatcac
    10141 ttcacctgtt gacttcattt caggtcctaa agtaatatca acatttttca atttattaaa
    10201 actaaatact ggtgctttca caaagacacc ctcagcatat ggttgaaccc cttcttgata
    10261 acccatatct gttagttttt ccccaatgat tgctcgcata gctaattgtg ccattggaat
    10321 atcagtaatt ttacttaaga atggtaccgt tctactagaa cgtggattta cttctaaaac
    10381 atacacacca tcgtgagcta taacgaattg aatgttgatt aagccaatga tgtttaaacc
    10441 tttagctaat tttatagtat agtcctcaag tgttgctaac tcgtcttctg tcaaagtttg
    10501 tggtggatat acagcgattg agtcaccact atgcacacca gcacgttcaa tatgttccat
    10561 gattcctgga ataatgaccg tttctccatc acaaatcgca tcaacttcaa tttctttacc
    10621 agttaaatat ctatcgacta gtaccggatg ttccggactc gcttttacag cctgggtcat
    10681 atagttttct aactctttgt cattgtctac aatttccatt gcgcgaccac ctaatacata
    10741 agaaggtctt actacaaccg gatatccgat ttctgcagca ttcgctaatg cttcctcagg
    10801 tgatgtagct gttttccctt gtggctgtgg cacgttaatt tttcttaata gtgcttcaaa
    10861 ttcttttcta tcttcagcac gatttagatt ttctagtgaa gtaccaagta ttttaacacc
    10921 atgtttagcc aatttgtctg ctaaattaat cgctgtttgt cctccaaatt gtacaacgac
    10981 acctttaggt ttttctaaat taatgatatt catcacatct tcttcagtta aaggttcaaa
    11041 gtataatttg tcagaaattg agaagtctgt tgaaactgtt tctggattgt tattcacaat
    11101 tatcgcttcg taccctgctt tttgaattgc ccaaacggcg tgaactgtcg catagtcaaa
    11161 ttctacacct tggccgattc gaattggtcc agagcctaat actaagattt tttctttgtc
    11221 agtaactatg gattcatttt cagtttcgta tgtaccataa taatatggtg ttgaagattc
    11281 aaattcagct gcgcaagtat caaccatctt gtaaacaggt ttaatatcat tttccatacg
    11341 caattgatat acttcttctt ccgtcatatt aaagcgatgc gctattgttt tatcactaaa
    11401 tccataatct tttgcatatt taagatattc taaatcacct tgatgctctt ttaattgatg
    11461 ctcaatatca ataatgtttt ggaacttgtg taagaagaag taatcaatct gagtcatatt
    11521 atgaatttct tctaatgttg tgcctcttct aattgcttcg ccgatgaaaa ataatcgttc
    11581 atcatcttgg tgtgaaatac gttctttaat ataatcaaga tcgaagcttt caccatttgg
    11641 taatcctaag tgatgcacac catactcaag tgatcgaatt gcttttaaca atgattcttc
    11701 gtaagttcga ccaatggcca taacttcacc tgttgctttc atttgtgtgc caagctctcg
    11761 ttctcctttt tcaaatttat caaaaggaaa tcttggtatt tttgaaatca catagtctaa
    11821 agttggttca aacgctgcat aagatgttcc tgtaattgga tttaacattt catctaatgt
    11881 tagaccaacc gcgattttag cagctaattt tgcaatagga tatcctgttg cttttgaagc
    11941 taacgctgat gaacgtgata cacgcggatt tacttctata atataataat cgaatgaatg
    12001 gggatctaat gctaattgaa cattacaacc accttcgata cctaaagctc gaataacttt
    12061 taatgaaaca tcacgtaaca tttgatactc aacatctgat aatgtttgac taggagccac
    12121 aacaattgaa tcgcctgtat gaataccaac tggatcaata ttttccatgt tacatacaac
    12181 gatggcatta tcgtttttat cacgcattac ttcgtattcg atttctttaa aacctgcgat
    12241 agatttttct aataaacatt gcgttgctgg actataatga agaccatttg agacgatttc
    12301 atgtaattct tcatcattat gacaaatacc gcctccggta ccacccatcg taaatgccgg
    12361 tctaacaatt agcgggtatc ccacttgctc tttgaattta aaggcttgct ctactgtatt
    12421 tacaatgtca ctctctggta caggaacgtt taaatcattc attaatgttc taaacatttc
    12481 acggtcttct gcttgttgaa ttgatgttag ctcagttcct aataattgga cgttattatc
    12541 ttgaagcaca ccactttcgt gtagttgaat cgccatgttt aaacctgttt gaccacctaa
    12601 agttggaagt aaagcgtcag gttgctcttt acgtataatt cgcgctataa aatcatgagt
    12661 taacggttcg atatatactt tatccgcaat ttccttatca gtcatgattg tcgctggatt
    12721 tgaatttaca agaataactc gatatccctc ttcttttaaa gctagacatg cttgtgttcc
    12781 agcataatca aattcagctg cttgacctat gataattggc ccagacccta ttactaaaat
    12841 tgttttgata tcattacgtt taggcattga tatgacgctc cttttcctta aaattagtca
    12901 tcattgctac aaaatcatca aatagatagt ttgaatctga cggtcctgga tttgcttcag
    12961 gatggtattg aacagaaaat gctggtaatg ttttatgttt taaaccttct acagtaccat
    13021 catttaatgc aagatgagta acttctaaat cagtactttt taacgaatct atatctattg
    13081 catatccatg gttttgactc gtaatatcaa cttttccagt ctctaaattt ttaactggat
    13141 ggttcgcacc acgatgacca aacttcattt taaatgagct tgctccttga gataatgcaa
    13201 aaagttgatg tcctagacag ataccaaaga acggaatttt ccctaaaatt ccttgaatca
    13261 ttggaatcgc acattctaca acttcaggat tacctggtcc gtttgatagc ataacgccat
    13321 ctggagccat tgctaaaatt tcttcggcag tagttgtata tggtacaact gtgacgttac
    13381 aacctctgac gtttaattct cgaacaatat tttgcttctt accaaagtct acaagtacga
    13441 cacttagatc cttacctgtc gaaacatacg gtgttttagt cgaaacgatt tctacttcgt
    13501 ttttaggtaa ttctacttgt tgtaaatgtt tgacaagttg atcaatatct tcttttcgat
    13561 cagtaaaacc agcttttaac acaccgtgtt gtcgaatttt acgcgtaata cttcttgtat
    13621 caacacctgc aatccctgga atttgatgca attctaaaac gtcatgaagt gtcttttgct
    13681 gtctaaaatt acttggatgc gcactcgctt ctttcactac aataccgttt aatgtaggta
    13741 ctaatgattc aaaatcgtct ctattgatac cataattacc gattaatgga tacgtaaaag
    13801 taatgatctg acctgtatat gatggatctg aaatagtttc ttgataacct gtcatcgctg
    13861 tattaaatac aatttctcct acagttaagt tatcagatcc taaacggtag ccttcgtaaa
    13921 aagaaccgtc ttctaacact agataacgtt tgctttgcat attatttatc cccctcaaat
    13981 ttaacttcgc cttcaaccat tgttaagatc ggatttccat aaactttata gccgataaat
    14041 ggtgtattat ctgcttttga taagaaatct tctcctttaa tttcttgttc actatctaaa
    14101 tcaatgattg ttaaatctgc ataaccattt tcttttaatg tgccgtattc taaattaaat
    14161 gtctcacatg gtttaattgt taagtaatct actaattgtt gtaatgtcca atcaccattt
    14221 tttacaaaat gcgtatataa taatgggaat gctgtttcac taccaacaat tccgaatggt
    14281 gctttttcca ttggttgtgc tttttcatca cgtgcatgtg gtgcatggtc tgttgcgata
    14341 cagtcaattg taccgtctag taacccttct aacaaagcct ctctatcttc agtacttctc
    14401 aatggtggat tcattttata aatggcatta ttaccaggaa tatcatcttc tgttaacaat
    14461 aaatggtgtg gtgtaacttc agctgtaaca tgaatgcctg ctcgtttagc gtcacgaatg
    14521 actctaacac tttctttagt agaaacatga catacatgat aatgacaacc tgctgcttca
    14581 gctaatagta catctcttgc gatttgaaca gattcacaaa tgtttggaat acctggtata
    14641 cctaactctt tactgcgttt cccttcatgc attgcaccac catagattaa tgaattatct
    14701 tcacagtgtg ctacgatggc tttgtttact tttgcagctt caatcatgcc ttcatacatc
    14761 atgcttgcag tttgtactcc tacaccgtca tctgtaaacg caaaggcacc ttcttttact
    14821 agtgctggga aatcaaccaa ttctttacct aattgacgtg ttgtaattga agcataaggt
    14881 aatacacgta cttgagcatt gtcatcgatt aatttttgta aagcttcaaa atgttctaca
    14941 gaatctggta ccggtcttgt gttaggcatt ggacatacag ttgtaaatcc gcctctagca
    15001 gcagctttag taccagtttc aattgtctct ttatattcac caccaggttc acgtaaatga
    15061 acatgaacat cgacaaatcc aggtgacaca aagtgacctt tcgcatctat gatgtcaaca
    15121 ccattgcttg gttcaattgc aggtgcaatt tgtttaatta ccttaccatc aattaaaata
    15181 tctgcttgtt gtaattcgcc attttgtaat actttaccgt ttttaattaa tttcatcatt
    15241 ttcgtcccct tacctatttt aataattcat caatgactgc cattcttaag taaacgccat
    15301 tttccatttg cttaaaaatt cttgattttg aagcttctac taaatcgctt tgtatttcta
    15361 ctcctctatt cacaggtgcc ggatgcataa caatagcatg ttcttgtaat ttgttatagc
    15421 gtacttcatt taagccatgc ttttgatgat aatcatctgc tgcaaatcta gtttcttctg
    15481 caagcccatg cctttcatgt tgaattctta ataacataac tatatctact gtttctataa
    15541 catcatctat atttacataa ggtgcttcta aagaatcatc aatccaagca tttgggctat
    15601 taaacattac gtttgcacct aatgctttta aactatggta attactacgt gcgacacgtg
    15661 aatttttaat gtctccacaa atcaatacat tcaagccttc aaaatatcca tattcttcat
    15721 atatcgtcat taaatcaagt aaactttgtg ttggatgttg tccactacca tcaccagcat
    15781 tcgcaattgg gatgttaata ttcgctaatt tttcatagta gttattaaac ggatgtctaa
    15841 tgactaataa atcacagcca atactttcta aagttttaca tgtgtcatat aaagattcac
    15901 cttttgaaac agatgatgtt gatgtttcaa agctaatcgt ttttaaccct agcttaagtt
    15961 ctgccatttc aaaactacat tttgttcgag tagaattttc aaagaataaa tttgcgacat
    16021 atttcccttc aaagtttggt agttgacgtt caccagattt aaattgactt gccttttgga
    16081 taagtttgta tatttgatct gtagataaat gttccattga taataaatga ttcataaaag
    16141 cgccctcctt aatttgtaaa tcttaattgt tttgtttttt ctctttaggt aagattaagt
    16201 tcaaaataat tcctgaaagt gcagctaatg ccatcccctc aatttgaagg ttgataccaa
    16261 tttcttttaa gttaaatact aaattaccga tacctacaac taaaattaca gaagctataa
    16321 ctaaatttcg attgttcgca aaatctactt tgctttcaac taacattctt aagccacttg
    16381 ctgcaataat accgaaaagt aatatagata ctcctcccat aactggtgta ggtatagaag
    16441 aaattaatgc agtgaactta ccaatgaatg ctaaaacaat tgctataact gctgcaccac
    16501 caatgacgta aatactgtat attctggtaa tcgctaatac accgatattt tcaccgtatg
    16561 ttgtacttgg tggtccacca ataatactgg caaacattgt agaaacacca tcaccaatga
    16621 ttgatttatc aagtcctggc ttttcaaaga agtttctacc tacgatttta ttcaatacca
    16681 tttggtgccc aatatgttca cttactgtta cgaacacaat cggaatcatt acaagtacaa
    16741 gtcctaagtg aaatgaaggt acataatctt taaatggcag ataaatatga gggaaatcta
    16801 tccattttgc tgacataatt gcatcaaatt tcacaatccc cataaaaatc gctaccacat
    16861 aacctacgat aatacctaca agaactggaa ttaaagatag gaatccttta aaaaatcctt
    16921 gaacgactat tgttacaagc aaggttatca ttgcaacaat taagaaactg atattgtaac
    16981 ctttcatatc tccaggattt tcatacattg ccatattgac tgcagtaggc gctaagctta
    17041 aaccaattac catgatgact ggtccaacaa caactggtgg taataatttc attaaccatg
    17101 ctgtcccact taatttgatt agaatcccga tgatgacgta cataacacca ctcatgaata
    17161 atgctacaag catgtctcct aagctatgcg tacttaatcc cgtgataatt ggcgtgataa
    17221 atgcaaagct agatcccaag tatgctggta tttgcgcctt cgttattaag atataaagta
    17281 atgtaccgat tcccgaagct agtaacgctg ctgatattgg tagtcctgtt aagaatggta
    17341 ctagtactgt tgcgccaaac atcgcaaata aatgttgtaa gcttaaaaat gcccattgcg
    17401 ctggttgtgg tttttcattt acatctagta cgggttttac tgttcgttca aacatttcat
    17461 cattttgcat aatattcatt tcctccgata ataaaaaaat ctctttacat cagtatatgt
    17521 aaagagacaa aaagtgtgac aagttgctac aagtcatttt cgtccataga aattgactta
    17581 tagttgtcga acatgagggt attattagat aaacaagcat atgaaaactt atttatcatt
    17641 caactccccc acctttttca gtctctcgta ctgaattaaa aggggtatta tttaattata
    17701 actgcatttc tttgatccat ttcttctaaa tagacactta ccgtttcctc tttagaagta
    17761 ggtatatttt taccaacaaa atctgctcga attggtaact cacgatgtcc tcgatcaacc
    17821 aaagcagcta aaccaatttt aataggtcta gcatttagca aaatagcatc aagtgaagca
    17881 cgaaccgttc gaccagtata cagcacatcg tcaataatga tgactacttt atctgtaata
    17941 tctgtgtcga tgtctattgc gtcttttgtc gtaagtgatg acatgtgctc tatatcatct
    18001 ctaaagtatg taatatcaat tgttccagta ggtatacgtt gttgctcaat ttgatgaatt
    18061 ttatcttgta tacgattcgc taaatattca cctcttgttt tgatacctaa aagaattaaa
    18121 ttatcagtac ctttgttata ctccaaaatt tcgtgagcga ttctcgtcac tgtacgttgt
    18181 attgcggcat catccattat gatacgttca gacatattct caccctttca aaatttagaa
    18241 acaaaaaacc tcatgtcttt aatatcgaca tgaggtttag tatatacaat cagcctattt
    18301 acatagaaga atcgtagtat tactacttat tcttgcatat gtgaaagtgt tatgatcatt
    18361 ttagtatata tctatatttt catgtctttg acgtctctct ggacgctcaa ttaaagactc
    18421 tttatgttgt acttaaactg ttataaggat aatactaatc aggcgctttg tcaatctata
    18481 ttgaaaaatt aaccatactc agtaaaaatg ttaaccttaa tctattttat aatgtgtttt
    18541 catttaaaaa aaacgaaaac gataaacact tatagtttac ataataatgt tatcgtaatt
    18601 atagcacgac acaattatgc atctcttttt cgaattgtat ctaataaatc ttcaaagtct
    18661 tgtggtaatt cagcatgtct ttcaatatat tcacctgtta ctggatgttc gaatccaata
    18721 agtccagcat gtagagcttg accaccaata tccaatgtct ttttcggtcc atactttgga
    18781 tcaccaacta atgggaagcc aatatatttc atgtgaacac ggatttgatg cgtacgtcct
    18841 gtttcaagtt gacattcaac aagcgtataa tctttaaaat gttctagtac gttaaaatgt
    18901 gtcactgctt ccttaccatc atcaacaaca gccatagatt gacgatcatt tttgtttcta
    18961 ccaattggcg catcgattgt accgtaatca tgaggaatat tcccgtgaac taaagcgata
    19021 tattttcttt taacagattt atccattaat tgttctacaa gcccacgatg agcaatatca
    19081 tttttagcaa ccattaataa accagaagta tccatatcta tacggtgaac aatacctgga
    19141 cgaatttctc cattaatacc tgacaaattt ttaatttgat acattaaacc attaactaat
    19201 gtattggtat aatgccctgg tgatggatga actaccatgc ctttcggttt atatacaact
    19261 gcaacatcgt catcttcata ataaatatct aaatttaaat tttcaggtag aatatcagct
    19321 tcaaccactt ctttttcagt gacaactata tgatcattaa gtttcacttt ataattagat
    19381 ttaacaactt tatcgtttgc aacgactaaa cctgctttaa tccaatcttg tatctggtta
    19441 cgagaccaat cattatttaa ttcaggcagc aacttatcta cacgcatacc tgtttgttct
    19501 ttatctgtaa tgttaaattc ataagtctcc attacttaac ctccttctcc tttttattgg
    19561 aagtatcctt taataaggca ataataatta atattacacc aattgttaaa cttgaatctg
    19621 cgatattaaa tattggaaaa tcataaccaa aaatatttgt atcaataaag tcaacaactt
    19681 ctcctgttaa aattctatca ataaagtttc caagtgcacc tgcaaaaagt aaactaatag
    19741 caacttgcat aaacaaatta tattgagcat ctttaataaa gaaatatact aaggctatta
    19801 atataataat ggtaataata aagaaaaatg tcatttttcc actcaatatt ccccatgcag
    19861 caccattatt tcgatgtgat gttatgttta aaaagtgcgg tatcacttca aatgaatctc
    19921 caattttcat tgtagtagct ataatatatt tagtaacttg gtcaaatata acgacaaata
    19981 ctgctattaa aatggaagtg ccaataaaat attttttgtg cattttcgtt cctccaatca
    20041 atcgtccatg agacaactct ttatattata gcttacacct gctaataaaa aaagtaagca
    20101 tattacatta aatctaatgt tactaactca atacttgata aactattatg ttttgacatt
    20161 aaatatgaac ttaattattc atttatcata tttaagatga cattaaaaat taggaaagca
    20221 ggctggaaca taaatcccta aaaagacagt agtaagatat tttc
//
LOCUS       DAXWFK010000033        20163 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.033, whole genome shotgun sequence.
ACCESSION   DAXWFK010000033 DAXWFK010000000
VERSION     DAXWFK010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20163
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.033"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(77..703)
                     /locus_tag="ACGEXS_000935"
     CDS             complement(77..703)
                     /locus_tag="ACGEXS_000935"
                     /EC_number="1.6.5.-"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050446 - azobenzene reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFF8011957.1"
                     /translation="MAKVLYITAHPFNELVSNSMAAGKAFIETYQQQHPDDEVKHIDL
                     FETYIPVIDKDVLTGWGKMSNGETLTDDEQMKVSRLSDILEEFLSADKYVFVTPMWNL
                     SFPPVVKAYIDAISIAGKTFKYSAEGPQGLLTDKKVLHIQSRGGYYTEGPAADFEMGD
                     RYLRTIMTFLGVPSYETIIIEGHNAEPHKTEEIKATSINNAEKLATTF"
     gene            912..1490
                     /locus_tag="ACGEXS_000936"
     CDS             912..1490
                     /locus_tag="ACGEXS_000936"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HFF8011958.1"
                     /translation="MTKRPKRILATIIIFLSLLFTIIYIDDIQKWFNQYTDKLTQNHK
                     GQGHSKWEDFFRGSRITETFGKYQHSPFDGKHYGIDFALPKGTPIKAPTNGKVTRIFN
                     NELGGKVLQIAEDNGEYHQWYLHLDKYNVKVGDRVKAGDIIAYSGNTGIQTTGAHLHF
                     QRMKGGVGNAYAEDPKPFIDQLPDGERSLYDL"
     gene            1873..2970
                     /locus_tag="ACGEXS_000937"
     CDS             1873..2970
                     /locus_tag="ACGEXS_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8011959.1"
                     /translation="MAELKLEHIKKTYDNNNTVVKDFNLHITDKEFIVFVGPSGCGKS
                     TTLRMVAGLESITSGDFYIDGERMNDVEPKNRDIAMVFQNYALYPHMTVFENMAFGLK
                     LRKVNKKEIEQKVNEAAEILGLTEYLGRKPKALSGGQRQRVALGRAIVRDAKVFLMDE
                     PLSNLDAKLRVQMRTEILKLHKRLNTTTIYVTHDQTEALTMASRIVVLKDGDIMQVGT
                     PREIYDAPNCIFVAQFIGSPAMNMLNATVEMDGLKVGTHHFKLHNKKFEKLKAAGYLD
                     KEIILGIRAEDIHEEPIFIQTSPETQFESEVVVSELLGSEIMVHSTFQGMELISKLDS
                     RTQVMANDKITLAFDMNKCHFFDEKTGNRIV"
     gene            2983..4254
                     /locus_tag="ACGEXS_000938"
     CDS             2983..4254
                     /locus_tag="ACGEXS_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin ABC transporter substrate-binding
                     protein"
                     /protein_id="HFF8011960.1"
                     /translation="MSKILKCITLAVVMLLIVTACGPNRSKEDIDKALNKDNSKDKPN
                     QLTMWVDGDKQMAFYKKITDQYTKKTGIKVKLVNIGQNDQLENISLDAPAGKGPDIFF
                     LAHDNTGSAYLQGLAAEIKLSKDELKGFNKQALKAMNYDNKQLALPAIVETTALFYNK
                     KLVKNAPQTLEEVEANAAKLTDSKKKQYGMLFDAKNFYFNYPFLFGNDDYIFKKNGSE
                     YDIHQLGLNSKHVVKNAERLQKWYDKGYLPKAATHDVMIGLFKEGKVGQFVTGPWNIN
                     EYQETFGKDLGVTTLPTDGGKPMKPFLGVRGWYLSEYSKHKYWAKDLMLYITSKDTLQ
                     KYTDEMSEITGRVDVKSSNPNLKVFEKQARHAEPMPNIPEMRQVWEPMGNASIFISNG
                     KNPKQALDEATNDITQNIKILHPSQNDKKGD"
     gene            4257..5525
                     /locus_tag="ACGEXS_000939"
     CDS             4257..5525
                     /locus_tag="ACGEXS_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFF8011961.1"
                     /translation="MTKRNPKLAALLSVIPGLGQFYNKRPIKGTIFFIFFISFISVFY
                     SFLNIGFWGLFTLGTVPKLDDSRVLLAQGIISILLVAFAIMLYIINILDAYRNAERFN
                     RNEEIKDPKARMVATWDKTFPYLLISPGTFLLIFVVVFPLIFMFGVAFTNYNLYNAPP
                     RHTLEWVGLDNFKTLFTIGVWRKTFFSVITWTLVWTLVATTLQIALGLFLAIIVNHPV
                     VKGKKFIRTVLILPWAVPSFVTILIFVALFNDEFGAINNDILQPLLGVAPAWLSDPFW
                     AKVALIGIQVWLGFPFVFALFTGVLQSISSDWYEAADMDGASSWQKFRNITFPHVIYA
                     TAPLLIMQYAGNFNNFNLIYLFNKGGPPVSGQNAGSTDILISWVYNLTFEFNNFNMGA
                     VVSLIIGFIVAIVAFIQFRRTSTFKDEGGL"
     gene            5527..6366
                     /locus_tag="ACGEXS_000940"
     CDS             5527..6366
                     /locus_tag="ACGEXS_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFF8011962.1"
                     /translation="MTKKKNILKAIGIYSFIAMMFVIILYPLLWTFGISLNPGTNLYG
                     AKMIPDNATFKNYAFLLFDDSSQYLTWYKNTLIVASANALFSVIFVTLTAYAFSRYRF
                     VGRKYGLITFLILQMFPVLMAMVAIYILLNTIGLLDSLFGLTLVYIGGSIPMNAFLVK
                     GYFDTIPKELDESAKIDGAGHMRIFLQIMLPLAKPILAVVALFNFMGPFMDFILPKIL
                     LRSPEKFTLAVGLFNFINDKYANNFTVFAAGAIMIAVPIAIVFLFLQRYLVSGLTTGA
                     TKG"
     gene            6440..7516
                     /locus_tag="ACGEXS_000941"
     CDS             6440..7516
                     /locus_tag="ACGEXS_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFF8011963.1"
                     /translation="MTIKVGIIGCGGIANGKHMPSLQKVENVEMIAFCDVDISKAASA
                     AEAYGTDNAKVYDDYKALLKDDTIDVIHVCTPNDSHCEITVAGLHAGKHVMCEKPMAK
                     TTAEAQKMIDTAKSTGKKLTIGYQNRFRADSQFLHQAAQRGDLGDIYFGKAHAIRRRA
                     VPTWGVFLDEEAQGGGPLIDIGTHALDLTLWMMDNYEPESVMGSTFHKLNKQHHAANA
                     WGSWNPDEFTVEDSAFGFIKMKNGATIILESAWAINSLEVDEAKCSLSGTKAGADMKD
                     GLRIHGEDMGTLYTKHVELENKGVDFYEGNEVDEAEEEAKAWIDAVVNDTEPVVKPEQ
                     AMVVTKILEAIYQSAKSGKAIYFE"
     gene            7541..8581
                     /locus_tag="ACGEXS_000942"
     CDS             7541..8581
                     /locus_tag="ACGEXS_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFF8011964.1"
                     /translation="MTKLKVGVIGVGGIAQDRHIPALLKLKDTVSLVAVQDINTVQMI
                     DVAKRFNIPHAVETPSELFKLVDAVVICTPNKFHADLSIEALNHGVHVLCEKPMAMTT
                     EECDRMIEAANKNHKLLTVAYHYRHTDVAITAKKAIESGVVGKPLVARVQAMRRRKVP
                     GWGVFTNKALQGGGSLIDYGCHLLDLSLWLLGKDMVPHEVLGKTYNQLSKQPNQINDW
                     GTFDHTKFDVDDHVTSYMTFANRASMQFECSWSANIKEDKVHVSLSGEDGGINLFPFE
                     IYEPRFGTIFESKANVEHNEDIAGERQARNFVNACLGIEEIVVKPEEARNVNALIEAI
                     YRSDLDNKSIQL"
     gene            8636..9604
                     /locus_tag="ACGEXS_000943"
     CDS             8636..9604
                     /locus_tag="ACGEXS_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFF8011965.1"
                     /translation="MKIGVFSVLFYDKNFEDMLDYVSESGLDMIEVGTGGNPGDKFCK
                     LDELLENEDKRQAFMKSITDRGLQISGFSCHNNPISPDPIEAKEADETLRKTIRLANL
                     LDVPVVNTFSGIAGSDDTAKKPNWPVTPWPTAYSEIYDYQWNEKLIPYWQDLAEFAKE
                     QDVKIAIELHAGFLVHTPYTMLKLREATNEYIGANLDPSHLWWQGIDPIAAIRILGQA
                     NAIHHFHAKDTYINQENVNMYGLTDMQPYGNVATRAWTFRTVGYGHSPYVWADIISQL
                     IINGYDYVLSIEHEDPIMSVEEGFQKACQTLKSVNIYDKPADMWWA"
     gene            complement(9965..10459)
                     /locus_tag="ACGEXS_000944"
     CDS             complement(9965..10459)
                     /locus_tag="ACGEXS_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HFF8011966.1"
                     /translation="MILSILLIFFCIRLVSLKISINHSKQLKADGAVEYGVKNSKFLA
                     ITHVLIYVLAGVEAFINKDTFSFANGIGLVILIFANIMLFMVIKTLGGIWTLKLFILP
                     NHPIIKSGLYKITKHPNYFLNIIPELIGVLLLTHATYTTILLVPYAYFLYVRIKQEEK
                     LMNI"
     gene            10692..12071
                     /gene="uhpT"
                     /locus_tag="ACGEXS_000945"
     CDS             10692..12071
                     /gene="uhpT"
                     /locus_tag="ACGEXS_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFF8011967.1"
                     /translation="MNFFDIHKIPNKGIPLSVQRKLWLRNFMQAFFVVFFVYMAMYLI
                     RNNFKAAQPFLKEEIGLSTLELGYIGLAFSITYGLGKTLLGYFVDGRNTKRIISFLLI
                     LSAITVLIMGFVLSYFGSVMGLLIVLWGLNGVFQSVGGPASYSTISRWAPRTKRGRYL
                     GFWNTSHNIGGAIAGGVALWGANVFFHGNVIGMFIFPSVIALLIGIATLFIGKDDPEE
                     LGWNRAEEIWEEPVDKENIDSQGMTKWEIFKKYILGNPVIWILCVSNVFVYIVRIGID
                     NWAPLYVSEHLHFSKGDAVNTIFYFEIGALVASLLWGYVSDLLKGRRAIVAIGCMFMI
                     TFVVLFYTNATSVMMVNISLFALGALIFGPQLLIGVSLTGFVPKNAISVANGMTGSFA
                     YLFGDSMAKVGLAAIADPTRNGLNIFGYTLSGWTDVFIVFYVALFLGMILLGIVAFYE
                     EKKIRSLKI"
     gene            complement(12430..13188)
                     /locus_tag="ACGEXS_000946"
     CDS             complement(12430..13188)
                     /locus_tag="ACGEXS_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFF8011968.1"
                     /translation="MFKVVICDDERIIREGLKQIIPWGDYHFNTIYTAKDGVEALSLI
                     QQHQPELVITDIRMPRKNGVDLLNDIAHLDCNVIILSSYDDFEYMKAGIQHHVLDYLL
                     KPVDHAQLEVILGRLVRTLLEQQSQNGRSLASCHDAFQPLLKVEYDDYYVNQIVDQIK
                     QSYQTKVTVSDLIQHIDVSESYAMRTFKDHVGITIVDYLNRYRILQSLQLLDRHYKHY
                     EIADKVGFSEYKMFSYHFKKYLQMSPSDYCKQAK"
     gene            complement(13181..14737)
                     /locus_tag="ACGEXS_000947"
     CDS             complement(13181..14737)
                     /locus_tag="ACGEXS_000947"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFF8011969.1"
                     /translation="MTAYKPYRHQLRRSLFASTIFPVFLVIIIGLVSFYAIYIWIEHR
                     TIHQHVDESQSSLHHTEKQIQTFITQHNNSFQELDLTNHHDVTATKRELLKLIHQQPA
                     TLYYELSGPNQFITNNYEHLNTKNMYLFSTHQLKFKNSTYMLKIYMANTPRLSEIKKD
                     NRQFALIVDQYDNILYANDDRFTIGEKYRPQQFGFMNESVKLNHADHRLIIYKDIHEN
                     IEDGITLLIVMAVVLVLLVIFGFISADNMAKRQTKDIETIIQKIYYAKNRHLGTYTPL
                     KNNSELEEINNYIYDLFESNEQLIHSIEHTERRLRDIQLKEIERQFQPHFLFNTMQTI
                     QYLITLSPKLAQTVVQQLSQMLRYSLRTNSHTVELNEELNYIEQYVAIQNIRFDDMIK
                     LHIESSEEARHQTIGKMMLQPLIENAIKHGRDTESLDITIRLTLARQNLHVLVCDNGI
                     GMSSSRLQYVRQSLNNDVFDTKHLGLNHLHNKAMIQYGSHARLHIFSKRNQGTLICYK
                     IPLSRGNVDV"
     gene            complement(14734..15702)
                     /locus_tag="ACGEXS_000948"
     CDS             complement(14734..15702)
                     /locus_tag="ACGEXS_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8011970.1"
                     /translation="MKSKIYILLLFLIFLSACANTRHSESDKNVLTVYSPYQSNLIRP
                     ILNEFEKQEHVKIEIKHGSTQVLLSNLHNEDFSERGDVFMGGVLSETIDHPEDFVPYQ
                     DTSVTQQLEDYRSNNKYVTSFLLMPTVIVVNSDLQGDIKIRGYQDLLQPILKGKIAYS
                     NPNTTTTGYQHMRAIYSMHHRVSDVHQFQNHAMQLSKTSKVIEDVAKGKYYAGLSYEQ
                     DARTWKNKGYPVSIVYPIEGTMLNVDGIALVKNAHPHPKRKKLVQYLTSRSVQQRLVA
                     EFDAKSIRKDVSEQSDQSIENLKNIPLIPKSKLPDIPHHKFLEMIQ"
     gene            16290..18539
                     /gene="pflB"
                     /locus_tag="ACGEXS_000949"
     CDS             16290..18539
                     /gene="pflB"
                     /locus_tag="ACGEXS_000949"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFF8011971.1"
                     /translation="MLETNKNHATAWQGFKNGRWNRHVDVREFIQLNYTLYEGNDSFL
                     AGPTEATSKLWEQVMQLSKEERERGGMWDMDTKVASTITSHDAGYLDKDLETIVGVQT
                     EKPFKRSMQPFGGIRMAKAACEAYGYELDEETEKIFTDYRKTHNQGVFDAYSREMLNC
                     RKAGVITGLPDAYGRGRIIGDYRRVALYGVDFLMEEKMHDFNTMSTEMSEDVIRLREE
                     LSEQYRALKELKELGQKYGFDLSRPAENFKEAVQWLYLAYLAAIKEQNGAAMSLGRTS
                     TFLDIYAERDLKAGVITESEVQEIIDHFIMKLRIVKFARTPDYNELFSGDPTWVTESI
                     GGVGIDGRPLVTKNSFRFLHSLDNLGPAPEPNLTVLWSVRLPDNFKTYCAKMSIKTSS
                     IQYENDDIMRESYGDDYGIACCVSAMTIGKQMQFFGARANLAKTLLYAINGGKDEKSG
                     AQVGPNFEGINSEVLEYDEVFKKFDQMMDWLAGVYINSLNVIHYMHDKYSYERIEMAL
                     HDTEIVRTMATGIAGLSVAADSLSAIKYAQVKPIRNEEGLVVDFEIEGDFPKYGNNDD
                     RVDDIAVDLVERFMTKLRSHKTYRDSEHTMSVLTITSNVVYGKKTGNTPDGRKAGEPF
                     APGANPMHGRDQKGALSSLSSVAKIPYDCCKDGISNTFSIVPKSLGKEPEDQNRNLTS
                     MLDGYAMQCGHHLNINVFNRETLIDAMEHPEEYPQLTIRVSGYAVNFIKLTREQQLDV
                     ISRTFHESM"
     gene            18562..19317
                     /gene="pflA"
                     /locus_tag="ACGEXS_000950"
     CDS             18562..19317
                     /gene="pflA"
                     /locus_tag="ACGEXS_000950"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFF8011972.1"
                     /translation="MLKGHLHSVESLGTVDGPGLRYILFTQGCLLRCLYCHNPDTWKI
                     SEPSREVTVDEMVNEILPYKPYFDASGGGVTVSGGEPLLQMPFLEKLFAELKENGVHT
                     CLDTSAGCANDTKAFQRHFEELQKHTDLILLDIKHIDNDKHIRLTGKPNTHILNFARK
                     LSDMKQPVWIRHVLVPGYSDDKDDLIKLGEFINSLDNVEKFEILPYHQLGVHKWKTLG
                     IAYELEDVEAPDDEAVKAAYRYVNFKGKIPVEL"
     gene            19653..>20163
                     /locus_tag="ACGEXS_000951"
     CDS             19653..>20163
                     /locus_tag="ACGEXS_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFF8011973.1"
                     /translation="MKRISKDIWAVFKLLYQNKGRFSINALLLQLIMIFISSTYLILL
                     FNMMLKVAGQSQLTINNWTEIVSHPASVILLIIFILSVAFLIYVEFSLLVYMVYAGFD
                     RQIITFKSIFKNAFVNVRKLIGVPVIFFVIYLMLMIPIANLGLSSVLTKNIYIPKFLT
                     EELMKTTKGI"
BASE COUNT         6749 a         3103 c         3846 g         6465 t
ORIGIN      
        1 tatttataaa aagcggaaaa gagatacaat aaaaaactgc atgacgtttg agacgtcaca
       61 cagtgtaact aaaaatttaa aaagttgttg ctaatttttc agcattatta atactagttg
      121 ctttaatttc ttcagtctta tgaggttcag cattgtgtcc ttcaataatg attgtttcat
      181 atgatggcac acctaagaat gtcataattg ttcttaaata acggtcaccc atttcaaaat
      241 cagcagcagg tccttcagta taatatccac cacgtgattg aatgtgtaat acttttttgt
      301 cagttagtaa accttgtggt ccttcagcag aatatttaaa agttttacct gcaattgaaa
      361 tagcatcaat atatgcttta actacaggtg ggaaagaaag gttccacata ggcgttacaa
      421 atacatattt atctgcactt aaaaattctt ctaaaatgtc actcaatctt gaaactttca
      481 tttgttcatc atcagttaac gtttcgccat tactcatttt tccccaacca gttaatacat
      541 ctttgtcaat aactggaata taagtttcaa ataaatcaat atgtttcact tcatcatcag
      601 gatgttgttg ttgatatgtt tcgataaatg ctttaccagc cgccatagaa tttgatacca
      661 gttcattaaa agggtgtgct gtaatatata atacttttgc catttgaaaa ttctcctctg
      721 tttctgttat tttcttaagt ataattatta tactcgatat aaaatttaat atcaatcaaa
      781 atattcaaat taccatcatt ttcttcatct atatttggca gtactactaa agtatgagtg
      841 catttaatta tgaaatagtt gatttagaat atatacttaa tacccaaaat atatgaagga
      901 tggatgccac tatgacaaag cgaccaaaac gtattttggc aacaattatc atttttcttt
      961 cactattatt tacgattatt tatatagatg acattcaaaa atggtttaac caatataccg
     1021 ataaattgac acaaaatcat aaaggacaag gacactcaaa atgggaagac ttttttagag
     1081 ggagtcggat tactgagact tttggtaaat atcaacattc accatttgat ggtaagcatt
     1141 atggcattga ttttgcattg ccaaaaggta caccaattaa agcgccgacg aatggtaaag
     1201 taacacgtat ctttaataat gaattgggcg gcaaggtatt acagattgcc gaagacaatg
     1261 gagaatatca ccagtggtat ctacacttag acaaatataa tgtcaaagta ggtgatcgag
     1321 tcaaagcagg tgatattatt gcatattcag gcaatacagg tatacaaacg acaggcgcac
     1381 atttacattt tcaaagaatg aagggtggcg taggtaatgc atatgcagaa gatccaaaac
     1441 cgtttatcga tcagttacct gatggggaac gtagcctata tgatttgtag ttatagaagg
     1501 gtgcccgcag tctaaaaaat taagcaatca ttgtgtgagt atgatactta cataatggtt
     1561 gcttttttca atgaaaatcg taatgctaag tcatacttgt ttgatttaga tattacttaa
     1621 aatgtaagac aaggttgtta gcattggcag tgaaatatcg cacataaaaa acattattgt
     1681 cacactagaa aatagttgtg cactatatca attttctgta taaaagttta attctgacag
     1741 taatgtaaac gtttacaatt tatgattgac attaataatg actgaatata tgatttatgt
     1801 aagtatttgt gcaaacgttt tcacaaagtg tattgcacaa tcaaactgta aacaaagtat
     1861 gggaggcata acatggcaga actaaagtta gagcatatta aaaagacgta tgataacaac
     1921 aatactgtag tgaaagattt taatctacat attactgaca aagaattcat tgtatttgtt
     1981 ggaccatcgg gatgtggtaa atcaacaaca ttacgaatgg ttgctggact agagtctatc
     2041 acatctggag atttttatat tgatggggaa cgcatgaacg atgttgaacc aaagaataga
     2101 gatattgcga tggtatttca aaactatgca ttatatccac atatgactgt ttttgaaaat
     2161 atggcatttg ggctaaagct acgtaaagta aataaaaaag agattgaaca aaaagttaat
     2221 gaagcagctg aaatattagg attaactgag tatcttggtc gtaaaccaaa agcgttatct
     2281 ggcggacagc gtcaacgtgt tgctttgggc agagctattg ttagggatgc gaaagtcttt
     2341 ttaatggatg aaccattatc gaatcttgat gcgaagcttc gagtacaaat gcgcacagaa
     2401 atattgaaat tacataagcg acttaatact acgacaattt atgttacaca tgatcaaact
     2461 gaagcattga cgatggctag tcgaattgtt gttttgaaag atggcgacat tatgcaagtc
     2521 ggcacaccta gagaaatata tgatgcccct aattgcatat ttgtggcgca atttatcggc
     2581 tcaccagcaa tgaatatgtt gaatgctaca gttgaaatgg acggattgaa ggtaggaaca
     2641 caccatttta aattacataa taaaaaattt gaaaagttaa aagctgctgg ctacttagac
     2701 aaggaaatta ttttaggtat tcgagctgaa gacattcatg aagaaccaat atttattcaa
     2761 acttctccag agacacaatt tgaatctgaa gtagttgtat ccgaactgtt aggttcagaa
     2821 attatggtac atagcacatt ccaaggaatg gaattgattt ctaaattaga ttcaagaact
     2881 caagtgatgg cgaacgacaa gattacacta gcatttgata tgaataagtg tcactttttt
     2941 gatgaaaaaa caggaaatcg tatcgtctaa gggggagtat tcatgtctaa aattttaaaa
     3001 tgtatcacgt tagccgtggt aatgttatta atcgtaactg catgtggccc taatcgttcg
     3061 aaagaagata ttgataaagc attgaataaa gataattcta aagacaagcc taaccaactt
     3121 acgatgtggg tggatggcga caagcaaatg gcgttttata aaaaaattac ggatcaatat
     3181 actaaaaaaa ctggcatcaa agtaaagctt gtaaatattg gtcaaaatga tcaactagaa
     3241 aatatttcgc tagacgctcc tgcaggaaaa ggtccagata tctttttctt agcacatgat
     3301 aatactggaa gtgcctatct acaaggctta gctgctgaaa tcaaattatc aaaagatgag
     3361 ttgaaaggtt tcaataagca agcacttaaa gcgatgaatt atgacaataa gcaactagca
     3421 ttgccagcta tcgttgaaac aaccgcactt ttttataata aaaaattagt gaaaaatgca
     3481 ccgcaaacgt tagaagaagt tgaagctaat gctgccaaac taactgatag taaaaagaaa
     3541 caatacggta tgttatttga tgctaaaaat ttctatttta attatccgtt tttattcggc
     3601 aatgatgatt atattttcaa gaaaaatggc agtgaatatg atattcatca gctaggacta
     3661 aattcaaaac atgtcgtcaa gaatgctgaa cgattacaaa aatggtacga caaagggtat
     3721 cttcctaagg cagcaacaca tgatgtcatg attggtcttt ttaaagaagg aaaagtagga
     3781 caatttgtca ctggaccgtg gaacattaat gaatatcaag aaacgtttgg taaagattta
     3841 ggagtaacaa cattacctac agatggtggc aaacctatga aaccatttct aggtgtacgt
     3901 ggttggtatt tatctgaata tagtaaacat aagtattggg ctaaagattt aatgctgtat
     3961 atcactagta aagatacatt acaaaaatat acagatgaaa tgagcgaaat tactggacgt
     4021 gttgacgtga aatcatctaa tccaaattta aaagtgtttg aaaagcaagc acgtcatgct
     4081 gaaccgatgc ctaatattcc tgaaatgcga caagtttggg aaccgatggg caatgcaagc
     4141 atatttattt caaatggtaa gaatcctaaa caagcgttag atgaggcgac gaatgatata
     4201 acgcaaaata ttaagattct tcatccatca caaaatgata agaaaggaga ttagttatga
     4261 cgaaacgtaa ccctaaatta gcggcattat tatctgttat acctggtttg ggacagtttt
     4321 ataataaaag acccattaaa gggacgatat tttttatctt tttcatcagt tttatttctg
     4381 ttttttatag ctttttaaat attggttttt ggggattgtt cacattaggg acagtaccta
     4441 agttagacga ttctcgtgtc ttacttgcac aaggtattat ttctatctta ctcgttgctt
     4501 tcgcaatcat gctatatatc attaatattt tagatgcata tcgtaatgct gaacgattta
     4561 atcgcaatga ggaaataaag gatccgaagg cgcgtatggt ggcaacatgg gacaagacgt
     4621 tcccatactt actaatctca ccaggtacat tcttattgat atttgtagtt gtatttccat
     4681 taatatttat gtttggagta gcatttacaa attacaattt atacaacgcg cctccgagac
     4741 acacattaga atgggttggt ttagataact ttaaaacgtt attcacaatt ggcgtttggc
     4801 gtaaaacatt tttcagtgtt attacttgga cattagtatg gacgcttgtt gcaacgacac
     4861 ttcaaattgc attagggctg tttttggcaa ttattgtaaa tcaccctgtc gtcaaaggta
     4921 agaaatttat ccgtactgtg ttaatcctac cttgggctgt accatcattt gtgacaattt
     4981 taatatttgt agcgttattt aatgatgaat ttggtgcgat aaataatgat attttgcaac
     5041 ctttattagg tgtagcacca gcatggttaa gtgatccgtt ttgggcaaaa gtggcattaa
     5101 tcggcattca agtatggctt ggattcccat ttgtctttgc actgttcact ggagtactgc
     5161 aaagtatttc atcagattgg tacgaagcag cagatatgga tggtgcgtct agttggcaaa
     5221 agtttagaaa catcacattc ccgcatgtca tttacgccac agcgccattg ttaattatgc
     5281 aatatgcagg taatttcaat aattttaatc ttatttatct atttaataaa ggcggtccac
     5341 cagtgtcagg gcagaatgct ggtagtacag atatcttgat atcttgggtg tataatctga
     5401 catttgagtt taacaacttc aacatgggtg cagttgtgtc attaattatt ggatttattg
     5461 ttgctattgt cgcatttatt caattcagac gtacaagtac gtttaaagat gagggaggtt
     5521 tataagatga caaagaagaa aaacatatta aaagcaatcg gtatttacag ttttatagcg
     5581 atgatgtttg tcatcatttt atatccacta ctgtggacat ttggcatttc ccttaatcca
     5641 ggtacgaact tgtatggtgc caaaatgata ccagacaatg caacatttaa aaattatgca
     5701 ttcttactat tcgatgacag tagtcaatac ctgacttggt ataaaaatac gcttatcgta
     5761 gcatctgcaa atgcactgtt tagtgtgata tttgtcacgt taacagcata tgctttttct
     5821 agatatcgct ttgttggtcg taaatacggg ctgattacat ttttgatttt acaaatgttc
     5881 cctgtattaa tggcaatggt cgcaatctat attttgctaa atacaattgg attattagat
     5941 tctttatttg gactaacact ggtatatatt ggtggatcaa taccgatgaa tgccttttta
     6001 gtgaaaggtt acttcgatac gattccaaaa gaacttgatg aatctgccaa aattgatggt
     6061 gcagggcata tgcgtatttt cttacaaatt atgcttccat tagctaagcc gattttagca
     6121 gttgttgctt tgttcaattt tatggggcca tttatggact ttatattacc taaaatacta
     6181 ttaagaagtc ctgaaaaatt cacattagca gttggattgt tcaactttat taatgataag
     6241 tatgcaaata atttcacagt gtttgcagca ggggcaatta tgattgcagt acctatagca
     6301 atcgtattct tgttcttgca acgctattta gtatcaggtt taacaacagg tgcgacaaaa
     6361 ggttagtttg aaattaggag tggggcagaa ttgataaaga accactaatg acgataaaga
     6421 ttaaaaggag gacgttatga tgacgattaa agttggaatc attgggtgtg gtggtattgc
     6481 gaatggcaag cacatgccaa gtttacaaaa agttgaaaat gttgaaatga tcgcattttg
     6541 tgacgtagac atttcgaaag cagcgagtgc ggcagaagca tacggaactg acaatgcaaa
     6601 ggtttatgat gattacaaag cattgttaaa agatgacacg attgatgtta tccatgtttg
     6661 tacgccaaat gactcgcatt gtgaaattac tgtagcaggg ttgcatgctg gtaaacatgt
     6721 gatgtgtgaa aaaccaatgg ctaaaacgac agcagaagct caaaaaatga tagatacagc
     6781 taaatcaaca ggtaaaaaat taacaatagg ttatcaaaat cgtttccgag cagatagtca
     6841 atttttacat caagcagcgc aacgtggcga cttaggagac atttacttcg gaaaggcaca
     6901 tgccattcgt cgtcgagcag taccaacatg gggtgtcttt ctagacgaag aagctcaagg
     6961 tggaggacca ttaatcgata tcggtacaca cgctttagat ttaacgttat ggatgatgga
     7021 taattatgaa ccagaatcag tgatgggttc aacattccat aaattaaata aacagcatca
     7081 tgcggcaaac gcttggggtt catggaatcc agatgaattt acagttgaag attctgcgtt
     7141 tggatttatt aaaatgaaga atggagcgac gatcatttta gaatccgctt gggcgattaa
     7201 ttctttagaa gtggatgagg caaaatgttc attatcagga actaaagcag gtgctgatat
     7261 gaaagatggt ctacgtattc atggtgaaga catgggtaca ctttatacca aacacgttga
     7321 attggaaaac aaaggcgtcg acttttatga aggtaatgaa gtggatgaag ctgaagaaga
     7381 agcaaaagct tggattgatg cagttgtaaa tgatactgaa ccagttgtga aaccggaaca
     7441 agcaatggta gttacaaaaa ttcttgaagc gatttatcag tctgcaaaat caggcaaagc
     7501 aatttacttt gaataacatc atacggtaag gaggcacatc atgacaaaat taaaagttgg
     7561 tgtgataggt gttggtggta ttgcacaaga ccgtcatatt ccagcattgc tgaaactcaa
     7621 agacacagtc tcattagttg cagtacaaga tattaataca gtgcagatga ttgatgttgc
     7681 gaagcgcttt aatatacctc atgcagttga gacacctagc gagctgttta aacttgttga
     7741 tgcggtggtc atttgtacac ctaataaatt ccatgctgat ctttctatag aagcattgaa
     7801 ccatggtgtc catgtattgt gtgaaaagcc aatggcgatg acgacggaag agtgtgatcg
     7861 catgattgaa gcggctaata aaaatcacaa attattaact gtcgcatatc attatcgtca
     7921 cacagatgtg gcaattactg ctaaaaaagc aattgaatca ggtgtggttg gtaaaccttt
     7981 agtagcacgt gtacaagcga tgcgtaggcg taaagtgcct ggctggggtg tttttaccaa
     8041 taaagcgttg caaggtggcg gtagtttaat cgattatggt tgccacttgt tagacttatc
     8101 tttgtggcta ctaggtaaag atatggtgcc gcatgaagtg ctaggaaaaa catataatca
     8161 attgagcaaa caaccgaatc aaattaatga ttggggaaca tttgatcata ctaaatttga
     8221 tgtcgatgat catgttacta gttatatgac atttgccaat cgagcaagca tgcagtttga
     8281 atgttcgtgg tctgcaaata tcaaagaaga taaggttcac gttagtttat caggagaaga
     8341 tggcggtatc aatttatttc catttgaaat atatgagccc cgctttggaa ctatttttga
     8401 aagcaaagct aatgttgagc ataacgaaga cattgctggt gagagacagg cgcgtaactt
     8461 tgtcaatgcg tgtttaggga tagaagagat tgtggtgaaa ccggaagaag cacgcaatgt
     8521 aaatgccctt atagaagcga tttatcgtag cgatcttgat aacaagagca tacaacttta
     8581 atgattatca tatatgatac aaaattctca atataaaaag aaggagtgct tttcaatgaa
     8641 aataggtgta ttttcagtat tattttacga taaaaatttt gaagatatgt tagattatgt
     8701 ctcagaatct ggattggata tgattgaagt tggaacaggt ggtaacccag gagataaatt
     8761 ttgtaagtta gatgagttgt tagaaaatga agacaagcgc caagcattta tgaagtcaat
     8821 cacagacaga ggcttacaaa taagtggttt cagttgtcat aacaatccaa tttctccaga
     8881 tccgatagaa gcgaaagaag ccgatgaaac gttacgtaaa acaatccgtt tagcaaatct
     8941 attagacgtg ccagttgtta atacattttc tggcattgca ggatcagatg ataccgctaa
     9001 aaagcctaat tggcctgtta caccttggcc aacagcctac tctgaaattt atgattatca
     9061 gtggaatgaa aagttgatac catattggca agatttagct gagtttgcaa aagagcaaga
     9121 tgtaaaaatt gccatagagt tgcatgcagg atttttagtg catacaccat atacaatgtt
     9181 gaagttacgt gaggctacaa atgaatatat cggtgctaac ttagatccta gtcatctatg
     9241 gtggcaaggt attgacccaa ttgctgcgat tcgcatatta ggccaagcaa atgcaattca
     9301 tcacttccat gctaaagata cgtatattaa tcaagaaaat gtaaatatgt atggtctaac
     9361 tgatatgcaa ccatatggta acgttgcgac aagagcatgg acattccgta cagttggtta
     9421 tggacatagt ccatatgtat gggcagatat cataagtcaa cttattatta atggatatga
     9481 ttatgtatta agtattgaac atgaagatcc tattatgtca gtagaagaag gtttccaaaa
     9541 agcttgtcaa actttgaaat ctgttaatat ttacgacaag ccagcagaca tgtggtgggc
     9601 ataatacgaa ctcgaggtta gtctgaagtt tgtctgaagt aagactggtg gcagtgttga
     9661 ataaatgcat atgtcgccaa gccattgcca aaaatttcac accttaaatc aagtcattgt
     9721 ttgtaaagaa ggtgtacttt atataagtat atagcgatgg tcatacccat tcacagtaac
     9781 aatcctcacc attgaaaaga gtatataacc ttttcaatag tgaggtatat gataataaaa
     9841 aaagcctgtt gtcacaatgg tcatagacac gacatacttt aaaggtttct gaatataata
     9901 tttcagaatg cactttaaag atggacgtcg atgtagacta aagtgatgac aggctttcat
     9961 ctttttaaat attcattaat ttctcttctt gtttaatacg tacatataag aaatacgcat
    10021 acggtactaa taaaatagtt gtatatgttg cgtgtgttaa taataataca ccgattaatt
    10081 caggaatgat gtttaagaag taatttgggt gttttgtaat tttatataat ccagatttaa
    10141 taataggatg gttaggtaaa atgaataatt ttaatgtcca aataccacct aaagttttaa
    10201 taaccataaa taacatgata ttagcaaaga ttaatataac taagccaata ccatttgcaa
    10261 agctaaatgt atctttatta ataaatgcct ctacaccagc caatacataa attaaaacgt
    10321 gtgttattgc taaaaacttc gaatttttaa cgccatattc aactgcaccg tctgctttta
    10381 attgttttga gtgattaata gatatcttta agctgacaag tctgatacag aaaaagataa
    10441 gtaatataga tagaatcatg atgtcctccg tcattatgtc atatgtataa gcgttgattt
    10501 tgacaacata aagtatttta tagataaagc ttgtcaaata ctattaacta tttattaatt
    10561 ttagtacata aatatgtttc taagtatgtg tttatgttca gtattttgga taatttaata
    10621 attttaagga tattaagcgc ttacaccgac gtgatatatt tggcttaacg aaaatgattg
    10681 aggtgacaga gatgaacttt tttgatatcc ataagattcc gaacaaaggc attccattat
    10741 cggtacaacg taaattatgg cttagaaact tcatgcaagc tttcttcgta gtgttctttg
    10801 tttatatggc tatgtattta attcgaaaca actttaaggc ggcacaaccg tttttaaaag
    10861 aggaaattgg attatctaca ttagaacttg gttatatcgg attagcattt agtatcacgt
    10921 acggtttagg aaaaacatta cttggatatt ttgtcgatgg acgtaacaca aaacgtatta
    10981 tctcgttctt acttatctta tctgcgatta cagttttaat tatgggattt gttttaagtt
    11041 actttggttc tgtaatggga ttattaattg tactttgggg acttaacggg gtgttccaat
    11101 cagttggtgg acctgcaagt tattcaacga tttcaagatg ggcgccaaga acgaaacgtg
    11161 gccgatactt aggattctgg aatacatcac ataatatcgg tggtgccata gcaggtggtg
    11221 ttgcactttg gggtgctaat gtattcttcc atggaaatgt tatagggatg ttcattttcc
    11281 catcggtgat tgcattactt attggtatcg caacattatt tatcggaaaa gatgatccgg
    11341 aagaattagg atggaatcgt gctgaagaaa tttgggaaga gccggtcgat aaagaaaata
    11401 ttgattctca aggtatgacg aaatgggaga tctttaaaaa atatatcctg ggaaatcctg
    11461 ttatatggat tctatgtgtt tcaaacgtct ttgtatacat tgtacgaatc ggtattgata
    11521 actgggcacc gttatatgtg tcagagcatt tacactttag taaaggcgat gcagttaata
    11581 cgatattcta ctttgaaatt ggtgcattag ttgcaagttt attatggggc tacgtatcag
    11641 acttattaaa aggtcgtcgt gcaattgtag ctattggctg tatgtttatg attacatttg
    11701 ttgtcttatt ctacacaaat gctacaagtg tcatgatggt taacatttca ttgtttgcat
    11761 taggtgcgtt aatctttggt ccgcaattat taattggtgt atcattgact ggttttgttc
    11821 ctaaaaatgc catcagtgta gcaaacggaa tgacaggttc attcgcgtat ctattcggtg
    11881 actcaatggc gaaagttggt ttggcggcta ttgctgatcc aacacgtaac ggtttaaaca
    11941 tctttggata tacattaagt ggatggacag atgttttcat cgtcttctat gttgcattat
    12001 tcctaggcat gattctatta ggaatcgttg ctttctatga agaaaagaaa attagaagtt
    12061 taaaaattta atataaatcg gattaaaagt atcgccaatc tattgcaata tagttggcaa
    12121 tcctgccccg acggcatgtg cgtgaagaga tgaaagatac tgcttctacc cttgcaaata
    12181 tatcatctct atgtctcggg gcagatcata attccctgtt atgaagtatc cttatttgcc
    12241 cgacttaggg tgactcaatg aatttactcc ttacaataaa gacatatagc ggtgtcaata
    12301 ttgtagggag tattgtttta tatttaaact ctctaaaaag cggactgaaa gaaaagtgaa
    12361 aacttctcta tcagtccgct ttttcataga acaaaatgga ggcgccataa tcattagtta
    12421 tgtgctaatc tattttgctt gcttacaata atcacttggc gacatttgta aatatttttt
    12481 aaaatgatag ctaaacattt tatactctga aaagcctact ttgtctgcaa tttcatagtg
    12541 tttgtaatgt cgatctaaca attgcagaga ttgtaaaata cgatagcgat ttaaataatc
    12601 gacaattgta ataccaacat gatctttaaa tgttcgcatc gcatacgatt cactaacatc
    12661 gatatgttga attaaatctg aaacagtcac tttcgtttga taagattgct taatttgatc
    12721 cacaatctgg tttacataat aatcatcgta ttctactttt aatagtggtt ggaaggcatc
    12781 atgacaagat gctaagctac ggccgttctg tgattgttgc tctaataagg tacggacaag
    12841 tcttcctaaa ataacttcta attgtgcatg gtctactggt tttaataaat aatcaagaac
    12901 atgatgttga atgccggctt tcatatattc aaagtcatcg taactcgata atatgatgac
    12961 attacaatct agatgcgcaa tatcattgag taaatcgacg ccatttttac gtggcatacg
    13021 aatatcagta attactaatt ctggctgatg ttgttgaatt agtgataatg cttcaacacc
    13081 atctttagca gtgtatattg tattgaaatg atagtctccc caaggaatga tttgctttaa
    13141 tccttctcga ataattcgtt catcatcaca aataactacc ttaaacatct acattccccc
    13201 ttgaaagtgg tattttataa caaattaacg taccttgatt acgctttgaa aaaatatgga
    13261 gtcgtgcatg tgaaccatat tgaatcattg ctttattgtg taaatgattt aatcccaaat
    13321 gcttagtatc aaatacatca ttattaagag attggcgtac atattgcagg cgagatgacg
    13381 acatcccgat accattgtcg caaactaaaa catgtaaatt ctgacgtgcc aatgtcaggc
    13441 gtatagtaat gtccaatgac tcagtatctc taccatgttt aatagcattt tctatgagtg
    13501 gctgaagcat cattttacca attgtctggt gacgcgcttc ttcagaactt tcaatatgga
    13561 gcttaatcat gtcatcaaaa cggatgtttt gtattgcaac atactgttca atgtagttca
    13621 actcttcgtt taattccact gtatgtgagt ttgtacgtaa tgagtaacgt aacatttgcg
    13681 ataattgttg gaccacagtt tgtgctaatt tcggagataa cgtaattaaa tattgtattg
    13741 tttgcatcgt attgaatagg aaatgaggct ggaattggcg ttctatttcc tttaactgaa
    13801 tatcacgcaa gcgacgttct gtatgctcga tagaatggat cagttgctca tttgattcaa
    13861 ataaatcgta aatataatta ttaatttctt ctagttcact gttgtttttt aaaggcgtat
    13921 atgtacctag atgacgattt ttggcatagt aaattttttg aataatcgtt tcgatatctt
    13981 ttgtttgtcg tttagccata ttatctgcgc taatgaaacc aaatattact agtaaaacaa
    14041 gaactacggc cataacaatt aacaacgtga taccatcttc aatgttttca tgtatatctt
    14101 tataaataat gagacgatgg tcagcatggt ttaattttac agattcattc ataaatccga
    14161 attgttgtgg tctatacttt tcacctatag taaaacggtc atcgttggcg tataaaatat
    14221 tgtcatattg atcaacgata agtgcgaatt gtcggttatc tttcttaatt tcacttaaac
    14281 gtggggtgtt agccatataa attttaagca tatatgtact atttttgaat ttaagctgat
    14341 gcgttgaaaa taaatacata tttttagtgt ttaaatgttc ataattattg gttataaact
    14401 gatttggtcc agataattca taataaagtg ttgcgggctg ttggtgtatt aattttaata
    14461 attcacgttt tgtagcggtc acatcatgat gatttgttaa atcgagctct tgaaacgaat
    14521 tattatgctg tgtaataaat gtctgaatct gcttttcagt atgatgtaaa gatgactgac
    14581 tttcatcaac atgttgatga atcgtacgat gctcaatcca aatatagatg gcatagaagc
    14641 ttactagtcc aataataatg actaaaaata ctggaaaaat agtagacgca aataacgatc
    14701 gtcttaattg atgtctataa ggtttgtatg ccgtcattga atcatctcca aaaatttatg
    14761 atgtggaata tccggtaatt tagatttcgg tattaaaggt atgttcttaa gattttcgat
    14821 agactgatcg ctttgttcac taacatcctt tcgaattgac ttggcatcga actctgcaac
    14881 taatcgttgt tgtactgagc ggcttgttaa atattgcact aactttttac gcttaggatg
    14941 agggtgtgca tttttaacta aagcaatacc atcaacattt aacattgttc cttcaattgg
    15001 ataaacgatt gatacaggat aacctttgtt tttccatgtg cgtgcatctt gttcgtagct
    15061 tagacctgcg taatatttac cttttgcaac atcttcaatg actttagacg tctttgacag
    15121 ttgcatcgca tggttttgga attgatgcac atcacttact cgatgatgca tgctataaat
    15181 agcacgcata tgttgatagc ctgtcgttgt tgtatttgga tttgagtacg caattttacc
    15241 tttaagtata ggttgtaata aatcttgata acctcgaatc ttaatatctc cttgtaaatc
    15301 tgaattcact actataactg ttggcattaa tagaaaacta gtaacatatt tattgttcga
    15361 gcgataatcc tctaattgct gtgttacaga tgtatcttga tagggaacaa aatcttctgg
    15421 atgatcaatt gtttctgaca acacaccacc cataaagaca tcaccacgct ccgaaaaatc
    15481 ttcgttatgc aagtttgaaa gcagtacttg agtagatccg tgtttaattt caattttgac
    15541 atgctcttgt ttttcaaatt catttaaaat tggacgaatc aagtttgatt gatacggaga
    15601 ataaactgtt aatacatttt tatcggattc agagtgacgc gtattagcgc atgctgataa
    15661 aaaaatgaga aataatagca agatataaat ttttgatttc atgatatccc atcaattcta
    15721 tgtatatttt aatacaataa ttttagcaat aaatgacgca taagtaatgt taaatattta
    15781 gaaatgttta tagatgactt gttaagacgt tgcaaatgtt gtgatagcac aaaatttttg
    15841 tttgtcaaga cgatttaccg aggctgtaaa atcaaactgt tatattttat ttgtagctgt
    15901 tatataaaaa tcggcaagat attgaacggt tcaaaagtga atttttacgt caataaaagt
    15961 atttaatcca gtctcttcat atataaaagt aaatctttct aagtgttgat ttaacgctta
    16021 tcaacaatca ttttttataa acaaatatat actcctaaat taacttttaa agcaatgaaa
    16081 atagtgaaca ttataactgt tgtgtaacag aatgcaatta gcatattact gttacacaaa
    16141 ttagtacagt ttctatgttt tgacatacat ttgatgaaaa ttgtacataa tttatgtgaa
    16201 aaaaatcaca acaaacatgc tacaatgact atgaaaacgt taacatagca tttcaaattc
    16261 acaacattat acagatggag gcgtttagta tgttagaaac aaataaaaat catgcaacag
    16321 cttggcaagg atttaaaaat ggaagatgga acagacacgt agatgtaaga gagtttatcc
    16381 aattaaacta cactctttat gaaggtaatg attcattttt agcaggacca acagaagcaa
    16441 cttctaaact ttgggaacaa gtaatgcagt tatcgaaaga agaacgtgaa cgtggcggca
    16501 tgtgggatat ggacacgaaa gtagcttcaa caatcacatc tcatgatgct ggttatttag
    16561 acaaagattt agaaacaatt gtaggtgtac aaactgaaaa gccattcaaa cgttcaatgc
    16621 aaccattcgg tggtattcgt atggcgaaag cagcttgtga agcttacggt tacgaattag
    16681 acgaagaaac tgaaaaaatc tttacagatt atcgtaaaac acataaccaa ggtgtattcg
    16741 atgcatattc tagagaaatg ttgaactgcc gtaaagcagg tgtaatcact ggtttacctg
    16801 atgcatacgg acgtggacgt attatcggtg actatcgtcg tgtagcttta tatggtgtag
    16861 atttcttaat ggaagaaaaa atgcacgact tcaacacgat gtctacagaa atgtcagaag
    16921 atgtaattcg tttacgtgaa gaattatcag aacaatatcg tgcattaaaa gaattaaaag
    16981 aacttggaca aaaatatggt ttcgatttaa gccgtccagc agaaaacttc aaagaagcag
    17041 ttcaatggtt atacttagca taccttgctg caattaaaga acaaaacggt gcagcaatga
    17101 gtttaggtcg tacatcaaca ttcttagata tctatgctga acgtgacctt aaagcaggcg
    17161 ttattactga aagcgaagtt caagaaatta ttgaccactt catcatgaaa ttacgtattg
    17221 ttaaatttgc tcgtacacct gattacaatg aattattctc tggagaccca acttgggtaa
    17281 ctgaatctat cggtggtgta ggtattgacg gacgtccact tgttacgaaa aactcattcc
    17341 gtttcttaca ctcattagat aacttaggtc cagctccaga accaaactta acagtattat
    17401 ggtcagtacg tttacctgac aacttcaaaa catactgtgc aaaaatgagt attaaaacaa
    17461 gttctatcca atatgaaaat gatgacatta tgcgtgaaag ctatggcgat gactatggta
    17521 tcgcatgttg tgtatcagcg atgacaattg gtaaacaaat gcaattcttc ggtgcacgtg
    17581 cgaacttagc taaaacatta ctttacgcta tcaatggtgg taaagatgaa aaatctggtg
    17641 cacaagttgg tccaaacttc gaaggtatta acagcgaagt attagaatat gacgaagtat
    17701 tcaagaaatt tgatcaaatg atggattggc tagcaggtgt ttacattaac tcattaaatg
    17761 ttattcacta catgcacgat aaatacagct atgaacgtat tgaaatggca ttacatgata
    17821 cagaaattgt acgtacaatg gcaacaggta tcgctggttt atcagtagca gctgactcat
    17881 tatctgcaat taaatatgca caagttaaac caattcgtaa cgaagaaggt cttgtagtag
    17941 actttgaaat cgaaggcgac ttccctaaat acggtaacaa tgacgaccgt gtagatgata
    18001 ttgcagttga tttagtagaa cgcttcatga ctaaattacg tagtcataaa acatatcgtg
    18061 attcagaaca tacaatgagt gtattaacaa ttacttcaaa tgttgtatac ggtaagaaaa
    18121 ctggtaacac accagacgga cgtaaagctg gcgaaccatt tgctccaggt gcaaacccaa
    18181 tgcatggccg tgaccaaaaa ggtgcattat cttcattaag ttctgtagct aagatccctt
    18241 acgattgctg taaagatggt atttcaaata cattcagtat cgtaccaaaa tcattaggta
    18301 aagaaccaga agatcaaaac cgtaacttaa ctagtatgtt agatggttac gcaatgcaat
    18361 gtggtcacca cttaaatatt aacgtattta accgtgaaac attaatagat gcaatggaac
    18421 atccagaaga atatccacag ttaacaatcc gtgtatctgg ttacgctgtt aacttcatta
    18481 aattaacacg tgaacaacaa ttagatgtaa tttctcgtac attccatgaa agtatgtaac
    18541 aaaatttaag gtgggagcac tatgcttaag ggacacttac attctgtcga aagtttaggt
    18601 actgtcgatg gaccgggatt aagatatata ttatttacac aaggatgctt acttagatgc
    18661 ttgtattgcc acaatccaga tacttggaaa attagtgagc catcaagaga agtcacagtt
    18721 gatgaaatgg tgaatgaaat attaccatac aaaccatact ttgatgcatc gggtggcggt
    18781 gtaacagtca gtggtggcga accattgtta caaatgccat tcttagaaaa attatttgca
    18841 gaattaaaag aaaatggtgt gcacacttgc ttagacacat cggctggatg tgctaatgat
    18901 acaaaagcat ttcaaaggca ttttgaagaa ttacaaaaac atacagactt gatattatta
    18961 gatataaaac atattgataa tgacaaacat attagattga caggaaagcc taatacacac
    19021 atccttaact tcgcgcgcaa actgtcagat atgaaacaac ctgtatggat tcgacatgtc
    19081 cttgtgcctg gttattctga tgataaagac gatttaatta aactagggga atttattaat
    19141 tctcttgata acgtcgaaaa gtttgaaatt ctgccatatc atcagttagg tgttcataag
    19201 tggaaaacat tgggcattgc atatgaatta gaagatgtcg aagcgcccga tgatgaagct
    19261 gttaaagcag cctaccgtta tgttaacttc aaagggaaaa ttcccgttga attataaata
    19321 caattcagac cgaaaagaaa gcatatgcaa cttcaagagt gaaggggcat atgcttcttt
    19381 ttcaattgag tattgagtat tagcaagacg tagtaagtat atgagacaac ttctacaatg
    19441 gttgaaggaa gacgtttttg taagtagcta tgctgataaa gaatgtgatg tcttgttaaa
    19501 ggtggggttc caatatcatc atttagctga tgttgaatgg gttattattt gctacttgca
    19561 tatgaatatg agtcttttca aatttttatt gaccctgagt aatgaaaaat attaagatga
    19621 aacttaatat taaagcaatg cggagcgtga ttatgaagag aattagtaaa gatatatggg
    19681 cagtatttaa attactgtat caaaataaag ggcgttttag cattaatgcc ttactattgc
    19741 agttaatcat gatttttatt agtagtacat acttaatttt actatttaat atgatgttaa
    19801 aagtagctgg gcaaagccaa cttacgatta acaattggac ggaaatcgtt agtcatcccg
    19861 ccagtgtgat acttcttatt atattcatat taagtgttgc ctttctgatt tatgtagagt
    19921 tttcattgtt agtttatatg gtttatgccg gctttgatcg acagattatt acatttaaat
    19981 ccatttttaa aaatgccttt gtaaatgtgc gtaaactcat aggtgtacca gttattttct
    20041 ttgtcattta tttaatgtta atgataccca ttgccaacct aggactaagt tcagtattaa
    20101 caaaaaatat ttacatacct aaatttttaa cggaagaact tatgaaaacg acgaaaggta
    20161 taa
//
LOCUS       DAXWFK010000034        20108 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.034, whole genome shotgun sequence.
ACCESSION   DAXWFK010000034 DAXWFK010000000
VERSION     DAXWFK010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20108
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.034"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..314)
                     /gene="ecb"
                     /locus_tag="ACGEXS_000952"
     CDS             complement(<1..314)
                     /gene="ecb"
                     /locus_tag="ACGEXS_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement convertase inhibitor Ecb"
                     /protein_id="HFF8011974.1"
                     /translation="MKKNFIGKSILSIAAISLTVSTFAGESHAQTKNVEAAKKYDQYQ
                     TNFKKQVNKKVVDAQKAVNLFKRTRTVATHRKAQRAVNLIHFQHSYEKKKLQRQIDLV
                     LK"
     gene            complement(508..675)
                     /locus_tag="ACGEXS_000953"
     CDS             complement(508..675)
                     /locus_tag="ACGEXS_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011975.1"
                     /translation="MKRIVILENVIVDNPIWLNHVDYLVKSVLLKSIKLRFQEVRKFE
                     ILVKIMNRIHI"
     gene            complement(803..1306)
                     /locus_tag="ACGEXS_000954"
     CDS             complement(803..1306)
                     /locus_tag="ACGEXS_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFF8011976.1"
                     /translation="MSKWIIVSDNHTESGVLYQIYEMHPDADVYLHLGDSEFAYDDTE
                     LSLFNRVKGNCDFYPEFENEAVAKYNDVKAFYTHGHLYQVNRTRDLLAEKGLELGCLF
                     AFYGHTHVAKYEYINGVHVINPGSISQSRSSMEETYAEVIIDDQTLHGTINFKNRHHE
                     TISHTTF"
     gene            complement(1299..1886)
                     /locus_tag="ACGEXS_000955"
     CDS             complement(1299..1886)
                     /locus_tag="ACGEXS_000955"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFF8011977.1"
                     /translation="MKEIVIASNNQGKINDFKVIFPDYHVIGISELIPDFDVEETGST
                     FEENAILKSEAAAKALNKTVIADDSGLEVFALNGEPGIYSARYAGENKSDEANIEKLL
                     NKLGNTTDRRAQFVCVISMSGPDMETKVFKGTVSGEIADGKYGENGFGYDPIFYVPKL
                     DKTMAQLSKEQKGQISHRRNAINLLQAFLEGDKNV"
     gene            complement(1898..2698)
                     /gene="racE"
                     /locus_tag="ACGEXS_000956"
     CDS             complement(1898..2698)
                     /gene="racE"
                     /locus_tag="ACGEXS_000956"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFF8011978.1"
                     /translation="MNKPIGVIDSGVGGLTVAKEIMRQLPNETIYYLGDIGRCPYGPR
                     PGEQVKQYTVEIARKLMEFDIKMLVIACNTATAVALEYLQKTLSIPVIGVIEPGARTA
                     IMTTRNQNVLVLGTEGTIKSEAYRTHIKRINPHVEVHGVACPGFVPLVEQMRYSDPTI
                     TSIVIHQTLKRWRNSESDTVILGCTHYPLLYKPIYDYFGGKKTVISSGLETAREVSAL
                     LTFSNEHASYTEHPDHRFFATGDPTHITNIIKEWLNLSVNVERISVND"
     gene            complement(2934..3749)
                     /gene="sdhB"
                     /locus_tag="ACGEXS_000957"
     CDS             complement(2934..3749)
                     /gene="sdhB"
                     /locus_tag="ACGEXS_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HFF8011979.1"
                     /translation="MTEQSVKNTPQHETQSKPKQKTVKLIIKRQDTSDSKPYEETFEI
                     PYRENLNVIACLMEIRRNPVNIKGEKTTPVVWDMNCLEEVCGACSMVINGRARQSCSA
                     IVDQLEQPIRLEPMNTFPVIRDLQVDRSRMFDNLKRMKAWIPIDGTYDLGPGPRMPEK
                     KRQTAYELSKCMTCGVCLEVCPNVTENNKFVGAQAISQVRLFNLHPTGSMTKDERLNA
                     LMGTGGLQQCGNSQNCVNACPKGIPLTTSIAAMNRETTFHMFKSFFGSDHEVE"
     gene            complement(3749..5515)
                     /gene="sdhA"
                     /locus_tag="ACGEXS_000958"
     CDS             complement(3749..5515)
                     /gene="sdhA"
                     /locus_tag="ACGEXS_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFF8011980.1"
                     /translation="MAEKHLIVVGGGLAGLMSTIKAAEKGAHVDLFSVVPVKRSHSVC
                     AQGGINGAVNTKGEGDSPWIHFDDTVYGGDFLANQPPVKAMTEAAPKIIHLLDRMGVM
                     FNRTNEGLLDFRRFGGTLHHRTAYAGATTGQQLLYALDEQVRAYEVDGLVTKYEGWEF
                     LGIVKGDDDSARGIVAQNMTTAEIETFGSDAVIMATGGPGIIFGKTTNSMINTGSAAS
                     IVYQQGAIYANGEFIQIHPTAIPGDDKLRLMSESARGEGGRIWTYKDGKPWYFLEEKY
                     PDYGNLVPRDIATREIFDVCINQKLGINGENMVYLDLSHKDPHELDVKLGGIIEIYEK
                     FTGDDPRKVPMKIFPAVHYSMGGLYVDYDQMTNIKGLFAAGECDFSQHGGNRLGANSL
                     LSAIYGGTVAGPNAIDYISNIDRSYTDMDESIFEKRKAEEQERFDKLLAMRGTENAYK
                     LHRELGEIMTANVTVVRENEKLLETDKKIVELMKRYEDIDMEDTQTWSNQAVFFTRQL
                     WNMLVLARVITIGAYNRNESRGAHYKPEFPERNDEEWLKTTMASFQGAFEKPQFTYDD
                     VDVSLIPPRKRDYTSKSKGGKK"
     gene            complement(5567..6181)
                     /locus_tag="ACGEXS_000959"
     CDS             complement(5567..6181)
                     /locus_tag="ACGEXS_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b558 subunit"
                     /protein_id="HFF8011981.1"
                     /translation="MAQSKNEFYLRRIHSLLGIIPIGAFLVVHLLVNHQATQGAEAFN
                     KASNFMESLPFLIIVEFLFIYIPLLYHGLFGIHIAFTAKENVGHYSIFRNWMFFFQRV
                     SGILTFIFIGIHLWQTRLQKAFYGKEVNYDLMHETLQHPGWAIFYIICIIAVVFHFAN
                     GLWSFLVTWGGLQSPKSQRVFTWVSLIVFLVISYIGVTAIIAFM"
     gene            complement(6505..8286)
                     /gene="uvrC"
                     /locus_tag="ACGEXS_000960"
     CDS             complement(6505..8286)
                     /gene="uvrC"
                     /locus_tag="ACGEXS_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFF8011982.1"
                     /translation="MEDYKQRIKNKLNVVPMEPGCYLMKDRNDQVIYVGKAKKLRNRL
                     RSYFTGAHDAKTTRLVGEIRRFEFIVTSSETESLLLELNLIKQYQPRYNILLKDDKSY
                     PFIKITKEKYPRLLVTRTVKQGTGKYFGPYPNAYSAQETKKLLDRIYPYRKCDKMPDK
                     LCLYYHIGQCLGPCVYDVDLSKYAQMTKEITDFLNGEDKTILKSLEERMLTASESLDF
                     ERAKEYRDLIQHIQNLTNKQKIMSSDKTIRDVFGYSVDKGWMCIQVFFIRQGNMIKRD
                     TTMIPLQQTEEEEFYTFIGQFYSLNQHILPKEVHVPRNLDKEMIQSVVDTKIVQPARG
                     PKKDMVDLAAHNAKVSLNNKFELISRDESRTIKAIEELGTQMGIQTPIRIEAFDNSNI
                     QGVDPVSAMVTFVDGKPDKKNYRKYKIKTVKGPDDYKSMREVVRRRYSRVLNEGLPLP
                     DLIIVDGGKGHMNGVIDVLQNELGLDIPVAGLQKNDKHQTSELLYGASAEIVPLKKNS
                     QAFYLLHRIQDEVHRFAITFHRQTRQKTGLKSILDDIDGIGNKRKTLLLRSFGSIKKM
                     KEATLEDFKNIGIPENVAKNLHEQLHK"
     gene            8452..8547
                     /locus_tag="ACGEXS_000961"
     CDS             8452..8547
                     /locus_tag="ACGEXS_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8011983.1"
                     /translation="MLDYHLTFIDLLIPPFSSRKQYDDLSFNFLY"
     gene            complement(8610..8924)
                     /gene="trxA"
                     /locus_tag="ACGEXS_000962"
     CDS             complement(8610..8924)
                     /gene="trxA"
                     /locus_tag="ACGEXS_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFF8011984.1"
                     /translation="MAIVKVTDADFDSKVESGVQLVDFWATWCGPCKMIAPVLEELAA
                     DYEGKADILKLDVDENPSTAAKYEVMSIPTLIVFKDGQPVDKVVGFQPKENLAEVLDK
                     HL"
     gene            complement(9097..11445)
                     /locus_tag="ACGEXS_000963"
     CDS             complement(9097..11445)
                     /locus_tag="ACGEXS_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFF8011985.1"
                     /translation="MRQKTLDVLEFEKIKSLVANETISDLGLEKVNQMMPATNFETVV
                     FQMEETDEIAQIYNKHRLPSLSGLSKVSAFIHRADIGGVLNVSELNLIKRLIQVQNQF
                     KTFYNQLVEEDEGVKYPILDDKMNQLPVLTDLFQQINETCDTYDLYDNASYELQGIRS
                     KISSTNQRIRQNLDRIVKSQANQKKLSDAIVTVRNERNVIPVKAEYRQDFNGIVHDQS
                     ASGQTLYIEPSSVVEMNNQISRLRHDEAIEKERILTQLTGYVAADKDALLVAEQVMGQ
                     LDFLIAKARYSRSIKGTKPIFKEDRTVYLPKAYHPLLNRETVVANTIEFMEDIETVII
                     TGPNTGGKTVTLKTLGLIIVMAQSGLLIPTLDGSQLSVFKNVYCDIGDEQSIEQSLST
                     FSSHMTNIVEILKHADKHSLVLFDELGAGTDPSEGAALAMSILDHVRKIGSLVMATTH
                     YPELKAYSYNREGVMNASVEFDVDTLSPTYKLLMGVPGRSNAFDISKKLGLSLNIINK
                     AKTMIGTDEKEINEMIESLERNYKRVETQRLELDRLVKEAEQVHDDLSKQYQQFQNYE
                     KSLIEEAKEKANQKIKAATKEADDIIKDLRQLREQKGADVKEHELIDKKKRLDDHYEA
                     KSIKQNVQKQKYDKIVAGDEVKVLSYGQKGEVLEIVNDEEAIVQMGIIKMKLPIEDLE
                     KKQKEKVKPTKMVTRQNRQTIKTELDLRGYRYEDALIELDQYLDQAVLSNYEQVYIIH
                     GKGTGALQKGVQQHLKKHKSVSDFRGGMPSEGGFGVTVATLK"
     gene            complement(11455..13167)
                     /gene="polX"
                     /locus_tag="ACGEXS_000964"
     CDS             complement(11455..13167)
                     /gene="polX"
                     /locus_tag="ACGEXS_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HFF8011986.1"
                     /translation="MTKKDVIKLLEQIATYMELKGENTFKISAYRKAAQSLELDERPL
                     DEISDVTELKGIGKGVAEVINDYRETGESQYLQQLQEEVPEGLIPLLKIQGLGSKKIA
                     KLYKELNIVDKASLQVACENGKVSELSGFAKKTEQNILEAVKQLGAKKDRYPIDQMRR
                     LNQEIIDYIDTLNYIDQYSSAGSFRRFKEMSKDLDFIISTDNPKAVQQQLLNIPNKVK
                     EVAVGNTKVSLELAYDDETIGVDFRLIEPSAFYHTLQHFTGSKEHNIRIRQLAKARDE
                     KVSEYGIEQADGTLIQYDSEAKIYEHFNVNFIPPAMREDGSEFDKDLSNIITIDDING
                     DIHMHTTYSDGAFSIRDMVEANIAKGYKFMVITDHSQSLRVANGLQVERLLRQNEEIK
                     ALDKEYSEIDIYSGTEMDILPDGSLDYDDEILAQLDYVIGAIHQSFNQSEEQIMERLA
                     NACRNPYVRHIAHPTGRIIGRRDGYKPNIEQLMALAEETNTVLEINANPHRLDLSADI
                     VRKYPNVKLTINTDAHHTNHLDFMNYGVATAQKGFVTKDRVINALSREAFKDFIENNT
                     KLKK"
     gene            complement(13240..13761)
                     /locus_tag="ACGEXS_000965"
     CDS             complement(13240..13761)
                     /locus_tag="ACGEXS_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFF8011987.1"
                     /translation="MVIDFIIIIFFVYFVIVGFRRGFWLSMIHLSATIVSLWIASQFY
                     KSIVERLIVFIPYPKTTAFNTTFAFHFNHLQNRFEAIVAFLMITLFCKFILYLIIVTF
                     DKIIAYQNIHIFSRAMGMIVGVFMTIIVLHFTLYLLALYPNEALQHQLKISIVSHSLI
                     FHIPYLSAFTINL"
     gene            complement(13769..14028)
                     /gene="zapA"
                     /locus_tag="ACGEXS_000966"
                     /pseudo
     CDS             complement(13769..14028)
                     /gene="zapA"
                     /locus_tag="ACGEXS_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499640.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
     gene            14398..15336
                     /gene="rnhC"
                     /locus_tag="ACGEXS_000967"
     CDS             14398..15336
                     /gene="rnhC"
                     /locus_tag="ACGEXS_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFF8011988.1"
                     /translation="MANIVFKLSDKDITTLMSRISFDTENLPQGMKARAKYQNTTVNI
                     YQSGKVMFQGNHAEAVSEELLPQHSQLNTNKTKKKNMANSFLEQTLMYDQFNCIGSDE
                     AGSGDYFGPLTVCAAFVTKEHVPILKTLGVDDSKKLTDTKIVELAEQLVTFIPHSLLT
                     LHNEKYNIQQAKGWTQVKMKAALHNEAIKNVLEKIDSSQLDYIVIDQFAKREVYSHYA
                     LSDIPLPKKTKFETKGESKSLAIAVASIISRYAFVTYMDQISKNINMTIPKGAGAKVD
                     VIAAKIIKKYGLSRLDTISKKHFKNREKAQKILKPL"
     gene            complement(15507..17909)
                     /gene="pheT"
                     /locus_tag="ACGEXS_000968"
     CDS             complement(15507..17909)
                     /gene="pheT"
                     /locus_tag="ACGEXS_000968"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFF8011989.1"
                     /translation="MLISNEWLKEYVTIDDSVSNLAERITRTGIEVDDLIDYTKDIKN
                     LVVGFVKSKEKHPDADKLNVCQVDIGEDEPVQIVCGAPNVDAGQYVIVAKVGGRLPGG
                     IKIKRAKLRGERSEGMICSLQEIGISSNYIPKSFESGIYVFSEAQVPGTDALQALYLD
                     DQVMEFDLTPNRADALSMIGTAYEVAALYNTKMTKPETTSNELDLSANDELTVTIENE
                     DKVPYYSARVVHDVTIEPSPIWMQARLIKAGIRPINNVVDISNYVLLEYGQPLHMFDQ
                     DAIGSQQIVVRQANEGEKMTTLDDTERELLTSDIVITNGQTPIALAGVMGGDFSEVKE
                     QTSNIVIEGAIFDPVSIRHTSRRLNLRSESSSRFEKGIATEFVDEAVDRACYLLQTYA
                     NGKVLKDRVSSGELGAFITPIDITADKINRTIGFDLSQNDIVTIFNQLGFDTEINDDV
                     ITVLVPSRRKDITIKEDLIEEVARIYGYDDIPSTLPVFDKVTSGQLTDRQYKTRMVKE
                     VLEGAGLDQAITYSLVSKEDATAFSMQQRQTIDLLMPMSEAHASLRQSLLPHLIEAAS
                     YNVARKNKDVKLFEIGNVFFANGEGELPDQVEYLSGILTGDYVVNQWQGKKETVDFYL
                     AKGVVDRVSEKLNLEFSYRRADIDGLHPGRTAEILLENKVVGFIGELHPILAADNDLK
                     RTYVFELNFDALMAVSVGYINYQPIPRFPGMSRDIALEVDQNIPAADLLSTIHAHGGN
                     ILKDTLVFDVYRGEHLEKGKKSIAIRLNYLDTEETLTDERVSKVQAEIEAALIEQGAV
                     IR"
     gene            complement(17909..18967)
                     /gene="pheS"
                     /locus_tag="ACGEXS_000969"
     CDS             complement(17909..18967)
                     /gene="pheS"
                     /locus_tag="ACGEXS_000969"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFF8011990.1"
                     /translation="MSEQQTMSELKQQALVDINEANDERALQEVKVKYLGKKGSVSGL
                     MKLMKDLPNEDKPAFGQKVNELRQTIQNELDERQQMLVKEKLNKQLAEETIDVSLPGR
                     HIEIGSKHPLTRTIEEIEDLFLGLGYEIVNGYEVEQDHYNFEMLNLPKSHPARDMQDS
                     FYITDEILLRTHTSPVQARTMESRHGQGPVKIICPGKVYRRDSDDATHSHQFTQIEGL
                     VVDKNVKMSDLKGTLELLAKKLFGADREIRLRPSYFPFTEPSVEVDVSCFKCKGKGCN
                     VCKHTGWIEILGAGMVHPNVLEMAGFDSSEYSGFAFGMGPDRIAMLKYGIEDIRHFYT
                     NDVRFLDQFKAVEDRGDM"
     gene            complement(19348..20088)
                     /locus_tag="ACGEXS_000970"
     CDS             complement(19348..20088)
                     /locus_tag="ACGEXS_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFF8011991.1"
                     /translation="MEQITSAQNNRIKQANKLKKKRERDKTGLALIEGVHLIEEAYQS
                     GIVITQLFAIEPARLDQQIIAYAQEVFEINMKVAESLSGTVTPQGFFAIIEKPHYDIS
                     KAQQVLLIDRVQDPGNLGTLIRTADAAGMDAVIMEKGTTDPYQDKVLRASQGSVFHLP
                     VMTQDLDTFITQFNGPVYGTALENAVAYKEVTSSDSFALLLGNEGEGVNPELLAHTTQ
                     NLIIPIYGKAESLNVAIAGSILLYHLKG"
BASE COUNT         6213 a         3864 c         2811 g         7220 t
ORIGIN      
        1 tattttaaaa ctagatcgat ttgtctttgt aattttttct tttcataact gtgttggaaa
       61 tgaattaaat taacagctct ttgtgcttta cggtgtgttg caacagttct tgtacgtttg
      121 aacaagttta cagctttttg tgcatccaca acttttttat ttacttgttt tttaaagttt
      181 gtttgatact gatcatattt tttagcagct tcaacgtttt tagtttgtgc atgagattca
      241 ccggcaaacg ttgataccgt taaactaata gcggctatgc ttaaaattga tttcccaata
      301 aaattctttt tcataattaa taacacctat cctcaaatat ttattttgta tatatttata
      361 ataatagaca gatacaaaaa actacattaa ctatttatta actaaacatt aatatttgtg
      421 tcactacatt tctgaatttc aagaattttg ctactccata ttttaaccat caaattattg
      481 atataattta tgactttaga aatcctttta aatatgtata cgattcatga ttttaactaa
      541 aatttcaaat ttcctgactt cttggaatct taattttata ctttttaata atacactttt
      601 aaccaaataa tcaacatgat tcaaccatat agggttatca acaatcacat tctccaaaat
      661 tacaattctt ttcataacat aaatgtgtta ttcattccaa ttatcttata aaattgtcag
      721 aaatactttt aaacaaacat tcatgacacg acctcctagt taaatcagtc tataaactaa
      781 aaaaagccat agctacttct atttaaaaag tagtatgact gattgtttcg tgatgtcgat
      841 ttttgaaatt gatggtgcca tgtaaagttt gatcatcaat aataacttca gcatatgttt
      901 cttccattga acttctagat tgagatatac ttccaggatt aataacatga acaccattaa
      961 tatactcata ttttgccaca tgtgtatgtc cataaaatgc aaacaaacaa cctaattcaa
     1021 gtcctttttc agctaataaa tctcttgttc gattgacttg atataaatgt ccatgagtat
     1081 aaaatgcttt cacgtcatta tattttgcga ccgcttcatt ttcaaattct gggtaaaaat
     1141 cacaattgcc ttttactcta ttaaataagc taagttccgt atcatcatac gcgaattctg
     1201 aatctcctaa atgtaaatat acatctgcat ctgggtgcat ttcataaatt tgatataaaa
     1261 cgcctgattc agtatggtta tcactcacaa taatccattt agacattttt atcaccttca
     1321 agaaaagctt gaagtaaatt aatcgcattt cgtctatgac taatttgccc tttttgttct
     1381 tttgaaagtt gagccatggt tttatctaat ttcggtacat aaaatatcgg atcatatccg
     1441 aaaccatttt cgccatattt tccatctgca atttcacctg aaacagtacc tttaaatact
     1501 tttgtttcca tatcagggcc actcatactt atgacacaaa caaattgcgc acgacgatca
     1561 gttgtattac caagcttatt taataatttt tcaatatttg cttcatcgct tttattttca
     1621 ccagcataac gtgcagagta tatacctggc tcaccattta atgcaaaaac ttctagtcca
     1681 ctgtcatcag ctatgaccgt tttattcaat gcttttgcag cagcttctga ttttaatata
     1741 gcattttctt caaatgttga tcctgtttct tccacatcaa aatctggtat tagttctgaa
     1801 atacctatta cgtggtaatc tggaaatatt actttaaagt catttatttt cccttgatta
     1861 ttcgatgcaa taacaatctc tttcattaaa aatcctccta gtcattcact gatatacgtt
     1921 ccacattgac agataaattt aaccactctt tgataatgtt agtaatgtga gtaggatcac
     1981 ctgttgcaaa aaatcgatga tctggatgtt cagtataact tgcatgttca ttactaaatg
     2041 ttagcaatgc actaacttca cgagccgttt ctaatccaga cgaaatcact gtctttttac
     2101 caccaaaata atcatagata ggtttataga gcaatggata gtgggtacat cctaaaatga
     2161 cagtatcaga ctcactatta cgccaacgtt tcagtgtttg atgaatgaca atgcttgtaa
     2221 ttgttggatc actatatctc atttgttcta caagtggcac aaaacctgga caggcaacgc
     2281 catgtacctc tacatgtgga ttgatacgtt taatatgcgt acgatatgct tcagatttaa
     2341 ttgtgccttc cgttcctagt actaatacat tttgatttct agtagtcatt attgctgttc
     2401 tagcacctgg ttcaattacg ccaatcactg ggattgataa ggtcttttgt aaatattcta
     2461 aagctacagc agttgctgta ttacaagcaa tcacgagcat ttttatatca aattccatta
     2521 atttacgagc gatttcaact gtatattgtt ttacttgttc tcctggtctt ggcccatatg
     2581 gacatcgtcc aatatcacct aagtaataaa tcgtctcatt tggcaactga cgcataattt
     2641 ctttagctac tgtcaaacct ccgacaccag agtctattac acctattggt ttattcatat
     2701 tcggtcatcc ttacttttaa tttaattttt agtttatcat aactaagcat tggattttag
     2761 tattatgcac tgtgtttacc atttttgtca ttataatatt tattttaaat cagcccacta
     2821 tcatattgtc atgtaatctg cttattaaaa aaatcccttc caagttattg tgtatctcca
     2881 ttcaatttaa ttttgaaagg aacataacat tttaactcaa aagggattaa tttttattct
     2941 acttcatggt ctgaaccaaa gaatgattta aacatgtgga atgttgtttc tctgttcatt
     3001 gctgcaatgg atgttgttaa tggaatacct ttagggcaag cattaacaca gttttgtgaa
     3061 ttaccacact gctgtaagcc accagtaccc attaatgcat ttaaacgttc atctttagtc
     3121 atagatcctg ttgggtgcaa attaaacaaa cgaacttgcg agattgcttg tgcaccaacg
     3181 aatttattat tttcagtaac attaggacaa acctctaaac atacaccaca tgtcatacat
     3241 ttagataatt cataagctgt ttgacgtttt ttctctggca tacgtggtcc cggacctaaa
     3301 tcatacgttc catcaattgg gatccatgct ttcatacgtt ttaagttatc gaacattcta
     3361 gaacgatcaa cttgtaagtc acggataact gggaaagtat tcattggctc taaacgaata
     3421 ggttgttcta attgatcaac aatcgcagaa caagattgtc ttgcacgacc attgataacc
     3481 atagaacatg ctccacatac ttcttctaag cagttcatat cccagacaac aggtgttgtt
     3541 ttttcacctt taatattaac tgggttacgt ctaatttcca ttaaacaagc aatgacgttt
     3601 aaattttcac gatatggaat ttcaaatgtt tcttcataag gcttagaatc acttgtatct
     3661 tgtcgtttaa taattaattt tactgttttt tgtttcggtt tagattgtgt ttcatgttgt
     3721 ggagtgtttt tcactgattg ttcagtcatt attttttacc ccctttagac ttacttgtgt
     3781 aatcacgttt acgaggtggt attaaactca catcgacgtc atcataagta aactgcggtt
     3841 tttcaaatgc gccttggaat gaggccattg tcgtttttaa ccactcttca tcattacgct
     3901 ctgggaattc tggtttataa tgggcaccgc gcgattcgtt acggttatat gcaccaatcg
     3961 taataacacg tgcaagtact aacatgttcc atagttgacg ggtaaagaat accgcttggt
     4021 tactccaagt ttgagtatct tccatatcaa tatcttcata acgtttcatc aattcaacaa
     4081 tctttttatc tgtttctaac agtttttcat tttcacgaac aacagttaca tttgctgtca
     4141 taatttcacc aagttcacgg tgtaatttat atgcattttc tgtaccgcgc atagctaata
     4201 atttatcaaa acgttcttgc tcttcagctt tacgcttttc aaaaatactt tcgtccatat
     4261 cagtatatga tcgatcaata tttgaaatat aatcaatcgc gtttggacct gctactgtac
     4321 caccataaat cgctgataac aatgaattgg cacctaagcg gttaccacca tgttgagaga
     4381 agtcacattc tccagctgca aataaccctt taatatttgt catttgatca taatctacat
     4441 atagaccacc cattgaatag tgaacagctg ggaaaatctt cattggtact ttgcgtgggt
     4501 catcaccagt gaatttttca taaatctcaa tgataccacc tagttttaca tctaactcat
     4561 gtggatcttt atgtgacaaa tcaagatata ccatgttttc gccatttata cctaattttt
     4621 ggttaataca tacatcgaaa atttcacgcg ttgcgatatc acgaggtact aagttaccat
     4681 aatcaggata tttctcttct aagaagtacc aaggcttacc atctttatat gtccaaattc
     4741 gtccaccttc accacgtgct gattcactca ttagtcgcag tttatcatca ccagggattg
     4801 cagtaggatg aatttgaatg aactcaccat tagcataaat agcgccttgt tggtaaacaa
     4861 tggaagccgc tgatcctgta ttaatcattg agtttgttgt tttaccgaaa ataataccag
     4921 ggccacccgt tgccataata actgcatctg aaccaaatgt ttcaatctca gcagttgtca
     4981 tattttgtgc aacgatacct cttgcactat catcgtcacc tttaactatg ccaaggaatt
     5041 cccatccttc atacttcgta actaatccat ctacttcata tgcacgaact tgttcatcca
     5101 atgcatataa taattgttgt ccagttgttg cccctgcata tgctgttctg tgatgtaatg
     5161 taccaccgaa acgtctaaaa tctaatagac cttcatttgt tctattgaac attacgccca
     5221 tacggtctaa taaatgaata attttaggtg ctgcctctgt catcgcttta acaggtggtt
     5281 ggtttgcaag gaaatcgcca ccatacactg tatcatcaaa gtgaatccaa ggagaatcgc
     5341 cttccccttt agtattgacc gcaccattaa tgccaccttg ggcacaaaca gagtgcgaac
     5401 gctttactgg tacaactgag aacaaatcta catgtgcacc tttttctgcc gctttaattg
     5461 ttgacattaa gcccgctagg ccacctccga caacaataag atgtttctct gccataaaaa
     5521 tttcactccc ctaaattttc aatctatatt tgttaaatgc gatgtattac ataaaggcaa
     5581 taattgcagt aacaccaata tacgaaataa ctaaaaatac gattaatgaa acccatgtaa
     5641 atactcgttg tgattttgga gattgaagtc caccccaagt aactaagaat gaccataagc
     5701 catttgcaaa gtggaacaca acagcaataa tacaaataat ataaaatatt gcccatccag
     5761 gatgttgcaa tgtttcgtgc attaaatcgt aattcacttc tttgccgtaa aatgcttttt
     5821 gtaaacgtgt ttgccataaa tggataccaa taaagataaa tgttaagata ccactcactc
     5881 tttggaagaa gaacatccag tttctaaaaa tcgagtaatg tccaacattt tcttttgctg
     5941 taaatgcaat gtgtatacca aacaaaccgt gatataacaa cggaatgtat ataaataaaa
     6001 attctacaat aattagaaat ggtaatgatt ccataaagtt agatgcctta ttaaacgctt
     6061 cagcaccttg tgttgcttgg tgattcacta ataaatgaac gaccaaaaat gcacctattg
     6121 ggataatacc taataacgag tgaatacgtc ttagataaaa ttcatttttt gattgagcca
     6181 aaaggagtcc cccctgtgaa cgaatattta atttattgag ctatttatat taaacgtacg
     6241 cttaaccccc taaagtgata tcgtttctag cgttgtcatt atcattaagc gaaacatttt
     6301 aaagacaaat acacactgca cgatcaccaa actgcatgtc gaacaatgta acatttggat
     6361 tcgatattta aaattgcttg tgatgataaa ctttctcatt tagaaaacgc ttccacgtac
     6421 attcaaaaaa ataactttgt taaccatatt gtaacattat ttcatatatt ttggggcatg
     6481 agaatgattc tcacgcccag taatttattt atgcaattgt tcatgtaggt tctttgcgac
     6541 gttttcagga atacctatat ttttaaaatc ttcaagtgta gcttccttca ttttcttgat
     6601 tgaaccgaat gaacgcaata ataatgtttt acgtttgtta ccgataccat ctatatcatc
     6661 aagtattgat ttcaagcctg tcttttgacg tgtttgtcta tgaaatgtga ttgcgaatct
     6721 gtgaacctca tcttggatac ggtgcaacaa ataaaatgcc tggctatttt tcttcagtgg
     6781 tacaatttct gcactagcgc catataataa ttcagatgtt tggtgtttat catttttctg
     6841 caaacctgca acagggatat caagacctaa ttcgttttgt agcacatcaa taaccccgtt
     6901 catatgtcct ttaccaccat ctactattat taaatcaggt aatggtaatc cttcgtttaa
     6961 aacgcgagaa tatcgtcgtc ttactacttc tctcattgat ttgtaatcat ctggaccttt
     7021 aaccgttttg attttatact ttctataatt tttcttatct ggtttaccgt cgacaaatgt
     7081 aaccattgct gacactggat ccacaccttg aatattagaa ttatcgaatg cttcaattct
     7141 aattggtgtt tgaattccca tttgtgttcc aagttcttca atagctttaa tcgttctgga
     7201 ctcatcacgt gatattaatt caaatttatt atttaaggat actttagcgt tatgtgcagc
     7261 taggtcaacc atatcttttt tgggacctcg cgcgggttga acgattttag tgtccacaac
     7321 agattgaatc atttctttat ccaaattacg tggtacatga acttccttag gtaaaatatg
     7381 ttggtttaag ctataaaatt gtccaataaa tgtataaaat tcttcttctt ctgtttgctg
     7441 taatggaatc atcgttgtat ctcgctttat catattacct tgtcgtataa agaaaacttg
     7501 gatacacatc catcctttat caacactata accaaagaca tcacgaatcg ttttatctga
     7561 tgacataatt ttttgtttgt ttgtcagatt ttgaatatgt tgaattaaat ctctatattc
     7621 tttagcccgt tcaaaatcaa gtgattcact tgcagttaac attcgctctt ctaaactttt
     7681 taaaattgtt ttgtcttccc cattcagaaa atcagtaatt tccttcgtca tttgtgcgta
     7741 tttactcaaa tcaacgtcat atacacatgg tcctaaacat tgtccaatat ggtaataaag
     7801 acataattta tctggcatct tatcacattt gcgatatgga tatattctgt ctaataactt
     7861 tttagtttct tgagcagaat atgcattcgg atacggtccg aaatatttgc cagtaccttg
     7921 ttttacagtt ctcgtcacta gtagtctagg atatttctcc ttcgtaattt taataaatgg
     7981 ataactttta tcatccttta ataatatatt atatcttggt tgatattgtt taatcagatt
     8041 caattccagt aaaagtgatt ctgtttcact tgacgtgaca ataaactcaa agcgacgtat
     8101 ttcaccaacc agtctcgttg ttttagcatc atgagcaccc gtaaaatatg atcgcaatcg
     8161 atttcttagc tttttagctt tgccaacata tatcacttga tcattacgat ctttcattaa
     8221 atagcatcct ggttccatag gtacgacatt taatttattt ttaattcgtt gcttatagtc
     8281 ttccaaaaat atcccccctt ctaattttaa aatgaatgat gctacaaaag tgcttcaaat
     8341 ttatcaccac taattctagt taataatcaa catcttcgag tatgactttt catgcacatc
     8401 atgtaatatt gaacatggtt ttatcaaacc aactgctaca aatctatcag catgctagat
     8461 tatcatttaa cgttcataga tttattaatc ccaccattct caagtcgtaa acaatatgat
     8521 gacttatcat tcaacttttt atattaaaaa cacaaacaaa aactggagca attcattaaa
     8581 gaaatgcccc agtcgtcatt ggttgtaact tataaatgtt tatctaaaac ttcagctaag
     8641 ttttcttttg gttggaaacc aacaacttta tcaactggtt gaccgtcttt aaagacgatt
     8701 aatgttggaa tactcatcac ttcatattta gctgcagttg atggattttc atcaacatct
     8761 aattttaaaa tgtcagcttt accttcatag tcagctgcta attcttctaa taccggagcg
     8821 atcattttac atggaccaca ccatgttgcc caaaaatcta ctagttgtac accagattct
     8881 acttttgaat caaaatctgc atctgttact tttacgattg ccataaatgc caatcctcct
     8941 taattattat tatgatgttc aaattatatc agataacatg cttcatgtat ttctatttgc
     9001 tcaattttta tgcttatttc aaataacatg tttgaaatgt attcttgttg ctttacatta
     9061 ttttaactgc agctatttaa tttatcaaat tataatttat tttagtgttg caacggtaac
     9121 gccaaatcca ccttcgcttg gcataccacc tctaaagtca ctaacacttt tatgcttttt
     9181 caaatgttgt tgtacacctt tttgaagtgc acctgtacct ttaccatgaa tgatataaac
     9241 ttgttcgtaa ttacttaaaa cggcttgatc taaatattga tctagttcaa ttaaagcatc
     9301 ctcataacga tagcctcgta agtcaagttc agttttaatt gtttgacgat tttgacgtgt
     9361 aaccattttc gttggcttaa ctttttcttt ttgttttttc tctaaatctt caataggtaa
     9421 cttcatttta ataattccca tttgaacaat tgcttcttca tcattgacaa tttctaaaac
     9481 ttcacccttt tgaccgtaag ataatacttt tacttcatca ccagcaacaa ttttatcgta
     9541 tttttgcttt tgtacatttt gctttataga tttcgcttca taatgatcat ctaatcgttt
     9601 cttcttatca atcaattcat gttctttaac atctgcacct ttttgttcac gcaattgtct
     9661 taagtcttta ataatatcgt cagcttcttt tgttgcagcc ttaatcttct gatttgcttt
     9721 ttctttcgct tcctctatta gagacttttc ataattttgg aattgttgat actgcttaga
     9781 taaatcatca tgcacttgct ccgcttcttt tacaagacgg tccagttcta acctctgtgt
     9841 ctctacacgt ttgtaattac gctctaatga ttcaatcatt tcatttattt ctttttcatc
     9901 agtaccaatc atcgtcttag ccttattaat aatattcaaa ctaagaccta actttttaga
     9961 aatgtcaaaa gcatttgaac gacccggcac acccattaat aacttatacg ttggactcaa
    10021 agtatctaca tcaaattcta cactcgcatt cataacgcct tctcgattat aactatatgc
    10081 tttaagttca ggatagtgcg tcgttgccat tactagagaa ccaatttttc taacatgatc
    10141 taaaatgctc attgctaatg cagcaccttc actcggatct gtacctgcac ctaattcatc
    10201 aaataaaact aaactatgtt tgtctgcatg ctttaaaatt tcaactatat tcgtcatatg
    10261 agatgaaaaa gttgataatg attgttctat tgattgttca tctccgatat cgcaatatac
    10321 atttttaaat acacttaact gactaccatc aagtgtggga atcaacaatc ctgattgagc
    10381 cataacaata attaaaccta atgtttttaa tgttacagtt ttaccacctg tattcggtcc
    10441 tgtaataatt accgtttcaa tatcttccat aaattcgatg gtattagcta caacagtctc
    10501 acgatttaat aatggatggt atgctttagg taaatataca gtacggtcct ctttaaatat
    10561 cggctttgtt cctttaatac ttctactata tctcgctttt gcgattaaaa aatctaactg
    10621 acccatgact tgttctgcca caagtagtgc atctttgtcc gcagccacat aaccagttag
    10681 ttgcgttaaa atgcgttctt tttcaattgc ttcgtcatga cgtaatcgac taatttgatt
    10741 attcatttca acaactgatg atggctcaat atacaatgtt tgtcctgaag cagattgatc
    10801 atgtacaatc ccattaaaat cttgtcgata ttcagctttg acaggtataa cgtttctttc
    10861 attcctaact gttacaatag catctgataa ttttttctga tttgcttggc ttttaacaat
    10921 acggtccaaa ttttgtctaa tacgttgatt cgtgctagaa attttacttc taatcccttg
    10981 caattcataa ctcgcattat catataaatc atacgtatcg catgtttcat ttatttgttg
    11041 aaaaagatca gttaacacag gtaattgatt catcttgtca tctaatattg ggtatttaac
    11101 accttcatct tcttcaacca attgattata aaatgtcttg aattgatttt gtacttgaat
    11161 taatcttttt atcaagttaa gctctgatac atttaaaacg ccgccaatat cagcgcgatg
    11221 aatgaatgct gatactttag ataagccact caagcttggt aaacgatgct tattatagat
    11281 ttgagcaatc tcatccgttt cttccatttg aaaaacaacc gtttcaaaat tagtagctgg
    11341 catcatttga ttgacctttt ccaagcctaa gtcactaata gtttcattgg caacgagtga
    11401 ttttattttt tcaaattcta agacgtctaa tgttttttgt ctcataaaat ccctctattt
    11461 cttaagtttt gtattatttt caataaagtc tttaaaagct tcacgcgata atgcgttaat
    11521 cactctatct tttgttacaa atcctttttg cgcagttgct acgccataat tcataaaatc
    11581 taaatgattt gtatgatgcg catcagtgtt aatagttaat ttcacatttg gatatttacg
    11641 aacgatatca gcgctcagat ccagtcgatg tggattggca ttaatttcta atactgtatt
    11701 cgtttcttca gctaatgcca ttaattgttc aatattcggt ttataaccat ctcttctacc
    11761 tataatacgc cctgttggat gcgctatatg tcgcacgtat ggattgcgac atgcattagc
    11821 taatcgttcc ataatttgtt cttctgattg gttaaagctt tgatgaatag ctccaattac
    11881 ataatcaagt tgtgctaaaa tttcatcatc ataatccagc gagccatcag gtaatatatc
    11941 catttctgta cctgaataaa tatcaatttc actatattct ttatctaaag ccttaatttc
    12001 ttcgttttgt cttaaaagtc tttctacttg taagccatta gcaacacgta aactttgtga
    12061 atgatcagta attaccatga atttataacc ttttgcgata tttgcttcta ccatgtctcg
    12121 aatagaaaac gcaccatcac tatacgttgt atgcatatga atatcaccat taatatcatc
    12181 tattgtaatg atattactta gatctttatc aaattcgcta ccatcttctc gcatagcagg
    12241 tggtataaaa ttcacattaa aatgttcata tatcttggct tcactatcat attgaattaa
    12301 tgtaccatca gcttgttcaa ttccatattc acttactttt tcatcacgtg ctttagcaag
    12361 ttgtcgaatt cttatattat gttcttttga cccagtaaaa tgctgcaatg tatgataaaa
    12421 agcacttggt tcaattaatc gaaaatcgac accaatcgtt tcatcatcat acgctaattc
    12481 taatgaaact tttgtgttcc ccactgcaac ttcttttact ttattgggaa tatttaataa
    12541 ttgctgctgc actgcttttg ggttatcggt acttattatg aaatctaaat ctttgctcat
    12601 ttctttaaaa cgacggaagc ttcctgcaga tgaatattga tcgatataat ttaatgtatc
    12661 tatataatca atgatttctt gattaagtct tctcatttga tcaattggat atctatcttt
    12721 cttagcacca agttgtttca cagcttctaa tatgttttgt tccgttttct tagcaaatcc
    12781 gcttaattca ctaacttttc cattttcaca agcaacttga agtgacgctt tatcaacaat
    12841 attcaactct ttatatagct tagcaatttt cttgcttcca agtccttgaa ttttcaaaag
    12901 tggaataaga ccttccggaa cttcttcctg taattgctgt aaatactgag attcaccggt
    12961 ctcacggtaa tcattgatta cttctgcaac acctttacca atgcctttta actccgttac
    13021 atcagatatt tcatctaatg gtcgttcatc taattcaaga ctttgagctg cttttcgata
    13081 cgctgatatt ttaaaagtat tttccccttt taattccata taagtagcaa tttgttctaa
    13141 tagtttgata acatcttttt ttgtcataat aacactccat aaaaagaaga ccaggacgta
    13201 tcattaatat atacctttgt cctgacctct tatgataatt tataaattaa tggtgaaagc
    13261 cgataaatat gggatgtgaa aaatcaatga atgactcaca atagatattt taagctgatg
    13321 ttgtaatgct tcgttaggat ataatgccaa tagatataac gtaaagtgta agacaattat
    13381 cgtcataaac acaccaacta tcattcccat tgcacgactg aaaatatgaa tgttttgata
    13441 cgctattatt ttatcaaaag ttacgataat tagatataaa atgaacttac aaaacaatgt
    13501 aatcattaaa aaagctacaa tcgcttcaaa tcgattttgt agatgattaa aatgaaacgc
    13561 aaaagttgta ttaaatgctg ttgttttagg atatggaata aatacaatta atctttctac
    13621 aatagatttg taaaattgac tggcaatcca caatgataca atcgttgcac tcaaatgtat
    13681 catagataac caaaaacctc gtctgaatcc aacgatgaca aaatacacaa agaaaatgat
    13741 tatgataaaa tcaatgacca tttattgctc acgctgctgc aatttgtgaa tttgttgttt
    13801 caaacgtcga ttttcttctt ctagtagtac tttttcatgc ataatattca cagcagttag
    13861 tattgctttt cttgaagtat ctaaacctgc tgctttatac cctaattctt ttattttatc
    13921 atcaactaaa tgtgctacat atcgtatgtg ctctgggtta tcttccccaa caattgtaaa
    13981 aagctgatca ttaattgata catttacctt gtttttaaac tgtgtcattt ataatttctc
    14041 ctgatccttt ttttaaaatc taaattcacg ttataaaata tgactggata gtttgtctga
    14101 atttgatact aatattgtta tattgcaatt atgataaaac aacaacacaa tctctataga
    14161 tgacttaatg ttctttttat aatgaaataa tgtaaagaat tttctattca atactttatc
    14221 atgtttaaat tgtgtcacta taacattttc ataaacatta tacatgacca ctatgtattt
    14281 tgtaagtatc cgcaattaat tctttacaac atacataaat gtttctgacg ttattatcat
    14341 ttatgatatg attatttttg ctaaagacaa tgaaatttta tgaaaggatt tacacaaatg
    14401 gcgaatatcg tttttaaatt gtcggataaa gacataacga cattaatgtc acgcatttct
    14461 tttgatactg agaatttacc tcaaggtatg aaagcacgtg caaagtatca aaatacaact
    14521 gtaaatattt accaatcagg taaagtcatg tttcaaggta atcatgcaga agctgtgtct
    14581 gaagaacttc taccgcagca ttctcaattg aatactaata aaactaaaaa gaaaaatatg
    14641 gctaattctt ttcttgaaca aactttaatg tatgatcaat ttaactgtat tggtagtgat
    14701 gaggctggaa gtggcgatta ctttggtccg ttgactgtgt gtgctgcttt cgttaccaaa
    14761 gagcatgtcc ctattttaaa aactttgggt gtagatgatt caaaaaaatt aaccgatacc
    14821 aaaattgtag aacttgctga gcaattggtt actttcatac cacattcatt attaacacta
    14881 cacaatgaaa aatataatat tcagcaagca aaaggctgga ctcaagtaaa aatgaaagct
    14941 gcccttcata atgaagccat caaaaatgta cttgaaaaaa ttgattcgtc tcaattagac
    15001 tatatcgtca ttgatcagtt cgcaaaacgc gaagtgtata gccattacgc attatcagat
    15061 ataccacttc caaagaaaac aaaatttgaa acaaaaggag aatcgaagtc attagccatt
    15121 gcagtggcaa gcataatttc tcgatacgca tttgttacat atatggatca aatatcaaaa
    15181 aatattaata tgacgattcc taaaggtgcc ggtgctaaag tcgatgtaat cgccgccaaa
    15241 attataaaaa aatacggtct ttcacgttta gataccattt caaaaaagca ttttaaaaat
    15301 cgtgaaaaag cccaaaaaat tttaaagcct ctttgatttt taatcattag aggcttttat
    15361 gcagttgtgc gaagcatcat ctgtattccg ctgttcctct ttgacttttt aaatgcattg
    15421 gaaccaaatt ggtgaaccca aaaataaaac cccatgttag ggatatcaat ctacttcaga
    15481 tatccttata catggggttt aaatcattat ctaataacag caccttgttc aattaatgct
    15541 gcttcaattt ccgcttgtac ttttgaaacg cgctcatctg tcaatgtttc ttctgtgtct
    15601 aaataattta aacgtattgc aattgatttt ttaccttttt ctaaatgttc gccccgatat
    15661 acatcaaaga caagtgtatc ttttaatata ttgccaccat gtgcatgaat cgttgataat
    15721 aaatcagctg ctggaatatt ttgatctact tctaatgcaa tgtcacgaga catgcctggg
    15781 aatctcggaa ttggctggta attaatgtaa cctaccgaca cagccattaa tgcatcaaaa
    15841 ttcaactcaa aaacatacgt acgttttaaa tcattatcag ctgctaatat tggatgtaat
    15901 tcaccaataa aaccaacaac tttattctct aataagattt cagcagtacg acctggatgt
    15961 aatccatcaa tatcagcacg gcgataacta aattcaagat ttaacttttc agatactcga
    16021 tccacgacac cttttgctaa atagaaatca accgtttctt tcttaccttg ccattgattg
    16081 actacataat ctccagttaa aataccactt aaatattcaa cttgatctgg tagttcacct
    16141 tctccattag caaagaagac attgccgatt tcaaataatt ttacatcttt atttttgcgt
    16201 gccacattat atgatgccgc ttcgattaaa tgtggtaata aactttgacg taatgacgca
    16261 tgcgcttcac tcattggcat caataaatca attgtttgac gctgttgcat cgaaaatgca
    16321 gtagcatctt ctttagaaac taacgaatac gtaatagctt ggtctaatcc agcaccttct
    16381 aacacttctt taaccattct agttttatat tggcgatcag ttagctgacc actagtaact
    16441 ttatcgaaga caggtaacgt tgatggaata tcgtcgtatc catatatacg tgcaacttct
    16501 tcaattaaat cttctttaat tgtaatatct ttacgacgtg atggtactag cactgtaata
    16561 acatcatcat ttatttctgt atcaaaccct agttgattaa aaatagtaac aatatcattt
    16621 tgtgacaaat caaatccaat agtgcgatta attttatcag cagtgatgtc gattggtgta
    16681 ataaatgcac caagttctcc tgaagacact ctatctttta gcacttttcc gtttgcataa
    16741 gtttgtaata aataacatgc acggtcgact gcttcatcta caaattcagt agctattcct
    16801 ttttcaaaac gactagatga ttcactgcgt aaatttaaac gtcttgatgt atgacgaatt
    16861 gaaactggat caaaaatagc accttcaatc actatatttg atgtttgttc tttaacttct
    16921 gaaaaatcgc cacccataac accagctaat gcaattggag tttgtccatt agtaatgaca
    16981 atatcgctcg ttaataattc acgttctgta tcatctaatg ttgtcatttt ttcgccttca
    17041 ttagcttgac gaacaacaat ttgttgtgaa ccaatcgcat cttgatcaaa catgtgcaat
    17101 ggttgaccgt attctaataa cacataattt gaaatgtcaa caacattatt aataggacgt
    17161 atacccgctt ttattaagcg tgcttgcatc caaattggcg agggttcaat tgtcacgtcg
    17221 tgaacaacac gtgcactata atatggtact ttatcttcat tttcaattgt cacagtcagt
    17281 tcatcatttg cagataaatc aagctcattt gatgttgtct ctggcttagt catttttgta
    17341 ttatataatg ctgcaacttc ataagcagta cctatcatac ttaaagcatc tgcacgattc
    17401 ggcgttaaat caaattccat tacttgatca tctaaatata aagcttgtaa ggcatctgtt
    17461 cctggaactt gggcttcact aaatacataa atgcctgatt caaaactttt cggtatatag
    17521 ttacttgaaa taccaatttc ttgtaacgaa caaatcatac cttctgaacg ttcaccgcgt
    17581 aatttggcac gcttaatttt aataccacca ggcaatctgc cacctacttt agcaacaatg
    17641 acatattgtc ctgcatcaac gttcggtgca ccacaaacga tttgtacagg ttcgtcttct
    17701 ccgatatcaa cttggcaaac atttaattta tcagcatcag gatgtttctc ttttgactta
    17761 acgaagccga caactaaatt tttgatatct tttgtgtagt caattaaatc atccacttca
    17821 atacctgtgc gcgtaatacg ttctgccaaa ttacttacag aatcatcgat tgttacatat
    17881 tctttcaacc attcatttga tatcaacatt acatgtcacc tctatcttct accgctttaa
    17941 attgatctaa aaatctcaca tcattagtat agaaatgacg aatatcttct ataccatatt
    18001 tcaacattgc aatacggtct ggtcccatac caaatgcaaa tccagagtac tctgaagaat
    18061 caaaaccagc catttctaat acattaggat gtaccattcc agcacctaaa atttcaatcc
    18121 atcctgtgtg tttacacaca ttacaacctt ttcctttaca tttaaaacat gacacatcaa
    18181 cttctacaga aggttcagtg aatgggaagt aacttggacg taaacgaatt tcacgatcag
    18241 caccaaataa tttcttagct aacaattcta aagtaccttt caaatcactc attttaacgt
    18301 ttttatcaac aactaatcct tcgatttgtg taaattgatg actatgtgtc gcatcatcag
    18361 agtcacgacg atacacttta ccagggcaaa taattttaac tggaccttga ccatgacgtg
    18421 attccatcgt acgtgcctgc actggtgatg tatgcgtacg taataaaatt tcatccgtaa
    18481 tatagaaact atcttgcata tcacgtgcag ggtgtgattt aggtaaattc agcatttcga
    18541 agttataatg atcttgttca acttcatatc cattcacaat ttcataacct aaacctaaga
    18601 ataagtcttc aatttcttct attgtacgtg ttaatggatg ctttgaaccg atttcaatat
    18661 gacgacctgg taatgataca tcaattgttt cttcagccaa ttgcttattt aatttttctt
    18721 taactaacat ctgttgtctt tcatctaatt cattttgaat tgtttgacgc aattcattca
    18781 ctttttgacc aaacgcaggt ttatcttcat tcggcaaatc cttcatcaat ttcattagtc
    18841 cgctaactga ccctttttta cctaagtatt tcactttaac ttcttgcagt gcacgttcat
    18901 catttgcttc attaatatct acaagcgctt gttgttttaa ctctgacatt gtttgttgtt
    18961 cagacatatg tcactgacct ccctattcga actattcatt ttctatttat catatttgtt
    19021 gtttaactaa aaaaagactt tcatcctctt aaaaaataag ggacgaaagt catttccgtg
    19081 gtaccaccca aatttatgcc taagcataca cttgtttaat attgataacg gattgtcttt
    19141 ccgacttaaa tctctttaag taaccaaaaa ggtgaaaaga ctttaaataa ttggaataac
    19201 ttgcagtcac gattattctc cctaaaacaa ttatttatat aaatgtccat gcctttttat
    19261 tggtttatat tcaattgtgt atgcattcac gtagaaatgt tctgttaaac aattaattat
    19321 aattatatcg gaaaactttc aacacggtca acctttcaaa tgataaagta aaatactacc
    19381 tgcaatcgct acatttaaac tttcagcttt accataaata ggtatgatta aattttgtgt
    19441 agtatgtgct aataattcag gattaacacc ttctccctca ttacctaata gtaatgcaaa
    19501 agaatcactt gaagtaactt ctttgtatgc cactgcgttt tcaagtgctg taccataaac
    19561 aggaccatta aattgagtaa taaacgtatc gagatcttgt gtcataactg gcaaatggaa
    19621 aacactacct tgactcgctc gcaacacttt atcttgataa ggatctgtcg tacccttctc
    19681 cattattaca gcatccattc cagcagcatc cgcagttcta attaatgtgc ctaaatttcc
    19741 aggatcttga acacgatcga tgagcaatac ttgttgtgct ttagaaatat cataatgcgg
    19801 cttctcaatg attgcgaaaa acccttgtgg tgtcactgta cctgataaag attcagcaac
    19861 tttcatgttt atttcaaaaa cttcttgcgc gtatgcgata atttgctgat ctaatcttgc
    19921 cggttcaatt gcaaataatt gtgtaattac aattccactt tgataagctt cttcaattaa
    19981 atgcacacct tcaattaaag ctaatccagt tttatccctc tcacgtttct tttttagctt
    20041 gttcgcttgt ttaattctat tattttgtgc agaagtaatt tgttccattg atagctcctc
    20101 gctttatt
//
LOCUS       DAXWFK010000035        20082 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.035, whole genome shotgun sequence.
ACCESSION   DAXWFK010000035 DAXWFK010000000
VERSION     DAXWFK010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20082
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.035"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..2503
                     /gene="mprF"
                     /locus_tag="ACGEXS_000971"
     CDS             <1..2503
                     /gene="mprF"
                     /locus_tag="ACGEXS_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFF8011992.1"
                     /translation="KIFSILKITFATALFIFVAITLYRELSGINFKDTLVEFSKINRM
                     SLVLLFIGGGASLVILSMYDVILSRALKMDISLGKVLRVSYIINALNAIVGFGGFIGA
                     GVRAMVYKNYTHDKKKLVHFISLILISMLTGLSLLSLLIVFHVFDASLILDKITWVRW
                     VLYVVSFFLPLFIIYSMVRPPDKNNRFVGLYCTLVSCVEWLAAAVVLYFCGVIVDAHV
                     SFMSFIAIFIIAALSGLVSFIPGGFGAFDLVVLLGFKTLGVPEEKVLLMLLLYRFAYY
                     FVPVIIALILSSFEFGTSAKKYIEGSKYFIPAKDVTSFLMSYQKDIIAKIPSLSLAIL
                     VFFTSMIFFVNNLTIVYDALYDGNHLTYYILLAIHTSACLLLLLNVVGIYKQSRRAII
                     FAMISILLITVATFFTYASYILITWLAIIFVLLIVAFRRARRLKRPVRMRNIVAMLLF
                     SLFILYVNHIFIAGTLYALDIYTIEMHTSVLRYYFWLTILIIAIIIGMIAWLFDYQFS
                     KVRISSKIEDCEEIINQYGGNYLSHLIYSGDKQFFTNENKTAFLMYRYKASSLVVLGD
                     PLGDENAFDELLEAFYNYAEYLGYDVIFYQVTDQHMPLYHNFGNQFFKLGEEAIIDLT
                     QFSTSGKKRRGFRATLNKFDELNISFEIIEPPFSTEFINELQHVSDLWLDNRQEMHFS
                     VGEFNEEYLSKAPIGVMRNEENEVIAFCSLMPTYFNDAISVDLIRWLPELDLPLMDGL
                     YLHMLLWSKEQGYTKFNMGMATLSNVGQLHYSYLRERLAGRVFEHFNGLYRFQGLRRY
                     KSKYNPNWEPRFLVYRKDNSLWESLSKVMRVIRHK"
     gene            complement(2706..3215)
                     /gene="msrA"
                     /locus_tag="ACGEXS_000972"
     CDS             complement(2706..3215)
                     /gene="msrA"
                     /locus_tag="ACGEXS_000972"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFF8011993.1"
                     /translation="MNINTAYFAGGCFWCMTKPFDTFDGIEKVTSGYMGGHIENPTYE
                     QVKSGTSGHLETVEIQYDVALFSYNKLLEIFFSVIDPLDTGGQYQDCGPQYQTAIFYT
                     NDHQKELAETYIEQLKNTINADKAIATKILPASQFYKAEDYHQDFYKKNPERYAEEQK
                     IRQEYKNKQ"
     gene            3352..4335
                     /locus_tag="ACGEXS_000973"
     CDS             3352..4335
                     /locus_tag="ACGEXS_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFF8011994.1"
                     /translation="MDKETNDNEYRRQSEHRTSAPKRKKKKKIRKLPIILLIVVILLI
                     ALVVYIVHSYNSGVEYAKKHAKDVKVHQFNGPVKNDGKISILVLGADKAQGGQSRTDS
                     IMVVQYDFINKKMKMMSVMRDIYADIPGYGKHKINSAYALGGPELLRKTLDKNLGINP
                     EYYAVVDFTGFEKMIDELMPEGVPINVEKDMSKNIGVSLKKGNHRLNGKELLGYARFR
                     HDPEGDFGRVRRQQQVMQTLKKEMVNFRTVVKLPKVAGILRGYVNTNIPDSGIFQTGL
                     SFGIRGEKDVKSLTVPIKNSYEDVNTNTDGSALQINKNTNKQAIKDFLDED"
     gene            complement(4423..4608)
                     /locus_tag="ACGEXS_000974"
     CDS             complement(4423..4608)
                     /locus_tag="ACGEXS_000974"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFF8011995.1"
                     /translation="MPIVNVKLLEGRSDEQLKNLVSEVTDAVEKTTGANRQAIHVVIE
                     EMKPNHYGVAGVRKSDQ"
     gene            4756..6018
                     /locus_tag="ACGEXS_000975"
     CDS             4756..6018
                     /locus_tag="ACGEXS_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFF8011996.1"
                     /translation="MYNYHLLEDRDVLCIDQKSFFASVSCIEKGLDPLETKLAVVADT
                     KRQGSVILAATPKLKELGIKTGSRLFEIPHRNDIYIINPSMRKYLNVSVAISKIALRY
                     IPPEDLHQYSIDEFFMDVTDSYHRFSSTVHAFCERLKREIYEETGIYCTVGIGSNMLL
                     SKIAMDVEAKHSQNGIAEWRYQDVPTKLWPIQPLRDFWGINRRTEAKLNKRGIFTIGD
                     LAKYPYKFLKKEFGILGVDMHLHANGIDQSKVREKHKISNPSICKSQILMRDYHFDEA
                     KVVMQELIEDVASRVRARKKVARTIHFAFGYSDEGGVHKQYTLKDPTNLEKDIYKVVM
                     HFADKLCNKQALYRTLSISLSQFINEDERQLSLFEDEYQRKRDECLAKTIDQLHLKCG
                     KGIVSKAVSFTEAGTKHGRLGLMAGHKM"
     gene            complement(6155..7246)
                     /locus_tag="ACGEXS_000976"
     CDS             complement(6155..7246)
                     /locus_tag="ACGEXS_000976"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFF8011997.1"
                     /translation="MTTVLFVGLGLIGGSLASNIKYHNPNTNIIAYDADTSQLDKAKS
                     IGIINEKCLNYSEAIKKADVIIYATPVAITNKYLSELIDMPTKPGVIVSDTGSTKAMI
                     QQHECNLLKHNIHLVSGHPMAGSHKSGVLNAKKHLFENAYYILVYNEPRNEQAANTLK
                     ELLSPTLAKFIVTTAEEHDYVTSVVSHLPHIVASSLVHVSQKNGQEHHLVNKLAAGGF
                     RDITRISSSNAQMWKDITLSNKTYILEMIRQLKSQFQDLERLIESNDSEKLLSFFAQA
                     KSYRDALPAKQLGGLNTAYDLYVDIPDESGMISKVTYILSLHNISISNLRILEVREDI
                     YGALKISFKNPTDRERGMQALSDFDCYIQ"
     gene            7411..8442
                     /locus_tag="ACGEXS_000977"
     CDS             7411..8442
                     /locus_tag="ACGEXS_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFF8011998.1"
                     /translation="MEPILEMIKTLTGINSPSGDTEEAIQFVEKYAKDLGYQTTLTNK
                     GALLITVPGKNDEVQRCITAHVDTLGAMVKEIKEDGRLAIELIGGFTYNAIEGEYCQI
                     KTDAGQIYTGTICLHETSVHVYRNNHEIPRDQKHMEIRIDEVTTSEEDTKSLGISVGD
                     FVSFDRRTVITSSGFIKSRHLDDKASVAMILQLLKKLKEEQIILPHTTQFYISNNEEI
                     GYGANASIDSKIKEYIALDMGALGDGQASDEYTVSICAKDASGPYHKQLKLHLVNLCK
                     INNIPYKVDIYPYYGSDASAALHAGADIRHGLFGAGIESSHAMERTHIDSIKATEKLL
                     YAYCLSPIE"
     gene            8934..10340
                     /locus_tag="ACGEXS_000978"
     CDS             8934..10340
                     /locus_tag="ACGEXS_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFF8011999.1"
                     /translation="MDIFYKKIKANVTPEVLAQLHSKKIILESTNQQQTKGRYSVVIF
                     DIYGTLTLDNDVLSVSTLKESYQITERPYHYLTTKINEDYHNIQDEQLKSLPFISGYV
                     GTCSFDLVRHEFPKLQSIQLEDHKQHDVRLYMVEQVYVFDHYKDELYIIATNQFSNST
                     KSDLENRVIKSIEDLTKIQPFMPTQGFDFKTKEIQSNISEERFIEMIQYFKEKITEGD
                     MFQVVPSRIYKYAHHASQHLNQLSFQLYQNLKRQNPSPYMYYLNIDQPYIVGSSPESF
                     VSVKDQIVTTNPIAGTIQRGETTQIDNENMKQLLNDPKECSEHRMLVDLGRNDIHRVS
                     KIGTSKITKLMVIEKYEHVMHIVSEVTGKINQNLSPMTVIANLLPTGTVSGAPKLRAI
                     ERIYEQYPHKRGVYSGGVGYINCNHNLDFALAIRTMMIDEQYINVEAGCGVVYDSIPE
                     KELNETKLKAKSLLEVSP"
     gene            10337..10903
                     /locus_tag="ACGEXS_000979"
     CDS             10337..10903
                     /locus_tag="ACGEXS_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HFF8012000.1"
                     /translation="MILVVDNYDSFTYNLVDIVAQHTDVIVQYPDDDNVLNQSVDAVI
                     ISPGPGHPLDDQQLMKIISTYQHKPILGICLGAQALTCYYGGEVIKGDKVMHGKVDTL
                     KVISHHQHLLYQDIPEQFSIMRYHSLISNPDNFPEELKITGRTKDCIQSFEHKERPHY
                     GIQYHPESFATDYGVKIITNFINLVKEG"
     gene            10909..11907
                     /gene="trpD"
                     /locus_tag="ACGEXS_000980"
     CDS             10909..11907
                     /gene="trpD"
                     /locus_tag="ACGEXS_000980"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HFF8012001.1"
                     /translation="MTLLTRIKTETILLESDIKELIDILISPSIGTDIKYELLSSYSE
                     REIQQQELTYIVRSLINTMYPHQPCYEGAMCVCGTGGDKSNSFNISTTVAFVVASAGV
                     KVIKHGNKSITSNSGSTDLLNQMNIQTTTVDDTPNQLNEKDLVFIGATESYPIMKYMQ
                     PVRKMIGKPTILNLVGPLINPYHLTYQMVGVFDPTKLKLVAKTIKDLGRKRAIVLHGA
                     NGMDEATLSGDNLIYELTEDGEIKNYTLNATDYGLKHAPNSDFKGGSPEENLAISLNI
                     LNGKDQSSRRDVVLLNAGLSLYVAEKVDTIAEGIELATTLIDNGEALEKYHQMRGE"
     gene            11909..12691
                     /gene="trpC"
                     /locus_tag="ACGEXS_000981"
     CDS             11909..12691
                     /gene="trpC"
                     /locus_tag="ACGEXS_000981"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HFF8012002.1"
                     /translation="MTILSEIVKYKQSLLQNVYYQDKLNTLKSVKIQNKKSFINAIEK
                     EPKLAIIAEIKSKSPTVNDLPERDLSQQISDYDQYGANAVSILTDEKYFGGSFERLQA
                     LTTKTTLPVLCKDFIIDPLQIDVAKQAGASMILLIVNILSDKQLKDLYNYAISQNLEV
                     LVEVHDRHELERAYKVNAKLIGVNNRDLKRFVTNVEHTNTILENKKTNHYYISESGIH
                     DASDVRKILHSGIDGLLIGEALMRCDNLSEFLPQLKMQKVKS"
     gene            12691..13323
                     /locus_tag="ACGEXS_000982"
     CDS             12691..13323
                     /locus_tag="ACGEXS_000982"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HFF8012003.1"
                     /translation="MKLKFCGFTSIKDVTAASQLPIDAIGFIHYEKSKRHQTITQIKK
                     LASAVPNHIDKVCVMVNPDLTTIEHVLSNTSINTIQLHGTESIDFIQEIKKKYSSIKI
                     TKALAADENIIQNINKYKGFVDLFIIDTPSVSYGGTGQTYDWTILKHIKDIPYLIAGG
                     INSENIQTVNQLKLSHQGYDLASGIEVNGRKDIEKMTAIVNIVKGDRDNE"
     gene            13316..14530
                     /gene="trpB"
                     /locus_tag="ACGEXS_000983"
     CDS             13316..14530
                     /gene="trpB"
                     /locus_tag="ACGEXS_000983"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFF8012004.1"
                     /translation="MNKQIQTEADELGFFGEYGGQYVPETLMPAIIELKKAYKEAKAD
                     PEFQRELEYYLSEYVGRATPLTYAASYTESLGGAKIYLKREDLNHTGAHKINNALGQA
                     LLAKRMGKKKLVAETGAGQHGVASATVAALFDMELVVFMGSEDIKRQQLNVFRLELLG
                     AKVVAVEDGQGTLSDAVNKALQYWVSHVDDTHYLLGSALGPDPFPTIVRDFQSVIGKE
                     IKSQILKKEGRLPDAIVACIGGGSNAIGTFYPFIKDDVALYGVEAAGQGDDTDKHALA
                     IGKGSPGVLHGTKMYLIQDEDGQVQLAHSISAGLDYPGIGPEHSYYHDIGRVTFENAS
                     DTQAMNALINFTKHEGIIPAIESAHALSYVERLAPTMSKEDIIVVTISGRGDKDMETI
                     RQYMVERGLAND"
     gene            14523..15251
                     /gene="trpA"
                     /locus_tag="ACGEXS_000984"
     CDS             14523..15251
                     /gene="trpA"
                     /locus_tag="ACGEXS_000984"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFF8012005.1"
                     /translation="MTKLFIPYIMGNKDLIENATLLSENGADIIEIGVPFSDPVADGP
                     VIMEAGQQAIKQGITIDYIFNQLEKHGDQIKCNYVLMTYYNIICHYGEQAFFEKCRDT
                     GVYGLIIPDLPYELSQRLKQQFSHYGVKIISLVVMTTDDKRIKDIVSHAEGFIYTVTM
                     NATTGQNGAFHPELKRKIESIKAIANVPVVAGFGIRTPQHVADIKEVADGIVIGSEIV
                     KRFKSNTREEIIKYLQSIQQTLNN"
     gene            15573..16835
                     /gene="femA"
                     /locus_tag="ACGEXS_000985"
     CDS             15573..16835
                     /gene="femA"
                     /locus_tag="ACGEXS_000985"
                     /EC_number="2.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine glycyltransferase FemA"
                     /protein_id="HFF8012006.1"
                     /translation="MKFTNLTAKEFGAFTDSMPYSHFTQTVGHYELKLAEGYETHLVG
                     IKNNNNEVIAACLLTAVPVMKVFKYFYSNRGPVIDYENQELVHFFFNELSKYVKKHRC
                     LYLHIDPYLPYQYLNHDGEITGNAGNDWFFDKMSNLGFEHTGFHKGFDPVLQIRYHSV
                     LDLKDKTADDIIKNMDGLRKRNTKKVKKNGVKVRFLSEEELPIFRSFMEDTSESKAFA
                     DRDDKFYYNRLKYYKDRVLVPLAYINFDEYIKELNEERDILNKDLNKALKDIEKRPEN
                     KKAHNKRDNLQQQLDANEQKIEEGKRLQEEHGNELPISAGFFFINPFEVVYYAGGTSN
                     AFRHFAGSYAVQWEMINYALNHGIDRYNFYGVSGKFTEDAEDAGVVKFKKGYNAEIIE
                     YVGDFIKPINKPVYAAYTVLKKVKDRIF"
     gene            16854..18113
                     /gene="femB"
                     /locus_tag="ACGEXS_000986"
     CDS             16854..18113
                     /gene="femB"
                     /locus_tag="ACGEXS_000986"
                     /EC_number="2.3.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine glycyltransferase FemB"
                     /protein_id="HFF8012007.1"
                     /translation="MKFTELTVTEFDNFVQNPSLESHYFQVKENIVTRENDGFEVVLL
                     GIKDDNNKVIAASLFSKIPTMGSYVYYSNRGPVMDFSDLGLVDYYLKELDKYLQQHQC
                     LYVKLDPYWLYHLYDKDIVPFEGREKNDALVNLFKSHGYEHHGFTTEYDTSSQVRWMG
                     VLNLEGKTPETLKKTFDSQRKRNINKAINYGVKVRFLERDEFNLFLDLYRETEERAGF
                     VSKTDDYFYNFIDTYGDKVLVPLAYIDLDEYVLKLQQELNDKENRRDQMMAKENKSDK
                     QMKKIAELDKQIDHDQHELLNASELSKTDGPILNLASGVYFANAYEVNYFSGGSSEKY
                     NQFMGPYMMHWFMINYCFDNGYDRYNFYGLSGDFTENSEDYGVYRFKRGFNVQIEELI
                     GDFYKPIHKVKYWLFTTLDKLRKKLKK"
     gene            18796..19563
                     /locus_tag="ACGEXS_000987"
     CDS             18796..19563
                     /locus_tag="ACGEXS_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFF8012008.1"
                     /translation="MRFVFDIDGTLCFDGRLIDQTIIDTLLQLQHDGHELIFASARPI
                     RDLLPVLPSVFHQHTLIGANGAMISQQSKISVIKPIHTDTYHHIFKIIQKYELDYIID
                     DDWNYAAQLDAENAIFERLDPHKLASCIDVANIDTPIKIILLNIDPAQITTILDELDK
                     YHQELEMIHHSNEYNIDITAQNINKYTALQYIFDADVKYIAFGNDHNDIVMLQHASSG
                     YIIGPSEAYTHAILKLDKIKHINNNAQAICKVLKSYK"
     gene            complement(19611..>20082)
                     /locus_tag="ACGEXS_000988"
     CDS             complement(19611..>20082)
                     /locus_tag="ACGEXS_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SWIM zinc finger domain-containing protein"
                     /protein_id="HFF8012009.1"
                     /translation="GLLDIASIRSIERGFNYYQSECVINLKSFSETQHEAEVKGSGNK
                     VYRCYIDMEHPRKSICNCPHADGRRVICKHMIALLFTASPEAANKHIMMLNEVEEDYQ
                     LRRNMWIDSLKEMINDMSEEELRDAYLNMLIEHGEMAELFGLDEEEEMFEDEFY"
BASE COUNT         7086 a         2914 c         3596 g         6486 t
ORIGIN      
        1 caaaatattt tcaatcttaa aaattacgtt tgctacagct ttatttattt ttgtagcaat
       61 cacattgtat cgggagttat ctggtattaa ctttaaagat acgttggttg aatttagtaa
      121 gattaaccgt atgtccttag tgttactatt tattggtggt ggggcatcgc ttgttattct
      181 atcaatgtat gatgtgattt tatctagagc tttaaaaatg gatatatcct taggcaaagt
      241 tttaagagta agttatatca tcaatgcatt gaatgcgatt gtaggtttcg gtggctttat
      301 tggtgcaggc gttagagcaa tggtttataa aaactatacg catgataaaa agaaattagt
      361 tcactttata tccttaatac ttatttcaat gttgacaggt ttaagcttat tatcattgct
      421 aattgtattc catgttttcg atgcatcttt aatcttagat aagattacat gggtaagatg
      481 ggtattatat gtagtgtcat ttttcttacc attattcatt atttattcaa tggttagacc
      541 acccgataaa aacaatcgtt ttgtaggatt gtactgcact ttagtgtcgt gtgttgaatg
      601 gttagcagct gcagttgtat tatatttctg tggtgtaatt gttgacgctc atgtatcatt
      661 catgtccttt attgcaatat ttatcattgc tgcattatca ggtttagtca gctttattcc
      721 tggtggtttc ggcgctttcg atttagttgt attactagga tttaaaactt taggtgtccc
      781 tgaggaaaaa gtattattaa tgctacttct atatcgtttt gcgtactatt ttgtaccggt
      841 aattattgca ttaattttat catcatttga atttggtaca tcagctaaga agtacattga
      901 gggatctaaa tactttattc ctgctaaaga tgttacgtca tttttaatgt cttatcaaaa
      961 ggatattatt gctaaaattc catcattatc attagcaatt ttagtattct ttacaagtat
     1021 gatctttttt gtaaataact taacgattgt ttacgatgct ttatatgatg gaaatcactt
     1081 aacgtattat attctattgg caattcatac tagtgcttgt ttattacttt tactgaatgt
     1141 agttggtatt tataagcaaa gtagacgtgc cattatcttt gctatgattt caattttatt
     1201 aatcacagtg gcgacattct tcacttacgc ttcatatatt ttaataacat ggttagctat
     1261 tatttttgtt ctgcttattg tagctttccg tagagcacgt aggttgaaac gcccagtaag
     1321 aatgagaaat atagttgcaa tgcttttatt cagtttattt attttatatg ttaaccatat
     1381 atttattgct ggaacgttat atgcattaga tatttatacg attgaaatgc atacatctgt
     1441 attgcgctat tacttctggc ttacgatttt aatcatcgct atcatcatag gtatgattgc
     1501 atggttgttt gattatcaat ttagcaaagt acgtatttct tctaaaattg aagattgcga
     1561 ggagattatt aatcagtacg gcggtaatta tttgagtcac ttgatatata gtggtgacaa
     1621 gcagtttttc actaatgaaa ataaaacagc atttttaatg tatcgttata aagcaagttc
     1681 attagtggtt cttggagatc cgttaggtga tgaaaatgcc tttgatgaat tgttagaagc
     1741 attctataat tacgctgagt atttaggcta tgatgttata ttctatcaag ttacagatca
     1801 acacatgcct ttatatcata atttcggtaa ccaatttttc aaattaggtg aagaagcaat
     1861 tattgattta acgcaatttt caacttcagg taaaaaacgc cgtggattta gagcgacttt
     1921 aaataaattc gatgaactta atatttcgtt cgaaattatt gaaccaccgt tttcaactga
     1981 atttataaat gaacttcaac atgtaagtga tttatggcta gataatcgtc aggaaatgca
     2041 tttctctgtt ggtgaattta atgaagaata cttatctaaa gcgccaattg gtgtaatgcg
     2101 aaatgaagaa aatgaagtaa ttgcattttg tagtttaatg ccaacatact ttaatgatgc
     2161 catttcagtc gatttaatta gatggttgcc agagttagat ttaccattaa tggatggtct
     2221 atacttgcat atgttacttt ggagtaaaga acaaggttat acaaaattta atatgggtat
     2281 ggcaacgtta tcgaacgttg gtcaattgca ttattcatat ttaagagaac gacttgcagg
     2341 ccgtgtcttt gaacatttca acggtctata tcgtttccaa ggattacgtc gttataaatc
     2401 taaatataat ccgaattggg aaccacgctt tttagtttat cgtaaagata attcgctttg
     2461 ggaatcactt tctaaagtaa tgcgtgtaat acgtcacaaa taattaaaat ccaagtgcta
     2521 agaggtatac agttatgcct gatgcgcttg gattttttgg tttctataaa gaatgattta
     2581 taaaaaacgg tacagataag aatatttaag tttcctatct gtaccgtatt ttaatattga
     2641 aatgagctaa agttaagtcg cctaactttt attatcagtc caatcagttt catcaagtat
     2701 aaatgttatt gcttattttt gtattcttgg cgtatttttt gttcttctgc atagcgctct
     2761 ggatttttct tataaaaatc ttggtgatag tcttcggctt tgtaaaattg tgacgctggt
     2821 aatatttttg ttgcaattgc cttatcagca ttaatcgtat ttttaagctg ctcgatataa
     2881 gtctcagcga gttctttttg atgatcatta gtgtagaaaa tagctgtttg atattgagga
     2941 ccacagtctt gatattgacc acctgtatct aatgggtcaa tgactgagaa aaatatttct
     3001 aataacttat tgtatgagaa taatgcaaca tcatattgaa tttcaacagt ttctaaatga
     3061 ccactcgtac ctgattttac ttgttcgtaa gtaggatttt caatatgtcc gcccatatat
     3121 ccagaagtta ctttttctat gccgtcaaag gtgtcaaatg gtttcgtcat acaccaaaag
     3181 caacctccgg caaaataagc tgtattaata ttcatttttg acatcctttc attagacctt
     3241 agtacgattt attaagaaat cacttgcttt ttgaattgtt tttatataac gttaatatgt
     3301 gattattata gtacacaaaa atctattttt gaagatataa ggtaggtaaa tatggataaa
     3361 gaaactaatg acaacgaata tagacgtcaa agtgaacatc gcacttcggc gcctaagcga
     3421 aaaaagaaga agaaaattag gaaattacct atcattcttc tgattgttgt aattttactt
     3481 atcgcattag ttgtatatat tgtacatagt tacaatagcg gtgtagaata tgccaagaaa
     3541 catgcgaaag atgttaaagt acatcaattt aatggaccag taaaaaatga tggtaaaatt
     3601 tctattcttg tactcggtgc agataaagca caaggtggac aatcaagaac agattctatc
     3661 atggttgttc aatatgactt tatcaataaa aagatgaaaa tgatgtctgt catgcgtgat
     3721 atttatgcag atattccagg atatggaaaa cacaaaatta attcagcata cgctttaggt
     3781 ggtccagagc tacttagaaa aacacttgat aaaaatttag gaattaatcc tgaatattat
     3841 gcagtagttg attttactgg atttgagaaa atgattgatg aattaatgcc tgaaggtgta
     3901 ccaattaatg tcgaaaaaga tatgtcgaaa aatattggtg tatctttgaa aaagggtaac
     3961 cataggttga atggtaaaga attacttggt tatgcaagat tccgtcacga ccctgaaggt
     4021 gacttcggac gcgtgcgacg tcagcaacaa gtgatgcaaa cattgaaaaa agaaatggtt
     4081 aattttagaa cagttgttaa attaccaaaa gttgcaggta ttttaagagg ctatgtgaat
     4141 acaaacattc ctgattcagg gattttccaa acaggtttga gttttggtat ccgaggtgaa
     4201 aaagatgtta agtcattgac tgtgccaatc aagaactcat acgaagatgt caatacaaat
     4261 actgatggta gtgcattaca gattaataaa aacacaaata aacaagctat taaagacttt
     4321 ttagatgaag attaaaaata aacaaggcga tttctatcat actcagatag aagtcgcctt
     4381 gttcgttttc aaagaattaa ttggcatgca tcttatgaag aattattgat ctgactttct
     4441 tacgccagcc acaccataat ggtttggttt catttcttct ataacaacgt gaattgcttg
     4501 tctatttgcc cccgttgttt tttctacggc gtcagttact tcgctaacta aattttttaa
     4561 ttgttcatcc gaacgacctt ctaataattt tacattgacg attggcatca tccaaacctc
     4621 ctaatcatta ttaatttgag tatatcatgt aatacttaaa aattcatttg caaaataaca
     4681 gaacatttgt tctaaaatag ttgaaataga acacgtgttc gtatataata agattcaagt
     4741 aagggaggaa taacagtgta taattatcat ttattagaag atagggatgt tctatgtatt
     4801 gaccaaaaaa gtttttttgc gagtgtttct tgtattgaaa aggggctaga tccattagaa
     4861 acaaagctag ctgttgttgc agatactaag cgtcagggtt ctgtaatatt ggctgcgaca
     4921 cctaaattaa aagaattagg catcaagaca gggtcgcgat tgtttgaaat accacataga
     4981 aatgatattt acattatcaa tccaagtatg cgtaaatatc ttaatgtttc agttgctatt
     5041 tctaagattg cattgcgtta tattccacct gaagatttac accaatatag tattgacgaa
     5101 ttttttatgg atgttactga tagctatcat agatttagtt ctacagtaca tgcattttgc
     5161 gaaagactta aacgtgaaat ttatgaagaa acaggcattt attgtactgt gggcattggt
     5221 tctaatatgt tattaagtaa aattgctatg gatgttgaag cgaagcatag tcaaaatggt
     5281 atagctgaat ggcgatatca agatgtacca acgaaattat ggccaattca gcccttgcga
     5341 gatttttggg gtattaatcg tcgaacagaa gccaaattga ataaaagagg aatttttact
     5401 ataggagatt tagcgaaata tccatataaa tttttaaaaa aagagttcgg tattttaggt
     5461 gttgatatgc atctacatgc gaatgggata gatcagagta aagtacgtga aaagcacaag
     5521 atcagcaatc catcgatatg caaaagtcaa atattaatga gagattatca ttttgatgaa
     5581 gcaaaagtag taatgcaaga gttaattgaa gatgttgcta gcagagttcg agcaagaaaa
     5641 aaagtggcaa gaacgataca ttttgccttt ggctatagtg atgaaggcgg tgtacataag
     5701 caatatactt tgaaagatcc aacaaactta gaaaaagata tttataaagt agtaatgcat
     5761 ttcgcagata aattatgtaa taaacaagca ctatatcgta cgctaagtat atctttgagt
     5821 caatttatta atgaggatga gcgacagtta agtctgtttg aagatgaata ccaacgcaaa
     5881 cgtgacgaat gtctagctaa aacgatagac caattacatt tgaaatgcgg caaaggtatt
     5941 gtgtccaaag cagtatcgtt tacagaagca ggtacaaaac acggcagatt aggtttaatg
     6001 gctggacata aaatgtaatg actatacggt ttaagtaata tataactgtg attcgtataa
     6061 aataagtctc taaagataaa tatttcatat atcacaatag attttcacaa taatatctaa
     6121 gaatacatgg aatttatcaa aagagactta ataattattg gatataacaa tcaaaatcac
     6181 tcaatgcttg cataccgcgt tctcggtcag tagggttttt gaaactaatt tttaaagcac
     6241 cgtatatatc ttcgcgtact tctaagattc ttaagttgct tatagatatg ttatgtaaac
     6301 tcaggatata agtcacttta cttatcatac ctgattcatc cggaatgtct acatatagat
     6361 catacgcagt atttagtcca cctagttgtt tagcgggtag tgcgtcgcga tacgatttag
     6421 cttgggcaaa aaatgataac aatttttcag aatcattgct ttcaattagt ctttctaaat
     6481 cttgaaactg actttttagc tgtcgaatca tttctaaaat atacgtttta ttactcaagg
     6541 tgatatcttt ccacatttgt gcattactac tagatatacg agtgatatca cgaaaaccac
     6601 cagctgcaag tttattaact aaatgatgtt cttgaccgtt cttttgacta acatgaacta
     6661 aactagatgc aacgatatga ggtaaatgac ttacgacgct tgttacgtag tcgtgttctt
     6721 cagcagtagt tacaataaat ttagcaagag taggtgataa cagttctttt aacgtgtttg
     6781 ctgcttgctc atttcttggc tcattgtaga ctaaaatata ataagcgttt tcaaataagt
     6841 gctttttagc atttagtaca ccagatttat gactaccagc cattggatga ccactgacta
     6901 aatgaatatt atgctttaat aaattgcatt cgtgttgctg tatcattgct ttagtactac
     6961 cagtatcaga aacaataaca ccaggtttag ttggcatatc tataagctcg ctaagatatt
     7021 tatttgtgat agcaacaggt gttgcataaa taattacatc ggctttttta atagcttcac
     7081 tataatttaa acatttttca ttaataatgc cgattgattt agctttatct aactgagaag
     7141 tatctgcatc gtatgcaata atattagtat tagggttatg gtattttata ttgctagcaa
     7201 gacttccacc aattaatcca agcccaacaa ataaaactgt tgtcatataa atcaccttat
     7261 ttcgaaattt tcagaataat aacattgtaa atgagctgtt gacacagtgc aatagtaaat
     7321 aaaaatcgat aatagcatta atagataaac ggagataaat catctacaat aaagagtata
     7381 gtaacacaat ggcaacggag gggtaaatca atggaaccaa tattagaaat gattaaaaca
     7441 ttaacaggta ttaatagtcc ttcaggagac acagaagaag caattcaatt tgtcgaaaaa
     7501 tatgcaaaag acttgggtta tcaaacaaca ctaacaaata aaggtgcgtt attaataaca
     7561 gtgccaggca aaaatgatga agtacaacgc tgtattactg ctcatgttga tactttaggt
     7621 gcaatggtta aagaaattaa agaagatggt cgcttagcaa tagaattaat tggaggattc
     7681 acgtataacg cgattgaggg tgaatattgc caaattaaaa ctgatgctgg tcaaatatat
     7741 acaggaacaa tttgtctgca tgaaacaagt gttcatgtat atagaaataa tcatgaaata
     7801 cctagagatc aaaagcatat ggaaataaga attgatgaag taactacatc agaagaagat
     7861 acaaagagtt taggtatttc agtaggtgat tttgttagct ttgatcgacg tacagttatc
     7921 acgtcatcag gttttattaa atctcgtcat ttagatgata aagctagcgt agcgatgata
     7981 ctacaattac taaagaaatt aaaagaagag caaataatat taccacatac aacgcaattt
     8041 tatatttcta ataacgaaga aataggttac ggtgcaaatg catcaattga ttcgaaaatc
     8101 aaagaatata ttgcattaga tatgggcgcg ttgggagacg gtcaagcatc ggatgaatat
     8161 acagtttcta tttgtgccaa agatgcttca ggtccatatc ataagcaatt gaaattgcac
     8221 ctagttaatc tttgcaaaat aaataacatt ccatataaag tagacatata tccatattat
     8281 ggttcagatg cttcagcagc tttacatgct ggtgcggata tcagacatgg tttatttggc
     8341 gctggcattg aatcatctca tgcaatggaa cgaacacata ttgattctat taaagcgaca
     8401 gagaaattac tatatgcata ttgcttatca ccaattgagt aaacaattag tgttgacaaa
     8461 tgtgaacgac ctatgtaata taatgaacta taaaaataat tagaattttc taaagaaata
     8521 gtagcagata tgaaacgtag caaatagaaa gctaatgggt gatgggaatt agcacgccat
     8581 atcttgtgaa ttggactttg gaaaacaatt gaatgagttt tgaaagtgaa catgaattat
     8641 gttaactaag gtggcaccac ggtaacgcgt ccttacaggt atatgcgtta tgtggtgtct
     8701 ttttatttag acaaaatgta gtagttaatt aaaggtagca acagaaagtt agtggatgat
     8761 gtgaactaac accgagatta atgaaattgg gttttgtctg caacagaaaa attatatata
     8821 gtaaagagtg aactatgaat atttcgaata ttcggttaat ttaggtggta ccacgcgtca
     8881 gcgtccttta tattgataag gatgctggcg cttttttgaa aggagcgtat agaatggata
     8941 tattttataa aaaaataaaa gcaaatgtaa cgcccgaagt tttagcacaa cttcattcca
     9001 agaagatcat tttggaaagt acaaatcaac aacaaactaa aggtcgctat tcagttgtta
     9061 tttttgatat ttatggcact ttaactttag ataatgatgt attatcagta agtactttaa
     9121 aagaatcgta tcaaatcact gaaagaccgt accattattt aacgactaaa ataaatgaag
     9181 actaccataa tattcaagat gagcaactta agtcattacc atttatatct ggatatgttg
     9241 ggacgtgtag ctttgattta gtaagacatg aatttcctaa attgcaatca atacaattag
     9301 aagatcacaa gcagcacgat gtaaggttat atatggttga acaagtttat gtatttgacc
     9361 attacaaaga tgagttatat atcatcgcga cgaatcaatt ttcaaattca acaaaatcag
     9421 atcttgagaa tcgagttatt aagtctatcg aagacttaac taaaatccaa ccattcatgc
     9481 ctacacaagg ttttgatttt aaaactaaag aaattcaatc aaacatttct gaagaaagat
     9541 ttatcgaaat gattcagtat ttcaaagaga aaataacaga aggggatatg ttccaagttg
     9601 tgccatcaag aatttacaaa tatgcgcatc atgctagtca gcatttaaat caactttcgt
     9661 ttcaactgta tcaaaattta aaacgacaaa acccaagtcc atatatgtat tatcttaata
     9721 tcgatcaacc atatattgtc ggtagttctc ccgaaagttt cgtaagtgtc aaagatcaaa
     9781 ttgtaacaac taatcctatt gcaggtacga ttcaacgtgg tgagacgaca caaatagata
     9841 atgagaatat gaaacaacta cttaatgatc caaaagaatg cagcgaacat cgtatgctag
     9901 ttgatttagg acgtaatgat attcatagag taagtaaaat cggcacctca aaaattacta
     9961 aattaatggt tattgaaaaa tatgaacatg ttatgcatat cgtaagtgaa gtcacaggta
    10021 aaataaatca aaatttatcg ccaatgacag ttattgcgaa tttattacca acaggtaccg
    10081 tttcaggtgc accaaaatta cgtgcaattg aaagaatata tgaacaatat ccacataaac
    10141 ggggcgttta tagtggtggt gttggataca taaattgtaa tcataactta gattttgcat
    10201 tagcaattcg aacgatgatg atagatgagc agtatatcaa cgtagaagct ggttgtggcg
    10261 ttgtatatga ttctattcct gaaaaagaac tgaatgaaac gaaattgaaa gctaaaagct
    10321 tattggaggt gagcccatga tcttagttgt agataattat gattccttta catataacct
    10381 agtggatatt gttgctcaac atactgacgt cattgttcaa taccctgatg atgataatgt
    10441 gctgaatcaa tcggtggacg ctgttattat atctcctggt ccagggcatc cattagacga
    10501 tcaacagtta atgaaaatca tatcaaccta tcaacacaaa cccattttag gtatttgttt
    10561 aggggctcag gcactgactt gttactacgg tggagaagtc attaaaggcg acaaggttat
    10621 gcacggcaaa gttgatacac taaaggttat atcgcatcat caacatctgt tatatcaaga
    10681 tataccagaa cagttttcaa ttatgagata tcattcatta ataagtaacc ctgacaattt
    10741 tccagaagaa ttgaaaatta ctggacgtac caaagattgt atacagtcat tcgagcataa
    10801 agaaagaccg cattatggta ttcagtacca tcctgaatca tttgctacag actatggtgt
    10861 caaaataatt acaaatttca ttaatctagt gaaggaagga tgaaaaccat gacattacta
    10921 acaagaataa aaactgaaac tatattactt gaaagcgaca ttaaagagct aatcgatata
    10981 cttatttctc ctagtattgg aactgatatt aaatatgaat tacttagttc ctattcggag
    11041 cgagaaatcc aacaacaaga attaacatat attgtacgta gcttaattaa tacaatgtat
    11101 ccacatcaac catgttatga aggggctatg tgtgtgtgcg gcacaggtgg tgacaagtca
    11161 aatagtttca acatttcaac gactgttgct tttgttgtag caagtgctgg cgtaaaagtt
    11221 ataaaacatg gtaataaaag tattacctca aattcaggta gtacggattt gttaaatcaa
    11281 atgaacatac aaacaacaac tgttgatgat acacctaacc aattaaatga aaaagacctt
    11341 gtattcattg gtgcaactga atcatatcca atcatgaagt atatgcaacc agttagaaaa
    11401 atgattggaa agcctacaat attaaacctt gtgggtccat taattaatcc atatcactta
    11461 acgtatcaaa tggtaggcgt ctttgatcct acaaagttaa agttagttgc taaaacgatt
    11521 aaagatttag gtagaaaacg tgcaatcgtt ttacatggtg caaatggtat ggatgaagca
    11581 acactatctg gtgataattt gatatatgaa ttgactgaag atggagaaat caaaaattac
    11641 acattaaatg cgactgatta tggtttgaaa catgcgccga atagtgattt taaaggcggt
    11701 tcacctgaag aaaatttagc aatctccctt aatatcttga atggtaaaga tcagtcaagt
    11761 cgacgtgatg ttgtcttact aaatgcgggt ttaagccttt atgttgcaga gaaagtggat
    11821 accatcgcag aaggcataga acttgcaact acattgattg ataatggtga agcattggaa
    11881 aaataccatc aaatgagagg tgaataatat gacgatttta tcagaaattg ttaaatataa
    11941 acagtcactt ttacaaaatg tctattatca agacaaactt aataccttga aaagtgtgaa
    12001 gattcagaat aaaaaatctt ttataaacgc aattgagaaa gaaccaaagc tagcaattat
    12061 tgcagaaatt aaatcgaaga gtcctacagt taatgactta cctgaacgag atttatcgca
    12121 acaaatctca gattatgacc aatatggtgc aaatgccgtg tccattttaa ctgatgaaaa
    12181 gtactttggt ggtagttttg aaagattaca agcattgacg acaaaaacaa cattacccgt
    12241 attatgcaaa gactttatta tagacccgct tcaaattgat gttgctaaac aagctggtgc
    12301 atctatgatt ttattgatcg ttaacatctt atctgataaa caattgaaag atttatataa
    12361 ctacgctata tcgcaaaatc tagaagtgtt agttgaagta catgatcgcc atgaattaga
    12421 acgtgcctat aaggttaatg ctaaattgat tggtgtaaat aacagggact taaaacgatt
    12481 tgttacaaat gtggaacata caaatactat tttagaaaat aaaaaaacaa atcattatta
    12541 tatttctgaa agtggtattc acgatgcatc tgatgtaaga aaaatcttgc atagtggtat
    12601 cgatggctta ctaataggtg aggcgcttat gcgttgtgac aatctatctg aatttttacc
    12661 acaactgaaa atgcaaaagg tgaagtcatg atgaaattga aattttgtgg ctttacatca
    12721 ataaaggatg ttacagcggc cagtcaatta cctattgatg cgataggttt catccattat
    12781 gaaaaaagta aaaggcatca aacaattacc caaataaaaa agttagcgtc tgctgttcca
    12841 aatcatatcg ataaagtatg tgtcatggta aatcctgatt taacaacaat tgaacacgta
    12901 ttaagcaata cgtcaattaa cacaatacag ttacacggca cagaatctat tgattttata
    12961 caggaaatta aaaagaaata ttcaagcatt aaaatcacta aagctttagc tgcagatgaa
    13021 aacataatcc aaaacataaa taaatataaa gggttcgtag atttatttat tatcgacaca
    13081 ccctcagtgt cgtatggtgg taccggtcaa acatatgact ggactatttt gaagcacata
    13141 aaagacatac cttatttgat agcaggaggc attaactctg aaaatattca aacagttaat
    13201 caacttaaat tatcacatca aggttatgat cttgcatcag gtatagaagt aaatgggcga
    13261 aaagatatag aaaaaatgac agcaattgta aatattgtga aaggagatag agacaatgaa
    13321 taaacaaata caaacagaag cagatgaatt aggtttcttt ggtgaatatg gagggcaata
    13381 tgttccagaa acattaatgc cagcaattat tgagttgaaa aaagcttata aagaggcaaa
    13441 agcagaccca gagtttcaaa gagaactgga atactattta tcagagtatg taggacgcgc
    13501 gacaccactt acatatgctg catcatatac tgaaagccta ggtggcgcta aaatatattt
    13561 gaaacgagag gatctaaatc atacaggcgc ccataaaatt aataatgcgt taggtcaagc
    13621 gttgcttgct aaaagaatgg gcaagaagaa gcttgttgct gaaactggtg cgggtcaaca
    13681 tggtgtagct agtgctacgg ttgctgcatt atttgatatg gaacttgttg tctttatggg
    13741 aagtgaagat attaaaagac aacaacttaa tgtatttaga ctggaattac ttggtgcaaa
    13801 ggttgtggca gttgaagatg gtcaagggac tttatcggat gcagttaata aagcattgca
    13861 atattgggta agtcatgtag atgatacaca ttatttatta ggttctgcat taggtccaga
    13921 cccgttccca acgattgtta gagattttca gagtgtgatt ggtaaagaaa taaaatcaca
    13981 gatattgaag aaagaaggtc gacttccgga tgcaattgta gcatgtatcg gtggtggctc
    14041 aaatgcaatc ggtacatttt atccatttat taaagatgat gttgcattat acggtgttga
    14101 agccgcaggt caaggcgatg atactgataa acatgcactt gcaattggca aaggatcacc
    14161 tggcgtatta catggtacta aaatgtattt aattcaagat gaagatgggc aagtgcaact
    14221 agcacattct atttcagcag gacttgatta tcctggtatt ggaccagaac attcttatta
    14281 ccacgacatt ggtagagtaa cttttgaaaa tgctagtgat acacaagcaa tgaatgcttt
    14341 aatcaacttt acaaaacatg aaggtattat acctgcaatt gaaagtgcac atgcactgag
    14401 ttatgttgaa agactagcgc ctacgatgtc gaaagaagat attattgtag taactatttc
    14461 tggacgtggc gataaagata tggaaacaat tagacaatat atggtagagc gaggtcttgc
    14521 aaatgactaa attatttata ccttatatta tgggcaataa agatttgatt gaaaatgcaa
    14581 cattgttgag tgaaaatggt gcagatataa ttgaaattgg agtacctttc tctgatccgg
    14641 ttgctgatgg tccagttatc atggaagcag gtcaacaagc gattaaacaa ggcatcacga
    14701 tagattatat tttcaatcaa ttagaaaaac atggtgatca aattaagtgt aactatgtat
    14761 taatgacgta ttataatatt atttgtcatt atggagaaca agcgtttttt gaaaaatgtc
    14821 gagatactgg tgtctacggc ttaattattc ctgatttacc atatgaatta tcgcagcgtt
    14881 taaaacaaca atttagtcac tatggcgtca aaatcatatc gttagttgtg atgactactg
    14941 atgacaaacg tataaaagat atcgtatccc atgcggaagg ctttatttat actgtgacga
    15001 tgaatgcgac aacagggcaa aacggtgcgt ttcatccaga attaaaacga aaaattgagt
    15061 caattaaagc gatagccaat gtgccagttg tcgcaggatt tggtataaga acaccacaac
    15121 atgttgcaga tataaaagag gttgcagatg gcattgtcat tggtagcgaa atcgttaagc
    15181 gatttaaatc taacacgcgt gaggaaatca ttaaatattt acaatctatc caacaaacat
    15241 tgaataatta agtttacttg atttaaaaaa attaggcgaa tactgtttga aaaagtgaaa
    15301 aacggtgaat tataaaattg aatagaaatg atacaaaagt aatatgagca aacccaaacg
    15361 ttcatattac tttttttgaa attgtattca aaaatctaaa tattactata aaagtatacg
    15421 caattaaagc gtttatgttt tagttttaac attaactatt gtatacttat ttagactaga
    15481 tttattattt ttgacatttg cagaggggaa atagaaaaac tgcaaatacg gaaatgaaat
    15541 taattaacga gagacaaata ggagtaatga taatgaagtt tacaaattta acagctaaag
    15601 agtttggtgc ctttacagat agcatgccat acagtcattt cacgcaaact gttggccact
    15661 atgagttaaa gcttgctgaa ggttatgaaa cacatttagt gggaataaaa aacaataata
    15721 acgaggtcat tgcagcttgc ttacttactg ctgtacctgt tatgaaagtg ttcaagtatt
    15781 tttattcaaa tcgcggtcca gtgattgatt atgaaaatca agaactcgta cactttttct
    15841 ttaatgaatt atcaaaatat gttaaaaaac atcgttgtct atacctacat atcgatccat
    15901 atttaccata tcaatacttg aatcatgatg gcgagattac aggtaatgct ggtaatgatt
    15961 ggttctttga taaaatgagt aacttaggat ttgaacatac tggattccat aaaggatttg
    16021 atcctgtgct acaaattcgt tatcactcag tgttagattt aaaagataaa acagcagatg
    16081 acatcattaa aaatatggat ggacttagaa aaagaaacac gaaaaaagtt aaaaagaatg
    16141 gtgttaaagt aagattttta tctgaagaag aactaccaat ttttagatca tttatggaag
    16201 atacgtcaga atcaaaagct tttgctgatc gtgatgacaa attttactac aatcgcttaa
    16261 aatattacaa agaccgtgtg ttagtacctt tagcgtatat caactttgat gaatatatta
    16321 aagaactaaa cgaagagcgt gatattttaa ataaagattt aaataaagcg ttaaaggata
    16381 ttgaaaaacg tcctgaaaat aaaaaagcac ataacaagcg agataactta caacaacaac
    16441 ttgatgcaaa tgagcaaaag attgaagaag gtaaacgtct acaagaagaa catggtaatg
    16501 aattacctat ctctgctggt ttcttcttta tcaatccatt tgaagttgtt tattatgctg
    16561 gtggtacatc aaatgcattc cgtcattttg ccggaagtta tgcagtgcaa tgggaaatga
    16621 ttaattatgc attaaatcat ggcattgacc gttataattt ctatggtgtt agtggtaaat
    16681 ttacagaaga tgctgaagat gctggtgtag ttaaattcaa aaaaggttac aatgctgaaa
    16741 ttattgaata tgttggtgac tttattaaac caattaataa acctgtttac gcagcatata
    16801 ccgtacttaa aaaagttaaa gacagaattt tttaggaagg gaattatcaa aacatgaaat
    16861 ttacagagtt aactgttacc gaatttgaca actttgtaca aaatccatca ttggaaagtc
    16921 attatttcca agtaaaagaa aatatagtta cccgtgagaa tgatggcttt gaagtagttt
    16981 tattaggtat taaagacgac aataacaaag taattgcagc aagccttttc tctaaaattc
    17041 ctactatggg aagttatgtt tactattcga atcgtggtcc agtaatggat ttttcagatt
    17101 taggattagt tgattattat ttaaaagagt tagataaata tttacagcaa catcaatgtt
    17161 tatatgttaa attagatccg tattggttat atcatctata tgataaagat atcgtgccat
    17221 ttgaaggtcg cgagaaaaat gatgccctag taaacttgtt taaatcacat ggttacgagc
    17281 atcatggctt tacaactgag tatgatacat cgagccaagt acgatggatg ggcgtattaa
    17341 accttgaagg taaaacaccc gaaacattga aaaagacatt tgatagtcaa cgtaaacgta
    17401 atattaataa agcgataaac tatggtgtta aagtcagatt ccttgaacgt gatgagttca
    17461 atcttttctt agatttatat cgtgaaactg aagagcgtgc tggatttgta tcaaaaacag
    17521 atgattattt ttataacttt attgacacat atggagataa agtattagta ccattagcat
    17581 atattgacct tgatgaatat gtgttaaagt tgcaacagga attgaatgac aaagaaaatc
    17641 gtcgtgatca aatgatggcg aaagaaaaca aatcagataa gcaaatgaag aaaattgcag
    17701 aattagataa gcaaattgat catgatcagc atgaattatt gaatgcaagt gaattgagca
    17761 aaacggacgg cccaattcta aaccttgctt ctggcgttta ttttgcaaat gcatatgaag
    17821 tgaattattt ctctggtggt tcatcagaaa aatataatca atttatggga ccatacatga
    17881 tgcattggtt tatgattaac tattgcttcg ataatggcta tgatcgttat aatttctatg
    17941 gtttatcagg tgattttacg gaaaacagtg aagattatgg cgtataccgc tttaaacgtg
    18001 gatttaatgt acaaatcgaa gaattaatag gggatttcta taaaccaatt cataaagtga
    18061 aatattggtt gttcacaaca ttggataaat tacgtaaaaa attaaagaaa tagatgtaaa
    18121 tgatgttagg gcttatagtt attgatacta taggctcttt tttatatgtt ttttaataaa
    18181 gcctcgaata atgatatatt aaatgacaag tttaacaagt gattgcaata aatataaata
    18241 tgaattgcac aacctatttg taaatgattg agttgagtat actttttaat tcattcaata
    18301 tgactcaaaa caagccaata tgtattatat ttttggtgaa taaaccttag cataacaatt
    18361 tatgtctgat tatatatgtg tgtgtttgca agtgtgtgat atcaaatgtc atatttgtct
    18421 aattgtaatt tataaaatgc atgagcaatg attgctgtaa caaattctag caaagtattc
    18481 agtttgtatt ttagaaataa aaatgatgat agatgtatga tcgatataaa agatttaata
    18541 tgcaccaaaa cgtgagtaat tggtttaatg aaacataaca tgaatgtaaa tttagcttac
    18601 tgacataaac actacacaaa catacagtta tataagtccc cttatggaat ttcacattct
    18661 agtttacata atatatatta taggaagtta tatgtgtgta acgcaaaagg taccctacat
    18721 cataatcatt atctaatatc gtcacataac ttacttatgc tataatcatg gtattatatt
    18781 gtttggagtg atttgatgag atttgtcttt gatattgatg gtacgctttg ttttgacggc
    18841 cgattaattg accagactat tattgataca ttgttacaat tacaacatga tggtcatgaa
    18901 cttatatttg catcagcacg tccgattcgt gatttgttgc cagttttacc atcagtattt
    18961 catcagcaca cattaattgg cgcaaatggt gctatgattt cacagcaatc aaagatttct
    19021 gttatcaaac caattcatac tgatacatat catcatatct tcaaaataat tcaaaagtat
    19081 gagttagatt atattattga tgatgattgg aattatgctg cacaacttga cgctgagaac
    19141 gcgatttttg agcgtttaga tccacataag ctggccagtt gtattgatgt tgcaaatatc
    19201 gacacgccaa tcaagattat tttattaaat atagacccgg cacaaattac aactatatta
    19261 gacgagctag ataaatacca tcaagaattg gaaatgattc accattcaaa tgagtataac
    19321 attgatataa cagcgcaaaa tattaacaaa tatactgcat tacaatatat atttgatgca
    19381 gatgttaaat atatagcatt tggtaatgac cacaatgata ttgtcatgtt acaacatgct
    19441 agtagtggct atattatagg accatcagaa gcatacacac acgcaatatt gaaacttgat
    19501 aaaatcaaac acatcaataa taatgcacaa gctatttgca aagtcttaaa atcatataaa
    19561 taaaaacacc cctatcaaat gataatcatt atcaatcgat aggggctatt ttaataaaat
    19621 tcgtcctcga acatttcttc ctcttcatct aatccaaata attctgccat ttctccatgt
    19681 tcaattaaca tgtttaaata tgcatcgcgg agttcttctt cactcatatc attaatcatt
    19741 tctttaagac tatcaatcca catatttctg cgtaattgat agtcttcttc aacttcgttt
    19801 aacatcatta tatgtttatt tgctgcttct ggactagctg taaagagtaa tgcaatcata
    19861 tgtttacata tcactcgtct tccatcagca tgaggacaat tacatatgga ttttctagga
    19921 tgttccatat caatataaca acgatatact ttgttgccac tgccctttac ttcagcctca
    19981 tgctgcgttt ctgaaaatga ttttaagtta atgacgcatt cactttgata ataattaaag
    20041 cctctttcta tagaacgaat acttgcaata tcaagtaatc cc
//
LOCUS       DAXWFK010000036        18734 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.036, whole genome shotgun sequence.
ACCESSION   DAXWFK010000036 DAXWFK010000000
VERSION     DAXWFK010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 18734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18734
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.036"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..97
                     /locus_tag="ACGEXS_000989"
     CDS             <1..97
                     /locus_tag="ACGEXS_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012010.1"
                     /translation="AIAKFKPFKNRIKNNPQKTNPFLKLHENKKS"
     gene            358..630
                     /locus_tag="ACGEXS_000990"
     CDS             358..630
                     /locus_tag="ACGEXS_000990"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFF8012011.1"
                     /translation="MTKERRTFSSEFKLQMVRLYKNGKLKNEIIRKYDLKPSIISNSI
                     KQHQNTGPFNHQDNLKSDEKELIKLRKEVQHLKMEHDVLKQILKLI"
     gene            1029..1733
                     /locus_tag="ACGEXS_000991"
     CDS             1029..1733
                     /locus_tag="ACGEXS_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFF8012012.1"
                     /translation="MSKNINQANSKLNTSNKKLKQAYSKSDSKNMKVIYMPHWLEFYS
                     IAIHSDVTSNKKRYYKSYSRGTVVYVKLGSNIGSEFSGNHFCVILDNKDNKGKETVTI
                     VPLSSKGNKNYLKLNESVLNLTATDLKKQIIDISSKVQALAQKYREIDIKLSTEDKKL
                     LSNLNQKANELYRVIGVYSKHKGKDTYVNISAITTISKRRISKINDSDPTGTIIISED
                     DMNEIEKQLKIKFFSN"
     gene            2126..2662
                     /locus_tag="ACGEXS_000992"
     CDS             2126..2662
                     /locus_tag="ACGEXS_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012013.1"
                     /translation="MHNNNEFNKKLKDFIGSDKRMALVKGYVNEYKLTTVLRALNNSD
                     YTKGTIHTRSIGNLKQIVDNRIIPKNITQNTIFKLSNLTLEVNLYERNTIYHGDFAIY
                     YPVESALIIETDTPRLINHINNNPVQKIFIITTNDWSFNTLELEKIVDETIIYDLKQE
                     DPKQYEILYKNKHGKLPY"
     gene            complement(2775..4316)
                     /gene="guaA"
                     /locus_tag="ACGEXS_000993"
     CDS             complement(2775..4316)
                     /gene="guaA"
                     /locus_tag="ACGEXS_000993"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019166941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFF8012014.1"
                     /translation="MEMAKEQELILVLDFGSQYNQLITRRIREMGVYSELHDHEISIE
                     EIKKMNPKGIILSGGPNSVYEEGSFTIDPEIYNLGIPVLGICYGMQLTTKLLGGKVER
                     ANEREYGKAIINAKSDELFAGLPAEQTVWMSHSDKVIEIPEGFEVIADSPSTDYAAIE
                     DKKRRIYGVQFHPEVRHTEYGNDLLNNFVRRVCDCRGQWTMENFIEIEIEKIRQRVGD
                     RRVLCAMSGGVDSSVVAVLLHKAIGDQLTCIFVDHGLLRKGEGDMVMEQFGEGFNMNI
                     IRVNAKDRFMNKLKGVSDPEQKRKIIGNEFVYVFDDEASKLKGVDFLAQGTLYTDVIE
                     SGTKTAQTIKSHHNVGGLPEDMEFELIEPINTLFKDEVRKLGIELGIPEHLVWRQPFP
                     GPGLGIRVLGEITEDKLEIVRESDAILRQVIREEGLEREIWQYFTVLPNIQSVGVMGD
                     YRTYDHTVGIRAVTSIDGMTSDFARIDWEVLQKISSRIVNEVDHVNRVVYDITSKPPS
                     TIEWE"
     gene            complement(4341..5807)
                     /gene="guaB"
                     /locus_tag="ACGEXS_000994"
     CDS             complement(4341..5807)
                     /gene="guaB"
                     /locus_tag="ACGEXS_000994"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002955225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFF8012015.1"
                     /translation="MWESKFAKESLTFDDVLLIPAQSDILPKDVDLSVQLSDKVKLNI
                     PVISAGMDTVTESKMAIAMARQGGLGVIHKNMGVEEQADEVQKVKRSENGVISNPFFL
                     TPEESVYEAEALMGKYRISGVPIVDNKEDRNLVGILTNRDLRFIEDFSIKIVDVMTQE
                     NLITAPVNTTLEEAEKILQKHKIEKLPLVKDGRLEGLITIKDIEKVIEFPNAAKDEHG
                     RLLVAAAIGISKDTDIRAQKLVEAGVDVLVIDTAHGHSKGVIDQVKHIKKTYPEITLV
                     AGNVATAEATKDLFEAGADIVKVGIGPGSICTTRVVAGVGVPQITAIYDCATEARKHG
                     KAIIADGGIKFSGDIIKALAAGGHAVMLGSLLAGTEESPGATEIFQGRQYKVYRGMGS
                     LGAMEKGSNDRYFQEDKAPKKFVPEGIEGRTAYKGALQDTIYQLMGGVRAGMGYTGSH
                     DLRELREEAQFTRMGPAGLAESHPHNIQITKESPNYSF"
     gene            complement(5845..7113)
                     /gene="pbuX"
                     /locus_tag="ACGEXS_000995"
     CDS             complement(5845..7113)
                     /gene="pbuX"
                     /locus_tag="ACGEXS_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine permease PbuX"
                     /protein_id="HFF8012016.1"
                     /translation="MKNLILSVQHLLAMYAGAILVPIIVGTSLKFTPEQIAYLVTVDI
                     FMCGVATFLQANKVTGTGLPIVLGCTFTAVAPMILIGQTKGIDVLYGSLFLSGILVII
                     IAPFFSHLVKFFPPVVTGSVVTIIGINLMPVAMNYLAGGQGAKDYGDVKNILLGLMTL
                     IIILLLQRFTTGFIKSIAILIGLVLGTIGAGLLGMVDINQVNHAGWLGIPVPFRFSGF
                     SFDVTSTLVFFIVAIVSLIESTGVYHALSEITGKKLERKDFRKGYTAEGLAIVLGSIF
                     NSFPYTAYSQNVGLVSLSGAKKNNVIYGMVVLLLICGCIPKLGALANIMPLPVLGGAM
                     IAMFGMVMAYGVSILGHIDFKNQNNLLIIAVSVGLGTGISAVPQAFKGLGEQFAWLTQ
                     NGIVLGAISAIILNFFFNGIKYKQTEENVK"
     gene            complement(7113..7691)
                     /gene="xpt"
                     /locus_tag="ACGEXS_000996"
     CDS             complement(7113..7691)
                     /gene="xpt"
                     /locus_tag="ACGEXS_000996"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFF8012017.1"
                     /translation="MELLGQKVKEDGVVIDEKILKVDGFLNHQIDAKLMNEVGRTFYE
                     QFKDKGITKILTIEASGIAPAIMAALHFDVPCLFAKKAKPSTLTDGYYETSIHSFTKN
                     KTSTVIVSKEFLSEEDTVLIIDDFLANGDASLGLYDIAQQANAKTAGIGIVVEKSFQN
                     GHQRLEEAGLTVSSLCKVASLEGNKVTLVGEE"
     regulatory      complement(7922..8024)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            8204..8611
                     /locus_tag="ACGEXS_000997"
     CDS             8204..8611
                     /locus_tag="ACGEXS_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFF8012018.1"
                     /translation="MADITVVNDTGELYNVINQKKSEGYLESELTIISKSKLHLNDLH
                     DSEISLISTSGTFSDRMTKLLTGEDGEHAVLSRYNLAPDELEKYKQLILDDKMLVVAV
                     CDKSSHQEVHENNSAYEEIDITHFAEASKGPKA"
     gene            8754..9416
                     /locus_tag="ACGEXS_000998"
     CDS             8754..9416
                     /locus_tag="ACGEXS_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012019.1"
                     /translation="MEFKTIQSVKDPHYNEALKLYDTKLNLSLTEDKRIIKRSLENNK
                     TENDYVFIVGLENDVVVSLATAHYEATTNSAFLIYLFAKDLPNHDELMTQTLHQIEHE
                     LNLLSNLVHSRSINFLMMEVPKEPADLTQDNGLIIEHRRQFLYEHQFERQTEIDYIHP
                     TNDGHTKKVDLFLKKNIKLTKDIYGTSVKSNYILKYVFANGIAREIIYALLEEMDLRK
                     PF"
     gene            9534..10490
                     /locus_tag="ACGEXS_000999"
     CDS             9534..10490
                     /locus_tag="ACGEXS_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012020.1"
                     /translation="MLTKEFAQRVELSEKQVRKIVQHLEERGYQLSKTEYRGREATDF
                     KEEDIELFKDIADKVKQTNSYDLAFDELEKEKDFLQVIVKNDDKNLPTNQNVAQLVED
                     LRLEIQKMREERHLLGQMMNQVHQQQQELKELQNQLTSKIDSNSESLKAIQTSQEAIQ
                     EAQASQAKALAESTNKVEKNAVTEDKADSKDSKVAGVNTSTDAKTDTKAENAGDGTAT
                     KVDKEDQISATEAIEKASVEQSKNDNAAETSNKEATVDAEAQHDAEQQVAEAHAEASK
                     QATSNDSLEAKAENDSTASQSEMSEPKPQEEKKGFFARLFNL"
     gene            complement(11281..11382)
                     /locus_tag="ACGEXS_001000"
     CDS             complement(11281..11382)
                     /locus_tag="ACGEXS_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012021.1"
                     /translation="MQLNVNMMKFDRWHKGICKQIVLNSVVIGGKGK"
     gene            11467..12855
                     /locus_tag="ACGEXS_001001"
     CDS             11467..12855
                     /locus_tag="ACGEXS_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFF8012022.1"
                     /translation="MNAFLTLINIIVLVIFIVILHMMARKHISFAKRVFTALGIGIVF
                     GVLLHLIYGTHSNVITSTSDWFNIVGQGYVALLQMIVMPLIFISIVAAFTKIQIGEKF
                     AKIGSLIFIFLIGTVTIAAIVGVVYALVFGLDASTINLGNAEQARGSEIAKQAKDLTA
                     HTLPQQILELLPKNPFLDFTGQRATSTIAVVIFASFIGFAYLRVARKQPDHGELLKRA
                     IDAIYSLVMAIVTFVLRLTPYGVLAIMANTLSTSDFGAIWTLGKFLIASYAALITMYI
                     IHLIILSLLGISPIRYVKKTLEVLIFAFTSRSSAGALPLNVQTQTRRLGVPEGIANFA
                     ATFGLSIGQNGCAGIYPAMLAIMVAPVANVEIDLQFIVTLIAVVIISSFGVAGVGGGA
                     TFASILVLSTLNLPVALAGVLISVEPLIDMGRTALNVNDSMLAGTGTAKLTKHWDKDT
                     FDSNDNAALTSH"
     gene            complement(12936..13691)
                     /gene="nfsA"
                     /locus_tag="ACGEXS_001002"
     CDS             complement(12936..13691)
                     /gene="nfsA"
                     /locus_tag="ACGEXS_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NADPH nitroreductase"
                     /protein_id="HFF8012023.1"
                     /translation="MSEHVYNLVKKHHSVRKFKNKPLSEDVVKKLVEAGQSASTSSFL
                     QAYSIIGIDDEKIKENLREVSGQPYVVENGYLFVFVIDYYRHHLVDQHAETDMENAYG
                     STEGLLVGAIDAALVAENIAVTAEDMGYGIVFLGSLRNDVERVREILDLPDYVFPVFG
                     MAVGEPADDENGAAKPRLPFDHVFHHNKYHADKETQYAQMADYDQTISEYYDQRTNGN
                     RKETWSQQIEMFLGNKARLDMLEQLQKSGLIQR"
     gene            14183..14752
                     /gene="ahpC"
                     /locus_tag="ACGEXS_001003"
     CDS             14183..14752
                     /gene="ahpC"
                     /locus_tag="ACGEXS_001003"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFF8012024.1"
                     /translation="MSLINKEILPFTAQAFDPKKDQFKEVTQEDLKGSWSVVCFYPAD
                     FSFVCPTELEDLQNQYEELQKLGVNVFSVSTDTHFVHKAWHDHSDAISKITYTMIGDP
                     SQTITRNFDVLDEATGLAQRGTFIIDPDGVVQASEINADGIGRDASTLAHKIKAAQYV
                     RKNPGEVCPAKWEEGAKTLQPGLDLVGKI"
     gene            14768..16291
                     /gene="ahpF"
                     /locus_tag="ACGEXS_001004"
     CDS             14768..16291
                     /gene="ahpF"
                     /locus_tag="ACGEXS_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFF8012025.1"
                     /translation="MLNADLKQQLKQLLELMEGNVEFVASLGSDDKSKELKDLLTEIT
                     DMSPRLSLSEKSLKRTPSFSVNRPGEETGVTFAGIPLGHEFNSLVLAILQVSGRAPKE
                     KQSIIDQIKKLEGSFHFETFISLTCQKCPDVVQALNLMSVINPNITHSMIDGAVFREE
                     SENIMAVPAVFLNGEEFGNGRMTIQDILSKLGSTADASEFENKEPYDVLIVGGGPASG
                     SAAIYTARKGLRTGIVADRIGGQVNDTAGIENFITVKETTGSEFSSNLAAHIDQYDID
                     AMTGIRATDIEKTDEAIKVTLENGAVLESKTVIIATGAGWRKLNIPGEEQLINKGVAF
                     CPHCDGPLFENKDVAVIGGGNSGVEAAIDLAGIVNHVTLFEFASELKADNVLQDRLRS
                     LSNVDIKTNAKTTEVVGEDHVTGIRYEDMNTGEEHLLNLDGIFVQIGLLPNTSWLNDA
                     VELNERGEIVIDRNNNTNVPGIFAAGDVTDQKNKQIIISMGAGANAALNAFDYIIRN"
     gene            16421..16663
                     /locus_tag="ACGEXS_001005"
                     /pseudo
     CDS             16421..16663
                     /locus_tag="ACGEXS_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            16736..17362
                     /locus_tag="ACGEXS_001006"
     CDS             16736..17362
                     /locus_tag="ACGEXS_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDxxF motif lipoprotein"
                     /protein_id="HFF8012026.1"
                     /translation="MKKRLLLSTFLASTLILTGCASDQSDNEDHHTSTGIHAPKSAKK
                     LETKDIFNSDKKNSDISDAEMKQAIEKYLSVNSDILDNKYIMQHKLDKQIDSQTKVTE
                     KQAETLSHLSNLAVKNDLHFKKFVTENNIPKEYKKPVELMMNYFKALNSTIANVDEDI
                     EKLSYQPQNKINVVDVPTKYAGDVNKKQQDKIKDFLKSKGIKSDVIDK"
     gene            17617..18000
                     /locus_tag="ACGEXS_001007"
     CDS             17617..18000
                     /locus_tag="ACGEXS_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012027.1"
                     /translation="MKVRITFIILAILSTIVCLFLAAMHPTGPNTVTFEQPYLFTLNI
                     IIMVLVALPSLVLAIYDYMSFRISSAILQFLGAISWFFLSLILSLTQFTPFTLASFIT
                     SIILFTSTIITLAIGGKSVEKNDSP"
     gene            complement(18066..18647)
                     /locus_tag="ACGEXS_001008"
     CDS             complement(18066..18647)
                     /locus_tag="ACGEXS_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFF8012028.1"
                     /translation="MTIYLVRHGESKSNYDNKHFRSYFCGQLDVPLTDTGTKSADDLC
                     DYFKEKQIKHVYVSDLLRTQQTFEHIFPYDIASTTTPLLRERSLGVFEGEYKDEISAN
                     PKYEKYFNDPNFKDFRHSFSQKAPEGESYEDVYQRVEHFMNHVVNEDTQKDDIVIVAH
                     QVVIRCLMVYFNKVSRAEAVDLKVENCKPYIIE"
BASE COUNT         6427 a         3424 c         2905 g         5978 t
ORIGIN      
        1 tgcaatcgca aaattcaaac catttaagaa cagaattaaa aataacccac aaaaaacaaa
       61 tccatttctt aaattacatg aaaacaaaaa atcttaatca ctttatttat agcatttcta
      121 atctcagaaa tgctataaag gacaacgccc tctttcataa ctatataagt caagaaacca
      181 agacatttca aacatcttta aacccttcaa tagcacattc cctaaaacat aaagcaataa
      241 caatgaacga tcaaaattcc ttacgtactt atgatgtgtc agtgaatcgt ctacacttaa
      301 ttgaacaaat tctatgagaa tagatattgt taaattaaga aagtagtcaa ttttattatg
      361 acaaaagaaa gaagaacatt tagttcagag tttaagttac aaatggttag attatataaa
      421 aatggtaagc ttaagaatga aattatacga aagtatgatt taaaaccctc aattatctca
      481 aattcgataa aacaacacca aaatactgga cccttcaatc atcaagataa tttaaaaagt
      541 gatgaaaaag agttaataaa attacgcaaa gaagttcaac atttaaaaat ggaacatgat
      601 gttttaaagc aaattttaaa gttgatttag aaactgaact ttttaaagat acattacttg
      661 aagattctaa tttatctgat tatataaaga accaacttcc aacgatatgg agagactaag
      721 aaatagttta aatttattta atttcaataa aaactcataa taatatttaa gtaagttaga
      781 ttgcttttag cattaggaat tcgcttataa ttagatgttc aatatagact tttttacaca
      841 tacatgaact ataaaaagcg aataaatagc aggtcattgc gaaatttgct aaatacaaaa
      901 ttgatgagtt ccacctaata aaaattgttg gttttatagt aacatctatt ttcgtaccat
      961 tcatgttaat ttcagtgcta ataatgctga tataaagaag aataaattga ttaggagaac
     1021 actatttaat gtctaaaaat atcaatcaag caaatagtaa attaaatacg tcaaacaaaa
     1081 aactaaaaca agcttattca aaatccgaca gtaaaaacat gaaagttata tacatgcctc
     1141 attggctaga gttttattca attgctatac atagcgatgt tacaagcaat aaaaaaagat
     1201 attataaatc atatagtaga ggaacagtgg tatatgtaaa actagggagt aatattggaa
     1261 gtgagttttc tggtaatcat ttttgtgtaa tattagataa caaagacaat aaaggaaaag
     1321 aaacagttac aatagttcca ctttcttcca aaggtaataa aaattatttg aagttaaacg
     1381 aatcggtgct aaatctaaca gcaacagatt taaaaaaaca gattatcgat atcagtagta
     1441 aagtacaagc attagcccaa aaatatagag aaattgacat taaacttagc acagaagata
     1501 aaaagctttt gtctaactta aatcaaaaag ctaatgaact gtatagagtg ataggtgttt
     1561 attccaagca caaagggaaa gatacatacg ttaatatatc agcaattaca acaataagca
     1621 aaagacgtat aagcaaaata aatgattctg atcctactgg aacgataata atttcagaag
     1681 atgacatgaa tgaaatcgaa aagcaattaa agataaagtt cttttctaat tgaattgtaa
     1741 tattacatct gatataattg atttacaaat tcccggtaac cattccggct tcattctctt
     1801 gaagaatgac atattctcag cgttttagct gaaggtcaga tgatacgtca tctggcctct
     1861 ttttttatat aaatgccctt taagtgaaat atacaacaat ctgcaatcaa tattagacat
     1921 cacaaaacac acaataaatt acacaatgtg ttataagcca catgtcacta ttatcgcagc
     1981 tagctgcgtc actcaaaacg aatacggaac gcgttggtta tttcaatcat aatattactc
     2041 tgcaaataca cagccctgta atcgagtaaa tggatagaga tttgaacaaa tgaaaacaat
     2101 caactaatgg aaaggataaa atattatgca caacaataat gaattcaaca aaaaactgaa
     2161 agattttata ggtagcgata aacggatggc tttagtaaag ggttatgtta acgagtataa
     2221 attaacaact gttttaagag ctttaaataa ttccgattac acaaaaggga cgatacacac
     2281 tcgttcaata ggcaacctaa aacaaatagt agataatcga attattccta aaaatataac
     2341 tcaaaacaca atcttcaaat tgagtaattt aaccttagag gttaacctct atgaaagaaa
     2401 cacaatttat cacggagact ttgctattta ctatcctgta gaatctgctc taataataga
     2461 aacagatact cctaggctaa ttaatcatat aaataacaac ccagtccaaa aaatatttat
     2521 aataacaaca aatgactgga gctttaatac acttgaatta gaaaaaatag tagacgaaac
     2581 tataatttat gatttaaaac aagaagatcc taaacaatat gaaattttat ataaaaataa
     2641 acacggaaaa ttgccttatt gatgaattaa aacgcaaact tagaacacct tacccctttt
     2701 ccgtccttat tgtaattttc caactactca aattaaaaac cacccgtgca aacaggtggt
     2761 tttattatat ataattattc ccactcaatt gtgcttggtg gttttgatgt aatgtcatag
     2821 actacgcggt tgacgtgatc tacttcgttt acgatacgac tagaaatctt ttgtaagact
     2881 tcccaatcga tgcgtgcgaa gtcacttgtc ataccgtcga tagatgttac tgcacgaata
     2941 cctactgtgt gatcatacgt acggtagtct cccataacac ctactgattg aatgtttggt
     3001 aacactgtga agtattgcca aatttctctt tcaagacctt cttctctaat cacttggcgt
     3061 aaaatcgcgt ctgattctct aacgatttct agtttatctt cagtaatttc tccaagtaca
     3121 cgaataccaa gaccaggtcc tgggaatggt tgtctccata ctaaatgttc tggaatacct
     3181 aactcaatac ctaatttacg tacttcatct ttaaacaatg tattgattgg ttcgattaat
     3241 tcgaattcca tgtcttctgg taatccacca acattgtggt gtgatttgat tgtttgtgct
     3301 gttttagtac ctgattcgat gacgtctgta tatagtgttc cttgcgcaag gaagtctaca
     3361 cctttcagtt ttgatgcttc atcatcaaat acgtatacaa attcattacc aatgatttta
     3421 cgtttttgtt caggatctga aacacctttt aatttattca taaagcgatc tttcgcatta
     3481 acacgaataa tattcatgtt gaaaccttca ccgaattgct ccataaccat gtcgccttca
     3541 cctttacgaa gtaagccatg gtctacaaag atacatgtta gttgatcacc tattgcttta
     3601 tgcaatagta cagctacaac agatgaatct acgccgccac tcatcgcaca taatacacga
     3661 cggtctccta cgcgttgacg aatcttttca atttcgattt cgataaagtt ttccattgtc
     3721 cattgacctc tacaatcaca aacacgacgg acaaaattat ttaataaatc attaccatat
     3781 tctgtatgac gtacttctgg atggaattga acaccataaa tgcgacgttt cttatcttcg
     3841 attgctgcat agtctgtgct tgggctatca gcgataactt caaagccttc tggaatttca
     3901 ataactttat cagaatgact catccaaaca gtttgttctg ctggtaagcc agcgaataac
     3961 tcatctgact tcgcattaat gattgcttta ccgtattcac gttcattggc acgttcaact
     4021 ttaccaccta ataatttagt agttaattgc atgccgtaac aaataccaag tactggaatt
     4081 cctaaattat atatttccgg atcaattgta aatgaacctt cttcataaac tgaatttgga
     4141 ccacctgata agataatacc ttttggattc attttcttaa tttcttcaat tgaaatttca
     4201 tgatcgtgta attcactata aacgcccatt tcacgaattc ggcgtgtaat taattggttg
     4261 tattggctac caaagtctaa gacaaggatt aactcttgtt cttttgccat ttccatattt
     4321 gtcgttctcc tttatcttaa ttagaatgag tagttcggtg attctttcgt aatttgaata
     4381 ttatgtggat ggctttctgc taaaccagca ggacccatac gtgtaaattg tgcttcttcg
     4441 cgtaattctc ttaaatcgtg tgaaccagta taacccatac cagcacgcac accgcccatt
     4501 aattggtaaa ttgtatcttg taacgcacct ttataagccg tacgtccttc gataccttca
     4561 ggaacaaatt tcttaggcgc tttgtcctct tggaagtaac ggtcgtttga acctttttcc
     4621 atcgcaccta aagagcccat accacggtat actttatatt gtctaccttg gaaaatttct
     4681 gttgcgcctg ggctttcttc agtacctgct aataagctac ctaacataac cgcatgtcca
     4741 ccagcagcta atgctttaat gatatctcct gagaatttaa taccaccatc agcaatgata
     4801 gctttaccat gtttgcgtgc ttcagttgca caatcataaa ttgctgtaat ttgtggtaca
     4861 ccaacacctg ctacaacacg cgtcgtacaa attgaacctg ggccaatacc aactttaaca
     4921 atatctgcac ccgcttcaaa taaatctttt gttgcttctg cagttgctac gttacctgct
     4981 actaatgtga tttctgggta agtcttctta atatgtttca cttgatcgat aacaccttta
     5041 gagtgaccat gtgctgtatc gataactaag acatccacac ctgcttcgac taatttttga
     5101 gcacgaatat cagtatcttt tgaaatacca attgctgcgg ctacaagtag acgaccatgt
     5161 tcatcttttg ctgcattagg gaattcgata actttttcaa tatctttaat agtaataaga
     5221 ccttctagac gtccgtcttt aactaatggt aacttttcaa tcttatgttt ttggagaatt
     5281 ttttctgctt cttcaagtgt tgtattcact ggagctgtaa ttaaattttc ttgcgtcatt
     5341 acatctacaa ttttaatcga gaagtcttca ataaaacgta agtcacggtt tgttaaaata
     5401 cctactaagt tgcgatcttc tttattatca acaattggta cacctgaaat acggtattta
     5461 cccattaatg cttctgcttc ataaacgctt tcttctggcg ttaagaaaaa tgggtttgaa
     5521 atgacaccat tttctgagcg ttttactttt tgaacttcgt ccgcttgttc ttcaacgccc
     5581 atatttttat gaataacacc taaaccacct tgacgagcca tagcaatcgc cattttagat
     5641 tcagttacag tatccatacc agcagaaata actggaatat ttaatttaac tttgtctgat
     5701 aattgtacgc ttaaatcaac gtctttcggt aaaatatcag attgtgctgg aattaataac
     5761 acatcatcaa acgttaatga ttcttttgca aatttacttt cccacattaa aaacagcctc
     5821 catttttcaa attaattagt tatattattt cacattttct tccgtttgtt tatactttat
     5881 tccattaaaa aagaaattaa gaataattgc agagattgcg cctaaaacaa ttccgttttg
     5941 agtcaaccat gcaaattgtt cacctaaacc tttaaatgct tgtggtacag cgcttatacc
     6001 agtacctaat cctactgata cagcgataat taataaattg ttttgatttt taaaatcgat
     6061 atgtcctaat atactaacac catatgccat taccatgcca aacatagcta tcatcgcacc
     6121 gcctaacaca ggtagcggca tgatatttgc taatgcgcca agcttaggta tacaaccaca
     6181 tataagtaat aacacgacca tgccgtatat aacattgttt ttcttagcgc cggataaaga
     6241 aacaagtcct acattttgcg aataggctgt atacggaaat gaattgaata tagaacctaa
     6301 cactatcgct agaccttccg cagtataacc tttacgaaaa tcttttcttt ctaacttctt
     6361 accggtaatt tcacttaacg catgatagac acctgtcgac tcaattaaac taacgatagc
     6421 tacaataaag aacactaacg tcgatgtcac atcaaagcta aatccagaga atctaaacgg
     6481 cactgggatg cctaaccaac cggcatgatt gacttgatta atatcgacca tcccaagtaa
     6541 gccagcacct atcgttccta aaacgagtcc aattaatatg gcaatactct taataaatcc
     6601 agttgtgaat ctttgtaaaa gaagaataat gattaatgtc attaaaccta acaaaatgtt
     6661 cttaacatct ccatagtcct ttgcaccttg acctccagct aagtaattca ttgctactgg
     6721 cattaaattg ataccaatga tagtaacaac actacccgtt actactggtg ggaagaattt
     6781 tacaagatgt gaaaagaaag gcgcgatgat aataactaat atccctgata aaaatagcga
     6841 accataaagt acatctattc ctttcgtttg accaattaaa atcatgggcg caacagccgt
     6901 gaatgtacat ccaagaacga ttggtaatcc tgttcctgtt actttattgg cttgtaaaaa
     6961 tgtggcaacc ccacacataa atatatctac tgtaactaag taagcgattt gttcaggtgt
     7021 aaacttcaaa cttgtaccaa caatgattgg aactaagata gcacctgcgt acatagctaa
     7081 aagatgttga acacttagga ttaaattttt cattattctt ctcccaccaa tgtcactttg
     7141 tttccttcta gtgaagcaac cttgcagaga gaagaaactg ttaaacctgc ttcttctaaa
     7201 cgttgatgcc cattttggaa actcttttca acaacaatac caataccagc tgtcttagca
     7261 ttcgcttgct gtgcgatatc gtataatcct aatgaagcat caccatttgc taaaaagtca
     7321 tcgatgataa gtacagtatc ttcttctgat aaaaactctt ttgaaacaat gaccgtactt
     7381 gttttatttt tagtaaatga atgaatagat gtttcataat aaccatccgt caaagtgcta
     7441 ggttttgctt ttttcgcaaa taaacatggc acatcaaaat gcagtgcagc catgattgca
     7501 ggtgcgatac cggaagcttc aatggttaag attttagtaa tccctttatc tttaaattgc
     7561 tcgtaaaaag tgcgaccaac ttcattcatt aactttgcat caatttgatg atttaaaaat
     7621 ccatcgactt ttaaaatctt ctcatcaatg acaacgccgt cttcctttac tttttgtcct
     7681 agtaactcca ctcttaaaac ctcctcagat ttgtgtgaaa cattttgctc ttaaattggt
     7741 gctagataca aaaaaatccc caaactaaat aatagtttca gggtttatga gtgaacgaac
     7801 atgcataacg aatttgtcat gcaatcaatg taaaagaagt ttcatcaaat aaactgtgac
     7861 catcatataa aatgatataa atcacccacc atggttacaa tttaatggct gaagctactc
     7921 ctagtattgt gttgttactc atagtcatgt cgttcaaggc aacatggtag aaacttctaa
     7981 agccatattc tttagattat atgagtttat gtaaattatt taacgataat agcaaatttt
     8041 cggcattttt tcaataactg cttaggtaat cttttaatag ttttataaat gtacagagac
     8101 gtttaatgcg aatgaagact tataacttaa taatgtaagg gttttacttt ataataagaa
     8161 gggtaaaaga ttaaataaac aaatttttaa aggggcgaca actatggcag atattactgt
     8221 agtaaatgac actggcgaat tatacaacgt aatcaatcaa aagaaatctg aaggttattt
     8281 agaatcagag ctaacaatta ttagtaaaag taaattacac ctaaatgact tacacgactc
     8341 tgaaatctcc cttatctcaa caagtggaac atttagtgat agaatgacta aactgttaac
     8401 aggagaagat ggtgaacatg cagttttatc acgctacaac ttagcgcctg atgaattaga
     8461 aaaatataaa caacttattt tagacgataa aatgttagtt gtagcggttt gtgacaaatc
     8521 atcacatcaa gaagtgcatg aaaataattc agcttatgaa gagattgata ttacacattt
     8581 tgctgaagca tctaaaggtc ccaaagctta gttatcacac tgaattcaaa atgagattac
     8641 tgtactgtct aatttaagat agtacagttt tttataccat ttaaaagttt atgaacagtc
     8701 ttttacagca ggcgattatg ctataatatt atttaaattg gggaggtgtt cttatggaat
     8761 ttaaaaccat tcaatctgtc aaagatccac attacaacga agcacttaaa ctttacgata
     8821 ctaagttaaa tttatcttta actgaagata aacgtatcat caaacgctca ttagaaaaca
     8881 acaaaaccga aaacgattat gtctttatcg taggcttaga aaacgatgtt gttgtcagtc
     8941 tagcaactgc acattatgaa gccacaacca attcagcatt tttaatttat ttattcgcaa
     9001 aagacctacc caaccatgat gagttaatga cgcaaacatt acatcaaatt gaacatgaac
     9061 taaacttatt atcaaacctt gttcattcta gaagtattaa ctttttaatg atggaagtcc
     9121 ctaaagaacc tgcagattta acacaagata acggcctcat tattgaacac cgtagacagt
     9181 ttttatacga acatcaattt gaaagacaaa ctgaaattga ttacattcat ccaactaatg
     9241 atggtcacac taaaaaagtg gacttatttt taaagaaaaa tattaaactg acaaaggata
     9301 tctacggtac aagtgttaaa tcaaattata ttttgaagta tgtatttgcc aatggtatcg
     9361 cacgtgaaat tatttatgct ttattagaag aaatggattt acgtaaacca ttttaaacaa
     9421 acttgaaatc actaacaata acgtttaaac tatgtatgaa gcaagactga cgtttcactg
     9481 cccgtagaca aacgtcagta tacataaagt atatgcagaa aggactttaa agtatgttaa
     9541 ctaaagaatt tgcacaacgc gtagaactaa gtgagaagca agtacgtaaa attgttcaac
     9601 atttagaaga acgtggttac caactaagca agactgaata tcgtggtcgt gaagcaactg
     9661 atttcaaaga agaagatatc gagcttttca aagacattgc cgacaaagta aaacaaacaa
     9721 atagttatga tctagcgttt gatgaattag aaaaagaaaa agacttcctg caagtcattg
     9781 tcaaaaacga tgacaaaaac ttacctacta atcaaaatgt cgctcaacta gtagaagatt
     9841 tacgcctaga aatccagaaa atgcgcgaag aacgtcacct acttggtcaa atgatgaatc
     9901 aagtacatca gcaacaacaa gaattaaaag aacttcaaaa tcaacttaca tctaaaatcg
     9961 attcaaatag cgaatcctta aaagccatcc aaacatcaca agaggctatc caagaagcgc
    10021 aagcctctca agcaaaagca ttagctgaat caacaaataa agttgaaaag aatgctgtta
    10081 ctgaagataa agctgacagt aaagattcga aggttgctgg agtgaataca tcaacagacg
    10141 ctaagacgga tactaaagca gaaaatgcag gcgatggcac tgctactaaa gtagataaag
    10201 aggatcaaat ttctgcaact gaagcaattg aaaaagccag cgttgagcag tctaaaaatg
    10261 acaatgcagc tgagacttca aataaagaag ctacagtaga tgcagaagcg caacatgatg
    10321 ctgaacaaca agtagcagaa gcccatgcag aagcttctaa acaagctact tcgaacgatt
    10381 cattggaagc gaaagcagaa aatgacagca cagcttcaca gtcagaaatg tcagaaccaa
    10441 aacctcaaga agagaaaaaa ggtttcttcg cacgtttatt caacctgtaa acttgtgatt
    10501 tgaacacaaa tttaatactc aattcatata tagctttgat ttaaaaactg atttttataa
    10561 acctatagaa ctgtaattta ctttcaaatt taatactcaa ttcataaaac ctgaaacaag
    10621 ctgatatact ccattcaata tctactttga agagagcata tcaaactaat ttgtttcagg
    10681 ttttttaatt attcattatg attaatattg aattttgaat aaatttttat agtatgttac
    10741 ttttaaatgg gatggcttct gtgtacttct aaatagattc atcttaataa gtccattcaa
    10801 tttaaatgtt tggctgtgtt agtaactgac gaatcgcaca agtgcacact catattcaca
    10861 tttactacaa acgccttttt gctcaaacaa aaatatttgt ttacggtagc ttttgaacta
    10921 ccacctgcac acacgaacac tcaaaagatg ctaattttga ttcgacagaa ttttttacaa
    10981 cgtgtttgtt cgaaacgtct tttcaaggtt gatcatttat tatcttttca ttcaataaat
    11041 atgaggttag caatttttat caaaccgcca tagccacatc tcgattcaag gataaatttg
    11101 ttttctcata tcttatctct catgacagtg cacgatatat atcttttcca aatattcatc
    11161 acttttttcc tgttaaaatc atttctatta ttaatgcttt aatgacaact tatttaattt
    11221 attgcattta atgccctgct tcattttcaa atattaactt taatcgacta gctcatcaca
    11281 ctatttaccc tttccaccta tcactactga attcaaaacg atttgtttac aaatcccctt
    11341 atgccaacgg tcgaatttca tcatgtttac atttaattgc aaataccctc ttgaaatatt
    11401 ttaaaattaa tattattctt agcatactag tcggaattat attcaaaaat ttgaggagtg
    11461 acgttcatga atgcttttct tacactgatt aatattattg tattggtgat tttcatcgta
    11521 atattgcaca tgatggctcg caaacatata tcttttgcga aacgtgtgtt tacagcttta
    11581 gggattggta tagtattcgg tgtgttattg catcttattt atggcacaca ttcgaatgtt
    11641 attacatcaa caagtgactg gttcaacatt gtcggacaag gttacgttgc attgttgcaa
    11701 atgattgtta tgcctttaat tttcatttct attgtagctg cattcactaa aattcaaatc
    11761 ggagaaaagt ttgctaaaat tggtagtctc atttttatct ttttaattgg cactgtcacc
    11821 attgctgcga ttgttggtgt tgtgtatgcc ttggtgtttg gtttagatgc atcaacaatt
    11881 aaccttggta acgctgaaca agcacgtgga agcgaaattg ctaaacaagc gaaagattta
    11941 acagcacata cgttaccaca acaaattttg gaattattgc ctaaaaatcc attcttagat
    12001 ttcactggac aacgtgcaac ttccacgatt gctgtcgtga tatttgcttc gtttatagga
    12061 tttgcttatt tacgcgtagc acgcaaacaa cctgatcatg gtgaattgct taagcgtgcg
    12121 attgacgcta tctattcact agtaatggcg attgtgacgt tcgtcttacg tttaacgcca
    12181 tacggtgtat tagcaattat ggccaacaca ttatcgacaa gtgactttgg cgccatttgg
    12241 actttaggaa aattcttaat cgcatcgtac gctgcattaa ttacgatgta tatcattcac
    12301 ttaattattt taagcctgtt aggtattagc ccaattcgct atgttaaaaa gactttggaa
    12361 gtattaatct ttgcattcac atcacgctca agcgcgggtg ccttaccatt gaatgtacaa
    12421 acacaaacaa gacgtctagg cgtgccagaa ggtattgcca atttcgctgc gacatttgga
    12481 ttgtcgattg gacaaaatgg ttgtgcaggc atttaccctg ccatgcttgc aattatggtc
    12541 gcacccgtcg ctaatgtaga aattgattta caatttattg taacgttaat cgctgtcgtg
    12601 attattagtt cattcggcgt tgctggcgtc ggtggcgggg ctacatttgc atccatattg
    12661 gtgttgtcaa cgttaaactt gccagtcgct ttagccggcg tgctcatctc cgttgaacca
    12721 ttgattgaca tgggtagaac agcattaaac gtgaacgatt caatgctagc tggtacaggt
    12781 accgccaagt taactaaaca ttgggataaa gatactttcg attcaaatga taatgctgca
    12841 ttaacttcac actaattcat tgcaagaaat acccgccccc tctagctata cttatcattt
    12901 taagctagag gggggcgggt tattttggta tcttgctatc gctgtattaa gcctgatttt
    12961 tgcaattgtt ctaacatatc taatcttgct ttgtttccta ggaacatctc aatttgctgc
    13021 gaccatgttt ctttgcgatt cccgtttgta cgttgatcat agtactcgct gattgtctgg
    13081 tcgtaatctg ccatttgtgc atactgtgtt tccttatcag catgatactt attatgatgg
    13141 aagacatggt caaatggtaa gcgtggcttg gctgcaccat tttcgtcatc tgcgggttcc
    13201 cctactgcca taccaaatac cgggaagaca tagtcaggta agtctaaaat ttctcgaacg
    13261 cgttcaacat catttcttaa tgatcctaaa aagacaatgc cataccccat atcttcagca
    13321 gttaccgcaa tattttcggc aactaatgct gcatcgattg cacctactag caaaccttcc
    13381 gttgaaccat atgcattttc catatcagtt tcagcatgtt gatcaactaa atgatgacga
    13441 taataatcaa taacaaagac gaataaatag ccattttcta caacataagg ttgtccagaa
    13501 acttctcgta aattttcttt aatcttctca tcgtcgatac caataattga gtatgcttgc
    13561 aggaaacttg acgtcgaagc gctttgtcca gcttctacca atttcttaac aacgtcttca
    13621 cttaaaggtt tattcttaaa ttttctaaca gaatgatgct ttttcacaag attatataca
    13681 tgttctgaca cgttacccac tcctcgatac tttacaatta atttaaaatc ttcatattgc
    13741 aattgtacac gtccaaactt ttttatagca attatttgct tataagttaa taattgaact
    13801 ttttaaaata ttgtctcata ctttaaaaat ccagtttctc actcattacc ttcatccatc
    13861 tcgaacaaat attctcacac cattatcaat aaggttgact tagttgagaa tacaaatctt
    13921 ctttttttac attttttaca ttaaaatcaa gatattaaat atagacacaa ccttttaaat
    13981 catcaactac aaaatccatt atcaaagcac tttatattat ctttctttat tttaaaagta
    14041 atcattcact ttacaaagtt atccagttga ctattctgga tcaaaatgta ataatgctaa
    14101 ctaagagatt agaattatta taattaagcg caatcaattg atagttctaa ttcatactta
    14161 aattcttagg aggaagatat ttatgtcatt aattaacaaa gaaatcttac catttacagc
    14221 gcaagctttc gatccaaaaa aagatcaatt taaagaagtt acacaagaag atttaaaagg
    14281 ttcttggagc gtagtatgct tctatcctgc tgacttctca ttcgtttgtc caactgaatt
    14341 agaagactta caaaaccaat atgaagaatt acaaaaatta ggcgtaaatg tattctcagt
    14401 atcaactgat actcacttcg tacacaaagc atggcatgac cattcagatg caattagcaa
    14461 aatcacttac actatgattg gtgacccatc acaaacaatc actcgtaatt ttgatgtatt
    14521 agatgaagct actggtttag ctcaacgtgg tacattcatt atcgacccag acggtgttgt
    14581 acaagcatct gaaattaacg ctgacggaat tggccgtgac gctagtacat tagctcacaa
    14641 aatcaaagca gctcaatatg ttcgtaaaaa ccctggcgaa gtatgcccag ctaaatggga
    14701 agaaggcgct aaaacattgc aacctggttt agatttagta ggtaaaatct aaggaggcat
    14761 tacaaccatg cttaatgctg atttaaaaca acaacttaaa caactattag aactaatgga
    14821 gggcaacgtt gaattcgttg ccagccttgg ttcagatgat aaatccaaag aacttaaaga
    14881 tttgttgaca gaaattactg atatgtcacc tagactatct ctttctgaaa aatctttaaa
    14941 acgtacacca agtttctcag tcaatcgtcc tggcgaagaa acaggtgtaa catttgcagg
    15001 tattccatta ggtcacgagt ttaactcact tgttttagca attttacagg ttagtggtcg
    15061 tgcacctaaa gaaaaacagt caatcattga ccaaattaaa aaattagaag gttcattcca
    15121 ttttgaaaca ttcattagtt taacgtgtca aaaatgtcct gatgtcgttc aagcacttaa
    15181 cttaatgagt gtgatcaacc ctaacatcac gcattctatg attgatggtg cagtgttccg
    15241 tgaagaatct gaaaacatca tggcagtccc tgctgtcttt ttaaatggcg aagaatttgg
    15301 caatggtcgt atgacaatcc aagatattct ttcgaaacta ggcagtacgg cagatgcatc
    15361 tgagtttgaa aataaagaac cttatgatgt cttaatcgtt ggtggtggtc ctgctagtgg
    15421 tagtgcagcg atttacacag cacgtaaagg tttacgtact ggtatagttg ctgatcgtat
    15481 cggtggccaa gttaatgata ctgctggtat tgagaacttc attactgtta aagaaacaac
    15541 tggttctgaa ttttcttcta acttagcagc gcacattgat caatatgaca ttgatgcaat
    15601 gacaggtata cgtgctacag atatcgaaaa gactgacgaa gcaattaaag ttacgttaga
    15661 aaacggtgct gtcttagaaa gtaaaacagt cattattgct actggtgcag gttggcgtaa
    15721 gctaaacatt ccaggtgaag agcaattgat taataaaggt gttgcattct gccctcactg
    15781 tgacggacct ctatttgaaa ataaagacgt agcagttatc ggtggcggta actctggggt
    15841 tgaagcagca attgaccttg ctggtatcgt taatcatgtt acattattcg aattcgctag
    15901 cgaattaaaa gcagacaacg tgttacaaga tcgtttacgt tctttatcaa atgttgatat
    15961 caaaacaaat gccaaaacta ctgaagttgt cggagaagac catgttacag gtatacgtta
    16021 cgaagacatg aacaccggcg aagaacatct acttaactta gatggtatct ttgttcaaat
    16081 tggtttactt ccaaacacat catggttaaa cgatgctgtt gaattaaacg aacgtggtga
    16141 aattgtgatt gatcgtaaca ataatacgaa tgttcctgga atatttgctg ctggcgatgt
    16201 cacagatcag aagaacaaac aaattatcat ttcaatgggc gctggtgcaa atgcagcatt
    16261 aaatgccttt gactatatta tcagaaacta aagtttcatt catttcactg acagtatctt
    16321 acatttgtta agatgctgtt atttttttat cccgacataa aatcatgtta tgataaactt
    16381 aatatataca acgagatgtg cgataatgct taaagtatgt acctctcgca aacaaatcaa
    16441 tatgaaaaat gatattgaga aagaaaagta tgtgaacgaa attattatgc ttttacgtac
    16501 gatacctacg tctaaattag tacaaccaat tatgcagtta tttatagata aagctatcct
    16561 taacaacgtc caaaatgatg cattattgat agaaaaagca actcatgcac gtaatcattt
    16621 acgtctatgg tcattgcaag gtcatcgtga cattccaaac taattcaatg ttactctatt
    16681 aaacatgaat acatattttt tcaattaaag ataaactaag gagttatatt acattatgaa
    16741 aaagagatta ctactaagta catttttagc atcgacatta attcttacag gttgcgcgtc
    16801 cgatcaatct gataacgaag atcatcatac aagtactggc atccatgcac ctaaaagcgc
    16861 taaaaaatta gaaacaaaag atatctttaa ctcagataag aaaaatagtg atattagtga
    16921 tgcagagatg aagcaagcta tagaaaagta tttgtctgta aatagcgata tacttgataa
    16981 caaatatatt atgcaacata aactagataa acaaattgac agtcaaacaa aagtgactga
    17041 aaaacaagca gaaacactta gccacttatc taacttagct gttaaaaacg atttgcattt
    17101 caagaaattt gtcactgaaa acaatattcc taaagaatat aagaaacccg ttgaattaat
    17161 gatgaattac ttcaaagctt taaatagtac aatcgccaat gttgatgaag atattgaaaa
    17221 gcttagctat caaccgcaaa ataaaatcaa tgttgtcgac gtgccaacaa aatatgccgg
    17281 tgatgttaat aaaaagcaac aagataaaat taaagatttc ttaaaatcta aaggtatcaa
    17341 gagtgatgtt atcgataaat aactgaaagc catcattgcg aaatgtaaca catcattaca
    17401 tgagttttaa ttcatgacaa ttctttatct ttaaaagtga ctaatcaatt tagctaaagt
    17461 catcatttgg gcatcaagca gcatattact gtttgatgcc tttttaattg ccttaaattc
    17521 tctatctatg ccaaaattcc cattagttga cgcatttgac tatataccta acatattcca
    17581 tacttatatt gactctataa acaaaggatg atcatgatga aagttcgaat tacttttatt
    17641 attttagcaa ttttatcgac gattgtttgc ttgttcttag cagcaatgca tccaacagga
    17701 ccaaacacag taacttttga acagccatat ttattcaccc tcaacatcat tataatggta
    17761 ttagtcgcat taccttcact tgttttagct atatatgatt atatgagttt tagaatttct
    17821 tctgctattt tacaatttct aggggctatc tcttggttct ttttatcatt gatattatcg
    17881 ctcacacaat ttacaccttt tacattagcg tcatttataa cttcaattat tttgttcaca
    17941 agcacaatta tcacattagc cattggtggt aagtctgttg aaaagaatga ttccccttaa
    18001 attccaaatg aaaaaaaggt tctgaaggcc gctataaaac acagtttttc agaacctcta
    18061 tacttctatt caatgatata tggtttgcaa ttttctacct ttaaatccac agcttctgcc
    18121 cttgaaactt tgttaaaata aaccatcaaa caacgaatga caacttgatg tgcaacaatg
    18181 acaatatcat ctttttgtgt atcttcattg acaacatgat tcataaaatg ttctacgcgt
    18241 tgatatacat cttcataact ttctccttca ggcgcttttt gtgaaaaact atgacgaaag
    18301 tctttaaagt ttggatcatt gaaatatttt tcatatttcg gattcgcact gatttcatct
    18361 ttatattcac cctcaaatac gccaagtgaa cgttctctta atagaggggt agtcgttgat
    18421 gcaatgtcat atggaaaaat atgttcaaac gtttgctgtg ttcttaataa gtctgaaaca
    18481 tatacatgtt taatctgttt ctctttaaaa taatcacata aatcgtctgc actttttgtg
    18541 ccagtatccg ttaacggcac atctaattgt ccacaaaaat aagatcgaaa atgtttatta
    18601 tcataattcg attttgattc gccatgtcta actaaataaa tcgtcataat attactcctt
    18661 accttatgta tttcatatct accataacac tttgactact aattcgatat caatcttaat
    18721 attctattct aaaa
//
LOCUS       DAXWFK010000037        18625 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.037, whole genome shotgun sequence.
ACCESSION   DAXWFK010000037 DAXWFK010000000
VERSION     DAXWFK010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 18625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18625
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.037"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            393..1730
                     /locus_tag="ACGEXS_001009"
     CDS             393..1730
                     /locus_tag="ACGEXS_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HFF8012029.1"
                     /translation="MKQPILNKLESLNQEEAISLHVPGHKNMTIGHLSQLSMTMDKTE
                     IPGLDDLHHPEEVILESMKQVEKHSDYDAYFLVNGTTSGILSVIQSFSQKKGDILMAR
                     NVHKSVLHALDISQQEGHFIETHQSPLTNHYNKVNLSRLNNDGHKLAVLTYPNYYGET
                     FNVEEVIKSLHQLNIPVLIDEAHGAHFGLQGFPDSTLNYQADYVVQSFHKTLPALTMG
                     SVLYIHKNAPYRETIIEYLSYFQTSSPSYLIMASLESAAEFYKTYDSTVFFDKRAQLI
                     ECLEKKGFEMLQVDDPLKLLIKYEGFTGHDIQNWFMNAHIYLELADDYQALAILPLWH
                     HDDTYLFDSLLRKIEDMILPKKSVSKVKQTQLLTTEGNYKPKRFEYVTWCDLKKAKGK
                     VLARHIVPYPPGIPIIFKGETITENMIELVNEYLETGMIVEGIKNNKILVEDE"
     gene            1732..2349
                     /gene="tmk"
                     /locus_tag="ACGEXS_001010"
     CDS             1732..2349
                     /gene="tmk"
                     /locus_tag="ACGEXS_001010"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFF8012030.1"
                     /translation="MSAFITFEGPEGSGKTTVINEVYHRLVKDYDVIMTREPGGVPTG
                     EEIRKIVLEGNDMDIRTEAMLFAASRREHLVLKVIPALKEGKVVLCDRYIDSSLAYQG
                     YARGIGVEEVRALNEFAINGLYPDLTIYLNVSAEVGRERIIKNSRDQNRLDQEDLKFH
                     EKVIEGYQEIIHNESQRFKSVNADQPLENVVEDTYQTIIKYLEKI"
     gene            2377..2706
                     /locus_tag="ACGEXS_001011"
     CDS             2377..2706
                     /locus_tag="ACGEXS_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFF8012031.1"
                     /translation="MKMIIAIVQDQDSQELADQLVKNNFRATKLATTGGFLRAGNTTF
                     LCGVNDDRVDEILSVINQTCGNREQLVSPITPMGGSADSYIPYPVEVEVGGATVFVMP
                     VDAFHQF"
     gene            2920..3846
                     /locus_tag="ACGEXS_001012"
     CDS             2920..3846
                     /locus_tag="ACGEXS_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta' C-terminal
                     domain-containing protein"
                     /protein_id="HFF8012032.1"
                     /translation="MDEQQQLTNAYHSNKLSHAYLFEGDDAQTMKQVAINFAKLILCQ
                     TDSQCETKVSTYNHPDFMYISTTENAIKKEQVEQLVRHMNQLPIESTNKVYIIEDFEK
                     LTVQGENSILKFLEEPPDNTIAILLSTKPEQILDTIHSRCQHVYFKPIDKEKFINRLV
                     EQNMSKPVAEMISTYTTQIDNAMALNEEFDLLALRKSVIRWCELLLTNKPMALIGIID
                     LLKQAKNKKLQSLTIAAVNGFFEDIIHTKVNVEDKQIYSDLKNDIDQYAQKLSFNQLI
                     LMFDQLTEAHKKLNQNVNPTLVFEQIVIKGVS"
     gene            3847..4650
                     /locus_tag="ACGEXS_001013"
     CDS             3847..4650
                     /locus_tag="ACGEXS_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFF8012033.1"
                     /translation="MPNVIGVQFQKAGKLEYYTPNDIQVDIEDWVVVESKRGIEIGIV
                     KNPLMDIAEEDVVLPLKNIIRIADDKDIDKFNCNERDAENALILCKDIVREQGLDMRL
                     VNCEYTLDKSKVIFNFTADDRIDFRKLVKILAQHLKTRIELRQIGVRDEAKLLGGIGP
                     CGRSLCCSTFLGDFEPVSIKMAKDQNLSLNPTKISGACGRLMCCLKYENDYYEEVRAQ
                     LPDIGEAIETPDGNGKVVALNILDISMQVKLEGHEQPLEYKLEEIETMH"
     gene            4667..5014
                     /gene="yabA"
                     /locus_tag="ACGEXS_001014"
     CDS             4667..5014
                     /gene="yabA"
                     /locus_tag="ACGEXS_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFF8012034.1"
                     /translation="MDRNEIFEKIMRLEMNVNQLSKETSELKALAVELVEENVALQLE
                     NDNLKKVLGNDEPTTIDTANSKPAKAVKKPLPSKDNLAILYGEGFHICKGELFGKHRH
                     GEDCLFCLEVLSD"
     gene            5288..6013
                     /locus_tag="ACGEXS_001015"
     CDS             5288..6013
                     /locus_tag="ACGEXS_001015"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFF8012035.1"
                     /translation="MLKENERFDQLIKEDFSIIQNDDVFSFSTDALLLGHFTKPRTKD
                     IVLDLCSGNGVIPLLLFAKHPRHIEGVEIQKTLVDMARRTFQFNDVDEYLTMHHMDLK
                     NVTKVFKPSQYTLVTCNPPYFKENQQHQHQKEAHKIARHEIMCTLEDCMIAARHLLKE
                     GGRLNMVHRAERLMDVLFEMRKVNIEPKKVVFIYSKVGKSAQTIVVEGRKGGNQGLEI
                     MPPFYIYNEDGNYSEEMKEVYYG"
     gene            6006..6254
                     /locus_tag="ACGEXS_001016"
     CDS             6006..6254
                     /locus_tag="ACGEXS_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFF8012036.1"
                     /translation="MDSHFVYIVKCSDGSLYTGYAKDVNARVEKHNRGQGAKYTKVRR
                     PVHLVYQEMYETKSEALKREYEIKTYTRQKKLRLIKER"
     gene            6256..7095
                     /gene="rsmI"
                     /locus_tag="ACGEXS_001017"
     CDS             6256..7095
                     /gene="rsmI"
                     /locus_tag="ACGEXS_001017"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFF8012037.1"
                     /translation="MAVLYLVGTPIGNLADITYRAVDVLKRVDMIACEDTRVTSKLCN
                     HYDIPTPLKSYHEHNKDKQTAFIIEQLELGLDVALVSDAGLPLISDPGYELVVAAREA
                     NIKVETVPGPNAGLTALMASGLPSYVYTFLGFLPRKEKEKSAVLEQRMHENSTLIIYE
                     SPHRVTDTLKTIAKIDATRQVSLGRELTKKFEQIVTDDVTQLQALIQQGDVPLKGEFV
                     ILIEGAKANNEISWFDDLSINEHVDHYIQTSQMKPKQAIKKVAEERQLKTNEVYNIYH
                     QIS"
     gene            7380..9353
                     /gene="metG"
                     /locus_tag="ACGEXS_001018"
     CDS             7380..9353
                     /gene="metG"
                     /locus_tag="ACGEXS_001018"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFF8012038.1"
                     /translation="MAKETFYITTPIYYPSGNLHIGHAYSTVAGDVIARYKRMQGYDV
                     RYLTGTDEHGQKIQEKAQKAGKTEIEYLDEMIAGIKQLWAKLEILNDDFIRTTEERHK
                     HVVEQVFERLLKQGDIYLGEYEGWYSVPDETYYTESQLVDPQYENGKIIGGKSPDSGH
                     EVELVKEESYFFNISKYTDRLLEFYDQNPDFIQPPSRKNEMINNFIKPGLADLAVSRT
                     SFNWGVHVPSNPKHVVYVWIDALVNYISALGYLSDDESLFNKYWPADIHLMAKEIVRF
                     HSIIWPILLMALDLPLPKKVFAHGWILMKDGKMSKSKGNVVDPNILIDRYGLDATRYY
                     LMRELPFGSDGVFTPEAFVERTNFDLANDLGNLVNRTISMVNKYFDGELPAYQGPLHE
                     LDEEMEAMALETVKSYTESMESLQFSVALSTVWKFISRTNKYIDETTPWVLAKDDSQK
                     DMLGNVMAHLVENIRYAAVLLRPFLTHAPKEIFEQLNINNPQFMEFSSLEQYGVLNES
                     IMVTGQPKPIFPRLDSEAEIAYIKESMQPPATKEEKEEIPSKPQIDIKDFDKVEIKAA
                     TIIDAEHVKKSDKLLKIQVDLDSEQRQIVSGIAKFYTPDDIIGKKVAVVTNLKPAKLM
                     GQKSEGMILSAEKDGVLTLVSLPSAIPNGAVIK"
     gene            9384..10157
                     /locus_tag="ACGEXS_001019"
     CDS             9384..10157
                     /locus_tag="ACGEXS_001019"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFF8012039.1"
                     /translation="MLIDTHVHLNDEQYDDDLSEVITRAREAGVDRMFVVGFNKSTIE
                     RAMKLIDEYDFLYGIIGWHPVDAIDFTEEHLEWIESLAQHPKVIGIGEMGLDYHWDKS
                     PADVQKEVFRKQIALAKRLKLPIIIHNREATQDCIDILLEEHAEEVGGIMHSFSGSPE
                     IADIVTNKLNFYISLGGPVTFKNAKQPKEVAKHVSMERLLVETDAPYLSPHPYRGKRN
                     EPARVTLVAEQIAELKGLSYEEVCEQTTKNAEKLFNLNS"
     gene            10324..10860
                     /gene="rnmV"
                     /locus_tag="ACGEXS_001020"
     CDS             10324..10860
                     /gene="rnmV"
                     /locus_tag="ACGEXS_001020"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFF8012040.1"
                     /translation="MKINEFIVVEGRDDTERVKRAVECDTIETNGSAINEQTLEVIRN
                     AQQSRGVIVLTDPDFPGDKIRSTITEHVKGVKHAYIDREKAKNKKGKIGVEHADLIDI
                     KEALMHVSSPFDEAYESIDKSVLIELGLIVGKDARRRREILSRKLRIGHSNGKQLLKK
                     LNAFGYTEADVRQALEDE"
     gene            10871..11764
                     /gene="rsmA"
                     /locus_tag="ACGEXS_001021"
     CDS             10871..11764
                     /gene="rsmA"
                     /locus_tag="ACGEXS_001021"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFF8012041.1"
                     /translation="MLDNKDIATPSRTRALLDKYGFNFKKSLGQNFLIDVNIINNIID
                     ASDIDAQTGVIEIGPGMGSLTEQLARHAKRVLAFEIDQRLIPVLNDTLSPYDNVTVIN
                     EDILKANIKEAVENHLQDCEKIMVVANLPYYITTPILLNLMQQDIPIDGYVVMMQKEV
                     GERLNAEVGSKAYGSLSIVVQYYTETSKVLTVPKSVFMPPPNVDSIVVKLMQRTEPLV
                     TVDNEEAFFKLAKAAFAQRRKTINNNYQNYFKDGKQHKEVILQWLEQAGIDPRRRGET
                     LSIQDFAKLYEEKKKFPQLEN"
     gene            11864..12127
                     /gene="veg"
                     /locus_tag="ACGEXS_001022"
     CDS             11864..12127
                     /gene="veg"
                     /locus_tag="ACGEXS_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation stimulator Veg"
                     /protein_id="HFF8012042.1"
                     /translation="MPKSILDIKNSIDCHVGNRIVLKANGGRKKTIKRSGILKETYPS
                     VFIVELDQDKHNFERVSYTYTDVLTENVQVSFEEDNHHESIAH"
     gene            12437..13285
                     /gene="ispE"
                     /locus_tag="ACGEXS_001023"
     CDS             12437..13285
                     /gene="ispE"
                     /locus_tag="ACGEXS_001023"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFF8012043.1"
                     /translation="MIYETAPAKINFTLDTLFKRNDGYHEIEMIMTTVDLNDRLTFHK
                     RKDRKIVVEIEHNYVPSNHKNLAYRAAQLFIEQYQLKQGVTISIDKEIPVSAGLAGGS
                     ADAAATLRGLNRLFDIGASLEELALLGSKIGTDIPFCIYNKTALCTGRGEKIEFLNKP
                     PSAWVILAKPNLGISSPDIFKLINLDKRYDVHTKMCYEALENRDYQQLCQSLSNRLEP
                     ISVSKHPQIDKLKNNMLKSGADGALMSGSGPTVYGLARKESQAKNIYNAVNGCCNEVY
                     LVRLLG"
     gene            13299..14123
                     /gene="purR"
                     /locus_tag="ACGEXS_001024"
     CDS             13299..14123
                     /gene="purR"
                     /locus_tag="ACGEXS_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFF8012044.1"
                     /translation="MRYKRSERIVFMTQYLMNHPNKLIPLTFFVKKFKQAKSSISEDV
                     QIIKNTFQKEKLGTVITTAGASGGVTYKPMMSKEEATEVVNEVITLLEEKERLLPGGY
                     LFLSDLVGNPSLLNKVGKLIASIYMEEKLDAVVTIATKGISLANAVANILNLPVVVIR
                     KDNKVTEGSTVSINYVSGSSRKIETMVLSKRTLAENSNVLVVDDFMRAGGSINGVMNL
                     MNEFKAHVKGVSVLVESKEVKQRLIEDYTSLVKLSDVDEYNQEFNVEPGNSLSKFS"
     gene            14140..14520
                     /locus_tag="ACGEXS_001025"
     CDS             14140..14520
                     /locus_tag="ACGEXS_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFF8012045.1"
                     /translation="MKIINTTRLPEALGPYSHATVVNGMVYTSGQIPLNIDGHIVSAD
                     VQAQTKQVLENLKVVLEEAGSDLNSVAKATIFIKDMNDFQKINEVYGQYFNEHKPARS
                     CVEVARLPKDVKVEIELVSKIKEL"
     gene            14590..14892
                     /gene="spoVG"
                     /locus_tag="ACGEXS_001026"
     CDS             14590..14892
                     /gene="spoVG"
                     /locus_tag="ACGEXS_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HFF8012046.1"
                     /translation="MKVTDVRLRKIQTDGRMKALVSITLDEAFVIHDLRVIEGNSGLF
                     VAMPSKRTPDGEFRDIAHPINSDMRQEIQDAVMKVYDETDEVVPDKNATSEDSEEA"
     gene            15082..15213
                     /locus_tag="ACGEXS_001027"
     CDS             15082..15213
                     /locus_tag="ACGEXS_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012047.1"
                     /translation="MRLNDARIFEVRKKLFLKLQRIKNNAFYMLKEYCRLNYNNDEV"
     gene            15236..16588
                     /gene="glmU"
                     /locus_tag="ACGEXS_001028"
     CDS             15236..16588
                     /gene="glmU"
                     /locus_tag="ACGEXS_001028"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFF8012048.1"
                     /translation="MRRHAIILAAGKGTRMKSKKYKVLHEVAGKPMVEHVLESVKGSG
                     VDQVVTIVGHGAESVKGHLGERSLYSFQEEQLGTAHAVQMAKSHLEDKEGTTIVVCGD
                     TPLITKETLETLIAHHEDANAQATVLSASIQQPYGYGRIVRNASGRLERIVEEKDATQ
                     AEKDINEISSGIFAFNNKTLFEKLTQVKNDNAQGEYYLPDVLSLILNDGGIVEVYRTN
                     DVEEIMGVNDRVMLSQAEKAMQRRTNHYHMLNGVTIIDPDSTYIGPDVTIGSDTVIEP
                     GVRINGRTEIGEDVVIGQYSEINNSTIENGACIQQSVVNDASVGANTKVGPFAQLRPG
                     AQLGADVKVGNFVEIKKADLKDGAKVSHLSYIGDAVIGERTNIGCGTITVNYDGENKF
                     KTIVGKDSFVGCNVNLVAPVTIGDDVLVAAGSTITDDVPNDSLAVARARQTTKEGYRK
                     "
     gene            16735..17700
                     /locus_tag="ACGEXS_001029"
     CDS             16735..17700
                     /locus_tag="ACGEXS_001029"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFF8012049.1"
                     /translation="MLNNEYKNSSLKIFSLKGNEALAQEVADQVGIELGKCSVKRFSD
                     GEIQINIEESIRGCDVFIIQPTSYPVNLHLMELLIMIDACKRASAATINIVVPYYGYA
                     RQDRKARSREPITAKLVANLIETAGATRMIALDLHAPQIQGFFDIPIDHLMGVPILAK
                     HFKDDPNINPEECVVVSPDHGGVTRARKLADILKTPIAIIDKRRPRPNVAEVMNIVGE
                     IEGRTAIIIDDIIDTAGTITLAAQALKDKGAKEVYACCTHPVLSGPAKERIENSAIKE
                     LIVTNSIHLDEDRKPSNTKELSVAGLIAQAIIRVYERESVSVLFD"
     gene            17850..18503
                     /locus_tag="ACGEXS_001030"
     CDS             17850..18503
                     /locus_tag="ACGEXS_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFF8012050.1"
                     /translation="MASLKSIIRQGKQTRSDLKQLRKSGKVPAVVYGYGTKNVSVKVD
                     EVEFIKVIREVGRNGVIELGVGSKTIKVMVADYQFDPLKNQITHIDFLAINMSEERTV
                     EVPVQLVGEAVGAKEGGVVEQPLFNLEVTATPDNIPEAIEVDITELNINDSLTVADVK
                     VTGDFKIENDSAESVVTVVAPTEEPTEEEIEAMEGEQQTEEPEVVGESKEDEEKTEE"
BASE COUNT         6756 a         2511 c         3720 g         5638 t
ORIGIN      
        1 ggatggtagc caacttacgt tccgctagag tagaacgttg ctagacaaga aatgaatgcg
       61 atgagccgca ttgagtgtga aattgatatt ttaataatgt gcacttttga ttatttaaat
      121 ctgtgactag aagtataagt gaagacattc agaagtatta taaaaagtga acagcagtaa
      181 gatagttttt aatcataaat catcttactg ctgtttttag attttatgtc taatatcttt
      241 taaatcgaag tacaaaaagg aaattaatta ttatacaata gacaagctat tgcataagta
      301 acactaactt ttgtcaaaga agtgttacta tataattaat acttttgaaa gtaactaatt
      361 ccaaaacagt gtaaataaag gaagcgtata tcatgaagca acctatttta aataaattag
      421 aaagtttaaa tcaagaagaa gcgatttctt tgcatgttcc gggtcataaa aatatgacta
      481 tcggtcattt atctcaatta tcaatgacaa tggataaaac tgaaatacct ggattagatg
      541 atttacatca tcctgaagaa gtcattttgg aaagtatgaa gcaggtggag aaacattcag
      601 attatgatgc ttatttctta gtgaatggca ccacttcagg aatattatct gtcatccagt
      661 ctttttcaca gaaaaaaggc gatatcttaa tggcaagaaa tgtacataaa tctgtgttac
      721 atgcgctcga tattagccaa caagaagggc attttattga aacgcatcaa agtccgttaa
      781 cgaatcatta taataaagtt aatttaagcc gtttgaataa tgacggtcac aaacttgctg
      841 tgttgactta tcctaactat tacggtgaaa catttaatgt agaagaggtt atcaaatctt
      901 tgcaccaatt aaatattcct gtactcattg acgaagcaca cggcgcgcac tttggattgc
      961 aaggatttcc agattctaca ttaaattatc aagctgacta tgttgttcaa tcttttcata
     1021 aaacgttacc agctttaacg atgggctcgg tactttatat tcataaaaat gcaccttata
     1081 gagaaactat tatagaatat ctaagctact tccaaacatc tagtccttcg tatttgatta
     1141 tggctagttt agagtcagct gccgagttct ataaaacata tgatagtacc gtgttttttg
     1201 ataagagagc gcaattaatc gaatgtttgg agaagaaggg ttttgaaatg cttcaagttg
     1261 atgatccgtt gaagttgctg ataaaatatg aaggttttac aggtcatgat attcaaaatt
     1321 ggtttatgaa tgcacatatc tatttagaat tagcggacga ctatcaagca ttagcgatat
     1381 tgccgttatg gcatcatgat gatacgtatt tatttgattc gcttttacgt aaaattgaag
     1441 atatgatttt accgaaaaaa tcagtttcta aagttaaaca aacacaactt ttaacaactg
     1501 aaggtaacta taaaccaaaa cgctttgaat atgttacttg gtgtgatttg aaaaaggcaa
     1561 aaggtaaagt tctggcgcga catattgtcc cgtatccgcc agggattcct attattttca
     1621 aaggagaaac aataactgaa aatatgatag aattggtaaa tgaatatctg gaaactggaa
     1681 tgatagttga aggaattaaa aataataaaa ttttagttga ggatgaataa aatgtcagct
     1741 tttataactt ttgagggccc agaaggctct ggaaaaacaa ctgtaattaa tgaagtttac
     1801 catagattag taaaagatta tgatgtcatt atgactagag aaccaggtgg tgttcctact
     1861 ggtgaagaaa tacgtaaaat tgtattagaa ggcaatgata tggacattag aactgaagca
     1921 atgttatttg ctgcatctag aagagaacat cttgtattaa aggtcatacc agctttaaaa
     1981 gaaggtaagg ttgtgttgtg tgatcgctat atcgatagtt cattagctta tcaaggttat
     2041 gctagaggga ttggcgttga agaagtaaga gcattaaacg aatttgcaat aaatggatta
     2101 tatccagact tgacgattta tttaaatgtt agtgctgaag taggtcgcga acgtattatt
     2161 aaaaattcaa gagatcaaaa tagattagat caagaagatt taaagtttca cgaaaaagta
     2221 attgaaggtt accaagaaat cattcataat gaatcacaac ggttcaaaag cgttaatgca
     2281 gatcaacctc ttgaaaatgt tgttgaagac acgtatcaaa ctatcatcaa atatttagaa
     2341 aagatatgat ataattgtta gaagaggtgt tataaaatga aaatgattat agcgatcgta
     2401 caagatcaag atagtcagga acttgcagat caacttgtta aaaataactt tagagcaaca
     2461 aaattggcaa caacaggtgg gtttttaaga gcgggtaata caacattctt atgtggtgtc
     2521 aatgatgacc gtgtagatga aatattgtct gtgattaatc aaacgtgtgg taatagagaa
     2581 cagttggttt cacctattac acctatggga ggcagtgcgg attcgtacat tccatatcca
     2641 gttgaagttg aagttggcgg tgctactgta tttgttatgc cagttgatgc attccatcaa
     2701 ttttaattct ataatacaat catcaattag agatacttaa aatagtgtat taataagtta
     2761 ttaacaattt tgggttgctt gactgcgact agttcagatg ccaatagatt tgatttttgt
     2821 ggttctaaaa ataatcacaa atcatggtcg ctattgttgc agtaattagt tgctctttgg
     2881 caaccttttt atataaaagc aaaagggagt ttgtaatgaa tggatgaaca gcaacaattg
     2941 acgaatgcat atcattcaaa taaattatcg catgcctatt tatttgaagg tgatgatgca
     3001 caaacgatga aacaagttgc gattaatttt gcaaagctta ttttatgtca aacagatagt
     3061 caatgtgaaa caaaggttag tacatataat catccagact ttatgtatat atcaacaact
     3121 gagaatgcaa ttaagaaaga acaagttgaa caacttgtgc gtcatatgaa tcaacttcct
     3181 atagaaagca caaataaagt gtacatcatt gaagactttg aaaagttaac tgttcaaggg
     3241 gaaaacagta tcttgaaatt tcttgaagaa ccaccggaca atacgattgc tattttattg
     3301 tctacaaaac ctgagcaaat tttagacaca atccattcaa ggtgtcagca tgtatatttc
     3361 aagcctattg ataaagaaaa gtttataaat agattagttg aacaaaacat gtctaagcca
     3421 gtagctgaaa tgattagtac ttatactacg caaatagata atgcaatggc tttaaatgaa
     3481 gaatttgatt tattagcatt aaggaaatca gttatacgtt ggtgtgaatt gttgcttact
     3541 aataagccaa tggcacttat aggtattatt gatttattga aacaggctaa aaataaaaaa
     3601 ctgcaatctt taactattgc agctgtgaat ggtttcttcg aagatatcat acatacaaag
     3661 gtaaatgtag aggataaaca aatatatagt gatttaaaaa atgatattga tcaatatgcg
     3721 caaaagttgt cgtttaatca attaattttg atgtttgatc aactgacgga agcacataag
     3781 aaattgaatc aaaatgtaaa tccaacgctt gtatttgaac aaatcgtaat taagggtgtg
     3841 agttagatgc caaatgtaat aggtgttcag tttcaaaaag cgggaaaatt agaatattat
     3901 acacctaatg atatacaagt agatatagaa gactgggtag ttgtcgaatc taaaagaggc
     3961 atagagatag gtattgttaa aaatccatta atggatattg ctgaagagga tgttgtgtta
     4021 cctcttaaaa atattattcg cattgctgat gacaaagata ttgataaatt taattgtaat
     4081 gaacgagatg ctgaaaatgc attaatacta tgtaaagaca ttgtaagaga acaaggtttg
     4141 gacatgcgtt tagtcaattg cgaatataca ttagataaat cgaaagttat ttttaatttt
     4201 acggcggatg atcgtattga ttttagaaaa ttagtaaaaa tattagcgca acatttaaaa
     4261 acacgtatcg agttgagaca aattggtgta agggatgaag ccaaattgct tggcggtatc
     4321 ggaccttgtg gtaggtcgtt atgttgttct acatttttag gggattttga accagtatcg
     4381 attaagatgg ctaaggatca aaatttatca ttaaatccaa ctaaaatttc tggtgcatgt
     4441 ggtcgtttga tgtgttgttt aaaatatgaa aatgactatt atgaggaagt acgtgcacaa
     4501 ttacctgata ttggtgaagc aattgaaacg cctgatggta acgggaaagt agttgcttta
     4561 aatatattag acatttctat gcaggtgaag cttgagggac atgaacagcc acttgaatat
     4621 aaattagaag aaatagaaac tatgcattaa ggaggcatta ttacatttgg atcgcaatga
     4681 aatatttgaa aaaataatgc gtttagaaat gaatgtcaat caactttcaa aggaaacttc
     4741 agaattaaag gcacttgcag ttgaattagt agaagaaaat gtagcgcttc aacttgaaaa
     4801 tgataatttg aaaaaggtgt tgggcaatga tgaaccaact actattgata ctgcgaattc
     4861 aaaaccagca aaagctgtga aaaagccatt accaagtaaa gataatttgg ctatattgta
     4921 tggagaagga tttcatattt gtaaaggcga attatttgga aaacatcgac atggtgaaga
     4981 ttgtctgttc tgtttagaag ttttaagtga ttaatcaagc acactcaaat agtgttataa
     5041 ttataaatga atatggtttg gataagtctg agacaatgca tgtttcaggc tttaattgtg
     5101 tataaagttt tggtgattgc ataagagatg gcggtactaa atgttattat taagtgtgca
     5161 cgcagtatca ttagttataa aatgtagctg ttaaaagtca aaaatacatc gaatgtagtt
     5221 aggcatataa tataaaaaga gttttcaatt actcaataga aaaaggttgt cttcatagga
     5281 gttaaaaatg ttaaaagaga atgaacgatt tgatcaacta atcaaagaag attttagtat
     5341 tattcaaaat gatgatgttt tttcattttc aacggatgct ttgttgttag ggcattttac
     5401 aaaacctaga acaaaagata ttgtgttgga cttatgttca ggcaatgggg tgataccctt
     5461 gttattgttt gcgaaacatc cacgacatat agaaggtgtt gagattcaaa aaacacttgt
     5521 cgatatggcg cgacgcacat ttcaattcaa tgatgttgat gaatatttaa caatgcatca
     5581 catggatttg aaaaacgtta ctaaagtatt taaaccttca caatatactt tagtaacgtg
     5641 taatccgcct tattttaaag agaatcagca acaccaacat caaaaagaag cacataagat
     5701 agcgagacat gagattatgt gtacacttga agattgcatg attgcagccc gtcatttatt
     5761 aaaagaaggt ggcaggctaa acatggtaca tcgtgcagag agactaatgg atgtcttgtt
     5821 tgaaatgaga aaagtgaata ttgaacctaa gaaagtcgtt tttatatata gtaaagtagg
     5881 gaaatcagca caaacgatag tagtagaagg tcgaaaaggt ggaaatcaag gtttagaaat
     5941 catgccccca ttttatattt ataatgaaga tggtaattat agcgaagaaa tgaaggaagt
     6001 atattatgga tagtcatttt gtatatattg taaaatgtag tgatggaagt ttatatacag
     6061 gatacgctaa agacgttaat gcacgtgttg aaaaacataa ccgaggtcaa ggagccaaat
     6121 atacgaaagt aagacgtccg gtgcatttag tttatcaaga aatgtatgag acaaagtctg
     6181 aagcattgaa gcgtgaatat gaaattaaaa cttataccag acaaaagaaa ttgcgattaa
     6241 ttaaggagcg atagtatggc tgtattatat ttagtgggca caccaattgg taatttagca
     6301 gatattactt atagagcagt tgatgtattg aaacgtgttg atatgattgc ttgtgaagac
     6361 actagagtaa ctagtaaact gtgtaatcat tatgatattc caactccatt aaagtcatat
     6421 cacgaacata acaaggataa gcagactgct tttatcattg aacagttaga attaggtctt
     6481 gacgttgcgc tcgtatctga tgctggattg cccttaatta gtgatcctgg atacgaatta
     6541 gtagtggcag ccagagaagc taatattaaa gtagagactg tgcctggacc taatgctggg
     6601 ctgacggctt tgatggctag tggattacct tcatatgtat atacattttt aggatttttg
     6661 ccacgaaaag agaaagaaaa aagtgctgta ttagagcaac gtatgcatga aaatagcaca
     6721 ttaattatat acgaatcacc gcatcgtgtg acagatacat taaaaacaat tgcaaagata
     6781 gatgcaacac gacaagtatc actagggcgt gaattaacta agaagttcga acaaattgta
     6841 actgatgatg taacacaatt acaagcattg attcagcaag gcgatgtacc attgaaaggc
     6901 gaattcgtta tcttaattga aggtgctaaa gcgaacaatg agatatcgtg gtttgatgat
     6961 ttatctatca atgagcatgt tgatcattat attcaaactt cacagatgaa accaaaacaa
     7021 gctattaaaa aagttgctga agaacgacaa cttaaaacga atgaagtata taatatttat
     7081 catcaaataa gttaatcact ttatcgatta tatgaaattt taaacgattt tataaacgca
     7141 agctgtaatt ttaaatggta agttatcatt ttgcattgat actgataaaa tgatgttgac
     7201 tatgataaaa aaatgatgac atcgacgttt tttaatgtaa aataaataca ttgaaagtaa
     7261 taaatacctt aacattgaat aagatgaaaa tgagatgacg agataaatgt tcgcgtccgt
     7321 tgaaatgcat agaaatctta gatattattt gaagtgagac attacgagga ggaacagtta
     7381 tggctaaaga aacattttat ataacaaccc caatatacta tcctagtggg aatttacata
     7441 taggacatgc atattctaca gtggctggag atgttattgc aagatataag agaatgcaag
     7501 gatatgatgt tcgctatttg actggaacgg atgaacacgg tcaaaaaatt caagaaaaag
     7561 ctcaaaaagc tggtaagaca gaaattgaat atttggatga gatgattgct ggaattaaac
     7621 aattgtgggc taagcttgaa attttaaatg atgattttat cagaacaact gaagaacgtc
     7681 ataaacatgt cgttgagcaa gtgtttgaac gtttattaaa gcaaggtgat atctatttag
     7741 gtgaatatga aggttggtat tctgttccgg atgaaacata ctatacagag tcacaattag
     7801 tagacccaca atacgaaaac ggtaaaatta ttggtggcaa aagtccagat tctggacacg
     7861 aagttgaact agttaaagaa gaaagttatt tctttaatat tagtaaatat acagaccgtt
     7921 tattagagtt ctatgaccaa aatccagatt ttatacaacc accatcaaga aaaaatgaaa
     7981 tgattaacaa cttcattaaa ccaggacttg ctgatttagc tgtttctcgt acatcattta
     8041 actggggtgt ccatgttccg tctaatccaa aacatgttgt ttatgtttgg attgatgcgt
     8101 tagttaacta tatttcagca ttaggctatt tatcagatga tgagtcacta tttaacaaat
     8161 actggccagc agatattcat ttaatggcta aggaaattgt gcgattccac tcaattattt
     8221 ggcctatttt attgatggca ttagacttac cgttacctaa aaaagtcttt gcacatggtt
     8281 ggattttgat gaaagatgga aaaatgagta aatctaaagg taatgtcgta gaccctaata
     8341 ttttaattga tcgctatggt ttagatgcta cacgttatta tctaatgcgt gaattaccat
     8401 ttggttcaga tggcgtattt acacctgaag catttgttga gcgtacaaat ttcgatctag
     8461 caaatgactt aggtaactta gtaaaccgta cgatttctat ggttaataag tactttgatg
     8521 gcgaattacc agcgtatcaa ggtccacttc atgaattaga tgaagaaatg gaagctatgg
     8581 ctttagaaac agtgaaaagc tacactgaaa gcatggaaag tttgcaattt tctgtggcat
     8641 tatctacggt atggaagttt attagtagaa cgaataagta tattgacgaa acaacgcctt
     8701 gggtattagc taaggacgat agccaaaaag atatgttagg caatgtaatg gctcacttag
     8761 ttgaaaatat tcgttatgca gctgtattat tacgtccatt cttaacacat gcgccgaaag
     8821 agatttttga acaattgaac attaacaatc ctcaatttat ggaatttagt agtttagagc
     8881 aatatggtgt gcttaatgag tcaattatgg ttactgggca acctaaacct attttcccaa
     8941 gattggatag cgaagcggaa attgcatata tcaaagaatc aatgcaaccg cctgctacta
     9001 aagaggaaaa agaagagatt cctagcaaac ctcaaattga tattaaagac tttgataaag
     9061 ttgaaattaa ggcagcaacg attattgatg ctgaacatgt taagaagtca gataagcttt
     9121 taaaaattca agtagactta gattctgaac aaagacaaat tgtatcagga attgccaaat
     9181 tctatacacc agatgatatt attggtaaaa aagtagcagt tgttactaac ctgaaaccag
     9241 ctaaattaat gggacaaaaa tctgaaggta tgatattatc tgctgaaaaa gatggtgtat
     9301 taaccttagt aagtttacca agtgcaattc caaatggtgc agtgattaaa taactgtatt
     9361 tttaaaaatt aggagagata attatgttaa tcgatacaca tgtccattta aatgatgagc
     9421 aatacgatga tgatttgagt gaagtgatta cacgtgctag agaagcaggt gttgatcgta
     9481 tgtttgtagt tggttttaac aaatcgacaa ttgaacgcgc gatgaaatta atcgatgagt
     9541 atgatttttt atatggcatt atcggttggc atccagttga cgcaattgat tttacagaag
     9601 aacacttgga atggattgaa tctttagctc agcatccaaa agtgattggt attggtgaaa
     9661 tgggattaga ttatcactgg gataaatctc ctgcagatgt tcaaaaggaa gtttttagaa
     9721 agcaaattgc tttagctaag cgtttgaagt taccaattat cattcataac cgtgaagcaa
     9781 ctcaagactg tatcgatatc ttattggagg agcatgctga agaggtaggc gggattatgc
     9841 atagctttag tggttctcca gaaattgcag atattgtaac taataagctg aatttttata
     9901 tttcattagg tggacctgtg acatttaaaa atgctaaaca gcctaaagaa gttgctaagc
     9961 atgtgtcaat ggagcgtttg ctagttgaaa ccgatgcacc gtatctttcg ccacatccgt
    10021 atagagggaa gcgaaatgaa ccggcgagag taactttagt agctgaacaa attgctgaat
    10081 taaaaggctt atcttatgaa gaagtgtgcg aacaaacaac taaaaatgca gagaaattgt
    10141 ttaatttaaa ttcataaagt taaaagtgag aaagatcacc gccataaatg taaacgatgc
    10201 tatattcgtt taatatgcta tggttctttc tcactttttt aaattaaaat atcgtgcatg
    10261 tggaatacgt gcgatagaga tggttagagc tttgaaatta agaattgtag gaaggcgttt
    10321 taaatgaaaa tcaatgagtt tatagttgta gaaggacgag atgatactga gcgtgttaaa
    10381 cgagctgttg aatgtgatac gattgaaacg aatggtagtg ccatcaacga acaaacttta
    10441 gaagtaatta gaaatgctca acaaagtcga ggcgttattg tattaacaga tccagatttc
    10501 ccaggagata aaattagaag tacaattact gaacatgtca aaggtgttaa acatgcgtat
    10561 attgatagag aaaaagctaa aaataaaaaa gggaaaattg gtgttgaaca tgccgactta
    10621 attgatatta aagaagcgtt aatgcatgtt agttcaccct ttgatgaagc ttatgaatca
    10681 attgataaat ctgtgctaat agagttgggg ttaattgttg ggaaagatgc aaggcgccgt
    10741 agagaaattt taagtagaaa attgcgaatc ggccattcca atggtaagca gttattgaaa
    10801 aagttaaatg catttggtta taccgaagcg gatgtaaggc aagctttaga agatgaatga
    10861 ggaagtgaaa atgttggata ataaagatat tgcaacacca tcaagaacgc gagcgttgtt
    10921 agataaatat ggctttaatt ttaaaaaaag tttaggacag aactttttga tagatgtgaa
    10981 tatcattaat aatatcattg atgcaagtga tattgatgca caaactgggg tgattgaaat
    11041 tggtccaggc atggggtcat tgacagaaca attggccaga catgctaaaa gagtattggc
    11101 atttgaaatt gatcaacgtt taatacctgt attaaatgat acactatcac cttatgataa
    11161 tgtgacggtg attaatgaag atattttaaa agcgaatatt aaagaagctg ttgaaaatca
    11221 tttacaagat tgtgaaaaaa taatggttgt tgcaaacctg ccgtactata ttacgacgcc
    11281 aattttatta aatttgatgc aacaagatat accaattgat ggctacgtgg tgatgatgca
    11341 aaaagaagtg ggcgaacgct taaatgctga agtaggttca aaagcatatg gttcgttatc
    11401 aattgtcgta caatactata cagagactag taaagtatta acggtaccta aatctgtatt
    11461 tatgccacca cctaatgttg attcaatagt tgtaaaactg atgcagagaa ctgaaccgtt
    11521 agtaacagta gataacgagg aagcattctt taagttagca aaagcagcat ttgcacaaag
    11581 aagaaagaca attaacaata actatcaaaa ttattttaaa gatggtaaac aacacaaaga
    11641 agtgatttta caatggttgg aacaagcagg tattgatcca agacgtcgcg gtgaaacgct
    11701 atctattcaa gattttgcta aattgtatga agaaaagaaa aaattccctc aattagaaaa
    11761 ttaaatgatt gacaaagcaa agcactattg ttaaaattta aattttgttt gacgaaaacg
    11821 ttgcaaatat ggtattatgt aacttgtagc gaggtggagc aatatgccaa aatcaatttt
    11881 ggacatcaaa aattctattg attgtcatgt aggaaatcgt attgtactga aagccaatgg
    11941 aggccgtaag aaaacaataa aacgttctgg aattttaaaa gaaacatatc cgtcagtttt
    12001 cattgttgag ttagatcaag acaaacacaa ctttgagaga gtatcttata catacactga
    12061 tgtgttaact gaaaatgttc aagtttcatt tgaagaggat aatcatcacg aatcaattgc
    12121 acactaaata agacatatag agatgttaga cgtttcttag tataagaagt aaatattatg
    12181 ataattattt gagtgttggg cattatgttc aatactcttt ttatttacaa aatgtttaac
    12241 actgatgttt cgcttataga tttttcagta aatggataat tgtatttata aacacaaata
    12301 caagtaaata ctaagtaatt agatggagaa aattactttt ttattaaaaa aacactaaaa
    12361 aacaaattaa aatgtcaaat attaattctc tttatgttaa aatcatcata ttaagataac
    12421 gaaaagaggg cggaaaatga tatatgaaac ggcaccagcc aaaattaatt ttacgctcga
    12481 tacacttttt aaaagaaatg atggctatca tgagattgaa atgataatga caacagttga
    12541 tttaaatgat cgtttaactt ttcataaaag aaaagatcga aagatagttg ttgagattga
    12601 acataattat gtgccttcta atcataaaaa tctcgcatat cgtgcagcgc aactatttat
    12661 tgagcaatat caactaaagc aaggtgtaac aatttctatc gataaagaaa tacctgtttc
    12721 tgctggctta gctggaggtt cggctgatgc agcagcaacg ttaagaggat tgaatcgact
    12781 ttttgatata ggggcgagtt tggaagaatt ggctctacta ggcagtaaaa tcgggacaga
    12841 tattccgttt tgtatttata ataaaactgc actatgtact ggaagaggag agaaaatcga
    12901 gtttttaaat aaaccacctt cagcttgggt gattcttgct aaaccaaact taggcatatc
    12961 atcaccagat atatttaagt tgattaattt agataagcgt tacgacgtac atacgaaaat
    13021 gtgttatgag gccttagaaa atcgagatta tcaacaatta tgtcaaagtt tgtctaatcg
    13081 attagagcca atttctgttt caaaacaccc acaaatcgat aaattaaaaa ataatatgtt
    13141 gaaaagtggt gcagatggtg cgttaatgag tggaagcgga cctactgtgt atgggctagc
    13201 acgaaaagaa agccaagcaa aaaatattta taatgcagtt aacggttgtt gtaatgaagt
    13261 gtacttagtt agactattag gatagaaggg ttgaaaagat gagatataaa cgaagcgaga
    13321 gaattgtttt tatgacgcaa tatttgatga accatccgaa taaattgatt ccattaactt
    13381 tttttgtgaa aaaatttaaa caggcgaagt cttcaataag tgaagatgtc caaattataa
    13441 aaaatacatt ccaaaaagaa aagttaggta cagtaattac tactgctggc gcaagtggtg
    13501 gtgttacgta taaaccaatg atgagtaaag aagaggcgac tgaagttgtt aatgaggtca
    13561 ttactctatt agaagagaaa gaacgtttgt tacctggcgg atatttattt ttatcagatt
    13621 tggtaggtaa tccatcgcta ctaaacaaag ttggtaagtt aattgccagt atttacatgg
    13681 aagaaaaatt agatgctgtt gttaccattg cgacaaaagg tatttcattg gcaaatgcgg
    13741 ttgctaatat tttaaattta ccagtagtag tgattagaaa agacaacaag gtgactgaag
    13801 gttctacagt ttcaattaat tacgtttcag gatcttcaag aaaaatagaa acaatggtac
    13861 tttcgaagag aactttagca gaaaattcaa atgttttagt tgtcgatgat tttatgaggg
    13921 ctggtggctc tattaatggt gttatgaatt taatgaatga gtttaaagcc catgtaaaag
    13981 gggtatcagt acttgtagaa tcaaaagaag ttaaacaaag attgattgaa gattatactt
    14041 ccttagtgaa attatctgat gtagatgaat ataatcaaga gtttaacgta gaacctggca
    14101 acagtttatc taagttttca taaaaggagt tttagtatta tgaaaatcat taacacaaca
    14161 agattaccgg aagcacttgg accatattcg catgcaacag ttgtgaatgg tatggtttat
    14221 acttctggtc agattccatt gaatattgat ggacatatcg taagcgctga tgttcaagca
    14281 cagacaaaac aagttttaga aaatttaaag gttgttttgg aagaagcagg atctgatttg
    14341 aattctgttg cgaaagcgac cattttcatt aaagatatga atgatttcca aaaaataaat
    14401 gaagtgtatg gtcaatattt taatgaacac aagccagcgc gtagttgtgt agaggttgcg
    14461 cgtttgccaa aagatgtgaa agtagaaatt gaattagtaa gtaaaattaa ggaattataa
    14521 ttttcgatta atatgtttaa tcaagcttct aaataaaaca gagatatata ctataggggg
    14581 gctcactaca tgaaagtgac agatgtaaga cttagaaaaa tacaaacaga tggacgaatg
    14641 aaagcactcg tttccattac attagatgaa gctttcgtaa ttcatgattt acgtgtaatt
    14701 gaaggaaact ctggcttgtt cgttgcaatg ccaagtaaac gtacaccaga tggtgaattc
    14761 cgcgacatcg cgcatcctat taattcagat atgagacaag aaattcaaga tgcagtgatg
    14821 aaagtatatg atgaaacaga tgaagtagta ccagataaaa acgctacatc agaagattca
    14881 gaagaagctt aatcaatttt atatttagcg atgtaataca tttgcaataa gttgatttga
    14941 tactgtcgat aaagcataaa gctttgtcgg cagttttttt agtttgtatt aatgtttttt
    15001 tatttttaat gaaaggctaa taaatatata cgttaacaga ttatgatgat atgaaaatta
    15061 ttgattcatt gctgataaaa attgcgtttg aatgatgctc gtatttttga agtaagaaaa
    15121 aagttgtttt taaaattaca acgaattaaa aacaatgcct tttatatgtt gaaagagtat
    15181 tgcagattaa attataataa tgacgaagtg taaaatttaa tgggggttaa tgttcatgcg
    15241 aagacacgcg ataattttgg cagcaggtaa aggcacaaga atgaaatcta aaaagtataa
    15301 agtgctacac gaggttgctg ggaaacctat ggtcgaacat gtattggaaa gtgtgaaagg
    15361 ctctggtgtc gatcaagttg taaccatcgt aggacatggt gctgaaagtg taaaaggaca
    15421 tttaggcgag cgttctttat acagttttca agaggaacaa ctcggtactg cgcatgcagt
    15481 gcaaatggcg aaatcacact tagaagacaa ggaaggtacg acaatcgttg tatgtggtga
    15541 cacaccgctc atcacaaagg aaacattaga aacattgatt gcgcatcacg aggatgctaa
    15601 tgctcaagca actgtattat ctgcatcgat tcaacaacca tatggatacg gaagaatcgt
    15661 tcgaaatgcg tcaggtcgtt tagaacgcat agttgaagag aaagatgcaa cgcaagctga
    15721 aaaggatatt aatgaaatta gttcaggtat ttttgcgttt aataataaaa cgttgtttga
    15781 aaaattaaca caagtgaaaa atgataatgc gcaaggtgaa tattacctcc ctgatgtatt
    15841 gtcgttaatt ttaaatgatg gcggcatcgt agaagtctat cgtaccaatg atgttgaaga
    15901 aatcatgggt gtaaatgatc gtgtaatgct tagtcaggct gagaaggcga tgcaacgtcg
    15961 tacgaatcat tatcacatgc taaatggtgt gacaatcatc gatcctgaca gcacttatat
    16021 tggtccagac gttacaattg gtagtgatac agtcattgaa ccaggcgtac gaattaatgg
    16081 tcgtacagaa attggcgaag atgttgttat tggtcagtac tctgaaatta acaatagtac
    16141 gattgaaaat ggtgcatgta ttcaacagtc tgttgttaat gatgctagcg taggagcgaa
    16201 tactaaggtc ggaccgtttg cgcaattgag accaggcgcg caattaggtg cagatgttaa
    16261 ggttggaaat tttgtagaaa ttaaaaaagc agatcttaaa gatggtgcca aggtttcaca
    16321 tttaagttat attggcgatg ctgtaattgg cgaacgtact aatattggtt gcggaacgat
    16381 tacagttaac tatgatggtg aaaataaatt taaaactatc gtcggcaaag attcatttgt
    16441 aggttgcaat gttaatttag tagcacctgt aacaattggt gatgatgtat tggtggcagc
    16501 tggttccaca atcacagatg acgtaccaaa tgacagttta gctgtggcaa gagcaagaca
    16561 aacaacaaaa gaaggatata ggaaataatc atttacgtat ttaaaatggc taggataaaa
    16621 ggataatcct atgtaatatt aatgtaatct ttatgattta atgattcgca tagtaatgga
    16681 gttacatttt atatataata gtaattgcgt aagtaaataa ttggaggact ataaatgtta
    16741 aataatgaat ataagaattc gtcattaaag attttttcat tgaaaggaaa cgaagcatta
    16801 gcgcaagaag ttgctgacca agtaggaatt gaactaggta aatgttcagt taaacgtttt
    16861 agtgatggag aaattcaaat taatatcgaa gagagtattc gtggttgtga cgtatttatt
    16921 attcaaccaa catcatatcc tgtgaatcta catttaatgg aattattaat tatgattgat
    16981 gcttgtaaac gtgcttctgc agcaacaatc aatattgtag tgccatatta tggatatgca
    17041 agacaagata gaaaagcccg tagccgtgag ccaatcactg ctaaattagt tgcaaactta
    17101 atcgaaacag ctggcgcaac tcgtatgatt gcgttagact tacatgcacc acaaattcaa
    17161 ggattctttg atattccaat tgaccactta atgggtgtgc caattcttgc taaacatttc
    17221 aaagatgatc cgaatattaa cccagaagaa tgtgtcgttg tttcaccaga ccatggcggc
    17281 gttacacgtg cacgtaaatt agctgacatt ttaaaaactc caattgcaat tatagataaa
    17341 cgtcgtccta gaccaaatgt tgctgaagtg atgaacattg ttggtgagat tgaaggacgt
    17401 acggcaatta ttattgacga tattattgat acagcaggta caatcacttt agctgcacaa
    17461 gcattaaaag ataaaggtgc taaagaagta tatgcttgtt gtacacaccc tgttttatca
    17521 ggaccggcta aagaacgtat cgaaaattct gctataaaag aattaatcgt aacaaactca
    17581 attcatttag atgaagatcg caaaccatct aacactaaag aattatctgt tgctggttta
    17641 atcgcacaag ctatcattcg tgtatacgaa agagaatcag ttagcgtatt atttgactaa
    17701 tatttaaaag gcgtttgacg aacatattcc aaacgtgtat aatagtttcg ttcgtgatta
    17761 tacgaataaa taaacacttg caagcaacga tgatgttgat gggtaagtga ggtgctcgtt
    17821 ttgagcaaaa atgaaaggtg gaaatgagaa tggcttcatt aaagtcaatc atccgtcaag
    17881 gtaaacaaac acgttcagat cttaaacaat taagaaaatc tggtaaagta ccagcagtag
    17941 tatacggtta cggtactaaa aacgtgtcag ttaaagttga tgaagtagaa ttcatcaaag
    18001 ttatccgtga agtaggtcgt aacggtgtta tcgaattagg cgttggttct aaaactatca
    18061 aagttatggt tgcagactac caattcgatc cacttaaaaa ccaaattact cacattgact
    18121 tcttagcaat caatatgagt gaagaacgta ctgttgaagt accagttcaa ttagttggtg
    18181 aagcagtagg cgctaaagaa ggcggcgtag ttgaacaacc attattcaac ttagaagtaa
    18241 ctgctactcc agacaatatt ccagaagcaa tcgaagtaga cattactgaa ttaaacatta
    18301 acgacagctt aactgttgct gatgttaaag taactggcga cttcaaaatc gaaaacgatt
    18361 cagctgaatc agtagtaaca gtagttgctc caactgaaga accaactgaa gaagaaatcg
    18421 aagctatgga aggcgaacaa caaactgaag aaccagaagt tgttggcgaa agcaaagaag
    18481 acgaagaaaa aactgaagag taattttaat ctgttacatt aaagttttta tactttgttt
    18541 aacaagcact gtgcttattt taatataagc atggtgcttt ttgtgttatt ataaagctta
    18601 attaaacttt attactttgt actaa
//
LOCUS       DAXWFK010000038        18379 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.038, whole genome shotgun sequence.
ACCESSION   DAXWFK010000038 DAXWFK010000000
VERSION     DAXWFK010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 18379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18379
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.038"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            97..519
                     /locus_tag="ACGEXS_001031"
     CDS             97..519
                     /locus_tag="ACGEXS_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFF8012051.1"
                     /translation="MPYNYKKQNGELMSVMSQGEKFIHQSPVNDELSALIKLLISKIN
                     GCHYCVDIHKKELKELGVTQMKIDEVLSFRHLDLFTDQEKVTLEFAEMLNSIKDFKKF
                     EIIDRLKSFYDEEQIIDLVFVVNQINGWNRLNIISDRL"
     gene            complement(631..2169)
                     /locus_tag="ACGEXS_001032"
     CDS             complement(631..2169)
                     /locus_tag="ACGEXS_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFF8012052.1"
                     /translation="MTSKHQQKGSIVNRFLNSVEKIGNKLPDPSVLFFLMCVGLAIMT
                     WVISLFNVSVKHPGTHQTIYIKNIISHDGFTLIMNDTIKNFSEFPALGLVLAVMIGIG
                     VAEKTGYFDKLMISVVNRAPRFLILPTIILIGILGSTAGDAATIILPPLAAMLFIKIG
                     YHPIAGLTMAYASAVGGFAANIVVGMQDALVYSFTEPATRIVSDSIKTNVAMNWYFIA
                     ASVVVLLPTILLVTTKLIIPRLGKYDDSLMHDDHEETSSHITDKEAHALKWANISFIV
                     TIILLIITAIPEHSFLRNAKTGSLLDDAPLINGVGLIILVVFLVPGLVYGILSKEIKN
                     TKDLGKMFGDAVGSMGTFIVIVFFAAQLLAYLKWSNLGIIAAVKGAKLLEHQNGIVLI
                     LGIIVLSAMVNMLIGSASAKWGILGPIFVPMLILIGFHPAFTQVIYRVGDSITNPITP
                     MMPYLPLLLTYAQKYDKRMKLGALLSSLMPYSIALSIVWTVFVIIWFLLGIPVGPGGP
                     IFVK"
     gene            2402..3220
                     /locus_tag="ACGEXS_001033"
                     /pseudo
     CDS             2402..3220
                     /locus_tag="ACGEXS_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002503368.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            3483..3848
                     /locus_tag="ACGEXS_001034"
     CDS             3483..3848
                     /locus_tag="ACGEXS_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012053.1"
                     /translation="MLKEKESFRLLYQAIREIADKIGDNQLETNSVSLLLLDFDFEHE
                     VFDELYLVILKYLNTVSIENISHSELLNLIENTIPEDREINTFVKNKIIIGFANNYFP
                     ELQVLANEIKSDMASSLKQ"
     gene            4715..5536
                     /gene="cntK"
                     /locus_tag="ACGEXS_001035"
     CDS             4715..5536
                     /gene="cntK"
                     /locus_tag="ACGEXS_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine racemase CntK"
                     /protein_id="HFF8012054.1"
                     /translation="MNRQVIEFSKYNPSGNMTILVHSKHDASEYASIANQLMAATHVC
                     CEQVGFIESTQNDDGNDFHLVMSGNEFCGNATMSYIHHLQESHLLKDQQFKVKVSGCS
                     DLVQCAIHDCQYYEVQMPQAHRVVPTTINMGNHSWKAIEIIYETYVHYVIPVKQVTTE
                     IQHLVEAFVREQQWSHKYKTVGMMLFDEQRQFLQPLIYIPEIQSLIWENSCGSGTASI
                     GVFNNYQRNDACKDFTVHQPGGSILVTSKRCHQLGYQTSIKGQVTTVATGKAYIE"
     gene            5547..6365
                     /gene="cntL"
                     /locus_tag="ACGEXS_001036"
     CDS             5547..6365
                     /gene="cntL"
                     /locus_tag="ACGEXS_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-histidine (S)-2-aminobutanoyltransferase CntL"
                     /protein_id="HFF8012055.1"
                     /translation="MNNFNNEIKLILQQYLEKFEAHYERVLQDDQYIEALETLMDDYS
                     EFILNPIYEQQFNAWRDVEEKAQLIKSLQYITAQCVKQVEVIRARRLLDGQASTTGYF
                     DNIEHCIDEEFGQCSIASNDKLLLVGSGAYPMTLIQVAKETGASVIGIDIDPQAVDLG
                     RRIVNVLAPNEDITITDQKVSELKDIKDVTHIIFSSTIPLKYSILEELYDLTNENVVV
                     AMRFGDGIKAIFNYPSQETAEDKWQCVNKHMRPQQIFDIALYKKAAIKVGITDV"
     gene            6358..7659
                     /gene="cntM"
                     /locus_tag="ACGEXS_001037"
     CDS             6358..7659
                     /gene="cntM"
                     /locus_tag="ACGEXS_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine dehydrogenase CntM"
                     /protein_id="HFF8012056.1"
                     /translation="MSKLLMIGTGPVAIQLANICYLKSDYEIDMVGRASTSEKSKRLY
                     QAYKKEKQFEVKIQNEAHQHLEGKFEINRLYKDVKNVKGEYETVVMACTADAYYDTLQ
                     QLSLETLQSVKHVILISPTFGSQMIVEQFMSKFNKDIEVISFSTYLGDTRIVDKEAPN
                     HVLTTGVKKKLYMGSTHSNSTMCQRISALAEQLKIQLEVVESPLHAETRNSSLYVHPP
                     LFMNDFSLKAIFEGTDVPVYVYKLFPEGPITMTLIREMRLMWKEMMVILQAFRVPSVN
                     LLQFMVKENYPVRPETLDEGDIEHFEILPDILQEYLLYVRYTAILIDPFSQPDENGHY
                     FDFSAVPFKQVYKNEQDVVQIPRMPSEDYYRTAMIQHIGKMLGIKTPMIDQFLTRYEA
                     SCQAYKDMHQDQHLSSQFNTNLFEGDKALVTKFLEINRTLS"
     gene            7802..9400
                     /gene="cntA"
                     /locus_tag="ACGEXS_001038"
     CDS             7802..9400
                     /gene="cntA"
                     /locus_tag="ACGEXS_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine-dependent metal ABC transporter
                     substrate-binding protein CntA"
                     /protein_id="HFF8012057.1"
                     /translation="MRKLTKMSAMLLASGLILTGCGGNKGLEEKKENKQLTYTTVKDI
                     GDMNPHVYGGSMSAESMIYEPLVRNTKDGIKPLLAKKWDVSEDGKTYTFHLRDDVKFH
                     DGTPFDADAVKKNIDAVQENKKLHSWLKISTLIDNVKVKDKYTVELNLKEAYQPALAE
                     LAMPRPYVFVSPKDFKNGTTKDGVKKFDGTGPFKLGEHKKDESADFNKNDQYWGEKSK
                     LNKVQAKVMPAGETAFLSMKKGETNFAFTDDRGTDSLDKDSLKQLKDTGDYQVKRSQP
                     MNTKMLVVNSGKKDNAVSDKTVRQAIGHMVNRDKIAKEILDGQEKPATQLFAKNVTDI
                     NFDMPTRKYDLKKAESLLDEAGWKKGKDSDVRQKDGKNLEMAMYYDKGSSSQKEQAEY
                     LQAEFKKMGIKLNINGETSDKIAERRTSGDYDLMFNQTWGLLYDPQSTIAAFKAKNGY
                     ESATSGIENKDKIYNSIDDAFKIQNGKERSDAYKNILKQIDDEGIFIPISHGSMTVVA
                     PKDLEKVSFTQSQYELPFNEMQYK"
     gene            9413..10348
                     /gene="opp1B"
                     /locus_tag="ACGEXS_001039"
     CDS             9413..10348
                     /gene="opp1B"
                     /locus_tag="ACGEXS_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt ABC transporter permease"
                     /protein_id="HFF8012058.1"
                     /translation="MFKFILKRIALMFPLMIVVSFMTFLLTYITNENPAVTILHAQGT
                     PNVTPELIAETNEKYGFNDPLLIQYKNWLLEAMQFNFGTSYITGDPVAERIGPAFMNT
                     LKLTIISSVMVMITSIILGVVSALKRGKFTDRAIRSVAFFLTALPSYWIASILIIYVS
                     VKLNILPTSGLTGPESYILPVIVITIAYAGIYFRNVRRSMVEQLNEDYVLYLRASGVK
                     SITLMLHVLRNALQVAVSIFCMSIPMIMGGLVVIEYIFAWPGLGQLSLKAILEHDFPV
                     IQAYVLIVAVLFIVFNTLADIINALLNPRLREGAR"
     gene            10345..11214
                     /gene="opp1C"
                     /locus_tag="ACGEXS_001040"
     CDS             10345..11214
                     /gene="opp1C"
                     /locus_tag="ACGEXS_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt ABC transporter permease"
                     /protein_id="HFF8012059.1"
                     /translation="MIILKRLLQDKGAVIALGIIVLYVFLGLAAPLVTFYDPNHIDTA
                     NKFAGMSFQHLLGTDHLGRDILTRLIYAIRPSLLYVFVALFVSVLIGSILGFLSGYFQ
                     GFVDALIMRACDVMLAFPSYVVTLALIALFGMGAENIIMAFILTRWAWFCRVIRTSVM
                     QYTASDHVRFAKTIGMNDMKIIHKHIMPLTLADIAIISSSSMCSMILQISGFSFLGLG
                     VKAPTAEWGMMLNEARKVMFTHPEMMFAPGIAIVIIVMAFNFLSDALQIAIDPRISSK
                     DKLRSVKKGVVQS"
     gene            11211..12026
                     /locus_tag="ACGEXS_001041"
     CDS             11211..12026
                     /locus_tag="ACGEXS_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8012060.1"
                     /translation="MTLLTVKHLTITDTWTDQPLVSDVNFTLTKGETLGVIGESGSGK
                     SITCKSIIGLNPERLGVTGEIIFDGTSMLSLSESQLKKYRGKDIAMVMQQGSRAFDPS
                     TTVGKQMFETMKVHTSMSTQEIEKTLIEYMDYLSLKDPKRILKSYPYMLSGGMLQRLM
                     IALALALKPKLIIADEPTTALDTITQYDVLEAFIDIKKHFDCAMIFISHDLTVINKIA
                     DRVVVMKNGQLIEQGTRESVLHHPEHVYTKYLLSTKKKINDHFKHVMRGDVHD"
     gene            12019..12768
                     /locus_tag="ACGEXS_001042"
     CDS             12019..12768
                     /locus_tag="ACGEXS_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8012061.1"
                     /translation="MIKIKDVEKSYQSAHVFKRRRTPIVKGVSFECPIGATIAIIGES
                     GSGKSTLSRMILGIEKPDKGCVTLNDQPMHKKKVRRHQIGAVFQDYTSSLHPFQTVRE
                     ILFEVMCQCDGQPKEVMEVQAITLLEEVGLSKAYMDKYPNMLSGGEAQRVAIARAICI
                     NPKYILFDEAIGSLDMSIQTQILDLLIHLRETRQLSYIFITHDIQAATYLCDQLIIFK
                     NGKIEEQIPTSALHKSDNAYTRELIEKQLSF"
     gene            12780..13973
                     /locus_tag="ACGEXS_001043"
     CDS             12780..13973
                     /locus_tag="ACGEXS_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8012062.1"
                     /translation="MKGAMAWPFLRLYILTLMFFSANAILNVFIPLRGHDLGATNTVI
                     GIVMGAYMLTAMVFRPWAGQIIARVGPIKVLRIILIINAIALIIYGFTGLEGYFVARV
                     MQGVCTAFFSMSLQLGIIDALPEEHRSEGVSLYSLFSTIPNLIGPLVAVGIWNANNIS
                     LFAIVIIFIALTTTFFGYRVTFAEQEPDTSDKIEKMPFNAVTVFAQFFKNKELLNSGI
                     IMIVASIVFGAVSTFVPLYTVSLGFANAGIFLTIQAIAVVAARFYLRKYIPSDGMWHP
                     KYMVSVLSLLVIASFVVAFGPQVGAIIFYGSAILIGMTQAMVYPTLTSYLSFVLPKVG
                     RNMLLGLFIACADLGISLGGALMGPISDLVGFKWMYLICGMLVIVIMIMSFLKKPTPR
                     PASSL"
     gene            complement(14197..14946)
                     /gene="lnsB"
                     /locus_tag="ACGEXS_001044"
     CDS             complement(14197..14946)
                     /gene="lnsB"
                     /locus_tag="ACGEXS_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family lipoprotein N-acylation protein
                     LnsB"
                     /protein_id="HFF8012063.1"
                     /translation="MSNTNKHYIEEEYATEQSRFFKRDIGFIFLYIFLVNILPIIIVF
                     LTFGLVAVFTQQAPWGFSFPISMNISYLLAVIISLAIFRLMHEDAFIPMIRSQIVASA
                     KYKWHILISFTLSVVLFLLLNHFNIFALNVDSFTVFDIYPLIKGYGSISLIAIFSLTI
                     ILNTLICQLIFRHILIQELSRLIPLKITIVLSIILETLFYYPYINTWWEFIPALILAG
                     SATYLYLKSYRNFMVSYFYQLAVILVLHIFM"
     gene            15141..16718
                     /locus_tag="ACGEXS_001045"
     CDS             15141..16718
                     /locus_tag="ACGEXS_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HFF8012064.1"
                     /translation="MTFLTVMQFIVNIIVVGFMLTVIVIGLIWLIKDKRQSQHSVLRN
                     YPLLARIRYISEKMGPELRQYLFSGDNEGKPFSRNDYKNIVLAGKYNSRMTSFGTTKD
                     YQDGFYIQNTMFPMQRNEISVDNTTLLSTFIYKIANERLFSREEYRVPTKIDPYYLSD
                     DHAIKLGEHLKHPFILKRIVGQSGMSYGALGKNAITALSKGLAKAGTWMNTGEGGLSE
                     YHLKGNGDIIFQIGPGLFGVRDKEGNFSEGLFKEVAQLSNVRAFELKLAQGAKTRGGH
                     MEAEKVNEEIAKIRNVEPYKTINSPNRYEFIHNAEDLIRFVDQLQQLGQKPVGFKIVV
                     SKVSEIETLVRTMVELDKYPSFITIDGGEGGTGATFQELQDGVGLPLFTALPIVSGML
                     EKYGIRDKVKLAASGKLVTPDKIAIALGLGADFVNIARGMMISVGCIMSQQCHMNTCP
                     VGVATTDAKKEKALIVGEKQYRVTNYVTSLHEGLFNIAAAVGVSSPTEITADHIVYRK
                     VDGELQTIHDYKLKLIS"
     gene            complement(16886..17341)
                     /locus_tag="ACGEXS_001046"
     CDS             complement(16886..17341)
                     /locus_tag="ACGEXS_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1307 domain-containing protein"
                     /protein_id="HFF8012065.1"
                     /translation="MKKLCSLIVVALVCIIALSACGKEQTKTYEGDVSGKHVLTSITY
                     KDDKVLKQSTINTIKYDDLGMDKDEAKKLFAKSESIFKDLKGVKYKVDYKDKKAIEHL
                     DIDYTEVDMKKLNKRLGVSTKENKDISFEKLEKQLKHRGLKEKDKMDDK"
     gene            17699..17956
                     /locus_tag="ACGEXS_001047"
     CDS             17699..17956
                     /locus_tag="ACGEXS_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFF8012066.1"
                     /translation="MIITSPTEARKDFYQLLKNVNNNHEPIYISGNNAENNAVIIGLE
                     DWKSIQETIYLESTGTMDKVREREKDNSGTTNIDDIDWDNL"
     gene            17956..18222
                     /locus_tag="ACGEXS_001048"
     CDS             17956..18222
                     /locus_tag="ACGEXS_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFF8012067.1"
                     /translation="MSNYTVKIKNSAKSDLKKIKHSYLKKSFLEIVETLKNDPYKITQ
                     SFEKLEPKYLERYSRRINHQHRVVYTVDDRNKEVLILSAWSHYD"
BASE COUNT         6353 a         2700 c         3548 g         5778 t
ORIGIN      
        1 gacaagatgt tatacagaat ttcaaattct atccaatatt gttcgaagtg tagtatcact
       61 ggattggtat taaacaatgt aaaggagaga ttgcaaatgc cgtataatta caagaaacaa
      121 aatggagagt taatgtctgt aatgagccaa ggtgaaaagt ttattcatca atcacccgtt
      181 aatgatgaac ttagtgcatt gattaagtta ttaatttcta aaattaacgg ttgtcattat
      241 tgtgttgata tccataaaaa agaattaaag gaattgggtg taacacaaat gaaaattgat
      301 gaagtcttga gttttagaca tttagattta tttactgatc aagaaaaagt gacgcttgaa
      361 tttgcagaaa tgttaaattc aatcaaagac tttaagaagt ttgaaattat tgaccggcta
      421 aaatcatttt atgatgaaga acaaattatt gatcttgtct ttgttgtaaa ccaaattaac
      481 ggttggaaca gattaaatat tattagtgat agactataat tgttcatata aatgcagagt
      541 ttcatctcga acgctatatc ataacaaatc atgccactat acaggtcaaa tcttgtatag
      601 tggcatttta atttatccct ttgaatactg ttatttaacg aatatcggtc cacctggtcc
      661 aacagggata cctaatagga accaaatgat gacaaatact gtccatacaa tacttagcgc
      721 gattgaatac ggcattaaac tagaaagtaa ggctccgagt ttcatgcgtt tatcgtattt
      781 ttgtgcataa gttaataata aaggtaagta cggcatcatc ggtgtaattg gattggtaat
      841 tgaatcgcct acacggtaaa tgacttgtgt gaatgcggga tgaaagccga taaggattaa
      901 cattggtacg aatatcggtc ctaaaatacc ccatttagcc gatgcgcttc cgattaacat
      961 gttgaccatt gcactcagta caataatacc tagtatcaat acaataccgt tttgatgttc
     1021 taataatttg gcacctttaa cagcagcgat aattcctaaa ttactccact ttaaatacgc
     1081 aagtagctgt gctgcaaaaa acacaataac gataaatgtt cccattgatc ctacagcatc
     1141 gccgaacatt ttacctaagt cttttgtatt tttaatttct ttgcttaaaa tcccataaac
     1201 taatccaggt actaaaaata cgacaagaat aattaatccg acaccgttaa ttaatggcgc
     1261 atcgtctagt aagctgcctg ttttagcatt tcttaaaaag ctatgttcag gaatggctgt
     1321 aataattaat aaaataattg tgactatgaa actgatattt gcccatttta aagcatgtgc
     1381 ttctttgtca gtaatatgtg aagatgtttc ttcatggtca tcatgcatta aactgtcatc
     1441 atattttcct aatctaggaa taatgagctt agttgtaact agcaatatcg taggaagtaa
     1501 tacaacgaca ctcgctgcga taaagtacca gttcatagca acgtttgttt taatagaatc
     1561 tgaaacgata cgtgttgccg gttctgtaaa tgaatagacc aaagcatctt gcataccaac
     1621 aactatattt gctgcaaatc ctccaacagc ggaagcatat gccatcgtta gtccagcgat
     1681 agggtgatag ccaattttaa taaaaagcat tgctgcaagc ggcggcaaga taattgtcgc
     1741 agcatcgccg gctgtactac ctaaaatacc aattaatata atagtcggta aaattaagaa
     1801 acgtggtgcg cgattcacaa cagaaatcat taacttatcg aagtatcctg ttttctctgc
     1861 aacaccaata ccaatcatca ctgctagtac taagcctaat gctgggaact ctgagaaatt
     1921 tttaatcgta tcattcatta tcagcgtaaa tccatcatgg ctaattatat ttttaatata
     1981 aatggtttga tgcgtacctg gatgcttaac agatacatta aataatgaga taacccatgt
     2041 cataatggct aagcccacac acattaaaaa gaataagacg ctaggatctg gcaatttatt
     2101 tccgattttt tcaacactat tcaagaaacg attgacgata gacccctttt gttgatgttt
     2161 tgatgtcatc aattattccc ccctttgtta aatatttaaa gtgtaacaaa aaatactctc
     2221 aaaagtaaca attttcagaa aataaaaaaa ctaatattgt taaatatttt gagttattca
     2281 atagaaagtg tatagcagag tagttaagac tgcctgaaga cttatctatt aggtttatga
     2341 agcatcgaac agtggaaaat aaggactgta agtttaagat atgttgtata ggagtgactg
     2401 aatgaaacgt ttggaaaata aagtagcagt cgtaacagga gcaagtacag gtatcggtca
     2461 agcttctgca atcgctttag ctcaataagg tgcgtatgta ttggcggtag acatagctga
     2521 agcggtatcg gagactgtcg ataaaattaa aagtaatggt gacaatgcga aggcgtataa
     2581 tgtggataat gcaagcgaac aacaagtggt agactttgtg tctgacataa aggaacagtt
     2641 tggaagaatc gatgtgttgt ttaataatgc cggtgtggat aatgcggctg atagaattca
     2701 tgagtatcca atagatgtgt atgacaagat tatgaatgta gatatgcgtg ggacattttt
     2761 aatgacgaaa atgatgttac ctttaatgat gaatcaaggt ggctctattg ttaatacgtc
     2821 ttcattttcc ggacaagcag cagacttgta tcgctctgga tataatgctg cgaaaggtgc
     2881 agtgattaat tttacaaaat caatcgcaat tgagtatggc cgtgatagca ttcgagccaa
     2941 tgcgattgca ccaggtacaa ttgaaacccc gttagtagat aaactgacag gtacgagtga
     3001 ggatgatgca ggtaaaacat ttagagaaaa tcaaaaatgg atgactccgc tgggacgttt
     3061 aggtaaacca gaagaagttg ctaaattagt agtcttctta gcatctgacg acagttcatt
     3121 cataactgga gagacgattc gaattgatgg tggtgtgatg gcttacacat ggcctggcga
     3181 gatgttaagt gatgattcgt ggaagcggac attggaataa ttatttaaaa tcgatatcca
     3241 gtggaaaaga atttggcatg aagtaggcaa tgtatgcata ttgtcgcaat gaaagacatt
     3301 taaagcggag attaattaat ctttgaaaat cacatattgt tcatttgaag tgtattgctt
     3361 aaagcttgtt ttaacgtatt gtaggtcgtg catctaattt atcagaatct cgagttcaaa
     3421 cttttggagt atctttaaaa taagctgtat tttgtctatt ctaataaatt aaggagaatt
     3481 ttatgttaaa agaaaaagaa agttttagat tgctatatca agctataaga gagattgcag
     3541 ataaaattgg agataatcag ttagaaacta attccgttag tttattatta ttggactttg
     3601 attttgaaca tgaagtattt gatgaattgt atcttgtgat tttaaaatat ttaaatacag
     3661 taagtataga gaacataagt catagtgagc ttttgaattt aatagaaaat acgattccag
     3721 aagatcgaga gattaacacg tttgtaaaaa ataaaataat aattggcttt gctaataatt
     3781 acttccctga attacaagta ttagcaaacg aaataaaatc tgatatggct agttcattaa
     3841 aacaatgata tttttattta aatttttaaa gctttgtacg aaattgtaca aagctttttt
     3901 ggtgcgtatt gtatgggcaa caacttgacg atgaaaatcc gttacaggat tggtaatagg
     3961 aaatgttagc gaaagacaag ggtatccatt gtagattaac aaaaggacgt ttccacaagt
     4021 gtgggttatt ctcactaaag caatacgcag agacaactta cgtaaaattt tgaactgact
     4081 agaacggaac ttctactcaa ttattgataa aaattttcaa aaagacttga atgtgctgag
     4141 aatacgaagt ttatggaagg attatcaaaa tataaatgtg cattcattta caacctttat
     4201 tgacaatgat tctcaactaa tatagtatat aatcaaatcg taatagttac gatttgtttt
     4261 ctgcaacttt tttgaagttt tagttgaggt gaaaacagta aaagcatcta agtgaatgta
     4321 gttaacggac aactgcattc gcttgtagag ccacaagaag caactttaaa taaggtttac
     4381 ggttgcattt tgatacaaca accgattact aagtcatgct ttccactttg cgggttagca
     4441 tgacttacct aatagataga gctattaggt tcagcttcta aaaaattaca gttttagagg
     4501 aatacagttg cttgcttcgc aacaactgca taagagccat ggttttcgct tttgcgaatt
     4561 agcatgactt acctactaga tagagctatt aggttcatct tctaaaaaat tacaggttta
     4621 gaggaataca gttgtttgct tcgcaacaac tgcataagag cctctagtaa ttaaaattac
     4681 agaggctcta aaaatacatc taaaggagtg tcgtatgaat cggcaggtta tagaattttc
     4741 taagtataat ccttcgggga atatgacgat acttgttcat tcaaaacatg atgctagtga
     4801 atatgcatct atcgccaatc agttgatggc cgcaacacat gtatgctgtg aacaggtagg
     4861 ctttatagaa tcaacacaaa atgatgatgg taatgatttt cacttagtta tgagcggtaa
     4921 tgaattttgc ggtaatgcga cgatgtcata tatacatcat ttgcaggaaa gtcatttgct
     4981 taaagaccaa cagtttaagg tgaaggtgtc tggctgttcg gatttagtgc aatgcgcaat
     5041 tcatgattgc caatactatg aagttcaaat gccacaagcc catcgtgttg tgccaacaac
     5101 aattaatatg ggtaatcatt catggaaagc aatagaaatt atttatgaaa catatgtaca
     5161 ttatgtgatt ccagttaaac aagtaacaac agaaattcaa catttggttg aagcgtttgt
     5221 gcgtgagcaa caatggagtc acaaatataa aacagtaggt atgatgcttt ttgatgaaca
     5281 acgtcaattt ttacagccat taatctatat accagaaatt caaagtttaa tttgggaaaa
     5341 tagctgtggt tctggtacag catcaattgg ggtttttaat aattatcaac gtaatgacgc
     5401 atgcaaagat tttacagtac atcagccagg gggcagtatt ttagtgacat caaagcgatg
     5461 tcatcaattg ggatatcaaa cttcaattaa aggacaggtt acaactgtag ctacaggaaa
     5521 agcatatata gaataaggag cctacaatga ataactttaa taatgaaatc aaattgatat
     5581 tacaacaata tttagaaaag tttgaagcgc attacgagcg tgtattacaa gacgatcaat
     5641 atatcgaagc attagaaaca ttgatggatg actatagtga atttatttta aatcctattt
     5701 atgaacaaca atttaatgct tggcgtgacg ttgaagaaaa agcacaatta ataaaatcac
     5761 tgcaatatat tacagcgcag tgtgttaaac aagtggaagt cattagagcg agacgtctat
     5821 tagacggaca ggcgtctacc acaggttact ttgacaatat agaacattgt attgatgaag
     5881 agtttggaca atgtagtata gctagcaatg acaaattatt gttagttggt tcaggtgcat
     5941 atccaatgac gttaattcaa gtagcaaaag aaacaggtgc ttcagttatc ggtattgata
     6001 ttgatccaca agccgttgac ctagggcgca gaatcgttaa cgtcttagca ccaaatgaag
     6061 atataacaat tacggatcaa aaggtatctg aacttaaaga tatcaaagat gtgacgcata
     6121 tcatattcag ctcgacaatt cctttaaagt acagcatttt agaagaatta tatgatttaa
     6181 caaatgaaaa tgtcgtagtt gcaatgcgct ttggtgatgg catcaaagca atatttaatt
     6241 atccgtcaca agaaacagcg gaagataagt ggcaatgtgt gaataaacat atgagaccac
     6301 agcaaatttt tgatatagca ctttataaaa aagcagctat aaaggtaggt attacggatg
     6361 tctaaattat taatgatagg cactggtccg gtcgcaatcc aattagcgaa tatttgctat
     6421 ttaaaatcag attatgagat tgatatggtt ggacgtgcct caacatcaga aaaatcaaaa
     6481 cgcttatatc aagcgtataa aaaagagaaa caatttgaag tcaaaataca aaacgaggcg
     6541 catcaacatc tggaaggtaa gtttgaaatt aatcgtttgt ataaagatgt taaaaacgtt
     6601 aagggtgaat acgaaacggt tgtcatggca tgcacagcag atgcttatta tgacacacta
     6661 cagcaattgt cgttagaaac tttgcaaagt gtcaaacatg tcattttaat atcaccgaca
     6721 tttggttcgc aaatgattgt cgaacaattt atgtctaaat ttaataaaga tatcgaagtg
     6781 atttcattct caacttatct tggcgataca cgtattgttg ataaagaagc gcctaatcat
     6841 gtgttgacaa caggtgtaaa aaagaaattg tacatgggat cgacacattc aaactcaaca
     6901 atgtgtcaac gaatctctgc tttagctgag caattgaaga ttcaattaga agtcgttgaa
     6961 tcgccactgc atgctgaaac gcgaaacagt tcgctttatg tgcacccacc actatttatg
     7021 aatgactttt cattgaaagc cattttcgaa ggaacagatg taccggttta tgtgtataag
     7081 ttatttcctg aaggaccgat aacgatgaca ctaatccgtg aaatgcgttt aatgtggaag
     7141 gaaatgatgg ttattttaca agcatttaga gtgccgtcag tcaacctgct tcaatttatg
     7201 gtgaaggaaa attatccagt acgtcctgaa actttggatg aaggtgatat tgagcatttc
     7261 gaaatcttgc cagatatctt acaagaatat ctgctttatg taagatatac cgcaatcctc
     7321 attgatccat tttcacagcc agacgaaaac ggacattact ttgatttttc agctgtacca
     7381 tttaagcaag tctataaaaa tgaacaggat gttgttcaaa ttccaagaat gccaagtgaa
     7441 gattattaca gaacggcgat gattcagcat attgggaaaa tgctaggtat caaaacgcca
     7501 atgattgatc agttcctaac tcgctatgaa gcaagttgcc aggcgtacaa ggatatgcat
     7561 caagatcaac acttatcttc tcaatttaat acaaatctat ttgaaggaga taaagcactc
     7621 gtcacaaaat ttttggaaat caatagaacg ctttcataat aagggtttga agttttataa
     7681 tagaaaaaaa ttattgaatt atgtttgaca tttacataaa aataagcaaa taattgagaa
     7741 aaataatcat tacgatttga ttaagtaatg caacttatca atttagaaag aggaaaagca
     7801 aatgagaaaa ctaactaaaa tgagtgcaat gttacttgca tcagggctaa ttttaactgg
     7861 ttgtggcggt aataaaggtt tagaggagaa aaaagaaaac aagcaattaa cgtatacgac
     7921 ggttaaagat atcggtgata tgaatccgca tgtttacggt ggatcaatgt ctgctgaaag
     7981 tatgatatac gagccgcttg tacgtaacac gaaagatggt attaagcctt tactagctaa
     8041 aaagtgggat gtgtctgaag atgggaagac atacacgttc catttgagag atgacgttaa
     8101 attccatgat ggtacgccat ttgatgctga cgcagttaag aaaaatattg acgcagttca
     8161 agaaaacaaa aaattgcatt cttggttaaa gatttcgaca ttaattgaca atgttaaagt
     8221 taaagataag tacacggttg aattgaattt gaaagaagca tatcaacctg cattggctga
     8281 attagcgatg cctcgtccat atgtatttgt gtctccaaaa gactttaaaa acggtacaac
     8341 aaaagatggc gttaaaaagt tcgatggtac tggtccattt aaattaggtg aacacaaaaa
     8401 agatgagtct gcagacttta acaaaaatga tcaatactgg ggcgaaaagt ctaaacttaa
     8461 caaagtacaa gcaaaagtaa tgcctgctgg tgaaacagca ttcctatcaa tgaaaaaagg
     8521 tgaaacgaac tttgccttca cagatgatag aggtacagat agcttagaca aagactcttt
     8581 aaaacaattg aaagatacag gtgactatca agttaagcgt agtcaaccta tgaatacgaa
     8641 aatgttagtt gtcaattctg gtaaaaaaga taacgctgtg agtgacaaaa cagtcagaca
     8701 agcgattggt catatggtaa acagagataa aattgccaaa gaaattttag atggtcaaga
     8761 aaaaccagca actcaattat ttgcgaaaaa tgtaacagac attaatttcg atatgccaac
     8821 acgtaagtat gaccttaaaa aagcagaatc attattagat gaagctggtt ggaagaaagg
     8881 taaagacagc gatgttcgtc aaaaagatgg taaaaacctt gaaatggcaa tgtactatga
     8941 caaaggttct tcaagtcaaa aagaacaagc agaatactta caagcagaat ttaagaaaat
     9001 gggtattaag ttaaacatca atggcgaaac atcagataaa attgctgaac gtcgtacttc
     9061 tggtgattat gacttaatgt tcaaccaaac ttggggatta ttgtacgatc cacaaagtac
     9121 tattgcagca tttaaagcga aaaatggtta tgaaagtgca acatcaggca ttgagaacaa
     9181 agataaaata tacaacagca ttgatgacgc atttaaaatc caaaacggta aagagcgttc
     9241 agacgcttat aaaaacattt tgaaacaaat tgatgatgaa ggtatcttta tccctatttc
     9301 acacggtagt atgacagttg ttgcaccaaa agatttagaa aaagtatcat tcacacaatc
     9361 acagtatgaa ttaccattca atgaaatgca gtataaataa aggagcaatt agatgttcaa
     9421 atttatctta aaacgtattg cgctcatgtt tccattgatg attgtagtaa gttttatgac
     9481 atttctattg acgtatatta caaatgaaaa tccagctgtg acaattttac atgcacaagg
     9541 gacgccaaat gtaacaccag agttgattgc agaaacgaat gagaagtacg gtttcaatga
     9601 tccattatta attcaatata aaaattggtt acttgaagcg atgcaattta attttggtac
     9661 aagctacatt acaggtgacc cagttgctga acgtattggt ccagcattta tgaatacatt
     9721 gaaattaaca ataatttcaa gtgttatggt gatgattaca tcaattattt taggtgtagt
     9781 tagtgcatta aaaagaggaa agttcactga tcgtgcgata cgttcagtgg ctttctttct
     9841 aactgcatta ccatcatatt ggatagcttc aatacttatt atttacgttt cagtgaagtt
     9901 aaacatattg ccgacttctg gattaacagg tccagaaagt tacatattgc cagtgatcgt
     9961 tattacgatt gcctatgctg gtatttactt tagaaatgtt agacgctcga tggtggaaca
    10021 attaaatgaa gattatgtac tttatttaag agcaagcggt gtgaaatcta tcacattaat
    10081 gttgcatgtg ttgcgtaatg ctttacaagt tgcggtatca atcttttgta tgtctatacc
    10141 aatgataatg ggtggactag ttgttatcga gtatatcttt gcatggcctg gactaggtca
    10201 attaagttta aaagcaatac ttgaacacga ttttccagtc attcaagcat atgtattaat
    10261 tgtagcggta ttatttattg tatttaatac attagcagat atcattaatg cgctattaaa
    10321 tccaagatta agggagggcg cacgatgata attttaaaac gattattaca agataaaggt
    10381 gcagtaattg ctttaggcat tattgtatta tatgtctttt taggattagc agcaccactt
    10441 gtgacatttt atgatcctaa ccatatcgat acagcaaaca aatttgctgg catgagtttt
    10501 caacatctac taggtactga ccatttaggt agagatattt taactaggtt aatttatgcg
    10561 attagaccaa gtttgttata tgtctttgtt gcgctatttg tttctgtact tattggatct
    10621 attttaggat tcttatcagg atatttccaa gggtttgttg acgccttaat catgcgtgcg
    10681 tgtgatgtta tgttggcatt cccaagttat gttgtaacgt tagcattaat tgcattgttt
    10741 ggaatgggtg ccgaaaatat tatcatggca tttattttga cgcgttgggc atggttctgt
    10801 cgtgttatac gtacaagtgt tatgcagtac actgcttctg accatgtaag atttgctaaa
    10861 acaatcggta tgaatgatat gaaaattatt cacaaacata ttatgccatt aacattagca
    10921 gatattgcta tcatctctag tagctcgatg tgttcaatga tcttgcaaat atctggcttt
    10981 tcatttttag gattaggtgt caaagcgcct actgcagagt ggggcatgat gcttaacgaa
    11041 gctagaaaag tgatgtttac acatcctgaa atgatgtttg cgccaggtat tgccatagtg
    11101 attatagtga tggcatttaa cttcttatcc gatgctttac aaattgctat tgatccccgc
    11161 atctcttcta aagataaact tcgttctgtg aaaaaaggag tggtgcaatc atgacattgt
    11221 taacagttaa acatttgacg attacagata cctggacaga tcaaccactc gtgagtgatg
    11281 tgaattttac attaactaag ggtgaaactt taggcgttat tggagaaagt ggtagtggta
    11341 aatcaatcac ttgtaaatcg attattggtt tgaatcccga acgactcggg gtgacaggtg
    11401 aaattatctt tgatggtaca tcaatgttgt cattatctga atcgcaattg aaaaagtacc
    11461 gtggtaaaga cattgcgatg gtcatgcaac aaggtagtcg tgcctttgac ccatcaacta
    11521 ctgtcggtaa acaaatgttt gagactatga aagtacatac gtcaatgtct acacaagaaa
    11581 ttgaaaagac attgattgaa tatatggatt atttaagttt gaaagatcct aaacgtatat
    11641 taaaatcata cccttacatg ttatcaggag gaatgttaca gcgattgatg attgctttag
    11701 cgttagcttt gaaaccaaag ttaatcattg ctgatgagcc gacaacggct ttagatacaa
    11761 ttacacaata tgatgtactg gaagcattta tagatattaa aaaacacttt gactgtgcga
    11821 tgattttcat ttcacatgat ttaacggtta ttaacaagat tgcagaccgt gttgttgtga
    11881 tgaaaaatgg tcagcttatt gaacaaggga cacgtgaatc agtcttgcat catccagaac
    11941 atgtttatac gaagtattta ttatcaacga agaagaagat taatgatcat tttaaacatg
    12001 tgatgagggg tgatgtacat gattaaaatt aaagatgttg aaaagtcata tcaaagcgca
    12061 catgttttta agcgtcgtcg aacacctatc gtgaaaggtg tgtcatttga gtgtccaatc
    12121 ggtgcgacga ttgcgattat cggagaaagt ggtagcggta aatcgacgtt gagtcgtatg
    12181 atattaggta ttgagaaacc ggataaaggt tgtgtaacct taaatgatca accgatgcat
    12241 aagaagaaag tgagacgtca tcaaattggt gctgtatttc aagattatac gtcatcatta
    12301 catccatttc agactgttag agaaatctta tttgaagtga tgtgtcaatg tgatggacaa
    12361 cctaaagaag ttatggaagt ccaagcaatt acattgttgg aagaagtcgg tctatctaag
    12421 gcatacatgg ataaatatcc taatatgtta tcaggtggag aggcgcaacg tgttgcgatt
    12481 gcgcgtgcaa tatgtattaa ccctaaatat attttgtttg atgaagccat tggttcactc
    12541 gacatgtcaa ttcaaacaca aatattagat ttattgattc atttacgtga aacgcgtcag
    12601 ttgagttata tttttatcac acatgatatt caagctgcca cgtatttatg tgatcaatta
    12661 attattttta aaaacggaaa aatagaagaa caaattccga caagcgcatt gcataaaagt
    12721 gacaatgctt atacaagaga attaatagaa aaacaactat cattctaagg agtgagataa
    12781 tgaaaggtgc aatggcttgg ccctttttga gattatatat attaacattg atgttcttta
    12841 gtgccaatgc aatcttaaac gtgtttatac ctttacgagg gcatgattta ggcgcaacga
    12901 atacggttat cggtatcgtt atgggggcat acatgttaac agcaatggta tttcgaccat
    12961 gggcaggaca aattattgct cgtgtcggtc ccattaaagt attaagaatt attttgatta
    13021 tcaatgccat agctttaatt atttatggtt ttactggctt agaaggttat ttcgtagcac
    13081 gtgttatgca aggtgtgtgt acggcattct tttctatgtc tttacagcta ggtattattg
    13141 atgcattacc agaggaacat cgttctgaag gtgtatcatt gtactcgcta ttttcaacga
    13201 ttccaaactt aatcggacca ttagttgccg taggtatttg gaatgcaaat aatatttcac
    13261 tatttgcaat tgtcattatc tttatcgcat taacaacaac attctttggc tatcgcgtga
    13321 cctttgctga acaggaaccc gatacgtcag ataagattga aaaaatgccg tttaacgctg
    13381 taactgtttt tgcgcaattt ttcaaaaata aagagttgtt gaacagtggt attatcatga
    13441 ttgttgcatc gattgtattt ggtgcagtta gtacatttgt accgttatac acagtgagtt
    13501 taggattcgc gaatgcggga atctttttga caatacaggc catcgcagtt gttgcggcaa
    13561 gattttactt aaggaaatac attccgtcag atggtatgtg gcatcctaaa tatatggtat
    13621 ctgtactatc attattagta atcgcgtcat ttgtagtggc atttggtccg caagtaggtg
    13681 caattatttt ctatggtagt gcgatattaa taggaatgac gcaagcaatg gtgtacccaa
    13741 cattaacatc atacttaagc ttcgtcttac caaaagtagg tcgtaatatg ttgttaggtt
    13801 tatttattgc ctgtgcagac ttaggtatat cgttaggtgg cgcattgatg ggacctattt
    13861 ccgatttagt aggatttaaa tggatgtatc taatttgtgg tatgttagtc attgtaataa
    13921 tgattatgag tttcttgaaa aagccaacac cacgtccagc gagtagtctt taatgaagtg
    13981 aattaaagca tattaagtta atgaatattt aaattttaaa aggtatattg agcatggcga
    14041 ttcatgtgct tcatgctagg acatgaaaca ttctatatgg ctcgttttta gaacgacagt
    14101 atatctaaat aaagcacgct taaaagtgag ttttgatact gacttttaag tgtgcttttt
    14161 gatgtgtatg gataatggat aatactctga aaaattttac atgaaaatat gcaaaacgag
    14221 tataactgct aattgataga aatagctcac cataaaatta cggtatgatt ttaaatataa
    14281 gtaagtcgca ctacctgcta gtatcaatgc tggaatgaat tcccaccatg tattaatgta
    14341 tggatagtag aacagagttt caaggataat ggacaatact attgtaatct ttaaaggtat
    14401 taatctgctt aattcttgaa ttaaaatatg acggaaaata agttgacaaa tcaaagtatt
    14461 taatataatg gttaacgaaa atatagctat taaactgatg gaaccatacc ctttaatgag
    14521 cgggtaaatg tcaaagacag taaaggaatc tacatttagt gcgaaaatat tgaaatgatt
    14581 taaaagtaaa aagagtacga cacttagtgt aaatgatata agaatatgcc atttatattt
    14641 agcactagca acgatttgcg aacgtatcat tggaataaac gcatcttcat gcatcagacg
    14701 aaaaatagct agtgaaataa taactgcgag taaatagcta atgttcattg aaataggaaa
    14761 agagaaaccc cacggagctt gttgagtgaa tacagctact aacccaaaag ttaaaaagac
    14821 gataatgatc ggcaagatgt taaccaaaaa tatgtaaagg aaaataaatc caatatcacg
    14881 tttgaaaaaa cgcgattgtt cggtagcgta ttcttcttct atgtaatgtt tatttgtatt
    14941 tgacatagta tacctcttaa atagttgtat tatatagata ctttagcaca tattactttg
    15001 tattgtatgt tttatacatt aaaatttaaa atgaaaaaca tatcataaaa ttgttttata
    15061 aaatgaagcg cttccattgt gttttgtttt gtaaggtgta tcataaatat tgaattgaaa
    15121 ttttgggggg aggtattgta atgacgtttc ttacagtcat gcaatttata gttaacatta
    15181 tcgttgtagg attcatgctt acggttattg ttatcgggct tatttggtta attaaagata
    15241 aaagacaatc acaacatagt gtattaagga attatccttt actagcacgt attagatata
    15301 tttcagaaaa aatgggaccg gaattacgtc agtatttatt ttctggggat aatgaaggga
    15361 aacctttttc acgtaatgat tataaaaata tcgttttggc tggaaaatat aactctcgta
    15421 tgaccagctt cggtactact aaagattatc aagacggctt ttacatacag aacacaatgt
    15481 ttccgatgca acgtaatgag atttcagtag ataatacaac attgttatca acattcattt
    15541 ataaaatcgc gaatgagcgt ttatttagtc gtgaagaata tcgtgtgccg acaaagattg
    15601 atccgtatta cttaagtgat gaccatgcaa taaaattagg tgaacattta aaacatccat
    15661 ttattttaaa acgtatcgta ggacaatctg gtatgagtta tggcgcttta ggaaaaaatg
    15721 ccattacagc tttatctaaa ggtctagcta aagcgggcac ttggatgaat acaggtgaag
    15781 gtggcttatc agaatatcat ttaaaaggta atggggatat cattttccaa attggtcccg
    15841 gtttatttgg tgttcgtgat aaagaaggta attttagtga aggtttattt aaagaggttg
    15901 cacagttatc taacgtacgc gcatttgagc tgaagttggc acaaggtgct aagactcgtg
    15961 gtggtcatat ggaagctgaa aaggtaaacg aagaaattgc taaaatccga aatgttgaac
    16021 cttataaaac aatcaattca cctaaccgtt acgaatttat tcataatgct gaagatttga
    16081 ttcgtttcgt cgatcagttg cagcaattag gtcaaaaacc agtaggattc aaaattgtag
    16141 taagcaaagt ttcagaaatt gaaacacttg tacgtacgat ggtggaacta gataagtatc
    16201 caagctttat tacgattgat ggtggtgaag gtggtactgg tgcaacattc caagaattac
    16261 aagatggtgt tggcttaccg ctatttacag ctctacctat tgtgtctggc atgttagaaa
    16321 aatatggtat tcgagataaa gtgaaattgg cggcatctgg taagttagtg acaccagata
    16381 aaattgcgat tgcactaggt ttaggtgcag attttgtaaa tatcgcacgt gggatgatga
    16441 ttagtgtcgg ttgtataatg agtcaacaat gtcacatgaa tacgtgtcct gtaggtgttg
    16501 caacgacaga tgcgaagaaa gaaaaagcat tgattgttgg agaaaagcaa tatcgtgtca
    16561 caaactatgt aacaagtttg catgaaggct tattcaatat tgcagcagct gttggcgtat
    16621 ccagtcctac agaaattact gctgatcata ttgtatatcg aaaagtcgat ggtgagttac
    16681 aaacgataca tgattataaa ttaaaactca ttagttaact taattatttc gggaaattga
    16741 aagcagcgga ttttagcgtt actgcaaata attttatatt agtagtggat gctggtcaca
    16801 caagaacttc aaatattaaa gccctcagaa tatgaattaa ggtttgtaac cttagtctta
    16861 tctgagggca tttttaagtt ataaactatt tgtcgtccat tttatctttt tcttttaaac
    16921 ctctgtgctt taattgcttt tcaagttttt caaaactaat atctttattt tctttagtcg
    16981 aaacaccaag acgtttattt aattttttca tgtcaacttc tgtgtaatct atgtctaagt
    17041 gttcaattgc ttttttatct ttatagtcta ctttgtattt tacgccttta aggtctttga
    17101 aaatactttc agatttggcg aataactttt tggcttcgtc tttatccata cctagatcgt
    17161 catatttaat tgtgttgatt gtagactgtt ttaaaacttt atcatcttta tatgtgatag
    17221 aagttagtac atgtttacca ctaacatcac cttcatatgt tttggtttgt tctttaccac
    17281 aagctgataa tgcaatgata caaactaatg ctactacaat taatgaacat aattttttca
    17341 aagtcagtcg ccttctttcg atatttgtat tataaagaaa ttataacatt tactaaaaaa
    17401 tgatgttatt caaaaattta aattttgtca tttttttgaa gatatgagtt tttttaagcg
    17461 gattcctcac aaaattttaa aaatatttaa gcctgaaaat gataaagcgg tagggaacgt
    17521 agtgtgttcc actgctgatt tctttttgag cattataggc ggtatgttgg aagaaaagaa
    17581 caaaagtttg tgaacgatag catataggta ttaagaatac gatgaactcg ttggtaatat
    17641 tgacgtactc aagtgcgtac gctataatta gcgtaacaga aaacaaagga gggtttatat
    17701 gattatcact agccctacag aagcgagaaa agatttttat caattactaa aaaatgttaa
    17761 taataatcac gaaccaattt atattagtgg caataatgcc gaaaataatg ctgtgattat
    17821 aggtttagaa gattggaaaa gtatacaaga gacaatatat cttgaatcta ctggtacaat
    17881 ggacaaagta agagaaagag aaaaagataa tagtggtaca acaaatatag atgatattga
    17941 ttgggataat ctttaatgag caattacacg gttaagatta aaaattcagc gaaatcagat
    18001 ttaaagaaaa taaaacattc ttatttaaag aagtcatttt tagaaattgt tgagacttta
    18061 aaaaatgatc cgtataaaat aacacaatct tttgaaaaat tagagcctaa atatttagag
    18121 cgatattcaa gaagaattaa ccatcagcac agggtcgtct ataccgtaga tgatcgaaat
    18181 aaagaagtat taatactatc ggcatggtca cattatgatt aatgaatatt caatatctga
    18241 ataactttaa tgataagtta attaaagaaa ctagtattta agtgtaggga aaatagcgac
    18301 gttaatgcgt tgttatctct acacttttta attttataat agcgcaagac taaacagatt
    18361 gaaattagta acaataaaa
//
LOCUS       DAXWFK010000039        18350 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.039, whole genome shotgun sequence.
ACCESSION   DAXWFK010000039 DAXWFK010000000
VERSION     DAXWFK010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 18350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18350
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.039"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            34..432
                     /locus_tag="ACGEXS_001049"
     CDS             34..432
                     /locus_tag="ACGEXS_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFF8012068.1"
                     /translation="MEKSIKIMTIIGIVVQGLATVFSLLLMVLAASGVMTTDVSTTVN
                     GEVDPVDAETAAAIFTVLFLSLFIFGIISIILGAIGMFKASKNKKMSGILLIIGAVIS
                     GNIITFALWLISGIKLLTNNKPKDEISDLS"
     gene            complement(648..1472)
                     /locus_tag="ACGEXS_001050"
     CDS             complement(648..1472)
                     /locus_tag="ACGEXS_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFF8012069.1"
                     /translation="MKEKHIKWDKIFYGDDWVNNVVETIRTKDILQSVYLKRYLLRAM
                     MAGFIIGIITVFVLSVKATHEPDLPPGIVNMASAITFSFALVLILFTNSELLTSNFMY
                     FTVGLYYKVIKPTRVLKIFLLCFAGNILGAAILFSFMRFSNVMTPDMLNQLSAVIEHK
                     TLSTGFVSILMKAIFANFFINISLVIAMQIDDVLAKMFVMMFGVTIFAFMGYEHVVYN
                     SCLFMGGLIYQVDTLHFIPAISNIAAAFIGNYIGGGLIIGLFYAYLNDHHQFYKNN"
     gene            complement(1709..3016)
                     /gene="brnQ"
                     /locus_tag="ACGEXS_001051"
     CDS             complement(1709..3016)
                     /gene="brnQ"
                     /locus_tag="ACGEXS_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFF8012070.1"
                     /translation="MKKQVIISGLMLFSLFFGAGNLIFPPMLGHTAGQNMWIGMLGFA
                     LTGILLPFITVIVVAFYDEGVESVGNRIHPWFGFIFAVVIYMSIGAFYGIPRAANVAY
                     EIGTRHILPVHNQWTLIIFAAIFFAIVYWISLNPSKIVDNLGKLLTPLLLLMVALLSI
                     AVIFNPESALSAPKDKYITHPFISGSLEGYFTMDLVAALAFSVVIVNGYKFKGLTDRM
                     KILKYVCFSGLIAAILLGMIYFALAYVGASTAPGNFKDGTDILTYNSLRLFGSFGNLV
                     FGMTVILACLTTCIGLVNACATFTKKHVPKFSYKIFALIFSIIGFLFTTLGLEMILKI
                     AVPLLTLIYPVSIALVLISFANMFSTFRFSWAYRLATVITLIISILQILNSFNLLHGV
                     ILKSFMMLPLADIDLAWLVPFMLFAIIGFIIDVFIRRPKQATT"
     gene            3600..4490
                     /locus_tag="ACGEXS_001052"
     CDS             3600..4490
                     /locus_tag="ACGEXS_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HFF8012071.1"
                     /translation="MNKISKYIAIASLSVAVTVSAPQTTNSTAFAKSSAEVQQTQQAS
                     IPASQKANLGNQNIMAVAWYQNSAEAKALYLQGYNSAKTQLDKEIKKNKGKHKLAIAL
                     DLDETVLDNSPYQGYASIHNKPFPEGWHEWVQAAKAKPVYGAKEFLKYADKKGVDIYY
                     ISDRDKEKDLKATQKNLKQQGIPQAKKSHILLKGKDDKSKESRRQMVQKDHKLVMLFG
                     DNLLDFTDPKEATAESREALIEKHKDDFGKKYIIFPNPMYGSWEATIYNNNYKASDKA
                     KDKLRKNAIKQFDPKTGEVK"
     gene            4739..5788
                     /locus_tag="ACGEXS_001053"
     CDS             4739..5788
                     /locus_tag="ACGEXS_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFF8012072.1"
                     /translation="MFLAWNEIRRNKLKFGLIIGVLTMISYLLFLLSGLANGLINMNK
                     EGIDKWQADAIVLNKDANQTVQQSVFNKKDIENKYKKQATLKQTGEIVSNGHQKDNVL
                     VFGVEKSSFLVPSLIEGHKATKDNEVLADETLKNKGFKIGDTLSLSQSDEKLHIVGFT
                     ESAKYNASSVIFTNDATIAKINPRLTGDKINAVVVRDTNWKDKKLNQELEAVSINDFI
                     ENLPGYKPQNLTLNFMISFLFVISATVIGIFLYVMTLQKTSLFGILKAQGFTNGYLAN
                     VVISQTVILALFGTAFGLLLTGVTGAFLPDAVPVKFDVLTLLVFAIVLMIVSVLGSLF
                     SILTIRKIDPLKAIG"
     gene            5801..6478
                     /locus_tag="ACGEXS_001054"
     CDS             5801..6478
                     /locus_tag="ACGEXS_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8012073.1"
                     /translation="MLKFENVTKSFKDGNRNIEAVKDTNFEINKGDIIALVGPSGSGK
                     STFLTMAGALQTPTSGHILINNQDITTMKQKALAKVRMSEIGFILQATNLVPFLTVKQ
                     QFTLLKKKNKNVMSNEDYQQLMSQLGLTSLLNKLPSEISGGQKQRVAIAKALYTNPSI
                     ILADEPTAALDTENAIEVIKILRDQAKQRKKACIIVTHDERLKAYCDRSYHMKDGVLN
                     LENETVE"
     gene            complement(6637..6777)
                     /locus_tag="ACGEXS_001055"
     CDS             complement(6637..6777)
                     /locus_tag="ACGEXS_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012074.1"
                     /translation="MARIPLGALYVNNVKITKTKNKNKVPINKSITIDPHYDNFIFNP
                     HI"
     gene            6728..7717
                     /locus_tag="ACGEXS_001056"
     CDS             6728..7717
                     /locus_tag="ACGEXS_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFF8012075.1"
                     /translation="MIFTLFTYKAPNGMRAMGALANAAIATFLVEAFNKYVGGEVFGI
                     KFLEELGDAAGGLGGVAAAGLTALAIGVSPVYALVIAAACGGMDLLPGFFAGYMIGYV
                     MKYTEKYVPDGVDLIGSIVILAPLARLIAVLLTPVVNSTLIRIGDIIQSSTNTNPIIM
                     GIILGGIITVVGTAPLSSMALTALLGLTGVPMAIGAMAAFSSAFMNGTLFHRLKLGDR
                     KSTIAVSIEPLSQADIVSANPIPIYITNFFGGAIAGLIIAMSGLINDATGTATPIAGF
                     LVMFGFNHPTTIVIYGVVMAIVGALAGYLGSIVFKKYPIVTKQDMINRGAVDA"
     gene            8060..9178
                     /locus_tag="ACGEXS_001057"
     CDS             8060..9178
                     /locus_tag="ACGEXS_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HFF8012076.1"
                     /translation="MSDSEKEILKRIKDNPFISQRELAEAIGLSRPSVANIISGLIQK
                     EYVMGKAYVLNEDYPIVCIGAANVDRKFYVHKNLVAETSNPVTSTRSIGGVARNIAEN
                     LGRLGETVAFLSASGQDSEWEMIKRLSTPFMNLDHVQQFENASTGSYTALISKEGDMT
                     YGLADMEVFDYITPEFLIKRSHLLKKAKCIIVDLNLGKEALNFLCAYTTKHQIKLVIT
                     TVSSPKMKNMPDSLHAIDWIITNKDETETYLNLKIESTDDLKIAAKRWNDLGVKNVIV
                     TNGVKELIYRSGEEEIIKSVMPSNSVKDVTGAGDSFCAAVVYSWLNGMSTEDILIAGM
                     VNAKKTIETKYTVRQNLDQQQLYHDMEDYKNGKFTKVY"
     gene            9153..10076
                     /locus_tag="ACGEXS_001058"
     CDS             9153..10076
                     /locus_tag="ACGEXS_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFF8012077.1"
                     /translation="MANLQKYIEYSREVQQARENNQPIVALESTIISHGMPYPQNVEM
                     ATTVEQIIRNNGAIPATIAIIDGKIKIGLESEDLEILATSKDVAKVSRRDLAEVIAMK
                     CVGATTVATTMICAAMAGIQFFVTGGIGGVHKGAEHTMDISADLEELSKTNVTVICAG
                     AKSILDLPKTMEYLETKGVPVIGYQTNELPAFFTRESGVKLTSSVETPERLADIHLTK
                     QQLNLEGGIVVANPIPYEHALSKAYIEAIINEAVVEAENQGIKGKDATPFLLGKIVEK
                     TNGKSLAANIKLVENNAALGAKIAVAVNKLL"
     gene            10087..11307
                     /locus_tag="ACGEXS_001059"
     CDS             10087..11307
                     /locus_tag="ACGEXS_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFF8012078.1"
                     /translation="MNILFAITGIAFALFVAFLFSFDRKKIDFKKTLIMIFIQVLIVL
                     FMMNTTIGLTILTALGSFFEGLINISKAGINFVFGDIQNKNGFTFFLNVLLPLVFISV
                     LIGIFNYIKVLPFIIKYVGIAINKITRMGRLESYFAISTAMFGQPEVYLTIKDIIPRL
                     SRAKLYTIATSGMSAVSMAMLGSYMQMIEPKFVVTAVMLNIFSALIIASVINPYKSDD
                     TDVEIDNLTKSTETKTLNGKTGKPKKVAFFQMIGDSAMDGFKIAVVVAVMLLAFISLM
                     EAINIMFGSVGLNFKQLIGYVFAPIAFLMGIPWSEAVPAGSLMATKLITNEFVAMLDF
                     KNVLGDVSARTQGIISVYLVSFANFGTVGIIVGSIKGISDKQGEKVASFTMRLLLGST
                     LASIISGSIIGLVL"
     gene            complement(11412..12944)
                     /locus_tag="ACGEXS_001060"
     CDS             complement(11412..12944)
                     /locus_tag="ACGEXS_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HFF8012079.1"
                     /translation="MKEVGFGTLNWVAVIIYLLAMLFIGVYFTKRASQSTNSFFTASG
                     RLPSWVVGFSIYATTLSAITFMSTPEKAFLTDWSYIAGNIAIVAIIPLLIYFYVPFFK
                     KLKVTSAYEYLEARFGPSIRVIGSLLFVVYHLGRVAIVIYLPTLAITSVSDMNPYIVA
                     SLVGLLCILYTFLGGFEGVVWSDFIQGVILLGGALVIIILGVVNIKGGFGTVFADAIE
                     HKKLISADNWKLNTAAAAIPIIFLGNIFNNLYQYTASQDVVQRYQASDSLKETNKSLW
                     TNGILALISAPLFYGMGTMLYSFYTHEAVLPKGFNTSSVVPYFILTEMPPFVAGLLIA
                     AIFAAAQSTISSSLNSISACISIDIKQRFFGKGSERHEVNFARFIIIIAGIFGFGMSL
                     YLIASNSNDLWDLFLFVTGLFGVPLAGVFAVGIFTKRTNTFGVICGLILGIIFAYVYN
                     GVGKGNSPFYVSTISFTVAFVFAYILSFIVPSKHKKDITGLTIFEKDKPSTYISKTAT
                     KK"
     gene            complement(12984..13865)
                     /locus_tag="ACGEXS_001061"
     CDS             complement(12984..13865)
                     /locus_tag="ACGEXS_001061"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFF8012080.1"
                     /translation="MNKDLKGLYAALLVPFDENGQVNEQGLKQIAQNAIETEELDGLY
                     VNGSSGENFLLNTEQKKQVFKVAKEAVGDKVKLIAQVGSLDLNEAIELGKYATELGYD
                     ALSAVTPFYYPFTFEEIRDYYFDIIEATQNNMIIYAIPDLTGVNISIEQFSELFNHEK
                     IVGVKYTAPNFFLLERIRKAFPDKLILSGFDEMLVQATISGVDGAIGSTYNVNGRRAR
                     KIFDLARQGQIQEAYQLQHDSNDIIETVLSMGIYPTLKEILRHRGIDAGLPKRPFKPF
                     NEAHRQTLDQLIAKYDL"
     gene            14023..14883
                     /locus_tag="ACGEXS_001062"
     CDS             14023..14883
                     /locus_tag="ACGEXS_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFF8012081.1"
                     /translation="MYYIAIDIGGTQIKSAVIDKQLNMFDYQQISTPDNKSELITDKV
                     YEIVTGYMKQYQLIQPVIGISSAGVVDEQKGEIVYAGPTIPNYKGTNFKRLLKSLSPY
                     VKVKNDVNAALLGELKLHQYQAERIFCMTLGTGIGGAYKNNQGHIDNGELHKANEVGY
                     LLYRPTENTTFEQRAATSALKKRMIAGGFTRSTHVPVLFEAAEEGDDIAKQILNEWAE
                     DVAEGIAQIQVMYDPGLILIGGGISEQGDNLIKYIEPKVAHYLPKDYVYAPIQTTKSK
                     NDAALYGCLQ"
     gene            complement(15161..15961)
                     /locus_tag="ACGEXS_001063"
     CDS             complement(15161..15961)
                     /locus_tag="ACGEXS_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFF8012082.1"
                     /translation="MKFENRVQRYQHLFTKTDKRIVNYIRQNGYSDAFSTINSLAHAI
                     GTSPATMTRFSHKLDYENFQDLKFNIQQEMTETVIENSPIIQRIHKYHQQIIQQTGEF
                     IDNDIIQTFIDKLQSSRHILFAGLGSSGLSATEFYYRMIRMGLKGNVTTDSHLMKISA
                     SLLSHSDMFIAMSNSGNTSELISAAEVAKSHGAYVVAITNFEGSKLTDCADLVLLTTD
                     QSRNTDHQFINTQIATLFLIDIVSYHLLENTNLSQTYQHTKSIILDNK"
     gene            16101..16769
                     /locus_tag="ACGEXS_001064"
     CDS             16101..16769
                     /locus_tag="ACGEXS_001064"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFF8012083.1"
                     /translation="MLPHGLIVSCQALPDEPLHSSFIMSKMALAAYEGGAVGIRANTK
                     EDILAIKETVDLPVIGIVKRDYDHSDVFITATSKEVDELIESQCEVIALDATLQQRPK
                     ETLDELVSYIRTHAPNVEIMADIATVEEAKNAARLGFDYIGTTLHGYTSYTQGQLLYQ
                     NDFQFLKDVLQSVDAKVIAEGNVITPDMYKRVMDLGVHCSVVGGAITRPKEITKRFVQ
                     IMED"
     gene            complement(16896..18209)
                     /locus_tag="ACGEXS_001065"
     CDS             complement(16896..18209)
                     /locus_tag="ACGEXS_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HFF8012084.1"
                     /translation="MNQYHSNAQQPSAWRFFVYSLVGILCFFIPFTINGNNTIFVDHV
                     HLAIRSIIGPLMPYVALIMILIGTALPIVRRTFMTSITNLVITLFKVAGAMIGIMYVF
                     KIGPSILFKANYGPFLFEKLMMPLSILIPVGAIALSLLVGYGLLEFVGVYMEPIMRPI
                     FKTPGKSAVDAVASFVGSYSLGLLITNRVYKQGMYNKREATIIATGFSTVSATFMIIV
                     AKTLGLMPHWNLYFWITLVITFVVTAITAWLPPISNESTEYYNGQEGEQEVAIEGSRL
                     KTAYAEAMKQNALTPSLVKNVWDNLKDGLEMTVGILPSILSIGFLGLIVANYTPFIDW
                     LGYIFYPFIYIFPIADQALLAKASAISIVEMFLPSLLVTKAAMSTKFVVGVVSVSAII
                     FFSALVPCILATEIKIPVWKLIIIWFLRVALSLLITIPVALLIFG"
BASE COUNT         6418 a         2840 c         3415 g         5677 t
ORIGIN      
        1 gtttgtaaat aataataata aaggagtaag acaatggaaa aatcgatcaa aataatgaca
       61 ataataggaa ttgttgttca gggtttagca acggtattta gtttactatt gatggtttta
      121 gcagcatcag gtgtaatgac tacagatgtg tcaacaacag ttaatggtga ggttgaccca
      181 gttgatgcag aaacagcagc agcaattttc actgtattat tcttatccct attcatattt
      241 ggaatcattt caattatttt aggtgcaatc ggtatgttta aagcatctaa aaacaaaaaa
      301 atgagtggta tattgttgat tattggagct gtaataagtg gtaacataat tacatttgct
      361 ttatggttaa tcagtggtat taaactactt actaataaca agcctaaaga tgaaataagc
      421 gacttatcat aaacatcgta tattgaaatt tcaagatttc ttaagtaatt aaataaagtg
      481 ctctcttaga agtagatttt cagtcaataa ctgcttttaa gagagcattt tgtatttgat
      541 aatgctcata gcatcggata aaaatcattg acgaacttta aaaaaatacg ttagacaatg
      601 cacttctaaa tattaaaggc atcgactaac gcattgttca aatatagtta attattttta
      661 taaaattgat gatgatcatt caagtaagca tagaataaac ctataatgag tccgcctcca
      721 atatagttac cgataaaagc cgcagcgata ttcgaaatag ctggtatgaa gtgtaatgta
      781 tcaacttgat aaattaaacc acccataaat aagcaactgt tgtaaacgac gtgttcataa
      841 cccataaagg cgaatatggt aacaccgaac atcatgacaa acatttttgc gagtacatcg
      901 tcaatttgca tggcaataac taatgaaata ttgataaaga aattggcgaa gatcgctttc
      961 attaatatac ttacaaaacc agtagacaac gttttatgct ctataactgc tgataactga
     1021 tttaacatat ctggcgtcat tacatttgaa aaacgcatga aactaaataa aatagcagca
     1081 cctaaaatat ttcctgcaaa gcataataaa aatattttca atactctagt tggtttaatt
     1141 actttataat acaggcctac agtaaagtac atgaagttac tggttagtag ttcggagttt
     1201 gtaaataaaa tgagtactaa cgcaaagctg aatgtaatgg cactggccat attcacaatg
     1261 cctggcggta aatctggttc gtgtgttgct ttaactgata atacgaagac cgtaataatc
     1321 ccgataataa atcctgccat catcgcgcgt aataaataac gttttaaata aacgctttgt
     1381 aatatatctt ttgttcttat cgtttcgact acgttattta cccagtcgtc cccataaaaa
     1441 atcttatccc atttaatatg tttctccttc acaatgacat cctcactttt catttgttaa
     1501 tcagagtata ctatgaattg ccttatttgt gaataatttc acaaaattaa ttttaaaaac
     1561 taatattttt gaataacagc taggattttt taacaaatca tttattttaa aataatcaat
     1621 agacagacgg tatcactgaa aagtcatgaa tttcatataa aaaagcctag taacgatggg
     1681 tcaatcatta ctaggcagtg agcatttatt aagttgtcgc ttgtttcgga cggcgtataa
     1741 atacatcgat tatgaaaccg ataatagcaa agagcatgaa tggtacaagc caagctaaat
     1801 cgatatctgc taaaggtaac atcataaacg atttcaaaat aacaccgtgt aataagttga
     1861 aactatttag tatttgtaaa attgaaataa tcaatgtaat aacagttgcg agtcgatagg
     1921 cccaactgaa tctgaatgtg ctaaacatgt tagcaaatga tatgagtaca agtgcaatcg
     1981 acacgggata tattaaagtc aataatggga cagcaatttt taaaatcatt tctaaaccaa
     2041 gtgttgtaaa taagaaccct atgatagaga aaataagtgc gaatatttta taagaaaact
     2101 taggtacgtg tttcttagta aatgtggcgc aagcattgac gagtcctata catgttgtta
     2161 ggcatgcaag gataaccgtc attccaaata cgaggttacc gaacgaacca aataatcgta
     2221 atgagttgta cgtcaatata tctgtaccat ctttaaagtt tcctggagct gttgatgccc
     2281 caacgtatgc aagtgcaaag taaatcattc caagtaatat ggctgcaata agacctgaaa
     2341 agcagacata ttttaaaatt ttcatgcgat ctgtgaggcc tttaaactta tagccattga
     2401 caatgactac ggaaaaagct aacgcagcaa caagatccat tgtaaaatag ccttccaaac
     2461 ttcctgaaat gaaaggatgt gttatatatt tatccttagg tgcacttagt gcagattcag
     2521 ggttgaaaat gacagcaata cttaatagag cgaccattaa tagtaataac ggtgttaata
     2581 atttacctaa attatcaacg attttcgatg gatttaaact aatccagtaa acgatggcaa
     2641 aaaagattgc tgcgaatata attaaagtcc attggttatg cacaggtaaa atgtgtcttg
     2701 taccaatttc gtacgcgaca tttgcagcac gtggaatacc gtaaaatgct ccgatagaca
     2761 tgtaaatcac gacagcaaaa ataaacccga accatggatg tatacgattg cctacacttt
     2821 caacaccttc atcataaaat gcaacaacaa taacagtaat aaaggggagt aatatgcctg
     2881 taagggcaaa gcctagcata ccaatccaca tattttgacc cgctgtatgg ccaagcatgg
     2941 gcgggaatat taaatttccg gctccaaaaa atagtgaaaa taacatgagg cccgaaataa
     3001 taacttgttt tttcaatgta atttccttcc taaaactaga tattcataat aatttaaaaa
     3061 atctgaaaaa taaaaacgtt cttactttat ctttaaatga caatactatg attctattat
     3121 tttataaaaa agattgcaat aataacgtct tgctattatt ggataatgac aattaaagtg
     3181 tcgtttttat gaattaaaat aggtgaaagt aagttggaaa tacgttggag cgttgtgatt
     3241 tttatgagaa attgtatgag tgaggactgc tatttataaa aggtaaacta tggatgagtt
     3301 tatggttgtt ccaataggta aataagagat agcacactgt acacataatg atacgtggat
     3361 gagtgaatat gtctgatagg aaggcctagt cactcaaatg aatttaagtt tattacgtga
     3421 taaatcacaa atctctctca tgtgataggt ctcccattaa atcatgctta tatgaaatgt
     3481 tcacatattt gttagctttt caagaaataa tattaaatat cttttcatag tatagcaata
     3541 ttaaataaat gcatttataa ttttaactgt attttaaata ttaatcatga ggtgataaga
     3601 tgaataaaat ttcaaagtat attgcaatag catcattatc ggtagcggtt acagtttcag
     3661 caccacaaac gacaaattct acagcgtttg ccaaaagttc tgctgaagtt caacaaacgc
     3721 aacaagcttc tataccagca tcacaaaagg cgaatcttgg taatcaaaat attatggcag
     3781 tggcttggta tcaaaattca gctgaagcaa aagcattata tttacaaggt tataacagtg
     3841 caaagacaca gttagataaa gagattaaaa agaataaagg taaacataag ttagctattg
     3901 ctttggattt agatgaaaca gttttagata attctccata tcaaggctat gcatcaatac
     3961 ataataaacc tttcccagaa ggttggcatg aatgggtaca agctgctaaa gctaaacctg
     4021 tctatggcgc aaaagaattc ttgaaatatg ctgacaaaaa aggtgtcgat atctactata
     4081 tttctgatag agataaagaa aaagatttaa aggcaacaca aaagaactta aaacaacaag
     4141 gtatccctca agctaagaag agtcatattt tactaaaagg taaagatgat aagagtaaag
     4201 aatcacgcag acaaatggtt caaaaggatc ataaacttgt catgctattt ggagataatt
     4261 tattagactt tacagatcca aaagaagcta cagctgaatc tcgtgaagca ttaattgaaa
     4321 aacataaaga cgatttcggt aagaaatata tcattttccc taacccaatg tatggtagtt
     4381 gggaagctac gatttacaac aataactata aagcaagtga caaagcaaaa gataaattac
     4441 gtaaaaatgc tattaagcaa ttcgatccta aaacaggcga agttaaataa tatatgaatt
     4501 ggacgtctac atgtacttaa aggtatatgt aggcgttttt aaattgatcc ttaattttaa
     4561 atgtaccgta caaaaataat agacaattaa ttgcattttc cataaatttg cttttaatat
     4621 aaaaagttcg ggtataatga aggataagtg tatgtataac attacagagc gcaacacgac
     4681 gtacgtgcat ctaagaatat aaacaatgtt taaatcatag tagtagggag agaaatgcat
     4741 gtttttagct tggaatgaaa tacggcgcaa caaattgaag tttggactaa ttattggtgt
     4801 gttaacgatg attagttact tgctattttt attatctgga ttggcgaatg gtcttatcaa
     4861 tatgaataaa gaaggcattg ataagtggca agcagatgcc attgttctaa ataaagatgc
     4921 caatcaaact gtgcaacaat ctgtttttaa caagaaagat attgaaaata aatacaagaa
     4981 gcaagctact ttgaagcaaa caggggaaat tgtgtctaat ggccatcaaa aagacaatgt
     5041 tttagtgttc ggtgttgaaa agtcatcatt tttagttccg agtttaatag aagggcataa
     5101 agcgactaaa gataatgaag tgttagctga tgaaacgctt aaaaataaag gatttaaaat
     5161 tggtgacaca ttatcactat ctcaatcaga tgaaaaattg catatcgtag gttttacaga
     5221 aagtgcaaaa tataatgcgt catcagtcat tttcacgaat gacgctacca ttgccaagat
     5281 caatcctaga ttgactggag ataaaattaa tgcagttgtt gtacgtgata caaattggaa
     5341 agacaaaaaa ttaaaccaag agcttgaagc ggtaagtatt aatgacttta ttgaaaattt
     5401 accaggttat aaaccacaga acttaacatt aaactttatg atttcattct tatttgtcat
     5461 ttcagctaca gttataggca ttttcctata tgtcatgaca ttacaaaaga cgagtttatt
     5521 tggcatatta aaagctcaag gatttacgaa tggctatttg gcgaatgtgg taatttcgca
     5581 gacggtcata ttagcactat ttggtacggc atttggctta ctgttaacag gcgttacagg
     5641 tgcattttta cctgatgcag tacctgtcaa attcgatgta ctaacattgc tcgtatttgc
     5701 aattgtgtta atgattgtct ctgtattagg aagtttattc tccattttaa caattagaaa
     5761 aatagatccg ttaaaggcga ttgggtagga ggtgtagcaa atgttgaaat ttgaaaatgt
     5821 aacaaagtca tttaaagatg ggaatcgtaa cattgaagcg gttaaagata caaattttga
     5881 gataaataaa ggtgatatta tagcattggt tggaccttct ggctctggta aaagtacatt
     5941 tctaactatg gcaggtgctt tacaaacacc gacatctggg cacattttaa tcaataacca
     6001 agatattacg acaatgaagc aaaaagcatt ggcaaaagtt agaatgtctg aaataggttt
     6061 tattttacaa gctacaaacc ttgtaccatt tttaacggta aagcaacaat ttacattatt
     6121 gaaaaagaaa aataagaatg ttatgtctaa tgaagactat cagcaactta tgtcacaatt
     6181 aggtctaact tcattgctta ataagttacc ttcagaaatt tcaggtggtc agaaacaacg
     6241 tgtggcgata gccaaagcgt tatatacgaa tccgtcgatt attttagcgg atgaacctac
     6301 cgcggcgtta gatactgaaa atgcgattga agtcattaaa attctacgtg atcaagccaa
     6361 acaaagaaag aaagcatgta ttattgttac acatgatgaa cgacttaaag catattgtga
     6421 tcgttcatat catatgaaag atggcgtcct taatcttgaa aatgaaacag tagaatagtt
     6481 ttattaagcc ggtacatcat gtgccggtat ttttatgttt atgtattatt tgaataaact
     6541 ttcacattca attaataata attattatcg aaaatcagaa atattccgtg aaatataata
     6601 ttttttgtag taaaatggcc tctaagtatt caatatttaa atatggggat tgaatataaa
     6661 attatcgtaa tgggggtcaa tggttatgga tttattgata ggtactttat ttttattttt
     6721 ggtcttagtg atttttacat tatttacata taaagcgcct aatggtatgc gtgccatggg
     6781 agcattagct aatgcagcaa tcgcaacatt tttagtggaa gcatttaata aatatgttgg
     6841 tggcgaagta ttcggtatta aatttttaga agagctagga gacgctgcgg gaggtctagg
     6901 tggtgtcgct gccgctggat taacagcatt agctatcggt gtgtcaccag tatatgcatt
     6961 agttatagca gccgcgtgcg gtggtatgga tttattacca ggtttctttg cgggttatat
     7021 gattggatat gtgatgaaat atacagagaa atatgtgccg gatggtgtcg acttaattgg
     7081 atcgattgtc atcttagcgc cattagctcg tcttattgca gtattattaa cgccagtagt
     7141 gaatagtaca ttgattcgaa ttggtgatat tatccaaagt agtacgaata cgaatccaat
     7201 tatcatgggt atcattttag gtggtattat tacggttgtc ggcacagcgc cattgagttc
     7261 aatggcattg acagcattat taggtttaac gggtgtacct atggctattg gtgccatggc
     7321 agcatttagt tcggcattta tgaatgggac gctattccat cgcttaaaat taggtgatcg
     7381 taagtctacg attgcagtaa gtattgaacc tttatcacaa gcagatattg tatcagccaa
     7441 tccaattcca atctatatta caaatttctt tggtggtgcg attgctggtt taattattgc
     7501 tatgtcaggt ttaattaacg atgcgacagg tacagctaca ccgattgcag gatttttagt
     7561 tatgtttgga tttaatcatc cgacgacaat tgtgatttat ggtgtagtaa tggcgattgt
     7621 aggtgcgctt gcaggttatc ttggttcaat tgtatttaaa aaatatccaa ttgttactaa
     7681 gcaagacatg attaatcgag gtgcagtaga cgcatagcat catcatattg aatagtaaaa
     7741 acaaataaaa catagtaacg tgattcagtc gatgtaacag tcgataatga gtcacgtttt
     7801 tttatagaaa aatacaagac ataaaaatgt cataatttat tgtcgacaaa tatcatactg
     7861 tataaacatt tatcattttc tcaagtacct tttacacgat ggaatgaact tactttttac
     7921 gaaattatgc gtattttata aacaaatatc attgatataa cggtaaatgt aagcgtttac
     7981 aacagaaata acagcatgct acgatatttt tgtaaattca ctgattcaag tattttaagt
     8041 caatatgagg agggatgtta tgagcgattc tgagaaagaa attttaaaaa gaattaaaga
     8101 taatccgttt atttcacaac gtgaacttgc tgaggcaatt ggattatcta gacccagcgt
     8161 agcaaacatt atttcaggat taatacaaaa ggaatatgtt atgggaaagg catatgtttt
     8221 aaatgaagat tatcctattg tttgtattgg cgcagcgaat gtagatcgta agttttatgt
     8281 gcataaaaat ttagttgcag aaacatcaaa tcctgtaacg tcaacacgct ctattggtgg
     8341 cgtagcaaga aatattgctg agaacttagg taggcttggc gaaacggtcg cttttttatc
     8401 tgctagtgga caagatagtg aatgggaaat gattaaacga ttgtccacac catttatgaa
     8461 tttggatcat gttcaacaat ttgaaaatgc gagtacaggt tcatatacag ctttaattag
     8521 taaagaaggc gacatgacat atggcttagc agatatggaa gtgtttgact acattacgcc
     8581 tgaattttta attaagcgtt cacacttatt gaaaaaggct aagtgcatta ttgtagattt
     8641 gaatttaggc aaagaggcat taaacttctt atgtgcctat accacgaaac atcaaatcaa
     8701 attagttatc accacggttt cttccccaaa aatgaaaaat atgcctgatt cattacatgc
     8761 tattgattgg attatcacga ataaagatga aacagaaaca tacttaaatt taaaaataga
     8821 atctactgat gatttaaaaa tagctgctaa acgctggaat gatttaggtg ttaaaaatgt
     8881 tattgtgaca aatggcgtga aagaactcat ttatcgaagt ggtgaggaag aaatcattaa
     8941 gtcagttatg ccatcaaata gtgtgaaaga tgttacaggt gcaggcgatt cattctgtgc
     9001 tgcagtagtg tatagctggt taaatgggat gtctactgaa gatatattaa ttgctggtat
     9061 ggttaacgca aagaaaacga tagaaacgaa atatacagtt aggcaaaacc tagatcaaca
     9121 gcaactttat cacgatatgg aggattataa aaatggcaaa tttacaaaag tatattgagt
     9181 attctcgaga agttcagcaa gcacgggaga acaatcaacc gattgtagca ttagaatcaa
     9241 caattatttc gcatggtatg ccgtacccac aaaatgttga aatggcaaca acagtagagc
     9301 aaattatcag gaataatggt gccattccag caaccatagc cattatagat ggcaaaatta
     9361 aaattggttt agaaagcgaa gatttagaaa tactggcaac tagtaaagac gttgctaaag
     9421 tatctagaag ggatttagca gaagttattg cgatgaagtg tgttggtgct actactgtag
     9481 cgacgacgat gatatgtgct gcaatggctg gtattcaatt ttttgttaca ggaggtattg
     9541 ggggcgtcca taaaggtgca gaacatacga tggacatttc agcagactta gaagaactgt
     9601 ctaaaacaaa tgtcactgtt atctgtgcag gtgccaaatc aattttagac ttacctaaga
     9661 cgatggagta tttagaaaca aaaggcgttc cagttattgg atatcaaacg aatgaattgc
     9721 cagcattctt cactcgcgaa agcggtgtta agttaacaag ttcggttgaa acgccagaac
     9781 gacttgctga cattcattta acaaaacagc agttaaatct tgaaggtggc attgttgttg
     9841 ctaatccaat tccatatgag catgccttat caaaagcata tattgaggca atcataaatg
     9901 aagctgttgt tgaagcggaa aatcaaggta ttaaaggtaa ggacgccaca ccgttcttgt
     9961 tagggaaaat tgtagaaaaa acgaatggta aaagtttagc agcaaatata aaacttgttg
    10021 aaaacaatgc ggcgttgggt gctaaaattg ctgtcgctgt taataaatta ttgtaggtga
    10081 tgatacatga atattttatt cgctatcaca gggatagcat ttgcactatt tgttgcgttt
    10141 ttattcagtt ttgatcgtaa aaaaatagac ttcaaaaaga cgttaataat gatatttatt
    10201 caagtgttga tcgtgttatt tatgatgaac acaacgattg gtttgacaat tttaactgca
    10261 ctaggttcat tttttgaagg gctaataaat attagtaaag caggcataaa ttttgttttt
    10321 ggagatatac aaaataaaaa tggctttacg ttctttttaa acgtattact gccattagtt
    10381 tttatttctg tattaatagg catctttaat tatattaagg tattaccatt tattatcaaa
    10441 tatgtaggta tcgctattaa taaaataact agaatggggc gcttagaaag ttattttgct
    10501 atttcaacag caatgtttgg gcaaccagaa gtatatttaa caataaaaga tattattcca
    10561 agattatcta gagcgaaatt atatacaatt gcgacgtctg gtatgagtgc tgttagtatg
    10621 gcaatgctag gttcatatat gcagatgatt gaacccaagt tcgtagttac agcagtaatg
    10681 ttaaatattt ttagtgcgct tatcatcgcc agtgtaatca atccctataa atctgatgat
    10741 actgatgttg aaattgataa cttaacgaaa tccacagaaa ctaaaacatt gaatggaaaa
    10801 acaggaaaac ctaagaaagt tgcctttttc caaatgattg gtgatagtgc gatggatggg
    10861 tttaaaatcg ctgttgtagt agccgtaatg ttgttagcat ttatttcatt aatggaagca
    10921 attaatatca tgtttggtag tgttggtttg aactttaaac agcttattgg ctatgtgttt
    10981 gcaccaatcg cattcttaat ggggattcca tggagcgaag ctgttccagc tggctcttta
    11041 atggcgacta aattaattac aaatgagttt gtagcaatgc ttgattttaa aaatgtcctg
    11101 ggtgatgtat cagctcgaac acaaggtatc atttcagttt acttagtaag cttcgctaat
    11161 tttggtacgg ttggtatcat cgtaggttca attaaaggca ttagtgataa acaaggagaa
    11221 aaagttgcat cctttacaat gaggttgcta cttggttcaa ctctagcttc aatcatttca
    11281 ggatcaatca ttggcttagt attgtaaatg aatcgaagta cctaaattaa attcatggca
    11341 aagctaaacc ccgtcaccaa gttggcgcaa cagcgcatca taacttagtg acggggtttt
    11401 atcataacaa tctacttttt cgtagccgtt tttgaaatgt atgttgatgg tttatctttt
    11461 tcaaaaattg ttaatcccgt tatatctttt ttatgttttg aagggacaat gaagctaagt
    11521 atataagcaa agacaaaagc aactgtaaat gaaatggtag atacatagaa aggtgagtta
    11581 cctttgccaa caccattata gacataagca aagatgatac ccaatattaa tccacaaata
    11641 acaccgaatg tattcgtacg tttagtgaaa ataccaactg caaatacacc agccaatgga
    11701 acgccgaata atccagtcac aaacaagaat aaatcccata agtcatttga attagaagca
    11761 attaagtata gtgacattcc aaaaccgaaa atacctgcaa tgataataat gaaacgtgca
    11821 aagttaactt cgtgtcgctc gctacctttt ccgaagaagc gttgcttaat gtcgattgaa
    11881 atacaagcag atatagaatt taaactagat gaaatggtag actgtgcagc ggcgaaaatg
    11941 gctgcaataa gtaatcctgc tacaaatggt ggcatctcag tcaaaatgaa atatggcact
    12001 acagatgatg tattgaagcc ttttggtaaa acagcttcat gtgtataaaa tgaatacagc
    12061 attgtaccca taccataaaa taagggtgct gaaattaaag ctaggatacc atttgtccat
    12121 aacgatttat ttgtttcttt taaactatca gaagcttgat aacgctgcac gacgtcttga
    12181 ctcgctgtgt attgatacaa gttgttgaaa atatttccta ggaaaataat tggaatggca
    12241 gctgccgcag tatttagttt ccaattgtct gcactaatta attttttgtg ctcaatcgca
    12301 tctgcaaaga cagtgccgaa accgccttta atgttcacaa cacctagaat aataataact
    12361 aaagcgccgc ctaataaaat gacgccttga atgaaatcac tccaaaccac accttcgaaa
    12421 ccacctaaaa atgtatataa aatacatagt aaaccaacga gtgatgcaac gatataaggg
    12481 ttcatgtctg atacagatgt gattgctaat gttggtaagt agataacaat tgcaacacgc
    12541 cctaaatggt aaacgacaaa taataatgag ccaatgacac gtatgctagg gccaaatcta
    12601 gcttctaaat attcatatgc agatgttacc tttaactttt taaagaaagg gacatagaaa
    12661 taaataagta atggaataat tgcgacgata gcaatgttac cagcgatata tgaccaatct
    12721 gttaaaaatg ctttctctgg tgtcgacata aatgtaatcg cacttaacgt agtagcataa
    12781 attgaaaagc caactaccca agatggcaag cgaccacttg cggtaaagaa actattggta
    12841 ctttggctcg cgcgcttggt aaaataaacg ccaatgaaca acatagctag tagataaatg
    12901 ataacggcaa cccagtttag tgtgccaaat ccaacttctt tcatgggcaa catccccttt
    12961 acaatgtatt gattctttga tgtctataaa tcgtattttg caatgagttg atctaatgtt
    13021 tgtcgatgtg cttcgttaaa aggtttgaaa ggtcttttcg gtaatcctgc atcaatgcca
    13081 cgatgacgta atatttcttt caatgttgga taaatcccca ttgataacac tgtttcgata
    13141 atgtcgtttg aatcatgttg cagttggtaa gcttcttgaa tttgaccttg tcgtgctaag
    13201 tcgaagattt ttcttgcacg gcgaccatta acgttatatg tagaaccaat tgcaccatct
    13261 acgccagaaa tcgtagcttg aactaacatt tcatcaaagc cagataagat taatttgtct
    13321 gggaatgctt ttctaatacg ttcgagtagg aagaagtttg gcgctgtata tttaacacca
    13381 acaatttttt catgattaaa tagctcgctg aattgttcaa tagaaatatt cacacctgtt
    13441 aaatctggta ttgcataaat aatcatattg ttctgagttg cttcgataat atcgaaatag
    13501 taatctctaa tttcttcaaa agtaaatgga tagtagaatg gtgttacggc agaaagtgca
    13561 tcataaccga gttctgtggc atattttcca agttcaatgg cttcatttaa atctaacgaa
    13621 cctacttgag caatcaattt cactttatcc ccaactgcct ctttggcaac cttgaaaact
    13681 tgcttcttct gctctgtatt taataaaaag ttttcgcctg agctaccatt tacataaaga
    13741 ccgtctaatt cttcagtttc aatggcattt tgagcaattt gtttaagtcc ttgttcattt
    13801 acttgaccat tttcatcaaa aggaacgagt aacgctgcat ataaaccttt taaatctttg
    13861 ttcattatga agtccctcca aaaatcattt gataatatag tttacagcta taattgtaaa
    13921 cgctatcata aaatgtaaca atatcttttt gaaaattgta gtcatattta tgtataatta
    13981 atgaaaatgt ttttcaaaat caatagaaat ggagtgagta aggtgtatta catcgcaatc
    14041 gatattggag gcactcaaat taaatcggca gttattgata agcaattgaa tatgtttgac
    14101 tatcaacaaa tatcaacgcc ggacaacaaa agtgagctta ttactgacaa agtatatgag
    14161 attgtaacag gatatatgaa gcaatatcag ttgatccaac ctgtcatagg tatttcatca
    14221 gcaggcgttg ttgatgaaca aaaaggcgaa attgtatacg cagggccaac cattccgaat
    14281 tataaaggta ctaattttaa gcgattatta aaatcactgt ctccttatgt caaagtaaaa
    14341 aatgatgtaa acgctgcatt actaggcgaa ttgaaattac atcaatatca agcagaacgg
    14401 atcttttgta tgacgcttgg tacaggcatt gggggtgcgt acaagaataa tcaaggtcat
    14461 attgataatg gtgagcttca taaggcaaat gaagttgggt atttattgta tcgtccaact
    14521 gaaaatacaa cgtttgagca acgtgctgca acgagtgcat tgaaaaagcg catgattgcc
    14581 ggaggattta cgagaagcac acatgtgcca gtattgtttg aagcagctga agaaggtgat
    14641 gatattgcaa aacaaatatt gaatgagtgg gcagaagatg tagcagaagg gattgcccaa
    14701 atacaggtca tgtatgatcc agggcttata ttaattgggg gcggtatatc tgaacaagga
    14761 gataatctca ttaaatatat cgagccgaaa gttgcacact atttaccaaa agactatgtt
    14821 tatgcaccaa tacaaacgac taagagtaaa aatgatgcag cattatatgg ctgtttgcaa
    14881 tgatagttga aagaaggagt cattctaaaa tagaatttga aaccgttacg agagatgaga
    14941 gctgttgtta gttccacaca tcacactcta tctaggacca atctaaacta tatcaaccaa
    15001 cagtgtgcca cgggcaaatt aaattgaaga agctgagata ttaaaatttt agaaaatgta
    15061 aaaaaatatt tggtattgaa attaaaaaag cacctagcaa ctcgttggga caatcacgat
    15121 gattgtctac agttgcaggt ggatttgaat atactactag ttatttgttg tctaggataa
    15181 tagatttagt atgttgataa gtttgactca gattcgtatt ttctaataaa tgataactca
    15241 cgatatcgat taaaaagagt gtcgcaattt gtgtgttgat aaattgatgg tcggtattac
    15301 gcgattgatc cgttgttaaa agtactaaat ctgcacaatc tgtaagttta ctaccttcaa
    15361 aatttgtgat ggcaacgaca tatgcaccat gagatttggc gacttccgct gcagaaatta
    15421 attccgaagt attaccacta tttgacatag caataaacat atccgaatga gatagtaggg
    15481 atgccgatat tttcattaaa tgtgaatcgg tagtaacatt accttttagc cccatacgaa
    15541 tcatacgata ataaaattca gtcgctgata aaccagagct acctagtcca gcaaagagta
    15601 tatgtcgact tgattgaagt ttgtcgataa aggtttggat aatgtcgtta tcaataaatt
    15661 caccagtttg ttgaatgatt tgttgatgat atttatgaat tctttgaata attgggctat
    15721 tttcaataac tgtctctgtc atttcttgtt gaatattaaa ttttaaatct tggaaattct
    15781 cataatccag cttatgacta aagcgtgtca tcgttgctgg tgatgtacca atcgcatggg
    15841 ctaaggagtt aatcgttgaa aaggcatcgc tataaccatt ttgtcttata taattgacga
    15901 tgcgtttatc agtttttgta aataaatgtt gataacgttg aacacgattc tcaaatttca
    15961 ttgtgtcacc ccttcatctt aatgattact attatatatg aaaaatattt tcaagatagt
    16021 aaaaagcatt gataaaaatt atcttaatga tatattgtaa atgactttac gtgaaaaaac
    16081 gacttatgga gtgaggaata atgttaccac atggattaat agtatcttgt caggcactac
    16141 cagatgaacc attgcattca tcttttatta tgtcgaaaat ggcattagct gcgtatgaag
    16201 gtggtgctgt tggtattcgc gcaaatacta aggaagacat tttagcaatt aaagaaacgg
    16261 tagatttacc agttattggc attgtgaaac gtgactatga tcactcagat gttttcatta
    16321 ctgcaacgtc aaaagaagtt gatgaactga tagaaagcca atgtgaagtc attgcattgg
    16381 atgcaacgtt acagcaacgt ccgaaagaaa cgttagacga attagtatca tatattagaa
    16441 cacatgcacc gaacgttgaa atcatggctg atatcgcgac cgttgaagaa gctaaaaatg
    16501 ccgcacgact tggctttgat tatattggca cgacgttaca tggctatact agttatacgc
    16561 aaggacaatt actttatcaa aatgacttcc aatttttaaa agatgtacta caaagtgttg
    16621 atgcaaaagt tattgcggaa ggtaatgtca ttacaccgga tatgtataaa cgtgtgatgg
    16681 acttaggcgt tcattgttca gtcgttggtg gtgcgataac acgaccaaaa gaaattacga
    16741 aacgttttgt tcaaattatg gaagattaaa tgataacgat aaaaaaacga gatgaccatc
    16801 attaattaaa ggcacctaat tatcttaggt ggctgaatga atgtaatggg ttcatctcgt
    16861 tttgtttgtt tatgatagtg attttatttt caactttatc caaaaataag taaagcgacg
    16921 gggatggtga ttaatagcga caacgccacg cgtaaaaacc aaatgatgat gagtttccag
    16981 acaggtattt taatttcagt tgctagtata catggcacta atgctgagaa aaagataatg
    17041 gctgatacgc ttactacacc gacgacaaat ttagtactca ttgcagcttt agttactaac
    17101 aaagatggta gaaacatctc tacaatagaa atcgctgacg cttttgctag taaagcctga
    17161 tcagcaattg ggaaaatata aataaatgga tagaagatat agccaagcca atcaatgaat
    17221 ggtgtatagt tcgctacaat cagtcctaaa aaaccaatcg ataatataga aggtaaaata
    17281 ccaacagtca tttctaaacc gtctttcaaa ttgtcccaaa cgttcttcac gagagatggt
    17341 gttaatgcat tttgtttcat cgcctctgca tatgcagttt tcagtctgct tccttcaata
    17401 gcaacttctt gttctccttc ttgtccgtta taatattctg ttgattcatt gctgattggc
    17461 ggtagccatg cagtaattgc agtcacgaca aatgtgatga ctaaagttat ccaaaagtat
    17521 aaattccaat gcggcattaa tcctaaagtt ttagcaacga taatcataaa agttgctgaa
    17581 actgttgaaa agccagtcgc aataatcgtg gcttctcgtt tgttgtacat cccttgctta
    17641 tagacacgat tagtaatcaa taatcctaag gaataactgc cgacaaacga agccactgca
    17701 tcgacagcgg attttcctgg tgttttaaaa ataggtctca taataggctc catataaaca
    17761 ccgacaaatt ctaataagcc atagcccact aataaagaaa gcgcaattgc acctactgga
    17821 attaagatac ttaatggcat cattaatttt tcaaacaaaa acggaccata gttagcttta
    17881 aatagtattg atggaccgat tttaaataca tacattatac cgatcattgc acctgcaact
    17941 ttaaataatg taatgaccaa gtttgtgatt gaagtcataa aagtacgtct cactattggt
    18001 aacgctgtac caattaaaat cataatcagt gcaacatagg gcataagtgg acctatgatt
    18061 gagcgaatgg ctagatgaac atgatcgacg aaaatagtgt tgttaccatt aatcgtaaaa
    18121 ggaataaaga aacatagtat gcccactaaa ctatagacaa aaaaacgcca tgcacttggt
    18181 tgttgtgcat tagaatgata ttgattcatt aaagcaaccc ctttgtttaa atgaatacac
    18241 aaaactgtat gatgcatctt ccccttaatg agatgaatca ttattttaat ttagaaaaat
    18301 ctgaaaactt actataattg tatagtttga attattttca taccaataca
//
LOCUS       DAXWFK010000040        17829 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.040, whole genome shotgun sequence.
ACCESSION   DAXWFK010000040 DAXWFK010000000
VERSION     DAXWFK010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17829
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.040"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            164..1354
                     /locus_tag="ACGEXS_001066"
     CDS             164..1354
                     /locus_tag="ACGEXS_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine aminotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HFF8012085.1"
                     /translation="MTKSEKIIELTNHYGAHNYLPLPIVISEAEGVWVKDPEGNKYMD
                     MLSAYSAVNQGHRHPKIIQALKDQADKVTLVSRAFHSDNLGEWYEKICKLAGKDKALP
                     MNTGAEAVETALKAARRWAYDVKGIEPNKAEIIAFNGNFHGRTMAPVSLSSEAEYQRG
                     YGPLLDGFRKVDFGDVDALKAAINENTAAVLVEPIQGEAGINIPPEGYLKAIRELCDE
                     HNVLFIADEIQAGLGRSGKLFATDWDNVKPDVYILGKALGGGVFPISVVLADKEVLDV
                     FTPGSHGSTFGGNPLACAASIAALDVIVDEDLPGRSLELGDYFKEQLKQIDHPSIKEV
                     RGRGLFIGVELNESARPYCEALKEEGLLCKETHDTVIRFAPPLIITKEELDLALEKIR
                     HVFQ"
     gene            1463..2707
                     /locus_tag="ACGEXS_001067"
     CDS             1463..2707
                     /locus_tag="ACGEXS_001067"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HFF8012086.1"
                     /translation="MTENNNLVTSTQGIIKEALHKLGFDEGMYDLIKEPLRMLQVRIP
                     VRMDDGTVKTFTGYRAQHNDAVGPTKGGVRFHPDVDEEEVKALSMWMTLKCGIVNLPY
                     GGGKGGIVCDPRQMSIHEVERLSRGYVRAISQFVGPNKDIPAPDVFTNSQIMAWMMDE
                     YSALDKFNSPGFITGKPIVLGGSHGRDRSTALGVVIAIEQAAKRRNMQIEGAKVVIQG
                     FGNAGSFLAKFLYDLGAKIVGISDAYGALHDPNGLDIDYLLDRRDSFGTVTNLFEETI
                     SNKELFELDCDILVPAAISNQITEDNAHDIKASIVVEAANGPTTPEATRILTERGILL
                     VPDVLASAGGVTVSYFEWVQNNQGYYWSEEEVNEKLREKLEAAFDTIYELSQNRKIDM
                     RLAAYIIGIKRTAEAARYRGWA"
     gene            complement(3096..4025)
                     /locus_tag="ACGEXS_001068"
     CDS             complement(3096..4025)
                     /locus_tag="ACGEXS_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFF8012087.1"
                     /translation="MTNSSKSFTKFMAASAVFTMGFLSVPTAGAEQTNQIANKPQAIQ
                     WHTNLTNERFTTIAHRGASGYAPEHTFQAYDKSHNELKASYIEIDLQRTKDGHLVAMH
                     DETVNRTTNGHGKVEDYTLDELKQLDAGSWFNKKYPKYARASYKNAKVPTLDEILERY
                     GPNANYYIETKSSDVYPGMEEQLLASLKKHHLLNNNKLKNGHVMIQSFSDESLKKIHR
                     QNKHVPLVKLVDKGELQQFNDQRLKEIRSYAIGLGPDYTDLTEQNTHHLKDLGFIVHP
                     YTVNEKADMLRLNKYGVDGVFTNFADKYKEVIK"
     gene            complement(4267..5646)
                     /gene="argH"
                     /locus_tag="ACGEXS_001069"
     CDS             complement(4267..5646)
                     /gene="argH"
                     /locus_tag="ACGEXS_001069"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFF8012088.1"
                     /translation="MSNKAWGGRFEVQPEEWVDDFNASITFDQTLIDQDIEGSIAHAT
                     MLANQGIISQQDSEQIIQGLKSIQHDYHQDQIQFSASLEDIHLNIEHELIKRIGDAGG
                     KLHTGRSRNDQVATDMHLYTKKQVQDIIALIKSLQSVIVDIASNNVDTIMPGYTHLQR
                     AQPISFAHHIMTYFWMLQRDQQRFEDSLKRIDINPLGAAALSGTTYPIDRHETTALLN
                     FGSLYENSLDAVSDRDYIIETLHNISLTMVHLSRFAEEIIFWSTDEAKFITLSDAFST
                     GSSIMPQKKNPDMAELIRGKVGRTTGHLMSMLMTLKGLPLAYNKDMQEDKEGLFDAVH
                     TIKGSLRIFEGMIQTMTINKERLNQTVKEDFSNATELADYLVTKNIPFRTAHEIVGKI
                     VLECIQQGHYLLDVPLATYQQHHSSIDADIYDYLQPENCLKRRQSYGSTGQSSVKQQL
                     DVAKQLLSQ"
     gene            complement(5636..6841)
                     /locus_tag="ACGEXS_001070"
     CDS             complement(5636..6841)
                     /locus_tag="ACGEXS_001070"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFF8012089.1"
                     /translation="MKEKIVLAYSGGLDTSVAVQWLIDKGYDVVACCLDVGEGKDLDI
                     VYKKALDMGAVECHIIDATKEFSDEYVSYAIKGNLMYENAYPLVSALSRPLIAKKLVE
                     IAEKTNSVGIAHGCTGKGNDQVRFEVAIKALNPSLKAFAPAREWAWSREEEIDYAIKH
                     NIPVSINHDSPYSIDQNLWGRANECGILEDPYAAPPEDAFDLTNALEETPDTADEIIL
                     TFDKGIPVQIDGKTYELDDLILTLNALAGKHGIGRIDHVENRLVGIKSREIYEAPAAE
                     VILKAHKALETITLTKDVAHFKPIIEKQFAEQLYNGLWFSPLTDSLKLFIDSTQQYVS
                     GDVRIKLFKGNAIVNGRKSPYTLYDEKLATYTKEDAFNQDAAVGFIDIYGLPTQVNAM
                     LHGGYSNEQ"
     gene            7192..8523
                     /locus_tag="ACGEXS_001071"
     CDS             7192..8523
                     /locus_tag="ACGEXS_001071"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFF8012090.1"
                     /translation="MTHIQLDFSKTLEFFGEHELKQQQEIVKSIHKTIHEGTGAGSDF
                     LGWVDLPVDYDKEEFSRIVEASKRIKENSDVLVVIGIGGSYLGARAAIEMLTSSFRNS
                     NEYPEIVFVGNHLSSTYTKELVDYLADKDFSVNVISKSGTTTEPAVAFRLFKQLVEER
                     YGKEEAQKRIFATTDKEKGALKQLATNEGYETFIVPDDVGGRYSVLTAVGLLPIATAG
                     INIEAMMIGAAKAREELSSDKLEENIAYQYATIRNILYAKGYTTEMLINYEPSMQYFN
                     EWWKQLFGESEGKDFKGIYPSSANYTTDLHSLGQYVQEGRRFLFETVVKVNHPKYDIT
                     IEKDSDDLDGLNYLAGKTIDEVNTKAFEGTLLAHTDGGVPNMVVNIPQLDEETFGYVV
                     YFFELACAMSGYQLGVNPFNQPGVEAYKQNMFALLGKPGFEDLKKELEERL"
     gene            8848..9423
                     /locus_tag="ACGEXS_001072"
     CDS             8848..9423
                     /locus_tag="ACGEXS_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFF8012091.1"
                     /translation="MSFHQVEEWFEIFRQFGYLPGFILLYIRAIIPVFPLALYILINI
                     QAYGPILGILISWLGLISGTFTVYLICKRLVNTERMQRIKQRTAVQRLISFIDRQGLI
                     PLFILLCFPFTPNTLINFVASLSHIRPKYYFIVLASSKLVSTIILGYLGKEITTILTH
                     PLRGILMLVVLVVFWIVGKKLEQHFMGSKKE"
     gene            9428..9952
                     /gene="lepB"
                     /locus_tag="ACGEXS_001073"
     CDS             9428..9952
                     /gene="lepB"
                     /locus_tag="ACGEXS_001073"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFF8012092.1"
                     /translation="MKKVVKYLISLILAIIIVLFVQTFVIVGHVIPNNDMSPTLNKGD
                     RVIVNKIKVTFNQLNNGDIITYRRGNEIYTSRIIAKPGQSMAFRQGQLYRDDRPVDAS
                     YAKNRKIKDFSLRNFKELDGDIIPPNNFVVLNDQDNNKHDSRQFGLIDKKDIIGNVSL
                     RYYPFSKWTVQFKS"
     gene            9968..10543
                     /gene="lepB"
                     /locus_tag="ACGEXS_001074"
     CDS             9968..10543
                     /gene="lepB"
                     /locus_tag="ACGEXS_001074"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFF8012093.1"
                     /translation="MKKEILEWIISIAVAFVILFIVGKFIVTPYTIKGESMDPTLKDG
                     ERVAVNIVGYKTGGLEKGNVVVFHANKNDDYVKRVIGVPGDKVEYKNDTLYVNGKKQD
                     EPYLNYNLKHKQGDYITGTFQVKDLPNANPKSNVIPKGKYLVLGDNREVSKDSRAFGL
                     IDEDQIVGKVSFRFWPFSEFKHNFNPENTKN"
     gene            10703..14179
                     /gene="addB"
                     /locus_tag="ACGEXS_001075"
     CDS             10703..14179
                     /gene="addB"
                     /locus_tag="ACGEXS_001075"
                     /EC_number="3.1.-.-"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HFF8012094.1"
                     /translation="MTLHAYLGRAGTGKSTKMLTEIKQKMKADPLGDPIILIAPTQST
                     FQLEQAFVNDPELNGSLRTEVLHFERLSHRIFQEVGSYSEQKLSKAATEMMIYNIVQE
                     QQKYLKLYQSQAKYYGFSEKLTEQIQDFKKYAVTPEHLEHFIADKNMQTRTKNKLEDI
                     ALIYREFEQRIQNEFITGEDSLQYFIDCMPKSEWLKRADIYIDGFHNFSTIEYLIIKG
                     LIKYAKSVTIILTTDGNHDQFSLFRKPSEVLRHIEEIANELNISIERQYFNQLYRFNN
                     QDLKHLEQEFDALQINRVACQGHINILESATMREEINEIARRIIVDIRDKQLRYQDIA
                     ILYRDESYAYLFDSILPLYNIPYNIDTKRSMTHHPVMEMIRSLIEVIQSNWQVNPMLR
                     LLKTDVLTASYLKSAYLVDLLENFVLERGIYGKRWLDDELFNVEHFSKMGRKAHKLTE
                     DERNTFEQVVKLKKDVIDKILHFEKQMSQAETVKDFATAFYESMEYFELPNQLMTERD
                     ELDLNGNHEKAEEIDQIWNGLIQILDDLVLVFGDEPMSMERFLEVFDIGLEQLEFVMI
                     PQTLDQVSIGTMDLAKVDNKQHVYLVGMNDGTMPQPVTASSLITDEEKKYFEQQANVE
                     LSPTSDILQMDEAFVCYVAMTRAKGDVTFSYSLMGSSGDDKEISPFLNQIQSLFNQLE
                     ITNIPQYHEVNPLSLMQHAKQTKITLFEALRAWLYDEIVADSWLDAYQVIRDSDHLNQ
                     GLDYLMSALTFDNETVKLGETLSKDLYGKEINASVSRFEGYQQCPFKHYASHGLKLNE
                     RTKYELQNFDLGDIFHSVLKYISERINGDFKQLDLKKIRQLTNEALEEILPKVQFNLL
                     NSSAYYRYLSRRIGAIVETTLSALKYQGTYSKFMPKHFETSFRRKPRTNDELIAQTLT
                     TTQGIPINIRGQIDRIDTYTKNDTSFVNIIDYKSSEGSATLDLTKVYYGMQMQMMTYM
                     DIVLQNKQRLGLTDIVKPGGLLYFHVHEPRIKFKSWSDIDEDKLEQDLIKKFKLSGLV
                     NADQTVIDALDIRLEPKFTSDIVPVGLNKDGSLSKRGSQVADEATIYKFIQHNKENFI
                     ETASNIMDGHTEVAPLKYKQKLPCAFCSYQSVCHVDGMIDSKRYRTVDETINPIEAIQ
                     NININDEFGGEQ"
     gene            14180..>17829
                     /gene="addA"
                     /locus_tag="ACGEXS_001076"
     CDS             14180..>17829
                     /gene="addA"
                     /locus_tag="ACGEXS_001076"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFF8012095.1"
                     /translation="MTIPEKPQGVIWTDAQWQSIYATGQDVLVAAAAGSGKTAVLVER
                     IIQKILRDGIDVDRLLVVTFTNLSAREMKHRVDQRIQEASIADPANAHLKNQRIKIHQ
                     AQISTLHSFCLKLIQQHYDVLNIDPNFRTSSEAENILLLEQTIDEVIEQHYDILDPAF
                     IELTEQLSSDRSDDQFRMIIKQLYFFSVANPNPTNWLDQLVTPYEEEAQQAQLIQLLT
                     DLSKVFITAAYDALNKAYDLFSMMDSVDKHLAVIEDERRLMGRVLEGGFIDIPYLTGH
                     EFGARLPNVTAKIKEANEMMVDALEDAKLQYKKYKSLIDKVKSDYFSREADDLKADMQ
                     QLAPRVKYLARIVKDVMSEFNRKKRSKNILDFSDYEHFALQILTNEDGSPSEIAESYR
                     QHFQEILVDEYQDTNRVQEKILSCIKTGDEHNGNLFMVGDVKQSIYKFRQADPSLFIE
                     KYQRFTIDGDGTGRRIDLSQNFRSRKEVLSTTNYIFKHMMDEQVGEVKYDEAAQLYYG
                     APYDESDHPVNLKVLVEADQEHSDLTGSEQEAHFIVEQVKDILEHQKVYDMKTGSYRS
                     ATYKDIVILERSFGQARNLQQAFKNEDIPFHVNSREGYFEQTEVRLVLSFLRAIDNPL
                     QDIYLVGLMRSVIYQFKEDELAQIRILSPNDDYFYQSIVNYINDEAADAILVDKLKMF
                     LSDIQSYQQYSKDHPVYQLIDKFYNDHYVIQYFSGLIGGRGRRANLYGLFNKAIEFEN
                     SSFRGLYQFIRFIDELIERGKDFGEENVVGPNDNVVRMMTIHSSKGLEFPFVIYSGLS
                     KDFNKRDLKQPVILNQQFGLGMDYFDVDKEMAFPSLASVAYRAVAEKELVSEEMRLVY
                     VALTRAKEQLYLIGRVKNDKSLLELEQLSISGEHIAVNERLTSPNPFHLIYSILSKHQ
                     SASIPDDLKFEKDIAQIEDSSRPNVNISIVYFEDVSTETILDNDEYRSVNQLETMQNG
                     NEDVKAQIKHQLDYRYPYVNDTKKPSKQSVSELKRQYETEESGTSYERVRQYRIGFST
                     YERPKFLSEQGKRKANEIGTLMHTVMQHLPFKKERISEVELHQYIDGLIDKHIIEADA
                     KKDIRMDEIMTFINSELYSIIAEAEQVYRELPFVVNQALVDQLPQGDEDVSIIQGMID
                     LIFVKDGVHYFVDYKTDAFNRRRGMTDEEIGTQLKNKYKIQMKYYQNTLQTILNKEVK
                     GYLYFFKFGTLQL"
BASE COUNT         6159 a         2649 c         3267 g         5754 t
ORIGIN      
        1 tatgtcatta taaatgtaag ggttttcaaa atagacaaat ttaatgaaac ttagtgatta
       61 tttcatcttt tttggtggaa aagtatctgc tttgaaaact aatgtaatta tttggataat
      121 gtttcatgta taatagttat aaatgatgaa aaggggagat atgatgacta aatctgaaaa
      181 aattattgag ttaacaaatc attacggagc acataattat ttaccattgc caattgtcat
      241 ttcagaagct gaaggggtat gggttaaaga tcctgaaggc aataaatata tggatatgtt
      301 atctgcatat tccgctgtta accaaggtca tagacatccg aaaattattc aagcattaaa
      361 agatcaagct gataaagtga ctttagtttc acgtgctttt catagtgata acttaggtga
      421 atggtacgaa aaaatttgta aactggcagg taaagataaa gctttaccaa tgaatacagg
      481 tgctgaagca gtagaaacag ctttgaaagc agcacgacgc tgggcatacg atgttaaagg
      541 aattgagcca aataaagcag aaatcattgc atttaatggt aacttccatg gtcgaacaat
      601 ggcgccagtt tcattatctt cagaagcaga ataccaacgt ggttatggtc cgttattaga
      661 tggatttaga aaagttgatt ttggagatgt agatgcattg aaagctgcaa ttaatgaaaa
      721 tactgcagca gttttagtag aaccaattca aggtgaagcg ggtataaata taccgccaga
      781 aggatatttg aaagcaatta gagaattatg tgatgaacat aatgtcttat ttattgctga
      841 cgaaatccaa gcaggattag gtcgttcggg taaattattt gctacggatt gggataatgt
      901 aaaacctgat gtctatattt taggtaaagc actaggtggt ggagtcttcc caatttctgt
      961 tgtattagca gataaagaag tattagatgt ctttacacct ggctcacatg gttcaacatt
     1021 tggtggtaat ccacttgctt gtgctgcatc aattgctgca ttagatgtta tcgttgatga
     1081 ggatttacca ggccgctctt tagaattagg agattatttt aaagaacaat taaagcaaat
     1141 tgatcatcca tcaattaaag aagtccgtgg acgtggtttg tttataggtg tggaacttaa
     1201 tgaaagtgct agaccatatt gtgaagcttt gaaagaagaa ggcttattat gtaaagaaac
     1261 gcatgatact gtcattcgtt ttgcaccacc attaattatt actaaagaag aattggacct
     1321 tgcacttgaa aaaataagac atgtatttca ataatgtcaa aaagttgtaa caatcagttt
     1381 gtaagggctt taaaaaggaa tacgttatgt tacaatatgt ttgaaagcga aatcattagg
     1441 taaagaagag gcgaaaagga tcatgactga gaacaataat ttagtaactt ctactcaagg
     1501 aattattaaa gaagcattgc ataaattggg atttgacgaa ggaatgtacg atttaattaa
     1561 agaaccttta agaatgttac aagtgcgtat ccctgtacga atggatgatg gcacagtaaa
     1621 aacattcaca ggttaccgtg cgcaacataa tgatgctgtt ggaccaacaa aagggggcgt
     1681 gcgtttccac ccagatgttg atgaagaaga agtaaaagca ttatcaatgt ggatgacttt
     1741 gaaatgtggc attgtaaact taccatacgg tggtggtaag ggtggtatcg tttgtgatcc
     1801 acgtcaaatg agcattcatg aagttgaacg tttatcacgc ggatatgtaa gagcaatttc
     1861 acaattcgta ggtccgaaca aagatattcc agcaccagat gtatttacaa actcacaaat
     1921 tatggcttgg atgatggatg aatatagtgc attagataaa tttaattcac caggtttcat
     1981 cacaggtaaa ccaattgtat tgggtggttc tcatggacgc gacagatcaa ctgcactagg
     2041 tgtagttatt gcaattgaac aagctgcaaa acgtcgtaat atgcaaattg aaggtgccaa
     2101 ggttgttatt caaggtttcg gtaatgccgg aagtttctta gctaaattct tatatgattt
     2161 aggtgcaaaa attgtaggta tctctgatgc ttacggtgca ttacacgatc caaatggctt
     2221 agatatagat tatttattag accgtcgtga tagttttggt acggtaacaa atttatttga
     2281 agaaacaatc tcaaataaag aattgtttga attagattgt gacattttag taccagcggc
     2341 tatttcaaac caaattacag aagacaatgc acatgatatt aaagctagta tcgttgttga
     2401 agctgctaat ggacctacaa caccagaagc aacacgtatt ttaactgaac gtggtatatt
     2461 attagttcca gacgtattag caagtgctgg tggtgtaacg gtttcttact tcgaatgggt
     2521 acaaaataat caaggttatt attggtctga agaagaagtt aatgaaaagc tacgtgaaaa
     2581 attagaagcg gcatttgata cgatttacga attgtctcaa aaccgaaaaa tagatatgag
     2641 acttgcagca tatatcatag gtattaaacg tacagcagaa gcagctagat atcgtggttg
     2701 ggcataatta attatcatat gtgatttaac gagcttggga cagaaaacaa agccctaagc
     2761 tcgttaattt tattttagca gtagttgact gtaaaacaat gcccgtgtaa cacgctcttt
     2821 tcaaaaatag tcggggcccc aacacagagg ctggtggaaa gtcagcttac aatagtgtgc
     2881 aagttggcgg ggccccaaca cagaggctgg cggaaagtca gcttacaata gtgtgcaagt
     2941 tggggtggga cgacgaaata aattttgcga aaatatcatt tctgtcccac tcccaaaaag
     3001 acagcagtag gataatttca atttggaaat atcttactgc tgttttttat agtaatagct
     3061 atatttttgt atttatgttt tctagtttaa cattactact taatgacttc tttatattta
     3121 tcagcgaaat ttgtaaagac accatcaacg ccatatttat ttaatcgtaa catatcagct
     3181 ttttcattca ctgtataagg atgtactata aatcctaagt cttttaaatg atgggtattt
     3241 tgttcagtta aatctgtata atcaggacct aatccaatcg cataagagcg tatctctttt
     3301 aagcgttggt cgttaaattg ttgtagttca cctttatcaa ctaattttac taatggcaca
     3361 tgcttatttt gacgatgaat tttctttaaa ctttcgtcag aaaatgattg aatcattaca
     3421 tgtccatttt ttaatttatt gttatttaaa aggtgatgct ttttcaatga agctaataat
     3481 tgttcttcca ttcctgggta tacatcagat gactttgttt caatataata gtttgcattc
     3541 gggccataac gttctaaaat ttcatctaaa gtgggtactt tagcattttt ataacttgct
     3601 cttgcgtatt ttggatattt tttattaaac caacttcctg catctaactg ttttaattca
     3661 tcaagggtat aatcctcaac tttaccgtgt ccatttgttg tacggttaac agtttcatca
     3721 tgcatagcaa ctaaatggcc atctttggta cgttgtaaat caatttcgat ataagatgct
     3781 tttaactcat tatgactctt atcatatgct tgaaacgtat gctcgggtgc atagccactt
     3841 gcgccacgat gtgcgatagt agtgaatcgc tcattcgtta aatttgtatg ccattgaata
     3901 gcctgaggtt tatttgcaat ttgatttgtt tgttcagcgc cagcagtagg tactgataaa
     3961 aatcccatag taaaaacagc agaagcagcc ataaatttag tgaagctttt cgaagagtta
     4021 gtcatagtct ttatcctttc atctctttat ttgtagtcta acaattttaa aatactacaa
     4081 aaacctgctt gtgaaaacgc tttacacatt tcttttacaa tttataacat ttttgtaata
     4141 taaatttaaa agaattatca aacattttag gaatcttaaa aatgtgatgt tcaacaagac
     4201 gaagagagca ttaaaatacc cacaatgtaa ttttaataga cattgtgggt aggtagatta
     4261 acgtatttat tgtgatagta attgtttagc aacatcaagt tgttgtttga ccgatgattg
     4321 acctgttgaa ccgtaacttt gacgtcgttt taaacaattt tcaggctgca aataatcgta
     4381 aatatcggca tcaatactag aatgatgttg ttgatatgtt gctaaaggaa catctaataa
     4441 ataatgacct tgttgtatac attctaagac gatttttcct acaatttcat gtgcagttct
     4501 aaatggaata tttttagtta ctaaataatc tgctagttcc gttgcatttg aaaaatcttc
     4561 tttaacagtt tgattgagtc gttctttatt aattgtcatc gtttgaatca taccttcgaa
     4621 aatacgtaaa gaacctttaa ttgtatggac agcatcgaat aaaccttctt tatcttcctg
     4681 catatctttg ttatatgcta gaggtaatcc ttttaaagtc ataagcatgc tcattaaatg
     4741 accagtcgtt cgaccaactt tacctctaat taattctgcc atatcaggat ttttcttttg
     4801 tggcataata gatgagccag ttgaaaatgc atctgataat gtaatgaatt tagcttcgtc
     4861 tgtggaccag aaaataattt cctctgcaaa gcgtgataag tgaaccatcg ttaaagaaat
     4921 attatgcaat gtttcaataa tatagtctct gtcactaaca gcatctaggc tattctcata
     4981 gagactgcca aagttcaaca atgctgttgt ctcgtgtcta tcgatagggt atgtggtacc
     5041 acttaaggct gctgcaccta aaggattaat atcgattcgt tttaaactat cttcaaatcg
     5101 ttgttggtct cgttgtaaca tccaaaaata agtcataata tgatgtgcaa atgaaattgg
     5161 ctgtgcacgc tgtaaatgag tataaccagg cataattgta tcaacattat tggaagcgat
     5221 gtctacaatt acactttgta acgacttaat taatgcgatg atatcttgca cttgtttctt
     5281 agtgtacaag tgcatgtctg ttgcaacttg atcgtttcta ctgcgtccag tatgcaactt
     5341 accaccagca tcaccgatac gtttaattaa ttcatgttca atatttaaat gaatatcttc
     5401 taatgatgca ctaaattgaa tttgatcttg atgataatca tgttgaatag attttagtcc
     5461 ttgtataatt tgttcgctgt cttgttgact aataatgcct tgattcgcaa gcatagttgc
     5521 atgtgcaatg ctgccttcga tatcttgatc tatgagcgtt tgatcaaaag taatggatgc
     5581 gttaaagtcg tcaacccact cttcaggttg tacttcaaat ctaccgcccc aagctttatt
     5641 gctcattgct atagcctcca tgcaacatcg catttacttg agtaggtaaa ccatagatat
     5701 cgataaagcc aacagcagcg tcttgattaa atgcatcttc ttttgtataa gttgctaatt
     5761 tttcatcata taatgtgtaa ggtgattttc taccattcac gatggcatta cctttgaata
     5821 atttaattct gacatcacca cttacgtatt gctgagtact atcaataaat aatttcaagc
     5881 tatcagttaa aggtgagaac caaagtccat tgtatagttg ttcagcaaat tgcttctcaa
     5941 tgattggttt aaagtgtgcg acatctttcg ttaacgtaat cgtttctaat gctttatgcg
     6001 cttttaaaat aacttctgca gcaggtgcct cataaatttc tcttgatttg atacctacaa
     6061 gtctattttc tacatggtca attcttccga taccatgctt accagctaat gcattcaacg
     6121 ttaaaattaa atcgtctaat tcatatgttt tgccatcaat ttgaactggg atgcctttat
     6181 caaacgttaa aatgatttca tcagcagtat ctggtgtttc ttctaaagca tttgttagat
     6241 cgaacgcatc ctctggtggc gcagcataag gatcttctaa aataccacat tcattcgctc
     6301 tgccccatag attttgatcg atagaataag gtgaatcatg gttgattgat acagggatat
     6361 tatgtttaat tgcataatcg atttcttctt cacgactcca tgcccactca cgtgcaggtg
     6421 cgaatgcttt caatgatggg tttaatgctt taatggcaac ttcgaaacgt acttggtcat
     6481 tccctttacc agtacaacca tgtgcaatac ctactgaatt tgttttctca gcaatctcta
     6541 ctaatttttt agcgattaat ggtcttgata aagctgaaac taatggatat gcattttcat
     6601 acattaaatt tcctttgatt gcataactta catactcatc actaaattct tttgttgcat
     6661 caataatatg acattcaact gctcccatat ctaaagcttt tttataaacg atgtctaaat
     6721 ctttaccttc accaacatct aggcaacaag ctacaacgtc gtatcctttg tcgataagcc
     6781 attgaacggc cacacttgta tctagtcctc ctgaatatgc taaaacaatt ttctctttca
     6841 taaaattcac ctcattgtag ttatctgaat atttcgtatg aacacatgat agcaaacttt
     6901 taatgaaaat aaaaccatta ttttacatat ttattcataa aatgcgatag agatagttag
     6961 agttgtaagt gtaaatgctt atttatcaat gtttaaaagt attttaaaaa acatgcaaat
     7021 tgtataaaaa atgtttgagt gtttgacgcc agtttgaaat gtttgttgaa aaataggtgt
     7081 gaaacgtcac ataaatatca attttattga ttaagtacaa acttgtgaat tgaatcgcaa
     7141 acgtaaactt agtaaaatta agatattaaa tgaaacagga ggcttttata gatgactcat
     7201 attcaattag attttagtaa aacgttagaa tttttcggtg aacacgaatt aaaacaacaa
     7261 caagaaattg ttaaatcaat tcacaaaaca attcatgaag gtactggtgc aggtagtgac
     7321 ttcttaggct gggttgattt accagttgat tacgacaaag aagaattttc aagaattgtt
     7381 gaagcatcaa aacgcattaa agaaaattct gatgttttag tagtcatcgg tattggtggt
     7441 tcttacttag gtgcacgtgc agcaatcgaa atgttaacgt catcatttag aaacagcaat
     7501 gaataccctg aaattgtatt tgttggtaat cacttatcat caacatatac gaaagagtta
     7561 gttgattatt tagcagacaa agatttctct gtaaacgtta tttctaaatc tggtacaact
     7621 acagaaccag cagttgcatt tagattgttc aaacaattag ttgaagaaag atacggtaaa
     7681 gaagaagcac aaaaacgtat atttgcaaca acggataaag aaaaaggtgc tttaaaacag
     7741 ttggctacaa acgaaggtta tgaaacgttt atcgtacctg atgatgtagg tggaagatat
     7801 tctgttttaa cagcagtagg attattacca attgcaacag ctggaattaa catcgaagct
     7861 atgatgattg gtgctgcaaa agcacgtgaa gaattatctt cagataaatt agaagaaaac
     7921 attgcatacc aatatgcgac aattcgaaac attttatatg caaaaggtta tacaacagaa
     7981 atgttgatta actatgaacc atctatgcaa tactttaatg aatggtggaa acaattattt
     8041 ggtgaatcag aaggtaaaga cttcaaaggt atctatcctt caagtgccaa ctacacaact
     8101 gatttacatt ctttaggtca atatgtacaa gaaggccgtc gcttcttatt cgaaacagtt
     8161 gtaaaagtaa atcatcctaa atatgatatt actattgaaa aagatagtga tgatctagac
     8221 ggattaaatt atttagctgg taaaacaatc gacgaagtta acacaaaagc attcgaaggt
     8281 acattattag cgcatactga tggtggtgtt cctaacatgg tagtgaacat tccacaatta
     8341 gatgaagaaa ctttcggtta cgtcgtatac ttcttcgaac ttgcttgtgc aatgagtgga
     8401 taccaattag gcgtaaatcc atttaaccaa cctggtgtag aagcatataa acaaaacatg
     8461 ttcgcattat taggtaaacc tggttttgaa gacttgaaaa aagaattaga agaacgttta
     8521 taaaatacat tacttcaaag attagtgaag tttgaaaaga tagaactaga cgttaactat
     8581 ttaaagcata ttttcgaggt tgtcattaca aatgtaaaaa tgtaatgaca acctcgtttt
     8641 tatttatatg caagaactag gttactagct aatgtgacaa gatgttaaga gaaaattaaa
     8701 gataaaataa tatctgcctt acaataatat tgttatacta ctagagactg atttattagc
     8761 atgattacat gttaatgttt ctttacttag taattaactt tataatgtaa gaataattat
     8821 cttcaaccaa agaaagggat tgatgatttg tcgtttcatc aagtagaaga atggtttgag
     8881 atatttcgac agtttggtta tttacctgga tttatattgt tatatattag agctataatt
     8941 ccagtatttc ctttagcact ctatatttta attaacattc aagcttatgg acctatttta
     9001 ggtatattga ttagttggct tggattaatt tctggaacat ttacagtcta tttgatctgt
     9061 aaacgattgg tgaacactga gaggatgcag cgaattaaac aacgtactgc tgttcaacgc
     9121 ttgattagtt ttattgatcg ccaaggatta atcccattgt ttattttact ttgttttcct
     9181 tttacgccaa atacattaat aaattttgta gcgagtctat ctcatattag acctaaatat
     9241 tatttcattg ttttggcatc atcaaagtta gtttcaacaa ttattttagg ttatttaggt
     9301 aaggaaatta ctacaatttt aacgcatcct ttaagaggga tattaatgtt agttgtgttg
     9361 gttgtatttt ggattgttgg aaaaaagtta gaacagcatt ttatgggatc gaaaaaggag
     9421 tgacatcgtg aaaaaagttg taaaatattt gatttcattg atacttgcta ttatcattgt
     9481 actgttcgta caaacttttg taatagttgg tcatgtcatt ccgaataatg atatgtcacc
     9541 aacccttaac aaaggggatc gtgttattgt aaataaaatt aaagttacat ttaatcaatt
     9601 gaataatggt gatatcatta catataggcg tggtaacgag atatatacta gtcgaattat
     9661 tgccaaacct ggtcaatcaa tggcgtttcg tcagggacaa ttataccgtg atgaccgacc
     9721 ggttgacgca tcttatgcca agaacagaaa aattaaagat tttagtttgc gcaattttaa
     9781 agaattagat ggtgatatta ttccgccaaa caattttgtt gtgctaaatg atcaagataa
     9841 taacaagcac gattcaagac aatttggttt aatcgataaa aaggatatta ttggtaatgt
     9901 tagtttacga tactatcctt tttcaaaatg gactgttcag ttcaaatctt aaaaagaggt
     9961 gtcaaaattg aaaaaagaaa tattggaatg gattatttca attgcagtcg cttttgtcat
    10021 tttatttata gtaggtaaat ttattgttac gccatataca attaaaggtg aatcaatgga
    10081 tccaactttg aaagatggcg agcgagtagc tgtaaacatt gttggatata aaacaggtgg
    10141 tttggaaaaa ggtaatgtag ttgtcttcca tgcaaacaaa aatgatgact atgttaaacg
    10201 tgtcatcggt gttcctggtg ataaagtaga atacaaaaat gatacattat atgtcaatgg
    10261 taaaaaacaa gatgaaccat atttaaacta caatttaaaa cataaacaag gtgattacat
    10321 tactgggact ttccaagtta aagatttacc gaatgcgaat cctaaatcaa atgtcattcc
    10381 aaaaggtaaa tatttagtgc ttggagataa tcgtgaagta agtaaagata gccgtgcgtt
    10441 tggcctcatt gatgaagacc aaattgttgg taaagtttca tttaggttct ggccatttag
    10501 tgaatttaaa cataatttca atcctgaaaa tactaaaaat taatatgaaa caaatacaac
    10561 atcgtttgtc ggttttaata ctgataaacg atgttttatt ttgttagtac cacaataaaa
    10621 gctaagttcg aaatgaactt ataataaatc aatcacaatc actttgtgtt aaaatatgtg
    10681 tcaaaggaag tgagggtttg tcatgacatt acatgcttat ttaggtagag cgggaacagg
    10741 taagtctacg aaaatgttga ccgaaataaa acaaaaaatg aaagcagatc cgcttggaga
    10801 tccaatcatt ttaattgcgc caactcaaag tacatttcaa ttagaacaag cctttgtcaa
    10861 tgatccggaa ttaaatggta gtttaagaac agaagtgttg cattttgaac gattaagtca
    10921 tcgtattttc caagaagttg gtagttatag cgaacaaaag ttatctaaag ctgcaacgga
    10981 aatgatgatt tataacattg ttcaagaaca acaaaagtat ttaaaacttt atcaatcaca
    11041 agcaaaatat tatgggttta gtgaaaaatt aacagaacaa attcaagatt ttaaaaaata
    11101 tgcagtaacg cctgaacatt tagaacactt tattgctgat aaaaatatgc aaactcgaac
    11161 taaaaataag ttagaggata ttgctttaat ataccgtgag ttcgaacaac gcattcaaaa
    11221 cgagtttatt acgggtgagg attcattaca atattttatt gattgtatgc cgaaatcaga
    11281 gtggctaaaa cgtgctgata tatatattga tggttttcac aacttttcaa cgattgagta
    11341 tttaataatc aaaggattaa ttaaatatgc gaagagtgtc acaattatat tgacgacaga
    11401 tggtaaccac gatcaattta gtttatttag aaaaccatcg gaagtgttac gacatattga
    11461 agaaatagca aatgaactca atatttctat tgaacgtcaa tatttcaacc aattatatcg
    11521 cttcaataat caagatttaa agcatcttga acaagaattt gatgcacttc aaatcaatcg
    11581 agtggcatgt caaggtcata tcaatatttt agaatctgcg actatgagag aggaaataaa
    11641 tgaaattgcg cgacgtatca tcgttgatat tcgtgataag caattacgat atcaagatat
    11701 tgctatttta tatcgtgatg aatcttatgc ttatttattt gattccatat taccgcttta
    11761 taatattcct tataatattg atacaaagcg ttcgatgaca catcatccgg tcatggaaat
    11821 gattcgttca ttgattgaag ttattcaatc taattggcaa gtgaatccaa tgctacgctt
    11881 attgaagact gatgtgttaa cggcatcata tctaaaaagt gcatacttag ttgatttact
    11941 tgaaaatttt gtacttgaac gtggtatata cggtaaacgt tggttagatg atgagctatt
    12001 taatgtcgaa cattttagca aaatggggcg taaagcgcat aaactgaccg aagatgaacg
    12061 taacacattt gaacaagtcg ttaagttaaa gaaagatgtc attgataaaa ttttacattt
    12121 tgaaaagcaa atgtcacaag cggaaactgt aaaagatttt gcaactgctt tttatgaaag
    12181 tatggaatat ttcgaactgc caaatcaatt gatgacagag cgagatgaac ttgatttaaa
    12241 tggtaatcat gaaaaggcgg aggaaattga tcaaatatgg aatggcttaa ttcaaatcct
    12301 tgatgactta gttctagtat ttggagatga accaatgtcg atggaacgtt tcttagaagt
    12361 atttgatatt ggtttagaac aattagaatt tgttatgatt ccgcaaacat tggaccaagt
    12421 aagtattggt acgatggatt tggctaaagt cgataataag caacatgttt acttagtagg
    12481 tatgaatgat ggaacgatgc cacaaccagt aactgcgtca agcttgatta cagatgaaga
    12541 aaagaaatac tttgaacagc aggctaatgt cgagttaagt ccaacatcag atattttaca
    12601 gatggatgaa gcatttgttt gttatgttgc tatgactaga gctaagggag atgttacatt
    12661 ttcttacagt ctaatgggat caagtggtga tgataaggag atcagcccat ttttaaatca
    12721 aattcaatca ttgttcaacc aattggaaat tactaacatt cctcaatacc atgaagttaa
    12781 cccattgtca ctaatgcaac atgctaagca aaccaaaatt acattatttg aagcattgcg
    12841 tgcttggtta tatgatgaaa ttgtggctga tagttggtta gatgcttatc aagtaattag
    12901 agatagcgat catttaaatc aaggtttaga ttatttaatg tcagcattaa cgtttgacaa
    12961 tgaaactgta aaattaggtg aaacgttgtc taaagattta tatggtaagg aaatcaatgc
    13021 cagtgtatcc cgttttgaag gttatcaaca atgcccattt aaacactatg cgtcacatgg
    13081 tctgaaacta aatgagcgaa cgaagtatga acttcaaaac tttgatttag gtgatatttt
    13141 ccattctgtt ttaaaatata tatctgaacg tattaatggc gattttaaac aattagacct
    13201 gaaaaaaata agacaattaa cgaatgaagc attggaagaa attttaccta aagttcagtt
    13261 taatttatta aattcttcag cttactatcg ttatttatca agacgcattg gcgctattgt
    13321 agaaacaaca ctaagcgcat taaaatatca aggcacgtat tcaaagttta tgccaaaaca
    13381 ttttgagaca agttttagaa ggaaaccaag aacaaatgac gaattaattg cacaaacatt
    13441 aacgacaact caaggtattc caattaatat tagagggcaa attgaccgta tcgatacgta
    13501 tacaaagaat gatacaagtt ttgttaatat cattgactat aaatcctctg aaggtagtgc
    13561 gacacttgat ttaacgaaag tatattatgg tatgcaaatg caaatgatga catacatgga
    13621 tatcgtttta caaaataaac aacgccttgg attaacagat attgtgaaac caggtggatt
    13681 attatacttc catgtacatg aacctagaat taaatttaaa tcatggtctg atattgatga
    13741 agataaacta gaacaagatt taattaaaaa gtttaagttg agtggtttag ttaatgcaga
    13801 ccaaactgtt attgatgcat tggatattcg tttagaacct aaattcactt cagatattgt
    13861 accagttggt ttgaataaag atggctcttt gagtaaacga ggcagccaag tggcagatga
    13921 agcaacgatt tataaattca tccaacataa caaagagaat tttatagaaa cagcttcaaa
    13981 tattatggat ggacatactg aagttgcacc attaaagtac aaacaaaaat tgccatgtgc
    14041 tttttgtagt tatcaatcgg tatgtcatgt agatggcatg attgatagta agcgatatcg
    14101 aactgtagat gaaacaataa atccaattga agcaattcaa aatattaaca ttaatgatga
    14161 atttgggggt gagcaataga tgacaattcc agagaaacca caaggcgtga tttggactga
    14221 cgcgcaatgg caaagtattt acgcaactgg acaagatgta cttgttgcag ccgcggcagg
    14281 ttcaggtaaa acagctgtac tagttgagcg tattatccaa aagattttac gtgatggcat
    14341 tgatgtcgat cgacttttag tcgtaacgtt tacaaactta agcgcacgtg aaatgaagca
    14401 tcgtgtagac caacgtattc aagaggcatc gattgctgat cctgcaaatg cacacttgaa
    14461 aaaccaacgc atcaaaattc atcaagcaca aatatctaca ctccatagtt tttgcttgaa
    14521 attaattcaa cagcattatg atgtattaaa tattgacccg aactttagaa caagcagtga
    14581 agctgaaaat attttattat tagaacaaac gatagatgag gtcatagaac aacattacga
    14641 tatccttgat cctgctttta ttgaattaac agagcaattg tcttcagata gaagtgatga
    14701 tcagtttcga atgattatta aacaattgta tttctttagc gttgcaaatc caaatcctac
    14761 aaattggttg gatcaattgg tgacaccata cgaagaagaa gcacaacaag cgcaacttat
    14821 tcaactacta acagacttat ctaaagtatt tatcacagct gcttatgatg ctttaaataa
    14881 ggcgtatgat ttgtttagta tgatggatag cgtcgataaa catttagctg ttatagaaga
    14941 tgaacgacgt ttaatggggc gtgttttaga aggtggcttt attgatatac cttatttaac
    15001 tggtcacgaa tttggcgcgc gtttgcctaa tgtaacagcg aaaattaaag aagcaaatga
    15061 aatgatggtc gatgccttag aagatgctaa acttcagtat aaaaaatata aatcattaat
    15121 tgataaagtg aagagtgatt acttttcaag agaagctgat gatttgaaag ctgatatgca
    15181 acaattggcg ccacgagtaa agtaccttgc gcgtattgtg aaagatgtta tgtcagaatt
    15241 caatcgaaaa aagcgtagca aaaatatttt ggatttttct gattatgaac attttgcatt
    15301 acaaatttta actaatgagg atggttcgcc ttcagaaatt gccgaatcat accgtcaaca
    15361 cttccaagaa atattggtcg atgagtatca agatacgaac cgagttcaag agaaaatact
    15421 atcttgcatc aaaacgggtg atgaacataa tggtaattta tttatggttg gagatgttaa
    15481 gcaatccatt tataaattta gacaagctga tccaagttta tttattgaaa agtatcaacg
    15541 ctttactata gatggagatg gcactggacg tcgaattgat ttgtcgcaaa acttccgttc
    15601 tcgaaaagaa gtactgtcaa cgactaacta tatattcaaa catatgatgg atgaacaagt
    15661 cggtgaagta aaatatgatg aagcggcaca gttgtattat ggtgcaccat atgatgaatc
    15721 ggaccatcca gtaaacttaa aagtccttgt tgaagcggat caagaacata gtgatttaac
    15781 tggtagtgaa caagaagcgc attttatagt agaacaagtt aaagatatct tagaacatca
    15841 aaaagtttat gatatgaaaa caggaagcta tagaagtgcg acatacaagg atatcgttat
    15901 tctagaacgc agctttggac aagctcgcaa tttacaacaa gcctttaaaa atgaagatat
    15961 tccattccat gtgaatagtc gtgaaggtta ctttgaacaa acagaagtcc gcttagtatt
    16021 atcattttta agagcgatag ataatccatt acaagatatt tatttagttg ggttaatgcg
    16081 ctccgttata tatcagttca aagaagacga attagctcaa attagaatat tgagtccaaa
    16141 tgatgactac ttctatcaat cgattgtaaa ttacattaat gacgaagcag cagatgcaat
    16201 tttagttgat aaattaaaaa tgtttttatc agatattcaa agttaccaac aatatagtaa
    16261 agatcatccg gtgtatcagt taattgataa attttataat gatcattatg ttattcaata
    16321 ctttagtgga cttattggtg gacgtggacg acgtgcaaat ctttatggtt tatttaataa
    16381 agctatcgag tttgagaatt caagttttag aggtttatat caatttattc gttttatcga
    16441 tgaattgatt gaaagaggca aagattttgg tgaggaaaat gtagttggtc caaacgataa
    16501 tgtcgttaga atgatgacaa ttcatagtag taaaggtcta gagtttccat ttgtcattta
    16561 ttctggattg tcaaaagatt ttaataaacg tgatttgaaa caaccagtta ttttaaatca
    16621 gcaatttggt ctcggaatgg attattttga tgtggataaa gaaatggcat ttccatcttt
    16681 agcttcggtt gcatatagag ctgttgccga aaaagaactt gtgtcagaag aaatgcgatt
    16741 agtctatgta gcattaacaa gagcgaaaga acaactttat ttaattggta gagtgaaaaa
    16801 tgataaatca ttactagaac tagagcaatt gtctatttct ggtgagcaca ttgctgtcaa
    16861 tgaacgatta acttcaccaa atccgttcca tcttatttat agtattttat ctaaacatca
    16921 atctgcgtca attccagatg atttaaaatt tgaaaaagat atagcacaaa ttgaagatag
    16981 tagtcgtccg aatgtaaata tttcaattgt gtactttgaa gatgtgtcta cagaaaccat
    17041 tttagataat gatgaatatc gttcggttaa tcaattagaa actatgcaaa atggtaatga
    17101 agatgttaaa gcacaaatta aacaccaact tgattatcga tatccatatg taaatgatac
    17161 taaaaagccc tcaaaacaat ctgtttctga attgaaaaga caatatgaaa cagaagaaag
    17221 tggcacaagt tacgaacgag taaggcaata tcgtatcggt ttttcaacgt atgaacgacc
    17281 taaatttcta agtgaacaag gtaaacgaaa agcgaatgaa attggtacgt taatgcatac
    17341 agtgatgcaa catttaccat tcaaaaaaga acgcatatct gaagttgagt tacatcagta
    17401 tatcgatgga ttaatcgata aacatattat cgaagcagat gcgaaaaaag atatccgtat
    17461 ggatgaaata atgacattta tcaatagtga gttatattcg attattgctg aagcagagca
    17521 agtttatcgt gaattaccgt ttgtagttaa ccaagcatta gttgaccaat tgccacaagg
    17581 agacgaagac gtctcaatta ttcaaggtat gattgactta atctttgtta aagatggtgt
    17641 gcattatttt gtagactata aaaccgatgc atttaatcgt cgccgtggga tgacagatga
    17701 agaaattggt acacaattaa aaaataaata taagatacag atgaaatatt atcaaaatac
    17761 gcttcaaacg attcttaata aagaagttaa aggttattta tacttcttca aatttggtac
    17821 attgcaact
//
LOCUS       DAXWFK010000041        16903 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.041, whole genome shotgun sequence.
ACCESSION   DAXWFK010000041 DAXWFK010000000
VERSION     DAXWFK010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16903
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.041"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            121..1002
                     /locus_tag="ACGEXS_001077"
     CDS             121..1002
                     /locus_tag="ACGEXS_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFF8012096.1"
                     /translation="MSIVALLIGLGPLIGWGFFPTVASKFGGKPVHQIIGATVGTLIF
                     AIILAVVTSSGFPTGTNLLFALLSGAGWGFGQIITFKAFELVGSSRAMPVTTAFQLLG
                     ASLWGVFALGNWPGIGHKIIGFTALVVILIGARMTVWSERKEASNAKNLRRAVVLLLI
                     GEFGYWLYSAAPQATSIDGLTAFLPQAMGMVIVAVIYGFMNMKAENPFRNKITWLQII
                     SGFFFAFGALTYLISAQPNMNGLATGFILSQTSVVLATLTGIYFLKQHKTSKEMVITI
                     IGLVLILVAASVTVFIK"
     gene            1017..1421
                     /gene="rbsD"
                     /locus_tag="ACGEXS_001078"
     CDS             1017..1421
                     /gene="rbsD"
                     /locus_tag="ACGEXS_001078"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFF8012097.1"
                     /translation="MKKSAVLNEHISKAIATIGHFDLLTINDAGMPIPNDHRRIDLAV
                     TKNLPRFIDVLATVLEEMEIQKIYLAEEIKEHNPTQLQQIKQLISSEIEIIFIPHEEM
                     KSNLAHPLNKGNIRTGETTPYSNIALESNVTF"
     gene            1449..2363
                     /gene="rbsK"
                     /locus_tag="ACGEXS_001079"
     CDS             1449..2363
                     /gene="rbsK"
                     /locus_tag="ACGEXS_001079"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFF8012098.1"
                     /translation="MTNKVVILGSTNVDQFLTVERYAQPGETLHVEEAQKAFGGGKGA
                     NQAIATARMQADTTFITKIGTDGVADFILEDFKVAHIDTSYIIKTAEAKTGQAFITVN
                     AEGQNTIYVYGGANMTMTPEDVINAKDAIINADFVVAQLEVPIPAIISAFEIAKAHGV
                     TTVLNPAPAKALPNELLSLIDIIVPNETEAELLSGIKVTNEQSMKDNANYFLSIGIKT
                     VLITLGKQGTYFATKNQSQHIEAYKVNAIDTTAAGDTFIGAFVSRLNKSQDNLADAID
                     FGNKASSLTVQKHGAQASIPLLEEVNQV"
     gene            complement(2461..2595)
                     /locus_tag="ACGEXS_001080"
     CDS             complement(2461..2595)
                     /locus_tag="ACGEXS_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012099.1"
                     /translation="MIIIIINIAYLYAIIWKLKRLQKIVTSFITKHESTSIGLVENYF
                     "
     gene            2614..3375
                     /locus_tag="ACGEXS_001081"
     CDS             2614..3375
                     /locus_tag="ACGEXS_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFF8012100.1"
                     /translation="MSKEAGHTFLAKLGKTRLRPGGKEATDWLIQQGAFSQDKQVLEV
                     ACNMCTTSIYLAHTYGCHIQGVDINKKALEKAQENISAAGLESYIQVQQANAVKLPFD
                     DNQFDIVLNEAMLTMLPIAIKEKALREYYRVLKPGGILLTHDIVIVNESHATHVVKSL
                     SAAINVNVSPQTKLGWLDLYNQAGFNHVHYHTGPMSLMTPKGLIYDEGIVGTIKIINN
                     ALKKENRPMFCKMFKTMTKLRKDMNYITFVAKKEH"
     gene            complement(3809..5245)
                     /locus_tag="ACGEXS_001082"
     CDS             complement(3809..5245)
                     /locus_tag="ACGEXS_001082"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFF8012101.1"
                     /translation="MTKLPQNFMWGGALAANQFEGGYDKGGKGLSVIDVMTSGAHGKA
                     RQITESIDPNHYYPNHEGIDFYHRYKEDIALFKEMGLKCLRTSIAWTRIFPNGDEDVP
                     NEEGLAFYDRIFDELIAQGIEPVVTLSHFEMPLHLAKHYGGFRNREVVDYFVHFARVV
                     FERYKDKVTYWMTFNEINNQMDTSNPIFLWTNSGVALTENDNPEEVLYQVAHHELLAS
                     ALAVRLGKEINPKFKIGTMISHVPIYPYSCHPKDMMEAQIANRLRFFFPDVQVRGYYP
                     SYAKKMLARKGYDVGWQEGDDSILQQGTVDYIGFSYYMSTAVKHDVDTTVENNIVNGG
                     LNHSVENPHIATSDWGWAIDPDGLRYTLNVLYDRYQLPLFIVENGFGAVDEVVDGHIH
                     DDYRIEYLKAHITAAIEAVDQDGVDLIGYTPWGIIDIVSFTTGEMKKRYGLIYVDRDN
                     DGHGTMERLKKDSFYWYQQVIASNGDKL"
     gene            complement(5261..6052)
                     /locus_tag="ACGEXS_001083"
     CDS             complement(5261..6052)
                     /locus_tag="ACGEXS_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFF8012102.1"
                     /translation="MSNKYKEQAQDILTAVGGVENIVDATYDTKCITIHMQHTIPSTA
                     NEVKQIVDVTSVAENDTQLVIKLNGNVDEVYQQLQRLIKNANVEESENTDNINSQDTS
                     YTPQVKVTTPILVKAPIAGRRILLKEVRDSIFREKMVGEGLAIKAHEESKVIAPFNGL
                     ISMIVPTKHAVGIQSEDGVDIVIHIGVNTVDLEGKGFKCFVKQNDHVEAGQTLLQFDQ
                     QYIQQQGYNADVIVVISNSADLGKVELTMNEIITTEDVIFKIFKN"
     gene            6201..6905
                     /locus_tag="ACGEXS_001084"
     CDS             6201..6905
                     /locus_tag="ACGEXS_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8012103.1"
                     /translation="MLKYEHIAKQLNAFIHQSNFKPGDKLPSVTQLKERYQVSKSTII
                     KALGLLEQDGLIYQAQGSGIYVRNIADANRINVFKTNGFSKSLGEHRMTSKVLVFKEI
                     ATPPKSVQDELQLNADDTVYYLERLRFVDDDVLCIEYSYYHKEIVKYLNDDIAKGSIF
                     DYLESNMKLRIGFSDIFFNVDQLTSSEASLLQLSTGEPCLRYHQTFYTMTGKPFDSSD
                     IVFHYRHAQFYIPSKK"
     gene            complement(7013..7714)
                     /gene="lrgB"
                     /locus_tag="ACGEXS_001085"
     CDS             complement(7013..7714)
                     /gene="lrgB"
                     /locus_tag="ACGEXS_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like protein LrgB"
                     /protein_id="HFF8012104.1"
                     /translation="MINHLALNTPYFGILLSVIPFFLATILFEKTNRFFLFAPLFVSM
                     VFGVAFLYLTGIPYKTYKIGGDIIYFFLEPATICFAIPLYKKREVLVKHWHRIIGGIG
                     IGTVVALLIILTFAKLAQFANDVILSMLPQAATTAIALPVSAGIGGIKELTSLAVILN
                     GVIIYALGNKFLKLFRITNPIARGLALGTSGHTLGVAPAKELGPVEESMASIALVLVG
                     VVVVAVVPVFVAIFF"
     gene            complement(7707..8150)
                     /gene="lrgA"
                     /locus_tag="ACGEXS_001086"
     CDS             complement(7707..8150)
                     /gene="lrgA"
                     /locus_tag="ACGEXS_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like murein hydrolase modulator LrgA"
                     /protein_id="HFF8012105.1"
                     /translation="MVVKQQKDASKPAHFFHQVIVIALVLFVSKIIESFMPIPMPASV
                     IGLVLLFVLLCTGAVKLGEVEKVGTTLTNNIGLLFVPAGISVVNSLGVISQAPFLIIG
                     LIIVSTILLLICTGYVTQIIMKVTSRSKGDKVTKKIKIEEAQAHD"
     gene            complement(8263..9003)
                     /locus_tag="ACGEXS_001087"
     CDS             complement(8263..9003)
                     /locus_tag="ACGEXS_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor LytR"
                     /protein_id="HFF8012106.1"
                     /translation="MKALIIDDEPLARNELTYLLNEIGGFEEINEAENVKETLEALLI
                     NQYDIIFLDVNLMDENGIELGAKIQKMKEPPAIIFATAHDQYAVQAFELNATDYILKP
                     FGQKRIEQAVNKVRATKAKDDNNASAIANDMSANFDQSLPVEIDDKIHMLKQQNIIGI
                     GTHNGITTIHTTNHKYETTEPLNRYEKRLNPTYFIRIHRSYIINTKHIKEVQQWFNYT
                     YMVILTNGVKMQVGRSFMKDFKASIGLL"
     gene            complement(9006..10760)
                     /locus_tag="ACGEXS_001088"
     CDS             complement(9006..10760)
                     /locus_tag="ACGEXS_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFF8012107.1"
                     /translation="MLSLTMLLLERVGLIIILAYVLMNIPYFKNLMNRRRTWKARWQL
                     CIIFSLFALMSNLTGIVIDHQHSLSGSVYFRLDDDVSLANTRVLTIGVAGLVGGPFVG
                     LFVGVISGIFRVYMGGADAQVYLISSIFIGIIAGYFGLQAQRRKRYPSIAKSAMIGIV
                     MEMIQMLSILTFSHDKAYAVDLISLIALPMIIVNSVGTAIFMSIIISTLKQEEQMKAV
                     QTHDVLQLMNQTLPYFKEGLNRESAQQIAMIIKNLMKVSAVAITSKNEILSHVGAGSD
                     HHIPTNEILTSLSKDVLKSGKLKEVHTKEEIGCSHPNCPLRAAIVIPLEMHGSIVGTL
                     KMYFTNPNDLTFVERQLAEGLANIFSSQIELGEAETQSKLLKDAEIKSLQAQVSPHFF
                     FNSINTISALVRINSEKARELLLELSYFFRANLQGSKQHTITLDKELSQVRAYLSLEQ
                     ARYPGRFNININVEDKYRDVLVPPFLIQILVENAIKHAFTNRKQGNDIDVSVIKETAT
                     HVRIIVQDNGQGISKDKMHLLGETSVESESGTGSALENLNLRLKGLFGKSAALQFEST
                     SSGTTFWCVLPYERQEEE"
     gene            complement(11007..11681)
                     /gene="scdA"
                     /locus_tag="ACGEXS_001089"
     CDS             complement(11007..11681)
                     /gene="scdA"
                     /locus_tag="ACGEXS_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein ScdA"
                     /protein_id="HFF8012108.1"
                     /translation="MINKNDIVADVVTDYPKAADIFRSVGIDFCCGGQVSIEAAALEK
                     KNVDLNELLQRLNDVEQTNTPGSLNPKFLNVSSLIQYIQSAYHEPLREEFKNLTPYVT
                     KLSKVHGPNHPYLVELKETYDTFKNGMLEHMQKEDDVDFPKLIKYEQGEVVDDINTVI
                     DDLVSDHIATGELLVKMSELTSSYEPPIEACGTWRLVYQRLKALEVLTHEHVHLENHV
                     LFKKVS"
     gene            complement(11821..13545)
                     /gene="tarS"
                     /locus_tag="ACGEXS_001090"
     CDS             complement(11821..13545)
                     /gene="tarS"
                     /locus_tag="ACGEXS_001090"
                     /EC_number="2.4.1.355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(ribitol-phosphate)
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFF8012109.1"
                     /translation="MMKFSVIVPTYNSEKYITELLNSLAKQDFPKTEFEVVVVDDCST
                     DQTLQIVEKYRNKLNLKVSQLETNSGGPGKPRNVALKQAEGEFVLFVDSDDYINKETL
                     KDAAAFIDEHHSDVLLIKMKGVNGRGVPQSMFKETAPEVTLLNSRIIYTLSPTKIYRT
                     ALLKDNDIYFPEELKSAEDQLFTMKAYLNANRISVLSDKAYYYATKREGEHMSSAYVS
                     PEDFYEVMRLIAVEILNADLEEAHKNQILAEFLNRHFSFSRTNGFSLKVKLEDQPQWI
                     NALGDFIQAVPERVDALVMSKLRPLLHYARAKDIDNYRTVEESYRQGQYYRFDIVDGK
                     LNIQFNEGEPYFKGIDIAKPKVKMTAFKFDNHKIVTELTLNEFMIGEGHYDVRLKLHS
                     RNKKHTMYVPLSVNANKQYRFNIMLEDIKAYLPKEKIWDVFLEVQIGTEVFEVRVGNQ
                     RNKYAYTAETSALIHLNNDFYRLTPYFTKDFNNISLYFTAITLTDSISLKLKGKNKII
                     LTGLDRGYVFEEGMASVVLKDDMVMGMLSQTSENEVQILLSKDIKKRDFKNIVKLNTA
                     HITYPLNK"
     gene            complement(13578..15266)
                     /gene="tarL"
                     /locus_tag="ACGEXS_001091"
     CDS             complement(13578..15266)
                     /gene="tarL"
                     /locus_tag="ACGEXS_001091"
                     /EC_number="2.7.8.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA];
                     GO_function: GO:0047356 - CDP-ribitol
                     ribitolphosphotransferase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid ribitol-phosphate polymerase TarL"
                     /protein_id="HFF8012110.1"
                     /translation="MVKSKIYIDKIYWERVQLFVEGHSENLDLEDSNFVLRNLTETRT
                     MKANDVKIDGNQFVCRFNVAILDNGYYLPEDKYLLVNEQELDYIAQLNPDVINDAYQN
                     LKPEQEEEYNELETQNGKINFLLQTYLKEFRKGGISKKTVYTVTPEISSDVNEFVLDV
                     VVTTPEVKSIYIVRKYKELRKYFRKQSFNTRQFIFKAIFNTTKFFHLKKGNTVLFTSD
                     SRPTMSGNFEYIYNEMLRQNLDKKYDIHTVFKANITDRRGIIDKFRLPYLLGKADYIF
                     VDDFHPLIYTVRFRRSQEVIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKAYV
                     SSETDIPFYAEAFGIKEKNVVPTGVPRTDVLFDEAYATQIKQEMEDELPIIKGKKVIL
                     FAPTFRGSGHGTAHYPFFKIDFERLARYCEKNNAVVLFKMHPFVKNRLNIADKHKQYF
                     VDVSDFREVNDILFITDLLISDYSSLIYEYAVFKKPMIFYAFDLEDYITTRDFYEPYE
                     SFVPGKIVQSFDALMDALDNEDYEGEKVIPFLDKHFKYQDGRSSERLVRNLFGS"
     gene            complement(15288..16313)
                     /locus_tag="ACGEXS_001092"
     CDS             complement(15288..16313)
                     /locus_tag="ACGEXS_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribitol-5-phosphate dehydrogenase"
                     /protein_id="HFF8012111.1"
                     /translation="MINQVYQLVAPRQFEVTYNNVDIYSDYVIVRPLYMSICAADQRY
                     YTGSRDENVLSQKLPMSLIHEGVGEVVFDSKGVFNKGTKVVMVPNTPTEKDDVIAENY
                     LKSSYFRSSGHDGFMQDFVLLNHDRAVPLPDDIDLSIISYTELVTVSLHAIRRFEKKS
                     ISNKNTFGIWGDGNLGYITAILLRKLYPESKIYVFGKTDYKLSHFSFVDDVFFINKIP
                     EGLTFDHAFECVGGRGSQSAINQMIDYISPEGSIALLGVSEFPVEVNTRLVLEKGLTL
                     IGSSRSGSKDFQDVVDLYIQYPDIVDKLALLKGQEFEIATINDLTEAFEADLSTSWGK
                     TVLKWIM"
     gene            complement(16306..>16903)
                     /locus_tag="ACGEXS_001093"
     CDS             complement(16306..>16903)
                     /locus_tag="ACGEXS_001093"
                     /EC_number="2.7.7.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-ribitol-5-phosphate cytidylyltransferase"
                     /protein_id="HFF8012112.1"
                     /translation="ILINDFEKIIIATPQQWMTHTKDTLRKFKISDERIEVIQGGSDR
                     NDTIMNIVKHIESTNGINDDDVIVTHDAVRPFLTHRIIKENIQAALEYGAVDTVIDAI
                     DTIVTSKDNQTIDAIPVRNEMYQGQTPQSFNINLLKESYAQLSDEQKSILSDACKIIV
                     ETNKPVRLVKGELYNIKVTTPYDLKVANAIIRGGIADD"
BASE COUNT         5394 a         3164 c         2566 g         5779 t
ORIGIN      
        1 atttttaaat atgatttaaa cgttttcagt ttttatgaaa acgcatgcat tttacaaata
       61 aaaatggtac gatggcactg gtaaaacgtt ttactaaaaa caaatcatga ggtgtataac
      121 atgagcattg ttgcattact tatcgggtta ggccccttaa ttggctgggg cttcttccca
      181 acagtcgctt caaagtttgg tggtaaacct gtacatcaaa ttatcggtgc tactgtaggt
      241 acgttaatct tcgctattat attagccgta gtcacatcaa gtggcttccc tactggaacc
      301 aatttgctat tcgccttatt atcaggtgca ggatggggat tcggacaaat cattacattt
      361 aaagcgttcg aattagtcgg ctcatctcgt gccatgccag tcacaacagc attccaatta
      421 ttaggcgcat ctttatgggg tgtctttgca ttaggaaatt ggccaggcat tggtcataaa
      481 atcattggat ttacagcttt agtcgttatt ctaattggag cgcgtatgac agtttggagt
      541 gaacgcaaag aagcaagtaa cgccaaaaat ttacgtcgtg cagtggtact tctgttaatt
      601 ggtgaatttg gatactggtt atattcagct gcaccgcaag caacttctat tgatggccta
      661 actgcctttt tacctcaagc aatgggtatg gtaattgttg cagtcattta tggctttatg
      721 aatatgaaag cagagaatcc attccgtaat aaaattacgt ggttacaaat tatttcaggt
      781 ttcttctttg catttggtgc tttaacatat cttatttcag cacaacctaa tatgaatggt
      841 ttagcaactg gatttattct ttctcaaaca tccgttgtgc ttgctacatt aactggtatt
      901 tatttcttaa aacaacataa aacgtcaaaa gaaatggtta ttacaatcat cggcttagta
      961 ctcattttag tagccgcttc tgttacagta tttataaaat aaggagtgta gatgtcatga
     1021 aaaaatcagc tgttttaaat gaacatattt caaaagcaat cgcgacaatt ggtcattttg
     1081 atttattaac gattaatgac gctggcatgc caattccaaa tgatcatcgt cgtatcgacc
     1141 tagctgtaac taaaaactta ccacgcttta ttgatgtctt agctacagtg ttagaagaaa
     1201 tggaaatcca aaaaatatac ttagcagaag aaataaaaga acataaccct acacaattgc
     1261 aacaaattaa acaattgatt tcatcggaaa tcgaaatcat tttcattcct cacgaagaaa
     1321 tgaaaagtaa cttagctcac ccattaaata aaggtaatat tcgtactggt gaaacaacgc
     1381 cctactctaa tattgcatta gaatcgaatg ttacttttta aaagttataa cttgaaagga
     1441 gcgtacacat gaccaacaaa gttgttattt taggttcaac gaatgtcgat caatttttaa
     1501 cagttgaaag atatgcacaa ccaggcgaaa cattacatgt tgaagaagca caaaaagcat
     1561 tcggcggagg taaaggtgcc aaccaggcta ttgccactgc acgcatgcaa gcagacacaa
     1621 catttattac taaaattggc actgatggcg ttgctgattt catcttagaa gattttaaag
     1681 tagctcatat tgatacatca tatattatca aaacagctga agcaaaaacg ggccaagcct
     1741 ttatcactgt gaatgcagaa ggacaaaaca ccatctatgt ttatggtggt gcgaatatga
     1801 cgatgacacc tgaagatgtt attaacgcaa aagacgctat aatcaatgca gactttgtcg
     1861 ttgcacaatt agaagtaccc atcccggcta ttatatctgc atttgaaatt gccaaggcac
     1921 atggtgtgac gacagtatta aatcctgcac cagcgaaagc attacctaat gaattattat
     1981 cattaatcga tattattgtg ccaaacgaaa cagaagccga attgttatct gggattaaag
     2041 taactaatga acaatctatg aaagacaatg ccaattactt tttatctata ggcattaaga
     2101 ctgttttgat tacgctaggt aagcaaggta catattttgc tactaaaaat caaagccaac
     2161 acatcgaagc ttataaagta aatgcgattg atacaactgc tgcaggcgac acatttattg
     2221 gtgcatttgt cagtcgctta aacaagtcgc aagataactt agcagatgct attgattttg
     2281 gtaataaagc gagctcactc actgtacaaa aacacggcgc gcaagcatct attcctctac
     2341 tagaagaagt aaatcaagtt taaatgaatc aaacacagct atgatatgaa ggtttagcat
     2401 ataacatgca acattcgtat atcatggctg tgctttttta tctttataaa acatcatcta
     2461 ttagaaataa ttttccacta aacctatgct tgttgactca tgtttagtta taaatgaagt
     2521 gacaattttt tgtaatcttt ttaacttcca aattatcgca tataaatatg ctatattaat
     2581 gataataatt atcaattaaa aggaggttat gctatgtcta aagaagctgg tcatacattt
     2641 ttagctaaat taggaaaaac tcgtctacgc cccggtggta aagaagcaac agattggtta
     2701 atacaacaag gggcattttc acaagataaa caagtgttag aagtggcatg taatatgtgc
     2761 acaacatcta tttatctagc tcatacatat ggctgtcaca ttcaaggcgt tgatataaat
     2821 aagaaagcat tagaaaaagc acaggaaaac atttcagcag caggtcttga atcatatatt
     2881 caagttcaac aagcgaatgc tgttaaattg ccctttgatg acaatcaatt cgatatcgtt
     2941 ttaaatgaag caatgttaac aatgttaccc atcgccataa aggaaaaagc attacgcgag
     3001 tactaccgag tcttaaagcc tgggggtatc ttgttaacac atgatattgt catcgttaat
     3061 gaatcacatg ccacacatgt tgttaaatca ttatctgcag caattaatgt caatgtctca
     3121 ccgcagacga aacttggctg gttagattta tataatcaag ctggttttaa tcatgtgcat
     3181 tatcatactg gtccaatgag tttaatgaca ccaaaaggtt taatttatga cgaaggtatt
     3241 gttggaacta taaagattat caacaatgct ttgaaaaaag aaaatcgacc aatgttttgt
     3301 aaaatgttta aaacgatgac taaattgcgt aaagatatga attatattac ttttgtcgct
     3361 aaaaaagagc actaaatata atgccactaa ctgtactttg tatctatgtt tgactatcac
     3421 tttaatttct ttgtgacact aatcatctac ttaacaatat cgttatcgtt gattagtaag
     3481 tcatcaattt tggttaaaga ctttcataaa cactcaaaca ttaacactat acatagttag
     3541 tggcattatt tttttcttaa aattttaaca tcacgggatt ggaacagaaa tgatattttc
     3601 gcaaaattta tttctgtcct tccccaactt gcattacctg tagaatttct tttcgaattt
     3661 ctctgtgttg gggccccgcc aacttgcatt acctgtagaa tttcttttcg aaattctttg
     3721 tgttgggggc ccgccattat aacactgtag gtcctactat attaatttat gtctcgggct
     3781 ctaaccctaa aatacttata atataccttt ataatttatc tccatttgat gctatcactt
     3841 gttgatacca atagaacgaa tcttttttca agcgttccat cgtgccatga ccatcattat
     3901 ctcgatcaac atatattaaa ccatagcgtt tcttcatttc accggttgta aatgaaacaa
     3961 tatcaatgat tccccacggt gtataaccga ttaaatctac accatcttga tcaactgctt
     4021 ctatcgctgc tgtaatatgt gcttttaaat attcaatgcg ataatcatca tgaatatgtc
     4081 catctaccac ttcatcaact gcaccaaaac cattttccac aataaaaagt ggtaactgat
     4141 aacgatcata taacacattc aatgtatatc ttaagccatc tggatcaatc gcccaacccc
     4201 aatcactcgt tgcgatatgc ggattctcca cagaatgatt caaaccaccg ttgacgatgt
     4261 tgttttcaac tgtagtatca acatcatgtt ttacagccgt agacatgtaa taactaaagc
     4321 caatataatc aaccgtgccc tgctgtaaaa tactgtcgtc cccttcttgc catccaacat
     4381 catatccttt tcgtgccaac atttttttag catagcttgg ataataacct ctcacttgga
     4441 catccgggaa aaagaaacgt aagcgattcg caatttgtgc ttccatcata tctttcggat
     4501 gacacgaata tggataaatg ggtacatgtg aaatcattgt tccaatctta aacttcggat
     4561 taatctcttt accaagacga actgctaaag cactggctaa aagttcatga tgtgctactt
     4621 gatacaagac ttcttcagga ttatcatttt ctgtcaatgc taccccagaa ttcgtccata
     4681 aaaagatagg atttgatgtg tccatctgat tattaatttc attaaacgtc atccaatatg
     4741 taactttatc tttatatctt tcaaatacaa cacgcgcaaa atgcacaaaa taatcgacaa
     4801 cttctctatt tctaaatcca ccataatgtt tcgctaaatg aagtggcatc tcaaaatgtg
     4861 ataacgtcac aacaggttca ataccttgtg caattaattc atcaaagata cgatcataaa
     4921 aggcgagtcc ttcttcattt ggcacatctt catccccatt cggaaagata cgtgtccacg
     4981 caatcgacgt acgtaaacat ttcaatccca tttctttaaa caaggcaata tcttccttat
     5041 aacgatgata aaaatcaata ccttcatgat ttggataata gtgattggga tctatagatt
     5101 ctgtaatctg acgtgctttg ccatgtgcac cactcgtcat aacatcaatt acacttaacc
     5161 ctttaccacc tttatcatat ccaccttcaa attgatttgc ggcaagagcg ccaccccaca
     5221 tgaaattttg cggtaatttt gtcattatta caacacactc ctagttttta aatattttaa
     5281 aaataacatc ttcagtcgta atgatttcat tcattgtcag ttctactttt cctaaatcgg
     5341 cagagttgct aataacgaca ataacgtcag cattgtagcc ttgttgttgt atatattgct
     5401 ggtcgaattg caacaacgtt tgccctgctt caacatgatc attttgcttt acaaagcact
     5461 tgaacccttt accttccaag tcaactgtat tcacgccaat atgaatgact atgtccacac
     5521 cgtcttctga ttgaatacca actgcatgct tagttggtac aatcatagat attaaaccat
     5581 tgaacggtgc gattactttg gattcttcat gagctttgat tgctaagcct tcacctacca
     5641 ttttctctct aaaaattgaa tctcttactt ctttaagtaa aatacgacga ccagcgattg
     5701 gtgctttcac taaaattggt gttgttactt ttacttgagg tgtataactt gtatcttgac
     5761 tattaatatt gtcagtattc tcactctctt cgacattagc attcttaatt aatcgctgta
     5821 attgctgata cacttcatcg acatttccat ttaattttat gactaactgc gtatcatttt
     5881 ctgctacaga tgtcacatca actatttgtt tcacttcatt tgctgtagaa ggaattgtat
     5941 gttgcatatg aattgtaatg cacttcgtat catacgttgc atcaacaatg ttttcgacac
     6001 cacctacagc tgtaagaatg tcttgggctt gttctttata tttattgctc ataaatatta
     6061 ccccctcaac ttgatagtag aatgaaaatg tatttactta cctccaatta taattgtacc
     6121 ggttcaattt gtaaacgccg atacaattat aatattttgt gctataataa ttacagacaa
     6181 agtgaaacga ggacagaata ttgttaaagt atgaacatat tgctaagcaa cttaatgcgt
     6241 ttatacatca atctaatttc aaacccggtg ataaattgcc aagcgtgacg caattaaaag
     6301 aacgttatca agtaagtaag agtactatca ttaaagcatt aggcttattg gaacaagatg
     6361 gtttgatcta tcaagcacaa ggcagtggta tttatgtgag aaatattgct gatgccaatc
     6421 gtatcaacgt ctttaagact aatggtttct ctaaaagttt aggtgaacac cgaatgacaa
     6481 gtaaggtact tgtttttaag gagattgcaa cgccacctaa atctgtacaa gatgagctcc
     6541 aattaaatgc agatgatacc gtctactatt tagagcgatt aagattcgtg gacgatgatg
     6601 ttttatgtat cgaatattct tattatcata aagaaatcgt gaaatattta aatgatgata
     6661 ttgctaaggg ctctatcttc gactatttag aatcaaacat gaaacttcgt attggttttt
     6721 cagatatttt ctttaatgta gatcaactca cttcaagtga agcttcatta ctacaattgt
     6781 ctacaggtga accatgttta cgttaccacc agacttttta tacaatgact ggcaaaccct
     6841 ttgattcatc tgacatcgta tttcattatc gtcatgcaca gttttatatt cctagtaaaa
     6901 agtaataaat acataaaaac gtctatatcc cagttataaa ctggagtata gacgtttttt
     6961 tacgataata acaatggctc aaattgctat tatcttgctt aggtttttcg ttttagaaga
     7021 atattgctac aaagacaggc acaactgcta caacaactac accaactaac actaaagcta
     7081 tacttgccat tgattcttct acaggtccta attctttggc tggtgctaca cctaatgtgt
     7141 gaccacttgt tccaagtgct aatcctcggg caatagggtt agtaattcgg aaaagcttta
     7201 agaatttatt acctagggca taaataatga caccatttaa aataactgct aatgatgtta
     7261 attcttttat accaccgata ccagctgata ctggtaacgc aatcgctgta gttgctgctt
     7321 gaggtaacat tgataaaata acatcattgg caaattgtgc taacttcgca aaagttaaaa
     7381 taattaataa cgctacaact gtaccgatac caatacctcc gatgatacga tgccaatgtt
     7441 taacaagcac ttcacgcttt ttatataacg gaatcgcaaa acagattgtt gccggttcta
     7501 agaagaagta aataatgtct ccacctattt tgtaagtctt atacggaatg cctgttaaat
     7561 agaggaaggc cacaccaaat accatactga caaatagcgg tgcgaataag aagaaacgat
     7621 tagttttttc aaataatatg gtcgctaaga aaaatggtat aacggataac agtattccga
     7681 agtaaggtgt gtttagtgct aagtggttaa tcatgagctt gtgcctcctc tattttgatc
     7741 ttttttgtga ctttgtcacc tttagatctc gaagtaactt tcataataat ttgtgtgaca
     7801 tagccagtac aaataagtaa tagtattgtt gagacgatta ttagtccaat gattaaaaat
     7861 ggtgcttggc taatgacacc taaagagtta acaactgaga taccggctgg tacgaagagt
     7921 aagccaatgt tatttgttag tgtcgttcct actttttcga cttcgcctaa cttaacagca
     7981 ccagtacata ataatacaaa taataatact aaaccgatta ctgatgcagg cataggaatt
     8041 ggcataaatg attcaattat tttcgataca aagagtacta aagcaattac aatgacttgg
     8101 tgaaaaaagt gtgctggttt tgatgcgtct ttttgttgtt tcacgaccat tgcctcctac
     8161 gtttgattta actaaagtat agatggctca cttcgatttg cgtgattttt agtccgaaat
     8221 acaaaatatc ataggtaaaa tgcataaaaa aaaggattac tgttaaagta atcctatcga
     8281 cgctttaaaa tctttcataa atgaacgtcc aacttgcatc ttgacaccat ttgtcaatat
     8341 taccatataa gtatagttaa accattgttg cacttcttta atgtgtttcg tgttaataat
     8401 atatgaacga tgaatacgta taaaataagt gggattcaat cgtttttcat aacgattcaa
     8461 tggctctgtt gtttcgtatt tatgattcgt tgtatgtatg gttgtaatac cattatgtgt
     8521 gccaatccca ataatatttt gttgctttaa catgtgaatt ttatcgtcaa tttcaacagg
     8581 taagctttga tcaaaattcg ccgacatatc attcgcaatt gcacttgcgt tattatcatc
     8641 tttggcttta gtcgcacgca ctttattgac tgcttgttca atacgttttt gaccaaacgg
     8701 tttcaaaata tagtctgtcg catttaattc aaatgcctgt actgcgtatt ggtcatgtgc
     8761 agttgcaaaa ataatcgcag gtggctcttt catcttttga atcttagctc ctaattcgat
     8821 cccattttca tccattaaat tgacatctaa aaatataatg tcatattgat tgatcagtag
     8881 tgcttccaat gtttctttta cattttctgc ctcattaatt tcttcaaaac caccaatttc
     8941 atttaataaa tatgttaatt cattacgtgc taatggctca tcatctatga ttaatgcttt
     9001 catatttatt cctcctcttg tctttcataa ggaagtacac accaaaaagt ggtaccgctc
     9061 gatgtcgatt caaattgtaa tgctgcggat tttccaaata atccttttag gcgtaagttt
     9121 aaattttcta aagcactacc agttccagac tctgattcta cagatgtttc tcccaacaaa
     9181 tgcattttat ctttagaaat accctgacca ttatcttgta caataatacg tacatgtgtt
     9241 gcagtttctt taatcactga cacgtcaata tcgttacctt gctttcgatt cgtaaacgca
     9301 tgtttgatgg cattttcaac taaaatttga attaaaaatg gtggtacaag cacatcgcga
     9361 tatttgtctt caacattaat attgatatta aatcttcctg gataacgtgc ttgttcgagt
     9421 gataagtatg cacgcacttg acttaactct ttatctaaag taatcgtatg ttgcttagag
     9481 ccttgtaaat tcgctctgaa aaaataactc aattctaata gtaactctcg tgccttttcg
     9541 ctatttattc taactaaagc tgagatcgtg ttaattgaat tgaagaaaaa atgtggactc
     9601 acttgtgcct gtaatgactt aatctcagca tctttcaata acttactttg cgtttcggct
     9661 tcaccaagtt caatttggct actaaaaata tttgccaatc cttctgcaag ttgacgttcc
     9721 acaaaagtta aatcattagg gtttgtaaaa tacatcttca atgtaccgac gatagaacca
     9781 tgcatctcaa gtggtatcac gatagctgct ctaagcgggc aattcggatg actacaacca
     9841 atctcttctt tagtatgaac ttctttcaac tttcctgatt tcaatacatc tttagacaga
     9901 cttgttaata tttcatttgt tggtatgtga tgatcactac ctgcacctac atgcgataag
     9961 atttcatttt tgcttgtaat tgctacggca gatactttca ttaaattttt aataatcatc
    10021 gcaatttgct gtgccgattc tctattcaat ccttctttaa aatacggcaa tgtctggttc
    10081 atcaattgca gtacatcatg tgtttgaaca gccttcattt gctcctcttg ctttaatgtt
    10141 gaaatgataa tagacataaa aatcgccgta ccaacgctat taacaataat cattggtagt
    10201 gcaattaatg atatgaggtc aaccgcatat gctttgtcgt gggaaaatgt taaaatgctc
    10261 aacatttgaa tcatttccat aacaattcca atcatggcac ttttcgcaat actcgggtaa
    10321 cgcttgcgtc tttgagcttg taagccaaaa taaccagcaa ttataccaat aaatatagat
    10381 gagataagat aaacttgtgc atccgcccca cccatataca ctctgaaaat acctgaaata
    10441 acgccaacaa atagacctac aaaagggcca ccaactaatc ctgcgacacc tatcgttaat
    10501 acacgtgtgt tagctaaaga tacatcatca tctaaacgga agtacacact tcctgacaaa
    10561 ctatgttgat gatcgatgac gataccagtt aaattagaca ttaaggcaaa caaactgaaa
    10621 ataatacata attgccaacg tgctttccat gtacgtcgac gattcattaa gtttttaaaa
    10681 tatggaatat tcatcaacac ataggccaaa ataataatta aacctacacg ctcaagtaat
    10741 aacattgtta gcgatagcac gatagcacct cagtgaatta taattatgtt tattttatca
    10801 taatagtgca tgtaaaattc atgaataaat tattaaaaaa acacgctact atttaattat
    10861 ctataccatt aaattttcat atcaattttt atgctaagtt gaccattttt cttttcaaat
    10921 aatgctgttt atgtcactat tatgactttt atacatagcg aaaaactgct ggacttattt
    10981 ttgccaacag tttctaatcg cgttatttat gatacttttt taaataatac gtgattctct
    11041 aaatgtacgt gttcatgtgt taacacttca agtgctttta atctctgata aacaagtcgc
    11101 caagtaccac acgcttctat cggaggttca taactagatg ttaattcgct catttttact
    11161 aacaattctc ccgttgcaat gtggtctgaa actaaatcat ctatcacagt attaatatcg
    11221 tctactacct caccttgctc atatttaatg agttttggaa aatcgacatc gtcttctttt
    11281 tgcatatgct ctaacatgcc atttttaaat gtatcgtatg tttcttttaa ctcaactaaa
    11341 tatggatgat taggtccatg tactttcgat aacttcgtca cataaggtgt taaattttta
    11401 aattcttctc ttagaggttc atgatatgct gattgaatat attgaataag tgatgaaaca
    11461 tttaaaaatt taggatttaa cgaacctggt gtattcgttt gttcaacgtc gttgagacgc
    11521 tgtaataatt cgttcaaatc tacatttttc ttttctaagg ctgctgcttc tatacttact
    11581 tgtccgccac aacaaaaatc tattcccaca cttctaaaaa tatccgctgc tttcggataa
    11641 tcagttacta catctgctac tatgtcattt ttatttatca ttatagataa cctcctcatt
    11701 tccagtgtat acaatttaat gtatatgaaa atgaaaatca gggaaatgtc ataatcatgt
    11761 cataaacagc aaaataaagc cctcaaatca tgttggctca caatgatttg agggcattta
    11821 ttatttattt agtggataag tgatatgtgc agtgtttaac ttaacaatat ttttgaagtc
    11881 tcgcttttta atatctttgc taagtaagat ttgcacttcg ttttctgatg tttggcttaa
    11941 cattcccatc accatgtcgt cttttagtac gacactagcc ataccttctt caaatacata
    12001 accacgatcc agaccagtta aaatgatttt gtttttacct tttaacttca atgagattga
    12061 atccgttaat gtaatagctg taaagtataa cgaaatgtta ttaaagtctt ttgtgaaata
    12121 cggtgttaat ctataaaaat cattattcaa atgaattaat gcacttgttt ctgcagtata
    12181 tgcatattta ttacgttgat taccaacacg cacttcaaat acttccgtac ctatttggac
    12241 ttctaagaaa acatcccaaa ttttttcttt aggtaaatac gctttaatat cttctaacat
    12301 aatgttaaaa cgatattgtt tattcgcatt gacacttaaa ggtacataca ttgtgtgctt
    12361 cttgtttcgt gaatgtaatt taagtctgac atcataatgt ccttcgccaa tcataaattc
    12421 atttaacgtt agctctgtaa caattttatg attatcaaat ttaaatgctg tcattttcac
    12481 ttttggctta gcgatatcaa tgcctttaaa gtatggttcg ccttcattga attgaatgtt
    12541 taatttacca tctacaatat caaaacggta gtattgacct tgacggtaac tttcttccac
    12601 agttctatag ttgtctatat ctttcgctct cgcgtagtgc aacaatggtc gtaatttact
    12661 catcaccaat gcatctacac gttctggaac tgcttgtata aagtctccta gagcattaat
    12721 ccattgtggt tgatcttcta gtttaacttt aagtgagaag ccattcgtac gagaaaaact
    12781 aaaatgacga tttaaaaatt ctgctaagat ttgattttta tgggcttctt ctaaatctgc
    12841 atttaatatt tctacagcaa tcaatctcat gacttcataa aagtcttcag gtgaaacata
    12901 cgcactactc atatgttcac cttcacgctt tgtagcataa taatacgctt tatcacttaa
    12961 cacactgatt cgatttgcat ttaaatatgc tttcattgta aataattgat cttctgcact
    13021 ctttaattct tctggaaaat aaatgtcatt atcttttagt aatgctgttc tatagatttt
    13081 agtcgggctt aaagtataga taattcttga atttaacaaa gtaacttcag gtgctgtttc
    13141 tttaaacata gattgtggta caccacgacc attaacacct ttcattttaa tcaataagac
    13201 atctgagtga tgttcatcaa taaatgctgc tgcatccttt aaagtctctt tgtttatata
    13261 gtcatcggag tccacaaata atacaaattc accttctgct tgttttaacg ccacatttct
    13321 aggtttacct ggaccaccag aatttgtttc gagttgactt actttcaagt tcaatttatt
    13381 gcgatacttt tcaactattt gtaacgtttg atctgttgaa cagtcatcaa ctacaaccac
    13441 ttcaaattca gttttcggaa aatcttgttt cgcaaggcta ttaagtaatt ctgttatata
    13501 cttttctgaa ttgtatgttg gaactattac tgaaaatttc atcattatac ctctcccact
    13561 ttgactacta tataaactta gctaccaaat aaatttctga ctaaacgctc acttgatcgg
    13621 ccatcttgat atttaaaatg tttatctaag aatggaatga ctttttctcc ttcataatct
    13681 tcattgtcca aggcgtccat taatgcgtca aatgattgca caattttacc tggaacaaat
    13741 gattcatatg gttcataaaa atcacgcgtc gtaatataat cttctaaatc aaatgcatag
    13801 aaaatcattg gctttttaaa tactgcatat tcatatatta aagatgaata gtcactaatt
    13861 aataaatctg ttatgaacag tatatcatta acttctctaa agtcagaaac gtcaacaaaa
    13921 tattgtttat gtttgtctgc aatattaagt ctatttttca caaatggatg cattttaaat
    13981 aatacaaccg cgttattttt ttcgcaatat cttgctaaac gttcaaaatc aattttgaaa
    14041 aatgggtaat gtgctgtacc atgaccacta cctctaaatg ttggtgcgaa aagaatgact
    14101 ttcttacctt taataattgg taattcatct tccatctctt gtttgatctg tgtcgcataa
    14161 gcttcatcaa atagtacatc agtacgtgga acacctgtag gcactacatt tttctcttta
    14221 ataccaaatg cttcagcgta gaatggaata tcggtttcag atgatacata agcttttgta
    14281 tagctacgat gatttaatga atcaataaat ggtccaccct ttttaccagt acgactaaag
    14341 ccaactgttt taaaggcacc aacggcatgc catacttgaa taacttcttg agaacgtcta
    14401 aaacgcactg tataaatcaa tgggtgaaag tcatcaacaa agatgtagtc tgccttccca
    14461 agtaaatatg gcaatctaaa cttgtcgatg atgccacgtc tatctgtaat attcgcttta
    14521 aaaacagtgt gaatatcata ctttttatct aaattttgac gtaacatttc gttatagatg
    14581 tattcaaagt ttccagacat cgttggtcta gagtctgatg tgaacaacac cgtattccct
    14641 tttttcaagt ggaaaaattt cgtcgtatta aatatcgctt taaaaataaa ttgtcttgta
    14701 ttaaatgatt gtttgcggaa atacttacgt aattctttat atttacgaac gatataaata
    14761 cttttaactt ccggagtcgt tacaacaaca tcaaggacaa attcattaac atcgctagaa
    14821 atttcaggtg taacagtata aaccgttttc ttcgaaatgc cgccttttct aaattctttt
    14881 aggtaagtct gcaataagaa attgatttta ccattttgtg tttctaattc gttgtattct
    14941 tcttcttgtt ctggctttag attttgatat gcatcattaa tcacatctgg gtttaactgt
    15001 gcaatataat caagttcttg ctcattcact aataagtact tatcttcagg taagtaataa
    15061 ccattatcta agatagctac attgaaacga caaacgaatt gattcccatc tattttgaca
    15121 tcattcgcct tcattgtacg tgtctcagtt aaatttctta atacaaaatt actatcttct
    15181 aaatctaggt tttcactatg tccttcaacg aataactgaa cacgttccca atagatttta
    15241 tctatatata tcttactttt aaccaacgtt aattcatcct tttctattta cataatccat
    15301 tttaatactg ttttacccca agatgtagac aggtctgctt caaaagcttc tgtaagatca
    15361 ttaattgttg caatttcaaa ttcttgacct tttaacaacg ctaatttatc tacaatatct
    15421 gggtattgaa tgtataagtc tacaacatct tggaaatctt ttgaaccact tcgactacta
    15481 ccaatcaacg ttagtccttt ttccaatact agacgtgtat taacttctac tgggaactca
    15541 cttacaccta acagtgcaat gcttccttct ggtgaaatgt aatcgatcat ttgatttatg
    15601 gctgattgac taccgcgacc acccacacac tcaaatgcat gatcaaatgt taagccttca
    15661 ggtattttat taataaagaa gacatcatca acaaatgaga agtgactcaa tttataatct
    15721 gttttaccaa agacatatat tttagactct ggatataatt tacgtaataa aatggctgta
    15781 atgtaaccta agttaccatc accccaaata ccaaatgtat ttttatttga aatagatttc
    15841 ttttcaaaac gacgaatagc atgcaaactt actgttacaa gctctgtata tgaaataata
    15901 cttaaatcaa tatcatcagg tagtggtaca gctctatcat gatttagcaa cacaaaatct
    15961 tgcataaacc catcatgtcc acttgatctg aagtagctcg attttaaata gttttcagca
    16021 atgacatcgt ctttttctgt cggcgtattc ggtaccataa ctacttttgt acctttatta
    16081 aacacacctt tactgtcaaa tacgacctca ccaacacctt catgaattaa agacattggc
    16141 aatttctgag ataagacatt ctcatcacgg ctaccagtat aatatctttg atcggcagca
    16201 caaattgaca tatataaagg acgtacaatg acatagtcac tgtaaatatc tacgttgtta
    16261 tacgtaactt caaattgtct aggtgcaact aattgatata cttgattaat catcggcaat
    16321 accacctcga ataatagcat tcgctacttt taaatcgtaa ggtgttgtta ctttaatgtt
    16381 atataactca ccttttacaa gtcgaaccgg tttgtttgtt tctacaataa tcttacaagc
    16441 atcagataaa atactctttt gctcatcact caactgtgca tagctttctt ttaataaatt
    16501 aatattaaac gattgaggtg tttgaccttg gtacatttca ttacgcactg gaattgcatc
    16561 aatcgtttga ttatctttag atgtaacaat cgtatctata gcatcaatca ctgtatctac
    16621 tgcaccgtac tctaaagcag cttgaatatt ttctttaata atacgatgcg ttaaaaatgg
    16681 tctaactgca tcatgtgtca caatgacatc gtcatcgtta ataccatttg ttgattcaat
    16741 atgtttaacg atattcataa ttgtatcgtt acgatcgcta ccaccttgaa tgacttcaat
    16801 tctttcatca gaaattttga attttctaag tgtatctttc gtatgcgtca tccattgttg
    16861 tggcgtcgcg ataataattt tttcaaaatc attaattaaa ata
//
LOCUS       DAXWFK010000042        16768 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.042, whole genome shotgun sequence.
ACCESSION   DAXWFK010000042 DAXWFK010000000
VERSION     DAXWFK010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16768
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.042"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..275
                     /locus_tag="ACGEXS_001094"
     CDS             <1..275
                     /locus_tag="ACGEXS_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012113.1"
                     /translation="PLLFNALLYLILTIILLVNRQNAIRPIAIIPIFGIVGSFLAIIP
                     FLGILIHWILFFLMILFVLVVLSAPTYIPNKNARVIYTQYKDGHRE"
     gene            complement(467..1801)
                     /locus_tag="ACGEXS_001095"
     CDS             complement(467..1801)
                     /locus_tag="ACGEXS_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFF8012114.1"
                     /translation="MKNYFQFDKYGTNFKREILGGITTFLSMAYILAVNPQVLSLAGV
                     KGVSEDMKMDQGAIFVATALAAFVGSLFMGLIAKYPIALAPGMGLNAFFAFTVVLTMG
                     IPWQVGLTGVLFSGIFFAILTVTGFREVIINAIPYQMKMAVSAGIGLFITFVGLQSSG
                     IIVKNESTLVTLGHLTDPPVLLAIFGIVITVILYAIKLPGSIFIGMIITAIVGMFTGL
                     IQMPSGIVGKIPSIEPTFGAAFEAFKDPSQLLTIQFLIVILTFLFIDFFDTAGTLVAV
                     ATQAGIMKDNKLPRAGRALFSDSLATIVGSVFGTTTTTSYIESTSGVAVGARTGFASI
                     VTGCCFLLALFFSPLIAVVTSAVTTPALVVVGVLMAANFAEINWKSFEVAVPAFITII
                     MMPLSYSIATGIACGFIFYPITMLISKKHKEVHPIMYVLMVLFILYFIFVHG"
     gene            complement(1914..4049)
                     /locus_tag="ACGEXS_001096"
     CDS             complement(1914..4049)
                     /locus_tag="ACGEXS_001096"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFF8012115.1"
                     /translation="MKSLILAEKPSVARDIADALQINQKRNGYFENNQYIVTWALGHL
                     VTNATPEQYDKNLKEWRLEDLPIIPKYMKTVVIGKTSKQFKTVKALILDNKVKDIIIA
                     TDAGREGELVARLILDKVGNKKPIRRLWISSVTKKAIQQGFKNLKDGRQYNDLYYAAL
                     ARSEADWIVGINATRALTTKYDAQLSLGRVQTPTIQLVNTRQQEINQFKPQQYFTLSL
                     TVKGFDFQLESNQRYTNKETLEQMVNNLKNVDGKIKSVATKHKKSYPQSLYNLTDLQQ
                     DMYRRYKIGPKETLNTLQSLYERHKVVTYPRTDSNYLTTDMVDTMKERIQATMATTYK
                     DQARPLMSKTFSSKMSIFNNQKVSDHHAIIPTEVRPVMSDLSNRELKLYDMIVERFLE
                     ALMPPHEYDAITVTLEVAGHTFVLKENVTTVLGFKSIRQGESITEMQQPFSEGDEVKI
                     SKTNIREHETTPPEYFNEGSLLKAMENPQNFIQLKDKKYAQTLKQTGGIGTVATRADI
                     IDKLFNMNAIESRDGKIKVTSKGKQILELAPEELTSPLLTAQWEEKLLLIERGKYQAK
                     TFINEMKDFTKDVVNGIKNSDRKYKHDNLTTTECPTCGKFMIKVKTKNGQMLVCQDPS
                     CKTKKNVQRKTNARCPNCKKKLTLFGKGKEAVYRCVCGHSETQAHMDQRMKSKSSGKV
                     SRKEMKKYMNKNEGLDNNPFKDALKNLNL"
     gene            complement(4075..4170)
                     /locus_tag="ACGEXS_001097"
     CDS             complement(4075..4170)
                     /locus_tag="ACGEXS_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012116.1"
                     /translation="MILTCYNTFNTSFAIIEFKSKISGIDEARSK"
     gene            4236..5123
                     /locus_tag="ACGEXS_001098"
     CDS             4236..5123
                     /locus_tag="ACGEXS_001098"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8012117.1"
                     /translation="MSIVQLYDITQIKSFIEHSNYESASYLYKLPQQYNEIDVLITDA
                     IESPGVFSIKENDSIKAIILSFAYDKNKFKVIGPFVADNYVLSVDTFETLFKAMTSNQ
                     PDDAVFNFSFEEGIQQYKPLMKVIQASYNFTDYYIEARTRLEEDMHQPNIIPYHKGFY
                     RAFSKLHTTTFKYQAQSPQDIIDSLDDHHHLFLFVSEGLLKGYLYLEIDSQQSIAEIK
                     YFSSHVDYRLKGIAFELLAYALQYAFDNFDIRKVYFKIRNKNNKLIERFNGLGFHINY
                     EYIKFKFESRNVKDQTIPE"
     gene            5304..6167
                     /locus_tag="ACGEXS_001099"
     CDS             5304..6167
                     /locus_tag="ACGEXS_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFF8012118.1"
                     /translation="MQFLDFLIALLPALFWGSVVLINVFVGGGPYNQIRGTTLGALIV
                     GLGLLITGFAKFNNPTVIIVGLISGALWAFGQANQLKSISLIGVSNTMPVSTGMQLVG
                     TTLFSVIFLGEWSSMTQIIFGLIAMILLVTGVALTSLKAKNERQSDNPEFKKAMGILI
                     VSTVGYVGFVVLGDIFGVGGTDALFFQSVGMAIGGFILSMNHKTSLKSTALNLLPGVI
                     WGIGNLFMFYSQPKVGVATSFSLSQLLVIVSTLGGIFILGERKDRRQMTGIWAGIIII
                     VIAAIILGNLK"
     gene            complement(6284..6415)
                     /locus_tag="ACGEXS_001100"
     CDS             complement(6284..6415)
                     /locus_tag="ACGEXS_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012119.1"
                     /translation="MYGAYLIYEYHQDFKSIANFKVLKRMINVMMCYCVLFLEPRIS"
     gene            complement(6586..7494)
                     /locus_tag="ACGEXS_001101"
     CDS             complement(6586..7494)
                     /locus_tag="ACGEXS_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002487080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFF8012120.1"
                     /translation="MTEQFTMIILLIALGYFLKRINFIKATDSQVIATLVLNVTLPSL
                     VIVNLNSAELKLSFSILPILMIIYGIVAKMIAVWFFRKYDNHMRGSVGMMTGAMNIGL
                     FAYPLIEVIWPKTGLIYFVMADIGGAFVMFGITYFVGSYFSEGSDQFDFKYLGKKLLQ
                     SVPLVTYIVMFILNISHIHLPHVAIDFFSILSHANMPLSMILLGVMLNFTLERKYIPA
                     TIKYLCLHYGLALVAGLLVYVLLPVSDDMIKTTLLVTWMFPVGVAIISYGIQFKYRTL
                     PFIGMTTNLTIIISIIILYVYQAVFV"
     gene            7627..7791
                     /locus_tag="ACGEXS_001102"
     CDS             7627..7791
                     /locus_tag="ACGEXS_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SE1832 family protein"
                     /protein_id="HFF8012121.1"
                     /translation="MSLENQLAELKYDYVRLQGDIEKRESLNLDTSALVRQLKDIENE
                     IRNVRAQMQD"
     gene            8036..8353
                     /gene="mspA"
                     /locus_tag="ACGEXS_001103"
     CDS             8036..8353
                     /gene="mspA"
                     /locus_tag="ACGEXS_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane stabilizing protein MspA"
                     /protein_id="HFF8012122.1"
                     /translation="MQFYLILLAILYLIVSFISIFKMEVVFTRILRIIMGVLLLFVLA
                     LTTMSFPKENWWVFIVLLLLVGNVEVTGFKMLKKDLKGVNILNLMSLFIFVIYFILTI
                     VLF"
     gene            complement(8540..11707)
                     /locus_tag="ACGEXS_001104"
     CDS             complement(8540..11707)
                     /locus_tag="ACGEXS_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HFF8012123.1"
                     /translation="MIKKLLQFSLGNKFAIFLMVVLVVLGGVYASAKLKLELLPNVQN
                     PVISVTTTMPGATPQSTQDEISSKIDNQVRSLAYVKNVKTQSIQNASIVTVEYENNTD
                     MDKAEEQLKKEIDKIKFKDEVGQPELRRNSMDAFPVLAYSFSNKENDLKKVTKVLNEQ
                     LIPKLQTVDGVQNAQLNGQTNREITLKFKQNELEKYGLTADDVENYLKTATRTTPLGL
                     FQFGDKDKSIVVDGQYQSVDAFKNINIPLTLAGGQGQSQSQSDNKQNSAMSDVNQASP
                     QQNSKASAPNNISGMPTAKLGDLADITVGDVRTSISKTNGKDAVNLQITKAQDANTVQ
                     VAKDVQRKIDTFVDENKDFNVTKTMDTAKPVEKSLYTMVEKASLGTIVAIIVILLFLR
                     NIRTTAISIISIPLSLLMALIALKLSDVSLNILTLGALTVAIGRVIDDSIVVVENIYR
                     RLTDSEEQLKGENLIISATTEVFKPIMSSTLVTIIVFLPLVFVSGSVGEMFRPFALAI
                     AFSLLASLLVSITLVPALAATLFKKGVKRRNKQHQEGLGVVSTTYKKVLHWSLNHKWI
                     VIILSTLILVATIVFGGPRLGTSFISAGDDKFLAITYTPKPGETEQAVLNHAKDVEKY
                     LKQKKHVKTIQYSVGGSSPVDPTGSTNSMAIMVEYDNDTPNFDVEADKVIKHADGFKH
                     PGEWKNQDLGTGAGNKSVEVTVKGPSMDAIKSTVKDIEQKMKQVKGLANVKSDLSQTY
                     DQYEIKVDQNKAAENGISASQLAMHLNENLPEKTVTTVKENGKTVDVKVKQNKQTDWS
                     EDKLNNITLKKPTGGTIKLGDIATLVKTTTPSKLTQEQGDYATTVSAKVTNKDVGGTT
                     RQVMSKINNLDKPNNVKVNIGGASDDINNAMTQLAFTMLAAIIIVYLILVITFKGGLA
                     PFTILFSLPFTVIGVIIALLITGETISVPSLIGMLMLIGIVVTNAIVLIDRVINNEQQ
                     GMEMKEALIEAGGTRIRPILMTAIATIGALVPLLFGQDSSILISKGLAATVIGGLISS
                     TLLTLVVVPVIYEILFTLKKRFTKR"
     gene            complement(11824..13089)
                     /gene="femX"
                     /locus_tag="ACGEXS_001105"
                     /gene_synonym="fmhB"
     CDS             complement(11824..13089)
                     /gene="femX"
                     /locus_tag="ACGEXS_001105"
                     /gene_synonym="fmhB"
                     /EC_number="2.3.2.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase"
                     /protein_id="HFF8012124.1"
                     /translation="MEKMHITNQEHDAFVKSHPNGDLLQLTKWAETKKLTGWYARRIA
                     VGRDGEVQGVAQLLFKKVPKLPYTLCYISRGFVVDYSNKEALNALLDSAKEIAKAEKA
                     YAIKIDPDVEVDKGTDALQNLKALGFKHKGFKEGLSKDYIQPRMTMITPIDKNDDELL
                     NSFERRNRSKVRLALKRGTTVERSDREGLKTFAELMKITGERDGFLTRDISYFENIYD
                     ALHEDGDAELFLVKLDPKENIAKVNQELNELHAEIAKWQQKMKTSEKQAKKAQNMIND
                     AQNKIAKNEDLKRDLEALEKEHPEGIYLSGALLMFAGSKSYYLYGASSNEFRDFLPNH
                     HMQYTMMKYAREHGATTYDFGGTDNDPDKDSEHYGLWAFKKVWGTYLSEKIGEFDYVL
                     NQPLYQLIEQVKPRLTKAKIKISRKLKRK"
     gene            13478..13729
                     /locus_tag="ACGEXS_001106"
     CDS             13478..13729
                     /locus_tag="ACGEXS_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012125.1"
                     /translation="MRTIILSLFIIMNIVAIIMTLSQPLTVNYFSLRVILIFFTFILS
                     IFFILIKSSRLNNILTILSIVLVIIHMGILAHSTYVYLY"
     gene            complement(13721..14485)
                     /locus_tag="ACGEXS_001107"
     CDS             complement(13721..14485)
                     /locus_tag="ACGEXS_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFF8012126.1"
                     /translation="MILKFDHIIHYIDQLDRFSFPGDVIKLHSGGYHHKYGTFNKLGY
                     INENYIELLDVENNEKLKKMAKTIEGGVAFATQIVQEKYEQGFKNICLRTNDIEAVKN
                     KLQSEQVEVVGPIQMERDTHKDGKVKWQLLYIMNQDDDEIKPPFFIQWEESDSMRTKK
                     LQKYFQKQFSIETVIVKSKNRSQTVSNWLKWFDMDIVEENDHYTDLILKNDDIYFRIE
                     DGKVSKYHSVIIKDAQATSPYSIFIRGAIYRFEPLV"
     gene            14647..15087
                     /locus_tag="ACGEXS_001108"
     CDS             14647..15087
                     /locus_tag="ACGEXS_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFF8012127.1"
                     /translation="MLSQEFFNSFITIYRPYLKLAEPILEKHNIYYGQWLILRDIAKH
                     QPTTLIEISHRRAIEKPTARKTLKALIENDLITVENSLEDKRQKFLTLTPKGHELYEI
                     VCLDVQKLQQAVVAKTNISQDQMQETINVMNQIHEILLKEAHND"
     gene            15080..16291
                     /locus_tag="ACGEXS_001109"
     CDS             15080..16291
                     /locus_tag="ACGEXS_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8012128.1"
                     /translation="MTNQGSLNKSPIFTKSFTINFLVNFIVYLCMYLLLVVIAGYSKQ
                     AFDASDSLAGLVVGLFIVGSLIGRFATGKFVNQIGPKRLLFIGLIALIITQLLYFIDG
                     SLAFLIFVRLINGIATAVVTTATGTIAAYVTPVNRKSEGISLFSLSLVLGTAIGPFLG
                     MLLITKYAIDLLFIICVILGILGLIISLFIKVDFEVTNNKTETNVIDKPRFSIHQFIA
                     KEAIPVAVIMLLIGVTYASILTYLQAFALERHLVTAASYFFICYAIASLITRPIAGRL
                     MDDKNENIIVYPAFIMLFLSFVCLISSYQSWLILLAGACLGLGYGNLSSAMQSIAIKV
                     SPPIKYGIATSTFYVGLDAGVGFGPSFLGLFTHMFSYSEIFGFMAALAIITMLVYFLI
                     HGRHVTRNAMN"
     gene            complement(16393..16743)
                     /gene="sarV"
                     /locus_tag="ACGEXS_001110"
     CDS             complement(16393..16743)
                     /gene="sarV"
                     /locus_tag="ACGEXS_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarV"
                     /protein_id="HFF8012129.1"
                     /translation="MSNKVQRFIEAERELSQLKHWLKTTHKISIEEFVVLFKVYEAEK
                     ISGKELRDTLHFEMLWDTSKIDVIIRKIYKKELISKLRSETDERQVFYFYSTSQKKLL
                     DKITKEIEVLSVTN"
BASE COUNT         5231 a         3027 c         2420 g         6090 t
ORIGIN      
        1 agcctttact ctttaatgca ttactttatc taattcttac tattatatta ttagtgaata
       61 gacaaaatgc gattagacct atagcgatta ttccaatatt tggtatagta ggtagttttt
      121 tagctattat ccccttctta ggaattttga ttcattggat tttatttttc ctaatgattc
      181 tatttgtcct agttgtatta tcagcgccaa cctatatacc taataaaaat gctcgtgtca
      241 tttacacaca atacaaagat ggacatagag aataattctt aaaacctttt taattaggta
      301 tataatatag aagaaacacg tccaacaatt ttaaaagaag tatatttata tcggatattt
      361 aaagacattc ataatatcgc tttatgttat ttcgcttctt attttatcct tacttcaaag
      421 aaaaaaacga ctaccaatca ttggcagtcg ttttttgtgt ctggatttaa ccatgaacaa
      481 atatgaagta cagaatgaat agtaccatta atacatacat tatcggatgt acttctttat
      541 gttttttaga gataagcatt gtaattggat agaaaataaa tccacacgca ataccagttg
      601 caattgaata tgataaaggc atcataatga ttgtaatgaa ggctggcact gcaacttcaa
      661 aacttttcca gtttatctca gcaaagtttg cagccattaa tacacctacc accactaagg
      721 ctggtgtcgt aactgcactt gttacaacag cgattaacgg actaaagaac agcgctaata
      781 ggaaacaaca acctgttacg atactagcaa atcctgttct agcacctaca gcaacacctg
      841 aagttgattc gatataagaa gttgttgtag ttgttccaaa aactgatcct acaatcgtag
      901 ctaaagaatc tgagaacaat gctctaccag ctcttggtaa tttattatct ttcataatac
      961 ctgcttgagt agcaacagct actaatgtac cagctgtatc aaagaaatca ataaataaga
     1021 acgttaaaat aacaattaag aactgtatcg ttaataattg acttgggtct ttaaacgctt
     1081 caaatgcagc accgaacgtt ggttcaatac ttggaatctt acccacaata ccagatggca
     1141 tttgaatcaa tccagtgaac ataccaacga ttgctgtgat gatcataccg ataaagatag
     1201 aacccggcaa tttaattgca tataaaataa ctgtaataac gataccgaat atcgccaaaa
     1261 gtactggtgg atcagttaaa tgtcccaatg ttactaatgt agattcattt ttaactataa
     1321 tacccgaact ttgtaaacct acaaatgtaa tgaataatcc tatacctgct gaaacagcca
     1381 tcttcatttg ataaggaatt gcattaataa taacttccct aaaccctgtg acggttaata
     1441 tcgcaaagaa tatacctgag aataaaacgc ctgttaaacc aacttgccaa ggaataccca
     1501 tggttaacac aacagtaaat gcaaagaatg catttaatcc catacctggt gctaacgcaa
     1561 ttggatattt agctattagc cccatgaata gcgagcctac aaatgctgct aatgcagtcg
     1621 ctacaaaaat ggcaccttgg tccattttca tatcttctga tacgccttta acacctgcta
     1681 aacttaaaac ttgcgggtta actgctaaaa tataggccat agataagaaa gttgtgatac
     1741 cgcctaagat ttctctttta aagtttgttc catatttatc gaactggaaa tagtttttca
     1801 cgaatggatg ctcctttttt taaaatacct taatagttta atatcattct taataaaaaa
     1861 caataccaaa agcgaacttt tcgtttttct gattcaactt tgttcgattt tatctataaa
     1921 ttcaagttct ttaatgcatc tttaaacgga ttattgtcta aaccttcatt tttattcata
     1981 tactttttca tttctttacg agatacttta ccagaggatt tagacttcat acgctgatcc
     2041 atatgtgctt gcgtttcaga atgtccacaa acacaacgat ataccgcttc tttcccttta
     2101 ccaaacaacg ttaatttctt tttacagttt ggacatcttg catttgtttt gcgctgtaca
     2161 ttctttttcg tcttacaaga tggatcttgg cacacaagca tctgaccatt tttagtttta
     2221 actttaatca tgaatttacc acacgttggg cattctgtgg ttgttaaatt atcgtgttta
     2281 tatttacgat cactattttt aatcccattt acaacatctt tcgtaaaatc tttcatttca
     2341 ttaataaatg ttttcgcctg atatttacca cgttcaatta aaagtaattt ttcttcccat
     2401 tgtgcagtta aaagtggcga cgttaattct tctggtgcta attctaatat ttgtttacct
     2461 tttgacgtta ctttaatttt accgtctctt gattcaatgg cattcatatt aaataattta
     2521 tcgataatgt cggcccttgt tgcaactgtg ccgataccac ctgtttgttt taaagtttgc
     2581 gcatattttt tatccttcaa ttgaataaag ttctgagggt tctccatcgc ttttaataac
     2641 gaaccttcat taaaatattc tggaggtgtt gtttcatgtt ctctaatatt tgtttttgaa
     2701 atcttcactt catcgccttc tgaaaaaggc tgttgcatct ctgtaataga ttcaccttgt
     2761 ctaatagatt taaaacctaa aacagttgtt acattctctt tcaaaacaaa tgtgtgccct
     2821 gcaacctcta aagttacagt tatcgcgtca tactcgtgcg gaggcattaa agcttctaaa
     2881 aaacgctcga caatcatatc gtataacttt aattctctat tacttaagtc tgacatgaca
     2941 ggtctcactt ctgtaggaat aattgcatgg tgatcagata ctttttgatt attaaatatc
     3001 gacatttttg atgaaaatgt tttagacatt aatgggcgtg cttggtcttt atatgttgtt
     3061 gccatcgtcg cctgaatacg ttctttcata gtatctacca tatcagttgt taaatagttt
     3121 gaatctgttc ttggataggt tacgacttta tgtctctcat ataagctttg aagtgtattc
     3181 aatgtttctt taggtccaat tttataacgt ctatacatat cttgttgtaa atctgttaaa
     3241 ttgtacagtg attgcggata cgacttctta tgtttagtag caacagattt aatcttacca
     3301 tcgacatttt tcaaattatt aaccatctgt tctaaagttt ctttattggt atatcgctga
     3361 tttgattcta gctgaaaatc aaaccctttt accgttaatg ataatgtaaa gtattgttgt
     3421 ggtttgaact gattaatctc ttgttgtcgt gtatttacta attgaatcgt tggtgtctga
     3481 acacgtccca gggatagctg tgcatcatac tttgttgtta gtgcacgcgt tgcattaatc
     3541 ccaacaatcc aatctgcctc acttctcgct aacgctgcat aatacaaatc gttatattga
     3601 cgaccgtctt ttaaattttt aaaaccttgt tgaatagctt ttttagtaac tgagctaatc
     3661 cataatcgac ggattggctt tttgttacca actttatcca aaatcaatct tgcaactagt
     3721 tcaccttctc gtccagcatc tgttgcaata ataatatctt tcactttatt atctaaaatt
     3781 aacgctttta ctgttttaaa ttgtttgctt gttttaccaa taacaacagt tttcatatat
     3841 ttaggtataa ttggaaggtc ttctaatcgc cattccttta aatttttatc gtattgttca
     3901 ggtgtcgcat ttgtcactag atgacctaac gcccacgtga caatatattg gttattttca
     3961 aagtaaccat tacgcttctg atttatttgt aaagcatcag caatatctct tgcgactgat
     4021 ggtttttcag ctaatattaa agatttcata aattatcctt tctcatacgt tcttttattt
     4081 cgaacgtgct tcatctattc cactaatctt tgatttaaat tcaatgattg caaatgatgt
     4141 gttaaatgta ttgtaacatg ttaatatcac tattaacttt catttcagtt gaaatactat
     4201 ataataaaag taacaaaaag tacggaggta atgacatgag catagttcag ttatatgata
     4261 ttacacaaat aaaatcgttc attgaacatt cgaattatga atcagcatca tacttatata
     4321 aacttcctca acagtacaat gaaatagatg tattaataac cgatgcgatt gaatcacctg
     4381 gtgtattttc gattaaagaa aacgattcaa tcaaagcaat catattgtct tttgcatacg
     4441 ataaaaataa attcaaagtc ataggccctt tcgtggctga caattatgta ttatctgtcg
     4501 atacgtttga aacgctattt aaagcaatga cttcgaacca acctgacgat gccgtcttta
     4561 acttttcttt tgaagaaggc attcaacaat acaaaccatt aatgaaagtt attcaagcaa
     4621 gttataactt cactgactat tacatagaag cccgtacaag attagaagaa gatatgcacc
     4681 aaccaaatat cattccttat cacaaagggt tttatcgtgc tttcagcaaa ttacacacaa
     4741 ctacatttaa atatcaggca cagtcaccac aagatatcat tgatagttta gacgaccatc
     4801 atcatttgtt tttatttgtt agcgaaggtt tacttaaagg ttatttatac cttgaaattg
     4861 attcacaaca gtcaatcgcc gagattaaat acttcagttc tcatgtagat taccgtttga
     4921 aaggtatcgc tttcgagttg cttgcgtatg cattgcaata tgcttttgat aattttgata
     4981 ttagaaaagt ttattttaaa attcgtaata aaaataataa actcatcgaa cgatttaatg
     5041 gtctaggttt ccatatcaac tatgagtaca ttaaattcaa attcgaatca cgtaacgtaa
     5101 aagatcaaac aatccctgaa taaaacacca agcaaatacc ctacagtaca tcattagcat
     5161 gtattgtggg tttttctact ttttgtaaat attgaaaatt ataagtagtt gttttttact
     5221 attagggcag aatgctttac aataacatgc aagtgtcaat taaggggagc acttgcataa
     5281 atagtatagg agagtgagta gtcttgcaat ttcttgattt cttaatcgca cttttacctg
     5341 ctttattctg gggaagtgtc gttcttatta atgtgttcgt cggcggtgga ccttacaacc
     5401 aaattcgtgg tacgacgcta ggcgcactca ttgttggatt aggtttactt attactggtt
     5461 tcgcaaaatt caataatcct actgtaatta ttgtcggtct tatttctggt gcattatggg
     5521 cgtttggaca agcgaatcag cttaaatcta ttagtttaat cggtgtatca aatactatgc
     5581 cagtttctac aggtatgcaa ttagttggta caacattatt cagcgttatc tttttaggtg
     5641 aatggtcttc aatgactcaa attatctttg gtttaatcgc catgatatta ttagttactg
     5701 gtgtagcact tacttcactt aaagctaaaa atgaacgtca atcagataat cctgaattta
     5761 aaaaagcaat gggtatttta attgtatcta cagttggata tgtaggtttc gttgtacttg
     5821 gtgacatctt tggtgttggt ggaactgatg cattgttctt ccaatctgtc ggtatggcaa
     5881 ttggtggctt tatcctatcc atgaatcata aaacatcact taaatcaaca gcacttaatc
     5941 tattgccagg tgtgatttgg ggaattggta acttgttcat gttctattct caaccaaaag
     6001 ttggtgtagc tacaagtttc tcattatcac agttacttgt tatcgtttca accttaggcg
     6061 gtattttcat tttaggagaa agaaaagatc gtcgtcagat gacgggtatt tgggcaggta
     6121 ttattattat cgtgatagct gctataattc taggtaattt gaaatagaaa gttaaatact
     6181 catgtaacgt aaaaatgtaa tcacttctga aaataaccat tcacttatag aatgattaaa
     6241 attaattttc gggaatttta cgttgaatgt tcctctatat gtcctaggaa atacgtggct
     6301 ctaaaaacaa aacgcaataa cacatcatga cattaatcat gcgttttaag actttaaaat
     6361 tagcgatact tttaaaatct tgatgatatt catatatcaa gtatgcgcca tacatatgaa
     6421 gtggatagct gcataacgca ctgcattatc aacttgaatg tatgagttga acaactatgt
     6481 cataaataaa agcccccttt tcacaatata catttacata ttgtggtaaa gggggctctc
     6541 attttctacg aatactaaaa tggattttat tttcaaatgt gtaaactaga caaacactgc
     6601 ctgatacacg tacaaaataa tgatactaat aatgattgtc aaattggtcg tcatacctat
     6661 aaatggcagt gttcgatatt taaactgaat accataagaa ataattgcaa cacctaccgg
     6721 gaacatccaa gtgaccaaca atgtcgtctt aatcatatca tctgatactg gtaacaacac
     6781 atatactaac aatcccgcaa ctaatgctaa tccataatgc aaacataaat atttaatagt
     6841 agcaggtata tactttcttt ccagagtaaa attcaacatg acacctagca aaatcattga
     6901 taacggcata tttgcatggg aaagtatgct aaagaaatcg attgccacat gtggtaaatg
     6961 gatgtgactt atattcaata taaacattac aatgtatgta acgagtggca ctgattgtaa
     7021 taatttctta cctaaatatt taaaatcgaa ttgatcacta ccttcactaa agtagctacc
     7081 tacaaagtaa gtaattccaa acatcacaaa ggcaccacct atatcagcca taacaaaata
     7141 aataagtccc gttttaggcc atatcacttc aattagtgga tatgcaaaca atccaatatt
     7201 catagcacca gtcatcatac ctactgatcc acgcatgtga ttatcatatt ttctaaaaaa
     7261 ccagacagca atcattttcg ccacaatacc atatataatc attaaaattg gtaaaatgga
     7321 gaatgacaat tttaattctg cactgtttaa attcacaata actaaagatg ggagtgtgac
     7381 attaagaact aatgtagcaa tgacttgact atctgttgct tttataaaat taatgcgctt
     7441 caaaaagtaa ccaagcgcaa ttaataaaat aatcatagta aattgttctg tcactgttat
     7501 cccttctttc aataatcttc ataatttata actttaacat actccacaga tattttagaa
     7561 gtctactgtt tcatgctata atctacatta aatgcactta attatatttc aaaggagtgt
     7621 tatagtatgt ctttagaaaa ccaactagcc gaacttaaat atgattatgt tcgtcttcaa
     7681 ggtgacatag aaaaacggga atctttgaat ttagatactt ccgcacttgt tcgtcaactt
     7741 aaagatattg aaaatgaaat tagaaacgtt cgtgctcaaa tgcaagatta ataatctatc
     7801 attcaagcaa taaatgcttt ttgttacata aatttgacta gcattgctct gaatacgtta
     7861 tattgatgaa ttgcttcatt tttcgctcaa ttacatctag aatcacaaga tgttgtcgtg
     7921 ttatgattta gtgtttcatt aacaacatac acgcatatct atcccaacac tgctatttat
     7981 gttttctacg ctgctgtact acatgaaccc tttgaaacgg agaggaagtt atcatatgca
     8041 attttatctg attttactag caatacttta tctaattgtt agttttatta gtatttttaa
     8101 aatggaagtt gtatttactc gcattttgag aattattatg ggtgtgttgt tattattcgt
     8161 cttagcatta acgacgatga gttttccaaa agagaattgg tgggtattta tcgtcttatt
     8221 actcttagtc ggtaatgtcg aagtgacagg atttaaaatg cttaaaaaag atctaaaagg
     8281 cgtaaacatc ttgaatttaa tgtcattatt tatctttgtc atatatttca tcttaaccat
     8341 cgtattattc taagtaataa acaaaatatg caataacata attgcaacct tattttatta
     8401 tcggattaca tgttgaatgc cagtattgtt ttattaacac atcgattaaa tcgcacgtga
     8461 aataaggtct aaaatttcaa attttaaaaa gccataaaag tagtcaatat catgaccgct
     8521 tttatggctt ttatattatc tatcgtttag tgaatcgttt ttttaaagta aagagaattt
     8581 catatattac tggtacaact acaagtgtta ataaagttga tgaaattaaa ccaccaataa
     8641 ctgtagcagc taatcctttc gaaataagaa tcgagctatc ttgaccaaat aacaaaggaa
     8701 ctaatgcacc aattgttgca atcgccgtca ttaatatcgg tctaattcta gtaccgcctg
     8761 cttcgattaa tgcttctttc atctccatgc cctgttgctc attattaata acacggtcta
     8821 ttaacacaat ggcatttgtt actacgattc caattaacat tagcatacca attaaacttg
     8881 gtactgatat tgtttctcct gtgattaata gtgcaataat tacaccgata actgtaaatg
     8941 gtaaagagaa taaaattgta aatggtgcta ggccaccttt aaatgtaata actaggatta
     9001 aatatacgat aatgattgca gctaacattg taaaggctaa ttgtgtcatt gcattgttaa
     9061 tatcatctga tgcaccaccg atattaacct ttacattatt cggtttgtcc aaattattta
     9121 ttttagacat cacttgtcgt gttgtgccac ccacatcttt atttgttact ttagcagata
     9181 ccgtcgttgc ataatctcct tgttcttgcg tcaatttact tggtgtcgtt gttttaacta
     9241 acgtagcgat atctcccaat ttaatcgtac caccagtcgg ctttttcaaa gttatattat
     9301 tcaacttatc ttctgaccag tctgtttgct tattttgttt gactttaaca tcaacagttt
     9361 taccattttc tttaacagtc gtaactgttt tttctggtaa gttttcattc aagtgcattg
     9421 caagttgact tgcagaaata ccattttctg ccgctttatt ttgatcgact ttaatttcat
     9481 actgatcata tgtttgcgat aaatcagatt tgacattggc tagtccttta acctgtttca
     9541 ttttctgttc aatatctttt acagttgatt ttatggcatc cattgatgga ccttttacag
     9601 taacctctac agatttatta cctgcacctg ttcctaaatc ttgatttttc cactctccag
     9661 gatgtttaaa gccatctgca tgtttaataa ccttatccgc ttctacatca aaattaggcg
     9721 tgtcattatc atattcaacc atgattgcca tactatttgt actacccgtt ggatctactg
     9781 gactactacc gccaactgag tattgaattg tttttacatg ctttttctgt tttaaatatt
     9841 tttcaacatc tttcgcatga ttcaacactg cttgctccgt ttcaccaggc ttcggtgtat
     9901 aagtaatagc taaaaattta tcgtcacctg ctgaaataaa gctagtgcct agtctcggtc
     9961 ctccaaatac aatagttgca accaaaatta atgtacttaa tataattaca atccacttat
    10021 gatttaatga ccaatgtaat acttttttat aagttgtact aacaacacct aatccttctt
    10081 gatgttgttt attacgacgt ttaacgcctt ttttaaatag tgtagctgcc aacgctggaa
    10141 cgagtgtaat tgacactaat aacgatgcta ataaactaaa tgcaatagcc aatgcaaaag
    10201 gtctaaacat ttcgcctact gaacctgata caaacacaag tggtaagaag acgataatag
    10261 taactagtgt cgatgacatt attggtttaa atacttcagt tgtcgcactg ataattaaat
    10321 tttcaccttt tagttgttct tctgaatctg ttaagcgtcg ataaatattt tcaacaacta
    10381 caatcgaatc gtctatcaca cgtccaatcg ctactgttaa tgcacctaac gttagtatat
    10441 tcaatgaaac atcactcaat ttcagagcaa taagcgccat aagaagtgat aacggaatcg
    10501 atataataga aattgccgtc gtacgaatgt ttcttaaaaa cagcaaaata actataattg
    10561 ccacgattgt acctaatgat gctttttcaa ccatcgtata aagtgatttc tcaacaggct
    10621 ttgcagtatc cattgttttt gtgacattaa aatctttatt ttcatcaacg aatgtatcaa
    10681 ttttacgttg tacatctttg gctacttgaa ctgtattggc atcttgagct ttagttattt
    10741 gtagattaac cgcatccttt ccattcgttt tagaaataga agtacgcaca tcaccaactg
    10801 taatatcagc taaatctcct agtttcgctg tcggcatacc acttatatta tttggtgctg
    10861 acgcttttga attttgctgt ggtgatgcct gattaacgtc tgacatggct gaattttgtt
    10921 tattgtcact ttgggattga gattgccctt gtcctcctgc caacgttaat ggaatattta
    10981 tgtttttaaa agcatcaaca gattgatatt gaccatcaac aacaattgat ttatctttat
    11041 caccaaattg gaacaatcca agtggcgttg ttcttgttgc cgtttttaga tagttttcta
    11101 catcatcagc agtcaaccca tatttttcaa gttcattttg cttaaattta agggtgattt
    11161 cacggttcgt ctgcccattt aattgcgcat tttgcacacc atctaccgtt tgcaattttg
    11221 gtattaattg ttcattcagt actttcgtta cttttttcaa gtcattctct ttatttgaaa
    11281 atgaatatgc taaaaccgga aaagcatcca tcgaattacg tcttaattct ggttgaccaa
    11341 cttcatcttt aaatttaatt ttatcgattt cttttttaag ctgttcttct gctttatcca
    11401 tatctgtatt attttcatat tcaactgtta caattgaagc attttgtatg gattgcgttt
    11461 taacattttt cacatatgcc aatgatctta cttgattgtc aattttacta cttatttcat
    11521 cttgggtact ttgtggcgtt gcacccggca ttgttgttgt aactgaaata actggatttt
    11581 gtacatttgg tagtaattct aatttcaatt tagcactcgc atatacaccg cccaagacaa
    11641 ctaaaacaac cattaaaaag atagcaaact tattccctaa agaaaattgt aatagctttt
    11701 ttatcacagt acttccccct ctttcactat attcaaaaat attttactca accattctat
    11761 attgtgtaaa aaaatctgaa cgcaaatgac agtcttatga gcgttcagat ttcagtcgtt
    11821 aatctatttt cgttttaatt tacgagatat tttaatttta gcttttgtta aacgcggttt
    11881 aacttgctca attaattggt acaatggctg attcaataca taatcaaatt caccaatctt
    11941 ttcacttaag tatgttcccc acactttttt aaatgcccat aatccataat gttctgagtc
    12001 tttatctgga tcattatctg taccaccgaa atcgtaagtt gttgcaccat gttcacgtgc
    12061 atacttcatc atcgtatact gcatatgatg atttggtaaa aaatctctaa attcattaga
    12121 agacgcacca tataagtaat atgattttga gccagcaaac attaatagtg caccagaaag
    12181 ataaatacct tcaggatgtt ccttttctaa agcttctagg tctcgtttta aatcttcatt
    12241 tttagcaatt ttattttgcg catcattaat catattttgc gcttttttag cttgcttttc
    12301 agatgttttc atcttctgct gccatttagc aatttcggca tgaagttcat tcaattcttg
    12361 atttactttc gctatatttt cttttggatc caactttact aaaaatagtt cagcatctcc
    12421 atcttcatgc aacgcatcat aaatattttc aaagtaacta atatcacgcg ttaagaagcc
    12481 atcgcgttcc ccagtgattt tcattaactc agcaaatgtt tttaaacctt ctctatcaga
    12541 tcgttctact gtcgtacctc gctttaaagc caagcgcact tttgaacgat ttcggcgttc
    12601 aaaactattt aataactcat catcattttt atcaattggt gtaatcatag tcatacgtgg
    12661 ttggatgtag tcttttgata aaccttcttt aaatccttta tgtttaaaac caagcgcttt
    12721 caaattttgc aaagcatctg tacctttatc aacttcaaca tcaggatcga ttttaattgc
    12781 atacgctttc tcagctttag caatttcttt tgcactgtct aacaatgcat ttaacgcttc
    12841 tttattacta taatcaacaa caaaaccacg cgaaatataa catagcgtat aaggtaattt
    12901 aggtactttt ttaaaaagta actgcgcaac accctgaact tcaccgtcac gacctacagc
    12961 gattcttcgc gcgtaccatc cagttaattt ctttgtttct gcccatttcg ttaattgtaa
    13021 taaatctcca tttgggtggg atttaacaaa tgcgtcatgt tcctgattag tgatatgcat
    13081 cttttccatg atttatgata tctccttcta tttaacaata cctttaatta tacagtttgt
    13141 atcttatagt gtcgattcag agcttgtgta agatttgaac tcttattttt gaaatgtcca
    13201 tgctccaatt aatagtttag caagttcaaa tttacccatt ttaattgtga atcattttat
    13261 atctatgttt cgtgttaaat ttaatgttat cgtacaatta atacttttca actagttacc
    13321 tatacttcaa tatactttca tcatctaaca cgatattcat ttctaaaatg aaccaacttg
    13381 acttcaatga ataaattttt cctcaagcaa ccacattaat gttcatatac aattacccct
    13441 gttataatgt caataatcta acaatgaggt gtttgatatg agaacaatta ttttaagtct
    13501 atttataatt atgaacatcg ttgcaatcat tatgacattg agtcaacctc tcaccgtgaa
    13561 ttactttagt ttacgggtta tacttatctt tttcacattt atattatcaa tctttttcat
    13621 tttaattaag tcatcacgat taaataatat attaacgatt ctttccattg tgcttgtcat
    13681 tattcatatg ggcattctcg ctcatagcac ttacgtatat ttatactaat ggttcaaagc
    13741 gataaatagc acctctgata aaaattgaat atggtgaagt tgcttgtgcg tcttttatga
    13801 taaccgaatg atattttgaa actttaccat cttcaattct aaaataaata tcatcatttt
    13861 ttaaaatcaa atctgtgtaa tggtcattct cttctacaat gtccatatca aaccatttca
    13921 accaattcga tactgtttgt gatcggtttt tacttttcac aataacagtt tcaattgaaa
    13981 attgtttttg aaaatatttt tgcaattttt tagtacgcat ggaatcactt tcttcccatt
    14041 gaataaaaaa tggtggctta atttcatcat catcctgatt cattatataa agcaattgcc
    14101 actttacctt accatcttta tgtgtatctc tttccatttg aatcggccct actacttcaa
    14161 cctgctcact ttgtagttta tttttaactg cctctatatc atttgtacgc aaacaaatat
    14221 ttttaaagcc ttgctcatac ttctcttgaa caatttgagt agcaaaagcg actccgcctt
    14281 ctatcgtttt tgccatcttt ttcaactttt cattattttc tacatctagt agctcaatat
    14341 aattttcatt gatataacct aatttattga atgttccata tttatgatga tacccacctg
    14401 aatgtaattt tataacatct cctggaaaac taaaccgatc taactgatct atataatgaa
    14461 tgatgtgatc aaatttcaat atcattagta tccccctatt tacatgtaat tacgcttatt
    14521 ttaacaaagt ataattattt ttgctcttaa taattatata ttgatttcta attgctcccg
    14581 ttttataatt actattgttg taaaaaggtt agctaagcta actattttgt cttaggagat
    14641 gtcactatgc tatcacaaga atttttcaat agttttataa caatatatcg cccctattta
    14701 aaattagccg agccgatttt agaaaaacac aatatatatt atggccaatg gttaatctta
    14761 cgcgatatcg ctaaacatca gcccactact ctcattgaaa tttcacatag acgtgcaatt
    14821 gaaaagccta ctgcaagaaa aactttaaaa gctctaatag aaaatgacct tataacagta
    14881 gaaaacagtt tagaggataa acgacaaaag tttttaactt taacacctaa agggcatgaa
    14941 ttatatgaga ttgtttgtct tgatgtacaa aagctccaac aagcagtagt tgccaaaaca
    15001 aacatttcgc aagatcaaat gcaagaaacc ataaatgtga tgaatcaaat tcatgaaata
    15061 ttattaaagg aggcacacaa tgactaatca aggctcatta aataaatcac ctattttcac
    15121 caaaagtttt acaattaact ttttagtcaa ctttatcgta tacctatgca tgtatttgtt
    15181 acttgtagtt attgctggct atagtaagca agctttcgac gcttccgatt cattagccgg
    15241 attagttgta ggactattta ttgtcgggtc tttaattggt cgatttgcta caggtaaatt
    15301 tgtaaatcaa atcggaccta aacgattatt atttattggt ttaattgctt taattatcac
    15361 gcaattactt tattttattg atggatcttt agctttttta atctttgtac gtctcataaa
    15421 cggtatcgca actgcagttg tgacaactgc aacaggtact attgcggcat acgttacccc
    15481 tgttaataga aaaagtgaag gcatcagttt attttcctta agtttagtat taggtacagc
    15541 gattggccca tttctaggca tgttacttat tacaaaatac gcaattgact tactgtttat
    15601 tatttgtgtc atattaggca tactcggact cattatctca ttatttataa aagttgattt
    15661 cgaagtaaca aataacaaaa ctgaaactaa tgtcattgat aaaccaagat ttagtattca
    15721 tcaatttatt gcaaaagagg ccattcctgt tgctgtaatt atgcttttaa ttggcgttac
    15781 ctatgcgtcc atcctaactt atttacaagc atttgcttta gagcgacacc tagtcactgc
    15841 agcaagctat ttctttattt gctatgctat agcatcgtta attactagac ctattgctgg
    15901 tagattgatg gatgataaaa atgaaaatat tattgtctac ccagcattta tcatgctttt
    15961 cctttcattt gtatgcttaa tttcaagcta tcaaagctgg ttaatattac ttgctggtgc
    16021 ttgcctaggt ttagggtatg gaaatttatc atctgcaatg caatctattg cgataaaagt
    16081 ctcacccccg attaaatatg gtatcgcgac atctactttt tacgttggtc tagatgcagg
    16141 tgtcggcttc ggtccatcat tccttgggtt atttactcat atgttttcat atagtgaaat
    16201 cttcggcttt atggccgctt tagcaatcat tactatgctt gtttacttct taattcatgg
    16261 acgtcatgta actagaaatg ccatgaacta aatatttata ttcaaaaata aaaagcatgc
    16321 caatctctat tcatcatttg tctgtcccca agacgcaatt atgatgtact gattggcatg
    16381 ctttttaagt ttttagtttg taacgcttaa cacttctatt tctttagtaa ttttatctaa
    16441 caatttcttt tgagaagtac tatagaaata gaatacttgt ctttcatccg tttcagaacg
    16501 caatttagaa ataagctctt ttttatagat tttacggata atcacatcga ttttacttgt
    16561 atcccatagc atttcaaaat gtaatgtatc cctcaattct ttaccgctaa tcttttcagc
    16621 ttcatacact ttaaaaagga ctacaaattc ttcaattgaa atcttatgtg ttgtttttaa
    16681 ccagtgcttt aactgactta actccctttc tgcttctata aaacgttgaa ctttattact
    16741 cataatatga tgcctcctat gtttcttc
//
LOCUS       DAXWFK010000043        16699 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.043, whole genome shotgun sequence.
ACCESSION   DAXWFK010000043 DAXWFK010000000
VERSION     DAXWFK010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16699
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.043"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            150..695
                     /locus_tag="ACGEXS_001111"
     CDS             150..695
                     /locus_tag="ACGEXS_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFF8012130.1"
                     /translation="MQQNKRLITISMLSAIAFVLTFIKFPIPFLPPYLTLDFSDVPSL
                     LATFTFGPVAGIVVALVKNLLNYLFSMGDPVGPFANFLAGASFLLTAYAIYKNKRSTK
                     SLITGLIIATIVMTIVLSILNYFVLLPLYGMIFNLADIANNLKVIIVSGIIPFNIIKG
                     IVISIVFILLYRRLANFLKRI"
     gene            complement(801..1049)
                     /locus_tag="ACGEXS_001112"
     CDS             complement(801..1049)
                     /locus_tag="ACGEXS_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002449743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFF8012131.1"
                     /translation="MAKYTIVDMDTCIACGACGAAAPDIYDYDDEGIAFVILDDNQGT
                     AEVPEELYEDMEDAIDGCPTDSIKIADESFDGDALKFE"
     gene            1157..2110
                     /locus_tag="ACGEXS_001113"
     CDS             1157..2110
                     /locus_tag="ACGEXS_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8012132.1"
                     /translation="MQHIIKTALQQTFNYKTNKSIYNILVGKKSHQTFFDACSQQQLS
                     LYHSLPLLKYPSFELFLEKINEFNAEMEIMLHPRYTFESMGQTFQAIQLLVQTMSNTK
                     QHVFHFVPISQNNKIQETVKIVYNYIKENQLQIDFENELHNLFKAITLKGPCYLHYYL
                     QGYDEPMYTRQQVSLIEKLSQQQLFEYEMNNLVTMMFELESGEYTILSKIIMKPTLLN
                     QTYITYTKLLEQFTMEDIAAQQQVKINTIEDHVLEILIKGYMSNYDDYVELEDQLQFL
                     NFYQQHRGERLKFYKEQFDTLSYFQLKVLIVGFERGDLNVA"
     gene            2100..3479
                     /locus_tag="ACGEXS_001114"
     CDS             2100..3479
                     /locus_tag="ACGEXS_001114"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFF8012133.1"
                     /translation="MLHDILRNKFGFESFKPGQQEIIESIMSQQHTLGILPTGSGKSL
                     CYQIPTYLSGKPTLIISPLISLMDDQVMQLKINGEKRVTCIHSGMDEIEKKHNIKCLR
                     HSRFIFLSPEFLLQPSNFKLISMIDFGMIVLDEAHCLSEWGYDFRPHYALIGKVTKHF
                     KEAVVLALTATAPPHLQDDLTEMLAIQFNVIKTTMNRPNISFKHLNFHDDEDKIEWLL
                     PFLQQSGPTIIYVSSKKMCLNLAQLIYDSGFLTGIYHGDMNYQERHTVQQQFLNNDIP
                     IIVATSAFGMGINKKDIRTIIHFHLSTSPSNYIQEIGRAGRDGELSQAISLFQPDDKY
                     ILETLLFADMITEEDVQNFEIGEFLAPDKQAVLTTLQSFYSIGALKQIFKQSFKRKQL
                     GFFRMIGYCKLDQCRRKYLLEFFGEYPPAQDRCCDNDSNITDIAILNKKKVIRSIGFD
                     EKLQNLFLR"
     gene            3632..5092
                     /gene="ebpS"
                     /locus_tag="ACGEXS_001115"
     CDS             3632..5092
                     /gene="ebpS"
                     /locus_tag="ACGEXS_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elastin-binding protein EbpS"
                     /protein_id="HFF8012134.1"
                     /translation="MSNNFKDDFEKNRQSIDTNSHQDHTEDVEKDQSELEHQDTIENT
                     EQQFPPRNAQRRKRRRDLATNHNKQVHNESQTSEDNVQNEAGTIDDRQVESSHSTESQ
                     EPSHQDSTPQHEEEYYNKNAFAMDKSHPEPIEDNDKHDTIKNAENNTEHSTVSDKSEA
                     EQSQQPKPYFTTGANQSETSKNEHDNDSVKQDQDEPKEHHNGKKAAAIGAGTAGVAGA
                     AGAMAASKAKKHSNDAQNKSNSGKANNSTEDKASQDKSKDHHNGKKGAAIGAGTAGLA
                     GGAASKSASAASKPHASNNASQNHDEHDNHDRDKERKKGGMAKVLLPLIAAVLIIGAL
                     AIFGGMALNNHNNGTKENKIANTNKNNADESKDKDTSKDASKDKSKSTDSDKSKEDQD
                     KATKDESDNDQNNANQANNQAQNNQNQQQANQNQQQQQQRQGGGQRHTVNGQENLYRI
                     AIQYYGSGSPENVEKIRRANGLSGNNIRNGQQIVIP"
     gene            5217..5333
                     /locus_tag="ACGEXS_001116"
     CDS             5217..5333
                     /locus_tag="ACGEXS_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012135.1"
                     /translation="MLFNKVLSRILAYQFNFLNSHQKDKTPQSTNLTRKNKK"
     gene            5494..6480
                     /gene="ypdA"
                     /locus_tag="ACGEXS_001117"
     CDS             5494..6480
                     /gene="ypdA"
                     /locus_tag="ACGEXS_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol disulfide reductase YpdA"
                     /protein_id="HFF8012136.1"
                     /translation="MQKVESIIIGGGPCGLSAAIEQKRKGIDTLIIEKGNVVESIYNY
                     PTHQTFFSSSDKLSIGDVPFIVEESKPRRNQALVYYREVVKHHQLKVNAFEEVLTVKK
                     MNNKFTITTTKDVYECRFLTIATGYYGQHNTLEVEGADLPKVFHYFKEAHPYFDQDVV
                     IIGGKNSAIDAALELEKAGANVTVLYRGGDYSPSIKPWILPNFTALVNHEKIDMEFNA
                     NVTQITEDTVTYEVNGESKTIHNDYVFAMIGYHPDYEFLKSVGIQINTNEFGTAPMYN
                     KETYETNIENCYIAGVIAAGNDANTIFIENGKFHGGIIAQSMLAKKQTPLES"
     gene            complement(6595..7563)
                     /locus_tag="ACGEXS_001118"
     CDS             complement(6595..7563)
                     /locus_tag="ACGEXS_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFF8012137.1"
                     /translation="MKHLLVIHTGGTISMSQDQSNKVVTNDINPISMHQDVINQYAQI
                     DELNPFNVPSPHMTIQHVKQLKDIILEAVTNKYYDGFVITHGTDTLEETAFLLDLILG
                     IEQPVVITGAMRSSNEIGSDGLYNYISAIRVASDEKARHKGVMVVFNDEIHTARNVTK
                     THTSNTNTFQSPNHGPLGVLTKDRVQFHHMPYRQQALENVNDKLNVPLVKAYMGMPGD
                     IFSFYSREGIDGMVIEALGQGNIPPSALEGIQQLVSLNIPIVLVSRSFNGIVSPTYAY
                     DGGGYQLAQQGFIFSNGLNGPKARLKLLVALSNNLDKAEIKSYFEL"
     gene            7640..8299
                     /gene="cmk"
                     /locus_tag="ACGEXS_001119"
     CDS             7640..8299
                     /gene="cmk"
                     /locus_tag="ACGEXS_001119"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFF8012138.1"
                     /translation="MKAINIALDGPAAAGKSTIAKRVASELSMIYVDTGAMYRALTYK
                     YLKLNKTEDFAKLVDQTTLDLTYKADKGQCVILDNEDVTDFLRNNDVTQHVSYVASKE
                     PVRSFAVKKQKELAAEKGIVMDGRDIGTVVLPDADLKVYMIASVEERAERRYKDNQLR
                     GIESNFEDLKRDIEARDQYDMNREISPLRKADDAVTLDTTGKSIEEVTDEILAMVSQI
                     K"
     gene            8330..8446
                     /locus_tag="ACGEXS_001120"
     CDS             8330..8446
                     /locus_tag="ACGEXS_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012139.1"
                     /translation="MQRLTHIQNECADNDLLKLKGDFYSMMNESCLFIFGKS"
     gene            8563..8754
                     /locus_tag="ACGEXS_001121"
     CDS             8563..8754
                     /locus_tag="ACGEXS_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012140.1"
                     /translation="MSVTNLNIAKEADLVTKNVLCNNSRKMHYIRSSYYSNVKVAYLR
                     GFLFYKSIKFYYNISCREQ"
     gene            9010..10185
                     /gene="rpsA"
                     /locus_tag="ACGEXS_001122"
     CDS             9010..10185
                     /gene="rpsA"
                     /locus_tag="ACGEXS_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFF8012141.1"
                     /translation="MTEEFNESMINDIKEGDKVTGEVQQVEDKQVVVHINGGKFNGII
                     PISQLSTHHIDSPSEVVKEGDEVEAYVTKVEFDEENETGAYILSRRQLETEKSYSYLQ
                     EKLDNNEIIEAKVTEVVKGGLVVDVGQRGFVPASLISTDFIEDFSVFDGQTIRIKVEE
                     LDPENNRVILSRKAVEQEENDAKKDQLLQSLNEGDVIDGKVARLTQFGAFIDIGGVDG
                     LVHVSELSHEHVQTPEEVVSIGQDVKVKIKSIDRDTERISLSIKDTLPTPFENIKGQF
                     HENDVIEGVVVRLANFGAFVEIAPGVQGLVHISEIAHKHIGTPGEVLEPGQQVNVKIL
                     GIDEENERVSLSIKATLPNEDVVESDPSTTKAYLENEEEDNPTIGDMIGDKLKNLKL"
     gene            10407..11717
                     /gene="der"
                     /locus_tag="ACGEXS_001123"
     CDS             10407..11717
                     /gene="der"
                     /locus_tag="ACGEXS_001123"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019167598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFF8012142.1"
                     /translation="MTKPIVAIVGRPNVGKSTIFNRIVGERVSIVEDTPGVTRDRIYS
                     SGEWLTHDFNIIDTGGIEIGDAPFQTQIRAQAEIAIDEADVIIFMVNVREGLTQSDEM
                     VAQILYKSKKPVVLAVNKVDNMEMRTDVYDFYSLGFGEPYPISGSHGLGLGDLLDAVV
                     SHFGEEEEDPYDEDTIRLSIIGRPNVGKSSLVNAILGEDRVIVSNVAGTTRDAIDTEY
                     SYDGQDYVLIDTAGMRKKGKVYESTEKYSVLRALKAIERSNVVLVVIDAEQGIIEQDK
                     RVAGYAHEQGKAVVIVVNKWDTVEKDSKTMKKFEDEVRKEFQFLDYAQIAFVSAKERT
                     RLRTLFPYINEASENHKKRVQSSTLNEVVTDAISMNPTPTDKGRRLNVFYATQVAIEP
                     PTFVVFVNDVELMHFSYKRYLENQIRAAFGFEGTPIHIIARKRN"
     gene            11734..12732
                     /locus_tag="ACGEXS_001124"
     CDS             11734..12732
                     /locus_tag="ACGEXS_001124"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFF8012143.1"
                     /translation="MTKITVFGMGSFGTALANVLAENGHDVLMWGKNQDAVDELNTCH
                     TNKKYLKYAKLDVNIIATSDMTKAIQFADIYLMALPTKAMREVASQINDKLTSKKTFI
                     HVAKGIENGTFKRVSEMIEDSISPEYNAGIGVLSGPSHAEEVVVKQPTTVAASSKDKS
                     VSKLTQDLFMNDYLRVYTNDDLIGVELGGALKNIIAVASGIVAGIGYGDNAKAALMTR
                     GLAEISRLGEKLGADPMTFLGLGGIGDLIVTCTSTHSRNFTLGYKLGQGESMDQALSE
                     MNMVVEGIYTTKSVYHLAKEKNVDMPITNALYRVLFENISVKECVKDLMERDKKSE"
     gene            12903..13175
                     /locus_tag="ACGEXS_001125"
     CDS             12903..13175
                     /locus_tag="ACGEXS_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFF8012144.1"
                     /translation="MNKTDLINAVAEQADLTKKEAGSAVDAVFESIQNSLAKGEKVQL
                     IGFGNFEVRERAARKGRNPQTGKEIDIPASKVPAFKAGKALKDAVK"
     gene            13606..14178
                     /locus_tag="ACGEXS_001126"
     CDS             13606..14178
                     /locus_tag="ACGEXS_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenyl diphosphate synthase component 1"
                     /protein_id="HFF8012145.1"
                     /translation="METTVSKLERQIEERLKGVSEYESININHRLGKLLDLYDIPDVA
                     KVACLTIDTSMRHLDDITYNHLSKHSILIGDLISAHFYTLLAEINDLSFQNEISKAIV
                     EINELKSSLHHQALNDYEISQAIVKIETLFPYITLSHFGINIDESEIYNYLFEDMSDY
                     YPSYFKKYNQSEVKHYLHDIQKSYLKSRGN"
     gene            14181..14906
                     /locus_tag="ACGEXS_001127"
     CDS             14181..14906
                     /locus_tag="ACGEXS_001127"
                     /EC_number="2.1.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="demethylmenaquinone methyltransferase"
                     /protein_id="HFF8012146.1"
                     /translation="MADNKANKEQVHRVFQNISKKYDRLNNIISFEQHKVWRKRVMKD
                     MGVRKGTKALDVCCGTGDWTIALSKAVGPTGEVTGIDFSENMLEVGKEKTASMENVKL
                     VHGDAMELPFEDNSFDYVTIGFGLRNVPDYLVALKEMNRVLKPGGMVVCLETSQPTLP
                     VFKQMYALYFKFVMPIFGKLFAKSKEEYEWLQQSTFNFPGKEELKRMFEEAGFINVRV
                     RSFTGGVAAMHLGYKEKDNTKGD"
     gene            14908..15867
                     /locus_tag="ACGEXS_001128"
     CDS             14908..15867
                     /locus_tag="ACGEXS_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFF8012147.1"
                     /translation="MAKLNMNNEIKKVEQRLEKAIKSKDSVLEQASLHLLSSGGKRVR
                     PAFVILSSQFGKDEQTSEQTYQVAVALELIHMATLVHDDVIDKSDKRRGKLTISKKWD
                     QTTAILTGNFLLALGLEHLMAVKDNRVHQLISESIVDVCRGELFQFQDQFNSQQTIIN
                     YLRRINRKTALLIQISTEVGAITSQSDKETVRKLKMIGHYIGMSFQIIDDVLDFTSTE
                     KKLGKPVGSDLLNGHITLPILLEMRKNPDFKLKIEQLRRDSERKEFEECIQIIRKSDS
                     IDEAKAVSSKYLSKALNLISELPDGHPKSLLLSLTKKMGSRNT"
     gene            15959..16408
                     /gene="ndk"
                     /locus_tag="ACGEXS_001129"
     CDS             15959..16408
                     /gene="ndk"
                     /locus_tag="ACGEXS_001129"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFF8012148.1"
                     /translation="MERTFLMIKPDAVQRNLIGEVISRIERKGLKLVGGKLMQVPMEL
                     AETHYGEHQGKPFYNDLISFITSAPVFTMVVEGEDAVNVSRHIIGSTNPSEASPGSIR
                     GDLGLTVGRNIIHGSDSLESAEREINLWFNENEITSYASPRDAWLYE"
     gene            16617..>16699
                     /locus_tag="ACGEXS_001130"
     CDS             16617..>16699
                     /locus_tag="ACGEXS_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012149.1"
                     /translation="MKNIISIILGILMFLKLMELLYGAIFL"
BASE COUNT         6148 a         2406 c         3031 g         5114 t
ORIGIN      
        1 cctgcgacct gctagtatgt atcatattag tggctgatct agtgagattc tagagccgac
       61 agtatagtct ggatgggaga agatggaggt tttttgttgt gcaataatcc tcctattctt
      121 acgagatgaa tggaaggaga aaattgaata tgcaacaaaa taaacgtctt atcacaataa
      181 gtatgttgag cgcgattgcg tttgtgttaa cttttatcaa gtttcctata ccatttttgc
      241 caccatactt aactttagat tttagtgatg taccgtcact actagctaca tttacgtttg
      301 gaccagttgc cggtatcgta gttgcactgg ttaaaaattt attgaactac ttatttagta
      361 tgggcgatcc agttggacca tttgctaact ttttagcagg cgcaagtttc ttattaactg
      421 cttacgccat ctataaaaat aaacgttcaa caaaatcttt gattactgga ttaatcattg
      481 caacaatcgt tatgactatc gtgttgagta ttttgaacta tttcgttcta ttacctttgt
      541 acggtatgat atttaactta gctgatatcg caaataatct taaagtaatc attgtttcag
      601 gaattatacc attcaatatt attaaaggta tcgttatttc tattgtattt attttactat
      661 atagaaggct tgcgaatttc ttgaaaagaa tttaatcaaa ttaaagcaaa ataatataca
      721 cataataata aaaagcaggt gactatcaat aaacgatagc ttgcctgctt tttctataga
      781 acatttgtct aaaaaatcaa ttattcaaat tttaaagcgt ccccatcaaa tgattcgtct
      841 gcaattttaa tagaatctgt agggcatcca tcaattgcat cttccatatc ttcatataat
      901 tcctcaggta cttctgcagt accttggtta tcgtcaagga ttacgaaagc aataccttcg
      961 tcgtcgtaat catatatatc tggcgctgct gcaccgcatg caccacatgc aatacaagta
     1021 tccatatcaa cgattgtata ttttgccaat gtcttcgcct cctttgataa aaatgctaaa
     1081 atagtaatgt gactaaaatt ttagacagca tcatttttat tttcaaatta tccgttttac
     1141 agagtgaggg ttaaatttgc aacacattat aaaaacagca ttacaacaaa catttaacta
     1201 taaaacaaat aaaagtattt acaatatctt agttggtaag aaatctcacc aaaccttttt
     1261 tgacgcttgt agtcaacaac agttgtcatt atatcacagt ttaccactat taaaatatcc
     1321 gtcttttgag ctatttttag aaaaaatcaa tgaatttaat gctgaaatgg aaatcatgtt
     1381 gcatcctaga tatacatttg aaagcatggg gcaaacattt caagcaattc aactattagt
     1441 gcaaaccatg tctaatacca aacaacatgt ttttcatttt gtaccaatct ctcaaaataa
     1501 taagatacaa gaaactgtaa agattgtcta taactacatt aaagaaaacc aattacaaat
     1561 tgattttgaa aatgaactac ataacttgtt taaagcaata actttaaaag ggccatgtta
     1621 cttacattat tatttgcaag gctatgatga accaatgtat acgagacagc aagttagttt
     1681 aatagaaaag ctatctcaac agcaattgtt tgaatacgaa atgaataatt tagtgacaat
     1741 gatgtttgaa ttagaaagtg gagaatatac tattttatca aaaataataa tgaaacctac
     1801 attattaaat caaacttata ttacttatac aaaattgctt gaacaattca cgatggaaga
     1861 tatagcggct caacaacaag ttaaaatcaa tactatcgaa gatcatgtac ttgaaatctt
     1921 aatcaaaggt tacatgtcta attacgatga ttatgttgaa ctagaagatc aactccagtt
     1981 tttgaatttt tatcaacagc atcgtggcga acgattaaaa ttttacaaag aacaatttga
     2041 cacgttatca tattttcaat taaaagtatt aatcgttgga tttgaaagag gtgatctgaa
     2101 tgttgcatga tattttacga aacaaatttg gattcgagag ttttaaaccg ggacaacagg
     2161 aaattataga aagtataatg tctcaacaac acactctagg tatacttcca actggaagtg
     2221 gaaagagttt gtgttatcaa atacctacgt atttatcagg taagccgaca ttaattatct
     2281 caccgttaat atctttaatg gatgaccaag ttatgcagtt gaaaataaat ggagaaaaac
     2341 gtgtaacatg tattcactct ggtatggatg aaattgagaa aaagcataat attaaatgtt
     2401 tacgacatag ccgcttcatc tttctaagtc cagaatttct cctgcaaccg tcaaatttta
     2461 aattaatatc tatgatagac tttggcatga ttgttctaga tgaagcacat tgcctatctg
     2521 aatggggata tgatttcaga ccacattatg ctctaatagg aaaagtaaca aagcatttta
     2581 aagaagcggt tgtcttagca ttgacagcaa ctgcaccacc gcatttacaa gatgatttga
     2641 cggaaatgtt agcgattcaa ttcaatgtta ttaaaactac aatgaatcgc ccaaatataa
     2701 gctttaagca tcttaatttt catgatgatg aagataaaat tgaatggttg ctgccgtttc
     2761 tacaacagtc gggaccaacg attatttatg tctcatcgaa aaagatgtgt ctgaatttag
     2821 cgcaacttat ttatgattca ggttttctta caggtattta tcatggtgat atgaattatc
     2881 aagagcgaca cacagttcaa caacaatttt taaataatga tattccgatt atagtcgcaa
     2941 cgagtgcttt tggtatggga attaataaaa aagatattcg cacaatcatt cactttcatc
     3001 tttcaacaag tccttctaac tacattcaag aaattggccg tgcgggtcgc gatggtgaac
     3061 taagtcaggc aattagttta ttccaaccgg acgataaata tattttagaa acgttattat
     3121 ttgcagatat gataacagaa gaagatgtac aaaatttcga aataggagaa tttttagctc
     3181 ccgataaaca agccgttttg acaacgttgc aatcattcta tagtatcggc gccttgaaac
     3241 agatatttaa gcaatcattt aaacgaaagc aattaggatt ctttcgcatg attggctatt
     3301 gcaaattgga tcaatgtaga cggaagtatt tattagaatt tttcggtgaa tatccaccgg
     3361 cacaagatcg atgttgtgac aatgattcta atataactga tatcgcaatt ttaaataaga
     3421 agaaggtaat tagaagtatt ggatttgatg aaaagttgca aaatttattt ctcagatagt
     3481 attactttac taaaagaaaa ttgacaagct ataattagtg tatacacaat tgaaaaatga
     3541 ttgaaataat tttgaaaaat atacataaac atatgtcatg tgggtatatt ttatgtaaaa
     3601 tcattgtaat agaatagaaa ggaagatggc tatgtctaat aattttaaag atgactttga
     3661 aaaaaatcgt caatcgatag acacaaattc acatcaagac catacggaag atgttgaaaa
     3721 agaccaatca gaattagaac atcaggatac aatagagaat acggagcaac agtttccgcc
     3781 aagaaatgcc caaagaagaa aaagacgccg tgatttagca acgaatcata ataaacaagt
     3841 tcacaatgaa tcacaaacat ctgaagacaa tgttcaaaat gaggctggca caatagatga
     3901 tcgtcaagtc gaatcatcac acagtactga aagtcaagaa cctagccatc aagacagtac
     3961 acctcaacat gaagaggaat attataataa gaatgctttt gcaatggata aatcacatcc
     4021 agaaccaatc gaagacaatg ataaacacga tactattaaa aatgcagaaa ataacactga
     4081 gcattcaaca gtttctgata agagtgaagc tgaacaatct cagcaaccta aaccatattt
     4141 tacaacaggt gctaaccaat cagaaacatc aaaaaatgaa catgataatg attctgtaaa
     4201 acaagatcaa gatgaaccta aagaacatca taatggtaaa aaagcagcag ctattggtgc
     4261 tggaacagca ggtgttgcag gtgcagctgg tgcaatggct gcttctaaag ctaagaaaca
     4321 ttcaaatgac gctcaaaaca aaagtaattc tggcaaggcg aataactcga ctgaggataa
     4381 agcgtctcaa gataagtcta aagatcatca taatggcaaa aaaggtgcag cgatcggtgc
     4441 tggaacagca ggtttggctg gaggcgcagc aagtaaaagt gcttctgccg cttcaaaacc
     4501 acatgcctct aataatgcaa gccaaaacca tgatgaacat gacaatcatg acagagataa
     4561 agaacgtaaa aaaggtggca tggccaaagt attgttacca ttaattgcag ctgtactaat
     4621 tatcggtgca ttagcgatat ttggaggcat ggcattaaac aatcataata atggtacaaa
     4681 agaaaataaa atcgcgaata caaataaaaa taatgctgat gaaagtaaag acaaagacac
     4741 atctaaagac gcttctaaag ataaatcaaa atctacagac agtgataaat caaaagagga
     4801 tcaagacaaa gcgactaaag atgaatctga taatgatcaa aacaacgcta atcaagcgaa
     4861 caatcaagca caaaataatc aaaatcaaca acaagctaat caaaatcaac aacagcaaca
     4921 acaacgtcaa ggtggtggcc aaagacatac agtgaatggt caagaaaact tataccgtat
     4981 cgcaattcaa tactacggtt caggttcacc ggaaaatgtt gaaaaaatta gacgtgccaa
     5041 tggtttaagt ggtaacaata ttagaaacgg tcaacaaatc gttattccat aatataacta
     5101 tataaattgt aactgaactg ctatatacaa acgtgcatat tataagtatc tctaaatatt
     5161 ttggagaact taaattgcgc ttataagtat gtagcggttt tttcattttt caaagtttgt
     5221 tatttaacaa ggtcttgtct cgaatattgg catatcaatt taacttttta aatagtcatc
     5281 aaaaagataa aacaccacaa tcaacaaatt taacgaggaa gaataaaaaa taaatcaaca
     5341 tattaaattg tagtgttatt caactccgta gctaacaatt ctctatgcac attaaacaaa
     5401 ttgtcaaaaa tatatcataa atcttcaagc acagacttag cgcatcaatc actgaactgt
     5461 tataatagtt tgggattaaa ggaggccgaa acaatgcaaa aagttgaaag tatcataatt
     5521 ggtggagggc catgcggatt aagtgcggct attgaacaaa aaagaaaagg tattgatacc
     5581 ttaattattg aaaagggtaa tgtcgttgaa tcaatctaca attatcctac tcaccaaaca
     5641 tttttctcat caagtgataa attaagtatt ggggacgtac cgtttatcgt tgaagaaagt
     5701 aaaccaagac gtaatcaagc gctagtttat taccgagaag ttgtaaaaca tcatcaatta
     5761 aaagtaaatg catttgaaga agtattaact gttaaaaaaa tgaataataa atttactatt
     5821 actacgacga aagatgttta tgaatgtcga tttttaacaa tcgcgacagg ctattatggt
     5881 cagcataata cattagaagt tgaaggtgcg gatttaccta aagtgttcca ttattttaaa
     5941 gaggcacatc cgtattttga tcaagatgtt gtaattatcg gtggtaagaa ttcggctatc
     6001 gatgctgctt tggagttgga aaaagctggt gctaacgtga cggttctata tcgtggtgga
     6061 gattattcgc cttcaattaa accgtggata cttccaaatt tcacagcatt agtaaatcat
     6121 gaaaaaattg acatggaatt taatgctaat gttacccaaa taactgaaga tactgtgact
     6181 tatgaagtaa atggtgaaag taaaacgata cacaatgatt atgtatttgc gatgattggt
     6241 tatcatcccg attatgaatt tttaaaatct gtaggcattc aaattaatac aaatgaattt
     6301 ggaacagcgc ctatgtataa taaagaaaca tacgaaacaa atatcgaaaa ttgctatatt
     6361 gcaggtgtaa ttgctgcagg gaacgatgcg aataccattt ttattgaaaa tggtaaattc
     6421 cacgggggca ttattgctca aagcatgcta gctaagaaac aaacgccctt agaatcataa
     6481 aaataaaggt ctatgtacta aataacttag ttttacaacg actgacattc atgatatgtc
     6541 aaatgaggtt gatgactaat gattgtacat agaccttttt atgttacgta ttcattataa
     6601 ttcaaaatat gatttgattt cagctttatc taaattgttg cttaacgcga ctaataattt
     6661 taatcttgct tttggaccat tcaagccgtt agaaaaaata aaaccttgtt gtgcgagttg
     6721 gtaaccacca ccatcgtatg cgtaagttgg actcacaata ccattaaagg aacgtgaaac
     6781 tagcacaata ggtatattta aagatactaa ttgttgaatg ccttctaatg cgcttggagg
     6841 tatgttgcct tgtcctaacg cttcaataac cataccatcg ataccttctc gactataaaa
     6901 actaaaaatg tcacctggca tacccatata tgcttttact aatggtacat ttagtttgtc
     6961 attgacattt tccaatgctt gttggcgata tggcatatga tggaattgca cacgatcctt
     7021 tgtcaataca cctagcggac catgatttgg actttgaaat gtgtttgtat tagacgtatg
     7081 tgttttggta acattacgcg ccgtatgaat ttcatcatta aatacaacca tcacgccttt
     7141 atgacgggcc ttttcatcag aggcaacgcg aatagcggaa atataattat ataatccgtc
     7201 agaaccaatt tcattagacg agcgcattgc gccagtaata acaacaggtt gctcgatacc
     7261 taatattaaa tcaagtaaaa aggcagtttc ttctaacgta tcggtaccat gcgtgataac
     7321 gaaaccatca taatatttat ttgttactgc ttctaaaata atatccttta attgtttaac
     7381 atgttggatt gtcatatgag gtgatggtac attaaaagga tttaattcat ctatttgtgc
     7441 atattgattt atgacatctt gatgcattga aatagggttg atatcatttg ttactacttt
     7501 attagattgg tcttgtgaca tactaatggt gccaccagta tgaataacaa gtagatgttt
     7561 catatatttc ctcctatatt taatttacct aattatgata aaatattatt cataaaacga
     7621 caaggaaggg aaatgacgca tgaaagccat taatattgca ttagatggtc cagctgctgc
     7681 cggaaaaagt acaattgcga aacgtgtagc cagcgaacta tcaatgattt atgtcgatac
     7741 aggagcaatg tatcgtgcat taacatacaa atatttaaaa ttaaacaaaa ctgaggactt
     7801 tgcaaaacta gttgaccaaa caacattaga tttaacttat aaagcagata aaggtcaatg
     7861 tgtcatttta gataacgaag atgtaacaga ctttttaaga aataatgatg tgacgcaaca
     7921 tgtttcatac gttgcatcta aagagccagt acgttcattc gccgttaaaa aacaaaaaga
     7981 gttagctgca gaaaaaggta tcgtaatgga tggtcgcgat atcggaactg tagtgctacc
     8041 agatgcagat ttaaaagtat atatgattgc atcagttgaa gagcgagcag aaagaagata
     8101 taaagataat caattaagag gtatcgaatc aaattttgaa gatttaaaac gtgatattga
     8161 agctcgtgat caatatgaca tgaaccgtga aatatcacca ttaagaaaag cagatgatgc
     8221 agtgacatta gatacgacag gcaagtcgat tgaagaagtt actgacgaaa ttttagcgat
     8281 ggtgagtcaa attaaataat ctaataagtc ataactatct atttgtaatg tgcaacgctt
     8341 aacgcatata caaaatgaat gtgctgataa tgatttactc aaattaaaag gtgattttta
     8401 ttcaatgatg aatgaaagtt gcctttttat ttttggtaaa agttaatgcg tcagtgaatt
     8461 gtgtaagttt ttcaaaaagt aaaaagaaat aataaaggtg aattattaga attccaaaaa
     8521 taattcatta cattcataaa gcattttaca aatggtaaga aaatgagtgt tacaaatcta
     8581 aatattgcaa aagaagctga tttagtcaca aaaaatgtcc tatgtaataa ttcgagaaag
     8641 atgcactata tacggtcttc ttactattca aatgtaaaag ttgcttattt gcgtggcttt
     8701 ttgttttata aaagtataaa attttactat aatatatctt gtagagaaca atgaaatgac
     8761 agacttataa aacaaaatag taagctatgc taatttttat acaaaaatat gtataaacat
     8821 agcaaactgt attactttga tacaaaaatg gttgtaataa atatttatcg atatgacgac
     8881 ttgaatatga taaagtgaca tatttatgta tatgactatt tcgcaaaatg taatcgaggt
     8941 agaatttctt gacaattctg tcagtttata agatgttata aatatgtagt gtataaggag
     9001 gcaaacaaga tgactgaaga attcaatgaa tcaatgatta acgatattaa agaaggtgac
     9061 aaagtcactg gcgaggtaca acaagttgaa gacaagcaag ttgttgttca tatcaacggt
     9121 ggtaaattta atgggattat tcctattagt caactatcta cgcatcatat tgatagccca
     9181 agtgaagttg taaaagaggg cgacgaagtt gaagcatatg tcactaaagt tgagtttgat
     9241 gaagaaaatg aaactggagc ttacatctta tctagaagac aacttgaaac tgagaagtct
     9301 tatagttatt tacaagaaaa attagataat aatgaaatca tcgaagcgaa agtaacagaa
     9361 gtagttaaag gtggtttggt tgttgatgta ggacaaagag gttttgttcc ggcttcacta
     9421 atttcaacag acttcattga ggatttctct gtgtttgatg gacaaacaat tcgtattaaa
     9481 gttgaagaat tggatcctga aaataataga gtcattttaa gccgtaaagc agttgaacaa
     9541 gaagaaaacg atgctaaaaa agatcaatta ttacaatctt taaatgaagg cgatgttatt
     9601 gatggtaaag tagcgcgttt aactcaattt ggtgcattta tagacattgg cggtgttgat
     9661 ggtttagtgc atgtatctga actttctcac gaacatgttc aaacaccaga agaagtagtt
     9721 tcaattggtc aagatgttaa agttaaaatt aaatctattg atagagatac agaacgtatt
     9781 tcattatcaa tcaaagatac gttaccaaca cctttcgaaa atattaaagg tcaattccac
     9841 gaaaatgatg tcattgaagg tgtcgtagta agattggcaa actttggtgc atttgttgaa
     9901 attgcaccag gtgtacaagg acttgtacat atttctgaaa ttgcacacaa acacattggt
     9961 acgccaggtg aagtgttaga acctggtcaa caagtaaatg ttaaaatatt aggtattgat
    10021 gaagagaatg aaagagtatc actatctatt aaagcaacat taccaaacga agatgttgtt
    10081 gaaagtgatc cttctacgac taaggcgtac ttagaaaacg aagaagaaga taatccaaca
    10141 attggcgata tgattggtga taaacttaaa aatcttaaac tataatttaa tatttaatag
    10201 tcaactccac atgtttatga ttgcatgtgg agtattttta tgtaacaaaa tatactcgga
    10261 atgataacgt ggacaaattt aactaagtgt ttaaaaagat aaagttttaa gtgctgattt
    10321 ttatcattac agtaataaac tcattttgaa tacacagtct catgtgatat tattaaaaag
    10381 atataagaaa gagaggaagt tagcttatga ctaaacctat agtagctatt gtaggtaggc
    10441 ctaatgtagg taaatctaca atttttaata gaatagttgg agaacgtgtt tcgattgtgg
    10501 aagacacgcc aggtgtaaca cgagatcgta tttattcttc aggtgaatgg ttaacacatg
    10561 atttcaatat tattgataca ggtggtattg aaattggtga tgcaccattc caaacacaaa
    10621 ttagagcgca ggcagaaatc gccatagatg aagcggatgt tattattttt atggttaacg
    10681 tgcgtgaagg attgacacaa agcgatgaaa tggtcgctca aattttatac aaatctaaaa
    10741 aaccggtcgt attagcggtt aacaaagtag ataatatgga aatgcgtaca gacgtgtatg
    10801 atttctattc attaggattt ggtgaaccgt atccgatatc agggtcacat ggtttaggtc
    10861 ttggtgactt gttagatgca gttgtttctc attttggtga agaggaagaa gatccttatg
    10921 atgaagatac aattcgacta tccattattg gacgaccaaa cgtaggtaaa tcaagtttag
    10981 taaatgctat tttaggtgaa gatcgcgtta tcgtttctaa tgttgcaggg acaacgagag
    11041 acgctattga tacagagtat agttatgatg gacaagatta tgttttaatc gatactgctg
    11101 gtatgcgtaa aaaaggaaaa gtatatgaat caactgagaa atattcagta ttaagagctt
    11161 taaaagcgat tgaacgttca aatgttgttt tagtggtcat agatgcagaa caaggcatca
    11221 ttgaacaaga taaacgtgtt gcaggatatg cacatgaaca aggtaaagca gtcgtgattg
    11281 tcgtaaataa atgggatact gtggaaaaag atagtaaaac gatgaagaaa tttgaagatg
    11341 aagtacgtaa agaattccaa tttttagatt atgcacaaat tgcttttgtg tctgctaaag
    11401 aacgcacaag attacgtaca ttattccctt acatcaatga agcaagtgaa aaccataaaa
    11461 aacgtgttca aagttcaact ttaaatgaag ttgttactga tgcaatttcc atgaacccta
    11521 caccaacaga caaaggtaga cgtttgaatg tcttttatgc aacacaagtt gctatagaac
    11581 caccgacatt tgttgtattt gttaatgatg tagaattaat gcatttttct tataaacgct
    11641 atttagagaa tcaaatccgt gccgcttttg gttttgaagg tacaccaatt catattatag
    11701 ctcgaaagag aaattaacga ttgggggata acaatgacta aaattaccgt ttttggtatg
    11761 ggaagttttg ggacagccct tgccaatgtt cttgcagaaa atggacatga tgttttgatg
    11821 tggggtaaaa atcaagatgc tgttgatgaa ttaaatacat gtcatacaaa taaaaagtat
    11881 ttaaaatacg cgaaattaga tgttaacatc atcgctactt cagatatgac caaagcaatt
    11941 caatttgcag atatttactt aatggcttta cctactaaag caatgcgaga agttgcttct
    12001 caaattaatg ataagctgac ctctaaaaag acttttatac atgttgctaa aggtattgaa
    12061 aatgggacgt ttaaacgtgt gtcagaaatg attgaagatt ctatttcacc tgaatataat
    12121 gcaggtattg gcgtgttgtc agggccaagt catgcggaag aagttgtagt caagcaacca
    12181 actacagttg ctgcttcatc aaaagataaa agtgtaagta aattaacgca agatttattt
    12241 atgaatgatt atttgcgtgt gtacacgaat gatgacttga ttggtgttga acttggtggt
    12301 gcattgaaaa atatcatcgc agtagcaagt ggtatcgtag ctggaattgg ctacggtgat
    12361 aatgcaaaag ctgcattaat gactcgtggc ttagcggaaa ttagtagatt aggtgaaaag
    12421 ttaggtgccg atcctatgac atttctaggt ttaggtggta tcggtgactt aatcgttact
    12481 tgcacatcaa cacattctcg gaatttcaca ttaggatata aacttggaca aggtgaatca
    12541 atggatcaag cattatctga aatgaatatg gttgttgaag gtatttatac aactaaatca
    12601 gtttatcatt tagctaaaga aaaaaatgtg gatatgccaa ttacaaatgc attatataga
    12661 gtattatttg aaaatatctc agtaaaagaa tgcgtaaaag atttaatgga gcgcgataaa
    12721 aaatctgaat aaaatgtgta ttattacaca tatacggcta ataattacgt aaaatgaata
    12781 gaaatggctt aaattcaacg tttttgccac ataaatgatt gcgatttatg taatgtttgt
    12841 gttaaagttt taccataatg atataaacat atcattatca aacctcagga ggtgaatgtc
    12901 taatgaacaa aacagattta atcaatgcag ttgcagagca agctgattta actaaaaaag
    12961 aagctggttc agcagtagat gctgtattcg aatcaatcca aaactcactt gctaaaggtg
    13021 aaaaagtaca attaattggt ttcggtaact ttgaggtacg tgaacgtgct gcacgtaaag
    13081 gtcgtaaccc tcaaactggt aaagaaattg atatcccagc aagtaaagtt ccagcattca
    13141 aagctggtaa agcattaaaa gatgctgtaa aataatttta cttaaaaagc cctgaataag
    13201 ggctttttat tttgctttta atacttacaa cctgtacata aattgtaatg ttcttctaag
    13261 tttttaatct ttggaacatt cattgtttaa gcgttcaaaa tattacatga cataatagtt
    13321 ttgataaaaa tagcaactac aaaatataca tatgaatatt gagattaatt gttagcgttg
    13381 aatttactta aaaggtaacc atgtctacta tagtatttta cgttatttaa aaagatgaat
    13441 aatgtaaatg aagtaaaggt tattatgaga attacaaaag ctacataaat tactgttagt
    13501 ttaaattgaa atttaaaaat gataagtaat cttaatgctt caaaagattt taatttctta
    13561 catataatgt taatatgaat agtgataata tcgtgaggtg tagacatgga aacaactgtt
    13621 agcaaattgg aaagacaaat agaagaaaga ttaaagggcg tatcagaata tgaatccatt
    13681 aatattaatc atcggttagg taagttactt gatttatatg atattccaga tgttgccaaa
    13741 gtggcatgcc tcacaattga cacatcaatg agacaccttg atgacattac atataatcat
    13801 ttgtctaaac attccatttt gattggagat ttaattagtg cacattttta tacgctacta
    13861 gcagaaatca atgatttatc atttcaaaat gaaattagta aagcaattgt tgaaattaat
    13921 gaattgaaat cttcattaca tcatcaagct ttaaatgatt atgaaatttc tcaagcaata
    13981 gtaaaaattg aaacactttt cccatatatt acgttatctc atttcggtat caatattgat
    14041 gagtcggaaa tatataatta tttatttgaa gatatgtctg attactatcc atcatacttc
    14101 aagaaatata atcaaagtga agtaaagcat tacttacacg atatccaaaa aagttattta
    14161 aaaagtagag gtaattaagc atggctgaca ataaagcaaa taaagagcaa gtacatcgcg
    14221 tttttcaaaa tatttcaaaa aaatatgata gattaaataa tattattagt tttgagcagc
    14281 ataaagtatg gagaaaacgc gtcatgaaag acatgggtgt tagaaaaggg acgaaagcat
    14341 tagatgtttg ttgtggtact ggtgattgga caatcgcatt aagcaaagct gtaggaccaa
    14401 caggtgaagt tactggtatt gactttagtg agaatatgtt agaagtagga aaagaaaaaa
    14461 ctgcttcaat ggaaaatgtt aaacttgttc atggtgatgc aatggaattg ccgtttgaag
    14521 acaattcttt tgattatgta acaattgggt ttggattaag aaatgtgcca gactatttag
    14581 tcgcgttaaa agaaatgaat agagtactta aaccaggtgg tatggtggta tgtcttgaaa
    14641 cgagccaacc aactttgcca gtctttaaac aaatgtatgc actatacttt aaatttgtta
    14701 tgcctatttt tggaaaatta tttgcaaaat caaaagaaga atatgaatgg ttacagcaat
    14761 ctacgtttaa ttttcctgga aaagaagagt tgaagcgcat gtttgaagaa gcaggtttca
    14821 taaatgtaag agtacgtagt tttacagggg gcgttgctgc aatgcacctt ggctataaag
    14881 aaaaagataa taccaaaggt gattaacgtg gcaaagttaa acatgaacaa tgaaattaag
    14941 aaagtggaac aacgacttga aaaggcaata aaaagtaaag attctgtatt agaacaggca
    15001 tcattacact tattgtcttc tggtggtaaa agagtacgcc cagcatttgt tattctgagt
    15061 agccaatttg gcaaagatga acaaacgtct gaacaaacgt atcaagttgc agtcgcatta
    15121 gagttaattc atatggcaac acttgttcat gatgacgtta ttgataaaag cgacaagcgt
    15181 cgaggcaagt taaccatatc aaagaaatgg gatcagacaa ctgctatttt aactgggaat
    15241 tttttattgg cattaggact tgaacactta atggccgtta aagataatcg tgtacatcaa
    15301 ttgatatctg aatctatcgt tgatgtttgt agaggggaac ttttccaatt tcaagaccaa
    15361 tttaacagtc aacagacaat tattaattat ttacgacgta tcaatcgcaa aacagcactg
    15421 ttaattcaaa tatcaactga agttggtgca attacttctc aatctgataa agagactgta
    15481 cgaaaattga aaatgattgg tcattatata ggtatgagct tccaaatcat tgatgatgta
    15541 ttagacttca caagtaccga aaagaaatta ggtaagccgg tcggaagtga tttgcttaat
    15601 ggtcatatta cgttaccgat tttattagaa atgcgtaaaa atccagactt caaattgaaa
    15661 atcgaacagt tacgtcgtga tagtgaacgc aaagaatttg aagaatgtat ccaaatcatt
    15721 agaaaatctg acagcatcga tgaggctaag gcagtaagtt cgaagtattt aagtaaagct
    15781 ttgaatttga tttctgagtt accagatgga catccgaaat cactactttt aagtttgacg
    15841 aaaaaaatgg gttcaagaaa cacgtagtat ttatgaaaag tattgaaagc gctttaccaa
    15901 cctgttaata tataatagta atatacataa acctattaaa cacaggggga tacataaagt
    15961 ggaacgtaca tttttaatga ttaaaccaga tgcagtacaa agaaatctaa ttggtgaagt
    16021 aatttcaaga attgaaagaa aaggactaaa acttgtcggt ggtaaattaa tgcaagtacc
    16081 aatggaactt gctgaaacac attatggtga acaccaaggt aaaccatttt ataatgattt
    16141 aatttcattt attacatcag caccagtgtt cacaatggta gttgaaggtg aagatgcagt
    16201 taatgtatct agacatatta ttggcagcac caatccttca gaagcttcac caggatcaat
    16261 tagaggtgat ttaggtttaa ctgttggtag aaatatcatt cacggttcag attcattaga
    16321 gtctgctgaa cgtgaaatta atctatggtt taatgaaaat gaaattacta gctatgcttc
    16381 accacgtgat gcatggttat atgaataaaa tataaactgt aaacctttac gatttattta
    16441 taaaggtaga aagggttttg ttatgtggtt agtcattatg attatacata acaaggcccg
    16501 ttttttatgt tgtagtaaat tacttgaaaa attttatagt tttttggtaa cacgtattaa
    16561 aaagagagga atattcttta tcaaatgaaa ctaaacagag agaaggggtt gttaaaatga
    16621 agaatattat ttcgattatt ttggggattt taatgttctt aaaattaatg gaattactat
    16681 atggtgctat atttttaga
//
LOCUS       DAXWFK010000044        16694 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.044, whole genome shotgun sequence.
ACCESSION   DAXWFK010000044 DAXWFK010000000
VERSION     DAXWFK010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16694
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.044"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(627..1487)
                     /gene="folD"
                     /locus_tag="ACGEXS_001131"
     CDS             complement(627..1487)
                     /gene="folD"
                     /locus_tag="ACGEXS_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFF8012150.1"
                     /translation="MVAKILDGKQIAKDYRQGLQDQVEALKEKGFTPKLSVILVGNDG
                     ASQSYVRSKKKAAEKIGMISEIVHLEETATEEEVLNELNRLNNDDSVSGILVQVPLPK
                     QVSEQKILEAINPEKDVDGFHPINIGKLYIDEQTFVPCTPLGIMEILKHADIDLEGKN
                     AVVIGRSHIVGQPVSKLLLQKNASVTILHSRSKDMASYLKDADVIVSAVGKPGLVTKD
                     VVKEGAVIIDVGNTPDENGKLKGDVDYDAVKEIAGAITPVPGGVGPLTITMVLNNTLL
                     AEKMRRGIDS"
     gene            1688..2170
                     /gene="purE"
                     /locus_tag="ACGEXS_001132"
     CDS             1688..2170
                     /gene="purE"
                     /locus_tag="ACGEXS_001132"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFF8012151.1"
                     /translation="MKVAVIMGSSSDWKIMQESCNMLDYFEIPYEKQVVSAHRTPKMM
                     VQFASEARERGINIIIAGAGGAAHLPGMVASLTTLPVIGVPIETKSLKGIDSLLSIVQ
                     MPGGIPVATTAIGAAGAKNAGILAARMLSIQNPSLVEKLNQYESSLIQKVEDMQNELQ
                     "
     gene            2157..3281
                     /gene="purK"
                     /locus_tag="ACGEXS_001133"
     CDS             2157..3281
                     /gene="purK"
                     /locus_tag="ACGEXS_001133"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFF8012152.1"
                     /translation="MNFNKLKFGATIGIIGGGQLGKMMAQSAQKMGYKVVVLDPSEDC
                     PCRYVAHEFIQAKYDDEKALNQLGQKCDVITYEFENISAQQLKLLCEKYNIPQGYQAI
                     QLLQDRLTEKETLKSAGTKVVPFISVKESTDIDKAIETLGYPFIVKTRFGGYDGKGQV
                     LINNEKDLQEGFKLIETSECVAEKYLNIKKEVSLTVTRGNNNQITFFPLQENEHRNQI
                     LFKTIVPARIDKTAEAKEQVNKIIQSIHFIGTFTVEFFIDSNNQLYVNEIAPRPHNSG
                     HYSIEACDYSQFDTHILAVTGQSLPNSIELLKPAVMMNLLGKDLDLLENEFNEHPEWH
                     LHIYGKSERKDSRKMGHMTVLTNDVNQTEQDMYAKFEGSN"
     gene            3285..3989
                     /gene="purC"
                     /locus_tag="ACGEXS_001134"
     CDS             3285..3989
                     /gene="purC"
                     /locus_tag="ACGEXS_001134"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFF8012153.1"
                     /translation="MTLLYEGKAKRIFSTNQENELRVEYKDEVTAGNGAKKDTMAGKG
                     RLNNQITSIIFKYLQENGIESHFIKQLSETEQLVKPVKIIPLEVVVRNIASGSITKRL
                     GFENGEVFREPLVEFFYKNDALNDPLITDDHVKLLNIASDEDIEILKSKALKINNVLK
                     QLMDAMNLKLVDFKIEFGKTETGQILLADEISPDTCRIWDKATNANFDKDVYRNNTGS
                     LIETYQIFLNKLEDLK"
     gene            3989..4252
                     /gene="purS"
                     /locus_tag="ACGEXS_001135"
     CDS             3989..4252
                     /gene="purS"
                     /locus_tag="ACGEXS_001135"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFF8012154.1"
                     /translation="MKTIELHITLQPQVLDTQGQTLTRAVHDLGYAQVNDIRVGKVLY
                     MTVDEVSDEKVHNIITTLSEKLFANTVIEEYSYKVLDDEKENA"
     gene            4254..4925
                     /gene="purQ"
                     /locus_tag="ACGEXS_001136"
     CDS             4254..4925
                     /gene="purQ"
                     /locus_tag="ACGEXS_001136"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HFF8012155.1"
                     /translation="MKFAVLVFPGSNCDRDMFNAAIKSGVEAEYVDYRETSLSGFDGV
                     LIPGGFSFGDYLRSGAMASVAPIISEVKRLAAEGKPVLGVCNGFQILTEIGLLPGALL
                     HNDSHLFISRNEELEIVNNQTAFTNLYEQGEKVIYPVAHGEGHYYCTDEIYQQLKANN
                     QIILKYVNNPNGSYDDIAGIVNEKGNVCGMMPHPERALETLLGTDSGVKLFEAMVKSW
                     REQHV"
     gene            4918..7107
                     /gene="purL"
                     /locus_tag="ACGEXS_001137"
     CDS             4918..7107
                     /gene="purL"
                     /locus_tag="ACGEXS_001137"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFF8012156.1"
                     /translation="MSKFIEPSVEEIKLEKVYQDMGLSDQEYEKVCDILGRQPNFTET
                     GIFSVMWSEHCSYKHSKPFLKQFPTSGDHVLMGPGEGAGVVDIGDNQAVVFKVESHNH
                     PSAIEPYQGAATGVGGIIRDIVSIGARPINLLNSLRFGELDNKQNQRLLKGVVKGIGG
                     YGNCIGIPTTAGEIEFDERYDGNPLVNAMCVGVINHDMIQKGTAKGVGNSVIYVGLKT
                     GRDGIHGATFASEELTEESESKRPSVQIGDPFVGKKLMEATLEAITFDELVGIQDMGA
                     AGLTSSSSEMAAKGGSGLHLRLEQVPTREPGISPYEMMLSETQERMLLVVEKGTEQKF
                     LDLFDKHELDSAVIGEVTDTNRFVLTYDDEVYADIPVEPLADEAPVYILEGEEKDYNT
                     SKNDYTHIDVKDTFFKLLKHPTIASKHYLYDQYDQQVGANTIIKPGLQASVVRVEGTN
                     KAIASTIDGEARYVYNNPYEGGKMVVAEAYRNLIAVGATPLAMTDCLNYGSPEKKEIY
                     QQLIDSTKGMAEACDILKTPVVSGNVSLYNETKGTSIFPTPVVGMVGLIENVNYLNDF
                     EPQVGDKLYLIGDTKDDFGGSQLEKLIYGKVNHEFESLDLSSEVEKGESIKTAIREGL
                     LSHVQTVGKGGLLITLAKLSAHYGLGLKSSIDITNAQLFSETQGRYVVSVKSSKTLNI
                     DNAIEIGLLTDSDNFKVTTPYTEISENVSDIKQIWEGAIAQCLTTQD"
     gene            7086..8570
                     /gene="purF"
                     /locus_tag="ACGEXS_001138"
     CDS             7086..8570
                     /gene="purF"
                     /locus_tag="ACGEXS_001138"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFF8012157.1"
                     /translation="MFNYSGLNEECGVFGIWNHPEAAQLTYMGLHSLQHRGQEGAGIV
                     VSDQNELKGERGLGLLTEAIKDDQMERLKGYQHAIGHVRYATSGNKGIENIQPFLYHF
                     YDMSVGICHNGNLINAKSLRQNLEKQGAIFHSSSDTEVIMHLIRRSKAPTFEEALKES
                     LRKVKGGFTFAILTKDALYGAVDPNAIRPLVVGKMKDGTYILASETCAIDVLGAEFVQ
                     DIHAGEYVVINDKGITVKSYTHHTTTAISAMEYIYFARPDSTIAGKNVHAVRKASGKK
                     LAQESPVNADMVIGVPNSSLSAASGYAEEIGLPYEMGLVKNQYVARTFIQPTQELREQ
                     GVRVKLSAVKDIVDGKNIILVDDSIVRGTTIRRIVKMLKDSGANKVHVRIASPEFMFP
                     SFYGIDVSTTAELISASKSPEEIKDYIGADSLAYLSVDGLIESIGLDYDAPYSGLCVE
                     SFTGDYPAGLYDYEANYKAHLSHRQKQYISKNKHFFDSEGNLNV"
     gene            8563..9591
                     /gene="purM"
                     /locus_tag="ACGEXS_001139"
     CDS             8563..9591
                     /gene="purM"
                     /locus_tag="ACGEXS_001139"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFF8012158.1"
                     /translation="MSKAYEQSGVNIHAGYEAVERMSSHVKRTMRKEVIGGLGGFGAT
                     FDLSQLNMTAPVLVSGTDGVGTKLKLAIDYGKHDSIGIDAVAMCVNDILTTGAEPLYF
                     LDYIATNKVVPEVIEQIVKGISDACVETNTALIGGETAEMGEMYHEGEYDVAGFAVGA
                     VEKDDYVDGSEVKEGQVVIGLASSGIHSNGYSLVRKLINESGIDLASNFDNRPFIDVF
                     LEPTKLYVKPVLALKKEVSIKAMNHITGGGFYENIPRALPAGYAARIDTTSFPTPKIF
                     DWLQQQGNIDTNEMYNIFNMGIGYTVIVDEKDVSRALKILAEQNVEAYQIGHIVKNES
                     TAIELLGV"
     gene            9594..10160
                     /gene="purN"
                     /locus_tag="ACGEXS_001140"
     CDS             9594..10160
                     /gene="purN"
                     /locus_tag="ACGEXS_001140"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFF8012159.1"
                     /translation="MVKIAIFASGSGSNFENIVEHVESGKLENIEVTALYTDHQNAFC
                     IDRAKKHDIPVYINEPKQFDSKAAYEQHLVTLLNEDKVEWIILAGYMRLIGPDLLASF
                     EGKILNIHPSLLPKYKGIDAIGQAYHSGDTITGSTVHYVDSGMDTGEIIEQRKCDIRP
                     DDSKEQLEEKVKKLEYELYPSVIAKIVK"
     gene            10175..11653
                     /gene="purH"
                     /locus_tag="ACGEXS_001141"
     CDS             10175..11653
                     /gene="purH"
                     /locus_tag="ACGEXS_001141"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFF8012160.1"
                     /translation="MKKAILSVSNKTGIVEFAKALTQLNYELYSTGGTKRILDEANVP
                     VRSVSDLTHFPEIMDGRVKTLHPAVHGGILADRNKPQHLNELSEQHIDLIDMVVVNLY
                     PFQQTVANPDVTMDEAIENIDIGGPTMLRAAAKNYKHVTTIVHPADYQEVLTRLRNDS
                     LDESYRQSLMIKVFEHTAEYDEAIVRFFKGDKETLRYGENPQQSAYFVRTSNAKHTIA
                     GAKQLHGKQLSYNNIKDADATLALVKKFDTPATVAVKHMNPCGVGIGDTIEQAFQHAY
                     EADSQSIFGGIVALNRAVTPELAEQLHSIFLEVIIAPKFTDEALDILKQKKNVRLLEI
                     DMTIDSNEEEFVSVSGGYLVQDKDNYVVPKEEMKVVTEVAPTDEQWEAMLLGWKVVPS
                     VKSNAIILSNNKQTVGIGAGQMNRVGAAKIALERAIEINDHVALVSDGFFPMGDTVEL
                     AAQHGIKAIIQPGGSIKDQDSIDMANKHGIAMVVTGTRHFKH"
     gene            11675..12922
                     /gene="purD"
                     /locus_tag="ACGEXS_001142"
     CDS             11675..12922
                     /gene="purD"
                     /locus_tag="ACGEXS_001142"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFF8012161.1"
                     /translation="MNVLVIGAGGREHALAYKLNQSNLVKQVFVIPGNEAMTPIAEVH
                     TEISESDHQAILDFAKRQNVDWVVIGPEQPLIDGLADILRANGFKVFGPNKQAAQIEG
                     SKLFAKKIMEKYNIPTADYKEVERKKDALTYIENCELPVIVKKDGLAAGKGVIIADTI
                     EAARSAIEIMYGDEEEGTVVFETFLEGEEFSLMTFVNGDLAVPFDCIAQDHKRAFDHD
                     EGPNTGGMGAYCPVPHISDDVLKLTNETIAQPIAKAMLNEGYQFFGVLYIGAILTKDG
                     PKVIEFNARFGDPEAQVLLSRMESDLMQHIIDLDEGKRTEFKWKNESIVGVMLASKGY
                     PDAYEKGHKVSGFDLNENYFVSGLKKQGDTFVTSGGRVILAIGKGDNVQDAQRDAYKK
                     VSQIQSDHLFYRHDIANKALQLK"
     gene            complement(13187..13993)
                     /locus_tag="ACGEXS_001143"
     CDS             complement(13187..13993)
                     /locus_tag="ACGEXS_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFF8012162.1"
                     /translation="MYEQWKVRQSFVDDVNIITKLFLGIILFFFIIFVHNFDFMLYIT
                     ILMLVFLLMFNGTQFKITAIFITISIGFALLSSLFMILYGNGSHTILKFGFIHITTES
                     LVRGLHVSLRTIAISFFGILIALTSQVVMIFYSLMQHLKVKSKVAYAFMAAIRMIPLM
                     ISSLIQLRRSLKMRYQMIDAANYRGLKRFKHLIIPLLSQNIRKAHQLSVAMESKGFRD
                     GPRTYYYNVPFSYKDIIFIIVLIAIITMAYYCSITLPITGIDDVRLGRVG"
     gene            complement(13986..15386)
                     /locus_tag="ACGEXS_001144"
     CDS             complement(13986..15386)
                     /locus_tag="ACGEXS_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8012163.1"
                     /translation="MLKVSDLRLKYPSGQRKIFDHLNITIQDKEKVLLLGPSGCGKST
                     LLNVLSGIVPNLIELPMKYDELIVDPLSGVIFQDPDSQFCMPKVYEELAFVLENRQLP
                     REDMDALIINALNMVNLNVTPETYIKDLSGGMKQKLAIVETILQQSKTLFLDEPTAML
                     DVQATEDLWTKLIELWEDQTVVIVEHKVKHIWNHVDRVILMDYNGNIIADECPEIILQ
                     KYVHLLSEYGVWHPRAWEFAPSRVDFPTTNSHLLQFKNGRIIRGKSTLLSFSDLEIGL
                     GEWITITGANGSGKTTLLESIMQLIKYQGDVYFENQRLTKIKHAAKHMYLVYQNPELQ
                     FITNSVYDEINIHFNHLSKDQSDDETIQLLKLLDLQNVKDQHPYELSIGQKRRLSVAT
                     ALSSKADIIFLDEPTFGLDSHNTFQLIKLFQKRINLGQSIVMVTHDDEIIERYPSRRL
                     KISDGALLDCDGDTNV"
     gene            complement(15401..15976)
                     /locus_tag="ACGEXS_001145"
     CDS             complement(15401..15976)
                     /locus_tag="ACGEXS_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFF8012164.1"
                     /translation="MSKGLKLSEILVTVLISVVFAVIYNLWWFVYNGVQATGLHLEQL
                     TNGVWFMAAIVCYLIIPKPGIALLAEFAAGAGETIIMGRFDIPTIVYAFIQGLACELV
                     FAIFKYQSRSVMVAMLAGFCTAIAAFPIDYFYGYLNEVAGWNLTLFIVFRLISGAVVA
                     GVLSYLLVKALDKTGVTKLFRPAAKEDYDNL"
     regulatory      complement(16053..16153)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(16560..16694)
                     /gene="graF"
                     /locus_tag="ACGEXS_001146"
     CDS             complement(16560..16694)
                     /gene="graF"
                     /locus_tag="ACGEXS_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance-associated protein GraF"
                     /protein_id="HFF8012165.1"
                     /translation="MSNENQNKKAAEKAKEVEEKLKDKKEEKTEDINQTKQDIQDTLN
                     "
BASE COUNT         5801 a         2493 c         3188 g         5212 t
ORIGIN      
        1 ataatttagg gataatttga aatcaaatat aactttattc gacagtttga acttaaattt
       61 ttgtgtatca aaacaataag aaaagggagg cgaaaatgat aaagaaccac taatgattaa
      121 ctatgtagta gtactttatc atagtcacag cttctgtgtt ggggcccatc cccaacttgc
      181 acattattgt aagctgactt ttcgtcagct tctttgttgg ggcccctgac tagaattgaa
      241 aacttgttac aagcttattt tcattcaatt aactactgtc tatataaaat tgttgagcca
      301 agtacaaagt gaaatgctcc cttcaaagcc ttcatttatt caatgtctac tttgaaggga
      361 gcatttgatc gattaatgtc ccagattttt cttgttattc attgcaattg caaaataata
      421 tttaatgtgg gttaaaagac ttcattaaaa ctcagttatt agggagtggg acagaaatga
      481 tattttcgta aaatttattt cgtcgtccca ccccggcaag gttgactaga attgaaaaaa
      541 gcttgttaca agcgaatttt cgttcagtca actactgcca ttataacttt gtagagcata
      601 gaacattgat ttatgtctca ggctctttac gaatcaatac ctcgacgcat tttttctgca
      661 agcaaagtat tatttaatac catagtaatt gttaatggac caacgccacc aggaactggt
      721 gtaatagctc cagcaatttc tttaaccgca tcataatcaa cgtcaccttt taatttgcca
      781 ttttcatctg gcgtattgcc aacatcgata attactgctc cttctttgac cacatctttt
      841 gttactaaac caggcttacc aactgcactg acaatgacat cagcatcttt taaatatgat
      901 gccatatctt ttgaacgaga atgtaagatt gttactgatg catttttttg aagtagtaac
      961 ttagaaactg gttgtccgac aatatgactt cgtccaatta caactgcatt tttaccttct
     1021 aaatcaatat cagcatgttt taatatttcc atgatgccga gcggtgtgca aggtacaaaa
     1081 gtttgttcat cgatatataa tttccctata tttattggat gaaaaccgtc cacatctttt
     1141 tcaggattga ttgcttctaa tattttctgt tcgctaactt gttttggtaa tggtacttgt
     1201 accaaaatac cacttacaga atcatcatta tttagtctat ttagttcgtt taatacttct
     1261 tcttcagtag ctgtttcttc caaatgtacg atttctgaaa tcataccaat tttttcagct
     1321 gctttctttt ttgatctaac ataactttga ctagcgccat cattaccaac taatataacg
     1381 gataatttag gtgtaaaacc cttttctttt agcgcttcaa cttgatcttg taacccctgt
     1441 ctgtagtctt tggcaatttg tttaccatct aaaattttag caaccataaa aacttctcct
     1501 cctattgtca atatcctaac tttgttagtt tataaaacta atttacatag caaaagacgt
     1561 aaattcaaac aaaaaatcaa aataaagttc gatttttgat tgaaaaagca gaaattgctt
     1621 gttatgctat atctataata tacaactaaa gtaattcaca attcaaactt ttgaaagggt
     1681 gtacaaagtg aaagtagcag tcattatggg cagttcttcc gattggaaaa ttatgcaaga
     1741 gagttgtaac atgttggatt attttgaaat tccgtacgaa aaacaagtag tatccgcaca
     1801 tcgtacgcca aaaatgatgg ttcaatttgc ttctgaagcg agagaaagag gtataaacat
     1861 tatcattgca ggcgctggcg gtgcggcaca tttaccaggt atggttgcat cattaacgac
     1921 gctaccagtt attggagtgc cgattgaaac aaaaagttta aagggtatag attctttatt
     1981 atcaattgtt caaatgccag gaggtattcc ggttgcaacg actgcaattg gtgcagcagg
     2041 tgctaaaaac gcaggtatac ttgcagcaag aatgttaagt attcaaaatc cttctttagt
     2101 tgaaaaacta aatcagtatg aatcttcgtt aattcaaaaa gtggaggaca tgcaaaatga
     2161 acttcaataa attaaagttt ggtgcgacta ttggcattat tggtggtggt cagcttggaa
     2221 agatgatggc acaatcagct caaaaaatgg gttataaagt ggttgtattg gatccttctg
     2281 aagattgtcc atgtagatac gttgcacacg aatttataca agccaagtat gacgatgaaa
     2341 aggcactcaa tcaattagga caaaaatgtg atgtgattac ttatgaattt gaaaacattt
     2401 cagcccaaca attaaaacta ttatgtgaaa agtacaatat tccgcaaggt taccaagcta
     2461 tacagttatt acaagatcgc ttaactgaaa aagaaacatt aaaaagtgct ggtaccaaag
     2521 ttgtcccgtt catttcagta aaagaatcta cagatattga caaagcaatt gaaacattag
     2581 gatatccttt tattgtaaaa actagatttg gtggctacga tggcaaaggt caagttttaa
     2641 ttaacaacga aaaagactta caagaaggtt ttaaattaat tgaaactagt gaatgcgtag
     2701 ctgaaaaata tttgaatatc aagaaagaag tatctcttac tgttacaaga ggaaacaaca
     2761 atcaaatcac ttttttccca ttacaagaaa atgagcatag aaatcaaata cttttcaaaa
     2821 caattgttcc agcgagaata gataaaacag ctgaggcgaa agagcaagtt aataaaatta
     2881 tccaatcgat tcatttcatt ggaacattta cagttgaatt ttttatagat agtaacaacc
     2941 aattgtatgt gaacgagata gcaccaaggc ctcacaattc cggacattat tcaattgaag
     3001 catgtgatta ttcacaattt gatactcata ttttagcagt taccggacaa tcattaccaa
     3061 attcaattga attattaaag ccagcagtca tgatgaactt actaggtaaa gatttagatt
     3121 tattggaaaa tgaatttaat gaacatccag agtggcactt acatatttat ggtaagtctg
     3181 agcgtaaaga tagcagaaaa atggggcata tgactgtact aacgaatgat gtaaaccaaa
     3241 ctgaacaaga tatgtacgct aaatttgagg ggagtaatta aagcatgaca ttattatatg
     3301 aaggaaaagc gaagcgcatt ttctcaacaa atcaagaaaa tgaattaaga gttgaatata
     3361 aagatgaagt tactgctgga aacggggcta agaaagacac aatggcaggt aaggggcgat
     3421 taaataatca aattacttct attatattta aatatttaca agaaaatgga atagaaagtc
     3481 actttattaa acaattatct gaaacagaac aattagttaa gcctgtgaaa ataattccat
     3541 tagaagtagt tgttcgtaat attgctagtg gatctattac aaagcgttta ggttttgaaa
     3601 atggtgaagt ttttagagaa ccacttgtag aatttttcta taaaaatgat gcgttaaatg
     3661 atccgttgat aacggatgac catgttaaat tgctcaatat agcatcagat gaagatattg
     3721 aaatactaaa atccaaagca ttaaagatta ataatgtgtt gaaacaatta atggatgcta
     3781 tgaatttaaa attagtagat tttaaaatcg aatttggaaa gactgagact ggtcaaattt
     3841 tgttagcgga tgaaatatct ccagatacat gtcgaatttg ggataaagct accaatgcaa
     3901 actttgataa agatgtatat agaaataaca ctggatcact gattgaaaca tatcaaatat
     3961 ttttaaacaa attggaggat ttaaaataat gaaaacaatt gaactacata tcacattaca
     4021 accacaagta ttagatacgc aaggacaaac gcttactcga gctgtacatg acttaggtta
     4081 tgcacaagtg aatgatattc gtgtaggaaa agtattatat atgacagtgg atgaggttag
     4141 tgatgaaaag gtacacaaca ttattacaac tctaagtgaa aaattgtttg caaatacagt
     4201 gattgaagaa tatagctata aagtgttaga tgatgaaaag gagaatgcat aaaatgaaat
     4261 ttgcggttct tgtttttcca ggttcgaatt gtgatagaga catgtttaat gctgctatta
     4321 aaagtggtgt tgaagcggaa tatgtagatt atagagaaac atcactaagt ggatttgatg
     4381 gcgtacttat tcctggtgga ttttcattcg gggattactt aagatctggg gcaatggcta
     4441 gtgtagcgcc gattatttcg gaagttaaac gtcttgcagc tgaaggtaag ccagtattag
     4501 gtgtttgtaa tgggtttcaa attttaactg aaataggctt attacctggt gcattattgc
     4561 ataacgattc acatttattt attagtagaa atgaagagtt agaaatagtg aataatcaaa
     4621 cggcatttac aaatctttat gaacaaggtg aaaaagttat atatcctgta gctcacggtg
     4681 aaggtcatta ttattgtact gatgaaatat atcaacaatt aaaagctaac aatcaaatta
     4741 ttctgaaata tgtgaataat ccgaacggtt catatgatga tattgcagga attgttaacg
     4801 aaaaaggcaa tgtatgtggc atgatgccac atcctgaaag agctttagaa acgttgttag
     4861 gtactgatag tggtgtgaaa ttatttgaag cgatggtaaa aagttggagg gaacaacatg
     4921 tctaaattta tcgaaccaag cgttgaagaa attaaacttg aaaaagtata tcaagatatg
     4981 ggattaagtg atcaagaata tgaaaaagtt tgcgatattt taggcagaca acctaacttt
     5041 acagaaacag gtatcttttc tgttatgtgg agtgaacatt gctcttataa acattctaaa
     5101 ccgtttttaa agcaatttcc tacgtcaggt gaccatgtgc ttatggggcc tggtgaaggt
     5161 gcaggggtag tcgatatagg tgataatcaa gccgtagtat ttaaagtaga gtctcacaat
     5221 catccatcag caattgaacc atatcaaggg gctgctacag gcgttggtgg aatcattcgt
     5281 gacattgtct ctattggggc tagacctatt aatttgttaa acagtcttag atttggagaa
     5341 ttagataata aacaaaacca aagattactt aaaggtgttg taaagggtat cggaggttat
     5401 ggtaactgca ttggtattcc aacaactgct ggtgaaatcg aatttgatga acgttatgat
     5461 ggcaatccac ttgttaatgc aatgtgtgtt ggtgttatca atcacgacat gattcaaaaa
     5521 ggcacagcaa aaggtgtagg taattcggtc atttatgttg gtttgaaaac tggtcgagat
     5581 ggtattcatg gtgctacttt tgcatctgaa gaattgacgg aagaaagcga aagtaaacga
     5641 ccttctgtac aaatcggtga tccatttgta ggtaaaaaat taatggaagc aacacttgaa
     5701 gcaattacat ttgatgaatt agttggtatt caagatatgg gtgctgctgg tttaacatct
     5761 tcatcgtctg aaatggcggc aaaaggtggt agtgggttac atttgagatt agaacaagtg
     5821 ccaacacgtg agccaggtat ttctccttat gaaatgatgc tttcagaaac tcaagaacgt
     5881 atgttactag ttgttgaaaa aggtactgaa caaaaattct tagatttatt tgataagcac
     5941 gaattggata gtgctgttat aggtgaagtt acagatacaa atcgttttgt tttaacatat
     6001 gatgacgaag tttatgctga cattccagtt gaaccactag ctgatgaagc acctgtatat
     6061 attttagaag gagaagaaaa agattataat acttctaaaa atgattatac acacatcgat
     6121 gttaaagata ctttctttaa attacttaag catccgacta tagcatctaa acactattta
     6181 tatgatcaat acgaccaaca agttggtgcc aatacgataa ttaagccagg acttcaagca
     6241 tcggtagtac gtgtggaagg cacaaataag gcaattgctt caacaattga tggtgaagcg
     6301 cgttatgtat ataacaatcc atatgaaggt ggaaagatgg tagtagctga agcttatcga
     6361 aatttaattg ccgtgggtgc aacaccatta gcaatgacag attgtttaaa ttatggttct
     6421 cctgaaaaga aagaaatcta tcaacagttg atagattcaa cgaaaggtat ggcagaagca
     6481 tgcgacattc ttaagacacc agtagtttct ggtaatgtat ctttatataa cgaaacgaaa
     6541 ggtacttcta ttttcccaac accagttgtt ggaatggtag gtttgattga aaatgtaaat
     6601 tatttaaatg attttgaacc tcaagttgga gataaattat atttaatcgg tgatactaag
     6661 gacgactttg gtggtagtca acttgaaaag ttaatttatg gcaaagttaa tcatgaattt
     6721 gagtcattag atttgagttc agaagttgaa aaaggtgaat caatcaagac cgctattcgt
     6781 gaaggactat tatcacatgt tcaaacagtt ggtaaaggtg gcttactgat taccttagct
     6841 aaactaagtg cgcattacgg tttaggatta aaatcttcaa tagatataac aaatgcacaa
     6901 ttgtttagtg agacgcaagg ccgatatgtt gtttctgtta aatcaagtaa aactttaaat
     6961 attgataatg caatagaaat tggactttta acagatagtg ataatttcaa ggtaacaaca
     7021 ccatatacag agattagtga aaatgtttca gatattaaac aaatatggga aggggcaatt
     7081 gctcaatgtt taactactca ggattaaacg aagaatgtgg cgtgtttggt atttggaatc
     7141 atcctgaagc agcgcaacta acatatatgg gacttcatag tttgcaacat cgtggtcaag
     7201 aaggtgcagg tatagttgtt tctgatcaaa atgaattaaa aggcgagcga ggattaggct
     7261 tactaactga agcgattaaa gatgatcaaa tggaacgatt aaaaggatat caacatgcaa
     7321 ttggtcacgt ccgttatgct acttcaggta ataaaggtat tgaaaatatt caaccgtttc
     7381 tgtatcactt ttatgatatg agtgtaggta tttgtcataa tggtaacctc attaatgcta
     7441 aatcattgcg tcagaattta gaaaaacaag gtgctatctt ccattcgtct tctgatactg
     7501 aagtcattat gcatttgata cgtcgaagta aagctcctac ttttgaggaa gcgttgaaag
     7561 aaagtttgcg aaaagttaaa ggcggtttta catttgcgat tttaactaaa gatgcgttat
     7621 atggcgcagt agatccaaat gctatcagac cacttgttgt aggtaaaatg aaagatggga
     7681 catacatcct tgcaagtgaa acatgtgcaa tagatgtgtt aggtgcagaa tttgttcaag
     7741 atattcatgc aggtgaatat gtcgtgatta acgataaagg tattacagtt aaatcttata
     7801 cacatcatac gacaactgca atttctgcga tggaatatat ttattttgct agaccagact
     7861 caacaatagc tggtaaaaat gtccatgcag tacgtaaagc ttctggtaaa aaattagccc
     7921 aagaaagccc tgtaaatgct gatatggtca tcggtgtacc caattcatcg ctatcagctg
     7981 cgagtggtta tgctgaagaa ataggtttgc catatgaaat gggactagtt aaaaatcaat
     8041 atgttgcaag aacatttatt caaccaactc aagaattacg tgagcaaggt gtgagagtga
     8101 agttatctgc ggtaaaagat atagtagatg ggaaaaatat cattcttgtt gatgattcca
     8161 ttgttcgcgg tacgacaatt cgacgcattg tgaaaatgtt aaaagattct ggtgcaaata
     8221 aagtgcatgt gcgtatagca tcaccggaat ttatgtttcc aagtttttac ggaatcgatg
     8281 tttcaactac ggcagaatta atttctgcaa gcaaatcacc tgaagaaatt aaagattata
     8341 ttggcgctga ttcattagca tatctatctg tagatgggtt aattgaatca attggtttag
     8401 attatgacgc gccatatagt ggcttatgtg tagaaagttt cactggagat tatcctgcag
     8461 ggttatatga ttatgaagca aattataaag cgcatttaag tcatcgacaa aagcaatata
     8521 tttctaaaaa caaacacttt tttgatagcg agggaaattt aaatgtctaa agcatatgaa
     8581 caatctggtg taaatattca tgctggttat gaagctgtag aaagaatgtc tagtcatgtt
     8641 aaacgtacga tgcgtaaaga agttatcggt ggtttaggtg gattcggtgc tacatttgat
     8701 ttatcacaat taaatatgac agcgccagtt ttagtttctg gaacagacgg agtaggtacg
     8761 aaattaaaac tagctatcga ctatggtaaa catgattcga taggtatcga tgcagtcgca
     8821 atgtgtgtta atgatatttt aacgacaggt gcagaaccat tatatttttt agattacatt
     8881 gctacaaaca aagttgttcc tgaagttatt gaacaaattg ttaaaggtat tagtgatgca
     8941 tgtgttgaaa cgaatactgc acttatcggt ggagagactg ctgaaatggg tgaaatgtat
     9001 cacgaaggcg aatatgatgt agctggattt gcagttggag cagttgaaaa ggatgactat
     9061 gtagatggtt cagaagtgaa agagggacaa gttgttatag ggcttgcgtc aagtggcatt
     9121 cattcaaatg gatacagttt agtgcgcaaa ttaattaatg aatcaggcat tgatttggca
     9181 tcaaacttcg ataatcgtcc atttatcgat gtctttttag aaccaactaa attatatgtc
     9241 aaacctgtac ttgctttgaa aaaagaagtt tctattaagg caatgaatca tattactggt
     9301 ggaggttttt atgaaaatat tccacgtgca ttgccagccg gatatgctgc tagaattgat
     9361 actacatcat ttccaacacc aaaaatattt gattggttac aacaacaagg caatatagac
     9421 acaaatgaaa tgtataacat ttttaatatg ggtattggct atacggttat cgttgatgaa
     9481 aaagatgtat cacgcgcttt gaagatttta gcagaacaaa atgtggaagc ctatcaaatt
     9541 ggtcatattg tgaaaaatga gtcaactgca attgaattgt tgggggtata acaatggtta
     9601 aaattgcgat ttttgcatca ggttcaggaa gtaactttga aaatatagtt gagcatgttg
     9661 aatcaggaaa acttgaaaat attgaagtta cggcgctata tacggatcat caaaatgcgt
     9721 tttgtataga tagagcaaaa aagcacgata ttcctgttta tattaatgaa ccaaaacaat
     9781 ttgattcaaa agcagcgtat gaacaacatt tagtaacatt attaaatgaa gataaggtag
     9841 agtggattat tctagctggc tacatgcgtc taattggtcc agacttatta gcttcatttg
     9901 aaggtaaaat attgaatata catccatctc tattgccgaa atataagggg attgacgcaa
     9961 taggccaagc atatcatagt ggcgatacta ttactggttc gacagtacat tatgttgata
    10021 gtggtatgga tacgggagaa attattgaac agagaaaatg tgatattaga ccggacgatt
    10081 caaaagaaca attagaagag aaagtaaaaa aattggaata tgagttatat ccaagtgtta
    10141 ttgctaaaat tgtaaaataa ggagtatgta tgtgatgaag aaagctattt tgagcgtatc
    10201 aaataaaaca ggtattgtag agtttgctaa agcgttaacg caattaaatt atgaattata
    10261 ttcaacaggt ggtactaaac gtatattaga tgaagcaaat gtaccagttc gttctgtttc
    10321 agacttaaca cattttccag aaataatgga tggccgtgtt aaaacattac atccggctgt
    10381 tcatggtggc attttagctg atcgaaataa accgcagcat ttaaatgaat tatcagaaca
    10441 acatatagat ttaattgata tggtagtagt taatttatat ccattccaac aaactgttgc
    10501 aaaccctgat gtgacgatgg acgaagcaat tgaaaatatt gatattggtg gtccaacaat
    10561 gttacgtgct gcagctaaaa actataaaca tgtaacaaca attgtacatc cggcagatta
    10621 tcaagaagta ttgacgcgat taagaaacga ttcgttagat gagtcatata gacaatcatt
    10681 aatgattaaa gtttttgagc atactgcaga atatgatgaa gcgattgtac gtttctttaa
    10741 aggggataaa gaaactttaa gatatggaga aaatccacaa caatcagcgt attttgtgag
    10801 aacttcgaat gctaagcaca cgattgcagg cgctaaacaa ttacatggga aacaattaag
    10861 ctataacaat attaaagatg cagatgctac actagcttta gttaaaaagt ttgatacacc
    10921 tgctacagtt gcggttaaac acatgaatcc atgtggtgtt ggtatcggtg acacgataga
    10981 acaagcattt caacatgcgt atgaagcgga tagtcaatca atatttggtg gaattgttgc
    11041 attaaaccga gctgtaacac ctgagttagc agagcaattg catagtatct ttttggaagt
    11101 cattattgca ccaaaattta cagatgaagc attagatatt ttaaaacaaa agaagaatgt
    11161 aagattatta gaaattgata tgactataga cagtaacgaa gaagagtttg tttcagtatc
    11221 tggcggatat ttagttcaag ataaagacaa ttatgtcgtg ccaaaagaag aaatgaaagt
    11281 tgttacagaa gtagcaccta cagatgaaca atgggaagca atgttattag gatggaaagt
    11341 tgtaccatca gtaaaaagta atgcaattat tttaagtaat aataaacaaa ctgtaggtat
    11401 aggtgctgga caaatgaatc gtgtcggtgc tgctaaaatt gcgttagaga gagctattga
    11461 aatcaatgat catgtagcgt tagtatctga tggatttttc cctatgggag atacagttga
    11521 acttgcagca caacatggta taaaggcaat tatccaaccg ggtggttcga ttaaagatca
    11581 agattcaatt gatatggcta ataaacatgg tattgcaatg gtagtcacag gcactcgaca
    11641 ttttaaacac taattttaaa ggggcgtaac ataaatgaat gtattagtaa ttggtgctgg
    11701 tggacgagaa catgcacttg catataaact taatcaatcg aatctagtta aacaagtgtt
    11761 tgtcattcca ggtaatgagg caatgacacc tatagctgaa gtacacactg aaatttcaga
    11821 atctgatcat caagcgatac tagattttgc taaacggcaa aatgttgatt gggtagttat
    11881 aggtccagaa cagccgctaa ttgatggatt agcagacatt ttacgagcga atggtttcaa
    11941 agtgtttggt ccaaataagc aagcagctca aatcgaaggc tcaaaattat ttgctaaaaa
    12001 gataatggaa aaatataata ttccaactgc tgattataaa gaagttgagc gaaaaaagga
    12061 tgctttaaca tatattgaaa actgtgaatt gcccgttatt gtcaagaaag atgggttagc
    12121 tgctgggaaa ggcgttatta ttgcagatac tattgaagca gccagaagtg ctattgagat
    12181 tatgtatggt gatgaagaag aaggtactgt tgtatttgaa acgtttttag aaggtgaaga
    12241 gttctcgcta atgacatttg ttaatggtga tttagcagta cctttcgact gtattgcaca
    12301 agatcataaa cgcgcatttg atcatgatga aggaccaaat actggtggta tgggggctta
    12361 ttgtccagta ccacatatta gtgacgatgt tttaaaactt acaaatgaaa caattgcaca
    12421 acccattgca aaggcaatgc ttaatgaagg ttatcaattc ttcggtgtat tatacattgg
    12481 tgctatttta actaaagatg gtccaaaagt aatagaattt aatgcccgtt ttggtgatcc
    12541 tgaagctcaa gtattattaa gtcgcatgga aagtgattta atgcagcata ttattgattt
    12601 agatgaagga aaacgtactg aattcaaatg gaaaaatgaa tctattgtag gggtcatgtt
    12661 ggcatcaaaa ggatatcctg atgcatatga aaaagggcat aaagtaagtg gctttgattt
    12721 aaatgaaaac tattttgtta gtggattaaa gaagcaaggt gatacctttg ttacttcagg
    12781 tggtagagtt atacttgcca tcggaaaagg tgacaatgta caagatgcac agcgagacgc
    12841 atacaaaaaa gtatcacaaa tacaaagtga ccatttattc tatcgtcatg acattgcgaa
    12901 taaagcacta caacttaaat aagtaaattt aaaatactaa gattagctat gaacgaatct
    12961 ataacgatag attttttcat agctttttta gttgtagagt ctaggacatt gatttctgta
    13021 ccaaatttgt gattatgcat atgtaataca aaagaggcgc cacaacatgt ttggatgaac
    13081 aaaataacat gtttgtggca cctcttttgt ttagtatgga ataaatggtt ttctttttct
    13141 atacaatgaa tttctaattt agtatctata caattatgga taaaatttaa cctacacgac
    13201 caagacgaac atcatctatg cccgtgatgg gtaaggtgat tgaacaataa tatgccatag
    13261 taataatggc aattaaaact ataataaaga ttatatcttt atatgagaaa ggtacgttgt
    13321 aatagtaagt acgaggacca tctctaaatc ctttcgactc catcgcaact gataattgat
    13381 gtgcctttct aatattttgg cttaatagag gtataattaa atgcttaaat cgctttaacc
    13441 ctctataatt tgccgcgtct atcatctgat agcgcatttt taaagatctg cgaagctgta
    13501 ttaaagaact aatcattaaa ggtatcatac gaatggcagc catgaatgca taagcaactt
    13561 ttgatttaac ctttaaatgt tgcattaaac tataaaatat catgacaacc tgagatgtaa
    13621 gtgcgattaa gataccgaaa aatgaaatag caatggttct taatgataca tgtaaaccac
    13681 gaactaaact ttctgttgta atatggataa atccgaattt caaaattgta tggctaccat
    13741 tcccgtataa aatcatgaac agggaagaga gtaatgcaaa gccaatactt atagttataa
    13801 aaattgctgt aattttaaac tgagtaccat taaacatcaa taagaaaact aacattaaga
    13861 tagtgatata aagcataaaa tcgaaattat gcacaaatat aataaagaaa aatagtataa
    13921 ttccaagaaa tagtttcgtt ataatgttga catcatcaac aaatgattgc cgaactttcc
    13981 attgctcata cattcgtatc accatcacaa tctagtaacg caccatctga aattttaagt
    14041 cttcttgatg gataacgttc aattatttca tcgtcatgtg taaccatgac aatactttgt
    14101 cccaaattaa ttcgcttttg gaaaagtttg atcaactgga atgtattatg gctatcaagt
    14161 ccaaatgtcg gttcatctaa aaagataata tcagctttag aacttagtgc ggtagctacg
    14221 ctaaggcgtc gtttttgacc aatagacaac tcataaggat gttgatcttt tacattttgt
    14281 aaatctaaaa gttttaaaag ttgtatcgtt tcatcatcac tttgatcttt agaaaggtga
    14341 ttaaaatgaa tgttaatttc atcataaacc gaatttgtta taaattgtaa ttctgggttt
    14401 tgataaacta ggtacatgtg ttttgctgca tgtttaattt ttgttaaacg ctgattttca
    14461 aaataaacat caccttgata tttaatcaat tgcataattg attcaagcaa ggttgtttta
    14521 ccactaccat ttgcccctgt aattgtaatc cactcaccta gaccaatttc taaatctgag
    14581 aatgagagca atgttgattt accgcgaata atacgtccat ttttaaattg taataagtgt
    14641 gagtttgttg ttggaaagtc aacacgactt ggtgcgaatt cccatgcacg tggatgccac
    14701 acaccatatt cactgagtaa atgaacatac ttctgtaata tgatttcagg acattcatcg
    14761 gcaatgatat ttccgttata atccatcaaa atgacgcggt cgacatgatt ccagatgtgt
    14821 ttaactttat gttcaacgat tacaaccgtt tgatcttccc aaagttcaat tagtttagtc
    14881 cataaatctt ctgttgcttg aacatctaac attgctgtcg gttcatctaa aaacaatgtt
    14941 tttgattgtt gaagaatggt ttcaacaatt gccaatttct gtttcatccc gccacttaaa
    15001 tctttgatat acgtttcagg ggtaacattt aaattgacca tatttaaagc attgataatt
    15061 aacgcatcca tgtcttcacg tggtaattgt ctattttcta aaacgaatgc aagttcttcg
    15121 tatacttttg gcatacaaaa ctggctatca gggtcttgga aaataacgcc acttaatggg
    15181 tcaacgatta gttcatcata tttcataggt aattcaatta aattaggaac aataccactt
    15241 aatacattca gaagtgtact tttaccgcaa ccagaaggac cgagtaaaag tactttttct
    15301 ttgtcttgaa tagtgatatt taaatgatcg aaaattttac gttgaccact tggatatttt
    15361 aatcgtaaat cacttacttt taacactctg aatgctcctc ttataagttg tcgtaatctt
    15421 ctttagcagc tggtctaaat aattttgtta cgcctgtctt atctaaagct tttactaaaa
    15481 ggtaagatag gacgccggcg actactgcac cactaattaa tctaaatacg atgaataatg
    15541 ttaagttcca acctgcaact tcatttaaat aaccatagaa ataatctatc gggaaagccg
    15601 cgattgctgt acaaaaacct gctaacatag ctaccataac tgaacgtgat tgatatttaa
    15661 aaattgcaaa gacaagttca cacgctaaac cttgtataaa agcgtaaacg attgtcggaa
    15721 tatcgaaacg acccataatg atagtttcgc cggcacctgc agcaaattca gccagtaaag
    15781 caatacctgg ttttggaata attagatagc agacaatcgc tgccatgaac caaaccccgt
    15841 ttgttaattg ttcgaggtga aggcctgtag cttgcacacc attgtaaaca aaccaccata
    15901 aattgtaaat aactgcgaat actactgaaa taagtacggt tactagtatt tcagatagct
    15961 ttaaaccttt tgacattttt acatcctcct aataaaaaaa cgcacaacca tccataggaa
    16021 agttatgcgt tcacaatata tattagtaaa acatatgtat agtaacactt tcctacgcta
    16081 gttcaagcta gatcaggttc aaagggtttg agggcaagcc tcatctcagt ataaaacacc
    16141 cctagtgtgt gcgatttatt taattaatta tactgtaaga cgtttgtaaa cttatgtcaa
    16201 taggttgtct tcatgaaatt tcgtttaatt cgatttaaaa tttataatat tagcattgga
    16261 tttaaattga agatgtagta ggaatgttag taattaaaga tataaaaata tgtgacatgt
    16321 aataatattg agctgataaa tgaagaggga tacttatcaa tcatacctct ttaacaacag
    16381 tgaagaaccc gtgcataatg gcttacgaat tatagtttat aaggaagaag agggatacat
    16441 gcgccgagca catgcataaa agcccctaac aactaaaagt tgtaaggaag gagagggata
    16501 catgcgccga gcacatgcat aaaatcccct aacaactaaa agttgtaagg ggatttaaat
    16561 taatttagtg tatcttggat atcttgtttt gtttgattaa tatcttctgt tttttcttct
    16621 tttttatctt ttaatttttc ttcaacttct ttagcttttt ctgctgcttt tttattttga
    16681 ttttcattag acat
//
LOCUS       DAXWFK010000045        16614 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.045, whole genome shotgun sequence.
ACCESSION   DAXWFK010000045 DAXWFK010000000
VERSION     DAXWFK010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16614
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.045"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            61..1590
                     /gene="aur"
                     /locus_tag="ACGEXS_001147"
     CDS             61..1590
                     /gene="aur"
                     /locus_tag="ACGEXS_001147"
                     /EC_number="3.4.24.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloproteinase aureolysin"
                     /protein_id="HFF8012166.1"
                     /translation="MRKFSRYAFTSMAALTLLSTLSPAALAIDSKNKPANSDIKFEVT
                     QKSDAVKALKELPKSENVKNIYQDYAVTDVKTDKKGFTHYTLQPSVDGVHAPDKEVKV
                     HADKSGKVVLINGDTDAKKVKPTNKVTLSKDDAADKAFKAVKIDKNKAKNLKDKVIKE
                     NKVEIDGDSNKYVYNVELITVTPEISHWKVKIDAQTGEILEKMNLVKEAAETGKGKGV
                     LGDTKDININSIDGGFSLEDLTHQGKLSAFSFNDQTGQATLITNEDENFVKDEQRAGV
                     DANYYAKQTYDYYKDTFGRESYDNQGSPIVSLTHVNNYGGQDNRNNAAWIGDKMIYGD
                     GDGRTFTSLSGANDVVAHELTHGVTQETANLEYKDQSGALNESFSDVFGYFVDDEDFL
                     MGEDVYTPGKEGDALRSMSNPEQFGQPAHMKDYVFTEKDNGGVHTNSGIPNKAAYNVI
                     QAIGKSKSEQIYYRALTEYLTSNSNFKDCKDALYQAAKDLYDEQTAEQVYEAWNEVGV
                     E"
     gene            1957..2406
                     /locus_tag="ACGEXS_001148"
     CDS             1957..2406
                     /locus_tag="ACGEXS_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFF8012167.1"
                     /translation="MKKSKRLEIVSTIVKKHKIYKKEQIISYIEEYFGVRYSATTIAK
                     DLKELNIYRVPIDCETWIYKAINNQTEQEMREKFRHYCEHEVLSSIINGSYIIVKTSP
                     GFAQGINYFIDQLNIEEILGTVSGNDTTLILTASNDMAEYVYAKLFK"
     gene            2409..2576
                     /locus_tag="ACGEXS_001149"
     CDS             2409..2576
                     /locus_tag="ACGEXS_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012168.1"
                     /translation="MYQMNNKNLFRITCTQVSYQILTCVRVFLWKERIHSHSIDCIKN
                     FIQHVFMGIFE"
     gene            2753..3988
                     /gene="arcA"
                     /locus_tag="ACGEXS_001150"
     CDS             2753..3988
                     /gene="arcA"
                     /locus_tag="ACGEXS_001150"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFF8012169.1"
                     /translation="MTDGPIKVNSEIGALKTVLLKRPGKELENLVPDYLDGLLFDDIP
                     YLEVAQKEHDHFAQVLREEGVEVLYLEKLAAESIENPQVRSEFIDDVLAESKKTILGH
                     EEEIKALFATLSNQELVDKIMSGVRKEEINPKCTHLVEYMDDKYPFYLDPMPNLYFTR
                     DPQASIGHGITINRMFWRARRRESIFIQYIVKHHPRFKDANIPIWLDRDCPFNIEGGD
                     ELVLSKDVLAIGVSERTSAQAIEKLARRIFENPQATFKKVVAIEIPTSRTFMHLDTVF
                     TMIDYDKFTMHSAILKAEGNMNIFIIEYDDVNKDIAIKQSSHLKDTLEDVLGIDDIQF
                     IPTGNGDVIDGAREQWNDGSNTLCIRPGVVVTYDRNYVSNDLLRQKGIKVIEISGSEL
                     VRGRGGPRCMSQPLFREDI"
     gene            4021..5031
                     /gene="argF"
                     /locus_tag="ACGEXS_001151"
     CDS             4021..5031
                     /gene="argF"
                     /locus_tag="ACGEXS_001151"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFF8012170.1"
                     /translation="MTEIQKPYDLKGRSLLKESDFTKAEFEGLIDFAITLKEYKKNGI
                     KHHYLSGKNIALLFEKNSTRTRAAFTVASIDLGAHPEFLGKNDIQLGKKESVEDTAKV
                     LGRMFDGIEFRGFSQQAVEDLAKFSGVPVWNGLTDDWHPTQMLADFMTIKENFGYLEG
                     INLTYVGDGRNNIAHSLMVAGAMLGVNVRICTPKSLNPKEAYVDIAKEKASQYGGSVM
                     ITDNIAEAVENTDAIYTDVWVSMGEESEFEQRINLLKDYQVNQQMFDLTGKDSTIFLH
                     CLPAFHDTNTLYGQEIYEKYGLAEMEVTDQIFRSEHSKVFDQAENRMHTIKAVMAATL
                     GS"
     gene            5122..6552
                     /gene="arcD"
                     /locus_tag="ACGEXS_001152"
     CDS             5122..6552
                     /gene="arcD"
                     /locus_tag="ACGEXS_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFF8012171.1"
                     /translation="MNESGDNKLSKYSLIGLVIGSMIGGGAFNIMSDMGGKAGGLAII
                     IGWIITAIGMISLAFVFQNLTNERPELDGGIYSYAQAGFGDFVGFISAWGYWFSAFLG
                     NVAYATLLMSAVGNFFPIFKGGNTLPSVIVASLLLWGVHFLILKGVETAAFINSIVTV
                     AKLIPILLVIICMIIAFNFDTFKTGFFSMTSEGVLPFSWASTMSQVKSTMLVTVWVFI
                     GIEGAVIFSSRAKNKKDVGSATVIGLISVLIIYFLLTVLAQGVILQNHISQLDSPSMA
                     QVLATIVGGWGSTLVNIGLIISVLGAWLGWTLLAGELPFIVAKDGLFPKWFAKENKNG
                     APVNALLITNILVQLFLISMLFTQSAYQFAFSLASSAILYPYMFSAFYQVKYTLEHRQ
                     QATTKQWTIGIIASIYAIWLIYAAGINYLLLTMLLYIPALLVYTIVQKNNQTRLIKSD
                     YILFMIIIVLAVIGLIKLLMGTINVF"
     gene            6569..7510
                     /gene="arcC"
                     /locus_tag="ACGEXS_001153"
     CDS             6569..7510
                     /gene="arcC"
                     /locus_tag="ACGEXS_001153"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFF8012172.1"
                     /translation="MKEKIVIALGGNAIQTTEATAEAQQTAIRCAMQNLKPLFDSPAR
                     IVISHGNGPQIGSLLIQQAKSNSDTTPAMPLDTCGAMSQGMIGYWLETEINRILTEMN
                     SDRTVGTIVTRVEVDKDDPRFDNPTKPIGPFYTKEEVEELQKEQPDSVFKEDAGRGYR
                     KVVASPLPQSILEHQLIRTLADGKNIVIACGGGGIPVIKKENTYEGVEAVIDKDFASE
                     KLATLIEADTLMILTNVENVFINFNEPNQQQIDDIDVATLKKYAAQGKFVEGSMLPKI
                     EAAIRFVESGENKKVIITNLEQAYEALIGNKGTHIHM"
     gene            7609..8313
                     /locus_tag="ACGEXS_001154"
     CDS             7609..8313
                     /locus_tag="ACGEXS_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFF8012173.1"
                     /translation="MTENFILGRNNKLEHELKALADYINIPYSILQPYQSECFVRHYT
                     KGQVIYFSPQESSNIYFLIEGNIIREHYNQNGDVYRYFNKEQVLFPISNLFHPKEVNE
                     LCTALTDCTVLGLPRELMAFLCKANDDIFLTLFALINDNEQQHMNYNMALTSKFAKDR
                     IIKLICHLCQTVGYDQDEFYEIKQFLTIQLMSDMAGISRETAGHIIHELKDEKLVVKD
                     HKNWLVSKHLFNDVCV"
     gene            8663..11314
                     /gene="clfB"
                     /locus_tag="ACGEXS_001155"
     CDS             8663..11314
                     /gene="clfB"
                     /locus_tag="ACGEXS_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin clumping factor ClfB"
                     /protein_id="HFF8012174.1"
                     /translation="MKKRIDYLSNKQNKYSIRRFTVGTTSVIVGATILFGIGNHQAQA
                     SEQSNDTTQSSKNNASADSEKNNMIETPQLNTTANDTSDISANTNSANVDSTTKPMST
                     QTSNTTTTEPASTNETPQPTAIKNQATAAKMQDQTVPQEANSQVDNKTTNDANSIATN
                     SELKNSQTLDLPQSSPQTISNAQGTSKPSVRTRAVRSLAVAEPVVNAADAKGTNVNDK
                     VTASNFKLEKTTFDPNQSGNTFMAANFTVTDKVKSGDYFTAKLPDSLTGNGDVDYSNS
                     NNTMPIADIKSTNGDVVAKATYDILTKTYTFVFTDYVNNKENINGQFSLPLFTDRAKA
                     PKSGTYDANINIADEMFNNKITYNYSSPIAGIDKPNGANISSQIIGVDTASGQNTYKQ
                     TVFVNPKQRVLGNTWVYIKGYQDKIEESSGKVSATDTKLRIFEVNDTSKLSDSYYADP
                     NDSNLKEVTDQFKNRIYYEHPNVASIKFGDITKTYVVLVEGHYDNTGKNLKTQVIQEN
                     VDPVTNRDYSIFGWNNENVVRYGGGSADGDSAVNPKDPTPGPPVDPEPSPDPEPEPTP
                     DPEPSPDPEPEPSPDPDPDSDSDSDSGSDSDSGSDSDSESDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSESDSDSESDSESDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSRVTPPNNEQKAPSNPKGEVNHSNKVSKQHKTDALPETGDKSENTNA
                     TLFGAMMALLGSLLLFRKRKQDHKEKA"
     gene            11430..12191
                     /locus_tag="ACGEXS_001156"
     CDS             11430..12191
                     /locus_tag="ACGEXS_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFF8012175.1"
                     /translation="MAYISLNYHSPTIGMHQNLTVILPEDQSFFNSDTTVKPLKTLML
                     LHGLSSDETTYMRYTSIERYANEHKLAVIMPNVDHSAYANMAYGHSYYDYILEVYDYV
                     HQIFPLSKKRDDNFIAGHSMGGYGTIKFALTQGDKFAKAVPLSAVFEAQNLMDLEWND
                     FSKEAIIGNLSSVKGTEHDPYYLLDKAVAEDKQIPKLLIMCGKQDFLYQDNLDFIDYL
                     SRINVPYQFEDGPGDHDYAYWDQAIKRAITWMVND"
     gene            12436..12891
                     /locus_tag="ACGEXS_001157"
     CDS             12436..12891
                     /locus_tag="ACGEXS_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFF8012176.1"
                     /translation="MSTNKNDYEHMLFYFAYKTFITTADEIIEKYGMSRQHHRFLFFI
                     NKLPGITIKSLLEILEISKQGSHATLQKLKEQGLIIEKVLETDRRVKKLYSTDKGDQL
                     IAELNKAQDELLQNIYQQVGSDWYDVMEALAKGRPGFDFIKHLKDEKES"
     gene            13013..13114
                     /locus_tag="ACGEXS_001158"
     CDS             13013..13114
                     /locus_tag="ACGEXS_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016898541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SE2200 family small protein"
                     /protein_id="HFF8012177.1"
                     /translation="MKKLAVILTLVGGLYFAFKKYQERVNQAPNIEY"
     gene            complement(13174..14598)
                     /locus_tag="ACGEXS_001159"
     CDS             complement(13174..14598)
                     /locus_tag="ACGEXS_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFF8012178.1"
                     /translation="MLSVKKATKFAVANLIIVSTVFSSVTPAIAQSDKSSKDESFAVG
                     NTKNPKNVIFLVGDGMGPSFNTAYRYYKNDPHAKEMTPTAFDSYLKGTNRTYSNDPKQ
                     NITDSAAGGTAFSSGHKTYNGAIGVDSNKQKVKTVLERAKEKGKSTGLVSTAELTDAT
                     PAAYAAHVTSRDDKNEIAKQFYKDKINGKHKVDVLLGGGAKYFGKSNGNLDKKFKKDG
                     YDLATNSKELSKSDKDKVLGLFADKNMPLAIDTSKDEPSLADMQQSALSKLERNKKGF
                     FLMVEGASIDKSAHSNDITGVMSEMEGFEKAFDDAIQYAKKHKDTLVVATADHSTGGL
                     TIGKDKGYEWNPQPIKSMKHSGSYMTEKMAKGEDPEKVINEGYGFEFSQDDMKKVKKE
                     NKKLKRLLKKEKDEKSAEVETQTKALQHAIQKPVNDKSYTGWTSDGHTGEDVNTYAYG
                     PRSESFSGNIENTESAKIIFDIFK"
     gene            14796..14996
                     /locus_tag="ACGEXS_001160"
     CDS             14796..14996
                     /locus_tag="ACGEXS_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012179.1"
                     /translation="MILVMLSPLLIIFFIVLSILEERKRTKKKQLEKEKVNTLNQNTN
                     DTESSNQEPSLQQDKEQKDNKG"
     gene            15022..15687
                     /gene="nsaR"
                     /locus_tag="ACGEXS_001161"
     CDS             15022..15687
                     /gene="nsaR"
                     /locus_tag="ACGEXS_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nisin susceptibility-associated two-component
                     system response regulator NsaR"
                     /protein_id="HFF8012180.1"
                     /translation="MKILIVEDDFVIAESLASELKKWNYGVIVVEQFDDILSIFNQNQ
                     PQLVLLDINLPTLNGFHWCQEIRKTSNVPIIFISSRIDNMDQIMAIQMGGDDFIEKPF
                     NLSLTIAKIQALLRRTYDLSVANDSLTVKGCTLILDEAKVVYQEQNIQLSLTELQILK
                     LLFQNEDKYVSRTALIEKCWESENFIDDNTLAVNMTRLRKKLNTIGVNDFIITKKNVG
                     YKV"
     gene            15698..16585
                     /gene="nsaS"
                     /locus_tag="ACGEXS_001162"
     CDS             15698..16585
                     /gene="nsaS"
                     /locus_tag="ACGEXS_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nisin susceptibility-associated two-component
                     system sensor histidine kinase NsaS"
                     /protein_id="HFF8012181.1"
                     /translation="MTFLKSITQEIAIVIVIFALFGLMFYLYHLPLEAYLLALGVILL
                     LLLIFIGIKYLSFVKTISQQQQIENLENALYQLKNEQIEYKNDVESYFLTWVHQMKTP
                     ITAAQLLLERDEPNVVNRVRQEVIQIDNYTSLALSYLKLLNETSDISVTKISINNIIR
                     PIIMKYSIQFIDQKTKIHYEPCHHEVLTDVRWTSLMIEQLINNALKYARGKDIWIEFD
                     EQSNQLHVKDNGIGISEADLPKIFDKGYSGYNGQRQSNSSGIGLFIVKQISTHTNHPV
                     SVVSKQNEGTTFTIQFPDE"
BASE COUNT         6006 a         2479 c         3080 g         5049 t
ORIGIN      
        1 gaaaaattaa attaatattt tatttataag ctgtttttaa gatttcagga ggaatgaaat
       61 gtgaggaaat tttcaagata tgcatttaca agtatggcag cattaacctt gttgagcact
      121 ttatcaccag cagcattagc gattgattca aaaaataaac cagctaattc tgatattaaa
      181 tttgaggtga ctcaaaagag tgatgcggtc aaagcattaa aagaattgcc taaatccgaa
      241 aatgtaaaaa atatttatca agattacgct gttactgatg taaaaactga taaaaaagga
      301 tttacgcatt atacattgca accgagtgtt gatggtgttc atgcacctga caaagaagtg
      361 aaagtacacg cagacaaatc aggaaaagtc gttttaatca atggggatac tgatgcgaag
      421 aaagtaaagc caacgaataa agtgacatta agtaaagatg acgcagccga caaagcattt
      481 aaagcagtta agattgataa gaataaagcg aaaaatctta aagataaagt cattaaagaa
      541 aacaaagttg aaatcgatgg tgacagtaat aaatacgttt ataatgttga gttaattaca
      601 gtgacaccag aaatttcaca ttggaaagtt aaaattgatg ctcaaactgg cgaaatttta
      661 gaaaaaatga acttagttaa agaagctgca gaaactggta aaggaaaagg tgtacttggc
      721 gatacaaaag atatcaatat caatagtatt gacggtggat ttagcctaga agatttaacg
      781 catcaaggta aattatcagc atttagcttt aatgatcaaa caggtcaagc aacattgatt
      841 actaatgaag atgaaaactt cgtaaaagat gagcaacgtg ctggcgtaga tgcaaattat
      901 tacgctaaac aaacatatga ttattacaaa gacacatttg gtcgtgaatc atatgacaac
      961 caaggtagtc caattgtttc attaacgcat gttaataact acggtggtca agataacaga
     1021 aataatgccg catggatcgg tgacaaaatg atctatggtg atggtgatgg tcgcacattc
     1081 acaagtttat cgggtgcaaa tgacgtagta gcacacgaat taacacacgg tgtgacacaa
     1141 gagacagcga acttagaata taaggaccag tcaggcgctc taaatgaaag cttttcagat
     1201 gtttttggat actttgtaga tgacgaggat ttcttaatgg gtgaagatgt ctacacacct
     1261 ggaaaagagg gagacgcttt acgcagcatg tcaaacccag aacaatttgg tcaaccagct
     1321 catatgaaag actatgtatt cactgaaaaa gataatggtg gcgtacatac gaattctgga
     1381 attccaaata aagcagctta taacgtgatt caagcaatag ggaaatctaa atcagaacaa
     1441 atttactacc gagcattaac ggaatactta acaagtaatt caaacttcaa agattgtaaa
     1501 gatgcattat accaagcggc taaagattta tatgacgagc aaacagctga acaggtgtat
     1561 gaagcatgga atgaagtagg cgtggagtaa aaatatataa acaagaagaa gtaatgttaa
     1621 acacttataa ataattaaat tttaaataca gcttatatat ggggtatacc tacattgata
     1681 attgactttg taggtatacc ctctctttgt aattagatga gaggagtgtg agggttgtct
     1741 gccgaaagac tactcggcag tctaaaatca ttacaagtag tagatatgtg ataattaaat
     1801 gctgacttag aatacaaaat tcattttaaa agttgtcaca aaaaatttac atgtattttt
     1861 attatctttt gcaaaacaaa gtgttaaatt ataaatgaaa catgcatgaa tttatttttt
     1921 aatacaagaa acgtaactac caaaggagtt tacaatatga agaaaagtaa acgattagaa
     1981 attgtttcta caatagttaa aaagcataag atttataaaa aagaacaaat catttcatat
     2041 attgaagaat attttggtgt aagatatagt gcaacaacaa ttgctaaaga cttgaaggaa
     2101 ctaaatatat atcgtgtccc tatcgattgt gagacatgga tttataaagc tattaataat
     2161 caaacagaac aagagatgag agaaaagttt agacactatt gtgaacatga agttctaagt
     2221 tcaatcatca atggttcata cattatcgtc aaaacctcac ctggtttcgc ccaaggcata
     2281 aactatttta tcgatcagct aaatatagaa gagatattag gtacggtgag tggaaatgac
     2341 actacattaa tcttaactgc ctcaaatgat atggcagaat acgtatatgc aaaattattt
     2401 aaatagacat gtatcaaatg aataataaaa atttgtttcg tatcacgtgt actcaagtta
     2461 gttaccaaat attaacttgt gtacgcgttt ttttatggaa agaaagaatt catagtcatt
     2521 caattgactg tataaaaaac tttatacaac atgtttttat gggtattttt gaataaaaaa
     2581 tgtatatttc gacccaaaat acctttattt atgtataaaa atccattatt atgtattgta
     2641 taacaaaaag atatgaaatt ttcgactttc tttatgtgaa tataatcaca tgtaagcgtt
     2701 tgaagattgt ctatactcta aatgaattca aagataaaag gaggaaatag acatgacaga
     2761 tggtccaatt aaagtaaata gcgaaattgg agctttaaaa actgtgttac ttaagcgtcc
     2821 tggaaaagaa ttagaaaatt tagtacctga ttatttagat ggattactat ttgatgatat
     2881 tccatattta gaagtagctc aaaaagagca tgaccatttt gcgcaggtgc taagagaaga
     2941 gggtgttgaa gtactttacc ttgagaagtt agcagctgaa agtattgaaa atcctcaagt
     3001 aagaagtgaa tttattgatg atgtattagc agagtctaaa aaaacaatat taggtcatga
     3061 agaagaaatt aaggcattat ttgcgacact ttctaatcaa gaacttgtag ataaaataat
     3121 gtcaggggta cgtaaggaag aaattaatcc gaaatgtaca catctagtag agtatatgga
     3181 tgataagtat ccattctatt tagatccaat gccaaacctt tattttacta gagatccaca
     3241 agcctcaata ggacacggta taacaatcaa tcggatgttc tggagagcac gacgacgaga
     3301 atcaatattt attcaatata ttgtaaagca tcatcctaga tttaaagatg cgaatattcc
     3361 aatctggtta gatcgagatt gcccattcaa tattgaaggc ggcgatgaac ttgttttatc
     3421 taaagatgtc ttggctatag gcgtttcaga acgtacatct gcacaagcta ttgaaaagtt
     3481 agcgcgacgt atttttgaaa atccgcaggc gacgtttaaa aaagtagtag caattgaaat
     3541 tccaactagt cgaactttta tgcacttaga tacagtattt acaatgatag attatgacaa
     3601 atttacaatg cattcagcca ttttaaaggc agaaggcaat atgaatatat ttattattga
     3661 atatgatgac gtaaataaag atattgccat caaacaatct agtcatttaa aagatacttt
     3721 agaagacgta ctaggtatag atgatatcca attcattcca acaggaaatg gtgatgtcat
     3781 tgatggtgct agagagcaat ggaatgatgg ctcaaataca ttatgtataa gaccaggcgt
     3841 tgtagtgact tacgatagaa actatgtatc gaatgattta ttgagacaaa aaggcattaa
     3901 agtcattgaa atatctggta gcgagttggt acgtggacgt gggggcccta gatgtatgag
     3961 tcaaccatta ttcagagaag acatttaacg taataaatta tagaaacgag ggtgaaaata
     4021 atgacagaaa ttcaaaaacc gtatgattta aaaggcagat cattattaaa agaaagtgat
     4081 tttaccaaag cagaattcga aggacttatt gattttgcaa ttacattaaa agagtataag
     4141 aaaaacggta ttaagcatca ctacttatct ggaaaaaata ttgcactact attcgaaaag
     4201 aattcgacga gaacgcgtgc tgcgtttaca gttgcgtcta ttgatttagg tgcgcatcca
     4261 gaatttttag gaaaaaatga tattcaatta ggcaaaaaag aatctgtaga ggatactgcg
     4321 aaagtattag gtagaatgtt cgatggtatt gaattccgtg gtttttcaca acaagctgtt
     4381 gaagatttag cgaagttctc tggtgtaccg gtgtggaatg gattaacaga cgattggcat
     4441 cctacacaaa tgttagctga ttttatgaca ataaaagaga attttggata tctagaagga
     4501 ataaacttaa cttacgttgg agatggacgt aataatattg cgcattcatt aatggtagca
     4561 ggtgctatgt taggtgttaa tgtaagaatt tgtacaccta aatcattaaa tccaaaagag
     4621 gcatatgttg atattgcaaa agaaaaagcg agtcaatatg gtggttcagt catgattacg
     4681 gataatattg cagaagcagt tgaaaataca gatgctatat atacagatgt ttgggtatcg
     4741 atgggtgaag aaagtgaatt tgaacaacgt attaatttat taaaagacta tcaagtgaat
     4801 caacagatgt ttgatttaac aggtaaagat tcaacgatat tcttacattg tttaccagca
     4861 ttccatgata caaatacact ttatggacaa gaaatttatg aaaaatatgg attagctgaa
     4921 atggaagtta cagaccaaat ctttagaagt gaacattcaa aagtgtttga tcaagctgaa
     4981 aatagaatgc atacaattaa ggcagtaatg gcagcaacat tggggagtta atcactaaat
     5041 ggaacgatat gaatatgatg tgtctgatga tataagtgtc atgtacagac acctcatatt
     5101 ggtattaaag gagaaatgaa tatgaacgaa tcaggagata acaaactcag taaatattct
     5161 ttaattggac tagttatagg atccatgatt ggtggcggtg cgttcaatat aatgtctgat
     5221 atgggcggta aagccggtgg attagccatt attattggtt ggattattac agctatagga
     5281 atgatttcat tagcgttcgt atttcaaaat ttaaccaatg aacggccgga gctagacggt
     5341 ggtatttata gttatgctca agcaggattt ggcgattttg taggatttat cagtgcttgg
     5401 ggatattggt tctcagcgtt tttaggcaat gttgcctatg caacactatt gatgtcagca
     5461 gtaggtaact ttttcccgat ttttaaagga ggcaacacat taccaagtgt tattgtcgcc
     5521 tcgttactac tctggggtgt ccatttcttg attttaaaag gcgttgaaac agcagcattt
     5581 atcaatagta ttgttactgt tgcaaagtta ataccgattt tacttgtaat catatgcatg
     5641 ataattgcat tcaattttga cacttttaaa acaggctttt tcagtatgac gtcagagggt
     5701 gtattgccat ttagttgggc gagcacaatg agccaagtta aaagtacgat gctagtgaca
     5761 gtttgggtgt ttatcggtat cgaaggtgca gtaatttttt ctagtagagc taaaaataaa
     5821 aaagatgtag gtagtgccac ggttatagga cttatatcag ttttaattat ctatttctta
     5881 ttaactgtat tagctcaagg cgtgattttg caaaatcata tttcgcaatt agattcgcca
     5941 agtatggcac aggtgcttgc aactattgta ggtggttggg gatctacact tgtaaatatt
     6001 ggtttaatta tttcggtact aggtgcatgg ttaggatgga cactgcttgc tggtgaatta
     6061 cctttcattg ttgcaaaaga tggattattt ccaaaatggt ttgctaaaga aaataaaaat
     6121 ggagcacctg taaatgcact gcttattacc aatatattag tacaattatt tttaataagt
     6181 atgctattta cacagagtgc gtatcaattt gcattttcac tagcatcaag tgctatttta
     6241 tacccttaca tgttcagtgc attttaccaa gttaaataca ctttagagca tcgacagcaa
     6301 gcaactacta aacaatggac gattggtatc atagcctcaa tttatgctat atggcttata
     6361 tatgcagcag gtatcaatta cttattattg acgatgttac tttatattcc agctcttctt
     6421 gtttatacaa tcgttcaaaa gaataatcag acacgtttga ttaaatcaga ctatattctt
     6481 tttatgatta ttatcgtact tgcagttatc gggttaatta agttattgat gggaacgata
     6541 aatgtttttt aaaaggagcg acaaaaatat gaaagagaaa attgtcattg cattaggcgg
     6601 taatgcgata cagacaacag aagcaacagc tgaagcacaa caaacagcta ttagatgtgc
     6661 gatgcaaaac cttaaacctt tatttgattc accagcgcgt attgtcattt cacatggtaa
     6721 tggtccacaa attggaagtt tattaatcca acaagctaaa tcgaacagtg acacaacgcc
     6781 ggcaatgcca ttggatactt gtggtgcaat gtcacagggt atgataggct attggttgga
     6841 aactgaaatc aatcgcattt taactgaaat gaatagtgat agaactgtag gcacaatcgt
     6901 tacacgtgtg gaagtagata aagatgatcc acgatttgat aacccaacta aaccaattgg
     6961 tcctttttat acgaaagaag aagttgaaga attacaaaaa gaacagccag actcagtctt
     7021 taaagaagat gcaggacgtg gttatagaaa agtagttgcg tcaccactac ctcaatctat
     7081 actagaacac cagttaattc gaactttagc agacggtaaa aatattgtca ttgcatgcgg
     7141 tggtggcggt attccagtta taaaaaaaga aaatacctat gaaggtgttg aagcggttat
     7201 agataaagat tttgctagtg agaaattagc aacgctgatt gaagcagata ccttaatgat
     7261 tcttacgaat gtagaaaatg tatttattaa ctttaatgaa cctaatcaac aacaaatcga
     7321 tgatattgat gtagcaacac tgaaaaaata cgcggcacaa ggtaagtttg tggaaggatc
     7381 gatgttgcca aaaatagaag ctgcgatacg atttgttgaa agtggggaaa acaaaaaagt
     7441 tatcattacc aatttagagc aggcatacga agctttgatt ggtaataaag gtacacacat
     7501 tcacatgtag cactttatca cgcgacaaaa cattaaatat gtttctccgt tgattcaaat
     7561 gaaaaagttg tctgctgaca ctttgcaagg tttgaaggag tttaacttat gacagaaaac
     7621 tttattttgg gtagaaataa taaattagaa catgaactaa aggcattagc agattacatt
     7681 aatataccat atagtatatt acaaccatat caaagtgaat gttttgtcag acattatacg
     7741 aaaggccaag ttatttattt ttcgccacaa gaaagtagca atatttactt tttaattgaa
     7801 ggtaacatta ttagagaaca ttacaatcaa aatggagatg tatatcgtta ttttaataaa
     7861 gagcaagtat tatttccaat cagtaactta tttcatccga aagaggttaa cgaattgtgt
     7921 acagcattaa ccgattgtac agttcttgga ttgcctagag aattgatggc ctttttgtgc
     7981 aaagctaatg atgatatatt tttgacactt tttgcattaa taaatgataa tgagcagcaa
     8041 cacatgaact ataacatggc attaacaagt aaatttgcta aagatcgaat tatcaaattg
     8101 atatgccatc tatgtcagac agtaggatac gatcaagatg aattttatga aatcaaacag
     8161 tttttaacta ttcaactcat gagtgatatg gctgggattt cccgggaaac agctggtcat
     8221 attattcatg aacttaaaga tgaaaaactt gttgttaaag atcataaaaa ttggttagta
     8281 agcaaacatt tattcaatga tgtatgtgtt taatatacaa tgtaaaatga ataagttgaa
     8341 catgaggtct aacgtacatt tatacgttag gccttttttg ctagcatgat gaataattta
     8401 aaatgttagt taaatttgat tgttgaaatt acagtaaaat ttaaggtgat gaaaaattta
     8461 gaacttctaa gtttttgaaa agtaaaaaat ttgtaatagt gtaaaaatag tatattgatt
     8521 tttgctagtt aacagaaaat tttaagttat ataaatagga agaaaacaaa ttttacgtaa
     8581 tttttttcga aaagcaattg atataattct tatttcatta tacaatttag actaatctag
     8641 aaattgaaat ggagtaatat ttttgaaaaa aagaattgat tatttgtcga ataagcagaa
     8701 taagtattcg attagacgtt ttacagtagg taccacatca gtaatagtag gggcaactat
     8761 actatttggg ataggcaatc atcaagcaca agcttcagaa caatcgaacg atacaacgca
     8821 atcttcgaaa aataatgcaa gtgcagattc cgaaaaaaac aatatgatag aaacacctca
     8881 attaaataca acggctaatg atacatctga tattagtgca aacacaaaca gtgcgaatgt
     8941 agatagcaca acaaaaccaa tgtctacaca aacgagcaat accactacaa cagagccagc
     9001 ttcaacaaat gaaacacctc aaccgacggc aattaaaaat caagcaactg ctgcaaaaat
     9061 gcaagatcaa actgttcctc aagaagcaaa ttctcaagta gataataaaa caacgaatga
     9121 tgctaatagc atagcaacaa acagtgagct taaaaattct caaacattag atttaccaca
     9181 atcatcacca caaacgattt ccaatgcgca aggaactagt aaaccaagtg ttagaacgag
     9241 agctgtacgt agtttagctg ttgctgaacc ggtagtaaat gctgctgatg ctaaaggtac
     9301 aaatgtaaat gataaagtta cggcaagtaa tttcaagtta gaaaagacta catttgaccc
     9361 taatcaaagt ggtaacacat ttatggcggc aaattttaca gtgacagata aagtgaaatc
     9421 aggggattat tttacagcga agttaccaga tagtttaact ggtaatggag acgtggatta
     9481 ttctaattca aataatacga tgccaattgc agacattaaa agtacgaatg gcgatgttgt
     9541 agctaaagca acatatgata tcttgactaa gacgtataca tttgtcttta cagattatgt
     9601 aaataataaa gaaaatatta acggacaatt ttcattacct ttatttacag accgagcaaa
     9661 ggcacctaaa tcaggaacat atgatgcgaa tattaatatt gcggatgaaa tgtttaataa
     9721 taaaattact tataactata gttcgccaat tgcaggaatt gataaaccaa atggcgcgaa
     9781 catttcttct caaattattg gtgtagatac agcttcaggt caaaacacat acaagcaaac
     9841 agtatttgtt aaccctaagc aacgagtttt aggtaatacg tgggtgtata ttaaaggcta
     9901 ccaagataaa atcgaagaaa gtagcggtaa agtaagtgct acagatacaa aactgagaat
     9961 ttttgaagtg aatgatacat ctaaattatc agatagctac tatgcagatc caaatgactc
    10021 taaccttaaa gaagtaacag accaatttaa aaatagaatc tattatgagc atccaaatgt
    10081 agctagtatt aaatttggtg atattactaa aacatatgta gtattagtag aagggcatta
    10141 cgacaataca ggtaagaact taaaaactca ggttattcaa gaaaatgttg atcctgtaac
    10201 aaatagagac tacagtattt tcggttggaa taatgagaat gttgtacgtt atggtggtgg
    10261 aagtgctgat ggtgattcag cagtaaatcc gaaagaccca actccagggc cgccggttga
    10321 cccagaacca agtccagacc cagaaccaga accaacgcca gatccagaac caagtccaga
    10381 cccagaaccg gaaccaagcc cagacccgga tccggattcg gattcagaca gtgactcagg
    10441 ctcagacagc gactcaggtt cagatagcga ctcagaatca gatagcgatt cggattcaga
    10501 cagtgattca gattcagata gcgattcgga ttcagacagt gattcagatt cagacagcga
    10561 ctcagaatca gatagcgact cagaatcaga tagtgagtca gattcagaca gtgactcgga
    10621 ctcagacagt gattcagact cagatagcga ttcagactca gatagcgatt cagactcaga
    10681 cagcgattca gattcagaca gcgactcaga ttcagacagc gactcagact cagatagcga
    10741 ctcagactca gacagcgact cagattcaga tagcgattca gactcagaca gcgactcaga
    10801 ctcagacagc gactcagact cagatagcga ctcagattca gatagcgatt cagactcaga
    10861 cagcgactca gattcagata gcgattcgga ctcagacagc gattcagatt cagacagcga
    10921 ctcagactcg gatagcgatt cagattcaga tagcgattcg gattcagaca gtgattcaga
    10981 ttcagacagc gactcagact cggatagcga ctcagactca gacagcgatt cagactcgga
    11041 tagcgactcg gattcagata gcgactcaga ctcagatagt gactccgatt caagagttac
    11101 accaccaaat aatgaacaga aagcaccatc aaatcctaaa ggtgaagtaa accattctaa
    11161 taaggtatca aaacaacaca aaactgatgc tttaccagaa acaggagata agagcgaaaa
    11221 cacaaatgca actttatttg gtgcaatgat ggcattatta ggatcattac tattgtttag
    11281 aaaacgcaag caagatcata aagaaaaagc gtaaatactt ttttaggccg aatacatttg
    11341 tattcggttt ttttgttgaa aatgatttta aagtgaattg attaagcgta aaatgttgat
    11401 aaagtagaat tagaaagggg tcatgacgta tggcttatat ttcattaaac tatcattcac
    11461 caacaattgg tatgcatcaa aatttgacag tcattttacc ggaagatcaa agcttcttta
    11521 atagcgatac aactgttaaa ccattaaaaa ctttaatgtt gttacatgga ttatcaagtg
    11581 atgaaacgac atatatgaga tatacaagca tagaaaggta tgcgaatgaa cacaaattag
    11641 ctgtgattat gcccaatgtg gatcatagcg catatgctaa catggcatat ggtcatagct
    11701 attatgatta tattttggaa gtgtatgatt atgttcatca aatatttcca ctttccaaaa
    11761 agcgtgatga caattttata gcaggtcact ctatgggagg atatggcaca attaaatttg
    11821 cattaacaca aggggataaa tttgccaaag ctgtaccatt atctgctgtg tttgaagcgc
    11881 aaaatttaat ggatctagag tggaatgatt tttcaaaaga ggccataatt ggcaatcttt
    11941 caagtgttaa aggaactgaa catgatccgt attacttgct agacaaagct gtagctgaag
    12001 ataaacaaat tccaaaattg ctcattatgt gtggtaaaca agacttttta tatcaagaca
    12061 acttagattt tatcgattat ttatcacgca taaatgttcc ttatcaattt gaagatggac
    12121 caggagatca tgattatgca tattgggatc aagcgattaa gcgtgctata acatggatgg
    12181 tgaatgatta attatttctt ggaaaatatg tggctgcatt aaatacacag agtgagagat
    12241 acaaactatt tacgcacgac taacatttct aagtgtttaa attatttttg tattaatatg
    12301 attggcgcaa tttgctgata cacaaaaatg tttctcgtga aacttagatt tagcttatag
    12361 ttttatcatc atttgtatga cttacattat aaattttatt ataatgaggt taacgctttg
    12421 aaaggagtca tcatcatgtc gaccaataaa aacgattatg agcatatgtt gttttatttt
    12481 gcatataaaa cctttattac taccgctgat gaaattatag agaagtatgg tatgagtcgt
    12541 cagcatcatc gttttttgtt ttttatcaat aaattacctg gtattactat taaatcatta
    12601 ctagaaatat tagaaatttc taaacaagga tcacatgcaa cacttcaaaa attaaaagag
    12661 caaggtctca ttattgaaaa agttttagag actgatcgac gtgtcaaaaa attatattcg
    12721 acggataaag gcgatcaact cattgctgaa ttgaacaagg cgcaagatga attattgcaa
    12781 aatatatatc aacaagtcgg ttcggattgg tatgatgtga tggaagcatt ggctaaaggg
    12841 cgacctggct ttgattttat taagcatttg aaagatgaaa aagaaagcta gcatcagaaa
    12901 tgttaaaaat cttcgcattc ttaaatttaa aaaatatgtc aaaaagtgta taataaaaac
    12961 atataattta attgaactca gtttcaacac atcttagaaa ggagtttgaa tgatgaaaaa
    13021 attagcagtt attttaacat tagttggcgg tttatacttc gcatttaaaa aataccaaga
    13081 acgtgttaac caagcaccta acattgagta ctaaattaaa ccataaaaaa ttcccgaaca
    13141 ccttgttata gtgctcggga atttttttat gctttacttg aatatatcaa atattatttt
    13201 tgcgctttct gtattttcga tattaccact aaatgattct gatctaggtc cgtaagcgta
    13261 ggtattaaca tcctcgcctg tatgtccatc ggaagtccac cctgtataag atttatcatt
    13321 tactggcttc tgaatagcgt gttgtagggc ttttgtttgc gtttctactt ctgcggattt
    13381 ttcgtctttt tcttttttaa gtagtctttt tagcttttta ttctcttttt taaccttttt
    13441 catatcatct tgtgaaaatt caaatccata accttcatta ataacttttt cagggtcttc
    13501 acctttagcc attttttctg tcatatatga tccagagtgt ttcatagatt taatcggttg
    13561 aggattccat tcgtatcctt tatctttacc aattgttaga ccaccagtag agtgatctgc
    13621 tgttgcaacg acaagtgtat ctttatgttt cttagcatat tgaatggcgt catcgaatgc
    13681 tttttcaaaa ccttccattt cagacataac gcctgtaata tcgttggaat gcgctgattt
    13741 atctatagaa gcaccttcga ccattaaaaa gaatcctttt ttattgcgct caagcttact
    13801 aagtgcactt tgttgcatat cagctaatga tggttcgtct ttagaagtat ctattgcaag
    13861 tggcatattt ttatctgcaa acaaaccaag aactttatct ttatcagatt ttgataactc
    13921 cttactgttc gtggcaaggt cgtaaccatc ttttttgaat tttttatcta aattgccatt
    13981 acttttaccg aaatatttag cgccgccgcc taataaaaca tcaactttat gctttccgtt
    14041 gattttatct ttataaaatt gtttagcgat ttcgttttta tcatctctag aagtcacgtg
    14101 tgcagcatat gctgctggtg ttgcatctgt taattcagct gttgaaacaa gaccagtcga
    14161 cttacctttt tcttttgcac gttcaagcac cgtctttact ttctgcttgt tactgtcaac
    14221 accgatggca ccattatatg tcttatgacc agaactaaag gctgttccgc cagctgcaga
    14281 atcagtaata ttctgttttg ggtcatttga atatgtacga tttgtgcctt ttaaatatga
    14341 atcaaaagca gtaggggtca tttctttagc atgcggatca tttttataat aacgataagc
    14401 tgtgttaaat gatggaccca tgccatcgcc aactaaaaag ataacatttt ttggattttt
    14461 agtattacca accgcgaaac tttcatcttt agaactttta tcggattgcg caattgcagg
    14521 tgtgacagaa ctaaaaaccg ttgacacgat aataaggtta gcaactgcaa attttgtggc
    14581 ttttttaact gataacataa gacatcctcc tgagtatatg actatgtctt cagtgtaaaa
    14641 gaggaatttt gagcaattat gtagttttag ttaaaaatat gtaaacagag tgatttagaa
    14701 taacaaaaaa tgaatatata tgacaatttg ttatagaaag cgttagaata gaagcgtgtg
    14761 aaaatataga attaaatata atttgaggtg gaaaaatgat actagtaatg ttatctccat
    14821 tattaatcat attctttata gtgttgtcta ttttagaaga gcgtaaacgt acgaagaaaa
    14881 agcaactcga gaaagaaaaa gtaaatacac taaatcaaaa tacaaatgac acggaaagtt
    14941 caaatcaaga gccgtcattg cagcaggata aagaacaaaa agataacaaa ggataattca
    15001 attgaaggaa gaagattata gatgaaaata ttaattgttg aagatgattt tgttatagca
    15061 gagagtttag catctgaact taaaaaatgg aattacggtg ttattgtcgt tgaacaattt
    15121 gatgatatac tgtctatctt taaccaaaat caacctcagc ttgtattgct agatattaat
    15181 ttgccaacgt taaatggttt tcattggtgt caagaaatcc gaaaaacatc taatgtgcca
    15241 attatattta ttagttcccg tattgataat atggaccaaa ttatggcaat acaaatgggg
    15301 ggagatgatt ttatcgaaaa gccatttaac ttgtcattaa cgattgccaa aattcaagca
    15361 ttattgagac gaacttatga cttgtcagta gctaatgatt cattgacggt taaaggttgt
    15421 acattaatat tagatgaagc aaaagtcgtg tatcaagaac aaaacataca gctatctttg
    15481 actgaattac aaatattaaa gttattattt caaaatgaag ataaatatgt aagtagaact
    15541 gctttaattg aaaaatgttg ggaatcagaa aacttcatag atgataacac attagctgtt
    15601 aacatgacgc gcctgcgtaa aaaattaaat actattggcg ttaatgattt tatcattaca
    15661 aagaaaaatg tcggatataa agtatagggt gaatgcaatg acctttctta aaagtattac
    15721 tcaggaaata gcaatagtca tagttatttt tgctttgttt ggcttaatgt tttacctgta
    15781 tcatttgcca ttagaagcat atttactagc acttggcgtt attttattat tattactcat
    15841 attcataggt attaaatatt taagttttgt aaaaactata agccaacaac aacaaattga
    15901 aaacttagaa aatgcgttgt atcagcttaa aaatgaacaa attgaatata aaaatgatgt
    15961 agagagctac tttttaacat gggtacatca aatgaaaaca cccattactg cagcacaact
    16021 gttacttgaa agagatgagc ctaatgttgt taatcgtgtt cgtcaagagg ttattcaaat
    16081 tgataactat acaagtttag cacttagtta tttaaagtta ttaaatgaaa cttctgatat
    16141 ttctgtcact aaaatttcga ttaataatat cattcgccca attattatga aatattcaat
    16201 acagtttatt gatcaaaaaa caaaaatcca ttatgaacct tgtcatcacg aagtattaac
    16261 tgacgttaga tggacctctt taatgataga acaattaata aataatgcac ttaagtatgc
    16321 gagaggtaaa gatatatgga ttgaatttga tgagcaatcc aatcaattac acgtaaaaga
    16381 taatggtatc ggtattagtg aagcggactt gcctaaaata tttgataagg gctattcagg
    16441 ttataatggc cagcgccaaa gtaactcaag tgggattggt ttatttatcg taaaacaaat
    16501 ttcaacacac acaaaccatc ctgtttcagt cgtatctaaa caaaatgagg gtacaacatt
    16561 tacgattcaa tttccagatg aataaaaact ttcaatattg taagtatact agta
//
LOCUS       DAXWFK010000046        16550 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.046, whole genome shotgun sequence.
ACCESSION   DAXWFK010000046 DAXWFK010000000
VERSION     DAXWFK010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16550
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.046"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            218..1300
                     /locus_tag="ACGEXS_001163"
     CDS             218..1300
                     /locus_tag="ACGEXS_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFF8012182.1"
                     /translation="MDNVTRYKITESSQSSSQAYYHLSFELSEQQSYASEHIVRAIRK
                     RQTILLYAVTGAGKTEMMFQGIQYARIQGDNIAIVSPRVDVVVEISKRIKDAFLNEDI
                     DILHQQSRQQFEGHFVVCTVHQLYRFKQHFDTIFIDEVDAFPLSMDKNLQQALKSSSK
                     VEHATIYMTATPPKQLLSEIPHENIIKLPARFHKKSLPVPKYRYFKLNNKKIQKMLYR
                     ILQDQINNQRYTLVFFNNIETMIKTFSVYKQKITKLTYVHSEDVFRFEKVEQLRNGHF
                     DVIFTTTILERGFTMANLDVVVIDAHQYTQEALIQIAGRVGRKLECPTGKVLFFHEGV
                     SMNMIQAKKEIQRMNKLALKRGWIDE"
     gene            1293..1967
                     /locus_tag="ACGEXS_001164"
     CDS             1293..1967
                     /locus_tag="ACGEXS_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFF8012183.1"
                     /translation="MNNCLSCGAKLYENITIYNLFKKPNRLCDRCKENWDNIKLDIKA
                     RRCSRCLKHLNQDEAYCLDCKFLSAHFNLMEQLYCQFQYDGLMKEMIHQYKFLKDYYL
                     CELLAHLIEIPQTSYDYIVPIPSSPAHDLSRTFNPVEAVLKAKGIRFDKILKMSNRPK
                     QSHLTKKERLADENPFIIDTELDLNGKEILLVDDIYTTGLTIHRAGCKLYAKNIRKFK
                     VFAFAR"
     gene            2028..2600
                     /gene="raiA"
                     /locus_tag="ACGEXS_001165"
     CDS             2028..2600
                     /gene="raiA"
                     /locus_tag="ACGEXS_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFF8012184.1"
                     /translation="MIRFEIHGDNLTITDAIRNYIEEKIGKLERYFNDVPNAVAHVKV
                     KTYSNSATKIEVTIPLKNVTLRAEERNDDLYAGIDLINNKLERQVRKYKTRINRKSRD
                     RGDQEVFVAELQEMQETQVDNDAYDDNEIEIIRSKEFSLKPMDSEEAVLQMNLLGHDF
                     FVFTDRETDGTSIVYRRKDGKYGLIQTSEQ"
     gene            3014..5545
                     /gene="secA"
                     /locus_tag="ACGEXS_001166"
     CDS             3014..5545
                     /gene="secA"
                     /locus_tag="ACGEXS_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFF8012185.1"
                     /translation="MGFLSKILDGNNKEIKQLGKLADKVIALEEKTAILTDEEIRNKT
                     KQFQTELADIDNVKKQNDYLDKILPEAYALVREGSKRVFNMTPYKVQIMGGIAIHKGD
                     IAEMRTGEGKTLTATMPTYLNALAGRGVHVITVNEYLSSVQSEEMAELYNFLGLTVGL
                     NLNSKTTEEKREAYAQDITYSTNNELGFDYLRDNMVNYSEDRVMRPLHFAIIDEVDSI
                     LIDEARTPLIISGEAEKSTSLYTQANVFAKMLKQDEDYKYDEKTKAVHLTEQGADKAE
                     RMFKVENLYDVQNVDVISHINTALRAHVTLQRDVDYMVVDGEVLIVDQFTGRTMPGRR
                     FSEGLHQAIEAKEGVQIQNESKTMASITFQNYFRMYNKLAGMTGTAKTEEEEFRNIYN
                     MTVTQIPTNKPVQRNDKSDLIYISQKGKFDAVVEDVVEKHKAGQPVLLGTVAVETSEY
                     ISNLLKKRGIRHDVLNAKNHEREAEIVAGAGQKGAVTIATNMAGRGTDIKLGEGVEEL
                     GGLAVIGTERHESRRIDDQLRGRSGRQGDKGDSRFYLSLQDELMIRFGSERLQKMMSR
                     LGLDDSTPIESKMVSRAVESAQKRVEGNNFDARKRILEYDEVLRKQREIIYNERNSII
                     DEEDSSQVVDAMLRSTLQRSINYYINTADDEPEYQPFIDYINDIFLQEGDITEDDIKG
                     KDAEDIFEVVWAKIEAAYQSQKDILEEQMNEFERMILLRSIDSHWTDHIDTMDQLRQG
                     IHLRSYAQQNPLRDYQNEGHELFDIMMQNIEEDTCKFILKSVVQVEDNIEREKTTEFG
                     EAKHVSAEDGKEKVKPKPIVKGDQVGRNDDCPCGSGKKFKNCHGK"
     gene            5587..5691
                     /locus_tag="ACGEXS_001167"
     CDS             5587..5691
                     /locus_tag="ACGEXS_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012186.1"
                     /translation="MLWEESFYFTSVKYFKKCEEVVKRCYVLSFKKSV"
     gene            5860..6970
                     /gene="prfB"
                     /locus_tag="ACGEXS_001168"
     CDS             join(5860..5931,5933..6970)
                     /gene="prfB"
                     /locus_tag="ACGEXS_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667688.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFF8012187.1"
                     /translation="MELSEIKRNIDKYNQDLTQIRGSLDLENKETNIQEYEEMMAEPN
                     FWDNQTKAQDIIDKNNALKAIVNGYKTLQAEVDDMDATWDLLQEEFDEEMKEDLEQEV
                     INFKAKVDEYELQLLLDGPHDANNAILELHPGAGGTESQDWANMLFRMYQRYCEKKGF
                     KVETVDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHRLVRISPFDSSGRRHTSFAS
                     CDVIPDFNNDEIEIEINPDDITVDTFRASGAGGQHINKTESAIRITHHPSGIVVNNQN
                     ERSQIKNREAAMKMLKSKLYQLKLEEQAREMAEIRGEQKEIGWGSQIRSYVFHPYSMV
                     KDHRTNEETGKVDAVMDGDIGPFIESYLRQTMSHD"
     gene            7124..7231
                     /locus_tag="ACGEXS_001169"
     CDS             7124..7231
                     /locus_tag="ACGEXS_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012188.1"
                     /translation="MCLLKQHMQCAFSNITYGDFQGIELIIENVLNYMV"
     gene            7379..8218
                     /locus_tag="ACGEXS_001170"
     CDS             7379..8218
                     /locus_tag="ACGEXS_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HFF8012189.1"
                     /translation="MKKSLTVTVSSVLAFLALNNAAHAQQHGTQVKTPVQHNYVSNVQ
                     AQTQSPTTYTVVAGDSLYKIALEHHLTLNQLYSYNPGVTPLIFPGDVISLVPQNKVKQ
                     TKAVKSPVRKASQAKKVVKQPVQQASKKVVVKQAPKQAVTKTVNVAYKPAQVQKSVPT
                     VPVAHNYNKSVANRGNLYAYGNCTYYAFDRRAQLGRSIGSLWGNANNWNYAAKVAGFK
                     VDKTPEVGAIFQTAAGPYGHVGVVESVNPNGTITVSEMNYAGFNVKSSRTILNPGKYN
                     YIH"
     gene            8392..9042
                     /locus_tag="ACGEXS_001171"
     CDS             8392..9042
                     /locus_tag="ACGEXS_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFF8012190.1"
                     /translation="MGVHQYFKRLSDMERLIRLPGKFKYFEHNVAAHSFKVTKIAQYL
                     ATVEEYHGRKINWKSLYEKALNHDFAEVFTGDIKTPVKYASSELKKLFSQVEEEMVET
                     FIEEEIPLQYRDVYKQRLQEGKDDSLEGQILSVADKIDLLYETFGEIQKRNPEELFFE
                     IYEMSLETIIQFDHLASVQDFINNIIPEMLTENFIPRTELRETTMNILNKRKEENE"
     gene            9039..9275
                     /locus_tag="ACGEXS_001172"
     CDS             9039..9275
                     /locus_tag="ACGEXS_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbA family protein"
                     /protein_id="HFF8012191.1"
                     /translation="MIWYFSAAFFPCVLVVLFSVITRSKWVGTILTLILIGASIYKEY
                     FHNEWIIFIDVVSLLAGYLIIDQLEFHKHQDEDR"
     gene            9544..11529
                     /gene="uvrB"
                     /locus_tag="ACGEXS_001173"
     CDS             9544..11529
                     /gene="uvrB"
                     /locus_tag="ACGEXS_001173"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002473941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFF8012192.1"
                     /translation="MVEHYPFKIHSDFEPQGDQPQAIKEIVEGIKAGKRHQTLLGATG
                     TGKTFTMSNVIKEVGKPTLIIAHNKTLAGQLYSEFKEFFPENRVEYFVSYYDYYQPEA
                     YVPSTDTFIEKDASINDEIDQLRHSATSALFERDDVIIIASVSCIYGLGNPEEYKDLV
                     VSVRVGMEMDRSELLRKLVDVQYTRNDIDFQRGTFRVRGDVVEIFPASKEELCIRVEF
                     FGDEIDRIREVNYLTGEVLKEREHFAIFPASHFVTREEKLKVAIERIEKELEERLKEL
                     RDENKLLEAQRLEQRTNYDLEMMREMGFCSGIENYSVHLTLRPLGSTPYTLLDYFGDD
                     WLVMIDESHVTLPQVRGMYNGDRARKQVLVDHGFRLPSALDNRPLKFEEFEEKTKQLV
                     YVSATPGPYEIEHTDKMVEQIIRPTGLLDPKIEVRPTENQIDDLLSEIQTRVERNERV
                     LVTTLTKKMSEDLTTYMKEAGIKVNYLHSEIKTLERIEIIRDLRMGTYDVIVGINLLR
                     EGIDIPEVSLVVILDADKEGFLRSNRSLIQTIGRAARNDKGEVIMYADKMTDSMKYAI
                     DETQRRREIQMKHNEKHGITPKTINKKIHDLISATVENDENNDKAQTVIPKKMTKKER
                     QKTIDNIEKEMKQAAKDLDFEKATELRDMLFELKAEG"
     gene            11537..14383
                     /gene="uvrA"
                     /locus_tag="ACGEXS_001174"
     CDS             11537..14383
                     /gene="uvrA"
                     /locus_tag="ACGEXS_001174"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFF8012193.1"
                     /translation="MKEPSIVVKGARAHNLKDIDIELPKNKLIVMTGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGQMDKPDVDTIEGLSPAISIDQKTTSKNPRSTVAT
                     VTEIYDYIRLLYARVGKPYCPNHNIEIESQTVQQMVDRIMELEARTKIQLLAPVIAHR
                     KGSHEKLIEDIGKKGYVRLRIDGEIVDVNDVPTLDKNKNHTIEVVVDRLVVKDGIETR
                     LADSIETALELSEGQLTVDVIDGEDLKFSESHACPICGFSIGELEPRMFSFNSPFGAC
                     PTCDGLGQKLTVDVDLVVPDKDKTLNEGAIEPWIPTSSDFYPTLLKRVCEVYKINMDK
                     PFKKLTERQRDILLYGSGDKEIEFTFTQRQGGTRKRTMVFEGVVPNISRRFHESPSEY
                     TREMMSKYMTELPCETCHGKRLSREALSVYVGGLNIGEVVEYSISQALNYYKNIDLSE
                     QDQAIANQILKEIISRLTFLNNVGLEYLTLNRASGTLSGGEAQRIRLATQIGSRLTGV
                     LYVLDEPSIGLHQRDNDRLINTLKEMRDLGNTLIVVEHDDDTMRAADYLVDIGPGAGE
                     HGGQIVSSGTPQKVMKDKKSLTGQYLSGKKRIEVPEYRRPASDRKISIRGARSNNLKG
                     VDVDIPLSIMTVVTGVSGSGKSSLVNEVLYKSLAQKINKSKVKPGLYDKIEGIDQLDK
                     IIDIDQSPIGRTPRSNPATYTGVFDDIRDVFAQTNEAKIRGYQKGRFSFNVKGGRCEA
                     CKGDGIIKIEMHFLPDVYVPCEVCDGKRYNRETLEVTYKGKNIADILEMTVEEATQFF
                     ENIPKIKRKLQTLVDVGLGYVTLGQQATTLSGGEAQRVKLASELHKRSTGKSIYILDE
                     PTTGLHVDDISRLLKVLNRLVENGDTVVIIEHNLDVIKTADYIIDLGPEGGSGGGTIV
                     ATGTPEDIAQTKSSYTGKYLKEVLERDKQNTEDK"
     gene            14990..15922
                     /gene="hprK"
                     /locus_tag="ACGEXS_001175"
     CDS             14990..15922
                     /gene="hprK"
                     /locus_tag="ACGEXS_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFF8012194.1"
                     /translation="MLTTEKLVETLKLDLIAGEEGLSKPIKNADISRPGLEMAGYFSH
                     YASDRIQLLGTTELSFYNLLPDKDRAGRMRKLCRPETPAIIVTRGLQPPEELVEAAKE
                     LNTPLIVAKDATTSLMSRLTTFLEHALAKTTSLHGVLVDVYGVGVLITGDSGIGKSET
                     ALELVKRGHRLVADDNVEIRQINKDELIGKPPKLIEHLLEIRGLGIINVMTLFGAGSI
                     LTEKRIRLNINLENWNKQKLYDRVGLNEETLSILDTEITKKTIPVRPGRNVAVIIEVA
                     AMNYRLNIMGINTAEEFSERLNEEIIKNSHKSEE"
     gene            15928..>16550
                     /gene="lgt"
                     /locus_tag="ACGEXS_001176"
     CDS             15928..>16550
                     /gene="lgt"
                     /locus_tag="ACGEXS_001176"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFF8012195.1"
                     /translation="MGIVFNYIDPVAFNLGPLSVRWYGIIIAVGILLGYFVAQRALVK
                     AGLHKDTLVDIIFYSALFGFIAARIYFVIFQWPYYAENPSEIIKIWHGGIAIHGGLIG
                     GFIAGVIVCKVKNLNPFQIGDIVAPSIILAQGIGRWGNFMNHEAHGGSVSRAFLEQLH
                     LPNFIIENMYINGQYYHPTFLYESIWDVAGFIILVNIRKHLKLGETF"
BASE COUNT         6092 a         2289 c         3215 g         4954 t
ORIGIN      
        1 ggttttaagc attaaacatt acgggaaatt aattcatcct catacttcac ttactaatga
       61 aaaaattaaa aaagaagtaa caggtgtcat caaacaaaat tcaaactatt attgtgttca
      121 atgtgaaagt acaaatccaa agcattttta tcagtatgat tcctcagtac attccaagaa
      181 aattgtatat tgcagaaatt gtatatcact gggtcgaatg gataatgtaa caagatataa
      241 aataacagag agttcgcaaa gttcatcaca agcatattat catctctcat ttgaattgtc
      301 ggaacagcag tcttatgcct cagaacatat tgttcgagcc attagaaaga gacaaacgat
      361 tttgttatat gccgtaacag gtgcaggtaa gacagaaatg atgtttcaag gcattcaata
      421 tgcaagaata cagggagata atatagctat tgtgtcacca cgtgtagatg ttgttgtaga
      481 aattagtaaa cgtattaaag acgcatttct taatgaagat atagacatac tacaccagca
      541 atcaagacaa caatttgaag ggcattttgt tgtatgcaca gtgcatcaac tttaccgatt
      601 caaacagcac tttgatacta tttttattga tgaagtcgat gcctttcctt tatcaatgga
      661 taaaaattta caacaagcat tgaagtcatc ttctaaagtt gaacatgcaa caatttatat
      721 gacagcaaca ccaccgaaac aacttctgtc agagattccc cacgaaaata taattaaatt
      781 gccagctcgc tttcataaaa aatcacttcc agttcctaaa tatcgttatt tcaaacttaa
      841 taataagaag attcagaaaa tgttataccg aattttacaa gatcaaatta ataatcaacg
      901 ttatacactg gtgtttttta acaatataga aacaatgatt aaaacatttt cggtttataa
      961 gcagaaaatt actaaattaa catacgtcca tagcgaggat gtttttcgct ttgaaaaagt
     1021 tgaacaatta aggaatggac atttcgatgt catttttact acgacaatat tagaacgtgg
     1081 atttacaatg gcaaatttgg atgttgttgt tatcgatgca catcaatata ctcaagaggc
     1141 tttaatacaa attgctggac gtgttggacg aaaattagaa tgtcctactg gaaaagtatt
     1201 gttttttcat gaaggggtaa gtatgaatat gattcaagct aaaaaagaga ttcaaaggat
     1261 gaacaaatta gcattaaaaa gaggttggat tgatgaataa ttgtttgagt tgtggtgcta
     1321 agttatatga aaatataacc atttataatt tgttcaagaa acctaataga ttatgtgaca
     1381 gatgcaaaga gaattgggac aatattaaac ttgatattaa agcaaggcga tgttcaaggt
     1441 gcttaaaaca cttaaatcaa gatgaagcgt attgtttaga ctgcaagttt ctatcggcac
     1501 actttaattt aatggaacaa ttatattgtc aatttcaata tgacggttta atgaaagaga
     1561 tgatacatca gtataaattt ttgaaagact attatttatg tgaattattg gcacatttga
     1621 ttgaaatacc acaaacatct tatgactata ttgtgccaat tccttcttcg ccggcacatg
     1681 atttatctag aacatttaac ccggtagaag cagtactaaa agctaaaggg attcgctttg
     1741 ataagatttt aaagatgtca aatagaccaa aacagtctca tttaactaag aaagagcgtc
     1801 tggcagatga aaatccattt attattgata cggaattaga tttaaatggt aaggaaatat
     1861 tactcgttga cgatatttat acaactggat taacaattca tcgtgcaggg tgtaaattat
     1921 atgctaaaaa tatcagaaaa ttcaaagtgt ttgcgtttgc acgatagcgt aaaaatgtta
     1981 aaatataata aagagttacc aataaagagg tttaaggaga gattactatg attagatttg
     2041 aaattcatgg agataacctc actatcacag atgctattcg caactatatt gaggaaaaaa
     2101 ttggtaagtt ggaacgttat tttaatgacg taccaaatgc agtggcgcat gttaaagtta
     2161 aaacttattc aaattcagct actaaaattg aagtaacaat tccattgaaa aatgttacgt
     2221 taagagctga agagcgaaac gatgatttat acgcaggtat tgatttaatt aataataaac
     2281 ttgaaagaca agttcgaaaa tataaaacac gtattaatcg taagagccgt gatcgaggag
     2341 atcaagaagt gtttgttgcc gaattacaag aaatgcaaga aacacaagtt gataatgacg
     2401 cttacgatga taacgagata gaaattattc gttcaaaaga attcagctta aaaccaatgg
     2461 attcagaaga agcggtatta caaatgaatc tattaggtca tgacttcttt gtattcacag
     2521 acagagaaac tgatggaaca agtatcgttt accgccgtaa agacggtaaa tatggcttga
     2581 ttcaaactag tgaacaataa attaagttta aagcacttgt gtttttgcac aagtgctttt
     2641 ttatactcca aaagcaaatt atgactattt catagttcga taatgtaatt tgttgaatga
     2701 aacatagtga ctatgctaat gttaatggat gtatatattt gaatgttaag ttaataatag
     2761 tatgtcagtc tattgtatag tccgagtcga aaatcgtaaa atatttataa tataatttat
     2821 taggaagtat aattgcgtat tgagaatata tttattagtg ataaacttgt tgacaacaga
     2881 atgtgaatga agtatgtcat aaatatattt atattgattc tacaaatgag taaataagta
     2941 taattttcta actataaatg ataagatata ttgttgtagg ccaaacagtt ttttagctaa
     3001 aggagcgaac gaaatgggat ttttatcaaa aattcttgat ggcaataata aagaaattaa
     3061 acagttaggt aaacttgctg ataaagtaat cgctttagaa gaaaaaacgg caattttaac
     3121 tgatgaagaa attcgtaata aaacgaaaca attccaaaca gaattagctg acattgataa
     3181 tgtcaaaaag caaaatgatt atttagataa aattttacca gaagcatatg cacttgttag
     3241 agaaggctct aaacgtgtat tcaatatgac accatataaa gttcaaatta tgggtggtat
     3301 tgcaattcat aaaggtgata tcgctgagat gagaacaggt gaaggtaaaa cattaacagc
     3361 gacaatgcca acatacttaa atgcattagc tggtagaggt gttcacgtta ttacagtcaa
     3421 tgaatactta tcaagtgttc aaagtgaaga aatggctgag ttatataact tcttaggttt
     3481 gactgtcgga ttaaacttaa acagtaagac gacagaagaa aaacgtgaag catacgcaca
     3541 agacattact tacagtacta ataatgagct aggttttgat tacttacgag ataacatggt
     3601 gaattattct gaagatagag taatgcgtcc attacatttt gcaatcattg atgaggttga
     3661 ctcaatttta atcgacgagg cacgtacgcc attaattatt tctggtgaag ctgaaaagtc
     3721 aacgtcactt tatacacaag caaatgtttt tgcgaaaatg ttaaaacagg acgaagatta
     3781 taaatacgat gaaaaaacga aagctgtaca tttaacagaa caaggtgcgg ataaagctga
     3841 acgtatgttc aaagttgaaa acttatatga tgtacaaaat gttgatgtta ttagtcatat
     3901 caacacagct ttacgtgcgc acgttacatt acaacgtgac gtagactata tggttgttga
     3961 tggcgaagta ttaattgtcg atcaatttac aggacgtaca atgccaggcc gtcgtttctc
     4021 ggaaggttta caccaagcta ttgaagcgaa ggaaggcgtt caaattcaaa atgaatctaa
     4081 aactatggcg tctattacat tccaaaacta tttcagaatg tacaataaac ttgcgggtat
     4141 gacaggtaca gctaaaactg aagaagaaga atttagaaat atttataaca tgacagtaac
     4201 tcaaattccg acaaataaac ctgtgcaacg taacgataag tctgatttaa tttacattag
     4261 ccaaaaaggt aaatttgatg cagtagtaga agatgttgtt gaaaaacaca aggcagggca
     4321 accagtgcta ttaggtactg ttgcagttga gacttctgaa tatatttcaa atttacttaa
     4381 aaaacgtggt atccgtcatg atgtgttaaa tgcgaaaaat catgaacgtg aagctgaaat
     4441 tgttgcaggc gctggacaaa aaggtgccgt tactattgcc actaacatgg ctggtcgtgg
     4501 tacagatatc aaattaggtg aaggcgtaga ggaattaggc ggtttagcag taataggtac
     4561 agagcgacat gaatctcgtc gtattgatga ccagttacgt ggtcgttctg gacgtcaagg
     4621 tgataaaggg gatagtcgct tctatttatc attacaagat gaattaatga ttcgttttgg
     4681 ttctgaacgt ttacagaaaa tgatgagccg actaggttta gatgactcta caccaattga
     4741 atcaaaaatg gtatcaagag ctgtagaatc agcacaaaaa cgtgtagaag gtaataactt
     4801 cgacgcgcgt aaacgtatct tagaatacga tgaagtatta cgtaaacaac gtgaaattat
     4861 ctataacgaa agaaatagta ttattgatga agaagacagc tctcaagttg tagatgcaat
     4921 gctacgttca acgttacaac gtagtatcaa ttactatatt aatacagcag atgacgagcc
     4981 tgaatatcaa ccattcatcg actacattaa tgacatcttc ttacaagaag gtgacattac
     5041 agaggatgat atcaaaggta aagatgctga agatattttc gaagtcgttt gggctaagat
     5101 tgaagcagca tatcaaagtc aaaaagatat cttagaagaa caaatgaatg agtttgagcg
     5161 tatgatttta cttcgttcta ttgatagcca ttggactgat catatcgaca caatggatca
     5221 attacgtcaa ggtattcact tacgttctta tgcacaacaa aatccattac gtgactatca
     5281 aaatgaaggt catgaattat ttgatatcat gatgcaaaat attgaagaag atacttgtaa
     5341 attcatttta aaatctgtag tacaagttga agataatatt gaacgtgaaa aaacaacaga
     5401 gtttggtgaa gcgaagcacg tttcagctga agatggtaaa gaaaaagtga aaccgaaacc
     5461 aatcgttaaa ggcgatcaag ttggtcgtaa cgatgattgt ccatgtggta gtggtaaaaa
     5521 attcaaaaat tgccatggaa aataaatgat ataaaataac tccttccaat taaacaccta
     5581 tagtttgtgt tatgggagga gtctttttat tttacaagcg ttaaatactt taaaaaatgt
     5641 gaagaagttg ttaaacgttg ttatgtactt agttttaaaa aatcggttta ggcatatgtc
     5701 gatgataaat gtactgattt ttaacaataa atgcataaac taattgtcag tgtgcttata
     5761 tttcttaaca ttgttattta acaaaattat gttaaaattt agcattataa aagatgcaaa
     5821 tcaatgactt gaattgaaat ataaatagga gcgaatgcta tggaattatc agaaatcaaa
     5881 cgaaatatag ataagtataa tcaagattta acacaaatta gggggtctct ttgacttaga
     5941 gaacaaagaa actaatattc aagaatatga agaaatgatg gcagaaccta atttttggga
     6001 taaccaaacg aaagcgcaag atattataga taaaaataat gcgttaaaag caatagttaa
     6061 tggttataaa acactacaag cagaagtaga tgacatggat gctacttggg atttattaca
     6121 agaagaattt gatgaagaaa tgaaagaaga cttagagcaa gaggtcatta attttaaggc
     6181 taaagtggat gaatacgaat tgcaattatt attagatggg cctcacgatg ccaataacgc
     6241 aattctagag ttacatcctg gtgcaggtgg cacggagtct caagattggg ctaatatgct
     6301 atttagaatg tatcaacgtt attgtgagaa gaaaggcttt aaagttgaaa ctgttgatta
     6361 tctacctggg gatgaagcgg ggattaaaag tgtaacattg ctcatcaaag ggcataatgc
     6421 ttatggttat ttaaaagctg aaaaaggtgt acaccgacta gtacgaattt ctccatttga
     6481 ttcatcagga cgtcgtcata catcatttgc atcatgcgac gttattccag attttaataa
     6541 tgatgaaata gagattgaaa tcaatccgga tgatattaca gttgatacat tcagagcttc
     6601 tggtgcaggt ggtcagcata ttaacaaaac tgaatcggca atacgaatta cccaccaccc
     6661 ctcaggtata gttgttaata accaaaatga acgttctcaa attaaaaacc gtgaagcagc
     6721 tatgaaaatg ttaaagtcta aattatatca attaaaattg gaagagcagg cacgtgaaat
     6781 ggctgaaatt cgtggcgaac aaaaagaaat cggctgggga agccaaatta gatcatatgt
     6841 tttccatcca tactcaatgg tgaaagatca tcgtacgaac gaagaaacag gtaaggttga
     6901 tgcagtgatg gatggagaca ttggaccatt tatcgaatca tatttaagac agacaatgtc
     6961 gcacgattaa tatatatttt aaaaccgagg ctctaaaagg gcgtcggttt ttggtttttt
     7021 taaaggtagc taaataaatt gtaaattaga ttttggaata tgatttgttt atgaatattt
     7081 aagtacaatt cggtagatag agttagaata tattttttaa aagttgtgtt tgttaaaaca
     7141 acatatgcag tgtgcattta gtaatattac ctatggcgat tttcaaggta ttgaattaat
     7201 tattgaaaac gttctcaatt acatggtatg aatacatttt acactatgat aaaaggttgt
     7261 attcttttta tattgttaac catttgatta catcgttata acaatagctt ttgacaaaat
     7321 gtattgtgct atagtatttg catacttaaa atactaacag caaaggaatg acagcaagat
     7381 gaaaaaatct cttacagtga cggtttcgtc agtgttagct tttttagctt taaataatgc
     7441 agcacatgca caacaacatg gcacacaagt aaaaacacct gttcaacata attatgtatc
     7501 aaatgttcaa gcacaaacgc aatcaccgac aacttataca gtagttgctg gcgattcatt
     7561 atataagatt gctttagagc atcacttaac gttgaatcaa ttatattcat acaatcctgg
     7621 tgtaacacct ttaatttttc ctggtgacgt gatttcactt gtgcctcaaa ataaagtgaa
     7681 acaaactaaa gcggttaaat caccagtaag aaaagcaagc caagctaaaa aggtagtaaa
     7741 acaacctgta caacaagcat ctaaaaaagt agtagttaag caagcaccta agcaagcagt
     7801 aactaagaca gttaatgtag catacaaacc tgctcaagta caaaaatcag taccaactgt
     7861 acctgttgca cataactaca ataaatcagt tgctaacaga ggaaacttat atgcttatgg
     7921 aaactgcaca tattatgctt tcgatcgtcg tgcacaatta ggtagaagta taggaagttt
     7981 atggggcaat gcaaataact ggaattacgc agcaaaagtt gcaggattta aagtagataa
     8041 aacaccagaa gttggcgcta ttttccaaac agctgctggt ccatatggac atgttggtgt
     8101 tgttgaatct gtaaacccta atggaacaat tactgtttct gaaatgaact atgctggatt
     8161 taatgttaaa tcttcaagaa caattttaaa tccaggaaaa tataattaca tccactaagt
     8221 aatatatcaa gacaagacta tcctcttagc ctgtttaagt aacaggttga gaggattttt
     8281 tggtatcatt taatcagagt tatataaaga agatatttaa atgatattta attgattgat
     8341 atgtaaaaag aaagtataat ttatgattaa ttaatggagg aggtaattga aatgggtgta
     8401 catcaatatt ttaaaagatt atcagatatg gaaagactta taagattacc tggaaaattt
     8461 aaatattttg aacacaatgt tgcagcacac tcctttaaag taactaaaat tgcacaatat
     8521 ctagcaacag ttgaagaata tcatggacga aagattaatt ggaaaagctt atatgaaaaa
     8581 gcattaaatc atgatttcgc cgaagtgttt actggtgata taaaaacacc tgttaaatat
     8641 gcgagtagtg aattaaaaaa attattttcg caagttgaag aagaaatggt agagaccttt
     8701 attgaagaag aaattccatt acaatataga gatgtttata agcaacgact gcaagaaggt
     8761 aaagatgatt cattagaagg ccaaatactt tcagttgctg ataaaattga tttgctttat
     8821 gaaacatttg gagaaataca aaaacgtaat cccgaagaat tatttttcga aatttatgaa
     8881 atgagtctag aaacaattat tcaatttgac catttagcat ctgtacaaga ttttattaat
     8941 aatatcattc cagaaatgtt gactgaaaac tttataccta gaacagaatt aagagaaaca
     9001 accatgaaca ttttaaataa aagaaaagag gaaaatgaat gatatggtat tttagcgcag
     9061 cattctttcc atgtgtcttg gtagtattat ttagtgtaat aacaagaagt aaatgggtcg
     9121 gtactattct gacattaatt ttaattggtg cctcaatcta taaagagtat ttccataacg
     9181 agtggattat ttttattgat gtagtgtcat tattagctgg ttatttaatt atagatcaac
     9241 tcgaatttca taaacatcaa gatgaagatc gctaagatta actttaaaat aatgtttcaa
     9301 acaaatttgt tgaaacaaaa tgatgattaa tataatgtgt atttacatac taaaaataac
     9361 aagataaacg atttgattta aggcaagcat agttagcaca actatgtttg tttttctttg
     9421 ttcgacattt ttacgaacaa acgtttgctt tttgtgtgac tactttgcta aaatatgtaa
     9481 tgagaaagca aagagttgag cggaaatata aataatacgt tgaaagagga ggcatatgtg
     9541 acaatggttg aacattatcc ttttaaaata cattctgatt ttgagcctca aggtgaccaa
     9601 ccgcaagcaa ttaaagaaat cgtggaaggt attaaagcgg ggaaaagaca tcaaacttta
     9661 ttaggtgcta ctggcacagg gaaaacattt acgatgagta atgttattaa agaagttggg
     9721 aaaccaacgt taattatcgc gcataacaaa acattagcag gacaattata tagtgagttt
     9781 aaagaatttt ttcctgaaaa cagggtggaa tactttgtaa gttactatga ttattatcaa
     9841 ccagaggcat acgtaccgtc tactgacact tttattgaaa aagatgcctc aatcaatgat
     9901 gaaattgatc aactacgaca ttctgctaca agtgcattat ttgaacgcga tgatgtaatt
     9961 attattgcta gtgtaagttg tatatatggt ttaggtaatc ctgaagaata taaagattta
    10021 gtagtaagtg ttcgagttgg tatggaaatg gatagaagtg aattacttag aaaacttgta
    10081 gatgtgcaat atacacgaaa tgacatcgat ttccaacgag gaacgtttcg agtgcgtggt
    10141 gatgtagtgg aaatattccc agcctctaaa gaagaacttt gtataagggt tgagtttttc
    10201 ggcgatgaga ttgaccgtat ccgagaagtt aactacctaa caggtgaagt gttgaaagaa
    10261 agagaacatt ttgcgatatt cccagcttct cacttcgtaa cacgtgaaga aaagttgaaa
    10321 gttgcgattg aacgtattga aaaagaattg gaagaacgat tgaaagaatt acgagatgag
    10381 aataaattac tagaagcgca aaggttagaa cagcgtacca actatgattt agaaatgatg
    10441 cgagagatgg gattctgttc aggaattgaa aactattccg tacatttaac tttgcgacca
    10501 ctgggttcga caccatatac tttattggat tactttggcg atgattggtt agtaatgatt
    10561 gatgaatcac atgtgacatt accgcaagtt cgaggcatgt ataacggaga cagagcgcgt
    10621 aaacaagttt tggtggatca tgggtttaga ttaccgagtg cattagataa ccgtccactt
    10681 aaatttgaag aatttgaaga aaagacaaaa caacttgtgt atgtatctgc aacgcctgga
    10741 ccatacgaaa ttgaacatac ggataagatg gttgaacaaa ttattcgtcc tactggttta
    10801 ctggatccta agattgaggt tagacctact gaaaatcaaa ttgacgattt attaagtgaa
    10861 attcaaacaa gagttgagcg taatgaacgc gtacttgtta caacgctcac taaaaagatg
    10921 agtgaagatt taaccacata catgaaagaa gcgggtatta aagttaatta tctgcattca
    10981 gaaatcaaga cattagaacg aattgaaata attagagact tacgaatggg tacatatgat
    11041 gttatcgtag gtattaattt attaagagag ggtattgata taccagaagt ttctctagtt
    11101 gtcatattag atgcagataa agaagggttt ttacgttcta accgctcatt aattcaaaca
    11161 ataggtagag ctgcgcgtaa cgataaaggt gaagtcatta tgtatgccga taaaatgact
    11221 gattcgatga agtatgcaat tgatgagaca caacgtcgtc gagaaataca gatgaaacat
    11281 aatgaaaaac atggtattac acctaaaaca attaataaaa aaatacatga tttaattagt
    11341 gctactgttg aaaatgacga aaataatgac aaagcacaaa ctgtgatacc taagaagatg
    11401 acgaaaaaag aacgtcaaaa gacaatcgac aatatagaaa aagaaatgaa acaagcagcg
    11461 aaagatttag atttcgagaa agctacagaa ttaagagata tgttatttga attaaaagca
    11521 gaagggtgac aagtaaatga aagaaccatc catagtagta aaaggtgctc gtgcgcataa
    11581 cttgaaagat attgatatcg aactacctaa aaataaatta attgttatga caggtttatc
    11641 tgggtcaggt aaatcgtcat tagcattcga tactatatat gctgaaggac aacgacgtta
    11701 tgttgaatca ttaagtgcct atgcgcgtca atttttaggc caaatggaca aaccagatgt
    11761 tgatacaatt gaaggattat cgccagcaat ttcaatagat caaaaaacaa caagtaaaaa
    11821 tccaagatca actgtagcaa cagtaacaga aatatatgat tatatacgtt tgttatatgc
    11881 acgtgttggt aaaccttact gtccaaatca caatatagaa attgaatcgc aaacagtaca
    11941 acaaatggtt gaccgcatta tggaattaga ggcacgtaca aagattcaat tattagcacc
    12001 tgtcatcgct catcgtaaag gtagtcatga aaagctaatc gaagatattg gtaaaaaagg
    12061 ttatgtacgt ttaagaatcg atggcgaaat tgttgatgta aatgatgtac ctactttaga
    12121 taagaacaag aatcatacaa tagaagttgt tgtagaccga ttagttgtta aagatggaat
    12181 tgaaacacga ctagctgact ctatagaaac tgccttagag ctttcagaag gacaattaac
    12241 agtcgatgtc attgacgggg aagaccttaa gttttcagaa agccatgctt gtcctatatg
    12301 tggattttca atcggagagt tagaaccaag aatgtttagc tttaacagtc cttttggtgc
    12361 ttgtccgaca tgtgatggct taggccaaaa gttaacagtc gatgtagact tggttgttcc
    12421 cgacaaagat aagacgctaa acgaaggtgc aatagaacct tggataccga cgagttctga
    12481 tttttatcca acattgttaa aacgtgtttg tgaagtttat aaaatcaata tggataaacc
    12541 ttttaaaaag ttaacagaac gtcaacgtga tattttattg tatggttctg gtgacaaaga
    12601 aattgaattt acatttacac aacgtcaagg tggtactaga aaacgaacaa tggttttcga
    12661 gggtgtagtt cctaatataa gtagacgatt ccatgaatct ccttcagaat atacacgtga
    12721 aatgatgagt aaatatatga ctgaactacc ttgcgaaact tgtcatggaa agcgattgag
    12781 tcgtgaagcg ttatctgttt atgtaggtgg tttaaatatt ggtgaagtag tcgaatattc
    12841 aatcagtcaa gcgctgaact attataaaaa cattgatttg tcagaacaag atcaagcgat
    12901 tgcaaatcaa atattgaaag aaattatttc ccgactcact tttttaaata atgtgggact
    12961 tgaatattta acgttaaaca gagcttcagg tacactttca ggtggtgaag cacaacgtat
    13021 tcgattagca acgcaaattg ggtcgcgttt gactggtgtc ttatatgtat tagatgagcc
    13081 atcaattgga ctgcatcaaa gagataatga tcgattaatt aatacactta aagaaatgag
    13141 agatttagga aatactttaa ttgtagttga acacgatgat gatacaatgc gtgcggctga
    13201 ttacttagtg gatataggtc ctggtgctgg tgaacatgga gggcagattg tgtctagtgg
    13261 tactcctcaa aaggtaatga aagataaaaa atcattaaca ggacaatact tgagtggtaa
    13321 gaaacgtatt gaagtacctg aatatcgcag accggcttca gatcgtaaaa tttctatacg
    13381 tggagctaga agcaacaatc ttaaaggggt tgatgtggac ataccactat caatcatgac
    13441 ggttgttaca ggtgtatcag gttctggtaa aagctcatta gtaaatgaag tattatacaa
    13501 atcattagct caaaaaatta ataaatctaa agtaaagcca ggattgtacg ataagattga
    13561 aggtattgat caacttgata aaattattga tattgatcaa tcaccaatag gtagaacgcc
    13621 acgctctaat ccagcaacat atactggtgt gtttgatgat atacgtgatg tgtttgcgca
    13681 aacaaatgaa gctaaaattc gaggatatca aaaagggcgt tttagtttta atgtaaaagg
    13741 tggacgctgt gaagcttgta aaggtgacgg tattattaaa attgaaatgc attttttacc
    13801 tgatgtttat gttccttgtg aagtgtgtga tggtaaacga tataatcgtg agacactaga
    13861 ggttacttac aaaggtaaaa atattgctga cattttagaa atgactgttg aagaagcaac
    13921 acaatttttt gaaaatattc ctaagattaa gcgcaagtta caaacactag ttgatgttgg
    13981 tcttggatac gtcacattag gtcaacaagc tacaacgtta tcaggtggtg aggctcaacg
    14041 tgtgaaactt gcatctgaac ttcataaacg ttcaactggt aaatctattt atatcctaga
    14101 tgaaccgaca acagggttac atgttgacga tattagtaga ttattaaaag tattaaaccg
    14161 attagttgaa aatggtgata ctgttgtaat tattgaacat aacctagatg ttatcaaaac
    14221 agcagactat attatagact taggtcctga aggtggtagt ggcggtggta ctattgttgc
    14281 gactggcaca cccgaagata ttgctcagac aaagtcatca tatacaggaa agtatttaaa
    14341 agaagtactt gaacgagata aacaaaatac tgaagataaa taagattaaa agaagtgaag
    14401 gatgttataa atttatcctt cgcttctttt tattaattta gtaatgaata gtagaaagaa
    14461 aagatgcgta aaaagaatta tgttaagata gggtcaatct agagtagtta aacataaatc
    14521 gaactgggag tgggacagaa atgataaaga atcactaatg atttattatg tagtggttct
    14581 ttgtcattag ccacagctat tgtgtactta aaaataggaa tgcatgagtg caactcatgc
    14641 ataagaaata ctaatttcta aagaaaaagt atttctttat gttggggccc cgccaacttg
    14701 cattgtttgt agaatttctt ttcgaaattc tttatgttgg ggccccgcca actaattcca
    14761 atatatcatt gtagagctta ggtcattgat ttttggctcg gacttttatg gcgatatgaa
    14821 ccatgtaaat taagcaagca ataaattaat gattgatatt gacttgtaaa ataataacaa
    14881 taatgaacaa ttaatattta ttttagcttt tcaatgtaga ttggtgttat atttttgata
    14941 tgataagaag agatgtaaga gtagggataa atacaattga ggtgaaccca tgttaacgac
    15001 agaaaaacta gttgaaacat taaagttaga tttaatcgct ggtgaagaag gactatcgaa
    15061 gccaattaaa aatgctgata tatcaagacc gggcttagag atggcaggtt atttttcaca
    15121 ttatgcgtca gatagaatac aactattagg aacaacggaa ctatcgtttt acaatttatt
    15181 accagataag gatcgcgcag gtcgtatgcg taaactatgc agaccagaaa cgcctgcaat
    15241 tattgtgaca cgtggattgc agccaccaga agaattagtt gaagctgcaa aagaattaaa
    15301 taccccactt atagttgcta aagatgcgac tacaagttta atgagtcgct taacaacgtt
    15361 tttagagcat gcacttgcaa agacgacatc tttacatggt gttttagtag atgtttacgg
    15421 tgttggtgta ctaattaccg gtgattcagg aataggtaaa agtgagactg cgttggaatt
    15481 agttaaacgt gggcatagat tagtagcaga tgataatgta gaaatacgtc aaattaataa
    15541 agatgaacta atagggaaac caccaaagtt aatagaacat ctattagaaa tacgtggact
    15601 aggtattatc aatgttatga ctttatttgg cgcgggttca atattaactg aaaaacgaat
    15661 tagattaaat attaatttgg aaaactggaa caagcaaaag ttatatgacc gcgtaggtct
    15721 taatgaagag acgctaagta ttttagatac tgaaatcact aaaaaaacaa tacctgtaag
    15781 acctggtaga aatgttgcgg taattattga ggtcgctgca atgaactatc gattaaatat
    15841 catgggcatt aacacggccg aagaatttag tgaaagatta aatgaagaaa ttatcaagaa
    15901 cagtcataag agtgaggagt aggttgaatg ggtattgtat ttaactatat agatcctgtg
    15961 gcatttaact taggaccact gagtgtacga tggtatggaa ttatcattgc tgtcggaata
    16021 ttacttggtt actttgttgc acaacgtgca ctagttaaag caggattaca taaagatact
    16081 ttagtagata ttatttttta tagtgcacta tttggattta tcgcggcacg aatctatttt
    16141 gtgattttcc aatggccata ttacgcggaa aatccaagtg aaattattaa aatatggcat
    16201 ggtggaatag caatacatgg tggtttaata ggtggcttta ttgctggtgt tattgtatgt
    16261 aaagtgaaaa atttaaaccc atttcaaatt ggtgatatcg ttgcgccaag tataatttta
    16321 gcgcaaggaa ttggacgctg gggtaacttt atgaatcacg aggcacatgg tggatcggtg
    16381 tcacgcgctt ttttagaaca attacatttg cctaatttta taatagaaaa tatgtatatt
    16441 aacggccaat attatcatcc aacattctta tatgaatcca tttgggatgt cgctggattt
    16501 attatcttag ttaatattcg taaacattta aaattaggag aaacattctt
//
LOCUS       DAXWFK010000047        16398 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.047, whole genome shotgun sequence.
ACCESSION   DAXWFK010000047 DAXWFK010000000
VERSION     DAXWFK010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16398
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.047"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..6540
                     /gene="sasC"
                     /locus_tag="ACGEXS_001177"
     CDS             <1..6540
                     /gene="sasC"
                     /locus_tag="ACGEXS_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored aggregation protein SasC"
                     /protein_id="HFF8012196.1"
                     /translation="KYSIRKYKVGIFSTLIGTVLLLSNPNGAQALTTDNNVQSDTNQA
                     TPVNSQDKDVANNRGLANSAQNTPNQSATTNQATNQALVNHNNGSIVNQATPTSVQSS
                     TPSAQNNNHTDGNTTATETVSNANNNDVVSNNTALNVPTKTNENGSGGHLTLKEIQED
                     VRHSSNKPELVAIAEPASNRPKKRSRRAAPADPNATPADPAAAAVGNGGAPVAITAPY
                     TPTTDPNANNAGQNAPNEVLSFDDNGIRPSTNRSVPTVNVVNNLPGFTLINGGKVGVF
                     SHAMVRTSMFDSGDNKNYQAQGNVIALGRIHGTDTNDHGDFNGIEKALTVNPNSELIF
                     GFNTMTTKNGQGATNVIIKNADTNDTIAEKTVEGGPTLRLFKVPDNVRNLKIQFVPKN
                     DAITDARGIYQLKDGYKYYSFVDSIGLHSGSHVFVERRTMDPTATNNKEFTVTTSLKN
                     NGNSGASLDTNDFVYQVQLPEGVEYVNNSLTKDFPSNNSGVDVNDMNVTYDAANRVIT
                     IKSTGGGTANSPARLMPDKILDLRYKLRVNNVPTPRTVTFNETLTYKTYTQDFINSAA
                     ESHTVSTNPYTIDIIMNKDALQAEVDRRIQQADYTFASLDIFNGLKRRAQTILDENRN
                     NVPLNKRVSQAYIDSLTNQMQHTLIRSVDAENAVNKKVDQMEDLVNQNDELTDEEKQA
                     AIQVIEEHKNEIIGNIGDQTTDDGVTRIKDQGIQTLSGDTATPVVKPNAKKAIRDKAT
                     KQREIINATPDATEDEIQDALNQLATDETDAIDNVTNATTNADVETAKNNGINTIGAV
                     VPQVTHKKAARDAINQATATKRQQINSNREATQEEKNAALNELTQATNHALEQINQAT
                     TNADVDNAKGDGLNAINPIAPVTVVKQAARDAVSHDAQQHIAEINANPDATQEERQAA
                     IDKVNAAVTAANTNILNANTNADVEQVKTNAIQGIQAITPATKVKTDAKNAIDKSAET
                     QHNTIFNNNDATLEEQQAAQQLLDQAVATAKQNINAADTNQEVAQAKDQGTQNIVVIQ
                     PATQVKTDARNVVNDKAREAITNINATTGATREEKQEAINRVNTLKNRALTDIGVTST
                     TAMVNSIRDDAVNQIGAVQPHVTKKQTATGVLNDLATAKKQEINQNTNATTEEKQVAL
                     NQVDQELATAINNINQADTNAEVDQAQQLGTKAINAIQPNIVKKPAALAQINQHYNAK
                     LAEINATPDATNDEKNAAINTLNQDRQQAIESIKQANTNAEVDQAATVAENNIDAVQV
                     DVVKKQAARDKITAEVAKRIEAVKQTPNATDEEKQAAVNQINQLKDQAINQINQNQTN
                     DQVDTTTNQAVNAIDNVEAEVVIKPKAIADIEKAVKEKQQQIDNSLDSTDNEKEVASQ
                     ALAKEKEKALAAIDQAQTNSQVNQAATNGVSAIKIIQPETKVKPAAREKINQKANELR
                     AKINQDKEATAEERQVALDKINEFVNQAMTDITNNRTNQQVDDTTSQALDSIALVTPD
                     HIVRAAARDAVKQQYEAKKREIEQAEHATDEEKQVALNQLANNEKRALQNIDQAIANN
                     DVKRVETNGIATLKGVQPHIVIKPEAQQAIKASAENQVESIKDTPHATVDELDEANQL
                     ISDTLKQAQQEIENTNQDAAVTDVRNQTIKAIEQIKPKVRRKRAALDSIEENNKNQLD
                     AIRNTLDTTQDERDVAIDTLNKIVNTIKNDIAQNKTNAEVDRTETDGNDNIKVILPKV
                     QVKPAARQSVGVKAEAQNALIDQSDLSTEEERLAAKHLVEQALNQAIDQINHADKTAQ
                     VNQDSINAQNIISKIKPATTVKATALQQIQNIATNKINLIKANNEATDEEQNIAIAQV
                     EKELIKAKQQIASAVTNADLAYLLHDEKNEIREIEPVINRKASAREQLTTLFNDKKQA
                     IEANIQATVEERNSILAQLQNIYDTAIGQIDQDRSNAQVDKTASLNLQTIHDLDVHPI
                     KKPDAEKTINDDLARVTALVQNYRKVSNRNKADALKAITALKLQMDEELKTARTNADV
                     DAVLKRFNVALSDIEAVITEKENSLLRIDNIAQQTYAKFKAIATPEQLAKVKVLIDQY
                     VADGNRMIDEDATLNDIKQHTQFIVDEILAIKLPAEATKVSPKEIQPAPKVCTPIKKE
                     ETHESRKVEKELPNTGSEGMDLPLKEFALITGAALLARRRTKNEKES"
     gene            complement(6657..7925)
                     /locus_tag="ACGEXS_001178"
     CDS             complement(6657..7925)
                     /locus_tag="ACGEXS_001178"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFF8012197.1"
                     /translation="MYQTIIIGGGPSGLMAAVAASEQSSSVLLIEKKKGLGRKLKISG
                     GGRCNVTNRLPYAEIIKNIPGNGKFLYSPFSIFDNESIIDFFESRGVKLKEEDHGRMF
                     PVSNKAQDVVDTLVTTIERQHVTIKEEEAVSRIEVNTDQTFTVHTQNNSYESHSLVIA
                     TGGTSVPQTGSTGDGYKFAQDLGHTITELFPTEVPITSAEPFIKSNRLKGLSLKDVEL
                     SVLKKNGKKRISHQMDMLFTHFGISGPAALRCSQFVYKEQKNQKTQHISMAIDAFPEL
                     NHEQLKQHITSLLSDTPDKIIKNSLHGLIEERYLLFMLEQAGIDENTTSHHLSNQQLN
                     DLVNMFKGFVFKVNGTLPIDKAFVTGGGVSLKEIQPKTMMSKLVPGLFLCGEVLDIHG
                     YTGGYNITSALVTGHVAGLYAGHYSHASME"
     gene            8332..9993
                     /locus_tag="ACGEXS_001179"
     CDS             8332..9993
                     /locus_tag="ACGEXS_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFF8012198.1"
                     /translation="MSESKEMVRGTFLITISILITKVLGVLFIIPFNYLIGGQENMAP
                     FTYAYAPYNIAIAVATAGVPLAASKYVAKYNAIGAYKVSQKFYKSSFIVMSITGVLGF
                     LVLYFLAPYISELTLARNIHDKNGWSVDDITWIIRIISMVVIFIPVLATWRGIFQGYK
                     SMGPTAVSEVTEQIARVIFILIGSYLVLNVFDGSILLANGIATFAAAVGAIIGIFTLW
                     YYWRKRKHNIDRMVESDYTDIDVSYGKMYKEIIAYSIPFVIVSLNYPLFNLVDQFTHN
                     GALSLVGIPSQLQDIFFNMLNMSTNKIVMIPTSLSAGFAVSLIPYITKTFAEGRLHEM
                     HHQIRTSIGVLMFITVPASIGIMALAQPLFTVFYGYDPIVLGHDPNHDGSRLLFYYAP
                     VAILISLLSVTASMLQGIDKQKLTVYVILASVVIKLALNYPLIMLFHTPGAILSTSIA
                     LLFAIGCNFYILKKYAKFKFSYSWIHFAKIFLYSFIMMLGVELVFFLANLFLEPTKLG
                     YLIIIILGVTVGILIYGTITIKTRLADEFLGEIPEKLRRRVRFLR"
     gene            9990..10685
                     /locus_tag="ACGEXS_001180"
     CDS             9990..10685
                     /locus_tag="ACGEXS_001180"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFF8012199.1"
                     /translation="MRIDKFLANMGVGTRNEVKQLLKKGLVNVNEQVIKSPKTHIEPE
                     NDKITVRGELIEYIENVYIMLNKPKGYISATEDHHSKTVIDLIPEYQHLNIFPVGRLD
                     KDTEGLLLITNDGDFNHELMSPNKHVSKKYEVISANPITEDDIQAFKEGVTLTDGKVK
                     PAILTYIDNQTSHVTIYEGKYHQVKRMFHSIQNEVLHLRRIKIADLELDSNLDSGEYR
                     LLTENDFDKLNYK"
     gene            10702..11124
                     /locus_tag="ACGEXS_001181"
     CDS             10702..11124
                     /locus_tag="ACGEXS_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFF8012200.1"
                     /translation="MAKGNLFKAILGIGGAVAAVLVTRKDSRDKLKAEYNKYKQDPQS
                     YKDNAKDKATQLGTIANETIKEVKTNPKEYANRLKNNPKAFFKEEKSKFTEYDNKNDE
                     SIEKGKFDDEGGAAPNNNLRIVTEEDLKKNKNALSDKE"
     gene            11930..13339
                     /gene="sapep"
                     /locus_tag="ACGEXS_001182"
     CDS             11930..13339
                     /gene="sapep"
                     /locus_tag="ACGEXS_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mn(2+)-dependent dipeptidase Sapep"
                     /protein_id="HFF8012201.1"
                     /translation="MWKEKVQQYEDQIINDLKGLLAIESVRDDAKASEDAPVGPGPRK
                     ALDYMYEIAHRDGFTTHDVDHIAGRIEAGKGNDVLGILCHVDVVPAGDGWDSNPFEPV
                     VTEDAIIARGTLDDKGPTIAAYYAIKILEDMNVDWKKRIHMIIGTDEESDWKCTDRYF
                     KTEEMPTLGFAPDAEFPCIHGEKGITTFDLVQNKLTEDQDEPDYELITFKSGERYNMV
                     PDHAEARVLVKENMTDVIQDFEYFLEQNHLQGDSTVDSGILVLTVEGKAVHGMDPSIG
                     VNAGLYLLKFLASLNLDNNAQAFVAFSNRYLFNSDFGEKMGMKFHTDVMGDVTTNIGV
                     ITYDNENAGLFGINLRYPEGFEFEKAMDRFANEIQQYGFEVKLGKVQPPHYVDKNDPF
                     VQKLVTAYRNQTNDMTEPYTIGGGTYARNLDKGVAFGAMFSDSEDLMHQKNEYITKKQ
                     LFNATSIYLEAIYSLCVEE"
     gene            13343..14191
                     /gene="dat"
                     /locus_tag="ACGEXS_001183"
     CDS             13343..14191
                     /gene="dat"
                     /locus_tag="ACGEXS_001183"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047810 - D-alanine:2-oxoglutarate
                     aminotransferase activity [Evidence IEA];
                     GO_process: GO:0019480 - L-alanine oxidation to pyruvate
                     via D-alanine [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HFF8012202.1"
                     /translation="MEKIFLNGEFVSPSEAKVSYNDRGYVFGDGIYEYIRVYNGKLFT
                     VTEHYERFLRSANEIGLDLNYSVEELIELSRKLVDMNQIETGAIYIQATRGVAERNHS
                     FPTPEVEPAIVAYTKSYDRPYDHLENGVNGVTVEDIRWLRCDIKSLNLLGNVLAKEYA
                     VKYNAVEAIQHRGETVTEGSSSNAYAIKDGVIYTHPINNYILNGITRIVIKKIAEDYN
                     IPFKEETFTVDFLKNADEVIVSSTSAEVTPVIKLDGEPVNDGKVGPITRQLQEGFEKY
                     IESHSI"
     gene            14741..15532
                     /locus_tag="ACGEXS_001184"
     CDS             14741..15532
                     /locus_tag="ACGEXS_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFF8012203.1"
                     /translation="MEQFYQLGWTLDSAGGASGEAYMAEQDGQKLFLKRNSNPFIAAL
                     SAEGIVPKLVWTKRIETGEVVTAQHWKNGRELSSNEMKQTRVAHLLKKIHNSRPLLSM
                     LKRMEMEPITPEIMLNKINASLSREVLTHHIVRKSLTYLEEHIPSLDSRFFTVVHGDV
                     NHNNWLLSDRDELFLVDWEGAMIADPAIDIGMLLYNYVPQQQWSEWLETYGVQESLNL
                     NKRMKWYTVIQSIGLVQWYEEQKRYKDMNTWLKFLNEVMNSNMFI"
     gene            15547..16191
                     /gene="trmB"
                     /locus_tag="ACGEXS_001185"
     CDS             15547..16191
                     /gene="trmB"
                     /locus_tag="ACGEXS_001185"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFF8012204.1"
                     /translation="MRVRYKPWAEDYLKDHPELVDMDGQHAGKMTEWFDKTQPIHIEI
                     GSGMGQFITTLAAQNPHINYISMEREKSIVYKVLDKVKEMGLTNLKIICNDAIELNEY
                     FKDGEVSRIYLNFSDPWPKNRHAKRRLTYHTFLALYQQILNDEGDLHFKTDNRGLFAY
                     SLESMSQFGMYFTKINLNLHQEDDGSNILTEYEKKFSDKGSRIYRMEAKFHSQK"
BASE COUNT         6115 a         2512 c         2994 g         4777 t
ORIGIN      
        1 aaatatagta ttaggaagta taaagtaggc atattctcta ctttaatcgg aacagtttta
       61 ttactttcaa acccaaatgg tgcacaagcc ttaactacgg ataataatgt acaaagcgat
      121 actaatcaag caacacctgt aaattcacaa gataaagatg ttgctaataa tagaggttta
      181 gcaaatagtg cgcagaatac acctaatcaa tctgcaacaa ccaatcaagc aacgaatcaa
      241 gcattggtta atcataataa tggtagtata gtaaatcaag ctacgccaac atcagtgcaa
      301 tcaagtacgc cttcagcaca aaacaataat catacagatg gcaatacaac agcaactgag
      361 acagtgtcaa acgctaataa taatgatgta gtgtcgaata ataccgcatt aaatgtacca
      421 actaaaacaa atgaaaatgg ttcaggagga catctaactt taaaggaaat tcaagaagat
      481 gttcgtcatt cttcaaataa accagagcta gttgcaattg ctgaaccagc atctaataga
      541 ccgaaaaaga gaagtagacg tgcggcaccg gcagatccta atgcaactcc agcagatcca
      601 gcggctgcag cggtaggaaa cggtggtgca ccagttgcaa ttacagcgcc atatacgcca
      661 acaactgatc ctaatgccaa taatgcagga caaaatgcac ctaacgaagt gctgtcattt
      721 gatgacaatg gtattagacc aagtaccaac cgttctgtgc caacagtaaa cgttgttaat
      781 aacttgccgg gcttcacact aatcaatggt ggcaaagtag gggtgtttag tcatgcaatg
      841 gtaagaacga gcatgtttga ttcaggagat aataagaact atcaagcaca aggaaatgta
      901 attgcattag gtcgtataca tggaactgat acgaatgacc atggcgattt taatggtatc
      961 gagaaagcat taacagtaaa tccgaattct gaattaatct ttggatttaa tacaatgact
     1021 actaaaaacg gtcaaggcgc aacaaatgtt attatcaaaa atgctgatac taatgatacg
     1081 attgctgaaa agactgttga aggcggtcca actttgcgtt tatttaaagt acctgataat
     1141 gtgagaaatc tcaaaattca atttgtacct aaaaatgacg caataacaga tgcgcgtggc
     1201 atttatcaac taaaagatgg ttacaaatac tatagctttg ttgactctat cggacttcat
     1261 tctgggtcac atgtttttgt tgaaagacga acaatggatc caacagcaac aaataataaa
     1321 gagtttactg taacaacatc attaaagaat aatggtaatt ctggtgcttc tctagataca
     1381 aatgactttg tatatcaagt tcaattacct gaaggtgttg aatatgtgaa caattcattg
     1441 actaaagatt ttccaagtaa caattcaggc gttgatgtta atgatatgaa tgttacatat
     1501 gatgcagcaa atcgtgtgat aacaattaaa agtactggag gaggtacagc aaactctccg
     1561 gcacgactta tgcctgataa aatactcgat ttaagatata aattacgtgt aaataatgtg
     1621 ccgacaccaa gaacagtaac atttaacgag acattaacgt ataaaacata tacacaagat
     1681 ttcattaatt cagctgcaga aagtcatact gtaagtacaa atccatatac tatcgatatc
     1741 atcatgaata aagatgcatt acaagccgaa gttgacagac gtattcaaca agctgattat
     1801 acatttgcgt cattagatat ctttaatggt ctgaaacgac gcgcacaaac gattttagat
     1861 gaaaatcgta acaatgtacc attaaataaa agagtttctc aagcatatat tgattcatta
     1921 actaatcaaa tgcaacatac gttaattcga agtgttgatg ctgaaaatgc agttaataaa
     1981 aaagttgacc aaatggaaga tttagttaat caaaatgatg aattgacaga tgaagaaaaa
     2041 caagcagcaa tacaagttat cgaggaacat aaaaatgaaa taattggtaa tattggtgac
     2101 caaacgactg atgatggcgt tactagaatc aaagatcaag gtatacagac cttaagtggg
     2161 gatactgcaa caccggttgt taaaccaaat gctaaaaaag caatacgtga taaagcaacg
     2221 aaacaaaggg aaattatcaa tgcaacacca gatgctactg aagacgagat tcaagatgca
     2281 ctaaatcaat tagctacgga tgaaacagat gctattgata atgttacgaa tgctactaca
     2341 aatgctgacg ttgaaacagc taaaaataat ggcatcaata ctattggagc agttgttcct
     2401 caagtaactc ataaaaaagc tgcaagagat gcaattaacc aagcaacagc aacgaaaaga
     2461 caacaaataa atagtaatag agaagcaact caggaagaga aaaatgcagc attgaacgaa
     2521 ttaactcaag caaccaacca tgctttagaa caaatcaatc aagcaacaac aaatgctgat
     2581 gttgataacg ccaaaggaga tggtctaaat gccattaatc caattgctcc tgtaactgtt
     2641 gttaagcaag ctgcaaggga tgccgtatca catgatgcac aacaacatat cgcagagatc
     2701 aatgctaatc ctgatgcgac tcaagaagaa agacaagcag caattgacaa agtgaatgct
     2761 gctgtaactg cagcaaacac aaacatttta aacgctaata ccaatgctga tgttgaacaa
     2821 gtaaagacaa atgcgattca aggaatacaa gcaattacac cagctacaaa agtaaaaaca
     2881 gatgcaaaaa atgccatcga taaaagtgcg gaaacgcaac ataatacgat atttaataat
     2941 aatgatgcga cgctcgaaga acaacaagca gcacaacaat tacttgatca agctgtagcc
     3001 acagcgaagc aaaatattaa tgcagcagat acgaatcaag aagttgcaca agcaaaagat
     3061 cagggcacac aaaatatagt agtgattcaa ccggcaacac aagttaaaac ggatgctcgc
     3121 aatgttgtaa atgataaagc gcgagaggcg ataacaaata tcaatgctac aactggcgcg
     3181 actcgagaag agaaacaaga agcgataaat cgtgtcaata cacttaaaaa tagagcatta
     3241 actgatattg gtgtgacgtc tactactgcg atggtcaata gtattagaga cgatgcagtc
     3301 aatcaaatcg gcgcagttca accgcatgta acgaagaaac aaactgctac aggtgtatta
     3361 aatgatttag caactgctaa aaagcaagaa attaatcaaa acacaaatgc aacaactgaa
     3421 gaaaagcaag tggctttaaa tcaagtggat caagagttag caacggcaat taataatata
     3481 aatcaagctg atacaaatgc ggaagtagat caagcgcaac aattaggtac aaaagcaatt
     3541 aatgcgattc agccaaatat tgttaaaaaa cctgcagcat tagcacaaat caatcagcat
     3601 tataatgcta aattagctga aatcaatgct acaccagatg caacgaatga tgagaaaaat
     3661 gctgcgatca atactttaaa tcaagacaga caacaagcta ttgaaagtat taaacaagct
     3721 aacacaaatg cagaagtaga ccaagctgcg acagtagcag agaataatat cgatgctgtt
     3781 caagttgatg tagtaaaaaa acaagcagcg cgagataaaa tcactgctga agtggcgaag
     3841 cgtattgaag cggttaaaca aacacctaat gcaactgacg aagaaaagca ggctgctgtt
     3901 aatcaaatca atcaacttaa agatcaagca attaatcaaa ttaatcaaaa ccaaacaaat
     3961 gatcaggtag acacaactac aaatcaagcg gtaaatgcta tagataatgt tgaagctgaa
     4021 gtagtaatta aaccaaaggc aattgcagat attgaaaaag ctgttaaaga aaagcaacag
     4081 caaattgata atagtcttga ttcaacagat aatgagaaag aagttgcttc acaagcatta
     4141 gctaaagaaa aagaaaaagc acttgcagct attgaccaag ctcaaacgaa tagtcaggtg
     4201 aatcaagcag caacaaatgg tgtatcagcg attaaaatta ttcaacctga aacaaaagtt
     4261 aaaccagctg cacgtgaaaa aatcaatcaa aaagcgaatg aattacgtgc taagattaat
     4321 caggataaag aagcaacagc agaagaaaga caagtagcac tagataaaat caatgaattt
     4381 gtaaatcaag ccatgacaga tattacgaat aatagaacaa atcaacaagt tgatgataca
     4441 acaagtcaag cgcttgatag cattgcttta gtgacgcctg accatattgt tagagcagct
     4501 gctagagatg cagttaagca acaatatgaa gctaaaaagc gcgaaattga gcaagcggaa
     4561 catgcgactg atgaagaaaa acaagttgct ttaaatcaat tagcgaataa tgaaaaacgt
     4621 gcattacaaa acatcgatca agcaatagcg aataatgatg tgaaacgtgt tgaaacaaat
     4681 ggcattgcta cactaaaagg tgtacaacct catattgtaa ttaagcctga agcacaacaa
     4741 gcaataaaag caagtgcaga aaatcaagta gaatcaataa aagatacacc acatgcaaca
     4801 gttgatgaat tagatgaagc gaatcaatta attagcgaca cactcaaaca agcgcaacaa
     4861 gaaatagaaa atacaaatca agatgctgct gttactgatg ttagaaatca aacaatcaag
     4921 gcaatagagc aaataaaacc taaagtaaga cgtaaacgag ctgcgcttga tagcattgaa
     4981 gaaaataata aaaatcaact cgatgcaatc cgaaatacgt tggatactac tcaagatgaa
     5041 agagatgttg ctattgatac tttaaataaa attgtaaata caattaaaaa tgacattgca
     5101 caaaacaaaa cgaatgcaga agtggatcga actgagactg atggcaacga caacatcaaa
     5161 gtgattttac ctaaagttca agttaaacca gcagcgcgtc aatctgttgg tgtaaaagcc
     5221 gaagctcaaa atgcactaat cgatcaaagc gatttatcaa ctgaagaaga aagactagct
     5281 gctaaacatt tagtagaaca agcacttaat caggctattg atcagatcaa tcatgcagat
     5341 aagactgccc aagttaatca agatagtata aatgctcaaa atattatttc aaaaattaaa
     5401 ccagcgacaa cagttaaagc aacagcatta caacaaattc aaaatatcgc tacaaataaa
     5461 attaatttaa ttaaagcaaa taacgaagcg acagatgaag aacaaaatat tgcaatagca
     5521 caagttgaaa aagagttaat taaagctaaa caacaaattg ctagtgcagt gactaatgca
     5581 gatctggcat atttattgca tgatgagaaa aacgaaattc gtgaaatcga acctgttatt
     5641 aacagaaagg cgtctgctcg agaacaattg acaacattat tcaacgataa aaaacaagca
     5701 attgaagcga atattcaagc aacggtagaa gaaagaaata gtatattagc acagttacaa
     5761 aatatttatg acactgctat tggacaaatt gatcaagatc gtagcaatgc acaagttgat
     5821 aaaacagcat cattaaatct acaaacaata catgatttag atgtacatcc tattaaaaag
     5881 ccagatgctg aaaaaacgat taatgatgat cttgcacgcg tcactgcttt agtgcaaaat
     5941 tatcgaaaag taagtaatcg taataaggct gatgcattaa aagctataac tgctttaaaa
     6001 ttacaaatgg atgaagaatt aaaaacagca cgcactaatg ctgatgttga tgcagtttta
     6061 aaacgattta atgttgcatt aagcgatata gaagcagtaa ttactgaaaa agaaaatagc
     6121 ttactgcgaa ttgataacat tgctcaacaa acatatgcga aattcaaagc gatcgcaaca
     6181 ccagaacaat tagctaaagt aaaagtatta attgatcaat atgttgcaga tggcaataga
     6241 atgattgatg aagatgcgac attaaatgac atcaaacaac acacgcaatt cattgttgat
     6301 gaaattttag caattaaatt accagctgaa gcgacgaaag tatcaccaaa agaaattcag
     6361 ccagctccaa aagtttgtac gcctattaaa aaagaagaga cacatgaatc gcgcaaagtt
     6421 gaaaaagaac ttccaaatac aggttctgaa ggaatggatt taccattgaa agaatttgca
     6481 ctgattacag gtgcggcttt gttagctaga agacgtacta aaaacgaaaa agaatcataa
     6541 ttaacgaaat gaccttaata taacgacact ttttagtgac tcagttatat taaggtcatt
     6601 tttatttgaa tgaaaaagtt gatctactaa agagaatcga accaaatttt atactattat
     6661 tccattgatg catgtgagta atgtccggca tataatccag cgacatgtcc tgttacgagt
     6721 gcacttgtaa tattataacc accagtataa ccatgtatat ctaatacttc accacataaa
     6781 aataatcccg gaactaattt agacatcatt gttttaggtt gaatttcttt aagtgacaca
     6841 ccaccacctg tgacaaatgc cttatctata ggtaatgtcc cgttcacctt aaatacaaac
     6901 cctttaaaca tatttactaa gtcgttcaat tgttgatttg ataagtgatg tgatgtggta
     6961 ttttcatcga ttcctgcttg ttccagcatg aacagtaagt agcgctcttc aattagacca
     7021 tgcaaactgt ttttaatgat tttatctggt gtgtccgata ataatgatgt gatgtgttgt
     7081 tttaattgtt catggtttaa ttcaggaaat gcatcgattg ccatagaaat gtgctgtgtc
     7141 ttttgatttt tttgttcttt ataaacaaac tgactacatc ttaatgcagc tggaccactg
     7201 ataccaaaat gagtaaataa catatccatt tgatgactga tgcgtttttt accatttttc
     7261 ttaagtactg acaattcaac atcttttaaa cttaaacctt ttagacgatt ggatttgatg
     7321 aaaggttcag ctgatgtaat tggaacttcg gtcgggaata actcagtaat ggtatgacct
     7381 aaatcttgtg cgaacttata accatcacca gttgaaccag tttgagggac acttgtacca
     7441 cctgtagcaa tcactagcga atggctttca taactattat tttgagtatg tacagtgaaa
     7501 gtttggtctg tattaacttc gattctacta acagcttctt cttctttaat cgttacatgt
     7561 tggcgttcga tagttgtcac taatgtatca accacgtctt gtgctttgtt ggaaactgga
     7621 aacatacgcc cgtgatcttc ttcttttaat ttaacacccc tagactcaaa aaaatctatg
     7681 atggattcat tatcaaaaat tgaaaaggga ctatataaaa atttcccatt tccaggaatg
     7741 ttcttaataa tttcagcata tggtaatcga ttagttacgt tacatctacc gccaccagat
     7801 attttaagtt tacgacctag acctttcttt ttttcaatga gtaacacact gctactttgt
     7861 tcgcttgcag ctactgccgc cattaagccg ctaggtccgc ctccgataat aattgtttga
     7921 tacatatacg cttcctccaa atttgttact ggacattcaa ttgctatttt atagtacttt
     7981 tttaaacttt gctatatctc ttgtgttatg agatgacaat tatgttgaat atatttataa
     8041 tgtgaacttc ataaaaaatg aagatgtatg tgtatgataa gaatgatttt tctactgtta
     8101 tgcgatgttc atctcaaata gtagtataat gtttatatga aatttcgtaa tattctaatc
     8161 aaaatctagt attcaatgac aatgtgttga taattttgtt atgataattc attgaatata
     8221 cgacaatgaa ataaagtttt gtgatatatt cataataatg aaaaatgatt ttacagagaa
     8281 acagctttta gtttacaaaa ctttaataaa catgagatag ggagattcgt aatgagtgaa
     8341 agtaaagaaa tggtgcgtgg aaccttttta attaccatta gtatattaat taccaaggta
     8401 ttgggcgtac tttttatcat tccattcaac tatttaattg gtggacaaga aaatatggcg
     8461 ccgttcacat acgcttatgc accatataat attgcaattg cagttgcgac agcaggtgta
     8521 ccattagcag cttcgaaata tgttgcgaaa tataatgcta ttggggcata taaagtaagt
     8581 cagaaatttt ataaatcgag ttttattgta atgagtatta ccggtgtttt aggtttcttg
     8641 gtactgtatt tcttagcacc ttatatttct gaattaacat tagctagaaa cattcatgat
     8701 aaaaacggtt ggtcagtcga tgatattact tggattataa gaatcattag tatggttgtt
     8761 atctttattc ctgtattagc gacatggaga gggatattcc aaggttataa atctatgggg
     8821 ccaacagcag tgtcagaagt aactgagcaa attgcacgcg tgattttcat attaatcgga
     8881 agttatttag tactaaatgt ctttgatggc tctattttat tagccaatgg tattgctact
     8941 tttgcagcag cagttggtgc gattattggt atattcacac tctggtatta ctggagaaag
     9001 cgtaagcata atattgatcg tatggtcgaa tctgattaca cagatataga tgtgtcatat
     9061 ggcaagatgt ataaagaaat tattgcttat agtataccgt tcgttattgt aagtttgaat
     9121 tatccattat ttaacttagt agatcaattt acacataacg gtgctttatc tttagttggt
     9181 ataccttcac agttacaaga tatattcttt aatatgctaa atatgtcaac taataaaatt
     9241 gtaatgattc cgacatcatt aagtgctggt tttgcagtga gtttaattcc ttatattaca
     9301 aaaacatttg cagaaggtcg cttacatgaa atgcatcatc aaattagaac atctattggt
     9361 gtattaatgt ttataactgt tccagctagt attggaatta tggccttggc acaaccatta
     9421 ttcacagttt tctatggtta cgatccaatt gttttaggtc atgaccctaa ccatgatggt
     9481 agtcgcttat tattctatta tgcgcctgta gcaatactaa tttcactatt aagtgtaacg
     9541 gcatcaatgt tacaaggtat agataaacag aaattaacag tttatgttat tttagcttct
     9601 gtagttatca agttagcgtt aaactatcca ttaattatgt tattccatac acctggtgca
     9661 attttaagta ctagtatcgc attattattt gcaattggat gtaatttcta tattcttaaa
     9721 aaatatgcga agtttaaatt cagctatagt tggattcatt ttgcaaaaat attcttgtat
     9781 tcatttatta tgatgctagg tgtagaatta gtatttttcc ttgcaaatct attcttagaa
     9841 cctactaaac ttggttactt aatcattatt attttaggtg ttacagtagg tattctaatt
     9901 tacggtacaa tcacaattaa aacgcgtctt gcggacgaat tcttaggtga aattcctgaa
     9961 aaattaagac gtagagttag gtttttacga tgagaataga taaattttta gcaaatatgg
    10021 gcgtcggtac acgtaatgag gtcaaacagt tacttaaaaa aggccttgta aatgtcaacg
    10081 aacaagtaat taaatcacca aaaacacata tagaaccaga aaacgataag ataacagttc
    10141 gtggagaatt aatagaatac attgaaaatg tctatattat gttaaataag ccaaaaggtt
    10201 atatatcagc aactgaagat catcattcaa aaaccgttat tgatttaata cctgaatacc
    10261 aacatctaaa tatttttccg gttggtcgtc ttgataaaga tacagaaggg cttttattga
    10321 taacgaatga tggtgatttt aatcatgaat taatgagtcc aaacaaacat gtttctaaaa
    10381 aatatgaagt tatttcagca aatcctatca ctgaagacga cattcaagct tttaaagagg
    10441 gtgtaacact gactgatggc aaggttaagc ctgcaatatt aacttatatt gataatcaaa
    10501 cgtcacacgt aacaatttat gaaggaaaat atcatcaagt taaacgaatg tttcatagca
    10561 ttcaaaatga ggtattacat ttaagacgta tcaaaattgc tgatttagaa ttggacagca
    10621 atttagattc aggagaatat cgtttattaa ctgaaaatga ttttgacaaa ttaaattata
    10681 aataaaaggg agacgattga tatggcaaaa ggtaatttat ttaaagcgat tttaggtata
    10741 ggtggcgctg tagcagctgt acttgttaca cgtaaagata gtcgtgacaa gctgaaagca
    10801 gaatataata aatataaaca agatcctcaa agctataaag ataatgctaa ggataaagcg
    10861 acgcaattag gaacaattgc taatgaaaca attaaagaag taaaaacaaa tccgaaagaa
    10921 tatgctaata gattaaaaaa taatccaaaa gcatttttca aagaagaaaa atcaaaattt
    10981 accgaatatg acaataagaa tgacgaaagt attgaaaaag gtaaatttga tgatgaaggt
    11041 ggcgcagcac caaataataa tttacgtatc gtcactgaag aagatttaaa aaagaataaa
    11101 aatgcattat ctgataaaga ataagatgtg agtaggacag aaatgataag ggagtgggac
    11161 agaaatcata ttttagcaaa atttatttcg tcgtcccacc ccggcaaggt tgactagaat
    11221 tgaaaaaagc ttgttacaag cgtattttca ttcagtcaac tactgccaat ataatattgt
    11281 aaactatagg acatttatta gtgtttcagg ttctttatcg ttagtcatag ctaatatgca
    11341 tatagaaaga agaatacatg cgtgaaactc attcataaga aatactaata tctaaagaaa
    11401 acgtatttct ttatcttttc gaaattctct gtgttggggc ccttacccca acttgcacat
    11461 tattgaaagc tgactattgg ccagcttctg tgttggggcc ccgccaactt gcacattatt
    11521 gaaagctgac tattggccag cttctgtgtt ggggccctta ccccaacttg cacattattg
    11581 aaagctaact attggccagc ttctatgttg gggccccgcc aacttgcatt gtctgtagaa
    11641 tttcttttcg aaattctcta tgttggggcc ccggggcgca ttttcgttcg gttaattact
    11701 gtcaatatta aattgtgtgc ctagtacatt gacttatatc tcgagctctt gaatatgtgt
    11761 attttaaata tataaattaa aaaaggaaca aagcagctat ttatgatttg tgtcatatca
    11821 atagcatcgt tttgttcctt tttattattt tctgacatct tattgtagtt tttatatctt
    11881 ttcatgtaaa atagaattta acttatagaa aaaaggaagt cgatttcgaa tgtggaaaga
    11941 aaaagttcaa caatacgaag atcaaatcat taatgactta aaaggattat tagcaattga
    12001 aagtgtgaga gatgatgcaa aagcatcaga agacgcacca gttggtccag gtcctcgtaa
    12061 ggcattagac tacatgtatg aaattgcaca tagagatgga tttacaacac atgatgtgga
    12121 tcatattgca ggaagaattg aggcaggtaa aggaaatgac gtattaggta tcttatgtca
    12181 tgttgacgtt gttcctgctg gtgatggatg ggatagtaat ccgttcgagc cggttgtaac
    12241 agaagatgct atcatagcta gaggtacact tgatgacaaa ggtccaacaa ttgctgctta
    12301 ttatgcaatt aagatattag aagatatgaa tgtggattgg aagaaacgta ttcatatgat
    12361 tattggtacg gatgaagaat ctgattggaa atgtacggat cgctatttta aaacagaaga
    12421 aatgccaaca ttaggttttg caccagatgc agaatttcca tgtattcatg gtgaaaaagg
    12481 cattacaaca tttgatttag ttcaaaataa acttactgaa gatcaagatg aacctgatta
    12541 tgaattaata acttttaaat ctggtgaacg ttacaacatg gtacctgatc atgcagaagc
    12601 aagagtgctt gttaaagaaa atatgacaga tgttattcaa gactttgagt actttttaga
    12661 acaaaatcat ttacaaggtg atagtactgt tgatagtggc attctagttt taacagttga
    12721 aggtaaagcg gttcatggta tggatccatc tatcggtgtg aatgcgggtc tttacttact
    12781 aaaattctta gcatcattaa atcttgataa taatgcacaa gcgtttgtag catttagtaa
    12841 tcgctactta tttaattcag attttggtga aaagatggga atgaaattcc atacagatgt
    12901 catgggtgac gtgacaacta acattggtgt tattacatat gataatgaaa acgcaggtct
    12961 tttcggtatc aacttacgct acccagaagg atttgaattt gaaaaagcta tggatcgttt
    13021 tgcaaatgag attcaacaat atggctttga agtgaaatta ggtaaagtcc aaccaccaca
    13081 ttatgttgat aaaaatgatc cttttgtaca aaagttagtt actgcatata gaaatcaaac
    13141 aaatgatatg actgaacctt atactatagg tggcggtact tatgcgagaa acttagacaa
    13201 gggtgtagca tttggcgcaa tgtttagtga ttctgaagat ttaatgcatc agaaaaatga
    13261 atatatcact aaaaaacagt tatttaacgc aactagtatt tacttagaag caatttattc
    13321 attatgcgtg gaggaataat atatggaaaa aattttttta aatggtgagt ttgtaagtcc
    13381 aagtgaagca aaggtttcat acaacgacag aggatacgta tttggcgatg gtatttatga
    13441 atacattcga gtatataatg gtaagttatt tacagtaaca gaacattatg aaagattttt
    13501 acgtagtgcc aatgagattg gtttagattt aaattattct gtagaagaat taattgaact
    13561 atctcgtaaa ttagttgata tgaatcaaat tgaaactggg gcaatttata ttcaagcaac
    13621 gcgtggtgta gctgaaagga atcatagctt cccgacacct gaagtagaac cagcaattgt
    13681 tgcttataca aagagttatg atcgtcctta tgatcattta gaaaatggtg tgaatggtgt
    13741 taccgttgaa gatatccgat ggttacgttg cgacattaaa agcttgaact tattaggaaa
    13801 tgtattagca aaagaatatg ctgtgaaata taatgcagtt gaagcaattc aacatcgagg
    13861 tgaaactgta actgaaggat cttcaagtaa tgcttatgca attaaagacg gtgtgattta
    13921 tacacatccg attaacaact atattcttaa tggtattaca cgaattgtaa ttaaaaaaat
    13981 tgccgaagac tataacatcc catttaaaga agaaacgttt actgtagatt tcttgaaaaa
    14041 cgcagatgaa gttattgttt caagtacttc agctgaggtt acacctgtta ttaaattaga
    14101 tggtgaacca gttaatgatg gtaaagttgg cccaattaca cgtcaactac aagaaggatt
    14161 tgaaaagtat atagagtcac acagtattta aaattctttc atcatatttt tagattaaat
    14221 tacttaaatt tatcaacttt cgtaagtgaa ttatgttata atttaaaacg aagcttaaaa
    14281 gtgttgttta gaaaacacat attagtaaaa agtgccattt tgtttctcat tttcagttga
    14341 aatataatga taaaaattat aaaattttct atgagttaca aatatgatgt gcatcatatt
    14401 ttactaagta cgtaaatgtt acagttcata aatttatgct aaccatgaaa atgggatagc
    14461 aattattttg aaaactagag tttatcgcct acagagattc aaaaacatgt agttttagac
    14521 acggtaacat tatatgtcaa gtaagtgact atagaatgta tggtgtcaat gacagacatt
    14581 acgatttaaa tttagtcgtg taatgtaggc attggttgag gatttacaat aattatctgg
    14641 acataatatt tgtaccagta tggtgttata aattagaata tatggctctt gaaaaaccta
    14701 tttctagagc cattttctaa ttgaaagtga ggtggacgag ttggagcagt tttatcaatt
    14761 agggtggaca cttgattcag caggtggtgc atctggtgaa gcatatatgg ctgaacaaga
    14821 tggacaaaag ttgtttttaa aacgaaattc aaatccattt attgcggcat tatcagcaga
    14881 aggtattgtg cccaaattag tatggacgaa acgcatagaa acaggcgagg ttgttacagc
    14941 acaacattgg aaaaatgggc gtgaactatc ttcaaacgaa atgaagcaaa caagagttgc
    15001 acatttatta aagaagatac acaattctag acctttatta agtatgttaa agcgtatgga
    15061 aatggaacct attactcctg agattatgct taataaaatt aatgcctctt tatcaagaga
    15121 agttttaaca catcatattg tgagaaaatc attaacctat ttagaagagc atataccgag
    15181 tttagattcg cgttttttca ctgttgtaca tggtgatgtg aatcataata attggttatt
    15241 atccgatcga gatgaactat ttttagttga ttgggaaggt gcaatgattg cagacccggc
    15301 aattgatatc ggaatgctgc tttacaacta tgttccacaa caacaatggt ctgaatggct
    15361 tgaaacatat ggtgttcaag agagcttgaa tttaaataaa cgtatgaaat ggtatacggt
    15421 aattcaatct ataggtcttg ttcaatggta tgaagagcaa aaaagatata aggatatgaa
    15481 cacttggctt aaattcctaa atgaagttat gaatagtaac atgtttattt aagaggagaa
    15541 taaaatatga gagttcgata caaaccatgg gctgaagatt atttaaaaga tcatcctgaa
    15601 ttggttgaca tggacggtca acatgcaggg aaaatgactg aatggtttga taaaacgcaa
    15661 ccgatacaca ttgaaattgg ttcgggtatg gggcagttta ttacaacatt agctgctcaa
    15721 aatcctcata ttaactatat ttcaatggaa cgtgagaaaa gtatcgttta taaagtattg
    15781 gataaagtta aagagatggg tttaacaaat ttaaagatta tttgtaatga cgccattgaa
    15841 ttaaatgaat attttaaaga cggcgaagtt tcgcgtattt atttgaactt ttcagatcca
    15901 tggccaaaaa atcgtcatgc aaaacgacgt ttaacgtatc atacgttttt agcgttgtat
    15961 caacaaatct taaatgatga aggagattta cattttaaaa ctgataacag aggactattt
    16021 gcctacagtt tagaaagtat gtctcaattt ggaatgtatt ttacaaaaat aaatctaaat
    16081 ttacatcaag aagacgatgg cagtaatatt ttaacagaat atgaaaagaa attttcggat
    16141 aaagggtcac gtatttatcg tatggaagcg aaattccatt cacaaaaata gagcacttat
    16201 gagggtatgt acacatacaa tcataagtgt tttttcgtac tataatggtt ctatagataa
    16261 aggttctata ataaatcgga ctgatggaaa aagtttttaa cttttcatct gtccgatttt
    16321 ttgattttga atataaaaaa agcgcaatta tctctataat taaaagtgac taaacaaata
    16381 actaaaggag ataatgcg
//
LOCUS       DAXWFK010000048        15685 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.048, whole genome shotgun sequence.
ACCESSION   DAXWFK010000048 DAXWFK010000000
VERSION     DAXWFK010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15685
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.048"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(231..1109)
                     /locus_tag="ACGEXS_001186"
     CDS             complement(231..1109)
                     /locus_tag="ACGEXS_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HFF8012205.1"
                     /translation="MPNKILLVDGMALLFRHFYATSLHKQFMYNSQGEPTNGIQGFVR
                     HIFSAIHEIRPTHVAVCWDMGQSTFRNDMFDGYKQNRSAPPEELIPQFDYVKEISEQF
                     GFVNIGVKNYEADDVIGTLAQQYSTDNDVYIITGDKDLLQCINDNVEVWLIKKGFNIY
                     NRYTLHRFNEEYALEPQQLIDIKAFMGDTADGYAGVKGIGEKTAIKLIQQYQSVENVV
                     ENIDALSAGQRNKINDNLDELYLSKRLAEIHTQVPIDSEALFEKMSFATTLNHILSIC
                     NEHELHVSGKYISSHF"
     gene            complement(1129..4569)
                     /locus_tag="ACGEXS_001187"
     CDS             complement(1129..4569)
                     /locus_tag="ACGEXS_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HFF8012206.1"
                     /translation="MINKEQLDLLYKLKKEVEKSRNEALLHTINQVIKKVYLQQYTCS
                     FVGHFSAGKSTLINLLIEQDILPSSPVPTTSNTAIVSVSDNHDIIANLPNQTYAKLSN
                     YDEVREMNRQNVDVESVEINFQSAKFENGFTLQDTPGVDSNVASHQSITEQYMYTSNM
                     IFYTVDYNHVQSELNFKFMKHINDVGIPVVFIINQIDKHQDDELSFSTFKSRVEKSIA
                     DWGIKLERTFYVSKFDHPENELEVLSSYLISLDQHRETIEDYTSRTVEYITEAQLDYI
                     QSEIQEVLEDLGIEEAEFEQAFLNSQQHQAISEEAQLLNNPDELMAFLKNKRKNILEN
                     AYIMPHNMREMLRSYLESMSQDFNVGGFFNKKKKKLQIQQQRLLTATDALQEHVNQQI
                     RQPMREDMSFVTRFINKKEASDKVLNQHYDVKPEMIEGLYQPQTSISNTYVLTFSDEV
                     VKAIKKYVEQQSTPIFKEIIENVQADELPTEESDDLKEYQRYTELNELRQSLTTKNYR
                     HYYIHLDESLDKLIGRQETTYQVATDNAQDNRDNQQLNQNTATTNMSIDIQKALDIIS
                     DVPLFKRTKQDIHETLTRIDNKLIKIGVFGTFSAGKSSLINALLGEHILVSSPNPTTA
                     ATTEISYGDESYITLKSQSQLLDEINAVVEYQDMSYSTIEDFINSDLEKLKSHLNKNQ
                     LAFIQAVEKHYKLYVNMLENGEKHAINQQELKKWSAEDEYATFVKTVHIALMHDWLKG
                     KIIVDSLGLHSNNQRHTNETEQILTSSDLILYVSYFNHSFTDNDKAFIEHMKDMNQLN
                     ENQAFKMVINAADLAESQDDLEAVETYVSDALRQVHLQSDIFAVSSRNALQAEDKGID
                     QLKQSIQQFVDVESKSILEQQMIHQLQQMDRSYVEMITEFETNKADISRRQQRLTDYK
                     DKQRLQHQLIDATLQHTDNEVEEQVYHLNARLKLQLLDDVKSVFNSQMTQNSDFNEEK
                     KVSTRVYLDQIHQRLFLEQSLITERIKKYFNKQLTEQIAPIVQQLADLHVIINPQFNF
                     ESANIEQPLLHIDFNDMLNALPKQLTKRKILNPNGQRDIHESICQSTLGLLQPQMGLL
                     RQQLELYVKQMAVEAESQFESFEANIQTQINDLLAFDLDTTLINQLKDKHQQLKTILY
                     "
     gene            4873..6213
                     /locus_tag="ACGEXS_001188"
     CDS             4873..6213
                     /locus_tag="ACGEXS_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HFF8012207.1"
                     /translation="MKNTFNPLQRLHFYAAIFIAPLLITLTISGIGYLFFPEVENNIY
                     KNEFFGDSDVKTHQTLNDAVHQVEQQYEGFFVSKVSILDEPYNKRITLSDMAGNQRYV
                     FLDHNNQFVADQNAKHTYSNVMRSIHSSLFTENTIINYLVELTACWMIFMILSGTYLL
                     IKKHLISNKSKALRWQKWHAMIGVIIAIPVFVLVLTGLPWSGFMGSKIAGMMDTNGDL
                     GQGELAINPPKSDLNELPWATRKNKQPASSEKGSSGHHGNAAMPQTKLDYQISIDKVV
                     EQAQKAGIKKPFSIVYPSDKNGTFIVSNTSNSGVTGLDVSPYKEQTLYFDQYSGKKLG
                     TIKYDDYGIIAKWFTWGIPLHEGHLFGILNKIINLFVCIALLVAIGMGFVSWIKRTKN
                     TAVKVPHRVKKPASISLIICLIVLGLLMPLFGLSLILVFIIELILYIKDRRAKQ"
     gene            complement(6419..6751)
                     /locus_tag="ACGEXS_001189"
     CDS             complement(6419..6751)
                     /locus_tag="ACGEXS_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4889 domain-containing protein"
                     /protein_id="HFF8012208.1"
                     /translation="MGKKMGLGLSIALVVIGIAVVCLMIFSSQKTTYFGYMNSNTNAE
                     KVVSEKDGLVKHNIKVEPSNDFKPKKGDFVKLVSKDDGKTFYKQEIVKHDDVPHGLMM
                     KIHDMHMN"
     gene            complement(6835..7980)
                     /locus_tag="ACGEXS_001190"
     CDS             complement(6835..7980)
                     /locus_tag="ACGEXS_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFF8012209.1"
                     /translation="MFQLLAVCPMGLEAVVAREIQELGYETNVENGRIFFEGDASAIV
                     KANLWLRTADRIKIVVGRFNATTFDELFEQTKALPWESIIDKEGNFPVQGRSVKSTLH
                     SVPDCQAITKKAIVERLRRAYNEKGWLNESGAKYPVEVAILKDNVLLTIDTSGSGLNR
                     RGYRLAQGEAPIKETLAASLIRLANWKGDTPLIDPFCGSGTIAIEACLIAQNIAPGFN
                     REFVSEQWNIMPANIYDDYRDEADKMADYDKEIEVYASDIDPEMVEIAKRNAEEVGLS
                     DIIKFSVKDVNTLTIDTEEPVALIGNPPYGERIGDREEVEEMYRYIGKLMKQHPFLST
                     YILTSNKEFEYLVDRKATKRRKLFNGYIECTYYQYWGKKTERKTIEN"
     gene            complement(8189..8568)
                     /gene="rnpB"
                     /locus_tag="ACGEXS_001191"
     ncRNA           complement(8189..8568)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACGEXS_001191"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            complement(8631..8975)
                     /gene="gpsB"
                     /locus_tag="ACGEXS_001192"
     CDS             complement(8631..8975)
                     /gene="gpsB"
                     /locus_tag="ACGEXS_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFF8012210.1"
                     /translation="MSDVSLKLSAKDIYEKDFEKTMARGYRREEVDAFLDDIIADYQK
                     MADMNNEVVKLSEENHKLKKELEELRLRVATSRPQDNKSFSSNNTTTNTSSNNVDILK
                     RISNLEKAVFGK"
     gene            complement(8989..9552)
                     /locus_tag="ACGEXS_001193"
     CDS             complement(8989..9552)
                     /locus_tag="ACGEXS_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFF8012211.1"
                     /translation="MVKTVYVTGYKSFELNIFKDDAPEVHYLKQFIKHKIEQLLDEGL
                     EWVLIQGQMGIELWTAEVVIELQRTYDSLKFAVITPFQGHTEKWNEHNQSKYANIIKH
                     ADYVDSIFHTSYQGPFQFKQADQFMLEHSDQTLLIYDEEQEASPKFFKQMLVDFMDKT
                     NYTCDIVTFDELTAFINDLQWSEDQSF"
     gene            complement(9545..9895)
                     /locus_tag="ACGEXS_001194"
     CDS             complement(9545..9895)
                     /locus_tag="ACGEXS_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YppE family protein"
                     /protein_id="HFF8012212.1"
                     /translation="MNDVVESLIYEVNNMQQNFENVKSQQQDHDFYQTVKPYTEHIDS
                     ILNEIKLHREFIIEVPYMNSRKFELLIANIEQLSVECHFKRTSRKLFIEKLKSVQYDL
                     QNILDGVTKEGTDG"
     gene            complement(9950..10129)
                     /locus_tag="ACGEXS_001195"
     CDS             complement(9950..10129)
                     /locus_tag="ACGEXS_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001788819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012213.1"
                     /translation="MLLISRGVFDLNDFIRLKVFSSYNNIHDKILYLYRKFLDLLDLT
                     DNTFEKIVKSNYIEC"
     gene            10435..11061
                     /gene="recU"
                     /locus_tag="ACGEXS_001196"
     CDS             10435..11061
                     /gene="recU"
                     /locus_tag="ACGEXS_001196"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFF8012214.1"
                     /translation="MNYPNGKPYRKNSAIDGGKKTAAFSNIEYGGRGMSLEKDIEHSN
                     TFYLKSDIAVIHKKPTPVQIVNVNYPKRSKAVINEAYFRTPSTTDYNGVYQGYYIDFE
                     AKETKNKTSFPLNNIHDHQVEHMKNAYQQKGIVFLMIRFKTLDEVYLLPYSKFEVFWK
                     RYKDNIKKSITVDEIRKNGYHIPYQYQPRLDYLKAVDKLILDESEDRV"
     gene            11058..13241
                     /locus_tag="ACGEXS_001197"
     CDS             11058..13241
                     /locus_tag="ACGEXS_001197"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFF8012215.1"
                     /translation="MTENKGSSQPKKNGNNGGKSNSKKNRNVKRTIIKIIGFMIIAFF
                     VVLLLGILLFAYYAWKAPAFTEAKLQDPIPAKIYDKNGELVKTLDNGQRHEHVNLKDV
                     PKSMKDAVLATEDNRFYEHGALDYKRLFGAIGKNLTGGFGSEGASTLTQQVVKDAFLS
                     QHKSIGRKAQEAYLSYRLEQEYSKDDIFQVYLNKIYYSDGVTGIKAAAKYYFNKDLKD
                     LNLAEEAYLAGLPQVPNNYNIYDHPKAAEDRKNTVLYLMHYHKRITDKQWEDAKKIDL
                     KANLVNRTPEERQNIDTNQDSEYNSYVNFVKSELMNNKAFKDENLGNVLQSGIKIYTN
                     MDKDVQKTLQNDVDNGSFYKNKDQQVGATILDSKTGGLVAISGGRDFKDVVNRNQATD
                     PHPTGSSLKPFLAYGPAIENMKWATNHAIQDESSYQVDGSTFRNYDTKSHGTVSIYDA
                     LRQSFNIPALKAWQSVKQNAGNDAPKKFAAKLGLNYEGDIGPSEVLGGSASEFSPTQL
                     ASAFAAIANGGTYNNAHSIQKVVTRDGETIEYDHTSHKAMSDYTAYMLAEMLKGTFKP
                     YGSAYGHGVSGVNMGAKTGTGTYGAETYSQYNLPDNAAKDVWINGFTPQYTMSVWMGF
                     SKVKQYGENSFVGHSQQEYPQFLYENVMSKISSRDGEDFKRPSSVSGSIPSINVSGSQ
                     DNNTTNRSTHGGSDTSANSSGTAQSNNNTRSQQSRNSGGLTGIFN"
     gene            complement(13807..14148)
                     /locus_tag="ACGEXS_001198"
     CDS             complement(13807..14148)
                     /locus_tag="ACGEXS_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpoC family protein"
                     /protein_id="HFF8012216.1"
                     /translation="MTNITKEVFDNLEQEIDLFAKNKTLGSSEAKPYLDEYHSKIIDY
                     FKQVNDITGNIDFDKLNQYPVVPMNFKERYDYMIERKYHFMGYRQMKTFKTELIKMNA
                     SYQTRLKNKQV"
     gene            complement(14153..14812)
                     /gene="nth"
                     /locus_tag="ACGEXS_001199"
     CDS             complement(14153..14812)
                     /gene="nth"
                     /locus_tag="ACGEXS_001199"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFF8012217.1"
                     /translation="MVSKKKALEMIDVIANMFPDAECELKHDNPFELTIAVLLSAQCT
                     DVLVNRVTTELFKKYKTPEDYLAVSDEELMNDIRSIGLYRNKAKNIKKLCQSLIDQFN
                     GEIPQTHKELESLAGVGRKTANVVMSVAFDEPSLAVDTHVERVSKRLGINRWKDNVRQ
                     VEDRLCSVIPRDRWNRSHHQLIFFGRYHCLARKPKCDICPLLEDCREGQKRYKASLKE
                     A"
     gene            complement(14802..15488)
                     /locus_tag="ACGEXS_001200"
     CDS             complement(14802..15488)
                     /locus_tag="ACGEXS_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HFF8012218.1"
                     /translation="MDKYQLKARPVVIRRELLDHYSDLGLDEQDLVILLKLIYASETS
                     NKQPSIELLQKGSTMQPRDITMVIQNLIQRELLELQVQKDEEGRFTEYMNLDPFFEKL
                     SHILKQQSMETKELNSKEKFKQLFRVLEDTFARPLSPYEIETLNQWIDVDKHDTAIIQ
                     AALDEANSLNKLSFKYIDRILLNWKKNNVKTIDDSRKIREKFNKPKMTHTVKTVPKFD
                     WLNGENLDGK"
BASE COUNT         5040 a         2661 c         2289 g         5695 t
ORIGIN      
        1 ttagattaat aaagttgtaa ctgtactatt ttgtagtgta tggtaattta tttggatgaa
       61 atataattct ttatactcta ttgattaatc aaatatgtat ctatcaaaat tcggcttatt
      121 tattcactct gacatatcca aatacgcaaa aagactattt ctaccttgta tcgatagaaa
      181 tagtcttttt atacttaatt ggtttaattt ttcataaaat cttatacttt ttaaaaatgg
      241 gatgaaatat attttcctga aacatgtaat tcatgttcgt tacaaattga taaaatatga
      301 ttaagcgttg ttgcaaatga cattttctca aaaagcgctt cactatcaat tggtacttgt
      361 gtgtgaattt cagccagtcg cttcgataaa tatagttcat ctaaattgtc attaatctta
      421 ttacgttgcc cagctgataa tgcatcaatg ttttcaacca cattttcaac gctttgatat
      481 tgctgaatta acttaatagc cgttttctcg ccaatacctt taacacccgc atatccatct
      541 gctgtatcac ccataaacgc tttaatatcg attagttgtt gtggttcaag ggcatattct
      601 tcgttaaaac gatgtaatgt atatctatta taaatgttaa aacctttttt aattagccaa
      661 acttcaacat tgtcattaat acattgcagt aaatctttgt cgcccgtaat aatatagaca
      721 tcgttatcag ttgaatattg ttgtgctaat gtacctataa catcatccgc ttcatagttt
      781 ttaacgccaa tatttacaaa gccaaattgc tctgaaattt ctttaacata atcaaattgt
      841 ggtatcaatt cttctggtgg tgcagaacga ttttgcttat aaccatcaaa catatcattt
      901 ctaaaagttg attgtcccat atcccaacat acagctacat gtgtagggcg tatttcatgt
      961 attgccgaaa agatatgacg cacaaatcct tgtattccat ttgtaggttc accttgtgaa
     1021 ttgtacataa attgtttatg aagacttgta gcatagaaat gtctaaataa tagcgccata
     1081 ccatctacaa gtaatatttt attaggcatt taaaacgttc cttctttctt aatataaaat
     1141 agttttcagt tgttgatgtt tatctttcaa ttgattgata agtgttgtat ctaaatcaaa
     1201 tgctaataaa tcgtttattt gcgtttgaat attagcttca aaactttcaa attgcgattc
     1261 agcttctaca gccatttgct ttacatataa ttcaagctgt tgcctcaata atcccatttg
     1321 tggttgtaat aatcctaacg tactttgaca aattgattca tgtatatctc tttgcccatt
     1381 tggattcaaa attttacgtt ttgttaattg tttaggcaat gcatttagca tatcgttgaa
     1441 atcgatgtgc aataatggtt gctctatatt agctgattca aagttaaact gaggattaat
     1501 aatgacatgt aaatctgcta attgttgaac gattggtgca atttgctcag tgagttgctt
     1561 attaaagtat ttttttatac gttctgtaat taaagattgc tctaaaaaca atcgttgatg
     1621 aatttgatct aagtatactc tcgtagacac tttcttttct tcattaaaat cactattttg
     1681 cgtcatttga gaattaaaca ctgatttaac atcgtcaagt agttgtagtt ttaaacgggc
     1741 atttaaatga taaacttgtt cttcaacttc gttgtctgta tgttgtaacg tcgcatcaat
     1801 taattgatgt tgtaaacgct gtttatcttt ataatctgtt aacctttgtt ggcgacgtga
     1861 aatatcagct ttatttgttt caaattctgt aatcatctct acataagaac gatccatttg
     1921 ttgaagctga tgaatcattt gttgttctaa aattgattta gattcaacat caacaaattg
     1981 ttgtatgctt tgttttaatt gatcaatgcc cttatcttca gcttgcaatg catttcgact
     2041 tgatacagca aaaatgtctg attgtaagtg tacttgtctt aatgcatctg atacatatgt
     2101 ttcaactgct tcaagatcat cttgactttc tgctaaatca gcagcattaa ttaccatttt
     2161 aaatgcttgg ttttcattca actggttcat atctttcatg tgttctataa acgctttgtc
     2221 attatcagta aatgaatgat taaaataact tacatacaat attaagtctg aagaagttaa
     2281 aatttgttcg gtttcatttg tatgcctttg gttatttgag tgtagcccta atgaatcaac
     2341 aattatttta ccttttaacc aatcatgcat taatgcaatg tgtactgtct taacaaatgt
     2401 tgcatattca tcttctgcac tccacttttt caattcctgt tgattaatgg catgtttttc
     2461 tccattttcc aacatattga catacaattt ataatgtttt tcaactgcct gaataaaagc
     2521 gagttgattt ttatttaaat gtgattttaa cttttctaaa tctgaattaa taaagtcttc
     2581 tatagtcgaa taagacatat cttggtattc aactactgca ttaatttcat ctaataattg
     2641 cgattgtgat tttagcgtta tataactctc gtctccataa gatatttctg tagtagctgc
     2701 tgtagtagga tttggagaac tgactaaaat atgctcgcct aataatgcat ttatcaaact
     2761 acttttacca gcactaaatg ttccaaatac acctattttt attaatttat tatctatacg
     2821 tgttaatgtt tcgtggatat cttgctttgt acgcttgaac aaaggcacat ccgaaattat
     2881 atcaagcgct ttttgaatat ctatagacat atttgttgta gctgtatttt gatttagctg
     2941 ctgattatca cgattatcct gagcattatc agtagccact tgatatgttg tctcttgtcg
     3001 acctattaat ttatctagag attcatctaa atgaatatag tagtgacgat aattcttagt
     3061 cgtcaatgac tgacgcagct catttaattc tgtataacgt tgatattctt ttaaatcatc
     3121 actttcttct gttggtaatt catctgcctg cacattttct attatttctt taaaaattgg
     3181 tgttgattgt tgttcaacat atttcttaat ggctttaacc acttcgtctg aaaatgtaag
     3241 tacataagta ttgctgattg atgtttgtgg ttgatataaa ccttcaatca tttctggctt
     3301 aacgtcataa tgctgattta atactttatc tgaagcttct tttttattga taaaacgcgt
     3361 aacaaatgac atatcttctc gcattggttg acgaatttgt tgattaacat gttcttgtaa
     3421 cgcatctgtc gctgttaata atcgctgttg ttgaatttgt agttttttct tctttttatt
     3481 aaaaaatccg ccaacattaa agtcttgaga catactttcc aaataacttc gtaacatttc
     3541 tctcatatta tgcggcataa tgtatgcatt ttctaaaata tttttacgct tattctttaa
     3601 aaatgccatt aattcatctg gattatttaa aagttgtgcc tcttcactaa ttgcttgatg
     3661 ttgttgacta tttaaaaatg cttgttcaaa ctccgcttct tcgataccta aatcttctag
     3721 tacttcctga atttcagact gaatgtaatc tagctgagct tcggtaatgt attcaaccgt
     3781 tcttgatgta taatcctcta ttgtctctct atgttgatct aatgaaatta gataacttga
     3841 taatacttca agttcatttt cagggtgatc aaatttagat acataaaagg tgcgttctaa
     3901 tttaataccc caatctgcaa ttgatttttc aactcgagat ttaaacgtag agaatgacaa
     3961 ttcatcgtct tgatgcttgt caatttgatt aatgataaac acaacaggta ttccaacatc
     4021 atttatatgc ttcataaact taaagttaag ttcagattga acgtggttat agtcaaccgt
     4081 ataaaatatc atattacttg tatacatata ttgttctgtt attgactgat gtgatgcaac
     4141 atttgaatca acacctggtg tatcttgcaa cgtaaaccca ttttcaaatt tagctgattg
     4201 aaaattaatt tctacagatt caacgtcgac attttggcga ttcatttccc ttacttcatc
     4261 ataattagat aatttggcat acgtttgatt cggcaaatta gcaataatat cgtgattgtc
     4321 tgaaactgac acaatagcag tattacttgt cgttggtaca ggtgaacttg gtaagatatc
     4381 ttgttcaatt aataaattta tcagtgtcga tttacctgca gaaaaatgtc caacgaacga
     4441 acatgtatat tgctgcaaat atactttctt aattacttgg ttaattgtat gtaaaagtgc
     4501 ttcatttcgc gacttttcaa cttctttttt taatttatat aaaagatcta attgttcttt
     4561 attaatcatg ttactttccc ctttggttcg ttcacataga aatgcttgct tgttacaacg
     4621 aattcataca atatcgatgt ccatttaaac atagatcgtt aaatggctcg cattgtatta
     4681 tgtttaaatt ttatcatagc tgcttgataa aacatacgaa taatcaattt tcttcaaaca
     4741 ttccaactta aaagttcatt tttctaaaaa tcacaaatca tcgttaaatt tttctagaaa
     4801 ataaatattg tcacagtttg ttcacacaca tcacagaatt gacaccgtat aatagccaat
     4861 tgggagggaa atatgaaaaa tacatttaat ccattacaaa gattacattt ctatgcagca
     4921 atatttattg ctccactgtt aatcacttta accatttcgg gcattggtta cttgttcttt
     4981 ccagaagttg aaaataatat ttataagaat gagttttttg gtgacagtga tgtaaaaacg
     5041 catcaaacat taaatgatgc agtacatcaa gttgaacaac aatatgaagg attctttgta
     5101 agtaaagtta gcatacttga tgaaccatat aacaaacgaa ttacactgag tgatatggca
     5161 ggaaatcaac gttacgtctt tctagatcat aacaatcaat ttgttgcaga tcaaaatgcg
     5221 aaacatacgt attctaatgt gatgcgaagt atacatagtt ctttgtttac tgaaaatact
     5281 attattaatt atttagtaga gttaaccgca tgttggatga tattcatgat tttatctggt
     5341 acttatttac tcattaagaa gcatttaatt tctaacaaaa gtaaggcact tcgttggcaa
     5401 aagtggcacg caatgattgg agttatcatt gcaattccag tatttgtatt agtcttaact
     5461 ggattgccat ggtctggttt tatgggcagt aaaattgccg gtatgatgga cacaaacggt
     5521 gaccttggtc aaggtgaatt agcgattaat ccacctaaat cagatttgaa cgaattacct
     5581 tgggctacac gtaaaaataa acagccagct tcatccgaaa aaggttcaag tggtcatcat
     5641 ggtaatgcag caatgcctca aaccaaatta gattatcaaa tatctattga taaggtcgtt
     5701 gaacaggcgc aaaaagctgg tattaaaaag ccgttttcaa tcgtatatcc aagtgataaa
     5761 aatggtacct ttattgtatc taatactagt aattcaggtg ttactgggct agatgtatca
     5821 ccatacaagg aacaaacact ttatttcgat caatatagcg gtaaaaagct aggtacgatt
     5881 aaatatgatg actacggtat tattgctaaa tggtttacat ggggcattcc gcttcacgaa
     5941 ggtcatttat tcggcatttt aaataaaatc attaatttat ttgtatgtat cgctttatta
     6001 gtagccattg gcatggggtt tgtctcttgg ataaagcgta caaaaaatac tgcagtaaaa
     6061 gtaccacatc gcgtaaaaaa accagcatct atatcactca taatatgttt aattgtatta
     6121 ggattattaa tgccattatt tggattatca cttatccttg tatttataat tgaattaata
     6181 ttatatatta aagatcgtcg tgctaaacaa taatgcactt aaagttttga actgacgaaa
     6241 tttacaaaat gattctcgtc tctctaatta cttaaaacgg gttctataat aaatcgtact
     6301 gatggaaagt ttttactttt tatctgtccg attttttgaa tttgaagata aaaaagcatc
     6361 taaaacgcat aagaagataa ctctacttct tcaaaattac gttatagatg cttttttatt
     6421 aattcatatg catgtcgtga attttcatca ttaaaccgtg tgggacgtca tcatgtttaa
     6481 caatctcttg tttataaaat gtcttcccat catctttaga aactaatttt acaaagtctc
     6541 cttttttcgg cttgaaatca ttagatggtt ctactttgat attatgtttg actaatccat
     6601 ctttttcact gacaactttt tctgcatttg tattactatt catataacca aaataagtcg
     6661 ttttttgact agaaaaaatc attaaacata caacggcaat accaataaca accaatgcaa
     6721 tagataaacc tagacccatt tttttaccca tttatagact cctccatttt tcaaaaatac
     6781 ctattatcat gagaacttaa ttattactaa ttttagtcac gcattagaaa cactttagtt
     6841 ttcaatagtt ttacgttctg tctttttacc ccagtattga taataagtac attctatata
     6901 tccattaaat aacttacgtc gctttgttgc tttacgatct actaaatatt caaattcttt
     6961 attacttgtt aaaatgtatg tagataaaaa tggatgttgt ttcattagtt taccaatata
     7021 acggtacatt tcttcaactt cttcacgatc accaatacgt tcaccatatg gaggatttcc
     7081 aattaacgcc accggttctt ctgtatcaat tgttaatgta ttgacatctt ttacactaaa
     7141 tttaataata tcagacaacc caacttcttc agcgttacgc ttagcaatct ctaccatttc
     7201 tggatcgata tcagaagcat atacttcgat ttctttatca taatcagcca tcttatccgc
     7261 ttcatcacgg taatcatcat aaatatttgc tggcatgatg ttccattgct ctgatacgaa
     7321 ctcgcgatta aaaccaggtg cgatattttg agcaattaaa caagcttcta tagctattgt
     7381 acccgaaccg caaaatggat caattaaagg tgtatcacct ttccagtttg caagacggat
     7441 taaacttgct gccaacgttt ctttaattgg tgcttcacct tgtgctaatc tataaccacg
     7501 tctgttcaaa ccagaacctg atgtgtcgat agtcaataat acattatctt ttaaaatggc
     7561 aacttcaaca gggtatttgg cacctgattc atttaaccaa cctttttcgt tatatgcgcg
     7621 acgtaatcgt tcaacaatag ctttcttagt tatcgcctga caatctggca cactatgtag
     7681 tgttgattta acgcttctac cttgaactgg gaagttaccc tctttatcaa ttatagattc
     7741 ccaagggagc gctttggttt gttcgaataa ttcgtcaaac gttgttgcgt taaaacgtcc
     7801 aacaacaatt ttgattcggt ctgctgtgcg caaccataaa tttgccttta caattgcact
     7861 tgcgtctcct tcaaaaaata tacgaccatt ttcaacattt gtttcatagc ctaattcttg
     7921 aatttcccta gcaacaacag cttctaatcc catcggacaa actgcaagta attgaaacat
     7981 atatgattct ccttttatac aggtatttta ttcttagctt gtgtttttta tacatttcca
     8041 acaaatttaa tcgctgatac attaacgcat ccgcttacta ttttaaaaca aggcagtgtc
     8101 attatatcaa gacaaggcgt taattttaag tgttcttttc atgaaaaaag ctctccatca
     8161 tctaggagag ctaaactagt agtgatattt ctataagcca tgttctgttc catcgtactc
     8221 atcacgtgca ctagtcacac tggtactcag gtgataacca tctgtctaca ccacttcatt
     8281 tcgcgaagtg tgtctcgttt atacgttgaa ttccgttaaa caagtgctcc taccaaattt
     8341 ggattgctca ctcgaggggt ttaccgcgtt ccacctttta tatttctata aaagctacgt
     8401 cactgtggca ctttcaaatt actctatcca tatcgaaaga cttaggatat ttcattgccg
     8461 tcaaattaat gccttgattt attgtttcat caagcacgaa cactacaatc atctcagact
     8521 gtgtgagcat ggactttcct ctatataata tagcgattac ccaaaatatc acttttaaaa
     8581 ttataacata gtcattatta gtaagacagt taaacttttg tatttagtaa ttatttacca
     8641 aatacagctt tttctaagtt tgaaatacgt tttaaaatat ctacattatt tgaagatgta
     8701 tttgttgttg tattattcga agaaaaactt ttattgtcct gaggtcttga tgttgctaca
     8761 cgtagtctta attcttctaa ttctttttta agtttatgat tctcttctga taattttaca
     8821 acttcattat tcatatcggc cattttttga taatcagcaa taatgtcatc taaaaatgca
     8881 tctacttctt ctcttctata gccacgagcc atcgtttttt caaaatcttt ttcataaata
     8941 tcttttgctg ataatttcaa tgaaacatct gacatttttt ccacctcatt agaaactttg
     9001 atcttcagac cactgtaagt cattgatgaa tgctgttaat tcatcgaacg tcacaatatc
     9061 acaagtatag tttgttttat ccataaaatc aactaacatc tgcttgaaga acttaggact
     9121 cgcctcttgt tcctcatcat aaatgagcaa tgtttgatcc gaatgttcaa gcataaattg
     9181 atctgcttgt ttaaattgaa aaggaccttg atacgacgta tgaaaaatac tatcaacata
     9241 atctgcatgc ttaattatgt tggcatactt actttgatta tgttcattcc acttttctgt
     9301 atgtccttgg aacggtgtaa ttacagcaaa ctttaacgaa tcatatgttc gttgtaattc
     9361 aataacaact tctgcagtcc ataattctat acccatttgc ccttgtatta acacccattc
     9421 taatccttca tccaacagtt gttcaatttt atgttttata aattgtttta aataatgtac
     9481 ttcaggtgcg tcatctttaa aaatgtttaa ttcgaatgat ttgtaacctg ttacataaac
     9541 tgttttaacc atcagtaccc tcttttgtta cgccatctaa tatattttgt aaatcatatt
     9601 gaacactttt aagcttttct ataaataact ttcgacttgt tcgcttaaaa tgacattcga
     9661 cagaaagttg ttcaatgtta gcaatcagta gctcaaattt ccttgaattc atataaggta
     9721 cttctataat aaattcacga tgtaatttga tctcattgag tatgctatca atatgttcag
     9781 tatatggctt tacagtttgg taaaaatcat gatcttgttg ttgtgatttc acattttcaa
     9841 aattttgttg catgttgtta acttcataaa ttagtgattc gactacatca ttcatgagga
     9901 cgcctcctac attttttaat ttatcacaat atactgtatt cgtcatgttt taacactcta
     9961 tataatttga tttaactatt ttttcaaatg tgttatctgt taaatcaagt aaatctaaaa
    10021 acttcctata taaatacaaa attttatcgt gtatgttgtt atacgatgaa aatactttta
    10081 atctaataaa atcatttaaa tcaaatacac ctctgctgat taacaacaca tacttgtact
    10141 tgcctcaaaa ataaaaatta ctaatcatga tttgactttt ataacaaaat tcaaaaatat
    10201 tgtaatgagt attcattttt ataagcaaaa cttttcaatt aattaaaatt gtgtgtaaaa
    10261 ttccaacatt aacggtaaat gtattataat attattgtgt taatgctgaa atcacgttaa
    10321 aataagcttt ggtgtattgt gtctttcgca tttatactaa tttaagtttg gtgtttcact
    10381 ataatgttgt gtataataaa caacgtttac taccaaactt gtgtggtgaa atttatgaat
    10441 tatccaaatg gtaaaccata tcgtaaaaat agtgctatag acggagggaa aaagaccgct
    10501 gcctttagta atattgagta tggtggacgt ggtatgtcac ttgaaaaaga tatcgaacat
    10561 tcaaatacgt tttatcttaa aagcgacatt gcagttattc acaaaaagcc tacgccagta
    10621 caaatagtta atgtcaacta tcctaagcgg agtaaagctg tgattaacga agcttatttt
    10681 cgtacacctt caacaactga ttacaacggc gtttatcaag gttattatat tgattttgaa
    10741 gcaaaggaaa ctaaaaacaa gacgtccttt cctttaaata atattcatga ccatcaagtc
    10801 gaacatatga aaaatgcata tcaacaaaaa ggtattgtgt ttttaatgat tcgttttaaa
    10861 acgctagatg aagtttatct tttaccctat tcaaaattcg aagtattttg gaagagatat
    10921 aaagataata ttaaaaagtc tataacagtt gatgaaatac gaaaaaatgg ttaccatatt
    10981 ccttatcagt atcaaccaag attagactat ctaaaagcag ttgataagtt gatattagat
    11041 gaaagtgagg accgcgtatg acggaaaaca aaggatcttc tcagcctaag aaaaacggta
    11101 ataatggtgg gaaatccaac tcaaaaaaga atagaaatgt gaagagaacg attattaaga
    11161 ttattggctt catgattatt gcatttttcg ttgttctttt actaggtatc ttattgtttg
    11221 cttattatgc ttggaaagca cctgctttta ccgaagctaa attacaagat ccgattcctg
    11281 caaagatata tgacaagaac ggagaacttg ttaaaacatt agataatggc caaagacatg
    11341 agcatgtaaa tttaaaagac gtgccgaaat caatgaaaga cgcagtactt gcaactgaag
    11401 acaatcgttt ctacgaacat ggcgcacttg attataaacg tttattcggt gcaattggta
    11461 agaacttgac tggtggattt ggttctgaag gtgcctcaac attaacacaa caagttgtta
    11521 aagatgcatt tttatcacaa cataaatcta ttggacgtaa agctcaagaa gcatacttat
    11581 catatcgttt agaacaagag tatagtaaag atgatatctt ccaagtatat ctaaacaaaa
    11641 tttactattc tgatggcgta acaggtatta aagctgctgc taagtattac tttaataaag
    11701 atttaaaaga tttaaactta gcggaagaag cttatttagc cggtttacct caggttccaa
    11761 acaactataa tatttatgat catccaaaag ctgctgaaga tcgtaaaaac actgttttat
    11821 acttaatgca ttatcataaa cgcattacag ataaacagtg ggaagatgct aagaaaatcg
    11881 atttaaaagc gaacttagta aatcgtactc ctgaagaacg tcaaaacatt gatacaaatc
    11941 aagattctga gtataattca tacgttaact ttgtaaaatc tgaattaatg aataataaag
    12001 cattcaaaga tgaaaattta ggtaatgtat tacaaagtgg tattaaaatt tatacaaaca
    12061 tggataaaga tgttcaaaaa acattacaaa atgatgttga taatggtagc ttctacaaga
    12121 ataaagacca acaagttggt gcaacaattc ttgatagtaa aactggtggt ttagttgcta
    12181 tatctggtgg acgtgatttc aaagacgtcg ttaacagaaa ccaagcaaca gatcctcacc
    12241 ctactggttc atctttaaaa cctttcttag cgtatggacc tgccattgaa aatatgaaat
    12301 gggcaacaaa ccatgcgatt caagatgaat cttcatatca agttgatggt tctacattta
    12361 gaaactatga tacgaagagt cacggtactg tatctattta tgatgcttta cgacaaagtt
    12421 tcaatatccc agctttaaaa gcttggcaat cagttaagca aaatgctggt aatgatgcac
    12481 ctaagaaatt cgctgccaaa cttggcttaa actacgaagg cgatattggt ccatctgaag
    12541 tacttggtgg ttctgcttca gaattctcac caacacaatt agcatcagca tttgctgcaa
    12601 tcgctaacgg tggtacttat aacaacgcgc attcaattca aaaagtagtt actcgtgatg
    12661 gtgaaacaat cgaatacgat catactagcc ataaagcgat gagtgattac actgcataca
    12721 tgttagctga gatgctaaaa ggtacattta aaccatatgg ttctgcatat ggccatggtg
    12781 tatctggagt aaatatgggt gctaagacag gtactggtac ttacggtgct gaaacttatt
    12841 cacaatataa tttacctgat aatgcagcga aagacgtgtg gattaacggc tttacacctc
    12901 aatacactat gtcagtgtgg atgggcttca gtaaagttaa acaatatggt gaaaactcat
    12961 ttgtgggaca tagccaacaa gaatatccac agttcttata tgaaaatgtg atgtcaaaaa
    13021 tttcatctag agatggcgaa gactttaaac gtcctagctc agtaagtggt agtatcccat
    13081 caatcaatgt ttctggtagt caagataaca acactacaaa tcgtagtaca cacggtggta
    13141 gtgacacatc agcaaacagc agtggtactg cacaatcaaa taacaatact agatctcaac
    13201 aatctagaaa cagcggtgga ttaacaggta tattcaacta atccactcaa cataaaatcc
    13261 tcagttatac catatttatg gtgtagccga ggattttttt aggttcttca tcttttatgg
    13321 tgggaaggta aaacttcctg ctttttttat aagtattcat acgctattgc tatattagta
    13381 gaaaccgtaa aatcagtcat tcacatacat tttgaaacat ctaaatagtt tttcatattc
    13441 acatctttag ctatgtaaaa ttattgaatt tgaataagtg gttgttccca tttttaaaat
    13501 tatgattata ttttctcata gtcatcttag acgctgtttt aaattgatat atttattaat
    13561 tccctgtgta cgttaatcag tgaaatatca acatgtttgc aacatatttt cacaatcata
    13621 gatataagaa atccacctac gattcgaaat agatatctta aagcatatct caagatatcc
    13681 attatgatgt agatggacag tttttatatt aagacgttgt aatgacacga cacctaacat
    13741 aattataaat atgtgtgcat gaattagttt tatatatttt caacattagg aaatgatcaa
    13801 tcttgtctat acctgcttat tctttaacct tgtttgataa ctagcattca ttttaatcaa
    13861 ttcagtttta aaggtcttca tttgtcgata gcccataaaa tgatatttac gttcaatcat
    13921 ataatcatat ctttctttaa aattcattgg cacaacagga tattgattta atttatcaaa
    13981 atcaatattg ccagttatgt cattaacctg cttaaaataa tcaataattt tactatggta
    14041 ttcatccaag tagggctttg cttcactaga acctaatgtc ttatttttgg caaataaatc
    14101 aatctcttgt tctaaattat caaatacctc ttttgtaata tttgtcatgt tatcacgctt
    14161 ctttcaaact agctttataa cgtttttgtc cttctctaca atcttctaat agtggacaaa
    14221 tatcgcattt aggttttcgg gctaaacagt ggtatcttcc aaagaaaatg agttgatgat
    14281 ggctcctatt ccatctatct ctaggtatga cagaacataa acggtcttct acctgtctca
    14341 cattatcttt ccaacgatta atacctaagc gtttagaaac acgttctaca tgcgtatcaa
    14401 cagctaatga aggttcatca aatgctacac tcatgactac attagcagtt ttacgtccta
    14461 cacctgctaa actttctaat tccttatgtg tttgtggtat ttctccatta aattgatcaa
    14521 tcaaagattg acaaagtttc ttaatattct tagctttgtt acgatacaga ccgatagaac
    14581 gaatatcatt cataagttct tcatcactga ctgccaaata atcttcaggc gttttgtatt
    14641 ttttaaacag ctcagttgtt actctattta ctagaacgtc tgtacattgc gctgacaata
    14701 atacagcaat agttaattcg aacggattat catgttttaa ttcacattct gcatccggaa
    14761 acatatttgc tataacatca atcatttcta atgctttttt cttacttacc atcaaggttc
    14821 tccccgttta accaatcaaa tttaggtacc gttttaactg tgtgtgtcat tttcggttta
    14881 ttgaattttt ctcttatttt tctagaatcg tcaattgttt tgacattgtt tttcttccaa
    14941 ttaagtaaaa tacgatctat atatttaaag ctaagtttat tcaaactatt cgcctcgtct
    15001 aatgccgctt gtataattgc agtatcgtgt ttatcaacat caatccattg atttaacgtt
    15061 tctatttcat atggagataa cggccttgca aatgtatcct ctaaaactct aaataattgt
    15121 ttaaattttt ctttactatt tagctctttc gtttccatac tttgttgctt caatatatga
    15181 cttaattttt cgaaaaaagg atctagattc atatattcgg taaatctacc ttcttcatct
    15241 ttttgaactt gtaattctag caattcacgt tgtatcaaat tttgaataac cattgtaata
    15301 tcacgtggtt gcatagttga gcccttctga agcaattcaa ttgaaggctg tttatttgat
    15361 gtttcggaag cataaatcaa tttaagcaaa atgactaaat cttgctcatc taaacctaag
    15421 tcactgtaat ggtctaataa ttctcttcgt atcactacag gtcttgcttt taattgatat
    15481 ttatccatgt tcgtaccccc ttttaaattt aaccattact taatagtata aatcattttc
    15541 tttaattttc gactaaaatg cttaatatat ttaactagta gcaaacaaat tttaaataac
    15601 agtatttttt aacactagat tttaacaatg aactttatgg ataatcagat gaactattta
    15661 ttttccaaat atagttacta aacaa
//
LOCUS       DAXWFK010000049        15635 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.049, whole genome shotgun sequence.
ACCESSION   DAXWFK010000049 DAXWFK010000000
VERSION     DAXWFK010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15635
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.049"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            118..1431
                     /gene="murT"
                     /locus_tag="ACGEXS_001201"
     CDS             118..1431
                     /gene="murT"
                     /locus_tag="ACGEXS_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II isoglutaminyl synthase subunit MurT"
                     /protein_id="HFF8012219.1"
                     /translation="MRQWTAIHLAKLARKASRAVGKRGTDLPGQIARKVDTDILRKLA
                     EQVDDIVFISGTNGKTTTSNLIGHTLKANNIQIIHNNEGANMAAGITSAFIMQSTPKT
                     KIAVIEIDEGSIPRVLKEVTPSMMVFTNFFRDQMDRFGEIDIMVNNIAETISNKGIKL
                     LLNADDPFVSRLKIASDTIVYYGMKAHAHEFEQSTMNESRYCPNCGRLLQYDYIHYNQ
                     IGHYHCQCGFKREQAKYEISSFDVAPFLYLNINDEKYDMKIAGDFNAYNALAAYTVLR
                     ELGLNEQTIKNGFETYTSDNGRMQYFKKERKEAMINLAKNPAGMNASLSVGEQLEGEK
                     VYVISLNDNAADGRDTSWIYDADFEKLSKQQIEAIIVTGTRAEELQLRLKLAEVEVPI
                     IVERDIYKATAKTMDYKGFTVAIPNYTSLAPMLEQLNRSFEGGQS"
     gene            1433..2164
                     /locus_tag="ACGEXS_001202"
     CDS             1433..2164
                     /locus_tag="ACGEXS_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFF8012220.1"
                     /translation="MHELTIYHFMSDKLNLYSDIGNIIALRQRAKKRNIKVNVVEINE
                     TEGITFDECDIFFIGGGSDREQALATKELSKIKTPLKEAIEDGMPGLTICGGYQFLGK
                     KYITPDGTELEGLGILDFYTESKTNRLTGDIVIESDTFGTIVGFENHGGRTYHDFGTL
                     GHVTFGYGNNDEDKKEGIHYKNLLGTYLHGPILPKNYEITDYLLEKACERKGIPFEPK
                     EIDNEAEIQAKQVLIDRANRQKKSR"
     gene            2250..2360
                     /locus_tag="ACGEXS_001203"
     CDS             2250..2360
                     /locus_tag="ACGEXS_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012221.1"
                     /translation="MDGNNQIDVCFFVLVNNYYTMSIYPRIINSNGDYAT"
     gene            2389..2802
                     /locus_tag="ACGEXS_001204"
     CDS             2389..2802
                     /locus_tag="ACGEXS_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012222.1"
                     /translation="MKPTKVILKDASYLHSKTSITFILKDVVIEEDNKIYYFDTSATF
                     EDQEVKFEFALFDSDMDNLKHMDCDNPVTEIYFIEPDLHFTIIDFNQELLCIYIDFDS
                     GLRHSNMATESGISLRINVAKSDFTKFINELASLH"
     gene            complement(3233..4219)
                     /locus_tag="ACGEXS_001205"
     CDS             complement(3233..4219)
                     /locus_tag="ACGEXS_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFF8012223.1"
                     /translation="MNDQWYKHLIGARTIKTGIAIFLTAVFCMALDLTPIYAILTAVV
                     TIEPTAKASLIKGYRRLPATVIGAGFAVLFTYLFGDQSPFTYALSATFTILFCTKLKL
                     QVGTNVAVLTSLAMIPGIHDAYIFNFLSRTLTAIIGLVTSGLINFMVFPPKYYGQVEE
                     KLSKTDALMYKLFYNRCQELILSRLQSDKSEKAYKNIFNLNNQVETLISYQRDELSYH
                     KKKECDWKLLNQLTKRAYTNRLFITHLSNIIYLPKNTRVNFSGDEKMALLKISSSIKD
                     IFYDGSFKREDDSVETLRSTIKALEISGENQIKSHILYEVLMIYRLLDSRYA"
     gene            4806..5564
                     /gene="map"
                     /locus_tag="ACGEXS_001206"
     CDS             4806..5564
                     /gene="map"
                     /locus_tag="ACGEXS_001206"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFF8012224.1"
                     /translation="MIVKTEEELQALKEIGYICAKVRNTMQAATKPGITTKELDNIAK
                     ELFEEYGAISAPIHDENFPGQTCISVNEEVAHGIPSKRVIREGDLVNIDVSALKNGYY
                     ADTGISFVVGESDDPMKQKVCDVATMAFENAIAKVKPGTKLSNIGKAVHNTARQNDLK
                     VIKNLTGHGVGLSLHEAPAHVLNYFDPKDKTLLTEGMVLAIEPFISSNASFVTEGKNE
                     WAFETSDKSFVAQIEHTVIVTKDGPILTTKIEEE"
     gene            5781..6167
                     /locus_tag="ACGEXS_001207"
     CDS             5781..6167
                     /locus_tag="ACGEXS_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012225.1"
                     /translation="MNYVERYIEQFLRATVRNNIKHYLLMLDEKMKNLDDYMRYLITK
                     KEQLSKLIDSLMLTLENKYIDIAEAFQIQCAREINNQEIENIKSELNKVEAYYAQIET
                     QIQQTSTEKIATEKTSYLINYMNAVA"
     gene            6182..6883
                     /gene="liaF"
                     /locus_tag="ACGEXS_001208"
     CDS             6182..6883
                     /gene="liaF"
                     /locus_tag="ACGEXS_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFF8012226.1"
                     /translation="MTHKYISTQMLIIFTALMIIANFYYIFFEKIGFLLVLLLGCVLV
                     YVGYLYFHKIRGLLAFWIGALLIAFTLLSNKYTIIILFVFLLLLIVRYLIHKFKPKKV
                     VATDEVMTSPSFIKQKWFGEQRTPVYVYKWEDVQIQHGIGDLHIDLTKAANIKENNTI
                     VVRHILGKVQVILPVNYNINLHVAAFYGSTYVNEKSYKVENNNIHIEEMMKPDNYTVN
                     IYVSTFIGDVEVIYR"
     gene            6880..7923
                     /locus_tag="ACGEXS_001209"
     CDS             6880..7923
                     /locus_tag="ACGEXS_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFF8012227.1"
                     /translation="MNHYIRTIGSMLILVYSMLAAFLFIDKVFVNIIYFQGMFYTQIF
                     GIPVFLFLNLIIILLCIIVGSVLAYKINQQNDWIKTQIERSMEGETVGINDQNIEIYS
                     ETLDLYHTLVPLNQELHKLRLKTQNLTNENYNINDVKVKKIIEDERQRLARELHDSVS
                     QQLFAASMMLSAIKETKLEPPLDQQIPILEKMVQDSQLEMRALLLHLRPLGLKDKSLG
                     EGIKDLVIDLQKKVPMKVVHEIQDFKVPKGIEDHLFRITQEAISNTLRHSNGTKVTVE
                     LFNKDDYLLLRIQDNGKGFNVDEKLEQSYGLKNMRERALEIGATFHIVSLPDSGTRIE
                     VKAPLNKEDSYDD"
     gene            7913..8542
                     /gene="vraR"
                     /locus_tag="ACGEXS_001210"
     CDS             7913..8542
                     /gene="vraR"
                     /locus_tag="ACGEXS_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator VraR"
                     /protein_id="HFF8012228.1"
                     /translation="MTIKVLFVDDHEMVRIGISSYLSTQSDIEVVGEGASGKEAIAKA
                     HELKPDLILMDLLMDDMDGVEATTQIKKDLPQIKVLMLTSFIEDKEVYRALDAGVDSY
                     ILKTTSAKDIADAVRKTSRGESVFEPEVLVKMRNRMKKRAELYEMLTEREMEILLLIA
                     KGYSNQEIASASHITIKTVKTHVSNILSKLEVQDRTQAVIYAFQHNLIQ"
     gene            complement(8658..9875)
                     /locus_tag="ACGEXS_001211"
     CDS             complement(8658..9875)
                     /locus_tag="ACGEXS_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFF8012229.1"
                     /translation="MSKKDHSSSKYLNSVKEAQEESKKKNKSNPKIDVDRTYIEPQQF
                     QSKKPKKDDQVFFLSRLNKPAKYKKDSNFLSYLIYRIGKDDASGLAAQMTYHFVLAMF
                     PMLLFLLTLLPFFNIKQSQITNMLSNAPAETSTLIKSVIGDITQNSSGGLLSIGLILA
                     IWSASNGMTAIMNSFNVAYDVEDSRNGIVLKLLSVVFTVVMGVVFVVALALPTFGSVI
                     SHFLFGPLGFDEQVKWIFNLIRIVLPIIIIFIIFIVLYSVAPNVKTKLKSVLPGAVFT
                     SIIWLAGSFGFGWYISNFGNYSKTYGSIAGIIILLLWLYITSFIIIVGAEINAIIHQR
                     SVIKGKTPEEAALEHDDNNKNHYNENTRYEYDEDSNATHQRTYHVDEHPSDTYPEDDK
                     NIKDKIVDKLKKD"
     gene            complement(10153..10428)
                     /locus_tag="ACGEXS_001212"
     CDS             complement(10153..10428)
                     /locus_tag="ACGEXS_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFF8012230.1"
                     /translation="MENKFVPGILIGAVIGGAISLADKSTRQALVQSVKDAKNGNRTR
                     KPSKVSKIKDEVLYWKDVVEEIRRNNPELERSLKDAKETFVNRKNQR"
     gene            complement(10435..10899)
                     /locus_tag="ACGEXS_001213"
     CDS             complement(10435..10899)
                     /locus_tag="ACGEXS_001213"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFF8012231.1"
                     /translation="MVDVAFVCLGNICRSPMAEAIMRQRLKDRNIHDIKVHSRGTGSW
                     NLGEPPHEGTQKILNKHNIPFDGMISELFEATDDFDYIVAMDQSNVDNIKSINPNLKG
                     QLFKLLEFSNMEESDVPDPYYTNNFEGVYDMVLSSCDNLIDYIVKDANLKEG"
     gene            11046..11246
                     /locus_tag="ACGEXS_001214"
     CDS             11046..11246
                     /locus_tag="ACGEXS_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1128 family protein"
                     /protein_id="HFF8012232.1"
                     /translation="MTNEEKVLAIREKLNIVNQGLLDPEKYKNANEEELTDIYDFVQS
                     RERLSPSEVTAIADALGQLRHE"
     gene            11249..12505
                     /locus_tag="ACGEXS_001215"
     CDS             11249..12505
                     /locus_tag="ACGEXS_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFF8012233.1"
                     /translation="MGILTNYKEKLQQYAELLVKVGMNVQPKQPVFIRSSVETLELTH
                     LIVEEAYHCGASDVRVVYSDPTLKRLKFENESVEHFANHEIKSYDVEARMDYVKRGAA
                     NLALISEDPDLMDGIDSQKLQAFQQQNARAFKGYMESVQKNQFPWVVAAFPSKAWAKR
                     VYPELSVEEAYIKFIDEVFDIVRIDGNDPVENWRQHIANLSVYAQKLQQKNYHALHYV
                     SEGTDLTVGLAKNHIWEDATSYVNGKEQAFIANIPTEEVFTAPDRNRVDGYVTNKLPL
                     SYNGTIIDQFKLMFKDGEIIDFSAGKGEAVLKDLINTDEGSRRLGEVALVPDDSPISN
                     RNTIFYNTLFDENAACHLAIGSAYAFNIQGGTEMTVEEKIASGLNDSNVHVDFMIGSS
                     DLTIYGIFEDGSKELVFENGNWASTF"
     gene            12595..13125
                     /locus_tag="ACGEXS_001216"
     CDS             12595..13125
                     /locus_tag="ACGEXS_001216"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFF8012234.1"
                     /translation="MTNQDRPMKSMSESKCYKNRQVFPQDTNHHHTMFGGTLMANIDE
                     IAAITAMKHAGAQVVTASTDSVDFLKPIKTGDILQYVAMVSYAGTSSMEVVVQIRIDD
                     VFNNKHDLAALSYLTFVALDDEGKPKHVPGVYPEDDVEKWFYDTAPQRVERRKARRIE
                     SKQTIEYLAQVQHIRD"
     gene            complement(13385..14536)
                     /gene="yfkAB"
                     /locus_tag="ACGEXS_001217"
     CDS             complement(13385..14536)
                     /gene="yfkAB"
                     /locus_tag="ACGEXS_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/CxCxxxxC motif protein YfkAB"
                     /protein_id="HFF8012235.1"
                     /translation="MNSVKLKQPVSIYNDPWEAYNDVKEHGQLTLSNIEFTTTNLCNM
                     RCSHCAVGYTLQTVDPEPLDMDLIYRRLDEIPNLRTMSITGGEPMFSKKSIRNVVKPL
                     LKYAHHRGIYTQMNSNLTLPQDRYLDIAEYIDVMHISHNWGTTDEFANVGFGAMKKQP
                     PLKAKLKLYEQMISNARTLSEQGMFVSAETMLNQSTLPHLRKIHQEVVHDMKCSRHEI
                     HPMYPADFASQLNVLTLAEMKKTIHDILDFRDEDIWMLFGTLPVFPCLKDDEDQKLLS
                     RLRNAKNVTTRNDPDGRSRLNVNVFTGNVIVTDFGDETGTISNIQKDKLTDVFDKWLS
                     SDLAKSLNCHCSEFSCLGPNVLVKNMYYPNMDFKDNERHMHKQPQIIQF"
     gene            14760..14921
                     /locus_tag="ACGEXS_001218"
     CDS             14760..14921
                     /locus_tag="ACGEXS_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SE1561 family protein"
                     /protein_id="HFF8012236.1"
                     /translation="MNEQQTIEQIKARLNKFIEDIDHVNPDEVRVEDIDEWIGLLDQL
                     EEKVKLVSK"
     gene            15052..15567
                     /locus_tag="ACGEXS_001219"
     CDS             15052..15567
                     /locus_tag="ACGEXS_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFF8012237.1"
                     /translation="MTKKVAIILANEFEDIEYSSPKEALENAGFNTVVIGDTANSEVV
                     GKHGEKVTVDVGIAEAKPEDYDALLIPGGFSPDHLRGDTEGRYGTFAKYFTKNDVPTF
                     AICHGPQILIDTDDLKGRTLTAVLNVRKDLSNAGAHVVDESVVVDNNIVTSRVPDDLD
                     DFNREIVKQLQ"
BASE COUNT         5556 a         2263 c         2789 g         5027 t
ORIGIN      
        1 atctaaatga gaatgattat aatttagaaa cactgtatca atttgtgatt gagaaacttt
       61 tttgaagttg ccttgtcatg ttataatgat gcgagtggaa atttgaggag gaaacacatg
      121 agacagtgga cggcaatcca tctagcgaaa ttggcgcgta aagcaagtag agcagtaggt
      181 aaaagaggaa cagatttacc tggacaaatc gctagaaaag tggatacaga tatattaaga
      241 aaattagcag agcaagttga tgatattgta tttatcagtg gaacaaatgg taaaacaacg
      301 acttcaaact taattggaca tactttaaaa gcaaataata ttcaaattat acacaataat
      361 gaaggtgcta atatggctgc aggtataact tctgcattca tcatgcaatc aacacctaag
      421 actaaaattg cggtaatcga aattgatgaa ggttcgattc cacgtgtgtt aaaagaagtt
      481 acaccttcaa tgatggtatt tactaatttc tttagagatc aaatggatcg cttcggtgaa
      541 attgatatta tggttaataa cattgcagag acaattagta ataaaggcat caaattattg
      601 ctaaatgctg atgatccatt tgtgagtcgt ttgaaaatcg caagtgatac gattgtgtac
      661 tatggtatga aagcacatgc ccatgaattt gaacaaagta cgatgaatga aagtagatat
      721 tgtccaaact gtggtcgctt attgcaatac gattatattc attataatca aattggtcat
      781 tatcactgtc agtgtggttt caaacgagag caagcaaaat atgaaatatc aagttttgat
      841 gtggcaccgt ttttatattt aaatatcaat gatgaaaaat atgatatgaa aattgcaggt
      901 gactttaacg cttataacgc gttagcagca tatactgttt taagagagct agggttaaat
      961 gaacaaacaa ttaaaaatgg ctttgaaacg tatacatcag acaatggtcg tatgcagtac
     1021 tttaaaaaag aacgaaaaga agcgatgatc aatttagcta aaaatcctgc aggaatgaat
     1081 gcaagtttat cagttggtga acaattagaa ggcgaaaaag tgtatgttat ttcgctaaat
     1141 gataacgctg cagatggtcg agatacttca tggatttatg atgcagattt tgaaaaatta
     1201 tctaagcaac aaattgaagc tatcatcgtg acaggtacac gagcagaaga acttcaattg
     1261 cgattgaagt tagcagaggt tgaagtacca attatagttg agcgtgatat ttataaagca
     1321 acggcaaaga ctatggatta taaaggtttc acagttgcaa taccaaacta tacatcatta
     1381 gcgcctatgc ttgaacaatt aaaccgttcg tttgaaggag gtcaatcata atatgcatga
     1441 attgactatt tatcatttta tgtcagataa attgaattta tacagtgata taggaaatat
     1501 tattgcttta agacaacgtg ctaaaaaacg aaatattaaa gttaatgtcg tagaaatcaa
     1561 tgaaacagaa ggtattacct ttgatgaatg tgatattttc tttatcggtg gtggaagtga
     1621 tagagaacaa gcattagcaa caaaagaatt aagtaaaatt aagacaccac ttaaagaagc
     1681 gattgaagat ggtatgccgg gattaacgat ttgtggaggc tatcaatttt tagggaaaaa
     1741 atatatcacg cctgatggta cagaattaga agggttaggt attttagatt tttatactga
     1801 atcaaagaca aaccgattaa caggagatat tgttatcgaa agtgatactt ttggaactat
     1861 tgtaggtttt gaaaatcacg gtggtagaac atatcatgat ttcggtacac ttggtcatgt
     1921 tacttttggt tatggtaata atgatgaaga taaaaaagaa ggcattcatt ataaaaattt
     1981 attaggtact tatttacatg gaccaatttt acctaaaaat tacgaaatca ctgattatct
     2041 gttagaaaaa gcttgtgaac gtaagggtat tccgtttgag cctaaagaaa tagataatga
     2101 agcggaaata caagcgaaac aagtattaat agacagagca aatagacaga agaaatctcg
     2161 ttaactctga acatcgcatc aatggattta atattgataa acgatgaagt ttagtaatta
     2221 atcatatatg tataaacaca cacattattt tggatggaaa caaccaaatt gatgtgtgtt
     2281 tttttgttct agtgaataat tattatacaa tgagtatcta tcctagaatt atcaatagta
     2341 atggtgatta tgcaacatga aaaaatgaat gatgaaagga atttgacgat gaagcctact
     2401 aaagtgatat taaaagatgc atcttattta catagcaaaa catcgataac atttatttta
     2461 aaagatgtag ttatcgaaga agataataaa atttattatt tcgacactag tgcaactttc
     2521 gaagatcaag aagttaaatt tgaatttgca ctctttgata gtgacatgga caatttgaaa
     2581 catatggatt gcgacaatcc tgttactgaa atatatttta tagaacctga tttacatttt
     2641 acaattattg attttaatca agaactgctt tgtatttata ttgattttga ttctggttta
     2701 aggcattcaa acatggcaac agaatctggt atttcattaa ggataaatgt tgctaaatca
     2761 gattttacta aatttattaa tgaattagcc tctttacatt aatgatttaa atctgatatg
     2821 taattacaat caaaaaagac agccacatcc ctccgtagtt taggcgtgtg gctatatttg
     2881 agtatgaata tttatgcttg taattttaaa aagggacatg ctatatacga taaaaagagg
     2941 cggggacata aatcaatgtt ctatgctcta cgaagttata ttggcagtag ttgactgaac
     3001 gaaaatgcgc ttgtaacaag cttttttcaa ttctagtcag gggccccaac aaagagaaat
     3061 tggattccca atttctacag acaatgcaag ttggggtgtg ggccccaaca cagagaattt
     3121 cgaaaagaaa ttctacaggc aaagcgagtt ggggtgggac gacgaaataa attttatgaa
     3181 aatatcattt ctgtcccact cccatggtgc caattagcat aaggtactta aattaagcat
     3241 atctgctgtc tagcagtcga taaatcatta gaacttcgta tagtatatga cttttaattt
     3301 gattttcacc actaatttca agtgctttta tagtcgaacg taaagtttct acagaatcat
     3361 cttctctctt aaaagaacca tcataaaata tatctttgat gctactacta atttttagca
     3421 atgccatttt ttcgtcacct gaaaagttaa cacgagtatt tttaggcaag taaatgatat
     3481 ttgataaatg agtgataaac aaacgattcg tatatgcacg tttagttaat tgattgagta
     3541 atttccaatc acattctttt ttcttatgat agcttaattc atcacgttga taacttatta
     3601 acgtttcaac ttgattattt aaattgaaaa tatttttata tgctttttcg cttttatcag
     3661 attgcagtct tgataagata agttcttggc agcgattgta aaataattta tacatcaagg
     3721 catctgtctt acttaatttt tcttcgacct gaccataata cttaggtgga aacaccatga
     3781 agttaattaa acctgatgtc acgagtccaa taattgctgt caatgttcga gacaaaaagt
     3841 tgaatatgta ggcatcatga atacctggaa tcatagctaa tgatgttagt acagcgacat
     3901 tcgtaccaac ttgcaatttg agttttgtac agaataaaat cgtgaacgtt gcactcaatg
     3961 catatgtaaa aggtgattga tcgccgaata aatatgtaaa taatactgca aagcctgcac
     4021 caattaccgt agcaggtaat ctacgataac ctttaataag tgatgccttg gcagttggtt
     4081 caattgtgac tacagctgtt aaaatggcat agatgggtgt taaatctagt gccatacaaa
     4141 agacagctgt taaaaaaatg gcaataccag ttttaattgt tctggcacca attaaatgtt
     4201 tataccattg atcgttcatt ttttaacctc tgatcatcgt aaaatcttag cgagcgcttt
     4261 ataataatag tatcgtacat tggaaaagtt catgtatgta aaatatttga aataatcata
     4321 cataagcatt actttgattt tcatatacat taatcaaagt tctgggctcc acaatgttat
     4381 attgttagta gttgactgaa ataaaatgca cttgtaacaa gcatttttca attatagtcc
     4441 ggggccccaa catagagaat ttcaaaaaag aaattctaca ggcaatgcag gttggcgggg
     4501 ccccaacaca gaagctgacg aaaagtcagc ttacaataat gtgcaagttg gggtgtgggc
     4561 cccaacacag aagctgacga aaagtcagct tacgataatg tgcaggttgg cggggcccca
     4621 acatagagaa attggatcta caatttctac aggcaatgca agttggggta caacgataaa
     4681 gaaatatttt ttctttatca cactatgtct cactcacttt ccaaaatact aaagtaacat
     4741 ctttagtata tcaaagaatt tttgctataa taagttataa ttatataaaa aaggaacggg
     4801 ataaaatgat tgtaaaaaca gaagaagaat tacaagcgtt aaaagaaatt ggatacatat
     4861 gcgctaaagt gcgcaataca atgcaagctg caaccaaacc aggtatcact acgaaagagc
     4921 ttgataatat tgcgaaagag ttatttgaag aatacggtgc tatttctgcg ccaattcatg
     4981 atgaaaattt tcctggtcaa acgtgtatta gtgtcaatga agaggtggca catgggattc
     5041 caagtaagcg tgtcattcgt gaaggagatt tagtaaatat tgatgtatcg gctttgaaga
     5101 atggctatta tgcagataca ggcatttcat ttgtcgttgg agaatcagat gatccaatga
     5161 aacaaaaagt atgtgacgta gcaacgatgg catttgagaa tgcaattgca aaagtaaaac
     5221 cgggtactaa gttaagtaac attggtaaag cggtgcataa tacagctaga caaaatgatt
     5281 tgaaagtcat taaaaactta acaggtcatg gtgttggttt atcattacat gaagcaccag
     5341 cacatgtact taattacttt gatccaaaag acaaaacatt attaactgaa ggtatggtat
     5401 tagctattga accgtttatc tcatcaaatg catcatttgt tacagaaggt aaaaatgaat
     5461 gggcttttga aacgagcgat aaaagttttg ttgctcaaat tgagcatacg gttatcgtga
     5521 ctaaggatgg tccgatttta acgacaaaga ttgaagaaga atagttcaac atatactaag
     5581 actaaagtat gaacatcatt tagttccgga gcctattcat attggtttcg gaactgtttt
     5641 ataataatta agaacacaat caatgcgtca tttcaaaaat atgttgtaac aaagtagttt
     5701 ttaagcaaac atatcatcga catcaacgaa gatacatagc gcatttggta ttttaaaact
     5761 tattataaaa ggtgatagtt atgaactatg ttgaacgtta tattgaacag tttttgagag
     5821 caacagtaag aaataatatc aagcactacc ttttaatgct agatgaaaaa atgaaaaatt
     5881 tagatgatta tatgcgttat ttaattacta aaaaagaaca acttagcaag ttaattgaca
     5941 gtctaatgct aacattagaa aataaatata ttgatattgc tgaagcattt caaattcaat
     6001 gtgcaagaga aatcaataat caagaaattg aaaatattaa atcagagttg aataaagttg
     6061 aagcatatta tgcacaaatt gaaactcaaa ttcaacaaac ttcaactgaa aaaatagcaa
     6121 cagaaaaaac atcgtatcta ataaattata tgaacgctgt ggcatagaaa ggcggcgaaa
     6181 catgacacac aaatatatat caacgcaaat gttgatcatt tttactgcat taatgattat
     6241 tgccaatttt tactacatat tttttgaaaa aattggcttt ttactcgttc tattattggg
     6301 atgtgtatta gtttatgtag gatatcttta ttttcataaa atacgtggcc ttttggcgtt
     6361 ttggataggc gcgctattaa ttgcattcac attattgtct aataagtata caatcatcat
     6421 cttgttcgtc tttttattat tacttattgt gcgttattta atacacaagt ttaaaccaaa
     6481 aaaagtagtt gcgacggatg aggttatgac ttcaccatct tttattaaac aaaagtggtt
     6541 tggtgagcaa cgtacaccag tttatgtata taagtgggaa gatgtacaaa ttcaacatgg
     6601 aattggcgac ctacatattg acttaacaaa agctgcaaat attaaggaaa ataataccat
     6661 tgttgttaga cacattttag gtaaagtgca ggttatattg ccggttaatt acaatattaa
     6721 tttacatgta gctgcttttt atggaagtac ttacgtgaat gaaaaatcat ataaagttga
     6781 aaataacaat attcatattg aagaaatgat gaaaccggat aactatacag ttaatatcta
     6841 cgtatcaacg tttatcggag acgtagaggt gatttatcga tgaaccacta cattagaaca
     6901 attggttcaa tgctcatctt agtatatagc atgctagctg catttctgtt catcgataaa
     6961 gtttttgtaa atatcatcta ttttcaaggt atgttttata cacaaatatt cggaatacca
     7021 gtttttttat ttttaaatct catcatcata ttattgtgta ttattgttgg ttcggtactc
     7081 gcttacaaaa tcaatcagca aaatgattgg attaagacgc aaattgagcg ttcaatggaa
     7141 ggcgaaacag ttggcattaa tgatcaaaat atagaaatat atagtgaaac gttagattta
     7201 taccatacac tcgtaccttt aaatcaagaa ttgcataagt tgcgacttaa aactcaaaac
     7261 ttaaccaatg aaaattataa tattaatgat gtgaaagtta aaaagattat tgaagatgaa
     7321 cgtcaaagac tagcacgaga acttcacgat tctgttagtc agcaactttt tgcggcaagt
     7381 atgatgctat ctgctatcaa agaaacgaag ttagaaccac cattagacca acaaattcct
     7441 attttagaga aaatggttca agattcgcag ttagaaatgc gtgctttgct gttacattta
     7501 agaccgcttg gtttaaaaga caaatcttta ggtgagggta ttaaagattt agttattgat
     7561 ttacaaaaaa aagtgccaat gaaagttgtg catgaaatac aagattttaa agtgcctaaa
     7621 ggtattgaag atcatttgtt cagaattaca caggaagcaa tttcgaatac attgcgtcat
     7681 tcaaacggta caaaagtgac agtagaattg tttaataaag acgattattt attgttgaga
     7741 attcaagata atggtaaagg ttttaatgtt gatgaaaaat tagaacaaag ttatggactt
     7801 aaaaatatgc gtgaaagagc tttggaaatt ggtgcaacgt tccatattgt atcattgcca
     7861 gattcaggta cacgtatcga ggtgaaagca cctttaaata aggaggattc gtatgacgat
     7921 taaagtattg tttgtggatg atcatgaaat ggtacgtata ggaatttcaa gttatctatc
     7981 aacgcaaagt gatattgaag tagttggtga aggcgcttct ggtaaagaag caattgccaa
     8041 agcccatgag ttgaagccag atttaatttt aatggattta cttatggatg acatggatgg
     8101 tgtagaagcg acgactcaga ttaaaaaaga tttaccgcaa attaaagtat taatgttaac
     8161 tagttttatt gaagataaag aggtatatcg tgcattagat gcaggtgtcg atagttacat
     8221 tttaaaaaca acaagtgcaa aagatatcgc cgatgcagtt cgtaaaactt ctagaggaga
     8281 atctgttttt gaaccggaag ttttagtgaa aatgcgtaac cgtatgaaaa agcgcgcaga
     8341 gttatatgaa atgcttacag aacgagaaat ggaaatatta ttattgattg cgaaaggtta
     8401 ctcaaatcaa gaaattgcta gtgcatcgca tattactatt aaaacggtta agacacatgt
     8461 gagtaacatt ttaagtaagt tagaagtgca agatagaaca caagctgtta tctatgcatt
     8521 ccaacataat ttaattcaat agttcatatc gaattaagaa aagttactta cgccaatcac
     8581 aatataacat caaatagaca ccaaaaaaca gaggttttcg caataatcat tagcgaaaac
     8641 ctctgttttg ttggtgttta gtctttttta agcttatcaa cgattttgtc ttttatattt
     8701 ttatcatctt ctggataggt atcactagga tgttcatcaa catgatacgt acgttgatgt
     8761 gttgcattac tatcttcatc atattcgtat ctagtgttct cattataatg atttttatta
     8821 ttgtcatcat gttctaatgc tgcttcttca ggtgttttac ctttaattac actacgctga
     8881 tgaatgattg cattgatttc agcaccgaca attataataa aacttgtgat atataaccat
     8941 agtaacaaaa tgatgatacc cgcgatactg ccatatgttt tagaatagtt accaaaattt
     9001 gaaatatacc aaccaaaacc aaatgaacca gctaaccaaa taattgaagt aaatactgca
     9061 cctggtaata ctgacttaag cttcgtttta acgttaggtg caaccgaata taacacgata
     9121 aatatgataa atataataat gattggtaac acaattctaa taaggttaaa aatccatttc
     9181 acttgttcgt caaatccaag tggaccgaat aggaaatgac taattacaga accaaacgtt
     9241 ggtaatgcta gagcaactac aaacacaacg cccataacta cagtgaagac aacacttagt
     9301 agttttaata cgattccatt acggctatct tctacatcgt aagcaacatt gaaagaattc
     9361 ataattgcag tcattccatt tgaagctgac caaattgcta aaatcaaacc gatagataat
     9421 aagccaccac tggagttttg agttatatca ccaattacac tcttaattag agtagatgtt
     9481 tcagcgggtg cattgcttaa catattagta atttgactct gcttaatatt gaaaaatggt
     9541 aataatgtta ataggaaaag caacataggg aacatagcaa gtacgaaatg gtaagtcatt
     9601 tgtgctgcta gtcctgaggc atcatctttt cctatgcgat agatgagata tgataagaaa
     9661 ttagagtctt tcttatattt tgcaggttta tttaatcttg ataagaagaa aacctgatca
     9721 tcttttttag gtttcttaga ttggaattgt tgaggttcaa tatatgtacg atcaacatca
     9781 attttgggat tacttttatt tttctttttt gactcctctt gcgcttcctt aacagaatta
     9841 aggtattttg aagaagagtg atcctttttc gacatgtgca ctccttaacc tttctctatg
     9901 taatgtttta taaagtaact attagtattt atccaattat aaaaaaacta aacaatgata
     9961 tgctttaaaa atgataatat tatagaaatt aattgattta aatctttcat aggctatttt
    10021 gatttaaaat taaaacaaag gcaatactga aacaatgcag tttggccaat cattgtatta
    10081 tcatttaata atgataacta tgaaaggcgt acaccaaatt gttaagtatt gccttataaa
    10141 aacaaaatca tcttagcgtt gattttttct attaacaaat gtttctttcg catcctttaa
    10201 tgaacgttct aattcaggat tattacgacg aatttcttca acaacatctt tccagtataa
    10261 aacttcgtct ttaatcttgc tgactttaga aggcttacga gtgcggttac cattttttgc
    10321 atctttaact gattgaacta aagcttgacg tgtagattta tcagctaaac taattgcacc
    10381 accaattacg gcaccaatta aaataccagg aacaaattta ttttccataa aaaactaccc
    10441 ctctttcaaa tttgcatctt ttacgatgta gtctattaaa ttatcacaag atgataatac
    10501 catgtcgtat acaccttcaa aattattcgt gtagtatgga tctggtacat cactctcttc
    10561 catattacta aattctaaca gtttgaacaa ttgtccctta agattaggat tgatagattt
    10621 aatattatca acgttacttt gatccatagc cacaatgtaa tcaaaatcat ctgtcgcttc
    10681 gaataattca ctaatcatgc catcaaatgg aatattgtgt ttgttgagaa ttttttgtgt
    10741 accttcatga ggtggctctc ctaaattcca gctaccagta cctcttgaat gtactttaat
    10801 atcatgaata tttctgtctt taagtctttg tcgcatgatt gcttctgcca ttggagaacg
    10861 acatatattg ccaagacaga caaatgctac atctaccatt ttgattcctc caaactatgt
    10921 agttatatcc ccattttata gcgactttaa acaataagaa agcagattat ataaaattct
    10981 attaaagttt attaaattgt gatactttga taacataact attattagag gtgaacattg
    11041 tggctatgac aaatgaagag aaagtattag ctattagaga gaagttaaat attgttaatc
    11101 aaggattatt agatcctgaa aaatataaaa atgcaaatga agaagaatta acagatatat
    11161 atgattttgt tcaatcaaga gaaagattgt cgccaagtga agtgacagct attgctgacg
    11221 ctttaggaca attgcgacac gaataggagt gggaattttg actaattaca aagaaaagtt
    11281 acaacaatac gctgaactat tagttaaggt tggtatgaat gttcaaccaa aacagccagt
    11341 atttattaga tcatcagtag aaacattaga attaacgcat ttaattgtag aagaagcata
    11401 tcattgtggc gcttcagatg tacgtgttgt atatagcgat ccaaccttaa aacgcttaaa
    11461 atttgaaaat gaatctgtgg aacattttgc taatcatgag ataaaatcat atgatgttga
    11521 agcgagaatg gattacgtta aacgtggcgc tgctaattta gcacttatta gtgaagatcc
    11581 tgatttaatg gatggtatcg atagccaaaa attacaagca tttcaacaac aaaacgcacg
    11641 cgcatttaaa ggatatatgg aaagtgtaca aaaaaatcaa tttccatggg tcgtagctgc
    11701 ttttccatct aaagcttggg cgaagagagt gtatcctgaa ctatctgtag aagaagcata
    11761 tattaagttt attgacgagg tattcgatat tgtgcgaatt gatggtaacg atccagttga
    11821 aaattggcgt caacacatcg ctaatttaag tgtttacgct caaaagttac aacaaaaaaa
    11881 ttaccatgcg ttgcattatg tatctgaagg tactgattta acagtaggtt tagcgaaaaa
    11941 tcatatctgg gaagatgcta caagttatgt taacggcaag gaacaagctt ttattgctaa
    12001 tataccaact gaagaagtat ttacagcacc agatcgaaat agagtagatg gatatgttac
    12061 taataaattg cctttaagtt ataatggcac aattatcgat caatttaaat tgatgtttaa
    12121 agatggagag attattgatt tttcagctgg aaaaggtgaa gcggtattga aagatttaat
    12181 caatactgat gaaggttcaa gaagattagg tgaagtagca ttagtacctg atgattcacc
    12241 gatatcaaat cgtaatacca ttttttacaa tactttattt gatgaaaatg cagcatgtca
    12301 tttagcgatt ggatctgctt acgcctttaa tattcaaggt ggaacggaaa tgactgttga
    12361 ggaaaagatt gcaagcggat taaatgattc aaatgtacat gtcgatttca tgattggtag
    12421 tagtgatttg actatttatg gcatattcga agatggttca aaagaactag tatttgaaaa
    12481 tggaaattgg gcatcaacat tttaataaat gttattttga ggtgctgagt aggaaatgaa
    12541 acatgtattt aaaggtcaaa tacgtgatta aagtatagat tgggagataa aataatgaca
    12601 aatcaggaca gaccaatgaa atctatgtca gaatcaaaat gttataaaaa tagacaagtt
    12661 ttccctcaag atacgaatca ccatcataca atgtttggtg gtacattgat ggctaatatt
    12721 gatgaaattg cagcaatcac agctatgaaa catgctggtg cgcaagtagt taccgcatct
    12781 acagactcag tagatttctt aaagccgatt aaaacagggg acatattaca atacgtagcg
    12841 atggtttcat acgctgggac tagttcaatg gaagtggtcg ttcaaattag aattgacgat
    12901 gtatttaata acaagcatga tttagctgca ttaagttact taacatttgt tgcgttagat
    12961 gatgaaggca aacctaaaca tgtacctggt gtatatccag aagatgatgt tgaaaaatgg
    13021 ttttatgata cagcaccaca acgtgttgaa cgaagaaaag cacgtcgcat tgaaagtaaa
    13081 caaacaattg aatatttagc tcaagtgcaa catattagag actaaaaata taaagacaga
    13141 ttgttaagga tgaattgaac tgttatgttc gaatattcca cctacaatct gtctttttga
    13201 ttatgcacgt ttatgctttt agttaaaatt tacccctttt attatagact attagtccta
    13261 ttaaatgccc tttattattt aagtagagta ctgccagcaa ttaattgttt tttattttat
    13321 aatagcatta aaatatgcgg agactactta tcgataaagt gcttctccgc ataattaaga
    13381 gtttttaaaa ttgtataatt tgtggttgtt tgtgcatatg acgctcatta tctttaaaat
    13441 ccatattcgg atagtacata tttttaacaa gaacatttgg tcctaaacaa ctaaactcgg
    13501 aacaatgaca atttaatgat ttagcaagat cagaggataa ccatttatca aatacatctg
    13561 ttaatttatc tttttgtata ttcgaaattg tacctgtttc atctccgaaa tcagttacga
    13621 ttacattacc tgtaaataca ttgacattta aacgactacg gccatccggg tcatttctag
    13681 tcgttacatt tttagcattt cttaaacgtg atagtaactt ttgatcttca tcatccttta
    13741 agcatggaaa cacaggcaaa gtaccaaata acatccaaat atcttcatct ctgaaatcca
    13801 atatatcatg aattgtcttt ttcatttccg ctagagttaa cacatttaat tgacttgcaa
    13861 agtcagctgg atacataggg tgaatctcgt gtctgctaca tttcatatca tgaacgactt
    13921 cttgatgtat ttttcgtaaa tgtggtagcg tactttgatt gagcattgtt tccgcagata
    13981 caaacattcc ttgttctgat aatgtacgtg cattcgaaat catttgttca tataatttta
    14041 acttagcttt taacggtggt tgcttcttca ttgcgccaaa gccaacattt gcgaattcat
    14101 cagttgttcc ccagttgtgt gagatatgca taacatcgat atattcagca atatctaaat
    14161 aacgatcttg aggcaatgtt aggtttgaat tcatttgtgt atatatacct cgatgatgtg
    14221 catactttaa tagaggttta acaacatttc taatagactt tttagaaaac attggttcgc
    14281 cacctgtaat agacatcgtt cgcagatttg gaatttcatc aagtctacga taaattaagt
    14341 ccatatctaa aggctcgggg tcgacagttt gtaaagtata accaactgca cagtggctac
    14401 aacgcatatt acaaagattt gtagttgtaa attcgatgtt acttaaagtt aattggccat
    14461 gttctttaac atcgttatat gcttcccatg gatcattgta aatgctaaca ggttgcttca
    14521 atttgacgct attcatcgtc ttaaacttcc ttctttctat ttggttcata tcggtatgat
    14581 accacttcgt attatcactc gtaacaaatt atctcttaat tttagttaat tgaaaagggg
    14641 atttttaaaa gtcaaaataa tttgaatgtc ataaatatag ctaagattat atcgctttaa
    14701 cgcaatttct gttaggataa taatgaaaaa gaaaacaaaa taaaaggagt taacttttta
    14761 tgaatgaaca gcaaacaatc gaacagataa aagcgcgttt aaataagttt attgaggata
    14821 tcgatcatgt aaatcctgat gaagtacgtg ttgaagatat agatgaatgg attggattgt
    14881 tagatcagct tgaagaaaag gttaaattag tatctaagta aatgccacat aagatgtgtt
    14941 agggttagat atcgcgacag tcgcttaata ttttttatat cttgtatgtt taatcactga
    15001 tttacttgtc tatagtaatt atatcaatga tatatagaaa ggtgattttc aatgactaaa
    15061 aaagtagcaa ttattctagc aaacgaattt gaagatatag aatattcaag ccctaaagag
    15121 gcattagaga atgcaggctt taatactgta gtgattggag atactgcaaa tagtgaagtt
    15181 gttggtaaac acggtgaaaa agttactgtc gatgtaggca ttgcagaagc taaaccagaa
    15241 gattatgatg cattattaat tcctggagga ttttcaccag atcatttacg tggagataca
    15301 gaaggtcgat atggcacatt tgctaaatac tttactaaaa atgatgtacc aacatttgcc
    15361 atttgtcatg ggccacaaat actaatagat acagacgatt taaaaggtcg tacgttaaca
    15421 gcagtattaa atgtacgcaa agatttatca aatgcaggcg cacatgtagt tgatgagtca
    15481 gtagttgtag acaacaatat tgtaacaagt cgagtaccag acgatttaga tgattttaat
    15541 cgagaaatcg ttaaacaatt acaatagaag tattgtggtt atcgattgtt aaaatttata
    15601 tattttcgtt tgaaatgaaa tatggatttt agttt
//
LOCUS       DAXWFK010000050        15322 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.050, whole genome shotgun sequence.
ACCESSION   DAXWFK010000050 DAXWFK010000000
VERSION     DAXWFK010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15322
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.050"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(396..842)
                     /gene="perR"
                     /locus_tag="ACGEXS_001220"
     CDS             complement(396..842)
                     /gene="perR"
                     /locus_tag="ACGEXS_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HFF8012238.1"
                     /translation="MSVEIESIEHELEESIASLRQAGVRITPQRQAILRYLISSHTHP
                     TADEIYQALSPDFPNISVATIYNNLRVFKDIGIVKELTYGDSSSRFDFNTHNHYHIIC
                     EQCGKIVDFQYPQLNEIERLAQHMTDFDVTHHRMEIYGVCKECQDK"
     gene            complement(939..1889)
                     /locus_tag="ACGEXS_001221"
     CDS             complement(939..1889)
                     /locus_tag="ACGEXS_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFF8012239.1"
                     /translation="MKVVGLNRMREVETELQQRFSDLDFKFYKKASEIPESDLADLDI
                     LVGYDGGINEAFLRRCPNLKWIAWFATGVNTLPLDYIADHGILLTNGKGVQAKQLSEY
                     ILAFILDDYKKMKLSYDNQRQHIYDSKITGKRLSGQTVLFLGTGAIATRTAKLAKAFN
                     MNLIGLSKSGQNKDEFDEIYTIESLESTLPNADIIINALPETQETIHLLKKKHFELMK
                     DEALFINIGRGSIVKEALLIEVLKSKVIRHAYLDVFENEPLKPNHELYELDNVTITAH
                     ITGNDYEAKYDLLDIFKNNLVNFLNKNGLIENEVDAKKGY"
     gene            complement(1895..2350)
                     /gene="bcp"
                     /locus_tag="ACGEXS_001222"
     CDS             complement(1895..2350)
                     /gene="bcp"
                     /locus_tag="ACGEXS_001222"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFF8012240.1"
                     /translation="MLQKGEQFPIFKLENQDGTVITNDTLKGKKAIIYFYPRDNTPTC
                     TTEACDFRDNLEMFNDLDVAVYGISGDSKKKHQNFIEKHGLNFDLLVDEDFKLAKETG
                     VYQLKKSFGKESMGIVRTTFIIDEQGKVLDVIEKVKVKTQIEELKNILG"
     gene            2431..3720
                     /locus_tag="ACGEXS_001223"
     CDS             2431..3720
                     /locus_tag="ACGEXS_001223"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFF8012241.1"
                     /translation="MNFSESERLQQLSNEYILGGVNSPSRSYKAVGGGAPVVMKEGHG
                     AYLYDVDGNKFIDYLQAYGPIITGHAHPHITKAIQEQAAKGVLFGTPTELEIEFSKKL
                     RDAIPSLEKIRFVNSGTEAVMTTIRVARAYTKRNKIIKFAGSYHGHSDLVLVAAGSGP
                     SQLGSPDSAGVPESVAREVITVPFNDINAYKEAIEFWGDEIAAVLVEPIVGNFGMVMP
                     QPGFLEEVNEISHNNGTLVIYDEVITAFRFHYGAAQDLLGVIPDLTAFGKIVGGGLPI
                     GGYGGRQDIMEQVAPLGPAYQAGTMAGNPLSMKAGIALLEVLEQDGVYEKLDSLGQQL
                     EEGLLKLIEKHNITATINRIYGSLTLYFTDEKVTHYDQVEHSDGEAFGKFFKLMLNQG
                     INLAPSKFEAWFLTTEHTEEDIKQTLKAADYAFSQMK"
     gene            4013..5107
                     /locus_tag="ACGEXS_001224"
     CDS             4013..5107
                     /locus_tag="ACGEXS_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFF8012242.1"
                     /translation="MRLGARIFKTGIAIILAMSIASLLPDDVGLKALAGVSAVVAMQP
                     SIYRSFKTVSDQALGNIIGAILSVTMVTIFSDNFIIMGVTVIVLIAILFKFNLAHVAT
                     LASVTALIIMGQHTGSFYITAFYRFVLVMIGVISSSLVNFVFLPPKFETKIYYNSLNI
                     SSDIFMWFKLVLNDTTEFNNIKQDSHNLKQRVEKLEKIYDYYSEERPITKKHIHQQNR
                     KKILFREVVQTTRQAYEVLNKLSRYQNDLYLLNNNFLLQIKLDLDSLTAFHEQILASL
                     SKKARYNVTHVDYELDNPQKKDLLSTFQHELINHPYQTEYSFANVMQIVAAIEEYRHH
                     LEHLDRIRISFFTYHRSDADIEIVEEDFDL"
     gene            complement(5197..5289)
                     /locus_tag="ACGEXS_001225"
     CDS             complement(5197..5289)
                     /locus_tag="ACGEXS_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012243.1"
                     /translation="MFDFSTILKRKFVTGWWGHNIEKAVRDFLN"
     gene            complement(5298..7034)
                     /locus_tag="ACGEXS_001226"
     CDS             complement(5298..7034)
                     /locus_tag="ACGEXS_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAV1866 family putative multidrug efflux ABC
                     transporter"
                     /protein_id="HFF8012244.1"
                     /translation="MIKRYLQFVKPYKYRIFATIIVGIIKFGIPMLIPLLIKYAIDGV
                     INNHALTTDEKVHHLTIAIGIALFIFVIVRPPIEFIRQYLAQWTSNKILYDIRKKLYN
                     HLQALSARFYANNQVGQVISRVINDVEQTKDFILTGLMNIWLDCITIIIALSIMFFLD
                     VKLTLAALFIFPFYILTVYVFFGRLRKLTRERSQALAEVQGFLHERVQGISVVKSFAI
                     EDNEAKNFDKKNTNFLTRALKHTRWNAYSFAAINTVTDIGPIIVIGVGAYLAISGSIT
                     VGTLAAFVGYLELLFGPLRRLVASFTTLTQSFASMDRVFQLIDEDYDIKNGVGAQPIE
                     IKQGRIDIDHVSFQYNDNEAPILKDINLSIEKGETVAFVGMSGGGKSTLINLIPRFYD
                     VTSGQILIDGHNIKDFLTGSLRNQIGLVQQDNILFSDTVKENILLGRPTATDEEVVEA
                     AKMANAHDFIMNLPQGYDTEVGERGVKLSGGQKQRLSIARIFLNNPPILILDEATSAL
                     DLESESIIQEALDVLSKDRTTLIVAHRLSTITHADKIVVIENGHIVETGTHRELIAKQ
                     GAYEHLYSIQNL"
     gene            complement(7325..7867)
                     /locus_tag="ACGEXS_001227"
     CDS             complement(7325..7867)
                     /locus_tag="ACGEXS_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFF8012245.1"
                     /translation="MVRESIPKEGENIKIQSYKHDGKIHRVWSETTILKGTDHVVIGG
                     NDHTLVTESDGRTWITREPAIVYFHSEYWFNVICMFREDGIYYYCNLSSPFVCDEEAL
                     KYIDYDLDIKVYPNGKYHLLDEDEYEQHMNQMNYPHDIDIILRRNVDILQQWIEQKKG
                     PFAPDFIKVWKERYKKIRQY"
     gene            complement(8169..9206)
                     /gene="mutY"
                     /locus_tag="ACGEXS_001228"
     CDS             complement(8169..9206)
                     /gene="mutY"
                     /locus_tag="ACGEXS_001228"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFF8012246.1"
                     /translation="MYQQSSFKENLIHWFDENQREMPWRQTTNPYYIWLSEVMLQQTQ
                     VKTVIDYYHRFVERFPTVEVLSQASEDEVLKYWEGLGYYSRARNFHTAIKEVHDKYEG
                     LVPKDPDQFKALKGVGPYTQAAVMSIAYNVPLATVDGNVFRVWSRLNDDYRDIKLQST
                     RKSYEQELLPYVTTEAGTFNQAMMELGALICTPKNPLCLFCPVQENCEAFDKGTFEKL
                     PVKSKNVSKKVIEQSVFLIRNNQGQYLLQKRSEKLLHGMWQFPMFESEHARRKMTEKI
                     GHDIQPVETPIFELKHQFTHLTWKIKVYAVSGAINIETLPDDMIWFDLSDRDQYTFPV
                     PMSKIYQFING"
     gene            9358..10335
                     /locus_tag="ACGEXS_001229"
     CDS             9358..10335
                     /locus_tag="ACGEXS_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFF8012247.1"
                     /translation="MDTATHIAIGVGLTALATQDPAMASTFGATATTLIVGSLIPDGD
                     TVLKLKDNATYISHHRGITHSIPFTILWPILITFLIFTFFSGTNPFHVWMWAQLAVFL
                     HVFVDIFNSYGTQALRPITNKWIQLSVINTFDPIIFTVLCIGIVLWVIGLHPFAVFFP
                     IIALLIIYYMIRFKMRAVIKQQALKAIQQEHHPVKVFVAPTIKFMEWRVAIQTDAHDY
                     VGKAYGRNVVFSDKVERQTLSTDSILWKVKGNKDIRTFLNFSSIYRWQTTTLADGSTE
                     IRLIDLRYLKNDHYSFVAIAHVTNDNVIDHSYIGWVFTEDKLQRKLYAK"
     gene            complement(10596..11432)
                     /locus_tag="ACGEXS_001230"
     CDS             complement(10596..11432)
                     /locus_tag="ACGEXS_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012248.1"
                     /translation="MLDNIILYFKNLPHTKRYVTERLKQSWKSFLIVLAACLILIIAS
                     ETLFSFSHLTDVKEVRWLFRIIVLIVFAVVMFTIYISYHHYMNDFLVTKLFNISAATP
                     VVIMSILSFIMLVILTMISALVKPVNFETSYIALFYFIVMATIFVGLISVTFGLIRLL
                     TEKINIIFYGVCVLCFLLLPIIFIPNPNHVFINHILMLNPMYYIVNGIAQSIIFGISS
                     MENIPYHFYFILFLCLIAAVNFVLARYTTHAIYNKTSKVTQTDNQQDVSNDSTDEADT
                     SS"
     gene            complement(11441..12958)
                     /locus_tag="ACGEXS_001231"
     CDS             complement(11441..12958)
                     /locus_tag="ACGEXS_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFF8012249.1"
                     /translation="MGSSIVLKLLKVTHYYRNKQNKKWYLPFGYDAEDIDLNNISLHI
                     YQGEALGIIGEPESSKALVGQLLAGAIKPDKGKVVCTEDLFYGYIEDQSLIHQTVEAY
                     TAQLVQLFPYEINDHKAEQIIQYAHLGDYKTKPVNHISKAAYAQLLLSIARSSKSNII
                     ILNHVIDYLTPQFMERAIELTNDYIENNLTIVSIGDDIDKISQVSNYIAWFSHGQLRM
                     EGSLKQVIPSFKEHERDRLSLNSKEEIENFDLDWKKNRTRIPEMTYNFKRVERYNHAK
                     PPKFLVRFWTLASGTILGLALMMLLIFNNIGIISITDFTNRATMQNENKDPYGEKLAY
                     GIAFNGSVDMQGDKQVTIPKYSVVTITGENSKNYRVTADNKTYYVSKDKLEYFNPAGL
                     YQTHSFKKLAPYMKSNYSNYYAYFNSQLHKKHSSVIKTLVPDDDNRFVASVTQQPIQL
                     LFNDNNQLYGFVYPIVDKKELKDKFNINNNIWITKVGNGYCIANLKEDKWIYIEL"
     gene            complement(12973..13287)
                     /locus_tag="ACGEXS_001232"
     CDS             complement(12973..13287)
                     /locus_tag="ACGEXS_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhH family protein"
                     /protein_id="HFF8012250.1"
                     /translation="MEQKKLSEMSEPELRHEIQLYKEKMRKAEMNGILNEYDVYQSKV
                     IVAESYLVDRKKIEIGKIYKLTDGSNQYFKVERLKGIFAWGFRFNSDEPEEGLPIALL
                     QL"
     gene            complement(13265..14083)
                     /gene="recX"
                     /locus_tag="ACGEXS_001233"
     CDS             complement(13265..14083)
                     /gene="recX"
                     /locus_tag="ACGEXS_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFF8012251.1"
                     /translation="MPKITKIEVQKKNKERFNLFLDEQFEMGIDIDTLVKFNLKKGQQ
                     LEAADMAEIQKYDHYRIGLNKAIQYLSYKKRTEKEVIQYLQKEEISEQAISEVIEYCY
                     REKLIDHQDYAESLKNTMIRTTDKGPKIYQQKLYQLGIEPNIIEIFTELYREQQELDD
                     IIQIAEKISKTKKGPQNKVKEKVMQSLIQKGFEMETIHAVLNEMDFTQDEAVLDDLLQ
                     RDLEKIYNKNRKKYTQQKLISKTIEGLMRKGYKYDKIKAKLEESGIADGTEEIE"
     gene            complement(14344..15153)
                     /gene="sgtB"
                     /locus_tag="ACGEXS_001234"
     CDS             complement(14344..15153)
                     /gene="sgtB"
                     /locus_tag="ACGEXS_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional peptidoglycan glycosyltransferase
                     SgtB"
                     /protein_id="HFF8012252.1"
                     /translation="MKRSDRYSNSNEHFEHMKHEPHYNTYYQPVGKPPKKKKSKRILL
                     KILLTILIIIALFIGIMYFLSTRDNVDELRKIENKSSFVSADNMPEYVKGAFISMEDE
                     RFYNHHGFDLKGTTRALFSTISDRDVQGGSTITQQVVKNYFYDNDRSFTRKVKELFVA
                     HRVEKQYNKNEILSFYLNNIYFGDNQYTLEGAANHYFGTTVNKNSTTMSHITVLQSAI
                     LASKVNAPSVYNINNMSENFTQRVSTNLEKMKQQNYINETQYQQAMSQLNR"
BASE COUNT         4976 a         2611 c         2116 g         5619 t
ORIGIN      
        1 aagttattct agtagagtca acataaaaca acacttattt ttgtttaaaa attactgttg
       61 aagggattat tttctattcg taagtactat atgtatttaa agtgtaattc cttatgcttt
      121 ttacttcagt tttacaaaca aacttatact cactttatgg atagttattt agtagacctt
      181 cgtttgaata tttttatgtt tatttataaa ggatactagt tattagttaa taccacttcc
      241 gtagttagaa tattcttttc ttcaatttaa atatttttca gaataagcat aaaaaaagtt
      301 aaacaacttt catgtcgttt aacttcttaa atattacgct tacttatatg aagtaagcta
      361 acgctttttt aatttgtcat actaccaaag ttaaattatt tatcttggca ttctttacaa
      421 actccataaa tttccattcg atgatgtgtt acgtcaaagt cagtcatatg ctgagctaat
      481 ctttcaattt catttaactg tggatattga aaatcaacaa tcttaccaca ttgttcacat
      541 ataatatgat aatgattatg tgtattaaag tcgaatcgac ttgatgagtc tccatatgtt
      601 aattctttta caattccaat atctttaaac actcttaagt tattatatat tgtcgcaaca
      661 cttatatttg gaaaatcagg tgaaagtgct tgataaattt catcagctgt tggatgagta
      721 tgtgaagaaa ttaaataacg taatattgct tgtctttgag gtgtaattct tacgcctgct
      781 tgtcgcaatg atgcaattga ttcttctagt tcatgttcaa ttgattctat ttcaacactc
      841 atctatatca ccatctttct tatataataa ttattattac ttactataaa tataactgta
      901 gtattgcgcg cgtgtcaata tttacatgat gatttcattt aatagccttt tttagcatca
      961 acttcattct caattagacc attcttattg agaaaattaa ctagattgtt tttaaaaata
     1021 tctaataagt catactttgc ttcataatca ttaccagtta tatgcgctgt tatagttaca
     1081 ttatccaatt catataattc atgattaggt ttcaaaggtt cattttcaaa cacatctaaa
     1141 tatgcatgtc gaataacttt actttttaat acttctatta agagcgcttc tttaactatg
     1201 ctacctcgtc ctatatttat aaaaagtgct tcatctttca ttaattcaaa atgttttttc
     1261 tttagtaaat gaatcgtttc ttgcgtttct ggtaaagcat ttataataat gtcagcattt
     1321 ggtaatgtgc tttcaagtga ttcgatagta tatatctcgt caaactcatc tttgttctga
     1381 cctgacttgc tcagaccaat taaattcata ttaaaagcct ttgctaactt cgcagttcta
     1441 gtagcaattg cacctgtacc taaaaataaa actgtttgtc ctgataggcg tttaccagtt
     1501 attttcgaat catatatatg ttgtcgttgg ttatcatatg atagtttcat ctttttataa
     1561 tcatctaaaa tgaaagctaa aatgtattca gataattgtt tagcttgaac accttttcca
     1621 ttagttaaaa gtatgccgtg atctgcaata taatctaacg gcaatgtatt tacacccgtt
     1681 gcaaaccatg caatccattt taaattcggg caacgtcgta aaaatgcctc attgataccg
     1741 ccatcataac caactaatat atctaaatca gccaagtcgc tctcaggtat ttctgatgct
     1801 tttttataaa atttaaaatc taaatctgaa aagcgttgtt gtaattcagt ttcaacttca
     1861 cgcatacgat ttaacccaac aactttcata tatgtcaccc caaaatgttt ttaagttctt
     1921 ctatttgtgt tttaacctta accttctcga taacatctaa tactttacct tgttcatcta
     1981 ttataaaagt cgttcttaca atgcccatac tttctttgcc aaatgatttt tttaactgat
     2041 atacgccagt ttctttagct aatttaaaat cttcatctac taataaatcg aaattcaatc
     2101 cgtgtttctc aataaaattt tggtgttttt tctttgaatc accgcttata ccatatactg
     2161 caacatctaa atcattgaac atttctaaat tgtctctaaa gtcacaagct tctgtggtac
     2221 aagtaggtgt attatctcta ggataaaaat atataatcgc ctttttacct tttaatgtat
     2281 catttgtaat gacagttccg tcttgatttt ctaatttaaa tattggaaat tgttctcctt
     2341 tttgcaacat ataatcacct ttttcttcat tataataatt ttatgatacg atattagatg
     2401 aaaatattaa attaaaagag gttgatataa atgaatttta gtgaaagtga acgtttacaa
     2461 caactttcaa acgaatatat tctaggcggt gtcaattccc cttctcgttc ttataaagct
     2521 gtaggaggcg gtgcacctgt tgttatgaaa gaaggacacg gtgcatattt atatgatgtc
     2581 gatggcaata aatttattga ttaccttcaa gcatacggtc caattattac ggggcatgca
     2641 catcctcata ttactaaagc aattcaagaa caagctgcta aaggtgtttt atttggtaca
     2701 ccgactgaat tagaaattga attcagcaaa aaattacgtg atgcaattcc atctcttgag
     2761 aaaattcgct ttgtaaattc tggaacagaa gcagtcatga caacaattcg tgttgcacgt
     2821 gcatatacta aaagaaataa aattataaaa tttgctggat cttatcatgg ccattctgat
     2881 ttagtattgg ttgcagcagg tagcggccca tctcagctcg gttctccaga ctcagctggt
     2941 gttccagaaa gcgtcgcacg tgaagtcatt actgtacctt tcaatgatat taacgcctat
     3001 aaagaagcaa ttgaattttg gggtgatgaa attgccgcag tattagtaga accaattgtt
     3061 ggtaactttg gaatggtaat gcctcaacct ggatttttag aagaggttaa tgaaatttca
     3121 cataacaatg ggaccctagt gatttatgat gaagtaatta ctgcattccg tttccattac
     3181 ggtgccgctc aagatttatt aggtgttatc cctgatttaa ctgcatttgg taaaattgtt
     3241 ggcggtggtt taccaattgg aggctatggt ggacgtcaag atattatgga acaagtagca
     3301 cctctaggac ctgcatatca agctggtaca atggctggta acccgttatc tatgaaagca
     3361 ggtattgcat tactcgaagt actagaacaa gacggtgttt atgaaaaatt agacagctta
     3421 ggccaacaac tagaagaagg tttacttaaa ttaatcgaaa aacataatat cacagctaca
     3481 attaatcgta tttatggatc tttaacattg tactttacag atgaaaaagt cacacattat
     3541 gatcaagttg aacattctga cggcgaagcg ttcggtaaat ttttcaaatt aatgttaaat
     3601 caaggtatca atttagcacc ttctaagttt gaagcttggt tcttaacaac tgaacataca
     3661 gaagaagata ttaaacaaac tttaaaagct gcagactatg cttttagtca aatgaaataa
     3721 cttttaagga agtatatttt taattgaatt aaaataaagc aaatgctcac atagtagatt
     3781 cattttatat cgttaactat taacgtttaa cttgatatgt cactatgtga gtttttattt
     3841 tacaaattta tactatttta atctctgact aaagttttac atcgctatca gtattatgta
     3901 tgatttattt taacaactat aaataagctt gaattttgta actagtaagt gtataacagt
     3961 aatgaatacg aatattagat attcaataaa cttaaaaggg gcgtcattat ttttgagact
     4021 aggagctcgg atttttaaaa ctggtatagc cattatttta gctatgtcta tcgcttcttt
     4081 actaccggat gatgttggtc tgaaagcctt agctggtgtc agtgctgttg ttgcaatgca
     4141 acctagtatt tatcgttcat ttaaaacagt ttctgatcaa gcattgggta acattatagg
     4201 tgccatacta tctgttacaa tggtaacaat ttttagtgat aattttatca ttatgggtgt
     4261 taccgtcata gtacttattg ctattttatt taaatttaat ttagcacatg ttgctacttt
     4321 ggcgagtgta acagcattaa tcattatggg acaacatacc ggttctttct atattaccgc
     4381 cttttacagg tttgtacttg taatgattgg tgtcataagt tcatctctag ttaactttgt
     4441 gtttctacca cctaaatttg aaacaaaaat ttactataat tcattaaata tatcctcaga
     4501 tatttttatg tggtttaaat tagtattaaa tgatacgacg gagtttaata atattaaaca
     4561 agatagtcat aatttaaaac aacgtgtcga aaagttagag aaaatttatg attactatag
     4621 cgaagaacgt cctattacaa aaaaacatat tcaccaacag aatagaaaga aaatactttt
     4681 cagagaagta gttcagacga ctagacaagc ttatgaagta ttaaacaaat tgtcgcgtta
     4741 tcaaaacgac ttatatttac ttaataacaa tttcctatta caaatcaaat tagatttaga
     4801 ttcattaact gcttttcacg agcaaatttt agctagcctt tctaaaaaag cacgttataa
     4861 tgtgacacat gttgattatg agttagataa tccgcagaaa aaagatttat tgtccacatt
     4921 tcagcatgag ttaataaatc atccttatca aacggaatat tcgtttgcca atgttatgca
     4981 aattgttgca gccatcgaag agtatcgaca ccatttagag catttagacc gcataagaat
     5041 tagctttttc acatatcata gatctgatgc agacattgaa attgttgaag aagactttga
     5101 tttataacat taaaacaatt ttatcaatta atgccacaat gagataaact aatattgaat
     5161 ctcgaaatta aatttaaaaa agcagtaaga ttattttcaa ttaagaaaat ctcttactgc
     5221 tttttctatg ttatgacccc accatcctgt tacaaatttt ctcttcaaga ttgtactgaa
     5281 gtcaaacaac taatacttta taagttttga atgctatata aatgctcgta agcaccttgt
     5341 tttgcaatca attcacgatg cgtacctgtt tcaacaatat gtccattttc aattacgaca
     5401 attttgtcag catgtgtaat agtggacaag cgatgcgcta cgataagtgt cgttcgatct
     5461 ttactcaaca catctaatgc ttcttgaata atggattcac tttctaaatc aagtgcactt
     5521 gttgcttcat ccaagataag aattggcgga ttatttaaaa atattctagc aatcgataat
     5581 ctttgttttt gaccacctga taatttaaca cctcgttcac ctacttcagt gtcatatccc
     5641 tgtggcaagt tcataataaa gtcatgtgca ttagccattt tcgccgcttc aactacttct
     5701 tcatctgttg ctgttggacg accaagtaaa atattttcct taactgtatc agagaataaa
     5761 atattatcct gttgcaccaa tcctatttga tttcttaaac ttcccgttaa aaaatcttta
     5821 atgttgtgac catctattaa aatttgccca gaagttacat cgtaaaatct cggtattaag
     5881 ttaattaatg ttgatttacc accaccactc atacctacga aagcaactgt ttctcctttt
     5941 tcaatactca aattaatatc ttttaaaatt ggagcttcgt tatcgttata ttgaaaacta
     6001 acatgatcaa tatcaatacg accttgttta atttcaatag gttgagcacc aacaccattt
     6061 ttgatgtcat aatcttcatc aattaattgg aatacacggt ccattgaagc aaaactttgc
     6121 gttaaagttg taaatgatgc gactaaacga cgtaaaggcc cgaacaataa ctctaagtat
     6181 ccaacaaatg ctgcaagtgt acctactgtg attgatccag aaatagcaag atatgcacca
     6241 acaccgatga caataattgg tccaatatct gtaactgtat taattgcggc aaaggaatag
     6301 gcattccatc ttgtatgttt caacgcacgt gttaggaaat tagtattctt tttatcaaag
     6361 tttttcgctt cattgtcttc aatcgcaaaa cttttaacga ctgaaatacc ttgaacacgt
     6421 tcatgcaaga atccttgaac ctcagctaat gcttgagatc tttcacgtgt caattttctt
     6481 aatcttccaa agaaaacgta caccgttaaa atgtaaaatg ggaagataaa cagtgctgct
     6541 aaagtcaatt tcacatctaa aaagaacatt atggatagtg caataataat tgttatacaa
     6601 tctaaccaaa tattcattaa cccggttaaa atgaaatctt ttgtttgttc aacatcatta
     6661 atcactctag atattacttg acctacttga ttattagcat aaaatctcgc acttaaagct
     6721 tgtaaatggt tgtataactt tttacgtata tcatacaata ttttattact tgtccattgc
     6781 gccaaatatt gacgtataaa ttcaattggt ggtctcacta ttacaaaaat aaataatgcg
     6841 ataccaatgg caatagttaa atgatgaact ttttcatcag tcgttagtgc gtggttatta
     6901 atcacgccat ctattgcata tttaattaat agtggtataa gcattggtat accaaactta
     6961 attatcccaa caataatcgt tgcaaaaata cgatatttgt atggcttaac aaattgcaaa
     7021 tatcgtttaa tcatacaatc ccccctaatc tattgcccta tcctattcat aagcataaaa
     7081 atgaatagag gttggataca taatttgtag atgtaaattc ttcttacaat ttacattttt
     7141 aaaattaaga tatctcaacc tctgtcatat tcatgtgatt aaatacgttg tcttattcgt
     7201 atgttatatt ctattcaatt taatctatgg atactgtgtc cccacacgac agcaaaagtt
     7261 atcatacttc tttacatcac taagtcaata taaatgattt aatcagtatt tacactttat
     7321 ttgcttaata ctgtctaatt tttttgtaac gttctttcca aactttgata aaatctggcg
     7381 cgaatgggcc cttcttctgt tctatccatt gttgaagaat gtccacgttg cgtcttaaaa
     7441 taatatcaat atcatgcgga taattcattt gattcatatg ttgctcatat tcatcttcat
     7501 ctaataaatg atactttccg tttggatata ctttaatatc taaatcatag tctatatatt
     7561 ttaatgcctc ttcatcacaa acaaatggtg atgacaaatt gcaatagtaa taaattccat
     7621 cttctctaaa catgcagata acattaaacc aatattctga gtgaaagtaa acaattgccg
     7681 gttcacgtgt tatccaagtt cttccgtcac tttcagtcac taacgtatga tcatttccac
     7741 caatgacaac atgatcagta ccctttaata ttgttgtttc agaccaaacg cgatgaatct
     7801 taccatcatg tttataactc tgaattttaa tgttttcccc ttctttaggt atggattctc
     7861 tgaccatact ccacaccacc ttctgttaat ttaaccatta taaattatag catatttcag
     7921 aaatagtatt atataaatac atatttttac gaaataagat tttactactt aataattaaa
     7981 ctcggtaata ttgctaagta ctacaacaga gatttacatg tcccatttaa agtatataaa
     8041 atcatcactt ttatatatca acactttaac tttttgacat tgttattcta tgagatttaa
     8101 agatatcatt tatacttttt aaaattaatg tcactatgtt ttccgataat attaccaatc
     8161 atcgaatgtt acccatttat aaattgataa atctttgaca taggtacagg gaatgtatat
     8221 tgatctcgat cacttaaatc aaaccaaatc atgtcatctg gtaatgtttc aatgttaatt
     8281 gctcctgaaa cggcgtatac tttaatcttc catgttaaat gagtaaattg atgcttcaac
     8341 tcaaaaatag gtgtttctac tggttgaatg tcatgaccga ttttttcagt cattttacgt
     8401 ctagcatgct cactttcaaa cataggaaat tgccacatac catgcaataa tttttcgcta
     8461 cgcttttgca acagatattg accttgatta tttctaatta aaaagacgga ttgctcaatt
     8521 acttttttac ttacattttt agatttaaca ggtaactttt caaatgtacc tttatcaaat
     8581 gcctcacagt tttcttgaac tggacaaaat aagcataatg gattttttgg tgtacaaatt
     8641 aacgccccta attccatcat agcttgatta aacgttccag cttctgtagt aacatacggt
     8701 aataattctt gttcgtacga tttcctcgtc gattgtaatt taatatctcg atagtcatca
     8761 ttcaatctag accatactcg aaaaacattt ccgtctacag ttgctagtgg tacattatat
     8821 gcaatgctca ttactgcagc ttgtgtgtat gggccaacac cttttaacgc tttaaattga
     8881 tcaggatctt tgggaactaa gccttcatat ttatcatgaa cttctttaat cgccgtatga
     8941 aaatttcgag ctctactata atatcctaag ccttcccaat actttaacac ttcatcttcc
     9001 gaagcttgac tcaaaacttc cacagttgga aatcgttcaa caaaacgatg ataatagtca
     9061 ataactgttt taacttgtgt ctgttgtaac atgacctcac ttaaccaaat atagtacgga
     9121 ttggtcgttt gtcgccatgg catttctctt tgattttcat caaaccagtg tatcaaattt
     9181 tctttaaaac tagactgctg atacatttat aaaacccttt cctcaccaaa attaattgtc
     9241 tttactcata atgtttttat tgtacattaa aatcatggtt agtatgtaag ttaatttagt
     9301 tatttgcgaa attggattat aatagtatat ataatattat gaaatgagtg aactgatatg
     9361 gacactgcaa cacatatcgc aattggggtg ggccttacag cacttgcaac tcaagatcca
     9421 gcaatggctt ctacgtttgg tgcaacagct acaaccctta tcgttggttc attaattcct
     9481 gatggggata ctgttcttaa attaaaggac aatgcaacat atatttcgca tcatagaggt
     9541 atcacgcatt ccatcccttt cacaatacta tggccaattt taattacatt tttaatattc
     9601 acgttcttta gtggaaccaa cccatttcat gtatggatgt gggctcagct cgcagtattt
     9661 ttacatgtct ttgtagatat attcaattct tatggtacac aagcgcttag acctatcaca
     9721 aacaaatgga ttcaattaag tgtgattaac acatttgacc ctattatttt cacagttctt
     9781 tgtattggta ttgtattatg ggttataggc ttgcatccat ttgcagtctt ctttcctata
     9841 atcgctttac taatcattta ttacatgatt cgttttaaaa tgagagccgt aattaagcaa
     9901 caagctttaa aagcaattca acaagagcat caccctgtta aagtatttgt tgcgccaaca
     9961 ataaaattta tggaatggcg tgtcgcgata caaactgatg cacatgacta tgttggaaaa
    10021 gcatatggta ggaatgtggt gtttagtgat aaagtggaac gtcaaacatt atcaacagac
    10081 tccattttat ggaaagtcaa aggtaataaa gatatacgta cttttttaaa cttttcatca
    10141 atctatcgtt ggcaaacaac aacgttagca gatggttcta ctgaaattcg tttgattgat
    10201 ttgcgttatt taaaaaatga tcattattca tttgtggcaa ttgcacatgt aacaaacgat
    10261 aatgtcatag accactctta tattggctgg gtatttacag aagataagtt acaacgtaaa
    10321 ctgtatgcta aataatttca agttattatt cactaaagtt aatctataaa aaatgaacaa
    10381 ccgggcagaa tgaaaatcaa aacgattttt actctgtccg gttttttaat gtaaaactat
    10441 gaatgctttt acaaaatcta aaatttatat tgttgctaac aaactacctt taatgactcg
    10501 aaatatcaaa atcagtatag gaaaacaata tctagatgat attctaattg tttctgattc
    10561 tcacagatta atttacacaa caggtcagct aaacatcatg aagaagtatc cgcctcgtct
    10621 gtactatcat ttgaaacatc ctgttgatta tcagtttgtg tcactttaga tgttttatta
    10681 taaattgcgt gtgtcgtata ccttgctaat acgaaattta ctgcagctat taaacataag
    10741 aataaaataa agtaaaaatg atatggaatg ttttccatac ttgatatacc aaatatgata
    10801 gattgtgcta taccattaac aatgtaatac attggattta gcattaggat gtgattgata
    10861 aatacatgat ttggatttgg tatgaaaata attggtaaca ataagaaaca caatacacaa
    10921 accccataaa atatgatatt tattttttca gttaacagtc gaataagacc aaaagtaacg
    10981 gatattaatc ctacaaaaat agttgccatc acaataaaat agaatagcgc tatatatgat
    11041 gtttcgaagt ttactggttt aaccaatgca ctaatcatcg tcaaaatgac tagcataata
    11101 aaacttaaaa tagacataat aactactggc gtcgctgcgg aaatattaaa taacttagta
    11161 actaaaaagt cattcatata atgatgataa gagatataaa ttgtgaacat cactaccgca
    11221 aaaacaatca atacaatgat tctaaataac catctcacct ctttaacatc ggtcaaatga
    11281 ctaaaggaaa atagcgtttc acttgcaata ataagtatta aacatgcagc taatacgatt
    11341 agaaaacttt tccaactttg ttttaaacgt tctgttacat accttttagt atgcggtaag
    11401 tttttaaaat ataaaataat attatctagc atctttacac ctacaattca atataaatcc
    11461 atttgtcttc tttcaaattg gcaatacaat atccattccc aactttagta atccaaatgt
    11521 tattgttaat attaaactta tcttttaatt cttttttatc tacaattgga taaacaaaac
    11581 cgtataactg attattatca ttgaaaagta attgtatcgg ttgttgtgta acggatgcaa
    11641 cgaaacggtt atcatcatca ggaactaaag tttttataac tgaactatgc tttttatgta
    11701 attgactatt aaagtatgca tagtaattac tataatttga tttcatatat ggtgctaatt
    11761 ttttaaaact atgcgtttga tataaacctg ccgggttaaa atattctaat ttatctttac
    11821 taacatagta agtcttatta tcggcggtaa cacgataatt tttactattt tcgccagtaa
    11881 ttgtaactac actatatttt ggaattgtga cttgtttatc cccttgcata tccacactgc
    11941 cattaaaagc aattccataa gctaactttt cgccatatgg atctttattt tcattttgca
    12001 tcgtagcacg attcgtaaaa tctgttatcg aaataattcc tatattattg aaaatgagca
    12061 acatcatcaa tgctaagcct aaaatagtac ctgaggctaa agtccaaaaa cgcactaaaa
    12121 acttaggcgg ttttgcatga ttatagcgct caacacgttt gaaattatag gtcatctctg
    12181 gtattcttgt acgattcttt ttccaatcta aatcaaagtt ttcaatttct tcttttgagt
    12241 ttagacttaa tcgatcacgt tcatgttctt taaaagatgg aataacttgt ttaagtgacc
    12301 cttccattct taattgacca tgtgaaaacc aagctatgta gttactcact tgtgaaattt
    12361 tatcaatatc atcaccaatt gacacaatcg ttaaattatt ttcaatataa tcatttgtta
    12421 attcaatcgc acgttccata aattgtggtg ttaaatagtc aataacatga tttaaaataa
    12481 taatatttga ttttgatgag cgtgcaatac ttaatagtaa ttgagcgtat gccgctttcg
    12541 aaatatggtt aaccggcttc gttttataat cacctaaatg tgcatattga ataatctgtt
    12601 cagctttatg atcattaatt tcatatggaa atagttgaac taactgcgct gtataagctt
    12661 caacagtttg atgaattaac gattgatctt caatatatcc gtagaacaaa tcttcagtac
    12721 aaactacttt acctttatca ggtttaattg cacctgccaa caattgacct accaacgctt
    12781 tggaagattc aggttcacca attataccta atgcttctcc ttgataaata tgtaaactaa
    12841 tattgtttaa atcgatatct tcagcatcat atccaaaagg taaataccat ttcttattct
    12901 gtttattcct atagtagtgt gttactttta gtaactttaa aacaattgaa cttcccatct
    12961 attttcatcc ttctataatt gtaataacgc tataggtaat ccttcttcgg gttcatcgct
    13021 attaaatcta aacccccacg caaaaatacc ttttaatctt tctactttaa aatattgatt
    13081 actgccatca gtcagtttat aaattttacc aatttctatc ttttttctgt ctactaagta
    13141 actttcggca acaattactt tactttgata aacgtcatat tcatttaaaa taccattcat
    13201 ctcagctttt ctcattttct ctttataaag ttgtatttcg tgtcttaatt ctggctcaga
    13261 catctcactc aatttcttct gttccatcgg caataccact ttcttctaat ttagctttaa
    13321 ttttatcata tttatatcct tttctcataa ggccttcgat agtttttgaa attaatttct
    13381 gttgcgtgta cttctttcga tttttattat aaattttttc taaatctcgt tgtaataaat
    13441 cgtctaaaac cgcttcatct tgtgtaaaat ccatttcatt caatacagca tgaatggttt
    13501 ccatttcaaa ccctttttga attaaagatt gcattacttt ttctttaact ttattttgtg
    13561 gccctttttt tgttttagat attttttcag caatttggat aatatcatca agttcctgtt
    13621 gttctctata aagttctgta aatatttcaa tgatatttgg ttctatacca agttgatata
    13681 gtttttgttg ataaatttta ggtcctttat ctgtcgtgcg aatcattgta ttttttaaac
    13741 tttccgcata atcttgatgg tcgattaact tttcgcgata acaatattca atcacttcag
    13801 aaatcgcttg ctctgatatc tcttcttttt gtaaatattg tataacttct ttttcagttc
    13861 tctttttata tgataaatat tggattgctt tatttaaacc tatgcgataa tgatcatact
    13921 tttgaatctc tgccatgtca gcagcttcaa gttgttgccc tttttttaaa ttaaatttga
    13981 ctaatgtatc aatatctata cccatttcaa attgttcgtc taaaaaaaga ttaaaccgtt
    14041 ctttgttttt cttttgaact tctattttag taatcttcgg cactggatca ccccgttact
    14101 ttcttaagtt ttaatacaat actcttttat tattcaataa gccacttcct atagcaaatg
    14161 tttaacttta aatatttttc gatgctaaca aaaaatcaca ctatcatctt ttaaaatgaa
    14221 agtgtgatta caagcaaatc tgtaaaattt ataaagcaga aacaattcaa ctttatcatt
    14281 atgacatttc aattaaacct tctacattat agttccaagc atcttacaca tgaatgcaag
    14341 tatttaacga tttaattgtg acatagcctg ttgatattgt gtttcattga tataattttg
    14401 ttgcttcatt ttttctaagt tcgtgcttac acgttgcgta aaattctctg acatattatt
    14461 gatattatat acgctaggtg cattgacttt actagctaaa atagcgcttt gtaaaactgt
    14521 tatgtgagac attgttgtac tatttttatt cacggttgtt ccaaagtaat ggtttgctgc
    14581 gccctcaagc gtatattgat tatccccaaa gtaaatatta tttaaataaa agcttaaaat
    14641 ttcgttctta ttatattgtt tttcaactcg atgagctaca aataattctt ttacttttct
    14701 agtaaatgaa cgatcattat cataaaaata atttttgaca acttgttgtg taatggtact
    14761 accaccttgc acatctctgt cgctaatcgt tgaaaataaa gctctagttg tacctttcaa
    14821 atcgaatcca tgatgattgt agaatcgttc atcttccatt gaaataaagg cacctttaac
    14881 atactctggc atgttatcag ctgacacaaa actactttta ttttcaattt ttcttagttc
    14941 atccacatta tcgcgtgtag ataaaaaata catgatacca ataaacaatg cgataatgat
    15001 tagaatggtt aataatattt ttaatagtat tcgtttactt tttttctttt tcggcggttt
    15061 gccaactggt tgataatacg tattatagtg aggttcgtgt ttcatatgct caaaatgttc
    15121 atttgagttt gagtacctat cgcttctttt catgcgtttg ctccttcttt taaaactcac
    15181 ttagtatata ccttgagttt accagtacta tcacaaatag gctacacttt ttgggaaaat
    15241 cagtccaagg gcttacaatc gtatacgcca tcatacttac ttttttgttt tttgaaaaaa
    15301 ttatagataa atcattgcaa tt
//
LOCUS       DAXWFK010000051        15256 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.051, whole genome shotgun sequence.
ACCESSION   DAXWFK010000051 DAXWFK010000000
VERSION     DAXWFK010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15256
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.051"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(46..1341)
                     /gene="pbp4"
                     /locus_tag="ACGEXS_001235"
     CDS             complement(46..1341)
                     /gene="pbp4"
                     /locus_tag="ACGEXS_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP4"
                     /protein_id="HFF8012253.1"
                     /translation="MKNLISIIIILCLTLSIMTPYAQATNSDVTPVQAANQYGYAGLS
                     AAYEPTSAVNVSQTGQLLYQYNIDTKWNPASMTKLMTMYLTLEAVNKGQLSLDDTVTM
                     TNKEYIMSTLPELSNTKLYPGQVWTIADLLQITVSNSSNAAALILAKKVSKNTSDFVD
                     LMNNKAKAIGMKNTHFVNPTGAENSRLRTFAPTKYKDQERTVTTARDYAILDLHVIKE
                     TPKILDFTKQLAPTTHAVTYYTFNFSLEGAKMSLPGTDGLKTGSSDTANYNHTITTKR
                     GKFRINQVIMGAGDYKNLGGEKQRNMMGNALMERSFDQYKYVKILSKGEQRINGKKYY
                     VENDLYDVLPSDFSKKDYKLVVEDGKVHADYPREFINKDYGPPTVEVHQPIIQKANTV
                     AKSMWEEHPLFTIIGGTCLVAGLALIVHMIINRLFRKRK"
     gene            1762..3489
                     /locus_tag="ACGEXS_001236"
     CDS             1762..3489
                     /locus_tag="ACGEXS_001236"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8012254.1"
                     /translation="MKRENPLFFLFKKLSWPVGLIVAAITISSLGSLSGLLVPLFTGR
                     IVDKFSVSHINWNLIALFGGIFVINALLSGLGLYLLSKIGEKIIYAIRSVLWEHIIQL
                     KMPFFDKNESGQLMSRLTDDTKVINEFISQKLPNLLPSIVTLVGSLIMLFILDWKMTL
                     LTFITIPIFVLIMIPLGRIMQKISTSTQSEIANFSGLLGRVLTEMRLVKISNTERLEL
                     DNAHKNLNEIYKLGLKQAKIAAVVQPISGIVMLLTIAIILGFGALEIATGAITAGTLI
                     AMIFYVIQLSMPLINLSTLVTDYKKAVGASSRIYEIMQEPIEPTEALEDSENVLIDDG
                     VLSFEHVDFKYDVKKILDDVSFQIPQGQVSAFVGPSGSGKSTIFNLIERMYEIESGDI
                     KYGLESVYDIPLSKWRRKIGYVMQSNSMMSGTIRDNILYGINRHVSDEELINYAKLAN
                     CHDFIMQFDEGYDTLVGERGLKLSGGQRQRIDIARSFVKNPDILLLDEATANLDSESE
                     LKIQEALETLMEGRTTIVIAHRLSTIKKAGQIIFLDKGQVTGKGTHSELMASHAKYKN
                     FVVSQKLTD"
     gene            3848..5077
                     /locus_tag="ACGEXS_001237"
     CDS             3848..5077
                     /locus_tag="ACGEXS_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFF8012255.1"
                     /translation="MFLLINIIGLIVFLGIAVLFSRDRKNIQWQSIGILVVLNLFLAW
                     FFIYFDWGQKAVRGAANGIAWVVQSAHAGTGFAFASLTNVKMMDMAVAALFPILLIVP
                     LFDILMYFNILPKIIGGIGWLLAKVTRQPKFESFFGIEMMFLGNTEALAVSSEQLKRM
                     NEMRVLTIAMMSMSSVSGAIVGAYVQMVPGELVLTAIPLNIVNAIIVSCLLNPVSVEE
                     KEDIIYSLKNNEVERQPFFSFLGDSVLAAGKLVLIIIAFVISFVALADLFDRFINLIT
                     GLIAGWIGIKGSFGLNQILGVFMYPFALLLGLPYDEAWLVAQQMAKKIVTNEFVVMGE
                     ISKDIASYTPHHRAVITTFLISFANFSTIGMIIGTLKGIVDKKTSDFVSKYVPMMLLS
                     GILVSLLTAAFVGLFAW"
     gene            5332..5424
                     /locus_tag="ACGEXS_001238"
     CDS             5332..5424
                     /locus_tag="ACGEXS_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012256.1"
                     /translation="MFCYYKQHKGDNFSIEEVKNIIADNEMKVN"
     gene            5602..6435
                     /locus_tag="ACGEXS_001239"
     CDS             5602..6435
                     /locus_tag="ACGEXS_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFF8012257.1"
                     /translation="MNKTVKDLILVVLGSFIFAAGVNAFIISGNLGEGGVTGLAIILY
                     YAFHISPAITNFLVNAVLIAIGYKFLSKRSMYLTILVTILISIFLSLTESWQVETGNS
                     IVNAIFGGVSVGLGIGVIILAGGTTAGTTILARIATKYLDVSTPYALLFFDMIVVAIS
                     LTVIPLDKVLVTVISLYIGTKVMEYVIEGLNTKKAMTIISTNPDKLAKAIDEQIGRGL
                     TILNGHGYYTREEKDVLYVVISKTQVSKAKRLIKQIDKDAFLVIHDVRDVYGNGFLAD
                     E"
     gene            6717..7514
                     /locus_tag="ACGEXS_001240"
     CDS             6717..7514
                     /locus_tag="ACGEXS_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8012258.1"
                     /translation="MNRLHGQQVKIGYGDNTIINKLDVEIPDGKVTSIIGPNGCGKST
                     LLKALSRLLAVKEGEVFLDGENIHTQSTKEIAKKIAILPQSPEVADGLTVGELVSYGR
                     FPHQKGFGRLTAEDKKEIDWAMEVTGTDTFRHRSINDLSGGQRQRVWIAMALAQRTDI
                     IFLDEPTTYLDICHQLEILELVQKLNQEQGCTIVMVLHDINQAIRFSDHLIAMKEGDI
                     IATGSTEDVLTQEILEKVFNIDVVLSKDPKTGKPLLVTYDLCRRAYS"
     gene            7550..8554
                     /locus_tag="ACGEXS_001241"
     CDS             7550..8554
                     /locus_tag="ACGEXS_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFF8012259.1"
                     /translation="MTNRENPTPLKFLSYIIGLSMILLITLFISTLIGDAKIQASTII
                     EAIFNYNPSNQQQNIINEIRIPRNIAAVIVGMALAVSGAIIQGVTRNGLADPALIGLN
                     SGASFALALTYAVLPNTSFLILMFAGFLGAILGGAIVLMIGRSRRDGFNPMRIILAGA
                     AVSAMLTALSQGIALAFRLNQTVTFWTAGGVSGTTWSHLKWAIPLIGIALFIILTISK
                     QLTILNLGESLAKGLGQNVTMIRGICLIIAMILAGIAVAIAGQVAFVGLMVPHIARFL
                     IGTDYAKILPLTALLGGILVLVADVIARYLGEAPVGAIISFIGVPYFLYLVKKGGRSI
                     "
     gene            8551..9567
                     /locus_tag="ACGEXS_001242"
     CDS             8551..9567
                     /locus_tag="ACGEXS_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFF8012260.1"
                     /translation="MISSNNKRRQLIALAVFSILLFLGCTWSITSGEYNIPVERFFKT
                     LIGQGDAIDELILLDFRLPRMMITILAGAALSISGAIVQSVTKNPIAEPGILGINAGG
                     GFAIALFIAIGKINADNFVYVLPLISILGGITTALIIFIFSFNKNEGVTPASMVLIGV
                     GLQTALYGGSITIMSKFDDKQSDFIAAWFAGNIWGDEWPFVIAFLPWVLIIIPYLLFK
                     SNTLNIIHTGDNIARGLGVRLSRERLILFFIAVMLSSAAVAVAGSISFIGLMGPHIAK
                     RIVGPRHQLFLPIAILVGACLLVIADTIGKIVLQPGGVPAGIVVAIIGAPYFLYLMYK
                     TKNV"
     gene            9801..10769
                     /gene="dhaK"
                     /locus_tag="ACGEXS_001243"
     CDS             9801..10769
                     /gene="dhaK"
                     /locus_tag="ACGEXS_001243"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFF8012261.1"
                     /translation="MMKKLINKKETFLTDMLEGLLIAHPELDLIANTVIVKKAKKEHG
                     VAIVSGGGSGHEPAHAGFVAEGMLDAAVCGEVFTSPTPDKILEAIKAVDTGDGVLLVV
                     KNYAGDVMNFEMAQELAEMEGINVQTVIVRDDIAVTNEVQRRGVAGTVFVHKLAGYLA
                     EKGYSLTEIKSRVEALLPEIKSIGMAIEPPLVPTTGKYGFDIEDDKMEIGIGIHGEKG
                     IHREEVKDIDHIVGTLLDELYKEVTANDVILMVNGMGGTPLSELNIVTKYIQQNLAAR
                     TVNVAKWFVGDYMTSLDMQGFSITIVPNKPEYLEAFLAPTTSQYFK"
     gene            10811..11395
                     /gene="dhaL"
                     /locus_tag="ACGEXS_001244"
     CDS             10811..11395
                     /gene="dhaL"
                     /locus_tag="ACGEXS_001244"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFF8012262.1"
                     /translation="MKVNDMKARLLNLEETFKKHESELTELDRAIGDGDHGVNMVRGF
                     SSLKDKLDDSSMQSLFKSTGMALMSNVGGASGPLYGFSFVKMSAVTKDDMDNQDFITL
                     IQAFAEAVESRGKVTLNEKTMYDVVARAAEKLKNGETLTFNDLQQLADNTKDMVATKG
                     RAAYFGEESKGYIDPGAQSMVYILNALIGDEDNA"
     gene            11388..11750
                     /gene="dhaM"
                     /locus_tag="ACGEXS_001245"
     CDS             11388..11750
                     /gene="dhaM"
                     /locus_tag="ACGEXS_001245"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFF8012263.1"
                     /translation="MPKIILVSHSKEIASGTKSLLKQMAGDVDIIPIGGLPDGSIGTS
                     FDIIQEVLTKLEDDALCFYDIGSSEMNVDMAIEMYDGNHRVLKVDAPIVEGSFIAAVK
                     LSIGGSIDDALAEIKQSF"
     gene            11866..12363
                     /locus_tag="ACGEXS_001246"
     CDS             11866..12363
                     /locus_tag="ACGEXS_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012264.1"
                     /translation="MQHLIKKHVLNGEFDLVRQLMSETDFMEFEEAYISSAHEVESMM
                     FYTCILDMIKYEESSEMHDLAFLLLVYPLSEYEGALDSAYYHADASIKLTDGKEVKSL
                     LQMLLLHAIPTPVISDKKAFDIAKQILKLDPNNNVARNVLKDTAKRMDNVVVDINELH
                     QRNAR"
     gene            12819..13886
                     /locus_tag="ACGEXS_001247"
     CDS             12819..13886
                     /locus_tag="ACGEXS_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012265.1"
                     /translation="MMMNKEATKIGFAYVGIVVGAGFSTGQEVMQFFTKYGLWAYLGV
                     IISGFILAFIGRQVAKIGTAFEATNHESTLQYVFGEKFSKVFDYILIFFLFGIAVTMI
                     AGAGATFEESYNIPTWLGALIMTLAIYITLLLDFNKIVRALGIVTPFLIVLVVLIAGV
                     YLFKGHVSLAEVNQVVPEASIWKGIWFGTIYGGLAFSVGFSTIVAIGGDTEKRTVSGA
                     GAMYGGIIYTVLLALINFALQSEYPTIKNASIPTLTLANNIHPLIATVLSVIMLAVMY
                     NTILGLMYSFAARFTEPYSKNYHIFIIIMMVAGYLLSFVGFAELINKLYTIMGYVGLF
                     IVVAVIIKYFKRKNADKKHIA"
     gene            14037..15080
                     /locus_tag="ACGEXS_001248"
     CDS             14037..15080
                     /locus_tag="ACGEXS_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFF8012266.1"
                     /translation="MNKDNKWTMITALFITVISVLLAFHLKQHYDQITNENHANKDKI
                     NIKNKNVRIYQNLTYNRVFPNSKLDIITPVDMSSNAKLPVIFWMHGGGYIAGDKQYKN
                     PLLAKIAEQGYIVVNVNYALAPQYKYPTPLIQMNQATQFIKENKMNLPIDFNQVIIGG
                     DSAGAQLASQFTAIQTNDRLREAMKFDQSFKPSQIKGAILFGGFYNMQTVRETEFPRI
                     QLFMKSYTGEEDWEKSFKNISQMSTVKQSTKNYPPTFLSVGDSDPFESQNIEFSKKLQ
                     ELNVPVDTLFYDGTHHLHHQYQFHLNKPESIDNIKKVLLFLSRNTSSSGIQTEEKPQI
                     ENPSNELPLNPLN"
BASE COUNT         5100 a         2154 c         2839 g         5163 t
ORIGIN      
        1 gtttcgcagc tttataatgt gcaacttgtc cgtttttagt atgttttatt ttctttttct
       61 aaataaacga ttgattatca tatgaacaat aagtgctaat ccagcgacaa ggcatgtacc
      121 accaatgata gtgaataatg gatgttcttc ccacatactt ttagcaacag tatttgcctt
      181 ttgaataatt ggctgatgaa cttctacagt tggaggtcca taatctttat taataaattc
      241 tcttggatag tccgcgtgta ctttaccatc ttcgactaca agtttataat cttttttact
      301 aaaatcactt ggtaaaacat cgtaaagatc attttcaaca taatatttct taccatttat
      361 cctttgctca cctttagaca atatttttac atatttatac tgatcaaatg agcgttccat
      421 taatgcattc cccatcatat tacgttgctt ctcgccacca aggtttttat agtctcctgc
      481 acccatgata acttgattaa ttctaaattt acctcgtttg gtagtaatcg tatggttgta
      541 atttgctgta tcacttgatc cagtttttaa accatctgta cccggcaaac tcatttttgc
      601 accttccaat gaaaagttga atgtgtaata cgtaactgca tgcgttgttg gtgctaactg
      661 ctttgtaaag tctaatattt taggtgtctc tttaatcacg tgtaaatcta aaatggcata
      721 gtctctagca gtcgttacag tacgttcttg gtctttatac tttgttggtg caaatgtacg
      781 taatcttgaa ttttcagcac ccgttggatt gacgaaatgt gtatttttca ttccgatagc
      841 tttagctttg ttattcatta aatcaacgaa atcgctggtg ttttttgaaa ccttcttagc
      901 taaaattaat gccgcggcat tactagaatt agatactgta atttgtaata ggtctgcgat
      961 tgtccatact tgtccaggat atagtttcgt attactcaac tcaggtagtg tagacataat
     1021 atattctttg ttcgtcattg tgactgtgtc atcaagtgaa agctgcccct tatttacagc
     1081 ttccaatgtt aagtacattg tcattaattt agtcatagac gctggattcc acttagtatc
     1141 gatattgtat tgatacagta attgtccagt ttgacttaca ttaacagcac tcgtcggttc
     1201 gtatgcagcc gacaaacctg cataaccata ttgatttgct gcttgtacag gggttacgtc
     1261 actgttagta gcttgtgcat atggtgtcat aatacttaat gttaaacata aaatgatgat
     1321 aatagatatt aaatttttca taaagcgtta atcttccctt ttccaattct taaatattcc
     1381 ctaaaagcaa tggttattcc tacttacgga aatcattgct aattcacttc accttaatta
     1441 aattgttgaa aataaagttt tctgcagtta atttgaaaaa taatgcaaat atattacgtg
     1501 tgtagctaaa ggtgttataa tgtttgtacg aagagcaaac ttactcaaaa gcgattaatt
     1561 ttcatgtttt aatataaaga ctttgagaag ttattacaaa aaatgcaata gaaatattct
     1621 atcatataaa tgttatgagc ggtattttgg ggcaacactt tatttgattt ttaaagtttt
     1681 gttgggagaa agtatatgat agaaatgcat gtatctatct aaatgaatta actataaatt
     1741 tcaaacagaa gaggtaaaac tatgaaacga gaaaatccat tgtttttctt atttaaaaaa
     1801 ctatcatggc cagtgggtct tatcgttgca gctatcacta tttcatcact agggagctta
     1861 agtggactat tagtgccact gtttactgga cgaattgtag ataaattttc cgtgagccat
     1921 atcaattgga atctaatcgc attatttggt ggtatctttg tcatcaatgc tttattaagc
     1981 ggattaggtt tatatttatt aagtaaaatt ggtgaaaaga ttatttatgc gatacgctca
     2041 gttttatggg agcatatcat acaattaaaa atgccattct ttgacaaaaa tgaaagtggt
     2101 caattaatga gtcgattaac tgacgatacg aaagtgataa atgaatttat ttcacaaaag
     2161 ctacctaact tattaccatc aatcgttaca ttagttgggt cactaatcat gttatttatt
     2221 ttagattgga aaatgacatt attaacattt ataacgatac cgatattcgt tttaattatg
     2281 attcctctag gtcgtattat gcaaaagata tcgacaagta cacaatctga aattgcaaac
     2341 ttcagtggtt tgttagggcg tgtcctaact gaaatgcgtc ttgttaaaat atcaaataca
     2401 gagcgtcttg aattagataa tgcacataaa aatttgaatg aaatatataa attaggttta
     2461 aaacaggcta aaattgcggc agttgtacaa ccaatttcag gtatagttat gttgctaaca
     2521 attgcaatta ttttaggttt tggtgcatta gaaattgcga ctggtgcaat cactgcaggt
     2581 acattaattg caatgatatt ttatgttatt cagttatcta tgcctttaat caatctttcc
     2641 acgttagtta cagattataa aaaggcagtc ggtgcaagta gtagaatata cgaaatcatg
     2701 caagaaccta ttgaaccgac agaagctctt gaagattctg aaaatgtatt aattgatgac
     2761 ggtgtattgt catttgaaca tgtagacttt aaatatgatg tgaagaaaat attagatgat
     2821 gtgtcgttcc aaatcccaca aggtcaagtg agtgcttttg taggcccttc tgggtctggt
     2881 aaaagtacga tatttaatct gatagaacgt atgtatgaaa ttgagtcagg tgatattaaa
     2941 tatggccttg aaagtgtcta tgatatcccg ttatctaagt ggcgacgcaa aattggatat
     3001 gttatgcaat caaattcgat gatgagtggt acaattagag acaatatttt atacggaatt
     3061 aatcgtcatg tttcagatga agaacttatt aattatgcta aattagcgaa ctgtcatgat
     3121 tttatcatgc aatttgatga aggatatgac acgcttgtag gtgaacgagg attgaaactg
     3181 tctggcggac aacgtcaacg tattgatatt gctagaagtt ttgttaaaaa tcctgatatt
     3241 ttgttacttg atgaagcaac agctaatctc gatagtgaaa gtgaattgaa aattcaagaa
     3301 gctttagaaa cattgatgga aggtagaaca acgattgtca ttgcgcatcg tttgtctaca
     3361 attaaaaaag ccggtcaaat tatattctta gacaaaggac aggtaacagg taaaggtacg
     3421 cattcagaac tgatggcatc acatgcgaag tataaaaact ttgtagtgtc tcaaaaatta
     3481 acagattaat tttatatata taagtaagct tggagcaaat acacatatac catcgaggaa
     3541 attaaagtgt ggcacattga tggatataga tgttaataaa ttgcttcaag cttttgtcta
     3601 ttttaaatca tttgagaagt tacgacataa taattcttaa attaatgaaa tcgatatttt
     3661 aagaaaaaaa tgctcatggt ataatacaag ttataagcaa acatacatat attaaatact
     3721 gtagccacga gtcataattc ttcatatttt acatagcaat ttaactgatt ttagagtcca
     3781 cggtacagaa gtttgatatt tcaatgtttc taaattttta aaaaattaaa tcataggtgg
     3841 gtgccaaatg tttttattaa tcaacattat tggtctaatt gtatttcttg gtattgcggt
     3901 attattttca agagatcgca aaaatatcca atggcaatca attgggatct tagttgtttt
     3961 aaacctgttt ttagcatggt tctttattta ttttgattgg ggtcaaaaag cagtaagagg
     4021 agcagccaat ggtatcgctt gggtagttca gtcagcgcat gctggtacag gttttgcatt
     4081 tgcaagtttg acaaatgtta aaatgatgga tatggctgtt gcagccttat tcccaatatt
     4141 attaatagtg ccattatttg atatcttaat gtactttaat attttaccga aaattattgg
     4201 aggtattggt tggttactag ctaaagtaac aagacaacct aaattcgagt cattctttgg
     4261 gatagaaatg atgttcttag gaaatactga agcattagcc gtatcaagtg agcaactaaa
     4321 acgtatgaat gaaatgcgtg tattaacaat cgcaatgatg tcaatgagct ctgtatcggg
     4381 agctattgta ggtgcgtatg tacaaatggt accaggagaa ctggtactaa cggcaattcc
     4441 actaaatatc gttaacgcga ttattgtgtc atgcttgttg aatccagtaa gtgttgaaga
     4501 gaaagaagat attatttaca gtcttaaaaa caatgaagtt gaacgtcaac cattcttctc
     4561 attccttgga gattctgtat tagcagcagg taaattagta ttaatcatca tcgcatttgt
     4621 tattagtttt gtagcgttag ctgatctatt tgatcgtttt atcaatttga ttacaggatt
     4681 gatagcagga tggataggca taaaaggtag tttcggttta aaccaaattt taggtgtgtt
     4741 tatgtatcca tttgcgctat tactcggttt accttatgat gaagcgtggt tggtagcaca
     4801 acaaatggct aagaaaattg ttacaaatga atttgttgtt atgggtgaaa tttctaaaga
     4861 tattgcatct tatacaccac accatcgtgc ggttattaca acattcttaa tttcatttgc
     4921 aaacttctca acgattggta tgattatcgg tacattgaaa ggcattgttg ataaaaagac
     4981 atcagacttt gtatctaaat atgtacctat gatgctatta tcaggtatcc tagtttcatt
     5041 attaacagca gctttcgttg gtttatttgc atggtaatat gtcgaagagt gactatgata
     5101 atacatttta actaataaat atgtccaggc atgtcgtcta ttgatatagg tgagatgctt
     5161 ggactttttt attattgata taaaggtatt taaatatttt taaagttacc gaaattgaag
     5221 cattataaaa accaagtgca catggtaata cacttggctt ttatgggaaa tgaatattat
     5281 tgtacatatg acagtaagga ctaggtacag tcatagtact tcgagcaaaa tttgttttgt
     5341 tattataaac aacacaaagg agataacttc tctattgaag aagttaaaaa cattatagca
     5401 gacaatgaaa tgaaagtaaa ttaaaaattc agaatatttt taattattat attgtgagtg
     5461 atatttatta gggaaagcta ttcttcatat aaattagtta aatagtaaat ctttgttaga
     5521 actctttcca tagagttgtg acagcatttt tgttgtgcta ctatttttat agtctaataa
     5581 atatataaag gggatggttt cgtgaataaa acggttaaag atttaatact agttgtctta
     5641 ggttcattta tctttgctgc aggtgtaaat gcatttatta tttctggtaa cttaggtgaa
     5701 ggcggggtta caggtttagc aattatttta tattatgcgt ttcatatttc accagccatc
     5761 actaacttct tggtcaacgc agtattgatt gccataggtt ataaattttt gagtaagaga
     5821 agtatgtact taactattct tgtaacaatt cttatttcaa tatttttgag tttaacagaa
     5881 tcatggcaag tagaaactgg aaacagcatt gtgaatgcca tttttggtgg tgtaagcgtt
     5941 ggactaggaa tcggagtaat tatccttgca ggcggtacaa cagcaggtac aacaattttg
     6001 gcgagaattg caacgaaata cctcgatgta agcacgccat atgctttgct tttcttcgat
     6061 atgatcgttg ttgcaatttc acttacagtt attccacttg ataaagtatt agtaacagta
     6121 atatcacttt atataggaac aaaagtgatg gaatatgtca tagaaggttt aaacactaaa
     6181 aaagctatga cgattatttc aactaatccc gacaaacttg ccaaagcaat agacgagcaa
     6241 attggaagag gtttaaccat tttaaacgga catggctatt atacgcgtga agaaaaagat
     6301 gtcttatacg ttgttatttc taaaacacaa gtttcaaaag caaagcgatt aattaaacaa
     6361 atcgataaag atgcattcct cgtaattcat gatgtaagag atgtctatgg taatggcttt
     6421 cttgcagatg aataaataaa tggtatgagc acacatactt aaatagaagt ccacggacaa
     6481 gtttttgaac tatgaagact tatctgtggg cgttttttat tttataaaag taatatacaa
     6541 gacatgacaa atcgagctat ccaatttaaa aagtaatgtt agtcaataag attgaaaaat
     6601 gttataatga tgttcatgat aatcattatc aattgggatg tctttgaaaa ttgataattt
     6661 aaaaatagaa attatttttt ataaacagaa agaattttat tgaaagtagg gaaattatga
     6721 atcgtttgca tggacaacaa gttaaaattg gttacgggga taacacgatt ataaataaat
     6781 tagatgttga aataccagat ggcaaagtga cgtcaatcat tggtcctaac ggctgcggga
     6841 aatctacttt gctaaaggca ttgtcacgtt tattggcagt taaagaaggc gaagtatttt
     6901 tagatggtga aaatattcat acacaatcta cgaaagagat tgcaaaaaaa atagccattt
     6961 tacctcaatc acctgaagta gcagatggct taactgttgg ggaattagtt tcatatggtc
     7021 gttttccaca tcaaaaagga tttggtagat taactgctga ggataagaaa gaaattgatt
     7081 gggcaatgga agttacagga actgatacat tccgacaccg ttcaatcaat gatttaagtg
     7141 gtggtcaaag acaacgtgtt tggattgcaa tggcattagc acaaagaact gatattatct
     7201 ttttagacga accaacaaca tatttagata tctgtcatca attagaaata ctagaattag
     7261 ttcagaagct aaatcaggaa caaggttgta caattgtcat ggttcttcat gatatcaacc
     7321 aagcgattcg tttctcagat catcttattg cgatgaaaga aggggatatc atcgctacag
     7381 gttcaacaga agacgtatta acacaggaaa tattagaaaa agtttttaat attgatgttg
     7441 ttttaagtaa agatcctaaa actggaaaac ctttactggt aacttatgac ttatgtcgca
     7501 gagcttattc ttaattaagt aagttaatat gataaaaagg acaattaaca tgacaaatag
     7561 agagaaccca acgccattga agtttttatc ctatattata ggtttaagta tgatactact
     7621 aatcacacta tttatttcta cattaatagg tgacgccaaa attcaagcct ctacaattat
     7681 agaggctatt tttaattata atcctagcaa tcaacagcaa aacatcatca atgagattag
     7741 gattcccaga aatatagcag cagtaattgt aggtatggcg cttgcagttt ctggtgcgat
     7801 tatacaaggt gttactcgta atggtcttgc tgatccggcg ctcataggtt taaattcagg
     7861 tgcttcattt gctttagcat taacatatgc agttttacca aacacttcat ttttaatatt
     7921 gatgtttgct ggatttttag gtgctattct aggaggtgct attgtattaa tgataggccg
     7981 atctagacgt gatggattta atccgatgcg tattatttta gcgggtgcag cagtaagtgc
     8041 tatgttaaca gcgctaagtc aaggtattgc attagctttt agactaaatc aaacagtaac
     8101 attttggact gctggaggcg tttcaggcac aacatggtca caccttaagt gggcaattcc
     8161 attaattggt attgcgttat tcattatatt aacaattagt aaacaactta ccattttaaa
     8221 tcttggtgaa tcattagcta aaggtttagg tcaaaatgta acaatgatca gaggcatatg
     8281 tttaattatt gctatgattc tagcaggtat tgcagttgct atcgctggac aagttgcatt
     8341 tgtaggtttg atggtacctc atatagcaag atttttaatt ggaactgatt atgctaaaat
     8401 tctaccatta acagccttgt taggtgggat actcgtgctt gttgccgatg tgatagcacg
     8461 atatttagga gaagcgcctg ttggtgcaat catttcattt atcggtgttc cttacttttt
     8521 atatttagtt aaaaaaggag gacgctcaat atgattagtt caaataataa acgcagacaa
     8581 ttgatagcac tggctgtttt tagcattcta ctatttctag gttgtacttg gagtattacc
     8641 tcaggtgaat acaacatacc tgttgaaaga tttttcaaaa ctttaattgg acaaggtgat
     8701 gccattgatg agttaatctt attagatttc aggttacctc ggatgatgat tactattttg
     8761 gctggcgcag cgcttagtat tagtggtgca atagtgcaaa gtgtcacaaa aaatccaata
     8821 gctgaaccag gtatattagg tattaacgca ggtggcggat ttgcaatcgc attatttatt
     8881 gcaattggta aaattaatgc tgacaacttt gtttatgtac tgccgttaat aagtatacta
     8941 ggtggtatca ccactgcatt gattattttt attttcagtt ttaataaaaa tgaaggtgtt
     9001 acacctgcga gtatggtatt aataggtgta ggtttacaaa cagcattata tggtggctca
     9061 attacaatta tgtcaaaatt tgatgataag caatctgatt tcatcgctgc ttggtttgca
     9121 ggtaatattt ggggtgacga atggccattt gtcattgcat ttttaccgtg ggtgttgatt
     9181 attattcctt acttactatt taaatcgaat acactaaata ttattcatac gggtgataat
     9241 attgcacgag gtctaggtgt aaggttaagc agagaacgtt taatattatt ctttatcgca
     9301 gtgatgttat catctgctgc tgtagcagta gcaggttcaa tttcgtttat cggattaatg
     9361 ggtccgcata ttgccaaacg tatcgttgga ccacgtcacc agttgttttt accaattgcc
     9421 attttagtag gggcatgttt acttgttata gctgatacaa ttggcaaaat tgtattacaa
     9481 ccaggtgggg ttccagcagg tattgtcgta gcaattattg gtgcaccgta tttcttatat
     9541 ttaatgtaca aaacgaaaaa tgtatagtgt caatggacac aacttattgc tatgaaaggc
     9601 actttattat aaggcttttc atagcatttt ttatttaatg agccactcaa gactatttat
     9661 tttttcaata atgaaccatt aagttatcaa gaggatctta tcaaaaatat atttgataac
     9721 ggtatcaggt taattcttta tgatagcgca ttcatttatt ctgttttata ctatgactga
     9781 taataccaag gaggtacaac atgatgaaaa agttaatcaa taaaaaagaa acatttttaa
     9841 ctgatatgct tgaaggattg ttaattgcgc acccagagtt agatctgatt gctaatacag
     9901 ttattgtaaa aaaagctaag aaagaacatg gtgtagcaat agtctctgga ggtggaagcg
     9961 gacatgaacc tgcgcatgcc ggttttgttg cagaaggtat gctagatgca gcggtttgtg
    10021 gcgaagtatt tacatcacct acacctgata aaatattaga agctattaaa gcagtagata
    10081 ctggtgatgg tgtattacta gttgtaaaaa actatgcagg tgacgtgatg aatttcgaaa
    10141 tggcacaaga gcttgcagaa atggaaggta taaatgttca aactgttatt gttcgtgacg
    10201 acattgctgt gacaaacgaa gtacaacgtc gtggtgttgc aggaacagtg tttgttcata
    10261 agcttgccgg ttatcttgct gaaaaaggtt attcattaac agagataaaa tcgcgtgtag
    10321 aagcgttgtt acctgaaatt aaaagtattg gtatggcaat tgagccaccg cttgttccaa
    10381 ctactggaaa atatggcttt gatattgaag acgacaaaat ggaaatcggt attggtatac
    10441 atggtgaaaa aggtattcat agggaagaag taaaggatat tgatcatatt gttggaacat
    10501 tgttagacga attgtataaa gaagttactg ccaatgatgt catattaatg gtaaatggta
    10561 tgggtggtac gccgttatct gaattaaata tcgtaactaa atatattcaa caaaatttag
    10621 ctgcaagaac ggttaatgtt gctaaatggt ttgttggtga ttatatgaca tctttagaca
    10681 tgcaaggttt ttctataact atcgtgccta ataaaccaga atatttggaa gcatttttag
    10741 caccaacaac aagtcaatac tttaaataag aagtgaatat gaataaatat acatttatga
    10801 ggtggcacaa atgaaagtga atgatatgaa agcacgttta ttaaatttag aagaaacgtt
    10861 taaaaaacat gaatctgaat taactgaatt agatcgagca attggtgatg gtgaccacgg
    10921 ggttaacatg gttcgtgggt ttagtagtct taaagacaaa cttgatgata gctcaatgca
    10981 atcattgttc aaatcaactg gtatggcatt gatgtcaaat gttgggggtg catcaggacc
    11041 actgtatggc tttagctttg ttaaaatgtc tgcagtcacc aaagatgata tggataatca
    11101 agatttcatt acactaattc aggcatttgc cgaagcggtt gaatcacgtg gtaaagttac
    11161 tttaaatgaa aagacaatgt atgatgtagt agcgcgagca gcagagaagc ttaaaaatgg
    11221 tgaaacttta acattcaatg atttacagca attagcagat aatacaaaag atatggtagc
    11281 aacgaaaggt agagctgcat attttggaga agaatcaaaa ggttatattg atccaggtgc
    11341 tcaaagtatg gtttatattt taaacgcttt gattggagat gaagataatg cctaaaatta
    11401 tacttgttag ccacagtaaa gaaattgcaa gtggtacaaa atctttgtta aagcaaatgg
    11461 caggtgacgt tgatattata ccaatcgggg gattaccaga tggttcaatt ggaacttcat
    11521 ttgatatcat ccaagaagtt ttgactaaat tagaggatga tgcattgtgt ttttacgata
    11581 ttggatcttc agaaatgaat gtagatatgg caattgaaat gtatgatggt aatcatcgtg
    11641 tgttaaaagt tgatgcacca attgttgaag gcagttttat cgcagcagta aagctatcaa
    11701 tcggcggttc aattgatgat gcattagcag aaatcaaaca atcattttag ttaaaattta
    11761 ctaataatga aaaatgtaaa cctttttcaa atgaaacttt ataaaaaata tgatagtata
    11821 tatgtaaatg tttaataaaa tctggagaaa taggaggaca ttgccatgca acaccttata
    11881 aaaaaacatg tattgaatgg cgagtttgat ttagtacgac aattgatgtc cgaaacagat
    11941 tttatggaat ttgaagaagc atatatttca agtgcgcatg aagtagaaag tatgatgttt
    12001 tatacatgta ttttagatat gattaagtac gaagaatcat ctgaaatgca tgacttagca
    12061 tttttattgc ttgtgtatcc actaagtgaa tatgaaggtg ctttggattc tgcttattat
    12121 catgcagacg cttccataaa acttactgac ggcaaagaag ttaaaagttt gttacaaatg
    12181 ttattattgc atgcgatacc aacacctgtt atttcagata agaaggcttt tgatatcgcc
    12241 aagcaaattt taaaattaga tcctaataat aatgttgctc gtaacgtctt aaaagacact
    12301 gccaaacgta tggacaacgt tgttgttgat ataaatgaat tacaccaacg taatgcacgt
    12361 taattacatt tcaattatat tagcttaata atagttttaa catttggttg ggttgggcat
    12421 atgttccagc cttttttaat acttaaaaac taacgaagta tacttgtgtg cacaaatggt
    12481 ttttatacaa cattttataa atttatacat tttaataaag aacatacgat agatggttta
    12541 aaccttgtta actgagaaat tttgatatgt attcttcgaa atttaactaa atatacgaaa
    12601 ttcaagaagc acaataatta atcatttttc ctatacaaaa gttcgtatga ctgcattata
    12661 aaagcataaa tttataattt ttttaaatgt cattgaacgt gataatgtga atggattgag
    12721 caattttgaa aaagtgaaaa ataacctatg cgacttgcaa ttaattttca gtacgttata
    12781 atgcacactg tgcaaaatta aggaggtcta ttattcacat gatgatgaat aaagaagcaa
    12841 caaaaattgg atttgcctac gtcggcattg tagtgggcgc aggattttca actggacaag
    12901 aagttatgca atttttcact aaatatggct tgtgggctta tttaggtgtt attatatctg
    12961 gttttatttt agcttttatt gggcgccaag tagcaaaaat tggtactgcc tttgaagcga
    13021 caaatcatga atcaacatta caatacgtat tcggtgaaaa gtttagtaaa gtctttgatt
    13081 atattttaat cttcttctta tttggtatag ctgtaaccat gatagctggt gcaggcgcaa
    13141 catttgaaga aagttataac atacctacat ggctaggtgc tttaattatg acattagcga
    13201 tttatattac gttgttatta gactttaata aaatagtacg tgcactaggt atcgttacac
    13261 catttttaat tgttttagtt gtattaatcg ctggcgttta tttatttaaa ggtcatgttt
    13321 cattagcaga agttaaccaa gtagtgcctg aagcaagtat ttggaaggga atctggtttg
    13381 gtacaatata tggtggatta gctttttctg taggttttag taccatcgta gcaatcggtg
    13441 gggatactga aaagcgtaca gtgtcaggtg caggcgcgat gtatggtggt attatctata
    13501 ctgtattact agcattgatc aactttgcat tgcaaagtga atatccaact attaaaaatg
    13561 cctcaattcc tacattgacg ttagcaaata atatccatcc tttaatagca acagtgttat
    13621 ctgttattat gctggcggtt atgtataata ctattctagg actaatgtat tcatttgcag
    13681 cacgttttac agaaccatac agtaaaaatt atcatatctt tattattata atgatggtag
    13741 caggttattt attaagtttc gtaggatttg ctgaattaat taataagtta tatacaatta
    13801 tgggatatgt aggcttattt attgtagtag ctgtaattat taaatatttc aaacgtaaaa
    13861 atgcggataa aaaacatatt gcttaatatc atatgaggga tatccgaaac tttacaattg
    13921 aatcactttg ttttaacctt aaaagcaatt cgtctctact cttatcgggc gaattgcttt
    13981 ttatatttat tcagtctatt aatatgagcg tctaacaaat agagaggtac gatgtaatga
    14041 ataaagataa taaatggacg atgataactg cgctttttat aactgtaatc agtgtattgt
    14101 tagcatttca tctgaaacaa cattatgacc aaattacaaa tgagaaccat gctaataaag
    14161 acaaaattaa tattaaaaat aaaaatgtgc gcatttatca aaaccttaca tacaatagag
    14221 ttttccctaa cagtaaatta gatattatta cacctgttga tatgtcttct aatgccaaac
    14281 tgccagttat tttttggatg cacggtggtg gttatattgc gggtgataag cagtataaaa
    14341 acccattatt agcgaaaatt gctgaacaag ggtacattgt tgtgaatgta aattatgcat
    14401 tggcgccaca atataaatat cccacaccat taattcaaat gaatcaagca actcaattca
    14461 ttaaagaaaa taaaatgaat ttacctattg attttaatca agtaattatt ggcggtgatt
    14521 ctgcaggtgc tcaattagct agccaattta cggcaataca gacgaatgat cgcttaagag
    14581 aagccatgaa atttgatcag tcattcaaac catcgcaaat taaaggtgct atactatttg
    14641 gtggttttta taatatgcaa acagttagag aaactgagtt tccaagaata cagttattta
    14701 tgaaaagtta tactggcgaa gaagattggg aaaagagttt taaaaacatt tcacaaatgt
    14761 cgacagtaaa acaatcgaca aaaaattatc caccaacatt tttatctgtt ggagatagcg
    14821 atccattcga aagtcaaaat atagaattca gtaagaaatt acaagaattg aatgtaccag
    14881 tagatacttt gttttatgat ggtacgcatc atttacatca tcagtatcaa tttcacctta
    14941 ataaacctga atcgatagat aatatcaaaa aagtgttact tttcttaagt cgtaatacat
    15001 cctctagtgg tattcaaact gaagagaaac cacaaataga aaatccgagt aatgaattac
    15061 cgttaaatcc tttaaactaa tgataaacag tagtaattta ttacttaagc aacatttaag
    15121 attttcaaat taaaaacgaa gaatttaaaa catgtggtgc taatgtgtaa gagtctgaga
    15181 tataataaat tgttagcggt tctttatcat ttctatctca ctctattatg atgtgacatt
    15241 tattttacaa actaat
//
LOCUS       DAXWFK010000052        14754 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.052, whole genome shotgun sequence.
ACCESSION   DAXWFK010000052 DAXWFK010000000
VERSION     DAXWFK010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14754
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.052"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(202..1200)
                     /gene="sirC"
                     /locus_tag="ACGEXS_001249"
     CDS             complement(202..1200)
                     /gene="sirC"
                     /locus_tag="ACGEXS_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirC"
                     /protein_id="HFF8012267.1"
                     /translation="MTEKINKKDNYHLIFALIFLAIVSVVSMMIGSSFIPLQRVLMYF
                     INPNDSMDQFTLEVLRLPRITLAILAGAALGMSGLMLQNVLKNPIASPDIIGITGGAS
                     LSAVVFIAFFSHLTIHLLPLFAVLGGAVAMMILLVFQTKGQIRPTTLIIIGISMQTLF
                     IALVQGLLITTKQLSAAKAYTWLVGSLYGATFKDTIILGMVILAVVPLLFLVIPKMKI
                     SILDDPVAIGLGLHVQRMKLIQLITSTILVSMAISLVGNIGFVGLIAPHIAKTIVRGS
                     YAKKLLMSAMIGAISIVIADLIGRTLFLPKEVPAGVFIAAFGAPFFIYLLLTVKKL"
     gene            complement(1197..2192)
                     /gene="sirB"
                     /locus_tag="ACGEXS_001250"
     CDS             complement(1197..2192)
                     /gene="sirB"
                     /locus_tag="ACGEXS_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirB"
                     /protein_id="HFF8012268.1"
                     /translation="MLLKPKYQIVIAGLCLAIVAILSLMIGNTLVSPGTVIQALFNFD
                     SENDLHDVVTGARASRTIIALLTGAALAVSGLLMQALTRNPIASPGLFGVNAGAVFFV
                     IFSITFIQIQSFKMIVVIAFLGAIVVTVLVVALGMFRQTLFSPHRVILAGAAIAMLFT
                     AFTQGILIMNETDLQGLLFWLSGSVSLRNIWDIPWIIPLVLILILIAFSMAAHINILM
                     TSDDIATGLGQNIKLIKWMIIMLISMLAGISVAVAGSIVFVGLIVPNISKRLLPPNYK
                     YLIPFTALAGAILMIISDIVARIIIKPLELPIGVVTAVIGAIVLIYIMKKGRQRL"
     gene            complement(2208..3200)
                     /gene="sirA"
                     /locus_tag="ACGEXS_001251"
     CDS             complement(2208..3200)
                     /gene="sirA"
                     /locus_tag="ACGEXS_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter
                     substrate-binding protein SirA"
                     /protein_id="HFF8012269.1"
                     /translation="MNKVIKMLVVTLAFLLVLAGCSGNSNKQSSDNKDKETTSIKHAM
                     GTTEIKGKPKRVVTLYQGATDVAVSLGVKPVGAVESWTQKPKFEYIKNDLKDTKIVGQ
                     EPAPNLEEISKLKPDLIVASKVRNEKVYDQLSKIAPTVSTDTVFKFKDTTKLMGKALG
                     KEKEAEDLLKKYDDKVAAFQKDAKAKYKDAWPLKASVVNFRADHTRIYAGGYAGEILN
                     DLGFKRNKDLQKQVDNGKDIIQLTSKESIPLMNADHIFVVKSDPNAKDAALVKKTESE
                     WTSSKEWKNLDAVKNNQVSDDLDEITWNLAGGYKSSLKLIDDLYEKLNIEKQSK"
     gene            3431..4411
                     /gene="sbnA"
                     /locus_tag="ACGEXS_001252"
     CDS             3431..4411
                     /gene="sbnA"
                     /locus_tag="ACGEXS_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diaminopropionate biosynthesis protein SbnA"
                     /protein_id="HFF8012270.1"
                     /translation="MIEKSQACHDSLLDSVGQTPMVQLHQLFPKHEVFAKLEYMNPGG
                     SMKDRPAKYIIEHGIKHGLITENTHLIESTSGNLGIALAMIAKIKGLKLTCVVDPKIS
                     PTNLKIIKSYGANVEMVEEPDAHGGYLMTRIAKVQELLATIDDAYWINQYANELNWQS
                     HYHGAGTEIVETIKQPIDYFVAPVSTTGSIMGMSRKIKEVHPNAQIVAVDAKGSVIFG
                     DKPINRELPGIGASRVPEILNRSEINQVIHVDDYQSALGCRKLIDYEGIFAGGSTGSI
                     IAAIEQLITSIEEGATIVTILPDRGDRYLDLVYSDTWLEKMKSRQGVKSE"
     gene            4408..5418
                     /gene="sbnB"
                     /locus_tag="ACGEXS_001253"
     CDS             4408..5418
                     /gene="sbnB"
                     /locus_tag="ACGEXS_001253"
                     /EC_number="1.5.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-[(2S)-2-amino-2-carboxyethyl]-L-glutamate
                     dehydrogenase SbnB"
                     /protein_id="HFF8012271.1"
                     /translation="MNREMLYLNRSDIEQAGGNHSQVYVDALTEALTAHAHNDFVQPL
                     KPYLRQDPENGHIADRIIAMPSHIGGEHAISGIKWIGSKHDNPSKRNMERASGVIILN
                     DPETNYPIAVMEASLISSMRTAAVSVIAAKHLAKKGFKDLTIIGCGLIGDKQLQSMLE
                     QFDHIERVFVYDQFSEACARFVDRWQQQRPEINFIATENAKEAVSNGEVVITCTVTDQ
                     PYIEYDWLQKGAFISNISIMDVHKEVFIKADKVVVDDWSQCNREKKTINQLVLEGKFS
                     KEALHAELGQLVTGDIPGREDDDEIILLNPMGMAIEDISSAYFIYQQAQQQNIGTTLN
                     LY"
     gene            5439..7193
                     /gene="sbnC"
                     /locus_tag="ACGEXS_001254"
     CDS             5439..7193
                     /gene="sbnC"
                     /locus_tag="ACGEXS_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis protein SbnC"
                     /protein_id="HFF8012272.1"
                     /translation="MQNHTAVNTAQAIILRDLVDALLFEDIAGIVSNSEITKENGQTL
                     LIYERETQQIKIPVYFSALNMFRYESSQPITIEGRVSKQPLTAAEFWQTIANMNCDLS
                     HEWEVARVEEGLTTAATQLAKQLSELDLASHPFVMSEQFASLKDRPFHPLAKEKRGLR
                     EADYQVYQAELNQSFPLMVAAVKKTHMIHGDTANIDELENLTVPIKEQATDMLNDQGL
                     SIDDYVLFPVHPWQYQHILPNVFAKEISEKLVVLLPLKFGDYLSSSSMRSLIDIGAPY
                     NHVKVPFAMQSLGALRLTPTRYMKNGEQAEQLLRQLIEKDEALAKYVMVCDETAWWSY
                     MGQDNDIFKDQLGHLTVQLRKYPEVLAKNDTQQLVSMAALAANDRTLYQMICGKDNIS
                     KNDVMTLFEDIAQVFLKVTLSFMQYGALPELHGQNILLSFEDGRVQKCVLRDHDTVRI
                     YKPWLTAHQLSLPKYVVREDTPNTLINEDLETFFAYFQTLAVSVNLYAIIDAIQDLFG
                     VSEHELMSLLKQILKNEVATISWVTTDQLAVRHILFDKQTWPFKQILLPLLYQRDSGG
                     GSMPSGLTTVPNPMVTYD"
     gene            7186..8442
                     /gene="sbnD"
                     /locus_tag="ACGEXS_001255"
     CDS             7186..8442
                     /gene="sbnD"
                     /locus_tag="ACGEXS_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B export MFS transporter"
                     /protein_id="HFF8012273.1"
                     /translation="MINQSIWRSNFRILWLSQFIAIAGLTVLVPLLPIYMASLQNLSV
                     VEIQLWSGIAIAAPAVTTMIASPIWGKLGDKISRKWMVLRALLGLAVCLFLMALCTTP
                     LQFVLVRLLQGLFGGVVDASSAFASAEAPAEDRGKVLGRLQSSVSAGSLVGPLIGGVT
                     ASILGFSALLMSIAVITFIVCIFGALKLIETTHMPKSQTPNINKGIRRSFQCLLCTQQ
                     TCRFIIVGVLANFAMYGMLTALSPLASSVNHTAIDDRSVIGFLQSAFWTASILSAPLW
                     GRFNDKSYVKSVYIFATIACGCSAILQGLATNIEFLMAARILQGLTYSALIQSVMFVV
                     VNACHQQLKGTFVGTTNSMLVVGQIIGSLSGAAITSYTTPATTFIVMGVVFAVSSLFL
                     ICSTITNQINDHTLMKLWELKQKSAK"
     gene            8432..10168
                     /gene="sbnE"
                     /locus_tag="ACGEXS_001256"
     CDS             8432..10168
                     /gene="sbnE"
                     /locus_tag="ACGEXS_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2,3-diaminopropanoate--citrate ligase SbnE"
                     /protein_id="HFF8012274.1"
                     /translation="MQNKELIQHAAYAAIERILNEYFREENLYQVPPQNHQWSIQLSE
                     LETLTGEFRYWSAMGHHMYHPEVWLIDGKSKKITTYKEAIARILQHMAQSADNQTAVQ
                     QHMAQIMSDIDNSIHRTARYLQSNTIDYVEDRYIVSEQSLYLGHPFHPTPKSASGFSE
                     ADLEKYAPECHTSFQLHYLAVHQDVLLTRYVEGKEDQVEKVLYQLADIDISEIPKDFI
                     LLPTHPYQINVLRQHPQYMQYSEQGLIKDLGVSGDSVYPTSSVRTVFSKALNIYLKLP
                     IHVKITNFIRTNDLEQIERTIDAAQVIASVKDEVETPHFKLMFEEGYRALLPNPLGQT
                     VEPEMDLLTNSAMIVREGIPNYHADKDIHVLASLFETMPDSPMSKLSQVIEQSGLAPE
                     AWLECYLNRTLLPILKLFSNTGISLEAHVQNTLIELKDGIPDVCFVRDLEGICLSRTI
                     ATEKQLVPNVVAASSPVVYAHDEAWHRLKYYVVVNHLGHLVSTIGKATRNEVVLWQLV
                     AHRLMTWKKEYANNAVFVDCVEDLYQTPTIAAKANLMSKLNDCGANPIYTHIPNPICH
                     NKEVSYCESNNS"
     gene            10077..11927
                     /gene="sbnF"
                     /locus_tag="ACGEXS_001257"
     CDS             10077..11927
                     /gene="sbnF"
                     /locus_tag="ACGEXS_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(L-alanin-3-ylcarbamoyl)-2-[(2-
                     aminoethylcarbamoyl)methyl]-2-hydroxypropanoate synthase
                     SbnF"
                     /protein_id="HFF8012275.1"
                     /translation="MIVVQTLFIHIYQIQFVITRRYRIVNQTILNRVKTRVMHQLVSS
                     LIYENIVVYKASYQDGVGHFTIEGHDSEYRFTAEKTHSFDRIRITSPIERVVGDEADT
                     TTDYTQLLREVVFTFPKNDEKLEQFIVELLQTELKDTQSMQYRESNPPATPETFNDYE
                     FYAMEGHQYHPSYKSRLGFTLSDNLKFGPDFVPNVKLQWLAIDKDKVETTVSRNVVVN
                     EMLRQQVGDKTYEHFVQQIEASGKHVNDVEMIPVHPWQFEHVIQVDLAEERLNGTVLW
                     LGESDELYHPQQSIRTMSPIDTTKYYLKVPISITNTSTKRVLAPHTIENAAQITDWLK
                     QIQQQDMYLKDELKTVFLGEVLGQSYLNTQLSPYKQTQVYGALGVIWRENIYHMLIDE
                     EDAIPFNALYASDKDGVPFIENWIKQYGSEAWTKQFLAVAIRPMIHMLYYHGIAFESH
                     AQNMMLIHENGWPTRIALKDFHDGVRFKREHLSEAASHLTLKPMPEAHKKVNSNSFIE
                     TDDERLVRDFLHDAFFFINIAEIILFIEKQYGIDEELQWQWVKGIIEAYQEAFPELNN
                     YQHFDLFEPTIQVEKLTTRRLLSDSELRIHHVTNPLGVGGINDATTISET"
     gene            11902..12678
                     /gene="sbnG"
                     /locus_tag="ACGEXS_001258"
     CDS             11902..12678
                     /gene="sbnG"
                     /locus_tag="ACGEXS_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis citrate synthase
                     SbnG"
                     /protein_id="HFF8012276.1"
                     /translation="MQQLSLKHRLNNGDSVYGIFNSIPDPLMIEVIAASGYDFVVIDT
                     EHVAINDETLAHLIRAAEAAHIIPIVRVTAVIDRDIIKVLDMGARGIIVPHVKDRETV
                     EHIVKLSRYYPQGLRSLNGGRMARFGRTPLLDAMEMANEHIMVIAMIEDVEGVMAIDD
                     IAQVEGLDMIVEGAADLSQSLGIPWQTRDDQVTSHVQHIFEVVNAHGKHFCALPREDE
                     DIAKWQAQGVQTFILGDDRGKIYRHLSASLATSKQKGDEG"
     gene            12678..13880
                     /gene="sbnH"
                     /locus_tag="ACGEXS_001259"
     CDS             12678..13880
                     /gene="sbnH"
                     /locus_tag="ACGEXS_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis decarboxylase
                     SbnH"
                     /protein_id="HFF8012277.1"
                     /translation="MRIVQPVIEQLKAQSHPVCHYIYDLVGLEHHLQHITSSLPSNCQ
                     MYYAMKANSERKILDTISQYVEGFEVASQGEIAKGLAFKPANHIIFGGPGKTDEELRY
                     AVSEGVQRIHVESMHELQRLNAILEDEDKTQHILLRVNLAGPFPNATLHMAGRPTQFG
                     ISEDEVDDVIEAALAMPKIHLDGFHFHSISNNLDSNLHVDVVKLYFKKAKAWSEKHRF
                     PLKHINLGGGIGVNYADLTNQFEWDNFVERFKTLIVEQEMEDVTLNFECGRFIVAHIG
                     YYVTEVLDIKKVHGAWYAILRGGTQQFRLPVSWQHNHPFDIYRYKDNPYSFEKVSISR
                     QDTTLVGQLCTPKDVFAREVQIDAISTGDVIVFKYAGAYGWSISHHDFLSHPHPEFIY
                     LTQTKEDE"
     gene            13884..14648
                     /gene="sbnI"
                     /locus_tag="ACGEXS_001260"
     CDS             13884..14648
                     /gene="sbnI"
                     /locus_tag="ACGEXS_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transcriptional
                     regulator/O-phospho-L-serine synthase SbnI"
                     /protein_id="HFF8012278.1"
                     /translation="MNHIHEHLKLVPVDKIDLHETFEPLRLEKTKSSIEADDFIRHPI
                     LVTAMQHGRYMVIDGVHRYTSLKALGCKKVPVQEIHETQYSISTWQHKVPFGVWWETL
                     QQEHRLPWTTETRQEAPFITMCHGDTEQYLYTKDLGEAHFQVWEKVVASYSGCCSVER
                     IAQGTYPCLSQQDVLMKYQPLSYKEIEAVVHKGETVPAGVTRFNISGRCLNLQVPLAL
                     LKQDDDVEQLRNWKQFLADKFANMRCYTEKVYLVEQ"
BASE COUNT         4901 a         2424 c         2956 g         4473 t
ORIGIN      
        1 tttgaaaaat cgaagaatat tgattgatat tatagaatgt gagtcggtgt tatttgtata
       61 taaagtatta atatgtaatt aagaaagtcg acgcttgtta acaataacaa caactatatg
      121 tataaaaaaa cctcgcaacg gttagttaac tgaatcattt agctaacttc gttgtgaggt
      181 cattttgttt taataatatc gttataactt tttcacggtt aataataagt atatgaagaa
      241 tggggcacca aaagcagcaa taaatacacc tgctggcact tctttaggca agaataaggt
      301 acgcccaatt aagtctgcaa taacaattga tatggcacca atcattgctg acattagtaa
      361 ctttttagca taacttccgc gaacgattgt tttcgcgata tgtggtgcga ttaaaccgac
      421 aaacccaatg ttacctacta aactgattgc catagatacg agtatagtag aagtgattaa
      481 ttggattagt ttcatacgtt gtacatgtaa gcctaagcca atcgctacag ggtcatcaag
      541 tatagatatt ttcatttttg gtataacaag aaataacaac ggcacaacag ctaaaataac
      601 catacccaaa atgattgtat ctttaaacgt agcaccgtaa agacttccga ctagccatgt
      661 ataagctttg gcagcagata attgcttcgt tgtaatgagt aatccttgga caagcgcaat
      721 aaacaacgtt tgcatcgaaa taccgatgat tatgagtgtt gtcgggcgta tttgtccttt
      781 cgtttgaaac actaatagta tcatcattgc aactgcgcca cctaatactg caaatagtgg
      841 aagtaaatgt attgttaaat ggctgaaaaa tgcaataaag acaacagcac ttaagctagc
      901 accacctgtg ataccgataa tatcaggtga ggcaattgga ttttttaata cattttgcaa
      961 cattaaacca ctcattccta gtgcggcacc tgctaaaatc gcaagtgtaa tgcgaggtaa
     1021 gcgtaatact tctaaagtga attgatccat actgtcattt ggatttataa agtacatcag
     1081 tacgcgttgt aatggtataa agcttgaacc aatcatcata cttaccactg aaacgatggc
     1141 taaaaagatt aacgcgaaga tgagatggta attgtctttt ttattaatct tttcggtcat
     1201 aagcgttgac gtcctttctt cataatatag attaagacaa tagcgccaat gacagcggta
     1261 acgacaccga taggcaactc tagtggctta attattatac gagcaacaat gtctgaaatg
     1321 atcattagga ttgctccagc taatgcagta aaaggaatta aatacttata gtttggtggt
     1381 aataatcgtt tgctaatatt cggtacgata agacccacaa agacgattga tccagctacg
     1441 gctaccgaaa taccggctaa catactgatg agcataataa tcatccattt gattaatttt
     1501 atgttttgac cgaggccggt tgcaatgtcg tcacttgtca tcaagatgtt gatgtgtgca
     1561 gccatgctaa atgcaattaa aataagtatc aatacaagcg gaataatcca tgggatatcc
     1621 caaatattac gtaatgaaac ggagccactt aaccaaaata ataggccttg taagtctgtt
     1681 tcgttcataa taagtatgcc ttgagtaaag gctgtaaata gcatcgcaat cgcagcacct
     1741 gccaaaatga cacggtgagg tgagaatagt gtttgtctaa acatacctag tgcaacaact
     1801 aatacagtaa caacaatagc ccccaaaaat gcaataacta caatcatttt aaaagattga
     1861 atttggataa atgtaatact aaaaatgaca aaaaatactg cgcctgcatt gacaccgaaa
     1921 agccctggtg aggctattgg gtttcgtgta agtgcttgca tcaacaaacc tgagacagca
     1981 agggcagcac cagtcaataa cgcaatgatt gttctcgacg cccgtgcacc agtgacaaca
     2041 tcatgtaaat cgttttcact atcaaagttg aataacgcct gtatcaccgt acctggtgac
     2101 acaagcgtat ttccaatcat taaacttaag atagctacta ttgcaagaca taaaccagca
     2161 ataacgattt ggtattttgg tttaagtagc atcgtaaaac tccttaatta ttttgattgt
     2221 ttttcaatat ttaacttttc atataaatcg tcaataagtt ttaatgaaga tttatatccg
     2281 ccagctaagt tccaagtgat ttcatctaaa tcatcagata cttggttgtt tttaactgcg
     2341 tctaaatttt tccactcttt acttgaagtc cattcgcttt cagtcttttt aactaatgca
     2401 gcatctttcg catttggatc tgattttact acaaaaatat gatcagcgtt cattaatgga
     2461 atgctttctt tagatgtaag ttggataata tctttaccat tatcaacttg tttttgtaag
     2521 tctttattac gtttgaatcc taaatcattt aagatttcac cagcatatcc accagcataa
     2581 attcttgtat gatcagcacg gaagttaaca actgaagctt tcaatggcca tgcatcttta
     2641 tactttgctt ttgcatcttt ttggaatgca gctactttat catcgtactt tttaagtaaa
     2701 tcttcagctt ctttttcttt ccctaaagct ttccccatta acttagttgt atctttgaat
     2761 ttgaaaactg tatcagtaga aactgttggt gcgattttag ataattgatc gtaaactttt
     2821 tcatttctaa cttttgacgc gacaattaag tccggtttta atttagagat ttcctctaag
     2881 ttaggtgcag gttcttgacc tacaatctta gtatctttta aatcattttt tatgtattcg
     2941 aatttcggtt tttgtgtcca tgattctaca gcacctacag gtttaacacc taaagataca
     3001 gcgacgtcag tggcaccttg atatagcgta acaacacgct ttggtttccc tttaatttca
     3061 gttgtaccca ttgcatgttt aattgaagtt gtttccttat ctttgttatc agatgattgt
     3121 ttatttgaat tcccactaca tcctgctaaa acaagtagga aagcaagcgt aacaacaagc
     3181 attttaatta ctttattcat tgactaatta gcctccttcg tgatgtatga caatgagaat
     3241 cattatcacg atttagtatg aattaaattt tttcctaagt caataaaata tttatgattt
     3301 acatgcaact tataattatt tgacatataa atgcataaaa aatataatcc taattacttg
     3361 atagtgagaa tcattatcaa ttaggtaaca cacaatatta tagaatttta aatttgagga
     3421 ggaagcgctt ttgattgaaa aaagtcaagc atgtcacgat tcattgttag attctgtagg
     3481 gcaaacacct atggttcaac ttcatcaact atttccgaaa catgaagtgt ttgcaaagtt
     3541 agagtatatg aatcctggag gcagcatgaa agatcgacct gccaagtaca tcattgaaca
     3601 tggtattaaa catggtttaa tcactgagaa tacacattta attgaaagta cttctggtaa
     3661 tttaggcatt gcgttggcaa tgatagctaa aatcaaggga ttaaaactca cgtgtgttgt
     3721 tgatcctaaa atatcaccaa caaatttgaa aattattaaa agttatggtg ccaatgtaga
     3781 aatggttgaa gaacctgatg cacatggggg ttatttaatg actcgtattg caaaggtgca
     3841 agaactgtta gccactattg acgatgcata ttggattaat caatatgcga atgagttaaa
     3901 ttggcaatcc cattatcatg gtgcaggcac agagattgtt gaaacaatta agcaacctat
     3961 agattatttt gtcgcgccag tcagcacgac aggtagcatt atgggtatga gtagaaaaat
     4021 aaaagaagtg catccaaacg cacaaattgt tgctgttgat gcgaaagggt cagtcatttt
     4081 tggtgacaaa cctattaata gagaattacc tggtatcggt gctagtcgtg tacccgaaat
     4141 attgaataga tcagaaatta atcaagtgat ccatgtagat gattatcaat ctgctttggg
     4201 ctgtcgaaaa ctgattgatt atgaaggcat atttgccgga ggttcaacag gttcgattat
     4261 tgcagcgatt gagcagttga taacgtcaat tgaagaaggt gcaacaattg tcacgatttt
     4321 accagatcga ggcgatcgtt acttagattt agtttattca gatacatggt tagaaaaaat
     4381 gaaatcaaga caaggagtta aatcagaatg aatagagaga tgttgtattt aaatagatca
     4441 gatattgaac aagcgggagg taatcattca caagtttatg tggacgcatt aacagaagca
     4501 ttaacagccc atgcgcacaa tgattttgta caaccgctta agccgtattt aagacaggat
     4561 cctgaaaatg gacacatcgc agatcgaatt attgcaatgc caagtcatat cggtggtgaa
     4621 cacgcaattt caggtattaa gtggataggt agtaagcacg acaatccatc gaaacgtaat
     4681 atggagcgtg caagtggcgt cattattttg aatgatccag aaacgaatta tccaattgca
     4741 gttatggaag caagtttaat tagtagtatg cgtactgcag cagtttcagt gattgcagca
     4801 aagcatttgg ctaaaaaagg atttaaagac ttaacaatca ttggatgcgg gctaatcgga
     4861 gacaagcaat tacaaagtat gttagagcaa ttcgatcata ttgaacgcgt gtttgtttac
     4921 gatcaattct ctgaagcatg tgcacgcttt gttgatagat ggcaacaaca gcgtccggaa
     4981 attaatttta ttgcgacaga aaatgctaaa gaagcagtat caaatggtga agtagtcatt
     5041 acatgtaccg taacggatca accatacatt gaatatgatt ggttacaaaa gggtgcattt
     5101 attagcaaca tttctatcat ggatgtgcat aaagaagtct ttattaaagc tgacaaagtc
     5161 gtagtagatg actggtcaca atgtaatcga gaaaagaaaa ctattaacca attggtgtta
     5221 gaaggtaaat tcagcaaaga agctcttcat gctgaactag gacaacttgt gacaggtgac
     5281 ataccaggac gtgaagacga tgatgagatc atattactta atccgatggg tatggctatc
     5341 gaagatattt caagtgctta ttttatttat caacaggcac aacaacaaaa tattgggaca
     5401 acattgaacc tatattaaga atgcgaggtg tctgaacatt gcagaatcat acagcagtca
     5461 atacagcaca agcgataata ttaagagatt tagttgatgc attattattt gaagatatag
     5521 ccggaattgt atcgaatagt gagattacta aagaaaacgg acaaacgctt ttgatatacg
     5581 aacgtgaaac acaacaaata aagatacctg tttattttag tgctttaaat atgtttcgtt
     5641 acgaaagttc acaaccaatt acgatagagg gaagggtgtc taagcaacct ttaacggcag
     5701 ctgaattttg gcaaacaatt gctaatatga attgtgatct aagtcatgaa tgggaagtgg
     5761 ctcgcgttga agaaggactg actactgctg ccacacagct tgctaaacaa ttatcagaat
     5821 tagatttagc gtcacatcct tttgtgatgt cagagcagtt tgcaagttta aaagatcgtc
     5881 catttcatcc attagctaaa gaaaaaagag gattaagaga agcggattat caagtgtatc
     5941 aagctgaatt aaatcaatca tttcctttaa tggttgcagc agttaaaaag acacatatga
     6001 ttcatggcga tactgcaaat atcgatgaat tagaaaattt gacagtacct ataaaagaac
     6061 aagcgacaga catgttaaat gatcaagggt tatcaataga tgactatgta ctatttccgg
     6121 tacatccttg gcaatatcag catattctgc cgaacgtctt tgcgaaagag attagtgaaa
     6181 agttggttgt actattaccg ttaaaatttg gagattatct gtcgtcttca agtatgcgtt
     6241 cattaattga tattggcgca ccgtataacc atgtcaaagt accatttgca atgcagtcat
     6301 taggcgcatt aaggctaacg cctacgcgtt acatgaaaaa cggagaacaa gcagaacaat
     6361 tattacgtca gcttatagaa aaagatgaag cactagctaa gtatgtcatg gtttgtgatg
     6421 aaacagcttg gtggtcatat atgggtcaag ataatgatat tttcaaagat caattaggtc
     6481 atctaactgt tcagctaaga aagtatcccg aagtgctagc caaaaatgat acgcaacagc
     6541 tagtgtcaat ggcagcactc gcggcaaatg atcgcacttt atatcaaatg atttgtggaa
     6601 aagataatat ttctaaaaat gatgtcatga cgttatttga agatatcgcg caagtctttt
     6661 taaaggtaac actatcattt atgcaatacg gcgcattacc agagttgcat ggtcaaaata
     6721 tattgttgtc atttgaagat ggacgtgtac aaaaatgcgt gttacgtgat catgatactg
     6781 tcagaattta taaaccatgg ctaacagcac atcagctttc attgccgaag tatgtcgtca
     6841 gagaagatac acctaatacg ctaattaatg aggatttgga aacattcttt gcttattttc
     6901 aaacattagc tgtatcggta aatctatatg ccattattga tgcaattcaa gatttatttg
     6961 gtgtaagtga gcatgaactt atgtcgttgt taaaacaaat tttaaaaaat gaagtggcaa
     7021 ctatttcctg ggttacaact gatcagctag ctgtcagaca cattttattt gataaacaga
     7081 cgtggccatt caaacaaatt ttattaccat tgctatatca acgtgatagt ggtggaggta
     7141 gtatgccttc aggtttaact accgtaccaa atccaatggt gacatatgat taatcagtct
     7201 atatggcgca gtaactttcg cattttatgg ctcagtcagt ttatagcgat tgctggactg
     7261 acagtacttg tgccattatt gccaatttat atggcatcac tacaaaatct atcagtcgta
     7321 gaaatacagt tgtggagtgg tatagcgatt gctgctccag ctgtaacgac gatgatagct
     7381 tcgccgatat gggggaagct aggtgataag atcagccgaa aatggatggt gttaagagcg
     7441 ttacttggtt tggcggtatg cttattttta atggcattgt gtacgacacc attacagttt
     7501 gtacttgtga ggttattgca gggactattt ggtggtgttg ttgatgcatc aagtgcgttt
     7561 gcgagtgcag aggcgccagc tgaagatcgt ggaaaggtat taggaagact gcaaagttca
     7621 gtcagcgcag ggtctcttgt ggggccatta attggcggtg ttacagcttc gatattaggt
     7681 tttagtgcgt tactgatgag tattgccgtt attactttta ttgtctgtat tttcggtgca
     7741 ttaaaattga ttgaaacgac acatatgcca aaatcacaaa caccaaatat taataaaggt
     7801 attcgccgtt catttcaatg tctattatgc acacaacaaa catgtcgatt tattatcgtt
     7861 ggcgttttag caaactttgc tatgtatggc atgctaactg cattatcacc acttgcttca
     7921 tcagtgaatc atacagcgat agatgaccgt agtgtgattg gatttttaca gtccgcattt
     7981 tggacggctt cgatattaag cgcgccttta tggggacgct ttaatgataa atcatatgtt
     8041 aaatcagtat atatatttgc cacgattgca tgtggttgta gtgcgatact gcaaggttta
     8101 gcgacgaata tagagttttt aatggctgca agaatacttc aaggattaac atatagtgca
     8161 ttgattcaaa gtgtcatgtt tgttgtcgtg aatgcgtgtc atcaacaact taaaggcaca
     8221 tttgttggaa cgacgaacag tatgttagtt gttggtcaaa ttattggcag tcttagtggc
     8281 gctgccatta caagttatac tacaccagct actacgttta tcgttatggg cgtagtattt
     8341 gcagtaagta gtttattttt aatttgttca accatcacta atcaaatcaa cgatcacaca
     8401 ttaatgaaat tatgggagtt gaaacaaaaa agtgcaaaat aaagaattaa tacaacatgc
     8461 agcgtatgcg gctatcgaac gcattttaaa tgaatatttt agagaagaaa atttatatca
     8521 agtaccacct caaaatcatc aatggtctat acaattatca gagctcgaaa ctttaacggg
     8581 tgaatttcgc tattggtctg cgatggggca tcatatgtat catccagagg tatggcttat
     8641 cgatggaaaa agtaaaaaaa taacaactta taaagaagca attgcgcgta ttttgcaaca
     8701 tatggctcaa agtgcagata atcaaacggc agtgcaacaa catatggcgc aaattatgtc
     8761 tgacatcgat aatagcattc atcgcacggc acgttatttg caaagtaaca caatagacta
     8821 cgtagaggat cgttatatcg tttcagaaca atctttatac ttaggtcatc catttcatcc
     8881 gactcctaag agtgcaagtg ggttttcaga agcagattta gagaaatatg cacccgaatg
     8941 tcatacatca ttccaattgc attatttagc tgtgcatcaa gatgttctgc tcacgcgcta
     9001 tgtagaaggt aaagaagatc aggttgagaa agtgttgtat caattagcag acatagatat
     9061 atcagagata cccaaagatt ttattttatt accaacacat ccttatcaaa tcaatgtgtt
     9121 gcgacagcat ccacagtata tgcaatatag tgaacaaggt ttaataaaag accttggcgt
     9181 ttccggtgat tcagtgtacc cgacgtcttc ggttagaact gtattttcaa aagcattaaa
     9241 catttattta aaattaccga tacacgttaa aatcactaat tttatacgta cgaatgacct
     9301 tgaacagatt gaacggacaa ttgatgccgc gcaagttatc gcatcagtca aagatgaggt
     9361 tgaaacaccc cattttaaat tgatgtttga agaaggatat cgtgcattgt taccgaatcc
     9421 attagggcaa acagttgaac ctgaaatgga tttattaaca aatagtgcca tgattgttcg
     9481 tgaagggata ccgaattacc atgctgataa agatattcat gtattggcgt cattatttga
     9541 aacgatgcct gattcaccga tgtctaagtt atcacaagtg attgagcaaa gtggtttagc
     9601 accagaagca tggcttgaat gttatttgaa tcgtacatta ttgccgatat taaagctgtt
     9661 tagtaacaca ggcattagtc tagaagcaca tgtacaaaat acattaattg aattaaaaga
     9721 tggcataccc gacgtatgct ttgtcagaga tcttgaaggc atttgtctat ctagaacgat
     9781 tgctactgaa aaacagcttg tgccaaatgt tgtggcagca tcaagccctg ttgtatatgc
     9841 acatgatgaa gcatggcatc gtcttaaata ttacgttgta gtaaatcact taggacattt
     9901 agtatcaact attggtaaag cgactagaaa tgaagttgtg ttatggcaac ttgtagcgca
     9961 tcgtcttatg acttggaaaa aagaatacgc gaataacgca gtatttgttg actgtgtaga
    10021 agatttatat caaacgccga ccattgcggc taaagcgaat ttgatgagta aattgaatga
    10081 ttgtggtgca aaccctattt atacacatat accaaatcca atttgtcata acaaggaggt
    10141 atcgtattgt gaatcaaaca attcttaatc gtgtaaagac tagagtgatg caccaactgg
    10201 tatcatcact tatttatgag aatattgttg tgtataaagc gtcatatcaa gacggtgtcg
    10261 gtcattttac aatagaagga catgattcag agtatcgttt tactgctgaa aagacacata
    10321 gctttgatcg tatacgtatc acatcaccaa ttgagcgtgt cgtaggagat gaggcagata
    10381 caacaacaga ctatacacaa ttattgagag aggttgtatt tacatttcct aaaaatgatg
    10441 aaaagctaga acaatttatt gtcgagttat tacagacaga attaaaagat acgcaaagta
    10501 tgcagtatcg agaatcaaac ccaccagcaa cacctgagac atttaacgac tatgaatttt
    10561 atgcgatgga agggcatcag tatcatccaa gttacaaatc acgtttagga tttacgttga
    10621 gtgataattt gaaatttggt cctgattttg taccaaacgt taaactgcag tggttagcta
    10681 tcgacaaaga taaagtagaa acgacggtat caagaaatgt tgtagttaac gaaatgttac
    10741 gtcaacaagt tggcgataag acttatgaac attttgtaca gcaaattgaa gcatctggca
    10801 aacatgtaaa tgatgttgag atgatacctg tacacccatg gcagtttgaa catgtcatcc
    10861 aagttgattt ggctgaagaa aggcttaatg gcacagtact atggttaggg gaaagtgatg
    10921 agctatatca ccctcaacaa tcgattcgta cgatgtcgcc aatagacacg acaaaatatt
    10981 atttaaaggt accaataagt ataacgaaca cttcaacgaa acgagtgttg gcgcctcata
    11041 caattgaaaa tgcagcgcaa attacggatt ggttaaagca gatacagcaa caagatatgt
    11101 atttaaaaga tgaattaaag acagtttttc taggggaagt cttaggacag tcttatttaa
    11161 atacacaact ttcgccttat aaacaaactc aagtttatgg tgcgttaggt gttatatggc
    11221 gtgaaaatat atatcatatg ttaatcgatg aagaggatgc gataccattt aatgcacttt
    11281 atgcaagtga taaggatggt gtaccattca ttgaaaattg gattaaacaa tatggttctg
    11341 aagcttggac aaagcaattt ttagctgtag cgattcgtcc aatgattcat atgctttatt
    11401 atcacggtat tgcctttgaa tcgcatgcac aaaatatgat gctcattcat gaaaatggtt
    11461 ggcctacacg tattgcctta aaagatttcc atgatggtgt tcgttttaag cgtgagcatt
    11521 taagtgaagc tgcttcacac ctgacattaa agccaatgcc agaagcacat aaaaaagtga
    11581 atagtaattc atttattgaa acagatgacg aacgtttagt acgcgacttt ttacatgatg
    11641 catttttctt tattaatatc gccgaaatca tcttatttat tgaaaagcaa tatggtatcg
    11701 atgaggagct gcaatggcaa tgggttaaag gcatcatcga ggcgtatcaa gaagcatttc
    11761 cagagttgaa taactatcaa catttcgatt tgtttgaacc tacgattcaa gttgaaaagt
    11821 taacgacacg tcgattatta agtgactccg agttaagaat tcatcatgtt acaaatccat
    11881 taggtgtagg aggtatcaat gatgcaacaa ctatctctga aacatagatt aaacaatggt
    11941 gattcagttt atggcatttt taattctata ccggacccat tgatgatcga ggttatcgca
    12001 gcaagcgggt atgactttgt tgtgattgat acagaacacg tggcgattaa tgatgagaca
    12061 ctagcgcatt taattcgtgc agctgaagca gcgcatatta taccaattgt acgtgtcact
    12121 gcagtgatag atagagatat cattaaagtg ttggatatgg gtgcgagagg tattattgtg
    12181 ccacacgtta aagatcgtga gacagttgag catattgtga aattaagtcg ttattacccg
    12241 caaggattaa gaagtttgaa tggtggtcgc atggcaagat ttggacgtac accattactt
    12301 gatgcaatgg agatggctaa tgagcatatt atggtgattg ccatgataga agatgttgaa
    12361 ggggttatgg ccattgacga tatagcacaa gtcgaaggtt tagacatgat agtcgaaggt
    12421 gccgcagatt tatcgcagtc acttggcata ccatggcaaa cgcgtgatga tcaagtaaca
    12481 tcacatgttc aacatatttt tgaagttgtg aatgcacatg gtaaacattt ttgtgcatta
    12541 ccacgtgaag atgaagatat tgcaaaatgg caggcacaag gtgtacaaac atttatttta
    12601 ggtgatgatc gcggaaaaat atatcgccat ttaagtgcat ctctagcgac gtctaaacag
    12661 aaaggggatg aaggctaatg cgtatagttc aacctgttat tgaacaatta aaagcacaat
    12721 ctcatccagt ttgtcattat atctatgatt tagtcggact ggaacatcat ttgcaacata
    12781 ttacatcgtc attgccgagt aattgtcaaa tgtactatgc aatgaaagca aatagtgaac
    12841 gaaaaatcct agatacaatt agtcagtatg ttgaaggatt cgaagttgca tctcaaggtg
    12901 aaatagcaaa aggtcttgct tttaaaccag caaatcatat tatttttggt ggccctggta
    12961 agacagacga ggaactaaga tatgcagtaa gtgaaggtgt tcagcgtatt catgttgaaa
    13021 gtatgcatga attacaacgg ctaaatgcca tcttagaaga tgaagataag acacaacaca
    13081 ttttattgcg tgttaattta gcaggaccat ttcccaatgc aacgttgcat atggcaggac
    13141 gcccaacaca atttggtatt tctgaagacg aagttgatga tgtcattgaa gctgcgctcg
    13201 caatgccaaa gattcatcta gatggatttc attttcattc tatttctaac aatttagact
    13261 cgaatttaca tgtcgatgta gtgaaacttt attttaaaaa agcaaaggca tggtctgaaa
    13321 aacatcgatt tccactcaaa catatcaatc ttggtggtgg cataggcgtt aactatgcag
    13381 atttaactaa ccaatttgaa tgggataatt ttgtagaacg ttttaaaaca cttatcgttg
    13441 agcaagaaat ggaagatgtg acattgaact ttgaatgtgg gcgctttatt gtggcacata
    13501 ttggttacta tgtgacagaa gtgctagaca ttaagaaagt acatggtgct tggtatgcca
    13561 tattaagagg aggtacgcaa caatttagac tgccggtatc ttggcaacat aaccatcctt
    13621 ttgacattta tcgctataag gacaatccat attcatttga aaaagtttca atttcgagac
    13681 aggacacaac gttagtcggt caattatgta caccgaaaga tgtctttgct agagaagtac
    13741 agatagacgc aatcagtaca ggcgacgtta ttgttttcaa atatgcaggt gcatacggat
    13801 ggtctatttc acatcacgat ttcttaagcc atccacatcc tgaatttatt tatttaacgc
    13861 aaacaaagga ggatgaataa ctattgaatc atattcatga acatttaaaa ttggtaccag
    13921 tagataagat tgatcttcac gaaacattcg aacctttaag attggaaaaa acgaaaagta
    13981 gtattgaagc agatgatttt atacgtcatc ctattttagt gacagcgatg caacatggta
    14041 gatatatggt tatagatggt gtgcatcggt atacaagttt gaaagcgtta ggatgtaaga
    14101 aagttccagt gcaagaaatc catgaaacac aatattcaat tagtacatgg caacataaag
    14161 ttccatttgg tgtgtggtgg gaaacgttac aacaagaaca tcgcttgcca tggactactg
    14221 agacaagaca agaagcgcca tttattacaa tgtgtcatgg tgatacagaa caatatttgt
    14281 atacaaaaga tttaggcgaa gcacattttc aagtatggga aaaggttgtc gcaagttata
    14341 gtggttgttg ttctgtagag agaattgcac aaggtacata tccttgtctt tctcaacaag
    14401 atgtactcat gaagtatcag ccattgagtt ataaggaaat tgaagcggtt gttcataaag
    14461 gggaaactgt gccagcaggt gtgacacgct ttaatatttc aggacgatgt cttaatcttc
    14521 aagtaccact ggcattactt aaacaagatg atgatgttga acaactgcgc aattggaagc
    14581 agtttttagc agataagttt gccaatatga gatgctatac tgaaaaagta tacttggtgg
    14641 agcaatagtt ttactgtgat gttgagggaa atatgatgat ttagcgtatt gatagcgaaa
    14701 atataataaa acaatatagt gtggagaact tttgatattt tataaatatt gaag
//
LOCUS       DAXWFK010000053        14629 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.053, whole genome shotgun sequence.
ACCESSION   DAXWFK010000053 DAXWFK010000000
VERSION     DAXWFK010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14629
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.053"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..940
                     /locus_tag="ACGEXS_001261"
     CDS             <1..940
                     /locus_tag="ACGEXS_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HFF8012279.1"
                     /translation="DWIFIVFIISVYYIFWLLAKRKYILVTFWTIIILTLLITFPTNS
                     HHKITMLNVGQGDSILYEGGKNQNVLIDTGGKVIDDTKQPSYSISKYHILPTLNERGI
                     NELEYLILTHPHNDHIGEVEYIISHIKIKHIVIYNKGYSSNTLMLLSKLSHKYNIKLM
                     DVRQVSSFKLGDSSFLFFDSFIPNSRDKNEYSIITMITYQNKKVLLMGDASKNNESLL
                     LKKYNLPEIDILKVGHHGSKTSSSKEFIEMIKPKISLISSGKNNMYHLPNIEVVKRLQ
                     RIRSRIYNSQQNGQVTIDLDDNLKVDSSSYGNASGL"
     gene            997..1971
                     /gene="holA"
                     /locus_tag="ACGEXS_001262"
     CDS             997..1971
                     /gene="holA"
                     /locus_tag="ACGEXS_001262"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFF8012280.1"
                     /translation="MSDNIVAIYGDVPELVEKQSAEIISQFLKSDRDDFNFVKYNLYE
                     TEIAPIVEETLTLPFFSDKKAILVKNAYIFTGEKAPKDMAHNVDQLIEFIEKYDGENL
                     IVFEIYQNKLDERKKLTKTLKKHARLKKIEQMSEEEIKKWIQSKLNENFKDIKRDALD
                     LFIELTGINFNIVSQEIEKLILFLGDRPTINKQDVNQIINRSLEQNVFLLTEYIQKRK
                     KEQAIHLVKDLITMKEEPIKLLALITSNYRLFYQCKILSQKGYSGQQIAKTIGVHPYR
                     VKLALGQVRHYQLDELLNIIDACAETDYKLKSSYMDKQLILELFILSL"
     gene            complement(2016..2267)
                     /gene="rpsT"
                     /locus_tag="ACGEXS_001263"
     CDS             complement(2016..2267)
                     /gene="rpsT"
                     /locus_tag="ACGEXS_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFF8012281.1"
                     /translation="MANIKSAIKRVKTTEKAEARNISQKSAMRTAVKNAKTAVSNNAD
                     NKNELVSLAVKLVDKAAQSNLIHSNKADRIKSQLMTANK"
     gene            2613..4436
                     /gene="lepA"
                     /locus_tag="ACGEXS_001264"
     CDS             2613..4436
                     /gene="lepA"
                     /locus_tag="ACGEXS_001264"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFF8012282.1"
                     /translation="MDNEQRLKRRENIRNFSIIAHIDHGKSTLADRILENTKSVETRD
                     MQDQLLDSMDLERERGITIKLNAVRLKYEAKDGNTYTFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNELELLPVINKIDLPAAEPERVKQEIEDMI
                     GLDQDDVVLASAKSNIGIEEILEKIVEVVPAPDGDPEAPLKALIFDSEYDPYRGVISS
                     IRIVDGVVKAGDKIRMMATGKEFEVTEVGINTPKQLPVDELTVGDVGYIIASIKNVDD
                     SRVGDTITLASRPASEPLQGYKKMNPMVYCGLFPIDNKNYNDLREALEKLQLNDASLE
                     FEPESSQALGFGYRTGFLGMLHMEIIQERIEREFGIELIATAPSVIYQCVLRDGSEVT
                     VDNPAQMPDRDKIDKIFEPYVRATMMVPNDYVGAVMELCQRKRGQFINMDYLDDIRVN
                     IVYELPLAEVVFDFFDQLKSNTKGYASFDYEFIENKESNLVKMDILLNGDKVDALSFI
                     VHRDFAYERGKALVEKLKTLIPRQQFEVPVQAAIGQKIVARTNIKSMGKNVLAKCYGG
                     DISRKRKLLEKQKAGKAKMKAVGNVEIPQDAFLAVLKMDDE"
     gene            4999..6123
                     /gene="hemW"
                     /locus_tag="ACGEXS_001265"
     CDS             4999..6123
                     /gene="hemW"
                     /locus_tag="ACGEXS_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFF8012283.1"
                     /translation="MTVQSAYIHIPFCVRICTYCDFNKYFIQNQPVDEYLDALITEMS
                     TAKYRILKTMYVGGGTPTALSINQLERLLKAIRDTFTITGEYTFEANPDELTKEKVQL
                     LEKYGVKRISMGVQTFKPELLSVLGRTHNTEDIYTSVLNAKNAGIKSISLDLMYHLPK
                     QTIEDFEQSLDLALDMDIQHISSYGLILEPKTQFYNMYRKGLLKLPNEDLGADMYQLL
                     MSKIEQSPFHQYEISNFALDGHESEHNKVYWFNEEYYGFGAGASGYVDGVRYTNINPV
                     NHYIKAINKESKAILVSNKPSLTERMEEEMFLGLRLNEGVSSSRFKKKFDQSIESVFG
                     QTINNLKEKELIVEKNDVIALTNRGKVIGNEVFEAFLIND"
     gene            6224..7201
                     /gene="hrcA"
                     /locus_tag="ACGEXS_001266"
     CDS             6224..7201
                     /gene="hrcA"
                     /locus_tag="ACGEXS_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFF8012284.1"
                     /translation="MITDRQLSILNAIVEDYVDFGQPVGSKTLIERHNLNVSPATIRN
                     EMKQLEDLNYIEKTHSSSGRSPSQLGFRYYVNRLLEQTSHQKTNKLRRLNQLLVENQY
                     DVSSALTYFADELSNISQYTTLVVHPNHKQDIINNVHLIRANPNLVIMVIVFSSGHVE
                     HVHLASDIPFNNDKLNTISNFVTNKLTEFNQNLQDDIVSFVQSEQEEIFINKLLNTMN
                     NHISNQSNSIYMGGKVKLIDALNESNVSSIQPILQYIESNRIAELLQDISSPNINVKI
                     GNEIDDSLSDISIVTSQYHFDETLKGQIAVIGPTAMHYQNVIQLLNRIW"
     gene            7233..7859
                     /gene="grpE"
                     /locus_tag="ACGEXS_001267"
     CDS             7233..7859
                     /gene="grpE"
                     /locus_tag="ACGEXS_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFF8012285.1"
                     /translation="MTNKDESVEKNTESTVEETNVKQNIDDSVEQAEESKGHLQDEAI
                     EETSDENVIEEIDPKDQKNNELQQLADENEEKYLRLYAEFENYKRRIQKENEINKTYQ
                     AQRVLTDILPAIDNIERALQIEGDDETFKSLQKGVQMVHESLINALKDNGLEVIKTEG
                     EAFDPNIHQAVVQDDNPDFESGEITQELQKGYKLKDRVLRPSMVKVNQ"
     gene            7928..9760
                     /gene="dnaK"
                     /locus_tag="ACGEXS_001268"
     CDS             7928..9760
                     /gene="dnaK"
                     /locus_tag="ACGEXS_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFF8012286.1"
                     /translation="MSKIIGIDLGTTNSCVTVLEGDEPKVIQNPEGSRTTPSVVAFKN
                     GETQVGEVAKRQAITNPNTVQSIKRHMGTDYKVDIEGKSYTPQEISAMILQNLKNTAE
                     SYLGEKVDKAVITVPAYFNDAERQATKDAGKIAGLEVERIINEPTAAALAYGLDKTDK
                     DEKVLVFDLGGGTFDVSILELGDGVFEVLSTAGDNKLGGDDFDQVIIDYLVAEFKKEN
                     GVDLSQDKMALQRLKDAAEKAKKDLSGVSQTQISLPFISAGENGPLHLEVNLTRSKFE
                     ELSDSLIRRTMEPTRQAMKDAGLTNSDIDEVILVGGSTRIPAVQEAVKKEIGKEPNKG
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIEILGGRMNTLIERNTTIPTSK
                     SQIYSTAVDNQPSVDVHVLQGERPMAADNKTLGRFQLTDIPPAERGKPQIEVTFDIDK
                     NGIVNVTAKDLGTNKEQRITIQSSSSLSDEEIDRMVKDAEVNAEADKKRREEVDLRNE
                     ADSLVFQVEKTLTDLGENIGEEDKKSAEEKKDALKTALEGQDIEDIKSKKEELEKVIQ
                     ELSAKVYEQAAQQQQQAQGANAGQNNDSTVEDAEFKEVKDDDKK"
     gene            9896..11035
                     /gene="dnaJ"
                     /locus_tag="ACGEXS_001269"
     CDS             9896..11035
                     /gene="dnaJ"
                     /locus_tag="ACGEXS_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031072 - heat shock protein binding
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFF8012287.1"
                     /translation="MAKRDYYEVLGISKDASKDEIKKAYRKLSKKYHPDINKEEGADE
                     KFKEISEAYEVLSDDNKRASYDQFGHDGPQGFGGQGFNGSDFGGFSGFGGGGFEDIFS
                     SFFGGGRQRDPNAPQKGDDLQYTMTLTFEEAVFGTTKEISIRKDVTCETCHGDGAKPG
                     TSKKTCSYCNGAGHVAVEQNTILGRVRTEQVCPKCNGSGQEFEEACPTCHGKGTENKT
                     VKLEVKVPEGVDNEQQIRLAGEGSPGVNGGPAGDLYVVFRVKPSETFKRDGDDIYYKL
                     NVSFPQAALGDEIKIPTLNNEVMLTIPAGTQTGKQFRLKEKGIKNVHGYGYGDLYVDI
                     KVVTPTKLTDRQKELMKEFAQLNGEEINEQPSNFKDRAKRFFKGE"
     gene            11039..11977
                     /gene="prmA"
                     /locus_tag="ACGEXS_001270"
     CDS             11039..11977
                     /gene="prmA"
                     /locus_tag="ACGEXS_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFF8012288.1"
                     /translation="MNWTELSIIINHEAVELATNILENHGSNGVVIEDSDDLINQPED
                     KYGEIYALKKEDYPDKGVRLKAYFNEMTYDDKLRQQIKDELLNLDELDQHNVQFSEQI
                     IAETDWENEWKNYFHPFRASKKFTIVPSWETYAKEADEELCIELDPGMAFGTGDHPTT
                     SMCLKAIETYVLPQHSVIDVGTGSGILSIASHLIGVKRIKALDIDEMAVSVAKENFRR
                     NHCETLIEAVPGNLLKDETEKFDIVIANILAHIIDEMIEDAYNTLNEGGYFITSGIIK
                     EKYEGIQSHMERVGFKIISEQHDNGWVCLVGQKVSE"
     gene            11979..12731
                     /locus_tag="ACGEXS_001271"
     CDS             11979..12731
                     /locus_tag="ACGEXS_001271"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFF8012289.1"
                     /translation="MQRYFIDQNADVSQRFFITKKEDIHHITNVMRYDVGSKIILTFN
                     DQTVYLCEIADITSDKIEVDLKEKQNINTELPVDVTICSGLIKADKYEWLLQKATELG
                     ASSFIAVSMERSIVKLNEAKVAKKIERWQKIIKEAAEQSYRLVIPSIQFESNLKLICD
                     TIDNYDYILIAYEEEAKDGELSNFKQTLQQFNAQDKVLMIFGPEGGLSENEISLFSNT
                     STVVGLGPRILRAETAPLYALSAISYEKELMG"
     gene            12738..14084
                     /gene="mtaB"
                     /locus_tag="ACGEXS_001272"
     CDS             12738..14084
                     /gene="mtaB"
                     /locus_tag="ACGEXS_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0061712 - tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HFF8012290.1"
                     /translation="MSTVAFHTLGCKVNHYETEAIWQLFKEANYERVDFEANADVFVI
                     NTCTVTNTGDKKSRQIIRRAIRQNPDAVICVTGCYAQTSSAEIMEIPGVDVVVGTQDR
                     HKLLGYIDEFRKERQPINGVGNIMKNRKYEELDVPYFTDRTRASLKIQEGCNNFCTFC
                     IIPWARGLMRSRDPEKVVEQATQLVNSGYKEIVLTGIHTGGYGQDLKDYNLAQLLRDL
                     ETINGLERIRISSIEASQLTDEVIDVLERSTKVVRHLHIPLQSGSDTVLKRMRRKYTM
                     DRFSERLTKLHKALPDLAVTSDVIVGFPGETEAEFQETYDFIVKHKFSELHVFPYSPR
                     IGTPAARMDDQIDEEIKNERVHKLITLSNQLGKLYASKFDQDVLEVIPEEQGDTEGTL
                     VGYADNYMKVQFEGDESLIGQIVKVKITQANYPLNEGQAIKVVDFATNKSDREVLV"
     gene            14377..14553
                     /gene="rpsU"
                     /locus_tag="ACGEXS_001273"
     CDS             14377..14553
                     /gene="rpsU"
                     /locus_tag="ACGEXS_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFF8012291.1"
                     /translation="MSKTVVRKNESLEDALRRFKRSVSKSGTIQEVRKREFYEKPSVK
                     RKKKSEAARKRKFK"
BASE COUNT         5540 a         1951 c         2764 g         4374 t
ORIGIN      
        1 tgattggata tttatagtat ttataatttc tgtttattac atattttggt tattggctaa
       61 acgtaaatat atattggtta cgttttggac tataattatt ctgacattat taataacgtt
      121 tccaacaaat tcacatcaca aaattacaat gttaaatgtg gggcagggag acagtatttt
      181 atatgaaggc ggtaagaacc aaaatgtctt gattgataca ggtgggaaag tgattgatga
      241 tactaaacaa cctagttatt caatttctaa atatcatatt ttaccaacgc taaatgaaag
      301 agggataaat gaattagagt atctaatttt aacacatcca cacaatgacc atattggtga
      361 agtggaatat attattagtc atattaaaat taaacatata gtgatataca ataagggata
      421 tagtagtaat acattgatgt tattatcgaa attaagccat aagtacaaca ttaaacttat
      481 ggatgtaaga caagttagta gttttaaact tggagatagt agttttctat tttttgatag
      541 ttttattcca aatagccgag ataaaaatga gtattcgatt attactatga ttacatatca
      601 aaataaaaaa gttttattaa tgggcgatgc tagtaaaaat aatgaatctt tactactaaa
      661 aaaatataac ttgccggaga ttgatatttt aaaagtagga catcatggga gcaagacaag
      721 tagttctaaa gaatttatag agatgattaa gcctaaaata agtttgattt cttctgggaa
      781 gaacaatatg tatcatcttc ctaatataga agttgttaaa cgattgcaaa ggattcgcag
      841 tcgcatttac aatagtcaac aaaacggtca agttacaatt gacttagatg ataatttaaa
      901 agttgattca agctcttatg gaaatgcaag tggtttatag atacagaaac atgatataat
      961 ctattgatgt gtcaaacgaa tgaaagggtg taatgaatga gcgacaatat tgtagctatt
     1021 tatggagatg tgcctgaatt ggttgaaaaa caaagtgcag aaatcatatc acaatttttg
     1081 aaaagtgata gagatgactt taactttgtg aaatataatt tatacgaaac agagattgca
     1141 ccaattgttg aagaaacatt aacattgcct ttcttttcag ataaaaaagc aattttggtt
     1201 aaaaatgcat atatatttac aggtgaaaaa gcgccaaaag atatggctca taatgtagat
     1261 caattaatag aatttattga aaaatatgat ggcgaaaatt tgattgtctt tgagatatat
     1321 caaaataaac ttgatgaaag aaaaaagtta actaaaactc taaaaaagca tgcaaggctt
     1381 aaaaaaatag agcaaatgtc tgaagaagaa ataaaaaaat ggattcaaag taaattaaat
     1441 gagaatttca aagatatcaa aagagatgca ttagatttat ttattgagtt gacaggtatt
     1501 aactttaata ttgtctcaca agagatagaa aagttgattt tatttttagg cgatagacca
     1561 acaattaata agcaggatgt taaccaaatt attaatagaa gtttagagca aaatgtattt
     1621 ttactgactg aatacattca gaaaagaaag aaagaacaag caattcattt agtaaaagat
     1681 ttaataacta tgaaagaaga accaattaaa ttacttgcac taattacaag taattaccga
     1741 ttattttatc aatgtaagat tctgagtcaa aaaggatata gtggacagca aattgctaaa
     1801 acaataggag tacatccata cagagtaaaa ttagcgttag gacaagtaag acattatcaa
     1861 cttgatgaat tattgaatat tatagatgct tgtgcggaaa ctgattataa acttaaatca
     1921 tcatatatgg ataaacagtt aatactggaa ttattcattc tatctttata aaaaataaaa
     1981 gttcaagcat gcgcttgaac ttttatttaa aaagattatt tatttgcagt cattaattgt
     2041 gatttaatac ggtcagcttt gtttgaatgt attaaattac tttgagcagc tttgtctact
     2101 aacttaactg ctaagcttac taattcattt ttattatcag cgttatttga aacagctgtt
     2161 ttagcgtttt taactgctgt acgcattgca ctcttttgtg aaatgttgcg tgcttcagct
     2221 ttttcagttg tttttacacg tttaattgca gatttgatat ttgccatttc tgtcacctcc
     2281 taaaagtgag ttcatcttat caaaatttta tttgattaca acaagaaata ttttaacaaa
     2341 agtctaaccg ttgtgcaatc atttatttga tggggttagc ttaaaatttt gcagatttaa
     2401 taaattgtgt tggaaacaca atcaatacgt tttataatta taagtacgta attaacatat
     2461 taacatatcg caagtatgta tttaaataaa gattgttata atttcaaagt tcatccaaga
     2521 ttatggcgtt tgcatttacc tattaaaaac gttattatat caaagatgcg aaagataata
     2581 cgggtttatt ttatgaaagt gagaaggata aaatggataa tgagcaacgc ttaaaaagaa
     2641 gagagaatat aaggaatttc tcgattatag cacatattga ccacggaaaa tctacattgg
     2701 ctgatagaat tttagaaaat accaaatcag ttgaaacaag agatatgcaa gatcagttac
     2761 tagattcaat ggatttagaa agagaacgtg gtattacaat caaattaaac gcagtacgtt
     2821 taaagtacga agctaaagat ggaaatactt atacattcca tttaatcgat acgcctggac
     2881 acgtcgattt tacatatgaa gtgtcacgtt ctttggcagc ttgtgagggc gcgattttag
     2941 tagtagatgc ggctcaaggt atcgaagcac aaacattagc aaatgtttat ttagcattag
     3001 ataatgagtt agagttattg cctgttatta acaaaattga tttacctgct gcagaacctg
     3061 aacgcgtgaa acaagaaatt gaagatatga taggtttaga ccaagacgat gttgttttag
     3121 caagtgctaa atctaacatt ggaattgaag agatactaga gaaaatagtt gaagttgtgc
     3181 cagctccaga tggtgaccca gaagcaccac taaaagcgtt aatatttgat tctgagtatg
     3241 atccatatag aggggtaatt tcatcgataa gaattgtgga cggtgttgtt aaagccggag
     3301 ataaaattcg aatgatggcc actggtaaag agttcgaagt aacagaagtt ggaattaata
     3361 cacctaagca gcttccagtt gatgaattaa cagttggtga tgttggttat attattgcaa
     3421 gtattaaaaa tgttgatgat tctagggttg gtgacaccat cacattagct agtagacctg
     3481 catcagaacc attgcaaggt tataagaaaa tgaatccaat ggtatattgc ggactgttcc
     3541 caatagataa caaaaattat aatgatttaa gagaagcatt agaaaaatta caattgaatg
     3601 atgcatcatt agaatttgag cctgaatcgt cacaagcatt aggttttggt tatagaactg
     3661 gtttcttagg tatgttacac atggaaataa ttcaagaaag aattgaaaga gaatttggta
     3721 ttgaattaat tgcaactgca ccatctgtaa tttatcaatg tgttttaagg gacggttcag
     3781 aagtgacggt tgataaccca gcacaaatgc cagatcgtga taaaattgat aaaatatttg
     3841 agccatatgt tcgtgcaact atgatggttc caaatgacta tgtcggtgca gtaatggaat
     3901 tatgtcaacg taaacgtgga caatttataa atatggacta tttagatgat attcgtgtaa
     3961 atattgttta tgaattacct ttagctgaag ttgtatttga tttcttcgat caacttaaat
     4021 ctaatactaa aggatatgca tcatttgatt atgaattcat cgaaaataaa gaaagtaatt
     4081 tagtcaagat ggatatttta ttaaatggtg ataaagtgga tgcgctaagc ttcatagttc
     4141 atagagattt tgcatatgaa cgtggtaaag cattagttga aaaacttaaa acgttaattc
     4201 caagacagca atttgaagta cctgtacagg ctgcaatagg acaaaaaatt gtagcgcgta
     4261 caaatattaa atcaatgggt aaaaacgttt tagctaaatg ttatggcggt gacataagcc
     4321 gtaaacgtaa attacttgaa aaacaaaaag caggtaaagc taagatgaaa gcagttggta
     4381 atgttgaaat tccacaagat gctttcttgg ctgtattgaa aatggatgat gaataatttt
     4441 aaaaaatcaa ttaacaattt acaatgaata aagtttaata actaaaaaga gggagcctag
     4501 gataaattaa cgtcctgggc tttacaatgt tatattggca gccatcgaca gagttaaaat
     4561 gagcttataa caatggggcc ccaacacaga agctgacgaa aagtcagctt actataatgt
     4621 gcaagttggg gtggggcccc aacatagaga atttcgaaaa gaaattctac aggcaatgca
     4681 agttggggtg ggacgacgaa ataaattttg cgaaaatatc atttctgtcc cactccctta
     4741 tgcatgagtt ttactcatgt aattttattt ttaaggacat attacatctg gctaatgtgt
     4801 aagagccact acataataaa tcattagtgg ttctttatta tttctatctc actccctcta
     4861 aacaagaata aatattaaaa tgaatcgata tattagacaa tcattgatta aacgttaaag
     4921 ttaaaagtaa gaataattgc agatagtcca acaggatata gccgattgga taaaaagtct
     4981 gagaagcgga gcattaaaat gacggtacaa agtgcatata tacatattcc attttgtgta
     5041 agaatatgta catattgtga tttcaataaa tattttatac agaatcaacc tgtagatgag
     5101 tacttagatg cactaatcac agaaatgtct acagcaaaat ataggatctt aaagaccatg
     5161 tatgtgggtg gcggcacacc aacggccctt tctattaatc agttggaaag attacttaaa
     5221 gcaatacgtg atacgtttac aatcacaggc gagtatacat ttgaagcaaa tcctgatgag
     5281 ttaactaaag agaaagtcca actattagag aaatatggag taaaaaggat ttcaatgggc
     5341 gttcaaacat tcaagccgga gttattgtct gttttaggta gaacgcacaa tactgaagat
     5401 atttacactt cggtgttaaa tgctaaaaac gcaggtatta aatcaatcag tttagattta
     5461 atgtatcatt taccgaaaca gacgattgaa gattttgaac aaagtttaga tctagcttta
     5521 gatatggata ttcaacatat ttcgagttac ggcttaatac ttgaacctaa aacccaattt
     5581 tataatatgt atagaaaagg cttgctcaaa cttcctaatg aggatttagg tgctgacatg
     5641 tatcagttgc tgatgtctaa gatagaacaa tctcctttcc atcaatacga aatatctaat
     5701 tttgcattag atggccatga atcagaacat aataaggttt actggtttaa tgaggaatat
     5761 tatggatttg gagcaggtgc aagtggttat gtagatggtg tgcgttatac gaatatcaat
     5821 ccagtgaatc attatatcaa agctataaat aaagaaagta aagcaatttt agtatcaaat
     5881 aaaccttctt tgactgagag aatggaagaa gaaatgtttc ttgggttgcg tttaaatgaa
     5941 ggtgtgagta gtagtaggtt caaaaagaag tttgaccaat ctattgaaag tgtctttggt
     6001 caaacaataa ataatttaaa agagaaggaa ttaattgtag aaaagaacga tgtgattgca
     6061 cttacaaata gagggaaagt cataggtaat gaggtttttg aagctttcct aataaatgat
     6121 taaaaaaaat tgaaatttcg agtctttaac attgacttac tttgaccaat ttgataaatt
     6181 ataattagca cttgagataa gtgagtgcta atgaggtgaa aacatgatta cagataggca
     6241 attgagtata ttaaacgcaa ttgttgagga ttatgttgat tttggacaac ccgttggttc
     6301 taaaacacta attgagcgac ataacttgaa tgttagtcct gctacaatta gaaatgagat
     6361 gaaacagctt gaagatttaa actatatcga gaagacacat agttcttcag ggcgttcgcc
     6421 atcacaatta ggttttaggt attatgtcaa tcgtttactt gaacaaacat ctcatcaaaa
     6481 aacaaataaa ttaagacgat taaatcaatt gttagttgag aatcaatatg atgtatcatc
     6541 agcattgaca tattttgcag atgaattatc aaatatatct caatatacaa ctttagttgt
     6601 tcatcctaat cataaacaag atattatcaa taatgtacac ttgattcgtg ctaatcctaa
     6661 tttagttata atggttatcg tattttcatc aggtcatgtt gagcatgtac atcttgcttc
     6721 agacataccg tttaataatg acaaactaaa tacaatatca aattttgtta caaacaaact
     6781 aactgagttt aatcagaatt tacaagatga tattgtctct tttgttcaat cagagcagga
     6841 agaaattttt atcaataaat tgttaaatac gatgaataat catatttcta atcaaagtaa
     6901 tagcatttat atgggtggaa aggttaaact tattgatgca ttgaatgaaa gtaatgtatc
     6961 ttcaattcag cccattctac aatatataga atcaaataga attgcagaat tattacaaga
     7021 tatatcttca ccaaatatta atgtaaaaat tggtaatgaa attgatgaca gcttaagtga
     7081 tatttcaatt gtaacaagtc aatatcattt tgatgaaacg ttaaaaggtc aaatcgctgt
     7141 gattggacct acagctatgc attatcaaaa tgtcattcaa ttattaaata gaatttggta
     7201 atgatggatt agaaagtatt ggaggacaga aaatgacaaa taaagacgaa tcagttgaaa
     7261 aaaacactga atcaacagtt gaagaaacaa acgtcaaaca aaatattgat gattcagttg
     7321 aacaagctga agaaagtaaa ggtcatttac aagatgaagc aatagaagaa acgtctgacg
     7381 agaatgttat tgaagaaata gatccaaaag atcaaaaaaa taatgaactt caacaattag
     7441 cagatgaaaa cgaagagaaa tatttaaggc tctacgctga gtttgaaaat tataagcgta
     7501 gaattcaaaa agaaaatgaa ataaacaaaa catatcaagc acaacgtgtg ttaacagata
     7561 ttttaccagc aatagacaat atagaacgtg cacttcaaat tgaaggtgat gatgagactt
     7621 ttaaatctct tcaaaaaggt gtacaaatgg tgcatgaaag tttgattaac gcactaaaag
     7681 ataatggtct tgaagttatt aaaactgaag gtgaagcatt tgatccaaat attcaccaag
     7741 ctgtagttca agatgataac cctgattttg aatctggcga aatcactcaa gaactacaaa
     7801 aaggatacaa gcttaaagat agagtattaa gaccatcaat ggtcaaagta aaccaataac
     7861 ttaaatttgg cgaaaagaca ttgtttaaaa ttaaattaat ttaatgatta aatggaggaa
     7921 ttttattatg agtaaaatta ttggtataga cttaggtaca acaaattcat gtgtaacagt
     7981 attagaaggc gatgagccaa aagtaattca aaaccctgaa ggttcacgta caacaccatc
     8041 tgttgtagct ttcaaaaatg gagaaactca agttggtgaa gtagcaaaac gtcaagctat
     8101 tacaaaccca aacactgttc aatctattaa acgtcatatg ggtactgatt ataaagtaga
     8161 tattgaaggt aaatcataca caccacaaga aatctcagct atgattttac aaaacttaaa
     8221 aaatacagct gaaagctatt taggtgagaa agttgacaaa gctgtaatta cagtacctgc
     8281 atactttaac gatgctgaac gtcaagcaac taaagatgct ggtaaaattg ctggtttaga
     8341 agttgagcgt atcattaatg aaccaacagc tgcagcatta gcatatggtt tagacaaaac
     8401 tgataaagat gaaaaagttc ttgtttttga cttaggtggc ggtacatttg acgtatctat
     8461 cctagaatta ggtgacggtg tattcgaagt actatcaaca gccggtgaca acaaacttgg
     8521 cggtgatgat tttgaccaag taattattga ctacctagtt gcagaattca aaaaagaaaa
     8581 tggcgtagac ttatctcaag ataaaatggc attacaacgt ttgaaagatg ctgctgaaaa
     8641 agctaaaaaa gacttatcag gtgtatcaca aactcaaatc tcattaccat ttatctcagc
     8701 tggtgaaaac ggtccattac acttagaagt aaacttaact cgttctaaat ttgaagaatt
     8761 atcagattca ttaattagaa gaacaatgga acctacacgc caagcaatga aagacgctgg
     8821 cttaacaaac tcagatatcg atgaagttat cttagttggt ggatcaactc gtattccagc
     8881 agtacaagaa gctgtcaaaa aagaaatcgg taaagagcct aacaaaggag taaacccgga
     8941 cgaagtagtg gcaatgggag ctgcaatcca aggtggcgtt atcacaggtg acgttaaaga
     9001 cgtagtatta ttagacgtaa caccactatc tttaggtatt gaaattttag gtggacgtat
     9061 gaatacgtta attgaacgta acactacgat tcctacatct aaatctcaaa tctattcaac
     9121 agcagtagat aatcaaccat cagtagatgt acacgtatta caaggtgaac gtccaatggc
     9181 tgcggataat aaaacacttg gtagattcca attgactgat attccaccag ctgaacgtgg
     9241 taaacctcaa attgaagtaa cgtttgatat cgataaaaac ggtattgtaa atgtaactgc
     9301 aaaagactta ggtacaaata aagaacaaag aattacaatt caatcaagtt cttcattatc
     9361 agacgaagaa atcgaccgta tggtaaaaga tgctgaagtt aacgctgaag cagataaaaa
     9421 acgtcgtgaa gaagtagact taagaaacga agctgacagt ctagtattcc aagttgaaaa
     9481 aactttaact gatttaggcg aaaatatcgg tgaagaagat aaaaaatctg ctgaagagaa
     9541 aaaagacgct cttaaaactg ctttagaagg tcaagatata gaagatatta aatctaaaaa
     9601 agaagaactt gaaaaagtga ttcaagaatt atcagcaaaa gtatatgagc aagcggctca
     9661 acagcaacaa caagcacaag gtgctaatgc tggtcaaaac aatgatagta ctgtagaaga
     9721 tgctgaattt aaagaagtaa aagacgacga caaaaaataa ttaaacaata caattagaat
     9781 gttgtcatga tctgaaaagt caaagtcaat agaacattgg ctttggcttt ttatttggat
     9841 agtatgacat attacgttaa actatatgag ttaattaaga aaaggagaga tagctgtggc
     9901 caaaagagat tattatgaag ttttaggcat tagtaaggat gcttcaaaag atgaaatcaa
     9961 aaaagcgtat cgaaagcttt cgaaaaaata tcatccagat attaacaaag aagaaggtgc
    10021 agatgaaaag tttaaagaaa tttctgaagc ctatgaagtt ttaagtgatg ataataaacg
    10081 cgcaagctat gatcaatttg gacatgatgg tccacaaggt tttggtggac aaggattcaa
    10141 tggctctgac tttggcggtt ttagtggctt cggtggcggc ggttttgaag atatctttag
    10201 ctctttcttc ggtggcggca gacaaagaga tccaaatgca ccgcaaaaag gtgatgatct
    10261 tcagtataca atgacattaa catttgaaga agcggtattt ggtacaacaa aagaaatttc
    10321 aatccgtaaa gatgtaacat gcgaaacatg tcatggtgat ggtgcaaagc ctggaacaag
    10381 taaaaagact tgtagttact gtaatggcgc tggtcatgta gctgtagaac aaaacacaat
    10441 tttaggtaga gttcgtactg aacaagtttg tcctaaatgt aatggaagtg gtcaagaatt
    10501 tgaagaagct tgtccaacat gtcacggaaa aggtactgaa aataaaacag ttaaattaga
    10561 agtgaaagta cctgaaggtg tcgacaatga acaacaaatt agattagctg gtgaaggctc
    10621 tccaggcgtg aatggaggac ctgctggtga tttatacgta gtatttagag ttaaaccatc
    10681 tgaaacattt aaacgtgatg gtgatgatat ttattacaaa ttaaatgtta gcttcccaca
    10741 agcagcttta ggcgatgaaa taaaaatacc aacattaaat aatgaagtta tgttaacaat
    10801 ccctgctggt acacaaacag gtaagcaatt ccgcttaaaa gaaaagggta taaaaaatgt
    10861 tcatggctat ggatacggag atttatatgt tgatattaaa gtagtaacgc ctactaaatt
    10921 aacagataga caaaaagaac taatgaaaga atttgcccaa ttaaatggtg aagaaattaa
    10981 tgaacaacct tcgaatttta aagatagagc aaaaagattc tttaagggag aataaagtat
    11041 gaactggaca gagctttcaa ttattattaa tcatgaagca gtagaattgg ctaccaatat
    11101 acttgaaaat catggatcaa atggtgtcgt gatagaagat tcagatgatt taattaacca
    11161 accagaagat aaatacggtg aaatttacgc tttgaaaaaa gaggattatc cagataaggg
    11221 agtaagattg aaagcctatt ttaatgaaat gacttatgat gataagttgc gacagcaaat
    11281 taaagatgag ttattaaatt tagatgaact tgatcaacat aacgttcaat tcagtgagca
    11341 aattattgca gagacggatt gggaaaatga atggaaaaac tatttccatc cattccgagc
    11401 gtcgaagaag ttcacaatag ttcctagttg ggaaacatat gctaaagaag cggatgaaga
    11461 gctttgcatt gagctcgacc caggtatggc ttttggaaca ggtgatcatc cgactacaag
    11521 tatgtgtttg aaggcaatag aaacatatgt attgccacag cattcagtaa ttgatgttgg
    11581 tactggctca ggtatattaa gtattgcaag tcatctaatc ggtgtaaaac gtattaaagc
    11641 gttggatatt gatgaaatgg cagtgagtgt agctaaagaa aacttcagaa gaaatcattg
    11701 tgaaacgtta attgaagctg ttccaggtaa cttattgaaa gacgaaacag aaaaatttga
    11761 tattgtaata gcaaatattt tagcgcatat tattgatgaa atgattgaag atgcttataa
    11821 tactctaaat gaaggcggtt attttattac ttctggtatt ataaaagaga agtatgaagg
    11881 tatacagtca catatggagc gtgtaggttt taaaattatt tcagaacaac atgacaacgg
    11941 ttgggtttgt cttgttggcc agaaagtgag tgaataatgt gcaacgttat ttcatagacc
    12001 aaaacgctga tgtaagtcag cgttttttta ttacaaaaaa agaagatatt catcatataa
    12061 caaatgtaat gagatatgat gttggtagta aaattatttt aacttttaat gatcaaacag
    12121 tttatttatg tgaaattgct gacataacaa gtgataaaat tgaagttgat ttaaaagaaa
    12181 aacaaaatat taatacagaa ttgccagttg atgttacgat ttgcagtgga ctaatcaaag
    12241 ctgacaaata tgagtggttg ctacaaaaag ctactgaatt gggtgcttca tcatttatag
    12301 ctgtgagcat ggaacgttca attgttaaat taaacgaggc aaaagttgcc aaaaagatag
    12361 agcgttggca aaaaataatt aaagaagctg ctgaacaaag ctatcgttta gtgattccat
    12421 ctattcaatt cgagtcgaat ttaaaattaa tttgtgatac tattgataat tatgactata
    12481 ttcttattgc atatgaagag gaagcaaaag atggtgagtt aagcaatttc aagcaaactt
    12541 tacaacaatt caatgctcag gataaagtgt tgatgatttt tggtcctgag ggtggtttgt
    12601 cagaaaatga aatttcatta tttagtaata ctagtacagt tgttggtctt gggccgagaa
    12661 ttttacgggc agaaactgcg ccattgtatg cgttaagtgc aataagttat gaaaaagaat
    12721 taatggggtg aatatttatg tcaacagttg cgtttcacac attaggttgt aaagtaaacc
    12781 attatgaaac tgaagcaatc tggcaattat ttaaagaagc aaactatgag cgcgttgact
    12841 ttgaagcgaa tgctgatgta tttgttatta atacttgtac agtaacgaat acgggtgata
    12901 aaaaaagtcg tcaaataatt agacgtgcaa taagacaaaa tcctgatgct gtaatctgtg
    12961 taacaggttg ttatgcgcaa acttcatcag ctgaaattat ggaaattcct ggtgtcgatg
    13021 tagtagttgg tacacaagat agacataaac tattaggtta cattgacgaa ttccgtaaag
    13081 aacgccaacc aattaatggt gttggaaata tcatgaaaaa tcgtaaatat gaagaattag
    13141 atgtccctta ttttacagat agaacacgtg cgtcattaaa aattcaagaa ggttgtaaca
    13201 acttctgcac attctgtatt attccatggg ctcgtggctt aatgcgttca agagatccgg
    13261 aaaaagtagt tgaacaagcg acgcaactag tgaattcagg atataaggaa attgtattga
    13321 cgggaattca tacaggtgga tatggtcaag atttaaaaga ttataacttg gcccaattat
    13381 tacgtgatct tgaaacgatt aatggattag aacgaattcg aatttcttca attgaagcaa
    13441 gtcaacttac agatgaagta attgacgttt tagaacgttc aacaaaagtt gtgcgtcatt
    13501 tgcatattcc attacaatct ggttcagata cagtattaaa acgtatgaga cgtaagtata
    13561 caatggatag attttcagaa cgattaacaa aattgcataa agctttacca gacttggcag
    13621 ttacgagtga tgtaattgtt ggtttcccag gtgaaactga agctgagttc caagaaacat
    13681 atgattttat cgtaaagcat aagttctctg aactgcatgt tttcccttat tctcctagaa
    13741 ttggcacgcc agctgcaaga atggatgacc aaattgatga agaaattaaa aatgaacggg
    13801 tgcataagtt aattacgcta agcaatcaac tcggaaagtt atatgcgtct aaatttgatc
    13861 aagatgtgct tgaagtaatt cctgaggaac agggtgacac agaaggtaca ttagttggat
    13921 atgcagataa ttatatgaaa gtacaatttg aaggtgacga atcactcata ggtcaaattg
    13981 taaaagttaa aattacgcaa gcaaattatc cattaaatga agggcaagca attaaagttg
    14041 ttgatttcgc aacaaataaa tctgatagag aagttttagt ttaagtattc cttgattcat
    14101 accatatggt tccgtattaa agatataggt tttgtaacgt tgttttaacg gcttcacaat
    14161 ttactatata agcaactctt ctcattaaat aaattattaa gagattttta gaacaaaatc
    14221 taaaagtctt tgtgattttg atgtgaaata tgtttcaaat aaaaaacaat ttactaattg
    14281 accataaatt acagatatat tatacttata aatgcatagt tttactgtgc aattgactat
    14341 aaagttccgt tgatatttgg agggagggaa atacagatgt ctaaaacagt agtacgtaaa
    14401 aatgaatcac ttgaagatgc gttacgtaga tttaaacgtt cagtttctaa aagtggaaca
    14461 atccaagaag tacgtaaacg tgaattttac gaaaaaccaa gcgtaaaacg taaaaagaaa
    14521 tcagaagctg cacgtaaacg taaattcaaa taattaatac ctctgttgac tccctcaaca
    14581 cgaatattaa ttatataaaa caaacatcac aagttagtgt ctgacacta
//
LOCUS       DAXWFK010000054        14402 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.054, whole genome shotgun sequence.
ACCESSION   DAXWFK010000054 DAXWFK010000000
VERSION     DAXWFK010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14402
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.054"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(49..237)
                     /locus_tag="ACGEXS_001274"
     CDS             complement(49..237)
                     /locus_tag="ACGEXS_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HFF8012292.1"
                     /translation="MNNNEENSVFFGKKKKVSLHLLVDPDMKDEIIKYAQEKDFDNVS
                     QAGREILKKGLEQIKSNK"
     gene            409..1383
                     /gene="trsA"
                     /locus_tag="ACGEXS_001275"
     CDS             409..1383
                     /gene="trsA"
                     /locus_tag="ACGEXS_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer protein TrsA"
                     /protein_id="HFF8012293.1"
                     /translation="MDNITFRKRIEEYRKRSIEQKKYESKLNYQSRFGVKDDEVSTFN
                     IKGIATDKKENKLVMAEDREDKQKVLLYANKDNDMYYLNSGSTYKEAVQNTFGNETNY
                     EMVDDELFIYNSKNQALTNYDQLDEYHNRQKQLQYNTKLEQERKQRLTDTNKVSENAK
                     EDLALSLGLTATALKGNINISNVEKVTDNESMVTIENEINGKSEHLISDFKDSFIEKA
                     KEKLTVSPSMRLGFEEFKNASGQIAMGFTEFFGARYDESTLENDPKVVATEKGYSVSV
                     FDKDGNKKDLTSADTFEEAVKLSKEMKHDNSLSRSEIELNQELEKKKI"
     gene            1400..1717
                     /locus_tag="ACGEXS_001276"
     CDS             1400..1717
                     /locus_tag="ACGEXS_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017466649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CagC family type IV secretion system protein"
                     /protein_id="HFF8012294.1"
                     /translation="MIKKFSLTTVYVAFLSIVLSNITLGAENPGPKIEQGLQQVQTFL
                     TGLIVAVGICAGVWIVLKKLPGIDDPMVKNEMFRGVGMVLAGVAVGAALVWLVPWVYN
                     LFQ"
     gene            1777..2184
                     /locus_tag="ACGEXS_001277"
     CDS             1777..2184
                     /locus_tag="ACGEXS_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012295.1"
                     /translation="MSVEQEVKDIMLDMLGEEKKVKENIIPRNVDASYKIFLNLNGKE
                     LAIVFVPFLLFLTIAFGVTFLMGLLNLITGFMVFIVGLIFGLTIYGLLTIRPISTKEN
                     IRMIDTIKQSQRFSRRQKVYFYKSKEGLGDDDV"
     gene            2171..2854
                     /gene="trsD"
                     /locus_tag="ACGEXS_001278"
     CDS             2171..2854
                     /gene="trsD"
                     /locus_tag="ACGEXS_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrsD/TraD family conjugative transfer protein"
                     /protein_id="HFF8012296.1"
                     /translation="MMMFKKKNKQNKNNEQQFEIINRYIKGDGRNQSIQEDSLIAGFY
                     KDYVVTKDGYISGGLNITGVNTELLTKFEQNDLLEEYSSFISNILYDEPMIKTITEPV
                     NIQNYIKHLYRQVLLDMKNNNNKPTRRGQLIAGRILFLQEKLAKGEMSTKKHYIFFSE
                     KITENTVAQLDHAVNRLHEKINLNKEALQQAFSHYGLETRPIYQTEYLNQFYALYDYK
                     GYNDYHLSN"
     gene            2875..4887
                     /locus_tag="ACGEXS_001279"
     CDS             2875..4887
                     /locus_tag="ACGEXS_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HFF8012297.1"
                     /translation="MAFLKKKKQEQVTNKIHNESFQKLTEVLDTDSVDSIYPFSWIEK
                     KSHIETGENYIKNLLVVDYPQSVKGAYLSNLLKKNGNIQITKFIRPANIERMIDHLNN
                     SIKNKTAEQMRTTDPKRNATIKREIESSKKQLDKFLDEKTGFMYMYMYITLNGDSYEK
                     IQALEKDVKRTLTRLRLKTHTPTNAMRESFHTVLPLNRNFLSAFTQQNMDTATAGHFF
                     MFDDSEIIDLTPNTSVFGINKNTDSLVAVDFNNKEKTLNKNMTIIGTSGVGKSTLNMR
                     MILDNIKKSIRQFIIDPEDEFSYITKYYGGTVVNISTSSNIKINPFEIFSEEVFEKED
                     ETDNETTSDVLTENNEHESQIDTLVRSKIGKLKTFFRVLKDEISQTEISVLSSTLRQL
                     YQDKGFKGNAKLSDFKSEDYPTLTELYNKLKDLDPEKYEVLKDLTLIIEDYTMEHGTT
                     TIFDGYTNIKLDNEIVTFNLKPLQTEKDVQSAAYLNIFSFLWDEITKDRTTETVLMTD
                     ELHFLATNEYSLDFYYQAYKRIRKYGGGAIASTQQIKDILRTSQEIGSAIIENSHTKF
                     FFGMDNVGVDDVVDKLGLKFSDQEISHLTKKKKGEALLLYGTQRAFIRIDLDREETRL
                     WNKELYETIYEEPADVEPNYVEQLGLTDIDLAELEEELRQAEMIYE"
     gene            4899..6179
                     /locus_tag="ACGEXS_001280"
     CDS             4899..6179
                     /locus_tag="ACGEXS_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012298.1"
                     /translation="MKIRNFNEGTLVGKHNHLEYIINVDLCHISDIDEIRYDLTEPNE
                     LFFEVKDLQKEGDYFHIIYNTDNEYNSFLSAKKESKALKLSLMDYVLKVDPLKDNITV
                     MHPANLFFKNIEDIKIGFKGHEYLPKPKINNLEQYKLLIMSTLSQYTFDKYYRNKFEV
                     LSKEKDDFFHKVNNAETFEKLKEIVRHELNQVQTDYLLNEEKRKRETRKKYIRNLILG
                     FVGIAIVISLISFMIINGKQQELDSKIAQADKQEQRSKVYENLYNGNVDQAVKGMKRD
                     DSFNKKDIEKTLKKEKKYEELIELSSKNTPYVIEQLYKEGKQNKIRELAFNFENNDTL
                     SLEKKILDKDGTAFSVGSTGKYEEQSKRLALASAKEGYVESAKDINKKLKDNEVEEEI
                     NKAEIKQLKEEKDSTKDKDKKKEIQKDIEELEKK"
     gene            6197..7273
                     /locus_tag="ACGEXS_001281"
     CDS             6197..7273
                     /locus_tag="ACGEXS_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000269385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HFF8012299.1"
                     /translation="MWSAIAKGITMLMKKKALSKILKPVIAIVIGFFLLIFIMIAYLV
                     GSFTKEINESGEQKMKEAESTATGACTGGSVDGSGISAFEKNAKGGALEGKGKDMVKI
                     AKKNKIPPKLFIAIVASESEWGRGANATKQKNPLSIMGAGPLQVYPSIEEGLDKGAKN
                     LYDLYISEGLTTPEKIGPKYAPVGASNDPDDLNSNWVPTVKKIMKSFGGKEAKCSTES
                     SGGGSDGKGFDFKGEFPKPDKSKYNGQSYPWGQCTWYVHQRRKEIGKPVPLTWGNGGD
                     WGDNAKAQGWEVGSKPKAGAGASVKPGNFGAPPPYGHIMFVEKVKKDGGIVVSEANVK
                     GLGVISSREFSKAETQRMQFIYDK"
     gene            7283..7768
                     /gene="trsH"
                     /locus_tag="ACGEXS_001282"
     CDS             7283..7768
                     /gene="trsH"
                     /locus_tag="ACGEXS_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrsH/TraH family protein"
                     /protein_id="HFF8012300.1"
                     /translation="MKKLLTIVILFTLVLAGCGQNQSEKNSEKHNEEKTVDTTEERND
                     MIKGLIEYGYENDDYKGYKHMKDILSEKLQKQAELQNQSAQTKGVKKETKDIKLYQDV
                     DNDDELIYSIKLSITNKKEKNSDYSMRYGKIELTEEEGKQKIDNVEELTQRNLVSMNQ
                     E"
     gene            7784..9886
                     /gene="topB"
                     /locus_tag="ACGEXS_001283"
     CDS             7784..9886
                     /gene="topB"
                     /locus_tag="ACGEXS_001283"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001094110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFF8012301.1"
                     /translation="MNTLILCEKPSQAMDLSTVFAKKKKQNGYMEISDEQLNVSGFLT
                     WAVGHLVELKEPQEYDEKYKNFSTYPILLEKDDFQFKVSDKTKDQFNNIKKIFKENKI
                     DEVIIATDPAREGENIAYKILNQLKVTDKVTIKRLWLTSKVESSIRKAFKNILPKEKT
                     YGFYKEGRARELSDWLVGINLSRHFTKISRELGNDGVIHIGRVSSPTLNMVYNRENNI
                     KGFKGKKFYKVSATINKDEQEVKTELKNKFDSEDELHEFLFENDITDLTQKGLVTDIE
                     KEIGYTMPPKFYDLSALQEDMNDKYKISAKRTLEIAQTLYEKKLITYPRTDSRYITED
                     EKEMLLENIDYLKEITKINLNNELTNNSLINPSKIEDHYAILITGNDFNKVDLKEEEI
                     NVYKSILQNVAMNFMDKEQYETTTIEIAVKKLMFEVKGKIIQDNGFKALLNKQKTSEE
                     TIPNFEKNEEVDIELDLLEKETTPPKRYTEKTLLKAMANPIETLEDEGLKSTLKEVKG
                     LGTPATRADIIENLKKNKYIQVQKNKIYITKNGILACLLLEGHLLSKPDLTGQWEKYL
                     NGISKGEKDDDSFINTINEMIKKTINEEVKNKESIQKVAKEKVSTNNIAKCPACDNGY
                     LIDRKGFYGCTEYNNGCEFTIPKKLLEKSIPPTVVKALCESKTTKKLKGFKSKKSGKS
                     FDCKLILTKENKLQFSFD"
     gene            9902..10366
                     /locus_tag="ACGEXS_001284"
     CDS             9902..10366
                     /locus_tag="ACGEXS_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000715274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012302.1"
                     /translation="MKKGIFILTGIIWLVLIYLAQILAYALNTLSNTSNKQKIFSDIS
                     QLFIPPAKHPISTIIGFVSDKNTVFFVIGVIATIICIYILIKQLFESKDGNEKNQDYK
                     IAKHGSHGSAKFAEDSELFSEGHYKKMKENDITNYVYNSLDKSLIEERSDKE"
     gene            10363..12003
                     /locus_tag="ACGEXS_001285"
     CDS             10363..12003
                     /locus_tag="ACGEXS_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000209435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFF8012303.1"
                     /translation="MTTLLGELESKATSINKKNLVIQDFDTKCPNRNIFVVGGPGSYK
                     SAGYVIPNVIVKNQQSIVVTDPSGEVYEKTSNIKRMQGFDVRVVNFKNFLASDRQNFF
                     DYIDKDSDCSIVAELIIKSAGDSKINKDVWYKASVGLLNSLLLYAKYEFEPEKRTIGN
                     IIKFIQNNKPNQDEEGSVELDKRFNELSKDHPARESYEFGFAVSEGRTRASIILTFVA
                     DLRNYIDNEIRSYTSDSDFDLRDVGLRKTIIYVMLPVLGNTVQSLSSLFFSQMFQQLY
                     RLGDENGARLPVPVDFLLDEWPNIGAIPDYEETLATCRKYGISITTIVQSISQAVDKY
                     NKDKANAIIGNHAVILCLGNVNNDTAKYIKEELGNATVEFETSSEGSSSGKDSRSKSS
                     NKNVSYTGRALLNEDEISNMQQDKAILITKNRNPKIIKKLAYFKMFPNLTETYIAPQN
                     EYKRKKNQSMIDKHNRLREEWDKKQEKNKQQKLEEYKEEQRQKELEKEQQPQEEQQNE
                     KVKESKDKNDQEDKKDEQQKINDSKKAFYEKLKQKQAK"
     gene            12081..12998
                     /locus_tag="ACGEXS_001286"
     CDS             12081..12998
                     /locus_tag="ACGEXS_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000383805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbL family protein"
                     /protein_id="HFF8012304.1"
                     /translation="MDWIKDAIKELTEGIADGMMNFIFQFLYTLFYEPLKFFIWLLDK
                     ILIDPNSISSDYISNASVLVNSLITSIAICVFAFKMVQVMKQNAEGNAESPGYYVAQL
                     LPSSLLVAVLPWLVDIMTKLSYSATRAFLDVGQKSYLETIKEWSSLKKGQDGFKEMLG
                     SMGTMAGSQIMFILFILLILVFTVVFIFQFVSRIADLVIMKVVAPLVAVSLLADENNY
                     IGVWWRELLAISIQLPLQVFTFFGGINLFFGAKLDVGTLLLGIGFFIITVKSPSFIRS
                     MVYSTGSGRMATGMAGSGAKLLVRKMLSK"
     gene            13015..13407
                     /locus_tag="ACGEXS_001287"
     CDS             13015..13407
                     /locus_tag="ACGEXS_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFF8012305.1"
                     /translation="MINKVIYSGRIATDLKLRKSKNEKSYVFFNIAVPRQFDNDRTDF
                     INCVVWGKQAESLVDYQSKGNLIIIEGSTETYKKDNINMSRCLVEKTIFLESKKENKK
                     HDVSEDDAISPAEDSGNDKSISDDDLPF"
     gene            13464..13631
                     /locus_tag="ACGEXS_001288"
     CDS             13464..13631
                     /locus_tag="ACGEXS_001288"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012306.1"
                     /translation="MKVTNTIRFEEEKKNLIDNVVNTLEEYKDVIDSELRTIRNTNHL
                     VMRNNLVLLQS"
     gene            13669..14343
                     /locus_tag="ACGEXS_001289"
     CDS             13669..14343
                     /locus_tag="ACGEXS_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS257 family transposase"
                     /protein_id="HFF8012307.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRERGVNVHH
                     STVYRWVQEYAPILYQIWKKKHKKAYYKWRIDETYIKIKGKWSYLYRAIDAEGHTLDI
                     WLRKQRDNHSAYAFIKRLIKQFGKPQKVITDQAPSTKVAIAKVIKAFKLKPDCHCTSK
                     YLNNLIEQDHRHIKVRKTRYQSINTAKNTLKGIECIYALYKKNRRSLQIYGFSPCHEI
                     RDMLAS"
BASE COUNT         5959 a         1782 c         2487 g         4174 t
ORIGIN      
        1 ctttactgtt tgtgagttat ttaagtgagg tgttgcctaa tacacaaatt atttattact
       61 tttaatttgt tctaatcctt tttttaagat ttctctacca gcttgtgaaa cgttatcgaa
      121 atctttttct tgtgcgtatt tgataatctc atctttcata tcgggatcaa ctaacaaatg
      181 tagtgatact tttttctttt taccaaaaaa gactgaattt tcttcattat tattcatagt
      241 aatataacct cctttttgtg ctttcgtgta attctattta attacacaat accacattta
      301 cacatgacat gtcatgacaa aaaagttaaa aaagttttat aaattacatg acatgacatg
      361 taatactatg ttataattga tttatcaaat agaaaaggtg ggttgtaatt ggataacata
      421 acatttcgta aacgtattga agaatatcga aagcgatcta ttgagcaaaa gaaatatgag
      481 agcaaactaa attatcaatc aagatttggt gtgaaagatg atgaagttag tacttttaat
      541 atcaaaggca ttgctacaga taaaaaagaa aataagttag tcatggccga agatagagag
      601 gataaacaaa aagttctttt atacgccaat aaagataatg atatgtatta tttgaattct
      661 ggtagcactt ataaagaggc tgttcaaaac acttttggta atgaaaccaa ttacgaaatg
      721 gtagatgatg aattatttat ttataattca aaaaatcaag cacttacaaa ctatgaccaa
      781 cttgatgagt atcacaatcg acaaaagcaa ttacaatata acaccaaatt agaacaggaa
      841 cgaaagcaaa gattgactga tacaaataaa gttagtgaaa atgctaaaga agatttagcg
      901 ttaagtttag gacttacagc aacagcattg aaaggtaaca taaacatttc taatgttgaa
      961 aaagtcactg ataatgaaag tatggttact atagaaaatg aaattaatgg taagtcagaa
     1021 catttaattt cagactttaa agatagtttc attgaaaagg ctaaagaaaa gctaactgtt
     1081 tccccttcaa tgaggttagg atttgaagaa tttaaaaacg cctcgggaca aatagcaatg
     1141 ggatttacag agttttttgg cgctaggtat gacgaaagca ctttagaaaa cgaccctaaa
     1201 gttgtggcaa cagaaaaagg gtactctgtt tctgtttttg ataaagacgg aaacaaaaaa
     1261 gatttaacat cagctgacac ttttgaggag gcagtaaaat tatccaaaga aatgaaacat
     1321 gataactcgc tttctcgtag tgaaatagaa ttaaatcaag aattagaaaa gaaaaaaata
     1381 taaggaaggt tgattaatta tgattaaaaa attcagttta acaactgttt atgtagcatt
     1441 tttaagcatt gttttatcaa atataacact tggcgcagaa aatccagggc cgaaaattga
     1501 acaaggttta caacaagtac aaacattctt aacaggccta attgttgctg ttggtatctg
     1561 tgctggtgtt tggatagttc ttaaaaaatt acctggaatt gatgatccaa tggtaaaaaa
     1621 tgaaatgttt agaggcgttg gaatggtatt agctggtgtg gctgttgggg cagcacttgt
     1681 ttggttggtt ccatgggtat acaacctttt ccaataaaca caacaaaagc tacattctag
     1741 taatgtagct ttttgtttta aaaaggagtg aattatatgt ctgttgagca agaagttaaa
     1801 gatataatgc tcgatatgtt gggcgaagaa aagaaagtta aagagaatat aatacctcgt
     1861 aatgtagacg ctagttataa aatattctta aatttaaacg gtaaagaatt agcaattgta
     1921 tttgttcctt ttttactatt tctaacaata gcatttggtg taacgttttt aatgggctta
     1981 cttaatttaa taactggctt tatggttttt atagtaggtt tgatttttgg tttaacaata
     2041 tatggcttac taacaattag accaattagt acaaaagaaa atataagaat gattgatacc
     2101 ataaaacaat cacaacgatt tagcagaagg cagaaagttt acttctataa aagtaaagaa
     2161 ggattgggtg atgatgatgt ttaaaaaaaa gaataagcaa aataaaaata acgaacaaca
     2221 atttgaaatt ataaatagat atataaaagg ggacggacgt aatcaaagta ttcaagaaga
     2281 ttcactaata gctggttttt ataaagatta cgttgttact aaagacggtt atatcagtgg
     2341 aggtttaaat attactggtg taaatacaga gttattaacg aagtttgagc aaaatgattt
     2401 attagaagaa tattcatctt ttatttcaaa cattctttac gacgaaccaa tgataaaaac
     2461 aattacagaa ccagtaaata tacaaaatta tataaaacac ctttacagac aagtattact
     2521 tgatatgaag aataataata ataaaccaac aagacgtggc caattaatag ctggtcgtat
     2581 attgtttctt caagaaaagc tagcaaaagg cgaaatgagt acaaagaaac attatatttt
     2641 cttttcggaa aagataacag aaaatactgt agcacaatta gatcatgcag tgaatagact
     2701 acatgaaaag ataaacctta ataaagaggc attacaacaa gcattttcac attatggttt
     2761 agaaactaga cctatatatc aaacagaata cttaaaccag ttttatgctt tatatgatta
     2821 taaaggttat aacgactatc atcttagtaa ttagaacgaa gggagtttat gattatggca
     2881 tttttaaaaa agaaaaagca agagcaagtt acaaataaaa ttcacaatga atcttttcaa
     2941 aaattaacag aagtattaga tacagattct gttgatagta tttatccttt tagttggata
     3001 gagaaaaaat cgcatataga aacaggcgaa aattatatta aaaatttatt agtagttgac
     3061 taccctcaat cagtaaaagg ggcgtactta tcaaatttat taaagaaaaa cggtaatatt
     3121 caaataacta aatttattag accagcaaat atagaaagaa tgattgacca tttaaacaac
     3181 tcaatcaaaa acaaaacagc agaacaaatg cgtactacag accctaaacg aaatgcaaca
     3241 attaaaagag aaattgaaag tagtaaaaaa caacttgata aatttttaga tgaaaaaaca
     3301 ggatttatgt acatgtatat gtatatcacg ttaaatggcg attcatacga aaagatacaa
     3361 gcgctagaaa aagatgttaa gcgtacttta acgagattaa gacttaaaac acatacacct
     3421 acaaacgcaa tgcgagaatc atttcataca gttcttcctt taaatagaaa cttcttatca
     3481 gcatttacac aacaaaatat ggatacagca acagcagggc atttctttat gtttgatgat
     3541 tcagaaatta ttgatttaac acctaataca tcagtttttg gaattaacaa aaatacagat
     3601 tcattagtag cagttgattt taataacaaa gaaaaaactt taaataaaaa tatgactatt
     3661 atcggtacaa gtggtgtagg taaatctacc ctcaatatga gaatgatttt agacaatatc
     3721 aaaaaatcta taagacaatt tatcattgat cccgaagatg aattttcata tataactaaa
     3781 tattacggtg gaacagttgt aaatatttca acaagttcaa atatcaaaat taatccattt
     3841 gaaatttttt cagaagaagt atttgaaaaa gaagatgaaa cagacaatga aacaacatca
     3901 gatgttttaa cggaaaacaa cgaacacgaa agccaaattg acacattagt tcgttctaaa
     3961 atagggaagt taaaaacctt ctttagagtt cttaaagatg aaatatctca aacagaaata
     4021 tctgtattat cttcaacttt aagacagtta tatcaagata aaggatttaa aggtaatgcg
     4081 aaattatcag atttcaaaag cgaagattat ccaactttaa cagaattata taacaaacta
     4141 aaagacttag acccagaaaa atatgaggta ttaaaggact taacacttat catagaagat
     4201 tacacaatgg aacatggtac tactacaatc tttgacggtt atacaaacat caaattagat
     4261 aatgaaattg ttacttttaa ccttaaacca ttacaaacag aaaaagatgt tcaaagtgca
     4321 gcgtatttga atatattcag tttcttatgg gacgaaataa caaaagatag aacaacagaa
     4381 acagtattaa tgactgacga attacatttc ttagcaacaa atgaatatag cttagatttt
     4441 tattatcaag catacaagcg tattagaaaa tatggcggtg gcgctattgc ctctactcaa
     4501 caaattaaag atattcttag aacatcacaa gaaataggtt cagctattat tgaaaatagt
     4561 catactaaat tcttctttgg aatggataac gtaggcgttg atgatgttgt tgataaatta
     4621 ggattgaaat ttagcgatca agaaatatct catttaacta aaaagaaaaa aggcgaggca
     4681 ttattactat acggaacaca aagagcattt ataagaattg atttagatag agaagaaaca
     4741 agactttgga ataaagaatt atatgaaaca atttatgaag aaccagcaga cgtagagccg
     4801 aattatgttg aacaactagg attaacagat attgatttag cagagttgga agaagaatta
     4861 agacaagcag aaatgattta tgaataggag gtaatctcat gaaaattaga aattttaatg
     4921 aaggaacatt ggtaggaaaa cataaccact tagaatatat catcaatgtt gatttgtgcc
     4981 atatatcaga tatagacgaa attagatatg acttaacaga acctaatgaa ttgttttttg
     5041 aagttaaaga tttacaaaag gaaggcgatt attttcatat catttacaac actgataatg
     5101 aatataattc cttcctatca gcaaaaaaag aaagcaaagc actcaaatta tctttaatgg
     5161 attatgtgtt aaaagttgat ccattaaagg acaatataac tgttatgcac ccagcgaacc
     5221 tattttttaa aaatattgag gatataaaaa taggattcaa aggccatgaa tatttaccta
     5281 aacctaaaat aaataattta gaacagtata aattgttaat aatgagtaca ttatcacaat
     5341 acacgtttga taagtattat cgcaataaat ttgaagtttt atcaaaagaa aaagatgatt
     5401 ttttccataa agtaaataat gcagaaactt ttgaaaaatt gaaagagatt gtaagacatg
     5461 aattgaatca agtacagaca gattatttac tgaatgaaga aaaacgtaaa agagaaacac
     5521 gaaaaaagta cattagaaat ttaatacttg gttttgttgg tattgcaata gttataagtt
     5581 taatttcatt tatgattata aacggaaagc aacaagaatt agatagcaaa atagcacaag
     5641 cagacaaaca agaacaaaga agtaaagtat atgaaaatct ttacaatggc aatgttgatc
     5701 aagcagttaa aggcatgaaa cgtgacgata gctttaacaa aaaagatatt gaaaaaacat
     5761 taaaaaaaga aaagaaatat gaagaactaa ttgaattgag tagtaaaaac acaccttatg
     5821 taattgaaca attatataaa gaaggtaagc agaataaaat aagagagtta gctttcaatt
     5881 ttgaaaataa cgatacattg agtttagaaa agaaaatatt agacaaagac ggaacagcat
     5941 ttagtgtagg ctcaacaggt aaatacgaag aacaaagtaa acgtttggct ttagcgtcag
     6001 ctaaagaagg ttatgttgaa tcagctaaag atattaataa aaaacttaaa gataatgaag
     6061 ttgaagaaga aataaataaa gctgaaatta aacaactaaa agaagaaaaa gacagcacga
     6121 aggataaaga caagaaaaaa gaaatccaaa aagatataga ggaattagag aagaaatagg
     6181 agggaggtaa agtatcatgt ggagtgctat tgctaaagga ataacaatgc tgatgaagaa
     6241 aaaagcatta tccaaaatat taaaaccagt aatagctata gtaataggct ttttccttct
     6301 tatttttata atgattgctt atctagtagg atctttcact aaagaaatta atgagagtgg
     6361 cgaacaaaaa atgaaagagg ctgaatcaac agcaacaggt gcgtgtactg gtggaagtgt
     6421 tgacggttcg ggtatctctg cttttgaaaa aaacgccaaa ggtggcgcat tagaaggtaa
     6481 aggtaaggat atggttaaaa ttgctaaaaa aaataaaata ccaccaaaat tatttatagc
     6541 aattgtagct tctgagtcag aatggggaag aggggcaaat gcaacaaaac aaaaaaaccc
     6601 cctatctata atgggggctg gccctctaca agtttaccct tcaattgaag aaggacttga
     6661 taaaggtgca aaaaatttat atgacttata catttctgaa ggtttgacaa ctcccgagaa
     6721 aatcgggcca aaatacgcac cagtaggcgc tagtaatgac ccagatgact taaatagtaa
     6781 ctgggtacct acagttaaga aaatcatgaa gtcattcggt ggcaaagagg ctaaatgttc
     6841 tactgaaagc agtggtggag gttcagacgg aaaaggattt gattttaaag gagaattccc
     6901 taaacctgac aaatcgaaat ataacggtca atcttatcca tggggacagt gtacttggta
     6961 tgtacatcaa cgtagaaaag aaattggcaa accagtacct ttaacttggg gtaatggtgg
     7021 ggactggggc gataatgcaa aagcgcaagg ctgggaagta ggtagtaagc cgaaagctgg
     7081 cgctggcgcc tctgtaaaac ccggtaactt tggcgcacca ccaccgtatg gacacatcat
     7141 gtttgtcgaa aaagttaaaa aagacggtgg aattgtagtt agtgaggcca atgtaaaagg
     7201 cctaggagtt ataagttcaa gagaattttc aaaagcagaa acacaacgta tgcaatttat
     7261 ttatgataag taggtgtaaa aaatgaaaaa attattaact atagttattt tattcacttt
     7321 ggttctggct ggttgtggcc aaaatcaaag tgagaaaaat agtgaaaaac ataatgaaga
     7381 aaaaacagta gatactacag aagaaagaaa cgatatgata aaaggtctaa ttgaatatgg
     7441 ttatgaaaat gatgattata aaggttataa gcatatgaaa gatatattat ctgaaaagtt
     7501 gcaaaagcaa gcagaattgc aaaatcaatc agcacaaact aaaggtgtaa aaaaagaaac
     7561 taaagacatt aaactttatc aagacgtaga taatgatgat gaattgattt attctattaa
     7621 actttcaatt acaaacaaaa aagagaaaaa ctctgactac tcaatgagat atgggaaaat
     7681 tgaattaacg gaagaagaag gtaaacaaaa gatagataat gtcgaagaac taacacaaag
     7741 aaatttagta agtatgaatc aagaatagaa agaggtaata cttatgaaca ctttaattct
     7801 atgtgaaaaa ccttcacaag caatggactt aagcactgtt tttgcaaaaa agaaaaagca
     7861 aaatggatat atggaaattt cagatgaaca attaaatgta agtggctttt taacttgggc
     7921 agttggtcat ttagtcgaat tgaaggaacc acaagaatat gacgaaaaat ataaaaattt
     7981 tagcacatat cctatattgt tagaaaaaga cgattttcaa tttaaagtat ctgacaaaac
     8041 gaaagatcaa tttaacaaca ttaaaaaaat atttaaagaa aacaagattg atgaagttat
     8101 tattgctact gacccagcta gagaaggcga aaatatagca tataaaattt taaatcaatt
     8161 gaaagtaaca gataaagtta ctataaaaag attgtggctt acttctaaag tagaaagcag
     8221 tataagaaag gcttttaaaa atatattacc taaagaaaaa acttatggat tttacaaaga
     8281 gggcagggct agagaattaa gtgattggtt agtaggaatt aacttatcac gtcatttcac
     8341 taaaatttca cgagaattag gaaatgacgg tgttattcat atcggtagag tatctagtcc
     8401 tacattaaac atggtttaca acagagagaa taacattaaa ggttttaaag gtaaaaagtt
     8461 ttataaagtt tcagcgacta ttaacaaaga tgaacaagaa gtgaaaacag agttaaaaaa
     8521 caaatttgac agtgaagatg aattgcacga atttttgttt gaaaatgata ttacagattt
     8581 aacacaaaaa gggttagtta ctgatattga aaaagaaata ggctatacaa tgccacctaa
     8641 attttatgac ttatcagctt tacaagaaga tatgaatgat aaatataaaa taagtgcgaa
     8701 acgaacatta gaaatcgcac agacattata tgagaagaaa ttaatcacat atccaagaac
     8761 tgattcaaga tatatcacag aagatgaaaa agaaatgtta ttagaaaata tagactattt
     8821 aaaagaaata acaaagatta atttgaataa tgaattaact aataacagct tgattaatcc
     8881 ttctaaaata gaagatcatt acgcaatatt aataactgga aatgatttta acaaagtaga
     8941 tttaaaagaa gaagaaatca atgtttataa aagtatatta caaaatgtgg ccatgaattt
     9001 tatggataaa gaacaatatg aaacgactac tatagaaatc gctgttaaaa agcttatgtt
     9061 tgaagtgaaa ggtaaaatta ttcaagataa cggatttaaa gctttgttaa ataaacaaaa
     9121 aacttccgaa gaaacaattc ctaattttga aaaaaatgaa gaagtagaca ttgaattaga
     9181 tttgttagaa aaagaaacga cacctccaaa aagatataca gaaaaaacat tgttaaaagc
     9241 aatggccaat cctatagaaa cactagaaga tgaaggctta aagtctacac ttaaagaagt
     9301 taaaggttta ggcacaccag caactagagc cgatattata gaaaacttga agaaaaataa
     9361 atatattcaa gtacaaaaaa acaaaattta tattactaaa aatggtatat tagcttgttt
     9421 attattggaa ggacatttac tttcaaaacc agacttaaca ggacaatggg aaaaatattt
     9481 gaatggtatt agtaaaggcg aaaaagatga tgattcattt ataaatacca ttaatgaaat
     9541 gattaaaaaa acaattaatg aagaagttaa aaataaagaa agtatacaaa aagtggccaa
     9601 agagaaagta tcgactaaca atatagcaaa atgccctgct tgcgataatg gctatttaat
     9661 agatagaaaa ggtttttatg gttgtacaga gtacaataac ggttgtgaat ttactattcc
     9721 taaaaaattg ttagaaaaaa gtatacctcc aactgtagta aaagcattat gtgaaagtaa
     9781 gacaacaaaa aaattgaaag gctttaaatc aaaaaaaagt ggaaagtcat ttgattgtaa
     9841 gttgatatta acaaaagaaa acaaacttca attttcattc gattaaaagg aggataagaa
     9901 agtgaaaaaa ggaatattca ttttaactgg tattatttgg ctagtattaa tatatttagc
     9961 gcaaatatta gcatatgctt taaatacact aagtaatact agcaataagc aaaagatatt
    10021 tagtgatata tcacagttgt ttatacctcc agctaaacac ccaataagca ctattatcgg
    10081 ttttgtatct gataaaaata cagtgttttt tgtaataggt gtaatagcaa caattatttg
    10141 tatatatatt cttataaaac aattatttga gagcaaagac ggaaacgaaa aaaatcaaga
    10201 ctataaaatt gctaaacatg gttctcatgg atcagctaaa tttgccgaag atagcgaatt
    10261 gttttcagaa ggacattata aaaaaatgaa agaaaacgac attacgaact atgtttataa
    10321 cagtttagat aaatcattaa tagaagaaag aagtgataag gaatgactac acttttaggc
    10381 gaattagaaa gtaaagcaac ttctataaat aaaaagaatc tagtaataca agattttgat
    10441 actaaatgcc ctaatagaaa tatatttgtg gtaggagggc caggttccta taagtcagct
    10501 gggtatgtta ttcctaatgt tattgttaaa aatcaacaat caatagttgt aactgaccct
    10561 agtggcgaag tttatgaaaa aacatctaat attaaacgca tgcaaggttt tgatgttaga
    10621 gtagtaaatt tcaaaaactt tttagcctct gatagacaaa acttttttga ttatattgat
    10681 aaagattctg attgttcaat agttgctgaa ttaattatta aatcagctgg ggattctaaa
    10741 ataaataaag acgtatggta taaagcctct gtaggattat taaattcatt gcttttatac
    10801 gctaaatatg aatttgaacc agaaaaacga actataggaa acattattaa atttattcaa
    10861 aataacaaac ctaatcaaga tgaagaagga tcagttgaat tagataaacg ctttaatgaa
    10921 ttatctaaag accacccagc tagagaaagt tatgaatttg gttttgcagt atcagaagga
    10981 agaacaagag caagtattat actaaccttt gtagccgatt tacgaaatta cattgataac
    11041 gaaattagaa gttatacaag cgattctgat tttgatttac gagatgtagg attaagaaaa
    11101 actattatat atgtaatgtt gccagttttg gggaacactg ttcaatcact ttctagttta
    11161 ttcttctctc aaatgttcca acagctatat aggttagggg acgaaaacgg tgctagactt
    11221 ccagtcccag tagatttttt actagacgaa tggccaaata tcggcgcaat accagactat
    11281 gaagaaactt tagcaacttg tcgtaagtat ggtattagta tcacaacaat tgttcaatct
    11341 atatcacaag cagtagataa atataataaa gataaagcta acgctattat aggtaaccac
    11401 gcagttattt tatgtttagg taatgtgaat aatgatacag caaaatatat aaaagaagaa
    11461 ttaggtaatg ctactgtaga gtttgaaaca agtagtgaag gttcttctag tggtaaagat
    11521 agcagaagta aatcatcaaa taaaaatgta agctacactg gaagggcatt actaaatgaa
    11581 gatgaaataa gtaatatgca acaggataaa gctattctta taactaaaaa tagaaatcct
    11641 aaaattatta aaaagttagc atattttaaa atgttcccta atttaacaga aacgtatata
    11701 gcacctcaaa acgaatacaa acgtaaaaaa aaccagtcta tgattgataa gcataataga
    11761 ttaagagaag aatgggataa aaaacaagag aaaaataaac agcaaaaact agaagaatat
    11821 aaagaagaac aaagacagaa agagttagag aaagaacaac aaccacaaga agaacaacaa
    11881 aatgagaaag taaaagaaag taaagacaaa aacgatcaag aagataagaa agacgagcaa
    11941 caaaaaatta atgattctaa gaaagcattc tacgaaaaat taaaacaaaa acaagctaaa
    12001 taaaagtaag attttgcatt acatgacatg acatgtaatg caaaatctgt tatacttgta
    12061 ttgaaagtag aggtggttga atggattgga ttaaagacgc tattaaagaa ctgaccgaag
    12121 gtattgcaga cggtatgatg aattttatat ttcagtttct ttatacgtta ttttacgaac
    12181 ctttaaaatt ctttatatgg ttgttagata aaatacttat tgatcctaat agtatttcaa
    12241 gtgattacat ttcaaacgcc tctgtattag ttaatagctt aatcacttca atagcaatat
    12301 gtgtgtttgc ttttaaaatg gttcaagtta tgaaacaaaa tgcagaaggt aatgctgaaa
    12361 gtccaggcta ttatgtagca caattattac caagttcttt attggtagca gttttaccat
    12421 ggctagttga tataatgact aaattatctt attcagcgac tagggcattt ttagatgtag
    12481 gtcaaaaaag ttatttagaa actattaaag aatggagttc tttaaaaaaa ggtcaagacg
    12541 gatttaaaga aatgctaggt tctatgggga ctatggcagg tagtcaaatt atgtttattc
    12601 tatttattct attgatttta gtatttactg tagtatttat tttccaattt gttagtagga
    12661 tagctgattt agtaataatg aaagttgttg ccccgttagt agcagtttct ttattagcag
    12721 atgaaaataa ttatataggt gtatggtggc gtgaattatt agcaattagt attcaattac
    12781 cattacaagt gtttactttc tttggaggaa taaatttatt ctttggtgca aagctagacg
    12841 taggcacatt attattaggt ataggattct ttattattac agttaaatcg ccttccttta
    12901 ttagatcaat ggtttactct actggaagtg ggcgcatggc aactggaatg gctggaagtg
    12961 gtgcaaaact tctagttaga aaaatgttat ctaaataaag aaaggaagat atttatgata
    13021 aacaaagtaa tatatagtgg acgtatcgca actgatttaa aattaagaaa aagtaaaaat
    13081 gaaaagtctt atgttttctt taacatagct gtacctaggc agtttgataa tgatagaaca
    13141 gattttatta attgtgttgt atggggaaaa caagcagaaa gtttagttga ttatcaaagt
    13201 aaaggtaatc taattattat tgaaggttca actgaaacat ataaaaaaga caatattaat
    13261 atgtctagat gtttggttga aaagactatc tttttagaaa gcaaaaaaga aaacaaaaaa
    13321 catgacgtgt cagaagatga cgctataagc ccagcagaag atagtggcaa cgataaatct
    13381 atctctgatg atgacttacc attttaaaac tataaaagga cgtgtgaaaa cacgttcttt
    13441 tttaaaaaat aggagtgatt tagatgaaag taacaaatac aattagattt gaagaagaaa
    13501 agaaaaactt aattgataat gtagtaaata cactagaaga atataaagat gttatcgaca
    13561 gtgaattgcg tactattaga aacactaatc atttagttat gagaaataat ttggttctgt
    13621 tgcaaagttg aatttatagt ataattttaa caaaaaggag tcttctgtat gaactatttc
    13681 agatataaac aatttaacaa agatgttatc actgtagccg ttggctacta tctaagatat
    13741 gcattgagtt atcgtgatat atctgaaata ttaagggaac gtggtgtaaa cgttcatcat
    13801 tcaacagtct accgttgggt tcaagaatat gccccaattt tgtatcaaat ttggaagaaa
    13861 aagcataaaa aagcttatta caaatggcgt attgatgaga cgtacatcaa aataaaagga
    13921 aaatggagct atttatatcg tgccattgat gcagagggac atacattaga tatttggttg
    13981 cgtaagcaac gagataatca ttcagcatat gcgtttatca aacgtctcat taaacaattt
    14041 ggtaaacctc aaaaggtaat tacagatcag gcaccttcaa cgaaagtagc aatagctaaa
    14101 gtaattaaag cttttaaact taaacctgac tgtcattgta catcgaaata tctgaataac
    14161 ctcattgagc aagatcaccg tcatattaaa gtaagaaaga caagatatca aagtatcaat
    14221 acggcaaaaa atacactcaa aggcattgaa tgtatttacg ctctatataa aaagaaccgc
    14281 agatctcttc agatctacgg attttcgcca tgccacgaaa ttagggatat gttagccagt
    14341 taagggaaca acgacatgat aaaatggtag ttagctatat ttttctaact ttgcaacaga
    14401 ac
//
LOCUS       DAXWFK010000055        14234 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.055, whole genome shotgun sequence.
ACCESSION   DAXWFK010000055 DAXWFK010000000
VERSION     DAXWFK010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14234
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.055"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..542
                     /locus_tag="ACGEXS_001290"
     CDS             <1..542
                     /locus_tag="ACGEXS_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012308.1"
                     /translation="TGFNLYRHQMQHLSLSAMLLSITINAFINTFIIDKYNFITLCTI
                     TMFIIWTILQFYIDKKLKPVYITDQKFIAIILTIVVSLTQRVTDFSSTQSIYMSIPFL
                     APAIFIIGGIMLFISTFNSLDETAENNNKIKKLMIKGLIIINISFIVMMVLTPYWYLY
                     LIVYLIFLLFLLWQKVYKF"
     gene            822..1262
                     /locus_tag="ACGEXS_001291"
     CDS             822..1262
                     /locus_tag="ACGEXS_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HFF8012309.1"
                     /translation="MNTKYFLAAGAVITTLALGACGNSNSQDQGNKTEQKTKSEDSNV
                     KTDKTKHLTGTFSSKNGETVEGKAEIKNGKLMLTNYKSSKGPDLYVYLTKNGDIKNGK
                     EIAMVDYDKEKQTFDLKNVDLSKYDEVTIYCKKAHVIFGGAKLK"
     gene            1604..2077
                     /locus_tag="ACGEXS_001292"
     CDS             1604..2077
                     /locus_tag="ACGEXS_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HFF8012310.1"
                     /translation="MNKLLLLVTFIIRVGSGIVMLMQGYEKLTGGFTLKGLVPVIANN
                     TDSPEWYKWFFANIVAHTTSLFDIVVPLGEIAIGLGLIFGVFAYAASFFGAFVMINYI
                     LADMIFTYPLQLTFFILLLMSHSLLKQISLKEIINYFRGRKNRGEKIDDPLTDRG"
     gene            2052..2738
                     /gene="saeR"
                     /locus_tag="ACGEXS_001293"
     CDS             2052..2738
                     /gene="saeR"
                     /locus_tag="ACGEXS_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor SaeR"
                     /protein_id="HFF8012311.1"
                     /translation="MTHLLIVDDEQDIVDICQTYFEYEGYKVTTTTSGKEAISLLSND
                     IDIMVLDIMMPEVNGYDIVKEMKRQKLDIPFIYLTAKTQEHDTIYALTLGADDYVKKP
                     FSPRELVLRINNLLTRMKKYHHQPVEQLSFDELTLINLSKVVTVNGHEVPMRIKEFEL
                     LWYLASRENEVISKSELLEKVWGYDYYEDANTVNVHIHRIREKLEKESFTTYTITTVW
                     GLGYKFERSR"
     gene            2738..3793
                     /gene="saeS"
                     /locus_tag="ACGEXS_001294"
     CDS             2738..3793
                     /gene="saeS"
                     /locus_tag="ACGEXS_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SaeS"
                     /protein_id="HFF8012312.1"
                     /translation="MVLSIRSQIIIGVVSSILLTSTILAIAYILMWFNGHMTLTLTLT
                     TIITSCLTLLICSIFINPLIQKIKQFNIKTKQFANGNYASNDKTFNSPKEIYELNQSF
                     NKMASEITQQMNQIKSEQQEKTELIQNLAHDLKTPLASIISYSEGLRDGIITKDHEIK
                     ESYDILIKQANRLSTLFDDMTHIITLNTGKTYPPELIQLDQLLVSILQPYEQRIKHEN
                     RTLEVNFCNEIDAFYQYRTPLERILTNLLDNALKFSNVGSRIDINISENEDQDTIDIA
                     ISDEGIGIIPELQERIFERTFRVENSRNTKTGGSGLGLYIANELAQQNNAKISVSSDI
                     DVGTTMTVTLHKLDITS"
     gene            complement(3865..4848)
                     /gene="csbB"
                     /locus_tag="ACGEXS_001295"
     CDS             complement(3865..4848)
                     /gene="csbB"
                     /locus_tag="ACGEXS_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid-specific glycosylation protein
                     CsbB"
                     /protein_id="HFF8012313.1"
                     /translation="MKIRVVIPCFNEGEVITQTHQQLTEILSQDSSVKGYDYNMLFID
                     DGSTDTTIDEMQHLATIDRHVSFISFSRNFGKEAAMIAGYQHSTEFDAVIMIDCDLQH
                     PPEYIPKMVEGFMEGYDQVIAKRDRSGENFSRKTLSHLYYKLVNCFVEEVQFDDGVGD
                     FRLLSQRAVKSIASLEEYNRFSKGLFEWIGYNTKVFTYQNVERQKGESKWSFKKLFNY
                     GIDGLISFNSKPLRMMIYLGLFIFSISVLYIIYLFINIMISGVNIPGYFSTIAAILLL
                     GGIQLISIGVVGEYIGRIYYEVKARPKYIIQATNLSSLENDEKDTHKVYSK"
     gene            complement(4980..5819)
                     /locus_tag="ACGEXS_001296"
     CDS             complement(4980..5819)
                     /locus_tag="ACGEXS_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFF8012314.1"
                     /translation="MLNEIQILNNGYPMPSVGLGVYKISDEDMTKVVNAAIDAGYRAF
                     DTAYFYDNEASLGRALKDNGVDREDLFITTKLWNDYQGYEKTFEYFNKSIENLQTDYL
                     DLFLIHWPCEADGLFLETYKAMEELYEQGKVKAIGVCNFNVHHLEKLMAQSSIKPMVN
                     QIEVHPYFNQQELQEFCDRHDIKVTAWMPLMRNRGLLDDPVIVKIAEKYHKTPAQVVL
                     RWHLAHNRIIIPKSQTPKRIQENIDILDFNLELTEVAEIDALNRNARQGKNPDDVKIG
                     DLK"
     gene            complement(6041..7390)
                     /locus_tag="ACGEXS_001297"
     CDS             complement(6041..7390)
                     /locus_tag="ACGEXS_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFF8012315.1"
                     /translation="METSTIISLIIFILLIALTTVFVGSEFALVKIRATRIEQLADEG
                     NKPAKIVKKMIANLDYYLSACQLGITVTSLGLGWLGEPTFEKLLHPIFEAINLPTALT
                     TTISFAVSFIIVTYLHVVLGELAPKSIAIQHTEKLALVYARPLFYFGNIMKPLIWLMN
                     GSARVIIRMFGVNPDAQTDAMSEEEIKIIINNSYNGGEINQTELAYMQNIFSFDERHA
                     KDIMVPRTQMITLNEPFNVDELLETIKEHQFTRYPITDDGDKDHIKGFINVKEFLTEY
                     ASGKTIKIANYIHELPMISETTRISDALIRMQREHVHMSLIIDEYGGTAGILTMEDIL
                     EEIVGEIRDEFDDDEVNDIVKIDNKTFQVNGRVLLDDLTEEFGIEFDDSEDIDTIGGW
                     LQSRNTNLQKDDYVDTTYDRWVVSEIDNHQIIWVILNYEFNEARPTIGQSDEDEKSE"
     gene            complement(7611..8792)
                     /gene="nagA"
                     /locus_tag="ACGEXS_001298"
     CDS             complement(7611..8792)
                     /gene="nagA"
                     /locus_tag="ACGEXS_001298"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFF8012316.1"
                     /translation="MSELIIYNGKVYTEDGKIDNGYIHVKDGQIVAIGEVDDKAAIDN
                     DTTNKIQVIDAKGHHVLPGFIDIHIHGGYGQDAMDGSYDGLKYLSENLLSEGTTSYLA
                     TTMTQSTDKIDNALTNIAKYEAEQDVHNAAEIVGIHLEGPFISENKVGAQHPQYVVRP
                     FIDKIKHFQETANGLIKIMTFAPEVEGAKEALETYKDDIIFSIGHTVATYEEAVEAVE
                     RGAKHVTHLYNAATPFQHREPGVFGAAWLNDALHTEMIVDGTHSHPASVAIAYRMKGN
                     ERFYLITDAMRAKGMPEGEYDLGGQKVTVQSQQARLANGALAGSILKMNHGLRNLISF
                     TGDTLDHLWRVTSLNQAIALGIDDRKGSIKVNKDADLVILDDDMNVKSTIKQGKVHTF
                     S"
     gene            complement(9100..11058)
                     /locus_tag="ACGEXS_001299"
     CDS             complement(9100..11058)
                     /locus_tag="ACGEXS_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFF8012317.1"
                     /translation="MRVTELLTKDTIAMDLMANDKNGVIDELVNQLDKAGKLSDVASF
                     KEAIHNRESQSTTGIGEGIAIPHAKVAAVKSPAIAFGKSKAGVDYQSLDMQPAHLFFM
                     IAAPEGGAQTHLDALAKLSGILMDENVREKLLHASSPEEVLAIIDEADDEVTKEEEAE
                     AEAQQVATAEQSSKQSNEPYVLAVTACPTGIAHTYMARDALKKQADKMGIKIKVETNG
                     SSGIKNHLTEQDIENATGIIVAADVHVETDRFDGKNVVEVPVADGIKRPEELINKALD
                     TSRKPFVARDGQRKGNSNDSQEKLSPGKAFYKHLMNGVSNMLPLVISGGILMAIVFLF
                     GANSFNPKSSEYNAFAEQLWNIGSKSAFALIIPILSGFIARSIADKPGFASGLVGGML
                     AISGGSGFIGGIIAGFLAGYLTQGVKAMTRKLPQALEGLKPTLIYPLLTVTATGLLMI
                     YAFNPPASWLNHLLLDGLNNLSGSNIVLLGLVIGAMMAIDMGGPFNKAAYVFATGALI
                     EGNAAPITAAMIGGMIPPLAIATAMLIFRRKFTKEQRGSIIPNYVMGMSFITEGAIPF
                     AAADPLRVIPSMMIGSGIGGAIALGLGSRITAPHGGIIVIVGTDGAHLLQTLIALLVG
                     TLVSALIYGLIKPKLTETEIEASKSMDE"
     gene            complement(11064..11984)
                     /gene="pfkB"
                     /locus_tag="ACGEXS_001300"
     CDS             complement(11064..11984)
                     /gene="pfkB"
                     /locus_tag="ACGEXS_001300"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFF8012318.1"
                     /translation="MIYTVTFNPSIDYVIFTNDFKIDGLNRATATYKFAGGKGINVSR
                     VLKTLDVESTALGFAGGFPGKFIIDTLNNSAIQSNFIEVDEDTRINVKLKTGQETEIN
                     APGPHITSTQFEQLLQQIKNTTSEDIVIVAGSVPSSIPSDAYAQIAQITAQTGAKLVV
                     DAEKELAESVLPYHPLFIKPNKDELEVMFNTTVNSDTDVIKYGRLLVDKGAQSVIVSL
                     GGDGAIYIDKEISIKAVNPQGKVVNTVGSGDSTVAGMVAGIASGLTIEKAFQQAVACG
                     TATAFDEDLATRDAIEKIKSQVTISVLDGE"
     gene            complement(11981..12745)
                     /locus_tag="ACGEXS_001301"
     CDS             complement(11981..12745)
                     /locus_tag="ACGEXS_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFF8012319.1"
                     /translation="MMIITEKRHELILEELSHKDFLTLQELIDRTGCSASTIRRDLSK
                     LQQLGKLQRVHGGAMLKENRMVEANLTEKLATNLDEKKMIAKIAANQINDNECLFIDA
                     GSSTLELIKYIQAKDIIVVTNGLTHVEALLKKGIKTIMLGGQVKENTLATIGSSAMEI
                     LRRYCFDKAFIGMNGLDIELGLTTPDEQEALVKQTAMSLANQSFVLIDHSKFNKVYFA
                     RVPLLESTTIITSEKALNQESLKEYQQKYHFIGGTL"
     gene            complement(12995..13477)
                     /gene="ybaK"
                     /locus_tag="ACGEXS_001302"
     CDS             complement(12995..13477)
                     /gene="ybaK"
                     /locus_tag="ACGEXS_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFF8012320.1"
                     /translation="MAKNKKTNAMRMLDRAKIKYEVHSFEVPEEHLSGQEVAELIQAN
                     VKTVFKTLVLENTKHEHFVFVIPVSETLDMKKAAALVGEKKLQLMPLDNLKNVTGYIR
                     GGCSPVGMKTLFPTVVDKSCENYSHISVSGGLRTMQITIAVEDLITITKGKIGAVIHE
                     "
     gene            complement(13651..14103)
                     /locus_tag="ACGEXS_001303"
     CDS             complement(13651..14103)
                     /locus_tag="ACGEXS_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012321.1"
                     /translation="MTVKNLFLGFVAVILTVCLIGLLILATNEDALAKVHKTINTLNA
                     INVSTEDTYKKKMDILNIHTAKASEVNENVKKQNHFKHRVNANKSNSFNEQECQVIAD
                     RYADKHINDNYGLERISKTNHGYNYVYSNDNSTSKQHVSISNQGIITK"
BASE COUNT         4659 a         2630 c         1961 g         4984 t
ORIGIN      
        1 taactggatt caatttatat aggcatcaaa tgcaacattt atcattaagt gcaatgttgt
       61 tatctattac gataaacgcc tttataaata cattcataat tgataaatat aactttatta
      121 ctctgtgtac tataacgatg tttatcattt ggacgatact acagttttac attgataaaa
      181 aattaaaacc tgtatacatt acagaccaaa aatttattgc tatcatatta acgattgttg
      241 tcagtttaac gcaacgtgtt accgactttt caagtacaca atcaatatat atgtctattc
      301 catttttagc ccctgccatt ttcatcattg gtggtattat gttgtttatt tcaacattta
      361 atagcttaga tgaaactgct gaaaataata acaaaataaa gaaactaatg attaaaggac
      421 ttatcattat taacatttca tttatcgtta tgatggtttt aacaccatat tggtacttgt
      481 atttaatcgt ctatcttatt ttcttgttgt ttttattgtg gcaaaaggtt tataaatttt
      541 aataccaaaa ctattaaaca cttctgatat tcttagttca aaatatcaga agtgttttat
      601 agtgttatct agttcagata aatatttcct tacttaaaaa aacgccctcc tcttattttg
      661 acccctattt atttaaatca gacaattatt ttcattttca aattattctt tcttcaatat
      721 tagttaagcg atatttaaac gaagttaaga attagttaat ggcatattat ttgccttcat
      781 tttaaactta acttatcaaa ttgaagaaat gaggagttag catgaataca aaatattttt
      841 tagcagctgg tgctgttatc acaacattag ctttaggtgc ttgtggtaat tctaattcac
      901 aagatcaagg taacaaaact gaacaaaaaa caaagtcaga agatagcaat gttaaaactg
      961 ataaaacaaa acacctaaca ggtacattca gttctaaaaa cggtgaaact gttgaaggta
     1021 aagctgagat taaaaatggt aaattaatgc ttactaacta caaatcatca aaaggtccag
     1081 atttatacgt ctacctaaca aaaaatggcg acattaaaaa cggtaaagaa atcgcaatgg
     1141 ttgactacga taaagaaaaa caaacatttg atcttaaaaa tgtagattta agcaaatatg
     1201 atgaagtaac aatctattgt aaaaaagctc acgtcatctt cggcggcgct aaattaaaat
     1261 aatcaatttc tgagttaaac ttttatttac aacatactat tactatacat tacaaatttt
     1321 taaaatatac attacactca ttactcaatg gaaagcgtat gatttcacag cccccctagc
     1381 ttgtagaaat catactttcc ttttttcaat atatatacaa ctattaaatc ccataagatt
     1441 gcagagcaca taagtaaatt tttttagagc ttgaggtttg tttagcttaa gcaacccatg
     1501 agctcaaaca cttcctgttc acataacact acaaatcgca ttatgttgct taatcttatg
     1561 tttatataaa ttacacacaa taaatagaaa gaatgtgaac atcatgaata aattattgct
     1621 actcgttaca tttatcattc gtgtgggttc aggtattgtt atgttaatgc aaggctacga
     1681 aaaattaacg ggcggattta cgctgaaagg tttagtacca gtcatcgcta acaatactga
     1741 ttcaccagag tggtataagt ggtttttcgc aaatatagtt gcacatacga cgtcattatt
     1801 tgatattgtt gtcccactcg gagagattgc aattggatta ggtttaattt ttggagtttt
     1861 tgcatatgct gctagtttct ttggagcctt tgttatgata aattatatct tagcagatat
     1921 gatatttacg tatcctcttc aattaacttt ctttatcctt ttactaatga gtcactcatt
     1981 gttaaaacag atttcactta aagaaatcat taattacttt agaggtcgta agaacagagg
     2041 tgaaaaaata gatgacccac ttactgatcg tggatgatga acaagacatt gtagacattt
     2101 gtcaaaccta ttttgaatat gaaggttaca aagtaacaac gacaactagc ggtaaagaag
     2161 caatttcttt actatcaaat gatattgata tcatggtact tgatatcatg atgccagaag
     2221 ttaatggtta cgacattgtc aaagaaatga aaaggcaaaa attagatatc ccctttatct
     2281 atttaactgc caaaacacaa gaacatgata ccatttacgc cttaacttta ggtgcagatg
     2341 actatgtcaa aaaaccattt agtccaaggg aactcgtttt acgtattaat aatttactta
     2401 caagaatgaa gaaataccat catcaaccag ttgaacaact gtcgtttgat gaattaacac
     2461 ttattaactt aagtaaagtt gtgactgtaa atggtcacga agtccctatg cgtattaagg
     2521 aatttgagtt attgtggtat ttagcttcta gagaaaatga agttatttct aaatcagaat
     2581 tacttgaaaa agtttgggga tatgactatt acgaagatgc taataccgtg aatgtccata
     2641 tacaccgtat tagagaaaaa ttagaaaaag agagctttac aacatatacc atcacaactg
     2701 tatggggatt aggatataaa tttgaaagga gccgataatg gtgttatcaa ttagaagtca
     2761 aatcattatt ggcgtcgtat cgagtatact attaacttca actattttag caattgcata
     2821 tattttaatg tggtttaacg gccatatgac actaactttg accttaacga caataattac
     2881 aagctgttta accttattaa tatgtagtat ttttattaat ccacttatac aaaaaattaa
     2941 gcagtttaat ataaaaacta agcaatttgc taacggaaat tacgcaagca atgataaaac
     3001 gtttaattca ccaaaagaaa tttatgaatt aaatcaatct tttaataaaa tggcttctga
     3061 aattacgcaa caaatgaatc aaattaaatc cgaacaacaa gaaaaaacag aactgattca
     3121 aaacttagcc catgatttaa aaacaccttt agcaagcatt atttcatatt ctgaaggact
     3181 acgtgatggt ataatcacta aggatcatga gattaaagag tcatacgaca tattaattaa
     3241 acaagcaaac agattatcaa cattatttga tgatatgact catattatca ctttaaatac
     3301 aggtaaaaca tatcccccag aattaataca actagaccaa ttacttgtat caatattgca
     3361 accatatgag caacgtatca aacatgaaaa ccgcacatta gaagtgaatt tctgtaacga
     3421 aattgatgca ttttatcaat atcgaacgcc acttgagcgt attttaacaa acttacttga
     3481 taatgcgcta aaattttcaa atgttggtag tcgcattgat attaatatta gtgaaaacga
     3541 agatcaagat actatcgaca ttgctattag cgatgaaggt attggcatta taccagaact
     3601 acaagaacgt atattcgaac gtacattcag agtagaaaac tctcgtaata caaaaacggg
     3661 tggttctgga ttaggcttat atatagctaa tgaactcgcg caacaaaata acgcaaaaat
     3721 cagtgtaagc agtgatatag atgtaggaac tacgatgact gtaacattac acaaattaga
     3781 cattacgtca taatccgatt tatttataaa ataaaatgca aagactaaaa agaagctccc
     3841 attaatgagg gcttcttttt ttgtttattt agaataaact ttatgggtat ccttctcatc
     3901 attttcaaga cttgaaagat ttgtagcttg aataatatat ttaggacgtg ccttaacttc
     3961 ataatatatc ctgccaatat attcacctac aacaccaatt gaaattaact gtatgccgcc
     4021 taataataaa atagctgcaa tcgttgaaaa atatcccgga atattaacac cagatatcat
     4081 aatattgatg aataaataga taatatataa cacgcttatt gaaaagataa acaagccaag
     4141 ataaatcatc attctcaaag gtttactatt aaaggaaatc aatccatcaa taccataatt
     4201 aaataacttt ttaaaggacc acttagattc ccctttttgt ctctcaacat tttgatacgt
     4261 aaacacttta gtattatagc ctatccattc aaataaccct tttgaaaatc gattatattc
     4321 ttcaagtgat gcaatggatt taacagctct ttggcttaaa agtctaaaat caccaacacc
     4381 atcatcaaat tgtacttctt ctacaaagca attaactaac ttataataca aatggcttaa
     4441 tgttttgcga ctaaaatttt caccacttct atcacgcttt gcaatcactt gatcatagcc
     4501 ttccataaaa ccttcaacca ttttcggaat atattcaggt ggatgttgca aatcacaatc
     4561 tatcatgatg actgcatcaa attcagtact atgctggtaa cctgcaatca tagctgcttc
     4621 ttttccaaaa tttctactaa aagaaataaa gctgacatgc ctatctattg tggcaagatg
     4681 ttgcatttca tctatagtgg tatccgtact accatcatct atgaaaagca tattataatc
     4741 atagcctttc acactactat cttgtgaaag tatttcagtt aattgttgat gtgtttgtgt
     4801 aatgacttcc ccttcattaa aacaaggaat gacgactcta attttcatct atgtccatcc
     4861 tttttattta aaagattttg aatgtacctg agtattgttt tatcaaatag taaaaaccaa
     4921 ctcatttatt ttaatgtaca cattaaaagg cattcataaa cgtaaaattt aacatccagt
     4981 tattttaaat ccccaatttt cacatcatct ggatttttac cttgtcttgc atttctattt
     5041 aaagcatcaa tttcagctac ttctgttaat tctaaattaa aatctaaaat atctatattt
     5101 tcttgaatgc gtttaggtgt ctgagatttt ggaataataa ttctattgtg tgctaaatgc
     5161 caacgtaata caacttgtgc tggtgtttta tgatattttt cagcaatttt aacaatgaca
     5221 gggtcgtcta gtagtcctct atttctcatc aaaggcatcc atgcagtcac tttaatatcg
     5281 tgacgatcac aaaattcttg taattcttgt tggttaaaat atggatgtac ctcaatttga
     5341 ttcaccattg gtttgatact tgattgagcc attaattttt ctagatgatg aacattaaaa
     5401 ttacatacac ctattgcttt taccttacct tgctcgtaaa gttcttccat agctttatat
     5461 gtttctaaaa atagtccatc tgcttcacaa ggccaatgta ttagaaataa atcaagataa
     5521 tcagtttgta aattttcaat cgatttgttg aaatattcga atgttttctc ataaccttga
     5581 tagtcattcc ataacttcgt tgttataaac aaatcttctc tatcgacgcc attatccttt
     5641 aatgctcgtc ctagtgaagc ctcattatca taaaagtatg ctgtatcaaa cgctctatag
     5701 cctgcgtcaa ttgcagcatt tacaacttta gtcatatctt cgtcagagat tttataaaca
     5761 cctaacccaa ctgaaggcat cgggtatcca ttatttaata tttgtatctc attcaacatc
     5821 tttatatctc tccaatctat gtatctttta tatctttaca ttaccctaaa tttttcaaca
     5881 aactcaatta atacgaatta tcgctttcaa taaaaattat tcattaaatc attaaagata
     5941 ttgagttcca atactatttt cacttttcat taattttagt caaaaaaata accaaccaaa
     6001 aatgaattaa atcattctta gttggtttat atattaatat ctattctgat ttttcatctt
     6061 catcagactg tccgatagta ggtctcgctt cattaaattc atagtttaat atcacccaaa
     6121 taatttggtg gttatcgatt tctgaaacaa cccagcgatc ataagttgta tccacgtaat
     6181 catctttttg taaattggta ttacgagatt gtaaccatcc acctatcgta tcaatatcct
     6241 cagagtcatc aaattctata ccgaactctt cagttaaatc atccaatagt actctgccat
     6301 ttacttggaa tgtcttatta tcaattttaa cgatatcatt cacttcatca tcatcaaatt
     6361 catcacgaat ttctccaacg atttcttcta aaatatcttc catcgttaaa atacctgccg
     6421 ttccaccata ttcatctata ataagactca tatgtacatg ttcacgttgc attctaatta
     6481 atgcatcact gatacgtgtt gtctctgaaa tcattggcaa ctcatgtata tagtttgcta
     6541 ttttaatcgt ttttccagaa gcgtattcag ttaaaaattc tttgacgtta ataaatcctt
     6601 taatgtggtc tttatcacca tcatcagtaa ttggataacg cgtaaattga tgttctttta
     6661 ttgtttctag taattcgtct acattaaaag gttcatttag tgtaatcatt tgagttctag
     6721 gtaccattat atcttttgca tgtctttcat cgaatgaaaa gatattttgc atatatgcca
     6781 attcagtttg gttgatttct ccaccattat aactattgtt aataataatt ttgatttctt
     6841 cttctgacat tgcatcagtt tgggcatcag gatttacacc aaacattcta ataataacac
     6901 gtgcagaacc attcatcagc caaatcaatg gtttcataat gttaccgaaa tagaacaatg
     6961 gtcttgcata tactaaagca agcttttcag tatgttgaat agctatagat ttaggcgcta
     7021 attcaccaag tactacatgc aaatacgtaa cgattataaa tgacactgca aacgaaatcg
     7081 tcgtcgttaa tgcagttggt aaattgattg cttcaaatat tgggtgtaat agcttttcaa
     7141 acgttggttc accaagccaa cctaacccta aagatgttac tgttatacct aactgacaag
     7201 cagaaagata ataatctaga ttagcaatca tcttttttac tattttagca ggtttatttc
     7261 cttcatctgc tagctgttca attcttgttg ctctaatttt tactaatgca aattctgaac
     7321 caacaaatac agtggttaat gcaattaata gaataaatat aatcaaacta attatggtcg
     7381 aagtttccaa ttaattccct atttctaggg attcacctcc atgttatatg tgtcactcat
     7441 gggtaacacg cattcaaatt tatcactatg acttaaatta caactacatg ttatcgcctt
     7501 cccatttaga cacccccaga aaaaaatgtt attgcgtcta ttttatcata atataaagtg
     7561 cttatgttaa cagattaaat ctattgcata catttaattc tgattattta ttagctaaat
     7621 gtgtgaacct tgccttgttt tattgtagat tttacattca tatcatcatc tagaataaca
     7681 agatctgcat ccttatttac tttaatacta ccttttctat catcgatacc taatgcaatg
     7741 gcttgattta aacttgttac tcgccataaa tgatctaatg tatcacctgt aaatgatatt
     7801 aagttacgta acccatgatt catttttaaa atactaccag caagcgcacc atttgcaaga
     7861 cgtgcttgtt gcgattgaac agttactttt tgtccaccca aatcatattc tccttcaggc
     7921 ataccttttg cacgcattgc atcggtaatt aaataaaaac gttcattacc tttcatacgg
     7981 taagcaattg caaccgatgc cggatgagaa tgagtgccat caacaatcat ttcggtatgt
     8041 agagcatcat tcaaccatgc tgctccaaaa acacctggtt ctctatgttg gaatggcgtc
     8101 gctgcattat ataaatgcgt gacatgttta gctcctcgct caacagcttc aactgcttct
     8161 tcgtatgttg ctactgtatg accaattgaa aaaataatgt catctttata cgtttcaagc
     8221 gcttcttttg caccttcaac ttcaggtgca aacgtcataa tctttattaa tccgttagca
     8281 gtctcttgaa aatgtttaat tttatcgata aatgggcgta caacgtattg cggatgttga
     8341 gcaccaactt tattttcaga tataaatggt ccttctaaat gtatacctac aatttccgct
     8401 gcattgtgaa catcttgctc cgcttcatat ttagcaatat ttgtaagtgc attatctatt
     8461 ttatcagtcg attgcgtcat tgtagtggcc aagtatgatg tcgtcccttc agacaacaaa
     8521 ttttcggata gatattttaa gccatcgtat gacccatcca ttgcatcttg accataacca
     8581 ccatgaatat gtatatcaat aaaacctggt aatacatgat gacctttagc atcaatcact
     8641 tgaattttat ttgtcgtatc attatcaatt gctgctttat catccacttc tccaattgca
     8701 acaatctgtc catctttcac atgaatgtaa ccattatcga ttttgccatc ttcagtataa
     8761 actttgccgt tatatataat taattctgac acgtacaaac ccttctttat ttataaattt
     8821 tcactttaag acactcatat tttactatat ctgcttcgtt tggcataact ttttgcaaaa
     8881 tattaaaaaa gtctatacat ttgtggtgtc tatttcttca ttcatttatc taaaaataaa
     8941 aacactttca tatcaattca taaaaactga attaatagaa agtgttttta cagctttgta
     9001 taagctaccg ttaacacgat atataaaact aacatatatt cattgaacat acaacttaat
     9061 atgaagctct ttgctaacaa tcattttaca tcattaaaac tactcgtcca ttgattttga
     9121 agcttcgatt tctgtttcag ttaactttgg tttgattaaa ccgtaaatta atgctgaaac
     9181 taatgtgcca actagaagtg caataagagt ttgaagtaag tgtgcaccat cagtaccaac
     9241 aattacaata ataccaccat gtggcgcagt aattcgtgaa cctaagccta aagcaattgc
     9301 gccacctata cctgaaccaa tcatcattga aggaataaca cgtaatggat cggcagctgc
     9361 aaatggaatc gcaccttctg taataaatga catacccatc acatagttag ggataattga
     9421 accacgttgt tcttttgtaa atttacgtct aaaaattaac atcgctgtcg caattgctaa
     9481 cggtggaatc ataccaccaa tcattgcagc tgtaattggt gctgcattac cttcaatcaa
     9541 cgcacctgtt gcaaaaacat atgccgcttt gttgaatgga ccgcccatat caatcgccat
     9601 catagcgcca ataactaaac ctaataatac aatattagaa cctgataaat tgtttaatcc
     9661 atctaataac aaatgattta accaagatgc tggtggatta aaggcataaa tcatcaataa
     9721 gcctgtagcc gtcactgtta atagtggata aattaatgtt ggctttaatc cctctaatgc
     9781 ttgtggtaac ttacgtgtca tggctttaac accttgtgtt aagtaacctg ctaagaaacc
     9841 tgcaataata ccaccaataa atcctgaacc acctgaaatt gctaacatac cacctacaag
     9901 acctgaagcg aaaccaggtt tatccgcaat actacgtgca atgaatccag ataaaattgg
     9961 aatgattaac gcgaatgcac ttttactacc aatgttccaa agctgctctg caaacgcatt
    10021 gtactctgag ctttttggat taaatgaatt tgctccaaat aaaaatacaa ttgccattaa
    10081 aataccacca gatattacaa gtggcaacat gttagaaaca ccgttcatta agtgtttata
    10141 gaatgcttta cctgggctta atttttcttg actgtcattt gagttacctt ttctttgacc
    10201 atcacgggca acaaaaggtt tacgacttgt atctaatgct ttattaatta attcttctgg
    10261 gcgtttaata ccatctgcta ctggtacttc tacgacattt ttaccatcga agcgatccgt
    10321 ctcaacatga acatcagcag caacaatgat acctgttgca ttttcaatat cttgttcagt
    10381 taaatggttt ttaatgccgc ttgaaccatt cgtttctact ttaattttaa tacccatttt
    10441 atccgcttgc tttttcaatg catcacgtgc catatatgtg tgtgcaatac ctgttggaca
    10501 agcagttact gctaacacat atggctcatt agattgttta gatgattgtt ctgcagttgc
    10561 aacttgttgt gcttcagctt ctgcctcttc ttcttttgtc acttcatcat cagcctcatc
    10621 tatgatcgct agtacttctt caggtgatga agcatgtaat aatttctcac gtacattttc
    10681 atccattaaa ataccagaca acttagctaa agcatctaga tgtgtttggg cgccaccttc
    10741 tggcgctgca atcataaaga ataagtgtgc tggttgcata tccaaacttt gataatctac
    10801 gcctgcttta gatttaccaa acgcaatagc tggtgactta actgcggcca ctttggcatg
    10861 tggaatggca ataccttcgc cgataccagt tgtactttgt gattctcgat tgtgaatcgc
    10921 ttccttaaat gacgcgacat cacttaattt acctgctttg tctaattgat ttactaactc
    10981 atcaataaca ccatttttgt catttgccat taaatccatt gctattgtat cttttgttaa
    11041 taactctgtt actctcatta ttttcactcc ccatcaagta cgctaatcgt aacttgtgat
    11101 tttatttttt ctatagcgtc ccgtgttgct aagtcctcat caaatgccgt ggcagtaccg
    11161 catgcgactg cttgttggaa tgctttttca atcgttaaac ctgaagcaat tccagccacc
    11221 atgcctgcaa ctgtactatc accagagcca actgtattaa ccactttccc ttgtggatta
    11281 actgctttaa tactgatttc tttatcaata taaatagcac catcaccgcc aagcgagaca
    11341 ataacagatt gcgcaccttt atcaactaac aaacgaccat atttaataac atctgtgtct
    11401 gagttcactg ttgtattaaa catcacttct aattcatctt tattaggttt aataaatagt
    11461 ggatgatatg gtaaaacgct ttcagccaat tctttttcag cgtcgactac taatttagca
    11521 cctgtctgtg ctgtaatttg tgcaatttgc gcatacgcat cgcttggaat actacttggt
    11581 acacttccag caacaataac tatatcttcg cttgttgtat ttttaatttg ttgtaacagt
    11641 tgttcaaatt gtgttgacgt tatatgagga cccggtgcat tgatttctgt ttcttgtcct
    11701 gtttttaatt tcacattaat acgtgtatct tcatcaactt caataaaatt cgattgaatt
    11761 gcactgttat ttaatgtatc tataatgaat ttcccaggaa atccacctgc aaatcccaag
    11821 gcagttgact caacatccaa tgtctttaag acgcgcgaga cattaatacc tttcccccca
    11881 gcgaatttat atgttgctgt tgctctgttc aaaccatcaa ttttaaaatc attcgtaaaa
    11941 atgacatagt caattgaagg attgaaagtc actgtataaa tcataaagtc cctcctataa
    12001 agtgatactt ttgttggtat tcttttaacg attcttgatt taatgctttt tcagatgtga
    12061 tgattgtcgt actttctagc aaaggtacac gagcaaaata tactttatta aacttagaat
    12121 gatctataag tacaaatgat tgattggcta atgacattgc tgtttgttta actaatgcct
    12181 cttgctcatc gggagtagtt aatccaagtt caatatctaa tccattcatc ccgataaaag
    12241 ctttatcgaa acaatatcgt cttaatatct ccatagcact agaaccaatc gtagcaagtg
    12301 tattttcttt aacttgacca cctagcataa ttgttttaat acctttttta agtaaagctt
    12361 ctacatgtgt taaaccattg gttaccacaa tgatatcttt cgcttgaata tatttaatta
    12421 gctccaatgt agatgaacca gcatcgataa ataagcattc attatcgttg atttgattag
    12481 ctgctatttt agcaatcatt ttcttttcat caagattcgt tgctaatttt tcagttaaat
    12541 tcgcctcaac catacgattt tcttttaaca ttgcaccacc atgcacacgt tgcaatttcc
    12601 ctaattgttg tagtttagat aaatctcttc gtattgttga agcactgcaa ccagttcgat
    12661 ctattaattc ttgtaaagtc aaaaaatctt tgtgcgaaag ttcttctaat attaactcgt
    12721 gtcttttttc agtaattatc atcatcaccc catctcactt tgtattataa tgagtacgct
    12781 ttcacttttc aatcataaat taccaaaaac aatcacaaac actcaaattg tgacgataaa
    12841 attacatttt tatgttcaaa tactcaatta taatgactgt ttatgatcgt aaaatgctaa
    12901 ttcaatcaaa aaggagtgtg gcagaaatga attttataaa aatttcatct ctgtcacact
    12961 cctaatcttg ccccatactt tttgttgtta ttaatcattc atggataact gctccaattt
    13021 tgcctttagt tattgtaatc aaatcctcaa cagctattgt gatttgcatt gttcgaagcc
    13081 caccactcac actgatatga ctataatttt cacacgattt gtcaacgact gttggaaaca
    13141 atgttttcat accaacaggc gaacacccac cacgaatgta tcccgttaca tttttcaaat
    13201 tatctaaagg cataagctgc aatttcttct ctccaaccaa agcagctgcc tttttcatat
    13261 ctaaagtttc acttactggg ataacaaata caaaatgttc atgttttgta ttttctagaa
    13321 caagcgtttt aaatactgtt ttaacatttg cttgtatgag ttctgcgact tcttgaccag
    13381 ataaatgttc ttctggtacc tcaaagctat gaacttcgta tttaattttt gcacgatcaa
    13441 gcatacgcat cgcgttcgtt ttcttatttt tagccatttc aacacctcat ttatacttct
    13501 tcagaactat attaacattt taaaatacat tacggattgg ttgattttga cataacattt
    13561 acatcatttt ataagttgaa ttcacctttt taaattaaat tattgcaaaa taaaaacagt
    13621 atatccaatt agaatacact gttccatcta ttatttcgtt attatgcctt gatttgaaat
    13681 acttacatgt tgcttactag ttgaattatc attggaatac acataattat atccatgatt
    13741 tgtcttagaa attctttcta aaccataatt atcattgata tgcttatctg cataacgatc
    13801 agcaataact tggcactctt gttcgttaaa agaatttgat ttatttgcat tcacacgatg
    13861 tttaaaatga ttttgctttt tcacattttc attcacttca gatgctttag cagtatgaat
    13921 attgagaata tccattttct ttttataagt atcttcagtt gatacattta tcgcgttaag
    13981 cgtattaatt gttttatgta ccttagcaag cgcatcttca tttgttgcta atattaataa
    14041 accaattaaa caaacggtta atattacagc aacaaagcct aaaaataaat tttttactgt
    14101 catcccctta cccacatttc cttactcatt ttttatcatc ttaacacatt aacgttaatg
    14161 aattaggtat gtggattgca atttaaaaag aatgtaatat ttgtaacatg tttattactc
    14221 aaaatcattt tttg
//
LOCUS       DAXWFK010000056        14223 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.056, whole genome shotgun sequence.
ACCESSION   DAXWFK010000056 DAXWFK010000000
VERSION     DAXWFK010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14223
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.056"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            70..684
                     /locus_tag="ACGEXS_001304"
     CDS             70..684
                     /locus_tag="ACGEXS_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012322.1"
                     /translation="MGNLDFDHEGYRKLLPLKDFKHFIKHSDSKTRNHFIGMAFNNKT
                     DVFSRNFSNKMINIFPKNEFYIHIQQFPENDSPSYMVYNTDITAFIQFRLVGRSENKY
                     LENIIRDYGVLIEVEALHSFKKGMGAMLVSQLEKLSDKLFIPIYLYDTNLKDELYYQD
                     LGFFDTTKKGNHGEPLLVYKPKNLETNKKEKPIKSLFKKVFNIN"
     gene            753..2024
                     /locus_tag="ACGEXS_001305"
     CDS             753..2024
                     /locus_tag="ACGEXS_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012323.1"
                     /translation="MDYTNQKRKYIIFGIVLAITLLIVGIAYYLISLNNAKGQVKEFE
                     KLVQHNDYNSIAKKLSNKEETLSENDAKNFVLFVKSKENQEQYNEQIQKIKSNIDKNK
                     DNNTVYGSITDQYGNNLITIKKNGKTFFFIDKLEFKPKLTSVYVKEFNNTGIYKYQQE
                     NAKKVIAESNQTTELGKFFIGKYSIKTDKTIEDSIHNGTLTGKIDIDLSNRGNNNKIY
                     ADEDFNQSWFKVKLNNDSLLEKNSYKLLIDDKDVDYNSKKTYGKYYNPTELSVYAIGK
                     LEGKEFKTNRINIRRNYDNKPQNLKLSFKESQIRDYESKSKKVKEKAKSYIKEYTKEL
                     NKAYKKKDYKYISDYVKNNSQLEKQLKKQVKSKSKVKFKDVKINNVKRDKNKIYIELS
                     KTNNGNVFQTKYTLDYDNVSNEFLVEDSHDN"
     gene            complement(2066..2482)
                     /locus_tag="ACGEXS_001306"
     CDS             complement(2066..2482)
                     /locus_tag="ACGEXS_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YolD-like family protein"
                     /protein_id="HFF8012324.1"
                     /translation="MKIINPNMPEPYKYETDYRNIPREYLNPHIPKGRGIVKWNAFKT
                     IPQQYEILEQHIEDQNKINMPLLSDDQLQEINEKVSSKISKNILTDLDYWKDGYIYSI
                     QCYINKIDEFNGFMIITNSNSNNKLKIPLSSIVSVQ"
     gene            2777..3334
                     /locus_tag="ACGEXS_001307"
     CDS             2777..3334
                     /locus_tag="ACGEXS_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8012325.1"
                     /translation="MANIGYARVSTRDQSLDLQIDALSNFGCEKIFSEKVSGRKVNRT
                     ELDKCLDYLREGDTLVIYKLDRLGRTTKQLIDLAQWLENNNIELQIIDMNINTKDPMG
                     KMFFTMMSAFAELEANLLSERTKKGLESARARGNMGGRKAMPQEKKEYIKHLYDTKEY
                     TGQEIAKKTNVSRDTVYRILREFKK"
     gene            3616..4395
                     /locus_tag="ACGEXS_001308"
     CDS             3616..4395
                     /locus_tag="ACGEXS_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012326.1"
                     /translation="MEVVRLNQNLFNKLRGNEISSNKNGSRPYYYSFKRNNNRVCIPF
                     RTNAQKVPNKYKINLGGEQPDKPNSAIDLTKSIVISNDEYLNNRSKAKIPQNVNNFLK
                     QQAPAIEQKYDTMSNDYIKAKASLSKIPLVKYSTMQYFHKELNIQDSIDNQQTKNAIN
                     ELISNGKSNKYNKLQSSLPNEKLNLLDDYETLYEFKSLTDYPAKINSNDIDNPFLEVE
                     KNNKHFTLSALTIKNEPEKHVKDFLNYDIENEKNKDIDLDL"
     gene            4529..6145
                     /locus_tag="ACGEXS_001309"
     CDS             4529..6145
                     /locus_tag="ACGEXS_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012327.1"
                     /translation="MRFTPRGILNNTDYELNSEFYYVPASDKQMNDQRDMTNKSIVGN
                     LVYRNNFYNEVYTQPVYDLDDSNEKYQTPHIISIDDSEFDYNLTNLDDYFTKDTANKG
                     YEKLVNAIKSEEITYFDSDINEVDDFTQQDEYSNDWYTNTFVDLVLSMKGYSYHVEPN
                     RESVIEDSHIITENDITYNVSYNPEKHHLYINDFPAIPIEENDNIRLLNDNLTQDMEK
                     TYDNINYNFSYNDFKTLEDDSYIYIDLTDYKDNEGKFVKNLIEDIPPNEIIKDRLKQN
                     INLFDERANYNALAVYRRDLEDFSDRNNLDSLDKVKDLKTFYDTEINIKGLNINNDIE
                     KKALNNQLAFNLYNEPLNNMFRDNNVLYEVYEKEIYDEKIPLSIEDRKEVKTYLDDAR
                     NNFSNNFYEYTTNKSSILAENSLTVLDKAKYDLEFAPVSNHLYKERENFYEGMKQATL
                     PLHNITKEQYKERENDIEIIGHFKNYINTELHGTKSSEFKPNFVNDSLENETNSIAFE
                     DIINGSEETHSNIINSIKNELKQERDNLEM"
     gene            6156..6344
                     /locus_tag="ACGEXS_001310"
     CDS             6156..6344
                     /locus_tag="ACGEXS_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000921494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012328.1"
                     /translation="MLKTYTEIIGSELPIYITKSETSELLFSEELDKNLKYEVAGYYL
                     YNAKKDLYKELYKPISEV"
     gene            6348..8072
                     /locus_tag="ACGEXS_001311"
     CDS             6348..8072
                     /locus_tag="ACGEXS_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFF8012329.1"
                     /translation="MAYSKGNYKKKKIDSIIEELNNKLDDFKNNDKTYKEFLDTTAKF
                     HNYSINNIILIAGQNPEATAVAGYKAWKNKFDRQVQKGAKSMNIIAPIVQKRNVEIKD
                     DNGNIVRDNNGNPKTERKPVITGYRAHNVFDVADTKGKPLITAKDLIKTEFENSNDYK
                     DLYNEFKDYINEELTPSVEEKHFLDDPTLSNGAKGYYSPKSDEIVISDDLSYDMRFKT
                     LIHEYAHSQLHNNDIGKTQISEHSRSLKEIEAESSAYVVANYYGLDTSDYSLGYLSGW
                     GHNISDDELKAHIKNIHSFAKTTIEEINSLPEFSQYIDKKLESEMNKDVYKDLSTMID
                     TNLKNGFDKITIIKGNLVNDYGLNEISENSYENDDFKVNIDYKGFNTNNSQDQAKIEL
                     INKHDDSLNRDYNFTQTYNRNVINNTTTIFVQDDDNEDKNKKYIHERDIDGNILEEKH
                     KLSPQDEIVSFENFVNKSVEENGLKDTLRQFVSNGVLMGYELNVDNIDNTNETYFSMN
                     KNQQNGQRSNMTAKLEHDTNENVYIDFKIKNSAGIKNISYTESNEEFNKDEQNKEIEN
                     KQEEEIDI"
     gene            complement(8113..10110)
                     /locus_tag="ACGEXS_001312"
     CDS             complement(8113..10110)
                     /locus_tag="ACGEXS_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HFF8012330.1"
                     /translation="MAMYHFQNKFVSKANGQSATAKSAYNSASRIKDFKENEFKDYSN
                     KQCDYSEILLPNNADDKFKDREYLWNKVHDVENRKNSQVAREIIIGLPNEFDPNSNIE
                     LAKEFAESLSNEGMIVDLNIHKINEENPHAHLLCTLRGLDKNNEFEPKRKGNDYIRDW
                     NTKEKHNEWRKRWENVQNKHLEKNGFSVRVSADSYKNQNIDLEPTKKEGWKARKFEDE
                     TGKKSSISKHNESIKKRNQQKIDKMFNEVDDMKSHKLNAFSYMNKSDSTTLKNMAKDL
                     KIYVTPINMYKENERLYDLKQKTSLITDDEDRLNKIEDIEDRQKKLESINEVFEKQAG
                     IFFDKNYPDQSLNYSDDEKIFITRTILNDRDVLPANNELEDIVKEKRIKEAQISLNTV
                     LGNRDISLESIEKESNFFADKLSNILEKNNLSFDDVLENKHEGMEDSLKIDYYTNKLE
                     VFRNAENILEDYYDVQIKELFTDDEDYKAFNEVTDIKEKQQLIDFKTYHGTENTIEML
                     ETGNFIPKYSDEDRKYITEQVKLLQEKEFKPNKNQHDKFVFGAIQKKLLSEYDFDYSD
                     NNDLKHLYQESNEVGDEISKDNIEEFYEGNDILVDKETYNNYNRKQQAYGLINAGLDS
                     MIFNFNEIFRERMPKYINHQYKGKNHSKQRHELKNKRGMHL"
     gene            10314..10586
                     /locus_tag="ACGEXS_001313"
     CDS             10314..10586
                     /locus_tag="ACGEXS_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012331.1"
                     /translation="MADIQKMLEQKEKLQEKIKEEKKKEYEKFGRWFFNKFKVNRSAD
                     AKKIINKNLLNIEDTKEHNLERNEIIEETKNSNEENKEINEINHNI"
     gene            10690..10923
                     /locus_tag="ACGEXS_001314"
     CDS             10690..10923
                     /locus_tag="ACGEXS_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012332.1"
                     /translation="MILLSTAGENAVHINPIFRFFLYFIMCVPMTVALCTIFLKFISI
                     FILPWKVCLYIGIALGIIVTIFLLVITIGIEMT"
     gene            11186..12226
                     /locus_tag="ACGEXS_001315"
     CDS             11186..12226
                     /locus_tag="ACGEXS_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="parM protein"
                     /protein_id="HFF8012333.1"
                     /translation="MSNVYVMALDFGNGFVKGKINDEKFVIPSRIGRKTNENNQLKGF
                     VDNKLDVSEFIINGNNDEVLLFGNDLDKTTNTGKDTASTNDRYDIKSFKDLVECSIGL
                     LAREVPEEVVNVVIATGMPSNEIGTDKQAKFEKLLNKSRLIEIDGIAKTINVKGVKIV
                     AQPMGTLLDLNMENGKVFKAFTEGKYSVLDFGSGTTIIDTYQNMKRVEEESFVINKGT
                     IDFYKRIASHVSKKSEGASITPRMIEKGLEYKQCKLNQKTVIDFKDEFYKEQDSLIEE
                     VMSNFEITVGNINSIDRIIVTGGGANIHFDSLSHYYSDVFEKADDSQFSNVRGYEKLG
                     ELLKNKVEQESK"
     gene            12229..12558
                     /locus_tag="ACGEXS_001316"
     CDS             12229..12558
                     /locus_tag="ACGEXS_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParR"
                     /protein_id="HFF8012334.1"
                     /translation="MDKKETKHLLKIKKEDYPQIFDFLENVPRGTKTAHIREALRRYI
                     EEIGENPPIPSKEHTAPLKEHTEPIKRNKESFKDAMERKDQLKQAEMPKIEEKDEEMV
                     DISIDDL"
     gene            complement(12562..12822)
                     /locus_tag="ACGEXS_001317"
     CDS             complement(12562..12822)
                     /locus_tag="ACGEXS_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8012335.1"
                     /translation="MNEYRIQKLYRYICLEFKNQRQLIGKRQEEVAFDLSVTAGHLSR
                     IENGKKPRIALHTFLVMSEYYGVDFHKVVKNAEEKMELDEGI"
     gene            13203..14162
                     /locus_tag="ACGEXS_001318"
     CDS             13203..14162
                     /locus_tag="ACGEXS_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002484669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFF8012336.1"
                     /translation="MSKQFFTVEENYKERFYQLPKVFFTNPNYKDLSNDAKIAYAILR
                     DRLQLSIKNNWIDTEGNIYFIYTVADLEVILNCGNKKITKIKKELENVDLLIQKRQGL
                     NKPNLLYLLKPAITKNDIYEIDKAENEVEALQDKEVSKGHVQKCQKDTSRNVKRTRLE
                     MSKGHTNDTDFIDTDFIDTESNDMNNMNDTNQHSNHSNHFSNIHDKESLKYIELQELP
                     ELIKSYINNFSYEEVKSIKSVILKAKKSFNNKYDTFYMLEDIDEELLLVLKRFKGYLV
                     KKQEKVANMEGYLMRSIIAELEEMHSTIMRRKNMENNPLSLFN"
BASE COUNT         5728 a         1765 c         2012 g         4718 t
ORIGIN      
        1 ataaaagata aaataacatc ttggcttgat aaagataaca acaaaaacga ataatatagg
       61 aggggttttt tggggaattt agattttgat cacgaaggat acagaaaact attaccatta
      121 aaagatttca aacattttat aaaacatagc gacagcaaga ctagaaatca ttttattggt
      181 atggctttta ataataaaac agatgtgttt agtcgtaact tctctaataa aatgataaac
      241 atattcccta aaaatgaatt ttatattcat atacaacaat ttccagagaa tgatagccct
      301 tcttatatgg tatacaacac tgatattaca gcatttattc agtttaggtt agttgggcga
      361 tcagaaaata agtatttaga aaacattatc agagattatg gcgtattaat agaagttgag
      421 gccttacatt catttaaaaa aggtatgggg gcaatgcttg taagtcaatt ggaaaagtta
      481 tcagataaac tgtttatacc tatatatctt tatgatacta atttaaaaga tgaattatat
      541 tatcaagact taggattctt tgatactact aaaaaaggga atcatggaga accactttta
      601 gtatataaac ctaaaaatct agaaactaat aaaaaagaaa agcctattaa atctttattc
      661 aagaaagtat tcaatatcaa ttagcctaca gtattaaaga ggtggggtaa ctcatttctt
      721 taatacttat tttattaaca ggggtttaat atatggatta cacaaatcaa aaaagaaaat
      781 acataatatt tggtattgtt cttgctataa cacttctaat tgttggaatt gcttactatt
      841 taatcagttt gaataatgct aaaggacaag ttaaagaatt tgaaaaatta gtacagcata
      901 atgactacaa tagtattgct aaaaagttat ctaataaaga agaaacttta tctgaaaatg
      961 atgctaaaaa ctttgtgtta tttgttaaaa gtaaagaaaa tcaagaacaa tacaatgagc
     1021 aaatacaaaa aattaaaagt aatattgata aaaataaaga caataatacc gtgtatggct
     1081 ctataacaga tcaatacgga aataacctaa taactattaa gaaaaacgga aagactttct
     1141 tttttataga taaattagaa ttcaaaccta aacttaccag cgtttatgtg aaagaattta
     1201 ataacactgg aatttacaaa tatcagcaag aaaatgctaa aaaggtaata gcagaaagca
     1261 atcaaacaac agaattaggc aagttcttta ttggtaaata tagtattaaa acagataaaa
     1321 caattgaaga ttcaattcac aatgggactt tgacaggaaa aatagatata gatttaagca
     1381 atagaggaaa caataataag atatatgcag atgaagattt taaccaaagc tggtttaagg
     1441 ttaagctaaa caatgatagt ttattagaaa aaaatagtta caaactttta attgatgata
     1501 aagatgttga ttataattcc aaaaagacat atggaaagta ttacaatcca actgaattaa
     1561 gtgtatatgc gattggaaaa ttagagggta aagagtttaa aactaacaga ataaatataa
     1621 gacgtaatta tgataataaa cctcaaaatt taaaattaag ttttaaggaa tctcaaataa
     1681 gagattatga gagtaaaagt aaaaaggtta aagaaaaagc taaaagctat attaaagaat
     1741 atacgaaaga gttaaacaaa gcatataaaa agaaagatta taaatatata tctgactacg
     1801 ttaaaaataa ctctcaatta gaaaaacaac taaaaaaaca ggttaagtct aaatcaaaag
     1861 ttaaatttaa agatgttaag ataaacaacg ttaaacgtga taaaaacaaa atatatattg
     1921 agttaagtaa aaccaacaat ggtaatgtgt ttcaaacgaa atatacctta gattacgata
     1981 atgtatcaaa tgaatttcta gtagaagatt cacatgataa ttaaaataat tcaaacacca
     2041 atgacattaa attcattggt gttttttact gtacgctaac aatactactt aaaggtattt
     2101 ttagtttatt gtttgaattg gaattagtga taatcataaa tccattaaat tcatctattt
     2161 tgttaatgta acattgaatc gaatatatat atccgtcttt ccagtaatct aaatcagtta
     2221 atatattttt actaatctta ctacttactt tctcattaat ttcttgtagc tggtcatcag
     2281 acaatagagg catatttatt ttattttgat cttctatgtg ttgttccaat atttcatatt
     2341 gctggggtat cgtcttaaat gcgttccatt taactattcc tctacctttt ggaatatgtg
     2401 gatttaagta ttccctaggt atgtttctat aatcagtttc atatttgtac ggttcgggca
     2461 tattaggatt tattattttc atatcagtaa acacctcaac caaattatag aacatatgtt
     2521 cgtaaaaata aagagtgaaa taagagtatt ataaacgatt gtatttatta cactgataat
     2581 tacttttctt aatagcgtta taatgggttg actggtgtat taaaatcttg tattataatc
     2641 atagtaagat aattaacaaa taacatggca aataatacaa atagattagc tctcatttgc
     2701 attgagaatt aattttatag ataactatac attaaaaaca atatacaacg ctattttaga
     2761 aaggataatt acaattatgg ccaatattgg ttatgctcgt gtatctacca gagatcaaag
     2821 tttagattta caaatagacg ctttgagtaa ttttggttgt gagaaaatat ttagcgaaaa
     2881 agttagtggg cgtaaagtaa atagaactga actagataaa tgtttagatt atttacgtga
     2941 aggcgacact ttagttattt acaagctaga tagattaggc aggactacta agcaactgat
     3001 agatttagcc caatggttag aaaataataa tattgaatta caaattattg atatgaatat
     3061 caatacaaaa gacccaatgg gaaagatgtt ttttacaatg atgagtgctt ttgctgaatt
     3121 agaggctaac ttattaagcg aaagaactaa aaaaggttta gaatcagcta gagcaagagg
     3181 taacatggga ggcagaaaag caatgccaca agaaaaaaaa gaatacatta aacatttata
     3241 cgataccaaa gaatatacag gacaagaaat tgctaaaaaa acgaatgtaa gtagagatac
     3301 tgtatatcgc attttacgag agtttaaaaa gtaatcctaa acattgcata acatgacagg
     3361 tattgatata atataagtaa gaaaagaatc ggcgttgtgg cgcagatata aaaattaata
     3421 ctacacgaac tagaatcggc gttgtggcgc agatataaaa attaatactg cacgaactag
     3481 aatcggcgtt gtggcgcaga tataaaaatt aatactgcac ggaaagaaac aactatagag
     3541 atcaaaacac attatattgt tttaaggagg cgtatattac gcctcttttt tattaaattt
     3601 atggagtgaa taacaatgga agttgttcgt ttaaatcaaa acctatttaa taaactacga
     3661 ggaaacgaaa tatcaagcaa taaaaatggt tcaagaccat attattattc tttcaaacgt
     3721 aataataata gagtttgtat accttttaga acaaatgcac aaaaggtacc taataaatat
     3781 aaaattaatt taggaggcga acagcccgat aaacctaatt cagctattga tttaacaaag
     3841 tctatagtaa ttagtaatga tgaatatctt aataacaggt caaaagctaa aataccacaa
     3901 aatgttaata attttttaaa gcaacaagca ccagctattg aacaaaaata cgatactatg
     3961 tctaatgatt acataaaagc aaaagctagt ttaagtaaga tacctttagt taaatattca
     4021 actatgcaat acttccacaa agaattgaat attcaagata gtattgataa tcaacaaact
     4081 aaaaatgcta ttaatgaatt aatatctaat gggaaatcaa ataaatataa taaattacaa
     4141 tcttctctgc ctaatgaaaa attaaatttg ctagatgatt atgaaactct atatgagttt
     4201 aaaagcctaa cagactaccc agctaaaata aattctaatg atattgataa tcctttctta
     4261 gaagttgaga aaaataacaa acattttact ttgtctgcac taactattaa aaatgaacca
     4321 gaaaagcatg taaaagactt tttaaattat gatatagaga atgaaaagaa taaagatatt
     4381 gatttagatt tataaggcct gtaaaaaagg ccttttttct ttttatttat tatgacacga
     4441 catgaaatga cacgtcacaa atggtataat tactatatgg aggttatatt tacattaggt
     4501 ttttattaaa aaaaggagtg atttaataat gagatttaca ccccgaggaa ttttgaacaa
     4561 caccgattat gaactaaatt ctgaatttta ttatgtgcca gcaagtgata aacaaatgaa
     4621 tgatcaaaga gatatgacta ataaaagcat agtaggtaat ttagtctata gaaataattt
     4681 ctataacgaa gtgtatactc aaccagttta tgatttagac gattctaacg aaaaatatca
     4741 aacaccacat attataagta tagatgattc tgaatttgat tataacttaa ctaatttaga
     4801 tgattatttt actaaagata cagcaaataa aggttatgaa aagctagtaa atgccattaa
     4861 atcagaagaa attacctatt ttgacagtga tattaatgaa gttgatgatt ttacacaaca
     4921 agatgaatat tcaaatgatt ggtatactaa cacttttgtg gatttagtat taagtatgaa
     4981 aggatattca tatcatgtag aaccaaatag agaatctgtt attgaggatt cacatatcat
     5041 aacagaaaat gatattactt ataatgtaag ctataacccc gaaaaacatc atttatatat
     5101 taatgacttc ccagcaattc caatagaaga aaacgataat ataagattat taaatgataa
     5161 cttaactcaa gacatggaaa aaacttacga taatataaac tataattttt catacaatga
     5221 ttttaaaaca cttgaagatg attcttatat ttatattgat ttaacagatt ataaagataa
     5281 cgaaggtaaa tttgttaaaa atctaataga agatatacca cctaatgaaa ttattaaaga
     5341 tagattaaaa caaaatatta atttatttga cgaacgagca aattataatg ccttagctgt
     5401 ttatagaagg gatttagaag atttttcaga tagaaataat ttagatagct tagataaagt
     5461 taaagattta aaaacattct atgatacgga aattaatatt aaaggattga acattaacaa
     5521 cgatattgag aaaaaggctt taaataatca attagccttt aacctttata atgaaccgtt
     5581 aaataacatg tttagagata acaatgtttt atatgaagtt tatgaaaaag aaatttatga
     5641 tgaaaaaatt cccctttcta tagaagatag aaaagaagtg aaaacttatt tagatgacgc
     5701 tagaaacaat ttttcaaata atttctatga atatacaacg aacaaatcat ctattttagc
     5761 tgaaaatagt ttaactgttt tagataaagc taaatatgat ttagaatttg cccctgtatc
     5821 taatcattta tacaaagaaa gagaaaattt ttacgaagga atgaaacaag ctaccctgcc
     5881 cttacacaat attactaaag aacaatataa agaaagagaa aatgatattg aaataatcgg
     5941 tcattttaaa aactatatca atactgaatt acatggaaca aagagttctg aattcaaacc
     6001 taatttcgta aatgattctt tagaaaatga aactaactca atagcttttg aagatattat
     6061 aaatggatca gaagaaacac atagtaatat tattaattct attaaaaatg aattaaagca
     6121 agaaagagat aatttagaaa tgtaggaggt acaatatgct aaaaacatat actgaaatca
     6181 ttggaagtga attgccaatt tatataacaa aatcagaaac aagcgaatta ctattttcag
     6241 aagaattaga taaaaattta aaatatgaag ttgctggata ttatctatat aatgctaaaa
     6301 aagacttata caaagaactt tataaaccta ttagcgaggt gtagtatatg gcttattcaa
     6361 aaggaaatta taaaaagaaa aaaatagatt caataataga agaattgaat aataagctag
     6421 atgacttcaa aaacaatgac aaaacctata aggaattttt agatactaca gcgaaatttc
     6481 acaactattc aattaataat attattctaa ttgctggcca aaatccagag gctacagctg
     6541 tagctggtta taaagcatgg aaaaataaat ttgatagaca agtgcaaaaa ggtgcaaaaa
     6601 gtatgaatat aattgcccct attgtacaaa aacgaaatgt agaaattaaa gatgataacg
     6661 gtaatatagt acgtgataat aacggtaatc ctaaaactga acgaaaaccc gtaattactg
     6721 gatatagagc gcataatgtg tttgatgtgg cagatacgaa aggcaagcct ttaataacag
     6781 caaaagacct aataaaaacc gaatttgaaa actctaacga ctataaagac ttatataatg
     6841 aatttaaaga ttatattaat gaagaattaa cacctagtgt agaggaaaaa cattttcttg
     6901 atgatcctac tttatctaat ggcgctaaag gttattatag tcctaaatct gatgaaattg
     6961 taatctctga tgatttgtca tatgatatgc gctttaaaac tttaatacat gaatatgcac
     7021 actctcaatt acacaataat gatataggta aaacgcaaat atctgaacat agtagatcat
     7081 taaaagaaat cgaggctgaa agttctgctt atgtagtagc taattattac ggtttagaca
     7141 ctagcgatta ttctttaggt tatttaagtg gctgggggca taatataagt gatgatgaat
     7201 taaaagccca cataaaaaat atacattcgt tcgcaaaaac tacaattgaa gaaataaatt
     7261 cattgccaga gttttctcaa tatatagata aaaaattaga atcagaaatg aacaaagatg
     7321 tatataaaga cctttcaaca atgatagata ccaatctaaa aaatggtttt gataaaataa
     7381 ctattatcaa aggtaattta gttaatgatt atggacttaa tgaaatttct gagaatagtt
     7441 atgaaaatga tgattttaaa gtgaatattg attacaaagg atttaatact aataattcgc
     7501 aagatcaagc taaaattgag ttaattaata agcatgatga ttcattgaat agagattaca
     7561 acttcacgca aacttataac cgaaatgtaa ttaataacac cacaactatt tttgtacaag
     7621 atgatgataa tgaagataaa aacaaaaaat atatccatga acgagatata gacggtaata
     7681 tattagaaga aaaacataaa ttaagcccac aagatgaaat agtatcattt gaaaactttg
     7741 taaataaatc agttgaagaa aatggcctaa aagatacatt acgccaattt gtaagtaatg
     7801 gtgtattaat gggctatgaa ttaaatgttg ataatattga taatacaaat gaaacttact
     7861 tttcaatgaa taaaaatcaa caaaatggcc aacgatcaaa tatgactgct aaattagaac
     7921 atgatactaa cgaaaatgtt tatatagatt ttaaaattaa aaattcagct ggtattaaaa
     7981 acatatccta tactgaaagt aatgaagaat ttaataaaga tgaacagaat aaagaaattg
     8041 aaaacaaaca agaagaagaa attgatatat aaaaagaagg catatacgcc ttcttttttt
     8101 atacaatact atttataagt gcatacctct tttatttttc aactcatggc gctgtttact
     8161 gtgattctta cctttgtact gatgatttat atacttcggc atacgttctc taaatatctc
     8221 attaaaatta aatatcattg aatctagtcc agcatttatt aaaccatagg cttgttgttt
     8281 tctattgtaa ttattgtaag tttctttatc tactagaata tcattacctt cataaaactc
     8341 ttcaatgtta tctttgctta tttcgtcccc tacttcattg gattcttgat ataaatgttt
     8401 taaatcattg ttatcactat aatcaaagtc atattcactc aataatttct tttgaatagc
     8461 cccaaataca aatttatcat gctgattttt attaggttta aattcttttt cttgtaacaa
     8521 cttaacttgt tcagttatat attttctatc ttcatcactg tatttaggaa taaaattacc
     8581 tgtttctaac atttcaattg tgttttctgt accatgataa gttttaaaat caattaattg
     8641 ttgtttttct ttaatgtcag ttacttcatt aaaagcttta taatcttcat catctgtaaa
     8701 taattccttt atttgtacat cataataatc ttctagtata ttttcagcat ttctaaaaac
     8761 ttctaatttg ttagtataat aatctatttt caaactatct tccatacctt catgtttatt
     8821 ctctaacaca tcatcaaaag acaagttatt tttttcgaga atatttgaaa gtttatcggc
     8881 aaagaaattc gattcttttt caatagattc taaacttata tctctgttac ctaaaactgt
     8941 attaaggctt atttgagcct cttttatgcg tttctcttta actatatcct ctagttcatt
     9001 attagctggt aaaacatctc tatcatttaa tatagtgcgt gtgataaaga ttttttcatc
     9061 atcactatag tttaatgatt gatcgggata atttttatca aagaaaattc cagcttgttt
     9121 ttcaaatacc tcatttatag attcaagttt tttctgtcta tcctctatat cctcaatttt
     9181 atttaatcta tcttcatcat cagtgattaa tgaagttttt tgttttaaat catatagtct
     9241 ttcattttcc ttatacatat ttataggtgt tacataaatt tttaaatctt tggccatatt
     9301 ttttaatgta gtagaatctg atttattcat ataactaaaa gcatttaatt tgtgagattt
     9361 catatcatct acttcattaa acatcttatc aattttttgc tgatttctct ttttaatact
     9421 ttcattatgt ttcgatattg aagatttttt tcctgtttca tcttcaaatt ttcgtgcttt
     9481 ccaaccttct ttttttgttg gctctaaatc tatattttga tttttatatg aatcagcaga
     9541 aactctaaca gaaaaaccat tcttttctag gtgtttattt tgaacatttt cccaccgttt
     9601 tcgccattca ttatgttttt cttttgtatt ccaatctcta atataatcat tgccctttcg
     9661 cttaggttca aattcattat ttttatctaa accacgtaat gtacataata aatgtgcgtg
     9721 aggattttct tcatttattt tatgaatatt tagatcaaca atcattcctt cattagataa
     9781 ggattcagca aattcttttg ctaattcaat attactattt ggatcaaatt cattcggcaa
     9841 accaattata atttctcgtg caacttgact attttttctg ttctcaacat catgaacttt
     9901 attccacaaa tattctctat ctttaaactt atcatcagca ttattaggta ataaaatttc
     9961 actataatca cattgcttat ttgagtaatc tttaaattcg ttttccttaa aatctttaat
    10021 cctactagcg ctattatagg cactttttgc agttgcactt tgtccatttg cttttgatac
    10081 aaacttattt tgaaaatggt acattgccat aataaaatct ccttaaaaaa caaatattga
    10141 gcgaaggcga aataaattaa aaattaattt ttaatttcac gaatttagca cgcgaacgga
    10201 acgttcgcat aagtgcgccc ttacgggatt taactagatt ataacggtaa aacttgaatc
    10261 tgtcaaacga aaaggttata attaaattaa taaaaaatat ggaggtttta tttatggctg
    10321 atattcaaaa aatgttagaa caaaaagaaa aacttcaaga aaaaattaaa gaagaaaaga
    10381 aaaaagaata tgaaaaattt ggaagatggt tcttcaataa atttaaagta aatagatcag
    10441 cagacgcaaa gaaaattata aataaaaatt tgcttaatat tgaagatact aaagaacaca
    10501 atttagaaag aaatgaaatt atagaagaaa ctaaaaattc caatgaagaa aacaaagaaa
    10561 taaatgaaat aaaccacaat atttaataga tattaactaa tattaatatg atgaaagact
    10621 tataaaataa ttggtttact aatagcaaaa tctacagtag tcaatacttt aaaaatagga
    10681 ggttttatta tgattttatt atcaacagct ggcgaaaatg ctgttcatat taaccctatt
    10741 tttagatttt ttctatattt tattatgtgt gttcctatga cagttgcttt atgtacaata
    10801 ttccttaaat ttatctcaat ttttattctt ccatggaaag tatgtttgta tattggtatt
    10861 gctttaggta ttattgttac tatttttctt ttggttataa caattggtat agaaatgaca
    10921 taaattttct acgaaaatat caaacatgac atgacatgta ataaagtatg tgttaaaatt
    10981 cattataaga agtagcaatc aagtaaataa atgctggttg ttattttttt acccacacag
    11041 tatatactag tatatactaa gatatactat aatatatact gatatatact aggatatatc
    11101 agtatagaaa agtatataaa taacttgtgg tattttatta attgtactaa tttaaaaaga
    11161 gtaaataata tgtggaggtt ttattatgag taacgtatat gtaatggctt tagattttgg
    11221 taatggattt gtaaaaggga agattaatga tgaaaaattt gtaatccctt caagaatcgg
    11281 aagaaaaact aatgaaaaca atcaattaaa aggatttgtt gataataaat tagatgtatc
    11341 agaatttatt atcaatggga acaatgatga agtattacta tttggtaatg atttagataa
    11401 aactacaaat actggtaaag atactgcctc aacaaatgat agatatgata ttaaatcttt
    11461 caaagatttg gttgaatgtt ctataggatt attagcaaga gaagtaccag aagaagttgt
    11521 caacgttgta attgctactg gtatgccaag taatgaaata ggaactgata aacaagctaa
    11581 gtttgaaaaa cttttaaata aaagtcgttt aattgaaatt gacggtatcg ctaaaacaat
    11641 taatgttaaa ggcgttaaga tagtagctca acctatggga actttacttg atcttaatat
    11701 ggaaaatgga aaagtattca aagcatttac agaaggcaag tacagtgttc ttgactttgg
    11761 tagtggtact acaattattg atacttatca aaatatgaaa cgtgtagagg aagaaagttt
    11821 tgttataaac aaaggtacta ttgattttta taaacgaatt gctagtcacg tatctaaaaa
    11881 atcagaaggt gcctcaatca caccacgtat gattgaaaaa ggattagaat acaagcaatg
    11941 taaacttaat caaaaaacag ttattgactt taaagatgaa ttttataagg aacaggatag
    12001 tttgatagaa gaagttatga gtaactttga aatcactgta ggtaatataa attcaattga
    12061 cagaattatt gttactggtg gtggtgcaaa cattcatttt gattcattaa gtcattatta
    12121 ttcagatgtg ttcgagaaag cagatgatag ccagtttagt aatgttcgtg gatatgaaaa
    12181 attgggggaa ttgttaaaaa acaaagtaga gcaagaaagt aaataattat ggataagaaa
    12241 gaaactaaac atcttctgaa aataaaaaaa gaagattacc ctcaaatctt cgatttttta
    12301 gaaaatgttc ctagaggtac taaaacagcc cacataagag aggcattgag gcgctatatt
    12361 gaagaaatag gggaaaaccc tccaatacct tcaaaagaac acacagcgcc cttaaaagag
    12421 catacagaac ctattaaacg taataaagaa agttttaaag acgcaatgga acgtaaggat
    12481 caattaaaac aagctgaaat gcctaaaata gaagaaaaag atgaagaaat ggtagatatt
    12541 agtattgatg acctttaata tctatattcc ttcatccaat tccatttttt cttctgcatt
    12601 tttaactacc ttatgaaagt ctacaccata gtattcagac attactaaaa atgtatgtaa
    12661 tgctattcta ggttttttac cattctcgat acgtgaaaga tgaccagcag tcacactcaa
    12721 atcaaatgca acttcttctt gtcgcttgcc tatgagttga cgctggtttt taaattctaa
    12781 acaaatgtat ctatatagtt tttgtatacg gtattcattc atgatttatc ggcctcataa
    12841 ttttagatat attaattata ttagagattt gttatcaaat acatttcatt acaagcatat
    12901 aattgacctg tgaggcaaca aaaaattttt aattttttta aaatagtgtt actattgaac
    12961 atagaaaaag acacctaacg cttggcggtt gttagatgtc taaagagttt aaataatatg
    13021 tgttatttac ttttacaatt tgtttgtatc tttattatat atacaacctc gatacattac
    13081 aagataaaag caacaagtct ttaagttata tataacggat aacattatat aaccttattc
    13141 atcacttgta aataacgata ttacttaaac tccttaaata tacatttaaa ggagttttta
    13201 tcatgtctaa acaatttttt acagtagaag aaaattataa agaacgcttt taccaattac
    13261 ctaaagtatt ttttactaat cctaattata aagatttatc gaatgacgct aaaattgctt
    13321 acgcaatatt gagagatcgt ttacaattat ctattaaaaa taactggata gatacagaag
    13381 gtaacatata tttcatttat acagttgctg atttagaagt gatattaaat tgtgggaaca
    13441 aaaaaatcac taaaattaaa aaagaattag aaaatgtaga tttactaatc caaaaaaggc
    13501 agggtttaaa caaaccaaat ctactttatt tattaaaacc agccataact aaaaatgata
    13561 tttatgaaat agataaggct gaaaatgaag ttgaggcctt acaagacaag gaagtgtcaa
    13621 aaggacacgt ccagaaatgt caaaaggaca cgtctagaaa tgtcaaaagg acacgtctag
    13681 aaatgtcaaa aggacacaca aatgatactg attttattga tactgatttt attgatactg
    13741 aaagtaatga tatgaataat atgaatgata caaatcaaca ttcaaatcat tcaaatcact
    13801 tttcaaatat tcatgataaa gaatctctta aatatataga acttcaagaa ctccccgaat
    13861 taattaaaag ttatattaat aatttcagct atgaagaagt taaaagtatt aaatctgtta
    13921 ttttaaaagc taaaaaatca tttaataaca aatacgatac tttctatatg ttagaagata
    13981 tagatgaaga attactattg gttttaaaac gatttaaagg ttatcttgtt aaaaaacaag
    14041 aaaaagtagc taatatggaa ggttatttaa tgagaagtat cattgctgaa cttgaagaaa
    14101 tgcactcaac tattatgaga agaaaaaata tggaaaacaa tccattatct ctatttaatt
    14161 agtttttgaa gttaaatttt caaaaaaaag agattataca cctaaaaagg tgtataatct
    14221 tta
//
LOCUS       DAXWFK010000057        14185 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.057, whole genome shotgun sequence.
ACCESSION   DAXWFK010000057 DAXWFK010000000
VERSION     DAXWFK010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14185
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.057"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            137..430
                     /gene="esxA"
                     /locus_tag="ACGEXS_001319"
     CDS             137..430
                     /gene="esxA"
                     /locus_tag="ACGEXS_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion effector EsxA"
                     /protein_id="HFF8012337.1"
                     /translation="MAMIKMSPEEIRAKSQSYGQGSDQIRQILSDLTRAQGEIAANWE
                     GQAFSRFEEQFQQLSPKVEKFAQLLEEIKQQLNSTADAVQEQDQQLSNNFGLQ"
     gene            514..3543
                     /gene="esaA"
                     /locus_tag="ACGEXS_001320"
     CDS             514..3543
                     /gene="esaA"
                     /locus_tag="ACGEXS_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaA"
                     /protein_id="HFF8012338.1"
                     /translation="MKKKNWIYALIVTLIIIIAIVSMIFFVQTKYGDQSEKGSQSVSN
                     KNNKIHIAIVNEDQPTTYNGKKVELGQAFIKRLANEKNYKFETVTRNVAESGLKNGGY
                     QVMIVIPENFSKLAMQLDAKTPSKISLQYKTAVGQKEEVAKNTEKVVSNVLNDFNKNL
                     VEIYLTSIIDNLHNAQKNVGAIMTREHGVNSKFSNYLLNPINDFPELFTDTLVNSISA
                     NKDITKWFQTYNKSLLSANSDTFRVNTDYNVSTLIEKQNSLFDEHNTAMDKMLQDYKS
                     QKDSVELDNYINALKQMDSQIDQQSSMQDTGKEEYKQTVKENLDKLREIIQSQESPFS
                     KGMIEDYRKQLTESLQDELANNKDLQDALNSIKMNNAQFAENLEKQLHDDIVKEPDTD
                     TTFIYNMSKQDFIAAGLNEDEANKYEAIVKEAKRYKNEYNLKKPLAEHINLTDYDNQV
                     AQDTSSLINDGVKVQRTETIKSNDINQLTVATDPHFNFEGDIKINGKKYDIKDQSVQL
                     DTSNKEYKVEVNGVAKLKKDAEKDFLKDKTMHLQLLFGQANRQDEPNDKKATSVVDVT
                     LNHNLDGRLSKDALSQQLSALSRFDAHYKMYTDTKGREDKPFDNKRLIDMMVDQVIND
                     MESFKDDKVAVLHQIDSMEENSDKLIDDILNNKKNTTKNKEDISKLIDQLENVKKTFA
                     EEPQEPKIDKGKNDEFNTMSSNLDKEISRISEKSTQLLSDTQESKSIADSVSGQLNQV
                     DNNVNKLHATGRALGVRANDLNRQMAKNDKDNELFAKEFKKVLQNSKDGDRQNQALKA
                     FMSNPVQKKNLENVLANNGNTDVISPTLFVLLMYLLSMITAYIFYSYERAKGQMNFIK
                     DDYSSKNHLWNNVITSGVIGTTGLVEGLIVGLIAMNKFHVLAGYRAKFILMVILTMMV
                     FVLINTYLLRQVKSIGMFLMIAALGLYFVAMNNLKAAGQGVTNKISPLSYIDNMFFNY
                     LNAEHPIGLVLVILTVLVIIGFVLNMFIKHFKKERLI"
     gene            3543..4001
                     /gene="essA"
                     /locus_tag="ACGEXS_001321"
     CDS             3543..4001
                     /gene="essA"
                     /locus_tag="ACGEXS_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssA"
                     /protein_id="HFF8012339.1"
                     /translation="MLMNSVIALTFLTASSNNGGLNIDVQQEEEKRINNDLNQYDTTL
                     FNKDSKAVNDAIAKQKKERQQQIKNDMFQNQASHSTRLNETKKVLFSKSNLEKTSESD
                     KSPYIQNKQEKKIFPYILMSVGAFLTLGFVIFSIHKGRRTKNESARKSNI"
     gene            3973..4215
                     /gene="esaB"
                     /locus_tag="ACGEXS_001322"
     CDS             3973..4215
                     /gene="esaB"
                     /locus_tag="ACGEXS_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaB"
                     /protein_id="HFF8012340.1"
                     /translation="MNQHVKVTFDFTNYNYGTYDLAVPAYLPIKNLIALVLDSLDISI
                     FDVNTQIKVMTKGQLLVENDRLIDYQIADGDILKLL"
     gene            4228..5562
                     /gene="essB"
                     /locus_tag="ACGEXS_001323"
     CDS             4228..5562
                     /gene="essB"
                     /locus_tag="ACGEXS_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssB"
                     /protein_id="HFF8012341.1"
                     /translation="MVKNHNPKNEMQDMLTPLDAEEAAKTKLRLDMREIPKSSIKPEH
                     FHLMYLLEQHSPYFIDAELTELRDSFQIHYDINDNHTPFDNIKSFTKNEKLRYLLNIK
                     NLEEVNRTRYTFVLAPDELFFTRDGLPIAKTRGLQNVVDPLPVSEAEFLTRYKALVIC
                     AFNEKQSFDALVEGNLELHKGTPFETKVIEAATLDLLTAFLDEQYQKQEQDYSQNYAY
                     VRKVGHTVFKWVAIGMTTLSVLLIAFLAFLYFSVMKHNERIEKGYQAFVKDDYTQVLN
                     TYDDLDGKKLDKEALYIYAKSYIQTNKQGLEKDKKENLLNNVTPNSNKDYLLYWMELG
                     QGHLDEAINIATYLDDNDITKLALINKLNEIKNNGDLSNDKRSEETKKYNDKLQDILD
                     KEKQVKDEKAKSEEEKAKAKDEKLKQQEENEKKQKEQAQKDKEKRQEAERKK"
     gene            5584..10023
                     /gene="essC"
                     /locus_tag="ACGEXS_001324"
     CDS             5584..10023
                     /gene="essC"
                     /locus_tag="ACGEXS_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssC"
                     /protein_id="HFF8012342.1"
                     /translation="MHKLIIKYNKQLKMLNLRDGKTYTISEDERADITLKSLGEVIHL
                     EQNNQGTWQANHTSINKVLVRKGDLDDITLQLYTEADYASFAYPSIQDTMTIGPNAYD
                     DMVIQSLMNAIIIKDFQSIQESQYVRIVHDKNTDVYINYELQEQLTNKAYIGDHIYVE
                     GIWLEVQADGLNVLSQNTVASSLIRLTQEMPHAQADDYNTYHRSPRIIHREPTDDIKI
                     ERPPQPIQKNNTVIWRSIIPPLVMIALTVVIFLVRPIGIYILMMIGMSTVTIVFGITT
                     YFSEKKKYNKDVEKREKDYKAYLDNKSKEINKAIKAQRFSLNYHYPTVAEIKDIVETK
                     APRIYEKTSHHHDFLHYKLGIANVEKSFKLDYQEEEFNQRRDELFDDAKELYEFYTDV
                     EQAPLINDLNHGPIAYIGARHLILEELEKMLIQLSTFHSYHDLEFLFVTREDEVETLK
                     WARWLPHMTLRGQNIRGFVYNQRTRDQILTSIYSMIKERIQAVRERSRSNEQIIFTPQ
                     LVFVITDMSLIIDHVILEYVNQDLSEYGISLIFVEDVIESLPEHVDTIIDIKSRTEGE
                     LITKEKELVQLKFTPENIDNVDKEYIARRLANLIHVEHLKNAIPDSITFLEMYNVKEV
                     DQLDVVNRWRQNETYKTMAVPLGVRGKDDILSLNLHEKAHGPHGLVAGTTGSGKSEII
                     QSYILSLAINFHPHEVAFLLIDYKGGGMANLFKDLVHLVGTITNLDGDEAMRALTSIK
                     AELRKRQRLFGEHDVNHINQYHKLFKEGIATEPMPHLFIISDEFAELKSEQPDFMKEL
                     VSTARIGRSLGIHLILATQKPSGVVDDQIWSNSKFKLALKVQDRQDSNEILKTPDAAD
                     ITLPGRAYLQVGNNEIYELFQSAWSGATYDIEGDKLEVEDKTIYMINDYGQLQAINKD
                     LSGLEDEETKENQTELEAVIDHIESITTRLEIEEVKRPWLPPLPENVYQEDLVETDFR
                     KLWSDDAKEVELTLGLKDVPEEQYQGPMVLQLKKAGHIALIGSPGYGRTTFLHNIIFD
                     VARHHRPDQAHMYLFDFGTNGLMPVTDIPHVADYFTVDQEDKIAKAIRIFNDEIDRRK
                     KILSQYRVTSISEYRKLTGETIPHVFILIDNFDAVKDSPFQEVFENMMIKMTREGLAL
                     DMQVTLTASRANAMKTPMYINMKTRIAMFLYDKSEVSNVVGQQKFAVKDVVGRALLSS
                     DDNVSFHIGQPFKHDETKSYNDQINDEVSAMTEFYKGETPNDIPMMPDEIKYEDYRES
                     LNLPDIVANGALPIGLDYEGVTLQKIKLTEPAMISSENPREIAHIAEIMMKEIDILNE
                     KYAICIADSSGEFKAYRHQVANFAEEREDIKAIHQLMIEDLKQREMDGPFEKDSLYII
                     NDFKTFIDCTYIPEDDVKKLITKGPELGLNILFVGIHKELIDAYDKQIDVARKMINQF
                     SIGIRISDQQFFKFRFIQREPVIKENEAYMVANQAYQKIRWFK"
     gene            10053..10445
                     /gene="esaC"
                     /locus_tag="ACGEXS_001325"
     CDS             10053..10445
                     /gene="esaC"
                     /locus_tag="ACGEXS_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion substrate EsaC"
                     /protein_id="HFF8012343.1"
                     /translation="MNFNDIETMVKSKFKDIKKHAEEIAHEIEVRSGYLRKAEQYKRL
                     EFNLSFALDDIESTAKDVQTAKSSANKDSVTVKGKAPNTLYIEKRNLMKQKLEMLGED
                     IDKNKESLQKAKEIAGEKASEYFNKAMN"
     gene            10461..10775
                     /gene="esxB"
                     /locus_tag="ACGEXS_001326"
     CDS             10461..10775
                     /gene="esxB"
                     /locus_tag="ACGEXS_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion effector EsxB"
                     /protein_id="HFF8012344.1"
                     /translation="MGGYKGIKADGGKVDQAKQLAAKTAKDIEACQKQTQQLAEYIEG
                     SDWEGQFANKVKDVLLIMAKFQEELVQPMADHQKAIDNLSQNLAKYDTLSIKQGLDRV
                     NP"
     gene            10775..11449
                     /locus_tag="ACGEXS_001327"
     CDS             10775..11449
                     /locus_tag="ACGEXS_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5081 family protein"
                     /protein_id="HFF8012345.1"
                     /translation="MKDVKRIDYFSYEELTILGGSKLPLVNFELFDPSNFEEAKAALI
                     EKELVTENDKLTDAGFKVATLVREYISAIVNIRINDMYFAPFSYEKDEYILLSRFKNN
                     GFQIRIINKDIAWWSIVQSYPLLMRQEKSNDWDFKQIDDETLENLNNESIDTIGRVLE
                     IEIYNHQGDPQQSLYNIYEQNDLLFIRYPLKDKVLNVHIGVINTFIRELFGFDTDENH
                     INKAEE"
     gene            11449..11766
                     /gene="esxD"
                     /locus_tag="ACGEXS_001328"
     CDS             11449..11766
                     /gene="esxD"
                     /locus_tag="ACGEXS_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion system extracellular protein
                     EsxD"
                     /protein_id="HFF8012346.1"
                     /translation="MTLSGKISVKAETIAHVVKELESISQKYDEIAQNFGKIAQLNYY
                     SSEKAAHSMENGYSSAATVISGLKGPLSTLGGGVMNSAQKFFEADEHWGTEFAKLYYN
                     IEG"
     gene            11776..13620
                     /locus_tag="ACGEXS_001329"
     CDS             11776..13620
                     /locus_tag="ACGEXS_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFF8012347.1"
                     /translation="MTKDIEYLTADYDNEKSSIQSVIDAIEGQDFLDVDTTMDDAVSD
                     VSSLDEDGAISLTSSVVGPQGSKLMGYYQNELYDYASQLDSKMKEIIDTPFIEDIDKA
                     FKGITNVKLENILIKNGGGHGRDTYGASGKIAKGDAKKSDSDVYSIDEILKSDQEFVK
                     VIDQHYKEMKKEDKKLSKSDFEKMMTQGASCDYMTVAEAEELEEQKKKEEAIEIAALA
                     GMVVLSCINPVAGAVAIGAYSAYSAANAATGKNIVTGRKLSKEERIMEGLSLIPLPGM
                     GFLKGAGKSLMKLGFKGGEKFAVKTGLQKTMQQAVSRISPKMGMMKNSVLNQSRNFAQ
                     NTHVGQMLSNMRGQATHTVQQSRNWIGQQAQNVKRIVNNGLDKEIAHPFKQQLAPAGM
                     GGIKFAETTTLRNMGQNIKRAVTPQNHVTHGPKDSMVRSEGKHSISSHEMNSSKYVES
                     PNYTKVEFGEHYARLRPKKLKANIEYTTPTGHIYRTDHKGRIKEVYVDNLSLKDGDRN
                     SHAQRTVGGEDRLPDDDGGHLIARMFGGSKDIDNLVAQSKFINRPFKEKGHWYNLEKE
                     WQEFLNSGKEVKNIKMEVKYSGNSQRPTIFKVEYEINGERNIRRILNK"
     gene            13631..14122
                     /locus_tag="ACGEXS_001330"
     CDS             13631..14122
                     /locus_tag="ACGEXS_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HFF8012348.1"
                     /translation="MTFEEKLSKIYNEIANEISSMIPVEWEKVYTMAYIDDGGGEVFF
                     NYTKPGSDDLNYYTNIPKEYNISVQVFDDLWMDLYDLFEELRDLFKEEDLEPWTSCEF
                     DFTREGELKVSFDYIDWINSEFGQIGRQNYYKYRKFGILPETEYEINKVKEIEQYIKE
                     LEE"
BASE COUNT         5539 a         1951 c         2689 g         4006 t
ORIGIN      
        1 aaaaagctaa aaatacttaa aaaactgtat acatatacta ataatttata tattatttga
       61 gtaaggagca ctttttcaaa aaatagtgtc cctaaaaagt tttgataaac ttaaaatatt
      121 caggaggttt ctagttatgg caatgattaa gatgagtcca gaggaaatca gagcaaaatc
      181 gcaatcttac gggcaaggtt cagaccaaat ccgtcaaatt ttatctgatt taacacgtgc
      241 acaaggtgaa attgcagcga actgggaagg tcaagctttc agccgtttcg aagagcaatt
      301 ccaacaactt agtcctaaag tagaaaaatt tgcacaatta ttagaagaaa ttaaacaaca
      361 attgaatagc actgctgatg ccgttcaaga acaagaccaa caactttcta ataatttcgg
      421 tttgcaataa gcattctgaa attggcaaag tcacattttc taatgtggct ttgcttatca
      481 tttttttaag aaaacaactg aaaggaaata agcatgaaaa agaaaaattg gatttatgca
      541 ttaattgtca ctttaattat tataattgcc atagttagta tgatattttt tgttcaaaca
      601 aaatatggag atcaatcaga aaaaggatcc caaagtgtaa gtaataaaaa taataaaata
      661 catatcgcaa ttgttaacga ggatcaacca acgacatata acggtaaaaa agttgagctg
      721 ggtcaagcat ttattaaaag gttagcaaat gagaaaaact ataaatttga aacagtaaca
      781 agaaacgttg ctgagtctgg tttgaaaaat ggtggatacc aagtcatgat tgttatccca
      841 gaaaactttt caaaattggc aatgcaatta gacgctaaaa caccatcgaa aatatcgcta
      901 cagtataaaa cagctgtagg acaaaaagaa gaagtagcta aaaacacaga aaaagttgta
      961 agtaatgtac ttaacgactt taacaaaaac ttagtcgaaa tttatttaac aagcatcatt
     1021 gataatttac ataatgcaca aaaaaatgtt ggcgctatta tgacgcgtga acatggtgtg
     1081 aatagtaaat tctcgaatta cttattaaat ccaattaacg acttcccgga attatttaca
     1141 gatacgcttg taaattcaat ttctgcaaac aaagacatta caaaatggtt ccaaacatac
     1201 aataaatcat tattgagtgc gaattcagat acgttcagag tgaacacaga ttataatgtt
     1261 tcgactttaa ttgaaaaaca aaattcatta tttgacgagc acaatacagc gatggataaa
     1321 atgttacaag attataaatc gcaaaaagat agcgtggaac ttgataacta tatcaatgca
     1381 ttaaaacaga tggacagcca aattgatcaa caatcaagta tgcaagatac aggtaaagaa
     1441 gaatataaac aaactgttaa agaaaactta gataaattaa gagaaatcat tcaatcacaa
     1501 gagtcaccat tttcaaaagg tatgattgaa gactatcgta agcaattaac agaatcactg
     1561 caagatgagc ttgcaaataa caaagactta caagatgcgc taaatagcat taaaatgaac
     1621 aatgctcaat tcgctgaaaa cttagagaaa caacttcatg atgatattgt caaagaacct
     1681 gatacagata caacatttat ctataacatg tctaaacaag actttatagc tgcaggttta
     1741 aatgaggatg aagctaataa atacgaagca attgtcaaag aagcaaaacg ttataaaaat
     1801 gaatataatt tgaaaaaacc gttagcagaa cacattaatt taacagatta cgataaccaa
     1861 gttgcgcaag acacaagtag tttgattaat gatggtgtca aagtgcaacg tactgaaacg
     1921 attaaaagta atgatattaa tcaattaact gttgcaacag atcctcattt taattttgaa
     1981 ggcgacatta aaattaatgg taaaaaatat gacattaagg atcaaagtgt tcaactcgat
     2041 acatctaaca aggaatataa agttgaagtc aatggcgttg ctaaattgaa aaaggatgct
     2101 gagaaagatt tcttaaaaga taaaacaatg catttacaat tgttatttgg acaagcaaat
     2161 cgtcaagatg aaccaaatga taagaaagca acgagtgttg tggatgtaac attgaatcat
     2221 aaccttgatg gtcgcttatc gaaagatgca ttaagccagc aattgagtgc attatctagg
     2281 tttgatgcgc attataaaat gtacacagat acaaaaggca gagaagataa accattcgac
     2341 aacaaacgtt taattgatat gatggttgac caagttatca atgacatgga aagtttcaaa
     2401 gacgataaag tagctgtgtt acatcaaatt gattcaatgg aagaaaactc agacaaactg
     2461 attgatgaca ttttaaataa caaaaagaat acaacaaaaa ataaagaaga tatttccaag
     2521 ctgattgatc agttagaaaa cgttaaaaag acttttgctg aagagccaca agaaccaaaa
     2581 attgataaag gcaaaaatga tgaatttaat acgatgtctt caaatttaga taaagaaatt
     2641 agtagaattt ctgaaaagag tacgcaattg ctatcagata cacaagaatc aaaatcaatt
     2701 gcagattctg ttagtggcca attaaatcaa gtcgacaata atgtgaataa gctacatgcg
     2761 acaggtcgag cattaggcgt aagagctaac gatttgaatc gtcaaatggc taaaaacgat
     2821 aaagataatg agttgttcgc taaagaattt aaaaaagtat tacaaaattc taaagatggc
     2881 gacagacaaa accaagcatt aaaagcattt atgagtaatc cggttcaaaa gaaaaactta
     2941 gaaaatgttt tagctaataa tggtaataca gacgtgattt caccgacatt attcgtatta
     3001 ttgatgtatt tactatcaat gattacagca tatattttct atagttatga acgtgccaaa
     3061 ggacaaatga atttcattaa agatgattat agtagtaaaa accatctttg gaataatgtc
     3121 attacgtcag gtgttattgg tacaactggt ttggtagaag ggttaattgt cggtttaatt
     3181 gcaatgaata agttccatgt attagctggc tatagagcga aattcatctt aatggtgatt
     3241 ttaactatga tggtcttcgt acttattaat acgtatttac taagacaggt aaaatctatc
     3301 ggtatgttct taatgattgc tgcattgggt ctatactttg tagctatgaa taatttgaaa
     3361 gcagctggac aaggtgtgac taataaaatt tcaccattgt cttatatcga taacatgttc
     3421 ttcaattatt taaatgcaga gcatcctata ggcttggtgc tagtaatatt aacagtactt
     3481 gtgattattg gctttgtact gaacatgttt ataaaacact ttaagaaaga gagattaatc
     3541 taatgttgat gaatagcgtg attgctttaa cttttttaac agcatctagc aataatggcg
     3601 gacttaatat tgatgtgcaa caagaagagg aaaagcgaat caataatgat ttaaatcaat
     3661 atgatacaac gctatttaat aaagacagca aagcggttaa tgatgcgatt gctaagcaga
     3721 aaaaagaacg acaacaacaa ataaaaaatg atatgtttca aaatcaagcg agtcactcga
     3781 ctcgcttgaa tgaaactaaa aaagtgttat tttccaaatc taacttagaa aagacttcgg
     3841 agagtgataa aagcccctat attcaaaaca agcaggagaa aaaaatattc ccgtacattt
     3901 tgatgtctgt aggggctttt ttgactttag gatttgtcat tttttcaatt cataaaggga
     3961 gacgaacgaa aaatgaatca gcacgtaaaa gtaacatttg attttactaa ttataattac
     4021 ggcacatatg acttagcagt accagcatat ttaccgataa aaaacttaat agctttagta
     4081 ttggatagtt tggacatttc aatatttgat gtcaatacac aaattaaagt gatgacgaaa
     4141 ggtcaattac ttgttgaaaa tgatcgactc attgattatc aaatcgctga tggagatatt
     4201 ttgaagttac tataggagga aaaatagatg gttaaaaatc ataaccctaa aaatgaaatg
     4261 caagatatgt taacgccttt agatgctgaa gaagcagcta aaacaaaatt acgcttagat
     4321 atgagagaga ttcctaagtc ttcaattaaa ccagaacatt ttcatttaat gtacttatta
     4381 gaacaacatt ctccatattt tatagatgct gaattaactg aactacgtga cagtttccaa
     4441 atacattatg acattaatga caatcataca ccttttgata atattaaatc atttactaaa
     4501 aatgaaaaat tacgttactt actcaatatc aaaaatttag aagaagtaaa tcgtacacgc
     4561 tacacatttg tgttggcacc agatgaatta tttttcacaa gagatggatt acccattgct
     4621 aaaacaagag ggttacaaaa tgttgttgat ccattacctg tgtcagaagc tgaattttta
     4681 acaagatata aagcgctggt tatctgtgca ttcaatgaga aacaatcatt tgatgcttta
     4741 gttgaaggaa acttagaact acataaagga acgccatttg aaactaaagt tattgaagcg
     4801 gcaacgttag atttactaac ggcattttta gatgaacagt atcagaaaca agaacaagat
     4861 tatagtcaaa attatgcata tgtacgcaaa gtaggacata ccgttttcaa atgggttgct
     4921 atcggtatga caacgttaag tgttttatta attgcattct tagccttttt atatttttca
     4981 gtaatgaagc ataatgagcg cattgaaaaa ggataccaag catttgtaaa ggatgattat
     5041 acgcaagtac taaatacgta tgatgattta gatggtaaaa aattagataa agaggcactt
     5101 tacatttatg ccaaaagtta tatccaaaca aataaacaag gtttagaaaa agataagaaa
     5161 gaaaatttac ttaataatgt gacaccaaat tcaaacaaag actacttatt atattggatg
     5221 gaattaggac aaggacatct tgatgaagcg attaatattg ccacttattt agatgataac
     5281 gatattacaa agttagcgtt gattaataaa ttaaatgaga ttaaaaataa cggagattta
     5341 tcgaatgata aacgttctga agaaacgaaa aagtataacg ataaattgca agatatttta
     5401 gacaaagaaa aacaagttaa agatgaaaaa gcgaaatctg aagaagagaa agcaaaagcg
     5461 aaagatgaga aattaaagca acaagaagag aacgaaaaga aacaaaaaga acaagcacaa
     5521 aaagataaag aaaaacgcca agaagctgaa agaaaaaaat agtataggac tgaggcaaag
     5581 acaatgcata aattgattat aaaatataac aaacaattga agatgctcaa tttgcgagat
     5641 ggtaagacat atactattag cgaagacgag cgtgcagata ttacgttgaa atcgttaggc
     5701 gaagtcattc atttagaaca aaataatcaa ggtacttggc aagcgaatca tacttctatt
     5761 aataaggtgc ttgttagaaa aggtgacctt gatgacatta cattacagct ttatacagaa
     5821 gctgattatg catcatttgc gtatccttca attcaagata cgatgacaat tggaccaaat
     5881 gcgtatgatg atatggttat tcaaagcttg atgaatgcca tcattattaa agattttcaa
     5941 tcaatacaag aatcacaata cgtacgcatt gtgcacgata aaaatacaga tgtgtatatt
     6001 aactatgaac tacaagagca actaacgaac aaagcttaca ttggtgatca tatttatgtt
     6061 gaagggatat ggctcgaagt acaagctgat ggtttaaatg tattgagtca gaatacagtg
     6121 gcatcgtcat taattcgctt aacacaagag atgccacatg cacaggcaga tgattacaat
     6181 acgtaccatc gttcgccaag gattattcac cgtgaaccga cggatgatat taagattgaa
     6241 agaccgccac agccaataca gaagaacaat acagtgatat ggcgttccat tataccgcca
     6301 ttagtaatga ttgctttaac tgttgtcatc tttttagtga gaccaattgg tatttatatt
     6361 ttaatgatga ttggtatgag tacagtaacg atagtatttg gtattacaac gtatttctct
     6421 gaaaagaaaa agtataacaa agatgttgaa aaacgagaga aagattacaa agcttatttg
     6481 gataataaat ctaaagaaat taataaagcg attaaagcac aacgttttag tttgaattac
     6541 cattatccaa cggttgctga aattaaagat atcgttgaaa cgaaagcacc aagaatatat
     6601 gaaaaaacat cgcatcatca cgatttctta cattataagt taggtattgc gaatgtagaa
     6661 aagtcattca aattagatta ccaagaagaa gaatttaacc aacgtcgtga tgaactattc
     6721 gacgatgcta aagaattgta tgaattttac acagatgtag aacaagcacc attaatcaat
     6781 gatttaaatc atgggccaat tgcatatatt ggtgcacgac atctcatttt agaagaattg
     6841 gagaaaatgc taattcaatt gtcaacattc catagttatc atgatttaga gtttctattt
     6901 gtgacacgtg aagatgaagt tgaaacattg aaatgggcac gttggttgcc acatatgaca
     6961 ttgagagggc aaaacattag aggatttgtt tacaatcaac gaacgcgtga ccaaatttta
     7021 acgtcaattt atagcatgat taaagaacgt atccaagctg tgcgtgaacg cagcagaagt
     7081 aatgagcaaa ttattttcac accgcaatta gtgtttgtca ttacagatat gtcattaatt
     7141 attgatcatg tcattttaga atatgtaaac caagatttat cagaatatgg tatttcatta
     7201 atctttgttg aagatgtgat tgaaagtttg ccagagcatg tagataccat tattgatatc
     7261 aagtctcgta ctgaaggcga actgattacg aaagaaaaag aattagttca attgaaattt
     7321 acacctgaaa atattgataa cgtcgataaa gaatatatcg cgcgacgttt ggcgaatttg
     7381 atacacgtcg aacatttgaa aaatgcaatt cctgatagta ttacattttt agagatgtat
     7441 aacgtgaaag aagtagatca gcttgatgtg gttaatcgat ggagacaaaa cgaaacatac
     7501 aaaacgatgg cagtaccttt aggtgtaaga ggtaaagatg atattttatc attgaactta
     7561 catgaaaaag cacacgggcc acatggttta gttgctggta ccactggttc agggaaatct
     7621 gagattatcc aatcatacat tttatcttta gctattaatt ttcaccctca tgaagttgca
     7681 ttcctattga ttgactataa aggtgggggt atggcgaact tatttaaaga tttagtccat
     7741 ttagttggta cgattacaaa cttagatggc gatgaagcga tgcgtgcctt aacatcaatc
     7801 aaagccgaat tgagaaaacg tcaacgttta ttcggagagc atgatgttaa ccatattaat
     7861 caataccata agttatttaa agaaggtatt gcgacagaac caatgccaca tttattcatt
     7921 atttccgatg agtttgccga attaaaatca gaacaacctg attttatgaa agaacttgta
     7981 tcaacggcac gtattggacg ttcgttaggt attcatttaa tacttgcgac acaaaaacca
     8041 tcgggtgttg ttgatgacca aatttggtct aactctaaat ttaagttggc attaaaagta
     8101 caagatagac aagacagtaa tgaaatttta aaaacaccag atgcagcaga cattacatta
     8161 ccaggtcgtg cgtatttaca agttggtaat aatgaaattt atgaattatt ccaatctgca
     8221 tggagtggtg caacatatga catcgaaggc gataaattag aagttgaaga taagacgatt
     8281 tacatgatta atgactatgg tcaacttcaa gcaatcaaca aagacttgag tggacttgaa
     8341 gatgaagaaa cgaaagaaaa tcaaactgag ttagaagcgg tcatagatca tatcgaatct
     8401 attacaacac gattagaaat cgaagaagtt aagcgtccat ggctaccacc attgccagaa
     8461 aatgtatatc aagaagattt agtagaaaca gatttcagaa aattatggtc agatgatgca
     8521 aaagaagtgg aattaacatt aggacttaaa gacgtaccag aagaacaata tcaaggaccg
     8581 atggtattgc aattgaaaaa agctgggcac atcgcgttaa tcggaagtcc aggatatggt
     8641 agaacaacgt tcttacacaa cattattttc gatgttgcaa gacaccatcg tcctgatcaa
     8701 gcacacatgt acttgttcga tttcggtacc aatggtttga tgccagttac agacatacca
     8761 catgtcgctg attactttac agtagatcaa gaagacaaga ttgctaaggc gatacgtata
     8821 tttaatgatg aaattgatcg tcgtaagaag attttaagtc agtatcgtgt cactagtatt
     8881 tctgaatatc gaaaattaac tggtgaaaca attccgcatg tctttattct tattgataac
     8941 tttgacgcag taaaagattc acctttccaa gaagtttttg aaaatatgat gattaaaatg
     9001 acgcgtgaag ggctagcatt agacatgcaa gtaaccttaa ctgcttcaag agctaacgct
     9061 atgaaaacac caatgtacat taatatgaaa acgcgtatcg caatgttttt atatgataaa
     9121 tcagaggtgt cgaacgtagt aggacagcaa aaatttgcgg ttaaagatgt tgtgggtcga
     9181 gcattgttaa gtagtgatga caacgtatca ttccatattg gccaaccatt taaacatgat
     9241 gagaccaaat catataatga tcaaattaat gatgaagtat cggcgatgac agaattttat
     9301 aaaggtgaaa caccaaatga tattcctatg atgccagatg aaattaaata tgaagattac
     9361 agagaatcat taaacttacc agatatagtt gcaaatggtg ctttaccaat tggattagat
     9421 tatgaaggtg ttacactaca aaaaattaaa ttaactgaac cagcaatgat ttcatcagaa
     9481 aatccgagag aaattgcgca tattgctgaa attatgatga aagaaattga catattaaat
     9541 gaaaaatatg cgatttgtat cgcagactca agtggagagt ttaaagctta taggcatcaa
     9601 gtggctaact ttgccgaaga aagagaagac attaaagcga ttcatcaact aatgattgaa
     9661 gacttaaagc aaagagaaat ggacggccca tttgaaaaag attcacttta tattatcaat
     9721 gattttaaaa catttattga ttgcacgtat attccggaag atgatgttaa aaagcttatt
     9781 acaaaaggac cagaacttgg cttgaacatt ttatttgtcg gcattcataa agaattaata
     9841 gatgcttatg ataaacagat tgatgttgca cgtaaaatga ttaaccaatt tagtataggt
     9901 attcgtattt cagaccaaca attctttaaa tttagattta ttcaacgaga acctgttatt
     9961 aaagaaaatg aagcatatat ggtcgcaaac caagcttatc aaaagattag atggtttaaa
    10021 tagcaatgaa ttaaatagga gggaggtatg ttatgaattt taatgatatt gaaacaatgg
    10081 ttaagtcgaa atttaaagat attaaaaagc atgctgaaga gattgcgcat gaaattgaag
    10141 ttcgttctgg atatttaaga aaagctgaac aatataagcg attagaattt aatttgagtt
    10201 ttgcactaga tgatattgaa agcacagcaa aggacgtaca aactgcaaaa tctagtgcta
    10261 ataaggacag tgtaactgtt aagggaaagg cgcccaatac gttatatatt gaaaaaagaa
    10321 atttgatgaa acaaaagctt gaaatgttgg gtgaagatat cgataaaaat aaagaatccc
    10381 tccaaaaagc taaggaaatt gctggcgaaa aggcaagtga atattttaat aaagcaatga
    10441 attaatattg aggtgaagat atgggtggat ataaaggtat taaagcagat ggtggcaagg
    10501 ttgatcaagc gaaacaatta gcggcaaaaa cagctaaaga tattgaagca tgtcaaaagc
    10561 aaacgcaaca gctcgctgag tatatcgaag gtagtgattg ggaaggacag ttcgccaata
    10621 aggtgaaaga tgtgttactc attatggcaa agtttcaaga agaattagta caaccgatgg
    10681 ctgaccatca aaaagcaatt gataacttaa gtcaaaatct agcgaaatac gatacattat
    10741 caattaagca agggcttgat agggtgaacc catgatgaaa gatgttaagc gaatagatta
    10801 tttttcttac gaagaattaa caattttagg tggtagtaaa ttgcctctcg taaattttga
    10861 attgtttgat ccatcaaatt ttgaagaagc taaagctgct ttaattgaaa aggaattagt
    10921 aacagagaat gacaagttaa ctgatgcagg ttttaaagtg gctacattag tcagagagta
    10981 tattagcgcc attgtaaata ttcgaattaa tgatatgtat tttgcaccat ttagctatga
    11041 aaaagatgaa tatattttgt taagccggtt taaaaataat gggtttcaaa tacgaattat
    11101 caataaagac attgcatggt ggtcgattgt acaatcatat cctttattga tgagacaaga
    11161 aaagtccaat gattgggact ttaaacaaat tgacgatgaa acattggaga acttaaataa
    11221 tgaaagtatc gatacgattg ggcgtgtttt agaaattgaa atatacaatc atcaaggtga
    11281 ccctcaacaa agtttatata acatttatga acaaaatgat ttgttattca ttcgataccc
    11341 attaaaagat aaagtgctga atgttcatat tggtgtcatt aatacattta tacgagaatt
    11401 atttggattc gatactgatg aaaatcatat taataaagca gaggagtaat gacgttgagt
    11461 ggaaaaatta gtgttaaagc tgaaacgatt gcacatgttg taaaagaatt ggaaagcata
    11521 agtcaaaagt atgatgaaat agctcaaaac tttggaaaaa tagcgcaatt aaattactac
    11581 agtagtgaaa aagctgcaca ttctatggaa aatggctata gtagtgctgc aacagtcatt
    11641 agtggtctca aaggtccatt gagtacactc ggtggtggcg tcatgaattc agcacaaaag
    11701 ttctttgaag cagatgaaca ttggggtacg gaatttgcca agctttacta taatattgag
    11761 ggataggtgc atgacatgac aaaagatatt gaatatctaa cagctgatta tgacaatgaa
    11821 aagtcatcta tccaaagtgt aatagatgca atagagggtc aagacttctt agatgtagat
    11881 acaacaatgg atgatgcggt aagcgatgtc agttctttag acgaagatgg cgcaatatca
    11941 ttaacaagta gtgtagtagg tccacaagga tctaaattaa tggggtatta tcaaaatgag
    12001 ttatatgatt atgcatctca attagattcg aaaatgaaag aaattattga cacgccattt
    12061 atagaagata tagataaagc attcaaaggt ataacgaatg ttaaattgga aaatatacta
    12121 attaaaaatg gtggtggaca tggtagagat acctatgggg cttctgggaa aattgcaaag
    12181 ggagatgcca agaaaagtga cagcgatgtt tatagcatcg atgaaatatt aaaatcggat
    12241 caagaatttg taaaagtaat tgatcagcat tacaaagaaa tgaaaaaaga agataagaaa
    12301 ttatctaaaa gtgattttga aaaaatgatg actcagggcg cttcttgtga ttacatgaca
    12361 gtagctgaag cggaagagct agaggagcaa aagaaaaaag aagaagctat agagattgca
    12421 gcactagctg gtatggtagt tttatcttgt attaatcctg ttgctggagc agtagctatt
    12481 ggtgcttatt ccgcttattc agcagcaaat gcagccacag gaaaaaatat tgtaactgga
    12541 agaaagctat ctaaagaaga acgaatcatg gaaggacttt cgcttattcc attgccaggt
    12601 atgggcttcc tcaaaggtgc tgggaaaagt ttaatgaaat taggcttcaa aggcggagaa
    12661 aaatttgcag ttaaaacagg attgcaaaag acaatgcaac aagcagttag tcgtatttca
    12721 cctaaaatgg gaatgatgaa aaacagtgtg ttgaatcaat ctcgtaactt tgctcaaaat
    12781 actcatgttg gacaaatgct gagtaacatg cgtggtcaag caactcatac tgttcaacaa
    12841 agtagaaatt ggattggaca acaagcacaa aatgtcaaac gaatagtgaa taatggactt
    12901 gataaagaaa tagcacatcc atttaaacaa caacttgcac cagcgggaat gggtggtata
    12961 aaatttgctg aaacaactac tttgagaaac atgggtcaaa acataaagcg tgctgttaca
    13021 ccacaaaatc acgtgacaca tggtccaaaa gatagtatgg tgagaagtga aggcaaacat
    13081 agtataagta gccatgaaat gaattcatca aagtatgttg aatcaccaaa ctacaccaag
    13141 gttgaattcg gagaacacta tgcaagactt agacctaaga aactaaaggc gaatattgaa
    13201 tacacaacac ctactggtca catatatcga accgatcata aaggtcgcat aaaagaagtt
    13261 tatgtagaca atctctctct aaaagatggg gatcgtaata gccatgcaca aagaactgtg
    13321 gggggagagg atagattacc agacgatgat ggaggtcact taatcgctag aatgtttggt
    13381 ggttcaaaag acattgataa ccttgtggca caaagtaaat ttatcaaccg tccatttaag
    13441 gagaaaggtc attggtataa tcttgagaaa gagtggcaag agttcttaaa ctctgggaaa
    13501 gaggtgaaaa atattaaaat ggaagtaaaa tatagcggta atagtcaaag accgactata
    13561 tttaaagttg aatatgaaat taatggtgaa agaaatatta gaagaatatt aaataagtag
    13621 aggtgccaac atgacatttg aagagaagct tagcaaaata tacaatgaaa ttgcgaatga
    13681 gattagcagt atgataccgg tagagtggga aaaagtatat acaatggctt atatagatga
    13741 tggaggaggt gaagtattct ttaattatac taaaccaggt agtgatgact tgaattatta
    13801 caccaatata cctaaggagt ataacatttc tgtgcaagta tttgatgatt tatggatgga
    13861 tttatatgat ttatttgagg agttaagaga tttatttaaa gaagaagatt tagaaccatg
    13921 gacatcatgc gaatttgatt ttacaagaga aggtgaatta aaagtttcat ttgattatat
    13981 tgattggata aattcagaat ttggtcaaat aggtcgacaa aattactata agtatagaaa
    14041 atttggaatt ttaccagaaa cggaatatga aattaataaa gttaaagaaa tcgagcaata
    14101 tattaaagag ctagaagaat aaactatctt aatgtaagac taaacaataa agctttgttt
    14161 agtcttttta gcgtttaagt aaaaa
//
LOCUS       DAXWFK010000058        13848 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.058, whole genome shotgun sequence.
ACCESSION   DAXWFK010000058 DAXWFK010000000
VERSION     DAXWFK010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13848
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.058"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(30..857)
                     /locus_tag="ACGEXS_001331"
     CDS             complement(30..857)
                     /locus_tag="ACGEXS_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFF8012349.1"
                     /translation="MWKWEAENDAKGVVVIAHNILEHTGRYAYVITMLRRNGYHVIMG
                     DLPGQGQTSRANKGQIENFQTYHESLLDWLKIANEYKIPTYVLGVGLGGLILLNLLEK
                     VELPIEGMMLISPMLELQKNGKNRKDKLVSNIGKISKDTRFNVGVEPKDLTRNLEIVE
                     ETVNDGLMLKKATYHWYNTINETMKDTMAHIHDIQPMPTLLMYGTKDLIVDTRAIDEF
                     KEKYQTPELYFKAWQGFYHEVHNEPERDEVMRYILTFLNNSVNTMGFIVEDDEIVEI"
     gene            complement(860..979)
                     /locus_tag="ACGEXS_001332"
     CDS             complement(860..979)
                     /locus_tag="ACGEXS_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012350.1"
                     /translation="MMLISFIYNNMDNNNTINRNLHSEFAIIMLLKINEKGAV"
     gene            1091..2092
                     /locus_tag="ACGEXS_001333"
     CDS             1091..2092
                     /locus_tag="ACGEXS_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HFF8012351.1"
                     /translation="MALLKNFFIGLSNNSFLNNAAKKVGPRLGANKVVAGNTIPELIN
                     TIEYLNDKNIAVTVDNLGEFVGTVEESNHAKEQILTIMDALHQHGVKAHMSVKLSQLG
                     AEFDLELAYQNLREILLKANTYNNMHINIDTEKYASLQQIVQVLDRLKGEFRNVGTVI
                     QAYLYDSHELVDKYQDLRLRLVKGAYKENESIAFQSKEDVDANYIKIIEQRLLNARNF
                     TSIATHDHRIINHVKQFMKENHIEKDRMEFQMLYGFRSELAEEIANEGYNFTIYVPYG
                     DDWFAYFMRRLAERPQNLSLAVKEFVKPAGLKRVGIIAALGATVMLGLSTIKKLCRK"
     gene            complement(2214..2678)
                     /gene="ribE"
                     /locus_tag="ACGEXS_001334"
     CDS             complement(2214..2678)
                     /gene="ribE"
                     /locus_tag="ACGEXS_001334"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFF8012352.1"
                     /translation="MNFEGKLIGKDLKVAIVVSRFNDFITGRLLEGAKDTLIRHDVNE
                     DNIDVAFVPGAFEIPLVAKKLASSGNYDAVITLGCVIRGATSHYDYVCNEVAKGVSKV
                     NDQTNVPVIFGILTTESIEQAVERAGTKAGNKGAEAAVSAIEMANLLKSIKA"
     gene            complement(2691..3872)
                     /gene="ribB"
                     /locus_tag="ACGEXS_001335"
     CDS             complement(2691..3872)
                     /gene="ribB"
                     /locus_tag="ACGEXS_001335"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFF8012353.1"
                     /translation="MQFDNIDSALMALKNGEPIIVVDDENRENEGDLVAVTEWMNDNT
                     INFMAKEARGLICAPVSKDIAQRLDLVQMVDDNSDIFGTQFTVSIDHVDTTTGISAYE
                     RTLTAKKLIDPSSEAKDFNRPGHLFPLVAQDKGVLARNGHTEAAVDLAKLTGAKPAGV
                     ICEIMNDDGTMAKGQDLQKFKEKHQLKMITIDDLIEYRKKLEPEIEFKAKVKMPTDFG
                     TFDMYGFKATYTDEEIVVLTKGAIRQHENVRLHSACLTGDIFHSQRCDCGAQLESSMK
                     YINEHGGMIIYLPQEGRGIGLLNKLRAYELIEQGYDTVTANLALGFDEDLRDYHIAAQ
                     ILKYFNIEHINLLSNNPSKFEGLKQYGIDIAERIEVIVPETVHNHDYMETKKIKMGHL
                     I"
     gene            complement(3883..4515)
                     /locus_tag="ACGEXS_001336"
     CDS             complement(3883..4515)
                     /locus_tag="ACGEXS_001336"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFF8012354.1"
                     /translation="MFTGIVEEIGVVKSVQIRQSVRTIEIEAHKITADMHIGDSISVN
                     GACLTVIDFNQTSFTVQVIKGTENKTYLADVKRQSEVNLERAMSGNGRFGGHFVLGHV
                     DELGTVSKINETANAKIITIQCSQHINNQLVKQGSITVDGVSLTVFDKHDNSFDIHLI
                     PETRRSTILSSKKLGDKVHLETDVLFKYVENILNKDKDQLSVDKLRAFGF"
     gene            complement(4522..5565)
                     /gene="ribD"
                     /locus_tag="ACGEXS_001337"
     CDS             complement(4522..5565)
                     /gene="ribD"
                     /locus_tag="ACGEXS_001337"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFF8012355.1"
                     /translation="MSQFMDYAIQLANMVQGQTGVNPPVGAVVVKEGRIVGIGAHLRK
                     GDKHAEVQALDMAQQNAEGATIYITLEPCSHFGSTPPCVNKIIDCKIAKVVYATKDNS
                     LDTHGDETLRAHGIEVECVDDERASQLYQDFFKAKAKQLPQITVKVSASLDGKQANDN
                     GQSQWITNKEVKQDVYKLRHRHDAVLTGRRTVELDDPQYTTRIQDGKNPIKVILSKSG
                     NIHFNQQIYQDESTPIWIYTENPNLTTNQTHIEIIYLKSCDLTTILHNLYKRGVGTLL
                     VEAGPTTTSEFLQSNYIDEFILYYAPKLIGGSGNYQFYQTNDVIEIPDANQFEIVHSE
                     LLNQNVKLTLRKK"
     regulatory      complement(5692..5827)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(6046..7548)
                     /locus_tag="ACGEXS_001338"
     CDS             complement(6046..7548)
                     /locus_tag="ACGEXS_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFF8012356.1"
                     /translation="MRVAIIGMGTAGVSVLRELVKHPKFNQLDIDLYDDKVNMGQGVP
                     FQNDSSELLINMPSKKMSLNLDDETEFWKWYKQQTDFNFDEPAYLPRFVFGHYMKSYL
                     SMFTKKYPNISTNYNKVQEIYTNSNIDETNLTYYICTTNSGQSWQAYDYVFLTCGTFA
                     YHDPYNLKGKKGYIATPYPTYNTLDEVNELDDIAIIGTGLASLDVVRYVAAHHPKLPI
                     TMTSRSAHLPSVRGTMIDVTFKYLTKDKLNDIKKHHFGNAPLDTLVSLFLKECAEYDI
                     DFKKLVHRRTGNHIADLKYDLARPTEMGIFQSMIEHLKENLNWIWNSLSIEDQHQFNQ
                     KYSKMIQLNSNPMPPRTAELIIELIENKSLILKKDLEDVKHDGKLYYFSYKNQESVDI
                     YNVVINATGAKSHLNELDQDDQLIRNLENRQIVQAHPMGGIQIIPETNQVISPRFGTL
                     TNMIAIGQMTNGVNKLRNGVKMIVEQVAHTVSQLYDALESNEQQQRSDNQ"
     gene            8071..8385
                     /locus_tag="ACGEXS_001339"
     CDS             8071..8385
                     /locus_tag="ACGEXS_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFF8012357.1"
                     /translation="MTYKELATFLKVLSDSSRLEILDLLSCGELCACDLLAHFQFSQP
                     TLSYHMKALVKTNLVTTRKIGNKHLYQLNHNIFESVINNLSKVHTSNQRCICHNLNTG
                     EC"
     gene            8385..9677
                     /gene="arsB"
                     /locus_tag="ACGEXS_001340"
     CDS             8385..9677
                     /gene="arsB"
                     /locus_tag="ACGEXS_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015105 - arsenite transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015700 - arsenite transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter membrane subunit
                     ArsB"
                     /protein_id="HFF8012358.1"
                     /translation="MMTTLATLIFLVTLLFVLWQPKGLDIGITALTGAFIAVITGVVS
                     FSDVFEVTGIVWNATLTFVSVILISLILDKVGLFEWSAIHMLHASKGNGLKMFVYIIL
                     LGAIVAAFFANDGAALILTPIVLAMVKNIGFSKRAIFPFIIASGFIADTTSLPLIVSN
                     LVNIISADYFHVGFVRYFSRMIIPNLFSLLASIIVLWLYFRKAIPKTFDDNNIKHPKD
                     AINDLKLFKISWIVLVILLFGYLISEFTKIPVSIFTGIIAFIFLMLARKSNAVNIKQV
                     IKGAPWNIVLFSIGMYIVVFGLRNAGITLILAKILEYISNYGLFSTILGMGFISAFLS
                     SIMNNMPTVLIDAIAIGQSNVHGMLKEGLIYANVIGSDLGPKITPIGSLATLLWLHVL
                     TQKDVKISWGTYFKTGIIITIPVLFITLIGLYLTLIIF"
     gene            9695..10090
                     /gene="arsC"
                     /locus_tag="ACGEXS_001341"
     CDS             9695..10090
                     /gene="arsC"
                     /locus_tag="ACGEXS_001341"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HFF8012359.1"
                     /translation="MTKKTIYFICTGNSCRSQMAEGWAKQILADDWNVYSAGIETHGV
                     NPKAIEAMKEVGIDISNHTSDLIDNNIIKNSNLVVTLCSDADVNCPSLPTNVKKEHWG
                     FDDPAGKPWSEFQRVRDEIKIAIENFKSR"
     gene            complement(10385..11239)
                     /locus_tag="ACGEXS_001342"
     CDS             complement(10385..11239)
                     /locus_tag="ACGEXS_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HFF8012360.1"
                     /translation="MNKHKKGSIFGIIGLVVIFAVVSFLFFSMISDQIFFKHVKSDIK
                     IEKLNVTLNDAAKKQINNYTSQQVSNKKNDAWRDASATEIKSAMDSGTFIDNEKQKYQ
                     FLDLSKYQGIDKNRIKRMLVDRPTLLKHTDDFLKAAKDKHVNEVYLISHALLETGAVK
                     SELANGVEIDGKKYYNFYGVGALDKDPIKTGAEYAKKHGWDTPEKAISGGADFIHKHF
                     LSSTDQNTLYSMRWNPKNPGEHQYATDIKWAESNATIIADFYKNMKTEGKYFKYFVYK
                     DDSKHLNK"
     gene            complement(11515..11739)
                     /locus_tag="ACGEXS_001343"
     CDS             complement(11515..11739)
                     /locus_tag="ACGEXS_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012361.1"
                     /translation="MDYAHLNLEHFFARNDDLDVIRDRADFVMINNFTNEMMYRDGQI
                     EGTIDLNQYYYKNRSNAASFIMMDYKKETK"
     gene            11938..12408
                     /gene="sigS"
                     /locus_tag="ACGEXS_001344"
     CDS             11938..12408
                     /gene="sigS"
                     /locus_tag="ACGEXS_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigS"
                     /protein_id="HFF8012362.1"
                     /translation="MKFNDVYNKHHKIIHHLLKKYNISYNYDEYYQLLLIKMWQLSQI
                     YKPSSKQSLSSFLFTRLNFYLIDLFRQQNQLKDVILCENNSPTLTEQPTYFNEHDLRL
                     QDIFKLLNQRERLWLKLYLEGYKQFEIAEIMSLSLSTIKLIKMSVKRKCQHNFN"
     gene            12521..12964
                     /locus_tag="ACGEXS_001345"
     CDS             12521..12964
                     /locus_tag="ACGEXS_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HFF8012363.1"
                     /translation="MQDNSTKYLLYIQTATSNHLETNCVFLHCDYILKVPINKLVSYY
                     AKLHLSSQSVLIETAKNILNINKLVPIYINPKTILFPLKHKRAPIQIYINAHYIVGMT
                     AIENSTLIHFQEGIQLEVDEPFSLVSKKCHESLALKHFIENTISN"
     gene            complement(12951..13394)
                     /locus_tag="ACGEXS_001346"
     CDS             complement(12951..13394)
                     /locus_tag="ACGEXS_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012364.1"
                     /translation="MNKASFDKKVKKQLWFLNKKEKQALDQRLSSISDDDSVNLNKPV
                     TFANAYLRQNVFRNKETKSYSMFVTLVVMMFAYVALLGLFLFGLITSLSGVQFFVSPK
                     VDLSTTVVILTIIGAILLMFASIYFIKIVTSYFTKKLLEIKFNSK"
     gene            13690..>13848
                     /gene="sdpA"
                     /locus_tag="ACGEXS_001347"
     CDS             13690..>13848
                     /gene="sdpA"
                     /locus_tag="ACGEXS_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic endopeptidase
                     SdpA"
                     /protein_id="HFF8012365.1"
                     /translation="MQKFKDFFYDDLSVTRGNYFLTLMAAFFITIILFIGIVVSEVHL
                     LYSMLIVLV"
BASE COUNT         4528 a         2396 c         1909 g         5015 t
ORIGIN      
        1 gggatttatg tcccaacctt tttagaatat taaatttcta caatttcgtc atcttcaaca
       61 ataaagccca ttgtattgac gctgttattt aagaaagtca gaatataacg cattacttca
      121 tcacgttctg gctcattgtg aacctcgtgg taaaaacctt gccaagcttt aaaatataat
      181 tcaggtgttt gatatttttc tttaaactca tcaattgccc tagtatcaac aattaaatcc
      241 ttcgttccat acattaatag cgttggcatt ggttgaatgt catgaatatg agccatcgta
      301 tctttcatcg tctcattaat tgtattatac caatgatacg ttgctttttt taacattaaa
      361 ccatcgttaa ctgtttcttc aacaatttct aaattacgtg ttaaatcttt tggttctaca
      421 ccaacattaa aacgtgtgtc ttttgaaatt ttacctatat ttgaaacaag tttatcttta
      481 cgattttttc cattcttttg aagttctagc ataggagaaa ttaacatcat cccctcgatt
      541 ggcaattcta ctttttcaag taaatttaat aaaatcaaac cgccaagtcc tacccctaat
      601 acataagtag gaattttata ttcattagct atctttaacc agtctagcaa actttcgtga
      661 tacgtttgaa agttttcaat ttgtccttta ttagctcttg aagtttgacc ttgaccaggc
      721 aaatctccca taatcacatg atagccattt cttcttaaca tcgtaataac atatgcatat
      781 cttcccgtat gttctaatat attatgagca ataacaacga cgcctttcgc atcattttca
      841 gcttcccact tccacattat tatactgccc ctttttcatt aatcttcaat aacataatta
      901 tagcaaattc actatgtaga tttctattta tagtattatt gttgtccata ttattatata
      961 taaatgaaat caacatcaat aatagtgtaa ttatacataa ttatttttga ttgtttttga
     1021 tgaaaacgct ttctcaaata tttttttcat gctaaactta ttgtaaacac aagggtttgg
     1081 aggagtagca atggcactat taaagaattt ttttatcgga ttatctaata atagtttttt
     1141 aaacaacgca gcaaaaaaag tgggcccacg tttgggcgcc aataaagtcg ttgccggaaa
     1201 tacaattcca gagttaatta atacaatcga atacttaaat gacaagaata tcgctgttac
     1261 ggtagacaat ttaggggaat ttgtcggtac agttgaagaa agtaatcatg ctaaagaaca
     1321 aattttaaca attatggacg cgcttcatca acatggcgta aaggcacata tgtctgttaa
     1381 attgagtcag ttaggtgcag aattcgactt agaattagct taccaaaatt taagagagat
     1441 tttacttaaa gcaaatactt acaacaatat gcatataaat attgatactg aaaaatatgc
     1501 tagcctgcaa caaattgttc aagttttaga tcgcttaaaa ggcgaattta gaaatgttgg
     1561 tactgtaatt caagcatatt tatacgatag ccacgaatta gttgataagt accaagattt
     1621 acgattacgt ttggttaaag gtgcatataa agaaaacgaa tcaattgcat ttcaatctaa
     1681 ggaagacgta gatgcaaatt acatcaaaat aattgaacaa cgtttgttaa acgcacgcaa
     1741 tttcacttca attgcaacac atgaccatcg catcattaat catgtaaaac aatttatgaa
     1801 agaaaatcac attgaaaaag atcgtatgga attccaaatg ctctatggtt ttagatcaga
     1861 gttagcagaa gaaatcgcaa atgaaggcta taatttcact atttatgtac cttatggcga
     1921 tgattggttt gcgtatttta tgagaagatt agcagaacgc ccacaaaacc tatctcttgc
     1981 tgtaaaagaa tttgtgaaac ctgctggctt aaaacgtgtt ggcataattg cagctttagg
     2041 agctacagtt atgttaggtt taagtacaat taaaaaatta tgccgtaaat agagcaagac
     2101 ataaacaata atttaggagt ctggaacaat aatcaatgtt ctaggctcct aaatgttata
     2161 ttggcagttc tgaaacattt ataaatcttt gattatgaac tcacaacagt gctctatgct
     2221 tttatagatt ttaataaatt agccatttca attgcactta ctgctgcttc agcaccttta
     2281 ttgccagctt tcgtacctgc tctttccaca gcttgttcaa tactttcagt cgttaaaata
     2341 ccaaatatga ctggtacatt agtttgatca ttcactttag aaacaccttt cgcgacttca
     2401 ttacaaacat aatcataatg agacgtagca ccgcgaatta cgcatcctaa tgtaattact
     2461 gcatcataat ttcctgatga ggctaatttt ttagctacta aaggaatttc aaacgcacct
     2521 ggcacaaatg ctacatcaat attgtcttca ttaacatcat gtcgaatcaa agtatctttt
     2581 gcaccttcaa gtaatcttcc agtgataaaa tcattaaatc gactaactac gattgcaact
     2641 ttcaaatctt ttccaattaa tttaccttca aaattcatgt taaaatcctc ctatattaaa
     2701 tgacccattt ttattttttt cgtttccata taatcatgat tatgtaccgt ttctggtacg
     2761 ataacttcaa ttctttctgc aatatcaatg ccatattgtt ttaatccctc aaatttactt
     2821 ggattattac ttaataaatt gatatgttcg atgttaaaat attttaaaat ctgtgcagca
     2881 atatgataat ctcgcaaatc ttcatcaaaa cctaatgcta aatttgcagt tactgtatca
     2941 tatccttgct caattaattc atatgcgcgt aatttgttta acaatcctat gccacgacct
     3001 tcttgaggta gataaataat catgccacca tgttcattga tatacttcat agacgattca
     3061 agttgagcac cacaatcaca acgttgacta tggaaaatat cgcctgtaag gcacgcagaa
     3121 tgtaagcgta cattttcatg ttgtcgaatt gcaccttttg tcagtacaac tatctcttca
     3181 tctgtgtatg tcgctttaaa accatacata tcaaatgttc cgaaatctgt aggcattttc
     3241 acttttgcct taaattcaat ttctggttct aattttttac gatattcaat taaatcatca
     3301 atcgtaatca tctttaattg atgtttttct ttaaactttt gtaaatcttg tcctttcgcc
     3361 atcgtgccgt catcattcat aatctcacaa atgacaccag cgggcttggc accagtaagt
     3421 ttagctaaat caacagccgc ttctgtgtgt ccatttctag ctaatacgcc tttatcttgt
     3481 gctactaatg gaaataaatg accaggacga ttaaaatctt tagcttcact actaggatca
     3541 atgagctttt tggcagtcaa tgtacgttca taagcactaa ttcctgttgt tgtatctaca
     3601 tgatcaatac tcactgtaaa ttgcgtacca aagatgtcgg agttatcatc aaccatttgt
     3661 accaaatcca aacgttgtgc aatatcttta gacactggtg cgcatattaa tccccttgct
     3721 tctttcgcca taaaattaat ggtattatcg ttcatccatt cagtaaccgc tactaaatca
     3781 ccttcatttt cacgattctc atcatctact acaataattg gttctccatt ttttaaagcc
     3841 attaaagcac tgtcaatatt atcgaattgc atgctacccc tcctaaaaac caaatgctct
     3901 taatttatct acagataatt ggtctttatc tttatttaaa atattttcaa catatttaaa
     3961 caaaacgtct gtttctaaat gtactttatc tcctaatttt ttggatgata aaatcgttga
     4021 acgcctcgtt tctggaataa gatgaatgtc aaaactgtta tcatgcttat caaataccgt
     4081 tagactaaca ccatccacag taatagaccc ttgcttaact aactgattat taatatgttg
     4141 actacattga atcgtaataa tttttgcatt ggctgtttca tttatttttg aaactgttcc
     4201 tagttcatct acatgaccga ggacaaaatg tccaccaaac ctaccgttac cactcatggc
     4261 acgctctaaa tttacttctg attgtcgctt aacatctgct aaataggttt tattttcagt
     4321 gcctttaatt acttgaacag taaaagatgt ctgattaaaa tcaatcactg ttaaacatgc
     4381 accattaaca ctgatggaat caccaatatg catatctgcc gtaatcttat gtgcttcaat
     4441 ttcaatcgtc ctgactgatt gacgaatttg aacactttta acgacaccta tttcttcaac
     4501 gatgccagta aacatgcatc atcacttctt tcgtaaagtt aatttaacat tttgatttaa
     4561 taactcggaa tgaacaattt caaattggtt cgcatctggt atctcaatca catcatttgt
     4621 ttgataaaat tgataatttc cagatccgcc aattaatttc ggggcataat agagaataaa
     4681 ttcatctata taattagatt ggagaaattc tgaagtagtg gttggacctg cctcgactag
     4741 caaagttcca actcctcttt tatataaatt gtgaagaatt gttgttaaat cgcaagactt
     4801 caagtaaata atttcaatat gtgtttgatt ggttgttaaa tttggatttt cagtatatat
     4861 ccaaattggt gttgattcat cttgataaat ttgctgatta aaatgaatat tcccagactt
     4921 agacaatatt acttttatag ggttttttcc atcttgaata cgtgtagtat attgtggatc
     4981 atctaattca actgtacgtc ttccagttaa cactgcgtcg tgtcgatgtc ttaacttata
     5041 gacatcttgt ttaacctctt tgttagtaat ccattgactt tgtccattat cattcgcttg
     5101 tttaccatct aaacttgcag atactttcac tgtaatttgt ggcagttgct ttgcttttgc
     5161 tttaaaaaag tcttggtata attgtgatgc ccgttcatca tcaacgcatt caacctcaat
     5221 accgtgagcc cgtaacgtct catcaccatg tgtgtctaac gaattgtctt ttgttgcgta
     5281 tactactttt gctatcttac aatcaattat tttgttaaca cagggtggtg ttgaaccaaa
     5341 atgactacat ggctctaacg taatataaat cgtcgcacct tcagcatttt gttgtgccat
     5401 atcaagtgct tgaacctccg catgcttgtc accttttctc aagtgtgcac caataccaac
     5461 aatcctacct tctttaacta caacagcgcc aacgggtgga ttaacacctg tttgaccttg
     5521 taccatattt gcaagttgaa tcgcataatc cataaattga ctcaaatgat cacctctata
     5581 aacaaaaatc ctcacatcat gaattaagat gcaaggagaa aaatttatcg ttaaataagc
     5641 ctatttgtac acatttttac aaatacgcta cattatcttt gtcgataatt aacattcttt
     5701 ctcccatcca gactttaact gtcggctcta gaatctcact agatcagcca ctaatatgaa
     5761 acatattagc aggtcgcagg ctttatttac tgccggttgg gaatttcacc ctgccccgaa
     5821 agaattatat atgaaattgt tatagattat ttgagtacgt agtatgtcaa ctacatttaa
     5881 aatgatacta tatgttttct gaaaaaacaa ttaatgacgg ttttaattta atataatctg
     5941 agtactatag gcatctcatt gatatgattc ttactaacag acattaaaat caaaccttca
     6001 attcgtctct atagagcgtt ctctttatta tcttctagtt acaaattatt gattgtcact
     6061 gcgctgttgt tgctcattcg attctaaagc atcatataat tgagatactg tatgcgcaac
     6121 ttgttctaca atcattttca caccgtttcg tagtttatta acaccgtttg tcatttgacc
     6181 tatcgcaatc atatttgtta atgttccaaa ccttggacta ataacttgat tggtttccgg
     6241 aatgatttgt atgcctccca ttgggtgtgc ttgtacaatt tgtctatttt caagatttct
     6301 aattaattga tcatcttgat ccaattcatt taaatgactt tttgcacctg tcgcgttaat
     6361 gacaacatta tatatgtcta ctgattcttg gtttttgtat gaaaaataat acaacttgcc
     6421 atcatgtttc acatcttcta aatctttttt caaaattaaa gacttatttt ctattaattc
     6481 aataattagt tcagcagttc ttggaggcat tggatttgaa tttaattgaa tcatctttga
     6541 gtatttttga ttaaattgat gttggtcttc aatacttaag ctattccata tccaatttaa
     6601 attctctttc aaatgttcaa tcatactttg gaaaatgccc atttctgttg gacgcgctaa
     6661 atcatacttc aaatctgcaa tatgatttcc tgtacgtcta tgtactaatt ttttaaaatc
     6721 aatgtcatat tcagcacatt cttttaaaaa taaagaaact aaagtatcaa gcggtgcatt
     6781 gccgaaatga tgttttttaa tgtcatttaa tttgtcttta gttaagtact tgaatgtcac
     6841 gtctatcatt gtacctctta cacttggtaa atgagcagaa cgactcgtca tagtaattgg
     6901 taattttgga tgatgagcag caacataacg gacaacatct aaactggcaa ggcctgtacc
     6961 aataatcgca atatcgtcca gttcatttac ttcgtctaac gtattatatg ttggataagg
     7021 cgtagcgata tatccttttt taccctttaa gttatatgga tcatggtagg caaatgtacc
     7081 acatgttaaa aatacataat cgtacgcttg ccatgattgt cctgaatttg tagtacatat
     7141 gtaataagtt aaattcgttt catcgatatt agaatttgta taaatctctt gaactttatt
     7201 ataattagtt gatatatttg gatatttttt cgtgaacata gataaataag atttcatata
     7261 atgtccgaat acaaatctcg gtaaatatgc aggttcatca aaattaaaat cagtttgttg
     7321 tttataccac ttccaaaatt cagtctcatc atctaaattt aaactcatct ttttcgaagg
     7381 catattaatt agcagctcag aactatcatt ttgaaatggt acgccctgtc ccatatttac
     7441 tttatcatcg tataaatcta tatctaattg attaaacttc gggtgcttaa ctaactctct
     7501 caatacactt acaccagcag ttcccatgcc tattattgct acacgcatat atcatccatc
     7561 cttttgtaat tcaaaatatt atatttaaac tattacctat aattatagca atttggtatt
     7621 tccaataaaa tttattaagg taagctatta tttcactttc acaaccatta aaccatttaa
     7681 aacttttcat ttaatatcaa ccttagtgtt tgtgttaaaa tatttttaac gaggtgaatt
     7741 aaattgaaat ttatagcaaa gatattaatc tcattagtaa ttctatttat gatttttagt
     7801 cgtaaaaaca aagcttctta atcaataccc ttgattacaa aataaattca actaaggtga
     7861 ttggccatac actcaagata tggctaatca cctttttcaa tgcagaacaa agagtatatt
     7921 ttgcaaaatc aatatctata atttatcttt aatacccttt taatactaaa accaaattta
     7981 aattacatag tcatatcgtg taatatacca aaggaacata gacattcatc tatatagatg
     8041 ataatctatt taacgtctag gaggtatgtt atgacgtata aagaactagc aacattttta
     8101 aaagttttat cagattcaag cagattagaa atactagatt tactttcttg tggagagtta
     8161 tgcgcttgtg atttgttagc acattttcaa ttctctcaac ctacacttag ctatcatatg
     8221 aaagcattag taaaaaccaa cttagttacg acacgaaaaa tcggaaataa acatttatac
     8281 cagctcaatc ataatatttt tgagtccgta attaataact tgtctaaggt tcatacctct
     8341 aaccaacgat gtatttgtca taaccttaat actggtgaat gctaatgatg acaactttag
     8401 cgacactcat ttttctagta actttattat ttgtattatg gcaacctaaa ggcctagata
     8461 ttggcattac cgcactaact ggtgccttta ttgctgttat tactggtgtt gtaagttttt
     8521 ccgatgtttt cgaagtaaca ggtattgttt ggaatgctac tttgactttt gtctcagtca
     8581 ttcttatttc attaatatta gataaagttg gattattcga atggtcagct attcacatgc
     8641 ttcatgcttc aaaaggcaat ggtttaaaaa tgttcgttta tatcatatta ttgggtgcca
     8701 ttgttgctgc atttttcgca aatgatggcg cagcgttaat cttaacgcct attgtattag
     8761 cgatggttaa aaatataggt tttagtaagc gggccatatt cccttttatt attgcgagtg
     8821 gttttatagc tgacacaact tctttacctt taatcgtgag caatctagtg aatattatat
     8881 ctgctgatta ttttcatgta ggattcgttc gatattttag tagaatgatt atacctaatt
     8941 tattctcgct tttagcaagt attatagtat tgtggttata ttttagaaaa gcgataccta
     9001 aaacgtttga tgataataat ataaagcatc ctaaagatgc cattaatgat ttaaagctat
     9061 ttaaaatttc atggattgtt ctagttatat tacttttcgg ctatctaatc agtgaattta
     9121 ctaaaattcc ggtatcaatt ttcactggaa tcattgcttt tatttttcta atgttggctc
     9181 gtaaatcaaa tgctgtaaat attaagcaag tcattaaggg cgcaccttgg aatatagtat
     9241 tattttcaat tggtatgtat atcgtcgtat tcggcttaag aaatgctggc attactttaa
     9301 tattggctaa aatattagaa tatatttcca attacggtct atttagcact attttgggaa
     9361 tgggcttcat ttcagcgttt ttatcatcaa taatgaataa tatgcctaca gttttaatag
     9421 atgcgattgc tattggtcaa tcaaatgtcc atggcatgtt aaaagaaggc ctaatttatg
     9481 cgaatgttat cggttctgat ttaggtccaa aaattacacc gataggctct ttagctacat
     9541 tactgtggtt acacgtctta acacaaaaag atgttaagat ttcttggggc acatacttta
     9601 aaactggtat catcattaca attccagtac tatttataac cctcataggg ttgtatctaa
     9661 cacttatcat attttaatta attaggagat tgttatgact aaaaaaacaa tttattttat
     9721 atgtacaggc aactcatgtc gaagtcaaat ggctgaaggt tgggctaaac aaatcttagc
     9781 ggatgattgg aatgtatatt ctgctggtat cgaaacacac ggtgttaatc ccaaagcgat
     9841 agaagctatg aaagaagtag gcattgatat atcaaatcat acatcagatt taatcgataa
     9901 taatattatt aaaaattcaa atttagttgt tacattatgt agtgatgcag acgtaaattg
     9961 cccttcttta ccaacaaatg ttaagaaaga acattgggga tttgatgatc ctgcaggcaa
    10021 gccttggtca gagttccaac gtgtaagaga tgaaattaaa atcgcaattg aaaatttcaa
    10081 atcacgatga gagtgggaca gaaatgataa aaaaacacta atgatttatt atgtagtggt
    10141 tctttatcat tagctataac taatgtgtac ttaaaaatag gaatacatga gtaaaactca
    10201 tgcataagaa atactaattt ctaaagaaaa agtaattctt tatcgatgtc ctgccccact
    10261 tgcattggtg ggattatcaa tcaatgtgtt ttctctaatg caaaaagacg ctttctatca
    10321 aaacaagata gaaagcgtct ttttagtatg tccgagtcat aaaaacaact cgtagcttat
    10381 caaattactt attcaaatgt ttactgtcat ctttatacac aaagtatttg aagtattttc
    10441 cttcagtctt catgttctta taaaagtcag cgataattgt tgcattactt tctgcccact
    10501 taatatctgt agcatattga tgttctcctg gattttttgg attccatctc atactataca
    10561 atgtattttg atctgtgctt gataagaagt gcttatgaat gaaatcagca ccgcctgaaa
    10621 tagctttttc aggtgtatcc caaccatgct ttttagcata ttctgcacct gttttaattg
    10681 ggtctttatc aagggctcct actccataga aattgtagta ctttttgcca tcaatttcga
    10741 ctccattagc taattcactt ttaactgcgc cagtttctaa taatgcatgt gaaattaaat
    10801 aaacttcgtt aacgtgctta tctttagcag cttttaagaa atcatccgta tgtttcaata
    10861 acgttggtct atctactaac atacgtttaa ttctattttt atcaatccct tgatactttg
    10921 ataaatctaa aaattgatat ttttgctttt cattatcgat aaaagtaccg ctatccattg
    10981 cacttttaat ttcagttgca gatgcatctc tccatgcatc attcttttta tttgatacct
    11041 gttgactcgt ataattattt atttgtttct ttgctgcatc gtttaatgta acatttaact
    11101 tttcaatctt aatgtcggat ttaacatgtt tgaaaaatat ctgatcggat atcattgaga
    11161 aaaataaaaa tgagacaaca gcaaatatga caacaagtcc tattattcca aaaatagaac
    11221 ctttcttgtg tttattcata tccacacctc ttaggtcatt gttgttatac attcagtttg
    11281 actacgataa taaagtttaa cattattaaa accgtttaac aattttatct atgcattgtt
    11341 acatattcat aaataaacag taatgtaaat gaaacgtttt aaaccattta tcaacattac
    11401 tttacgttga tctacgaatc ataaatgttt tatctttttg tataaccttt gaaaaccttt
    11461 atatcaataa tcttattatt cacgtattaa aaaacaaaaa attcttttgt tcgtttactt
    11521 agtttctttt ttataatcca tcataataaa acttgctgca tttgatctat ttttataata
    11581 gtactgattt aaatcaatcg tgccttcaat ttgaccatca cgatacatca tttcattagt
    11641 gaagttattt atcatcacga aatcagcgcg atctcttata acatctaaat cgtcgtttcg
    11701 tgcaaaaaaa tgttctaaat ttaaatgtgc gtaatccatt gttacctccc gattacaaat
    11761 tttgttctat ctactattta gcaaagtaat aatacatcat atcacattaa attaattact
    11821 tatgtcttat atttaacact taatttagcg attacgcaaa tgaatgattt ttctaaatat
    11881 tttattctaa ctttatccgg aaatatttgt tatacgtata acttggagaa tgactacttg
    11941 aaatttaatg acgtatacaa caaacaccac aaaatcatac accatctttt aaaaaaatat
    12001 aatattagct ataattatga tgagtattat caactactct tgataaaaat gtggcaattg
    12061 agtcagatat ataaaccctc aagcaagcaa tctttatcct cttttttatt cactcgatta
    12121 aatttttacc ttatcgattt attcagacaa caaaatcaat taaaagatgt cattttatgt
    12181 gagaataatt caccaacatt aactgaacaa ccaacatact ttaatgaaca tgaccttcgt
    12241 ttacaagata tcttcaagct tttaaatcaa agagaaagac tatggctcaa actatacctt
    12301 gaaggataca agcaatttga aattgctgaa atcatgtcat tatcgctttc aacgattaaa
    12361 ttaattaaga tgtccgttaa gcgtaaatgc caacataatt ttaattagtt tattgtgaaa
    12421 tttcggtcga tgtgttaatg atgttgaaac accctaacat cttcatataa atttatttta
    12481 accattaaat taacaactta ttaaacgagg agtactatat ttgcaagaca attctactaa
    12541 atatctactt tatatccaaa ctgctacttc aaaccatctc gaaacaaatt gtgtcttttt
    12601 acattgcgat tacattctta aagttccaat taacaaactc gtttcatatt atgcgaaatt
    12661 gcatttatca tcacaaagtg tgctaattga gactgcaaaa aacatactaa atattaataa
    12721 actggttcct atttacatca acccaaaaac tatacttttt ccgttaaaac ataaacgtgc
    12781 accgatacaa atttatatca atgcacatta tattgttggt atgactgcca tagagaattc
    12841 aacattaata cattttcaag aaggcattca gctagaagtt gatgagccat tttctctagt
    12901 ttcaaaaaaa tgtcatgaaa gtttagcttt gaagcacttc attgaaaata ctatttcgaa
    12961 ttaaatttaa tttctaaaag tttcttagta aagtatgatg tcacaatttt tataaaatat
    13021 atgctagcaa acattaataa aatcgctcca ataattgtta aaataacaac agttgtagat
    13081 aaatctacct taggactaac gaaaaattgt acgccagata gacttgtaat tagcccaaat
    13141 aaaaacaaac ctaataaagc aacataagca aacatcatca ctactaatgt aacaaacata
    13201 ctataacttt ttgtttcttt atttcgaaat acattttgtc ttaagtaagc attagcaaag
    13261 gtaacaggct tatttaagtt aacgctgtca tcatcagata tagaagacaa tcgttgatct
    13321 aatgcttgtt tctccttctt gttcaaaaac cataattgct ttttaacttt tttatcaaat
    13381 gatgctttat tcataattaa ggatttctct cctttataat attgacgtaa ttttaaacat
    13441 tagcgtcaat tgaaagtgta ttttatatat tttacagaaa tatatgtatc aaatctacaa
    13501 aaatccatat tacaatttca tttatggtga tttgacgcta aaatacagtc tgctaatttt
    13561 tcaaactaat gctttttagg taggtacaat gataaatttt aatcgtaaat atgtttcata
    13621 tattatgaat ctagccttaa tcatattgca ttcaaaataa ttttaaaaaa tgaaaagaaa
    13681 ggatttagca tgcaaaaatt caaagacttt ttttacgatg atttatcggt tacacgagga
    13741 aattattttt taactttaat ggcagcattt tttattacta tcattttatt tatcggcata
    13801 gttgtcagtg aagtacattt actttatagc atgctaattg tattagta
//
LOCUS       DAXWFK010000059        13681 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.059, whole genome shotgun sequence.
ACCESSION   DAXWFK010000059 DAXWFK010000000
VERSION     DAXWFK010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13681
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.059"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            469..1764
                     /gene="purB"
                     /locus_tag="ACGEXS_001348"
     CDS             469..1764
                     /gene="purB"
                     /locus_tag="ACGEXS_001348"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFF8012366.1"
                     /translation="MIERYSREEMSNIWTDQNRYEAWLEVEILACEAWSELGHIPKAD
                     VQKIRQNAKVNVERAQEIEQETRHDVVAFTRQVSETLGEERKWVHYGLTSTDVVDTAL
                     SFVIKQANDIIEKDLERFIDVLAEKAKNYKYTLMMGRTHGVHAEPTTFGVKMALWYTE
                     MQRNLQRFKQVREEIEVGKMSGAVGTFANIPPEIESYVCKHLGIGTAPVSTQTLQRDR
                     HAYYIATLALIATSLEKFAVEIRNLQKTETREVEEAFAKGQKGSSAMPHKRNPIGSEN
                     ITGISRVIRGYITTAYENVPLWHERDISHSSAERIMLPDVTIALDYALNRFTNIVDRL
                     TVFEDNMRNNIDKTFGLIFSQRVLLALINKGMVREEAYDKVQPKAMISWETKTPFREL
                     IEQDESITSVLTKEELDECFDPKHHLNQVDTIFERAGLA"
     gene            1873..2175
                     /locus_tag="ACGEXS_001349"
     CDS             1873..2175
                     /locus_tag="ACGEXS_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HFF8012367.1"
                     /translation="MQIEKLRGAALDELFDAILTLENREECYQFFDDLCTVNEIQSLS
                     QRLQVAKMIKQGYTYATIEQESGASTATISRVKRSLQWGNDAYTMILDRMNIETNE"
     gene            2347..3039
                     /locus_tag="ACGEXS_001350"
     CDS             2347..3039
                     /locus_tag="ACGEXS_001350"
                     /EC_number="2.5.1.n9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenylglyceryl phosphate synthase"
                     /protein_id="HFF8012368.1"
                     /translation="MYDIKKWRHIFKLDPAKHISDDDLDAICMSQTDAIMIGGTDDVT
                     EDNVIHLMSRVRRYPLPLVLEISNIESVMPGFDFYFVPTVLNSTDVVFHNGTLLEALK
                     TYGHSIDFEEVIFEGYVVCNADSKVAKHTKANTDLTTEDLEAYAQMVNHMYRLPVMYI
                     EYSGIYGDVSKVQAVSEHLTETQLFYGGGISSEQQATEMAAIADTIIVGDIIYKDIKK
                     ALKTVKIKESSK"
     gene            3036..5228
                     /gene="pcrA"
                     /locus_tag="ACGEXS_001351"
     CDS             3036..5228
                     /gene="pcrA"
                     /locus_tag="ACGEXS_001351"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFF8012369.1"
                     /translation="MNALLNHMNTEQSEAVKTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     LDEKDVSPYNVLAITFTNKAAREMKERVQKLVGDQAEVIWMSTFHSMCVRILRRDADR
                     IGIERNFTIIDPTDQKSVIKDVLKNENIDSKKFEPRMFIGAISNLKNELKTPADAQKE
                     ATDYHSQMVATVYSGYQRQLSRNEALDFDDLIMTTINLFERVPEVLEYYQNKFQYIHV
                     DEYQDTNKAQYTLVKLLASKFKNLCVVGDSDQSIYGWRGADIQNILSFEKDYPEANTI
                     FLEQNYRSTKTILNAANEVIKNNSERKPKGLWTANTNGEKIHYYEAMTERDEAEFVIR
                     EIMKHQRNGKKYQDMAILYRTNAQSRVLEETFMKSNMPYTMVGGQKFYDRKEIKDLLS
                     YLRIIANSNDDISLQRIINVPKRGVGPSSVEKVQNYALQNNISMFDALGEADFIGLSK
                     KVTQECLNFYELIQSLIKEQEFLEIHEIVDEVLQKSGYREMLERENTLESRSRLENID
                     EFMSVPKDYEENTPLEEQSLINFLTDLSLVADIDEADTENGVTLMTMHSAKGLEFPIV
                     FIMGMEESLFPHIRAIKSEDDHEMQEERRICYVAITRAEEVLYITHATSRMLFGRPQS
                     NMPSRFLKEIPESLLENHSSGKRQTIQPKAKPFAKRGFSQRTTSTKKQVLSSDWNVGD
                     KVMHKAWGEGMVSNVNEKNGSIELDIIFKSQGPKRLLAQFAPIEKKED"
     gene            5232..7235
                     /gene="ligA"
                     /locus_tag="ACGEXS_001352"
     CDS             5232..7235
                     /gene="ligA"
                     /locus_tag="ACGEXS_001352"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFF8012370.1"
                     /translation="MADLSSRVNELHDLLNQYSYEYYVEDNPSVPDSEYDKLLHELIK
                     IEEEHPEYKTVDSPTVRVGGEAQASFNKVNHDTPMLSLGNAFNEDDLRKFDQRIREQI
                     GNVEYMCELKIDGLAVSLKYVDGYFVQGLTRGDGTTGEDITENLKTIHAIPLKMKEPL
                     NVEVRGEAYMPRRSFLRLNEEKEKNDEQLFANPRNAAAGSLRQLDSKLTAKRKLSVFI
                     YSVNDFTDFNARSQSEALDELDKLGFTTNKNRARVNNIDGVLEYIEKWTSQRESLPYD
                     IDGIVIKVNDLDQQDEMGFTQKSPRWAIAYKFPAEEVVTKLLDIELSIGRTGVVTPTA
                     ILEPVKVAGTTVSRASLHNEDLIHDRDIRIGDSVVVKKAGDIIPEVVRSIPERRPEDA
                     VTYHMPTHCPSCGHELVRIEGEVALRCINPKCQAQLVEGLIHFVSRQAMNIDGLGTKI
                     IQQLYQSELIKDVADIFYLTEEDLLPLDRMGQKKVDNLLAAIQQAKDNSLENLLFGLG
                     IRHLGVKASQVLAEKYETIDRLLTVTEAELVEIHDIGDKVAQSVVTYLENEDIRALIQ
                     KLKDKHVNMIYKGIKTSDIEGHPEFSGKTIVLTGKLHQMTRNEASKWLASQGAKVTSS
                     VTKNTDVVIAGEDAGSKLTKAQSLGIEIWTEQQFVDKQNELNS"
     gene            7248..8447
                     /locus_tag="ACGEXS_001353"
     CDS             7248..8447
                     /locus_tag="ACGEXS_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CamS family sex pheromone protein"
                     /protein_id="HFF8012371.1"
                     /translation="MKRTLVLLITAIFILAACGNHKDDQAGKDNQKHNNSSNQVKEIA
                     TDKNVQGDNYRTLLPFKESQARGLLQDNMANSYNGGDFEDGLLNLSKEVFPTDKYLYQ
                     DGQFLDKKTINAYLNPKYTKREIDKMSEKDKKDKKANENLGLNPSHEGETDPEKIAEK
                     SPAYLSNILEQDFYGGGDTKGKNIKGMTIGLAMNSVYYYKKEKDGSTFSKKLDDSEVK
                     KQGKQMASEILSRLRENDDLKDIPIHFAIYKQSSEDSITPGEFITQATAEKSQTKLNE
                     WHNINEKSALLPSSTAADYDENLNNNFKQFNDNLQSYFSNFTQAVGKVKFVDKKPQRL
                     VVDLPIDYYGQAETIGITQYVTEQANKYFDKIDNYEIRIKDGNQPRALISKTKDDKEP
                     QVHIYSN"
     gene            complement(8536..10074)
                     /gene="putP"
                     /locus_tag="ACGEXS_001354"
     CDS             complement(8536..10074)
                     /gene="putP"
                     /locus_tag="ACGEXS_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFF8012372.1"
                     /translation="MLTMGTALSQQVDANWQTYIMIAVYFLILIVIGFYGYKQATGNL
                     SEYMLGGRSIGPYITALSAGASDMSGWMIMGLPGSVYSTGLSTMWITIGLTLGAYINY
                     FVVAPRLRVYTELAGDAITLPDFFKNRLNDKNNVLKIISGLIIVVFFTLYTHSGFVSG
                     GKLFESAFGLDYHFGLILVAFIVIFYTFFGGYLAVSITDFFQGVIMLIAMVMVPIVAM
                     MNLNGWGTFHDVAAMKPTNLNLFKGLSFIGIISLFSWGLGYFGQPHIIVRFMSIKSHK
                     MLPKARRLGISWMAVGLLGAVAVGLTGIAFVPAYHIKLEDPETLFIVMSQVLFHPLVG
                     GFLLAAILAAIMSTISSQLLVTSSSLTEDFYKLIRGEEKAKTHQKEFVMIGRLSVLVV
                     AIVAIAIAWNPNDTILNLVGNAWAGFGASFSPLVLFALYWKGLTRAGAVSGMVSGALV
                     VIVWIAWIKPLAHINEIFGLYEIIPGFIVSVIVTYVVSKLTKKPGAFVETDLNKVRDI
                     VREK"
     gene            10440..10742
                     /gene="gatC"
                     /locus_tag="ACGEXS_001355"
     CDS             10440..10742
                     /gene="gatC"
                     /locus_tag="ACGEXS_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFF8012373.1"
                     /translation="MTKVTREEVEHIANLARLQISPEETEEMANTLESILDFAKQNDS
                     ADTEGVEPTYHVLDLQNVLREDKAIKGIPQELALKNAKETEDGQFKVPTIMNEEDA"
     gene            10744..12201
                     /gene="gatA"
                     /locus_tag="ACGEXS_001356"
     CDS             10744..12201
                     /gene="gatA"
                     /locus_tag="ACGEXS_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFF8012374.1"
                     /translation="MSIRYESVENLLTLIKDKKIKPSDVVKDIYDAIEETDPTIKSFL
                     ALDKENAIKKAQELDELQAKDQMDGKLFGIPMGIKDNIITNGLETTCASKMLEGFVPI
                     YESTVMEKLHNENAVLIGKLNMDEFAMGGSTETSYFKKTVNPFDHKAVPGGSSGGSAA
                     AVAAGLVPFSLGSDTGGSIRQPAAYCGVVGMKPTYGRVSRFGLVAFASSLDQIGPLTR
                     NVKDNAIVLEAISGADVNDSTSAPVDDVDFTSEIGKDIKGLKVALPKEYLGEGVADDV
                     KEAVQNAVETLKSLGAVVEEVSLPNTKFGIPSYYVIASSEASSNLSRFDGIRYGYHSK
                     EAHSLEELYKMSRSEGFGKEVKRRIFLGTFALSSGYYDAYYKKSQKVRTLIKNDFDKV
                     FENYDVVVGPTAPTTAFNLGEEIDDPLTMYANDLLTTPVNLAGLPGISVPCGQSNGRP
                     IGLQFIGKPFDEKTLYRVAYQYETQYNLHDVYEKL"
     gene            12214..13641
                     /gene="gatB"
                     /locus_tag="ACGEXS_001357"
     CDS             12214..13641
                     /gene="gatB"
                     /locus_tag="ACGEXS_001357"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFF8012375.1"
                     /translation="MHFETVIGLEVHVELKTDSKMFSPSPAHFGAEPNSNTNVIDLAY
                     PGVLPVVNKRAVDWAMRAAMALNMEIATESKFDRKNYFYPDNPKAYQISQFDQPIGEN
                     GYIDIEVDGETKRIGITRLHMEEDAGKSTHKGEYSLVDLNRQGTPLIEIVSEPDIRSP
                     KEAYAYLEKLRSIIQYTGVSDVKMEEGSLRCDANISLRPYGQEKFGTKAELKNLNSFN
                     YVRKGLEYEEKRQEEELLNGGEIGQETRRFDESTGKTILMRVKEGSDDYRYFPEPDIV
                     PLYIDDAWKERVRQTIPELPDERKAKYVNELGLPAYDAHVLTLTKEMSDFFESTIEHG
                     ADVKLTSNWLMGGVNEYLNKNQVELLDTKLTPENLAGMIKLIEDGTMSSKIAKKVFPE
                     LAAKGGNAKQIMEDNGLVQISDEATLLKFVNEALDNNEQSVEDYKNGKGKAMGFLVGQ
                     IMKASKGQANPQLVNQLLKQELDKR"
BASE COUNT         5152 a         1999 c         2547 g         3983 t
ORIGIN      
        1 tattagaagc gaataatttt aaaacatatt atgaaatgta agaagaaata ttatgaaact
       61 aggaaaagta gattgttgaa aattccatac aataggagaa gtaaagtgaa taaattctgg
      121 tattactaaa gctattgatt gtcctaattg taataaaaag ttggatagtg tagttgaggg
      181 gagaaaaact aaaatgcaaa aatatattct caatggatca ttataaatac caattagatt
      241 aaagtattgc gaacatttac aattagaaat cagtattaat ctgccataga taattgtatt
      301 aatgtaaaaa ataaaataga atgagaatat acggaaattt tttatacatt tataaccaat
      361 tctgttaaac tgtgaagagt agagttaaaa acgaactttt tgtttatttt tattaaaaaa
      421 tgttctgttt ttgaaaattc gagtatacat acgataggag ttaaaaaaat gattgaacgc
      481 tattctagag aagaaatgtc taatatttgg actgatcaaa atcgctatga agcatggtta
      541 gaagtggaaa ttttagcatg tgaagcatgg agtgaattag gacatatacc gaaagctgac
      601 gtacaaaaaa ttcgtcaaaa cgcaaaggta aatgtcgaac gtgcacaaga aattgaacaa
      661 gaaacgcgcc atgatgttgt agcctttact agacaagttt ctgaaacgct aggtgaagaa
      721 cgtaagtggg tacattatgg tttaacttct actgatgttg tagatacagc tttaagtttc
      781 gttattaaac aagcaaatga cattattgaa aaagatttag aaagatttat cgacgtttta
      841 gctgaaaaag caaaaaatta taaatataca ttgatgatgg gacgtacaca tggagtgcat
      901 gcagaaccaa ctacatttgg tgtcaaaatg gcattgtggt acacggaaat gcaacgtaac
      961 ttacaacgtt tcaaacaagt aagagaagaa attgaagtag ggaaaatgag tggtgcagta
     1021 ggtacttttg ctaacattcc tcctgaaata gaaagttatg tgtgtaaaca tttaggtatt
     1081 ggcacagcgc ctgtatcaac gcaaacattg caacgcgatc gacatgcata ctatattgcg
     1141 acattagcat taatcgcgac atcactagaa aaatttgccg ttgaaatacg taaccttcaa
     1201 aaaaccgaaa cgagagaagt tgaagaagca tttgcaaaag gccaaaaagg ttcatctgca
     1261 atgcctcata aacgaaatcc aattggttct gaaaatatca caggtatttc aagagtcatt
     1321 cgtggttata ttactacagc ttatgagaat gtgccattat ggcatgaaag agatatttct
     1381 cattcttctg ctgagcgtat tatgttacca gatgttacaa tagcactaga ttatgcattg
     1441 aatcgtttca caaatattgt ggaccgttta actgtatttg aagataatat gcgtaataac
     1501 atagataaaa catttggtct gattttctca caacgtgtct tattagcatt aattaataaa
     1561 ggtatggttc gtgaagaagc atacgacaaa gtacaaccaa aagcgatgat atcttgggaa
     1621 acgaaaacac cgttccgtga attaatcgaa caagatgagt ctattacaag tgttttaaca
     1681 aaagaagaat tggatgaatg tttcgatcct aaacatcatt tgaatcaagt tgacactatt
     1741 ttcgaacgcg ctggattagc ataaaattaa ataaagtaac gattcagatt taataaaata
     1801 taaatcgcta cactttaata gattaatgcg ttaaaatgac ataaagaata aaattatagg
     1861 ggttgtttcg atatgcaaat tgaaaaatta cgaggtgcag cgttagatga attgtttgat
     1921 gcaatactaa cgttagaaaa tagagaagaa tgttaccaat ttttcgatga tttgtgtact
     1981 gtaaatgaaa ttcaatcact gtctcaaaga ttacaagttg ctaaaatgat taagcaaggt
     2041 tatacctatg caacgattga acaagaatct ggagcatcga ctgcaacgat ttctagagtg
     2101 aagcgttcat tacaatgggg taatgatgct tatacaatga ttttagatcg tatgaatatt
     2161 gaaacaaatg aataataaat gaacgataaa caatggttat ctatctgcac taataaggta
     2221 gataatcatt gttttttcac gaaaaaattt acagagtaaa agaacttaaa tttcatatta
     2281 agtctttaga actcgacact taaaaatgct ataatcatat gtatgttaaa aaaggagttt
     2341 cggaaaatgt atgacattaa aaaatggcgc catattttta aattagaccc agctaaacat
     2401 atttcagatg atgatttaga tgcgatttgt atgtctcaaa cagatgcaat tatgattggt
     2461 ggaactgatg acgttactga agataatgtc attcatttaa tgagcagagt aagaagatac
     2521 ccattgcctt tagtacttga aatatcaaac atcgaaagtg taatgcctgg ttttgatttt
     2581 tattttgtac ctacagtact gaacagtaca gatgttgtat ttcacaatgg tacattatta
     2641 gaagcgctta aaacatatgg acatagtata gattttgagg aagtaatatt tgaagggtat
     2701 gtcgtgtgca atgctgatag caaagtggca aaacatacca aagcaaatac agatttaaca
     2761 acagaagatt tagaagcata tgcccaaatg gtcaatcata tgtatcgatt accggttatg
     2821 tatatagagt atagtggcat ttatggcgac gtatcaaagg ttcaagctgt ctcagaacat
     2881 ctaacagaaa cgcaactttt ttatggtggc ggtatttcct cagaacaaca agcgacagag
     2941 atggcagcta ttgcagatac aattatcgtc ggtgatatta tttataaaga tattaaaaaa
     3001 gctttaaaaa cagtaaaaat aaaggagtct agtaaatgaa tgcgttatta aatcatatga
     3061 atacagagca aagtgaagct gtaaagacaa cagaaggacc attgttaatt atggcaggtg
     3121 ctggttcagg gaagacacgt gttttaacac atagaattgc ttatttatta gacgaaaaag
     3181 atgtctcacc atacaatgtt ttggctatta cttttacaaa taaagctgca agagaaatga
     3241 aagaacgtgt tcaaaaatta gtaggtgatc aagcagaagt tatttggatg tcaacattcc
     3301 actcaatgtg tgttcgtatt ttacgtcgtg atgcagatcg aattggtata gaacgcaatt
     3361 ttacgataat tgatcctaca gaccaaaaat ctgttattaa agacgtctta aaaaatgaaa
     3421 atattgatag taaaaagttt gaacctcgta tgtttatcgg tgcgatcagt aatttgaaaa
     3481 atgaacttaa aacacctgca gatgctcaaa aagaagccac agattatcac tcgcaaatgg
     3541 tagcaacggt ttatagtgga tatcaacgcc aattgtcacg taatgaagcg ttagattttg
     3601 atgaccttat tatgacaacg attaacttat ttgagcgtgt accagaagtt ctagaatatt
     3661 atcagaacaa attccaatat attcatgtag atgagtatca agatactaat aaagcacaat
     3721 acacattagt taaattatta gcaagtaagt ttaaaaactt atgtgttgta ggtgactcag
     3781 atcagtcaat ttatggttgg cgtggtgctg atattcaaaa tatcttatca tttgaaaaag
     3841 actatccaga agcgaataca atctttttag agcaaaatta tcgttcgacg aaaacgattt
     3901 taaatgcggc taacgaagtg attaaaaata attctgaacg taagccaaaa ggactgtgga
     3961 ctgcaaatac gaatggtgag aaaattcatt actatgaagc aatgacggaa cgtgatgaag
     4021 cggaatttgt aatacgagaa attatgaagc atcaacgtaa tggtaagaaa tatcaagata
     4081 tggcaatttt atatagaacg aatgcacaat cacgtgtact tgaggaaaca ttcatgaaat
     4141 ctaatatgcc atacacaatg gttggtggcc aaaagttcta tgaccgtaaa gaaatcaaag
     4201 atttattaag ttatttgcgt ataattgcca atagtaatga tgacattagt ttgcaacgta
     4261 ttattaatgt tccaaaaaga ggtgtaggtc cttcatctgt tgaaaaagtt caaaactatg
     4321 cacttcaaaa caatatcagt atgtttgatg cacttggaga agctgatttt atcggattgt
     4381 caaaaaaggt gacacaagag tgtcttaatt tttacgagtt aatccaaagc ttgataaaag
     4441 aacaagaatt tttagaaatt catgaaatag ttgatgaagt attacaaaag tctggctatc
     4501 gagaaatgct tgaacgtgaa aatacattag aatctcgaag tagattagaa aacatcgatg
     4561 aatttatgtc agtaccaaaa gactatgagg aaaatacccc attagaagaa cagtcattaa
     4621 ttaacttttt aacggattta tcgttagtag ctgatattga tgaggcagat actgaaaatg
     4681 gcgtaacact aatgacgatg cactcggcta agggccttga atttccaatt gtctttataa
     4741 tggggatgga agaatcttta ttcccacata ttagagcgat taagagtgaa gatgatcatg
     4801 aaatgcaaga agaacgtcgt atttgttatg tagcaattac aagggctgaa gaggtgttat
     4861 atatcactca tgcgacatca agaatgttat ttggtcgccc tcagtcaaat atgccatcca
     4921 gatttttaaa ggaaattcca gaatcactat tagaaaatca ttcaagtggc aaacgacaaa
     4981 cgatacaacc taaggcaaaa ccttttgcta aacgcggatt tagtcaacga acaacgtcaa
     5041 cgaaaaaaca agtattgtca tctgattgga atgtaggtga caaagtgatg cataaagcct
     5101 ggggagaagg catggtgagt aatgtaaacg agaaaaatgg ctcaatcgaa ctagatatta
     5161 tctttaaatc acaagggcca aaacgtttgt tagcgcaatt tgcaccaatt gaaaaaaagg
     5221 aggattaagg gatggctgat ttatcgtctc gtgtgaacga gttacatgat ttattaaatc
     5281 aatacagtta tgaatactat gtagaggata atccatctgt accagatagt gaatatgaca
     5341 aattacttca tgaactgatt aaaatagaag aggagcatcc tgagtataag actgtagatt
     5401 ctccaacagt tagagttggc ggtgaagccc aagcctcttt caataaagtc aaccatgaca
     5461 cgccaatgtt aagtttaggg aatgcattta atgaggatga tttgagaaaa ttcgaccaac
     5521 gcatacgtga acaaattggc aacgttgaat atatgtgcga attaaaaatt gatggcttag
     5581 cagtatcatt gaaatatgtt gatggatact tcgttcaagg tttaacacgt ggtgatggaa
     5641 caacaggtga agatattacc gaaaatttaa aaacaattca tgcgatacct ttgaaaatga
     5701 aagaaccatt aaatgtagaa gttcgtggtg aagcatatat gccgagacgt tcatttttac
     5761 gattaaatga agaaaaagaa aaaaatgatg agcagttatt tgcaaatcca agaaacgctg
     5821 ctgcgggatc attaagacag ttagattcta aattaacggc aaaacgaaag ctaagcgtat
     5881 ttatatatag tgtcaatgat ttcactgatt tcaatgcgcg ttcgcaaagt gaagcattag
     5941 atgagttaga taaattaggt tttacaacga ataaaaatag agcgcgtgta aataatatcg
     6001 atggtgtttt agagtatatt gaaaaatgga caagccaaag agagtcatta ccttatgata
     6061 ttgatgggat tgttattaag gttaatgatt tagatcaaca ggatgagatg ggattcacac
     6121 aaaaatctcc tagatgggcc attgcttata aatttccagc tgaggaagta gtaactaaat
     6181 tattagatat tgaattaagt attggacgaa caggtgtagt cacacctact gctattttag
     6241 aaccagtaaa agtagctggt acaactgtat caagagcatc tttgcacaat gaggatttaa
     6301 ttcatgacag agatattcga attggtgata gtgttgtagt gaaaaaagca ggtgacatca
     6361 tacctgaagt tgtacgtagt attccagaac gtagacctga ggatgctgtc acatatcata
     6421 tgccaaccca ttgtccaagt tgtggacatg aattagtacg tattgaaggc gaagtagcac
     6481 ttcgttgcat taatccaaaa tgccaagcac aacttgttga aggattgatt cactttgtat
     6541 caagacaagc catgaatatt gatggtttag gcactaaaat tattcaacag ctttatcaaa
     6601 gcgaattaat taaagatgtt gctgatattt tctatttaac agaagaagat ttattacctt
     6661 tagacagaat ggggcagaaa aaagttgata atttattagc tgccattcaa caagctaagg
     6721 acaactcttt agaaaattta ttatttggtc taggtattag gcatttaggt gttaaagcga
     6781 gccaagtgtt agcagaaaaa tatgaaacga tagatcgatt actaacggta actgaagcgg
     6841 aattagtaga aattcatgat ataggtgata aagtagcaca atctgtagtt acttatttag
     6901 aaaatgaaga tattcgtgct ttaattcaaa aattaaaaga taaacatgtt aatatgattt
     6961 ataaaggtat caaaacatca gatattgaag gacatcctga atttagtggt aaaacgatag
     7021 tactgactgg taagctacat caaatgacac gcaatgaagc atctaaatgg cttgcatcac
     7081 aaggtgctaa agttacaagt agcgttacta aaaatacaga tgtcgttatt gctggtgaag
     7141 atgcaggttc aaaattaaca aaagcacaaa gtttaggtat tgaaatttgg acagagcaac
     7201 aatttgtaga taagcaaaat gaattaaata gttagagggg tatgtcgatg aagcgtacat
     7261 tagtattatt gattacagct atctttatac tcgctgcttg tggtaaccat aaggatgacc
     7321 aggctggaaa agataatcaa aaacataaca atagttcaaa tcaagtaaaa gaaattgcaa
     7381 cggataaaaa tgtacaaggt gataactatc gtacattgtt accatttaaa gaaagccagg
     7441 caagaggact tttacaagat aacatggcaa atagttataa tggcggcgac tttgaagatg
     7501 gtttattgaa cttaagtaaa gaagtatttc caacagataa atatttgtat caagatggtc
     7561 aatttttgga caagaaaaca attaatgcct atttaaatcc taagtataca aaacgtgaaa
     7621 tcgataaaat gtctgaaaaa gataaaaaag acaagaaagc gaatgaaaat ttaggactta
     7681 atccatcaca cgaaggtgaa acagatcctg aaaagattgc agaaaaatca ccagcctatt
     7741 tatctaacat tttagagcaa gatttttatg gtggtggaga tacaaaaggt aagaatatta
     7801 aaggtatgac gattggttta gctatgaata gtgtttatta ctataaaaaa gaaaaagatg
     7861 gatcgacttt tagtaaaaaa ctagatgata gcgaagttaa aaagcaaggt aaacaaatgg
     7921 ctagtgagat attatcaagg ttacgtgaaa atgatgattt aaaagatata ccaattcatt
     7981 ttgcaattta taagcaatca agtgaagatt caatcacacc aggtgaattt atcactcaag
     8041 cgactgcaga aaagagtcaa acaaagctta atgaatggca taatatcaat gaaaaatcag
     8101 ctttattacc ttcttcaaca gcagcagatt atgatgaaaa tttaaataat aatttcaagc
     8161 aatttaatga taatttgcaa tcatattttt ctaatttcac acaagcagta gggaaagtta
     8221 aatttgttga taaaaagcca caacgattag tagtagattt accaatcgat tactatggac
     8281 aagctgaaac aattggtatt acacagtacg ttactgaaca agcgaataaa tatttcgata
     8341 aaatcgataa ctatgaaatt cgtattaaag atggtaacca accacgtgct ttaattagta
     8401 agacaaaaga tgacaaagaa ccgcaagttc atatttacag taattaattg taaattaaag
     8461 ataataaaaa ggtaccaatg ttagtcataa ttttgattaa cattagtacc tttttaattt
     8521 gttaagactt atgaattatt tttctctaac gatgtcacga actttgttta agtcagtttc
     8581 aacaaatgca ccaggttttt tagtaagttt acttacaaca tatgtaacga ttacacttac
     8641 aataaatcca ggaataattt catataagcc gaatatttcg ttgatatgtg ccaatggttt
     8701 aatccacgca atccaaacga taacgactaa ggcacctgaa accattccac ttacagcacc
     8761 ggcacgtgtc aaacctttcc agtaaagtgc aaatagcaca agtggactga acgatgcacc
     8821 aaatccggcc caagcgttac ctactaagtt tagaattgtg tcgtttggat tccatgcaat
     8881 cgcgatggca acaattgcta caactaatac agataatctt ccaatcataa caaattcttt
     8941 ttggtgcgtt ttagcttttt cttcaccacg aattaattta taaaagtctt ccgttagtga
     9001 actagatgtt acaagtaatt gtgaagaaat cgtgctcata attgcagcta gaatcgcagc
     9061 aagtaagaaa ccacctacaa gaggatggaa gagtacttga ctcatcacga tgaataatgt
     9121 ctcaggatct tctagtttaa tatgataagc aggtacgaat gcaatacctg ttaaaccaac
     9181 agccacagcg cctaataaac caacagccat ccagctaata cctaaacgtc tagctttagg
     9241 tagcatcttg tgtgatttaa tagacataaa ccttacaatg atatgaggtt gaccgaaata
     9301 acctaatccc catgaaaata gagagataat tcctataaat gataaccctt taaataaatt
     9361 taaatttgta ggtttcatag ctgctacatc atgaaacgtt ccccagccgt ttaaattcat
     9421 catagcaaca atagggacca taaccatcgc aattaacata atgacacctt ggaagaaatc
     9481 tgtaattgat acagctaaat atccaccaaa gaaagtatag aaaatgacaa tgaaagcaac
     9541 taatattaaa ccgaaatgat aatctaatcc aaaagcactt tcaaatagtt taccaccaga
     9601 tacgaaacca gaatgtgtat ataatgtaaa gaatactacg ataatcaatc cagaaataat
     9661 ctttaacaca ttatttttat cgtttaaacg atttttaaag aaatctggta atgtaattgc
     9721 atctccagct aattcggtat aaacacgaag tctaggagca acaacaaagt aatttatata
     9781 agcacctaat gttaaaccga ttgtaatcca catagttgat agaccagtgc tatagacaga
     9841 accaggtagc cccataatca tccatccact catatctgaa gctccagctg ataatgcagt
     9901 aatatacggt ccaatactac gtccacctaa catgtactcg cttaggttac cagttgcttg
     9961 cttgtaaccg taaaagccaa taacgattag tatcaagaag tagacggcaa tcataatata
    10021 agtttgccaa ttggcatcta cttgttgact taatgctgtc cccattgtaa gcatgataaa
    10081 cactcccttt atttaattta taatttagtt acttgaaaac attatacata aagataaatg
    10141 tttagaaaag taggaaaatt aaatttattc tttttgaaat acttgcatac caatatagaa
    10201 agcgttttcg tttttttgga tatgagcaaa tttttataat taaaattgca taatcataat
    10261 attgaattgg gttgtctaat attaaaagag tgttgttttc ataaaaaatg atttgataaa
    10321 cgaatgataa tgtttataca gtttatatga aattaaagta gcacctcata aatacttaga
    10381 tttttaattg gaaatttgat acaatttagt gatgaatgac ttaaaggagg cttttattaa
    10441 tgacaaaagt aacacgtgaa gaagttgagc atatcgcgaa tcttgcaaga cttcaaattt
    10501 ctcctgaaga aacggaagaa atggccaaca cattagaaag cattttagat tttgcaaaac
    10561 aaaatgatag cgctgataca gaaggcgttg aacctacata tcacgtttta gatttacaaa
    10621 acgttttacg tgaagataaa gcaattaaag gtattccaca agaattagct ttgaaaaatg
    10681 ccaaagaaac agaagatgga caatttaaag tgcctacaat catgaatgag gaggacgcgt
    10741 aagatgagca ttcgctacga atcggttgag aatttattaa ctttaataaa agacaaaaaa
    10801 atcaaaccat ctgatgttgt taaagatata tatgatgcaa ttgaagagac tgatccaaca
    10861 attaagtctt ttctagcgct ggataaagaa aatgcaatca aaaaagcgca agaattggat
    10921 gaattacaag caaaagatca aatggatggc aaattatttg gtattccaat gggtataaaa
    10981 gataacatta ttacaaacgg attagaaaca acatgtgcaa gtaaaatgtt agaaggtttt
    11041 gtgccaattt acgaatctac tgtaatggaa aaactacata atgaaaatgc cgttttaatc
    11101 ggtaaattaa atatggatga gtttgcaatg ggtggttcaa cagaaacatc ttatttcaaa
    11161 aaaacagtta acccatttga ccataaagca gtgccaggtg gttcatcagg tggatctgca
    11221 gcagcagttg cagctggctt agtaccattt agcttaggtt cagacacagg tggttcaatt
    11281 agacaaccgg ctgcatattg tggcgttgtc ggtatgaaac caacatacgg tcgtgtatct
    11341 cgatttggat tagttgcttt tgcatcttca ttagaccaaa ttggtccatt gactcgaaat
    11401 gtaaaagata atgcaatcgt attagaagct atttctggtg cagatgttaa tgactctaca
    11461 agtgcaccag ttgatgatgt agactttaca tctgaaattg gtaaagatat taaaggatta
    11521 aaagttgcat tacctaaaga atacttaggt gaaggtgtag ctgatgacgt aaaagaagca
    11581 gttcaaaacg ctgtagaaac tttaaaatct ttaggtgctg tcgttgagga agtatcattg
    11641 ccaaatacta aatttggtat tccatcatat tacgtgattg catcatcaga agcttcgtca
    11701 aacctttctc gttttgacgg aattcgttat ggttatcatt ctaaagaagc tcattcatta
    11761 gaagaattat ataaaatgtc aagatctgaa ggtttcggta aagaagtaaa acgtcgtatt
    11821 ttcttaggta catttgcatt aagttcaggt tactatgatg cttactataa aaaatctcaa
    11881 aaagttagaa cattgattaa aaatgacttt gataaagtat tcgaaaatta tgatgtagta
    11941 gttggtccaa cagcgcctac aactgcgttt aatttaggtg aagaaattga tgatccatta
    12001 acaatgtatg ccaatgattt attaacaaca ccagtaaact tagctggatt acctggtatt
    12061 tctgttcctt gtggacaatc aaatggccga ccaatcggtt tacagttcat tggtaaacca
    12121 ttcgatgaaa aaacgttata tcgtgtcgct tatcaatatg aaacacaata caatttacat
    12181 gacgtttatg aaaaattata aggagtggaa atcatgcatt ttgaaacagt tataggactt
    12241 gaagttcacg tagagttaaa aacggactca aaaatgtttt ctccatcacc agcgcatttt
    12301 ggagcagaac ctaactcaaa tacaaatgtt atcgacttag catatccagg tgtcttacca
    12361 gttgttaata agcgtgcagt agactgggca atgcgtgctg caatggcact aaatatggaa
    12421 atcgcaacag aatctaagtt tgaccgtaag aactatttct atccagataa tccaaaagca
    12481 tatcaaattt ctcaatttga tcaaccaatt ggtgaaaatg gatatatcga tatcgaagtc
    12541 gacggtgaaa caaaacgaat cggtattact cgtcttcaca tggaagaaga tgctggtaag
    12601 tcaacacata aaggtgagta ttcattagtt gacttgaacc gtcaaggtac accgctaatt
    12661 gaaatcgtat ctgaaccaga tattcgttca cctaaagaag catatgcata tttagaaaaa
    12721 ttgcgttcaa ttattcaata cactggtgta tcagacgtta agatggaaga gggatcttta
    12781 cgttgtgatg ctaacatctc tttacgtcca tatggtcaag aaaaatttgg tactaaagcc
    12841 gaattgaaaa acttaaactc atttaactat gtacgtaaag gtttagaata tgaagaaaaa
    12901 cgccaagaag aagaattgtt aaatggtgga gaaatcggac aagaaacacg tcgatttgat
    12961 gaatctacag gtaaaacaat tttaatgcgt gttaaagaag gttctgatga ttaccgttac
    13021 ttcccagagc ctgacattgt acctttatat attgatgatg cttggaaaga gcgtgttcgt
    13081 cagacaattc ctgaattacc agatgaacgt aaagctaagt atgtaaatga attaggttta
    13141 cctgcatacg atgcacacgt attaacattg actaaagaaa tgtcagattt ctttgaatca
    13201 acaattgaac acggtgcaga tgttaaatta acatctaact ggttaatggg tggcgtaaac
    13261 gaatatttaa ataaaaatca agtagaatta ttagatacta aattaacacc agaaaattta
    13321 gcaggtatga ttaaacttat cgaagacgga acaatgagca gtaaaattgc gaagaaagtc
    13381 ttcccagagt tagcagctaa aggtggtaat gctaaacaga ttatggaaga taatggctta
    13441 gttcaaattt ctgatgaagc aacacttcta aaatttgtaa atgaagcatt agacaataac
    13501 gaacaatcag ttgaagatta caaaaatggt aaaggcaaag ctatgggctt cttagttggt
    13561 caaattatga aagcgtctaa aggtcaagct aatccacaat tagtaaatca actattaaaa
    13621 caagaattag ataaaagata atttaaatca tcaaactatg aagatttaaa aaataaaccc
    13681 t
//
LOCUS       DAXWFK010000060        13616 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.060, whole genome shotgun sequence.
ACCESSION   DAXWFK010000060 DAXWFK010000000
VERSION     DAXWFK010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13616
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.060"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            122..1246
                     /locus_tag="ACGEXS_001358"
     CDS             122..1246
                     /locus_tag="ACGEXS_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFF8012376.1"
                     /translation="MHKKYLLLFFLLLSVALLYSCSKQNEKAQNKSVNPNEYAKHKSK
                     MDEHWETYHGQVYHVFYHPLITDPKVAFTGDTHQAKGNNDWMITVSEFKKSLNELYKN
                     NYIIVNPHDVYDLSSHHVKAKKIKLPKGKKPLIISIDDMNYYSYMRHHGYADRLVLDK
                     NKHVVSETTDKKGHATYSDDNDIVPILNKFVKEHPDFSLNGEKGVVALTGYEGVLGYR
                     TNELTSKDYTKNKKAAEEVVRAMKRDGWSFASHSYGHINFEKTSLEGIKRDTKRWKDE
                     VEPIVGKTDMFVFPHGAQDRHTQAYDYLVDEAEFKFIAGVGPNNFTDISATNVYQDRV
                     AIDGLNLFEFKYKLKPFFNPENVYSKQDRRYFKGNRDYEE"
     gene            1551..2369
                     /locus_tag="ACGEXS_001359"
     CDS             1551..2369
                     /locus_tag="ACGEXS_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HFF8012377.1"
                     /translation="MNNSEQLIALKESETAFLKYFNKADYELVDFSVVEKLDWKQLNH
                     EDLQQMGERNFWQHEHQIYALRNDFTDQLLRYYSMYPTAATKVAYTGLIIRNNEAAVQ
                     VGLENYAPSLANVQQSLKLFIQFIQQQLRDNVHFVVLGHYQLLDALLDKSLQTPDILS
                     MIEERNLSGLVTYLSTEHPIVQILKENTQQQLNVLEHYIPNDHPALVELKIWERWLHK
                     QGYKDIHLDITAQPPRSYYTGLFIQCHFAENESRVLTGGYYKGSIEGFGLGLTL"
     gene            2387..3001
                     /gene="hisG"
                     /locus_tag="ACGEXS_001360"
     CDS             2387..3001
                     /gene="hisG"
                     /locus_tag="ACGEXS_001360"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFF8012378.1"
                     /translation="MLRIAIAKGRLMDSLINYLDVIEYTTLSETLKNRERQLLLSVDN
                     IECILVKGSDVPIYVEQGMADIGIVGSDILDERQYNVNNLLNMPFGACHFAVAAKPET
                     TNYRKIATSYVHTAETYFKSKGIDVELIKLNGSVELACVVDMVDGIVDIVQTGTTLKA
                     NGLVEKQHISDINARLITNKAAYFKKSQLIEQFIRSLEVSIANA"
     gene            2994..4244
                     /gene="hisD"
                     /locus_tag="ACGEXS_001361"
     CDS             2994..4244
                     /gene="hisD"
                     /locus_tag="ACGEXS_001361"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFF8012379.1"
                     /translation="MLNAQQFLNQFSLEAPLDESLYPIIRDICQEVKVHGDKALKMYN
                     LTFDHTKTDHLEISHEQIKAAFDTLDEKTKQALQQSYERIKAYQESIKQTNQQLEESV
                     ECYEIYHPLESVGIYVPGGKASYPSTVLMTATLAQVAGVENIVVVTPPQPNGVSQEVL
                     AACYITQVDQVFQVGGAQSIAALTYGTETIPKVDKIVGPGNQFVAYAKKYLFGQVGID
                     QIAGPTEIALIIDETADLDAIVYDVFAQAEHDELARTYAISEDAQVLKDLESRIAKAL
                     PNVDRYDIVSKSIANQHYLIHASNFDEACHVMNTIAPEHASIQTVNPQPYIEKVKYVG
                     ALFIGHYSPEVIGDYVAGPSHVLPTNRTARFTNGLSVNDFLTRNTVIHLSKDTFEQIA
                     DSAQHIAHVEALYNHQQSILIRQS"
     gene            4260..5273
                     /locus_tag="ACGEXS_001362"
     CDS             4260..5273
                     /locus_tag="ACGEXS_001362"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFF8012380.1"
                     /translation="MIYIDKNESPVTPLDEKTMTSIISATPYNLYPDAAYEQFKEAYA
                     KFYGLSPEQIIAGNGSDELIQKLMLIMPEGPALTLNPDFFMYQAYAAQVNREIAFVDA
                     GSDLTFDLETILTKIDEVQPSFFIMSNPHNPSGKQFDTAFLTAIADKMKALNGYFVID
                     EAYLDYGTAYDVELAPHILRMRTLSKAFGIAGLRLGVLISTAGTIKHIQKIEHPYPLN
                     VFTLNIATYIFRHREETRQFLTMQRQLAEQLKQIFDTHVADKMSVFPSNANFVLTKGS
                     AAQQLGQYVYEQGFKPRFYDEPVMKGYVRYSIATASQLKQLEEIVKEWSAKYDLSKTT
                     KHS"
     gene            5242..5820
                     /gene="hisB"
                     /locus_tag="ACGEXS_001363"
     CDS             5242..5820
                     /gene="hisB"
                     /locus_tag="ACGEXS_001363"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HFF8012381.1"
                     /translation="MIYQKQRNTAETQLNISISDDQSPSHINTGVGFLNHMLTLFTFH
                     SGLSLNIEAQGDIDVDDHHVTEDIGIVIGQLLLEMIKDKKHFVRYGMMYIPMDETLAR
                     VVVDISGRPYLSFNASLSKEKVGTFDTELVEEFFRAVVINARLTTHIDLIRGGNTHHE
                     IEAIFKAFSRALGIALTATDDQRVPSSKGVIE"
     gene            5817..6395
                     /gene="hisH"
                     /locus_tag="ACGEXS_001364"
     CDS             5817..6395
                     /gene="hisH"
                     /locus_tag="ACGEXS_001364"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFF8012382.1"
                     /translation="MIVIVDYGLGNISNVKRAIEHLGYEVVVSNTSKIIDQAETIILP
                     GVGHFKDAMSEIKRLNLNAILAKNTDKKMIGICLGMQLMYEHSDEGDASGLGFIPGNI
                     SRIQTEYPVPHLGWNNLVSKHPMLNQDVYFVHSYQAPMSENVIAYAQYGADIPAIVQF
                     NNYIGIQFHPEKSGTYGLQILRQAIQGGFIND"
     gene            6388..7092
                     /gene="hisA"
                     /locus_tag="ACGEXS_001365"
     CDS             6388..7092
                     /gene="hisA"
                     /locus_tag="ACGEXS_001365"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFF8012383.1"
                     /translation="MIELWPAIDLIGSTSVRLTEGKYDSEEKMSRSAEESIAYYSQFE
                     CVNRIHIVDLIGAKAQHAREFDYIKSLRRLTTKDIEVGGGIRTKSQIMDYFAAGINYC
                     IVGTKGIQDTDWLKEMAHTFPGRIYLSVDAYGEDIKVNGWEEDTELNLFSFVRRLSDI
                     PLGGIIYTDIAKDGKMSGPNFELTGQLVKATTIPVIASGGIRHQQDIQRLASLNVHAA
                     IIGKAAHQASFWEGLK"
     gene            7089..8476
                     /gene="hisF"
                     /locus_tag="ACGEXS_001366"
                     /pseudo
     CDS             7089..8476
                     /gene="hisF"
                     /locus_tag="ACGEXS_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501457.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
     gene            8669..8848
                     /locus_tag="ACGEXS_001367"
     CDS             8669..8848
                     /locus_tag="ACGEXS_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012384.1"
                     /translation="MKSKLSNSGIDVTVKDVEKYMNRYNEVMKGKNGEKAKELCLSLL
                     PINIIVVFTFFVFIL"
     gene            9569..11610
                     /gene="lip1"
                     /locus_tag="ACGEXS_001368"
                     /pseudo
     CDS             9569..11610
                     /gene="lip1"
                     /locus_tag="ACGEXS_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501455.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing triacylglycerol lipase
                     Lip1"
     gene            complement(12057..13109)
                     /gene="icaC"
                     /locus_tag="ACGEXS_001369"
     CDS             complement(12057..13109)
                     /gene="icaC"
                     /locus_tag="ACGEXS_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide intercellular adhesin
                     biosynthesis/export protein IcaC"
                     /protein_id="HFF8012385.1"
                     /translation="MKKIRLELVYLRAIICAIIIITHLLTQITLKHENMEGGSLVLQF
                     YIRNIVIFGTPCFIILSQLLTTLNYQKVTYRYLTTRVKYILIPYILMGLFYSYSESLL
                     TDSSFNKQFIENVLLGQWYGYFIVVIMQFFILSYIIFKINYNLFNSKILLLLSFILQQ
                     SFLYYFTNNTAFHDTVLHYYPLSENTIIFGWIFYFFLGAYMGYNYERVLNFLERYLVI
                     MIVLAVATYFVFIALANGDYWNVTSFSYSLTPYNSIMFIVILGICTHFKTMLFNTIQM
                     ISAFSFFIYLLHPIILDSLFAYTNIFEDNTMVFLAISLLFILGLCIGVGMILREFYIF
                     RFIIGKQPYKLNINAY"
     gene            complement(13096..>13616)
                     /gene="icaB"
                     /locus_tag="ACGEXS_001370"
     CDS             complement(13096..>13616)
                     /gene="icaB"
                     /locus_tag="ACGEXS_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="intercellular adhesin biosynthesis
                     polysaccharide N-deacetylase"
                     /protein_id="HFF8012386.1"
                     /translation="NFDDMDETIYENAYPILKKYKIPATGFIITGHVGEENFHNLDMI
                     SKKELKEMYKTGLWEFETHTHDLHNLSKNNKSKLMKASEATIIKDLNKSEKYLTKNFK
                     KSQKTIAYPYGLMNDDKLPVIKKAGLKYGFSLEEKAVTPNSNDYYIPRILISDDAFEH
                     LIKRWDGFHEKD"
BASE COUNT         5029 a         2005 c         2561 g         4021 t
ORIGIN      
        1 ataaaatact atgacattat ctaaaaaata aaattaaaat cccaacagtt tcttaaaaag
       61 ttatgagcca cataataaaa atgttataat tgtgcataat aaaacatagg gagaatgttt
      121 aatgcataaa aaatatttat tgctattttt tctgttatta tcagttgcat tgttatatag
      181 ctgttccaag caaaatgaga aagctcaaaa taaatctgtt aatccaaatg agtatgcaaa
      241 gcataaatcg aaaatggatg aacattggga aacatatcac ggccaagttt atcatgtgtt
      301 ttatcaccca ttaattacag acccaaaagt tgcgtttaca ggagatacac accaagctaa
      361 gggcaacaat gattggatga ttactgtaag tgaatttaaa aaatcattga acgaattata
      421 taaaaataat tacatcatcg ttaatccaca tgatgtatac gacttatcat ctcatcatgt
      481 aaaagccaaa aaaataaaat tgccgaaagg taaaaagcca ctaatcatct caattgatga
      541 tatgaattac tatagttaca tgagacacca tggttatgct gaccgtttag tactcgataa
      601 aaataaacat gttgtttctg aaactacaga caaaaaaggc catgctactt attctgatga
      661 caatgatatc gtgccaattt taaataaatt tgtaaaagag catccagatt tttctttaaa
      721 tggtgaaaaa ggtgttgttg ctttaaccgg ttatgagggc gtattaggtt atcgcacgaa
      781 tgaattaacg agtaaagatt atacaaagaa taaaaaggca gctgaggaag ttgttcgcgc
      841 aatgaaacgg gatggatggt catttgcgag tcattcatat ggtcacatta attttgaaaa
      901 aacatcttta gaaggtatta aacgtgatac taaaagatgg aaagatgaag ttgaaccaat
      961 tgtaggtaaa acagatatgt ttgtatttcc acatggcgca caggatagac atacacaagc
     1021 gtatgattat ttggtagatg aagcagagtt taagtttatt gcaggtgtcg gtccaaacaa
     1081 ttttacagat atatcagcaa cgaacgttta ccaagatagg gttgcgattg atggtctaaa
     1141 cttatttgaa tttaaatata aactcaaacc gttttttaat cctgaaaatg tatatagtaa
     1201 acaagataga cgttacttta aagggaatcg ggattatgaa gaatagttaa atttaataaa
     1261 aagatagaaa gaaaataact ttacataata aggcaaggac aatatgtgca agaagtacaa
     1321 cttcaatata aaaatcttgg ttaagcattt ttataggcac aaatgtcttg ccttttttat
     1381 tttaaaaaat tttaaaatca atacaccgaa ttttctgaaa aaggttgtaa tgtattgcta
     1441 aaaatgataa gttattaatc atattacact ttagcgtggt agagaagtaa agcaaacaac
     1501 gaaaatacca agtgatttta aatacagtat tgaaagtaaa gggtggtcga gtgaataatt
     1561 cagaacaatt aattgcgtta aaagaatcgg aaacagcatt tttaaaatat tttaataaag
     1621 cggattatga gttagttgat tttagtgtgg ttgaaaagtt ggactggaaa caattgaacc
     1681 atgaggattt gcagcagatg ggtgagcgaa atttttggca acatgagcat caaatttatg
     1741 cacttcgaaa tgattttaca gatcaattat tgcgttatta ctcaatgtat ccaactgcag
     1801 caacaaaagt ggcatacaca ggtttaatca ttcggaataa tgaagcggct gtacaagttg
     1861 gtttggaaaa ctatgcacca tcattagcga atgtgcaaca gagcttaaag ctctttattc
     1921 agtttattca acagcagttg agagacaacg tgcattttgt tgtattggga cattatcaat
     1981 tgcttgatgc cttactcgat aaatcgttac aaacaccaga catactatca atgattgagg
     2041 aacgtaattt atcgggactg gttacatatt tgtcgactga acatccaatc gtgcaaatct
     2101 tgaaagagaa tacgcaacaa caattaaatg tattggaaca ctatattcca aatgaccatc
     2161 cagcactcgt tgaattaaaa atatgggaac gttggttaca taaacaaggt tacaaagaca
     2221 tccatttaga tattactgcg cagccaccta gatcctatta cacaggttta tttattcaat
     2281 gtcattttgc tgaaaatgaa tctcgagttt taacaggtgg ttattacaaa ggaagcatcg
     2341 aagggtttgg attaggatta acactttaag taagggagta tgcacaatgt taagaatcgc
     2401 catagccaaa ggacgtctaa tggatagttt aattaactat ttagatgtaa ttgaatatac
     2461 gacattatca gaaacattaa aaaatagaga acgccaatta ttattaagtg tagataatat
     2521 tgaatgcatt ttagtaaaag gaagtgacgt gccaatctat gtggaacaag gaatggcaga
     2581 cataggcatt gttggtagcg acatattaga tgagcgccaa tataatgtta ataatttgtt
     2641 gaatatgcct tttggagcat gtcattttgc ggttgcagcg aaacctgaaa cgaccaatta
     2701 tcgtaaaatc gcaacgagtt atgttcatac tgctgaaaca tattttaaat caaaaggtat
     2761 tgatgtcgaa ttgattaaat tgaatggctc tgttgaattg gcctgtgttg tagatatggt
     2821 agacggaatt gtcgacatcg ttcaaacagg tactacgcta aaagcgaacg gactggttga
     2881 aaagcaacat attagtgata tcaatgcaag attaataact aataaagcag cttattttaa
     2941 aaaatcacaa ttaatagagc aatttattcg ctctttggag gtgtctattg ccaatgctta
     3001 atgcacaaca atttttaaat caattttcat tagaagcacc attagatgag tcattgtatc
     3061 caattattcg cgatatttgt caggaagtta aagttcatgg agataaggct ttgaaaatgt
     3121 ataatctaac attcgatcat acgaaaacag atcatttaga aattagtcat gaacaaatta
     3181 aagcagcatt tgacacatta gatgaaaaaa caaaacaagc attacaacaa agttatgaaa
     3241 gaattaaagc atatcaagaa agtattaaac agacgaatca acagttagaa gaatcagtgg
     3301 agtgttatga aatataccat ccactagaaa gtgtcggtat ttatgtgcct ggtggtaaag
     3361 caagttatcc ttcaacggta ttaatgacag cgacgttagc acaagtagca ggtgtggaaa
     3421 atattgtcgt tgtgacacca cctcaaccta atggtgtgtc tcaagaagta ttagcagcat
     3481 gctatattac gcaagttgat caagtatttc aagttggtgg tgctcaaagt attgctgcgt
     3541 taacttatgg cacagaaacg atacctaaag ttgataagat tgtaggtcca ggcaatcaat
     3601 ttgttgcata tgccaagaaa tatttatttg gacaagtagg tattgaccaa atagctggac
     3661 caacagaaat agcacttatt attgatgaaa cagcagattt agatgccatc gtatatgatg
     3721 tttttgcaca agcagaacat gatgaattag cacgtacata tgccattagt gaagatgcgc
     3781 aagtccttaa agatttagaa tcacgtattg ctaaagcatt gcctaatgtg gacagatacg
     3841 acattgtttc taaaagtatc gctaatcaac actaccttat ccatgctagt aattttgatg
     3901 aagcatgcca tgtcatgaat acaatcgcgc ctgaacatgc gtcgattcaa acagtaaatc
     3961 ctcaaccata tattgaaaaa gtgaaatatg tgggtgcatt gtttattgga cattattcgc
     4021 cagaggtcat aggagattac gttgcaggtc caagtcatgt attacctaca aatagaacag
     4081 ctagatttac caatgggtta tcggtcaatg atttcttaac acggaacacg gtcatccatt
     4141 tatcaaaaga tacgtttgaa caaattgctg attcagcaca acatattgct catgttgaag
     4201 cattatacaa tcaccagcag tctattttaa tacgtcagtc ttaggggagt gtaattgaaa
     4261 tgatttatat tgataaaaat gaaagtccag ttacgccgtt ggatgaaaaa acaatgacgt
     4321 ctattattag tgcaacgcca tataatttat atcctgatgc agcatatgaa caattcaagg
     4381 aagcttatgc taagttttac ggattatcgc ctgaacaaat tattgcagga aatggatctg
     4441 atgaattgat tcaaaagtta atgctgatca tgccagaagg tccggcatta acgctaaatc
     4501 ctgatttttt tatgtatcaa gcatatgcgg cacaagtaaa tcgtgaaatt gcatttgtag
     4561 atgcaggatc agatttaacg tttgatttgg aaaccatttt aacgaaaatc gatgaagtac
     4621 aaccatcatt ttttattatg agtaatccac ataacccttc aggcaagcaa tttgatacgg
     4681 catttttaac agctattgca gataagatga aagcattaaa cggatacttt gtcattgatg
     4741 aagcatattt agattatggt acggcatatg acgtggaact ggcaccacac atcttaagaa
     4801 tgcgtacatt atcaaaggcg tttggaattg ccggcttaag attaggtgtc ttaattagta
     4861 ctgctggaac gataaagcat attcaaaaaa tagaacatcc atatccatta aatgtattta
     4921 cgctaaatat tgcgacttat atttttagac atagagaaga gacaagacaa tttttaacga
     4981 tgcaacgaca gttagctgag cagttaaaac aaatatttga tacacatgtt gcagataaaa
     5041 tgtcagtgtt cccatcaaat gctaattttg tacttactaa aggctcagca gcgcaacaat
     5101 taggacaata cgtatatgaa caaggattta aacctcgctt ttatgatgag ccggtgatga
     5161 agggctatgt aagatactca attgcaacag catcacagtt aaagcaatta gaagaaattg
     5221 ttaaagaatg gagtgcaaaa tatgatttat caaaaacaac gaaacacagc tgaaacgcaa
     5281 ctaaatattt caatatctga tgatcagtca ccatcgcata ttaatacagg tgtgggcttt
     5341 ttaaatcata tgttaacctt gtttacattt catagcggtc tgtcattaaa cattgaggca
     5401 caaggtgata ttgacgtaga tgatcaccac gtaactgaag atatcggcat tgtcattggc
     5461 caattgttac ttgaaatgat taaagataaa aagcatttcg ttcgttatgg aatgatgtac
     5521 attccaatgg atgaaacatt agcacgtgtc gttgtggata taagtgggcg cccataccta
     5581 tcattcaatg catcattaag taaagaaaaa gttggtacgt ttgatacgga gttagtagaa
     5641 gaatttttta gagcggtcgt aatcaatgca agattaacaa cgcatattga tttaattcgt
     5701 ggaggtaata cacaccatga aattgaagct atattcaaag cgttttcccg tgcattaggc
     5761 atagcgctaa ctgcaactga tgatcagcgt gtgccgtcat cgaaaggtgt gattgaatga
     5821 ttgtcatcgt tgattatgga ttagggaata ttagtaatgt aaaacgcgct attgaacatt
     5881 tagggtatga ggtggttgtc tcaaatacct caaaaataat cgatcaagca gaaacaatca
     5941 tattgcccgg tgtcggccat tttaaagatg cgatgtcaga gataaaacga ttaaatctca
     6001 atgcaatatt ggctaagaat actgataaga agatgattgg tatttgttta ggcatgcaat
     6061 taatgtatga gcatagtgat gaaggcgatg catctggatt agggtttatc ccaggaaata
     6121 tttcgcgtat ccaaacagaa tacccagtgc cacacttagg ctggaataat ttagtgagta
     6181 agcaccctat gttaaatcaa gatgtttatt tcgtacattc ttatcaagcg ccgatgtcag
     6241 aaaatgtaat tgcatatgcg cagtatgggg ctgatattcc ggcaattgtt caatttaaca
     6301 attatattgg tattcaattc catcctgaaa aaagcggtac atatgggtta caaattttgc
     6361 gtcaggcaat acaaggggga tttataaatg attgaattat ggccagcgat tgatttgatt
     6421 gggtcaacaa gtgtgaggtt aacagagggt aaatatgata gtgaagaaaa aatgtcacgc
     6481 tcggctgaag aaagtattgc ttactatagt caatttgaat gtgtgaatcg tattcatatc
     6541 gtcgacttga taggtgctaa ggcacagcat gcccgagagt ttgattatat taagtcattg
     6601 aggagattaa caaccaaaga tattgaagta ggtggtggca ttcgtacgaa gtcacaaatc
     6661 atggactact ttgccgcagg gattaattat tgcatagttg gaacgaaagg tattcaagat
     6721 actgattggc ttaaagagat ggcacataca tttccaggtc gcatttattt atctgttgat
     6781 gcctatggag aagatattaa agtgaacgga tgggaagagg acacagagtt aaatttattt
     6841 agttttgtga gacggttatc ggatatacct cttggcggca ttatatatac tgatattgct
     6901 aaagatggca aaatgtccgg acctaacttt gaattaactg gtcaattagt aaaggcaacg
     6961 acgattcctg tcattgcttc cggtggtatt agacatcagc aagatattca acgattagcg
     7021 tcgctaaatg ttcacgctgc tattatagga aaggctgcac atcaagcatc tttttgggag
     7081 gggctaaaat gattaaaaaa cgtatcattc catgtttaga tgtcaaagat ggtcgtgtcg
     7141 ttaaagggat tcaatttaaa ggattaaggg atattgggaa tcctgttgat ttagcaatgt
     7201 attacaatga agcgggtgct gatgaattag tatttttaga catctctaag acggaagagg
     7261 gtcatagctt aatgctagaa gtgattgaac agacagcgtc acgcttgttt atccctctta
     7321 ctgtaggggg tgggattcaa agtctcgatg atattaccca attgctaaat catggtgcag
     7381 ataaagtatc attaaattca agtgctttaa aaaatccaca gctcattaaa caagcgagtg
     7441 ataaattcgg tagacaatgc atctgcatag caattgatag ctattatgat cctgaaagaa
     7501 aagcacatta ttgttgtacg actggtggta aaaaaatgac aaatattaaa gtatatgact
     7561 gggtacagca agtagaacag ttaggtgcag gtgagctcct cgttacaagt atgggacatg
     7621 atggtatgaa acaaggcttt gatattgaac acctagcaaa tattaagtct cttgtaaata
     7681 ttccaatcat tgcttctggt ggtggtggca atgcacaaca ctttgtagaa ttatttgatc
     7741 agacggatgt ttctgcaggt ttagctgcaa gtatattaca tgatcgagaa acgacggttc
     7801 aatctattaa agaagtgata cggcaagggg gtatagcagt aagatgacca aatataaaat
     7861 tgattttagc aaaggtttag tgccagcaat tttacaagat aatcaaacaa aacaagtatt
     7921 gatgttgggt tatatgaacc aagaagcttt tgataaaacg atagaagatg gtgtggtatg
     7981 tttctattcg cggtcgaaac aacggctatg gacaaaaggt gaaacatctg gtcatacgca
     8041 acgtgttaaa gatattcatg tagattgcga caatgacact attttaattg atgtcatacc
     8101 aaatggacca acatgtcata caggcagtca aagttgtttc aacacagaag ttccattttc
     8161 agtgcaaaca ttagcgcaga cagttcaaga tagtgcccaa tccaataatg aaaagtcata
     8221 tacaaaatat ttattaacag aaggtataga aaagattaca aaaaaatacg gtgaagaagc
     8281 ttttgaagtc gtaattgaag caattaaagg tgacaaaaaa gcatttgtaa gtgaagtagc
     8341 agatgaactt tatcatttat ttgtcttgat gcatgcgctt ggcgtcgatt tttcagaaat
     8401 tgaggcggaa ttagcgcgta gacatcataa gcgcaataac tttaaaggtg aacgacaaaa
     8461 tatcgaacag tggtaaagca agtatggact aagatataag gaaaaggatc atggcttata
     8521 cacttacaaa tattgtggaa aacgtgacat tttcaagttt aaaatacgac accaacatat
     8581 tttaactatg aatgctgtga tggtactaaa gttgcgaact cgttatagat aagtagtgga
     8641 taatcacaat acgaaatcaa aaataattat aaaaagtaaa ttgagcaact caggaataga
     8701 tgtcactgtt aaagatgtcg aaaagtatat gaatcgatat aatgaagtta tgaagggaaa
     8761 aaatggcgaa aaagctaaag agttatgttt gtcgttacta cctattaata tcatagttgt
     8821 ctttacattc tttgtattta tactataaat acaaatatat ctagcctgaa atagaaatgt
     8881 catagcctat ttaaaagaca atctccatta gaactaagat atgcatcccg aaagttagac
     8941 taaaaaacta actttatggg atgtattttt atgctaatca tcataaattc gagattaagt
     9001 taaaggtagt tcaagagtaa ttaaacaata aattaaaaat agtaggatac ttactttgag
     9061 ggaagaaaat taactgtata tatttagttt aggaacaagt attacggttt atcctgatac
     9121 aattattgtg gatgggatga tatttttagg tttaaaatac gacaccagca aacataataa
     9181 ctgtaatagc tcataaatct ccccatatag ctaatctaaa aaaataatac atcattggaa
     9241 ttaagcccca agcatgtaaa tattaaaaat caaaaaagat atatgtaaaa aagttacaat
     9301 ttgcataatt aaattgtgtc taattattga ctaattaaat tttgccaaat ataatattaa
     9361 ttaataattt gaaatgatta gcgtatacac tttaaattct ctttggagaa tatatttttt
     9421 aaatacaaat gtaaacgctt tctcgtcaaa tcaaacaata gaaaggatgg tcattatgag
     9481 tgcttggtta agtaaattat ttgagtttat tcctcgaata attatcaatt tgtttatcta
     9541 aaataaaaaa atagaggtgc tgacaatgat gaaaagtcaa aataagtata gtattcgtaa
     9601 atttagtgta ggtgcatctt ccattttaat agctacatta ctatttttaa gtggtggaca
     9661 agcacaagca gctgagaagc aagtgaatat gggaaattca caggaggata cagttacagc
     9721 acaatctatt ggggatcaac aaactaggga aaatgctaat tatcaacgtg aaaacggtgt
     9781 tgacgaacag caacatactg aaaatttaac taagaacttg cataatgata aaacaatatc
     9841 agaagaaaat catcgtaaaa cagatgattt gaataaagat caactaaagg atgataaaaa
     9901 atcatcgctt aataataaaa atattcaacg tgatacaaca aaaaataaca atgctaatcc
     9961 tagcgatgta aatcaagggt tagaacaggc tattaatgat ggtaaacaaa gtaaagtggc
    10021 gtcacagcaa cagtcaaaag aggcagataa tagtcaagat tcaaacgcta ataacaatct
    10081 accttcacaa agtcgaataa aggaagcacc atcattaaat aagttagatc aaacaaggca
    10141 acgagaaatt gttaatgaga cagaaataga gaaagtacaa ccacaacaaa ataatcaagc
    10201 gaatgataaa attactaact acaattttaa caatgaacaa gaagtgaaac ctcaaaaaga
    10261 cgaaaaaaca ctatcagttt cagatttaaa aaacaatcaa aaatcaccag tagaaccaac
    10321 aaaggacaat gacaagaaaa atggattaaa tttattaaaa agtagtgcag tagcaacgtt
    10381 accaaacaaa gggacaaagg aacttactgc aaaagcgaaa gatgatcaaa cgaataaagt
    10441 tgccaaacaa gggcagtata aaaatcagga tcctatcgtt ttagtgcatg gtttcaatgg
    10501 gtttacagat gatattaatc cttcagtgtt agctcattat tggggcggta ataaaatgaa
    10561 cattcgccaa gatttagaag aaaatggtta caaagcttat gaagcaagta taagtgcttt
    10621 tggaagtaac tatgaccgcg cagttgaact ttattattat atcaaaggcg gtcgtgtaga
    10681 ttatggtgca gcacatgcag caaaatatgg acatgaacgt tatggaaaaa catacgaagg
    10741 aatttacaaa gactggaaac caggacagaa ggtacaccta gttggacata gtatgggcgg
    10801 tcaaacgata cgtcaactag aagaattact gcgtaatggt aatcgtgaag aaatagagta
    10861 tcaaaagaaa catggtggcg aaatttctcc actattcaaa ggtaatcatg acaatatgat
    10921 ttcatcaatt actactttag gaacaccaca taatggtaca cacgcatcag atttagctgg
    10981 taatgaagct ttagtgagac aaatcgtatt tgatatcggt aaaatgtttg gtaataaaaa
    11041 ttcaagagta gacttcgggt tggctcaatg gggtctaaaa cagaagccaa atgaatcata
    11101 tattgattat gtcaaacgcg ttaaacaatc taatttatga aatcaaaaga taatggattt
    11161 tacgatctga cgcgtgaggg tgcaacagat ttaaatcgta aaacgtcgtt gaaccctaac
    11221 attgtgtata aaacatacac tggtgaagca acgcacaaag cattaaatag cgatagacaa
    11281 aaagcagact taaatatgtt tttcccattt gtgattactg gtaacttaat cggtaaagct
    11341 actgaaaaag aatggcgaga aaacgatggt ttagtatccg ttatttcttc tcaacatcca
    11401 tttaatcaag cttatacaaa agcgacagat aaaattcaaa aaggcatttg gcaagtgacg
    11461 cctacaaaac atgattggga tcatgttgac tttgtaggac aagacagttc tgatacagtg
    11521 cgcacaagag aagaattaca agatttttgg catcatttag cagacgattt agtgaaaact
    11581 gaaaagctga ctgatactaa gcaagcataa tttataaagt aaagggagga attaataatg
    11641 actgcagact tctttcaatt aatcggatca ttatttagaa ttctaaaaga attattcaag
    11701 taaaacattg gcgaggcccc aacataaaga atttcgaaaa gaaattctac aaacaatgca
    11761 agttggcggg gccccaacaa agaagctggc ggaaagtcag cttacaataa tgtgcaagtt
    11821 ggcggggccc caacatagaa gctggcggaa agtcaggtta caataatgtg caagttgggg
    11881 tgggacgacg aaataaattt tgcgaaaata tcatttctgt cccactccca ttggcattta
    11941 cgaagtttaa atgtgcaatt agaatatatg tataacaata ttaaacacgc ggtaaaacga
    12001 agtcagtcaa ttcaaactga tttcgcccac cgcgtgtttt taacatagct taataattaa
    12061 taagcattaa tgttcaattt atatggttgt tttccaataa taaacctaaa gatatagaat
    12121 tcacgcaata tcatgccgac acctatacat aatcctaaaa tgaatagtag tgatatcgct
    12181 agaaagacca ttgtattatc ctcaaatata tttgtatatg caaacaatga gtctagaatg
    12241 attggatgta ataaataaat aaagaatgag aaagcactaa tcatttgaat cgtattaaat
    12301 aacattgttt taaaatgcgt gcaaataccc aagataacaa taaacataat actattatat
    12361 ggtgttaatg aatatgaaaa gctggtaacg ttccaatagt ctccatttgc taacgcaata
    12421 aacacaaaat aagtagctac agctaataca atcataataa ctaaataacg ttctaagaaa
    12481 tttaatacac gttcgtagtt ataacccata tatgcaccta agaagaaata aaaaatccat
    12541 ccgaatatta tagtattttc acttaatgga taatagtgta gcacggtatc gtgaaacgct
    12601 gtgttgttcg taaagtaata taaaaatgat tgctgtaaaa taaaagataa caataataat
    12661 attttactgt tgaataggtt atagttaatt ttaaaaatga tataactcaa aataaagaat
    12721 tgcatgataa caacgataaa atagccatac cattgaccta ataggacatt ttcaatgaat
    12781 tgtttattga aacttgaatc tgttaataat gattcactat aactgtaaaa caatcccatt
    12841 aatatgtaag gaataagtat atattttacg cgtgtagtta agtatctata ggtgactttt
    12901 tggtaattca aggttgtcag taactgtgac aagataataa agcaaggtgt accaaaaatc
    12961 acaatattac gaatgtaaaa ttgtaacact aaggatccac cctccatatt ttcatgtttt
    13021 aaagtaattt gtgtaagtaa atgtgtgata ataataattg cacatataat agcacgtaaa
    13081 tatacgagtt caagtctaat ctttttcatg gaatccgtcc catctcttaa ttaaatgctc
    13141 aaaagcatca tcactaatta atattctagg gatgtaataa tcattggagt tcggagtgac
    13201 tgctttttcc tctaatgaaa aaccgtattt taacccagct tttttgatta ccggtaattt
    13261 atcgtcattc atcaagccat aaggataggc tatagttttc tgcgactttt taaagttttt
    13321 agttagatat ttttcacttt tgtttaaatc ttttatgatt gtagcttcag aagctttcat
    13381 taattttgac ttattatttt tagataagtt atgcaaatcg tgggtatgtg tttcaaattc
    13441 ccataaccca gttttataca tttcttttag ttctttttta ctaatcatat cgaggttgtg
    13501 aaagttttct tccccaacat gacctgtgat aataaaccca gttgccggta ttttatattt
    13561 ttttaagatt ggataagcat tttcataaat agtttcatcc atatcatcaa agttaa
//
LOCUS       DAXWFK010000061        13602 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.061, whole genome shotgun sequence.
ACCESSION   DAXWFK010000061 DAXWFK010000000
VERSION     DAXWFK010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13602
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.061"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            99..1307
                     /gene="gltS"
                     /locus_tag="ACGEXS_001371"
     CDS             99..1307
                     /gene="gltS"
                     /locus_tag="ACGEXS_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFF8012387.1"
                     /translation="MIELNAITTLCLACILYLLGKAIVNHVNFLKRICIPAPVIGGLI
                     FAILVAALDSFGMVKIKLDASFIQDFFMLAFFTTIGLGASLKLFKLGGKVLLLYFMFC
                     AIISVIQNIVGVSLAKVLNIKPLLGLTAGSMSMEGGHGNAAAYGKTIQDLGIDSALTA
                     ALAAATLGLVFGGLIGGPVVKFLIKRYNLKPQHSDDTFKDYSQVAYNEHLHSKFNATE
                     VFFIQFTIVVFCMAVGSYFSHLFTAQTGINVPIYVGSLFVAVIVRNISESFNFNIVDL
                     KITNQIGDVALGIFLSLALMSIQLIEIYKLAIPLIIIVLVQVVVMILFAVLILFRGLG
                     KDYDAAVMVGGFIGHGLGATPNAMANLDVITKKYGNSPKAYLVVPIVGAFLIDLIGVI
                     VIMGFIQWFS"
     gene            complement(1355..2404)
                     /gene="fni"
                     /locus_tag="ACGEXS_001372"
     CDS             complement(1355..2404)
                     /gene="fni"
                     /locus_tag="ACGEXS_001372"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFF8012388.1"
                     /translation="MSDFQREQRKNEHVEIAMAQSDAMHSDFDKMRFVHHSIPSINVN
                     DIDLTSQTPDLTMAYPVYINAMTGGSEWTKNINEKLAVVARETGLAMAVGSTHAALRN
                     PRMAETFTIARKMNPEGMIFSNVGADVPVEKALEAVELLEAQALQIHVNSPQELVMPE
                     GNREFVTWLDNIASIVSRVSVPVIIKEVGFGMSKELMHDLQQIGVKYVDVSGKGGTNF
                     VDIENERRANKDMDYLSSWGQSTVESLLETTAYQSEISVFASGGLRTPLDAIKSLALG
                     AKATGMSRPFLNQVENNGIAHTVAYVESFIEHMKSIMTMLDAKNIDDLTQKQIVFSPE
                     ILSWIEQRNLNIHRG"
     gene            complement(2435..3382)
                     /gene="corA"
                     /locus_tag="ACGEXS_001373"
     CDS             complement(2435..3382)
                     /gene="corA"
                     /locus_tag="ACGEXS_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFF8012389.1"
                     /translation="MALKIRYQTTYEPFKVVDDIKEIPKDATIVWYDFDEPNEQENEW
                     FKAHFNFNDLEVDDAINGMPRAKYKSYKDYQYLVFHSIMGSNYSPIALNIFIQDNVLV
                     TYHHQTLESLNKVVYKYMNTLDAELDCADVVILILDMMVDKYFNFVYALEDSVYHFED
                     RHVDDRFNKMVMDSVFKLRSDLIKVKRVLFPMQELIDTMKQNGDLIIDNKHSLYIQHI
                     DDHLIKQRNIIRTAQEMTNEIRENFESYTSFRMNSIMQVLTLVSVIFSPLTFIAGIYG
                     MNFVNMPALHLHYGYYICLAVMFVIAVVLIIFFRRKKWF"
     gene            3479..3970
                     /locus_tag="ACGEXS_001374"
     CDS             3479..3970
                     /locus_tag="ACGEXS_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HFF8012390.1"
                     /translation="MVIIKQLFTHTQTVTSEFIDHNNHMHDANYNIIFSDVVNRFNYS
                     HGLSLKERENLAYTLFTLEEHTTYLSELSLGDVFTVTLYIYDYDYKRLHLFLTLTKED
                     GTLASTNEVMMMGINQHTRRSDAFPESFSTQIAHYYKNQSTITWPEQLGHKIAIPHKG
                     ALK"
     gene            3967..4386
                     /locus_tag="ACGEXS_001375"
     CDS             3967..4386
                     /locus_tag="ACGEXS_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012391.1"
                     /translation="MTDALQQKIHIELLDLLDDVKFELTELNAQKGLYINGPANQLLK
                     RGVHMAYVQGQKQAIDNIMTIVEQQLEDQHFLEHYDKFQNEVAHRNYDKTANFAELSD
                     IPRQFDNFLDQFYQIKGQYFIITHINTLIGDFHSEAH"
     gene            complement(4494..5414)
                     /locus_tag="ACGEXS_001376"
     CDS             complement(4494..5414)
                     /locus_tag="ACGEXS_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFF8012392.1"
                     /translation="MIRNRVMNSVVNKYLLHNRSIMFKNDQDVERFFYKREIENRKKH
                     KQPSTLNVKANLEKLSLDDMQVFRFNFRHQIDKKILYIHGGFNALQPSPFHWRLLDKI
                     TLSTLYEVVLPIYPKTPEFHIDDTFQAIQRVYDQLVSEVGHQNVVVMGDGSGGALALS
                     FVQSLLDNQQPLPNKLYLISPILDATLSNKDISDALIEQDAVLSQFGVNEIMKKWANG
                     LPLTDKRISPINGTIEGLPPVYMFGGGREMTHPDMKLFEQMMLQHHQYIEFYDYPKMV
                     HDFPIYPIRQSHKAIKQIAKSIDEDVTQNN"
     gene            5622..6260
                     /locus_tag="ACGEXS_001377"
     CDS             5622..6260
                     /locus_tag="ACGEXS_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012393.1"
                     /translation="MTNPNQRLEPFDETFQQPNIHRGKRYGKKKRSLVSMIIQIIVVI
                     LTAIAGYSMWKQPIFNIVFTKESVDFDGIKNFNETIGKMHQLNINLGNINDLQQGIDR
                     LILVFNGFFILCIVSLVITILTIVFNRTVLKVVNILVLTIMLVITLYFSYIIKALAQE
                     IADVFKKYYLNVPPDQVITEADAIHNGIILLGCSIGLLVISLFFRNRMPRLK"
     gene            complement(6506..8437)
                     /locus_tag="ACGEXS_001378"
     CDS             complement(6506..8437)
                     /locus_tag="ACGEXS_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HFF8012394.1"
                     /translation="MTTTFIISYIILALIIVGVINLFLIRSRKKGKRQQKEQQFTTRQ
                     SNQSKFKASDLDKTTDQSTQRMTHEELRVDNQDDHSQVSLNGYTKGSEKDQEAFTNNK
                     DEEAVAAKNPESEEYKVNEKIKKEHKNFIFGEGVSRGKILAALLFGMFIAILNQTLLN
                     VALPKINTEFNISASTGQWLMTGFMLVNGILIPITAYLFNKYSYRKLFLVALVLFTIG
                     SLICAISMNFPIMMVGRVLQAIGAGVLMPLGSIVIITIYPPEKRGAAMGTMGIAMILA
                     PAIGPTLSGYIVQNYHWNVMFYGMFIIGIIAILIGFVWFKLYQYTTNPKADIPGIIFS
                     TIGFGALLYGFSEAGNKGWGSVEIETMFAIGIIFIILFVIRELRMKSPMLNLEVLKFP
                     TFTLTTIINMVVMLSLYGGMILLPIYLQNLRGFSALDSGLLLLPGSLIMGLLGPFAGK
                     LLDTIGLKPLAIFGIAVMTYATWELTKLNMDTPYMTIMGIYVLRSFGMAFIMMPMVTA
                     AINALPGRLASHGNAFLNTMRQLAGSIGTAILVTVMTTQTTQHLSAFGEELDKTNPVV
                     QDHMRELASQYGGQEGAMKVLLQFVNKLATVEGINDAFIVATIFSIIALILCLFLQSN
                     KKAKATAQKLDADNSINHE"
     gene            complement(8450..9097)
                     /locus_tag="ACGEXS_001379"
     CDS             complement(8450..9097)
                     /locus_tag="ACGEXS_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFF8012395.1"
                     /translation="MKKMILINVITVVVLLAIGIAGFYFWNKTTSYVTTDNAKVNGDQ
                     IKIASPASGQIKSLNVKQGDKLDKGDKVAIVTVQGQDGETKDMDLKMPQKGTIAKLDG
                     MEGSMVQAGNPIAYAYNLDDLYVTANIDEKDIKDVEVGKDVDVTIDGQKASIKGKVDS
                     IGKATAASFSLMPSSNSDGNYTKVSQVIPVKITLESEPSKQVVPGMNAEVKIHKN"
     gene            9218..9769
                     /locus_tag="ACGEXS_001380"
     CDS             9218..9769
                     /locus_tag="ACGEXS_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8012396.1"
                     /translation="MKRQAKIEIQNALVDLMAEYPFQEISTKMICAYCNINRSTFYDY
                     YKDKFDLLDTINSKHKEKFQFLLSALHHNFENIKQDKLKLYKFFIIIAKYIKHNEQFF
                     KDILVTYPMKTLFIDYINLARDYYQQIMNDYSTTVTNKQLYVTYIIGGQAGVFINWLS
                     NGCNESPEEVADILLANTIKLQN"
     gene            complement(9999..11207)
                     /locus_tag="ACGEXS_001381"
     CDS             complement(9999..11207)
                     /locus_tag="ACGEXS_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HFF8012397.1"
                     /translation="MNDLTHFNKRSPIFIIILGSLTAIGALSIDMFLPGLPDIRHDFQ
                     TTTSNAQLTLSMFMIGLAFGNLFAGPISDSTGRRKPLIIAMIIFTLASLGIVFVHNIW
                     LMVALRFLQGVTGGAAAVISRAIASDMYSGNELTKFMALLMLVNGIAPVVAPTIGGII
                     LNYSVWRMVFVILTIFGFVMVIGSLLKVPESLTVTNRESSSGLKTMFKNFKILLKTPR
                     FVLPMLIQGMTFVILFTYISASPFIIQKIYGMTAIQFSWMFAGIGITLIISSQLTGYL
                     VDFIDSQKLMRGMTMIQIIGVILVTIVLLNHWNFWILAIGFIILIAPVTGVATLGFTI
                     AMDESSSGRGSSSSLLGLVQFLFGGVASPLVGVKGEDNPIPYIIIIIATAVILIILQI
                     YNMKVFKTNR"
     gene            complement(11317..11445)
                     /locus_tag="ACGEXS_001382"
     CDS             complement(11317..11445)
                     /locus_tag="ACGEXS_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012398.1"
                     /translation="MEYKQHPFKVFTISVNYFEGIVFCGKIYEVLRFISFYTFYKL"
     gene            complement(11476..12858)
                     /gene="tcaA"
                     /locus_tag="ACGEXS_001383"
     CDS             complement(11476..12858)
                     /gene="tcaA"
                     /locus_tag="ACGEXS_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein TcaA"
                     /protein_id="HFF8012399.1"
                     /translation="MKSCPKCGQQAQDDVQICTQCGHKFDSRQALYRKSTDEDIQTNN
                     IKMRKMVPWAIGFFILILIIILFFLLRNFNSPEAQTKILVNAIENNDKQKVATLLSTK
                     DNKVDSEEAKVYINYIKDEVGLKQFVSDLKNTVHKLNKSKTSVASYIQTRSGQNILRV
                     SKNGTRYIFFDNMSFTAPTKQPIVKPKEKTKYEFKSGGKKKMVIAEANKVTPIGNFIP
                     GTYRIPAMKSTENGDFAGHLKFDFRQSNSETVDVTEDFEEANISVTLKGDTKLNDSSK
                     KVTINDHEMAFSSSKTYGPYPQNKDITISASGKAKDKTFTTQTKTIKASDLKYNTEIT
                     LNFDSEDIEDYVEKKEKEENSLKNKLIEFFAGYSLANNAAFNQSDFDFVSSYIKKGSS
                     FYDDVKKRVSKGSLMMISSPQIIDAEKHGDKITATVRLINENGKQVDKEYELEQGSQD
                     RLQLIKTSEK"
     gene            complement(13098..13553)
                     /locus_tag="ACGEXS_001384"
     CDS             complement(13098..13553)
                     /locus_tag="ACGEXS_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFF8012400.1"
                     /translation="MVKHLQDHIQFLEQFINNVNALTAKMLKDLQNEYEISLEQSNVL
                     GMLNKEPLTISEITQRQGVNKAAVSRRIKKLIDAKLVKLDKPNLNIDQRLKFITLTDK
                     GRAYLKERNAIMTDIAQDITNDLNSEDIENVRQVLEVINHRIKTYSNHK"
BASE COUNT         4438 a         2395 c         1922 g         4847 t
ORIGIN      
        1 tgatttgttg catgtataat gttaaaagcg cccttttata acgcttacat ataaaagctt
       61 atttagggag agggatattc aacaaggggg atttgaaaat gatagaactt aatgcaatta
      121 caacattatg tttagcttgt atcctttatt tacttggtaa ggctatcgtt aatcacgtta
      181 attttttaaa acgtatttgt ataccagcac cagtgattgg cggcttaatc tttgctattt
      241 tagttgcggc tttggattca tttggcatgg ttaagattaa attagatgct tcattcattc
      301 aagatttctt catgttagca ttctttacga caatcggtct tggtgcatca ttgaaattat
      361 ttaaattagg tggcaaagtc ttgctattat actttatgtt ttgtgctatc atttcagtca
      421 ttcaaaacat agttggtgta tcactagcaa aagtattaaa tattaaacct ttgttaggat
      481 taacagcagg ttccatgtct atggaaggcg gtcatggtaa tgctgctgct tatggtaaga
      541 caattcaaga tttaggtatt gattcggcac tgacagcggc tcttgcagct gcaactttag
      601 gtcttgtatt tggagggctt atcggtggtc cagttgtaaa atttttaatc aaacgttata
      661 acttaaagcc tcaacatagt gacgatacat ttaaagatta tagccaagta gcatataacg
      721 aacatttaca tagtaaattt aatgccactg aagtattctt cattcaattt acaatcgttg
      781 tattctgtat ggcagttgga agttatttca gtcatttgtt tacagctcaa acagggatta
      841 atgttccaat ttacgttggc tcattatttg tagctgttat tgtccgaaat atctctgaaa
      901 gttttaattt taatattgta gatttaaaaa ttactaatca aattggcgat gtcgcattag
      961 gtattttctt atctcttgcg ctaatgagca ttcaattaat cgaaatttat aaacttgcta
     1021 tacctcttat tattatcgtt ttagttcaag ttgtcgttat gattttattt gctgttttaa
     1081 ttttatttag aggtttagga aaagattatg atgctgcagt aatggtaggt ggttttatcg
     1141 gtcatgggct tggtgcaacg ccaaatgcca tggcaaattt agatgttatt actaaaaaat
     1201 atggaaactc acctaaagca tatttagttg tacctattgt tggtgcattc ttaatcgatt
     1261 taattggtgt tatagtcatt atgggattca tacaatggtt tagttaaaca ccaaactcat
     1321 aaataaaaga ggaggccttc gcctcctctt ttatttatcc tcgatgtata ttcaagttac
     1381 gttgttctat ccatgacaat atttccggac taaatacgat ttgtttttgt gttaagtcgt
     1441 caatattttt agcatctaac atcgtcatta ttgatttcat gtgttcaata aatgattcta
     1501 cataagctac tgtatgtgca atgccattat tttcaacttg atttaaaaac ggacgtgaca
     1561 taccagttgc ctttgcacca agtgctaaac ttttaattgc atcgagtggt gtacgtaaac
     1621 caccactcgc gaaaactgaa atttcgcttt gataagccgt tgtttcaagt aatgactcaa
     1681 ctgtagactg tccccatgat gataagtaat ccatatcttt atttgcacga cgttcatttt
     1741 caatatctac aaagttagta ccacctttgc cactaacatc gacatacttg acgcctattt
     1801 gttgtaagtc atgcattaat tctttgctca taccaaatcc aacttctttt ataatgactg
     1861 gaacagacac tcgtgataca atcgacgcta tattatctaa ccaagtcaca aattcacgat
     1921 tcccttcagg cataactaat tcttgaggag aattaacatg gatttgtaac gcttgtgcct
     1981 caagtaattc aactgcttcc aaagcctttt ctactggtac gtccgcacca acattgctaa
     2041 aaatcatgcc ttcaggattc atttttcgcg caatcgtaaa cgtctcagcc atgcgtggat
     2101 ttctcaatgc cgcatgtgtt gatccaactg ccatcgctaa gccagtttct cttgcaacta
     2161 cagctagctt ttcattgatg tttttcgtcc actcgctacc acccgtcatt gcattaatat
     2221 aaaccggata tgccatcgtt aagtcaggcg tctgtgatgt caaatcgata tcatttacat
     2281 taattgatgg gatagaatga tgcacaaaac gcatcttatc aaaatctgaa tgcattgcgt
     2341 cagattgggc cattgctatt tcaacatgtt cattttttct ctgttctctt tgaaaatcac
     2401 tcatgattaa acctaccttt tcgtcatttc taatttaaaa ccatttttta cgtctgaaaa
     2461 agatgattaa tacgacagct ataacaaaca ttacagctaa gcaaatataa tatccataat
     2521 gtaaatgtaa tgccggcata tttacaaagt tcataccata aatcccagct atgaatgtta
     2581 acggtgaaaa tataactgat actaatgtca gtacttgcat aatactattc attctaaatg
     2641 acgtgtatga ctcaaaattt tctcgtattt cgtttgtcat ttcttgagca gtacgaatga
     2701 tattacgttg cttaatcaag tggtcatcga tatgttgaat gtatagcgaa tgtttattat
     2761 ctataatcaa atcaccattt tgtttcattg tatcaattag ctcttgcata ggaaacagta
     2821 cacgttttac tttaatcaaa tccgaacgta acttaaagac actatccatg accattttat
     2881 taaagcgatc atctacatgg cggtcttcaa aatgataaac actatcttca agtgcatata
     2941 caaagttgaa atatttatca accatcatat ctaaaattaa tatgacgaca tctgcacaat
     3001 ctaattctgc atctaatgta ttcatatact tatagactac tttatttaat gattccaacg
     3061 tttgatgatg atatgttact aatacattgt cttgtataaa aatatttagt gctattggtg
     3121 aatagtttga ccccataata ctatggaata ctaagtattg ataatcttta taagatttat
     3181 atttagctcg tggcataccg ttaattgcat catccacttc taaatcatta aaattaaaat
     3241 gtgctttaaa ccattcattt tcttgttcat tcggttcatc aaaatcatac caaacaatag
     3301 tcgcatcttt tggtatctct ttgatatcat caactacttt aaacggttca tatgtagttt
     3361 gataccgtat ctttaaagcc atcgatactc cccctaaata acgaattctc tattatttta
     3421 tcatgaatta aataacgtgt atgtcttaat ttattttagt atgatagtca ctaaggagat
     3481 ggttattatc aaacaacttt ttacacatac tcaaaccgta acatctgaat tcattgacca
     3541 taacaatcat atgcatgatg caaattataa tatcattttt agtgacgtcg tgaatcgttt
     3601 taattacagc cacggtcttt ctttaaaaga acgcgaaaat ttagcatata cgctatttac
     3661 actagaagaa catacgacat acctctcaga attgtctctt ggcgatgtat ttactgttac
     3721 tttatatatt tatgattacg attataagcg gttgcattta tttttaacat taactaaaga
     3781 agatggtaca ctagcatcaa caaatgaagt aatgatgatg ggaattaatc agcacacacg
     3841 tcgttctgat gcttttcctg aatcattttc aacacaaata gcacactatt ataaaaatca
     3901 atcaactatc acttggcctg aacaattagg acataaaata gcaattccac acaaaggagc
     3961 attaaaatga cagatgcatt acaacaaaag attcatatcg aattactaga tttattagat
     4021 gatgttaagt ttgaattaac agaattaaat gcacaaaaag ggttatacat taacggacca
     4081 gcaaatcagc tacttaagcg tggcgtgcat atggcttatg ttcaaggaca aaagcaagcc
     4141 atcgataata ttatgactat tgtggaacaa cagcttgaag atcaacattt cctagaacat
     4201 tatgataaat ttcaaaatga ggttgctcat cgcaattatg ataaaacagc taactttgca
     4261 gaacttagtg atattccacg tcaattcgat aattttttag atcaatttta tcaaattaaa
     4321 gggcaatact ttatcatcac acatatcaat acacttattg gtgattttca ctcagaagct
     4381 cattaacaat tagtctatat aacccttgct atattttcaa aaacaaaacc caattacgtt
     4441 ttcatgtcaa atatcatctt gcatgaaatc gtaactgggt catttatatg ttattagtta
     4501 ttttgtgtta catcctcatc tatcgatttg gcaatttgtt taatagcttt atgtgattgt
     4561 ctaattggat aaattggaaa atcatgtacc atcttaggat aatcataaaa ctcaatgtat
     4621 tgatgatgtt gcaacatcat ttgttcaaat agcttcatat caggatgtgt catttcacgt
     4681 ccaccaccaa acatataaac tggtggcaat ccttctattg tgccattaat tggcgatatg
     4741 cgcttatctg ttaatggtag gccattcgcc cattttttca taatctcatt gacaccaaac
     4801 tgacttagaa ccgcatcttg ttcgattaag gcgtccgaaa tatctttatt agatagtgtt
     4861 gcatctaaaa ttggtgagat taaatacaat ttattcggta atggctgttg attatctaaa
     4921 agagattgta caaaggataa tgccagtgca ccacctgaac catcacccat gactacgaca
     4981 ttttgatgtc ctacttcaga tactaattga tcataaacac gttgtatcgc ttggaaagta
     5041 tcgtcaatat gaaactctgg tgtctttgga tagataggca gtacaacctc atataatgta
     5101 cttaaagtga ttttatccaa caatctccaa tggaacggtg atggttgtag tgcattgaat
     5161 ccaccgtgaa tatataaaat tttcttatca atttgatgtc tgaaattaaa gcgaaagact
     5221 tgcatatcat ctaatgacaa tttttctaaa tttgctttaa catttaatgt tgaaggctgc
     5281 ttatgttttt ttctattttc aatttctctt ttataaaaaa atctttcaac atcttgatca
     5341 tttttaaaca taatcgagcg attgtgaagc aaatatttat tgacaacgct attcataaca
     5401 cggtttctaa tcaatgtctt aacctacctt tatatatttt atgtatccaa tgattgtcta
     5461 tcccctacat tctttgccaa aaaaagtata taatgtagaa gatattttct ttttcacttt
     5521 caaatttaag actacaattg aacagtgatt tttcatcatt ataacagaca actagacata
     5581 ttgataagta aagaaaagaa ctttatacgg aggtaccttg catgacaaat ccaaatcaac
     5641 gattagaacc atttgatgag acatttcaac aaccgaatat tcatcgtggt aagcgatatg
     5701 gtaagaaaaa acgttcattg gtaagcatga ttattcaaat cattgttgtt atattaaccg
     5761 caatcgctgg atacagcatg tggaaacaac ccatttttaa tattgtcttc accaaagaat
     5821 ctgtagattt tgatggcatt aaaaatttta acgagacaat tggaaaaatg catcaattaa
     5881 atattaattt gggtaatatc aatgatttac aacaaggaat cgatcgctta attttagtct
     5941 ttaacggttt ctttatttta tgtatcgtta gcctagtcat aacaatactg acaattgtat
     6001 ttaatcgcac agttctaaaa gtagtaaata ttttagtgtt aacaattatg ttagtcatta
     6061 cattgtattt cagttatatt attaaagcac tcgcacaaga aattgcagat gttttcaaaa
     6121 aatattattt aaatgtacca ccagatcaag tgattacaga agctgacgct attcataacg
     6181 gcatcatttt acttggctgt agcatcggtt tacttgttat aagcttattc ttccgtaatc
     6241 gcatgcctcg tttaaaataa tgttatatag tagggattgg gacagaaatg ttattttcac
     6301 aaaattcatt tcgttgaccc acttcctgct ttttctttat taatgaccta ctacgtcaat
     6361 gccaaatttt aatataattc ctctaacatt ctataaatca acaaataaaa aaagtatcga
     6421 taaccatatt tggcaccgat actttaaaaa tcatgtcccc acaacataat ttttgattag
     6481 tcacacttca attaatttat tttatttatt catgattgat actattatct gcatctaact
     6541 tttgagctgt agcttttgct tttttattac tttgtaaaaa taaacataaa attaaggcga
     6601 tgatgctaaa tatcgttgca actataaatg catcattaat accttcaacc gttgctagtt
     6661 tatttacaaa ttgtagtaac actttcattg cgccttcttg tccgccatat tgtgatgcta
     6721 attcacgcat atgatcttgt acaacaggat tcgttttatc taactcttcc ccaaaagctg
     6781 ataagtgttg tgtagtttgt gttgtcatta cagtaactaa gattgctgta cctatagagc
     6841 ctgctaattg acgcatcgta tttaagaaag cattaccatg agaggcaagt cgtcccggta
     6901 acgcattaat agctgcagtt accattggca tcattataaa tgccatacca aatgaacgaa
     6961 gtacatagat acccatgatt gtcatatatg gtgtatccat atttaattta gttaattccc
     7021 atgttgcata agtcattaca gcaataccaa agatagctaa tggttttaaa ccaatagtat
     7081 ctaacaattt acctgcaaat ggtcctagta gacccataat tagagaacca ggtaataata
     7141 acaatccgga atctaatgct gagaatccgc gtaaattttg taaataaatc ggtaataaaa
     7201 tcataccacc atataaactt aacattacaa ccatattaat aattgttgtt aatgtaaatg
     7261 ttgggaattt caatacttct aaattcaaca ttggtgattt cattcttaat tctctaataa
     7321 cgaatagaat aataaagata ataccaatcg caaacattgt ttctatctct actgaacccc
     7381 aacctttgtt gccagcttct gagaaaccat ataacaaagc accaaaacca atcgtactaa
     7441 aaatgatacc tgggatatca gctttagggt ttgttgtata ttgatataac ttaaaccata
     7501 caaaaccaat taaaatagcg ataatcccga taatgaacat accgtaaaac atcacattcc
     7561 aatggtaatt ttgtacaata taacctgata atgttggacc aattgcaggt gctaaaatca
     7621 ttgcgatacc cattgtaccc atggcagcac cacgtttttc aggtggataa attgtaataa
     7681 taacaattga acctaatggc attagtacac ctgcaccaat ggcttgtaat acacgtccaa
     7741 ccatcatgat tgggaaattc attgaaatcg cacagattaa tgaaccaatt gtaaagagta
     7801 ctaacgcaac taaaaataat tttcgatatg aatatttatt aaatagatac gccgtaattg
     7861 gtattaaaat accgtttact aacatgaatc ccgtcatcaa ccattgccct gttgacgcag
     7921 aaatattaaa ttccgtatta atttttggta aagcaacatt taataatgtt tggtttaaaa
     7981 tcgcaataaa cataccgaat aataatgccg ctaatatttt accgcgtgaa acaccttcac
     8041 caaaaataaa gtttttatgt tcttttttta ttttttcatt cactttatat tcttctgatt
     8101 caggattttt agcagcaact gcttcctcat ccttattatt agtgaatgct tcttgatctt
     8161 tctcagaccc ctttgtgtaa ccatttagac taacttggct atgatcatct tgattgtcaa
     8221 cacgcaactc ttcatgcgtc atacgttgtg tcgattgatc agttgttttg tctaaatcac
     8281 tagctttaaa tttagattga tttgattgac gtgtcgtaaa ttgttgttcc ttttgttggc
     8341 gtttgccttt ttttcttgat cttattaaaa ataaattgat aaccccaaca ataatgagcg
     8401 ctaaaataat gtagctaata atgaaggtcg tagtcattta atgaccccct taatttttat
     8461 ggatttttac ttcagcgttc attccaggaa caacttgttt agacggttct gattctagag
     8521 tgattttaac aggtattact tgagaaactt tagtgtagtt accatcacta tttgatgatg
     8581 gcattaatga aaagcttgca gcagttgctt ttccaatact atcaacttta cctttaatag
     8641 aagctttttg accgtcaata gtcacatcaa catctttacc tacttcaaca tctttaatat
     8701 ctttttcgtc aatatttgct gttacatata aatcatctaa attgtatgca taagcgattg
     8761 ggttaccagc ttgcaccatt gaaccttcca taccatctaa tttggcaatt gtaccttttt
     8821 gaggcatttt aagatccata tctttcgttt cgccatcttg accttgtaca gtaacaattg
     8881 ctactttgtc acctttatcg agtttgtcac cttgtttaac attaagtgat ttaatttgtc
     8941 cagatgcagg acttgctatt ttaatttgat cgccatttac ttttgcatta tcagttgtta
     9001 catagcttgt tgttttattc caaaaataaa agccagcaat cccaattgct aacagtacaa
     9061 caaccgtaat gacattaatt aatatcatct ttttcatgaa acatttcctc ctatttgctt
     9121 ttaaaattca ctttatttat tatagagaca tgataactaa aaaaacacca acaattttta
     9181 taagagtgtt ggttaaaatt cacaaaggtg taacaatatg aaacgacaag ccaaaataga
     9241 aattcaaaac gcacttgttg atttgatggc tgaatatcct tttcaagaaa tatccactaa
     9301 aatgatttgc gcgtattgta atattaatcg ttctactttt tatgattatt acaaagataa
     9361 atttgattta ttggatacca tcaactcaaa gcataaggaa aaattccaat ttcttctaag
     9421 cgcgctacat cataatttcg aaaatattaa acaggataag cttaaacttt ataaattctt
     9481 cattattatt gctaagtata tcaagcacaa tgagcagttt ttcaaagaca ttttagttac
     9541 ctatccaatg aaaacattat tcatagacta tataaattta gcgagagatt actatcaaca
     9601 aattatgaat gactatagta ctacagtaac caataaacag ctttatgtta catatatcat
     9661 aggcggtcaa gctggggtgt ttattaattg gctttcgaat ggttgtaatg aatcacctga
     9721 agaagttgct gacattttat tggctaatac gattaaactt caaaactaat aatacggttt
     9781 cattctaata aagaaatgct ataaaacaac tagataaagt taaaccaaat ggatagataa
     9841 tagtacatat gtaacggtgc tatcaatata aaaatgccga gatttccaca taataatctc
     9901 ggcattttta tttttgatgc taaattcata ggtctatgaa tagctcaagt tttgtttttt
     9961 aatttttcaa ttcaatataa tagaagaaac tatgtttatc atcgatttgt tttaaatacc
    10021 ttcatattat aaatctgtaa aataattaat ataactgctg tagctataat aataattatg
    10081 taaggtatag gattatcttc accttttacc cccactaatg gcgatgcaac accaccaaat
    10141 aaaaactgta ctaaacctaa taaacttgat gaactccctc taccactact actttcatcc
    10201 atagctattg taaaaccaag tgtcgcaaca cctgtcactg gtgctattaa aatgataaat
    10261 cctatcgcta aaatccaaaa gttccaatga ttaagcaata caatcgtcac taaaatcaca
    10321 ccgataatct gaatcatcgt catgcctctc atcaattttt gtgagtctat aaaatcaaca
    10381 aggtacccag taagttgact tgatataatg agcgtaatac caattcctgc aaacatccaa
    10441 ctaaattgta ttgcagtcat gccataaatt ttttgaataa taaacggaga cgcagaaata
    10501 taagtaaata atattacaaa tgtcatacct tgaatgagca ttggtaatac aaaacgtggc
    10561 gtcttcaata atattttgaa gtttttaaac attgtcttta atccactact tgactcacga
    10621 tttgtcactg ttaatgattc aggtactttt aataaagaac ctatgaccat gacgaagcca
    10681 aagatagtca gaatgacaaa gaccatacgc cagacagaat aatttaaaat tatgccccct
    10741 attgttggcg caacaactgg tgcaatacca ttaacaagca tcaataatgc cataaactta
    10801 gttagttcat taccactata catatcactt gctatagctc ttgaaataac tgctgctgcg
    10861 ccacctgtca ctccttgaag aaatcttaat gcaaccatca gccaaatatt atgtacaaaa
    10921 acaataccta aacttgctaa tgtaaaaata atcatagcta taataagcgg ctttctgcgc
    10981 ccagttgaat ctgaaattgg accagcaaac aaattaccaa acgctaaacc aatcataaac
    11041 atcgataatg ttaattgtgc gttagacgtc gttgtttgaa aatcatgtct aatatctggt
    11101 aatccaggca aaaacatatc tatcgataat gctccaatgg ccgtcaaaga tcctaaaata
    11161 ataataaata tcggtgaacg cttgttaaaa tgcgttaaat cattcatatg actatctcct
    11221 ttatataatg attagtttat aactatcacg aacagaaact aaatttaaat atgctctttt
    11281 atatattagc attagtcaca agtttcatga acatcattac aacttataaa aggtataaaa
    11341 tgatataaac ctcagcactt cataaatttt tccacaaaaa acaatccctt caaagtaatt
    11401 cacactaatt gtaaatactt tgaaaggatg ttgtttgtat tccatgacac atgcatctta
    11461 tttaagtttt atactctatt tttctgatgt cttgattaat tgcaagcggt cttgcgagcc
    11521 ttgttcaagc tcatattctt tatctacttg cttaccgttt tcatttatta atcttacggt
    11581 tgcggtaatc ttatcaccat gtttttcagc atctataatt tgtggtgaac taatcatcat
    11641 taaacttcct ttagatacac gcttctttac atcatcataa aaagatgatc ctttttttat
    11701 atatgatgat acaaaatcaa aatccgactg attaaacgca gcattattcg ctaaagaata
    11761 tccagcaaag aattctatca atttgttctt caagctgttt tcttcttttt ctttcttttc
    11821 aacatagtct tcgatatctt cactgtcaaa attcaaagtt atctctgtat tgtattttaa
    11881 atcgctagct ttaatcgtct tcgtctgtgt tgtaaatgtt ttatctttcg ctttacctga
    11941 agctgaaatg gtaatatctt tattttgtgg atatggacca tacgttttgg aacttgaaaa
    12001 tgccatttca tggtcattta tagttacttt tttagaacta tcatttaatt ttgtatcgcc
    12061 ttttaaagtt acagatatat ttgcttcttc aaaatcttca gtaacatcta ccgtttcaga
    12121 attactttgt ctaaaatcaa attttaaatg gcctgcaaaa tcaccgttct cagttgattt
    12181 catagctgga attctatatg tccccggtat aaaattacct attggcgtca ctttatttgc
    12241 ttcagctata accatctttt tcttaccacc agatttaaac tcatattttg ttttttcttt
    12301 cggtttaaca attggttgct tggtaggtgc agtaaagctc atattatcga aaaagatata
    12361 acgtgtgcca tttttactta cacgtaatat attttgacca gatctggttt gaatataaga
    12421 agctacgctg gtcttactct tattcaattt atgtaccgta tttttaaggt cgctgacaaa
    12481 ttgcttaagc ccaacttcat ctttgatata gttaatgtat acttttgctt cttcagaatc
    12541 tactttatta tctttagtac ttaataatgt tgctactttt tgtttatcat tattctcaat
    12601 cgcattcact aatattttag tttgcgcttc aggtgaattg aagtttctta gtaaaaagaa
    12661 caatataata attaagatta aaatgaaaaa accaattgcc caaggcacca tttttctcat
    12721 cttgatgtta ttagtttgta tatcttcatc ggttgacttt ctatataatg cttgacgact
    12781 atcaaattta tgtccacatt gtgtacatat ctgtacatca tcttgtgctt gttgtccgca
    12841 cttcgggcaa gatttcatac tttcacctca attctgttcc tcttcaattg ttatatgtat
    12901 atcacaaaaa ttatttcatt attttaccat tattatttta caaatattat cccaagaata
    12961 caattcaaga aatgattaac tttactttag aattgtacct tattacatta tttatgaaag
    13021 tactctctca attataacta aaaagaaggt gttcaataca tacatattta gtcttatatt
    13081 tgaacacctt caaaaaccta tttatgattg ctatatgttt taatgcgatg attgatgact
    13141 tctaatactt gccttacatt ttcaatatct tcagaattta aatcattagt aatatcttgc
    13201 gcaatatctg tcataatcgc attacgttct ttcaaatatg ctctaccttt gtcagttaag
    13261 gttatgaatt tcaaacgttg atcaatattt aaatttggtt tatctaactt aactaattta
    13321 gcatcgatta actttttaat tcgtcggctt actgcggcct tatttacacc ttgtctttgc
    13381 gtgatttcac taattgtcaa aggttcttta tttaacatac ctaatacgtt agactgctct
    13441 aatgaaattt catattcatt ttgtaaatct ttcaacattt ttgcagttaa tgcgttaacg
    13501 ttatttataa actgctctaa aaattgaata tggtcttgta aatgttttac cataattatt
    13561 tctccctaat tactattttt ataaagtatc ctcagcaatc at
//
LOCUS       DAXWFK010000062        13541 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.062, whole genome shotgun sequence.
ACCESSION   DAXWFK010000062 DAXWFK010000000
VERSION     DAXWFK010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13541
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.062"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(95..1846)
                     /gene="srrB"
                     /locus_tag="ACGEXS_001385"
     CDS             complement(95..1846)
                     /gene="srrB"
                     /locus_tag="ACGEXS_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SrrB"
                     /protein_id="HFF8012401.1"
                     /translation="MMSRLNSVVIKLWLTIILIVTTVLILLSIALITFMQYYFTQETE
                     NAIREDARRISSLVEQSHNKEEAIKYSQTLIENPGGLMIINNKHRQSTASLSNIKKQM
                     LNEVVNNDHFDDVFDKGKSVTRNVTIKEKGSSQTYILLGYPTKAQKNSHSKYSGVFIY
                     KDLKSIEDTNNAITIITIITAVIFLTITTVFAFFLSSRITKPLRRLRDQATRVSEGDY
                     SYKPSVTTKDEIGQLSQAFNQMSTEIEEHVDALSTSKNIRDSLINSMVEGVLGINESR
                     QIILSNKMANDIMDNIDEDAKAFLLRQIEDTFKSKQTEMRDLEMNARFFVVTTSYIDK
                     IEQGGKSGVVVTVRDMTNEHNLDQMKKDFIANVSHELRTPISLLQGYTESIVDGIVTE
                     PDEIKESLAIVLDESKRLNRLVNELLNVARMDTEGLSVNKEVQPIAALLDKMKIKYRQ
                     QADDLGLNMTFNYCKKRVWSYDMDRMDQVLTNLIDNASRYTKPGDEIAITCDENESED
                     ILYIKDTGTGIAPEHLQQVFDRFYKVDAARTRGKQGTGLGLFICKMIIEEHGGSIDVK
                     SELGKGTTFIIKLPKPE"
     gene            complement(1827..2552)
                     /gene="srrA"
                     /locus_tag="ACGEXS_001386"
     CDS             complement(1827..2552)
                     /gene="srrA"
                     /locus_tag="ACGEXS_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator SrrA"
                     /protein_id="HFF8012402.1"
                     /translation="MSNEILIVDDEDRIRRLLKMYLERESFEIHEASNGQEAYELAME
                     NNYACILLDLMLPEMDGIQVATKLREHKQTPIIMLTAKGEETNRVEGFESGADDYIVK
                     PFSPREVVLRVKALLRRTQSTTVEQSEPHARDVIEFKHLEIDNDAHRVLADNQEVNLT
                     PKEYELLIYLAKTPNKVFDREQLLKEVWHYEFYGDLRTVDTHVKRLREKLNRVSSEAA
                     HMIQTVWGVGYKFEVKSNDEPAK"
     gene            complement(2685..3422)
                     /locus_tag="ACGEXS_001387"
     CDS             complement(2685..3422)
                     /locus_tag="ACGEXS_001387"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFF8012403.1"
                     /translation="MTKELERLQKRIANSGYTSRRKAETLISEGKVKVNGTTVTELGT
                     KVKPSDTIEVEGIKIELEDKIYILFHKPTQVITSVSDDRGRTVVTDYFKDIEARIYPV
                     GRLDYDTSGLLLLTNDGEFTNLMTHPRYQIKKKYVAKLKGYLMREEVKALEKGIELED
                     GMTQPAEVKVKKQDKDKNTTLVEITITEGRNRQVRRMFEHFGHQVTKLSRIEYGPLNV
                     VGLNAGEGRVLTPHEVKVMRHLAEHGK"
     gene            complement(3415..3957)
                     /gene="scpB"
                     /locus_tag="ACGEXS_001388"
     CDS             complement(3415..3957)
                     /gene="scpB"
                     /locus_tag="ACGEXS_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFF8012404.1"
                     /translation="MDNHGILESLLFTAGDEGLDEKQLLEILDMSKDQLVELIENYSS
                     HGLMIQRFGMTYVLTTKKEAATYIEQLIEQKSQMKLSQAAMEVLSIIAYNQPLSRSDI
                     ELIRSINSDGAVKTLIAKGLVEAKVVNEQRSQQLITTDLFLNVFGISNIEDLPTTEED
                     DEEMDAFFSNLVNQKGENND"
     gene            complement(3950..4681)
                     /locus_tag="ACGEXS_001389"
     CDS             complement(3950..4681)
                     /locus_tag="ACGEXS_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFF8012405.1"
                     /translation="MYEVKLDAFNGPLDLLLHLIQKFEIDIYDIPMQALTEQYMQYVH
                     AMKQLEINIASEYLVLASELLMIKSKMLLPQSTSDMDVDDDPREDLVGRLIEYQNYKE
                     YTAILNDMKEERDFYFTKRPTDLSHLETDESWDPNHTIDLTELIVAYQRVKNRVELNT
                     PKSVEIRKETFTIQQATEQVTSRLKDKDHFNFFSLFTFSEPIEQVVTHFLAILEMSKA
                     GIINIEQQRNFEDINIIRGVNYHFG"
     gene            4773..5279
                     /locus_tag="ACGEXS_001390"
     CDS             4773..5279
                     /locus_tag="ACGEXS_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HFF8012406.1"
                     /translation="MQQALINFYYQFHTKQHYFLCHDILEDAWKAENNYSKQDAVVSL
                     ILFATACYHYRRNNLKGAYKSFNKSKEIIQNAKDRDALYLNLNDYQLLIEQQIAKLNA
                     AKPFSSVILPITPVFERIIKANYPDYDYNQETATDPFIVDHHMRRDRSEVIAAKEEAI
                     QLRKHRRN"
     gene            complement(5357..6244)
                     /gene="xerD"
                     /locus_tag="ACGEXS_001391"
     CDS             complement(5357..6244)
                     /gene="xerD"
                     /locus_tag="ACGEXS_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFF8012407.1"
                     /translation="METIIEEYLRFIQIEKGLSSNTIGAYRRDLKKYQDYMTEHHISH
                     IDFIDRQLIQECLGHLIDQGQSAKSIARFISTIRSFHQFAIREKYAAKDPTVLLDSPK
                     YDKKLPDVLNVDEVLALLETPDLNKINGYRDRTMLELLYATGMRVSELIHLELENVNL
                     IMGFVRVFGKGDKERIVPLGDAVIEYLTTYIETIRPQLLKKTVTEVLFLNMHGKPLSR
                     QAIWKMIKQNGVKANIKKTLTPHTLRHSFATHLLENGADLRAVQEMLGHSDISTTQLY
                     THVSKSQIRKMYNQFHPRA"
     gene            complement(6293..6742)
                     /locus_tag="ACGEXS_001392"
     CDS             complement(6293..6742)
                     /locus_tag="ACGEXS_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFF8012408.1"
                     /translation="MEERLNRVKQQLQQSSYKLTPQREATVRVLIENEKDHLSAEDVY
                     LKVKDKAPEIGLATVYRTLELLAELKVVDKINFGDGVARFDLRKEGAKHFHHHLVCME
                     CGRVDEIDEDLLPEVENRVENEFNFKILDHRLTFHGVCETCQAKGKG"
     gene            complement(6847..7389)
                     /locus_tag="ACGEXS_001393"
     CDS             complement(6847..7389)
                     /locus_tag="ACGEXS_001393"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFF8012409.1"
                     /translation="MDLNEKTIDRTVIYNGKIVDVEIHTVTLPNGETSTRELVYHNGA
                     VAVCAVTPKKEVVLVKQYRKPVEKPLLEIPAGKLEDDENRVEAAKRELEEETGYIAKE
                     LTHVVDMYGSPGFCDEQLSIYFTDNLEEGTVHLDEDEFVEVIKVPIENVKSMLMNKEI
                     EDAKTIIALQHLLLNYNHSK"
     gene            7471..8379
                     /locus_tag="ACGEXS_001394"
     CDS             7471..8379
                     /locus_tag="ACGEXS_001394"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFF8012410.1"
                     /translation="MQKNILKSGISLSELGLGCMSLGTDLKKAEQIIDCAVENGITYF
                     DTADMYDKGINESVVGKALLKYQQRDDIFIGTKVGNRLTKDGSTTWDPSKSYIKEAVK
                     GSLKRLGIDHIDLYQLHGGTIDDPLDETISAFDELKQEGIIRAYGISSIRPNVIDYYL
                     KHSQIETIMSQFNLIDNRPESLLDAIHNNDVKVLARGPVSKGLLTSNSVNVLDNKFKD
                     GIFDYSHDELGETIASIKEIESNLSALTFSYLTSHDVLGSIIVGASSVDQLKENIENY
                     HTKVSLDQIKTARARVKDLEYTNHLV"
     gene            complement(8595..8843)
                     /locus_tag="ACGEXS_001395"
     CDS             complement(8595..8843)
                     /locus_tag="ACGEXS_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012411.1"
                     /translation="MNCQSESVVRLCVRYAEQLSVFEEFTVLDILGDISVDQISDGTL
                     YYTCEKFKLLVLQGNVLGVQIITNNDESTCEVKYRKMF"
     gene            complement(8859..9614)
                     /locus_tag="ACGEXS_001396"
     CDS             complement(8859..9614)
                     /locus_tag="ACGEXS_001396"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFF8012412.1"
                     /translation="MKGQHFIVTGGTSGLGLSIVRKLLENKVHVTLLVRDVDKATRIF
                     EQELGKTINVIPCDLNDLKSIQALQFEDNTSFDGFIYSAGLGYFKSISDHSFSEMIET
                     YQLNLISFNVLYTVLRPYLTSNAHIVGISSQAAFSTQANAAHYGASKAGFYALMNALR
                     LESPNLHIMTVNVGPIDTPFHQKADPSMKYAKKMGEIMLDANQLAEDIIYGIKTKQLE
                     INRPKWMHHALKMYQIAPRFFERCFPKLFKNKA"
     gene            9756..10571
                     /gene="proC"
                     /locus_tag="ACGEXS_001397"
     CDS             9756..10571
                     /gene="proC"
                     /locus_tag="ACGEXS_001397"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFF8012413.1"
                     /translation="MKLVFYGAGNMAQAIFTGIINSSNLDANDIYLTNKSNEQALKAF
                     AEKLGVNYSYDDATLLKDADYVFLGTKPHDFDALATRIKPHITKDNCFISIMAGIPID
                     YIKQQLECQNPVARIMPNTNAQVGHSVTGISFSNNFDPKSKDEINDLVKAFGSVIEVS
                     EDHLHQVTAITGSGPAFLYHVFEQYVKAGTKLGLEKEQVEESIRNLIIGTSKMIERSD
                     LSMAQLRKNITSKGGTTQAGLDTLSQYDLVSIFEDCLNAAVDRSIELSNIEDQ"
     gene            complement(10676..11596)
                     /gene="rnz"
                     /locus_tag="ACGEXS_001398"
     CDS             complement(10676..11596)
                     /gene="rnz"
                     /locus_tag="ACGEXS_001398"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFF8012414.1"
                     /translation="MEVTFFGTSAGLPTKERNTQAIALNLEPYSNSIWLFDVGEGTQH
                     QILHHAIKLGKVTHIFITHMHGDHIFGLPGLLSSRSFQGGEQKPLTLVGPKGIKAYVE
                     MSMNLSESHLNYPITYIEIDDHLTYHHDGFTVEAHLLNHGIPSYGYRVMAPETTGTIN
                     VEALKNIGLEPGPKYQEVKSHDTFEHNGQVYQSKDFRGESKQGPVVAIFGDTKPCSNE
                     RVISRDADVMVHEATYIDGEKHLANNYHHSHIEDVFALIKEANVKRTLITHLSNRYNT
                     EDINEIYQTLIQNEDTPNFNFVKDFDSFKI"
     gene            11909..13393
                     /gene="zwf"
                     /locus_tag="ACGEXS_001399"
     CDS             11909..13393
                     /gene="zwf"
                     /locus_tag="ACGEXS_001399"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFF8012415.1"
                     /translation="MSTKNKHIPCLITIFGATGDLSHRKLFPSIFHLYQQDNLDEHIA
                     IIGIGRRDITNDDFRNQVKSSIQKHVKDTNKIDAFMEHVFYHRHDVSNEESYQELLDF
                     SNELDSQFELKGNRLFYLAMAPQFFGVISDYLKSSGLTDTKGFKRLVIEKPFGSDLKS
                     AEALNNQIRKSFKEEEIYRIDHYLGKDMVQNIEVLRFANAMFEPLWNNKYISNIQVTS
                     SEILGVEDRGGYYESSGALKDMVQNHMLQMVALLAMEAPISLNSEDIRAEKVKVLKSL
                     RHFQSEDVKKNFVRGQYGEGYIDGKQVKAYRDEDRVADDSNTPTFVSGKLTIDNFRWA
                     GVPFYIRTGKRMKSKTIQVVVEFKEVPMNLYYETDKLLDSNLLVINIQPNEGVSLHLN
                     AKKNTQGIETEPVQLSYSMSAQDKMNTVDAYENLLFDCLKGDATNFTHWEELKSTWKF
                     VDAIQDEWNMVDPEFPNYESGTNGPLESDLLLARDGNHWWDDIQ"
BASE COUNT         4390 a         2425 c         1974 g         4752 t
ORIGIN      
        1 cacatacaaa attccccaca tcaatcaaaa aacgcatgct ataaattaga gtctctaatc
       61 aataacatgc gttctgaaat aactatattc aattttattc tggttttggt agtttaataa
      121 taaatgttgt gcctttccct aattcgcttt taacatctat ggaaccacca tgctcttcga
      181 taatcatttt acaaatgaac aaacctaaac cggtaccttg tttacctcgc gttctcgctg
      241 catcaacttt ataaaaacga tcaaatactt gttgtaaatg ttctggtgca atgcctgtac
      301 ctgtatcttt aatgtataaa atatcttcgc tttcattttc atcacaagta attgcaattt
      361 catctccagg tttcgtataa cgtgatgcat tatcaattaa gttcgttagt acttggtcca
      421 tgcgatccat atcataactc caaacacgct tcttacaata attaaaagtc atatttagac
      481 ctaaatcatc agcttgttgg cgatacttaa ttttcatctt atctagtaac gctgcaatag
      541 gctgaacttc tttatttacg gataaccctt cagtatccat gcgtgcgaca tttaacaatt
      601 cattaactaa acgatttaaa cgtttcgatt catcaaggac aatggcaagc gattctttta
      661 tttcatccgg ttctgtaaca ataccatcta caattgattc agtataacct tgaagtaatg
      721 atatcggtgt acgtaattca tgtgatacat tagcaatgaa atctttcttc atttgatcta
      781 gattgtgctc attagtcata tcacgaactg tcacaacaac accactttta cctccctgtt
      841 caatcttgtc gatatagctt gtggtcacaa caaagaatcg tgcattcatt tctaaatcgc
      901 gcatttcagt ttgttttgat ttaaaagtat cttctatttg tcttaataag aaagctttag
      961 catcttcatc aatattgtcc ataatatcat tcgccatctt attagataag ataatttgtc
     1021 gactctcatt aatacctagg acaccttcta ccatagagtt aattaagctg tctctaatat
     1081 ttttagatgt ggataatgcg tcgacatgct cttcgatttc tgtactcatc tgattaaatg
     1141 cctgcgataa ttgaccaatt tcatctttcg ttgtgacaga aggtttataa gagtaatccc
     1201 cttcagatac acgtgtagct tggtctctta aacgtcttaa aggttttgta attcttgacg
     1261 ataagaaaaa cgcaaagact gttgtaattg ttaagaaaat aacagccgta attatggtga
     1321 taatcgtaat agcattattt gtatcttcga ttgatttcaa gtctttatat ataaagactc
     1381 cactatattt gctatgacta ttcttctgtg cttttgttgg atagcctaac aaaatatatg
     1441 tttgagatga gcccttttct ttaatcgtta catttcgagt aacagattta cctttatcaa
     1501 acacatcgtc aaaatggtcg ttgttgacta cttcattcaa catttgcttt ttaatattag
     1561 aaagtgaagc cgttgattga cgatgtttat tatttataat catcaaccca ccaggatttt
     1621 caattaatgt ttgactatat tttattgctt cttctttatt atgtgattgt tcgaccagtg
     1681 aacttatacg tctagcatct tctcttatgg cattttcggt ttcttgtgtg aaatagtatt
     1741 gcataaaggt aattaaagca atacttaata aaattaaaac tgtcgtcact attaaaataa
     1801 tagttaacca cagtttaatt acgacactat ttagccggct catcattaga tttaacctca
     1861 aatttatacc caacgcccca gactgtttga atcatatgcg cagcttcact agacacacga
     1921 tttaactttt ctctaagtcg tttaacatga gtatcaactg ttcttaaatc accatagaat
     1981 tcataatgcc aaacttcttt taataattgt tcacggtcaa atactttatt tggtgtttta
     2041 gctaaatata ttaataattc gtactcttta ggagtcaaat taacttcttg attatcagca
     2101 agtacgcgat gtgcatcatt atctatttct aaatgtttaa attcaatcac atcacgtgcg
     2161 tgaggttcgc tttgttctac agttgtagat tgcgttcttc ttagaagtgc tttaactctt
     2221 aagactactt ctcttggtga aaatggtttg acgatataat catctgcacc agattcaaaa
     2281 ccttcaacac ggtttgtttc ttcaccttta gcagtcaaca taataatcgg tgtttgttta
     2341 tgttcacgca atttagttgc cacctggata ccatccattt caggcaacat taaatctagt
     2401 agtatgcaag cataattatt ctccattgca agttcataag cctcttggcc attacttgct
     2461 tcatggattt caaaagattc tctttctaaa tacattttaa gtaatcttct gattctatcc
     2521 tcatcatcta cgataagtat ttcgttcgac atacaggtca tacctcccac acatgctttt
     2581 ctttacaaaa gtattatatc acaggaaagc gcatacagtt attaaaaacc taaaattttg
     2641 tgaaaaaatt ctcaaacttt gtgataaata catattttat ttaattattt cccatgttct
     2701 gctaaatgac gcatcacttt tacttcatga ggcgtcaata cacgtccttc accagcattc
     2761 aaaccgacaa catttaaagg cccatattca atacgagaca gtttcgtcac ttgatgacca
     2821 aaatgttcga acattcttct gacttggcga ttacgacctt ctgtaattgt aatttcaacc
     2881 aatgttgtgt ttttatcttt atcttgtttc ttaactttca cttcagccgg ttgcgtcata
     2941 ccatcttcta attcaatacc tttttctagc gctttcactt cttctctcat taaataacct
     3001 tttaatttcg caacatattt tttcttaatt tgatatcttg gatgtgtcat taaattagta
     3061 aattcaccat catttgtgag taataacaat ccagaagtat catagtctaa acgaccaaca
     3121 ggataaatac gtgcttctat atctttaaag taatctgtaa ccactgtacg tcctctatca
     3181 tcagatacac ttgttatcac ttgagttggt ttatggaata aaatgtaaat tttgtcttct
     3241 agttctattt taataccttc aacttcaatc gtatctgatg gcttcacttt tgttcctaat
     3301 tcagtgacag tcgtaccatt cactttcact tttccttcag aaattaaagt ttctgcctta
     3361 cgtcttgaag tataaccgct attagctata cgtttttgta atcgttctaa ttctttagtc
     3421 attattttct cctttttgat tgactagatt actgaaaaaa gcatccattt cttcatcgtc
     3481 ttcttcagtt gtcggcaaat cttctatatt ggaaatacca aatacattta aaaataaatc
     3541 agtagtaatt aactgttggc tacgctgttc attaaccact ttagcctcaa ctagtccttt
     3601 ggcaatcaat gtcttaactg caccatctga attgatacta cgaattaatt caatatcact
     3661 tcttgataat ggctggttat aagcaataat tgatagtact tccattgctg cttgtgataa
     3721 tttcatttgt gacttttgtt caattaattg ttcaatatac gttgccgctt cttttttagt
     3781 cgttaaaaca tacgtcattc caaatcgttg tatcattaat ccatgtgatg aataattttc
     3841 aattaattca acgagttggt ctttcgacat atctaatatt tctaatagtt gtttttcatc
     3901 taaaccttca tcgccagctg taaataaaag cgactctaat ataccatgat tatccaaaat
     3961 ggtagttcac tcctctaata atgttaatat cttcaaaatt acgttgttgc tcaatattaa
     4021 ttattcctgc ttttgacatc tctaaaatag ctaaaaagtg agtgactact tgttcaattg
     4081 gctcagaaaa cgtaaacaga ctaaagaagt taaaatgatc tttatctttc aatctcgatg
     4141 tcacttgttc tgtagcttgt tgaatggtaa atgtctcttt tcggatttca acagatttag
     4201 gtgtatttaa ctcaactcta tttttaactc tttgataagc tacaattaat tcagttaaat
     4261 caatcgtatg atttggatcc caagattcat ctgtttccaa atgagataaa tctgtcggtc
     4321 tttttgtaaa ataaaaatct ctttcttctt tcatgtcatt taaaatagca gtatattctt
     4381 tataattttg atattctatt aaacgcccaa ctaaatcttc ccgtgggtca tcatcaacat
     4441 ccatatctga tgttgattgt ggtaatagca tcttactttt aatcattaag agttctgacg
     4501 ctaatactag gtattcactt gcaatattaa tttcaagctg tttcattgca tgaacgtact
     4561 gcatatactg ctctgttaat gcttgcatag gaatatcata aatatctatt tcaaattttt
     4621 ggataagatg cagcaataaa tctaatggtc cattgaaagc atctaattta acttcataca
     4681 taatatctac ctcgtattgc gtcatatgtg cataattctc taaaatatta tagcatgaaa
     4741 ataacttact tttaaattgg ggtgataaca aaatgcaaca ggcactgata aatttctatt
     4801 atcaatttca tactaagcaa cattatttcc tatgtcatga cattttagaa gatgcatgga
     4861 aagctgaaaa taactacagt aagcaagatg cagttgttag tttaatcttg tttgcaactg
     4921 cttgttacca ttatcgccgc aataatttaa aaggcgccta taagtctttt aataaatcta
     4981 aagaaataat acaaaatgct aaagatagag atgcgttata ccttaattta aatgattatc
     5041 agttgttaat tgagcaacag attgcaaaat tgaatgcagc aaagcctttt tcatctgtta
     5101 tattaccaat aactccagtc tttgaacgca tcatcaaggc caactatcca gactatgatt
     5161 ataatcagga aacagcaaca gatcctttta ttgtagacca tcacatgcgc cgtgatcgtt
     5221 cagaagtgat tgcagcaaaa gaagaagcga ttcaattaag aaaacataga cgtaattaat
     5281 caaccaattt aaaaaagcca caatagcaca gacattttta gtcatgtgta ttgtgacttt
     5341 tgagttattg ttcactttat gctctaggat gaaattggtt atacattttt ctaatttgag
     5401 atttcgaaac atgtgtatag agttgggtag tagatatgtc agagtgacct aacatctctt
     5461 gcactgctct taaatctgcg ccattttcca ataaatgtgt cgcaaaagag tggcgtaacg
     5521 tatgtggcgt taacgtcttt ttaatgtttg cctttacacc attttgttta atcattttcc
     5581 atattgcttg tcgtgataaa ggtttaccat gcatatttaa aaataagact tcagtaacag
     5641 tcttttttaa aagttgcggt ctaatcgttt caatataagt agttaagtac tcaatgactg
     5701 cgtcgcctaa tggtacaatt ctttctttat cgcctttacc aaatacgcgt acaaatccca
     5761 ttattaagtt cacgttttct aactctaaat gtatcaattc agatacacgc attcccgttg
     5821 cgtacagaag ttctaacatc gtacgatcac gatatccatt aattttattt aaatctggcg
     5881 tttctaataa agccaacact tcgtcaacat ttaaaacgtc aggcaatttt ttgtcatatt
     5941 ttggtgaatc taataatacc gttggatctt tcgccgcata tttttctctt atagcaaatt
     6001 gatgaaaact acggattgtt gaaataaatc gcgcaataga tttagcagat tgcccttggt
     6061 ctattaaatg ccccaaacac tcttgaatta attgtcgatc tataaaatca atatgcgaga
     6121 tatgatgttc agtcatataa tcttgatact ttttcaaatc acgtctataa gcaccaattg
     6181 tgttagaact tagtcctttt tcaatttgta taaaacgcaa atattcttca ataattgttt
     6241 ccataaccac acctctaaaa aaagcgaagg ttaatttaac ctacgcaatt tactatcctt
     6301 tacctttagc ttggcacgtt tcacacacac catggaaagt taaacgatga tctaaaattt
     6361 taaaattgaa ctcattttca actcgatttt caacttctgg taacaaatct tcatcgattt
     6421 catctacacg accacattcc atacatacta aatgatggtg gaaatgtttt gcgccttctt
     6481 ttcttaaatc aaaacgagcg acgccatcac caaagttaat tttgtcgaca acttttagtt
     6541 cagctaacaa ctctaacgtt ctgtatactg tcgccaagcc aatttcaggc gctttatctt
     6601 ttactttcag atatacgtct tcagcactta gatgatcttt ttcattttca attagaactc
     6661 taacagtagc ttcgcgttgt ggcgttagct tatatgatga ttgttgtaat tgttgcttaa
     6721 cgcgatttaa tcgttcttcc aacgatgtcc actcccctac taataattaa aatcattaca
     6781 aattatttca aacttcacaa ttaaaactaa cagttttctc aataaaatgc aagcttttct
     6841 catttgttat ttagaatgat tataatttaa taataggtgt tgcaatgcaa tgatagtctt
     6901 cgcatcttct atttctttat tcattagcat cgatttaaca ttctcaatcg gaactttaat
     6961 gacttcgaca aattcatctt catctaagtg aactgtgcct tcttctaaat tatccgtaaa
     7021 atatattgat aattgttcat cgcaaaaacc tggtgaacca tacatatcta caacatgtgt
     7081 taactctttg gcaatatatc ctgtttcttc ttctaattca cgttttgccg cttcgactct
     7141 attttcatca tcttctaatt taccagctgg aatttctagc aatggttttt ctactggttt
     7201 acgatactgt ttcactaata cgacttcttt tttaggtgtc actgcacaaa cagcaactgc
     7261 accattatga taaactaatt ctcttgttga cgtttcaccg tttggtaatg tcactgtatg
     7321 aatttctaca tctacaattt tgccattata aataactgtt cgatcaattg ttttttcatt
     7381 taaatccatt ataatcacgt tcctttaaat tcatattata tattgataca ctatgcttgt
     7441 tagctaaagt gtatcgaaag gagaacagac atgcaaaaaa atatattaaa aagtggtatt
     7501 tcattatctg aattaggtct aggttgcatg agtttaggta cagatttaaa aaaagccgaa
     7561 caaattatag attgtgctgt tgaaaatggt atcacttatt ttgatacagc agatatgtac
     7621 gataaaggta taaatgaatc agttgttggt aaggcacttc ttaaatatca acaacgcgat
     7681 gatattttta ttggtacaaa agtaggcaat cgtttaacaa aagatggcag tacaacatgg
     7741 gatccgagta aatcctatat taaagaggca gttaaaggtt cactaaagcg tttaggtatc
     7801 gatcatatcg atttatatca acttcatggc ggaaccattg atgacccatt agacgaaaca
     7861 ataagcgcat ttgatgaatt gaaacaagaa ggaattatac gtgcttacgg tatttcttct
     7921 attcgcccaa atgtaattga ttattattta aaacatagtc aaatcgaaac gataatgtct
     7981 caattcaatt tgattgataa tcgtccagaa tcattattag atgcaattca caacaatgat
     8041 gttaaagtat tggcaagagg acctgtgtct aaaggattat taacttcaaa cagtgttaat
     8101 gtgctcgaca ataaatttaa agatggtatt tttgattatt ctcatgatga attgggtgaa
     8161 acaatagcct ctattaaaga aattgaaagt aatttatctg cattgacatt tagttattta
     8221 acatcacatg acgtgcttgg ttccatcatt gtaggtgcaa gtagcgtcga ccaattaaaa
     8281 gaaaatattg aaaactatca tactaaagtt agtttagatc agattaaaac agcaagagct
     8341 cgtgtaaagg atttggaata taccaatcat ttagtgtaga agtcattttc agtaataaaa
     8401 acagcagcat gaggcgtttc attataaaaa tgccttactg ctgttgttta tgtacaattc
     8461 gctataattt atgattatga ttactcactt atgatagaaa ttaaagcgtt gtcctcacgc
     8521 atcagtattt agtaatttcg ccttgcggca ttgccttaag caaacttctg ccacttcatc
     8581 tcttaataat tttattaaaa catctttcta tatttcactt cgcatgttga ttcatcatta
     8641 ttagttatta tttgtacacc cagcacattt ccttgcaaca caagtagttt gaatttttca
     8701 caagtataat ataatgtacc gtctgaaatt tggtctacag aaatatcgcc taaaatatcc
     8761 agcactgtaa attcttcaaa tactgatagt tgttccgcat atcgtacaca aagtcttacc
     8821 acactctccg attgacagtt cattgccatc ccacctattt atgctttatt tttaaataat
     8881 ttagggaaac atcgttcaaa aaatctaggc gcaatttgat acattttcaa cgcatgatgc
     8941 atccatttag gccgattaat ttccaattgt tttgttttaa tgccataaat gatatcttct
     9001 gcaagctgat tagcatcaag cataatttcc cccatctttt tagcatactt cattgatggg
     9061 tcggcttttt gatgaaaagg tgtatcaatc gggccaacat taactgtcat gatatgtaag
     9121 tttggtgact ctagtcttaa agcattcatt aatgcataaa accctgcttt cgatgcccca
     9181 taatgtgcag catttgcttg tgtggaaaat gcagcttgac ttgaaatacc tacaatatgt
     9241 gcgttagatg ttaaatatgg tctcaacaca gtatataaaa cattaaaact aattaaatta
     9301 agctgatacg tttcaatcat ttctgaaaaa ctatggtctg aaatagattt gaaataacct
     9361 aaacctgcac tataaatgaa tccatcgaat gatgtattgt cttcaaattg cagtgcctgt
     9421 atcgacttca aatcatttaa gtcacaagga ataacattta tagttttccc caattcctgt
     9481 tcaaagattc tagttgcttt atcaacatca cgcaccaaca acgttacatg cactttattt
     9541 tctagtaact ttcggacaat cgataaacct aaaccactcg taccaccagt cactataaaa
     9601 tgttgtcctt tcatcaatta accttccttt tcaattatat agaatgcaat ttatcaactt
     9661 tacataattg agacaagttg attatctttc ctaatatata tacaataata agaaaatata
     9721 acatacaaat caaaaactaa agggatgtga cgttaatgaa actcgtattt tatggagctg
     9781 gtaatatggc acaagctata tttacaggaa ttattaactc aagcaactta gatgccaatg
     9841 atatatattt aacaaataaa tctaatgaac aagctttaaa agcattcgct gaaaaactag
     9901 gtgttaacta tagttatgat gatgcgacat tattaaaaga tgcagattat gtatttttag
     9961 gtaccaaacc acatgacttt gatgctctag caacacgcat caaaccacat attacaaaag
    10021 acaattgctt catttcaatt atggcaggta ttccgattga ttatattaaa caacaattag
    10081 aatgccaaaa tccagttgct agaattatgc caaacacaaa tgcgcaagtt ggacactctg
    10141 ttactggcat tagtttttca aacaactttg accctaaatc taaagatgaa attaacgatt
    10201 tagttaaagc atttggttct gtaattgaag tatcagaaga tcatttacat caagtaacag
    10261 ctatcaccgg aagcggccca gcatttttat atcatgtatt cgagcaatat gttaaagctg
    10321 gtacgaaact tggtctagaa aaagaacaag ttgaagaatc tatacgcaac cttattatag
    10381 gtacaagtaa gatgattgaa cgttcagatt tgagcatggc tcaattaaga aaaaatatta
    10441 cctctaaagg tggtacgaca caagctggcc ttgatacatt gtcacaatat gatttagtat
    10501 ctattttcga agattgtcta aacgctgccg tcgaccgtag tattgaactt tctaatatag
    10561 aagaccaata aaaacaaacc cgccaacaca tgtatgcatc atcgcaagca ctgtgtttga
    10621 cgggttattt ttataattta ttgttatttg gcaagcattg tttattactt tgtcattaga
    10681 ttttaaaact atcaaaatct tttacaaaat taaaattagg tgtatcttca ttttgtatca
    10741 atgtttgata aatttcattt atatcttctg tattatagcg attgctcaaa tgtgtaatca
    10801 acgtacgttt aacattggct tcttttatca atgcaaatac gtcttcaata tggctatgat
    10861 gataattgtt ggctaaatgc ttttcaccat ctatataggt cgcttcatgt accatcacat
    10921 cagcatctct agaaatcaca cgttcattag aacatggttt tgtatcacca aaaattgcta
    10981 caactggacc ctgtttggac tcacctctaa aatcttttga ttgataaact tgaccattat
    11041 gttcaaatgt atcatgagat tttacttctt gatatttagg acctggttca agaccaatgt
    11101 tttttaacgc ttcaacattg attgtacctg tagtttcagg tgccattact ctatatccat
    11161 atgatggaat accatgatta agtaaatgcg cctctacagt aaaaccatca tgatgatatg
    11221 tcagatgatc atcgatttca atatatgtaa ttggatagtt taaatgtgac tctgataaat
    11281 tcatagacat ttccacatat gctttaattc cttttggtcc aaccaatgta agcggtttct
    11341 gttcaccgcc ctgaaaagaa cgactagaaa gtaatcctgg caaaccaaaa atatgatcgc
    11401 catgcatatg agtaataaat atatgtgtca cttttcctaa tttaattgca tgatgtaaaa
    11461 tttggtgctg tgtaccttca ccaacgtcga aaagccatat ggaattggaa tatggttcta
    11521 aatttaaggc gattgcttgt gtatttctct cttttgtagg caaacctgca ctcgttccaa
    11581 aaaatgtaac ttccatatat gcccctcctt ttcttcaatt cattttatca taaaatttgt
    11641 atcaatttag ttttaattta tacttgaatc aaaatctgtt aaaaattcgt ctttgctagt
    11701 taatttttct aactttaacg tagacataac tatataaatt ttgataatta cgttatactt
    11761 atcattaata agtatcacat taaacatgat acatgaatcg atatttcatt taagacactg
    11821 catacagtcg agcatattgt atgacctact gaatggatta tcttataata ataaatcata
    11881 tatctaatta agaattgagg ttttaatctt gagtactaaa aacaaacaca tcccatgttt
    11941 aatcacaatc tttggtgcaa ctggtgactt aagccatcgt aagttgtttc catcaatatt
    12001 ccatctctac caacaagaca atttagatga acatattgcc atcatcggta ttggacgtcg
    12061 tgacattact aatgatgatt tccgtaatca agtaaaatca tcaattcaaa agcacgtaaa
    12121 agatacaaac aaaattgacg cgtttatgga acatgtcttc tatcatagac atgatgttag
    12181 taatgaagaa agctatcaag aattactaga ttttagtaat gaattagata gccaatttga
    12241 attaaaaggt aatcgactat tctatttagc aatggcacca caattctttg gcgttatttc
    12301 tgattatcta aaatcttctg gtcttactga tacaaaagga tttaaacgcc ttgttatcga
    12361 aaaaccattc ggtagtgatt taaaatcagc cgaagcatta aacaatcaaa ttcgtaaatc
    12421 atttaaagaa gaagaaattt atcgtattga ccactattta ggaaaagaca tggttcaaaa
    12481 tatcgaggta ttacgttttg cgaatgcgat gtttgaacca ttatggaata acaaatatat
    12541 ttcaaacatc caagttacat cttctgaaat actaggtgtt gaagatcgtg gtggttatta
    12601 tgaatcaagt ggcgcgctaa aagatatggt gcaaaaccac atgttacaaa tggttgcatt
    12661 attagctatg gaagcaccta ttagtttaaa tagtgaagat atccgtgctg agaaagtaaa
    12721 agtacttaaa tcactgcgtc atttccaatc tgaagatgtt aaaaagaact ttgttcgtgg
    12781 tcaatatggc gaaggctata tcgatggtaa acaagttaaa gcataccgtg atgaagatcg
    12841 cgttgcagat gactctaaca cacctacctt tgtttcaggt aaattaacaa ttgataactt
    12901 tagatgggct ggtgtaccat tctatattcg tactggtaaa cgtatgaaat ctaaaacaat
    12961 tcaagttgtc gttgaattta aagaagtacc aatgaactta tactatgaaa ctgataaact
    13021 gttagattca aacctattag taatcaatat ccaacctaat gaaggtgtat ctttacatct
    13081 aaatgctaag aaaaatacac aaggtatcga aacagaacct gtccaattgt cttactcaat
    13141 gagcgctcaa gataaaatga atactgtaga tgcatatgaa aatctattat ttgattgtct
    13201 taaaggtgat gccactaact tcacgcactg ggaagaatta aaatcaacat ggaaatttgt
    13261 tgatgcaatt caagatgaat ggaatatggt tgatccagaa ttccctaact atgaatcagg
    13321 tactaatggt ccattagaaa gtgatttact acttgctcgt gatggtaacc attggtggga
    13381 cgatattcaa taattgaatt aaaacgcaca tgttaaacaa aaataaatga gcgaatgaca
    13441 aggattgatt acgaatcaag taaatcaatt gttctatcat tcgctttttt tctagactat
    13501 attatgaaat tatattttac aatgcccaaa actattttaa t
//
LOCUS       DAXWFK010000063        13480 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.063, whole genome shotgun sequence.
ACCESSION   DAXWFK010000063 DAXWFK010000000
VERSION     DAXWFK010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13480
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.063"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(31..924)
                     /locus_tag="ACGEXS_001400"
                     /pseudo
     CDS             complement(31..924)
                     /locus_tag="ACGEXS_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002440612.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            complement(1164..1376)
                     /locus_tag="ACGEXS_001401"
                     /pseudo
     CDS             complement(1164..1376)
                     /locus_tag="ACGEXS_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc peptidase"
     gene            complement(1451..2986)
                     /locus_tag="ACGEXS_001402"
     CDS             complement(1451..2986)
                     /locus_tag="ACGEXS_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFF8012416.1"
                     /translation="MSNIAFYVVSDVHGYIFPTDFTSRNQYQPMGLLLANHVIEQDRR
                     QYDQSFKIDNGDFLQGSPFCNYLIAHSGSSQPLVDFYNRMAFDFGTLGNHEFNYGLPY
                     LKDTLRRLNYPVLCANIYENDSTLTDNGVKYFQVGDQTVGVIGLTTQFIPHWEQPEHI
                     QSLTFHSAFEILQQYLPEMKRHADIIVVCYHGGFEKDLESGTPTEVLTGENEGYAMLE
                     AFSKDIDIFITGHQHRQIAERFKQTAVIQPGTRGTTVGRVVLSTDEYENLSVESCELL
                     PVIDDSTFTIDEDDQHLRKQLEDWLDYEITTLPYDMTINHAFEARVAPHPFTNFMNYA
                     LLEKSDADVACTALFDSASGFKQVVTMRDVINNYPFPNTFKVLAVSGAKLKEAIERSA
                     EYFDVKNDEVSVSADFLEPKPQHFNYDIYGGVSYTIHVGRPKGQRVSNMMIQGHAVDL
                     KQTYTICVNNYRAVGGGQYDMYIDAPVVKDIQVEGAQLLIDFLSNNNLMRIPQVVDFK
                     VEK"
     gene            complement(3037..4581)
                     /locus_tag="ACGEXS_001403"
     CDS             complement(3037..4581)
                     /locus_tag="ACGEXS_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFF8012417.1"
                     /translation="MKKIYKSLTVSAIVATVSLSALPQSLAITHESQPTKQQRTVLFD
                     RSHGQTAGAADWVSDGAFSDYADSIQKQGYDVKAIDGHSNITEASLKSSKIFVIPEAN
                     IPFKESEQAAIVKYVKQGGNVVFISDHYNADRNLNRIDSSEAMNGYRRGAYEDMSKGM
                     NAEEKSSTAMQGVKSSDWLSTNFGVRFRYNALGDLNTSNIVSSKESFGITEGVKSVSM
                     HAGSTLAITNPEKAKGIVYTPEQLPAKSKWSHAVDQGIYNGGGKAEGPYVAISKVGKG
                     KAAFIGDSSLVEDSSPKYVREDNGEKKKTYDGFKEQDNGKLLNNITAWMSKDNDGKSL
                     KASSLTLDTKTKLLDFERPERSTEPEKEPWSQPPSGYKWYDPTTFKAGSYGSEKGADP
                     QPNTPDDHTPPNQNEKVTFDIPQNVSVNEPFEMTIHLKGFEANQTLENLRVGIYKEGG
                     RQIGQFSSKDNDYNPPGYSTLPTVKADENGNVTIKVNAKVLESMEGSKIRLKLGDKTL
                     ITTDFK"
     gene            4810..5766
                     /locus_tag="ACGEXS_001404"
     CDS             4810..5766
                     /locus_tag="ACGEXS_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFF8012418.1"
                     /translation="MKNFKCLFVLMLAVIVFAAACGNSSSLDNQKNASNDSDSKSGGY
                     KPKELTVQFVPSQNAGTLEAKAKPLEKLLSKELGIPVKVSVSTNYNTIVEAMKSKKVD
                     VGFLPPTAYTLAHDQKAADLLLQAQRFGVKEDGSASKELVDSYKSEILVKKDSKIKSL
                     KDLKGKKIALQDVTSTAGYTFPLAMLKNEAGINATKDMKIVNVKGHDQAVISLLNGDV
                     DAAAVFNDARNTVKKDQPNVFKDTRILKLTQAIPNDTISVRPDMDKDFQEKLKKAFID
                     IAKSKEGHKIISEVYSHEGYTETKDSNFDIVREYEKLVKDMK"
     gene            5980..6753
                     /gene="phnC"
                     /locus_tag="ACGEXS_001405"
     CDS             5980..6753
                     /gene="phnC"
                     /locus_tag="ACGEXS_001405"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFF8012419.1"
                     /translation="MSQIEFKNVSKVYPNGHVGLKNINLNIEKGEFAVIVGLSGAGKS
                     TLLRSVNRLHDITSGEIFIQGKSITKAHGKALLEMRRNIGMIFQHFNLVKRSSVLRNV
                     LSGRVGYHPTWKMVLGLFPKEDKIKAMDALERVNILDKYNQRSDELSGGQQQRISIAR
                     ALCQESEIILADEPVASLDPLTTKQVMDDLRKINQELGITILINLHFVDLAKEYGTRI
                     IGLRDGEVVYDGPASEATDDVFSEIYGRTIKEDEKLGVN"
     gene            6755..7555
                     /gene="phnE"
                     /locus_tag="ACGEXS_001406"
     CDS             6755..7555
                     /gene="phnE"
                     /locus_tag="ACGEXS_001406"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFF8012420.1"
                     /translation="MPLEIPTKYDSLLKKKVSLKTSFTFMLIIVLIIWSFIYTGFNFG
                     DLMIGIPQIGDLFKQMIPPDFEYLQQITTPMLDTIRMAIVSTVLGSIVSIPIALLCAS
                     NIVHQKWISIPSRFILNIVRTIPDLLLAAIFVAVFGIGQIPGILALFILTICIIGKLL
                     YESLETIDPGPMEAMTAVGANKIKWIVFGVVPQAISSFMSYVLYAFEVNIRASAVLGL
                     VGAGGIGLFYDQTLGLFQYPKTATIILFTLVIVVVIDYISTKVRAHLA"
     gene            7552..8367
                     /gene="phnE"
                     /locus_tag="ACGEXS_001407"
     CDS             7552..8367
                     /gene="phnE"
                     /locus_tag="ACGEXS_001407"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFF8012421.1"
                     /translation="MTQEIAKYNVHTKAHKRKLIKRWLIAIVVLAIIIWAFAGVPSLE
                     LKSKSLEILKSIFSGLFHPDISYIYIPDGEDLLRGLLETFAIAVVGTFIAAIICIPLA
                     FLGANNMVKLRPVSGVSKFILSVIRVFPEIVMALIFIKAVGPGSFSGVLALGIHSVGM
                     LGKLLAEDIEGLDFSAVESLKASGANKIKTLVFAVIPQIMPAFLSLILYRFELNLRSA
                     SILGLIGAGGIGTPLIFAIQTRSWDRVGIILIGLVLMVAIVDLISGSIRKRIV"
     gene            complement(8498..9676)
                     /gene="deoB"
                     /locus_tag="ACGEXS_001408"
     CDS             complement(8498..9676)
                     /gene="deoB"
                     /locus_tag="ACGEXS_001408"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFF8012422.1"
                     /translation="MTRPFNRVHLIVMDSVGIGEAPDAADFKDEGSHTLRHTLEGFDQ
                     TLPNLEKLGLGNIDKLPVVNAVEQPEAYYTKLSEASVGKDTMTGHWEIMGLNIMQPFK
                     VYPNGFPEELIQQIEEMTGRKVVANKPASGTQIIDEWGEHQMKTGDLIVYTSADPVLQ
                     IAAHEDIIPLEELYDICEKVRELTKDPKYLIGRIIARPYVGEPGNFTRTSNRHDYALK
                     PFGKTVLDHLKDGGYDVIAIGKINDIYDGEGVTEAVRTKSNMDGMDQLMKIVKKDFTG
                     ISFLNLVDFDALYGHRRDKPGYAQAIKDFDDRLPELFSNLKEDDLVIITADHGNDPTA
                     PGTDHTREYIPVIMYSPKFKGGHALESDTTFSSIGATIADNFNVTLPEFGKSYLKELK
                     "
     gene            complement(9704..10366)
                     /gene="deoC"
                     /locus_tag="ACGEXS_001409"
     CDS             complement(9704..10366)
                     /gene="deoC"
                     /locus_tag="ACGEXS_001409"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFF8012423.1"
                     /translation="MKFEKYIDHTLLKPESTRTQIDQIIDEAKAYNFKSVCVNPTHVK
                     YAAERLADSEVLVCTVIGFPLGASTTATKVFETEDAIQNGADEIDMVINIGALKDGRF
                     DDVQQDIEAVVKAAKGHTVKVIIETVLLDHDEIVKASELTKAAGADFVKTSTGFAGGG
                     ATAEDVKLMKDTVGADVEVKASGGVRNLEDFNKMVEAGATRIGASAGVQIMQGLEADS
                     DY"
     gene            complement(10447..11799)
                     /gene="tet(38)"
                     /locus_tag="ACGEXS_001410"
     CDS             complement(10447..11799)
                     /gene="tet(38)"
                     /locus_tag="ACGEXS_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(38)"
                     /protein_id="HFF8012424.1"
                     /translation="MNVEYSKIKKAVPILLFLFVFSLVIDNSFKLISVAIADDLNISV
                     TTVSWQATLAGLVIGIGAVVYASLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFPLL
                     LVGRIIQTAGLAAAETLYVIYVAKYLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFIST
                     YLHWTNMFLIALIVVFTLPFLFKLLPKENNTNKAHLDFVGLILVATIATTVMLFITNF
                     NWLYMIGALIAIIVFALYIKNAQRPLVNKSFFQNKRYASFLFIVFVMYAIQLGYIFTF
                     PFIMEQIYHLQLDTTSLLLVPGYIVAVIVGALSGKIGEYLNSKQAIITAIILIALSLI
                     LPAFAVGNHISIFVISMIFFAGSFALMYAPLLNEAIKTIDLNMTGVAIGFYNLIINVA
                     VSVGIAIAAALIDFKALNFPGNDALSSHFGIILIILGLMSIVGLVLFVSLNRWTQSEK
                     "
     gene            complement(11806..12513)
                     /gene="deoD"
                     /locus_tag="ACGEXS_001411"
     CDS             complement(11806..12513)
                     /gene="deoD"
                     /locus_tag="ACGEXS_001411"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFF8012425.1"
                     /translation="MKSTPHIKPMNDVEIAETVLLPGDPLRAKFIAETYLDDVEQFNT
                     VRNMFGFTGTYKGKKVSVMGSGMGMPSIGIYSYELIHTFGCKKLIRVGSCGAMQENID
                     LYDVIIAQGASTDSNYVQQYQLPGHFAPIASYQLLEKAVETARDKGVRHHVGNVLSSD
                     IFYNADTTASERWTRMGILGVEMESAALYMNAIYAGVEALGVFTVSDHLIHETSTTPE
                     ERERAFTDMIEIALSLV"
     gene            12749..>13480
                     /locus_tag="ACGEXS_001412"
     CDS             12749..>13480
                     /locus_tag="ACGEXS_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8012426.1"
                     /translation="MEVVYVTQTQPKFLTIYNTLYKEIQIGKYPSGHALPTEKELCAR
                     FDVSRMTLRQAIKLLAEDGIVESTRGKGHFVIPQTNAQHASSMDQFKHPLDQILLARV
                     SMSSINYRVDLESEYTNHLFANHPSAVIAMERYYQKKDNHSKQADAFCFTFIPLNVID
                     TFKVNTQNEDDMKTFVEQTIYSNAYQSDLKMSITKAPHFKNHSHVFDGDTHCWLIIET
                     LYAQTPYPIMINKWYIPQEISELTLT"
BASE COUNT         4404 a         2489 c         2103 g         4484 t
ORIGIN      
        1 taagtgaaca tagttcttta gttataataa ttaattcaac caaaagtcga tttgtttttg
       61 caattggttt tcatttcctc ttaaagatat tttcattaaa tctgtcaaat caatagacgc
      121 tatatttttc aacttatctc tatatttatt tttagtacgt ctttctaaat ttccccattc
      181 ctcttcttcg tgagttaata aatgaagcat tgctcgttct tgtatatttt caatcatttt
      241 taaattcggt tttaaaatat gcaaatcatc aaaacaatct ttccaacaat caaccatatc
      301 tcgttttaat tcaatttcca cacgccatag aaatgttgaa tcaatttcaa catctgcatt
      361 atctttacgt tcttgttttt attataaatc cgaataaacc tatcactatt acgcacacca
      421 aaatattttg tttctggttt tacattacgt ccataaaata tagttttctt taccgactta
      481 tctgacaatg cataatagtc atttaaatca aattcaaaat caaaagccaa atctaatctc
      541 gtaaaactaa catcgtccaa ataactgatg atattttgtt ttaaccaaag cacttcatca
      601 tgcgaaagct tattaggatt aaattcaacg cgcatactac gtctattcca aagagttgct
      661 tttattttgt catattcaat ataaactttt tctttaagag ctttagcttt aaagtttgtt
      721 tgtaaaatat cccaaagccg aatttcagga ttagtactca taaaatgtga aagtctctct
      781 gcgttagaca tgctaagatt cccaacaatc gttatagcgt caaaagacaa ttttggaata
      841 gctagtgaca tcctatgtcg atttaaccgg ctattaccgg atattagagt atccagtttt
      901 acaaatggat gaaacgaaat tcaaaacact aaaaaatatg ttccactaac agcaaaaaaa
      961 taccattatg ttcctactaa aaaactaaaa atactggaga acaaatgtca ggatataact
     1021 taggatacta tgtaataaaa atttacaata aaaaaacagg aaaacaaatt tcaagtaaaa
     1081 gaatacccat acaaagagga taaaataaaa aacctcgaac tgaaatgatg atcttttcag
     1141 ctcgaggttt aaatattggt gccttattta tatagattcg ttatattata ttctctattt
     1201 tcattaacat aatccttaaa gagttttaaa ttaatacctg ctagatgatt caaaaatgtt
     1261 tcatcaactt ttaaataatt caataatttt tgtggtgtca gtaaatttct atcaaaatac
     1321 aactttaata aactattcat tttgacagga cgtgacattt caatcacgtc gtctaaagat
     1381 aatactttct cgctttaaac aaaaacaaaa acttacccga ttaaaatcaa gtaagtttta
     1441 catatatccg tcacttttca actttaaaat caacaacttg cgggatgcgc atcaaattat
     1501 tatttgataa aaaatcaata agtaattgtg cgccttcaac ttgaatatct tttacaactg
     1561 gcgcgtcgat atacatatca tactgaccac cgcctactgc acgataatta tttacacaaa
     1621 ttgtatatgt ctgctttaaa tcaactgcgt gaccttgaat catcatattg ctcacacgtt
     1681 gtccctttgg tcttccaaca tgaatggtat aacttacgcc accatatata tcataattaa
     1741 agtgttgtgg tttgggttca aggaagtctg cgctcacact aacttcatca tttttcacgt
     1801 caaaatattc tgctgatcgt tcaatggctt ctttaagttt ggcaccactt acagctaaaa
     1861 ctttaaatgt atttggaaat gggtaattgt taataacatc tcgcatcgtc acgacttgct
     1921 tgaaaccact agcagaatca aacaaagctg tacaggcaac atctgcgtca cttttttcta
     1981 ataaagcgta attcataaaa tttgtaaaag gatgcggtgc cacacgtgcc tcaaatgcat
     2041 gattaatcgt catatcatat ggcaatgtag taatttcgta atctaaccag tcctctaact
     2101 gctttcgtaa atgttggtca tcttcatcaa tagtaaatgt ggaatcatct ataacaggaa
     2161 gtaattcaca tgattcaacg gatagatttt catattcatc agtactcaag actactctgc
     2221 ctacagttgt acctctcgta ccaggttgaa tcacagccgt ttgcttaaac ctttcagcaa
     2281 tttgtcgatg ttggtgaccc gtaataaaga tatctatatc tttagaaaac gcttctaaca
     2341 tggcatatcc ttcattttca cccgttaata cttcggtcgg cgtaccactt tctaaatcct
     2401 tttcaaatcc accatggtaa caaaccacaa tgatatctgc atgtcgcttc atttcaggta
     2461 agtattgttg aagtatttca aaagcactat gaaacgtaag tgactgaata tgctctggtt
     2521 gttcccaatg gggaataaat tgtgtcgtta aacctatcac accaacagtt tgatctccaa
     2581 cctgaaaata cttcacaccg ttatcagtca atgtactatc attttcatat atattagcgc
     2641 acaaaactgg ataattgagt ctgcgtaaag tgtcttttaa gtatggtaat ccataattaa
     2701 attcatgatt accaagcgta ccaaagtcga atgccattcg attataaaaa tcaactaaag
     2761 gctggctact gccgctatgc gcgattaagt aattacaaaa tggtgaccct tgcaaaaaat
     2821 caccattatc tattttaaaa ctttggtcat actgccttct gtcttgttct ataacatgat
     2881 tcgctagtaa caatcccata ggttgatatt gatttctact cgtaaaatct gttgggaaaa
     2941 tataaccatg tacgtcactc acgacataaa atgctatgtt tgacatcctc actcactcct
     3001 tcaatcacaa acatctttct tatttctatt atatatttat ttgaagtctg ttgtaatcaa
     3061 ggttttgtca ccgagtttta aacgaatctt tgaaccttcc atactttcaa gtactttagc
     3121 attgacctta attgtgacat ttccgttttc atctgcttta actgttggca aagtactgta
     3181 acctggtggg ttataatcgt tatctttact tgaaaattgt ccgatttgac gtccgccttc
     3241 tttgtaaata ccaactctaa gattttcaag tgtttgattt gcttcaaatc cttttaaatg
     3301 tattgtcatt tcaaatggct catttacaga aacattttgc gggatatcaa atgttacttt
     3361 ttcgttctga tttggtggtg tatgatcatc tggtgtgttt ggctgaggat ctgcgccttt
     3421 ttcgctgcca taactacctg ctttaaatgt tgttggatca taccatttat aaccactcgg
     3481 cggttgtgac catggctctt tttcaggctc agttgaacgc tctggtcgtt caaaatcaag
     3541 caacttagtc tttgtatcta atgttaggct actcgcctta agtgatttcc catcattatc
     3601 tttagacatc caagccgtta tattatttaa tagcttaccg ttgtcttgtt ctttaaaacc
     3661 atcatatgtt ttcttctttt ctccattatc ttctcttaca tatttgggcg aactatcttc
     3721 cacaagtgat gaatcaccga taaatgctgc tttacctttt ccaactttag aaattgctac
     3781 ataggggcct tctgctttac cgcccccatt ataaatacct tgatctacag catgtgacca
     3841 tttacttttc gctggcaatt gttctggtgt atacacaata ccttttgctt tctctggatt
     3901 agtaattgct aatgtcgatc cggcatgcat agagacagat ttcacacctt cagtaatacc
     3961 gaaactttct tttgaagaaa caatattgct cgtatttaaa tcacctagtg cattatatcg
     4021 aaaacgtacg ccaaagtttg tagataacca atctgaactt ttcacacctt gcattgcagt
     4081 agaacttttt tcttctgcat tcataccttt cgacatatct tcatatgctc cacgtcgata
     4141 accattcatt gcctccgatg aatcaatacg atttaaattt cggtcagcat tgtaatgatc
     4201 tgaaataaag acaacattgc caccttgttt cacatattta acaattgctg cctgttctga
     4261 ttctttgaaa ggaatgttag cctcaggaat tacaaatatt ttggaacttt tcaaacttgc
     4321 ttctgttatg ttcgaatgac catcaatagc tttaacgtca taaccttgtt tttgtattga
     4381 atccgcataa tctgaaaatg caccatcact aacccaatct gcagcaccag ctgtttgacc
     4441 atgagaacga tcgaataata ccgttcgctg ttgctttgta ggttgcgatt catgcgttat
     4501 agctaaagat tgcggtaaag cacttaatga taccgttgca acaattgcag agacagttaa
     4561 tgacttatat atttttttca ttttgtgagg ctccttttaa aataaatttg ttcttgaatt
     4621 ataggataaa aattcgttgc atatgagcaa tttaacgaaa aatttacaaa atcttatcaa
     4681 actcttaaag aaagttatta aaattcattt ttataaaata ctttttaaca tttaaatgtg
     4741 gtacgctata agtgtaattt cattgcatac atattacacg attaagaatg tgaaggggac
     4801 agttatcaaa tgaaaaattt taagtgttta tttgtattaa tgttagcagt cattgttttt
     4861 gcagcagcat gtggaaactc aagttcttta gataatcaaa agaacgctag taatgattcg
     4921 gattctaaat caggaggata caaacctaaa gaattaaccg ttcaatttgt accttcgcaa
     4981 aatgctggaa cattagaagc taaagcaaaa ccattagaaa aattactatc taaagaatta
     5041 gggattccag ttaaagtgtc tgtatcaact aactacaata caattgttga agctatgaag
     5101 tctaaaaaag ttgatgttgg tttcttacca ccaacggcat acacattagc acatgatcaa
     5161 aaagcagctg atttattatt acaagcacaa cgtttcggtg taaaagaaga tggttcagca
     5221 agtaaagaac ttgtagatag ttataaatca gaaattcttg ttaaaaaaga ctcaaaaatt
     5281 aaaagcttga aagatttaaa aggtaagaaa attgccttac aagatgtaac atcaactgct
     5341 ggatatacat tcccacttgc gatgttaaaa aacgaagcag gtattaatgc aactaaagat
     5401 atgaaaattg tgaatgttaa aggtcatgac caagcagtta tctcattatt aaatggagat
     5461 gtagatgctg cggctgtatt taacgatgca cgtaatactg tgaaaaaaga ccaaccaaat
     5521 gtatttaaag acacacgaat tttaaaatta acacaagcta ttccgaatga cacaatttct
     5581 gtaagaccag atatggataa agattttcaa gaaaaattga aaaaagcttt tatagacatt
     5641 gctaaatcaa aagaaggtca caaaattatt agcgaagttt attcacatga aggatacaca
     5701 gaaacgaaag attcaaattt cgacattgta agagagtacg aaaaattagt taaagatatg
     5761 aaataatcat tatttaacaa atgaatcatt agcgaatttg gtattaaaag ctttcgttca
     5821 atagatatat tctagattaa tattgaaaag ctaggcgcta aactgaaaca gatatagaaa
     5881 ggtgtcgctg tacatttgaa accatttgta cacagaaacc caatgtctat gatatttcag
     5941 tttaccttgg cttttcttta ttaaagaaag gtgtcaaaca tgagtcaaat cgaatttaaa
     6001 aacgtcagta aagtctatcc taacggtcat gtaggcttga aaaatattaa cttaaatatt
     6061 gaaaaaggtg aatttgcagt tattgtcgga ctatctggtg ctgggaaatc cacgttatta
     6121 agatctgtaa atcgtttgca tgatatcacg tcaggtgaaa ttttcatcca aggtaaatca
     6181 atcactaaag cccatggtaa agcattatta gaaatgcgcc gaaatatagg tatgattttc
     6241 caacatttta atttagttaa acggtcaagt gtattacgaa atgtactaag tggacgtgta
     6301 ggttatcacc ctacttggaa aatggtatta ggtttattcc caaaagaaga caaaattaag
     6361 gcaatggatg cactagaacg cgtcaatatc ttagataaat ataatcaacg ctctgatgaa
     6421 ttatcaggtg gccaacaaca acgtatatct attgcacgtg cgctatgcca agaatctgaa
     6481 attattcttg cagatgaacc agttgcttca ttagacccat taactacgaa acaggttatg
     6541 gatgatttaa gaaaaatcaa ccaagaatta ggcatcacaa ttttaattaa tttacatttt
     6601 gttgacttgg caaaagaata tggcacacgc atcattggtt tacgtgatgg tgaagttgtc
     6661 tatgatggtc ctgcatctga agcaacagat gacgtattta gtgaaatata tggacgtaca
     6721 attaaagaag atgaaaagct aggagtgaac taacatgcct ttagaaatac ctacaaagta
     6781 tgactccctt ttaaagaaaa aggtttcttt aaaaacgagt tttaccttca tgttaatcat
     6841 tgtgcttatc atttggagct tcatttatac gggatttaat tttggagatt taatgatagg
     6901 aatacctcaa ataggtgatc tattcaaaca aatgattcca cctgatttcg agtatttaca
     6961 acaaattaca acgccaatgt tagataccat tcgaatggct atcgtaagta cagtattagg
     7021 tagcatcgtt tcaataccaa ttgcgttatt atgtgctagc aatatcgttc atcaaaagtg
     7081 gatttcaata ccctcgcgct ttattttaaa tatagttcgt actattccag atttgttatt
     7141 agcagcaatc tttgtggctg tatttggaat cggtcaaatt ccagggatat tagcactgtt
     7201 tattttaact atctgtatta ttggaaaatt attatatgaa tcattggaaa cgatagatcc
     7261 aggtccaatg gaagcaatga cggctgttgg cgctaataaa ataaaatgga ttgttttcgg
     7321 tgttgtacca caagccatat cgtcatttat gtcatacgta ttatatgcat ttgaagtaaa
     7381 tatacgtgct tcagctgtgc ttggattagt cggcgctggc ggtattggat tgttttatga
     7441 tcaaacactt ggtttatttc aatatccaaa aacagcaacg attattttat ttactttagt
     7501 tatcgtcgtc gtcattgatt acatcagtac gaaagtgagg gcacatctcg catgacacag
     7561 gaaatagcaa aatataatgt tcacacaaaa gcacacaaac gaaaattgat taaaagatgg
     7621 cttattgcaa ttgtcgtctt agctattatc atctgggcat ttgcaggtgt accaagttta
     7681 gaacttaaaa gtaaatcatt agaaatctta aaatccatat tcagcggatt attccatcct
     7741 gatatcagct atatctatat accagatggc gaagacttat tacgtggttt acttgaaacc
     7801 tttgcgatag ccgttgtagg tactttcatc gccgcaatta tctgtattcc attagcattt
     7861 ctaggtgcaa ataatatggt aaagctacgc ccagtttcag gtgttagcaa atttatttta
     7921 agtgttatac gtgtcttccc agaaattgta atggcactta tatttatcaa agctgttggc
     7981 ccaggttcat tttcaggtgt attagcttta ggtatccatt ccgtaggtat gcttgggaaa
     8041 cttttagctg aagatattga aggtctagat ttcagtgctg tagaatcatt aaaggccagt
     8101 ggtgcgaata agattaaaac actcgtattt gcagtcatac cacaaattat gcctgccttt
     8161 ctatcactca tactttatcg ctttgaacta aacttacgtt cagcttctat actggggcta
     8221 attggggctg gtggtatcgg gacaccactc atatttgcca ttcaaacacg ttcttgggac
     8281 cgtgtaggta ttatattaat cggtttagta ctaatggtcg caattgtcga tttaatttcc
     8341 ggttcaatcc gaaaacgtat tgtttaacat taaatcagga tactcctaaa taagaagtcc
     8401 taccgtctta cgtttctcta ttataataaa aacagcagtg aagaaaacta ttgttatagt
     8461 taacttcact gctgttttta taatatctaa atttattcta tttcaattcc tttaaataac
     8521 ttttaccgaa ctctggtaat gttacgttga aattatctgc tatagttgca ccgatagaac
     8581 tgaatgtagt atcactttct agtgcatgac cacctttaaa tttcggactg tacataatta
     8641 ctgggatata ttctctcgta tggtccgtac ctggcgctgt cgggtcatta ccatggtctg
     8701 ctgtaataat tactaaatcg tcttctttta agttgctaaa cagttctggc aagcgatcat
     8761 cgaaatcttt aattgcttgt gcataacctg gtttatcacg acgatgaccg tataatgcat
     8821 caaagtctac taagtttaag aagctaatac ctgtgaaatc tttcttaaca attttcatca
     8881 attgatccat accgtccatg ttactcttcg tacgaaccgc ttctgttaca ccttcaccat
     8941 cataaatgtc attaatttta ccgatggcaa taacatcata accaccgtct ttcaaatgat
     9001 ctaagacagt tttaccaaaa ggttttaacg catagtcatg tcgattagat gtacgtgtaa
     9061 agtttcctgg ttcaccaaca tatggacgtg cgataatacg accaattaaa tatttagggt
     9121 cttttgtcaa ctcacgaacc ttttcacaaa tatcatataa ctcttctaat gggataatgt
     9181 cttcatgtgc agcaatttgc aatactgggt ctgcacttgt ataaacaatt aagtcaccag
     9241 ttttcatttg gtgctcgccc cactcatcga taatttgcgt acccgatgcc ggtttgttag
     9301 caacaacttt acgacctgtc atttcttcaa tttgttgaat taactcttca gggaatccat
     9361 tagggtatac tttaaaaggt tgcataatat ttaatcccat aatttcccag tgaccagtca
     9421 ttgtatcttt accaactgaa gcttcactca atttagtata gtatgcttct ggttgttcaa
     9481 ctgcatttac tactggtaat ttatcgatgt tccctagacc taacttttca aggtttggta
     9541 aagtttgatc gaaaccttct aaggtatgtc ttaaagtatg tgaaccttca tctttaaaat
     9601 cagctgcgtc tggcgcttca ccaataccta ctgaatccat tacgattaaa tgtacacgat
     9661 taaatggtct tgtcatagct atcactccca aaatttatat atattagtaa tctgaatctg
     9721 cttctaaacc ttgcataatt tgaacacctg cgctcgcacc aatacgtgtc gcacctgctt
     9781 caaccatttt attgaaatct tctaaattac gtacgccacc tgatgctttt acttctacat
     9841 cagcacctac tgtatctttc attaatttaa cgtcttctgc agtcgcaccg ccacctgcaa
     9901 aacctgttga agttttaacg aagtccgcac cagccgcttt tgttaattca ctcgctttta
     9961 caatttcgtc atggtccaac aataccgtct caataatcac ttttactgtg tgacctttcg
    10021 cagctttaac cactgcttca atgtcttgtt gtacatcatc aaaacgtcca tcttttaatg
    10081 cgccgatgtt gatgaccatg tcaatttcat ctgcaccatt ttgaattgca tcttctgttt
    10141 caaatacttt cgttgcagtt gtcgacgcac ctaatgggaa tcctattacc gtacaaacga
    10201 gcacctctga atcagctagt cgctctgctg catatttaac atgtgttgga ttcacacata
    10261 cagatttaaa attgtatgct ttcgcttcat cgatgatttg atcgatttgc gtacgtgttg
    10321 actcaggctt caataaagtg tgatctatat atttctcaaa tttcatactt actactcctc
    10381 gtgttatata atctctttat ttaattttac tataaatacg aatatatctc gcgaatttat
    10441 atctatttat ttttcagatt gtgtccaacg atttaagctg acgaataaaa ctaatccgac
    10501 aatactcatt aaacctaaaa taattaaaat aataccgaaa tgtgaactta atgcatcatt
    10561 gcctgggaaa tttaatgctt taaaatcgat tagagccgca gcaatcgcaa tacctacaga
    10621 taccgccaca ttaataatta aattataaaa accaatagcc acacctgtca tattaagatc
    10681 tattgtttta atggcttcgt taagtaaagg tgcatacatt aaagcaaagc tacctgcaaa
    10741 gaatatcata gaaatgacga agattgaaat gtgattacct actgcaaatg caggtaaaat
    10801 caagctcagt gctattaaaa taattgctgt gataatcgct tgttttgaat tcagatattc
    10861 gccgatttta ccacttagtg caccaacaat gactgctact atataacccg gtactaataa
    10921 cagtgatgtt gtgtctagtt gcagatgata aatttgctcc attatgaatg ggaacgtaaa
    10981 aatataaccc aattggatag catacattac aaatactata aataaaaatg aagcataacg
    11041 tttattttgg aaaaatgatt tatttactaa tggacgttgc gcatttttaa tatatagcgc
    11101 aaaaacgata atcgcaatta aggcaccaat catatataac caattaaagt tcgtaataaa
    11161 cagcatgact gttgtagcaa tagttgccac tagaattaag ccaacaaaat ctaaatgagc
    11221 tttattcgta ttattttctt ttggtaataa tttaaatagg aatggcaacg taaatactac
    11281 gattaatgca attaaaaaca tatttgtcca gtgtaaatac gtagaaataa atccacctga
    11341 taatgtaccg ataactaatg acaaggaata actgctcgta cttaagccaa ggtaagtctt
    11401 ctggtcctct ttagaaagat actttgcaac atatatcaca tataatgtct ctgcagcagc
    11461 taaaccggca gtttgaataa tacgtccaac taaaagtaat gggaattgat gttggaaaat
    11521 gtaaccaata attgatccga taatgattaa tatcacacca taaataaata gtgtgcgtat
    11581 actaatggca tcagataatg aagcgtatac tacagcgcca ataccaatta ctaaaccggc
    11641 taatgtcgct tgccaactta ctgtcgttac agatatgttt aagtcatcag caatggctac
    11701 agaaatcaat ttaaatgagt tgtctataac caaactgaat acaaataaga ataataaaat
    11761 aggtactgct ttctttattt tagaatattc aacattcata atcatctaca ccaatgacag
    11821 tgcaatttca atcatatctg taaatgcacg ttccctttcc tcaggtgttg ttgacgtttc
    11881 atgaattaaa tgatcgctca ctgtgaacac acctaatgct tcgacaccag cgtaaattgc
    11941 attcatgtat aatgcagctg attccatttc tacacctaaa atacccatac gcgtccaacg
    12001 ttcactcgct gttgtatccg cgttatagaa aatatcactt gataacacat tacctacatg
    12061 atgacgtaca cctttgtcac gtgctgtttc aactgctttt tctaataatt gataagaagc
    12121 aattggcgca aaatgacctg gtaattgata ttgttgaacg taatttgaat cagtagaggc
    12181 accttgtgca ataatcacat catataaatc aatgttttct tgcatcgcgc cacaagagcc
    12241 aacgcgaatt aattttttac aaccaaatgt atgaattaat tcataagagt aaatgccaat
    12301 agatggcata cccatacctg aacccatgac agaaactttt ttacctttat atgttccggt
    12361 aaaaccaaac atgtttcgca ctgtattgaa ctgttccaca tcatccaaat aagtttctgc
    12421 aatgaactta gctcttaacg gatctcctgg caatagaacc gtttctgcaa tttcgacgtc
    12481 attcattggt ttaatgtgtg gtgttgattt cataaaatat tcctcccaat atttgtatag
    12541 tgatttgtct atttgtctaa ccatgtatat aataatattt tttaaaagcg ctttcaagca
    12601 ttacattttg acaatattaa gaacatttta cagttacagc tactaacctt gtataaaact
    12661 aattttcaaa gcctatctat atttaatttt tataattttt ccaatttgag taaaaattat
    12721 tgtatgatga gagtgattaa gtaactttat ggaggttgtc tacgtgacgc aaacacaacc
    12781 aaaattttta acgatttata atacgttata taaagaaatt caaataggaa aatatccgag
    12841 tggccatgca ttacctactg aaaaggaact ttgtgcgcgc tttgatgtca gtcgtatgac
    12901 tttgcgccaa gctataaaat tgttagccga agatggcatc gtagagagta caagagggaa
    12961 gggacacttt gttattccac aaacaaatgc ccaacacgct tcaagtatgg atcaatttaa
    13021 gcatccactt gatcaaatat tgctagctcg cgtatctatg tcatcaatta attatcgtgt
    13081 tgatttagaa agtgaatata caaatcattt gtttgcaaac catccttctg ccgtcattgc
    13141 aatggaacgc tattatcaaa aaaaggacaa tcattctaag caagctgatg cattttgctt
    13201 tacctttata ccgttaaatg tcattgacac atttaaagtg aatacacaaa acgaagacga
    13261 catgaagaca ttcgttgagc aaacgattta tagcaatgca tatcaatcag atttaaaaat
    13321 gtctattacc aaagcgcccc acttcaaaaa tcatagtcac gtgtttgatg gtgatacaca
    13381 ttgttggctg attattgaaa cactgtacgc acaaacacct tatccaatta tgattaataa
    13441 gtggtatatt ccacaggaaa tatctgaatt aacactgaca
//
LOCUS       DAXWFK010000064        13432 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.064, whole genome shotgun sequence.
ACCESSION   DAXWFK010000064 DAXWFK010000000
VERSION     DAXWFK010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13432
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.064"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(138..698)
                     /locus_tag="ACGEXS_001413"
     CDS             complement(138..698)
                     /locus_tag="ACGEXS_001413"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFF8012427.1"
                     /translation="MSRKTALLVLDMQEGIASSVPRIKNIIKANQRAIEAARQHRIPV
                     IFIRLVLDKHFNDVSSSNKVFSTIKAQGYAITEADASTRILEDLAPLEDEPIISKRRF
                     SAFTGSYLEVYLRANDINHLVLTGVSTSGAVLSTALESVDKDYYITVLEDAVGDRSDD
                     KHDFIIEQILSRSCDIESVESWKSSL"
     gene            complement(866..2773)
                     /gene="sasF"
                     /locus_tag="ACGEXS_001414"
     CDS             complement(866..2773)
                     /gene="sasF"
                     /locus_tag="ACGEXS_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasF"
                     /protein_id="HFF8012428.1"
                     /translation="MAKYRGKPFQLYVKLSCSTMMATSIILTNILPYDAQAASEKDTE
                     ITKEILSKQDLLDKVDKAIRQIEQLKQLSASSKEHYKAQLNEAKTASQIDEIIKRANE
                     LDSKDNKSSHTEMNGQSDIDSKLDQLLKDLNEVSSNVDRGQQSGEDDLNAMKNDMSQT
                     ATTKHGEKDDKNDEAMVNKALEDLDHLNQQIHKSKDASKDTSEDPAVSTTDNNHEVAK
                     TPNNDGSGHVVLNKFLSNEENQSHSNRLTDKLQGSDKINHAMIEKLAKSNASTQHYTY
                     HKLNTLQSLDQRIANTQLPKNQKSDLMSEVNKTKERIKSQRNIILEELARTDDKKYAT
                     QSILESIFNKDEAVKILKDIRVDGKTDQQIADQITRHIDQLSLTTSDDLLTSLIDQSQ
                     DKSLLISQILQTKLGKAEADKLAKDWTNKGLSNRQIVDQLKKHFASTGDTSSDDILKA
                     ILNNAKDKKQAIETILATRIERQKAKLLADLITKIETDQNKIFNLVKSALNGKADDLL
                     NLQKRLNQTKKDIDYILSPIVNRPSLLDRLNKNGKTTDLNKLANLMNQGSDLLDSIPD
                     IPTPKPEKTLTLGKGNGLLSGLLNADGNVSLPKAGETIKEHWLPISVIVGAMGVLMIW
                     LSRRNKLKNKA"
     gene            complement(3015..4373)
                     /gene="gtfB"
                     /locus_tag="ACGEXS_001415"
     CDS             complement(3015..4373)
                     /gene="gtfB"
                     /locus_tag="ACGEXS_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031647 - regulation of protein stability
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HFF8012429.1"
                     /translation="MIKLFDYFNDHSRKLYESFKASKLEEDLTIVLNDNGFLPDDVIS
                     PYQFFADNHNTENMKPRFFNQVTVPAFWEIKGNNNSATINDMGRLRGKIFYQSGERPR
                     IVSRVEWFDDQQRVRFVDYYSKNGIKFAQTVYDLNRKAILKKYMTAEGKEVIYENFVT
                     SDVILDWQGKSYFFPSKLAFALFFIKQLEITEHHFVINSLALPFSVLYNLPSKGSDVL
                     VWQEHCNGNVPGNMQLMCKGDMKRHCNIIIPDKNEYETMLNIADAKVQSRILQGGYLY
                     NYRSRNRYTKEIVILTNSDQLRNIKVLVETLPDFKFHIAAITEMSDKLMQLDQYANVH
                     LYPSINIDRVNELYQLCDIYLDINEGNEILNAVEQAFDYELLILGYRQTAHHAKVTLS
                     EHLFEHNDEITMESKDQLIQMLESLKDQQQFRDALLAQKAHAHEISREQFEQVFKQAL
                     ES"
     gene            complement(4366..5874)
                     /gene="gtfA"
                     /locus_tag="ACGEXS_001416"
     CDS             complement(4366..5874)
                     /gene="gtfA"
                     /locus_tag="ACGEXS_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HFF8012430.1"
                     /translation="MTIYNINFGIGWASSGVEYAQAYRAKLLRRTNQDTKFVFLDFIQ
                     SENIQTLTQNIGFKDQEVIWLYQYFSDISLAPTTYTLDDIERELGHDISFRERNGKIV
                     RLYFNGKSSFVTCYLQNEQKDIVDRAEFVINSMLVRKDFYSYTRIFSEYYAPADNKAK
                     LYMRQFYNQDGSIAYNEYIDGDSSIYVFEDAVLYNKTEFIAYFLQRLNLTRDDIVILD
                     RASDIGQAVLQHKGDSKVGVVIHADHYSNNMMSEQHILWNNYYEYQFSKAKYIDFFIT
                     ATDIQNHMVCRQFEQYQGYRPRVYTIPVGSIDALSYPTLSRKPYAMISASRLANEKHI
                     DWLVKAVIVAKRQVPELTFDIYGEGSEKTRLRKIIDTHRAQDYIRLLGHVKLDEIYND
                     YELFLSASTSEGFGLTLMEAVGSGLGMIGLNVNYGNPTFIRDGENGYLVPFDTDEDSV
                     DDVIAKLAHAIVMYFNNGPQTPHDISYEVAQQFMTQDIILKWETLVQEVLHD"
     gene            complement(5892..8282)
                     /gene="secA2"
                     /locus_tag="ACGEXS_001417"
     CDS             complement(5892..8282)
                     /gene="secA2"
                     /locus_tag="ACGEXS_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HFF8012431.1"
                     /translation="MKHKLDVTINELRLKSIRKIVKRINTWSDEVKSYSDDALKQKTI
                     EFKERLASGVDTLDTLLPEAYAVAREASWRVLGMYPKEVQLIGAIVLHEGNIAEMQTG
                     EGKTLTATMPLYLNALSGKGTYLITTNDYLAKRDFEEMQPLYEWLGLTASLGFVDIVD
                     YEYQKGEKRNIYEHDIIYTTNGRLGFDYLIDNLADSAEGKFLPQLNYGIIDEVDSIIL
                     DAAQTPLVISGAPRLQSNLFHIVKEFVDTLIEDVHFKMKKTKKEIWLLNQGIEAAQSY
                     FNVEDLYSEQAMVLVRNINLALRAQYLFESNVDYFVYNGDIVLIDRITGRMLPGTKLQ
                     AGLHQAIEAKEGMEVSTDKSVMATITFQNLFKLFESFSGMTATGKLGESEFFDLYSKI
                     VVQVPTDKAIQRIDEPDKVFRSVDEKNIAMIHDIVELHETGRPVLLITRTAEAAEYFS
                     KVLFQMDIPNNLLIAQNVAKEAQMIAEAGQIGSMTVATSMAGRGTDIKLGEGVEALGG
                     LAVIIHEHMENSRVDRQLRGRSGRQGDPGSSCIYISLDDYLVKRWSDSNLAENNQLYS
                     LDVQRLSQSNLFNRKVKQIVVKAQRISEEQGVKAREMANEFEKSISIQRDLVYEERNR
                     VLEIDDAENQDFKALAKDVFEMFVNEEKVLTKSRVVEYIYQNLSFQFNKDVACVNFKD
                     KQAVVTFLLEQFEKQLALNRKNMQSAYYYNIFVQKVFLKAIDSCWLEQVDYLQQLKAS
                     VNQRQNGQRNAIFEYHRVALDSFEVMTRNIKKRMVKNICQSMITFDKEGMPVIHFP"
     gene            complement(8272..9231)
                     /gene="asp3"
                     /locus_tag="ACGEXS_001418"
     CDS             complement(8272..9231)
                     /gene="asp3"
                     /locus_tag="ACGEXS_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HFF8012432.1"
                     /translation="MLKNKTFKVTWNYISQTTFMYGSKVSFSNGEVTFINPLMPSGLP
                     IHEWLMLKQFSKYKSAPSLPILRRGQHYKLHFDFDATPAGSVYFIIIFYNKNGTKLST
                     EIVKSNSITIQYPDEAYAYKIKMMNAASTSLIFRCLTITEMTHQYDLEYKSMRVTKIG
                     DDQYGNDMINVIIAEPSDTYPTISNDFLKLFGHVWLVERWMDDNIEGNIKQLKNDLQS
                     QDTLKAINLISYGSKSNVFATYVAQHLGCKVYRTSHEDDDLKGWLTEHVPGNHELKDT
                     NVEVYFKEEQDKHLNYMSRLMNPVRFLDYLDVSKLNGGEVNET"
     gene            complement(9209..10777)
                     /gene="asp2"
                     /locus_tag="ACGEXS_001419"
     CDS             complement(9209..10777)
                     /gene="asp2"
                     /locus_tag="ACGEXS_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HFF8012433.1"
                     /translation="MPRKFRVLQIGGDDLEPIFQHKKGVSWDYFDIGLFEFDSGYVEA
                     IEAIVEAEGRFDFIYIQAPYSETLTNLLQMISEPYNTYVDESFWSVEYEQDENVQKYV
                     VQPLHYRNIEERNNKLEAVSFSGQYGDKVSPKLALVHPNFKGDVVYQGNSELTLSGEF
                     GKEFKPIASWQNNLVYDKDKVIQIWPEFDIDGAVELQYTFRLIQTGADGALIEQIVLT
                     DDMLDSPLEIPAKPFDAYISVTVKARGNGTVHLGPIHKRWSRLDMGQFLLGGSRFVDS
                     QRQEFIYYFHPGDMKPPLNVYFSGYRTAEGFEGYYMMKRMNAPFLLIGDPRVEGGSFY
                     IGSSEYEQGIINVIDETLEKLNFKSHELILSGLSMGSFGALYYGAQLNPQAIIVGKPL
                     VNIGTIAEHMRLLRPEEFGTALDVLVSNEGDTSQASIQALNQKFWQTFQKKSLSQTVF
                     AIAYMQHDDYDPHAFQELLPVLTAHQARVMNRSIPGRHNDDSPTIASWFVNFYNIILE
                     DKFGRVQHAEKQNI"
     gene            complement(10767..12320)
                     /gene="asp1"
                     /locus_tag="ACGEXS_001420"
     CDS             complement(10767..12320)
                     /gene="asp1"
                     /locus_tag="ACGEXS_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HFF8012434.1"
                     /translation="MKYFIPAWYDDQRWWQDTTVPYYQLQNKTEFDDMISLMGMHLEN
                     DLDYQLIVLNHAPNLRTFLHRYDLYETKYSSVFDEIQGFSHHAPQAINYHHLKWPDDV
                     EFVYTPYLLKCVTSEQTYTNIYFSQEGYSIWFEEFERDQLQRRYIFDDRGYLSAIRYF
                     DDQGEASYQEYLTINGDCVLHEDLKNGRVTVSKRYQHHYQQTEYNNMAQLIEEKFQAM
                     IAQQIHEDDHVIVASDARHNRQIANHIPAKSLSYSFFKNRNETVSDEEYQSIVKNAHL
                     IVDSVQLERDLISHQEKYQRENTMIRITPFETRQSPNISSQLMETFIGVWIDGMSDAD
                     LQQMMQRLVDYIAQEDYYRLILLSRHQNDIPMWLRECITSVNEEYQAKQNADVNVSAL
                     MTPEDQDDIIAVKTIHAEHDVVEALRTLRLVIDMSKEPDLYLQISAISAGIPQINGQQ
                     TDYVSDYDNGRIINTVDELDDALNYYLFYLKNWNYAYAYSLKLIDAYASKNIINQLDE
                     LIEGENDAT"
     gene            complement(12331..>13432)
                     /gene="secY2"
                     /locus_tag="ACGEXS_001421"
     CDS             complement(12331..>13432)
                     /gene="secY2"
                     /locus_tag="ACGEXS_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HFF8012435.1"
                     /translation="YNDMQVKHESFFKIAISNMGGDVNTLNIFTLGLGPWLTSMIILM
                     LISYRNMDKYMKQTSLEKHYKERILTLILSVIQSYFVIHEYVSKERVHQDNIYLTILI
                     LVTGTMLLVWLADKNSRYGIAGPMPIVMVSIIKSMMHQKMEYIDASHIVIALLIILVI
                     ITLFILLFIELVEVRIPYIDLMNVSATNMKSYLSWKVNPAGSITLMMSISAFVFLKSG
                     IHFILSMFNKSISDDMPMLTFDSPVGISVYLVIQMLLGYFLSRFLINTKQKSKDFLKS
                     GNYFSGVKPGKDTERYLNYQARRVCWFGLALVTVIIGIPLYFTLFVPHLSTEIYFSVQ
                     LIVLVYISINIAETIRTYLYFDKYKPFLNQYW"
BASE COUNT         4176 a         2610 c         1869 g         4777 t
ORIGIN      
        1 ttatataaat atattatttt atgttcaaat gttcctcatc atatccgttt caattgccat
       61 ctcacacatt ttataaatat gagcaaatgt acttattctc aaacattact gccgagcttt
      121 aattgacgtt atattaacta taaactactt ttccatgact ctacggattc aatgtcacat
      181 gagcgtgata aaatttgttc aataataaag tcatgtttat catctgatct atcaccaaca
      241 gcatcttcta aaacagtaat ataatagtct ttatctacac tttctaatgc cgtgctcaat
      301 acagctccac ttgtagagac acccgttaat actaaatgat taatatcatt tgcacgtaaa
      361 taaacttcca agtaactacc tgtaaatgcg ctaaagcgtc gcttagaaat aatcggctca
      421 tcttctagtg gtgctaaatc ttcaagtatt cgtgtagatg catctgcttc agtaatcgca
      481 tatccttgag ctttaattgt tgaaaacact ttattactcg aggagacatc attaaaatgc
      541 ttatctaaca ctaaacgtat gaaaatgact ggtattcgat gttgtcttgc tgcttcaatt
      601 gctctctgat tcgctttaat aatatttttt attctaggta cactactcgc tataccttct
      661 tgcatatcca aaactaatag cgccgttttt cgagacatct tcattctcct ttacttgtgt
      721 agttctaagt cgttaaattc attataacgt taaaatgatg gacaatctat tcattgcatt
      781 ttgcatatac ttcacaataa attaaaggga aataagacgt cttatatact taaaaaaata
      841 tatagatgct cttcccccaa tataattatg ctttattttt caacttattg cgtcgtgata
      901 accaaatcat tagtacaccc attgcaccaa caattacaga tatcggcaac caatgttctt
      961 ttatcgtttc ccccgcttta ggcaaagata cattaccatc agcatttaat aatccactta
     1021 acaatccatt acctttacca agtgttaacg tcttttctgg ctttggtgtg ggtatatctg
     1081 gaatactgtc taataaatct gatccttgat tcattaaatt tgctaactta tttaaatctg
     1141 tcgttttccc atttttattc aatcgatcta gtaaacttgg acgatttact attggtgata
     1201 aaatataatc tatatctttt ttcgtttgat tgagtctctt ttgtaaattc aataaatcat
     1261 ccgctttacc attcaatgcc gatttaacta aattaaaaat tttattttga tctgtttcta
     1321 ttttagtaat taaatctgcc agtaattttg ccttttgtct ttctatacgt gttgctaaaa
     1381 tcgtttcaat tgcttgtttt ttatctttgg cattattcaa aattgctttt aatatatcat
     1441 ctgaagacgt gtcgccagtt gatgcaaaat gtttcttcaa ttggtcaacg atttggcgat
     1501 ttgataatcc tttattcgtc caatctttag ccaatttatc tgcttcagct tttcctaatt
     1561 tcgtttgtaa aatttgagaa atcaatagcg acttatcttg tgattgatca atcaatgacg
     1621 ttaataaatc atcactcgtt gtcagagata attgatcaat atgacgagta atttgatctg
     1681 caatttgttg atctgtttta ccatcaacac gtatatcttt tagaatttta actgcctcgt
     1741 ctttattaaa tatactttct aaaatgcttt gtgtagcata ctttttatca tcagtacgtg
     1801 caagttcttc caaaataata tttcgttgac tttttatacg ctctttcgtc ttatttactt
     1861 cgctcattaa gtctgatttt tgatttttag gaagttgcgt atttgcaata cgttgatcta
     1921 aagattgtaa cgtattcagt ttatgatatg tgtaatgttg cgttgaggca ttacttttag
     1981 ctaatttttc aatcatagca tgattaattt tatcgcttcc ttgtaattta tcagtgagtc
     2041 gattactatg gctttgattc tcttcatttg aaaggaattt atttaacaca acatgtccag
     2101 aaccatcatt atttggcgtt ttagctactt catgattatt atctgttgta gacactgctg
     2161 gatcttccga tgtatctttc gatgcatctt tcgatttgtg tatttgctga ttcaaatggt
     2221 ctaggtcttc taacgcctta tttaccattg cttcatcatt tttatcatct ttttctccat
     2281 gttttgttgt agccgtttgt gacatatcat ttttcattgc attaagatcg tcctcgccac
     2341 tttgttgacc cctatcaaca tttgaagaaa cctcatttaa atctttaagc aattgatcta
     2401 atttactgtc tatatcactt tgaccgttca tttcagtgtg agaactttta ttgtctttgc
     2461 tatccaactc attagctcgt tttatgattt catctatttg cgatgctgtt ttcgcttcat
     2521 ttagttgtgc tttataatgt tctttagatg aagccgataa ctgttttaat tgctcaattt
     2581 gacgaattgc cttgtcaact ttgtctaata aatcttgctt agataatatc tcttttgtaa
     2641 tttcagtatc cttttcagat gcagcttggg catcgtacgg caagatattc gttaaaatga
     2701 tacttgtcgc catcattgtc gaacacgata actttacata taattgaaac ggtttccctc
     2761 gatatttagc catcaacata ctccttcctc acttacttcc ttcaaagaat tacatactat
     2821 tatatacctg tttacaagaa atttacactt atctatctag ttattgttgt tagtaattat
     2881 ctacttatta cttagcttat atttaagtaa acaaaacaag catgacgtaa tatcatattg
     2941 tccatgtcgc taacatcata ttacgtcaaa tcttttatat taaatgatgt tttattttag
     3001 actgcttttt ccttttagct ttcgagcgcc tgtttaaaaa cttgctcgaa ttgttcacgc
     3061 gagatttcgt gtgcatgtgc tttttgtgct aataaagcat ctcgaaactg ttgttgatct
     3121 ttcaaacttt ctaacatttg tattaattgg tctttacttt ccattgttat ctcatcatta
     3181 tgctcaaata agtgctctga taatgttact ttagcatggt gtgcggtttg acgataacct
     3241 aaaatcaaca actcatagtc aaacgcttgt tccaccgcat ttaaaatttc attaccctca
     3301 ttgatatcaa gataaatatc acataactgg tatagttcat ttaccctgtc aatattaata
     3361 gatgggtata aatgcacatt agcatattga tcaagttgca ttagcttatc agacatctct
     3421 gtaatagcag cgatgtgaaa cttaaaatct ggtaaagttt caaccaatac cttgatgtta
     3481 cgaagttgat ccgagttagt taatattaca atttctttag tatatctatt acgactacga
     3541 tagttatata gatatccgcc ttgtaaaata cgagattgaa cctttgcgtc tgctatattg
     3601 agcatcgttt catattcgtt tttatctgga ataataatat tacaatgtcg tttcatatca
     3661 cctttacaca tcaattgcat atttcccggg acattaccat tacagtgttc ttgccatacc
     3721 aaaacatcac taccttttga tggcaaatta tataacactg aaaatggtag ggctagtgag
     3781 ttaataacga aatgatgttc cgtaatttca agttgcttga taaaaaataa tgcgaatgcg
     3841 agctttgaag ggaaaaagta agacttccct tgccaatcca atatgacatc agatgttaca
     3901 aaattttcat aaatcacttc tttaccttct gctgtcatat atttcttcaa gatcgcttta
     3961 cgatttaaat cgtaaacagt ttgtgcaaat ttaataccat tcttagaata ataatcgaca
     4021 aatcggacac gttgttggtc atcaaaccat tcgacacgac taacaattct agggcgctct
     4081 ccactttgat aaaatatttt acctcgtaga cgtcccatat cattaattgt agccgaattg
     4141 ttgttacctt taatttccca aaaagctggt acagtaacct gattaaaaaa tcgtggtttc
     4201 atattttctg tattatgatt atctgcaaaa aattgatacg gtgatataac atcgtccggt
     4261 aaaaagccat tgtcattgag tacaattgtt aaatcttctt ccaacttact ggctttaaaa
     4321 gactcatata actttcgtga atgatcgtta aagtaatcaa ataatttaat catgtagcac
     4381 ctcttgaact aatgtttccc attttaaaat aatatcttga gtcataaatt gctgtgccac
     4441 ttcataagag atgtcatgtg gtgtctgggg accattgtta aaatacatta caatggcatg
     4501 agctagtttt gcgataacat catccacact atcttcgtcg gtatcaaaag gtaccaagta
     4561 gccattttcc ccatctcgaa taaaggttgg gttaccataa ttcacattta atccaatcat
     4621 acctagtcct gagcctaccg cttccattag tgttaaccca aaaccttcgc tagttgatgc
     4681 agaaagaaat aactcataat cattataaat ttcatcaagt ttaacatgcc ctagtaaacg
     4741 aatataatct tgtgcgcggt gtgtatcaat aattttacgc agtcgcgtct tctcgctacc
     4801 ttctccataa atatcaaatg ttaattctgg cacttgtcgt ttagccacga taaccgcttt
     4861 gacaagccaa tcaatatgtt tctcattcgc taaacgagat gcactaatca tcgcatatgg
     4921 ctttcttgat aatgttggat atgataatgc atcaatgctt cccacaggaa tagtataaac
     4981 acgtgggcga taaccttgat attgctcaaa ttgtcgacaa accatatgat tttgaatatc
     5041 tgttgctgta ataaagaaat caatgtattt agcttttgaa aattgatatt cataataatt
     5101 gttccatagt atatgctgct cactcatcat attattacta taatgatcag catgaatcac
     5161 aacaccaact ttactatcac ctttatgctg caaaacagcc tgaccaatat cagaagcgcg
     5221 gtctaatatg acaatatcgt ctcgggttaa attcaatcgt tgtaaaaagt atgcaataaa
     5281 ttccgttttg ttatacaaca ccgcatcttc aaacacatat atagagctgt ctccatcaat
     5341 atattcgtta taagcgatgg aaccatcttg attataaaat tgtcgcatat ataatttcgc
     5401 tttattatca gctggtgcat aatactcaga aaatatgcgc gtataactat aaaaatcttt
     5461 acgtactaac atactattaa ttacaaattc tgcacgatcc acaatatctt tttgttcatt
     5521 ttgcagataa catgttacaa atgatgattt cccattaaaa tataggcgga ctatcttacc
     5581 atttctttct ctaaaactaa tgtcatgacc aagctcacgt tcaatgtcat ctaacgtgta
     5641 cgttgttggt gctaaagaaa tatcactaaa atactgatac aaccaaataa cttcttgatc
     5701 tttaaaccca atgttttgcg ttaatgtctg tatgttctct gactgtataa aatctaaaaa
     5761 cacaaattta gtgtcttgat ttgtacgtct caataattta gcacggtaag cttgtgcata
     5821 ttcaacaccg ctactcgccc agcctatacc aaagtttata ttatatattg tcatgcgcta
     5881 ccccttttca tttatggaaa atgtataact ggcataccct ctttatcaaa tgtaatcatg
     5941 ctttgacaaa tatttttcac cattcttttt ttgatatttc gtgtcataac ttcaaatgaa
     6001 tctaaggcaa ctctatggta ttcaaaaata gcgttacgtt gcccattttg tcgttgatta
     6061 acgcttgctt ttaattgttg taaatagtcg acttgttcta accaacatga atcaattgct
     6121 ttcaaaaaga ctttttgaac gaaaatatta taataatatg cactttgcat gtttttacga
     6181 ttcaaagcta attgcttttc aaattgctct aataaaaatg tcactactgc ttgcttatct
     6241 ttaaaattaa cacaagccac atctttatta aattggaaac ttaaattttg ataaatatac
     6301 tcgacaacac gcgattttgt tagcaccttt tcctcattta caaacatttc aaatacatct
     6361 ttagctaacg ctttaaaatc ttgattctca gcatcatcta tttctaaaac tcgattgcgt
     6421 tcctcgtata caagatctcg ctgtatacta atgctttttt caaattcatt agccatttca
     6481 cgagctttaa ccccttgttc ttccgagata cgctgcgctt taactacaat ttgcttaact
     6541 ttgcgattaa acaaattact ttgcgataat cgttgtacat ctaatgaata taattgatta
     6601 ttttccgcta aattactatc gctccatcgc ttaactaaat aatcatctag tgaaatatat
     6661 atacaagatg atcccggatc cccttgtcta ccagaacgac cacgtaattg cctgtctaca
     6721 cggctatttt ccatatgttc atgaataata acagctaatc cacctaatgc ttcgacacct
     6781 tcaccaagtt taatatctgt gcctcgacct gccatactag tcgcaacagt catggaacca
     6841 atttgccctg cttcagctat catctgcgct tcttttgcaa cattttgcgc aatgagtaaa
     6901 ttattaggaa tatccatttg gaataatact ttcgaaaagt attcagccgc ttcagcagtt
     6961 ctcgttatga gtaaaaccgg tcgccccgtt tcatgaagtt caactatatc atgaatcatc
     7021 gcgatgtttt tctcatcaac tgaacgaaac actttatctg gttcatcgat acgttgaatc
     7081 gctttatcag ttggtacttg tacgactatt tttgaataca aatcaaagaa ctctgattcg
     7141 cctaattttc ctgtagctgt catacctgaa aatgattcaa aaagtttaaa taaattctgg
     7201 aaggtaattg ttgccataac acttttatct gttgaaacct ccataccttc tttcgcttca
     7261 atagcttggt gaagtccagc ttgcaactta gttcccggta acatacgacc tgtaatacgg
     7321 tcaattaaaa caatatcacc attatataca aagtaatcga cattagattc aaacaaatat
     7381 tgtgcgcgca gtgctaaatt aatattacgc actaggacca tcgcttgttc gctatataaa
     7441 tcttcaacat taaagtatga ttgtgccgct tcaatacctt gatttaacag ccatatttct
     7501 tttttggtct tcttcatttt aaaatgcacg tcttcaatca atgtatctac aaactctttc
     7561 acaatatgaa atagatttga ttgtaatctt ggtgcacccg aaataactaa tggtgtttga
     7621 gcagcatcta aaatgattga atccacttca tcaataatac cgtaatttaa ttgtggtaaa
     7681 aatttccctt ccgcactatc agccaaatta tcaattaaat aatcaaaacc gagacgtcca
     7741 ttagttgtat atataatatc atgttcatat atattacgtt tttccccttt ttgatactca
     7801 taatccacaa tatcaacaaa acctaatgaa gcagttaaac ctaaccattc atataatggt
     7861 tgcatctctt caaaatctcg cttcgctaaa taatcattcg ttgtaattaa atatgttcct
     7921 tttcccgaaa gagcatttaa atataaaggc atcgttgccg ttaatgtttt accttcgcct
     7981 gtttgcatct ccgcaatgtt accttcatgc aatacaatcg ctccgattaa ctgaacttct
     8041 ttaggataca tacctaatac tctccagctc gcttcacgtg ccactgcata agcttcaggt
     8101 aacaatgtat ctagtgtatc aactcctgat gctaaacgtt ctttaaattc tattgtcttt
     8161 tgttttaacg catcatcaga atatgattta acttcatcgc tccatgtatt gatgcgtttc
     8221 actatttttc taatcgactt tagtcttaat tcgtttatcg taacatctag tttatgtttc
     8281 atttacttcc ccaccattca gtttcgatac atctaagtaa tctaaaaatc gtactggatt
     8341 cattaaacgt gacatataat ttagatgttt gtcttgctct tctttaaaat aaacctcgac
     8401 atttgtatct tttagttcat gatttcctgg gacatgttct gtaagccatc cttttaaatc
     8461 atcatcttca tggcttgtac gatacacttt gcaacccaaa tgctgagcga cataagttgc
     8521 aaaaacattt gactttgacc cataactaat caaattaata gcctttaggg tatcttgact
     8581 ttgcaaatca ttctttagtt gcttaatatt tccctcgata ttgtcgtcca tccaacgttc
     8641 aacgagccaa acatgaccaa acagtttcaa aaaatcattc gaaatagttg gataggtgtc
     8701 agatggttct gcaataatga cattgatcat atcatttcca tattggtcat cacctatctt
     8761 cgtcacccgc atgcttttat actctaaatc atattgatgc gtcatctctg tgattgttaa
     8821 acatctaaat ataagactcg tcgatgctgc attcatcatt tttattttat aagcataggc
     8881 ttcatcagga tattgaatcg taatactatt tgactttaca atctcagtac ttagttttgt
     8941 gccattttta ttataaaaaa tgatgataaa atacactgaa ccagcaggcg ttgcatcaaa
     9001 atcaaaatgc aatttataat gctgtcctct acgcaaaatt ggtaaacttg gcgcactttt
     9061 atattttgaa aattgcttta acatcaacca ctcatgaatc ggtaatccag agggcatcaa
     9121 aggatttata aaagtcactt caccatttga aaatgatact ttagagccat acataaatgt
     9181 agtttgtgaa atataattcc aagtaacttt aaatgttttg tttttcagca tgttgaactc
     9241 tcccaaactt gtcttccaaa ataatgttgt aaaaattaac aaaccaactt gcaatggtag
     9301 gtgaatcatc attatgtcgc ccaggaatac tgcgattcat cactcttgct tggtgtgctg
     9361 tcaatacagg taatagctct tgaaatgcat gtggatcata atcatcatgt tgcatatatg
     9421 ctatggcaaa aacagtttgt gacaatgatt tcttttgaaa tgtttgccaa aatttttgat
     9481 ttaatgcctg tatcgacgct tgagatgtat caccttcatt agacaccagg acgtctaatg
     9541 ctgtaccgaa ctcttctggt ctaagtaatc gcatatgttc agcaatcgtt ccaatattaa
     9601 caagtggttt accaacaata attgcctgag gatttaactg agcaccataa tacaaggcac
     9661 caaatgaacc catagataaa ccagataata ttaattcatg tgatttaaaa ttcagctttt
     9721 ctaatgtctc gtcaataaca ttgataatac cttgttcata ttcagatgaa ccgatataaa
     9781 aactaccacc ttcaacacga ggatcgccga taagtaaaaa cggtgcattc atacgtttca
     9841 tcatataata tccttcgaaa ccttccgctg ttcgataacc actaaaatat acgtttagtg
     9901 gcggtttcat atcaccaggg tggaaataat aaataaattc ctgtcgttga ctatctacga
     9961 aacgactacc accaagtaaa aattgaccca tgtctaatct agaccatcgt ttgtgtatag
    10021 gtcctaaatg taccgtcccg ttcccacgcg ccttaacagt tacacttata taagcatcaa
    10081 atggtttcgc aggtatctct aaaggactgt ctaacatatc atcagtcaat acgatttgtt
    10141 caattaatgc accatcagcg ccagtctgaa tcaatctaaa tgtatattgc aactcgaccg
    10201 caccatcaat atcaaattct ggccatattt gaatgacttt atctttatcg taaacgagat
    10261 tattttgcca agatgcgata ggtttaaatt ctttcccaaa ttctccactc aatgtgagct
    10321 ctgaattacc ttggtaaacg acatctcctt taaaattcgg atgcacaagt gctaacttag
    10381 gagaaacctt atctccatac tgtcctgaga agctaactgc ctctaattta ttattacgtt
    10441 cttcaatatt ccggtaatgt aatggttgaa caacgtattt ttggacattt tcgtcttgtt
    10501 catattcaac tgaccaaaat gattcatcaa catacgtatt gtatggttcg cttatcattt
    10561 gtaataaatt cgttaatgtc tccgagtatg gtgcttgaat atagataaaa tcaaagcgcc
    10621 cttctgcttc aacaatcgct tcaatagcct ctacataacc actatcaaat tcaaacaatc
    10681 caatatcgaa gtaatcccaa ctcacacctt ttttgtgttg aaaaataggt tctaaatcgt
    10741 ctcctccaat ttgcaaaact ctaaatttac gtggcatcat tttcaccttc tattaactca
    10801 tcgagctgat taataatatt cttagaagca tatgcatcta ttaattttaa agaataggcg
    10861 tacgcataat tccaattttt caaataaaat aaataataat ttaacgcatc atctaattca
    10921 tcaactgtat ttataatacg gccattgtca taatcagaga cgtaatctgt ttgttgacca
    10981 ttaatttgtg gaatcccagc gctaattgca ctaatttgta aatacaagtc aggttctttt
    11041 gacatatcta tcacaagtcg caacgtccgc aatgcttcta caacatcatg ttcagcatgt
    11101 atcgtcttaa cagcaatgat gtcatcttga tcttcaggtg tcattaatgc tgaaacatta
    11161 acatccgcat tctgtttagc ttggtattcc tcatttaccg acgtaataca ttcacgaagc
    11221 cacatcggta tgtcattttg atggcgcgat aataaaatta aacggtaata atcttcctgt
    11281 gcgatataat ccacaagtcg ttgcatcatt tgttgcaaat cagcgtcact cataccatct
    11341 atccatacac ctataaatgt ttccatcaat tgactactta tattaggtga ttgtctcgtt
    11401 tcaaatggtg tgattcgaat cattgtattc tcccgctgat acttctcttg atgactaatt
    11461 aaatcacgtt ctagttgcac actatcaaca attaaatggg cattctttac gatagattga
    11521 tattcctcat ctgacacagt ttcatttcta tttttaaaaa atgaataact taatgatttc
    11581 gctggaatat gattggctat ttgtcgattg tgcctagcat ctgaagccac aatcacatga
    11641 tcatcttcat gtatttgttg tgcaatcatt gcttgaaatt tttcttcaat tagttgagcc
    11701 atattgttat attctgtttg ttgatagtga tgttgatatc tttttgaaac agtgactctg
    11761 ccatttttca aatcttcatg aagtacacaa tctccattaa tcgttaaata ttcttggtaa
    11821 gaagcctctc cctgatcatc aaaataacgt atcgctgata aataacctct gtcatcaaaa
    11881 atataacgcc gttgtaactg atctctttca aattcttcaa accaaattga atacccttct
    11941 tgactaaaat aaatatttgt ataggtctgt tcactcgtca cacattttaa taaatacggt
    12001 gtgtacacaa actcaacatc atccggccat tttaagtgat gataattaat cgcttgtggc
    12061 gcatggtgac tgaatccttg aatttcatca aacacagacg aatactttgt ctcatataag
    12121 tcatatcgat gtaaaaatgt tcttaaattt ggtgcatgat tgagaacaat cagttgataa
    12181 tctaagtcat tttcaaggtg cattcccatt aaactaatca tatcgtcaaa ttccgtctta
    12241 ttttgtagtt gataatacgg cacagtcgtg tcttgccacc atcgttggtc atcgtaccaa
    12301 gctggaataa agtatttcat aattacctcc ttaccaatac tggtttaaaa atggcttata
    12361 tttatcaaaa tataaatatg tacgaattgt ttctgcaata ttaatactga tgtaaactaa
    12421 tacaatcagt tgtactgaga aataaatttc agtagataaa tgcggtacaa acaatgtgaa
    12481 ataaagcggt ataccaataa tgactgtaac taatgccaat ccaaaccaac atacgcgtcg
    12541 tgcttgataa tttaaataac gttctgtatc cttaccaggt ttaactcctg aaaaataatt
    12601 gccactcttt aagaaatctt tggatttttg tttagtattg attaaaaatc tcgataaaaa
    12661 ataacccaat aacatttgaa tcactaaata tactgaaata cctactggac tatcaaatgt
    12721 cagcattggc atgtcatctg atatgctttt attaaacata gataaaataa aatgaatgcc
    12781 actttttaag aaaacaaaag ctgaaatact catcattaaa gtaatactgc ctgcagggtt
    12841 aactttccaa gataaataag atttcatatt tgttgcggaa acgttcatta aatcgatata
    12901 tggtattctc acttctacta attcaataaa taataagata aacaatgtga ttatcacaag
    12961 gatgattaac aacgcaatca caatatgact tgcatctata tattccattt tttgatgcat
    13021 cattgattta ataatactaa ccattacaat cggcattggt cctgcgatgc cgtagcgact
    13081 atttttgtca gctaaccaaa ctaataacat cgttccagta accaaaatca atattgttaa
    13141 gtaaatattg tcttgatgaa cacgttcttt cgaaacatat tcatgaatca caaaataact
    13201 ttgaataaca cttaaaatta atgttaagat gcgctcttta taatgcttct caagacttgt
    13261 ttgtttcatg tatttatcca tattccgata agaaatcagc atcaagataa tcattgatgt
    13321 taaccacgga cctaatccta aagtgaaaat gtttaaagta ttaacgtctc cacccatatt
    13381 agaaatagct attttaaaaa atgactcatg ttttacttgc atatcgttat ag
//
LOCUS       DAXWFK010000065        13422 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.065, whole genome shotgun sequence.
ACCESSION   DAXWFK010000065 DAXWFK010000000
VERSION     DAXWFK010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13422
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.065"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            67..576
                     /locus_tag="ACGEXS_001422"
     CDS             67..576
                     /locus_tag="ACGEXS_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcG family protein"
                     /protein_id="HFF8012436.1"
                     /translation="MILGLALIPSKSFQEAVDSYRKRYDKQYSRIKPHVTIKAPFEIK
                     DGDLDSVIEQVRARINGIPAVEVHATKASSFKPTNNVIYFKVAKTDDLEELFNRFNGE
                     DFYGEAEHVFVPHFTIAQGLSSQEFEDIFGQVALAGVDHKEIIDELTLLRFDDDEDKW
                     KVIETFKLA"
     gene            complement(689..1879)
                     /locus_tag="ACGEXS_001423"
     CDS             complement(689..1879)
                     /locus_tag="ACGEXS_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8012437.1"
                     /translation="MQDSSLNNYANHKNFILMLIILFLMEFARGMYILSYINFLPTVT
                     SIAVAITSLAFSIHFIADASTNFVIGFLLKKFGTKIVLTTGFILAFTSLFLVIWFPAS
                     PFVIIFSAMMLGIAVSPIWVIMLSSVEEDKRGKQMGYVYFSWLLGLLVGMVFMNLLIK
                     VHPTRFAFMMSLVVLIAWILYYFVDVKLTNYNTRPVKAQLRQIVDVTKRHLLLFPGIL
                     LQGAAIAALVPILPTYATKVINVSTIEYTVAIIIGGIGCAVSMLFLSKLIDNRSRNFM
                     YGVILSGFILYMILIFTLSMIVNIHILWIIALAIGLMYGILLPAWNTFMARFIKSDEQ
                     EETWGVFNSIQGFGSMIGPLFGGLITQFTNNLNNTFYFSALIFLVLAVFYGSYFIVNR
                     EKAK"
     gene            complement(1857..3032)
                     /locus_tag="ACGEXS_001424"
     CDS             complement(1857..3032)
                     /locus_tag="ACGEXS_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diglucosyl diacylglycerol synthase"
                     /protein_id="HFF8012438.1"
                     /translation="MVTQNKKILIITGSFGNGHMQVTQSIVNQLNDMNLDHLSVIEHD
                     LFMEAHPILTSICKKWYINSFKYFRNMYKGFYYSRPDKLDKCFYKYYGLNKLINLLIK
                     EKPDLILLTFPTPVMSVLTEQFNINIPVATVMTDYRLHKNWITPYSTRYYVATKETKQ
                     DFIDVGIDPSTVKVTGIPIDNKFETPINQKQWLIDNNLDPDKQTILMSAGAFGVSKGF
                     DTMITDILAKSANAQVVMICGKSKELKRSLTAKFKSNENVLILGYTKHMNEWMASSQL
                     MITKPGGITITEGFARCIPMIFLNPAPGQELENALYFEEKGFGKIADTPEEAIKIVAS
                     LTNGNEQLTNMISTMEQDKIKYATQTICRDLLDLIGHSSQPQEIYGKVPLYARFFVK"
     gene            3465..4946
                     /locus_tag="ACGEXS_001425"
     CDS             3465..4946
                     /locus_tag="ACGEXS_001425"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFF8012439.1"
                     /translation="MDASTLFKKVKVKRVLGSLEQQIDDITTDSRTAREGSIFVASVG
                     YTVDSHKFCQNVADQGCKLVVVNKEQSLPANVTQVVVPDTLRVASILAHTLYDYPSHQ
                     LVTFGVTGTNGKTSIATMIHLIQRKLQKNSAYLGTNGFQINETKTKGANTTPETVSLT
                     KKIKEAVDAGAESMTLEVSSHGLVLGRLRGVEFDVAIFSNLTQDHLDFHGTMEAYGHA
                     KSLLFSQLGEDLSKEKYVVLNNDDSFSEYLRTVTPYEVFSYGIDEEAQFMAKNIQESL
                     QGVSFDFVTPFGTYPVKSPYVGKFNISNIMAAMIAVWSKGTSLETIIKAVENLEPVEG
                     RLEVLDPSLPIDLIIDYAHTADGMNKLIDAVQPFVKQKLIFLVGMAGERDLTKTPEMG
                     RVACRADYVIFTPDNPANDDPKMLTAELAKGATHQNYIEFDDRAEGIKHAIDIAEPGD
                     TVVLASKGREPYQIMPGHIKVPHRDDLIGLEAAYKKFGGGPVD"
     gene            4939..5190
                     /locus_tag="ACGEXS_001426"
     CDS             4939..5190
                     /locus_tag="ACGEXS_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueH family protein"
                     /protein_id="HFF8012440.1"
                     /translation="MINKRFIDEGKTIDVYLFEALNNQIIIAIPDWFWSYQMAMTLDE
                     ETCFEAILMQLFVFKEEEEAESIASQLTDWIETYKKEKD"
     gene            5190..6752
                     /locus_tag="ACGEXS_001427"
     CDS             5190..6752
                     /locus_tag="ACGEXS_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFF8012441.1"
                     /translation="MNLKQEVESRKTFAIISHPDAGKTTLTEKLLYFSGAIREAGTVK
                     GKKTGKFATSDWMKVEQERGISVTSSVMQFDYDDYKINILDTPGHEDFSEDTYRTLMA
                     VDSAVMVIDCAKGIEPQTLKLFKVCKMRGIPIFTFINKLDRVGKEPFELLDEIEETLN
                     IETYPMNWPIGMGQSFFGIIDRKSKTIEPFRDEENILHLNDDFELEEDHAITNDSDFE
                     QAIEELMLVEEAGEAFDNDALLSGDLTPVFFGSALANFGVQNFLNAYVDFAPMPNARQ
                     TKEDVEVSPFDDSFSGFIFKIQANMDPKHRDRIAFMRVVSGAFERGMDVTLQRTNKKQ
                     KITRSTSFMADDKETVNHAVAGDIIGLYDTGNYQIGDTLVGGKQTYSFQDLPQFTPEI
                     FMKVSAKNVMKQKHFHKGIEQLVQEGAIQYYKTLHTNQIILGAVGQLQFEVFEHRMKN
                     EYNVDVVMEPVGRKIARWIENEDQITDKMNTSRSILVKDRYDDLVFLFENEFATRWFE
                     EKFPEIKLYSLL"
     regulatory      6872..6978
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00080"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="yybP-ykoY riboswitch is a manganese riboswitch that
                     directly binds Mn; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="manganese"
                     /db_xref="RFAM:RF00080"
     gene            7054..7857
                     /locus_tag="ACGEXS_001428"
     CDS             7054..7857
                     /locus_tag="ACGEXS_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFF8012442.1"
                     /translation="MLMDPSLILPYLWVLVVLVFLEGLLAADNAIVMAVMVKHLPPEQ
                     RKKALFYGLLGAFVFRFLALFLISIIANFWFIQAAGAVYLIYMSIKNLWQFFKHPEIE
                     SPEAGDDHHYDESGEEIKASNKSFWGTVLKIEFADIAFAIDSMLAALAIAVTLPKVGI
                     HFGGMDLGQFVVMFLGGMIGVILMRYAATWFVELLNKYPGLEGAAFAIVGWVGVKLVV
                     MVLAHPDIAVLPEHFPHGVLWQSIFWTVLIGLVIIGWLGSVVKNKKPHK"
     gene            8076..10400
                     /locus_tag="ACGEXS_001429"
     CDS             8076..10400
                     /locus_tag="ACGEXS_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFF8012443.1"
                     /translation="MEWTLVDIGKKHVIPKSQYRRKRREFFHNEDREENLNQHQDKQN
                     IDNTTSKKADKQIHKDSIDKHERFKNSLSSHLEQRNRDVNENKAEESKSNQDSKSAYN
                     RDHYLTDDVSKKQNSLDSVDQDTEKSKYYEQNSEATLSTKSTDKVESTEMRKLSSDKN
                     KVGHEEQHVLSKPSEHDKETRIDSESSRTDSDSSMQTEKIKKDSSDGNKSSNLKSEVI
                     SDKSNTVPKLSESDDEVNNQKPLTLPEEQKLKRQQSQNEQTKTYTYGDSEQNDKSNHE
                     NDLSHHIPSISDDKDNVMRENHIVDDNPDNDINTPSLSKTDDDRKLDEKIHVEDKHKQ
                     NADSSETVGYQSQSTASHRSTEKRNISINDHDKLNGQKTNTKTSANNNQKKATSKLNK
                     GRATNNNYSDILKKFWMMYWPKLVILMGIIILIVILNAIFNNVNKNDRMNDNNDADAQ
                     KYTTTMKNANNTVKSVVTVENETSKDSSLPKDKASQDEVGSGVVYKKSGDTLYIVTNA
                     HVVGDKENQKITFSNNKSVVGKVLGKDKWSDLAVVKATSSDSSVKEIAIGDSNNLVLG
                     EPILVVGNPLGVDFKGTVTEGIISGLNRNVPIDFDKDNKYDMLMKAFQIDASVNPGNS
                     GGAVVNREGKLIGVVAAKISMPNVENMSFAIPVNEVQKIVKDLETKGKIDYPDVGVKM
                     KNIASLNSFERQAVKLPGKVKNGVVVDQVDNNGLADQSGLKKGDVITELDGKLLEDDL
                     RFRQIIFSHKDDLKSITAKIYRDGKEKEINIKLK"
     gene            10417..11775
                     /locus_tag="ACGEXS_001430"
     CDS             10417..11775
                     /locus_tag="ACGEXS_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFF8012444.1"
                     /translation="MSIFSQFLKRSSPQQGIVLYYIVAIVIAFLLLNLPYVHKPGVEV
                     NPIDTLFVAVSGISVTGLSPISIVDTYSTFGQLIILVILNIGGIGVMAIGTMLWVVLG
                     KHIGIRERQLIMLDNNKNTMSGTVKLIIDIVKSIFVIELVGAMLLAFYFYRDNPDLKY
                     AIMQGVFVSISATTNGGLDITGKSLIPYAHDYFVQAIVIFLIILGSIGFPVLLEVKAY
                     IQNRVTNFRFSLFTKITTSTYLFLFIVGVLAILLFEHNHAFKGLSWHQSLFYSLFQSA
                     TTRSAGLQTIDVTTLSDPTNIIMGILMFIGSSPSSVGGGIRTTTFAILILFLINFSNN
                     ADKTSIKVYNREVHIMDIQRSFAVFTMATILTFLGMLIISATENGKLTFLQVFFEVMS
                     AFGTCGLSLGVTSDISDISKVVLMILMFIGRVGLISFIIMIAGRREPDKFHYPKERIQ
                     IG"
     gene            11914..>13422
                     /locus_tag="ACGEXS_001431"
     CDS             11914..>13422
                     /locus_tag="ACGEXS_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFF8012445.1"
                     /translation="MEKNENINVEILTTSDMHSHFLNGDYGSNIYRAGTYVNQVRAQN
                     HRVILLDSGGSLAGSLAAYYYAIVAPYKRHPMIKLMNRMHYDASGVSPSEFKFGLSFL
                     TRSIALARFPWLSANIEYNVTKEPYFSTPYCIKHFGDLKIAIVGVTADGLMENEYSEM
                     EQDVSIEKTLVASKRWIRYIHEVEEPDFLIVIYHGGLNKISNSTKNKKASSNEAEKLM
                     EELGVIDLMITAHQHQTIVGQDHETYYVQAGQDAKELVHLSINFKKRTTTYDVESIDS
                     KVIDLNEYEEDQELLDLTFYDRKAVAYWSQEIISDKGLMLSVNGLQDLVCQTHPFSQL
                     LHDAIHLAFDNDITCVHVPMNGEKGLSGQIRNEDLYHAYPYPDKPMDMTISGQNIKDI
                     LEYSYSHLDFVNEQLSLTIIDETLCTMWQGFNYEIDMNQEPGQRVMLDQIDLTKSYRV
                     TMTDYCYRNYKNYLKNAIIHESYDETMSTLIAEKLRDPNYRISCSDNFIVKNR"
BASE COUNT         4792 a         1868 c         2491 g         4271 t
ORIGIN      
        1 tttaatgtaa cggttttcaa agaaatttga tataatagca ataggttaaa caaaggagga
       61 attcagatga ttttaggatt agcattaatt ccatcaaagt catttcaaga agcggtggat
      121 tcttaccgta aaagatatga taaacagtat tcacgaatta aaccacatgt gacaattaaa
      181 gcgccatttg aaattaaaga tggtgattta gattctgtca ttgaacaggt tagagctcgt
      241 attaatggta taccagcagt agaagttcat gctacaaaag cttctagctt caaaccaacg
      301 aacaatgtga tttactttaa agttgcgaag acggacgact tagaagaatt gtttaatcgc
      361 tttaatggag aagatttcta tggagaagct gaacatgttt ttgtgccaca ctttacaata
      421 gcacaaggac tatctagcca agaattcgaa gatatttttg gtcaagtagc attagctggg
      481 gtagaccata aagaaattat cgatgaatta actttgttac gttttgacga tgacgaagat
      541 aaatggaaag ttattgaaac gtttaaatta gcttaagtaa cataatagta ttgttaatcg
      601 tagtatgttt gaattaataa gaaaatggtc atttttattg aatgtaataa aaatgaccat
      661 tttctttatt ttaaaatacg ttttaacctt acttagcttt ttctctattt actataaagt
      721 agcttccata aaatacagct aagactaaaa agattaatgc cgagaaataa aatgtattgt
      781 ttaaattgtt ggtaaattgt gtaattaatc cgccaaataa tggccctatc attgagccga
      841 atccttggat actattaaaa acaccccaag tttcttcttg ttcatctgat ttgataaatc
      901 gtgccataaa ggtattccat gctggtaata agatgccata cattagaccg atagctaaag
      961 cgataatcca caagatgtga atattaacaa tcatagatag agtaaaaatt aatatcatgt
     1021 ataaaataaa tccgcttaga ataacaccat acataaagtt tctgctgcgg ttatctatta
     1081 gtttcgataa aaatagcatc gaaactgcac agccgatacc accaataatg attgcaacag
     1141 tatattcaat tgtgcttacg ttaataacct tagtagcata tgttggtaat ataggaacta
     1201 gggcagcaat tgcggcacct tgtaaaagaa taccagggaa caacaataaa tggcgctttg
     1261 tcacatcaac aatttgtctc aattgagctt taactggacg agtattataa tttgttaact
     1321 ttacatcgac aaaataatat aatatccatg caattaaaac gactaaagac atcatgaagg
     1381 caaagcgtgt tgggtgcact ttgataagta gattcataaa aaccatacct accaataggc
     1441 ctaacaacca tgaaaaataa acatagccca tttgtttgcc acgtttatct tcttcaacac
     1501 tggataacat aatgacccaa ataggactaa ctgcaatacc gagcatcata gcactaaata
     1561 tgattacaaa aggtgatgct ggaaaccaaa taactaaaaa taaacttgta aatgctaaaa
     1621 taaatccagt cgttaaaacg atttttgtgc cgaatttttt cagtaaaaat cctataacaa
     1681 agtttgtaga tgcatcagca ataaaatgta ttgaaaatgc tagagacgtt attgctacag
     1741 caatggatgt aactgttggc aagaaattaa tatagcttag gatatacatg cctctcgcaa
     1801 attccattaa aaataagata ataagcatta aaatgaaatt tttatgatta gcgtaattat
     1861 ttaacgaaga atcttgcata taaaggaacc tttccataaa tctcttgtgg ttgtgatgaa
     1921 tgaccgatta aatcaagtaa gtctcgacat attgtctgtg tagcatactt aattttatct
     1981 tgttccattg tactaatcat gttagttaat tgctcattac cgttagttaa acttgctaca
     2041 atttttattg cttcttctgg agtatcagcg attttaccaa aacctttttc ttcaaagtaa
     2101 agggcatttt caagctcttg accaggtgca ggatttagga aaatcattgg aatacaacgg
     2161 gcgaaacctt cagttattgt gataccacca ggtttcgtaa tcataagttg acttgatgcc
     2221 atccattcat tcatgtgttt ggtataacct agaatcaata cattctcgtt agatttaaac
     2281 ttagctgtta aagaacgctt tagctctttg ctcttaccac aaatcataac tacttgtgca
     2341 tttgcacttt tcgctaatat atcagtaatc atcgtgtcaa aacctttaga tacaccaaat
     2401 gcaccagctg acattaaaat agtttgctta tctggatcta agttgttgtc tattaaccac
     2461 tgcttttgat taataggcgt ttcaaatttg ttatcaatag gaatacctgt cactttaact
     2521 gttgaaggat caatacctac gtctatgaag tcttgtttcg tttcttttgt tgccacataa
     2581 tatcttgttg aatacggcgt aatccagttt ttatgtaagc gatagtctgt catcactgta
     2641 gcaactggaa tattaatgtt aaattgctca gttagtaccg acataactgg tgtaggaaac
     2701 gttaataata ttaaatctgg cttttctttt atcaataaat taattaactt attaagtcca
     2761 tagtatttgt aaaaacattt gtctagttta tctgggcggc tgtaataaaa ccctttgtac
     2821 atatttctaa aatatttaaa gctattgata taccattttt tacaaataga agtcaaaatt
     2881 ggatgagctt ccataaataa atcgtgctca atgacgctta aatggtctag attcatatca
     2941 ttaagttgat taacgatact ctgtgtaact tgcatatgac cgttaccgaa tgagccagta
     3001 ataatcaata tctttttatt ttgagtaacc attaatagcc accctccgtt agtttgaaaa
     3061 ttttatttaa gtgtaactta ttttacggca ttataaaaga aataaagacg caaagtcgtt
     3121 acatttatag caattttaat ctatagatga attgatacaa aataaaacgt tattttataa
     3181 agcaatttat tgttctatgt tttatttgta tatttaaaat tatccagtat acaattatag
     3241 catatttttg gaaacaatta tgatattata ccatgttaca agatggtttt aataatttaa
     3301 gatgagccat aattgtaaaa ctaattcata ataccgtatg ttttattttt aatagtagaa
     3361 attagaaaat gctgattagt aggatataac agtgaaatta taaatttatt aacatcaaca
     3421 aaacgtgtat aataaacata ttgtagaaaa aggagcggtt cagtttggat gcaagtacgt
     3481 tgtttaagaa agtaaaagta aagcgtgtat tgggttcttt agaacaacaa atagatgata
     3541 tcactactga ttcacgtaca gcgagagaag gtagcatttt tgtcgcttca gttggatata
     3601 ctgtagacag tcataagttc tgtcaaaatg tagctgatca agggtgtaag ttggtagtgg
     3661 tcaataaaga acaatcatta ccagctaacg taacacaagt ggttgtgccg gacacattaa
     3721 gagtagctag tattctagca cacacattat atgattatcc gagtcatcag ttagtgacat
     3781 ttggtgtaac gggtacaaat ggtaaaactt ctattgcgac gatgattcat ttaattcaaa
     3841 gaaagttaca aaaaaatagt gcatatttag gaactaatgg tttccaaatt aatgaaacaa
     3901 agacaaaagg tgcaaatacg acaccagaaa cagtttcttt aactaagaaa attaaagaag
     3961 cagttgatgc aggcgctgaa tctatgacat tagaagtatc aagccatggc ttagtattag
     4021 gacgactgcg aggcgttgaa tttgacgttg caatattttc aaatttaaca caagaccatt
     4081 tagattttca tggcacaatg gaagcatacg gacacgcgaa gtctttattg tttagtcaat
     4141 taggtgaaga tttgtcgaaa gaaaagtatg tcgtgttaaa caatgacgat tcattttctg
     4201 agtatttaag aacagtgacg ccttatgaag tatttagtta tggaattgat gaggaagccc
     4261 aatttatggc taaaaatatt caagaatctt tacaaggtgt cagctttgat tttgtaacgc
     4321 cttttggaac ttacccagta aaatcgcctt atgttggtaa gtttaatatt tctaatatta
     4381 tggcggcaat gattgcggtg tggagtaaag gtacatcttt agaaacgatt attaaagctg
     4441 ttgaaaattt agaacctgtt gaagggcgat tagaagtttt agatccttcg ttacctattg
     4501 atttaattat cgattatgca catacagctg atggtatgaa caaattaatc gatgcagtac
     4561 agccttttgt aaagcaaaag ttgatatttt tagttggtat ggcaggcgaa cgtgatttaa
     4621 ctaaaacgcc tgaaatgggg cgagttgcct gtcgtgcaga ttatgtcatt ttcacaccgg
     4681 ataatccggc aaatgatgac ccgaaaatgt taacggcaga attagccaaa ggtgcaacac
     4741 atcaaaacta tattgaattt gatgatcgtg cagaagggat aaaacatgca attgacatag
     4801 ctgagcctgg ggatactgtc gttttagcat caaaaggaag agaaccatat caaatcatgc
     4861 cagggcatat taaggtgcca catcgagatg atttaattgg ccttgaagca gcttacaaaa
     4921 agttcggtgg tggccctgtt gattaataaa agatttattg atgaaggtaa aactattgat
     4981 gtttatttat tcgaagcatt aaataaccag ataatcattg ctataccaga ttggttttgg
     5041 tcatatcaga tggcaatgac attagatgaa gaaacttgtt ttgaagcaat actcatgcaa
     5101 ttgtttgttt ttaaagaaga ggaagaggca gaatcgattg catcacaact aacagattgg
     5161 atagaaacat ataaaaagga gaaagactaa tgaacttaaa gcaagaagtt gagtctagaa
     5221 agacttttgc gattatttca catcccgatg cagggaaaac aacgttaact gaaaaactat
     5281 tgtacttcag tggtgctatt cgtgaagcgg gtacagttaa agggaagaag actggtaaat
     5341 ttgcgacaag tgactggatg aaagttgaac aagagcgtgg tatttctgta actagttcag
     5401 taatgcaatt tgattacgat gattataaaa tcaatatctt agatacacca ggacatgaag
     5461 acttttcaga agatacgtat agaacattaa tggcagttga cagtgctgtc atggtcatag
     5521 actgtgcaaa aggtattgaa ccacaaacat tgaagttatt taaagtttgt aaaatgcgtg
     5581 gtattccaat ctttacattc attaataaat tagaccgagt aggtaaagaa ccatttgaat
     5641 tattagatga aatcgaagag acattaaata ttgaaacata ccctatgaat tggccaattg
     5701 gtatgggaca aagtttcttt ggcatcattg atagaaagtc taaaacaatt gaaccattta
     5761 gagatgaaga aaatatatta catttgaatg atgattttga gttggaagaa gatcatgcaa
     5821 ttacaaatga tagtgatttt gaacaagcga ttgaagaatt aatgttggtt gaagaagcgg
     5881 gtgaagcctt tgataatgac gcgctgttga gtggagactt aacacctgta tttttcggtt
     5941 cagctttagc taactttggt gtacaaaatt tcttaaatgc atatgttgat tttgcgccaa
     6001 tgccaaatgc gagacaaaca aaagaagacg ttgaagtaag cccgtttgat gattcatttt
     6061 caggatttat ctttaaaatt caagccaaca tggaccctaa acaccgtgat agaattgcct
     6121 ttatgcgtgt cgttagtggt gcatttgaac gtggtatgga tgttactttg caacgtacta
     6181 ataaaaagca aaagatcaca cgttcaacgt catttatggc agacgataaa gaaactgtga
     6241 atcatgctgt agcaggcgat atcattggac tatatgatac tggtaattat caaattggag
     6301 atactttagt tggtggaaaa caaacctaca gtttccaaga tttaccacaa tttacgccag
     6361 aaatttttat gaaagtttct gctaaaaacg tcatgaaaca gaagcatttc cataaaggta
     6421 ttgaacaatt agtacaagaa ggtgcgattc aatactataa aacattacac acaaaccaaa
     6481 ttattttagg tgctgttggt cagttacaat ttgaagtttt cgaacataga atgaaaaacg
     6541 aatataatgt tgatgttgtt atggagccag taggccgtaa aattgcacgt tggattgaaa
     6601 atgaagacca aattacagat aagatgaaca catcaagatc gattttagtg aaagatagat
     6661 atgacgattt agtattctta tttgaaaatg aatttgcaac aagatggttt gaagagaaat
     6721 tccctgaaat taaattgtat agtttacttt aacagctcaa ttgtataatc gaatttgtta
     6781 cattaaaaat aattgtttcg ttgaagaaaa ataaattgta tattttaaaa gaaaaaggta
     6841 tactatgatg tatcaaatga ataacctatg gcattttgtc agaggggagt aacttaagaa
     6901 tcatgaccgt ataaatgatt cgacacttta tcgtcattac gaagatatct tccggtaaag
     6961 tgggcaattt aaattgctta gtgagacctt tgctatttat ttagcatagg tctttttgtt
     7021 tgtacttaac ttatttattt aaaggagttg tacatgttaa tggatccaag tttgatctta
     7081 ccttatttat gggtacttgt cgttttagta tttttagaag gcttattagc agcagataac
     7141 gcgattgtta tggctgtaat ggttaagcac ttaccacccg aacaacgtaa aaaagctttg
     7201 ttttacggtt tgttaggtgc atttgtattt agatttttag cattattctt aattagtatt
     7261 atcgcgaact tttggtttat tcaagctgca ggagcggttt acttaattta tatgtcaatc
     7321 aaaaatctgt ggcagttctt taaacaccca gaaattgaaa gtcctgaagc tggagatgat
     7381 catcattatg atgaatctgg tgaagagatt aaagcaagta acaaatcatt ctggggaact
     7441 gtgttgaaaa tagaatttgc agatatcgca tttgccattg attctatgct tgctgcttta
     7501 gctattgctg taacacttcc taaagttggt attcactttg gtggtatgga cttaggtcag
     7561 ttcgtagtca tgttcctagg tggaatgatt ggtgttattc taatgcgtta tgcagcaaca
     7621 tggtttgtag agctattaaa caaatatcca ggacttgaag gtgcagcctt cgcgatcgtt
     7681 ggttgggtag gtgttaaatt agttgtcatg gtattagcgc acccagacat cgctgtattg
     7741 cctgagcact tcccacatgg cgtattatgg caatctattt tctggacagt actaattgga
     7801 ttagtaatta tcggttggtt aggttcagtt gttaaaaata aaaaaccgca taaataattg
     7861 atgtgaagcg gacaatctta atttagttta aggttgtcct ttttcattta attgagtgat
     7921 ttatgaaaaa tggattttga agaatgtgaa tcaaaagatg cgatatagta ttaagaaaat
     7981 gtgcctttta tatttagcat tttttcaata gaaattatat agattttaaa gcaaattagg
     8041 tgttaatgtg tcataatgat aagtgatttt attgaatgga gtggacatta gtggatattg
     8101 gtaaaaaaca tgtaattcct aaaagtcagt accgacgtaa gcgtcgtgaa ttcttccaca
     8161 acgaagacag agaagaaaat ttaaatcaac atcaagataa acaaaatata gataatacaa
     8221 catcaaaaaa agcagataag caaatacata aagattcaat tgataagcac gaacgtttta
     8281 aaaatagttt atcatcgcat ttagaacaga gaaaccgtga tgttaatgag aataaagctg
     8341 aagaaagtaa aagtaatcag gatagtaagt cagcatataa cagagatcat tatttaacag
     8401 acgatgtatc taaaaaacaa aattcattag attcagtgga ccaagataca gagaaatcaa
     8461 aatattatga gcaaaattct gaagcgactt tatcaactaa atcaaccgat aaagtagaat
     8521 caactgaaat gagaaagcta agttcagata aaaacaaagt tggtcatgaa gagcaacatg
     8581 tactttctaa accttcagaa catgataaag agactagaat tgattctgag tcttcaagaa
     8641 ctgattcaga cagctcgatg cagacagaga aaataaaaaa agacagttca gatggaaata
     8701 aaagtagtaa tctgaaatct gaagtaatat cagacaaatc aaatacagta ccaaaattgt
     8761 cggaatctga tgatgaagta aataatcaga agccattaac tttaccggaa gaacagaaat
     8821 tgaaaagaca gcaaagtcaa aatgagcaaa caaaaaccta tacatatggt gatagcgaac
     8881 aaaatgacaa gtctaatcat gaaaatgatt taagtcatca tataccatcg ataagtgatg
     8941 ataaagataa cgtcatgaga gaaaatcata ttgttgacga taatcctgat aatgatatca
     9001 atacaccatc attatcaaaa acagatgacg atcgaaaact tgatgaaaaa attcatgttg
     9061 aagataaaca taaacaaaat gcagactcgt ctgaaacggt gggatatcaa agtcagtcaa
     9121 ctgcatctca tcgtagcact gaaaaaagaa atatttctat taatgaccat gataaattaa
     9181 acggtcaaaa aacaaataca aagacatcgg caaataataa tcaaaaaaag gctacatcaa
     9241 aattgaacaa agggcgcgct acgaataata attatagtga cattttgaaa aagttttgga
     9301 tgatgtattg gcctaaatta gttattctaa tgggtattat tattctaatt gttattttga
     9361 atgccatttt taataatgtg aacaaaaatg atcgcatgaa tgataataat gatgcagatg
     9421 ctcaaaaata tacgacaacg atgaaaaatg ccaataacac agttaaatcg gtcgttacag
     9481 ttgaaaatga aacatcaaaa gattcatcat tacctaaaga taaagcatct caagacgaag
     9541 tgggatcagg tgttgtatat aaaaaatctg gagatacgtt atatattgtt acgaatgcac
     9601 acgttgtcgg tgataaagaa aatcaaaaaa taactttctc gaataataaa agtgttgttg
     9661 ggaaagtgct tggtaaagat aaatggtcag atttagctgt tgttaaagca acttcttcag
     9721 acagttcagt gaaagagata gctattggag attcaaataa tttagtgtta ggagagccaa
     9781 tattagtcgt aggtaatcca cttggtgtag actttaaagg cactgtgaca gaaggtatta
     9841 tttcaggtct gaacagaaat gttcctattg atttcgataa agataataaa tatgatatgt
     9901 tgatgaaagc tttccaaatt gatgcatcag taaatccagg taactcgggt ggtgctgtcg
     9961 tcaatagaga aggaaaatta ataggtgtag ttgcagctaa aattagtatg ccaaacgttg
    10021 aaaatatgtc atttgcaata cctgttaatg aagtacaaaa gattgtaaaa gatttagaaa
    10081 caaaaggtaa aattgactat cccgatgtag gtgttaaaat gaagaatatt gccagtctaa
    10141 atagttttga aagacaagca gttaaattgc caggaaaagt taagaacggt gttgttgtag
    10201 atcaagttga caacaatggt ttagcagatc aatctggtct gaaaaaaggt gatgtaatta
    10261 ctgaattaga tggcaaactt ttagaagatg atttacgctt taggcagatt atatttagtc
    10321 ataaagatga cttgaaatca attacagcga agatttatag agatggtaaa gagaaagaaa
    10381 ttaatattaa actaaaataa tgttgaggtg aacaacgtgt caatttttag ccagttttta
    10441 aaaagatcaa gccctcaaca aggtattgta ttgtactata tcgtcgcaat tgtcattgca
    10501 tttttattat taaacttacc gtatgttcat aaaccaggtg tagaagtaaa tccaattgac
    10561 acattatttg ttgccgtatc cggaattagt gttactggat tgtctccgat aagtattgtc
    10621 gatacctatt ctacatttgg acaattaatt atcctcgtga tattaaatat tggtggaatt
    10681 ggcgtcatgg caattggtac gatgttatgg gtggtactag gtaaacatat tggaattaga
    10741 gaacgtcagt taattatgtt agataataac aaaaacacaa tgagtggtac cgtcaaattg
    10801 attattgata ttgtaaaatc aatatttgta atcgaactcg taggagccat gttattagca
    10861 ttttactttt atcgagataa tccagattta aaatatgcaa tcatgcaagg tgtttttgtt
    10921 tctatttctg ccactaccaa tggtggatta gatattacag gtaagtcatt aattccttat
    10981 gcacatgatt attttgtaca agcgatagtt atatttttaa taattttagg atcaatcggc
    11041 ttcccagtat tattagaagt taaagcttat attcaaaata gggttactaa ttttagattt
    11101 tcattattta ctaaaattac gacatcaaca tatttattcc tatttattgt tggggtatta
    11161 gccattctat tatttgaaca taaccatgcg ttcaaaggtt taagttggca tcaatcgtta
    11221 ttctattcgc tgtttcaatc agcgactaca agaagtgcgg gtcttcaaac aattgatgtg
    11281 acaacactaa gtgaccccac taatattatc atgggtattt taatgtttat aggatcttcg
    11341 ccaagttcgg ttggtggcgg tattcgtaca acaactttcg ctattttaat tttgttttta
    11401 attaacttta gtaataatgc cgataaaaca tccattaaag tttacaatag agaagtacac
    11461 attatggata ttcaacgttc atttgcagta tttacaatgg cgacaatttt aacattttta
    11521 ggaatgctaa ttatatcagc tactgaaaat ggtaagctta catttttaca agtatttttt
    11581 gaagtcatgt ctgcatttgg aacttgtgga ctatcgcttg gtgtcacaag tgatattagt
    11641 gatatttcta aggtcgtact aatgatactc atgtttatag gacgtgttgg cttaatatca
    11701 tttatcatta tgatagcagg acgtcgagaa ccagataaat tccattatcc aaaagaacgt
    11761 attcaaatag gataatataa tagcaatcta agtttagtta atgtagattt taactggaac
    11821 ttagattgct tttttagttt gtatttttaa cttattttat aagacgattg gtttcgaaaa
    11881 tggtaaacta gtaacaatga gaggtgtaac atgatggaaa aaaatgaaaa cattaatgta
    11941 gagattttaa ctacgtcaga tatgcatagt catttcttaa atggtgatta tggttcaaat
    12001 atttatagag ctggtactta tgttaaccaa gtaagagcac aaaatcatcg cgtcatttta
    12061 ttagatagtg gcggaagttt agctggctcg ttagcggcct attattatgc tattgttgca
    12121 ccttataaac gacatccaat gataaagtta atgaacagaa tgcattatga tgctagcggt
    12181 gtgagtccaa gtgaattcaa gtttggttta tcatttttaa ctcgttcaat tgctttggca
    12241 cgttttccat ggttatcagc aaatattgaa tacaatgtta ctaaggagcc ttatttttca
    12301 actccatatt gtattaaaca ttttggtgac ttaaaaattg ctatcgtagg cgtcacagca
    12361 gatggtttaa tggaaaatga gtattctgaa atggagcaag atgtatctat tgaaaagaca
    12421 ttagtggcat caaaacgttg gattagatat atccatgaag ttgaagagcc agattttttg
    12481 attgtaattt atcatggtgg attgaataaa attagtaata gtacgaaaaa taaaaaggca
    12541 agttcgaatg aagctgaaaa attaatggaa gaactcggtg ttatagattt aatgattaca
    12601 gctcatcagc atcaaacaat agtaggtcaa gatcatgaaa cgtattatgt tcaggctggt
    12661 caagatgcca aagagcttgt acatctttcg attaatttta aaaagagaac aacaacttat
    12721 gatgttgaaa gcattgattc taaagtgatt gacttaaatg agtatgaaga ggatcaagaa
    12781 ttattagatt taacattcta tgatagaaaa gcagtggctt attggtcaca ggaaatcata
    12841 agtgataaag gtttgatgtt atcagtaaat gggttacaag atttagtctg tcaaacacat
    12901 ccattttcgc aattattaca tgatgcaatt caccttgcat ttgataatga tataacatgt
    12961 gtccacgtgc ctatgaacgg agagaagggg ttgagtggac agattcgaaa tgaagatttg
    13021 tatcatgcat acccatatcc agataagcca atggatatga caattagtgg tcaaaatatc
    13081 aaagatatat tggagtatag ttattcacat ttagattttg ttaacgagca attaagctta
    13141 acaattattg atgaaacgtt atgtacaatg tggcaaggat tcaattatga gattgatatg
    13201 aatcaagaac ctgggcaacg agtaatgtta gatcaaattg atttgactaa gagttataga
    13261 gttacaatga ctgactattg ttatcgtaac tacaagaatt atttaaaaaa tgctattata
    13321 catgaatcat acgatgaaac aatgagtaca ttaattgcag agaagttaag agatccgaat
    13381 tatcgtattt catgtagtga taattttata gttaaaaaca gg
//
LOCUS       DAXWFK010000066        13138 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.066, whole genome shotgun sequence.
ACCESSION   DAXWFK010000066 DAXWFK010000000
VERSION     DAXWFK010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13138
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.066"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..152
                     /locus_tag="ACGEXS_001432"
     CDS             <1..152
                     /locus_tag="ACGEXS_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HFF8012446.1"
                     /translation="ITPSQEDIITTMRLKECGLILGIDLLDHIIIGDNRFTSLVEAGY
                     FDEND"
     gene            220..504
                     /locus_tag="ACGEXS_001433"
     CDS             220..504
                     /locus_tag="ACGEXS_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012447.1"
                     /translation="MTLFIIIGVLVPMVYTMQLNIKNEPVTKRNLLITLALSTLGILV
                     TALAGVIVTKQAFPLLSVAIGSIFTGIVWGLLLSGSYALIRFLSNAFGRK"
     gene            complement(621..818)
                     /locus_tag="ACGEXS_001434"
     CDS             complement(621..818)
                     /locus_tag="ACGEXS_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012448.1"
                     /translation="MSILTIILIALLVILLFRVGLSILRFLIYVGLVLLCIYLGYQGL
                     IWLLDFFQINSGFLPHFQFNN"
     gene            978..1451
                     /locus_tag="ACGEXS_001435"
     CDS             978..1451
                     /locus_tag="ACGEXS_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4930 family protein"
                     /protein_id="HFF8012449.1"
                     /translation="MRLIFSMIKNIIAVIAIVIIVYIALKYAPFLKEQDWNPVNHDND
                     QMNQVTQPTNDAQHVYVSGEKYSLKENDLIKNVPLSQIKNVFKMIDKQEFMAVSGMNR
                     MAYNDQYIIGQRGDEFILYKFGDESMRVYNTEFEMQQDLNELGQNLQLKSENAYQ"
     gene            1525..1644
                     /locus_tag="ACGEXS_001436"
     CDS             1525..1644
                     /locus_tag="ACGEXS_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012450.1"
                     /translation="MAEQSKQKQANEQQKAQNLFARWRQLQNSNSESSNDTNK"
     gene            1844..2686
                     /gene="mreC"
                     /locus_tag="ACGEXS_001437"
     CDS             1844..2686
                     /gene="mreC"
                     /locus_tag="ACGEXS_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFF8012451.1"
                     /translation="MLKFFKNNKLIVVLCAIIVFIALIGLSIRSQSQSPPEQYIGDSV
                     SFGQRVVSYPVNFVAGTIGDFFKKGDSKESKNKISQLESKNQQLEAENEKLKKELDLK
                     DISKFDPISTTVLARNPDQWMNTIVIDKGSKSGITSNMAVMTSQGFVGRVTKVNKFSS
                     QVDLISTNTRAGKLSVNIQHGSKNIFGLIDRYDEKNSELVISDINNRDNISKGDKVVT
                     SGLADQLPSNLYIGEVTKVQNDQYGLAKEVRVKTGADLTDLSHVYVAKRDPKTIPDDE
                     SRDK"
     gene            2686..3216
                     /gene="mreD"
                     /locus_tag="ACGEXS_001438"
     CDS             2686..3216
                     /gene="mreD"
                     /locus_tag="ACGEXS_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFF8012452.1"
                     /translation="MRTLYYFLIGILLFYIDTAIGLLIPMHIGKFELVFVPHLTFMYI
                     LMMVVYRGFGVSLLLSIFLGVMTDVYFGSIYGVYLFGYILFLALIDRFFKIFYKDHSM
                     LFIIILASTLLLEVYVALIYGMLGFIQFDIIHFVVFRLLPTLIMNFVLLIMLYPLIIK
                     FLKKTNNDIDMKRRQW"
     misc_feature    3243..3370
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            3383..3691
                     /gene="rplU"
                     /locus_tag="ACGEXS_001439"
     CDS             3383..3691
                     /gene="rplU"
                     /locus_tag="ACGEXS_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFF8012453.1"
                     /translation="MFAIIETGGKQIKVEEGQEIFVEKLDVNEGDTFTFDKVLFVGGD
                     SVKVGAPTVEGATVTATVNKQGRGKKITVFTYKRRKNSKRKKGHRQPYTKLTIDKINA
                     "
     gene            3697..4017
                     /locus_tag="ACGEXS_001440"
     CDS             3697..4017
                     /locus_tag="ACGEXS_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFF8012454.1"
                     /translation="MITVDITVNDEGKVTDVIMDGHADHGEYGHDIVCAGASAVLFGS
                     VNAIIGLTSERPDINYDDNGGHFHIRSVDTNNDEAQLILQTMLVSLQTIEEEYNENIR
                     LNYK"
     gene            4029..4313
                     /gene="rpmA"
                     /locus_tag="ACGEXS_001441"
     CDS             4029..4313
                     /gene="rpmA"
                     /locus_tag="ACGEXS_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFF8012455.1"
                     /translation="MLKLNLQFFASKKGVSSTKNGRDSESKRLGAKRADGQFVTGGSI
                     LYRQRGTKIYPGENVGRGGDDTLFAKIDGVVKFERKGRDKKQVSVYAVAE"
     gene            4668..5960
                     /gene="obgE"
                     /locus_tag="ACGEXS_001442"
     CDS             4668..5960
                     /gene="obgE"
                     /locus_tag="ACGEXS_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFF8012456.1"
                     /translation="MFVDQVKISLKAGDGGNGITAYRREKYVPFGGPAGGDGGKGASV
                     VFEVDEGLRTLLDFRYQRHFKASKGENGQSSNMHGKNAEDLVLKVPPGTIIKNVETDE
                     VLADLVEDGQRAVVAKGGRGGRGNSRFATPRNPAPDFSEKGEPGEELDVYLELKLLAD
                     VGLVGFPSVGKSTLLSIVSKAKPKIGAYHFTTIKPNLGVVSTPDQRSFVMADLPGLIE
                     GASDGVGLGHQFLRHVERTKVIVHMIDMSGSEGREPIEDYKVINQELAAYEQRLEDRP
                     QIVVANKMDLPESQDNLNLFKEEIGEDVPVIPVSTITRDNIDQLLYAIADKLEEYKDV
                     DFTVEEEESVGINRVLYKHTPSQDKFTISRDDDGAYVVSGNAIERMFKMTDFNSDPAV
                     RRFARQMRSMGIDDALRERGCKNGDIVRILGGEFEFVE"
     gene            5973..6428
                     /locus_tag="ACGEXS_001443"
     CDS             5973..6428
                     /locus_tag="ACGEXS_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HFF8012457.1"
                     /translation="MDNKDYKKFYLIREDVLPESVVKTLKIKDALKSDPTLSIYDAVK
                     QFDLSRSAFYKYRETIFPVDDKMLDHREFTLILYVTDIVGMLARVLDVISKLELSVLT
                     IHQSIPMEEKATITLSLNAKSKETSVEDVIGALRNLDYVSKVELISMSM"
     gene            6442..7044
                     /gene="ruvA"
                     /locus_tag="ACGEXS_001444"
     CDS             6442..7044
                     /gene="ruvA"
                     /locus_tag="ACGEXS_001444"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFF8012458.1"
                     /translation="MYAYVKGKLTHLYPTHVVVETAGVGYEIQTPNSYRFQKHLDHEV
                     LIHTSLIVREDAQLLYGFSSEEEKDMFLSLIKVTGIGPKSALAILATSTPNEVKRAIE
                     NENDTYLTKFPGIGKKTARQIVLDLKGKVKITEEDSDSLLQVDATSTVQDQFVQEAML
                     ALEALGYSKRELAKVEKTLNKNKYDSVDEAVKAGLQLVVS"
     gene            7076..8080
                     /gene="ruvB"
                     /locus_tag="ACGEXS_001445"
     CDS             7076..8080
                     /gene="ruvB"
                     /locus_tag="ACGEXS_001445"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFF8012459.1"
                     /translation="MNERMVDQSMHSEETDFELSLRPTRLRQYIGQNSIKSNLEVFIK
                     AAKLRHEPLDHVLLFGPPGLGKTTLSNIIANEMEVNIRTVSGPSLERPGDLAAILSGL
                     QPGDVLFIDEIHRLSSVVEEVLYPAMEDFFLDIIIGKGDEARSIRIDLPPFTLVGATT
                     RAGSLTGPLRDRFGVHLRLEYYNESDLKEIIIRTAEVLGTGIDEESAIELAKRSRGTP
                     RVANRLLKRVRDFQQVNEDEQIYIETTKHALGLLQVDQHGLDYIDHKMMNCIIKQYNG
                     GPVGLDTIAVTIGEERITIEDVYEPFLIQKGFLERTPRGRKATPLAYEHFAKSNEERE
                     "
     gene            8082..9107
                     /gene="queA"
                     /locus_tag="ACGEXS_001446"
     CDS             8082..9107
                     /gene="queA"
                     /locus_tag="ACGEXS_001446"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFF8012460.1"
                     /translation="MNIEEFDYDLPESLIAQTPLKDRDHSRLLVMDRETGEMKHLHFK
                     DIIEYFRPGDTLVLNDTRVMPARLFGLKEETGAKVEMLMLTQIEGNDWEVLLKPAKRI
                     KVGNKLNFGNGKIIAECIKEMDQGGRIMRLHYEGILQERLDELGEMPLPPYIKERLDD
                     PDRYQTVYAKESGSAAAPTAGLHFTDELLIEIKNKGVNIAFVTLHVGLGTFRPVSVDD
                     VNDHEMHSEYYQMTQETADLLNDTKSKGHRIISVGTTSTRTLETIRRDHDKFVETSGW
                     TNIFIYPGFDFKAIDGQITNFHLPKSTLVMLVSAFSSRENVLNAYKTAVNLEYRFFSF
                     GDAMLII"
     gene            9130..10269
                     /gene="tgt"
                     /locus_tag="ACGEXS_001447"
     CDS             9130..10269
                     /gene="tgt"
                     /locus_tag="ACGEXS_001447"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016425271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFF8012461.1"
                     /translation="MPAVTYEHIKTCKQSGARLGIVHTPHGSFETPMFMPVGTKATVK
                     TMSPEELRQIEAKIILGNTYHLWLQPGNDIIKHAGGLHKFMNWDGPILTDSGGFQVFS
                     LSNLRKITEEGVEFRHHTNGSKLFLSPEKSMQIQNDLGSDIMMAFDECPPMPAEYDYV
                     KKSIERTTRWAKRCLDAHQRPEDQALFGIIQGGEYEDLREQSAKDLVELDFPGYAIGG
                     LSVGEPKPVMYKMVEHTEQFMPKDKPRYLMGVGSPDALIECSIRGMDMFDCVLPTRIA
                     RNGTCMTSQGRLVIKNAKFADDLRPLDENCDCYTCQNYSRAYIRHLIKAEETFGIRLT
                     TIHNLHFLLKLMEDIRQAIREDRLLDFKEEFFEQYGLNVENPKNF"
     gene            10288..10548
                     /gene="yajC"
                     /locus_tag="ACGEXS_001448"
     CDS             10288..10548
                     /gene="yajC"
                     /locus_tag="ACGEXS_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFF8012462.1"
                     /translation="MQFSLLIYIVVIFAVMYFLMIRPQQKRAKQHRELINNIQSGQRI
                     TTIGGIKGTVKAVDETTVVITVNGHGTELTFEKPAIKQVDPS"
     gene            10823..13102
                     /gene="secDF"
                     /locus_tag="ACGEXS_001449"
     CDS             10823..13102
                     /gene="secDF"
                     /locus_tag="ACGEXS_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HFF8012463.1"
                     /translation="MKKSSRIIAFLLLVVLLFAGMAATYKSVIKNVNLGLDLQGGFEV
                     LYQVDPLNKGDKIDKKALQSTAQTLENRVNVLGVSEPKIQVEEPNRIRVQLAGVTDQN
                     EARKILSSQANLTIRDAEDKVKLSGSDIKQGSAKQEFKQETNQPTVTFKVKDKNKFKK
                     VTEEISKKRDNVMVVWLDFKKGDSYKKEAQKKNPKFISAASVDQPINSDSVEISGGFK
                     GQEGVKKAKQIAELLNAGSLPVDLKEIYSNSVGAQFGQDALDKTVFASFIGVALIYLF
                     MLGFYRLPGLVAIIALTTYIYLTLVAFNFISGVLTLPGLAALVLGVGMAVDANIIMYE
                     RIKDELRIGRTIKQAFSKANKSSFLTIFDSNLTTVIAAAVLFFFGESSVKGFATMLLL
                     GILMIFVTAVFLSRFLLSLLVSSNIFKNQFWLFGVKKNKRHDINEGVDVHDLKTSFEK
                     WNFVKLAKPLIGVSILIVVVGLVILYIFKLNLGIDFSSGTRVDFQSKQAITQQKVEQV
                     VKDSGLKADQIQINGKDNKVATVQFKDDLTRAQDNKLSDNIKSKFGDTPQINTVSPII
                     GQELAKNAMLALIYASIGIIIYVSLRFEWRMGLSSVLALLHDVFIIVAIFSLFRIEVD
                     LTFIAAVLTIVGYSINDTIVTFDRVRENLQKVKVITTTEQIDDIVNRSIRQTMTRSIN
                     TVLTVIVVVVAILFFGAPTIFNFTLALFIGLISGVFSSIFIAVPLWGIMKKRQLKKSP
                     KHKLVVYKEKKSNDEKILV"
BASE COUNT         4664 a         1795 c         2540 g         4139 t
ORIGIN      
        1 acatcacgcc ctcacaagaa gatatcataa caacaatgag gttgaaggag tgtggtttga
       61 ttttagggat agatttattg gatcatatta taatcggtga taatagattt accagtcttg
      121 tagaagcggg ttactttgat gaaaatgatt gaagttgata gaattaatga cgtatcttgt
      181 gtataatacc tacgaagtac tttcattgga ggaaaaatag tgactctatt tattattatc
      241 ggggttctcg tgccaatggt ttataccatg cagttaaata ttaaaaatga acctgtaaca
      301 aagcgcaatc ttttaataac attagcttta tctacgttag gtattttagt aaccgcgtta
      361 gcaggtgtaa tcgttacgaa acaagctttt cctttattaa gtgtagcaat tggctcaatt
      421 tttactggaa tcgtttgggg ccttttacta agtggtagct acgcgctgat acgattttta
      481 tctaacgcat ttgggcgtaa gtaatagtaa tctgttcatc aagtagtatc cgtgcttgaa
      541 aacaaactaa aactcctaat gtggaactag ttaaaaaatt ctaaactagt acattaggag
      601 ttttgttatg cagaataaat ttaattgtta aattgaaagt gcggtaaaaa tccactattt
      661 atttgaaaaa aatcgagcaa ccaaattaaa ccttgatatc ctaagtaaat acataataaa
      721 acaagtccga cataaattaa aaatcgcaaa atagaaagtc caactctaaa aaggaggatg
      781 actaatagtg ctattaaaat aattgttagt atactcaatg ctacaaacct cctaatacgc
      841 ttttaaatca taacaaaatt ataagctatt ttaaatgaac gtgcatcagt cctaagatat
      901 atcatgatta aggctgaaga ctgaggaaaa tatacataat aaaatatata attagatgaa
      961 gacattggag gcgaactatg agattgatat ttagtatgat taaaaacatt atagcagtaa
     1021 tagcaattgt gataattgtt tatatcgcct tgaaatacgc acctttttta aaagaacaag
     1081 attggaatcc tgtaaatcac gataatgatc aaatgaatca agttacgcaa cccactaatg
     1141 atgcccaaca tgtttatgta agtggtgaga aatattcatt aaaagaaaat gatttaataa
     1201 agaatgtacc attaagccaa attaaaaatg tatttaaaat gatagataaa caagagttta
     1261 tggctgtgtc tggaatgaat cgcatggctt ataatgatca atatattata ggtcaaagag
     1321 gagacgaatt tattctttat aaatttggag atgagtcaat gcgtgtttac aatactgaat
     1381 ttgaaatgca acaagactta aatgaattag ggcaaaattt acaactaaaa tctgaaaatg
     1441 cttatcaata ggaaattatt gcataagaaa tatattttag ttatcctgtc actagaataa
     1501 atttaaaagg ttggtgtagt tcaaatggca gaacaatcaa aacagaaaca agctaatgaa
     1561 caacaaaagg cccagaactt attcgcacgt tggagacaac ttcagaattc gaacagtgaa
     1621 tcctcgaacg atacaaacaa ataattttaa aatttaaatc gattatttaa actactagtg
     1681 actggaatgt ttgctagtag tttttttgtt tttaaaatgc cacaaatttt ttacggagtt
     1741 gttcattttc aaagtaattc gtagttagtg ctttatttca taatgggagt aatataaaat
     1801 agaaaaggat atacaatatt ttgacataat agaggtgttc tgggtgctta agttttttaa
     1861 aaataacaaa ttaattgttg ttttatgtgc aattatcgtt tttattgcat taattgggct
     1921 gtccatacgt tcacaatctc aatcacctcc tgaacaatat ataggtgatt ctgtgtcttt
     1981 tggacaacga gttgtgagtt atccagttaa ttttgttgct ggtacgattg gggacttttt
     2041 taaaaaagga gattctaaag aatctaaaaa taagattagc cagttagaat ctaagaacca
     2101 acaattagaa gcggaaaatg aaaaattaaa aaaagagctt gatttaaaag atatttcaaa
     2161 atttgatcct atttctacta cggttttggc aagaaatccg gatcagtgga tgaatacaat
     2221 tgtaattgat aagggatcta aatcaggtat aacttcaaat atggctgtga tgacatcaca
     2281 aggttttgtt ggaagagtta ctaaagttaa taaattttct tcacaagttg atttaatctc
     2341 aactaataca cgtgcgggta aattatctgt aaatatacaa cacggttcta aaaatatatt
     2401 tggtttaatt gatcgttatg atgaaaagaa ctcagaactt gtaattagtg acattaataa
     2461 tagagataat atctcaaaag gtgataaagt cgttacaagt ggattagctg atcaactacc
     2521 aagtaattta tatataggag aagtgactaa ggttcaaaat gatcaatacg gcttagctaa
     2581 agaagttagg gttaagactg gtgcggactt aacagatttg agtcatgttt atgttgcaaa
     2641 aagagatcct aaaacaattc ctgatgatga aagcagggat aaataatgcg tacactgtat
     2701 tattttttga taggtatttt actattttat atagatactg caattgggct tcttattcca
     2761 atgcacatag gtaagtttga acttgtattt gtaccacacc ttacatttat gtatatttta
     2821 atgatggtag tgtatcgtgg ctttggcgta tcattattgc tcagcatatt tttaggcgta
     2881 atgactgatg tatactttgg tagtatttat ggagtgtact tatttggcta tatattgttt
     2941 ttagcactta tagatcgatt ctttaaaatt ttttacaaag atcattcgat gttattcatt
     3001 attatattag ccagcacctt attattagaa gtctatgtgg cattaatata cggtatgtta
     3061 ggattcattc aatttgatat tattcatttt gtagtcttta gattattgcc aacattaatt
     3121 atgaattttg tactgttgat tatgctttat ccgttgatta taaagttcct taaaaaaaca
     3181 aacaatgaca ttgacatgaa acgtcgtcaa tggtaagatg caatagttga gtagttataa
     3241 gtagctacat acaaccgctc aaatataggt tcaagaacat ttttaatgaa gaaagcgatt
     3301 ttcccacaga gtgcaaacgc ttgactcaat aacgaaatgt cacctatgta tggcgtgact
     3361 tattatacag gaggtgcaaa gtatgtttgc tattattgaa acaggtggaa aacaaatcaa
     3421 agtagaagaa ggtcaagaaa tcttcgttga aaaattagac gtaaacgaag gagatacttt
     3481 tacatttgat aaagtattat ttgtaggtgg agattcagtt aaagttggag cgccaacagt
     3541 tgaaggtgca acagttactg ctactgttaa taaacaaggt cgcggtaaaa aaatcactgt
     3601 attcacatac aaacgtcgta aaaattcaaa acgtaaaaaa ggccatcgtc aaccatacac
     3661 taaattaaca atcgataaaa tcaacgcgta attattatga ttactgttga tattacagtt
     3721 aatgatgaag gcaaagtaac agacgttatt atggatggcc atgctgacca tggtgaatat
     3781 ggtcatgata tcgtttgtgc tggagcttca gctgtattgt ttggtagtgt taatgcgatt
     3841 ataggattga catctgagag accagatatc aattatgacg acaatggtgg tcattttcat
     3901 ataagaagcg ttgatacaaa caacgatgaa gcgcaactaa ttcttcaaac aatgcttgtg
     3961 tctttacaaa ctattgaaga agaatataat gagaatatta gattaaatta taagtgaggt
     4021 gcattccgat gttaaaatta aacttacaat tcttcgcatc taaaaaaggg gtaagttcta
     4081 caaaaaacgg acgtgactct gaatcaaaac gcttaggtgc taaacgtgct gacggtcaat
     4141 tcgtaacagg tggttcaatt ttatatcgcc aacgtggtac taaaatttac cctggtgaaa
     4201 atgtaggtcg tggtggcgat gatacattat tcgctaaaat cgacggcgtt gttaaattcg
     4261 aacgtaaagg tcgcgacaaa aaacaagttt ctgtatatgc agtagctgaa taattttgtc
     4321 tagttaacac cagaagtgaa tcttctggtg ttttttactt tttataaaat gtttttcatt
     4381 attattttca ttatgttatt aaaaaatggg aattttagac gttatactaa atgtgcaact
     4441 gtataagagc ccctaatcac taaactataa gggggacaaa ggaatacagt tgcagcgttt
     4501 aaagaataaa ctgtaccaca attggtgctg agaaatataa gtattttaaa gcaaagattt
     4561 ataaaagtaa ctgcataaga gcccctaatt atttacaata tataaggggc tctaatatgc
     4621 tataattatt gggaaaatga aaattatatg taaaagaggt gagatatatg tttgtcgatc
     4681 aagtcaaaat atctcttaaa gccggtgatg gtggtaatgg tattaccgca tacagaagag
     4741 aaaaatatgt accatttggt ggaccagctg gcggtgacgg tggtaaaggt gcttcagtcg
     4801 tatttgaagt ggatgaaggt ttaagaacgt tattagattt tagatatcaa cgtcatttta
     4861 aagcaagcaa aggtgaaaat ggccaaagta gtaatatgca tggtaaaaat gcggaagatt
     4921 tagtattaaa agttccacct ggtacaatta ttaaaaatgt tgaaacagac gaagtgttag
     4981 cagatcttgt tgaagatggt caaagagctg tagtagcgaa gggcggtcga ggtggccgag
     5041 gtaattcacg ttttgcaaca cctagaaacc ctgcacctga cttcagtgaa aaaggtgaac
     5101 caggtgagga attagatgta tatttagaat tgaaattatt agctgatgta ggattagtag
     5161 gtttccctag tgtgggtaaa tcgactttat tatctatcgt ttcaaaagct aagcctaaaa
     5221 ttggggcata tcattttaca acgattaaac caaatctagg tgttgtttca acgcctgatc
     5281 aacgtagttt tgttatggca gatttaccag gtttaattga aggtgcatct gatggcgttg
     5341 gattaggaca tcaattttta agacatgtag agagaacaaa agttattgtt cacatgattg
     5401 atatgagcgg ttctgaaggt agagaaccta ttgaagatta taaagtcatt aatcaagaat
     5461 tagctgcgta cgagcaacgt ttagaagata gacctcaaat cgtagtagct aacaagatgg
     5521 atttacctga atcacaagat aatttaaact tgtttaaaga agaaattggc gaagatgtgc
     5581 cagttattcc agtttcaaca ataacgcgtg ataatattga tcaattatta tatgcaatag
     5641 cagataaatt agaagaatat aaagatgttg acttcacagt tgaagaagag gagtcagttg
     5701 gcattaaccg agtattatat aaacatacac cgtcacaaga taaatttaca atttcaagag
     5761 atgatgatgg tgcttatgtg gtaagtggta atgctattga aagaatgttt aaaatgactg
     5821 actttaacag tgatccagca gtacgtcgat ttgctcgtca aatgcgttcg atgggtattg
     5881 atgatgcgct tagagaacgt ggttgtaaaa atggtgatat cgttagaatt cttggcggag
     5941 aatttgaatt cgttgaatag gagcgaaaca tgatggacaa taaagattat aaaaagtttt
     6001 atttaattag agaagatgtc ttgcctgaat ccgtggttaa aacattgaag attaaagatg
     6061 ccttaaaaag tgatccgaca ttgtccattt atgatgccgt taaacagttt gatctatcta
     6121 gaagtgcttt ttataaatat agagaaacga tatttccagt agacgataaa atgcttgacc
     6181 atcgagaatt tacattaatt ttatatgtaa ctgatattgt tggtatgttg gcacgtgtac
     6241 tagatgttat atcaaagtta gaactatctg tattaacgat tcatcaaagt attccaatgg
     6301 aagaaaaagc aacaataaca ttatcactga atgctaaatc taaagaaact tcagtagaag
     6361 atgttattgg cgctttgaga aatttagatt atgtatcaaa agtagaatta attagtatga
     6421 gtatgtaagg atgtgcctat aatgtacgcg tatgtcaaag gtaagttaac acatttatat
     6481 cctacacacg tagttgttga aactgctggt gttggttatg aaattcaaac accaaattct
     6541 tatcgttttc aaaagcatct agatcatgaa gttttaattc atacatcttt aattgttcgt
     6601 gaagatgcac aattattgta tggatttagt agtgaagaag agaaagatat gttcttgagt
     6661 ttaattaaag ttactggtat tggtccgaaa tcagctttag ctattttagc gacaagtacg
     6721 cctaatgaag taaaacgtgc cattgaaaat gaaaatgata cgtatttaac taaattccca
     6781 ggaattggta agaaaacggc aagacagatt gtcttagatt taaaaggtaa agtgaaaatt
     6841 actgaagaag atagcgattc attattacaa gtagacgcta cttcgacggt gcaagatcaa
     6901 ttcgtgcaag aagcaatgtt agcgttagaa gcattaggtt attctaaacg agagcttgca
     6961 aaagttgaga aaacgttaaa taaaaataaa tatgactcag ttgatgaagc tgttaaggca
     7021 ggtcttcaat tagttgtatc ttaattttaa atagattaat aggggaagtg ttgtcatgaa
     7081 tgagcgtatg gttgatcaat caatgcatag tgaagaaact gatttcgaat tgtcgcttag
     7141 acctacgaga ttacgacaat atattggtca aaattcaata aaaagtaatt tagaagtatt
     7201 tattaaagcg gctaaacttc gtcatgaacc attagatcat gtattgcttt ttggcccccc
     7261 tggattaggt aagacaacat tatctaatat cattgccaat gaaatggaag ttaatatacg
     7321 tacagtatca gggccttcat tagaaagacc tggtgatttg gctgcaattt tatcaggact
     7381 tcaacctgga gatgttttgt ttattgatga aatacacaga ctgagtagtg ttgttgaaga
     7441 agtgttatac cctgcaatgg aagatttctt tttagatatt atcattggta aaggcgatga
     7501 ggctagaagt atccgtatcg acttacctcc attcactttg gtaggtgcaa caacgcgagc
     7561 tggcagctta acaggtccac taagggatcg atttggtgtg cacttaagat tagaatatta
     7621 taacgaatca gatttaaaag aaatcattat tagaacagct gaggttttag gcacaggtat
     7681 tgatgaagaa agtgccattg aacttgctaa acgttctaga gggactccaa gagtagcaaa
     7741 tcgactattg aagcgggtaa gagacttcca gcaagtgaat gaagatgaac aaatatacat
     7801 tgaaacaacg aagcacgcat taggtttact tcaagttgat caacacggac tagattacat
     7861 tgatcataaa atgatgaact gtattattaa gcagtataat ggcggacctg ttggtttaga
     7921 tacgattgcc gtaacaattg gtgaagaacg tattacaatt gaggacgttt atgagccatt
     7981 tcttattcag aaaggctttt tagaacgtac gccacgtggc agaaaagcaa caccattagc
     8041 ttatgaacat tttgcaaagt cgaatgagga gagagaataa cgtgaatatt gaagaatttg
     8101 actatgactt accagaatca ttaattgctc aaacgccttt aaaagatcgt gatcatagtc
     8161 gtttattagt catggataga gaaactggtg aaatgaaaca tttacatttc aaagatatca
     8221 ttgagtattt tagacctggt gatacattag tgcttaacga tacgcgagta atgccagcta
     8281 gactttttgg tttaaaagaa gaaactggtg caaaagttga aatgttaatg ttaactcaaa
     8341 ttgaaggtaa tgattgggaa gtcttactga aaccagctaa gcgtattaaa gttggtaata
     8401 aattgaattt tggtaatggc aaaattatag ctgaatgcat aaaagaaatg gatcaaggtg
     8461 gacgcatcat gcgtttacat tatgaaggta ttttacaaga aagattagat gaattagggg
     8521 aaatgccact gccaccatac atcaaagaac gtttagatga tccagatcgt tatcaaacag
     8581 tttacgctaa agaaagtggt tcagcggcag caccaacagc aggattacat tttactgatg
     8641 agttattaat tgaaattaaa aataaaggtg ttaatatcgc atttgttaca ttacatgttg
     8701 ggttaggtac gtttagaccg gtgagcgtag acgatgtgaa tgaccacgaa atgcatagtg
     8761 aatattatca aatgacacaa gaaacagctg atttattaaa tgatactaag tccaaaggac
     8821 atcgcattat atcagttggt acaacttcaa cacgtacact tgaaacaatt cgacgcgatc
     8881 atgataaatt tgttgaaacg agtggctgga ctaatatatt tatttatcca ggatttgatt
     8941 ttaaagcaat tgatggccag attactaatt ttcatttacc aaaatcaaca ttagttatgc
     9001 tagtatcagc gtttagtagt cgtgaaaatg ttctgaatgc ttataaaacg gcagtaaatt
     9061 tagaatatag attctttagt tttggcgatg caatgttaat tatataaaaa gaatgtgagg
     9121 attttgaata tgcctgcagt aacatacgaa cacattaaaa cttgtaaaca atcaggtgcg
     9181 cgtttaggta tcgtgcacac accacacggt tcatttgaaa cacctatgtt tatgccagtt
     9241 ggtactaaag caaccgttaa aacaatgagt ccagaagagt taagacaaat tgaagcaaaa
     9301 atcattttgg gcaacacata tcatttgtgg ttacaacccg gaaatgatat tatcaaacac
     9361 gctgggggat tacataaatt catgaattgg gatggtccga ttcttacaga ttcaggcggt
     9421 ttccaagtgt ttagtttaag taatttacgt aaaattacag aagaaggcgt ggaatttaga
     9481 catcatacta atgggtctaa attatttttg agtcctgaga aatcaatgca aattcaaaat
     9541 gatttaggat ctgatattat gatggcattt gatgaatgtc caccgatgcc tgctgaatat
     9601 gattatgtaa aaaaatctat tgaacgtaca acacgttggg cgaaaagatg tctagatgca
     9661 caccaaagac ctgaagatca agcattgttc ggcattatac aaggtggcga atatgaagat
     9721 ttaagagaac aaagtgcaaa ggatttagta gaattagatt ttcctggtta tgcaatcggc
     9781 ggtttgtcag ttggtgaacc taaaccggtt atgtataaaa tggttgaaca tacagagcag
     9841 tttatgccta aagataaacc aagatattta atgggtgtag gatctccaga tgcgttaatc
     9901 gaatgtagta ttcgcggcat ggatatgttt gattgtgtct taccgacacg tattgccaga
     9961 aatggtactt gtatgacatc gcaaggtcgt ttagttatta aaaatgcaaa atttgcagat
    10021 gatttaagac cgttagatga gaattgtgac tgttatacat gtcaaaacta ttcaagagcg
    10081 tatatacgtc atttaatcaa ggcagaggaa acttttggta ttcgtcttac tactattcat
    10141 aatttacatt ttctgctaaa attaatggaa gatataagac aagccattcg agaagatcgt
    10201 cttttagatt tcaaagaaga attcttcgag caatatggat taaatgttga gaacccaaaa
    10261 aacttttaag caagaggagc gtataaaatg caattttcat tactaatata tatagtcgta
    10321 atttttgcgg ttatgtattt cttgatgatc agaccacaac aaaaacgtgc gaaacagcat
    10381 cgtgagttga ttaataacat tcaatctggt caaagaatta caactattgg tggtattaaa
    10441 ggtactgtta aagcagtaga tgaaacaact gttgttatta cagttaatgg tcatggtact
    10501 gaattaactt tcgaaaaacc tgctattaaa caagttgacc cttcataatg agaagtggta
    10561 ttaaaaagga tgaaacgaat agcgtttcat cctttttaag ttataagtag tatgtagaat
    10621 agcttatttt tattccaaaa aaagaaattg tgatacataa taattgttaa atttaaatta
    10681 aatatatcta tgactatttt tactccctgc tctagtatgt taatatgtat aaggctaata
    10741 aattttgtaa gaaagattta acttgcgtta taaaatagta ttcaagtatt ctaaatacat
    10801 acgtaaataa tgaggtgttc atgtgaagaa aagtagtaga ataattgcgt tcttgctttt
    10861 agtcgtattg ttgtttgccg gaatggctgc aacttataaa agtgttataa agaatgtcaa
    10921 tttaggactt gatttgcaag gtggatttga ggtgctttat caagtcgatc ctttaaataa
    10981 aggcgacaaa atagataaaa aagccttaca atcaacagcg caaacattag aaaatcgtgt
    11041 taacgtttta ggtgtttcgg aacctaaaat tcaagttgaa gaacctaata gaattagagt
    11101 acaacttgct ggtgtaacag accagaatga agcacgtaaa attttatctt ctcaagctaa
    11161 tttaacaatt cgcgatgcag aagataaagt aaaattaagc ggttctgata ttaaacaggg
    11221 atctgccaaa caagaattta aacaggagac aaaccagcct acagttactt ttaaagtaaa
    11281 agacaaaaac aaatttaaaa aagtaacgga agaaatctct aagaaacgtg ataatgtcat
    11341 ggttgtttgg ttagatttca aaaaaggtga cagctataaa aaagaagcac aaaagaaaaa
    11401 ccctaaattt atttctgcag caagtgttga tcaaccaatc aattctgata gcgtagaaat
    11461 ctcgggtgga tttaaaggac aagaaggcgt taaaaaagca aaacaaatcg ccgaattgtt
    11521 aaatgcaggt tctttaccag ttgacttgaa agaaatttat tcaaactcag taggtgccca
    11581 atttggtcaa gatgcattag ataaaactgt gtttgcatcg tttattggtg tagcattaat
    11641 ttatttattc atgcttggat tctaccgatt acctggttta gtagcgatta ttgcattgac
    11701 aacttatatc tatctaacgt tagtagcatt taatttcatt tccggggttc taactttacc
    11761 aggattagcg gcgttggtat taggtgtagg tatggctgta gatgccaata ttatcatgta
    11821 tgagcgtatt aaggatgaac ttcgaatagg tagaacgata aagcaagcct tttctaaagc
    11881 aaacaaaagt tcattcctaa caatttttga ttctaactta acaacagtta tcgccgcagc
    11941 agtattattc ttcttcggtg aaagttcagt taaaggtttc gcgacaatgt tattattagg
    12001 tattctaatg atctttgtta cagccgtgtt cttatcaaga ttcttattat cattacttgt
    12061 ttcatcaaat atattcaaaa atcaattttg gttatttggt gttaaaaaga ataaacgaca
    12121 tgatattaat gaaggtgtag atgttcatga ccttaaaact tcattcgaga aatggaattt
    12181 tgttaaatta gctaagccat taattggagt aagtatttta attgtagttg tcggtttagt
    12241 tattctttat atcttcaagt taaacttagg aattgatttc tcaagtggta cacgcgtaga
    12301 tttccaatct aaacaagcaa ttactcaaca aaaggtagag caagttgtaa aagatagtgg
    12361 acttaaagcg gatcaaattc agatcaatgg taaagacaat aaagtagcaa ctgttcaatt
    12421 taaagacgat ttaacgcgtg ctcaagataa taaattgagt gataatatta aatcgaaatt
    12481 cggtgacaca cctcaaatta acactgtatc accaattatt ggacaggaat tagcaaaaaa
    12541 tgcaatgctt gcattaatct atgcatcgat aggtatcatc atctatgtat cattacgatt
    12601 tgaatggcgc atgggtcttt catctgtatt ggcattatta catgatgtat tcattatagt
    12661 agcgattttc agtttattta gaattgaagt agatttaaca tttatcgccg ctgtattaac
    12721 aattgtcggt tattcaatta atgatacaat cgtaacgttt gaccgtgtac gtgaaaactt
    12781 acaaaaggtt aaagtgatta cgacaacaga acaaattgat gatatcgtta atagatcaat
    12841 tagacagaca atgacacgtt caattaatac agtattaaca gttattgtag tagtagttgc
    12901 tatactattc ttcggtgctc ctacgatatt caactttact ttagcattat ttatcggatt
    12961 gatttctggt gtattctctt caatcttcat tgccgttccg ctatggggaa taatgaaaaa
    13021 acgtcagttg aaaaaatcgc cgaaacacaa attagttgta tataaagaaa agaaatcgaa
    13081 cgatgaaaag attttagttt aaaatgaatt aagcggtatg tgaaacaata aagagttc
//
LOCUS       DAXWFK010000067        12873 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.067, whole genome shotgun sequence.
ACCESSION   DAXWFK010000067 DAXWFK010000000
VERSION     DAXWFK010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12873
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.067"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            129..1070
                     /locus_tag="ACGEXS_001450"
     CDS             129..1070
                     /locus_tag="ACGEXS_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFF8012464.1"
                     /translation="MTKKVYFNHDGGVDDLVSLFLLLQMENVQLIGVSTIGADCYLEP
                     SLSASVKIINRFSNEDIQVAPSYERGKNPFPKEWRMHAFFMDALPILNEPVKHVASNV
                     SDKEAFEDIIQTLKRQSEKVTLLFTGPLTDLAKALQKDSSIVQYIEKLVWMGGTFLPK
                     GNVEEPEHDGSAEWNAYWDPEAVKIVFDSDIEIDMVALESTNQVPLTLDVRQRWANER
                     QYTGIDFLGVSYAAVPPLTHFITNSTYFLWDVLTTAYIGNKDLVHSIEKKVDVISYGP
                     SQGKTFECKDGRKINVINHVDNNAFFDYITALAKKVN"
     gene            1191..1745
                     /locus_tag="ACGEXS_001451"
     CDS             1191..1745
                     /locus_tag="ACGEXS_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFF8012465.1"
                     /translation="MTTKQLVYTALMTAIIAILGLVPVIPLPFSSVPIVLQNIGIFLA
                     GVILGRKYGTLSVIVFLLLVVAGLPLLSGGRGGIGVFAGPSAGFLLLYPVVAFMIGAI
                     RDRFINEINFWILFVGILVFGVIALDVIGTLIMGMIINIPFTKAISISLAYLPGDILK
                     AIVASLIGTALLNHSQFRQIMGIK"
     gene            1817..2587
                     /locus_tag="ACGEXS_001452"
     CDS             1817..2587
                     /locus_tag="ACGEXS_001452"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8012466.1"
                     /translation="MASISMSDIYCNGTIFENDDEQLIYLTPSFPQRYTSNTWIYKKT
                     PTQERWLKDLERQHQLHTNQGSNHYAFSFPENEQLDNHWMAMFKDMNFELGIMELYAI
                     ESDALANLPRNSDVEIAIVDESHIDAYLKVAYQFSLPFGKDYADAHEEMVREHYQKDV
                     IKRLVAYLNNEPIGVVDVIESENYIELDGFGVLEQFRHQGIGSTIQSLIGEYAISKNH
                     KPIILVADGEDTAKDMYAKQGYVYQSFCYQILKEDIGN"
     gene            complement(2759..3556)
                     /gene="fdhD"
                     /locus_tag="ACGEXS_001453"
     CDS             complement(2759..3556)
                     /gene="fdhD"
                     /locus_tag="ACGEXS_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFF8012467.1"
                     /translation="MNKDVSLGQPIVRYEDGKLFNTTDQYVTEFPLTIMVNGEEFATV
                     ICSPTNLEELVIGFLASEGAILKRDELKSVLIDDSKGFAHVELNKDLGDRFQYSTKRM
                     IASCCGKSREFYFQNDAAIAKTSMSKITLTPMQIINMMTRLQSASHIYQETGGLHNAA
                     ISDGLTFFVHRQDIGRHNALDKLYGFCIQRHITVRDKVLIFSGRISSEILIKAAKIGV
                     GVILSKSAPTTLAVTLANDLNITAVGFIRNGGFNIYSHPERIIDSEQ"
     gene            3846..4628
                     /gene="modA"
                     /locus_tag="ACGEXS_001454"
     CDS             3846..4628
                     /gene="modA"
                     /locus_tag="ACGEXS_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFF8012468.1"
                     /translation="MKLKRLFAVVIAMLLVLAGCSNSNDNNESKKDDADNGKKQEIQV
                     AAAASLTDVTKKLASEFKKEHKNADIKFNYGGSGALRKQIESGAPVDVFMSANTKDVD
                     ALKDKNKAHDTYKYAKNSLVLIGDKDSNYTSVKDLKDNDKLALGEVKTVPAGKYAKQY
                     LDNNNLFKEVESKIVYAKDVKQVLNYVEKGNAKQGFVYKTDLYKQNKKIDTVKVIKEV
                     ELKKPITYEAGATSDSKLAKEWMEFLKSDKAKEILKEYHFAA"
     gene            4642..5313
                     /gene="modB"
                     /locus_tag="ACGEXS_001455"
     CDS             4642..5313
                     /gene="modB"
                     /locus_tag="ACGEXS_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFF8012469.1"
                     /translation="MPDLTPFWISIRVAVISTIIVTVLGIFISKWLYRRKGSWVKVLE
                     SLLILPIVLPPTVLGFILLIIFSPRGPIGQFFANVLHLPVVFTLTGAVIASVIVSFPL
                     MYQHTVQGFRGIDTKMINTARTMGASETKIFLKLILPLAKRSILAGIMMSFARALGEF
                     GATLMVAGYIPNKTNTLPLEIYFLVEQGRENEAWLWVLVLVAFSIVVISTINLLNKDK
                     YKEVD"
     gene            5314..5919
                     /locus_tag="ACGEXS_001456"
     CDS             5314..5919
                     /locus_tag="ACGEXS_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFF8012470.1"
                     /translation="MLKINVKYQLKNTLIRINIDDTEPKIYAVRGPSGIGKTTVLNMI
                     AGLRKADEAIIEVNGQLLTDTAKNVNVKIQQRRIGYLFQDYQLFPNMTVYKNITFMAE
                     PSEHIDQLIQTLNIDHLMKQYPMTLSGGEAQRVALARALSTKPDLILLDEPFSSLDDT
                     TKDESITLVKRIFNEWQIPIIFVTHSNYEAEQMAHEIITIG"
     gene            6089..7093
                     /locus_tag="ACGEXS_001457"
     CDS             6089..7093
                     /locus_tag="ACGEXS_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HFF8012471.1"
                     /translation="MSQERYSRQILFKQIGEIGQSKINQKCALIIGMGALGTHVAEGL
                     VRAGIAKLIIVDRDYIEFSNLQRQTLFTEEDALKMMPKVVAAKKHLLALRSDVDIDDY
                     IAHVDYYFLETHGQDVDVIIDATDNFETRQLINDFAYKYRIPWIYGGVVQSTYTEAAF
                     IPGKTPCFNCLVPQLPALNLTCDTVGVIQPAVTMATSLQLRDAMKVLTEQPIDTKITY
                     GDIWEGSHYSFGFSKMQRSDCTTCGDVPSYPYLNKNEQRYATLCGRDTVQYENASITH
                     DILVQFLKQHQLNYRSNSYMVMFEFKGHRIVAFKGGRFLIHGMTRTSDATHLMNLLFG
                     "
     gene            7124..7630
                     /locus_tag="ACGEXS_001458"
     CDS             7124..7630
                     /locus_tag="ACGEXS_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFF8012472.1"
                     /translation="MGEHQNVKLNRTVKAAVLTVSDTRDFDTDKGGQCVRQLLQADDV
                     EVSDAHYTIVKDEKVAITTQVKKWLEEDIDVIITTGGTGIAQRDVTIEAVKPLLTKEI
                     EGFGELFRYLSYVEDVGTRALLSRAVAGTVNNKLIFSIPGSTGAVKLALEKLIKPELN
                     HLIHELTK"
     gene            complement(7632..8126)
                     /gene="moaC"
                     /locus_tag="ACGEXS_001459"
     CDS             complement(7632..8126)
                     /gene="moaC"
                     /locus_tag="ACGEXS_001459"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFF8012473.1"
                     /translation="MTEFTHINQQGHAKMVDVSDKQITKRTAVAHSSITVNETIFKQI
                     SNNTNTKGNVLNTAQIAGIMAAKNTSTLIPMCHPLPLTGIDVHFSWDETNAPLYTLNI
                     QTTVSTTGKTGVEMEALTAASATALTIYDMTKAVDKGMIIGETYLESKSGGKSGDFQR
                     QSNQ"
     gene            8189..9448
                     /gene="glp"
                     /locus_tag="ACGEXS_001460"
     CDS             8189..9448
                     /gene="glp"
                     /locus_tag="ACGEXS_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HFF8012474.1"
                     /translation="MVVEKRNPIPVKEAIQRIVNQQSSMPAITVALEKSLNHILAEDI
                     VATYDIPRFDKSPYDGFAIRSVDSQGASGQNRIEFKVIDHIGAGSVSDKLVGDHEAVR
                     IMTGAQIPNGADAVVMFEQTIELEDTFTIRKPFSKNENISLKGEETKTGDVVLKKGQV
                     INPGAIAVLATYGYAEVKVIKQPSVAVIATGSELLDVNDVLEDGKIRNSNGPMIRALA
                     EKLGLEVGIYKTQKDDLDSGIQVVKEAMEKHDIVITTGGVSVGDFDYLPEIYKAVKAE
                     VLFNKVAMRPGSVTTVAFVDGKYLFGLSGNPSACFTGFELFVKPAVKHMCGALEVFPQ
                     IIKATLMEDFTKANPFTRFIRAKATLTSAGATVVPSGFNKSGAVVAIAHANCMVMLPG
                     GSRGFKAGHTVDIILTESDAAEEELLL"
     gene            9445..9930
                     /gene="mobB"
                     /locus_tag="ACGEXS_001461"
     CDS             9445..9930
                     /gene="mobB"
                     /locus_tag="ACGEXS_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HFF8012475.1"
                     /translation="MILQIVGYKKSGKTTLMRHIVSFLKSHGYTVATIKHHGHGKEDI
                     QLQDSDVDHMKHFEAGADQSIVQGFQYQQTVTRVDNQNLTQIIEKSVTIDTNIVLVEG
                     FKNADFEKVVVYRNEEELQVLQQLSNVCYSINVREHEDFTAFEQWLLNKIKNDCDTQL
                     T"
     gene            9944..10390
                     /locus_tag="ACGEXS_001462"
     CDS             9944..10390
                     /locus_tag="ACGEXS_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HFF8012476.1"
                     /translation="MKQFEIVTEPIQTEQYREFTINEYQGAVVVFTGHVREWTKGVKT
                     EYLEYEAYIPMAEKKLAQIGDEINEKWPGTITSIVHRIGPLQISDIAVLIAVSSPHRK
                     DAYRANEYAIERIKEIVPIWKKEIWEDGSKWQGHQKGNYEEAKREE"
     gene            10396..10629
                     /gene="moaD"
                     /locus_tag="ACGEXS_001463"
     CDS             10396..10629
                     /gene="moaD"
                     /locus_tag="ACGEXS_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HFF8012477.1"
                     /translation="MKVLYFAEIKDILQKAQEDIVLEQALTVQQFEDLLFERYPQINN
                     KKFQVAVNEEFVQKSDFIQPNDTVALIPPVSGG"
     gene            10636..11235
                     /gene="mobA"
                     /locus_tag="ACGEXS_001464"
     CDS             10636..11235
                     /gene="mobA"
                     /locus_tag="ACGEXS_001464"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFF8012478.1"
                     /translation="MKAIILAGGHSVRFGKPKAFAEVNGETFYSRVIKTLESTNMFNE
                     IIISTNAQLATQFKYPNVVIDDENHNDKGPLAGIYTIMKQHPEEELFFVVSVDTPMIT
                     GKAVSTLYQFLVSHLIENHLDVAAFKEDGRFIPTIAFYSPNALGAITKALHSDNYSFK
                     NVYHELSTDYLDVRDVDAPSYWYKNINYQHDLDALIQKL"
     gene            11255..12277
                     /gene="moaA"
                     /locus_tag="ACGEXS_001465"
     CDS             11255..12277
                     /gene="moaA"
                     /locus_tag="ACGEXS_001465"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFF8012479.1"
                     /translation="MVEQIKDKLGRPIRDLRLSVTDRCNFRCDYCMPKEVFGDDFVFL
                     PKNELLTFDEMARIAKVYAELGVKKIRITGGEPLMRRDLDVLIAKLNQIDGIEDIGLT
                     TNGLLLKKHGQKLYDAGLRRINVSLDAIDDTLFQSINNRNIKATTILEQIDYATSIGL
                     NVKVNVVIQKGINDDQIIPMLEYFKDKHIEIRFIEFMDVGNDNGWDFSKVVTKDEMLT
                     MIEQHFEIDPVEPKYFGEVAKYYRHKDNGVQFGLITSVSQSFCSTCTRARLSSDGKFY
                     GCLFATVDGFNVKAFIRSGVTDEELKEQFKALWQIRDDRYSDERTAQTVANRQRKKIN
                     MNYIGG"
     gene            complement(12672..12842)
                     /locus_tag="ACGEXS_001466"
     CDS             complement(12672..12842)
                     /locus_tag="ACGEXS_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012480.1"
                     /translation="MFRFISFGQLVVYSTTFIPKKDILQLNGMECDINVKEPLKIYYV
                     VILKSGIEMIFS"
BASE COUNT         4503 a         1821 c         2522 g         4027 t
ORIGIN      
        1 gctttcaatt aaagaagagg tttaaaagga ttacaacaga atgttatgat tttgtagaaa
       61 gatatataac aacgttttat aaaaacataa tattgttaat ggaaaatgaa atgtaagggg
      121 gatttcgagt gactaagaaa gtttatttta accacgatgg tggtgtagat gatttagtat
      181 ctctattttt attattacaa atggaaaacg ttcaattgat aggggtcagt acaattggtg
      241 ctgattgtta tttagagcca tctttgagcg catcagtaaa aattattaat cgtttttcaa
      301 atgaagatat tcaagttgcg ccatcatatg aacgaggaaa aaatccattt cctaaagaat
      361 ggcgtatgca tgcctttttt atggacgcat tgccaatttt aaatgagcca gtcaaacatg
      421 ttgcttcaaa tgtgagcgac aaagaagcct ttgaagacat tattcaaact ttaaagagac
      481 aatcagaaaa agtaacatta ttatttacag gcccgcttac agatttagca aaagcactac
      541 aaaaagattc atctatcgtt cagtatatag aaaaattagt ttggatgggt ggcacctttt
      601 taccaaaagg aaatgttgaa gaacctgagc atgatggttc tgcagaatgg aatgcatatt
      661 gggatccaga agcggttaaa attgtttttg atagcgatat agagattgat atggttgctt
      721 tagaaagtac gaatcaagta ccgctaacgt tagatgttag acaaagatgg gcgaatgaac
      781 gccaatatac gggcattgat tttttaggag taagttatgc agcggtacca ccattaacac
      841 actttataac aaattctact tactttttat gggatgtttt aacgactgct tatattggta
      901 acaaggactt ggttcattca attgagaaaa aagtcgatgt aataagttat ggaccaagtc
      961 aaggtaagac atttgagtgt aaagatgggc gcaaaattaa tgtcataaat catgtagata
     1021 acaacgcatt ttttgattat ataactgcac ttgctaaaaa agtaaattaa cagctgtgta
     1081 gaataattaa ggttttaatt tatatagaac aacttattgt aaacttttca tttcttaaag
     1141 tttacaatgg tgctataata atggtcatga aatacgaaag gaagtaaaaa atgacaacaa
     1201 aacagttagt atatacagct ttaatgacag cgattatcgc tattttagga ttggtaccgg
     1261 taattccact accattttct tcagtaccaa ttgtacttca aaacattggt attttcttag
     1321 caggtgtgat tttaggacgt aaatatggca cattaagtgt tatcgtcttt ttattattag
     1381 tagttgctgg cttgccattg ttatcaggtg gtcgcggtgg catcggtgta ttcgcaggtc
     1441 cttcagcagg gtttttacta ttatatccag ttgtagcatt catgattggg gcgattcgag
     1501 atagattcat caatgaaatt aatttctgga ttttattcgt tggtatttta gtttttggtg
     1561 ttatagcatt agatgttatt ggtacattga ttatgggcat gattattaac ataccattta
     1621 cgaaagctat ttcaatttca ttagcttatt tgcctggtga tatattaaaa gcaattgtag
     1681 caagtttgat tggtacagct ttacttaatc actcgcagtt tcgtcaaatt atgggaataa
     1741 aataatcata tttaagatag taaagtaatt gaataagttg ctttgaaatt tataaaagtg
     1801 aaaggagtag gtgtcaatgg ctagtataag tatgtcagat atatattgta acggcactat
     1861 atttgaaaat gacgacgagc agttgattta tttaacgcct tcttttccac aacgatacac
     1921 aagtaacaca tggatatata aaaagacgcc tacccaagag cgatggctga aagacttaga
     1981 acgtcaacat caattacata caaatcaagg ttcaaatcat tatgcgttta gtttcccgga
     2041 aaatgaacaa cttgataatc attggatggc tatgtttaaa gatatgaatt ttgaactagg
     2101 tattatggaa ttgtatgcca tagaaagtga tgcgcttgcc aatttgccgc gtaactctga
     2161 cgttgaaatt gccatcgttg acgagtcgca tatagatgcc tatttaaaag ttgcatatca
     2221 gtttagtttg ccatttggaa aagactatgc agatgcacat gaagaaatgg taagggaaca
     2281 ttatcaaaaa gatgtgatta aacgcttagt agcttattta aataatgaac ctattggcgt
     2341 tgtagatgtc attgaaagtg aaaattacat tgaattagat ggatttggtg tattagaaca
     2401 atttcggcac caaggaattg gatctacaat tcaatcgttg ataggtgaat acgccatatc
     2461 aaaaaatcac aaaccaatca tattagttgc agatggtgaa gatacagcaa aagatatgta
     2521 tgcaaagcaa ggttatgtct atcaatcgtt ttgttatcaa atattaaaag aagatattgg
     2581 aaattaacga ttgatgatag gtttgtatta gaataaaaaa gcagtaagct ggtttcgagt
     2641 agaaatcaac ttactgcttt ttaaattgtt ttgagctact tatacttata aaaatagtgc
     2701 gtttaaattg ttgattcatg tagaatatcg ttcattatga cacactataa tgaatatgtt
     2761 attgttcaga atcaatgata cgttctggat gactgtatat attaaagcca ccatttcgaa
     2821 taaatccaac tgccgtaata tttaggtcat tagctaaggt tacagcaagc gttgtcggag
     2881 ctgatttaga taaaatgacg ccaacaccaa tttttgcggc tttaattaaa atttctgatg
     2941 aaatacgtcc actaaaaatt aatactttat ctcggacagt aatatgtcgc tgaatacaaa
     3001 atccatataa tttatctaga gcgttatgtc taccaatgtc ttgtcgatgt acaaaaaatg
     3061 tcaaaccatc gcttatagca gcattatgta agccacctgt ttcttggtaa atatgacttg
     3121 cactttgtaa tcgagtcatc atgttaataa tttgcattgg agttaaagtg attttagaca
     3181 tagatgtttt agcgatagca gcatcatttt gaaaataaaa ctcacgactc tttccgcaac
     3241 aagatgcaat cattcgtttt gtggaatatt gaaagcgatc gcctaaatct ttattaagtt
     3301 caacatgggc aaaaccttta ctatcatcaa tcagtacaga ttttaattca tctcgcttta
     3361 aaatggcacc ttccgaagcc agaaatccaa tgactaactc ctcaaggttt gttggactgc
     3421 atataacagt cgcaaattct tcaccattca ccataattgt aagtggaaat tctgtcacat
     3481 attgatctgt tgtattgaat aattttccat cttcatatct aacaattggt tgacctaaag
     3541 atacatcttt gttcattatc taaccccttt aattagctta aactttattt taaagcaatt
     3601 tgcttaaaat tttaacatat ttgcttaagt ttgaaatttg attgataaaa attaatagcg
     3661 agcaatctgt ttgatttaaa ttgaattcga gaatatacat actagggcat caattaataa
     3721 atatcaatct tatgcaaatt tgacaattgt ttgaatcaat atataaacag gcaacggttc
     3781 ttttcaaata taatagtaag tgtataatga aaatgtaaat attattaaaa atgggggttc
     3841 actcaatgaa attgaaacgt ttatttgctg ttgtgattgc aatgctttta gtattagctg
     3901 gttgctctaa ttctaacgat aataatgaaa gtaaaaaaga tgacgcagac aatggtaaga
     3961 aacaagagat tcaagttgca gcggcagcaa gtttaacaga tgtaaccaag aaattagctt
     4021 cagaatttaa aaaagagcat aaaaatgctg atattaaatt taactatggt ggatcagggg
     4081 cattaagaaa acaaattgaa tcaggcgcac ctgttgacgt atttatgtct gcaaatacta
     4141 aagatgtaga tgcattaaaa gacaagaata aagcgcatga tacatataaa tatgcgaaaa
     4201 atagtctagt attaattggt gataaagatt caaattacac ttcagtaaaa gacttaaaag
     4261 acaatgataa attagcatta ggtgaagtga aaactgtacc agcaggaaaa tatgcgaaac
     4321 agtatttaga taacaataac ttatttaaag aagtcgaaag taaaatcgtt tatgctaaag
     4381 atgtaaaaca agtattaaat tatgttgaaa agggtaatgc gaaacaaggt tttgtgtata
     4441 aaactgactt atataaacaa aataaaaaaa ttgatactgt aaaagtaatt aaagaagtag
     4501 aacttaagaa gccaatcaca tacgaagctg gtgctacatc agatagtaaa ttagcaaaag
     4561 agtggatgga attcttaaaa tcagataaag ctaaagaaat actaaaagaa taccactttg
     4621 cagcataagg agttgtaatc catgcctgac ttaacacctt tttggatatc aatacgagtt
     4681 gctgtaatca gtacgattat tgtaacggtt ttaggtattt ttatatctaa atggttgtat
     4741 cgtcgtaagg gttcgtgggt taaagtattg gaaagtttat tgatattacc tattgttttg
     4801 ccgccaacgg tattaggttt tattctatta atcatcttct cgccaagagg accaatcggt
     4861 caattctttg cgaatgtact acatttacct gtagtgttca ctttgacagg tgctgtgata
     4921 gcatctgtca ttgttagttt tccactaatg tatcaacata ctgtgcaagg cttcagaggt
     4981 atagacacga aaatgattaa tacagctaga acgatgggag caagtgaaac gaaaattttc
     5041 ctcaaattaa ttttaccatt agctaaacgc tctattttag caggtataat gatgagtttt
     5101 gctcgtgcat taggtgagtt tggtgctaca ttaatggttg caggatatat tccaaataaa
     5161 acgaatacac tacctttaga aatatacttc ttagtggaac aaggtagaga aaatgaagcg
     5221 tggttatggg tattagtgct agtcgcattc tctattgtgg ttatatctac aattaattta
     5281 ttgaataaag ataaatataa ggaggtcgac tagatgctta aaatcaatgt gaaatatcaa
     5341 ttaaagaaca ctttaattcg catcaatata gatgatactg aaccaaaaat ttatgcagtt
     5401 cgtggtccat ctggcattgg taaaactact gttttaaata tgattgccgg attacgtaaa
     5461 gcagatgaag ctattatcga agtgaatggg caattactta ctgatacggc aaaaaacgtg
     5521 aatgttaaaa ttcaacaacg acgtattgga tatctgtttc aagactacca attgtttcct
     5581 aatatgacgg tctataaaaa tattactttt atggctgaac catctgaaca catcgatcaa
     5641 ttaattcaaa ctttaaacat tgatcatttg atgaaacaat atcctatgac attgtcaggt
     5701 ggagaggcac aacgtgtagc acttgcacgt gcacttagca cgaaaccaga tttaatttta
     5761 ttagatgaac ctttttctag tttggatgat actacaaaag atgagagtat tacattagtt
     5821 aaacgtattt tcaacgaatg gcaaatacca atcatatttg tgacacattc aaactatgaa
     5881 gcagaacaaa tggctcatga aattattaca attgggtaat catttatttg ccattaaaga
     5941 gtttagaacg tatttaaaat tgtagaagtg aatgcttcta tcagcatttt aatgatgttt
     6001 taaactcttt tttaggggca gtttttttga gagacattga cgcgcgtcat ataatgaaag
     6061 taatgataaa aagaaaggat aacttaatgt gagtcaagaa cgttattcaa ggcaaatttt
     6121 atttaaacaa ataggtgaaa taggtcaaag caaaataaat caaaaatgtg cgttgattat
     6181 tggtatgggc gcattaggta cacatgtggc cgaaggactt gttagagcag gcattgccaa
     6241 actaatcatt gttgatagag attatattga atttagtaat ttacaaagac aaacattgtt
     6301 tactgaagaa gatgctttga aaatgatgcc taaggtggtt gcagctaaaa agcatttgct
     6361 agcgttacgt agtgatgttg atattgatga ttatattgcc catgtggatt attatttttt
     6421 ggaaacacat ggacaggacg ttgacgttat tattgatgca accgataact ttgaaacacg
     6481 acaactgatt aatgattttg catataaata tcgtatacct tggatttatg gtggtgttgt
     6541 acagagtaca tatacagaag ctgcatttat acctggtaaa acaccttgct ttaactgttt
     6601 ggtaccacaa ttgccagcat taaatttaac atgtgataca gtaggggtca ttcaacctgc
     6661 cgtgacgatg gcaacaagtt tacaattaag agatgcgatg aaagtattaa cggaacaacc
     6721 aattgacaca aaaataactt atggcgatat ttgggaaggt agtcattatt catttggttt
     6781 cagtaaaatg caacgttcag actgtacaac ttgtggagat gtaccaagtt atccgtattt
     6841 aaacaagaat gaacaacgtt atgcaacatt gtgtggtaga gacactgtac agtatgaaaa
     6901 tgcatcaatt acacacgaca ttcttgttca atttttaaaa caacatcagt taaattatcg
     6961 cagtaattcg tatatggtta tgtttgaatt taaaggacac cgcattgttg cttttaaagg
     7021 tggaaggttt ttaatacatg gcatgacacg cacatcagat gccacacatc taatgaattt
     7081 attgtttgga taaaaaaaga taagacaaaa ggagtgtaat attatgggcg aacatcaaaa
     7141 cgttaaattg aatcgtacag ttaaagcagc cgtactaacg gtatcagata ctagagactt
     7201 tgatacagat aaaggtggtc aatgcgtgcg ccaactatta caagcagatg acgttgaagt
     7261 gagtgacgca cattatacaa ttgtgaaaga tgaaaaagta gccatcacga cgcaggtgaa
     7321 gaagtggtta gaagaagata ttgatgtcat cattacgact ggtggaacag gtattgcaca
     7381 acgtgatgtg acgattgaag cagtaaaacc acttttaact aaagagatag aaggctttgg
     7441 ggaattgttt agatatttga gttatgttga agatgttggc acgcgtgcat tattgtctcg
     7501 tgctgtagca ggtacagtta ataataaatt gatattttcg attccaggat caacaggcgc
     7561 agttaaatta gcattagaaa agctcattaa accagaatta aatcatctga ttcatgagct
     7621 tacaaaataa tttattgatt tgattggcgt tgaaaatctc cagatttacc gccagacttg
     7681 ctttcaaggt aggtttcgcc aataatcata cctttatcaa ctgctttcgt catgtcgtaa
     7741 atggttaaag ccgttgctga tgcagcggtt aaagcttcca tttcaacacc ggttttgcca
     7801 gttgtagaga cagttgtttg aatgtttaaa gtataaaggg gtgcatttgt ttcatcccag
     7861 ctgaagtgaa catctatgcc agtcaatggt aatggatggc acatcggaat aagtgttgat
     7921 gtatttttgg cagccataat accagcgatt tgagcagtgt tcaatacatt gcctttggta
     7981 ttggtgttat tagaaatctg cttaaaaata gtttcattta cagtaatgct tgaatgagcg
     8041 acagcagttc tttttgtaat ttgtttgtct gatacatcga ccattttggc gtggccttgt
     8101 tgattaatat gagtaaactc agtcatttta cccctcctag tgcatctagt atatcatgaa
     8161 aaaataaaag ttttggagat gatttttaat ggtagtagaa aaaagaaacc caatcccagt
     8221 taaagaagca attcaacgta tcgttaatca gcagagttca atgccggcaa ttacggtagc
     8281 acttgaaaaa agtctaaatc atatcttagc agaagatatt gtagctactt atgatatacc
     8341 aaggtttgat aaatcacctt atgatggttt tgcaattcgc agtgttgatt cacaaggggc
     8401 aagtggtcag aatcgcattg agtttaaagt gattgatcat attggtgcag gttcagtttc
     8461 tgataaatta gttggggatc acgaagcggt gcgtattatg actggagcac aaatacctaa
     8521 tggcgcagat gctgttgtta tgtttgaaca aacgattgaa ctagaagata catttacaat
     8581 tcgtaaacca ttttcaaaaa atgaaaatat atctttaaaa ggtgaagaaa caaagacagg
     8641 cgatgttgtt ctaaaaaaag gacaagtaat taatccaggg gctatcgcgg tccttgcaac
     8701 atatggctat gcagaggtta aagttattaa gcaaccgagt gtcgctgtta ttgcaacagg
     8761 aagcgaatta ttagatgtta atgatgtatt agaagatgga aaaattcgta actctaatgg
     8821 cccaatgatt cgtgccttag cagaaaaatt aggtcttgaa gttggtattt acaaaacaca
     8881 aaaagatgat ttagatagtg gcatccaagt cgttaaagaa gctatggaaa aacatgatat
     8941 cgttattaca acgggcggag tttctgttgg agattttgac tatttacctg agatttataa
     9001 ggctgtaaag gcggaagtgt tatttaataa agtagcaatg cgtcctggta gcgtaacaac
     9061 ggttgcattt gtagatggaa agtatttgtt tggattatct ggaaatccat cagcttgttt
     9121 tacaggattt gaactatttg tgaaaccagc tgttaaacat atgtgtggcg cactagaagt
     9181 cttcccgcaa ataattaaag caacattaat ggaagatttt accaaggcaa acccattcac
     9241 acgatttata cgtgctaaag caacgttaac aagtgctgga gctactgtag taccttcagg
     9301 attcaataaa tcaggtgcgg ttgtagcgat tgcacatgct aactgtatgg tcatgttacc
     9361 aggagggtca cgtggtttta aagcggggca tacagtagat attatattga ctgaatctga
     9421 cgctgctgaa gaggaacttc ttttatgatt ttacaaattg taggttacaa aaagtctggt
     9481 aagacaacat tgatgaggca tattgtctct ttcttaaagt cacatggtta tacagttgct
     9541 actattaaac atcatgggca tggtaaggaa gatattcaat tacaggattc agacgtcgat
     9601 cacatgaagc attttgaagc gggggcagat caaagtattg tacaaggttt tcaatatcag
     9661 caaactgtaa cacgtgtaga taatcaaaat cttactcaaa ttattgaaaa atctgttaca
     9721 attgacacca atatcgtatt agttgaaggc tttaaaaatg ctgattttga aaaagtcgta
     9781 gtctatcgaa atgaagaaga gttgcaagta ttacaacaat tgtcgaatgt ttgttatagc
     9841 attaatgtaa gggagcatga agattttaca gcatttgagc aatggttatt aaataaaatt
     9901 aaaaatgatt gtgatacaca attaacatag aggattgaaa tgaatgaaac aatttgaaat
     9961 cgtgacagaa ccgatacaaa cagaacaata tcgtgaattc actataaatg aatatcaagg
    10021 tgcagtagtt gtttttaccg gtcatgttcg cgaatggact aaaggcgtca aaacggaata
    10081 tttagaatat gaagcgtata ttccaatggc tgaaaagaaa ttggcacaaa ttggagatga
    10141 aataaatgaa aaatggcctg gaacgataac gagtattgtt catagaatag ggccattaca
    10201 aatttcagat atcgctgtat taattgcggt ttcttcaccg catcgtaaag atgcctatcg
    10261 agcaaatgaa tatgcaattg agcgtataaa agaaattgtt ccgatttgga aaaaagaaat
    10321 ttgggaagat ggttcaaaat ggcaagggca tcaaaaaggg aattatgaag aagcgaagag
    10381 ggaggaataa gagagatgaa ggtactttac tttgcagaaa ttaaagatat attacaaaaa
    10441 gcacaggaag atattgtgct tgaacaagca ttgactgtac aacaatttga agatttattg
    10501 tttgaacgtt atccgcaaat caataataaa aagtttcaag ttgctgtaaa tgaggaattt
    10561 gtacaaaaat cggatttcat tcaacctaat gatactgttg cattaattcc accggttagt
    10621 ggaggttaag ggagcatgaa agcaataatt cttgcaggtg gtcattcagt gcgatttggt
    10681 aagcccaaag cttttgcgga agtgaacggt gagacctttt atagtagagt aattaagaca
    10741 ttagaatcaa caaatatgtt caatgaaatt attattagta caaatgcgca attggcaacg
    10801 caatttaaat atccaaatgt tgttatagat gatgagaatc ataatgataa aggtccatta
    10861 gcaggaattt atacaatcat gaagcaacat cctgaagaag aattgttttt tgtcgtttct
    10921 gttgatacac caatgattac tggtaaagct gtaagcacgt tgtatcagtt tttagtttct
    10981 catcttattg aaaatcattt agatgtcgca gcttttaaag aagatggacg ttttattcca
    11041 acaattgcat tttatagtcc gaatgcatta ggcgctataa ctaaagcact acattctgat
    11101 aattacagtt ttaaaaatgt atatcatgaa ttatcaacgg attatttgga tgtaagggat
    11161 gtagatgcgc cctcatattg gtacaaaaat ataaattatc agcatgattt ggacgcttta
    11221 attcaaaaat tgtaagctgt taggaggtcc acaaatggta gaacaaataa aagataaact
    11281 aggacgtccc atccgtgact tacggttatc tgtgacagat cggtgtaact ttaggtgtga
    11341 ttattgcatg cctaaagagg tatttggaga tgatttcgta tttttaccta aaaatgaact
    11401 tttaacgttt gatgaaatgg ctagaatcgc taaggtatat gcagaattag gtgtaaaaaa
    11461 aatacgcatt acaggtggag aaccattgat gcgacgggat ttagatgtac ttatagctaa
    11521 attaaatcaa atcgatggta ttgaagatat tggtttgact acaaatggtt tgttattaaa
    11581 aaagcatgga caaaagttat atgatgctgg gctgcgcaga attaatgtca gtttggatgc
    11641 tattgatgat acgctatttc aatcaatcaa taatcgtaat attaaagcga ctacgatttt
    11701 agaacaaatt gattacgcga cgtctattgg tttgaatgta aaagtaaatg ttgttataca
    11761 aaaaggtatt aacgatgatc aaatcatacc aatgcttgaa tattttaaag ataaacatat
    11821 agagattcga tttatagaat ttatggatgt tggtaatgat aatggatggg atttcagtaa
    11881 agttgtaact aaagatgaaa tgcttacaat gatagagcag cactttgaaa tcgatcctgt
    11941 agaaccaaaa tattttgggg aagtagcaaa atattatcgc cataaggata atggtgttca
    12001 atttggtttg attacaagtg tttcacaatc attttgttct acatgtacac gcgcaaggct
    12061 gtcatcagat gggaagtttt acggatgttt atttgcaact gtcgatggat ttaacgttaa
    12121 agcgtttatt cgttctggcg tgaccgacga agaattaaaa gaacaattta aagctttatg
    12181 gcaaataaga gatgatcgat attcagatga gagaactgct caaacagttg ccaatcgtca
    12241 acgtaaaaag ataaacatga attatattgg tggttaatgt gtagggacca ctacatatta
    12301 aatcattaga gatgttttaa tatttctgtc ttactcccta aaatacaata ttatttatta
    12361 aagtaaaaac ggtcatatct atgccagatt taatagaaat gatcgttttt aaagttttta
    12421 caagttggca gggccccaac acagaagctg acagaaagtc agcttacaat aatgtgcaag
    12481 ttggcggggc cccaacatag agaatttcaa aaagaaattc tacagacaat gcaagttggg
    12541 gaacggggcc ccaacacaga aggtgacgaa aagtcagcat acaataatgt gcaagttggc
    12601 ggggccccaa catagagaat ttcaaaagaa attctacaga caatgcaagt tggggatcaa
    12661 cgaaataaat tttatgagaa tatcatttct atcccactct taagaatcac tacataataa
    12721 atctttagtg gttctttaac attgatgtca cactccatgc cattgagttg taatatatct
    12781 tttttaggta taaatgttgt cgaataaaca acaagttgtc caaaagatat aaatctaaac
    12841 aagatatagc cagcaattta atatttgtaa tag
//
LOCUS       DAXWFK010000068        12736 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.068, whole genome shotgun sequence.
ACCESSION   DAXWFK010000068 DAXWFK010000000
VERSION     DAXWFK010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12736
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.068"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(66..263)
                     /locus_tag="ACGEXS_001467"
     CDS             complement(66..263)
                     /locus_tag="ACGEXS_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012481.1"
                     /translation="MNCYDEIFNTIKELIENKEISSYQINKDTGISYGNINAMRRRER
                     RIENLSLKNAKILYEYAKKVL"
     gene            complement(756..2096)
                     /gene="glnA"
                     /locus_tag="ACGEXS_001468"
     CDS             complement(756..2096)
                     /gene="glnA"
                     /locus_tag="ACGEXS_001468"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFF8012482.1"
                     /translation="MPKRTFTKDDIRKFAEEENVRYLRLQFTDILGTIKNVEVPVSQL
                     EKVLDNEMMFDGSSIEGFVRIEESDMYLHPDLDTWVIFPWTAGQGKVARLICDVYKTD
                     GTPFEGDPRANLKRVLKEMEDLGFTDFNLGPEPEFFLFKLDEKGEPTLELNDDGGYFD
                     LAPTDLGENCRRDIVLELEDMGFDIEASHHEVAPGQHEIDFKYADAVTACDNIQTFKL
                     VVKTIARKHNLHATFMPKPLFGVNGSGMHFNVSLFKGKENAFFDPNTEMGLTETAYQF
                     TAGVLKNARGFTAVCNPLVNSYKRLVPGYEAPCYIAWSGKNRSPLIRVPSSRGLSTRI
                     EVRSVDPAANPYMALAAILEAGLDGIKNKLKVPEPVNQNIYEMNREEREAVGIQDLPS
                     TLYTALKAMRENEVIKKALGNHIYNQFINSKSIEWDYYRTQVSEWERDQYMKQY"
     gene            complement(2115..2483)
                     /locus_tag="ACGEXS_001469"
     CDS             complement(2115..2483)
                     /locus_tag="ACGEXS_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFF8012483.1"
                     /translation="MISNDAIRRNMAVFSMSVVSKLTDLTPRQIRYYETHELIKPERT
                     EGQKRLFSLNDLERLLEIKSLLEKGFNIKGIKQIIYDSQEHLTTDEQEIRKKMIVDAT
                     QKPIGETLPINRGDLSRFIK"
     gene            complement(2726..3964)
                     /locus_tag="ACGEXS_001470"
     CDS             complement(2726..3964)
                     /locus_tag="ACGEXS_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFF8012484.1"
                     /translation="MKDISKIVADVESTLAPYFKEIEETAYINQEKVLNAFHHVKATE
                     SDLQGSTGYGYDDFGRDHLEEIYAQAFKAEDAIVRPQIISGTHAITIALQSLLKHGDE
                     LIYITGSPYDTLLEVIGVNGNGIESLMEHGVSYKDIALKEGKIDIESVLDGVSERTKV
                     IAIQRSKGYDQRPSIPLDEIEKVITRLKNVHPNILIFVDNCYGEFVERREPIECGADL
                     IAGSLIKNPGGGLAKIGGYIAGRKDLIERCGYRLTAPGIGKEAGASLNALLEMYQGFF
                     LAPHVVSQSLKGALFTSLFLEKMNMNTTPKYYEKRTDLIQTVKFETKEQMISFCQSIQ
                     HASPINAHFSPEPSYMPGYEDDVIMAAGTFIQGSSIELSADGPIRPPYEAYVQGGLTY
                     EHVKIAVTRAVNQLKEQGLI"
     gene            complement(3983..5221)
                     /gene="hflX"
                     /locus_tag="ACGEXS_001471"
     CDS             complement(3983..5221)
                     /gene="hflX"
                     /locus_tag="ACGEXS_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFF8012485.1"
                     /translation="MAQQQIHDTKNKLEKAVLVGVHAQDDKQFNFESTMEELSSLSET
                     CQLEVLGQITQNRDRVDRKYYVGKGKIEEIQAFIEFKDIDVVITNDELTTAQSKSLNE
                     ALGVKIIDRTQLILEIFALRARSKEGKLQVELAQLDYLLPRLQGHGKSLSRLGGGIGT
                     RGPGETKLEMDRRHIRTRMNEIKHQLRTVEEHRERYRNKRNQNQVFQVALVGYTNAGK
                     SSWFNVLANEETYEKDQLFATLDPKTRQIQINDGFNLIISDTVGFIQKLPTTLIAAFK
                     STLEEAKGADLLVHVVDSSHPEYRTQYDTVNDLIKQLDMSHISQIVIFNKKDLCDHAS
                     NRPASDLPNVFVSSKNDGDKLLVKTLFIDEIKRQLTYYDETIATNNADRLYFLKQHTL
                     VTELKYDEIENVYRIKGFKK"
     gene            5333..5808
                     /locus_tag="ACGEXS_001472"
                     /pseudo
     CDS             5333..5808
                     /locus_tag="ACGEXS_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499814.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
     gene            complement(6030..6263)
                     /gene="hfq"
                     /locus_tag="ACGEXS_001473"
     CDS             complement(6030..6263)
                     /gene="hfq"
                     /locus_tag="ACGEXS_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFF8012486.1"
                     /translation="MIANENIQDKALENFKANQTEVTVFFLNGFQMKGVIEEYDKYVV
                     SLNSQGKQHLIYKHAISTYTVETEGQASTESEE"
     gene            complement(6278..7213)
                     /gene="miaA"
                     /locus_tag="ACGEXS_001474"
     CDS             complement(6278..7213)
                     /gene="miaA"
                     /locus_tag="ACGEXS_001474"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFF8012487.1"
                     /translation="MNKNKPFIVVIVGPTASGKTELSIELAKRINGEIISGDSMQVYK
                     HMNIGTAKVTPEEMDGIPHHLIDILNPDDTFSAYEFKRLAEDLITDITNRGKVPIIAG
                     GTGLYIQSLIYNYELEDETVTPAQLSIVKQKLSALEHLDNQQLHDYLAQFDAVSAENI
                     HPNNRQRVLRAIEYYLKTKKLLSNRKKVQQFTENYDTLLLGIEMSRKTLYSRINKRVD
                     IMLDHGLFREVQQLVEQGYESCQSMQAIGYKELIPVINGQMIYEDAVNDLKQHSRQYA
                     KRQMTWFKNKMSVHWLDKENMSLQMMLDEITTQIK"
     gene            complement(7231..8145)
                     /locus_tag="ACGEXS_001475"
     CDS             complement(7231..8145)
                     /locus_tag="ACGEXS_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFF8012488.1"
                     /translation="MTEKQFKLTVQDNTNIEVKVNFTDVDSKGIIHIFHGMAEHMERY
                     DKLAHALSKHGFDVIRHNHRGHGINIDESTRGHYDDMKRVIGDAFEVAQTVRGNVDKP
                     YIIIGHSMGSVIARLFVETYPQYVDGLILSGTGMYSLWKGLPTVKVLQLITKIYGAEK
                     RVEWVNQLVSNSFNKKICPLRTQSDWISSNPIEVDNFIKDPYSGFNVSNQLLYQTAYY
                     MLHTSQLKNMKMLNHAMPILLVSGYDDPLGDYGKGILKLANIYRKAGIKNVKVNLYHH
                     KRHEVLFEKDHDKIWEDLFKWLNQFYKK"
     gene            complement(8295..9968)
                     /locus_tag="ACGEXS_001476"
     CDS             complement(8295..9968)
                     /locus_tag="ACGEXS_001476"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HFF8012489.1"
                     /translation="MALSTFKREHIKKNLRNDEYDLVIIGGGITGAGIALDASERGMK
                     VALVEMQDFAQGTSSRSTKLVHGGLRYLKQFQIGVVAETGKERAIVYENGPHVTTPEW
                     MLLPMHKGGTFGKFSTSIGLGMYDRLAGVKKSERKKMLSKKETLAKEPLVKKEGLKGG
                     GYYVEYRTDDARLTIEVMKRAAEKGAEIINYTKSEHFTYDKNQQVNGVKVIDKLTNEN
                     YTIKAKKVVNAAGPWVDDVRSGDYARNNKKLRLTKGVHVVIDQSKFPLGQAVYFDTEK
                     DGRMIFAIPREGKAYVGTTDTFYDNIKSSPLTTQEDRDYLIDAINYMFPSVNVTDEDI
                     ESTWAGIRPLIYEEGKDPSEISRKDEIWEGKSGLLTIAGGKLTGYRHMAQDIVDLVSK
                     RLKKDYGLTFSPCNTKGLAISGGDVGGSKNFDAFVEQKVDVAKGFGIDEDVARRLASK
                     YGSNVDELFNIAQTSQYHDSKLPLEIYVELVYSIQQEMVYKPNDFLVRRSGKMYFNIK
                     DVLDYKDAVIDIMADMLDYSPAQIEAYTEEVEQAIKEAQHGNNQPAVKE"
     gene            complement(10126..11622)
                     /gene="glpK"
                     /locus_tag="ACGEXS_001477"
     CDS             complement(10126..11622)
                     /gene="glpK"
                     /locus_tag="ACGEXS_001477"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFF8012490.1"
                     /translation="MEKYILSIDQGTTSSRAILFNQKGEIAGVAQREFKQYFPQSGWV
                     EHDANEIWTSVLAVMTEVINENDVRADQIAGIGITNQRETTVVWDKHTGRPIYHAIVW
                     QSRQTQSICSELKQQGYEQTFRDKTGLLLDPYFAGTKVKWILDNVEGAREKAENGDLL
                     FGTIDTWLVWKLSGKAAHITDYSNASRTLMFNIHDLEWDDELLELLTVPKNMLPEVKA
                     SSEVYGKTIDYHFYGQEVPIAGVAGDQQAALFGQACFERGDVKNTYGTGGFMLMNTGD
                     KAVKSESGLLTTIAYGIDGKVNYALEGSIFVSGSAIQWLRDGLRMINSAPQSESYATR
                     VDSTEGVYVVPAFVGLGTPYWDSEARGAIFGLTRGTEKEHFIRATLESLCYQTRDVME
                     AMSKDSGIDVQSLRVDGGAVKNNFIMQFQADIVNTSVERPEIQETTALGAAFLAGLAV
                     GFWESKDDIAKNWKLEEKFDPKMDEGEREKLYRGWKKAVEATQVFKTE"
     gene            complement(11751..12569)
                     /locus_tag="ACGEXS_001478"
     CDS             complement(11751..12569)
                     /locus_tag="ACGEXS_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFF8012491.1"
                     /translation="MNVYLAEFLGTAILILFGGGVCANVNLKRSAANGADWIVITAGW
                     GLAVTMGVFAVGQFSGAHLNPAVSLALALDGSFDWSLVPGYIVAQMLGAIVGATIVWL
                     MYLPHWKATEEAGAKLGVFSTAPAIKNYFANFLSEIIGTMALTLGILFIGVNKIADGL
                     NPLIVGALIVAIGLSLGGATGYAINPARDLGPRIAHAILPIAGKGGSNWSYAIVPILG
                     PIAGGLLGAVVYAVFYKHTFNIGCAIAIVVVIITLILGYILNKSSKKGDIESIY"
BASE COUNT         3919 a         2470 c         1773 g         4574 t
ORIGIN      
        1 taaaagcacc cactcagtca ctagtttggg cagttattgt atgcctattg aactcaatgc
       61 ttatattaca ataccttttt cgcatattca tataagatct ttgcattctt taagcttaaa
      121 ttttctattc ttctttctct acggcgcata gcattaatat taccgtaact tatcccagta
      181 tctttattaa tttgataact cgatatctct ttgttttcta tcaattcttt gattgtattg
      241 aatatttcat catagcaatt cataaattag atgaggcgaa atttttaatt ttttagaata
      301 tcaatagtag tataactaaa atgaaaatac cgatcgataa acaaaaagat attttttgtt
      361 ttgtttctct tttcatatag tattaccccc ttaataatgc gtagtaaggt ccctcttttc
      421 ggggtcttac cttagaacgt tctgcaaatg aattcgatga gaagtaatat gaatatggct
      481 attttcaagt aatactcaac gttttcgcga cgttctttta tcgcctcatc tcatcacctc
      541 caaatatatt aaaattcatg tgaactaaaa tataaaatgg tcttccccag ctttaaaaaa
      601 ataaatacat aaaacattta cttgaccaaa acttgaccaa atccattttt taacaaaagt
      661 ggtcaagtga aatcccatct ttccaaatca aaaaagagct aaagcaaaat gctctagccc
      721 ttgatattac tgatttccca tcattgttta gcgtattaat attgcttcat gtactgatct
      781 ctttcccatt cagagacttg agttctgtag taatcccatt caattgattt tgaattaata
      841 aattgattat agatatgatt tcctaaagct tttttaataa cttcattttc acgcattgct
      901 tttaatgcag tataaagtgt tgaaggtaag tcttgaatgc ctactgcttc acgttcttca
      961 cggttcattt cgtaaatatt ttggttaact ggttctggaa cttttaattt atttttaata
     1021 ccatctagtc cagcttctaa gattgcagct aacgccatgt atgggtttgc agctggatct
     1081 actgaacgta cttcgatacg agtagataat cctcttgaag atggtacacg gattaatggt
     1141 gaacggtttt taccactcca tgcaatataa catggtgctt cataaccagg tactaaacgt
     1201 ttatatgagt ttactaacgg gttacataca gcagtaaatc cgcgtgcatt tttaagcaca
     1261 cctgctgtaa attgatatgc agtttccgtt aagcccattt cagtatttgg atcaaagaat
     1321 gcattttctt tacctttgaa taatgaaacg ttaaagtgca taccgctacc attcacaccg
     1381 aataatggtt taggcataaa tgttgcgtgt aaattatgtt tacgtgcgat tgttttaaca
     1441 accaatttaa atgtttggat attatcacat gctgtaacag catctgcata tttaaagtca
     1501 atttcatgtt gaccaggggc aacttcatgg tgactagctt caatatcgaa gcccatatcc
     1561 tctaattcta aaacaatatc acgacgacag ttttcaccta aatctgtagg tgctaaatcg
     1621 aaatatccac catcatcatt aagttctaaa gttggttccc ctttttcatc caacttaaac
     1681 aagaagaatt ctggttcagg ccctaggtta aagtctgtga agcctaaatc ttccatttct
     1741 tttaatacac gttttaagtt tgcacgagga tccccttcaa atggtgttcc atctgtttta
     1801 tatacatcac aaattaaacg tgcaacttta ccttgtccag cagtccatgg gaagattacc
     1861 caagtatcta aatctggatg taagtacata tctgattctt cgatacgtac gaaaccttcg
     1921 atagaagaac cgtcaaacat catttcgtta tcaagtactt tttctaattg gcttacaggc
     1981 acttcaacat ttttaattgt tcctaaaata tcagtgaatt gtaatcttaa atatcttaca
     2041 ttttcctctt ctgcaaattt acgaatgtcg tctttagtga aagtacgttt tggcatttta
     2101 aaatcctcca aattttattt aataaatcgg gataaatcac cacgatttat tggcaaagtt
     2161 tctccaatag gcttttgcgt ggcatctaca atcatctttt ttcttatctc ttgttcatct
     2221 gttgttaaat gctcttgtga gtcataaatg atttgtttaa tccctttgat attaaatcct
     2281 ttttctaata atgatttaat ttctagtaat ctttccaaat cattgagtga gaacagacgt
     2341 ttttgacctt ctgttctttc aggtttgatg agttcatgtg tttcatagta acgtatttgc
     2401 cttggcgtta aatccgttaa cttacttact acactcatag agaagacagc catatttcgt
     2461 ctgattgcat cattcgatat cattgttcct ctcctctact tttgaacttg ttaataactt
     2521 atcataattt ttttaggatt tcaaaaatat gtcatgtttt ctaacataaa gcttaaaaca
     2581 ttgatataac tgcgatttcg acttctaaaa cgacataaaa caatctacag ctaaaatatt
     2641 ttttcaattt ttaaaaataa tctgataaat ctccgcaaaa aatttcaccc aaaacacatt
     2701 atcatttagg gtgaaattta atctattata taagtccttg ttctttcaac tgattaacag
     2761 ctcttgtcac agcaatttta acgtgttcat atgttaatcc tccttgaaca tatgcttcat
     2821 aaggaggacg aataggtcca tctgcagata attcaatcga tgaaccttga ataaacgtac
     2881 cagctgccat aataacatca tcttcgtaac caggcatata actaggttct ggactaaaat
     2941 gtgcattaat tggggatgcg tgttgaatac tttgacaaaa tgaaatcatt tgttctttcg
     3001 tttcaaattt aactgtttga attaaatcag ttcgtttttc gtagtacttc ggcgttgtgt
     3061 tcatattcat tttttctaaa aataaactag taaacaatgc acctttaaga ctctgactga
     3121 caacgtgtgg tgctaaaaag aaaccttgat acatttcaag caatgcattt aatgatgcac
     3181 ccgcttcttt accaatacca ggtgctgtca atctataacc acatcgttca attaaatctt
     3241 ttctaccagc aatgtatcca ccaatcttag ctaaaccacc gccagggttt ttaattaatg
     3301 atcctgctat taaatcagca ccacattcta taggttcacg tctttcaaca aattccccat
     3361 aacagttatc cacaaatatt aaaatattag gatgcacgtt tttcaaccta gtaattacct
     3421 tttcaatttc atctagcgga attgaaggtc tttgatcata gcctttcgaa cgttgaatcg
     3481 ctattacttt ggtgcgctca gaaaccccat ctaacacact ttcaatatcg atcttacctt
     3541 ctttaagtgc aatatcttta tacgatacgc catgctccat taaactttca ataccatttc
     3601 cgtttacgcc aatgacttca agtaaagtgt catatggact acccgttata taaattaatt
     3661 catcaccatg ttttaataaa ctttgtaatg caatagtaat cgcatgcgta cctgaaataa
     3721 tttgcggacg aacaattgca tcttctgctt taaatgcctg cgcatatatt tcttctaaat
     3781 gatcacgtcc aaagtcatca tacccgtatc ctgttgatcc ttgtagatca ctttcggttg
     3841 ctttgacatg atgaaatgca tttaatactt tttcttgatt aatatatgct gtttcttcaa
     3901 tttctttaaa atatggtgct aacgttgatt cgacgtcagc tactatctta cttatatctt
     3961 tcatttgaat ttcgtccttt tattattttt taaatccttt tatacgataa acattttcaa
     4021 tttcatcata tttaagttca gtcactaatg tatgttgttt tagaaaatat aatcgatctg
     4081 cattattcgt cgcaattgtc tcatcataat aagttaattg ccttttgatt tcatcaataa
     4141 ataacgtctt aacaagtaat ttatcaccat catttttaga agaaacaaaa acattaggca
     4201 aatcacttgc tggacgattt gatgcatgat cacataagtc ctttttatta aaaataacta
     4261 tttgagaaat atgactcata tctaattgtt tgattaaatc attaactgtg tcatactgcg
     4321 tacggtattc aggatggcta ctatctacga catgtactaa taaatctgca cctttagcct
     4381 cttctaaagt tgatttaaaa gctgcaatta acgtcgtagg tagtttctgt ataaaaccaa
     4441 cagtatctga aataattaaa ttaaatccat catttatttg aatttgtcgt gttttaggat
     4501 ctaacgttgc aaataattga tctttttcat acgtctcttc atttgctaaa acattaaacc
     4561 atgatgattt accagcattt gtataaccaa ctaaagctac ttgaaacacc tgattttgat
     4621 ttcttttatt tcgatatctt tcgcgatgtt cttctaccgt ccgcaattga tgtttaattt
     4681 cattcatacg agttcgaata tgtctgcgat ccatctctaa cttcgtttca ccagggcctc
     4741 tagttccaat accgccacct aaacgagaaa ggcttttacc atggccttgc aatctaggta
     4801 ataaataatc aagttgtgct agctctactt gcaatttacc ttctttactt cttgctctta
     4861 atgcaaatat ttcaagaatc aactgagttc tatcaataat ttttacacct aaagcttcat
     4921 ttagtgattt ggattgtgca gtcgttaatt catcatttgt gatgactaca tcaatatctt
     4981 tgaactcaat aaatgcttga atttcttcaa ttttaccttt accaacataa tatttgcgat
     5041 ctacacgatc tctgttttga gtaatttgac ccaacacttc aagttggcaa gtctctgata
     5101 aagatgataa ttcctccatt gtagactcaa aattaaattg cttatcatct tgagcatgta
     5161 caccgactaa gacagctttt tctagtttat ttttagtatc atgaatttgt tgctgagcca
     5221 tgttatacat ctccttaata aaatcctact caaagtattc tatcatagag ttttagtaaa
     5281 gactattaca cgattttgtg gtacatttta agtaaatcga aaggatgata caatggagac
     5341 aatttatgat tttgtagtag aaacaaataa aggtgttact tacaaattag atgcatataa
     5401 gggtgacgtt atgctaattg ttaatacagc tagcgaatgt ggctttactt cacaatttga
     5461 aggtttgcaa tcattatacg agaaatacaa agatcaaggg tttgtaattc taggtttccc
     5521 ttgtaatcaa tttggtggcc aagaacctgg ttcaggcgaa gaagcagctc aaaactgtaa
     5581 attaaactat ggtgttacct tccctatgca tcaaaaaata gacgtaaaag gggaacatca
     5641 attaccttta tttagatatt tgactgcggc acaacacgga ttctttaatg agaaaatcaa
     5701 atggaatttc actaagtttt tagtggatcg cgaaggtaac gttgttaaac gatttgcacc
     5761 tcaaaaaaac ctgttcaaat cgaaagagaa atagaaaaat tattataaaa tatactagct
     5821 catagcaaat aaaacgcttc atataaagtc gtatcatata ttgactcgac ctttatatga
     5881 agcgttttta ttacaaacat tcctctattg tatcttgtca tttgaacatc atttaattga
     5941 tgtcacatat gactttattt ctatatctat ttatcatact tcaaacgtta tgtacacgag
     6001 tcattaaatc gtgcccatat gattacaact tattcttcac tttcagtaga tgcttgacct
     6061 tcagtttcta ctgtataagt gctgatcgca tgtttgtaaa tcaagtgttg tttgccttga
     6121 gaatttaagc ttacgacata cttgtcgtat tcttcaataa cacctttcat ttggaaaccg
     6181 tttagaaaga atacagttac ttcagtttgg tttgctttaa aattctctag tgctttgtct
     6241 tggatgtttt cgtttgcaat catctgtcgg actcctttta cttaatctgg gttgtaatct
     6301 catctaacat catttgaagt gacatatttt ctttatctaa ccaatgaaca ctcattttat
     6361 tcttgaacca tgtcatttgt cgttttgcat attggcgtga atgttgcttt aaatcattga
     6421 cagcatcttc ataaatcatt tgtccgttaa tcacaggtat taattcttta tatccaatag
     6481 cttgcatact ttggcaagat tcatagcctt gttcaacaag ttgttgcact tctctaaata
     6541 atccgtgatc caacataata tcaacacgtt tatttattct tgaatataat gttttacgcg
     6601 acatttcaat ccctaataat aatgtatcat aattttcagt aaattgttgc actttcttgc
     6661 gattactcaa aagttttttt gtttttaaat aatattcaat agcgcgcaac actctttggc
     6721 ggttgttagg gtgaatattt tctgcagaaa ccgcatcaaa ttgagctaaa taatcgtgta
     6781 gttgctgatt atctaaatgt tctaatgcag ataacttttg tttaactatg gataattgtg
     6841 caggtgtaac tgtttcatct tctaattcat aattatatat taatgattga atatataagc
     6901 ctgttccacc tgctatgatt ggaactttac ctctattcgt tatatcagta attaaatctt
     6961 ctgctaatcg cttgaattca tatgctgaaa atgtatcatc aggattcaag atatcaatta
     7021 aatgatgtgg aataccatcc atttcttcag gtgttacttt tgcagttcca atattcatat
     7081 gtttgtagac ttgcatagag tcaccgctta tgatttcacc attgatacgc ttcgcgagtt
     7141 cgatgctaag ctctgtttta cctgaagcag ttggccccac aattactaca ataaaaggct
     7201 tatttttatt catatttaat tccactttct ttatttttta taaaattgat tcaaccattt
     7261 aaacaagtct tcccaaattt tgtcatgatc tttttcaaat aacacttcat gacgtttatg
     7321 atgataaaga ttcactttaa catttttaat gccagctttt ctatatatat tcgccaattt
     7381 taaaatccct ttaccataat cacctaaagg atcgtcatat cctgaaacta ataatatagg
     7441 catggcatga tttaacattt tcatattttt taattgtgat gtatgtagca tataataggc
     7501 tgtttgatat aataattgat ttgacacatt aaatccacta tatggatctt taataaagtt
     7561 atctacttca attggattac tagaaatcca atcactttgt gtacgtaatg gacatatttt
     7621 tttattaaaa ctatttgata ctaactggtt aacccattca actcgtttct cagcaccata
     7681 aatttttgta atcagttgta acactttaac ggttggtaaa cctttccata atgaatacat
     7741 accagtacca cttaaaatta gaccatcaac atattgcgga tatgtttcta caaacaatct
     7801 agctataacg gatcccattg aatgtccgat tataatgtat ggtttatcaa cattgcctct
     7861 cactgtttgc gctacttcaa aggcatcacc gataactcgt ttcatatcat cgtaatgccc
     7921 tcttgttgat tcatcaatat taataccatg tcctcgatga ttatgacgta tcacatcgaa
     7981 gccatgcttt gaaagtgcat gtgctaattt atcgtaacgt tccatatgtt cagccatacc
     8041 atgaaatata tgaataattc cttttgaatc tacatctgta aaattcactt taacttcaat
     8101 attcgtatta tcttgtacag ttaatttaaa ttgtttttca gtcatcatct cacaccttta
     8161 ataacaacgc ttttatattc gcttttatta ttatcataca tttagtgatg catagtttca
     8221 attgactaat gtatctcgta taaaaaaact gatgccctta aaacaggaca ccagtttatg
     8281 attgtacaaa ttaattattc tttaactgct ggttgattat ttccatgttg cgcttcttta
     8341 attgcttgct caacttcttc agtatatgct tcaatttgag ctggagagta atcaagcata
     8401 tctgccataa tatcgatgac agcatcttta taatctaata catctttaat attgaaatac
     8461 attttaccag aacgacgaac taagaaatcg ttaggtttgt ataccatttc ttgttgaata
     8521 ctataaacaa gttctacata aatttctaat ggtaacttgc tatcatggta ttgagatgtt
     8581 tgcgcaatgt tgaacaattc atcaacattt gaaccatatt tagatgctaa acgtcttgca
     8641 acatcttcat caatgccgaa tcctttagct acatctactt tttgctctac aaacgcatca
     8701 aagttcttgc taccacctac atcgccacct gaaattgcca gaccttttgt attacatgga
     8761 ctaaatgtta aaccgtagtc ttttttcaag cgtttagata ctaaatcaac aatgtcttga
     8821 gccatgtgac gatagcctgt taatttacca cctgcaatag ttaataaacc tgatttacct
     8881 tcccaaattt catccttacg agagatttca gaagggtctt tgccttcttc gtaaattaat
     8941 ggtctaattc ctgcccatgt tgattcaata tcttcatctg taacattaac actagggaac
     9001 atgtaattaa tcgcatcgat taaatagtct ctgtcttctt gtgtagttaa tggtgaagat
     9061 ttgatattgt catagaatgt atctgtagta cctacatacg cttttccttc acgtggaatt
     9121 gcaaaaatca ttcttccatc tttttcagta tcaaagtata ctgcttgacc taatgggaat
     9181 tttgattgat caataacaac atgtacacct ttagttaaac gtaatttttt attattgcgt
     9241 gcataatcac cacttctaac atcatcaacc catggacctg ctgcattaac cactttttta
     9301 gccttaattg tataattttc attagttaat ttatctataa ctttaacacc atttacttgt
     9361 tgatttttat cataagtgaa gtgttcagat ttagtatagt tgataatttc tgcgcctttt
     9421 tcagcagcac gcttcataac ttcaatagtt aaacgcgcat cgtcagtacg atattcaaca
     9481 tagtaaccgc cgccttttag accttctttt ttaactaatg gttctttagc taaagtttct
     9541 tttttagata acattttttt acgttcagac ttcttaacac ctgctaaacg atcatacatt
     9601 cctaaaccaa ttgatgttga gaatttacca aatgttccac ctttatgcat tggtaaaagc
     9661 atccactctg gagtcgtaac atgaggccca ttttcataaa caatcgcacg ttctttacca
     9721 gtttcggcaa ctactccaat ttggaattgt tttaagtaac gcaaaccacc atggactaat
     9781 tttgtagatc ttgagcttgt tccttgtgca aagtcttgca tttcaactaa tgcaactttc
     9841 attcctcttt cactcgcgtc tagtgcaata cctgcacctg taataccgcc accaataatt
     9901 actaaatcat attcatcatt tcttaaattc tttttaatat gttctctctt aaaagtagac
     9961 aatgccattc caaaacgcct cctaaaaaat gtaaaaacga gagacttgtc ccacataaac
    10021 aatccttcaa ttgtacatgt tcgaacaaat ctctcgcttc tcgattcaaa tattaactta
    10081 aattgattgt atcacaatgt ttatacaaaa gtctaatcta caagtttatt ctgttttaaa
    10141 aacttgtgtt gcttcaacag cttttttcca acctctatat aatttttctc tttcgccttc
    10201 atccattttc ggatcgaatt tttcttctaa tttccagttt ttagcgatat catctttact
    10261 ctcccagaat ccaactgcta aacctgctaa aaatgcagca cctaaagctg tagtttcttg
    10321 aatttcaggt ctttcaacag aagtattaac aatgtctgct tggaactgca taataaagtt
    10381 atttttaact gcaccaccat cgacacgtaa actttggaca tcaataccag agtcttttga
    10441 cattgcttcc ataacgtcac gagtttggta acatagtgat tctaaagttg cacggataaa
    10501 gtgctctttt tcagttccac gtgttaaacc gaaaatcgca ccacgtgctt cagaatccca
    10561 atatggtgtt cctaaaccta caaaagctgg aacaacataa acaccctcag tagagtcaac
    10621 tcgtgtcgca taactttctg attgtggtgc tgaattaatc attcttaatc catcacgtaa
    10681 ccattggatt gctgaacccg aaacaaagat ggaaccttca agcgcataat ttacttttcc
    10741 atcaatacca taagcaattg ttgttaataa accactttca gatttaaccg ctttgtcacc
    10801 tgtattcatt aacatgaagc caccagttcc atatgtgttt ttcacgtcac cacgttcgaa
    10861 gcaagcttgt ccaaataatg ctgcttgttg atcaccagct actccagcga ttggtacttc
    10921 ttgaccatag aagtggtaat caattgtctt accatatact tcactcgaag ctttaacttc
    10981 tggcaacata tttttaggta ctgtaagtag ttctaataac tcatcgtccc attctaaatc
    11041 atggatatta aacattaatg tacgactcgc atttgaataa tcagtaatat gcgcagcttt
    11101 tcctgataat ttccatacta accaagtatc aatcgttcca aataatagat cgccattttc
    11161 tgctttttct cgtgcacctt caacattgtc tagaatccat ttaacttttg tacctgcaaa
    11221 atacggatct aaaagtaatc ctgtcttatc tctaaatgtt tgttcatatc cttgttgttt
    11281 taattctgaa caaattgatt gtgtttgacg tgattgccaa acaattgcgt gataaattgg
    11341 gcggccagta tgtttgtccc aaacaaccgt tgtttcacgt tggtttgtaa taccgatacc
    11401 tgcaatttga tcagctctaa catcattttc attaattact tccgtcatta cagctaacac
    11461 agatgtccaa atttcatttg catcatgttc aacccaacct gattgtggaa aatattgctt
    11521 aaactcacgt tgtgctaccc ctgcaatttc ccctttttga ttgaataaaa tcgctcttga
    11581 gcttgttgtt ccttggtcta tagataaaat atatttttcc attagtagta tctcctttat
    11641 taaaaaataa tcgctaaccc attgtacaaa atctgttaaa ttttggcgaa aaaatagcat
    11701 aatccatgaa tcaaacatgt taaattatgc tatttacgtt tctttttatt ttagtaaatt
    11761 gattcgatat cacctttttt tgatgattta tttaaaatgt aacctaaaat caaagtaata
    11821 ataactacaa caattgcaat tgcacaacca atattaaatg tatgtttata aaatacagcg
    11881 tataccactg cacctaataa accaccggca attggtccta agataggaac gattgcatat
    11941 gaccaatttg aaccaccttt accagctatt ggtaaaatcg catgtgcaat tctcggacct
    12001 aaatcacgtg ctgggttgat tgcataacca gtagcaccgc ctaaacttaa tccgattgca
    12061 acaattaatg ctccgacaat taaaggattt aaaccatcgg caattttgtt tacaccgata
    12121 aataaaatac ctaaagttaa tgccattgtt ccgataatct cacttaaaaa gttggcaaag
    12181 taattcttaa tagccggtgc tgtagagaaa acacctaatt tcgcgccagc ttcttctgtc
    12241 gctttccaat gtggcaagta cattaaccat acaattgttg ctccgacaat tgcacctaac
    12301 atttgagcaa caatataacc aggaactaat gaccaatcaa aacttccgtc taatgcaaga
    12361 gctaaagaca ccgctgggtt taaatgtgca cctgagaatt gaccgacagc aaacacaccc
    12421 attgtaaccg ctaatcccca tccagctgtg atgacaatcc aatcagcacc attcgcagca
    12481 cttctcttta aattgacatt ggcacaaacg ccaccaccaa aaaggattaa gattgcagtt
    12541 cctaggaatt ctgctaaata tacattcata ttaaatgtcc tcccaaaaag acaaagagat
    12601 tcctacacta gcagtttggc tcgtagaaat ctcccgtctc tattctatgt attaactttt
    12661 attatatttg ttatgatact atcgatatga aagcgttgtc aatggttttt gtaaaaattt
    12721 tgtcaaattt aatttt
//
LOCUS       DAXWFK010000069        12606 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.069, whole genome shotgun sequence.
ACCESSION   DAXWFK010000069 DAXWFK010000000
VERSION     DAXWFK010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12606
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.069"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            56..2152
                     /gene="pnp"
                     /locus_tag="ACGEXS_001479"
     CDS             56..2152
                     /gene="pnp"
                     /locus_tag="ACGEXS_001479"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFF8012492.1"
                     /translation="MSQEKKVFKTEWAGRSLTIETGQLAKQANGAVLVRYGDTVVLST
                     ATASKEPRDGDFFPLTVNYEEKMYAAGKIPGGFKKREGRPGDDATLTARLIDRPIRPL
                     FPKGYKHDVQIMNMVLSADPDCSPQMAAMIGSSMALSVSDIPFQGPIAGVNVGYIDGK
                     YIINPTVEEKEVSRLDLEVAGHKDAVNMVEAGASEITEQEMLEAIFFGHEEIQRLVDF
                     QQQIVDHIQPVKQEFIPAERDEALVERVKSLTEEKGLKETVLTFDKQQRDENLDNLKE
                     EIVNEFIDEEDPENELLIKEVYAILNELVKEEVRRLIADEKIRPDGRKPDEIRPLDSE
                     VGILPRTHGSGLFTRGQTQALSVLTLGALGDYQLIDGLGPEEEKRFMHHYNFPNFSVG
                     ETGPVRAPGRREIGHGALGERALKYIIPDTADFPYTIRIVSEVLESNGSSSQASICGS
                     TLALMDAGVPIKAPVAGIAMGLVTREDSYTILTDIQGMEDALGDMDFKVAGTKEGITA
                     IQMDIKIDGLTREIIEEALEQARRGRLEIMNHMLQTIDQPRTELSAYAPKVVTMTIKP
                     DKIRDVIGPGGKKINEIIDETGVKLDIEQDGTIFIGAVDQAMINRAREIIEEITREAE
                     VGQTYQATVKRIEKYGAFVGLFPGKDALLHISQISKNRIEKVEDVLKIGDTIEVKITE
                     IDKQGRVNASHRALEE"
     gene            2388..4061
                     /locus_tag="ACGEXS_001480"
     CDS             2388..4061
                     /locus_tag="ACGEXS_001480"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFF8012493.1"
                     /translation="MSLIKKKNKDIRIIPLGGVGEIAKNMYIVEVDDEMFMLDAGLMF
                     PEDEMLGIDIVIPDISYVLENKDKLKGIFLTHGHEHAIGAVSYVLEQLDAPVYGSKLT
                     IALIKENMKARNIDKKVRYYTVNNDSIMRFKNVNISFFNTTHSIPDSLGVCIHTSYGA
                     IVYTGEFKFDQSLHGHYAPDIKRMAEIGEEGVFVLISDSTEAEKPGYNTPENVIEHHM
                     YDAFAKVRGRLIVSCYASNFIRIQQVLNIASKLNRKVSFLGRSLESSFNIARKMGYFD
                     IPKDLLIPITEVDNYPKNEVIIIATGMQGEPVEALSQMAQHKHKIMNIEEGDSVFLAI
                     TASANMEVIIANTLNELVRAGAHIIPNNKKIHASSHGCMEELKMMINIMKPEYFIPVQ
                     GEFKMQIAHAKLAAEAGVAPEKIFLVEKGDVINYNGKDMILNEKVNSGNILIDGIGIG
                     DVGNIVLRDRHLLAEDGIFIAVVTLDPKNRRIAAGPEIQSRGFVYVRESEDLLREAEE
                     KVREIVEAGLQEKRIEWSEIKQNMRDQISKLLFESTKRRPMIIPVISEI"
     gene            4317..6686
                     /locus_tag="ACGEXS_001481"
     CDS             4317..6686
                     /locus_tag="ACGEXS_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFF8012494.1"
                     /translation="MAQAKKKSTAKKKTTSKKRTNSRKKKNDNPIRYVIAILVVVLMV
                     LGVFQLGIIGRLIDSFFNYLFGYSRYLTYILVLLATGFITYSKRIPKTRRTAGSIVLQ
                     IALLFVSQLVFHFNSGIKAEREPVLSYVYQSYQHSHFPNFGGGVLGFYLLELSVPLIS
                     LFGVCIITILLLCSSVILLTNHQHREVAKVALENIKAWFGSFNEKMSERNQEKQLKRE
                     EKARLKEEQKARQNEQPQIKDVSDFTEVPQERDIPIYGHTENESKSQSQPSRKKRVFD
                     AENSSNNIVNHHQADQQEQLTEQTHNSVESENTIEEAGEVTNVSYVVPPLTLLNQPAK
                     QKATSKAEVQRKGQVLENTLKDFGVNAKVTQIKIGPAVTQYEIQPAQGVKVSKIVNLH
                     NDIALALAAKDVRIEAPIPGRSAVGIEVPNEKISLVSLKEVLDEKFPSNNKLEVGLGR
                     DISGDPITVPLNEMPHLLVAGSTGSGKSVCINGIITSILLNAKPHEVKLMLIDPKMVE
                     LNVYNGIPHLLIPVVTNPHKAAQALEKIVAEMERRYDLFQHSSTRNIKGYNELIRKQN
                     QELDEKQPELPYIVVIVDELADLMMVAGKEVENAIQRITQMARAAGIHLIVATQRPSV
                     DVITGIIKNNIPSRIAFAVSSQTDSRTIIGTGGAEKLLGKGDMLYVGNGDSSQTRIQG
                     AFLSDQEVQDVVNYVVEQQQANYVKEMEPDAPVDKSEMKSEDALYDEAYLFVVEQQKA
                     STSLLQRQFRIGYNRASRLMDDLERNQVIGPQKGSKPRQVLIDLNNDEV"
     gene            6691..7404
                     /locus_tag="ACGEXS_001482"
     CDS             6691..7404
                     /locus_tag="ACGEXS_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8012495.1"
                     /translation="MSEMNAVYNVKQYILNLIKQNKLEYGDQLPSNLSIARELNVKTD
                     DVYEAIQALITEQVIKDNFEEGTSVKSLPPFFYPLNELISIGQMIKNAGFECGTEYLN
                     FDEQPATMLDANLLSVEEGYPVTIIERLRTADGEPVVYCLDKIAKKELTCTEYQMSNG
                     SILSAIKEQSNHNICYADTEIEAVNYEPRISEVLNASPHEGLILLKITHYNESDEPIL
                     YSLNYMKNSLVQFKITRKI"
     gene            7435..8700
                     /locus_tag="ACGEXS_001483"
     CDS             7435..8700
                     /locus_tag="ACGEXS_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFF8012496.1"
                     /translation="MSRQSQPNIHIKVSPTTKFKTTTIVFKFMAPLEYDTITARSLLS
                     KLLVRATKKWPTDKSFNNHLADLYGAYVNSTISKFKDQHVITFSLEIVNERYLRNGES
                     LFNQGLDLLQEIIWNPLIENKAFNDNFVNQEKTLLAKKIEAMVDNKAQYSFLKLLDHM
                     FENEAYKYLSTGQLEQIPHITAETLYHTYQSMINNDQCSVYVVGNVEPESVEKQIREK
                     FALKPFDKHQFQHSTHHLHDEEVDYIVEYDDVDQAKLNMGYRFPTQYGQSGYAAFVVF
                     NMMFGGDPSSVLFNEVREKQSLAYSIHSQIDGKNGYLFVLSGVSSDKYETAKDTIISE
                     FEKIKAGDFTEEKLELAKKVIISHRYESEDRPKSIIEIMHNQILLEQPQSKETFINDI
                     QKVIREDIVSVAEKAFLDTIYVLTKGGDK"
     gene            8700..9986
                     /locus_tag="ACGEXS_001484"
     CDS             8700..9986
                     /locus_tag="ACGEXS_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFF8012497.1"
                     /translation="MKERYYELIDERVFEQELENGLRLFIIPKPGFQKTFVTYTTQFG
                     SLDNQFKPLGQDQFVTVPDGVAHFLEHKLFEKEEEDLFTAFAEDNAQANAFTSFDRTS
                     YLFSATDNIENNIKRLLTMVETPYFTKETVDKEKGIIAEEIKMYQEQPGYKLMFNTLR
                     AMYQQHPIRVDIAGSVESIYDITKDDLYLCYETFYHPSNMVLFVVGDVDPEAICRIVK
                     QHEDARNKVNQPKIERGLVDEPEDVKEAFVTESMKIQSPRLMLGFKNKPLQEAPQKYV
                     QRDLEMSLFFELIFGEETDFYQNLLNEGLIDDTFGYQFVLEPTYSFSIVTSATEEPDK
                     LKKLLLDELRDKKGNFQDAEAFELLKKQFIGEFISSLNSPEYIANQYTKLYFEGVSVF
                     DMLDIVENITLDSINETSSLYLNLDQQVDSRLEIKK"
     gene            9986..10690
                     /locus_tag="ACGEXS_001485"
     CDS             9986..10690
                     /locus_tag="ACGEXS_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFF8012498.1"
                     /translation="MKALVLGGSGSIGSEIVKQLLTDGFEVYVQYYRTDINELTSKFN
                     DDKVRFIQADLSQTIDIDKTFGDIKSLDCLIYASGQSLYGVLQDMKDHDIDACYQLNV
                     LQLIRLCRYFVDVLRQSANGRIIVISSIWGETGASMETIYSAMKSAQLGFVKALSQEL
                     ALTSVTVNAIAPGFVAGNMASEWQEDELQAMITELPQQRLVLPSEVAHTCAYLYHPNA
                     RSVTGTIQKVNGAWYI"
     gene            10795..11622
                     /locus_tag="ACGEXS_001486"
     CDS             10795..11622
                     /locus_tag="ACGEXS_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfK family protein"
                     /protein_id="HFF8012499.1"
                     /translation="MTVAEKKEWYLEYEIEINRPGLLGDVSSLLGMLGISIVTINGVD
                     QGKRGLLIKTDNLEKVERFEQIARGINEIEITKLKKPELRDRLAVRHGRYIEQDAKDK
                     KTFRFEREDLGLLVDFLAELFKEEGHKLIGIRGMPRVGKTESIVAGSVCAHKRWLFIS
                     STLIKQTVRSSLIKGEYDANHVYIIDGAVTARESNPKHQELVNEVMTLPSIKVVEHPD
                     LFVETSTCTMEDFDYIIELRENENQEIHYEEMKKQTVQSKNNLDFGDPFGGGFGFFE"
     gene            11641..12033
                     /locus_tag="ACGEXS_001487"
     CDS             11641..12033
                     /locus_tag="ACGEXS_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8012500.1"
                     /translation="MKTVGEALKGRRERLGMTLTELEQRTGIKREMLVHIENNEFDQL
                     PNKNYSEGFIRKYASVVNIEPNQLIQAHQDEIPSNQAEWDEVITVFNNNKDLDYKSKS
                     KEPIQLLVIMGITVLITLLLWIMLVLIF"
     gene            12067..>12606
                     /gene="pgsA"
                     /locus_tag="ACGEXS_001488"
     CDS             12067..>12606
                     /gene="pgsA"
                     /locus_tag="ACGEXS_001488"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFF8012501.1"
                     /translation="MNIPNQITVFRVVLIPVFILFALVDFGFGNVSFLGGYEIRIELL
                     ISGFIFILASLSDFVDGYLARKWNLVTNMGKFLDPLADKLLVASALIVLVQLGLTNSV
                     VAIIIIAREFAVTGLRLLQIEQGFVSAAGQLGKIKTAVTMVAITWLLLGDPLATLIGL
                     SLGQILLYIGVIFTILSGIE"
BASE COUNT         4587 a         1695 c         2452 g         3872 t
ORIGIN      
        1 aatacgataa taaatgatat gatataacta ttagattcaa gagaggagat ttataatgtc
       61 tcaagaaaag aaagttttta aaactgaatg ggcaggaaga tctttaacga ttgaaacagg
      121 gcaattagct aaacaagcaa atggcgctgt attggttcgt tatggagata cagtcgtgtt
      181 atcgacggca actgcatcaa aagaacctcg tgatggagat ttcttcccat taacagtgaa
      241 ctatgaagaa aaaatgtacg ctgcgggtaa aattcctggt ggatttaaaa agagagaagg
      301 acgtcctggt gacgatgcaa cattaactgc gcgattaatt gatagaccaa ttagaccttt
      361 attccctaaa ggatataagc atgatgttca aattatgaac atggtattaa gtgcagatcc
      421 tgattgttca ccacaaatgg ctgcaatgat tggttcatct atggcgctta gtgtgtcgga
      481 tattccattc caagggccaa tcgccggtgt aaatgtgggt tatattgacg gtaaatatat
      541 cattaaccca acagtagaag aaaaagaagt ttctcgttta gaccttgaag tagctggtca
      601 taaagatgcg gtaaacatgg tagaggcagg cgctagtgag attactgaac aagaaatgtt
      661 agaggcgatt ttctttggtc atgaagagat tcaacgttta gttgatttcc aacaacaaat
      721 cgtcgaccac attcaacctg ttaaacaaga atttattcca gcagagcgtg atgaagcgct
      781 agttgaacgt gtaaaatctt taaccgaaga aaaaggactt aaagaaacag ttttaacatt
      841 tgataaacaa caacgagatg aaaatcttga taacttaaaa gaagaaatcg tcaatgaatt
      901 tatcgatgaa gaagatccag agaatgaatt acttattaaa gaagtttatg caattttaaa
      961 tgaattagtg aaagaagaag ttcgacgttt aattgcagat gaaaaaatta gaccagacgg
     1021 ccgtaaacct gatgaaatcc gtccattaga ttctgaagtt ggtattttac ctagaacgca
     1081 tggttcaggt ctatttacac gtggtcagac tcaagcactt tcagttttaa cattaggtgc
     1141 tttaggcgat tatcaattaa ttgatggttt aggacctgaa gaagaaaaaa gattcatgca
     1201 tcattacaac ttcccgaatt tttcagtagg tgaaactggt ccagtacgtg cgccaggtcg
     1261 tcgtgaaatt ggacatggtg cgttaggtga aagagcatta aaatatatta ttcctgatac
     1321 tgctgatttc ccatatacaa ttcgtattgt aagtgaggta cttgaatcaa atggttcatc
     1381 atctcaagcg tcaatttgtg gatcaacatt agcattaatg gatgcgggcg taccgattaa
     1441 agcaccagtt gctggtattg ctatgggcct tgttacacgt gaagatagct atacgatttt
     1501 aactgatatc caaggtatgg aagatgcatt aggtgatatg gactttaaag tcgctggtac
     1561 taaagaaggt attacagcaa tccaaatgga tattaaaatt gacggtttaa cgcgtgaaat
     1621 tatcgaagag gctctagaac aagcgagacg tggtcgttta gaaataatga atcatatgtt
     1681 acaaacaatt gatcaaccac gtactgaatt aagtgcttac gcgccaaaag ttgtaactat
     1741 gacaattaaa ccagataaga ttagagatgt tatcggacct ggtggtaaaa aaattaacga
     1801 aattattgat gaaacaggtg ttaaattaga tattgaacaa gatggtacta tctttattgg
     1861 tgctgttgat caagctatga taaatcgtgc tcgtgaaatc attgaggaaa ttacacgtga
     1921 agcggaagta ggtcaaactt atcaagccac tgttaaacgt attgaaaaat acggtgcgtt
     1981 tgtaggccta ttcccaggta aagatgcgtt gcttcacatt tcacaaattt caaaaaatag
     2041 aattgaaaaa gtggaagatg tattaaaaat cggtgacaca attgaagtta agattactga
     2101 aattgataaa caaggtcgag taaatgcttc acatagagca ttagaagaat aatatttaaa
     2161 gtcatatgac gacaatgtat cgtcatgtga tttttttatg ccacttttta cgaagtgacc
     2221 cgttttgaat ttgttgtatt gaacatttta aaacgcttta ttattttgtg tgcaactgtt
     2281 aattatcctg tatgtatagt gattaatagt gtacatcaag tgttttttaa cttataatga
     2341 atagtgagtt tatatatgga cgggtaacaa atttaggagg taagattttg agtttaataa
     2401 agaaaaagaa taaagatatt cgcattatac cattaggcgg tgttggcgaa attgctaaaa
     2461 atatgtatat cgttgaagta gacgatgaaa tgtttatgtt agatgctgga cttatgtttc
     2521 cagaagacga aatgctaggt attgatattg ttataccaga catttcatac gtacttgaaa
     2581 ataaagataa attgaagggt atattcctta cacacggaca tgagcacgcg attggtgcag
     2641 tgagttatgt tttagaacaa ttagatgcac cagtatatgg atctaaattg acaatagcgt
     2701 taattaaaga aaatatgaaa gcccgtaata ttgataaaaa agttcgctac tatacagtta
     2761 ataatgattc aattatgaga ttcaaaaacg tgaatattag tttctttaat acgacacaca
     2821 gtattcctga tagtttaggt gtttgtattc acacttcata tggtgccatt gtgtatacag
     2881 gtgaatttaa gtttgaccaa agtttacatg gacattatgc accagatatt aaacgtatgg
     2941 cagagattgg tgaagaaggc gtatttgtct taatcagtga ttctactgag gcagagaaac
     3001 ctggatataa tactccggaa aatgtgattg aacatcatat gtatgatgct tttgcaaaag
     3061 tgcgaggtcg cttgatagtt tcatgttatg cttcgaactt tatacgtatt cagcaagttt
     3121 taaatattgc tagcaagcta aatcgtaaag tgtcattttt aggaagatca cttgaaagtt
     3181 catttaatat tgctcgtaaa atggggtatt tcgacattcc taaagatttg ctaattccta
     3241 taacagaagt tgataattat cctaaaaatg aagtgataat tatagctact ggtatgcaag
     3301 gagaacctgt agaagcctta agtcaaatgg cgcaacataa gcataaaatt atgaatatcg
     3361 aagaaggcga ttctgtattt ttagcaatta cggcttctgc taatatggaa gttatcattg
     3421 cgaatacatt aaatgagctt gtacgtgctg gcgcacatat tattccaaat aacaaaaaga
     3481 ttcatgcttc aagtcatggt tgcatggaag aattaaaaat gatgattaat attatgaaac
     3541 ctgaatactt tattcctgta caaggtgaat ttaaaatgca gatagcacat gcgaagctag
     3601 cagctgaagc aggtgttgca ccagaaaaga ttttccttgt ggaaaaagga gatgtcatta
     3661 attacaacgg taaagatatg atattaaatg aaaaggtaaa ttcaggaaat attttaatag
     3721 atggcattgg tattggggat gtaggaaata tcgtgttgag agaccgtcat cttttagcag
     3781 aagatggtat ctttattgct gttgtaacgt tagatcctaa aaatagacgt atagctgcgg
     3841 gacctgaaat tcaatctcgt gggtttgtat atgtacgtga aagtgaagac ttattacgtg
     3901 aagcagaaga gaaagtacgt gaaatagtag aggctggttt acaagaaaaa cgcatagaat
     3961 ggtctgaaat taaacaaaat atgcgtgatc aaattagtaa actattattc gaaagtacaa
     4021 aacgtcgtcc tatgattatt ccagtaattt ctgaaattta atcaaaaagt cattaacata
     4081 aaagaggtca gaacaagtca ctgaaatata atggttgtca tggacaattt acttatattt
     4141 tatgatggtc aattgaaggg gtaacgatta atctgttatc ttaagtaaat tgatacatag
     4201 atgatattgt tctaacctct ttcatcgtct gtttggacta catattctaa acatcaaata
     4261 ggaaattata tataataacg tcgttttaac taaggcaaca taaggaggtg cgtcaattgg
     4321 cacaagcaaa aaagaaatcg acagctaaga aaaaaacaac atcaaaaaaa agaacaaatt
     4381 cgaggaaaaa gaagaatgat aatccgatac gttatgtcat agctatttta gtagttgtat
     4441 taatggtgtt gggtgttttc caattaggaa taataggtcg tctaattgac agcttcttta
     4501 attatttatt tgggtacagt agatatttaa catatatttt agtactctta gcaactggtt
     4561 ttattacata ctctaaacgt attcctaaaa ctagacgaac ggctggttcg attgtattgc
     4621 aaattgcatt gctatttgta tcacagttag tttttcattt taatagtggt atcaaagctg
     4681 aaagagaacc tgtactttct tatgtgtatc agtcatacca acacagtcat ttcccaaatt
     4741 ttggtggcgg tgtattaggc ttttatttat tagagttaag cgtaccttta atttcattat
     4801 ttggtgtatg tattattact attttattat tatgctcaag tgttatttta ttaacaaacc
     4861 atcaacatcg tgaagttgca aaagttgcac tggaaaatat aaaagcttgg tttggttcat
     4921 ttaatgaaaa aatgtcggaa agaaaccaag aaaaacaatt gaagcgtgaa gaaaaagcaa
     4981 gacttaaaga agaacaaaag gcacgtcaaa atgaacagcc acaaataaaa gatgtgagtg
     5041 attttacgga agtgcctcaa gaaagagata ttccaattta tgggcatact gaaaatgaaa
     5101 gtaaaagcca gagtcaacca agtcgaaaaa aacgagtgtt tgatgcagag aatagttcga
     5161 ataacatcgt aaatcatcat caagcagatc agcaagaaca attaacagaa caaactcata
     5221 acagtgttga aagtgaaaac actattgaag aagctggtga agttacgaat gtatcgtatg
     5281 ttgttccacc gttaacttta cttaatcaac ctgcaaaaca aaaagcaaca tctaaagctg
     5341 aagtgcaacg taaaggacaa gtactagaga atacattaaa agattttggg gtaaatgcaa
     5401 aagtgacaca aattaaaatt ggtcctgcag taactcaata tgaaattcaa ccagctcaag
     5461 gggttaaagt gagtaaaatt gtaaacttgc ataatgatat tgcattagct ttagcagcaa
     5521 aagatgttag aatcgaagcg ccaatacctg gtcgttctgc agtaggtatt gaagtgccaa
     5581 atgagaaaat ttcattagtt tcactaaaag aagttttaga tgaaaaattc ccgtctaata
     5641 ataaactaga agttggatta ggaagagata tatcaggtga tccaattact gttccactaa
     5701 atgaaatgcc acacttattg gtggcaggat cgacgggtag tggtaaatct gtttgtataa
     5761 atggtattat tacaagtatt ttattaaatg ctaagccgca tgaagttaaa cttatgttaa
     5821 tcgatccgaa aatggttgaa ctaaatgttt ataacggaat tccacactta ttaattccgg
     5881 ttgttacaaa tcctcataaa gctgctcaag ctttagaaaa aattgtagct gagatggaaa
     5941 gacgttatga tttattccaa cattcatcaa ctagaaacat taaaggttat aacgaattaa
     6001 tccgtaagca aaatcaagaa ttagatgaga agcaaccaga attaccttat atcgttgtta
     6061 ttgtagatga gcttgcagat ttaatgatgg tagctggtaa agaagttgaa aatgcgattc
     6121 aacgtattac acaaatggca cgtgcagcag gtatacattt aattgtagcg acacaaagac
     6181 cttctgtgga tgtaattaca ggtatcatta aaaataatat tccatctaga atagcttttg
     6241 ctgtgagttc tcaaacagat tcaagaacta ttattggtac tggcggcgca gaaaagttac
     6301 ttggtaaagg tgacatgtta tacgttggaa atggtgactc atcacaaaca cgtattcaag
     6361 gggcgttttt aagtgaccaa gaggtgcaag atgttgtaaa ttatgtagta gaacaacaac
     6421 aggcaaatta tgtaaaagaa atggaaccag atgcaccagt ggataaatcg gaaatgaaaa
     6481 gtgaagatgc tttatatgat gaagcgtatt tgtttgttgt tgaacaacaa aaggcaagta
     6541 catcattgtt acaacgccaa tttagaattg gttataatag agcatctagg ttgatggatg
     6601 atttagaacg caatcaggta atcggtccac aaaaaggaag caagcctaga caagttttaa
     6661 tagatcttaa taatgacgag gtgtaaaaaa atgtcagaaa tgaatgcggt atataacgtt
     6721 aaacaataca ttttaaattt gattaagcaa aataaattgg aatatggtga ccaacttcca
     6781 agtaatttat caattgccag agaattaaat gtaaaaaccg acgatgttta tgaagcaatt
     6841 caggcattga ttactgaaca agtcattaaa gataattttg aagagggcac aagtgttaag
     6901 tcactgcccc ctttctttta tccattgaat gaacttataa gtattgggca aatgattaaa
     6961 aatgcaggat ttgaatgcgg aactgaatac ttaaattttg atgagcaacc agcaactatg
     7021 ttagatgcaa atttgttgag cgttgaagaa ggatatccag taaccattat agaacgatta
     7081 cgaactgccg atggagaacc ggtcgtctat tgtttagata aaattgctaa aaaagaatta
     7141 acatgtacag agtatcaaat gagcaatgga tcgattctaa gtgcgataaa agaacaaagt
     7201 aatcataata tttgttatgc tgatacagaa attgaagcgg taaattatga acctcgaata
     7261 tccgaagtac tgaatgcttc gccacacgaa ggtttgattt tattaaaaat tacgcactat
     7321 aatgaatcag atgaacctat tttgtattca ttaaattata tgaaaaatag cttagttcaa
     7381 tttaaaatca ctagaaaaat atagatgtaa gaagatatag aggaggctat atatttgagt
     7441 agacaatctc aaccaaatat acatatcaaa gtttcaccaa caactaaatt taaaacaact
     7501 actatagttt ttaaatttat ggcaccttta gaatatgaca caataacagc tagatcatta
     7561 ttaagcaaat tattagttcg agcaactaag aaatggccaa ccgataagtc gtttaataat
     7621 cacttagccg atttatatgg tgcgtatgtg aatagtacaa tttcaaaatt caaagatcag
     7681 catgtcatta cattttcatt agaaattgtt aatgaacgtt atttaagaaa cggtgaatca
     7741 ttatttaatc aaggattaga tttattacaa gaaatcattt ggaatccatt aattgaaaat
     7801 aaagcattca atgataattt tgttaaccaa gagaaaacat tattagccaa aaaaatagaa
     7861 gcaatggtag ataataaagc acaatattcg tttttaaaat tactcgacca tatgtttgaa
     7921 aatgaagcat ataaatactt atctacagga caactagaac aaatcccaca tattactgct
     7981 gaaacactat atcatacata tcaatcaatg attaataatg atcaatgttc tgtttatgtt
     8041 gtcggcaatg tagaacctga aagtgttgag aaacaaatac gtgaaaaatt tgcacttaaa
     8101 ccattcgata aacatcaatt ccaacattct actcatcatt tacacgatga agaagttgat
     8161 tatattgttg aatatgatga cgtggatcaa gctaaattaa atatgggata ccgttttcca
     8221 acacaatatg gacaaagtgg atatgctgcc tttgttgtat ttaacatgat gtttggagga
     8281 gatccttcat ctgttttatt taatgaagtg cgagaaaagc aaagtttagc gtactctata
     8341 cattcacaaa ttgatggcaa aaatggctat ttatttgttt tgagtggggt ttcaagtgat
     8401 aagtacgaaa ctgcaaaaga cactattata agtgaatttg aaaaaataaa agcaggagat
     8461 ttcactgaag aaaaattaga gttagctaaa aaagtaatca tttctcatcg atatgaatct
     8521 gaagatcgtc cgaaaagtat tatagagatt atgcataacc aaatattatt agagcaacca
     8581 caaagcaaag aaacatttat aaatgatata cagaaggtaa ttcgcgaaga tattgtttct
     8641 gttgctgaaa aagcattttt agatacaatc tatgtgttga caaaaggagg ggataaataa
     8701 tgaaagagcg ttattatgaa ttaatagacg aaagagtatt cgaacaagaa ttagaaaatg
     8761 gtttacgatt atttattatc cccaaaccag gttttcaaaa gacatttgtc acttacacta
     8821 cacaatttgg ttcattagat aatcaattca aaccccttgg acaagaccaa tttgttactg
     8881 tacctgatgg agttgctcac tttttagaac ataaattatt tgaaaaagaa gaagaagact
     8941 tatttactgc gtttgctgaa gataacgcac aagcaaatgc gtttacaagc tttgatcgta
     9001 caagctactt gttcagtgca actgataata ttgaaaacaa cattaaacgt ttacttacaa
     9061 tggttgaaac gccttatttt acaaaagaaa ctgttgataa agaaaaaggt attattgcag
     9121 aagaaataaa aatgtatcaa gagcaacctg gatataaatt aatgtttaat acattgcgtg
     9181 caatgtatca acaacatcca atacgtgttg atattgccgg tagtgtagaa agtatatacg
     9241 atattacaaa agatgatttg tatctatgtt atgaaacgtt ttatcatcca tcaaatatgg
     9301 ttttatttgt tgttggcgat gtggatcctg aagcaatatg tcgaatagta aaacaacacg
     9361 aggatgctcg taataaagtt aaccaaccca aaatcgaacg aggacttgtt gatgaaccgg
     9421 aggatgttaa agaagcattt gttactgaat ctatgaaaat tcaatcacca agactaatgc
     9481 ttggttttaa aaataaacca ttacaagaag cgcctcaaaa atatgtacaa cgtgatttag
     9541 aaatgtcatt attctttgag ttaatttttg gggaagaaac agatttttat cagaatttat
     9601 taaacgaagg acttatcgat gatacatttg gttatcaatt tgtactagag ccgacgtata
     9661 gtttttcaat cgtgacaagt gctactgaag aaccagataa attaaaaaaa ttattattag
     9721 atgagttgcg tgataaaaaa ggcaatttcc aagatgcaga agcatttgaa cttttgaaaa
     9781 agcaatttat aggtgaattc atatcaagtt taaactcacc tgaatatatt gctaatcaat
     9841 atactaaatt gtattttgaa ggtgttagcg tgtttgacat gttagacatt gttgaaaata
     9901 tcacgttaga tagtattaac gaaacgtcat cgttatattt aaatttagat cagcaagtcg
     9961 atagtcgttt ggagattaaa aagtaatgaa agcattagta ttaggtggtt ctggttcaat
    10021 tggttctgag atagtcaaac aattattaac tgatggattt gaagtttatg tgcaatatta
    10081 tcgtactgat ataaatgaat taactagcaa atttaatgat gataaagttc gttttataca
    10141 agcggattta tctcaaacaa ttgatattga caaaacattt ggtgacatta aatcattaga
    10201 ctgtttaata tatgcaagtg gtcagtcttt atatggtgtt ttacaagata tgaaagacca
    10261 tgatattgat gcatgttatc agttaaatgt cttgcaatta attcgattat gtagatattt
    10321 cgttgatgtt ttacgtcaaa gtgccaatgg aagaattatt gtaatttcat caatttgggg
    10381 tgagacagga gctagtatgg aaactattta ttcggcgatg aaaagtgcac aattaggttt
    10441 cgttaaggcg cttagtcaag agcttgcact aacatcagtg acagtaaatg ctatcgcacc
    10501 tggatttgta gccggtaata tggcaagtga gtggcaagaa gatgaacttc aagcaatgat
    10561 aactgaatta ccacaacagc gattggtttt accgagtgag gttgctcata catgcgccta
    10621 tttatatcac ccaaatgcta gaagtgttac tggaactata cagaaagtta atggtgcttg
    10681 gtatatttaa aatatagaac tatttttatg agcaaatgtt tttatgattg atgttataat
    10741 atgaatatta caatgaatca taatttgtta attaaacaag gggtgaggtt caccatgaca
    10801 gttgcagaga aaaaagaatg gtacctagaa tacgaaattg aaattaatag accgggtctt
    10861 ttaggtgatg tatctagttt attaggtatg ttaggtataa gtattgttac aattaatggt
    10921 gttgatcaag gtaaacgagg ccttttaatt aaaacagaca atcttgaaaa agttgaacgt
    10981 tttgagcaaa tagctcgtgg tataaatgaa attgaaataa caaagcttaa aaaaccagaa
    11041 ttaagagacc gtcttgcagt aagacatgga agatatattg agcaagatgc aaaagataag
    11101 aaaacctttc gatttgagcg tgaagattta ggcttgttag tagacttttt agctgaattg
    11161 ttcaaagaag aaggtcataa gttgattggc attagaggta tgccacgggt tggtaaaact
    11221 gaatcaattg ttgcgggaag tgtttgtgca cataagagat ggttatttat tagttctact
    11281 ttaataaaac aaactgtacg tagctctcta attaaagggg aatatgatgc caatcatgta
    11341 tacattattg atggtgcagt tactgccaga gaatctaatc caaaacatca agagcttgtt
    11401 aacgaagtta tgacgttacc atcaatcaaa gtcgttgaac atccagattt atttgttgaa
    11461 acaagtactt gtacaatgga agattttgac tatattatcg aattgagaga aaatgaaaat
    11521 caagaaatac attacgaaga aatgaagaaa cagacagtcc aaagtaagaa taatttagat
    11581 tttggagatc cgtttggtgg tggttttggt ttcttcgagt aagtgtaagg aggctatgaa
    11641 ttgaaaacgg tcggtgaagc gctaaaaggt agacgtgaaa ggttaggaat gactttaaca
    11701 gaattagagc aacgtactgg aattaaacgt gaaatgctag tgcatattga aaataatgaa
    11761 ttcgatcaac taccgaataa aaattacagc gaaggattta ttagaaaata tgcaagcgta
    11821 gtaaatattg aacctaacca attaattcaa gctcatcaag atgaaattcc atcgaaccaa
    11881 gccgaatggg acgaagtaat tacagttttc aataataata aagacttaga ttataagagt
    11941 aaatcaaaag agccaataca attattagta atcatgggta ttacagtttt aataacttta
    12001 ttgttatgga tcatgttagt tttaatattt taacagaaat aaattagtga gaaatgagga
    12061 tgtataatga atattccgaa ccagattacg gtttttagag tagtgttaat accagttttt
    12121 atattgtttg cgttagttga ttttggattt ggcaatgtgt catttctagg aggatatgaa
    12181 ataagaattg agttattaat cagtggtttt atttttatat tggcttccct tagcgatttt
    12241 gttgatggtt atttagctag aaaatggaat ttagttacaa atatggggaa atttttggat
    12301 ccattagcgg ataaattatt agttgcaagt gctttaattg tacttgtgca actaggacta
    12361 acaaattctg tagtagcaat cattattatt gccagagaat ttgccgtaac tggtttacgt
    12421 ttactacaaa ttgaacaagg attcgtaagt gcagctggtc aattaggtaa aattaaaaca
    12481 gcagttacta tggtagcaat tacttggttg ttattaggtg atccattggc aacattgatt
    12541 ggtttgtcat taggacaaat tttattatac attggcgtta tttttactat cttatctggt
    12601 attgaa
//
LOCUS       DAXWFK010000070        12575 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.070, whole genome shotgun sequence.
ACCESSION   DAXWFK010000070 DAXWFK010000000
VERSION     DAXWFK010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12575
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.070"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            110..1027
                     /locus_tag="ACGEXS_001489"
     CDS             110..1027
                     /locus_tag="ACGEXS_001489"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFF8012502.1"
                     /translation="MENKYTHGVLFYHEHSGLKNINQGIGEVTTALSSICKHLSIQLS
                     ENEGDIIKYCQEIKTKNYAKDVDILFILGGDGTVNELINGVMTHDLQLPIGILPGGTF
                     NDFTKTLNIAPNHKQASEQMISAQVGTYDVIKINNQYALNFVGLGLIVQNAENVQDGS
                     KDIFGKLSYIGSTVKTLLNPTQFNYQLSIDDKTYSGETTMILTANGPFIGGSRIPLTD
                     LSPQDGELNTFIFNEQSFSILNDIFKKRDSMNWNEITQGIEHIPGKKISLTTDPAMKV
                     DIDGEISLETPIDIEVIPNAIQLLTVNDL"
     gene            complement(1650..2192)
                     /locus_tag="ACGEXS_001490"
     CDS             complement(1650..2192)
                     /locus_tag="ACGEXS_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'(3')-deoxyribonucleotidase"
                     /protein_id="HFF8012503.1"
                     /translation="MTRKSIAIDMDEVLADTLGEIIDAVNFRADLGIKMEALNGQKLK
                     HVIPEHDGLITEVLREPGFFRHLKVMPYAQEVVKKLTEHYDVYIATAAMDVPTSFSDK
                     YEWLLEFFPFLDPQHFVFCGRKNIVKADYLIDDNPRQLEIFTGTPIMFTAVHNINDDR
                     FERVNSWKDVEQYFLDNIEK"
     gene            complement(2543..3601)
                     /gene="hisC"
                     /locus_tag="ACGEXS_001491"
     CDS             complement(2543..3601)
                     /gene="hisC"
                     /locus_tag="ACGEXS_001491"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFF8012504.1"
                     /translation="MKEQLNQLSAYQPGLSPRALKEKYGIEGDLYKLASNENLYGPSP
                     KVKEAISAHLDELYYYPETGSPTLKAAISKHLNVDQSRILFGAGLDEVILMISRAVLT
                     PGDTIVTSEATFGQYYHNAIVESANVIQVPLKDGGFDLEGILKEVNEDMSLVWLCNPN
                     NPTGTYFNHESLDSFLSQVPPHVPVIIDEAYFEFVTAEDYPDTLALQQKYDNAFLLRT
                     FSKAYGLAGLRVGYVVASEHAIEKWNIIRPPFNVTRISEYAAVAALEDQQYLKEVTHK
                     NSVERERFYQLPQSEYFLPSQTNFIFVKTKRVNELYEALLNVGCITRPFPTGVRITIG
                     FKEQNDKMLEVLSNFKYE"
     gene            complement(3841..5355)
                     /locus_tag="ACGEXS_001492"
     CDS             complement(3841..5355)
                     /locus_tag="ACGEXS_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFF8012505.1"
                     /translation="MTNFFDILSERKGQLFSTMIEHIQISFIALLIATAIAVPLGILL
                     TKTKTISEIVMNIAAILQTIPSLALLGLMIPLFGIGRVPAIIALVVYALLPILRNTYT
                     GIKEVDPSLIEAAKGIGMKPFRRLTKVELPIAMPVIMAGVRTAMVLIIGTATLAALIG
                     AGGLGDLILLGIDRNNASLILLGAIPAALLAIIFDLILRFMAKLSYKKLLMTLGVIVM
                     IIILAIAIPMFAQKGDKITLAGKLGSEPSIITNMYKILIEEETKNTVEVKDGMGKTAF
                     LFNALKSDDIDGYLEFTGTVLGELTKEPLKSKEEKKVYEQAKQSLEKKYQMTMLKPMK
                     YNNTYALAVKRDFAKQHNIRTIGDLNKVKDQLKPGFTLEFNDRPDGYKAVQKAYNLNL
                     DNIRTMEPKLRYQAINKGNINLIDAYSTDAELKQYDMVVLKDDKHVFPPYQGAPLFKE
                     SFLKKHPEIKKPLNKLENKISDEDMQMMNYKVTVKNEDPYTVAKDYLKAKGLIK"
     gene            complement(5348..6325)
                     /locus_tag="ACGEXS_001493"
     CDS             complement(5348..6325)
                     /locus_tag="ACGEXS_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8012506.1"
                     /translation="MIKFKNVTKRYGKHVAVDNISFNINEGEFFVLIGPSGCGKTTTL
                     KMINRLIHLSEGYIYFKDKPISDYPVYEMRWDIGYVLQQIALFPHMTIKENIAQVPQM
                     KKWKEKDIDKRVDELLEMVGLEPEKYKNRKPDELSGGQRQRVGVIRALAADPPVILMD
                     EPFSALDPISREKLQDDLIELQTKIKKTIIFVTHDIQEAMKLGDKICLLNEGHIEQID
                     TPEGFKNNPQSEFVKQFMGSHLEDDAPCVEENAIIRDLDIMKPIDEVTSMSAYPIVYD
                     NQPIEVLYQLLSESERVIVMQEDSVGQYVIDRKDIFKYLSQKKEVAQHD"
     gene            complement(6547..8328)
                     /gene="recQ"
                     /locus_tag="ACGEXS_001494"
     CDS             complement(6547..8328)
                     /gene="recQ"
                     /locus_tag="ACGEXS_001494"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFF8012507.1"
                     /translation="MMQQTLSHYFGYETFRPGQEEIISKVLDHRNVLGVLPTGGGKSI
                     CYQVPGLLLGGTTIVISPLISLMKDQVDQLKAMGIQAAFLNSSLTQKEQQRIEKALSN
                     GEIQFLYVAPERFENRYFLNMLQRIKIHLVAFDEAHCISKWGHDFRPSYQNVISKVFT
                     LPQDFTIIALTATATVEVQQDIREKLNIAQTDQIKTSTKRRNLIFKVNPTYQRQKFIL
                     DYIKTHDEDAGIIYCSTRKQVEELQEALESQKIESVIYHAGLSNKEREEAQNDFLFDR
                     VKVVVATNAFGMGIDKSNVRFVIHYNMPGDLESYYQEAGRAGRDGLKSECILLFSERD
                     INLHEYFITVSQADDDYKDKMGEKLTKMIQYTKTKKCLEATIVHYFEPNEKLEECEQC
                     SNCVQQDKSYNMTQEAKMIISCIARMKQQESYSVIIQVLRGESTDYIKYKGYDQISTH
                     GLMKGYTTSELSHLIDELRFKGFLNENDEILMCDTSIKKLLSNEVEVFTTPFKQKATE
                     KVFINTVEGVDRVLFSQLVEVRKKLSDKLTIAPVSIFSDYTLEEFAKRKPASKQDMIN
                     IDGVGSYKLKHYCPAFLETIQNYKAKV"
     gene            complement(8340..10223)
                     /locus_tag="ACGEXS_001495"
     CDS             complement(8340..10223)
                     /locus_tag="ACGEXS_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFF8012508.1"
                     /translation="MGMEAYKIEHLNKSYADKTIFDNLDLSISEGEKIGLVGINGTGK
                     STLLKVIGGIDDDFTANVMHPNQYRIRYSSQKQDLNEDMTVFDAVLSSDTTTLRIIKQ
                     YEQAVQAYADDQSDKLFKRMMDAQDAMDQHDAWDYNAEIKTILSKLGIHDTTKYIKEL
                     SGGQQKRVVLAKTLIEQPDLLLLDEPTNHLDFESISWLINYVKQYPHTVLFVTHDRYF
                     LNEVSTRIIELNRGKLASYPGNYESYIEMRAEREVTLQKQQQKQRALYKEELAWMRAG
                     AKARTTKQQARINRFNDLENEVNQQYKDDKGELNLAYSRLGKQVFELEDLSKAINDKV
                     LFEHLTEIIQKGERIGVVGPNGAGKTTLLNILSGEDQQFEGKLKTGQTVKVAYFKQTD
                     ETLDRDIRMIDYLREESEIAKEKDGTSVSITQLLERFLFPSATHGKKVYKLSGGEQKR
                     LYLLRLLVHQPNVLLLDEPTNDLDTETLTILEDYIHTFGGTVITVSHDRYFLNKVAQS
                     YWFIHDGQMEKIIGTFEDYESYKKSLDKNKSTLKQQSKSSTTVRKKNGLSYKEKLEYE
                     QLMKRIEQAEVRMEEIDVLMIEASADYGKIKELNEEKEQLEIQYDLDITRWSELEEIK
                     EQQ"
     gene            complement(10494..12434)
                     /gene="ltaS"
                     /locus_tag="ACGEXS_001496"
     CDS             complement(10494..12434)
                     /gene="ltaS"
                     /locus_tag="ACGEXS_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyglycerol-phosphate lipoteichoic acid
                     synthase LtaS"
                     /protein_id="HFF8012509.1"
                     /translation="MSSQKKKISLFAFFLLTVITITLKTYFSYYVDFSLGVKGLVQNL
                     ILLMNPYSLVALVLSVFLFFKGKKAFWFMFIGGFLLTFLLYANVVYFRFFSDFLTFST
                     LNQVGNVESMGGAVSASFKWYDFVYFIDTLVYLFILIFKTKWLDTKAFSKKFVPVVMA
                     ASVALFFLNLAFAETDRPELLTRTFDHKYLVKYLGPYNFTVYDGVKTIENNQQKALAS
                     EDDLTKVLNYTKQRQTEPNPEYYGVAKKKNIIKIHLESFQTFLINKKVNGKEVTPFLN
                     KLSSGKEQFTYFPNFFHQTGQGKTSDSEFTMDNSLYGLPQGSAFSLKGDNTYQSLPAI
                     LDQKQGYKSDVMHGDYKTFWNRDQVYKHFGIDKFYDATYYDMSDKNVVNLGLKDKIFF
                     KDSANYQAKMKSPFYSHLITLTNHYPFTLDEKDATIEKSNTGDATVDGYIQTARYLDE
                     ALEEYINDLKKKGLYDNSVIMIYGDHYGISENHNNAMEKLLGEKITPAKFTDLNRTGF
                     WIKIPGKSGGINNEYAGQVDVMPTILHLAGIDTKNYLMFGTDLFSKGHNQVVPFRNGD
                     FITKDYKYVNGKIYSNKNNELITTQPADFEKNKKQVEKDLEMSDNVLNGDLFRFYKNP
                     DFKKVNPSKYKYETGPKANSKK"
BASE COUNT         3956 a         2259 c         1759 g         4601 t
ORIGIN      
        1 caatttcatt tattaccatt ttctctattt aaaaacatat ttttggtatt aacaattaaa
       61 agtgggtata tatatttaat gaaccaattt ttagaaagta ggctaaacta tggaaaataa
      121 atatacacat ggtgtcctct tttaccatga acacagtgga ttaaaaaata ttaatcaagg
      181 tattggggaa gttacaacag cattaagttc aatttgtaag catctttcta ttcaattgag
      241 tgaaaacgaa ggtgacatta ttaaatattg ccaagaaatc aaaactaaaa actatgcaaa
      301 agatgtagat atattattta ttcttggcgg tgatggcacg gttaacgaac ttataaacgg
      361 tgttatgaca catgaccttc aactccctat tggcatttta ccaggcggta cttttaatga
      421 ttttacaaaa acgctaaata tagcacctaa tcataaacaa gctagtgaac aaatgatttc
      481 tgcacaagtt ggtacatacg atgtaattaa aataaataat caatatgcac tcaactttgt
      541 tggcttaggt ctcattgttc aaaacgctga aaacgtacaa gatggttcaa aagatatatt
      601 tggtaaattg agttatattg gatcaactgt caaaacactg ctaaatccaa cacaatttaa
      661 ttatcaatta tcaatagatg ataagacata ttctggcgaa acgaccatga tactaacagc
      721 aaatggtcca ttcattggtg gtagtcgtat accattgact gatttgtctc ctcaagatgg
      781 tgaactgaac actttcattt ttaatgaaca aagtttcagt attttaaatg atatttttaa
      841 aaagcgtgat agcatgaatt ggaatgaaat tactcaaggt attgagcata tacctggtaa
      901 aaagatttca cttacaacag atccagcaat gaaagttgat attgatggcg aaatttcatt
      961 agaaacacct attgatattg aggttatacc aaatgccatt cagttactta ctgtcaatga
     1021 tttgtaagca tccggaatga tcttaacccg atattatatt aggtttggag tgtaatcata
     1081 agattcaacg caccttatat tcgtttttat tagctttaag ctattaccct gtttcaaaaa
     1141 cagacatata attttggcgc atgccactaa aatgattgat gttgtatttt aaatcatagt
     1201 ggtttatgcg tctttttcaa attctataaa aaatcggatg acgtgtaatc tgccatagat
     1261 taacacattc atccgattta taataataag atagactaac atttattgag agcgggacgg
     1321 aaatgataaa gaacgactaa tgattgatta tgtagcgatt ctttatcatt agtcacagct
     1381 aatgtgtact taaaaatatg aatgcatgag ttacactcat gcataagaaa tactaatttc
     1441 taaagaaaaa gtatttcttt atgttggggc cccgccaact tgcattgtct gtagaaattg
     1501 ggaatccaat ttctctatgt tggggccccg cagtcaacta ctgccaatat agtgttgtag
     1561 tgtctaagac ataaaagttc atctcagtca caattacttt atagcctatc catcatcttt
     1621 tctacatgaa atttttcaag tgatatattt tatttctcaa tattatctaa aaaatactgt
     1681 tctacatctt tccagctatt tacgcgttca aatcgatcat cattaatatt atgcactgct
     1741 gtaaacataa tcggtgtacc agtaaaaatt tcaagctgtc taggattgtc atctattaaa
     1801 taatcagctt taacgatgtt ttttctacca caaaaaacaa aatgctgagg atctaaaaat
     1861 ggaaagaact ctagtaacca ttcatattta tcactaaatg atgttggtac atccattgct
     1921 gctgtagcaa tatatacatc ataatgttca gttaattttt tcacaacttc ttgtgcatac
     1981 ggcatcactt taagatgtct gaagaagcct ggttctctca atacttctgt aattaatcca
     2041 tcatgttcag gaataacatg tttaagtttt tgaccattca aagcttccat tttaatacct
     2101 aaatccgctc taaaattgac agcatcaatg atttctccta atgtatctgc caatacttca
     2161 tccatatcaa tcgcgattga tttacgggtc attttaatct tctcctcaaa atatcagtat
     2221 tgattcagta tagcaaaaaa gcaattgata caaaacaata tcatctattg ttgcttttta
     2281 cactgaatac tctcacctca tcattcacac tgcattgagc ttgagacata aatcaatgtt
     2341 caatgctcta caaagttata ttggcagtag ttgactgaac gaaaatgcgc ttgtaacaag
     2401 cttttttcaa ttctagtcaa ccttgccggc ggggccccaa caaagagaaa ttggattccc
     2461 aatttctaca gacaatgcaa gttggggtgg gacgacgaaa taaattttgc gaaaatatca
     2521 tttctgtccc actccccact tactattcgt atttaaagtt tgataaaact tctaacattt
     2581 tatcattttg ttctttaaaa ccaattgtaa ttctaacacc agttggaaat ggtcgcgtaa
     2641 tacaccctac atttaaaagt gcttcataaa gttcatttac ccgctttgtt tttacaaata
     2701 taaaattcgt ttgacttggc aagaaatact cactttgagg taattgataa aatctttcgc
     2761 gttcaacact atttttatgt gttacctctt ttaaatattg ttgatcttca agtgctgcaa
     2821 ctgctgcgta ttcagatata cgtgtcacat taaatggtgg tctaatgatg ttccattttt
     2881 caatcgcatg ttcacttgct accacatatc ctacacgtaa acccgctaat ccatacgcct
     2941 ttgaaaatgt acgtaataag aaagcattgt catatttttg ttgcaaagca agtgtatccg
     3001 ggtagtcctc tgctgtcaca aattcaaaat aagcttcatc tataattact ggtacatgtg
     3061 gaggtacttg agataaaaac gaatctaagc tctcatgatt aaaatatgta cctgtaggat
     3121 tatttggatt acataaccat accaatgaca tatcttcatt aacttctttt aaaatacctt
     3181 ctaaatcgaa gccaccatct tttaaaggta cttgtatcac attagctgat tcaacaatcg
     3241 cattgtgata atattgaccg aatgtcgctt cacttgtaac aatagtatcc cctggcgtta
     3301 atacagctct agaaatcatt aatataactt catctaatcc cgcaccaaat aaaatgcgtg
     3361 attgatctac atttaaatgt ttactaatcg ccgcttttaa tgtcggtgat cctgtttcag
     3421 gataataata taactcatct aagtgtgctg atatcgcttc tttaacttta ggcgatggtc
     3481 catacaaatt ttcatttgat gcaagtttat ataaatctcc ttcaatgcca tacttttctt
     3541 tcaatgccct tggagataaa ccaggctgat atgctgatag ttgattaagt tgttctttca
     3601 taataaaaac aacctcctta gaattaactg aaatttcaaa aaacttatat tcattatatc
     3661 gctctatgtt tctaaaatgc aatgctacta ttttcacatc ataatagctt acattgtgtt
     3721 taacatgtca tattttttaa tcattattgc atgtttaaat tgaacataat tatagattta
     3781 aatataacaa cgccacataa gatataattt ggtgttacgc atcttatgtg gcgttggtcg
     3841 ttatttgatt aacccttttg cttttaaata atctttcgca actgtatatg ggtcttcatt
     3901 tttaactgtt actttatagt tcatcatttg catatcttca tcagatattt tgttttctag
     3961 tttgtttaac ggtttcttaa tttctggatg tttctttaaa aagctttctt taaataatgg
     4021 tgctccttga tatggtggaa atacgtgctt atcatctttt aacacaacca tatcatattg
     4081 ttttaattca gcgtcagttg aatatgcatc tattaaatta atattacctt tattgatcgc
     4141 ttgatatctc aacttaggtt ccattgtacg tatgttatct aaatttaaat tataagcctt
     4201 ttgaacagct ttgtaaccat ctggacgatc attaaattcc aatgtaaatc ctggtttaag
     4261 ttgatcttta accttattta aatcaccaat tgtacgtata ttatgttgtt tagcaaaatc
     4321 acgttttaca gctaaagcat acgtattgtt atacttcatt ggttttaaca tagtcatttg
     4381 atatttcttt tcaagacttt gcttagcttg ttcataaact tttttctctt cttttgactt
     4441 caatggttct tttgttaatt cacctaaaac tgttccagta aattctaaat acccatctat
     4501 atcgtcagat tttaaagcat taaataaaaa tgctgttttg cccataccat ctttcacttc
     4561 tacagtattt ttggtctctt cttctattaa aattttatac atatttgtaa taatcgatgg
     4621 ctcggagcca agctttccag ctaacgtaat tttatcacct ttttgtgcaa acataggaat
     4681 agcgatagcc agtataataa tcatcactat aacacctaac gtcatcaata actttttata
     4741 agataattta gccataaatc ttaaaattaa atcaaatata attgccaata aggctgctgg
     4801 aattgcacct aataatatca acgatgcatt gttacggtct atacctaata aaattaaatc
     4861 tcctagtccg cctgcaccaa ttaatgctgc tagtgttgct gtacctataa ttaataccat
     4921 agccgttctt acaccagcca ttataacagg cattgctatc ggaagttcga ctttagttaa
     4981 acgtctaaat ggtttcatac ctataccttt agccgcttca atgagtgatg gatcaacttc
     5041 tttaattcca gtatacgtat tccttaaaat tggtaacaac gcatacacta caagtgcaat
     5101 aattgctggc acacgaccga taccaaataa aggaatcatt aaacctaata atgccaacga
     5161 tggtatggtt tgaagaattg ccgcaatatt cattacgatt tcagatatcg ttttagtctt
     5221 cgttaataaa atacctaatg gtaccgcaat agcagttgca atcaataatg cgataaatga
     5281 tatttgaata tgttctatca ttgtcgaaaa gagttgcccc ttacgttcac tcaatatgtc
     5341 gaaaaagtta gtcatgttga gctacctcct ttttctggga caaatatttg aagatatctt
     5401 tcctatcaat aacatattga cctacgctat cttcttgcat gacaatgaca cgctcgctct
     5461 ctgataaaag ttgatacaat acttcaattg gttgattgtc ataaacaatt ggataagcgc
     5521 tcatagatgt aacctcatcg attggtttca taatatccaa gtcacggata attgcgttct
     5581 cttcaacaca tggcgcatca tcttctaaat gactacccat aaattgttta acaaattcac
     5641 tttgaggatt atttttaaat ccttctggtg tgtcaatttg ttcaatatgc ccttcattca
     5701 aaagacaaat cttatcacca agtttcatcg cctcttgaat atcatgtgta acaaatatga
     5761 ttgtcttctt aattttagtt tgtaattcaa ttaaatcatc ttgaagtttt tctcggctga
     5821 ttgggtctaa tgcactaaac ggttcatcca ttaaaataac tggtggatca gctgctaacg
     5881 cacgtataac tcctacacgt tgtcgttgcc cccctgacaa ttcatcaggt tttctgtttt
     5941 tatatttttc aggttctaat ccaaccattt caagtaattc atctactctt ttatctatat
     6001 ctttttcttt ccactttttc atttgtggca cttgtgcaat attttctttg attgtcatat
     6061 gtgggaataa tgcaatctgc tgcaatacgt atccaatatc ccaacgcatt tcgtatactg
     6121 gataatcact tattggttta tctttaaaat aaatataacc ttcacttaag tgaatgagtc
     6181 gattaatcat ttttaatgtc gtagtttttc cacaacctga aggtccaatt agcacaaaaa
     6241 attcaccctc attaatattg aaactaatgt tatcgacagc aacatgtttg ccataacgct
     6301 tagttacatt tttaaactta atcactttgc cacctctttt tttctcatag cataaaaccg
     6361 agattatatg tatgtattcc ctatttaacc acgtttatta caattttcaa atttaaatga
     6421 tttatccttg aactttttta ataaaataat gaataatagg taatctccag ttaagaaata
     6481 gtgttatttt accttgaatt caaaaaaaca cccagtaaaa caaggaatgc ttactaggtg
     6541 tcttcactat actttggctt tataattttg aatcgtttct aaaaatgctg gacaataatg
     6601 ttttaatttg taactaccta cgccatcaat attaatcata tcttgtttcg aagcaggctt
     6661 acgtttagca aattcctcca acgtgtaatc agaaaatata cttacaggtg ctatcgttaa
     6721 tttgtcactt aactttttac gaacttctac caactgactg aataatactc ggtcaacccc
     6781 ttcaaccgta tttataaata ctttttcagt cgctttttgc ttaaatggtg ttgtgaatac
     6841 ttctacttca ttactgagta attttttaat tgaagtatca cacattaata tttcgtcatt
     6901 ttcatttaag aaccctttga atcttaattc atctattaag tgacttaatt ctgatgttgt
     6961 gtaacctttc attaaaccat gggttgaaat ttggtcataa cctttatact taatataatc
     7021 tgttgactct cctcttaaca cttgaatgat aacactataa ctctcttgtt gtttcatacg
     7081 agcgatgcaa ctaataatca tcttagcttc ttgtgtcata ttatatgatt tatcttgttg
     7141 aacacaatta ctacattgtt cacattcttc taatttttca ttcggttcaa aataatggac
     7201 aattgttgct tctagacatt tttttgtttt tgtatattga atcattttag ttaacttttc
     7261 gcccatttta tctttatagt catcatcagc ttgagagact gttataaaat actcgtgtaa
     7321 attgatatcg cgttcgctaa ataacaaaat acattcactt ttcaacccgt cacgacctgc
     7381 acgacccgct tcttgataat aagattctaa atctccaggc atattataat gaataacaaa
     7441 gcgtacattg gatttatcaa tacccatacc aaaagcattt gtagcaacga ctactttaac
     7501 acgatcaaat aagaaatcat tctgcgcttc ttctctttct ttattgctca aacctgcatg
     7561 atatataaca ctttcaattt tctgactttc taaggcttct tgaagctctt caacttgctt
     7621 acgtgtagaa caataaataa tacctgcatc ttcatcgtgt gttttaatat aatccaatat
     7681 aaatttttga cgttgataag taggatttac tttaaaaatt aagtttctac gcttagtact
     7741 cgttttaatt tgatcagttt gagcgatatt taacttttct ctaatatctt gctgtacttc
     7801 aaccgtggca gttgctgtca acgctattat tgtaaaatct tgaggtaacg taaatacttt
     7861 tgaaataaca ttttggtaac tcggcctgaa atcatgaccc catttagaaa tacaatgcgc
     7921 ttcatcaaac gcgactaagt gaatctttat acgctgaagc atatttaaaa aatatcggtt
     7981 ttcaaatcgt tctggtgcaa catacaaaaa ttgaatttct ccatttgata atgctttttc
     8041 aatacgttgt tgctcttttt gagtcaaact actatttaaa aaagcagctt gaattcccat
     8101 cgcttttaat tgatccactt gatctttcat taatgatatt agtggactta ttacaattgt
     8161 tgtaccacct aacaataaac ctggtacttg atagcatata gacttacctc caccagttgg
     8221 taagacacca agcacattac gatggtctaa tactttgcta ataatttctt cttgtcctgg
     8281 tcgaaacgtt tcatacccaa agtaatgcga taatgtttgt tgcatcataa attgacccct
     8341 tattgttgtt ctttaatttc ttctaactca ctccatcttg tgatgtctaa atcatattga
     8401 atttcaagtt gttctttttc ttcgtttaat tctttaattt tcccataatc tgcacttgcc
     8461 tcaatcatga gcacatcaat ttcttccatt cttacttccg cttgttctat gcgtttcatc
     8521 aattgttcat attctaattt ttctttatat gataaaccat ttttcttacg tacagttgta
     8581 gaagatttag attgttgctt caatgtggat ttatttttat ctaatgattt tttataactt
     8641 tcataatctt caaaagttcc gataatcttt tccatctgac catcatgaat aaaccaatat
     8701 gactgtgcaa ctttatttaa gaagtagcga tcatggctta cggtaatcac tgtaccaccg
     8761 aaagtatgaa tataatcttc aagtattgtt aaagtctcag tatctaaatc atttgtcggt
     8821 tcatctaaca acagaacatt tggctggtgt acgagtagac gtaataaata caaacgcttt
     8881 tgctctccac cagataattt ataaactttt ttaccatgag ttgcacttgg aaataaaaat
     8941 cgttcaagaa gttgtgtaat agataccgag gttccatctt tttcttttgc gatctcactt
     9001 tcttctctta aataatcaat catacgaata tctctatcca gggtctcatc tgtttgctta
     9061 aaataagcta ctttaaccgt ctgcccagtc ttcaatttac cttcgaattg ttggtcttct
     9121 ccactcaaaa tatttaagag tgttgtttta ccagctccat ttggcccaac aacaccaata
     9181 cgctcacctt tttgaataat ttccgtcaga tgttcaaata atactttatc attaatagcc
     9241 tttgataagt cttctaattc gaacacttgc ttacctaatc ttgaataagc aagattcaat
     9301 tcacctttat cgtctttata ttgctggtta acttcatttt ctaggtcatt aaatcgatta
     9361 attctagctt gttgctttgt agtacgagcc ttagctccag ccctcatcca agcaagttct
     9421 tccttatata aagctcgttg cttttgttgt tgcttttgaa gtgttacttc tctttcagcg
     9481 cgcatttcaa tataagattc atagttacca ggatatgacg ctaacttacc tctgtttagt
     9541 tcaataattc tagtggaaac ttcatttaaa aaatatcgat catgggttac gaataaaaca
     9601 gtatgaggat attgcttcac ataattgatc aaccagctga ttgattcgaa gtctaaatgg
     9661 ttcgtaggtt catctaataa caataaatct ggttgttcta ttaatgtttt agcaagtaca
     9721 acacgttttt gttgtccgcc ggataattct ttaatgtatt tagtagtatc atgtatacct
     9781 agttttgaga ggattgtttt aatttcagcg ttatagtccc aagcatcatg ttgatccata
     9841 gcatcttgcg catccatcat tcgcttgaac aatttatcac tttggtcatc cgcataagct
     9901 tgtactgcct gctcatattg cttgatgatg cgtaaagttg ttgtatcaga acttaatact
     9961 gcatcaaaaa ctgtcatatc ttcattaagg tcctgtttct gagacgaata tcgaattcga
    10021 tattgatttg gatgcataac attggctgta aaatcatcat caataccacc aattactttt
    10081 aacaacgtac ttttccctgt accatttatg cctactaaac ctattttttc accttctgaa
    10141 attgataaat ctaggttatc gaatatagtc ttatcggcat aagatttatt taaatgttca
    10201 attttatatg cttccatacc catcttcctt cttctgttga aatctctacg tacttaaatc
    10261 agttctatac attatacacc tttttgaaat caaaagaaat cgataccgtt aagtgtcata
    10321 atttaatata ttgttgcttc aactataaat aaaacttgtt aaggctttga tgactatgct
    10381 aatgatcttt ataatatacc tttttatcaa cacatataat gtaaaacata atataaaaga
    10441 acataataaa aacccggatt gatattttat caatccgagt tcgtgtttag atattatttt
    10501 ttagagtttg ctttaggtcc tgtttcatat ttatacttcg aaggatttac ctttttgaag
    10561 tctggatttt tgtagaatct aaacaaatca ccattaagca cgttgtcact catttcgaga
    10621 tccttttcaa cttgcttttt attcttttcg aaatcagctg gttgagtagt tatgagttca
    10681 ttatttttat tagaataaat cttaccatta acatatttat aatcttttgt tataaagtca
    10741 ccatttctga atggaactac ttgattatga cctttagaga ataaatcagt accgaacatt
    10801 aaatagttct tcgtatctat accagccaaa tgtaaaattg ttggcattac atcgacttga
    10861 ccagcatatt cattattgat accaccagat ttaccaggga ttttaatcca gaaaccagtt
    10921 ctgtttaaat ctgtaaattt agccggtgtg attttttcac ctaatagttt ttccatggca
    10981 ttgttatggt tttcagagat accatagtgg tcaccataaa tcataatcac tgaattgtca
    11041 tataatcctt ttttcttcaa gtcattaata tattcttcta atgcttcgtc taaataacgt
    11101 gctgtttgaa tataaccatc aactgttgca tcacctgtgt ttgatttctc aatagttgca
    11161 tccttttcat ctaatgtgaa tggatagtgg ttagtcaatg taattaaatg agaatagaat
    11221 ggtgatttca tcttagcttg ataattagca gaatctttaa agaaaatttt gtctttcaag
    11281 cctaagttta caacgttttt atctgacatg tcatagtatg ttgcatcata gaatttatcg
    11341 ataccaaagt gtttatatac ttggtctctg ttccagaatg ttttatagtc accgtgcatg
    11401 acatcagatt tgtagccttg cttttgatct aaaattgctg gtaatgactg atacgtatta
    11461 tctcctttta atgaaaaggc agaaccttgc ggtaaaccgt ataaactgtt atccattgta
    11521 aattcagagt cagatgtttt accttgacct gtttgatgga aaaagttagg gaagtatgtg
    11581 aattgctctt tcccacttga taatttgttt aaaaacggtg ttacttcttt accattaacc
    11641 tttttattaa ttaagaaggt ttggaaactt tctaaatgaa tcttaataat atttttcttc
    11701 tttgccaccc cataatattc tgggttaggc tctgtttgac gttgtttcgt ataatttaat
    11761 acttttgtta agtcatcttc agatgctagc gctttttgtt gattattttc gatagtttta
    11821 acaccatcgt atactgtaaa gttataaggt cctaaatatt tcactaaata tttatggtca
    11881 aatgtacgtg ttaataattc tggtctgtca gtttcagcaa aagctaagtt taagaagaat
    11941 aatgctactg aagccgccat tacgacagga acaaatttct tactaaatgc ttttgtgtct
    12001 aaccattttg ttttaaatat taaaatgaat aagtaaacta acgtatcaat gaaataaaca
    12061 aagtcatacc atttgaatga tgcactaacc gcaccaccca tagattctac gttacctact
    12121 tggtttaaag tactaaacgt taaaaaatca gagaagaatc taaagtacac aacattggca
    12181 tataatagga acgtcaataa gaagccgcct atgaacatga accaaaatgc ttttttgcct
    12241 ttaaagaata ggaacacact taaaaccagt gctactaaac tataaggatt catcaataat
    12301 attaagtttt gtactaaacc tttaacacct aaagaaaaat caacataata agaaaaatac
    12361 gtcttcaagg taatcgttat tacggttaat aagaagaacg caaaaagact aattttcttt
    12421 ttttgtgaac tcatgattct ttcccccgtt atttagataa taaatcttgc gaaagtactt
    12481 gaataaatta gatattaaca ttatgtagtt aattaataag tgcgtttaaa agttaacatt
    12541 gtcataatta tagtaattac tttcagattc aagtt
//
LOCUS       DAXWFK010000071        12541 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.071, whole genome shotgun sequence.
ACCESSION   DAXWFK010000071 DAXWFK010000000
VERSION     DAXWFK010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12541
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.071"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(177..1079)
                     /gene="cvfB"
                     /locus_tag="ACGEXS_001497"
     CDS             complement(177..1079)
                     /gene="cvfB"
                     /locus_tag="ACGEXS_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HFF8012510.1"
                     /translation="MALDKDIVGSIEFLEVVGLQGSTYLLKGPNGENVKLNQSEMNDD
                     DELEVGEEYSFFIYPNRSGELFATQNMPDITKDKYDFAKVLKTDRDGARIDVGLPREV
                     LVPWEDLPKVKSLWPQPGDYLLVTLRIDRENHMYGRLASESVVENMFTPVHDDNLKNE
                     VIEAKPYRVLRIGSFLLSESGYKIFVHESERKAEPRLGESVQVRIIGHNDKGELNGSF
                     LPLAHERLDDDGQVIFDLLVEYDGELPFWDKSSPEAIKEVFNMSKGSFKRAIGHLYKQ
                     KIINIETGKIALTKKGWSRMDSKE"
     gene            1226..2827
                     /locus_tag="ACGEXS_001498"
     CDS             1226..2827
                     /locus_tag="ACGEXS_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFF8012511.1"
                     /translation="MLQVTDVSLRFGDRKLFEDVNIKFTEGNCYGLIGANGAGKSTFL
                     KILSGELDSQTGHVSLGKNERLAVLKQDHYAYEDERVLDVVIKGHERLYEVMKEKDEI
                     YMKPDFSDEDGIRAAELEGEFAEMNGWNAEADAANLLSGLGIDPTLHDKKMAELENNQ
                     KIKVLLAQSLFGEPDVLLLDEPTNGLDIPAISWLEDFLINFDNTVIVVSHDRHFLNNV
                     CTHIADLDFGKIKVYVGNYDFWYQSSQLAQKMAQEQNKKKEEKMKELQDFIARFSANA
                     SKSKQATSRKKQLEKIELDDIQPSSRRYPFVKFTPEREIGNDLLIVQNLSKTIDGEKV
                     LDNISFTMNPNDKAILIGDSEIAKTTLLKILAGEMEPDEGSYKWGVTTSLSYFPKDNS
                     EFFEGVNMNLVDWLRQYAPEDEQTETFLRGFLGRMLFSGEEVKKKASVLSGGEKVRCM
                     LSKMMLSSANVLLLDEPTNHLDLESITAVNDGLKSFKGSIIFTSYDFEFINTIANRVI
                     DLNKQGGVSKEIPYEEYLQEIGVLK"
     regulatory      3429..3604
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            3686..4891
                     /locus_tag="ACGEXS_001499"
     CDS             3686..4891
                     /locus_tag="ACGEXS_001499"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFF8012512.1"
                     /translation="MVTRSVLKFGGSSVSDFTKIKRIAEMLKERVNQDEQLIVVVSAM
                     GNTTDQLMTNVSTLTKAPKQQELALLLTTGEQQTVSYLSMVLNDIGMNAKAMTGYQAG
                     IKTIGHHLKSKIAQINPQTFEQAFQENDILVVAGFQGINEHQELTTLGRGGSDTTAVA
                     LAVSNQIPCEIYTDVDGVYATDPRLLPKAKRLDIVSYEEMMEMSALGAGVLETRSVEL
                     AKNYNIPLYLGKTLSNVKGTWIMSNEEILEKKAVTGVALDKHMMHVTISYPLPDNQLL
                     TQLFTELEEGAVNVDMISQIVNLDGLQLSFTIKDSDFHQISMILETLKNQYEALAYKI
                     NEHYVKISLIGSGMRDMSGVASKAFLTLIENNIPFYQTTTSEISISYVIDDFNGQQAV
                     EKLYDAFNI"
     gene            4955..5944
                     /locus_tag="ACGEXS_001500"
     CDS             4955..5944
                     /locus_tag="ACGEXS_001500"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFF8012513.1"
                     /translation="MTKLAVVGATGLVGTKMLETLNRKNIPFDELVLFSSARSAGQEV
                     EFQGKTYTVQELTDARASEHFDYVLMSAGGGTSEHFAPLFEKAGAIVIDNSSQWRMAE
                     DIDLIVPEVNEPTFTRGIIANPNCSTIQSVVPLKVLQDAYGLKRVAYTTYQAVSGSGM
                     KGKKDLAEGVNGKAPEAYPHPIYNNVLPHIDVFLENGYTKEEQKMIDETRKILNAPDL
                     KVTATCARVPVQDSHSVEIDVTLDKETTAEDIKALFDQDDRVVLVDNPENNEYPMAIN
                     STNKDEVFVGRIRRDDSLENTFHVWCTSDNLLKGAALNAVQVLEQVMRLKGAN"
     gene            5946..6833
                     /gene="dapA"
                     /locus_tag="ACGEXS_001501"
     CDS             5946..6833
                     /gene="dapA"
                     /locus_tag="ACGEXS_001501"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFF8012514.1"
                     /translation="MTHLFEGVGVALTTPFTNNKVNIEALKTHVNFLLENNAQAIIVN
                     GTTAESPTLTTDEKERILKTVIDLVDKRVPVIAGTGTNDTEKSIQASIQAKALGADAI
                     MLITPYYNKTNQRGLVKHFEAIADAVKLPVVLYNVPSRTNMTIEPETVEILSQHPYIV
                     ALKDATNDFEYLEEVKKRIDTNSFALYSGNDDNVVEYYQRGGQGVISVIANVIPKEFQ
                     ALYDAQQSGLDIQDQFKPIGTLLSALSVDINPIPIKALTSYLGFGNYELRLPLVSLED
                     TDTKVLRETYDTFKAGENE"
     gene            6830..7552
                     /gene="dapB"
                     /locus_tag="ACGEXS_001502"
     CDS             6830..7552
                     /gene="dapB"
                     /locus_tag="ACGEXS_001502"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFF8012515.1"
                     /translation="MKILLIGYGAMNQRVARLAEEKGHEIVGVIENTPKATTPYQQYQ
                     HIADVKGADVAIDFSNPNLLFPLLDEDFHLPLVVATTGEKEKLLNKLDELSQNMPVFF
                     SANMSYGVHALTKILAAAVPLLDDFDIELTEAHHNKKVDAPSGTLEKLYDVIVSLKEN
                     VTPVYDRHELNEKRQPQDIGIHSIRGGTIVGEHEVLFAGTDETIQITHRAQSKDIFAN
                     GAIQAAERLVNKPNGFYTFDNL"
     gene            7579..8298
                     /gene="dapD"
                     /locus_tag="ACGEXS_001503"
     CDS             7579..8298
                     /gene="dapD"
                     /locus_tag="ACGEXS_001503"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFF8012516.1"
                     /translation="MVQHLTAEEIIQYISDAKKSTPIKVYLNGNFEGITYPESFKVFG
                     SEQSKVIFCEADDWKPFYEAYGSQFEDIEIEMDRRNSAIPLKDLTNTNARIEPGAFIR
                     EQAIIEDGAVVMMGATINIGAVVGEGTMIDMNATLGGRATTGKNVHVGAGAVLAGVIE
                     PPSASPVIIEDDVLIGANAVILEGVRVGKGAIVAAGAIVTQDVPAGAVVAGTPAKVIK
                     QASEVQDTKKEIVAALRKLND"
     gene            8441..9592
                     /locus_tag="ACGEXS_001504"
     CDS             8441..9592
                     /locus_tag="ACGEXS_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HFF8012517.1"
                     /translation="MNELEFVTKHRRHLHQHPELSLHEFETTAYIKAFLDSLNIKYDC
                     PLETGVIAYLEGNGSHTIAYRADIDALPILEENNVPYRSQSDHVMHACGHDGHTTALM
                     LFVQRCKDMQDAGQLPQNVVFIFQPAEETGGGANRLIKAGAFDKYPIEAVFGIHVNPF
                     ADEGIAVIRDEEITASATEYRFFSTGLSSHVADKEQGHSCGEALQHVLTQISQIQQFH
                     LNGLKRNIVHIGHFKAGEAINTVPSNGYLEGTIRTYDIDDLTIVKNQMHKIAESVKLL
                     FNVDCEVKFAEGYPPTINSPKLRTQIEDALIKADLNVYDKPTPFLFGEDFSFYGQQLA
                     PAYFVFIGTRNEDKGFVTGLHTSHLNFDEKVLINVVNFYENLLNNYKEV"
     gene            9597..10682
                     /locus_tag="ACGEXS_001505"
     CDS             9597..10682
                     /locus_tag="ACGEXS_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFF8012518.1"
                     /translation="MTATWSVNKKIFLQNAITVKNNQPLMAVVKNNAYHYDLEFAVTQ
                     FIHAGIDTFSTTSLREAIQIRQLAPDATIFLMNAVYEFDLVREHQIHMTLPSLTYYYN
                     HKNDLAGIHVHLEFENLLHRSGFKDLNEIKEVLKDHHHNQNAKMIISGLWTHFGYADE
                     FDVSDYNVERSQWMEIVEALLSEGYQFDLIHAQNSASFYREGQILLPHHTHARVGIAL
                     YGSRPYSSLNQHDIVQSLTLKAHVIQVREVQAGDYCGYSFAFEVTKNNTKLAVVDIGY
                     GDGILRTRAKHEALINGKRYPIRALMMSHMFVEVDGNVHAQDEVILYNNDIRIDEYTF
                     KGVGANSEQLSAMNHDSLKKEYISNDC"
     gene            10672..11937
                     /gene="lysA"
                     /locus_tag="ACGEXS_001506"
     CDS             10672..11937
                     /gene="lysA"
                     /locus_tag="ACGEXS_001506"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFF8012519.1"
                     /translation="MTVKYNQNGELTMDGISLKTIAQSFGTPTIVYDELQIREQMRRY
                     HRAFKDSGLKYNISYASKAFTCIQMVKLVAEEDLQLDVVSEGELYTALEAGFEPSRIH
                     FHGNNKTKHEIRYALENNIGYFVIDSLEEIELIDRYANDTVQVVLRVNPGVEAHTHEF
                     IQTGQEDSKFGLSIQYGLAKKAIDKVQQSKHLKLKGVHCHIGSQIEGTEAFIETAKIV
                     LRWLKEQGIQVELLNLGGGFGIKYVEGDESFPIESGIKDITDAIKSEIKVLGIDAPEI
                     GIEPGRSIVGEAGVTLYEVGTIKEIPEINKYVSIDGGMSDHIRTALYDAKYQALLVNR
                     NEEADDSVTIAGKLCESGDIIIKDAKLPSSVKRGDYLAILSTGAYHYSMASNYNQMQK
                     PSVFFLKDGKAREVIKRQSLRQLIINDTK"
     gene            complement(12177..>12541)
                     /gene="msaC"
                     /locus_tag="ACGEXS_001507"
                     /gene_synonym="msa"
     CDS             complement(12177..>12541)
                     /gene="msaC"
                     /locus_tag="ACGEXS_001507"
                     /gene_synonym="msa"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sarA expression modulator MsaC"
                     /protein_id="HFF8012520.1"
                     /translation="VFGVLSAIGLNINILAAMMIVLVIPIMISGILFFKTNIDKTYIF
                     FNIIFIDFYYYIYNVHLMTLPKFNNYIKAEMMELEDIDVLITSKDFGFDEILFYTLYL
                     LLILIVLYYLKKQVKHKI"
BASE COUNT         4391 a         1969 c         2266 g         3915 t
ORIGIN      
        1 atcaaataat taatagaaat cagctttttt acattgccta agaacttaat gtcccagcct
       61 attcgtttga attatttaat tttttacttt gaaaggaatg tttaaaaaac tacttcatct
      121 gtccgattta atattgaata aaaaaaacac gcatcctacg acacgtgtaa aaatgattat
      181 tcttttgagt ccattcgact ccaacctttt ttagttaaag cgattttacc tgtttctata
      241 ttaataatct tctgtttata taagtgaccg attgcacgtt tgaatgaacc tttactcata
      301 ttgaatactt ctttaatcgc ttcagggctt gatttgtccc agaatggtaa ttcaccatca
      361 tattcaacta gtaaatcaaa gatgacttgg ccgtcatcgt ctaaacgttc atgtgcaagt
      421 ggtaaaaatg aaccatttaa ctcaccttta tcattatgcc cgataattct aacttgaaca
      481 gattcaccta atcttggttc agctttacgt tctgattcat gtacgaaaat tttgtaacct
      541 gattcgctta ataaaaagct accaattcgt aatacgcggt aaggtttggc ttcaatgact
      601 tcgtttttta aattatcgtc gtgtacaggt gtaaacatat tttctacaac agattcactc
      661 gctaaacgtc catacatatg attctcacgg tcaattcgta atgtaactag caaataatca
      721 ccaggttgtg gccatagtga tttcactttt ggtaaatctt cccatggtac taacacttca
      781 cggggtaatc caacatctat acgtgcccca tcgcgatccg ttttaagtac tttagcaaag
      841 tcatatttat ctttcgtaat atcaggcata ttttgagttg caaataattc acctgaacgg
      901 tttggataaa tgaagaaact atattcttca cctacttcta attcatcatc atcgttcatt
      961 tctgattggt ttaactttac gttttcaccg tttggtcctt ttaaaaggta agttgaacct
     1021 tgtaacccta ctacttcaag gaattctata gaacctacta tatctttgtc taatgccatg
     1081 ttattctcct tcgattcttg tattctttat tattataaca tgtgtgagtc attctatcat
     1141 tacaacttat atgaggtcga aactgtgacg ataatatgat ataattcttc cgttagaata
     1201 tcgaatgaaa aaaaggagaa aatgcatgtt acaagtaact gatgtgagtt tacgttttgg
     1261 agatcgtaaa ctatttgaag atgtaaatat taaatttaca gaaggtaatt gttatggatt
     1321 aattggtgcg aatggtgcag gtaaatcaac attcttaaaa atattatctg gtgaattaga
     1381 ttctcaaaca ggacatgttt cattagggaa aaatgaacgt ctagctgttt taaaacagga
     1441 ccactatgct tatgaagatg aacgcgtgct tgatgttgta attaaaggtc acgaacgtct
     1501 ttatgaggtt atgaaagaaa aagatgaaat ctatatgaag ccagatttca gtgatgaaga
     1561 tggtatccgt gctgctgaac ttgaaggtga atttgcagaa atgaatggtt ggaatgctga
     1621 agctgatgct gctaaccttt tatctggttt aggtatcgat ccaactttac acgataaaaa
     1681 aatggctgaa ttagaaaaca accaaaaaat taaagtatta ttagcgcaaa gtttattcgg
     1741 tgaaccagac gtactattac tggatgagcc tactaacggt ctcgatattc cagcaatcag
     1801 ttggttagaa gatttcttaa ttaactttga taatactgtt atcgtagtat cgcatgaccg
     1861 tcatttctta aataatgtat gtactcatat cgctgattta gacttcggta aaattaaagt
     1921 ttatgttggt aactatgatt tttggtatca atctagtcag ttagctcaaa agatggctca
     1981 agaacaaaac aagaaaaaag aagaaaaaat gaaagagtta caggatttta ttgctcgttt
     2041 ctcagctaac gcttctaaat ctaaacaagc aacaagtcgt aaaaaacaac tcgagaaaat
     2101 tgaattagat gatattcaac catcatcaag aagatatcct ttcgttaaat tcacacctga
     2161 gcgcgaaatc ggtaatgact tactaatcgt tcaaaatcta tctaaaacga ttgacggtga
     2221 aaaagtatta gataatattt cattcacaat gaatccaaat gataaagcaa ttttaattgg
     2281 ggatagtgaa attgcgaaaa ccacattgct taaaatatta gccggtgaaa tggaaccaga
     2341 cgaaggttca tataaatggg gtgtaacaac gtcattaagt tacttcccta aagataactc
     2401 agaattcttt gagggcgtta atatgaacct tgtagattgg ttaagacaat acgcaccaga
     2461 agatgagcaa actgaaacat ttttacgcgg cttcttaggc cgtatgctat ttagtggaga
     2521 agaagttaag aaaaaagcta gtgtactttc aggtggagaa aaagtacgtt gtatgctaag
     2581 taaaatgatg ttatcaagtg caaacgttct tttacttgat gaaccaacaa accacttaga
     2641 cttagaaagt attactgctg ttaatgatgg acttaaatca ttcaaaggtt ctattatctt
     2701 tacttcatat gactttgaat ttatcaatac aatcgcaaac cgtgttatcg atttaaataa
     2761 acaaggtggc gtttcaaaag aaattccata tgaagaatat ttacaagaaa tcggcgtttt
     2821 aaaataaaca taatatgtag aatttctttt cgaaaatctc tgtattgagg cccgaccaac
     2881 ttgcacatta ttgtaagttg gcttactgtc accttctata ttgggcttat tccgcaactt
     2941 gtattgtttg tagaaattag gaatccaatt tctctatgtg ttgcaccgac aaggattgaa
     3001 aaaagctagc tacaaagtca ttttcattca gtcaactact gtcaatataa cattgcaggg
     3061 cctagggcat tgattcatgt ccgagactat attcaattta atagacatta actgggcgtc
     3121 tcgatgctaa tatttatagc atcgaggtgt cctttttatt ttatttttta acacatcagc
     3181 atagtgaaca ccccttctct aaattacaaa tacagttatc aacgcattaa aagagtacat
     3241 ttatttaatg tgcatattga ttatgctata agtattttga attttatttt tcaaaaagtc
     3301 tcattatcac caccttttaa aatataagca acgctataac ttgaattttc aggttattcc
     3361 aaaaaatcta aattatcggt ttacattcat atttgtcata tgtataatga acacatacca
     3421 atttaataat attttgatga ggcgcatcaa tcatgagtaa agtttagatt actgtctgct
     3481 aacagctaaa tttgaaaggg tgcgatgccg aagcaattat aatagcagtt ataatttgtt
     3541 ggactttttg gttaagagct gagagtttgt cattatttaa aaataatgga gtgcatcact
     3601 tgtatataga ttaagagcaa gttcgcattc cgaacttgtt ctttttttat tattgtgtgc
     3661 ccttcctaac aattaggagg attatatggt aacaagaagt gtgttgaaat ttggcggatc
     3721 atccgtcagt gattttacaa aaataaaaag gatcgctgaa atgttaaagg agcgagtcaa
     3781 tcaagatgaa caattaattg tcgttgtaag tgctatgggt aacacaacag atcaattaat
     3841 gacgaatgta tcaaccttga ctaaagcacc aaaacaacaa gaactggcat tattattgac
     3901 aaccggagag caacaaactg tatcttattt atcaatggta ttaaatgata tcggtatgaa
     3961 tgccaaagca atgactggct atcaagcggg tattaaaacc attggccatc atttaaaaag
     4021 taaaattgct caaattaatc ctcaaacatt tgaacaagcc tttcaagaaa acgatatttt
     4081 agtagttgct ggatttcaag gcatcaatga acatcaggaa ttaacaactt taggcagagg
     4141 tggttctgat acgaccgctg tggcacttgc tgttagtaat caaatacctt gtgaaattta
     4201 taccgacgtt gatggtgtgt atgccactga cccaagactt ttaccaaagg ctaaacgact
     4261 agacatcgtc tcatatgaag aaatgatgga aatgagcgct ttaggtgctg gtgtacttga
     4321 aacaagaagt gttgaattag ctaaaaacta taatatccct ttatatttag gaaaaacttt
     4381 atcgaacgtg aaaggaacat ggattatgtc aaatgaagaa atattagaga aaaaagcagt
     4441 tactggtgtg gctttggata aacatatgat gcatgtaaca attagttatc ccctacctga
     4501 caatcagcta cttacccaac tatttacgga acttgaagaa ggtgctgtaa atgttgatat
     4561 gatttcacaa atcgtcaact tggatgggct acaactatcc ttcacgatta aagatagtga
     4621 ttttcatcaa atttctatga ttcttgaaac attaaagaat caatatgaag cattagctta
     4681 taaaatcaat gagcattatg tcaaaatttc attaattggc tcaggcatgc gtgatatgtc
     4741 aggtgtggca tcaaaagcat ttttgacatt aattgaaaat aatatacctt tctaccaaac
     4801 aacaacatct gaaataagta tttcatacgt cattgatgat tttaatgggc aacaagcggt
     4861 agaaaaacta tatgacgcat ttaacattta atggtaaaat gattgttaaa atattctaaa
     4921 aattggaaat tattataaaa tggagtgaca agttatgaca aagttagcag ttgtgggtgc
     4981 aacaggatta gtaggtacaa aaatgttgga gacattaaat cgtaaaaata ttcctttcga
     5041 tgaattagta ttattttcat cagcacgttc tgcagggcaa gaagttgaat ttcaaggaaa
     5101 aacatataca gttcaagaat taactgatgc tcgtgcaagt gaacatttcg attatgtatt
     5161 aatgagtgct ggtggcggta caagcgaaca ctttgcccca ctttttgaaa aagctggtgc
     5221 aatcgttata gacaattcaa gtcaatggcg tatggcagaa gatattgatt taatcgttcc
     5281 ggaagtcaat gaacctacat ttacaagagg tatcattgcc aatccaaact gctctacgat
     5341 tcaatctgtt gtacctctaa aagtattgca agatgcttat ggtttaaaac gagtggcata
     5401 tacaacatat caagctgtat caggttcagg gatgaaaggt aagaaagatt tagctgaagg
     5461 tgtaaatggt aaagcaccag aagcatatcc acatccaatt tataataatg tgttaccgca
     5521 tattgatgtg tttttagaaa acggatatac aaaagaagaa caaaaaatga ttgatgagac
     5581 gagaaaaatt ttaaatgcgc cagacttaaa agtaacagca acatgcgcac gtgtgcctgt
     5641 tcaagatagt catagtgttg aaattgatgt aacgcttgac aaagaaacaa cagcagaaga
     5701 tattaaagcg ttatttgatc aagatgaccg cgttgtttta gtagacaatc cagagaacaa
     5761 tgaatatcca atggcaatca attctactaa taaagatgaa gtgtttgttg gccgtatacg
     5821 tagagatgat tcattagaaa atactttcca tgtatggtgt acatcagaca atttattaaa
     5881 aggtgctgca ttaaatgctg tacaagtatt ggaacaagtt atgcgtttaa aaggagcgaa
     5941 ttaaaatgac acatttattt gagggtgttg gcgttgcact tacaacccct tttacaaata
     6001 acaaagttaa tattgaagct ttgaaaacac acgttaattt tttactagaa aataatgccc
     6061 aagcaatcat cgttaatgga actactgctg agagccctac tttaacaaca gatgaaaaag
     6121 aacgcattct aaaaacagtt attgatcttg tagataaacg tgttcctgtc atagcaggaa
     6181 ctggcactaa tgatactgaa aagtcaatcc aagcttcaat ccaagctaaa gccttagggg
     6241 ctgatgcaat tatgttaatt acgccctact acaacaaaac gaaccaacgt ggtttagtca
     6301 aacactttga agcgattgca gatgctgtga aattaccagt cgtgctgtac aatgttcctt
     6361 caagaacgaa catgacaatt gaaccagaaa ctgtagaaat attaagtcaa catccttata
     6421 tagttgcttt aaaagatgct acgaatgatt ttgagtattt agaagaagtg aaaaagcgca
     6481 ttgatacaaa ttcatttgca ttatatagtg gcaatgatga caacgtcgtc gaatactatc
     6541 aacgtggcgg tcaaggggtt atctctgtta ttgccaatgt cattcctaaa gaatttcaag
     6601 cgttatacga tgctcaacaa agtggattag atattcaaga tcaatttaaa ccaatcggca
     6661 cactgttatc agctttatca gttgatatta acccaattcc tattaaagct ctaacaagtt
     6721 atttaggatt tggaaattat gaattacgtc taccattggt tagcctagaa gatacagata
     6781 ctaaagtgct tcgtgaaaca tatgacacat ttaaagcggg tgaaaatgag tgaaaatatt
     6841 actaattggc tatggcgcaa tgaatcaacg cgttgctaga ttagcagaag aaaaaggaca
     6901 tgaaatcgtt ggggtcattg aaaatacacc gaaagcaaca acgccatatc aacaatatca
     6961 acatattgca gatgttaaag gtgccgatgt tgcaatagat ttttcaaatc caaatctgct
     7021 tttcccttta ttagatgaag attttcattt gccattagtt gtggcaacaa ctggcgagaa
     7081 agaaaaacta cttaataagt tagatgaatt gagtcaaaat atgcctgtgt ttttcagcgc
     7141 gaacatgagt tatggcgttc atgcattgac taaaatttta gcagctgctg ttcccctact
     7201 tgatgatttc gacatcgaat tgactgaggc acatcataat aaaaaagtag atgcaccaag
     7261 tggtacgtta gaaaaattgt atgatgtgat cgtatctttg aaagaaaatg taacacctgt
     7321 gtatgataga catgaattaa atgaaaaacg ccagccacaa gatattggta tacattctat
     7381 tcgtggaggt acgattgtcg gtgaacatga agttctattt gctggcactg atgaaacgat
     7441 tcaaatcacg catcgtgcac aatcaaaaga tatttttgcg aatggtgcaa tacaagcagc
     7501 agaacgctta gttaataaac caaacggctt ttatacgttt gacaacttat aaacatatta
     7561 aaggagatcg attattttat ggtacaacat ttaacagctg aagaaattat tcaatatata
     7621 agtgatgcta aaaagtctac accaataaaa gtatatttaa atggtaattt tgaaggcatc
     7681 acatatccag aaagttttaa agtatttggt tcagaacaat ctaaagtaat cttttgtgaa
     7741 gcggatgatt ggaaaccttt ttacgaagca tatggtagtc aattcgaaga tatagaaatt
     7801 gaaatggatc gtcgcaattc tgctattcca ttaaaagact taacaaatac gaatgcacga
     7861 attgaaccag gtgcgtttat tagagaacaa gccattattg aagatggtgc tgtcgttatg
     7921 atgggcgcaa caattaatat tggcgcagtc gttggcgaag gtacaatgat tgatatgaat
     7981 gctactctcg gtggtcgtgc tacaactggt aaaaatgtac atgtaggggc tggcgcagta
     8041 ttagcaggtg tgattgaacc ccctagtgct tcaccggtta taatcgagga tgatgtatta
     8101 atcggtgcaa atgcagttat tttagaaggt gtacgtgttg gtaaaggtgc tattgttgca
     8161 gctggcgcga ttgtgacaca agatgtacca gctggtgcag ttgttgctgg tacacctgca
     8221 aaagtgatta agcaagcttc tgaagtacaa gatactaaaa aagagattgt agcagcatta
     8281 agaaaactga atgactagtg acgtcaacgt ataataattt caaggttgag atacgattat
     8341 gtctcaacct tattgtttag cattgaataa acttatgatc acaaatttcg aaatacgtta
     8401 cactatttat atagtaattt aacacaaagg attgataatt atgaatgaat tagaatttgt
     8461 tacgaaacat cgccgtcatt tacatcaaca tcctgaatta agcttacatg aatttgaaac
     8521 aactgcttat attaaagcgt ttttagatag tttaaatatt aaatacgatt gcccattgga
     8581 aactggcgtc attgcatact tagaaggtaa tggctcacat acgatagcgt atagagctga
     8641 tattgatgcg ttacctattt tagaggaaaa taatgtgcct tatcgcagtc aatctgatca
     8701 tgtgatgcat gcttgtggac atgatggtca tacaactgca ttaatgcttt ttgtacaacg
     8761 ttgcaaagac atgcaagatg caggtcaatt accgcaaaat gtcgttttca ttttccaacc
     8821 tgcagaagaa actggtggcg gtgcaaatcg attaataaaa gccggtgcct ttgataagta
     8881 tccaattgaa gcggtatttg gtattcatgt taacccattt gctgatgaag gcattgcagt
     8941 gataagagat gaagaaatta cggccagcgc aacagagtat cgctttttct caacaggcct
     9001 gtcaagtcat gttgctgata aagaacaagg tcattcttgt ggtgaagcat tacaacatgt
     9061 attaactcaa atatcacaaa ttcaacaatt tcaccttaac ggtttgaaac gaaatattgt
     9121 tcatattggt cattttaaag ctggtgaagc gattaacact gtaccaagta atggctattt
     9181 agaaggtact attcgtacat atgatattga tgatttaaca atcgttaaaa atcaaatgca
     9241 caagatagca gaaagtgtca agcttctgtt taatgtagat tgtgaagtta aatttgcaga
     9301 aggttatccc cctacaatca atagtccgaa attacgtact caaatagagg acgccttaat
     9361 aaaagctgat ttaaatgtct atgacaaacc aacgccattc ttatttgggg aagattttag
     9421 tttttatggt caacaactag ctccagctta ctttgttttt ataggaacac gaaatgaaga
     9481 taaaggtttt gtaactggtt tgcacacatc acatttaaat tttgatgaaa aagtgttaat
     9541 aaacgtggtt aatttttacg aaaatttatt aaataattac aaagaggtgt aatacattga
     9601 cagcaacatg gtctgtaaat aagaaaatat ttttacaaaa tgcaatcaca gtcaaaaaca
     9661 atcagccatt aatggcagtt gttaaaaata atgcatatca ctatgaccta gaatttgctg
     9721 taactcagtt tatccatgca ggtatagata catttagcac aacatcacta cgagaagcaa
     9781 ttcaaattag acaacttgct ccagatgcaa caatcttttt aatgaatgca gtttacgagt
     9841 ttgatttagt ccgtgaacat caaatacaca tgactttgcc gtcgttgaca tattactata
     9901 accataaaaa tgatttagct ggtattcatg ttcacttaga atttgaaaat ttattacatc
     9961 ggtctggatt taaagattta aacgaaatta aagaagtatt gaaagatcac catcataatc
    10021 aaaatgcaaa aatgattatt agtggtttat ggacccattt tggatatgct gatgaattcg
    10081 atgtgtcaga ttataatgtt gaacgttcac aatggatgga aattgttgaa gcacttttat
    10141 ctgaaggtta tcagttcgac ctaatccatg ctcaaaatag tgcgagtttt tatcgggaag
    10201 gacaaatatt actaccccac catacacatg cgcgtgtagg tattgcgtta tacggttcaa
    10261 gaccatatag ttcactgaat caacatgata tagttcagtc attaacttta aaagcacatg
    10321 ttattcaagt gcgcgaagta caagctggtg attattgcgg ttatagcttt gcctttgaag
    10381 tgactaaaaa caatacaaaa ttagctgtag ttgatatcgg ttatggcgat ggaattttaa
    10441 gaactcgtgc taaacatgaa gcacttatca atggtaaacg ctacccgata cgtgcattaa
    10501 tgatgagcca tatgtttgtt gaagtagatg gcaatgtaca tgcacaagat gaagttattc
    10561 tttataataa tgatatccgc atcgatgaat atacctttaa aggtgttggt gcaaattctg
    10621 aacaattaag tgctatgaat catgattctt taaaaaagga gtacatttca aatgactgtt
    10681 aaatataatc aaaatggcga attaacaatg gatggtatta gtttaaaaac gattgcacaa
    10741 agctttggta cacctaccat tgtttatgat gaactacaaa ttagagaaca gatgcgccgt
    10801 taccatcgcg catttaaaga tagtggatta aaatacaata tttcatacgc ctcaaaggca
    10861 tttacttgca ttcaaatggt caaacttgta gctgaggaag atttacagtt agatgttgtt
    10921 tctgaaggtg aattatatac agctttagaa gcaggttttg aaccgagtcg catccatttc
    10981 catggtaaca ataaaacgaa acatgaaatt aggtatgctt tagaaaataa tatcggttat
    11041 tttgttatag attcattaga agaaattgaa ttaatagacc gctatgctaa tgatacggtt
    11101 caagttgtat tacgagttaa tccaggtgtt gaagcacata cacacgaatt tattcaaact
    11161 gggcaagaag atagtaagtt tggattatca attcaatatg gcttagctaa aaaagcaatt
    11221 gacaaagtcc aacaatctaa acacttaaaa ttaaaaggtg tacattgtca tattggttca
    11281 cagattgaag gtacagaagc atttattgaa actgctaaaa ttgttttacg ttggcttaaa
    11341 gagcaaggca ttcaagttga attattaaac cttggtggtg gctttggtat taaatatgtt
    11401 gaaggtgacg aaagtttccc tatcgaaagt ggtattaaag atattacaga cgcaataaaa
    11461 tccgaaatta aagttctagg tatagatgca ccagaaatag gtattgaacc gggacgatca
    11521 attgtaggtg aagctggcgt tactttatat gaagttggaa ccattaaaga aattccagag
    11581 attaataaat atgtttcaat cgatggcggt atgagtgatc atatcagaac tgcactttat
    11641 gacgcaaagt atcaagcatt gcttgttaat agaaatgaag aagcagatga cagtgtaact
    11701 atagctggaa aattatgtga gtctggtgat atcattatta aagacgctaa attaccttca
    11761 tcagtcaaac gtggagacta tcttgctata ttatcaactg gtgcatatca ttactctatg
    11821 gcatccaatt acaatcaaat gcaaaagcct tctgtgtttt tcttaaaaga tggcaaagca
    11881 cgtgaagtta taaagcgaca atcgttaaga caactcatta ttaatgatac aaaataaaaa
    11941 taaacaaagt aatccccgaa gcacagaaaa ttatactgtg actcggggaa tttttatgca
    12001 ttatagatga ctgtagaaaa catgcttgga catatactct aaaaaaggaa gaacagcaca
    12061 tttttagtta gaaagtattt atttcttttt gtcattaatc ctttatattt agttagccct
    12121 attcaactaa tttaaagcat catatacata ccacacttta aacaagcatc atgaaattag
    12181 attttatgct ttacttgctt tttcaaataa tataaaacga taagaattaa caacaaatat
    12241 aatgtataaa acagaatttc atcaaatcca aaatctttac ttgtaattaa tacatcaata
    12301 tcttctaatt ccatcatctc tgctttaata taattattaa actttggcaa agtcattaaa
    12361 tgtacattgt aaatatagta ataaaagtcg ataaatatga tgttaaaaaa tatgtatgtt
    12421 ttatcaatat tagttttgaa aaatagtatc ccagaaatca ttatcggaat cactaataca
    12481 atcatcattg ctgctaaaat attaatattt aatccaattg cagataatac accgaaaact
    12541 a
//
LOCUS       DAXWFK010000072        12500 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.072, whole genome shotgun sequence.
ACCESSION   DAXWFK010000072 DAXWFK010000000
VERSION     DAXWFK010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12500
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.072"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            3..902
                     /gene="opp3b"
                     /locus_tag="ACGEXS_001508"
     CDS             3..902
                     /gene="opp3b"
                     /locus_tag="ACGEXS_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012990893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFF8012521.1"
                     /translation="MYMLISLFIIITITFFLMKLMPGSPFNDAKLNAEQKEILNEKYG
                     LNDPVATQYLHYLKNVVTGDFGNSFQYHNQPVWDLIKPRLLPSFEMGLTAMFIGVILG
                     LILGVAAATKQNSWVDYTTTVISVIAVSVPSFVLAVLLQYVFAVKLRWFPVAGWEGFS
                     TAVLPSLALSAAVLATVARYIRAEMIEVLSSDYILLARAKGNSTMRVLFGHALRNALI
                     PIITIIVPMLASILTGTLTIENIFGVPGLGDQFVRSITTNDFSVIMAITLLFSTLFIV
                     SIFIVDILYGVIDPRIRVQGGKK"
     gene            902..1972
                     /gene="opp3C"
                     /locus_tag="ACGEXS_001509"
     CDS             902..1972
                     /gene="opp3C"
                     /locus_tag="ACGEXS_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFF8012522.1"
                     /translation="MAENKNNLSINDDHSNAAMTHTSDAIASSDFIIRELDLNQEPEM
                     QRESKNFWQDAWAQLKRNKLAVVGMIGLIIIVIFAFIGPVINKHDYAEQNVEHRNLPA
                     KIPVLDKVPFLPFDGKDADGKDAYKAANAKENYWFGTDQLGRDLWTRTWKGAQISLFI
                     GVVAAMLDIFIGVVYGAISGFFGGRVDTIMQRILEVIASIPNLIVVILFVLIFEPSIW
                     TIILAMSITGWLGMSRVVRGEFLKLKNQEFVMASKTLGASKFKLIFKHILPNTLGAIV
                     VTSMFTVPSAIFFEAFLSFIGIGVPAPQTSLGSLVNDGRAMLLIYPHELFIPAMILSL
                     LILFFYLFSDGLRDAFDPKMRK"
     gene            1989..3071
                     /locus_tag="ACGEXS_001510"
     CDS             1989..3071
                     /locus_tag="ACGEXS_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8012523.1"
                     /translation="MTERILEVNDLHVSFDITAGEVQAVRGVDFYLNKGETLAIVGES
                     GSGKSVTTKAITKLFQGDTGRIKKGEILFLGEDLAKKPENELIKLRGKDISMIFQDPM
                     TSLNPTMQIGKQVMEPLIKHKNYSKAQAKKRALEILNLVGLPNAEKRFKAYPHQFSGG
                     QRQRIVIATALACEPKVLIADEPTTALDVTMQAQILDLMKELQQKIDTAIIFITHDLG
                     VVANIADRVAVMYGGQMVETGDVNEIFYDPKHPYTWGLLSSMPDLSTTNDTPLLAIPG
                     APPDLLHPPKGDAFARRSQYALDIDFKVEPPWFKVSPTHFVKSWLLDARAPKVELPEL
                     VKQRMKPMPNNYEKPLKVERVSFNEK"
     gene            3061..4002
                     /locus_tag="ACGEXS_001511"
     CDS             3061..4002
                     /locus_tag="ACGEXS_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFF8012524.1"
                     /translation="MKNDEVLLSIKNLKQYFNAGKKNEVRAIENISFDIYKGETLGLV
                     GESGCGKSTTGKSIIKLNDITSGEILYEGIDIQKIRKRKDLLKFNKKIQMIFQDPYAS
                     LNPRLKVMDIVAEGIDIHHLATDKRDRKKRVYDLLETVGLSKEHANRYPHEFSGGQRQ
                     RIGIARALAVEPEFIIADEPISALDVSIQAQVVNLLLKLQRERGITFLFIAHDLSMVK
                     YISDRIAVMHFGKIVEIGPAEEIYQNPLHDYTKSLLSAIPQPDPESERSRKRFSYIDD
                     EANNHLRQLHEIRPNHFVFSTEEEAAQLRENKLVTQN"
     gene            4021..5676
                     /locus_tag="ACGEXS_001512"
     CDS             4021..5676
                     /locus_tag="ACGEXS_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFF8012525.1"
                     /translation="MTRKFRTLILILIATIALSGCANDDGIYSDKGQVFRKILSSDLT
                     SLDTSLITDEISSEVTAQTFEGLYTLGKGDKPVLGVAKAFPEKSKDGKTLKVKLRSDA
                     KWSNGDKVTAQDFVYAWRKTVDPKTGSEFAYIMGDIKNASDISTGKKPVEQLGIKALN
                     DETLQIELEKPVPYINQLLALNTFAPQNEKVAKKYGKNYGTSADRAVYNGPFKVDDWK
                     QEDKTLLSKNQYYWDKKNVKLDKVNYKVIKDLQAGASLYDTESVDDAVITADQVNKYK
                     DNKGLNFVLTTGTFFVKMNEKQYPDFKNKNLRLAIAQAIDKKGYVDSVKNNGSIPSDT
                     LTAKGIAKAPNGKDYASTMNSPLKYNPKEARAHWDKAKKELGKNEVTFSMNTEDTPDA
                     KISAEYIKSQVEKNLPGVTLKIKQLPFKQRVSLELSNNFEASLSGWSADYPDPMAYLE
                     TMTTGSAQNNTDWGNKEYDQLLKVARTKLALQPNERYENLKKAEEMFLGDAPVAPIYQ
                     KGVAHLTNPQVKGLIYHKFGPNNSLKHVYIDKSIDKETGKKKK"
     gene            5888..7603
                     /gene="opp4A"
                     /locus_tag="ACGEXS_001513"
     CDS             5888..7603
                     /gene="opp4A"
                     /locus_tag="ACGEXS_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFF8012526.1"
                     /translation="MGKLIKYISILLIVVLVLSACGKSSNKDEGVKDATKTETSKHKG
                     GTLNVALTAPPSGVYSSLLNSTHADSVVEGYFNESLLATDKKIRPKAYIASWKDIEPA
                     KKIEFKIKKGIKWHDGNELKIDDWIYSIEVLANKDYEGAYYPSVENIQGAKDYHEGKT
                     DHISGLKKIDDYTMQVTFDKKQENYLTGFITGPLLSKKYLSDVPIKDLAKSDKIRKYP
                     IGIGPYKVKKIVPGEAVQLVKFDDYWQGKPALDKINLKVIDQAQIIKAMEKGDIDVAN
                     DATGAMAKDAKSSNAGLKVLSAPSLDYGLIGFVSHDYDKKANKTGKVRPKYEDKELRK
                     AMLYAIDREKWIKAFFNGYASEINSFVPSMHWIAANPKDLNDYKYDPEKAKKILDKLG
                     YKDRDGDGFREDPKGNKFEINFKHYSGSNPTFEPRTAAIKDFWEKVGLKTNVKLVEFG
                     KYNEDLANASKDMEVYFRSWAGGTDPDPSDLYHTDRPQNEMRTVLPKSDQYLDDALDF
                     EKVGIDEKKRKDIYVKWQKYMNDELPGLPMFQGKSITIVNDKVRNLDIEIGTDQSLYN
                     LTKEA"
     gene            7653..8639
                     /locus_tag="ACGEXS_001514"
     CDS             7653..8639
                     /locus_tag="ACGEXS_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8012527.1"
                     /translation="MNNVLLEVKDLETSLKINNEWLATVENISFELSKGEVLGIVGES
                     GCGKSILSKSIIKLLPEKISKLSNGEVIFDGKRIDTLNEKQLLDIRGNDIAMIFQEPM
                     TALNPVFTIKNQLVESIKSHKKISKKEANKLAKDLLKKVGIARQDEILNSYPHQLSGG
                     MRQRVMIAMAISCSPKLLIADEPTTALDVTIQAQILDLLKELQKETQMAIMMITHDLS
                     VVAEFCDKVLVMYAGQIVEFGGIKEILHNPKHPYTQKLLSTIPKLKEEQKRLETIEGI
                     VPSIQAFHVNKCRFANRCNKKLDICNNQSPKMHVCEDVIVRCHLYKNEYKEI"
     gene            8642..9621
                     /locus_tag="ACGEXS_001515"
                     /pseudo
     CDS             8642..9621
                     /locus_tag="ACGEXS_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499483.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            9614..10576
                     /gene="opp4B"
                     /locus_tag="ACGEXS_001516"
     CDS             9614..10576
                     /gene="opp4B"
                     /locus_tag="ACGEXS_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFF8012528.1"
                     /translation="MIKLILKRLGLMIPLLILISIVVFSLAIIQPGDPFSDLQNGKIK
                     QEAINAQREKLGLNDSIPHQYIRWVNHVIHGDLGESIKYKRPVIDVIEERIPNTILLG
                     AMSLIITYIISFALGITSGRYSYSLTDYTVQIFNYLMLAIPSFIAGVFAIFIFSFELQ
                     WFPFQGSVDINLKEGTFEYYMSKIYHTFLPAFTLGLLSTAGYIQYLRNDIIENSKKDY
                     VLTARSKGLSMNKIYNKHILRNSLIPIITFLGADIVSILGGAVITETIFSYNGIGKLF
                     LESVIGQDYPLMMALTLFFSFLGLLGNLISDITYGFIDPRIRSN"
     gene            10588..11469
                     /gene="opp4C"
                     /locus_tag="ACGEXS_001517"
     CDS             10588..11469
                     /gene="opp4C"
                     /locus_tag="ACGEXS_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFF8012529.1"
                     /translation="MQNKSKSPFKIAFSKFIHNKIAMLSVIFLLIITIVSIIAPLIAP
                     FPVNQQDLLNIKGEMTAQNILGTDSGGRDNFSRLLYAGRISLSIGITSTIGMLLIGIT
                     VGVISGYFGGIVDTLLMRITEFVMLFPFLIFAIVLNAALGDKIKNPYGSAIILVLVII
                     VLSWGGIARLVRGKVLQEKENEYFLAAKSIGTPTYKIILKHLLPNILSVVIVQATLLF
                     AGMIVVESGLSFLGFGISKAIPSWGNMLSDAQEGDVISGKPWIWMPPAIMITLTILSI
                     NFVGEGIKDAFNPRGRR"
     gene            complement(11511..12500)
                     /gene="trpS"
                     /locus_tag="ACGEXS_001518"
     CDS             complement(11511..12500)
                     /gene="trpS"
                     /locus_tag="ACGEXS_001518"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFF8012530.1"
                     /translation="METLFSGIQPSGIPTIGNYIGALKQFVDVQNDYDCYFCIVDQHA
                     ITMPQDRLKLRKQTRQLAAIYLASGIDPDKATLFIQSEVPAHVQAGWMLTTIASVGEL
                     ERMTQYKDKAQKAVEGIPAGLLTYPPLMAADIVLYNTNIVPVGDDQKQHIELTRNLVD
                     RFNSRYNDVLVKPEIRMPKVGGRVMSLQDPTRKMSKSDDNAKNFISLLDEPNVAAKKI
                     KSAVTDSDGIIKFDRDNKPGITNLISIYAGLTDMPIKDIEAKYEGEGYGKFKGDLAEI
                     VKAFLVEFQEKYESFYNSDKLDDILDQGRDKAHKVSFKTVKKMEKAMGLGRKR"
BASE COUNT         4505 a         1764 c         2292 g         3939 t
ORIGIN      
        1 ttatttatat gcttatttct ttatttatta ttattacaat tacatttttc ttaatgaaat
       61 taatgccagg ttcgccattt aacgatgcta aattaaatgc tgaacaaaaa gaaattttaa
      121 atgaaaaata tggattaaat gatcctgtag ctacgcagta tttacattat ttaaaaaatg
      181 ttgttacagg cgattttggt aattcattcc agtatcataa tcaacctgtg tgggatttga
      241 ttaaaccgag actactacct tcttttgaaa tgggtcttac agcaatgttc atcggtgtga
      301 tactgggact tattttaggt gttgcagcag ctactaaaca aaattcttgg gttgactata
      361 caactacagt tatttcagtt attgcagtat ctgtaccatc ttttgtactt gctgtacttt
      421 tacaatatgt atttgcagtt aaattaagat ggttcccagt agctggatgg gaaggttttt
      481 cgaccgcggt attaccgtca cttgcattat ctgcagctgt tttagcaact gtcgccagat
      541 acataagagc agagatgata gaggtattaa gttcagacta tattttatta gcgagagcta
      601 aaggtaattc gacaatgcgt gtactttttg gacatgcact tagaaatgct ttaattccaa
      661 ttattacaat tatcgttccc atgttagcaa gtattttaac aggcacttta acaattgaaa
      721 atatttttgg agttcctgga ttaggggatc aattcgtacg ttcaattaca acaaatgatt
      781 tctcagtaat catggcaatc acactattat ttagcacact gtttatcgtt tctattttta
      841 ttgtagatat tttgtacggt gtgatagatc cacgaattcg tgttcaagga ggtaaaaaat
      901 aatggctgaa aataaaaaca atttgtcgat taacgacgat cattctaatg cagctatgac
      961 gcatacctct gacgctatcg catcatctga ttttattatt agagaattag atttgaatca
     1021 ggaacctgaa atgcaacgag aaagcaaaaa cttttggcaa gatgcttggg ctcagttaaa
     1081 acgaaataag ttagctgttg tcggtatgat aggtttaatt atcattgtaa tatttgcttt
     1141 tatcggtcca gttataaata aacatgatta tgctgaacaa aatgtagaac atagaaatct
     1201 tccggcaaaa atacctgtat tagacaaagt tccattttta ccttttgatg gtaaagatgc
     1261 agatggcaag gatgcttata aagcagcaaa tgctaaagaa aattattggt ttggtactga
     1321 tcagttgggt cgagatttat ggacaagaac atggaaaggt gctcaaattt cattgtttat
     1381 cggtgttgtt gcagcgatgt tagatatttt tattggtgtt gtatatggtg cgatttctgg
     1441 attcttcggt ggacgtgtcg atacgattat gcaacgtata cttgaagtca tagcatctat
     1501 tccgaattta attgtcgtaa ttttatttgt attaattttt gaaccatcca tttggacaat
     1561 tatattggct atgtctatca caggctggtt aggcatgagc agagttgtac gtggagaatt
     1621 tttaaaatta aaaaatcaag agtttgtcat ggcttcgaaa acattggggg cttcaaaatt
     1681 caaattgata tttaagcata ttttacctaa tacattaggt gctatcgtgg ttacatcaat
     1741 gtttacagta cctagtgcta ttttcttcga agcattttta agtttcattg gtataggtgt
     1801 acccgcacct caaacatcgt tagggtcatt agtaaatgat gggcgcgcaa tgttattaat
     1861 ttatccacat gaattattta taccagcaat gattttaagt ttattaattc tattctttta
     1921 cttatttagt gatggattac gtgatgcatt tgatccgaaa atgcgtaaat aaaaaggggg
     1981 catagcatat gactgaaaga atattagaag taaatgattt gcatgtttcc tttgatatta
     2041 cagcagggga agtgcaggca gtgagaggcg tagattttta tttgaacaaa ggggaaacat
     2101 tggcaattgt tggtgaatca ggttcaggta aatctgtaac aacaaaagca attacaaaat
     2161 tattccaagg ggacacagga agaattaaaa agggagaaat tttattttta ggggaagatt
     2221 tagcaaaaaa acctgaaaat gagttgatta aattacgtgg caaagatatt tcaatgatct
     2281 ttcaagatcc aatgacatct ttaaacccaa cgatgcaaat tggtaaacaa gtcatggaac
     2341 cattaattaa gcacaaaaat tatagtaaag cacaagctaa aaagcgcgca ttggaaatac
     2401 taaatcttgt aggtttacca aatgcagaaa aaagatttaa agcatatcct catcaatttt
     2461 caggtggaca aaggcaaaga attgttattg caaccgcatt agcttgtgaa cctaaagtgc
     2521 tcattgctga tgaaccaacg actgcattag acgtaacgat gcaggcacaa attttagatt
     2581 taatgaaaga actacaacaa aaaatcgata cagcaattat ttttataacg catgatttag
     2641 gggttgttgc gaatattgct gatagagtgg cagttatgta tggtggtcaa atggttgaaa
     2701 caggagatgt taacgaaata ttttatgatc caaagcatcc atatacatgg ggattattat
     2761 cgtcaatgcc tgatttatca acaacaaatg acacaccatt actagcgatt cctggagcgc
     2821 cacctgattt attacaccca cctaaaggtg atgcatttgc gagacgtagt caatatgcat
     2881 tagatattga ttttaaagta gaaccaccgt ggtttaaagt ttcaccgaca cattttgtga
     2941 aatcttggtt attagacgca cgtgcaccaa aagttgaact acccgagctg gtaaaacaac
     3001 gtatgaaacc gatgcctaat aattatgaaa aaccactcaa ggtagaaagg gtgtcgttca
     3061 atgaaaaatg atgaagtgct attatctatt aaaaatttaa agcaatattt taacgcagga
     3121 aagaaaaacg aagtgagagc gattgaaaat atttcgtttg atatatacaa aggggaaaca
     3181 ttaggtttag taggagaatc ggggtgtggt aaatctacaa ctggtaaatc aattattaaa
     3241 cttaatgata ttacaagtgg agaaattttg tatgagggta ttgatataca aaagattcgt
     3301 aaacgtaaag atttgcttaa atttaataaa aagatacaga tgatttttca agacccatat
     3361 gcgtctttaa atcctaggtt aaaagtaatg gatatagtag ctgaaggtat tgatatccat
     3421 catttagcaa ctgataagcg tgaccgaaaa aaacgtgtct atgatttact tgaaactgtt
     3481 ggattaagta aagaacatgc caatcgctat cctcatgaat tttcaggtgg acaacgccaa
     3541 cgtattggaa ttgcccgtgc attagccgtt gaaccagaat tcattatcgc ggacgaacca
     3601 atatcggcat tggatgtttc aatccaagct caagtagtta atttattatt aaaattacaa
     3661 cgtgaaagag ggattacgtt cctatttata gctcatgatc tatcaatggt gaagtatatt
     3721 tcagatcgta ttgcagtcat gcattttggg aaaatagttg aaattggacc ggcagaagaa
     3781 atttatcaaa atccattaca cgattatact aagtctttat tatcagccat tccacaacct
     3841 gatcctgaat cagaacgcag tcgcaaacga tttagttata ttgatgatga agcaaataat
     3901 catttaagac aattacatga aattagaccg aatcactttg tctttagtac tgaagaagaa
     3961 gcggcacaac tacgagaaaa taaattggtg acacaaaatt aaggggaagg gggaaatgca
     4021 atgacgagaa aatttagaac acttatttta attttgattg ctacaattgc attaagtggt
     4081 tgtgctaatg acgatggtat ttattcagat aaaggtcaag tattcagaaa aattttgtca
     4141 tcagacttaa catcccttga tacatcatta ataacggatg aaatatcttc tgaagtgact
     4201 gcgcaaacat tcgaaggttt atacacatta ggaaaaggtg acaaaccggt gttaggtgtt
     4261 gcgaaagctt ttcctgaaaa gagtaaagat ggtaaaactt taaaggttaa attaagaagc
     4321 gatgctaaat ggagcaatgg tgacaaagtg actgcacaag actttgttta tgcttggaga
     4381 aaaacagttg accctaaaac aggttctgaa tttgcataca ttatggggga cattaaaaat
     4441 gcgagtgata ttagtactgg taagaaacct gtagagcaat taggtatcaa agcattaaat
     4501 gatgaaacat tacaaattga attagaaaag ccggttccat atattaatca attattagca
     4561 cttaatacct ttgcacctca aaatgaaaaa gttgccaaaa aatatggtaa aaattacggt
     4621 acgtcagctg atagagcggt atacaatggt ccatttaaag ttgatgattg gaaacaagaa
     4681 gataaaacct tactatctaa aaatcagtat tattgggata aaaagaatgt aaaattagat
     4741 aaagtgaatt ataaagttat taaagactta caagccggtg catcattgta tgatactgaa
     4801 tcagtagatg acgcagttat tactgcagat caagtaaata aatataaaga caacaaagga
     4861 ttaaactttg tgttaacgac tgggacattt tttgtaaaaa tgaatgaaaa acaatatcct
     4921 gattttaaaa acaaaaattt aagattggct atcgcacaag caatagataa aaaaggatac
     4981 gttgattcag tgaaaaacaa tggctcaatt ccttccgata cactaacagc caaaggaatt
     5041 gcgaaagcgc ctaatggcaa agattatgcg agtaccatga attcgccttt aaaatataat
     5101 cctaaagaag caagagcaca ctgggacaaa gctaaaaaag agttaggtaa aaatgaagtg
     5161 acattttcaa tgaacacaga agatacgcca gatgcaaaaa tatctgctga atatatcaaa
     5221 tcgcaagttg agaaaaattt accaggagtt actttgaaaa ttaagcaatt accgtttaaa
     5281 caaagagtat cactagaact gagtaacaat tttgaagcat cacttagtgg ttggtctgca
     5341 gattaccctg atcctatggc ttatttagaa acaatgacca caggtagcgc acaaaataat
     5401 acagactggg gtaataaaga atatgatcaa ttacttaaag tagcaagaac caaattggca
     5461 cttcaaccga acgaacgata tgaaaacttg aaaaaagcag aagaaatgtt cctaggagat
     5521 gcaccggtag caccaattta tcaaaaaggt gttgcacatt taacaaatcc tcaagtaaaa
     5581 ggattaattt accataaatt tggtccaaat aactcactta aacatgtata tattgataaa
     5641 tcgatagata aagaaacagg taagaagaaa aaataatatg ctttgtaaat taggctggag
     5701 acatatctcc agtctttttg tgttggataa aaactttggg aataaaaatt taaaataagt
     5761 cgttttttaa attactgaaa ttgattaaat gcataaataa ctgaatattc taaaaataaa
     5821 cttgtaataa ttttttctat gagtaaacta aaaagaaaaa attagattga aagtaggagg
     5881 catatgtatg gggaagctaa ttaaatatat ttcaatactt cttattgtcg ttttagtgtt
     5941 gagtgcttgc ggaaaaagca gtaataaaga tgaaggagta aaagatgcta ctaaaacgga
     6001 aacctcaaaa cataaaggtg gtaccttaaa tgtagcatta acagcaccgc caagtggtgt
     6061 ttattcttcg ttattaaata gtacacatgc agattctgta gttgagggat attttaacga
     6121 aagcttatta gcaactgata aaaaaatacg tcctaaggca tatattgctt catggaagga
     6181 catcgagccg gctaagaaaa tagaatttaa aattaaaaaa ggtattaaat ggcatgatgg
     6241 taatgaattg aaaattgatg attggattta ttcaattgaa gtcttagcta acaaggacta
     6301 cgaaggtgct tattatccaa gtgtagaaaa tatccaaggt gcgaaagatt atcatgaagg
     6361 aaaaactgat catattagcg gattgaagaa aatagatgac tacactatgc aggttacatt
     6421 tgataaaaaa caagaaaatt acttaacagg atttattact ggacctttat taagtaaaaa
     6481 atatttatca gatgtaccaa ttaaagattt agcgaaatca gataaaatcc gaaaatatcc
     6541 tattggtatt ggaccgtata aagttaagaa aatcgttcca ggtgaggctg ttcaactcgt
     6601 taaatttgat gattattggc aaggtaagcc tgcactagac aaaatcaatt taaaagttat
     6661 tgatcaagcg caaattatta aggcaatgga aaaaggcgat attgatgttg cgaatgatgc
     6721 taccggtgca atggcaaaag atgctaagtc atctaatgct ggtctcaagg tattatctgc
     6781 gccaagctta gactacggtt taataggatt cgtatctcat gattacgata aaaaagctaa
     6841 taaaactggt aaagtgagac caaaatatga agacaaagaa ttacgtaaag caatgcttta
     6901 tgcaattgat agagaaaaat ggatcaaagc gtttttcaat ggttacgcta gtgaaatcaa
     6961 tagttttgta ccatctatgc attggatagc agccaatcct aaggacctaa atgattacaa
     7021 atatgatcct gaaaaagcta aaaaaatctt agataagtta ggttataaag atagagatgg
     7081 tgacggattt agagaagatc ctaaaggtaa taaatttgag attaacttta aacattattc
     7141 aggttctaat cctacttttg aaccaagaac tgctgcgata aaagatttct gggaaaaagt
     7201 tggcttgaaa acaaatgtga agttagtaga attcggtaaa tataatgaag acttagcaaa
     7261 tgcatctaaa gatatggaag tgtacttcag atcatgggca ggaggtacag atccagatcc
     7321 atcagattta taccacactg atagacctca aaatgaaatg agaacagttt taccaaaatc
     7381 agatcaatat ttagatgatg cattagactt cgaaaaagta ggcattgatg aaaagaaacg
     7441 taaagatatt tatgttaaat ggcaaaaata tatgaatgat gagttacctg gattaccaat
     7501 gttccaaggt aaatcgataa ctattgttaa cgataaagta cgaaacttag acattgaaat
     7561 tggaactgat caaagtttat ataatttaac taaagaagct tagtaatgta acctcgacga
     7621 ttttgtcgag gtttacttat atgggggatg ttatgaataa tgtattgtta gaggttaaag
     7681 atttagaaac atcattaaaa ataaataatg aatggttagc aactgttgaa aatatttctt
     7741 ttgaattatc taaaggagaa gttttgggta tagtagggga atctggttgc ggtaagtcca
     7801 tattaagtaa gtcaattatt aaattattac cagaaaagat atctaaacta agtaatggag
     7861 aagttatatt tgatggtaaa cgaatcgata cgctcaatga gaagcaattg ctagatattc
     7921 gaggaaatga tattgctatg atttttcaag aacctatgac tgctttaaat cctgtattta
     7981 ccataaaaaa tcaacttgtg gaatctataa aatcacataa aaaaatttct aaaaaagaag
     8041 caaataaatt agcaaaagat ttactaaaaa aagttggaat tgctagacaa gatgaaatat
     8101 taaatagcta tcctcatcaa ttatctggtg gtatgagaca aagagtaatg attgcaatgg
     8161 ccatttcatg ttctcctaaa ttattaattg ctgatgaacc tacaacagca ttggatgtca
     8221 cgattcaagc gcaaatatta gacttattaa aagaattgca aaaggaaacg caaatggcaa
     8281 ttatgatgat tacacatgat ttgagtgtag ttgctgagtt ttgcgataaa gtcttagtta
     8341 tgtatgcagg tcaaattgta gaatttggag gcataaaaga aatactacac aatccgaaac
     8401 atccttatac ccaaaaatta ttatcaacaa ttccaaaact taaagaagag cagaaacgac
     8461 ttgaaacgat agaaggaatt gtgccatcaa tccaagcatt tcacgttaat aagtgcagat
     8521 ttgcaaatag atgtaacaaa aaactggata tttgtaataa tcaatctcct aaaatgcatg
     8581 tttgtgaaga cgtcattgta cgttgtcatt tgtacaaaaa tgaatataag gagatataag
     8641 aatggaaaat attttagaag tcaaccaaat aaaaaaatac tacaaaatta aaactggatt
     8701 attacaaaaa actcagtacg ttaaagctgt tgatgacgta tcgttttcaa taaaaaaagg
     8761 acaaactttt ggattagtag gagaatcggg ttgtggtaag tcaacgttag gtaaagtgat
     8821 tatcaggctt gaagatgcaa cttcaggctc aataattgtt aatggtgaag atataacaag
     8881 attacaaggt aaaaaactca gaaaatcacg acaacaatat cagatgatat ttcaagatcc
     8941 gtatgcatca ttgaatccga tgcaaatggt tggagatatc atttcagaac ctattttaaa
     9001 ttataaaaaa ttgccaaaag aagaaataaa aaagaagtac tatatttatt aaaatgtgtt
     9061 ggcctaagtg aagatgcata ttataaatat gcacatgaat tttcaggtgg acagagacaa
     9121 agagtgggaa ttgcaagagc attggctttg cgtccgagtt taattgttgc tgatgagcct
     9181 gtaagtgcat tagatgtatc tgttcaatct caagtactga atttattaaa agatttacaa
     9241 gaacaattta acttaagcta tttatttatc gcacatgatt taagtgtagt aaaacatata
     9301 agtgatgtca ttggagttat gtatttaggt catatagttg aaatcgcatc tgataaagaa
     9361 atttatgaaa atcccaaaca tccatataca aaagcgttga tttcatcaat accacaaatt
     9421 gataaacata ataacaatag aattatatta aaaggagaat taccttcgcc aagtaatccg
     9481 ccgcaaggtt gtccttttca tacaagatgt ccaattgctc aagatatgtg caaaaaaagt
     9541 atgccagaat taaaggacat tggtaatgaa catcaagttg cttgtttcta cgtagataaa
     9601 gtaggtgatt taaatgatta aattaatact taaaagatta gggttaatga tcccgttact
     9661 aattttaatc tcaatagttg tattttcatt agcaatcata caaccaggag atccattttc
     9721 agatctacaa aacggtaaaa taaaacaaga agcaataaat gcacaaaggg aaaaattagg
     9781 tctcaacgac tcaataccac atcaatacat tagatgggtc aatcatgtta tacacggtga
     9841 tttaggggag tcaatcaaat ataaaaggcc ggtaattgat gttattgagg aaagaattcc
     9901 aaatacaata ttactcggtg ctatgtcatt aattattact tatattatct catttgcttt
     9961 aggaataacg tcaggtagat attcttacag tttgacggat tatactgtgc aaatatttaa
    10021 ttatttgatg ttagccattc catcttttat tgcgggagta tttgcaattt ttattttttc
    10081 ttttgaatta caatggttcc cgtttcaagg ttctgttgat attaacctta aagaaggtac
    10141 ttttgaatat tatatgagta aaatatatca cacattttta cctgcattca ctttaggttt
    10201 attatctact gctggttata ttcaatattt acgtaatgat attattgaaa attctaaaaa
    10261 agattatgta ttgacggcaa ggtcaaaagg attatctatg aataaaattt ataataaaca
    10321 tatattgaga aattctctaa tacctattat tacattttta ggtgctgata ttgtaagtat
    10381 tttaggtgga gccgtgatta cggagactat cttttcatat aacggtatcg gtaaattatt
    10441 tttagaatcg gtaataggcc aagactatcc attaatgatg gcattaacgt tatttttctc
    10501 atttttaggt ttactgggta atttgatttc tgatattact tatggattta tagatccaag
    10561 aattagaagt aactaggggg gattagaatg caaaataagt caaaatcgcc ttttaaaatt
    10621 gcattttcta aatttattca taataaaatt gcaatgttat cggttatttt tttattaatc
    10681 ataactattg tatcaattat agcgccatta atagctcctt ttccagtgaa ccaacaagat
    10741 ttattaaata taaaaggtga aatgacagca caaaacattc ttggtacaga ctctggtggt
    10801 agggataact ttagtcgttt gttatatgca ggtcgtattt cattatccat tggaattaca
    10861 tctacaatag gaatgctttt gattggaatt acagttggag tgatttctgg ttattttgga
    10921 ggtattgttg atacattatt aatgagaata accgaatttg ttatgttatt tccattttta
    10981 atatttgcaa ttgtattaaa tgctgcactt ggagataaaa ttaaaaatcc ttatggatct
    11041 gccataattc ttgttctagt tattatcgta ttaagttggg gaggtattgc aagacttgtt
    11101 cgtggtaaag tacttcaaga aaaagaaaat gaatactttt tggcagcaaa atcaattggt
    11161 acacccacat ataaaattat tttgaaacat cttttgccga atatattaag tgtagttatc
    11221 gtacaagcaa cattgttatt tgccggtatg attgtagtgg aatcaggatt gagcttttta
    11281 ggattcggaa ttagtaaagc aataccatct tggggtaata tgttgagtga tgctcaagaa
    11341 ggggatgtta taagtggtaa accgtggata tggatgccac ctgctataat gattacatta
    11401 actatattaa gtataaactt tgtaggggaa gggattaaag atgcttttaa tcctagaggt
    11461 agacgttaaa taataaaaga ggcactagtt aattctagta cctctttatt ttatctctta
    11521 cgtcctaaac ccatcgcttt ttccattttt ttgacagttt taaatgaaac tttgtgtgct
    11581 ttatctctac cttgatctaa aatatcatca agtttatctg agttatagaa actttcgtat
    11641 ttttcttgga attctactaa aaatgcttta actatttcag caaggtcacc tttaaattta
    11701 ccataacctt cgccctcata ttttgcctca atatctttaa ttggcatgtc tgttaatcca
    11761 gcgtatattg aaattaaatt tgttatacct ggcttgttgt cacgatcaaa tttaataata
    11821 ccatctgaat cagttactgc gcttttaatt tttttagctg caacattcgg ctcgtctaat
    11881 aatgaaatga agtttttagc attatcatca ctcttactca tttttcttgt tgggtcttgt
    11941 aaactcatga cacgtccacc aactttaggc atacgaattt caggtttcac aagcacatca
    12001 ttatagcgac tattaaatct atctacaagg ttacgagtca attcgatatg ctgcttttgg
    12061 tcatctccaa ctggaacgat attagtattg taaagaacaa tatcagctgc cattaaaggt
    12121 ggatatgtta atagaccagc aggtatacct tcaactgctt tctgagcttt atctttgtat
    12181 tgcgtcatac gctctaattc tccaacagaa gcaatcgtag ttaacatcca tcctgcttgt
    12241 acgtgtgcag ggacttcaga ttgtatgaac aatgttgctt tgtctggatc tataccagaa
    12301 gctaaataaa tcgctgctaa ttgtctggtc tgtttacgta attttaaacg atcttgtggc
    12361 attgtaattg catgttgatc tacgatacag aaataacaat catagtcatt ttgcacatca
    12421 acaaattgtt ttagtgcgcc aatataattt ccaatagtag gaattccact aggttggatg
    12481 cctgaaaata atgtctccat
//
LOCUS       DAXWFK010000073        12466 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.073, whole genome shotgun sequence.
ACCESSION   DAXWFK010000073 DAXWFK010000000
VERSION     DAXWFK010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12466
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.073"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..1161
                     /locus_tag="ACGEXS_001519"
     CDS             <1..1161
                     /locus_tag="ACGEXS_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HFF8012531.1"
                     /translation="FKNMRLSWQITKRNKFRLVIEIVILELIIGAILTLIISGATYLA
                     ICVDEEGDKFLVSSILFVVLKSALFFYYLFTKLSLISVLVLHLKQENVLDQPGLEFKY
                     PKPKRKSRFFIISMVLAVTCFIGYNMYLLYNNTINTNISIIGHRGFEDKGVENSIPSL
                     KAAAKANVEYVELDTIMTKDKQFVVSHDNNLKRLTGVNKNISESNFKDIVGLKMRQNG
                     HEAKFVSLDEFIETAKQSNVKLLVELKPHGKEPADYTQRVIDILKKHGVEHQYRVMSL
                     DYDVMTKLKKEAPYLKCGYIIPLQFGHFKETSLDFFVIEDFSYSPRLVNQAHLENKEV
                     YTWTINGEEDLTKYLQTNVDGIITDDPALADQIKEEKKDETYFDRSIRILFE"
     gene            complement(1325..1669)
                     /locus_tag="ACGEXS_001520"
     CDS             complement(1325..1669)
                     /locus_tag="ACGEXS_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN family protein"
                     /protein_id="HFF8012532.1"
                     /translation="MTTQMKIKTYLVAGIKAALLDTTGIKLASKSETTSHTYQHQALV
                     DQLHELIANTDLNKLSYLNLDAFQKRDILAAHYIAKSAIRTKNLDQMTKAKQRLESIY
                     NSISNPLHSQNN"
     gene            1859..3526
                     /gene="coa"
                     /locus_tag="ACGEXS_001521"
     CDS             1859..3526
                     /gene="coa"
                     /locus_tag="ACGEXS_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylocoagulase"
                     /protein_id="HFF8012533.1"
                     /translation="MKKQIISLGALAVASSLFTWDNKADAIVTKDYSGKSQVNAGSKN
                     GTLIDSRYLNSALYYLEDYIIYAIGLTNKYEYGDNIYKEAKDRLLEKVLREDQYLLER
                     KKSQYEDYKQWYANYKKENPRTDLKMANFHKYNLEELSMKEYNELQDALKRALDDFHR
                     EVKDIKDKNSDLKTFNAAEEDKATKEVYDLVSEIDTLVVSYYGDKDYGEHAKELRAKL
                     DLILGDTDNPHKITNERIKKEMIDDLNSIIDDFFMETKQNRPKSITKYNPTTHNYKTN
                     SDNKPNFDKLVEETKKAVKEADDSWKKKTVKKYGETETKSPVVKEEKKVEEPQAPKVD
                     NQQEVKTTAGKAEETTQPVAQPLVKIPQGTITGEIVKGPEYPTMENKTVQGEIVQGPD
                     FLTMEQSGPSLSNNYTNPPLTNPILEGLEGSSSKLEIKPQGTESTLKGTQGESSDIEV
                     KPQATETTEASQYGPRPQFNKTPKYVKYRDAGTGIREYNDGTFGYEARPRFNKPSETN
                     AYNVTTHANGQVSYGARPTYKKPSETNAYNVTTHADGTATYGPRVTK"
     gene            complement(3708..3815)
                     /locus_tag="ACGEXS_001522"
     CDS             complement(3708..3815)
                     /locus_tag="ACGEXS_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012534.1"
                     /translation="MCVRTRLVSSSSARLSKAIIIAVIVVYHLDVRGLF"
     gene            complement(4129..5313)
                     /locus_tag="ACGEXS_001523"
     CDS             complement(4129..5313)
                     /locus_tag="ACGEXS_001523"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HFF8012535.1"
                     /translation="MQEAYIVAYGRSAAAKAKQGALFHERPDDVAAKVLQGVLKRIDG
                     KFNKNMIEDVIVGTAFPEGLQGQNIARTIALRAGLSDTVPGQTVNRYCSSGLQTIAIA
                     ANQIMPGQGDILVAGGVELMSAVPMGGNEPTNNPTLQYDDIGASYPMGLTAENVASQF
                     DVSREDQDAYAVRSHQRAYDAQRDGRFKDEIIPIQVNSVEYTNAGPKVHTNIFDQDEF
                     IRPDTTMEALAKLRTVFKADGTVTAGTSAPLSDGAGFVVLMSGDKVKELGVTPIARFV
                     GFKAVGVDPKIMGIGPAYAIPEVLSLSNLSVEDIDLIELNEAFASQTIASIKEVGLDI
                     SRTNVNGGAIALGHPLGATGAMLTARLLNEMGRRPDSRYGMVTMCIGVGMGAAAIFEY
                     VR"
     gene            complement(5343..7604)
                     /locus_tag="ACGEXS_001524"
     CDS             complement(5343..7604)
                     /locus_tag="ACGEXS_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HFF8012536.1"
                     /translation="MTINKVTVLGAGTMGAQLAALFVNAGLKVKLLDIVVDKNDPNLI
                     AKKSYDKITDKKRPLLFDLNLASHLTYGNFDDDLVNDDADLYIEAVKEDIEIKHAVWQ
                     QVLQHAKEDALFATNTSGIPINAIAQAFNEKDQERFFGLHFFNPPRIMKLVELIPTSH
                     TKESIILDVKNFAQNVLGKGVIVVNDVPGFVANRVGTQTMNDIMYRAEQHKISIVDVD
                     ALTGQAIGRPKTGTYALSDLVGLDIAVSVIKGMQQVPEETPYFHDVKIVNTLFDNGAL
                     GRKTKQGFYKKDKETKARLVYDVEKQDYVPVSQPQLPILNEFNKDLVHNLDTIFNAQD
                     EAGLFLWETLRNNFYYSAINVPKATDDFRDIDRALVWGFNWKLGPFQLWDAMGYERVK
                     TRMEDELGDLPQWISDLDGGFYKQDETIEYATPISHFVKDELWDKGDAKLSVTHDDQL
                     LLKLQSKNNVITDEFNDALVDAIDLLENDHYTSMVIYADGNNFSVGANLFLMKKAHED
                     GLVDDVVAQSIDKLHYSFNRLKYSLKPVVTAVQGRALGGGCELVLYSPIVVAASETYI
                     GLVEAGVGLLPSGGGLAEMADRILRTSHKFDDKQASMTKVLTNIAFAKVSTNAFEARR
                     YGYLRDTDTIIFNTAQRVEVALKRAKYEAETNYIPNPRHQYIALGEDFKALIQGQLDA
                     QRRGHFISDHDYHIALNIATILAGGDLPRNTFINQRYIQSLEKIGFIDLLKSKKSYER
                     IAHMLKTGKPLRN"
     gene            complement(7790..9001)
                     /locus_tag="ACGEXS_001525"
     CDS             complement(7790..9001)
                     /locus_tag="ACGEXS_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HFF8012537.1"
                     /translation="MTFEKETVLKTLFPEDVLSIAKGLTDGEVEFLQQVDSLLESKYR
                     ENINQHWIDATVPEDYFKDLGELNYFNNPLLYKDRPNAKMPSQLFQFFMSYLLARFDI
                     SLATLLGVHQGLGHNTFYFGGSKEQIAKYVPKLQSHELRTCFALTEPEHGSDVAGGLE
                     TVAERQGDTWVINGEKKWIGGAHVSDVIPVFAVNKETGKPHCFVVRPEQDGVDIEVID
                     NKIALRIVPNALIKLTNVKVDEADRLQNITSFKDIAKILYSTRAGVAYMATGGMAGAL
                     RATLDYVTERKQFGKPISKYQLIQEKLAMMQGNLAQAMATCAQLANMQAHGEYDEVAT
                     STAKMMNALRLRETVAMGRGITGGNGILADDYDIARFFSDAEAIYTYEGTHEINALVI
                     GRALTGDSAFV"
     gene            complement(9113..10618)
                     /locus_tag="ACGEXS_001526"
     CDS             complement(9113..10618)
                     /locus_tag="ACGEXS_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HFF8012538.1"
                     /translation="MNFDWIKTRSDFDDDKPAVIDHAKQTSWTYQQLNARADNMAHYL
                     TSQGVKKGDVIGIFAPNDIAILDLLFACFKTGAVFLPLNWRLNPKEIAAIVEDAQLKL
                     LFYAEKHLSSLTDIDQNLLHMDIDVAQYDEIVNPDYHQPFQATPVEPQDLAALIYTSG
                     TTGSPKGVMFSYESFVHNGANLELTYKFNSNYITIVSTPMFHVLGFNDTVLPVLMSGG
                     TLILQRYFNGEELNDMIAQYHPTFIIMIPTMYYSTLRASNFNPENFRAMDYIIQGGSQ
                     PLPSIQAAFKQYGINIINGYGLTEAPLVLVNTPENSKRKPMSIGKAVMFVDARILDDN
                     GEEVPTGEIGELAIKAKNVTPGYWNKPAETAKAFHGRYLLTGDLAKMDNDGDIFIIDR
                     KKELIITGGENVLPSEVENALAEHPLVDRCVVVGYDHPKYGESIAAAIILREDEPHYA
                     EILNQHMRSRLAGYKVPRMYVPVTHMPLNSTQKPDKLAIRQMMNDKVSQTL"
     gene            complement(10644..12221)
                     /locus_tag="ACGEXS_001527"
     CDS             complement(10644..12221)
                     /locus_tag="ACGEXS_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFF8012539.1"
                     /translation="MEVTCLKQITWHDLQHIIKDGDVIGLPALAVANLPAEVLRAVLA
                     QHDTYHTPNDLTFILANDIHSLGAAPDLDDFIERRMIKRVIMSILTASSKTAQAMKNN
                     DIEAYFLPQGIIATHYRQSNQLLPGVITKIGLNTAVDPRYGGGKVNTRTTDDLVSLVT
                     INDETYLHYTFPSVDVALLRGTYADQQGNIYLTQEAYLSECYHVALNAKANHGKVIVQ
                     VKALVDDYQLKPNEVVIPGNLVDYVYVTEDEKNHRQVIQSHYLPALSGEERIDGIPEP
                     ALPFNSRKLILRRAAQFLTYGDTISIGYGINNELSNLLHEECVEHDVQPILDVGIFGG
                     FVGSREHFGMNYNADVRMPHDRAWDFIYNNGVSVAYLSFAEVDQYGNVNVSYFNDRLN
                     GCGGFIDITQSVNKIIFSGTFVAGSHVSCHNQRLNIETEGQNQKFVSDVSHIDFNAQY
                     SQSLEQEIYFVTDRAVFELTNQGLKLIEIAPGLDLHKDILNQMAFKPIIADHLKLIDT
                     SIYKEKWGQLKQSIHKV"
BASE COUNT         4039 a         2273 c         2171 g         3983 t
ORIGIN      
        1 tttaaaaata tgagactaag ttggcaaatt acgaagcgaa ataagtttcg gcttgttata
       61 gaaatagtta tattagaact catcattggt gcgattttaa cattaattat ttcaggagca
      121 acatatcttg ctatttgtgt agatgaagaa ggagataagt ttttagtctc atcaatttta
      181 tttgttgtat tgaaaagcgc attgttcttc tattatttat ttacgaaatt atcattaatc
      241 agtgtgttag tactgcactt aaaacaagag aatgtattag accaaccggg cttagaattt
      301 aaatatccaa aaccgaaacg gaagtctagg ttctttataa tttcaatggt gcttgcagtg
      361 acatgtttta tcggttataa catgtactta ctttacaata atactatcaa tacaaatatc
      421 tccattattg gccatcgtgg tttcgaagat aaaggcgttg aaaattctat tccgtcattg
      481 aaagctgctg caaaagcgaa tgtcgaatac gttgagttag atacaattat gacgaaagat
      541 aaacaatttg ttgttagtca tgataacaat ttgaaacgtt taacaggtgt taataaaaat
      601 atttctgaat ctaatttcaa agatatcgtc ggtttgaaaa tgcgtcaaaa tggacatgaa
      661 gcaaaatttg tatccttaga cgaatttatt gaaacggcta aacaatcaaa tgtgaagcta
      721 ctagtagagt taaagccaca tggtaaagaa ccagcagatt atacacaacg tgttattgat
      781 attttgaaaa agcatggtgt tgaacatcaa tatcgtgtga tgtctttgga ttatgatgtg
      841 atgactaagt tgaaaaaaga agcgccatat ctcaagtgtg gttatatcat tccgttgcag
      901 tttggtcatt ttaaagaaac atcattagat ttctttgtca tcgaagattt ttcttattcg
      961 ccaagacttg ttaatcaagc gcacttggaa aataaagaag tctatacttg gactattaac
     1021 ggcgaagaag atttaacgaa atacttacaa accaatgttg atggtattat cacagatgac
     1081 ccagcattag ctgatcagat taaagaagaa aagaaagacg aaacatactt cgatcgttct
     1141 ataagaattt tgtttgaata atataaacaa agacctctaa agttatcaag atgatacctt
     1201 cagaggtctt tttaatgttg ccatctatgg gataggcaat cgtttcattc gtttatattc
     1261 atatgacaag tatttgtatg gcaatttggc gtcacaaaca cttacatgat ttattggtga
     1321 attattaatt gttttgtgaa tgcaaagggt tagaaattga attgtaaata ctttctaatc
     1381 tttgtttcgc tttagtcatt tgatccaaat ttttagtgcg tatagcggat tttgcaatat
     1441 agtgcgcagc taaaatatcg cgtttttgaa acgcatctaa atttaggtac gataatttat
     1501 ttaagtcagt gtttgctatt aattcatgta attgatctac aagcgcttga tgttgatacg
     1561 tatgtgatgt agtttcagat ttgcttgcta atttaatacc agtcgtatca aggagcgccg
     1621 ctttaatacc agcaactaaa tatgttttga ttttcatttg tgttgtcatg ctttgttact
     1681 cctttgatgt acattaatca aaaaaattat acactattgt atattgcaaa gctaattaac
     1741 tataacaaaa agatagttaa tgctttgttt attctagtta atatatagtt aatgtctttt
     1801 aatattttgt ttctttaatg tagattgggc aattacattt tggaggaatt aaaaaattat
     1861 gaaaaagcaa ataatttcgc taggcgcatt agcagttgca tctagcttat ttacatggga
     1921 taacaaagca gatgcgatag taacaaagga ttatagtggg aaatcacaag ttaatgctgg
     1981 gagtaaaaat gggacattaa tagatagcag atatttaaat tcagctctat attatttgga
     2041 agactatata atttatgcta taggattaac taataaatat gaatatggag ataatattta
     2101 taaagaagct aaagataggt tgttggaaaa ggtattaagg gaagatcaat atcttttgga
     2161 gagaaagaaa tctcaatatg aagattataa acaatggtat gcaaattata aaaaagaaaa
     2221 tcctcgtaca gatttaaaaa tggctaattt tcataaatat aatttagaag aactttcgat
     2281 gaaagaatac aatgaactac aggatgcatt aaagagagca ctggatgatt ttcacagaga
     2341 agttaaagat attaaggata agaattcaga cttgaaaact tttaatgcag cagaagaaga
     2401 taaagcaact aaggaagtat acgatctcgt atctgaaatt gatacattag ttgtatcata
     2461 ttatggtgat aaggattatg gggagcacgc gaaagagtta cgagcaaaac tggacttaat
     2521 ccttggagat acagacaatc cacataaaat tacaaatgaa cgtattaaaa aagaaatgat
     2581 tgatgactta aattcaatta ttgatgattt ctttatggaa actaaacaaa atagaccgaa
     2641 atctataacg aaatataatc ctacaacaca taactataaa acaaatagtg ataataaacc
     2701 taattttgat aaattagttg aagaaacgaa aaaagcagtt aaagaagcag atgattcttg
     2761 gaaaaagaaa actgtcaaaa aatacggaga aactgaaaca aaatcgccag tagtaaaaga
     2821 agagaagaaa gttgaagaac ctcaagcacc taaagttgat aaccaacaag aggttaaaac
     2881 tacggctggt aaagctgaag aaacaacaca accagttgca caaccattag ttaaaattcc
     2941 acagggcaca attacaggtg aaattgtaaa aggtccggaa tatccaacga tggaaaataa
     3001 aacggtacaa ggtgaaatcg ttcaaggtcc cgattttcta acaatggaac aaagcggccc
     3061 atcattaagc aataattata caaacccacc gttaacgaac cctattttag aaggtcttga
     3121 aggtagctca tctaaacttg aaataaaacc acaaggtact gaatcaacgt taaaaggtac
     3181 tcaaggagaa tcaagtgata ttgaagttaa acctcaagca actgaaacaa cagaagcttc
     3241 tcaatatggt ccgagaccgc aatttaacaa aacacctaaa tatgttaaat atagagatgc
     3301 tggtacaggt atccgtgaat acaacgatgg aacatttgga tatgaagcga gaccaagatt
     3361 caataagcca tcagaaacaa atgcatataa cgtaacaaca catgcaaatg gtcaagtatc
     3421 atacggagct cgtccgacat acaagaagcc aagcgaaacg aatgcataca atgtaacaac
     3481 acatgcagat ggtactgcga catatgggcc tagagtaaca aaataagttt gtaactctat
     3541 ccaaagacat acagtcaata caaaacatta cgtatcttta caacagtaat catgcattct
     3601 atgatgcttc taactgaatt aaagcatcga acaatcggaa gcatatttct aaattattta
     3661 ttcattatag tcttaaacat aacatgacct aatatattac taacctatta aaataaacca
     3721 cgcacatcta agtgatatac gacaatcaca gcaataataa ttgctttaga aagtcgtgcc
     3781 gaactggaac ttacaagtct agttcgaaca cacactgatg tgagtggttt tctttatttt
     3841 aaacatgaac aatcagataa gttactagca ttagcaaata ttattaaatc aaagggcttc
     3901 gattcataaa atttaaaaca atgattaaaa ttagacgtgt aaatgttaaa ttctaaaacg
     3961 gaaataacca ccatcccatt aaaccacttt tttgttcaat cactatattt cacacagctt
     4021 cattaataaa acgaaattgc ttcaacccgc ttcaacttca actggcttca acttcagcct
     4081 acttcattca ataacaaaac gaatccgctt catccaaaat caaccattct aacgcacata
     4141 ttcaaatata gcagctgcac ccatgccgac accaatacac atcgtaacca tgccgtaacg
     4201 gctatcggga cgtctaccca tttcattaag taaacgcgcg gttaacattg cgcctgtagc
     4261 acctaatgga tgacctaaag caatagcgcc accattcaca ttcgtacgtg atatatctag
     4321 acctacttct ttaatagatg caatcgtttg agaagcaaat gcttcgttca attcgatcaa
     4381 atcaatgtct tcaacagata gattgctgag tgacaatact tcaggaatcg catatgcagg
     4441 cccaataccc ataattttcg ggtcaacgcc tactgcctta aaaccaacga atcgtgcaat
     4501 aggtgtcacg ccgagttctt tcactttatc tccagacatt aaaactacaa atcctgcacc
     4561 atcagaaagt ggggcagatg ttcctgcagt cacagtgccg tcagctttaa atactgtacg
     4621 taatttggct aatgcctcca tcgtggtgtc agggcgtata aattcatctt ggtcaaagat
     4681 atttgtgtgt acttttggtc ctgcgtttgt atattcaact gagtttactt gtattggaat
     4741 aatttcatct ttgaaccgac catcacgttg tgcgtcatag gcacgttgat gacttctgac
     4801 agcataagca tcttgatctt cgcgtgatac gtcaaattgg gatgctacat tttcagcagt
     4861 taaacccata ggatatgacg cacctatatc atcatattgt aaggttggat tgtttgtggg
     4921 ctcgttgcca cccattggta cggcactcat caattcaacg ccaccagcta caagtatatc
     4981 tccttgacca ggcataattt gattggctgc aatcgcgatg gtttgtaatc ctgatgagca
     5041 gtagcgattc actgtttgac ccggtaccgt gtcagataat cccgcacgca atgcaatcgt
     5101 tcgtgcaatg ttttggcctt gtaatccttc tggaaaagcc gtaccaacaa tgacatcttc
     5161 aatcatattc ttattgaatt ttccgtcaat acgtttcaat acgccttgta atactttggc
     5221 tgcgacatca tcaggtcttt cgtggaataa tgcgccttgc tttgctttcg ctgcggctga
     5281 acgcccataa gctacaatgt atgcttcttg catggttatc atcctctctt aatgactatc
     5341 ttttaattac gtaatggctt accagttttt aacatatgtg caattctttc atatgatttt
     5401 ttagatttta gtaagtcaat aaagccaatt ttctccaacg attgaatgta acgttgattg
     5461 ataaatgtat ttcttggtaa atcaccaccc gctaaaattg tggcgatatt taaggcaata
     5521 tgataatcat ggtcgctaat aaaatgaccc cgtctttgcg catctaattg tccttggatc
     5581 aatgctttga agtcttcacc taaagcgata tattgatgtc taggattcgg aatatagttt
     5641 gtttctgctt catatttcgc acgtttgagc gcaacttcga cacgttgtgc tgtattgaaa
     5701 ataatcgtat ctgtatcacg taaataacca taacgacgtg cctcaaaggc atttgtagag
     5761 actttcgcaa atgcgatatt cgtcagtact tttgtcatgg aagcttgttt gtcatcaaac
     5821 ttatgcgatg tgcgtaatat gcgatcagcc atttctgcaa ggccaccgcc actcggtaat
     5881 aagccaacac ctgcttcaac aagaccgata tatgtttcac ttgcagcgac aacaataggt
     5941 gagtaaagta caagctcaca gccaccgcct aaggcacgac cttgaacagc tgtgactact
     6001 ggtttcaaac tatacttcaa acgattaaag ctataatgta atttatcaat tgattgtgca
     6061 acgacatcat ctacaagacc gtcttcatgc gcctttttca ttaagaaaag gttagcaccc
     6121 acactgaaat tgttaccatc tgcataaata accatacttg tgtaatggtc attttccagt
     6181 aaatcaatcg catcaactaa cgcatcgttg aattcatcgg taatgacatt atttttactt
     6241 tgtaatttca gtaacagttg atcatcatga gttacggaaa gtttggcatc acctttatcc
     6301 caaagttcat cttttacgaa gtgagaaata ggtgttgcat attcaatggt ctcatcttgt
     6361 ttataaaagc caccatctaa atcactaatc cattgtggta agtctccaag ttcgtcttcc
     6421 atacgtgttt taacacgttc gtatcccatt gcatcccata attggaatgg accaagtttc
     6481 cagttgaacc cccagacaag cgcacggtct atgtctcgga aatcatcggt agctttaggt
     6541 acattgatag cagagtaata gaaattatta cgtaatgtct cccataaaaa tagtcccgct
     6601 tcgtcttgcg cattgaatat ggtatcaagg ttatgcacta agtctttatt aaattcattt
     6661 aaaattggta attgtggttg cgatacaggt acataatctt gtttttcaac atcgtaaaca
     6721 agtcgagctt tagtttcttt atcctttttg taaaatcctt gtttcgtttt acgtccgagt
     6781 gcgccattgt caaacaacgt atttacaatt ttgacatcat gaaaataagg tgtttcttca
     6841 ggtacttgtt gcatgccttt aattacagac actgcaatat ctaaaccgac taggtcagat
     6901 agcgcatatg tacctgtttt aggacgacca atcgcttgcc cagttaaagc atccacatct
     6961 acaatgctta tcttgtgttg ctcggcgcga tacataatat cattcattgt ttgcgtgccg
     7021 actctatttg cgacaaagcc aggcacatca ttgacgacaa tgacaccttt acctaacaca
     7081 ttttgcgcga aattttttac atctaatata atagattcct tcgtgtgtga cgtaggtatt
     7141 aactccacta atttcataat acgtggtggg ttaaagaaat gtagaccaaa gaatcgttct
     7201 tgatccttct cgttaaatgc ttgagcaatc gcattaattg gaatacctga tgtatttgta
     7261 gcgaataaag catcttcttt agcatgttgt agaacttgtt gccaaacagc atgcttaatt
     7321 tcaatatctt ctttgactgc ttcgatatat aaatcagcat catcatttac caagtcatca
     7381 tcaaaattac catatgttaa atgactcgct agatttaagt cgaatagtag cggccgtttc
     7441 ttatctgtaa ttttatcgta agattttttc gcaatgagat ttggatcgtt tttgtccact
     7501 acaatatcta atagttttac tttaagtcca gcattcacaa aaagtgctgc cagttgagcg
     7561 cccattgtgc ctgcgccaag aacggttact ttattaattg tcatagtgat tcctccaatt
     7621 tagttgagga taagataacc attaagataa ttggaataac gttgctattt tataaaatta
     7681 attaagtatc tttgacagtc atcttagcct cttatttaag gaaaaagctt tatgcttaaa
     7741 ataagtcttt tttagtgaaa ttaatgcatc tcatataatt atttgctatt tatacgaaag
     7801 cagaatctcc agtcaaagcg cgtccaatta ctaaggcatt aatttcatgt gtaccttcgt
     7861 acgtgtaaat cgcttctgca tcagagaaga aacgtgcaat atcataatcg tcagctagta
     7921 tgccattacc acctgtaata ccgcggccca tagctactgt ctcacgcaaa cgtaaggcat
     7981 tcatcatctt cgccgttgaa gttgcaacct cgtcatattc accatgtgct tgcatattag
     8041 ctaattgagc acatgttgcc attgcttgag ctaaattacc ttgcatcatt gctagctttt
     8101 cttgtattaa ctgatattta ctaattggtt tgccgaattg cttacgctca gtgacataat
     8161 ctaatgtggc acgtaaagcg ccagccatac cacctgtagc catataagca acgcctgctc
     8221 tcgttgaata aagaattttg gcaatatctt taaagcttgt tatgttttgt aagcgatccg
     8281 cttcatctac tttgacatta gttaatttaa ttagggcgtt aggaacaatg cgaagtgcga
     8341 ttttattatc aatgacttca atatcgacgc catcttgttc tggtctgact acaaagcaat
     8401 ggggtttgcc agtttcttta tttactgcga atactggaat gacatcagat acatgtgcac
     8461 caccaatcca tttcttttca ccattgataa cccaagtatc gccttggcgt tcagcgactg
     8521 tttcaagacc tcccgcaacg tccgaaccgt gttctggttc agttaaagca aagcatgtac
     8581 gcagttcatg tgactgtaat ttaggtacat atttcgcaat ttgttctttg ctacctccga
     8641 aatagaaagt gttatgccct aaaccttggt gaacaccgag tagggtagct aaggaaatat
     8701 caaatcgcgc gagtaggtaa gacatgaaaa actgaaatag ttgactaggc attttggcgt
     8761 ttggacgatc cttgtaaagt aatggattgt taaaataatt taattctccc agatctttaa
     8821 aatagtcctc gggtacagta gcgtctatcc aatgttgatt aatattttca cggtacttac
     8881 tttctagcaa tgaatctact tgttgtaaaa attcgacttc accgtctgtt aaacctttag
     8941 caatactaag tacatcttca ggaaataatg tttttaagac cgtttctttt tcaaatgtca
     9001 tataaattcc tcctaaaaat aatatgaata ctaatgtgaa atgcatttaa ttcaaaaaca
     9061 acacgcttta tttgtaaacg cttacactaa atgtcaaaaa tttttatcac ctttaaagtg
     9121 tttgcgagac tttgtcattc atcatttgtc gaatcgcaag tttatctggt ttctgcgtac
     9181 tgtttaacgg catatgtgtc actggtacat acattcttgg gactttataa cctgctaaac
     9241 gacttcgcat atgttgattt aaaatttcag cgtaatgagg ttcatcttcg cgaagtataa
     9301 tggctgcagc aattgattca ccatattttg gatgatcata gccaacgacc acacaccggt
     9361 ctactagtgg atgctcagct aaagcatttt cgacttcgga tggtaagaca ttttcgccac
     9421 cagttatgat taattctttt ttgcggtcaa taataaatat atcgccatcg ttgtccatct
     9481 tcgctaagtc accagttaat aaatatcgac catgaaatgc tttggcagtc tctgctggtt
     9541 tattccaata tcctggcgtg acatttttag ccttaattgc aagttcgcca atctcaccag
     9601 taggtacttc ctcaccgtta tcatcaagga tacgtgcatc aacgaacatg actgctttac
     9661 caatactcat tggcttacgt tttgaatttt ccggtgtatt aacaagtaca agaggtgctt
     9721 cagttaaacc atagccgtta ataatgttta tgccatattg tttaaaagct gcttggatac
     9781 ttggtaatgg ttgtgaacca ccttggatga tataatccat agctctaaaa ttttcaggat
     9841 taaaattact agcacgtagc gtactataat acattgtcgg aatcatgata ataaatgtag
     9901 ggtgatattg tgcaatcatg tcattcaatt cttcgccgtt aaagtaacgt tgaagaataa
     9961 gtgtgccacc tgacattaat actggtaata cagtatcgtt aaaccctaaa acatggaaca
    10021 ttggtgttga tacaatcgta atatagtttg aattgaactt atacgtcagc tctaagtttg
    10081 caccgttatg aacaaatgat tcatatgaga acatcacacc tttaggtgat ccggttgtac
    10141 cacttgtata aattaatgct gcaagatctt gtggttcaac aggtgttgct tgaaaaggtt
    10201 ggtgataatc tggatttacg atttcatcat attgcgccac atcaatatcc atatgcaata
    10261 agttttggtc aatatcggtg agtgaactta aatgtttttc agcatagaag agcagtttta
    10321 attgtgcatc ttccacaatg gctgcaattt cttttgggtt aagccgccaa ttcaatggta
    10381 aaaaaaccgc acctgtttta aaacaagcaa acaataaatc taatattgca atatcatttg
    10441 gcgcaaaaat accgataaca tcgccttttt taacaccttg agatgttaaa taatgtgcca
    10501 tattatcagc gcgtgcattg agttgttggt atgtccaaga tgtttgtttt gcgtgatcaa
    10561 taacggcagg cttgtcatca tcgaagtctg aacgcgtttt tatccaatcg aaattcatta
    10621 gtataccccc tttagcttca ctttcatact ttatgaattg attgtttaag ttgtccccat
    10681 ttttctttgt aaatgctggt atcaattaat tttaaatgat cagcaataat tggtttaaaa
    10741 gccatttgat tcaaaatatc tttatgcaaa tcaagacctg gtgcaatttc aattagtttc
    10801 aagccttgat tggtgagttc gaatactgca cgatcagtaa caaaatagat ttcttgctcg
    10861 agtgattgtg aatattgtgc attaaagtcg atatggctca catctgatac aaatttctgg
    10921 ttttgtcctt cagtttcaat gtttaatcgt tgattatggc atgagacatg actgccagct
    10981 acaaaagtac ctgaaaagat aattttattt acagattgcg taatgtctat aaagccacca
    11041 catccattta gtcggtcatt gaagtaagac acgttgacat tgccgtattg atcaacctca
    11101 gcaaagctaa gataggcaac tgatacacca ttgttataaa taaaatccca tgctcgatca
    11161 tgaggcatgc gcacatctgc attgtaattc ataccaaaat gttcacgact cccaacgaat
    11221 ccaccgaaaa tgccaacatc taaaatcggt tgcacatcat gttcaacaca ttcttcatgc
    11281 aataaattag agagttcatt attgatgcca taaccgatgc taattgtatc gccataagtt
    11341 aaaaactgag cagcacgtcg gagaatcaat ttgcgactat taaaaggtaa tgcgggttca
    11401 ggtattccat caattcgttc ttctccagac aaggctggta aataatgact ttgaattact
    11461 tggcggtgat tcttttcatc ttctgtgacg tatacataat cgacaagatt tcctggtata
    11521 acaacttcat tcggttttag ttgatagtcg tcaactaaag ctttaacttg tacaataact
    11581 ttcccatgat tggctttcgc gtttaatgcg acatgataac actcgctcaa gtacgcttct
    11641 tgagttaaat aaatgttacc ttgttgatct gcgtatgttc ctctcagtag tgccacatca
    11701 acgctaggga atgtgtaatg taagtatgtt tcatcgttga tggttactaa tgaaactaaa
    11761 tcatccgttg ttcgtgtatt tactttaccg ccaccgtatc taggatcaac agctgtgttt
    11821 aatccgattt tagtaataac tccaggtaat aattgattac tctgacgata atgagttgca
    11881 atgatacctt gtggtaaaaa ataagcttca atgtcattat ttttcattgc ttgtgccgtt
    11941 ttggaagaag ccgttaaaat actcataatg acacgtttaa tcatgcgacg ttctataaaa
    12001 tcatctaaat ccggtgcggc acctaaacta tgaatatcat tcgctaatat aaacgttaaa
    12061 tcattgggcg tatgatatgt gtcatgttgc gctaacacag cacgtagaac ttcggcgggt
    12121 aagttggcta cagctaatgc tggtaaacca atcacatcac catctttaat gatatgttgt
    12181 aagtcgtgcc atgtgatttg tttcaagcaa gtcacctcca tcacatttga taaaatatag
    12241 cgtttttaca ctttgtgtaa acccttacaa gaaatataac ataacgacgt ttaaaatcaa
    12301 ttagaaatat ctttttattc tgataataga cacagtatag acacattttg atggtcgata
    12361 acaattgtaa tatcaagggt ttgtaatgaa ttgaatatca ttaaaatact tatataaaaa
    12421 tattgttcgg aatataaaaa gttaaatagg ttttgatttt taaata
//
LOCUS       DAXWFK010000074        12428 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.074, whole genome shotgun sequence.
ACCESSION   DAXWFK010000074 DAXWFK010000000
VERSION     DAXWFK010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12428
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.074"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            172..1185
                     /locus_tag="ACGEXS_001528"
     CDS             172..1185
                     /locus_tag="ACGEXS_001528"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFF8012540.1"
                     /translation="MAIKYSALNLVPIREGEDEQTAINDMVNLAQHLDELSYERYWIA
                     EHHNAPNLVSSATALLIQHTLEHTKHIRVGSGGIMLPNHAPLIVAEQFGTMATLFPNR
                     VDLGLGRAPGTDMMTASALRRDQHDGVYKFPEEVSLLQQYFGPAHQQAYVRAYPAVGK
                     NVPLYILGSSTDSAHLAARKGLPYVFAGHFAPQQMKEAIEIYKTLFEPSDVLDEPYVI
                     VCLNTIVAENDDEAQYLASSMAQVMVSITRGRMQPVQPPTHELQNILTPREYAMAMER
                     QKISLIGSENTVQQKIQDFMETYGEVNEIMAISYIYDKDMQLDSYRRFKNVINQINEK
                     NTL"
     gene            complement(1422..1568)
                     /locus_tag="ACGEXS_001529"
     CDS             complement(1422..1568)
                     /locus_tag="ACGEXS_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAS049 family protein"
                     /protein_id="HFF8012541.1"
                     /translation="MSFMDKAKDAVEKFKNSDNEQVKNVKDKINEYTGSNNEEKKENE
                     DKEK"
     gene            complement(1608..1790)
                     /locus_tag="ACGEXS_001530"
     CDS             complement(1608..1790)
                     /locus_tag="ACGEXS_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFF8012542.1"
                     /translation="MADESKFDQFKGNVKETVGNVTDNKELEKEGQQDKATGKAKEVV
                     ENAKNKITDAIDKLKK"
     gene            complement(1890..2312)
                     /locus_tag="ACGEXS_001531"
     CDS             complement(1890..2312)
                     /locus_tag="ACGEXS_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002458938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFF8012543.1"
                     /translation="MKISTKGRYGLTLMISLAKKEGQGCISLKSIAEENNLSDLYLEQ
                     LVGPLRNAGLIRSVRGAKGGYQLRVPAEEISAGDIIRLLEGPITFVESIESEPPAQKQ
                     LWIRMRDAVRDVLDNTTLKYLAEYVDTSEDLDGYMFYI"
     gene            2397..3671
                     /locus_tag="ACGEXS_001532"
     CDS             2397..3671
                     /locus_tag="ACGEXS_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFF8012544.1"
                     /translation="MSTEPLASRMRPKNIDEIISQQHLVGPRGIIRRMVDTKKLTSMI
                     FYGPPGIGKTSIAKAISGSTQYKFRQLNAVTNTKKDMQLVVEEAKMSGQVILLLDEIH
                     RLDKAKQDFLLPHLENGKIVLIGATTSNPYHAINPAIRSRAQIFELYPLNDEDVRQAL
                     TRAIEDDENGLKTYQPKIDEDAMTYFSTQSQGDVRSALNALELAVLSADNDKDGYRHV
                     TLQDAKDCLQKGAFVSDKDGDMHYDVMSAFQKSIRGSDVNAALHYLARLIEAGDLPTI
                     VRRLLVISYEDIGLASPNAGQRTLAAIESAERLGLPEARIPLSQAVIELCLSPKSNSA
                     MSAIDSALSDIRNGHVGQIPNHLKDGHYQGAKDLGRSIGYKYPHQYVNGYVSQQYLPD
                     KLKNKIYYEPKTTSKSEQQLKEIYNNLLKQRP"
     gene            complement(3832..4605)
                     /locus_tag="ACGEXS_001533"
     CDS             complement(3832..4605)
                     /locus_tag="ACGEXS_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HFF8012545.1"
                     /translation="MKHQFSRNELAIGQQGLDLLKQQTVVILGVGGVGSFAAEALART
                     NIGHIILIDKDDVDITNVNRQLHALTTTVGQSKVSLMEERIKLINPDCKVTPLHMFYT
                     EETYEEIFDNYDIDYFVDASDTIMYKVHLMKECLDRGIKVISSMGAANKTDPTRFTIS
                     DISKTHTDPMAKIIRQKLKKLGIRKGIPVVFSDESPIVIREDVKDIVGDKNAINRKGQ
                     MPPSSNAFVPSVVGLICASYVVNDVLKDIPVRRIKDKGQ"
     gene            complement(4605..4733)
                     /locus_tag="ACGEXS_001534"
     CDS             complement(4605..4733)
                     /locus_tag="ACGEXS_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012546.1"
                     /translation="MGFIAFNKCFLVEFWENNQSDNAKYKILSYYVLMYKSVKEVK"
     gene            complement(4798..4988)
                     /gene="ssrS"
                     /locus_tag="ACGEXS_001535"
     ncRNA           complement(4798..4988)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACGEXS_001535"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(5066..6832)
                     /gene="aspS"
                     /locus_tag="ACGEXS_001536"
     CDS             complement(5066..6832)
                     /gene="aspS"
                     /locus_tag="ACGEXS_001536"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFF8012547.1"
                     /translation="MSKRTTYCGLVTEAFLGQEITLKGWVNNRRDLGGLIFVDLRDRE
                     GIVQVVFNPAFSEEALKIAETVRSEYVVEVQGTVTKRDPETVNPKIKTGQVEVQVTNI
                     KVINKSETPPFSINEENVNVDENIRLKYRYLDLRRQELAQTFKMRHQITRSIRQYLDD
                     EGFFDIETPVLTKSTPEGARDYLVPSRVHDGEFYALPQSPQLFKQLLMISGFDKYYQI
                     VKCFRDEDLRADRQPEFTQVDIEMSFVDQEDVMQMGEEMLKKVVKEVKGVEINGAFPR
                     MTYKEAMRRYGSDKPDTRFEMELIDVSQLGRDMDFKVFKDTVENDGEIKAIVAKGAAE
                     QYTRKDMDALTEFVNIYGAKGLAWVKVVEDGLTGPIGRFFETENVETLLTLTGAEAGD
                     LVMFVADKPNVVAQSLGALRVKLAKELGLIDETKLNFLWVTDWPLLEYDEDAKRYVAA
                     HHPFTSPKEADIAKLGTAPEEAEANAYDIVLNGYELGGGSIRIHDGELQEKMFEVLGF
                     TKEQAQEQFGFLLDAFKYGAPPHGGIALGLDRLVMLLTNRTNLRDTIAFPKTASATCL
                     LTNAPGEVSDKQLEELSLRIRH"
     gene            complement(6848..8110)
                     /gene="hisS"
                     /locus_tag="ACGEXS_001537"
     CDS             complement(6848..8110)
                     /gene="hisS"
                     /locus_tag="ACGEXS_001537"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFF8012548.1"
                     /translation="MIKIPRGTQDILPEDSKKWRYIENQLDELMTFYNYKEIRTPIFE
                     STDLFARGVGDSTDVVQKEMYTFKDKGDRSITLRPEGTAAVVRSYIEHKMQGNPNQPI
                     KLYYNGPMFRYERKQKGRYRQFNQFGVEAIGAENPSVDAEVLAMVMHIYQSFGLKHLK
                     LVINSVGDMASRKEYNEALVKHFEPVIHEFCSDCQSRLHTNPMRILDCKVDRDKEAIK
                     TAPRITDFLNEESKAYYEQVKAYLDDLGIPYIEDPNLVRGLDYYTHTAFELMMDNPNY
                     DGAITTLCGGGRYNGLLELLDGPSETGIGFALSIERLLLALEEEGIELDIEENLDLFI
                     VTMGDQADRYAVKLLNHLRHNGIKADKDYLQRKIKGQMKQADRLGAKFTIVIGDQELE
                     NNKIDVKNMTTGESETIELDALVEYFKK"
     gene            complement(8571..9446)
                     /locus_tag="ACGEXS_001538"
     CDS             complement(8571..9446)
                     /locus_tag="ACGEXS_001538"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFF8012549.1"
                     /translation="MKKIEAWLSKKGLKNKRTLIVVIAFVLFIIFLFLLLNSNSEDSG
                     NITITENAELRTGPNAAYPVIYKVEKGDHFKKIGKVGKWIEVEDTSSNEKGWIAGWHT
                     NLDIVADNTKEKNPLQGKTIVLDPGHGGSDQGASSNTKYKSLEKDYTLKTAKELQRTL
                     EKEGATVKMTRTDDTYVSLENRDIKGDAYLSIHNDALESSNANGMTVYWYHDNQRALA
                     DTLDATIQKKGLLSNRGSRQENYQVLRQTKVPAVLLELGYISNPTDETMIKDQLHRQI
                     LEQAIVDGLKIYFSA"
     gene            complement(9443..9895)
                     /gene="dtd"
                     /locus_tag="ACGEXS_001539"
     CDS             complement(9443..9895)
                     /gene="dtd"
                     /locus_tag="ACGEXS_001539"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFF8012550.1"
                     /translation="MKVVVQRVKEASVTNDTLNNQIKKGYCLLVGIGQNSTEQDADVI
                     AKKIANARLFEDDNNKLNFNIQQMNGEILSVSQFTLYADVKKGNRPGFSNSKNPDQAV
                     KIYEYFNDALRAYGLTVKTGEFGTHMNVSINNDGPVTIIYESQDGKIQ"
     gene            complement(9907..12096)
                     /locus_tag="ACGEXS_001540"
     CDS             complement(9907..12096)
                     /locus_tag="ACGEXS_001540"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFF8012551.1"
                     /translation="MNNEYPYSADEVLHKAKSYLSADEYEYVLKSYHIAYEAHKGQFR
                     KNGLPYIMHPIQVAGILTEMRLDGPTIVAGFLHDVIEDTPYTFEDVKEMFNEEVARIV
                     DGVTKLKKVKYRSKEEQQAENHRKLFIAIAKDVRVILVKLADRLHNMRTLKAMPREKQ
                     IRISRETLEIYAPLAHRLGINTIKWELEDTALRYIDNVQYFRIVNLMKKKRSEREAYI
                     ETAIDRIRTEMDRMNIEGDINGRPKHIYSIYRKMMKQKKQFDQIFDLLAIRVIVNSIN
                     DCYAILGLVHTLWKPMPGRFKDYIAMPKQNLYQSLHTTVVGPNGDPLEIQIRTFDMHE
                     IAEHGVAAHWAYKEGKKVSEKDQTYQNKLNWLKELAEADHTSSDAQEFMETLKYDLQS
                     DKVYAFTPASDVIELPYGAVPIDFAYAIHSEVGNKMIGAKVNGKIVPIDYILQTGDIV
                     EIRTSKHSYGPSRDWLKIVKSSSAKGKIKSFFKKQDRSSNIEKGRMMVEAEIKEQGFR
                     VEDILTEKNIQVVNEKYNFANEDDLFAAVGFGGVTSLQIVNKLTERQRILDKQRALNE
                     AQEVTKSLPIKDNIITDSGVYVEGLENVLIKLSKCCNPIPGDDIVGYITKGHGIKVHR
                     TDCPNIKNETERLINVEWVKSKDATQKYQVDLEVTAYDRNGLLNEVLQAVSSTAGNLI
                     KVSGRSDIDKNAIINISVMVKNVNDVYRVVEKIKQLGDVYTVTRVWN"
BASE COUNT         3857 a         2390 c         1766 g         4415 t
ORIGIN      
        1 tttaagttta tcgctttatt cttgtctttt ttataaattt tagtatttgc agatattttt
       61 ttatttgtaa aatgtaacgt actattattt tggttatgag caatttaata tttatctggt
      121 tattcgattg gtatacttct tatatcataa aaaaggaagg acgatataaa aatggcgatt
      181 aaatattcag cattaaacct tgtccctatt cgagaaggtg aagatgaaca aacagcaatt
      241 aatgatatgg ttaatctcgc acaacattta gacgaattat catatgaaag atattggatt
      301 gctgaacacc ataacgctcc caacctagta agttcagcaa ctgctttatt aattcaacat
      361 acgttagaac atacgaaaca catacgtgta ggttctggag gcatcatgtt acctaatcat
      421 gctccattaa tcgttgcgga acaatttggc acgatggcaa cattatttcc aaatcgtgtc
      481 gatttaggat taggacgtgc acctggaaca gatatgatga ccgcaagtgc attaagacga
      541 gatcaacatg atggtgttta taaatttcca gaagaggttt cattattaca acaatatttc
      601 ggccctgctc accaacaagc atatgttcgt gcttatccag cagtaggtaa aaatgtgcct
      661 ttatacattc ttggttcttc aacagattct gcacatttag ctgctcgcaa agggcttcca
      721 tatgtgttcg ctggacattt tgcacctcaa caaatgaaag aagctatcga aatttacaaa
      781 acgttatttg aaccttctga tgtattagac gaaccttatg ttattgtatg tttaaataca
      841 atcgttgctg aaaatgatga cgaagcacaa tatttagctt catctatggc acaagtaatg
      901 gttagtatca ctcgtggcag aatgcagccc gttcaaccgc caacacatga actacaaaat
      961 atattaacgc cgagagaata cgcgatggct atggaaagac agaaaatatc attaataggt
     1021 tcagaaaata ctgttcaaca aaaaattcaa gattttatgg aaacttatgg tgaagtcaac
     1081 gaaattatgg caataagtta tatttatgat aaagatatgc aattagactc ttatcgtcgg
     1141 ttcaagaatg ttataaatca gataaatgaa aaaaacactt tataatgtga taaataaact
     1201 aagtgaaagt atgtatccat aatattaata aaaatataca gtaacagcat tttgaatgaa
     1261 agatgtcttt attgttcaat catttatttt agtaatgatt caaattcact taaaattcta
     1321 atgcaaatat gaaagcgccc cttcaacttt acactgtgta agtgtttatt tgatggggcg
     1381 ctttcaaaat attgaaaagc atatccaaaa tttaaagaaa tttatttctc tttatcttca
     1441 ttttcttttt tctcttcgtt attcgatcct gtatattcat ttatcttatc ttttacattt
     1501 ttaacttgtt cattatcgct atttttaaat ttttctactg cgtctttagc tttatccata
     1561 aaactcatat taatcgctcc tcttatattt gattagttta attgaactta ttttttaagt
     1621 ttatcaattg catcagttat tttgttttta gcattttcaa caacttcttt tgctttacca
     1681 gtcgctttat cttgctgacc ttctttttct aattctttgt tatcagtaac gttacctact
     1741 gtttctttaa catttccttt aaattgatcg aacttacttt cgtctgccat agtgaaacct
     1801 ccttggatgt atatatttat ataccactaa ggaggttcgc taaacattat aatatgaagt
     1861 ttttatgtta tagtatagta tttatacgat taaatataaa acatgtatcc gtctaaatct
     1921 tcacttgtat ctacatattc cgctaaatat ttcaatgttg tattatctaa aacatctctc
     1981 actgcatctc tcatgcgaat ccatagttgt ttttgcgcag gtggttctga ttcaatactt
     2041 tcaacaaatg taattggacc ttctaacagt cttataatat cccctgctga gatttcttcc
     2101 gctggcactc ttaattggta tccaccttta gcaccgcgta cacttcgaat taaccccgca
     2161 tttcttaaag gacctacaag ctgttctaaa tataaatcac tcaaattatt ttcttcagca
     2221 attgacttta atgatataca tccttgcccc tcttttttag caagagaaat catcaatgta
     2281 agtccatatc tccctttagt agaaattttc attgtataac ctcacttaat tcgaatattg
     2341 atattcccat tttagcattt tttgagttaa gatagtataa gaaaggtgtg acaaatgtga
     2401 gtacagaacc attagcatcg agaatgcgcc caaaaaatat agatgaaatc atttcccaac
     2461 aacatttagt tggaccaaga ggcattatca gaagaatggt tgatacaaaa aaattaactt
     2521 caatgatttt ttatggtcca cctggtatag gcaaaacaag tattgccaaa gcaatttcgg
     2581 gcagtacgca atataaattc agacaattga atgctgtaac taacactaaa aaagatatgc
     2641 aacttgttgt tgaagaagct aaaatgtctg gtcaagttat cttgttatta gatgaaatac
     2701 atcgactaga taaagctaaa caagactttt tattacctca tttagaaaat ggcaaaatcg
     2761 tcttgatcgg tgctacaact tcaaatcctt atcatgctat caatccagcg attcgttcaa
     2821 gagcgcaaat tttcgagtta tatcctttaa atgacgaaga tgtgcgccaa gcgttaactc
     2881 gtgcaataga agatgatgag aatggtttga aaacatatca acccaaaatt gatgaagatg
     2941 ccatgaccta cttttctaca caaagccaag gtgatgttcg tagtgcgtta aatgcattgg
     3001 aattagctgt attaagcgca gataatgaca aagacggtta tcgacatgtt acattgcaag
     3061 atgctaaaga ctgtttacaa aaaggtgcat ttgtaagtga caaggatggt gacatgcatt
     3121 acgatgttat gagcgctttc caaaaatcta tccgtggtag cgacgtcaat gccgctttac
     3181 attatttagc acgattaatt gaagctggag atttacctac aatagttcga cgattacttg
     3241 taattagcta tgaggatata ggcttagcct cacctaatgc tggtcagaga acacttgctg
     3301 ctattgaatc agcagaacgt ctaggtttac cagaagctag aattccacta agccaagcag
     3361 taatcgaact atgcttatca cctaagtcaa attcagcaat gagtgccatt gatagtgcat
     3421 tgtccgatat tagaaacggt catgtgggcc aaattccaaa ccatttaaaa gatggacatt
     3481 atcaaggtgc taaagatcta ggccgatcta ttggttacaa atatccacac caatatgtta
     3541 atggctatgt ttcacagcaa tatttacctg ataaactaaa aaacaaaatt tattacgaac
     3601 caaaaacgac atctaaaagt gaacaacaac tcaaagaaat atataacaac ttacttaaac
     3661 aaaggccgta aaaataagat taaatcgatt acctttgata ttttagctat aatcaaatca
     3721 aaactgacta gtacacgagg catattctac tatgcttgcg aactagtcag ttttagtttt
     3781 atggtgtggt tacacttgcc ttacgttgaa aattatccct ttcaaaatga attattgccc
     3841 tttgtcttta atgcgacgaa ctggaatatc ttttaatacg tcattcacca cataacttgc
     3901 acaaattaat ccaacaacac ttggcacaaa ggcatttgaa gaaggtggca tttgtccttt
     3961 tcgattgata gcatttttat ctccaactat atcttttaca tcttctctta tgacaattgg
     4021 actttcatct gaaaatacaa ctggaatccc tttacgaatt cctagttttt tcaatttttg
     4081 acgaataatt ttggccattg gatcggtatg tgttttagag atatctgaaa ttgtaaaacg
     4141 tgttggatct gttttatttg cagcacccat actggaaatc actttaatcc ctcggtcaag
     4201 acactctttc attaagtgta ctttgtacat tattgtatca cttgcatcta caaaataatc
     4261 tatatcgtag ttatcgaaaa tttcttcata tgtctcttct gtataaaaca tatgtaaggg
     4321 cgtgacttta caatctggat taattaattt aatacgttct tccatcaaag aaactttact
     4381 ttgtcctacc gttgtagtta aagcgtgtaa ttgtctgttt acatttgtaa tatcaacatc
     4441 atctttatct attaatataa tatgaccaat attcgttctt gctaatgctt cagcagcaaa
     4501 tgaaccaaca cctccaacgc caagtatgac aacagtttgt tgcttcaata aatctaaacc
     4561 ttgttgtcca atcgctagtt catttcttga aaattgatgt ttcattattt tacctctttc
     4621 actgatttat acataagtac atagtaactt aaaattttat atttagcatt atcactttga
     4681 ttattttccc aaaattcaac gaggaaacat ttattaaacg ctataaaacc caactaattc
     4741 tttattaaaa acttaaagaa acgcataaaa atacgcaaga caaagtcttg cgtatcgata
     4801 gagtccgtat tgccgtagtt ataatagctt gatcattcgg cctgttatat acaggtgggt
     4861 gccctgtttc ttgttttgta cgtccttcat ataaggcgtg tacgctgcaa gaaaacccat
     4921 tgggctccct tgatcaaaga gtgttaggcc caaattaaaa agcaaactta cgaacaactc
     4981 agatgactat cttatgatgt tatattacca cataattaaa attaatgaaa ttataacaaa
     5041 ccaaagttta ttgatttttt aaaatttagt gacgaattcg caaagaaagt tcttctaatt
     5101 gtttatcaga aacttcacca ggcgcattcg ttaataaaca tgtagcagat gctgttttag
     5161 ggaatgcgat tgtatctctc aagtttgttc tattagtcaa taacatgact aatcggtcta
     5221 atcctaatgc aataccgcca tgtggtggtg caccatattt aaatgcatct agtaagaagc
     5281 cgaactgttc ttgtgcttgt tctttagtaa atccaagaac ttcgaacatt ttttcttgta
     5341 actcaccatc atgaattctg attgaaccgc cacctaattc ataaccattt aatactatgt
     5401 cataagcatt tgcctcagct tcttctggcg cagtgccaag cttagcaata tcagcttctt
     5461 ttggagatgt aaatggatga tgtgctgcaa cgtaacgctt cgcatcttca tcatattcta
     5521 ataatggcca atctgtcacc cataagaagt ttaattttgt ttcatcgatt aaacctaatt
     5581 ctttagctaa tttgacacgt aatgcaccta aactttgtgc aacgacattt ggtttgtctg
     5641 caacaaacat tactaagtca ccagcttcag caccagttaa tgtaagtaat gtttcaacat
     5701 tttctgtttc aaagaaacgt ccaattggac ctgtcaaacc atcttccaca actttaaccc
     5761 acgctaatcc tttagcacca tagatgttta caaattctgt taaagcatcc atatctttac
     5821 gagtatattg ttcagctgca cctttagcga caattgcttt aatttcacca tcattttcaa
     5881 cagtatcttt aaatacttta aagtccatat cacgtcctaa ttgagaaacg tcaattaatt
     5941 ccatttcaaa acgtgtatct ggtttatcag aaccatagcg acgcatcgct tctttatatg
     6001 tcatgcgtgg gaaagcgcca ttaatttcaa cgcctttaac ttctttaaca acttttttaa
     6061 gcatttcttc acccatttgc atcacatctt cttggtctac aaaactcatt tcaatatcga
     6121 cttgtgtaaa ttcaggttga cgatctgcac gtaaatcttc gtcacggaag cattttacga
     6181 tttggtagta tttgtcaaat ccactaatca tcaataattg cttaaataat tgtggtgatt
     6241 gtggtaatgc ataaaattca ccatcatgaa cacgagatgg tactaaatag tcacgtgcac
     6301 cctcaggtgt tgacttcgtt agtactggtg tttcgatgtc aaagaaccct tcatcatcca
     6361 aatattgacg aatagaacgt gtaatttgat gtctcatttt aaatgtttgc gctaactctt
     6421 gacgacgtaa atctaaataa cggtatttta atcgaatatt ttcatcaacg ttaacatttt
     6481 cttcatttat agaaaatggt ggtgtctcag atttattaat cactttaata tttgtaactt
     6541 gtacttcaac ttggccagtt ttaattttag gattaactgt ttcagggtca cgcttcgtaa
     6601 ctgtaccttg aacttctaca acatattcag aacgtactgt ttcagcaatt ttcaatgcct
     6661 cttctgaaaa tgcaggatta aacacgactt gtacaattcc ttctctatct cttaaatcaa
     6721 cgaaaatcaa tccaccaagg tcacgacgat tgttaaccca tccttttaat gtaatttctt
     6781 gtcctaaaaa tgcctcagta actaatccac aataagttgt tctcttactc atattttaac
     6841 gccctctcta cttcttaaaa tattcgacta atgcgtctaa ttcaattgtt tcagattcac
     6901 cagttgtcat atttttaaca tcgattttat tattttctaa ttcttgatca ccaataacga
     6961 ttgtaaactt ggcacctaaa cggtctgctt gtttcatttg tcctttaatt ttacgctgta
     7021 aatagtcttt atctgcttta ataccattat gtctcaaatg atttaatagc ttcacagcat
     7081 atcgatctgc ttgatcaccc attgtaacaa tgaataaatc taagttttct tcaatatcta
     7141 attcgatacc ttcttcttca agtgcaagca ataatcgttc tatacttagc gcaaaaccaa
     7201 tacctgtttc acttggacca tctagcaatt ctaataaacc attataacgg ccaccaccac
     7261 aaagcgttgt aatggcacca tcatagttag ggttatccat cattaattca aatgctgtat
     7321 gtgtataata atccaatcca cgaactaagt taggatcttc aatatatgga atacctaaat
     7381 catctaaata agcttttact tgttcataat atgccttaga ttcctcattt aagaaatcag
     7441 tgattctagg tgcagtctta atcgcttctt tatcacggtc tactttacaa tccaaaattc
     7501 gcatcggatt tgtatgcaaa cgtgattgac aatctgaaca aaattcatga attactggtt
     7561 caaagtgttt cactaacgct tcgttatatt cttttcgaga cgccatatcc cctacactat
     7621 taataacaag ctttaaatgt tttaatccaa atgattgata aatatgcata accatagcta
     7681 atacttctgc atctacgcta ggattttcag caccaatagc ttctacacca aattgattaa
     7741 attgacgata gcgtcctttt tgcttacgtt catatctaaa catcggtcca ttgtaataaa
     7801 gtttaattgg ttggtttgga ttaccttgca ttttatgttc aatatatgaa cgcacaactg
     7861 cagctgttcc ctcaggtctt aatgtaatac ttctatcgcc tttatcttta aatgtataca
     7921 tttctttttg tacgacatcg gttgaatcac caacacctct tgcaaaaaga tctgtacttt
     7981 caaaaattgg tgttcttatt tctttataat tataaaatgt cattaattca tctaattgat
     8041 tttcaatgta acgccatttc tttgaatctt caggtaaaat atcctgcgtc cctctaggta
     8101 ttttaatcat tttgacatct ccctcggcaa actattttat ttaattacct ttttaaaact
     8161 aaaactatat acgaccactt aactttgcac gataaatcat aatcttaaaa gcaaaagaaa
     8221 aagcccttgc acagcctgaa actgcacaag ggacgaatat ccgtgttgcc accctcattg
     8281 gtttaaaaaa catgcctaat tgtaatcatc attacttttt ataagcgtgc atttaataaa
     8341 cccactcaaa acgtttaacg tacgtttaac ggctttactt ttcataaagc acctcttctt
     8401 gtgttcaatg tatataaact actcagcata atctttcagt ctatggatta tattcctaca
     8461 caatcttagt tagattgcta cctatttcat ttaccatttc aagaatacta acggttttag
     8521 ttataaaatt caatatcaat gataactact ttcacatatt tttgcaagcc ctacgcagaa
     8581 aaataaattt taaggccatc aacaattgct tgttctaaaa tttgtctatg taattgatct
     8641 ttaatcatcg tttcatcagt tgggttacta atataaccta attctaataa aacagcagga
     8701 acttttgttt gtcttaacac ttgataattt tcttgtcttg aaccgcgatt agaaagtaga
     8761 cctttcttct gaatcgtagc gtctaacgta tctgctaaag ctctttgatt atcatgatac
     8821 caataaactg tcattccatt tgcattagat gattctaacg catcattatg tatactcaaa
     8881 taggcatcgc ctttgatatc acgattttct agtgaaacat atgtatcgtc tgttcttgtc
     8941 atcttaacag ttgcgccttc tttttctaaa gtacgctgca attcttttgc tgttttcaac
     9001 gtataatctt tttctaaact tttatattta gtattgcttg aagcaccctg gtcactacct
     9061 ccatgaccag gatcaagcac tattgtttta ccttgcaaag gatttttctc cttcgtatta
     9121 tccgcgacaa tatctaaatt tgtgtgccat ccagctatcc aacctttttc attactggat
     9181 gtatcttcaa cttcaatcca tttacctact ttaccaatct ttttaaaatg gtcacctttt
     9241 tcaactttat atatgactgg atacgcagcg tttggacctg tacgtaattc agcattttca
     9301 gttatcgtga tgttcccact atcttcacta ttgctattca gcaataaaaa taaaaagatg
     9361 ataaataaga caaaggcaat cactactatt agagtacgtt tatttttaag acccttttta
     9421 gataaccatg cctctatttt tttcattgaa ttttgccgtc ctgactttca taaataatag
     9481 tgactggacc atcattattt atgctaacat tcatgtgtgt tccaaattca cctgttttca
     9541 cagtaagacc atacgctcgt agcgcatcat taaaatactc ataaattttt accgcttgat
     9601 caggattttt agaatttgag aaacctggac ggttaccttt ttttacatct gcatagagag
     9661 taaattgtga aactgatagt atttcaccat tcatttgttg gatattaaag tttaatttat
     9721 tattgtcatc ttcaaataat cttgcattag caattttctt tgcaattaca tctgcatctt
     9781 gctctgtaga gttctgaccg ataccgacta ataaacaata tccttttttg atttgattat
     9841 ttaatgtatc attcgtcacc gatgcttctt taactctttg tacaactact ttcatatttt
     9901 gcacctctag ttccaaactc ttgttactgt ataaacatca ccaagttgtt tgatcttttc
     9961 taccacacga taaacatcat tcacgttttt caccatgaca ctaatattta ttattgcatt
    10021 tttatcaata tctgaacgtc ctgaaacttt aattaaattg ccggctgtcg agctaacagc
    10081 ttgtagtact tcattcaaca agccatttcg gtcatacgca gttacctcta aatcaacctg
    10141 atatttttga gttgcgtctt ttgattttac ccattcaaca ttaattagtc gttcagtttc
    10201 gttcttaata tttgggcaat cagtgcgatg tactttaata ccgtgacctt tggtgatata
    10261 acctacaata tcatcacctg gtataggatt acaacatttt gacaacttga taagtacatt
    10321 ttctaaacct tctacataga caccactatc agtaatgatg ttgtctttaa taggcaatga
    10381 tttcgtaact tcttgtgctt catttaaagc acgttgttta tctaaaatac gttgtctttc
    10441 agttaattta ttaacaatct gtaaggatgt cacgccgcca aatcctacag ctgcgaataa
    10501 atcatcttca tttgcaaagt tatatttttc attaacaacc tgaatatttt tctctgtcaa
    10561 aatatcttcg actctaaatc cttgctcttt tatttcagct tcaaccatca ttcggccttt
    10621 ttcaatatta gatgaacgat cttgtttttt gaagaaactt ttaattttac ctttggcact
    10681 agacgattta acaattttca accaatcacg acttggtcca tatgaatgtt tactagtacg
    10741 tatttcaaca atatcgcctg tttgtaaaat atagtcaatt ggtacaattt tgccattcac
    10801 cttggcacca atcatcttat tacctacttc actgtgaatc gcataagcaa aatcaatcgg
    10861 cacagcacca tatggcaact caataacatc actcgctggg gtaaatgcgt atactttgtc
    10921 actctgtaag tcatatttta aggtttccat aaattcttga gcgtcagacg atgtatgatc
    10981 cgcttcagct aattctttta accaatttaa cttattttga taagtttgat ctttttcact
    11041 tactttttta ccttctttgt aagcccagtg tgctgcaaca ccatgctcag caatttcgtg
    11101 catatcaaac gttcgtattt ggatttcgag cgggtctcca tttgggccta ctactgtagt
    11161 atgcaatgac tgatacaaat tttgtttagg cattgcaata taatctttaa aacgtcctgg
    11221 catcggtttc cataacgtat gcaccaaccc aagtatcgca taacaatcat taatagaatt
    11281 gacaataaca cgtatcgcca acaaatcaaa aatttgatca aattgttttt tctgcttcat
    11341 cattttccga taaatactgt aaatatgttt aggtctacca tttatatcgc cttcgatatt
    11401 cattcggtcc atttcagtac gtattctatc aatagccgtt tcgatatacg cttcacgttc
    11461 actacgtttc ttcttcatta aattgactat tctaaaatat tgcacattat caatataacg
    11521 aagagccgta tcttctagtt cccatttgat tgtattaata ccaagacgat gtgctaatgg
    11581 tgcataaatt tctaatgttt ctcgagaaat tctaatttgt ttttcgcgcg gcatggcttt
    11641 caaggtacgc atattatgta atctgtctgc taatttcacc aaaattacgc gtacatcttt
    11701 ggcaatcgca ataaataact tgcgatgatt ttcagcttgt tgttcttctt ttgagcggta
    11761 ttttactttt ttaagcttcg tcacaccatc aacaattcga gcaacttctt cattgaacat
    11821 ttcttttaca tcttcaaatg tatacggtgt atcttcaatt acatcatgca aaaaacctgc
    11881 gacaatcgtc ggtccgtcta atcgcatttc tgttaaaata cctgcaactt gtataggatg
    11941 cataatgtat ggtaatccgt tttttcggaa ctgaccttta tgtgcttcat aagcaatatg
    12001 atagcttttt aaaacatact catattcatc tgctgacaaa tatgattttg ctttgtgaag
    12061 aacttcgtct gcactatatg gatattcgtt gttcattata tgatacaccc cattcatatt
    12121 tattacttcg ccttaaacaa tgatttaggt actcttgttg aatagtattt gtcccacacc
    12181 aatcatacgt ccgtcgacga taaatattta tcctgtcgtc attaatcgta atattaattt
    12241 tacttgagcg agtttaattt gtatactatt cctactttta aaacttttac aaaaattcga
    12301 cctaaatcta ctgtttcatt ttttaaatat tagttctatg atactacaat ttatgaaata
    12361 aataaacgat gttattaagg tataatgctc aatcatctaa cattttcagt aaataaaaaa
    12421 tcctaaca
//
LOCUS       DAXWFK010000075        12003 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.075, whole genome shotgun sequence.
ACCESSION   DAXWFK010000075 DAXWFK010000000
VERSION     DAXWFK010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12003
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.075"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..1302
                     /gene="xseA"
                     /locus_tag="ACGEXS_001541"
     CDS             <1..1302
                     /gene="xseA"
                     /locus_tag="ACGEXS_001541"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFF8012552.1"
                     /translation="YIKYKFDQDPHLQSVLIKGELSNFKKHSSGHLYFNVKDKESVIS
                     AMMFKGSASKLNFEPKEGDEVLLEARVSVFERRGNYQIYVNKMQLDGIGNLYQKLEAL
                     KKKLTEEGCFDKANKKSIPKFPKKIAVLTASTGAAIRDIHSTINSRFPLAEQIQISTL
                     VQGEKAKDDIIEKIEYADSLGVDTIIVGRGGGSIEDLWNFNEEAVVRAIYNCKTPIIS
                     AVGHETDFTLSDFAADIRAATPTQAAVIATPDQYELLQQIQQYQFTLTRFIKKHLEQQ
                     RKHVEHLSSYYKFKQPTLLYDQQIQRRDDLEKRLKQQIQATFEQQRHRLMLLQQRYNL
                     KALLSSVNQEQQNNLQLTNQLVKLLNSKILSYKNDLKNKVENLNNLSPTNTMLRGYAI
                     VNKKDEVITSTKDLTENDQLTLTMKDGLVDAKVTKVRCNND"
     gene            1295..1525
                     /locus_tag="ACGEXS_001542"
     CDS             1295..1525
                     /locus_tag="ACGEXS_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFF8012553.1"
                     /translation="MTKETQSFEEMMQELEQIVQKLDNETVSLEESLDLYQRGMKLSA
                     ACDTTLKNAEKKVNDLIKEEAEDVKNDESTDE"
     gene            1503..2384
                     /locus_tag="ACGEXS_001543"
     CDS             1503..2384
                     /locus_tag="ACGEXS_001543"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFF8012554.1"
                     /translation="MTNLPMNKLIDEVNNELSVAINKSVMDTQLEESMLYSLNAGGKR
                     IRPVLLLLTLDSLNTEYELGMKSAIALEMIHTYSLIHDDLPAMDNDDYRRGKLTNHKV
                     YGEWTAILAGDALLTKAFELISSDDRLTDEVKIKVLQRLSIASGHVGMVGGQMLDMQS
                     EGQPIDLETLEMIHKTKTGALLTFAVMSAADIANVDDTTKEHLESYSYHLGMMFQIKD
                     DLLDCYGDEAKLGKKVGSDLENNKSTYVSLLGKDGAEDKLTYHRDAAVDELTQIDEQF
                     NTKHLLEIVDLFYSRDH"
     gene            2816..3268
                     /gene="argR"
                     /locus_tag="ACGEXS_001544"
     CDS             2816..3268
                     /gene="argR"
                     /locus_tag="ACGEXS_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFF8012555.1"
                     /translation="MPKKSVRHIKIREIISNEQIETQDELVKRLNDYDLNVTQATVSR
                     DIKELQLIKVPIPSGQYVYSLPNDRKFHPLEKLGRYLMDSFVNIDGTDNLLVLKTLPG
                     NAQSIGAILDQINWEEVLGTICGDDTCLIICRSKEASDEIKSRIFNLL"
     gene            3284..4963
                     /gene="recN"
                     /locus_tag="ACGEXS_001545"
     CDS             3284..4963
                     /gene="recN"
                     /locus_tag="ACGEXS_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFF8012556.1"
                     /translation="MLQTLSIKQFAIIEELEIQFSDGLTVLSGETGSGKSIIIDAIGQ
                     LIGMRASSDFVRHGEKKAVIEGIFDIDESKDAIHILKNMDIDVDEDFLLVKREIFSSG
                     KSLCKINNQTVTLQDLRKVMQELLDIHGQHETQSLLKQKYHLTLLDNYAESRYQDLLD
                     KYHQTFQNYKAKKQELEDLESADQALLQRLDLMKFQLEELSEAHLKEGEIEQLEIDIK
                     RIQNSEKLSLALNNAHMTLTDENAITDRLYELSNHLLTINDIVPNKYDKLKEDIDQFY
                     YILEDAKHELYDEMANTEFDEQVLNEYESRMNLLNNLKRKYGKDISELIAYQEKLNNE
                     INKIENYEQSTSQLREEINALYNQVIEVGQALSKQRRIVARELRDHIVSEIQNLQMKD
                     ANLEISFKKLEEPNIDGIEFVEFLISPNKGEPLKSLNKIASGGELSRIMLALKSIFVK
                     SRGQTAILFDEVDSGVSGQAAQKMAEKMRDIAEYIQVICISHLPQVASMSDHHLLISK
                     SSKDDRTTTQVQELISDDKVDEIARMISGASVTDLTRENAREMIQHNQRRR"
     gene            5114..6535
                     /gene="lpdA"
                     /locus_tag="ACGEXS_001546"
     CDS             5114..6535
                     /gene="lpdA"
                     /locus_tag="ACGEXS_001546"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFF8012557.1"
                     /translation="MSEKQYDLVVLGGGTAGYVAAIRASQLGKKVAIVERQLLGGTCL
                     HKGCIPTKSLLKSAEVFQTVKQAAMFGVDVKDANVNFENMLARKEDIINQMYQGVKHL
                     MQHNHIDIYNGTGRILGTSIFSPQSGTISVEYEDGESDLLPNQFVLIATGSSPAELPF
                     LSFDHDKILSSDDILSLKTLPSSIGIIGGGVIGMEFASLMIDLGVDVTVIEAGERILP
                     TESKQASQLLKKSLSARGVKFYEGIKLSENDINVNEDGVTFEISSDIIKVDKVLLSIG
                     RKPNTSDIGLNNTKIKLSTSGHILTNEFQQTEDKHIYAAGDCIGKLQLAHVGSKEGVV
                     AVDHMFEGNPIPVNYNMMPKCIYSQPEIASIGLNIEQAKAEGMKVKSFKVPFKAIGKA
                     VIDSHDTNEGYSEMVIDQSTEEIVGINMIGPHVTELINEASLLQFMNGSALELGLTTH
                     AHPSISEVLMELGLKAESRAIHV"
     gene            6551..7543
                     /locus_tag="ACGEXS_001547"
     CDS             6551..7543
                     /locus_tag="ACGEXS_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HFF8012558.1"
                     /translation="MIDYKSLGLSEEDLKVIYKWMDLGRKIDERLWLLNRAGKIPFVV
                     SGQGQEATQIGMAYALEEGDITAPYYRDLAFVTYMGISAYDTFLSAFGKKDDVNSGGK
                     QMPSHFSSRAKNILSQSSPVATQIPHAVGAALALKMDGKKKIATATVGEGSSNQGDFH
                     EGLNFAGVHKLPFVCVIINNKYAISVPDSLQYAAEKLSDRALGYGIHGEQVDGNDPLA
                     MYKAMKEARDRAISGQGSTLIEAVTSRMTAHSSDDDDQYRTKEERETLKKADCNEKFK
                     KELLSAGIIDDAWLAEIEAEHKDIINKATKAAEDAPYPSVEEAYAFVYEEGSLN"
     gene            7543..8526
                     /locus_tag="ACGEXS_001548"
     CDS             7543..8526
                     /locus_tag="ACGEXS_001548"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFF8012559.1"
                     /translation="MAKLSYLEAIRQAQDLALQQNKDVFILGEDVGRKGGVFGTTQGL
                     QQKYGEDRVIDTPLAESNIVGTAIGAAMVGKRPIAEIQFADFILPATNQIISEAAKMR
                     YRSNNDWQCPLTIRAPFGGGVHGGLYHSQSIESIFASSPGLTIVIPSTPYDAKGLLLS
                     SIESNDPVLYFEHKKAYRFLKEEVPEEYYTVPLGKADVKREGEDLTVFCYGLMVNYCL
                     QAADILAADGINVEVVDLRTVYPLDKETIIDRAKHTGKVLLVTEDNLEGSIMSEVSAI
                     IAEHCLFDLDAPIMRLAAPDVPSMPFSPVLENEIMMNPEKILNKMRELAEF"
     gene            8539..9813
                     /locus_tag="ACGEXS_001549"
     CDS             8539..9813
                     /locus_tag="ACGEXS_001549"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HFF8012560.1"
                     /translation="MEITMPKLGESVHEGTIEQWLVSVGDHIDEYEPLCEVITDKVTA
                     EVPSTISGTITEILVEAGQTVAIDTIICKIETADEKTNETTEEIQAKVDEHTQKSTKK
                     ASATVEQTSTAKQNQPRNNGRFSPVVFKLASEHDIDLSQVVGSGFEGRVTKKDIMSVI
                     ENGGTTAQSDKQVQTKSTSVDTSSNQSSEDNSENSTIPVNGVRKAIAQNMVNSVTEIP
                     HAWMMIEVDATNLVNTRNHYKNSFKNKEGYNLTFFAFFVKAVADALKAYPLLNSSWQG
                     NEIVLHKDINISIAVADENKLYVPVIKHADEKSIKGIAREINTLATKARNKQLTAEDM
                     QGGTFTVNNTGTFGSVSSMGIINHPQAAILQVESIVKKPVVINDMIAIRNMVNLCISI
                     DHRILDGLQTGKFMNHIKQRIEQYTLENTNIY"
     gene            10164..10601
                     /gene="brxB"
                     /locus_tag="ACGEXS_001550"
     CDS             10164..10601
                     /gene="brxB"
                     /locus_tag="ACGEXS_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacilliredoxin BrxB"
                     /protein_id="HFF8012561.1"
                     /translation="MDMNFDLYMNGVVEQARNEIESAGYEQLTTAEDVDKVLKQDGTT
                     LVMINSVCGCAGGIARPAASHALHYDVLPDRLVTVFAGQDKEATQRAREYFEGYAPSS
                     PSFALVKDGKITEMIERHQIEGHDVMNVINQLQTLFNKYCEER"
     gene            10615..11595
                     /locus_tag="ACGEXS_001551"
     CDS             10615..11595
                     /locus_tag="ACGEXS_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFF8012562.1"
                     /translation="MLKLNPYKIGFRTIKTAVGMTLGVIISKLLGLDNYASSAILVVL
                     CIKHTKVHSLQAIISRLVSCFLVLFLGSAIFSLLGQSPIVLGIIVLLFIPLTVVLKVQ
                     EGVITSCVILLHVFNAKSIDAHLIVNETLLLLIGLSIAFTMNLMMPSLDKQLDEYKCK
                     IEQQIADIFSKYSYICEKYEDTIAIEFEVLLLNIKKAKSIAFRDVKNHFVRNENSYYH
                     YFDMREEQVELLMRMKPLIESICHKDPLLNDVASLLKEIGLNVNSNDYTAMRLHNLYE
                     TRLKLDELPLPTTHKALNSRASIIQILNELEEYLQIKSQFGSLKMHSEVA"
     gene            complement(11722..11901)
                     /gene="prli42"
                     /locus_tag="ACGEXS_001552"
     CDS             complement(11722..11901)
                     /gene="prli42"
                     /locus_tag="ACGEXS_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stressosome-associated protein Prli42"
                     /protein_id="HFF8012563.1"
                     /translation="MLVISSQVKSFISYVVIINTKVIHLEAHKMQNKVLRIIIIVMLV
                     SVVLALLLTSIIPIL"
BASE COUNT         4490 a         1634 c         2254 g         3625 t
ORIGIN      
        1 tatattaaat ataaatttga tcaagatcca catctccaat ctgtattgat taaaggtgag
       61 ctttcaaatt tcaaaaagca ttcaagtggt catttatact ttaatgtgaa agataaagaa
      121 agcgttataa gtgccatgat gttcaaaggt agtgcttcta aattaaactt cgaacctaaa
      181 gaaggagatg aagtcttatt agaagcacgt gtttctgttt ttgaacgtcg tggaaactat
      241 caaatttatg taaataaaat gcaattagat ggtataggga acttatatca aaaattagaa
      301 gcattaaaga aaaaattaac tgaagaaggt tgttttgata aagcgaataa gaaatcaata
      361 cctaagtttc ctaaaaaaat tgcagtttta acagcgagta caggtgccgc aattcgagat
      421 atccattcaa cgataaatag tcgttttcca ttagctgaac aaatacaaat tagtactttg
      481 gttcaaggtg aaaaagcaaa agacgacatt attgaaaaaa ttgaatatgc agatagttta
      541 ggtgtagata ccattattgt aggtcgaggc ggtggatcca tagaagattt atggaatttc
      601 aacgaagaag ctgtcgtccg tgcaatatat aattgtaaga caccgattat atcagcagtt
      661 ggtcatgaaa cagactttac attaagtgat tttgctgcag acatcagagc tgcgactcca
      721 actcaagctg ctgttattgc aacaccagac caatatgaat tgctgcaaca aatacagcaa
      781 tatcagttta cattgactcg tttcatcaag aaacatttag aacaacaacg taaacatgtt
      841 gaacatttgt catcgtatta caagtttaaa caaccaactt tattgtatga tcaacagata
      901 caacgtcgag atgatttaga aaagagactg aaacaacaaa ttcaggcaac ttttgaacaa
      961 cagagacatc gattaatgct gttgcaacaa cgttataatt taaaagcatt actaagtagt
     1021 gttaatcaag aacaacaaaa taatcttcaa ttgacaaacc aacttgttaa attattgaat
     1081 tctaagatat taagttataa aaatgatttg aaaaataaag tagaaaactt aaacaattta
     1141 agtccaacta atacaatgtt gcgtggatat gcaattgtta ataaaaaaga cgaagtcatt
     1201 acgagtacta aagatttaac agaaaatgat caattgacgt taacaatgaa agatggctta
     1261 gtagatgcaa aagttacgaa agtaaggtgt aataatgact aaagaaacgc aaagttttga
     1321 agaaatgatg caagaattag agcaaattgt tcaaaaatta gataatgaaa cagtatcttt
     1381 agaggaatca ttagatttat atcaacgtgg tatgaaacta tcagcagctt gtgacacaac
     1441 tttaaaaaat gccgaaaaaa aggtgaatga cttaataaaa gaagaagctg aggatgtaaa
     1501 aaatgacgaa tctaccgatg aataaattaa tagatgaagt caataatgaa ttatcggttg
     1561 cgataaataa atcagtaatg gatactcagc tagaagaaag tatgttgtat tcattaaatg
     1621 ctggaggtaa acgcatccga ccagttctgt tattactcac tttagattca ctaaataccg
     1681 agtatgagtt aggtatgaag agcgcaattg cactagaaat gattcataca tattcactta
     1741 ttcatgatga cctaccagcg atggataatg atgattatcg acgaggaaaa ttaacaaatc
     1801 ataaagtata tggtgagtgg actgcgatat tagcaggtga tgctttatta actaaagcat
     1861 ttgaacttat ttcaagtgat gatagattaa ctgatgaagt aaaaataaaa gttctacaac
     1921 ggctgtcaat agcaagtggt catgttggaa tggtcggcgg tcaaatgtta gatatgcaaa
     1981 gcgaaggcca accaattgat cttgaaactt tggaaatgat acacaaaaca aaaacaggag
     2041 cattattaac ttttgcggtt atgagtgcag cagatatcgc taatgtcgat gatacaacta
     2101 aagaacattt agaaagttat agttatcatt taggtatgat gttccagatt aaagatgatt
     2161 tattagactg ctatggtgat gaagcaaagt taggtaaaaa agtgggcagc gatcttgaaa
     2221 ataataaaag tacgtacgtg agtttattag ggaaagatgg cgcagaagat aaattgactt
     2281 atcatagaga cgcagcagtg gatgaactaa cgcaaattga tgaacaattc aatacaaaac
     2341 acttattaga aatcgttgat ttattttata gcagggatca ctaaaagcaa atatatcgat
     2401 tagcaacaat tgtaaagttt gaaatgtctc aaaaggcata ttcaactgac gttgtttgtt
     2461 aatcgatttt tctcatgttg aaacttaaaa ttatgttgaa gactttcatt taaaaaatag
     2521 aattttcaat ttctatacga caatattcgt atctgtgata tcaatgtgat tttaattaaa
     2581 tatgttcaaa caattgacgc ttattaaatc taataattag caagtttatt acacatatat
     2641 tcatttttat acatatttta agcaattgtt atgcgtaata tgaaaaatat ttataaatag
     2701 ataaccaaga tagataaacc atgtttaccc tttttacgta aaggtgttta gtagtcgtaa
     2761 atcagtgcat gtgttaaaat aaatgtataa atattcgtaa tcagaggtgt ttactgtgcc
     2821 caaaaaatcg gttaggcata taaaaattag agaaattatt tcaaatgaac agatagagac
     2881 acaagatgaa ttagttaaac gattaaacga ttatgattta aatgtcactc aagcaactgt
     2941 ttctcgtgat attaaagaac tacaacttat taaagtacct ataccttcag gtcaatatgt
     3001 ttatagttta ccaaatgata gaaaattcca tcctttagaa aaattgggac gttatttaat
     3061 ggattccttt gttaatatag atggtactga taatttactt gttctaaaaa cattacctgg
     3121 taatgcacaa tctattggag ctatattaga ccaaatcaat tgggaagaag tactaggcac
     3181 aatttgtggt gatgatactt gtttaattat ttgtcgaagc aaagaggcaa gtgatgaaat
     3241 caagtcaaga attttcaatt tgttataagg atgcgatgag tttatgttac aaaccttatc
     3301 aatcaagcaa tttgctatta ttgaagaatt agaaattcaa ttttctgatg gtttaactgt
     3361 tttaagtggt gaaacgggtt caggtaagtc aattattata gatgcaattg gacaattaat
     3421 tggaatgaga gcttcatctg attttgtaag acatggtgaa aaaaaggctg tcatcgaagg
     3481 tatatttgat attgacgaga gtaaagatgc tatacatata ttgaaaaata tggatattga
     3541 tgtagatgaa gattttttat tagttaaacg ggaaattttc agctctggta aaagtctttg
     3601 taaaattaat aatcaaacag ttactttaca agatttacga aaagttatgc aagaattatt
     3661 agatattcat ggccaacatg aaacgcagtc tttgttgaaa caaaaatacc atttaacatt
     3721 attggataat tacgcagaat cacgttatca agatttgttg gataaatatc accaaacttt
     3781 tcaaaattat aaagccaaaa agcaagagtt agaagattta gaatcagcag accaagcatt
     3841 gctacagcgt ttagatttaa tgaaattcca attagaagaa ttgtctgaag cacatctaaa
     3901 agaaggcgaa atagagcaac ttgaaataga tattaaacgc attcaaaact ctgaaaaatt
     3961 aagcttagcg cttaataacg cacatatgac attaacggat gaaaatgcta tcactgatcg
     4021 tttatatgaa ctaagcaacc atttgttaac aataaacgat atagttccaa acaaatatga
     4081 caaattaaaa gaagatattg atcaatttta ctacatttta gaagatgcaa aacatgagtt
     4141 atatgatgaa atggctaata cagaattcga cgaacaagtg ttaaacgagt atgaatctcg
     4201 tatgaatttg ctgaataatt taaaacgtaa atatggaaaa gatatttcag aattaatcgc
     4261 atatcaagaa aagcttaata atgaaatcaa taaaattgaa aactatgaac aaagtacatc
     4321 gcagttacga gaagaaataa atgcattgta taatcaagtt atagaggttg gacaagcgtt
     4381 atcaaagcag cgtcgtatag tcgctaggga gttaagagac cacattgtat cggaaattca
     4441 aaacttacaa atgaaagacg caaatcttga gatttcattt aaaaaattag aagaaccgaa
     4501 tattgatgga atcgaatttg tagaattttt aatcagtcca aataaagggg aaccattaaa
     4561 aagtttaaat aaaattgcgt caggtggaga actttctaga attatgttag cgttaaaaag
     4621 tatttttgtt aaatcgagag gtcaaactgc aattttattc gatgaggttg actcaggtgt
     4681 atctggacaa gctgcacaaa aaatggcaga gaaaatgcga gatatagcag aatatataca
     4741 agttatttgt atctctcact taccacaagt tgcatcgatg agcgaccatc atttattaat
     4801 ttcgaaatca tcaaaagatg atcgtacaac aacacaagtt caagagttaa ttagcgatga
     4861 taaagtagat gaaattgcac gaatgatttc aggtgcaagt gttactgatt taactcgaga
     4921 aaatgcaaga gaaatgatac aacacaatca aagacgtaga taatttccaa agtgtgttga
     4981 atactcattt atggatttat ttatgtgcat tcaaactaaa tagacaatca tgtaaaatag
     5041 gtaattaatg gacatgaaaa ctatgtatta ataaattttc aatggatata tatcattagt
     5101 taggagtaag aacatgtcag agaaacaata tgatttagtc gttctcggtg gaggtactgc
     5161 aggatatgtt gctgcgatac gtgcgtctca attaggtaaa aaagtggcaa tagtagaacg
     5221 acaactatta ggaggtactt gtctccataa aggttgtatt cctactaaat cgctgcttaa
     5281 atctgctgaa gtatttcaaa cagtaaaaca ggcagcaatg tttggcgttg atgtcaaaga
     5341 tgctaatgtt aattttgaaa atatgttggc acgaaaagaa gacattatta atcaaatgta
     5401 tcaaggtgta aagcatttaa tgcaacacaa tcacattgac atttataatg gcacgggacg
     5461 tattctaggt acatctatat tttcacctca aagtggcaca atttctgtcg aatatgaaga
     5521 tggtgaatca gacttattac ccaaccaatt tgtattaatt gcaactggtt cttcgcctgc
     5581 agagttgcca ttcttatcat ttgatcatga taaaatttta tctagcgatg atatattatc
     5641 attaaagacg ttaccatcaa gtattggtat tattggtggt ggtgttatcg gaatggaatt
     5701 tgcatcattg atgatagatt taggtgttga cgtaacagtt atagaagcag gtgaaagaat
     5761 tttaccaact gaaagtaaac aagcttcaca actattaaaa aaatcattgt cagcacgtgg
     5821 tgttaaattc tatgagggaa taaaactttc tgaaaatgat ataaatgtta acgaagatgg
     5881 tgttacgttt gaaatttcct cagacataat taaagtagat aaagtgttgc tatcgattgg
     5941 tagaaaacct aacacatcag atattggttt aaacaacact aaaataaaac tttcaacatc
     6001 aggacatatt ttaacgaacg aatttcaaca aactgaagat aaacatattt atgcagctgg
     6061 tgattgtata ggaaaattac aattggcaca tgttggatca aaagaaggtg ttgtggctgt
     6121 tgatcatatg tttgagggga acccaatccc agtaaactat aacatgatgc ctaagtgtat
     6181 ttattcacaa cctgaaattg cttctatcgg tttaaacatt gaacaagcaa aggcagaggg
     6241 aatgaaagtt aaaagtttta aagtaccatt taaagcaatt ggtaaagcag tgattgatag
     6301 ccatgacaca aacgaagggt atagcgaaat ggtgattgat caatcaactg aagaaattgt
     6361 gggtattaat atgattggtc cacatgtaac agaattgatt aatgaggcat cactgttaca
     6421 gttcatgaat ggctcggcat tagaattagg actaacaaca cacgcacatc cttccatctc
     6481 tgaagtgttg atggaattag gattgaaagc agaaagtaga gctattcacg tataaatagg
     6541 aggatttaac atgattgatt ataaatcatt aggccttagc gaagaagacc taaaagtaat
     6601 atataaatgg atggatttag gtagaaaaat agatgaaaga ctgtggttat tgaaccgtgc
     6661 aggtaaaatt ccatttgttg taagtggtca aggacaagaa gcaactcaga ttgggatggc
     6721 atacgcatta gaagaaggtg atattacagc gccttattat agagatctag ctttcgttac
     6781 ttatatgggt atctcagcgt atgatacgtt tttatcagcg tttggaaaga aagacgatgt
     6841 taactctggt ggaaaacaaa tgccatcaca ctttagtagt agagctaaga atattttgtc
     6901 tcaaagttca ccggtagcaa ctcaaatacc ccatgctgtt ggtgctgctt tagctttaaa
     6961 aatggatggt aagaagaaaa ttgcaacagc aactgttggt gaaggtagct caaaccaagg
     7021 tgacttccat gaaggtttaa acttcgcggg tgtacacaaa cttccttttg tatgtgtcat
     7081 tataaataat aaatatgcaa tttcagtacc tgattcatta caatacgcag ctgaaaaatt
     7141 atcggataga gcacttggtt acggtataca tggtgaacaa gtagatggta atgatccatt
     7201 agcaatgtat aaagcgatga aagaagcaag agatcgagct atttctggtc aaggttcaac
     7261 tttgattgaa gcagtaacaa gtcgtatgac agctcattca tcagatgatg acgaccaata
     7321 tcgtacgaaa gaagagcgtg aaacgcttaa aaaagcagac tgcaatgaaa agttcaaaaa
     7381 agagttgctt tcagctggca ttatcgatga tgcttggttg gcagaaatag aagcggagca
     7441 taaagacatc atcaataaag cgacaaaggc tgctgaagac gcgccatatc caagtgtcga
     7501 agaagcctat gcatttgtgt acgaagaagg gagtcttaac taatggctaa attatcttat
     7561 ttagaggcga tacgccaggc acaagactta gcattacaac aaaataaaga tgtgtttatt
     7621 cttggtgaag atgttggtag aaaaggtggc gtatttggta ctactcaagg actgcaacaa
     7681 aaatatggtg aagacagagt tatcgatacg ccattagctg agtcaaatat tgttggaact
     7741 gccattggtg cagctatggt aggtaaaaga ccaattgcag aaattcaatt tgcagatttt
     7801 attttacctg cgacaaatca aattattagt gaagctgcga aaatgcgtta tcgttcaaat
     7861 aatgattggc aatgcccatt aacgattcgt gcaccatttg gtggcggtgt tcacggagga
     7921 ctatatcatt cacaaagtat tgaaagtata tttgcttcat cgccaggttt aacaattgtt
     7981 attccttcca caccgtatga tgctaaaggg cttttattat catctatcga atcaaatgat
     8041 ccagtattgt actttgagca taaaaaagct tatcgtttct taaaggaaga agtgccagaa
     8101 gaatattaca ctgtaccttt aggaaaagca gatgtgaaac gtgaaggtga agatttaaca
     8161 gtattttgtt atggattaat ggtcaattat tgtttacaag cagcggatat tttagctgca
     8221 gacggtatca atgttgaagt cgttgattta cgaactgttt atccattaga taaagaaaca
     8281 attattgatc gtgctaaaca tacaggtaaa gttttacttg taactgaaga taatttagaa
     8341 ggtagtatta tgtcagaagt gtcagcgatt attgcagagc attgcttgtt cgatttagat
     8401 gcaccaatca tgcgtttagc tgctccagat gtaccatcta tgccattttc tcctgtatta
     8461 gaaaatgaaa ttatgatgaa tccagaaaaa atcttaaata aaatgcgtga attagcagaa
     8521 ttctagggag ggaaagtcat ggaaataaca atgcctaagt taggtgagag tgttcatgaa
     8581 ggcaccattg aacaatggtt agtttctgtt ggtgatcata ttgatgaata tgaaccatta
     8641 tgtgaagtta ttacagataa agtgacagct gaagtccctt ccacgatatc aggaacaatt
     8701 acagaaattt tagttgaagc ggggcagaca gtagctattg atacaattat ctgtaaaatt
     8761 gaaactgctg atgaaaagac aaatgaaaca actgaagaga tacaagcaaa agtggatgag
     8821 catactcaga aatctactaa aaaagctagt gcaacagtgg aacagacatc tactgctaaa
     8881 caaaatcaac cacgtaataa tggtcgcttt tcacctgttg tatttaaact cgcttcagag
     8941 catgacattg atttatcaca agttgtaggt agtggatttg aaggtcgtgt aactaagaag
     9001 gatataatgt cagttattga aaatggtggt accacagctc aatctgacaa acaagttcaa
     9061 acaaaatcaa catcagtaga tacatcaagt aaccaatcat ctgaagacaa tagtgaaaac
     9121 agcacaatac cagtaaatgg tgtgcgtaaa gcaattgcgc aaaatatggt taatagtgta
     9181 acagagattc cacatgcatg gatgatgatt gaagtagatg ctacaaatct tgtgaatacg
     9241 agaaatcatt ataaaaacag ctttaaaaat aaagaaggat ataatctaac gttctttgct
     9301 ttctttgtaa aagctgtagc agatgcttta aaagcatatc ctttattaaa tagtagctgg
     9361 caaggaaatg aaattgtctt acataaagac attaatattt caattgctgt tgctgatgaa
     9421 aataaattat acgtacctgt gattaagcat gcagacgaaa agtcaatcaa aggtatagct
     9481 agagaaatta atactttagc aacgaaagcg cgtaataagc aattgacagc tgaagatatg
     9541 cagggcggta catttacggt aaataatact ggtacatttg gttcagtatc atcaatgggt
     9601 attataaatc atccacaagc agcgatttta caagtagaat caatcgttaa aaagccagta
     9661 gtaattaatg atatgattgc aattcgtaac atggttaatt tatgtatttc aattgatcat
     9721 cgtattttag atggtttaca aacaggtaaa tttatgaatc atattaaaca gcgtatcgaa
     9781 cagtatactt tagaaaatac aaatatatat tagtgataac atagatgcat ctatcgacaa
     9841 cttgttttat cttgttcttg tcgatggatg tattattttt tggcactaaa atatgtgcaa
     9901 tatattcaaa aaagataaag aacaataatc aacatggttg aatgcatttt tgcagtaaag
     9961 tcaaaataag acatcatact tgaaacatat taatgaaaac atgtgaacaa attagttacc
    10021 atgattttaa gcacaataat gtttggtata ttgttaaaat tgtgtctaaa tataggtgtg
    10081 attcagatta gtttattgaa caatatgtta ttaattagta gaatgaggat agtttaaata
    10141 taaagggata ggtgattgaa cttatggaca tgaatttcga tttatacatg aacggtgttg
    10201 tagaacaagc aaggaatgaa attgaatctg cgggatatga gcaattaact actgcagaag
    10261 atgttgacaa agttcttaaa caagatggta caacactagt tatgatcaat tctgtatgtg
    10321 gttgtgcagg tggtatcgca agaccagcag catcacatgc tttacattat gacgtattac
    10381 ctgatcgtct agtgacagta tttgctggac aagataaaga agcgacacaa agagcgcgtg
    10441 aatacttcga aggttatgcg ccttcaagtc cgtcatttgc attagtaaaa gatggaaaga
    10501 ttacagaaat gattgaaaga catcaaatcg aaggtcatga tgtgatgaac gtaattaatc
    10561 aattacaaac attattcaat aaatattgtg aagaaagata agaggcgcta acccatgtta
    10621 aagttaaatc cttacaagat tggatttaga acaataaaaa cagcagtggg tatgacttta
    10681 ggtgtaatta ttagtaagct gttaggttta gataattatg cttcaagcgc catattagtc
    10741 gtattatgta ttaaacatac aaaagtacat tcgctacaag cgattatttc aagattagta
    10801 tcatgttttt tagtattgtt tttaggttca gcaatattta gtttattagg tcagagtcca
    10861 attgtactcg gtattatcgt attgttattt ataccattaa ctgtcgtatt aaaagtacaa
    10921 gaaggtgtca ttacgagttg cgttatatta cttcatgttt ttaatgcaaa atcaattgat
    10981 gcacatttaa ttgttaatga aacattatta ctgttaattg gactaagcat tgcatttaca
    11041 atgaatttaa tgatgccaag tttagacaaa caactagacg aatacaaatg taaaattgag
    11101 caacaaattg ctgatatttt tagtaaatat agttatattt gtgaaaaata tgaagatacc
    11161 attgcgattg aatttgaagt gttactttta aatattaaaa aggcgaagtc tatcgcgttc
    11221 cgagatgtta aaaatcattt tgttagaaac gaaaattcat actatcatta ttttgatatg
    11281 cgagaagagc aagtggaatt gttaatgaga atgaaaccgc tcatcgaaag tatctgtcat
    11341 aaagatccgc ttttaaatga tgttgcgtca ctgctgaaag aaattggtct aaatgtgaat
    11401 agtaatgact atacagcaat gcgtttacac aatttgtatg agacgcgttt gaaattggat
    11461 gaactgccat tgccaacaac acacaaagcg cttaactcga gagcaagtat tattcaaata
    11521 ttaaatgaat tagaagaata tttacaaatc aaatcgcaat ttggttcatt gaaaatgcat
    11581 agtgaagtag cttaaaaaat aatccatccg tatgacccat gtttgaaagt aatcgttgca
    11641 atacgataac ttattaatac atgcgtttat aatggatgga tttttaattg tatgattttg
    11701 tataggtagt tgagatatag tttataaaat tggaatgata ctcgttaata acaatgctaa
    11761 tacaactgat acaagcataa cgataatgat aattcttaaa actttatttt gcattttgtg
    11821 tgcctccaaa tgaataacct ttgtattaat aattacgaca taagatataa aacttttaac
    11881 ttgagacgat ataactagca atcatcataa aggaatataa taaaaaacat gagtaagttt
    11941 gcttatgtca ttttcctcat tatacgatat ctttgaaaaa tcaatcaata tatttttgac
    12001 aaa
//
LOCUS       DAXWFK010000076        11994 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.076, whole genome shotgun sequence.
ACCESSION   DAXWFK010000076 DAXWFK010000000
VERSION     DAXWFK010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11994
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.076"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(86..553)
                     /locus_tag="ACGEXS_001553"
     CDS             complement(86..553)
                     /locus_tag="ACGEXS_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HFF8012564.1"
                     /translation="MTIKVEDNKIIFSRTIEAPIEKVFDAYTTKALFEKWFHPKDAST
                     KVFRFNAVSGGDAFYAIKTPTMTSYTLAEYKTVKRPYLIEYIDSFATPQGAKDTKMPS
                     MKITLSFSKSNTTKTTVTSTSVFPTKEAAQQVINMGVEQGMNQTLDQLDALLK"
     gene            complement(739..2289)
                     /gene="adcA"
                     /locus_tag="ACGEXS_001554"
     CDS             complement(739..2289)
                     /gene="adcA"
                     /locus_tag="ACGEXS_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HFF8012565.1"
                     /translation="MKKKLGMLLLVPAVTLSLAACGNDDGKDKDGKVTIKTTVYPLQS
                     FAEQIGGKHVKVSSIYPAGTDLHSYEPTQKDILSASKSDLFMYTGDNLDPVAKKVAST
                     IKDKDKKLSLEDKLDKAKLLTDQHEHGEEHEHEGHDHEKEEHHHHHGGYDPHVWLDPK
                     INQTFAKEIKDELVKKDPKHKDDYEKNYKKLNDDLKKIDNDMKQVTKDKQGNAVFISH
                     ESIGYLADCYGFVQKGIQNMNAEDPSQKELTKIVKEIRDSNAKYILYEDNVANKVTET
                     IRKETDAKPLKFYNMESLNKEQQKKDNITYQSLMKSNIENIGKALDSGVKVKDDKAES
                     KHDKAISDGYFKDEQVKDRELSDYAGEWQSVYPYLKDGTLDEVMEHKAENDPKKSAKD
                     LKAYYDKGYKTDITNIDIKGNEITFTKDGKKHTGKYEYNGKKTLKYPKGNRGVRFMFK
                     LVDGNDKDLPKFIQFSDHNIAPKKAEHFHIFMGNDNDALLKEMDNWPTYYPSKLNKDQ
                     IKEEMLAH"
     gene            complement(2478..2549)
                     /locus_tag="ACGEXS_001555"
                     /pseudo
     CDS             complement(2478..2549)
                     /locus_tag="ACGEXS_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501155.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2584..2850)
                     /locus_tag="ACGEXS_001556"
     CDS             complement(2584..2850)
                     /locus_tag="ACGEXS_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFF8012566.1"
                     /translation="MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALE
                     GIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIKIASCKYHY"
     gene            complement(2850..3101)
                     /locus_tag="ACGEXS_001557"
     CDS             complement(2850..3101)
                     /locus_tag="ACGEXS_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFF8012567.1"
                     /translation="MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESD
                     YNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKDIDV"
     gene            complement(3266..3367)
                     /locus_tag="ACGEXS_001558"
     CDS             complement(3266..3367)
                     /locus_tag="ACGEXS_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012568.1"
                     /translation="MLRSVSDTNLLGQYEKAISISDMIEIVFLMYEV"
     gene            complement(3374..3973)
                     /locus_tag="ACGEXS_001559"
     CDS             complement(3374..3973)
                     /locus_tag="ACGEXS_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFF8012569.1"
                     /translation="MTKKLLTLFIVSMLILTACGKKESATTSSKNGKPLVVVYGDYKC
                     PYCKELDEKVMPKLRKNYIDNHKVEYQFVNLAFLGKDSIVGSRASHAVLMYAPKSFLD
                     FQKQLFAAQQDENKEWLTKELLDKHIKQLHLDKETENKIIKDYKTKDSKSWKAAEKDK
                     KIAKDNHIKTTPTAFINGEKVEDPYDYESYEKLLKDKIK"
     gene            complement(3992..4354)
                     /locus_tag="ACGEXS_001560"
     CDS             complement(3992..4354)
                     /locus_tag="ACGEXS_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4467 domain-containing protein"
                     /protein_id="HFF8012570.1"
                     /translation="MKRFVATVLLLLVFISGCGNDKYVKEIDEAVKIQNQKQEQLAKK
                     GNGDRVDHFERKDANIYVYDKDKIIILAYKPLSNDDEVHYYAYDFSDKRVSYKQDFDS
                     RRYYQQHDADYHEENMTN"
     gene            4609..5859
                     /gene="fmhA"
                     /locus_tag="ACGEXS_001561"
     CDS             4609..5859
                     /gene="fmhA"
                     /locus_tag="ACGEXS_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhA"
                     /protein_id="HFF8012571.1"
                     /translation="MNFVTLTSDEFNAFTTKHFSHYTQSAIHYNHRVDLKGDVHLVGV
                     KDDNGQVIAGCLLTEARTLKFFKYFYTHRGPVMDYTNQSLVAFFFKALTSYLKKHNCL
                     YVLVDPYLIENLRNADGEIVKSYDNRAFVRTMDKLGYKHQGFPVGYDSMSQIRWLSVL
                     DLKDKTEDQLLKEMDYQTRRNIKKTYDIGVKTKTLTIDETQTFFDLFHMAEEKHGFKF
                     RELPYFEEMQKLYDDHAMLKLAYIDLNEYLKTLQLKQQQLTAELSGVEEALEESPNSK
                     KNKTKRTQLEQQLNSNKRKIDNTIEQIEQDGAVLNLASALFIYNEHEVYYLSSGSNPK
                     YNAYMGAYHLQWEMIKFAKAHHIDRYNFYGITGDFSESSEDYGVQQFKKGFNAHVEEY
                     IGDFIKPIKPLLYKVQTYLNHKRR"
     gene            complement(5956..6687)
                     /locus_tag="ACGEXS_001562"
     CDS             complement(5956..6687)
                     /locus_tag="ACGEXS_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFF8012572.1"
                     /translation="MIQLNNIHKSFNDVEVIKGIDLSVEQGEVVTLIGRSGSGKTTLL
                     RMINALEIPTEGTVYVNGKTYTSKDKKSQIEVRKQSGMVFQSYNLFPHKTALENVMEG
                     LITVKKLKKDEARGKSLELLEKVGLTHVKDQRPHALSGGQQQRVAIARALAMNPKVML
                     FDEPTSALDPELVNDVLKVIKDLANEGMTMVIVTHEMRFAKEVSNNIVFIHEGMIGEQ
                     GAPEEMFNRPKTEELRRFLNVINEE"
     gene            complement(6684..7403)
                     /locus_tag="ACGEXS_001563"
     CDS             complement(6684..7403)
                     /locus_tag="ACGEXS_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFF8012573.1"
                     /translation="MFLNLNSEQQHALDAAKQAFGPMLEGLVKYSIPITLVTFVLGLI
                     IALFTALMRISTSKILRSISRVYVSIIRGTPMIVQLFIIFYGIPELGRLLTNDADNQW
                     TLAPVVAAIIGLSLNVGAYASEIIRGGIISIPKGQTEAAYSIGMTYGQTIQRIILPQA
                     IRVSIPALGNTFLSLIKDTSLLGFILVAEMFRKAQEVASTTYEYLTIYVLVALMYWVV
                     CFIISIIQGIYESYIERGYRS"
     gene            complement(7384..8163)
                     /locus_tag="ACGEXS_001564"
     CDS             complement(7384..8163)
                     /locus_tag="ACGEXS_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFF8012574.1"
                     /translation="MKRLLFVMIAFVFILAACGNNSSKDKEASKDSKTINVGTEGTYA
                     PFSFHDKDGKLTGYDIDVIKAVAKEEGLKLKFNETSWDSMFAGLDAGRFDVIANQVGI
                     NPDREKKYKFSKPYTFSSAVLVIRENEKDIKDFDDVKGKKLAQTFTSNYGKLAKDKGA
                     DITKVDGFNQSMDLLLSKRVDGTFNDSLSYLDYKKQKPNAKIKAIKGNAEQSRSAFAF
                     SKKADDETVQKFNDGLKKIEENGELAKIGKKWFGQDVSKSK"
     gene            complement(8284..9723)
                     /gene="mdeA"
                     /locus_tag="ACGEXS_001565"
     CDS             complement(8284..9723)
                     /gene="mdeA"
                     /locus_tag="ACGEXS_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdeA"
                     /protein_id="HFF8012575.1"
                     /translation="MSKKQKLTMIITMLMGGFFGLLNETLLVTALPSIMKDFEISYTQ
                     VQWLTTAFLLTNGIVIPLSALVIQRYTTRQVFLVGISIFFLGTLLGGLSPHFATLLVA
                     RIIQALGAGIMMPLMMTTILDVFQPHERGKYMGIFGLVIGLAPAIGPTLSGYLVEYFN
                     WRSLFHVVAPIAAVTFLIGFKTIKNVGTTIKVPIDFISVIFSVLGFGGLLYGTSSISE
                     KGFDNPIVLVSMIGGVVLVALFVLRQYRLSTPLLNFAVFKNKQFTVGIIIMGVTMVSM
                     IGSETILPIFVQNLLHRSALDSGLTLLPGAIVMAFMSMTSGALYEKFGPRNLALVGMA
                     IVVITTAYFVVMDEQTSTIMLATVYAIRMVGIALGLIPVMTHTMNQLKPEMNAHGSSM
                     TNTVQQIAGSIGTAALITILSHASKNFSPTMSDYNGMNKIDMMNQIKVDTMLHGYHAG
                     FLFALLITVVSFFCSFMLQGKKKEVDSRQ"
     gene            9897..10001
                     /locus_tag="ACGEXS_001566"
     CDS             9897..10001
                     /locus_tag="ACGEXS_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFF8012576.1"
                     /translation="MKLDLQTARRNLNSPNIKTRKRALKIIKQHKRAK"
     gene            complement(10155..10841)
                     /locus_tag="ACGEXS_001567"
     CDS             complement(10155..10841)
                     /locus_tag="ACGEXS_001567"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFF8012577.1"
                     /translation="MPKLILCRHGQSEWNAKNLFTGWEDVNLSEQGINEATRAGEKVR
                     ENNIAIDVAFTSLLTRALDTTHYILTESKQQWIPVYKSWRLNERHYGGLQGLNKDDAR
                     KEFGEEQVHIWRRSYDVKPPAETEEQREAYLADRRYNHLDKRIMPYSESLKDTLVRVI
                     PFWTDHISQYLLDGQTVLVSAHGNSIRALIKYLEDVSDEDIINYEIKTGAPLVYELTD
                     DLEVIDKYYL"
     gene            complement(11168..>11994)
                     /locus_tag="ACGEXS_001568"
     CDS             complement(11168..>11994)
                     /locus_tag="ACGEXS_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFF8012578.1"
                     /translation="YLSLIVYIILSIVKYVTGFVFNSAAVRADALNNMTDIIVSLAVI
                     IGLKISIKPADRNHPYGHLKSENISSLLVSFVIMFVGIQVVIQNAPRLFKEDDVVPNA
                     ITIIVSLISGLVMLIVFAVNQRLAKRTKSSSLNSAAKDNLSDSLVSIGTAIGLIFTQI
                     GFPIVDIILATLLGLLIVYTGFGIFKEAIFMLSDGFNETELEAYRNDILEVDEVQEVK
                     SIKGRYHGSSVFIDVTIVVDANLSLVEAHQICDNVEHHLHKKGISSVYVHPEPDHL"
BASE COUNT         3920 a         2192 c         1638 g         4244 t
ORIGIN      
        1 atcgaatcat ccctcatttt tataatatta caagattatt ataaaccgaa tgaaacaaaa
       61 aagtttttac atattttcaa caactttatt tcaataaagc atcaagttga tctaaagttt
      121 gattcatacc ttgttcaaca cccatgttta tgacttgttg agcggcttct tttgttggaa
      181 ataccgatgt tgatgtcact gtcgttttcg ttgtattgct cttggaaaat gacaaagtaa
      241 ttttcatgct tggcattttt gtatcttttg caccttgagg tgtcgcaaat gagtcaatat
      301 attcaattaa atatggacgc ttaaccgttt tatattctgc taatgtatag ctggtcattg
      361 taggtgtttt aatcgcataa aatgcatcac cgcctgaaac agcattaaag cgaaacactt
      421 tggtactagc gtcttttgga tgaaaccatt tttcaaataa tgcttttgtc gtataggcat
      481 caaacacctt ttcaatcggt gcttctatcg ttcttgaaaa aataatttta ttatcttcaa
      541 ctttaatcgt catcttccca ctccctatct tttatattta ctcatcttaa catgcattga
      601 gtaacatatg ttaccaaatc atttgttata aactatagta atattaatca ttccctctgt
      661 tgaaattgat aactacgaaa taaaaaacac actctattca gctattaaca gttgagtaga
      721 gtgtgcaaac ctgttgaatt aatgcgctaa catttcttct ttgatttggt ctttatttaa
      781 ttttgaagga taatatgttg gccagttatc catttctttt aataacgcgt cattatcatt
      841 acccataaag atatggaagt gttctgcctt tttaggtgca atgttgtgat cgctaaattg
      901 gatgaatttc ggtaagtctt tatcattacc atcgaccaat ttaaacataa atctcacgcc
      961 acggttacct ttaggatatt tcaatgtttt cttaccattg tattcatatt taccagtgtg
     1021 tttcttacca tctttagtaa atgtaatttc atttcctttt atatcaatgt tagtaatatc
     1081 agttttatat cctttgtcat aataagcttt taaatcttta gcagatttct tcggatcatt
     1141 ttcagcttta tgttccatca cttcatcaag cgtaccgtct tttaagtaag ggtaaacaga
     1201 ttgccattca ccagcataat cgcttaattc acggtcttta acttgctcat ctttaaaata
     1261 cccatcagaa attgctttgt catgtttact ttcagctttg tcgtctttca ctttaacacc
     1321 actgtctaaa gctttaccga tattttcaat attagatttc attaatgatt gatacgtaat
     1381 attatctttt ttctgttgtt ctttatttaa agactccatg ttgtagaatt ttaaaggctt
     1441 cgcatctgtt tctttacgaa ttgtttcagt cactttattc gcaacattat cttcgtaaag
     1501 aatatattta gcattgctat ctctaatttc tttaacaatt ttagttaatt ctttttgtga
     1561 tggatcttca gcattcatgt tttgaatacc tttttgaaca aaaccataac aatcagctaa
     1621 gtatccaatt gattcatgtg aaatgaatac tgcattacct tgcttatctt ttgtaacttg
     1681 cttcatatcg ttatcaattt tcttaagatc gtcgtttaat tttttgtagt ttttctcata
     1741 gtcatcttta tgttttggat cttttttcac taattcatct ttaatttctt tagcgaaagt
     1801 ttggttaatt ttaggatcta accatacgtg tggatcatat ccaccatgat gatgatgatg
     1861 ttcttctttc tcatgatcat gtccctcatg ttcatgctct tcaccatgct cgtgttgatc
     1921 agttaaaagc tttgctttat ctaatttatc ctctaaagac agttttttat ctttatcttt
     1981 gatagtagat gcaactttct tagcaaccgg atctaaatta tcccctgtat acataaacaa
     2041 gtctgacttg cttgcactta atatatcttt ttgtgttggt tcatagctat gtaaatctgt
     2101 ccctgctgga tagattgatg ataccttcac gtgttttcca ccaatttgct ctgcaaatga
     2161 ttgcaatgga taaactgtcg ttttaattgt taccttgcca tctttatctt ttccatcatc
     2221 attcccacat gcggctaatg ataaagttac ggctggtaca agaagtaaca tacctaattt
     2281 ctttttcata gtcaccctcc taaatagtaa tctttacgtt ttaaaattat atactcatgc
     2341 tacttaaaat acaagcactg tttttataaa taatataaag tcgaatcata ctttaggtat
     2401 ttaccacttt tcgttttatc taatacatga ctattgtaca aatatgatac agaaaacttt
     2461 atagtattta cgaacgtcat aatataagaa aaaaagacaa tctctatcat attgctaata
     2521 gaaattgcct tttcatattg tcctaaaata acttacccta tttttatagt gtgactttta
     2581 gatttaataa tggtatttac atgatgctat ttttatatga ttgtcatcta ctgtataaac
     2641 caatctatgt tcgtgattga tacgtctact ataataccca gtcagattcg attttaactt
     2701 ttcaggctta cctatacctt ccaatgcacc atttctgtca atacttttaa gtaactcatt
     2761 aatcttcttc accatttttt tatcgttctg ctgaaaatac ttataatctt caaaggcttg
     2821 aggcgaaaac gtaatattta acctagccat tatacatcta tatctttcgt tatagttttc
     2881 ccacgttcta gatctgcaat tgattgagct aagtgttcag cattatttgg gttctgttgg
     2941 aggtaaagtg tttccatcat ggagttataa tctgattctg acatgattac tacgttttta
     3001 tcatcagtag atgttacagt taccatatca ctatcatcat ttacttttgt cataagtgcc
     3061 tttaaattct gtcgagcgta tgaataattt ttaataatca tatttatcgc ctccttttaa
     3121 cacacctcaa ttgtacaaat atctgtacaa taaaacaagc gacacagaaa aaaactacta
     3181 atcaagattt cgcacgggga agaatcaata cctatcctgt actgattccc tactaaacaa
     3241 tgtttacatg ataatttcca cctatttaga cttcatacat taaaaagaca atctctatca
     3301 tatcgctaat agaaattgcc ttttcgtatt gtcctaataa attggtatca cttactgatc
     3361 tcaacaatta agactatttg attttatctt ttaataactt ctcataactt tcataatcat
     3421 atggatcttc aactttctcg ccattaataa atgcagttgg tgtcgttttt atatgattat
     3481 ctttcgctat ttttttatct ttctctgcag ctttccaaga cttgctatct tttgtcttgt
     3541 aatcttttat aattttattt tccgtctctt tatctaaatg cagttgttta atatgtttat
     3601 ctaatagttc ttttgttaac cattctttat tttcatcttg ctgggcagca aataattgct
     3661 tttgaaaatc taaaaatgat ttaggtgcat acatcaatac tgcatgactc gcacgcgaac
     3721 caacaattga gtctttacct aagaaagcta aattgacaaa ttggtattcc actttgtgat
     3781 tatctatata atttttacgc aactttggca tgactttttc atctaattct ttacaataag
     3841 gacatttata gtcgccatat acgacaacta atggtttgcc gtttttcgaa gatgtcgttg
     3901 ctgattcttt tttaccgcaa gctgttaaaa ttaacatgct cactataaat aatgttagta
     3961 attttttagt cataataact cctattcata tctagttcgt catattttct tcatgataat
     4021 ccgcatcatg ttgttgataa tatcgtctcg aatcaaaatc ttgcttatat gatacacgtt
     4081 tatcactaaa atcatatgca taataatgca cttcatcatc attactcaaa ggtttataag
     4141 ctaaaatgat aattttatcc ttatcataga cataaatatt agcatctttg cgttcaaaat
     4201 gatcaacacg atcaccgttg ccttttttgg caagttgttc ttgtttttga ttttgaattt
     4261 taactgcttc atctatttct ttcacatatt tatcattacc acatcctgat ataaagacta
     4321 gtaataataa taccgtcgcc acaaatcgct tcaatgctcc cactcctact atcaacttcc
     4381 aactaacttt caatataatt aatactatac accgtcaaac attaatgcgc tacaactact
     4441 accaatgcat ttaatataaa tatccaaaga aataacagtt atgtatagtc aaagaaaaat
     4501 atatttcatt ttctatgtgt atttgtaatc agtttttgat aaaataaaaa aatagcatta
     4561 ttaataaatt caaaaattat agttcaattc agagagggat gggataaaat gaactttgta
     4621 acgttgactt cggatgagtt caatgcgttt acaacaaagc atttttcaca ttacacacaa
     4681 tcagctattc attacaatca tagagttgat ttaaaaggcg atgtgcatct tgtaggggtt
     4741 aaagatgaca atggtcaagt gattgcagga tgcttattga cagaagcacg cacacttaaa
     4801 tttttcaaat atttttatac acatcgcggg ccagtgatgg attatacaaa tcaatcatta
     4861 gtagcatttt tctttaaagc attaacgtca tatttaaaga aacacaattg tttatatgtc
     4921 cttgtagatc catatttaat tgaaaattta cgcaatgcag acggtgaaat tgttaaatct
     4981 tatgataacc gagcatttgt tagaacaatg gataaattag gttataaaca ccaaggtttc
     5041 cctgtaggtt atgattcaat gagccaaatc cgttggctgt cagtgttaga tttaaaagat
     5101 aagactgaag accaactttt aaaagaaatg gattatcaaa cgagacgtaa tattaaaaaa
     5161 acatatgata ttggtgtcaa aactaaaacg ttaacgattg atgaaacgca aacttttttc
     5221 gacttattcc atatggctga ggaaaagcac ggtttcaaat tccgtgagtt accatacttt
     5281 gaagaaatgc aaaagttata cgatgaccac gccatgttaa agttggcgta tattgattta
     5341 aacgagtatt taaaaacgtt acaattaaag caacaacaat taacagctga actctcaggt
     5401 gttgaagaag cattggaaga aagtcctaat tcaaagaaaa ataaaacgaa acgcacacag
     5461 ctagaacaac agctaaatag caataagcgt aaaatcgaca atacaataga acaaattgaa
     5521 caagatggtg cagtcttaaa cttagcttct gctttattta tctacaatga gcatgaagtt
     5581 tattacttat ctagtggttc aaaccctaaa tataatgctt atatgggtgc ctaccatttg
     5641 cagtgggaaa tgattaagtt tgctaaagcg catcatattg atcgctataa tttttatggt
     5701 attaccggtg acttttcaga gtcatcagaa gactatggcg tccaacaatt caaaaaaggt
     5761 tttaacgcac atgtagaaga gtatatcggt gactttatta agcctatcaa accattacta
     5821 tataaggtac aaacgtactt aaatcataaa cgtcgttaaa atatcgctcc aagattatga
     5881 caacataaca aagaaccacg catcatgtat atggcttcat cgccatacta aaacatgata
     5941 cgtggttcta tttgattatt cttcatttat aacatttaag aaacgtctta attcttctgt
     6001 tttcggacga ttgaacatct cttctggagc cccttgttct ccgatcatgc cttcatgaat
     6061 aaatacaatg ttattagata cttctttagc aaaacgcatt tcatgtgtca caatgaccat
     6121 tgtcatgcct tcattagcca aatctttaat aacctttaaa acatcattca caagttcagg
     6181 atcaagtgca gatgttggtt catcaaacaa catcacttta gggttcattg ctagtgctct
     6241 tgcaatagca acacgttgtt gttgaccacc tgataatgca tgtggacgtt gatctttgac
     6301 atgtgttaaa ccaactttct caagtaactc aagtgatttc ccacgtgcct catccttttt
     6361 caacttttta actgtgataa gaccttccat tacattttct aatgccgtct tatgcggaaa
     6421 aaggttataa ctttgaaata ccataccaga ctgtttacga acttctattt gtgatttttt
     6481 atctttagat gtatatgttt tgccgttaac ataaactgta ccttcagttg gaatttctaa
     6541 tgcattaatc atacgtaaca atgttgtttt acctgaacca gatcgaccga ttaaggttac
     6601 aacctcacct tgttcaacag ataaatcaat acctttgatg acttcaacat cattaaatga
     6661 tttatggata ttgttcaatt gaatcatgag cgataccctc tttcaatata agattcatag
     6721 ataccttgga taattgaaat aataaagcat accacccagt acattagcgc aactaacaca
     6781 taaattgtta aatattcata tgttgtagac gcaacttctt gagcttttct aaacatttca
     6841 gccactaaaa taaatcctaa taatgatgta tctttgatta aacttaaaaa tgtattacct
     6901 agtgcaggaa tcgacactcg aattgcctgc ggtaaaatga tacgttgtat cgtttgacca
     6961 tacgtcatac cgatggaata tgcagcttct gtttgtcctt tcggtataga aataataccg
     7021 ccacgaataa tttccgaagc atacgcacct acatttaatg ataaaccaat aatagcagcc
     7081 actacaggtg ccaatgtcca ttggttgtca gcgtcatttg ttaataatct acctaattct
     7141 ggaataccat aaaatatgat aaatagttgt actatcattg gtgttcctcg aataatagat
     7201 acatagacac gtgaaatact tcttaaaatt ttactcgttg aaattcgcat taatgctgta
     7261 aataatgcaa taatcaatcc taaaacaaat gtaactaatg taataggaat tgaatatttg
     7321 accaaacctt ctagcatagg tccgaaagct tgttttgcag catctagcgc atgttgttgt
     7381 tcgctattta gatttagaaa catcttgacc aaaccatttc ttacctattt tagctaattc
     7441 accgttttcc tcgatttttt tcaagccatc attgaatttt tgaactgttt catcatctgc
     7501 ttttttagaa aatgcaaatg cagatctact ttgttcagca ttacctttga ttgctttgat
     7561 cttagcatta ggtttttgtt ttttataatc caagtatgac agactatcat taaatgtacc
     7621 atcaacacgc ttagacaata ataaatccat tgattggtta aagccatcaa cttttgtaat
     7681 atcagcacct ttatccttag ctaatttacc ataattagat gtgaatgttt gtgctaactt
     7741 cttaccttta acatcatcaa aatctttaat atctttttca ttttcacgaa taactaaaac
     7801 agcacttgag aatgtgtaag gcttagaaaa tttatatttc ttttctctat caggattaat
     7861 acctacttgg ttcgcgatta catcaaaacg ccctgcgtct aaacctgcaa acatagaatc
     7921 ccaagaagtt tcattaaatt taagttttaa accttcttct ttagccactg ctttaataac
     7981 atcaatatcg taaccagtta atttaccatc tttatcgtgg aaactaaatg gtgcataagt
     8041 cccctcagtc ccaacattaa ttgtcttgct atctttacta gcttccttgt ctttcgacga
     8101 attgtttccg catgcagcca atatgaaaac gaaagctatc atcacaaata aaagtctttt
     8161 catagaatat atctccttat tcttattatt ctaatcggaa taaaaatatt ttaatctgaa
     8221 atgccggata agtcaatatg ttgaactttt gctaaaaagc tttatatagc tagcaactta
     8281 tatttattga cgagaatcaa cttctttttt cttaccttgt aacataaatg aacaaaagaa
     8341 actaacaact gtaatcaata atgcaaataa aaatccagcg tgataaccat gcaacatcgt
     8401 atcaacttta atttgattca tcatatcaat tttgttcatg ccattatagt ctgacatagt
     8461 tggagaaaag tttttgcttg catgggataa aatagtaatt aatgcagctg ttccgataga
     8521 accagcaatt tgttgtactg tgtttgtcat agatgaacca tgtgcattca tttctggctt
     8581 taattgattc atcgtatggg tcattactgg aattaatcct aacgcgatac caaccattcg
     8641 aatcgcataa actgttgcca acatgattgt tgatgtttgt tcatccatta caacaaaata
     8701 agccgtagta ataacaacaa tcgccatacc tactaaagca agatttctag gaccaaactt
     8761 ttcatataaa gcacccgaag tcatcgacat aaatgccata acaattgctc ctggtaataa
     8821 agttaatcca gaatctaaag ctgaacgatg caataaattt tgcacaaaga taggtaaaat
     8881 cgtttccgaa ccaatcatcg ataccattgt gacacccata ataatgatac caactgtaaa
     8941 ttgtttattt ttaaatacag caaaatttaa taatggtgtt gatagccgat attgacgtaa
     9001 tacaaataat gcgactaaaa caacgcctcc aatcatagat actaatacga taggattatc
     9061 aaaacctttt tctgaaattg aactcgttcc atacaataac ccgccgaaac ctagtacaga
     9121 aaaaatgaca gaaataaaat caataggtac tttaattgta gttccaacat tttttatcgt
     9181 tttaaatcca attaaaaatg tcacagctgc aattggagcg acaacatgga aaagcgatct
     9241 ccagttaaaa tattcaacaa ggtaacctga aagagtaggt ccaatagctg gtgctaaacc
     9301 aattaccaaa ccaaatatcc ccatatattt accgcgttca tgtggttgga aaacatccaa
     9361 aatcgttgtc atcatcaatg gcatcataat acctgcgcct aacgcctgaa taattctagc
     9421 aactaataat gttgcaaagt gcggactcaa gccgccgagt aatgtaccta agaaaaagat
     9481 agaaataccc actaaaaaca cttgtcttgt tgtataacgt tgtataacaa gcgcggacaa
     9541 aggaataaca atcccattag tcaataaaaa agctgttgtc agccattgaa cttgtgtata
     9601 tgaaatttca aaatctttca taatacttgg taaagccgtc actaatagtg tttcatttaa
     9661 taatccaaaa aatccaccca tcagcatagt aataatcatc gttaattttt gtttcttcga
     9721 catttaaagc ttccccctca ccatctaaaa aacgacacac atttttagat atccggtaat
     9781 tatacccatt ttccaaaaaa ttagcacgac tttattacaa tttctttaaa acgtaatcat
     9841 tactatttac atatgagtag ttatcggtca taatatattt acaaggaggg atagagatga
     9901 aattagattt acagactgct cgtcgtaatt taaatagccc taacattaaa acaagaaagc
     9961 gtgctttaaa gattattaag caacataaaa gagcgaaata attgttttaa tttgactata
    10021 ttgtatgtct aactatattt tcgagtatcc ctagctcgtt aatataaact ctatacttaa
    10081 ctatttcctt acattacttc acatatttaa gagctgcata tacactcact ccttgtgtac
    10141 atgcagctct ttttttataa gtagtattta tctataactt ctaaatcatc cgttaattca
    10201 taaacaagcg gtgcacctgt tttaatttca taattaatga tatcttcatc tgacacatct
    10261 tcaagatatt taatcaatgc gcgaattgaa tttccgtgtg cagaaactaa taccgtttga
    10321 ccatctagca aatattgtga aatatgatct gtccaaaatg gtatcactcg aactaaagta
    10381 tctttcagac tttcagaata aggcataata cgtttatcta aatgattata tcgacgatca
    10441 gctaagtaag cttcacgttg ttcttcggtt tcagcaggtg gtttcacatc ataagaacga
    10501 cgccaaatat gtacttgttc ttctccaaat tcttttctag catcatcttt atttaagcct
    10561 tgcaatccac catagtggcg ttcatttaaa cgccagcttt tatatacagg aatccattgt
    10621 tgtttagatt cagttaaaat ataatgcgtt gtatctaaag cacgtgttaa taacgatgta
    10681 aaagctacat cgatggcaat gttattttct cttacttttt cacctgctct agtcgcttca
    10741 ttaatacctt gttcagataa attaacatct tcccatccag taaataagtt tttagcattc
    10801 cactcgcttt gtccatgacg acataaaatt aattttggca taacttataa cctctttcct
    10861 ttaatcttta ggacgttatt tcagggatat tttaagatga tttaatctta gtaattttct
    10921 tttcaatata ttttaaaatt caatgattaa atcaattatc actagacatt aaatacataa
    10981 atcctattcc ccattttcat tttttaattc ataaatgaat caataaccac ctaataacaa
    11041 atcatattat acacctttgt tcgctatttt tctaaggttt aaaaaatatt tttaggtaaa
    11101 cctaaaaata gatgtaataa aaacgcctcc tcagatattt atatatctat gaagacgttt
    11161 aaatacatta tagatggtct ggttctgggt gaacgtatac tgaggaaata ccttttttgt
    11221 gcaaatgatg ttcgacattg tcacaaattt gatgcgcttc tactaaggaa aggttagcat
    11281 ctacaacaat tgtgacatca ataaacacac tacttccatg gtaacgccct ttaatacttt
    11341 taacttcttg tacttcatca acttctaaaa tatcattgcg atacgcttct aattcagttt
    11401 cattgaaacc atcactcaac ataaaaattg cttctttaaa aataccaaaa ccagtataaa
    11461 cgattagtaa gcctagtaat gttgctaaaa taatatcgac aattgggaaa ccgatttgcg
    11521 taaaaattaa tcctatcgct gttccaatgc tgactaaact atccgataaa ttatctttgg
    11581 cagccgaatt taaagaacta cttttcgttc ttttcgctag tctttgattg actgcaaata
    11641 caatcaacat tacaagacca ctgattaagc tgacgataat tgttattgcg ttaggtacaa
    11701 cgtcatcttc tttgaacaaa cgaggtgcat tttgaataac tacttggata cctacaaaca
    11761 taatgacaaa tgacaccaat aatgaagaaa tattttcaga cttcaaatgg ccataaggat
    11821 gatttcgatc ggcaggttta attgaaattt tcaatccaat aataacagct aaagaaacga
    11881 taatatctgt catattgttt aatgcatcgg ctcttacagc tgcagagtta aagacaaaac
    11941 ccgtgacata cttaacaata gataagatta tatatacaat taaactcaaa taag
//
LOCUS       DAXWFK010000077        11636 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.077, whole genome shotgun sequence.
ACCESSION   DAXWFK010000077 DAXWFK010000000
VERSION     DAXWFK010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11636
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.077"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(203..1561)
                     /locus_tag="ACGEXS_001569"
     CDS             complement(203..1561)
                     /locus_tag="ACGEXS_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate:H+ symporter"
                     /protein_id="HFF8012579.1"
                     /translation="MFNEIWPLISVVLGIVILLVLIIGFKLNTFISLIITSMITALML
                     GIPLTKIMETIEKGMGSTLGHIALIFGLGAILGKLLADGGGATRIADTLIQKFGQKHV
                     QWAMLVAAFIVGIALFFEVGLVLLIPLVFTVAKRANVSVLKLGLPMVTALSVTHGFLP
                     PHPGPVVIAKELKANVGDVLLYGMIIAIPVTLIAGPIFNKVAQKMIPSAYTREGDISA
                     LGAQKEFTDQEMPGFGMSLLTATLPVILMLVSTITQLVTGHDKPTNLFESIIYMIGTA
                     GTAMLIAVLFAIVTMGLMRKRKMNHIMESVTNAIYPIGMMLLIIGGGGTFKQVLIDGG
                     VGNTIAKMFEGTEMSPILLAWIVAAVLRIALGSATVAAISTTGIVLPLLQSSDVNVAL
                     VVLAIGAGSVILSHVNDAGFWMFKEYFGLTVKETFLTWSLLETIISVSGIIFILFISL
                     FV"
     gene            complement(1678..3231)
                     /gene="gntK"
                     /locus_tag="ACGEXS_001570"
     CDS             complement(1678..3231)
                     /gene="gntK"
                     /locus_tag="ACGEXS_001570"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA];
                     GO_process: GO:0019521 - D-gluconate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFF8012580.1"
                     /translation="MKYMIGVDIGTTSTKSVLYDENGTFIMKHQIGYDLHTPNVDVSE
                     ENPDELFDAVLMTIKYVMRESKVNKDDIKFVSFSAQMHSLIAMDQQHQRLTNNITWAD
                     NRAAKYATVINEVHDGNAIYQRTGTPIHPMSPLAKIFWMKHEWQDVFQRTAKFADIKT
                     YIFYHLFDTYIIDYSMASATGMFNLETLDWDVEALELLGISKEMLPELVPTTYVMKGM
                     KERYATLMGLNKDTPFVIGASDGVLSNLGVNSVGKGEVAVTIGTSGAIRTVIDKPRTD
                     YKGRIFCYVLTEDHYVIGGPVNNGGVVLRWLRDELLASEVETAKRLGVDPYDVLTQIA
                     KRVKPGADGLIFHPYLAGERAPLWNANARGSFFGLTLSHKKEHMIRAALEGVLYNLYT
                     VYLALIEVMNETPKMIKATGGFAKSEVWRQMMSDIFDTELVVPESYESSCLGACVLGL
                     KAVGDIEDFSIVSSMVGATNNHTPIEENVTVYQEIVSIFINLSRSLTENYEQIADFQR
                     QHIAENKTQ"
     gene            complement(3255..3935)
                     /locus_tag="ACGEXS_001571"
     CDS             complement(3255..3935)
                     /locus_tag="ACGEXS_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8012581.1"
                     /translation="MMYGYPEKWLEGMTTGEGIAAELRLGIVNGHIAEGTLLTENQMA
                     KQFNVSRSPIRDAFKLLQQNQLIQLERMGAHVLPFGEQEKKGMYDLRLMLESFAFSRV
                     KNQERLPIVKEMKKQLEMMKVAVKFEDAESFTKHDFEFHETLIKASNHQYLNSFWSHL
                     KPVMMALVLTSMRQRMQQNPQDFERIHHNHQVFIDAVEQYDSQILKEAFHLNFDDVGK
                     DIEGFWLN"
     gene            complement(4089..4853)
                     /locus_tag="ACGEXS_001572"
     CDS             complement(4089..4853)
                     /locus_tag="ACGEXS_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFF8012582.1"
                     /translation="MSNYSTGELAKLCNVTTRTIQYYDRKGILKPQGFTEGKRRVYTE
                     QQRQTLELILLLKDLGCALSDIDMLLKGEGTLKTLNTLLTMKQQEINQQVKQQQAVLN
                     KIKNVQYYVNEASTSPITHLKDIEHVMSKSAEMKSIRRNIWISAGIIGIIQYSSIISA
                     ILMKNKWPFLIALPFMIGYGIGVTFYYQQKVAYLCPNCQHIFSPSLWAVIKAKHTATT
                     RRFECPNCHETHYCIEVPKAHMSTEQLEISHIQHNN"
     gene            complement(5018..5710)
                     /locus_tag="ACGEXS_001573"
     CDS             complement(5018..5710)
                     /locus_tag="ACGEXS_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFF8012583.1"
                     /translation="MYVDRKPSLYLEDLRHDFKNSLSKFENGDEAFDTLLGFVELDHI
                     YSSALKEISTKLSILDDNFNHIYKHNPIHHMERRVKEMRSLIEKLNRKGLQISAETAK
                     EHILDIAGIRVVCNYLDDIYLIEEMLLKQEDVQLIKRKDYIQHPKENGYRSLHIVVSI
                     PVFLAERVEVLPVEIQIRTIGMDMWASLEHKIRYKNNAETEKYRDLLKECATEITEVE
                     DKLQQIHSEITE"
     gene            complement(5941..6357)
                     /locus_tag="ACGEXS_001574"
     CDS             complement(5941..6357)
                     /locus_tag="ACGEXS_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFF8012584.1"
                     /translation="MPFTMKGYPEKWKEFEVIKRQKAIEIANAMLKEGYLEKDVVPIA
                     AKKAKDWYRALSKEEIKALEEENFVQFHTATMEKTEVDSEYATTDNEEHIQEQSNQDK
                     SYFDSESIGNQSGGVRQREPLMDGPHPKVVESFKDV"
     gene            complement(6496..8319)
                     /locus_tag="ACGEXS_001575"
     CDS             complement(6496..8319)
                     /locus_tag="ACGEXS_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFF8012585.1"
                     /translation="MIIPAIIGYLCGNFQFGLLVATGTLAHIYVFKGPSRSKLRTVII
                     CNLAFAICMMLGTLTAKTPLVFGMTLLIVTVIPFYIFTALKIAGPSSTFFIVTFSLPI
                     NLPIAPEEALYRGFAILVGGILATMMVLITIVFSKNKAEEQAIQNDFKLISKLLHTYN
                     DKSAFLQVAKTAVDSFKASDKLLITSTSSNDKLSRRFQKLLLLHTSAQGIYSELLELN
                     AKQIRPLPDELIEMMDHIIAQLDNSEENVRYWRKEVTVTEEFQNLFNHILKIDEMVHA
                     NEARIAYEADMRKPLYSKRIYQNLTLDSIVFRNTLRYTAIMMIAIFIALMFDFEKAYW
                     IPLSAHTILLGTSTIHAIERGMARGLGTILGVLVLSVILLFSIPTPVAVILMGIAALF
                     TEALVGANYAIAVVFITIQVILMNGLASQNLTINIAFPRVIDVAMGIVIAIIGLFVLG
                     QRTASALLPNVMAEVVRKEATLFHYLFSENQYKDDVYQKNTAMNLSVKLNNMTQVYNA
                     ANGELFSDKTLIQNYYPSLFALEEISFMLNRAMANEDRLTINEQLMGEYLAIFENIAK
                     HFELNTELEIKILPDLPQYNYIQSAMMNIQHNGFRERDKNV"
     gene            complement(8797..10074)
                     /locus_tag="ACGEXS_001576"
     CDS             complement(8797..10074)
                     /locus_tag="ACGEXS_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8012586.1"
                     /translation="MKEKRTNVRWMFALAFFFIGVIAYMDRANISYIAKQMMDDLGMT
                     KPQFGLLASFFSLGYALMQVPSGMLAEKFGPRKMITIALVWWSAFTILTGMIKNHGLI
                     YLVRFLFGVGEAPMYPSNAVFNSFWFSKNEKGRASSALLAGSYFGPVLAPIVTIAIVN
                     AFNWQAVFYIFGAVGILMAVLWAIIAKDLPEQHRMVNEAEKRFIMENRDIVATEKSSP
                     PWNDFFKRFSFYAIAIQYFVVQFIITLFLIWLPTYLTEVFHVNFKEMSISSLPWLLMF
                     FLILSAGAISDRVLGLGRSKFVARGVIAIAGFIVFAVSIIFAVRTGNLYVSIFWLSLG
                     LGGIGISMGMSWAAATDLGRNFSGTVSGWMNLWGNIGALISPLLAGLFVEHLGWTMTF
                     QLLIVPAVIAVIMWFYVKPDQPLIVSDDKAIEK"
     gene            10621..11232
                     /locus_tag="ACGEXS_001577"
     CDS             10621..11232
                     /locus_tag="ACGEXS_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFF8012587.1"
                     /translation="MEQIITEFISRFGYAAIFILILLENVLPIVPSEIILTFAGLMSV
                     KSHLSILTLFIIATIASFIGLLILYYICRLISEERLYRFIDRHGKWIKLKSKDLKRAN
                     DWFKKYGVWAVFICRFIPVLRVLITIPAGVNRMNVVTFTVISLIGTTIWNFGLILLGR
                     TLSDSFGMLMTGLHTYSRIMYVVIIIAVIYFAIRYIGKRKRVK"
     gene            complement(11241..11339)
                     /locus_tag="ACGEXS_001578"
     CDS             complement(11241..11339)
                     /locus_tag="ACGEXS_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012588.1"
                     /translation="MPNKLKKAVNHFNRWNDSLLFVYRNLKFMPLC"
     gene            complement(11329..>11636)
                     /locus_tag="ACGEXS_001579"
     CDS             complement(11329..>11636)
                     /locus_tag="ACGEXS_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8012589.1"
                     /translation="FGWLNESNVIILSVIIFILGTCVGIVPALLSTIISKRFEHIKGK
                     VLGVFNFVRYIGMTVGALLIGIISQPLVAFYFTTITIMLIVIFLYIKIVDFQLKYAK"
BASE COUNT         3693 a         2248 c         1686 g         4009 t
ORIGIN      
        1 atatactaaa catttcataa ttatcgcctg tacaatacgc acaaaaacat gtcttgaaac
       61 gcctttcatt actctaaaat acccaatata ctttttatat cgttcggatt ctgagtattt
      121 cagacgattt tctgtataaa aataaacgtg tttcaaggca atatattgca attacctaaa
      181 aacacgttta cttaatattt agttaaacaa ataagctaat gaataaaatg aagatgatac
      241 ctgaaacgga aataatcgtt tctaataatg accatgttaa gaatgtttct tttacagtta
      301 aaccaaaata ttctttaaac atccaaaatc ctgcgtcatt tacatgagac aaaatcacac
      361 tacctgcacc tatcgcaagt acaactaatg caacatttac atctgatgat tgtaataatg
      421 gtaagacaat acctgtagtt gaaatcgcag ctactgtagc cgaacctaat gcgatacgta
      481 gcacagctgc aacaatccat gctagtaaaa tcggagacat ctctgtacct tcaaacattt
      541 tagcaattgt atttccgaca ccgccgtcaa ttaatacttg tttaaatgta ccgccaccgc
      601 caataatcaa taacatcatt ccgattggat aaatcgcatt cgtcactgat tccataatat
      661 gattcatctt acgctttctc attaatccca tcgtaacgat tgcaaataat actgctatta
      721 gcatggctgt ccctgctgtt cctatcatat aaatgataga ttcaaataga tttgtaggtt
      781 tgtcatgccc agttacaagt tgcgttatcg tagacactaa cattaatatg actggtaatg
      841 ttgctgttaa taaactcata ccaaatcctg gcatctcttg atccgtaaat tctttttgtg
      901 cacctaacgc tgaaatatcg ccttctcgtg tatacgcaga cggaatcatt ttttgtgcaa
      961 ctttgttaaa tataggccct gcaatgagtg taactggaat ggcaataatc ataccataca
     1021 gtaatacatc tccaacattt gcctttaatt cttttgcgat gactaccggt cctggatgtg
     1081 gtggtaaaaa gccatgtgtc actgataaag ctgttaccat aggtagtcct agttttaaca
     1141 ctgaaacatt tgcgcgtttt gctactgtaa atactaatgg aatcagtaag actaaaccta
     1201 cttcaaagaa caatgcaata ccgacgataa atgctgcaac aagcattgcc cattgtacat
     1261 gtttttgacc aaatttttga atcaacgtgt ctgcgattcg agttgcacca ccaccatcag
     1321 caagcaattt cccaagtatg gcacctaaac cgaatatcag tgcaatgtgg ccgagcgtac
     1381 tgcccattcc tttctcaatc gtctccataa ttttagtcaa tggtatacct agcattaacg
     1441 ctgtaatcat cgatgtgata attaatgaaa taaatgtatt taatttaaac ccaataatta
     1501 atactaataa aataacgata cctaaaacaa cactgattaa cggccatatt tcgttaaaca
     1561 tgacattccc ctctttctct tttcaataga atgtaacacc gtcgtcgcgc acgtattcca
     1621 cataatatat agtagtcatg gcacaacaac tttatctaag atgcttgagt gacgtattta
     1681 ttgtgtttta ttttcagcga tatgttggcg ttgaaaatct gcaatttgtt cataattctc
     1741 tgttaaagaa cgacttaaat tgataaaaat ggatacgatc tcttggtaaa cagtgacatt
     1801 ttcttcaatc ggcgtatgat tgtttgtggc accgaccatc gatgaaacga ttgaaaaatc
     1861 ttcaatgtca cctacagctt taagtccgag cacgcaggca cctaagcatg aactttcata
     1921 actttcagga accactaact ctgtgtcaaa tatatctgac atcatttgac gccatacttc
     1981 acttttcgca aaaccacctg ttgcttttat catcttaggt gtttcattca ttacttcaat
     2041 aagcgcaaga tagacggtat acaaattgta aagaacacct tctaatgcag cgcgaatcat
     2101 atgttctttt ttatgagata aagttaaacc gaagaatgaa cctcttgcat ttgcgttcca
     2161 aagcggcgca cgttctcctg ctaaataggg atggaatatt aaaccatctg cacctggttt
     2221 aacacgcttt gcaatttgag ttaagacatc ataaggatca acaccgagac gtttcgcagt
     2281 ttcgacttca ctcgctagca actcgtcgcg caaccatctc aatacgacac caccattatt
     2341 tacaggacct ccgatgacgt agtggtcctc tgttaagaca taacaaaata ttctaccttt
     2401 gtaatcagta cgcggtttat ctatcacagt acgaatcgcc ccagatgtac cgattgtgac
     2461 agcaacttct cctttaccaa cactattgac acctaaatta gaaaggaccc catcactcgc
     2521 accaataaca aacggtgtat ctttattaag ccccattaat gttgcataac gttctttcat
     2581 acctttcatc acatacgttg ttggaactaa ttccggcaac atttccttgg aaatacccag
     2641 cagttctaat gcctcaacat cccaatctaa tgtttctaaa ttaaacatcc ctgttgcgga
     2701 agccattgaa taatcaatga tatatgtatc aaataaatga tagaaaatgt atgttttaat
     2761 atctgcaaac ttagcagtac gttgaaatac atcttgccat tcatgtttca tccaaaaaat
     2821 cttcgctaat ggcgacatag gatgaatcgg tgtgcctgtt cgctggtaaa tcgcattgcc
     2881 atcatgcact tcatttatta ctgttgcata ttttgcagcg cggttatctg cccaagtaat
     2941 attatttgtt aatctttgat gttgctgatc catcgcaatc aagctatgca tttgcgcact
     3001 aaatgacaca aacttaatgt cgtctttatt aactttggat tctctcataa catatttaat
     3061 agtcattagt actgcatcaa ataattcatc tgggttttct tctgagacat caacgtttgg
     3121 tgtgtgtaaa tcatagccta tttgatgttt catgataaaa gttccatttt catcatataa
     3181 gactgacttg gtactcgtcg ttccaatgtc gacaccaatc atatatttca tgataaatcc
     3241 ttctttcttt cattttaatt caaccaaaat ccttcaatat ctttaccaac atcgtcgaaa
     3301 tttaaatgaa acgcttcttt caaaatttga ctgtcgtatt gttccactgc atcaataaac
     3361 acttgatgat tatgatgtat gcgttcaaaa tcttgcgggt tctgttgcat acgttgtcgc
     3421 atggatgtta aaacgagtgc catcattact ggttttaaat gactccaaaa tgagtttaaa
     3481 tattgatgat tagatgcttt gattaatgtt tcatgaaatt caaagtcatg cttcgtaaat
     3541 gattctgcat cctcaaattt tactgccact ttcatcattt caagttgttt cttcatttct
     3601 tttacgatag gtagtcgctc ttgattttta actcttgaaa atgcaaatga ctctaacatc
     3661 agtcgcaaat catacattcc tttcttttct tgttccccaa acggcaacac atgtgcaccc
     3721 attctttcta attggatgag ttgattttgt tgcaataatt taaatgcatc tcgaattggc
     3781 gaacgactca cattaaattg ctttgccatt tgattttcag tgagtaacgt accttcagct
     3841 atgtgaccat tcacaatgcc taagcgtaat tctgccgcga taccttctcc agttgtcata
     3901 ccttccaacc atttctctgg atatccatac atcatcaaag tcactccttc attacacgac
     3961 atacttgtat acaagtatgt taatatagtt attatgagtt tgcaagcgct ttctttacga
     4021 gcactaaaat agtgaccacc ccttttcgat ttaaatttaa aggaaatggt cactatcaca
     4081 cgaatgattt aattgttatg ttgtatgtgg gatatttcta attgttctgt actcatatgc
     4141 gctttaggta cttcaatgca ataatgcgtt tcatgacagt ttggacattc gaatcgacgt
     4201 gttgtcgctg tatgtttcgc tttgataact gcccacaaag atggtgagaa tatatgctgg
     4261 cagttaggac ataaataggc aaccttttgt tggtaataaa aagtaacacc aatgccataa
     4321 ccaatcataa atggtaaagc aattaaaaac ggccatttat ttttcatcaa aattgcactt
     4381 ataatgctag aatattgaat tattcctata ataccagcac taatccaaat gttacgacga
     4441 atacttttca tttcagctga tttactcatg acatgctcta tgtcttttaa gtgtgtgatt
     4501 ggagacgtcg acgcttcatt tacgtaatat tgaacatttt taattttgtt taataccgct
     4561 tgttgctgtt taacttgttg gttaatttct tgttgtttca tagttagtaa agtattgagc
     4621 gtcttcaaag taccttcacc ttttagcaac atatctatat cgcttaacgc acaacctaaa
     4681 tctttaagca ataagattaa ctctaatgtt tgtcgctgtt gttctgtata cacacgacgc
     4741 tttccttctg taaatccttg tggtttcaaa atacctttgc gatcataata ttgaatcgtt
     4801 cgtgttgtca cattgcataa ttttgcgagt tctccagtcg aatagttaga catagattcc
     4861 acctcctata attaccatag ttgatgaccc gacgtcacga gcaagtacaa tttccacatt
     4921 ttaaagaaat ttattatact aggcgtctta tttttatgat ttcgtaccat gttgatttac
     4981 aaactcactc aaactaagta acacacctac taaacatcta ctctgttatt tcagaatgaa
     5041 tttgttgtaa tttatcttca acttcagtaa tctctgtcgc acattctttc agtaaatctc
     5101 gatacttttc cgtctctgca ttgtttttat aacgtatttt atgttctaaa cttgcccaca
     5161 tatccatacc tatcgttcta atttgaattt caacaggcaa tacctcaacg cgttctgcta
     5221 aaaagactgg aatagataca acgatatgta aactgcggta accattttct ttagggtgct
     5281 gaatataatc tttacgtttt atcaattgta cgtcttcttg tttaagcaac atctcttcaa
     5341 tcaaataaat atcatctaag taattacata ctacgcgaat tccggcaata tccagtatat
     5401 gttctttggc agtttctgcg ctaatctgta atcctttacg attaagcttt tctattaaac
     5461 tacgcatttc tttcacacgt cgctccatat gatgtatagg attgtgttta taaatgtgat
     5521 tgaaattgtc atctaaaata ctcagtttag tgcttatttc ctttagtgct gacgaataaa
     5581 tatgatctaa ctctacgaaa cctaataacg tatcaaatgc ttcatcacca ttttcaaatt
     5641 tacttaaact atttttaaaa tcatgtcgca aatcctctaa atatagtgat ggttttcgat
     5701 ctacatacat ttttatacta acctccgata tataatcatc tttattgtac ctaacatttt
     5761 attaagataa tactaataca ctatcgaact ttgggctgat actgaaacag cacaaagaaa
     5821 ctcaaacaat tcgaattatg tatcaaaacc ttcaattaca ggaagtgatt ttaaaatgat
     5881 ttaacgcaaa aaaacacctg ttaccgttat ataggtacaa gtgcttaatt gatagagatg
     5941 ttatacgtct ttaaatgatt ccacaacttt tggatgtgga ccatccataa gcggttctct
     6001 ttgtcgaacg ccaccacttt ggttgccaat tgattcgcta tcaaagtacg atttatcttg
     6061 atttgattgt tcttgaatat gttcttcatt atcagttgtt gcatattcac tatccacctc
     6121 tgttttttcc attgttgctg tatggaattg tacgaagttc tcttcttcta atgctttgat
     6181 ttcttctttc gataacgctc gataccaatc tttcgctttt ttcgccgcaa taggaacaac
     6241 atctttttca agatatcctt cttttaacat tgcattcgca atttctatcg ctttttgccg
     6301 tttaattact tcaaattctt tccatttttc ggggtaacct ttcattgtaa agggcatttc
     6361 cttaacctcc aatatgttat aattcatgtt atataccacc ataattataa aacgaaacat
     6421 attaaacaca attttttaga ctcccgtcat aataacttca gacaaacgtt agtctgacct
     6481 gataaatatt taattttaaa cgttcttgtc tctctcacga aaaccattgt gttgaatatt
     6541 catcattgct gattgaatat aattatactg tggtaaatct ggtaatatct ttatttctaa
     6601 ttctgtattt aattcgaaat gcttagcgat gttttcaaat attgctaaat actcgcccat
     6661 taattgttca tttattgtaa gtctatcttc attagccatg gctctattta acataaagct
     6721 aatttcttct aatgcgaata agctaggata ataattttga atcaatgtct tatcactaaa
     6781 taattcacca ttagctgcat tataaacttg cgtcatgtta tttaatttca cagataggtt
     6841 catagccgtg tttttctgat acacatcatc tttatattga ttttcagaaa ataaataatg
     6901 aaagagcgtt gcttctttac gaacaacttc agccattaca ttaggaagca atgcggatgc
     6961 ggtacgttgt ccaaggacaa ataaacctat gattgcaatc acaataccca ttgcaacgtc
     7021 aataactctt ggaaacgcaa tgttaattgt taaattctgt gatgctaatc cgttcattaa
     7081 aataacttgt attgtaataa aaactactgc aatcgcataa tttgctccca ccaatgcttc
     7141 agtaaacaat gctgcaatgc ccattaaaat tactgcaaca ggtgttggta ttgaaaacaa
     7201 caatatgact gaaagtacta acacacctaa aatagtacct aaacctcgtg ccatacctct
     7261 ctcgattgca tgtatagttg atgttcctag taatattgta tgtgcagata acggtatcca
     7321 gtatgctttt tcaaaatcaa acattaacgc aataaatatc gctatcatca taatcgctgt
     7381 atatctcaat gtatttctaa aaacaataga gtctaatgtt aaattttgat aaatgcgttt
     7441 actatataaa ggttttcgca tgtctgcttc atacgcaata cgcgcttcat ttgcatgcac
     7501 catttcatca attttcaata tatggttgaa taaattttga aattcctctg ttactgtcac
     7561 ttcttttcgc caatatctta cattttcctc actattatct agttgtgcaa tgatatgatc
     7621 catcatttca attaactcat ctggcaatgg tcgaatttgt ttagcgttca actctaacag
     7681 ttcagaataa atcccttggg cagatgtgtg taataataat aatttttgga aacgtctact
     7741 taatttatcg ttacttgaag tagaagtgat taataattta tcagatgctt taaaactatc
     7801 caccgctgtt tttgctactt gtaaaaaagc agatttatca ttataagtgt gtaacaactt
     7861 agatatgagt ttaaaatcat tttgaattgc ttgttcttca gctttgtttt tagaaaatac
     7921 gatcgtgatt aacaccatca tagtggcaag tataccgcct actaaaatcg caaagcctct
     7981 atataatgct tcttcgggag ctataggtaa gttaatgggt agactgaatg tcacaatgaa
     8041 gaatgtcgat gacggtccag ctatttttaa ggcagtaaat atataaaatg gtataaccgt
     8101 aacaattaat aatgtcattc caaaaacgag tggcgttttg gctgttagcg taccaagcat
     8161 catacatatt gcaaacgcta aattacaaat tattacagtt cgcagcttag atcgcgacgg
     8221 acctttaaaa acataaatat gggctagtgt tccggttgca actaataatc caaattggaa
     8281 attaccacat aagtaaccga ttattgcagg aataatcatt aacagtccct gtcttacacc
     8341 tttataaaag tctaagttaa gtcgattgat ttttatgagt gatgtcaaaa agatgtcatt
     8401 tcgacttctc ctttccttgt caaaatattt ccataatctt tttattagca tagttaaaat
     8461 gaatttgttg ctataaattt taattacgca acatatgtca ttataacacc ttatgctcaa
     8521 ttatctaaat ttaaaaactt ttaaagtcca attaaaatta atttctaatg ttatttaaca
     8581 tcgttgaacc tttggtcgcc cacctttgcg ctagttaaaa tttatatgta cttttacaag
     8641 cgttacctga ctaatgtttt aaaaccttat ttctttacat atatcatttc aataacttca
     8701 ttttcatctt aaaccatatc tgttttaaaa agtttcaatt tcaacgcaaa aaaagcaata
     8761 agccccaact atgtgaaagc ttactgcttg tttaaattat ttttctattg ctttatcatc
     8821 actaacaatt aaaggttgat ctggtttcac atagaaccac ataatcacag cgattactgc
     8881 tggaacgatt aacaattgga atgtcattgt ccaacccaaa tgttctacga atagacctgc
     8941 taatagcgga ctgattaatg cacctatatt accccataag ttcatccacc ctgatactgt
     9001 accagagaag ttacgtccta agtcagttgc tgcagcccaa ctcataccca ttgaaatacc
     9061 gataccacca agacctagtg ataaccagaa aatacttaca tataaatttc ctgtgcgtac
     9121 agcaaagata attgaaactg caaacacaat aaatcctgca attgcaatta cacctctagc
     9181 tacgaatttt gaacgaccta atcctaatac acggtcagaa attgcacctg ctgataagat
     9241 taagaagaac attaataacc aaggtaatga actaatgctc atttctttaa agttaacgtg
     9301 gaatacttct gttaaatacg tcggtaacca aattaagaat aatgtaatga taaattgtac
     9361 aacaaagtat tgaattgcaa ttgcatagaa gctaaaacgt ttaaagaaat cattccatgg
     9421 tggtgatgac ttttcagtag ctacgatatc acgattttcc ataatgaaac gtttctccgc
     9481 ttcattaacc attctatgtt gctcaggtaa gtctttggca ataatcgccc ataatacagc
     9541 cattaaaata cctactgcac caaaaatgta aaatactgct tgccagttaa atgcgttaac
     9601 aatagcaatt gtaactattg gtgctaatac aggtccgaaa tatgatcctg ctaataatgc
     9661 acttgatgct ctaccttttt catttttaga gaaccaaaat gaattaaaca cagcattaga
     9721 agggtacatt ggcgcctcac ctacaccaaa taagaatctc actaaataaa ttaaaccgtg
     9781 gttcttaatc atacccgtta agattgtaaa tgcactccac caaactaatg caattgtaat
     9841 catcttacgt ggaccgaatt tttcagccaa catccccgat ggtacttgca ttaatgcata
     9901 acctagagag aagaatgatg ccaataaacc aaattgtggt tttgtcatcc ctaaatcatc
     9961 catcatttgc ttagcgatat atgaaatgtt tgctctatcc atatacgcaa taaccccaat
    10021 aaagaagaac gcaagcgcga acatccacct tacgttagtt cgtttttcct tcataactat
    10081 tcctacttcc ctaattaatt ttttaaaact taccctgaaa cgttaaaatt ttcggacatc
    10141 tggcatgtcc acccctactt tgtcacaaaa ttgtaccaaa gttatttttt ataatattat
    10201 cagacatgga aagcgtttac aatagaatct ataaaaattt tattaaattc cgcttaactt
    10261 ttgctattaa aacgatataa aggaactttt agttacatag tagcctatcg tttgttaatt
    10321 aaaaatactg caatcctagt gttttaaaac gtatattgca ggctttctta catattcgac
    10381 attcatttta aattgcatat aactacatta accattcatt gtgcagcgcc tgttgtttag
    10441 atgatcattt attgactttt tgctcttata gcaagcaatt ttttgattta ttgcttataa
    10501 tacattttaa gacaaatgtt tgtttcaaca tcgcatgcga catcattaat tattcatttg
    10561 aatttatcca gtttctattt gtctgcaatt gctgaaaatt aaaggagctt atttttaata
    10621 atggaacaaa ttatcactga atttattagc cgttttgggt atgcagccat ttttatatta
    10681 attttattag aaaatgtact acctatcgta ccatcggaaa ttattctgac atttgctggt
    10741 cttatgtctg ttaaatcaca tttatcaatt ttaactttat ttattattgc aactatcgca
    10801 tcgtttatag ggctgttaat tttatattat atctgccgtt tgatttcaga agaacgtcta
    10861 tatcgtttta ttgatcgaca cggtaagtgg attaaattga aaagtaagga tttaaagcga
    10921 gcaaatgatt ggtttaaaaa gtatggcgta tgggctgtat ttatctgtcg tttcatacct
    10981 gtattacgtg tattgattac cattccagct ggtgtaaatc gcatgaatgt tgtgacattt
    11041 accgttattt cattaatagg tactacaatc tggaatttcg gtttaatttt actaggacgc
    11101 actttgagcg atagctttgg tatgttgatg actggtcttc atacatattc acgtattatg
    11161 tatgttgtca tcattatcgc agtcatttac tttgctatac gttacattgg caaacgtaag
    11221 agagtaaaat aatattgttg ttagcataaa ggcatgaatt ttaaatttct ataaacaaaa
    11281 agcagtgaat cattccatct attgaaatga ttcactgctt ttttaagttt atttggcata
    11341 ctttagctga aagtcaacta tctttatata aagaaatatt actattaaca tgatagttat
    11401 agttgtgaag taaaaggcta ccaacggctg agaaatgata ccaattaata atgcaccgac
    11461 agtcattcca atatatctca caaaattaaa tacacctagc actttccctt ttatatgttc
    11521 aaacctttta gaaattatag tagacagcaa agctggcaca ataccaacac aagtacctaa
    11581 tataaatata attactgata atataattac atttgattcg tttaaccatc caaaca
//
LOCUS       DAXWFK010000078        11317 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.078, whole genome shotgun sequence.
ACCESSION   DAXWFK010000078 DAXWFK010000000
VERSION     DAXWFK010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11317
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.078"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            17..655
                     /locus_tag="ACGEXS_001580"
     CDS             17..655
                     /locus_tag="ACGEXS_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFF8012590.1"
                     /translation="MDDLNKKYLIDLHQHQNSSIEVLREFAEVNEVPIVDRLTLDLIK
                     QLIRMNNVKNILEIGTAIGYSSMQFASISDDIHVTTIERNETMIQYAKQNLATYHFEN
                     QVRIIEGNALEQFENVNDKVYDMIFIDAAKAQSKKFFEIYTPLLKHQGLVITDNVLYH
                     GFVSDIGIVRSRNVRQMVKKVQDYNEWLIKQPGYTTNFLNIDDGLAISIKGE"
     gene            658..1581
                     /locus_tag="ACGEXS_001581"
     CDS             658..1581
                     /locus_tag="ACGEXS_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFF8012591.1"
                     /translation="MTELLVTPKSLSHMETLIDLGADAFVIGEQKFGLRLPGEFNRQQ
                     MTEAVALAHKNDKKVYAAVNGLFHNYHLDAVEDYINFLHEIRVDRIIFGDPAVVMYVK
                     AQENPIPLHWNAETLVTNHFQCNYWGKRGASRAVLARELNLDEIINIKENSNVEIEVQ
                     VQGMTCMFQSKRMLLGNYYTFQDRQMKIERRNDEQSLLLYDEERQNNYPVYEDYNGTH
                     IMSPNDICLIEELAPFFEAGIDSFKIDGILQTEEYINVVTEQYRQAIDLYNEDPEIYE
                     DEKFMLMDPIEEIQPDHRPFDEGFLYKQTVY"
     gene            1593..2861
                     /locus_tag="ACGEXS_001582"
     CDS             1593..2861
                     /locus_tag="ACGEXS_001582"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFF8012592.1"
                     /translation="MKTIEEIKSTPKTVMKKPELLAPAGNLEKLKIAVHYGADAVFLG
                     GQEYGLRSNADNFTMEEIAEGVEFANRYGAKIYVTTNIIAHDENIEGLESYLRNLEKT
                     GATGIIVADPLIIETCKEVAPKLEIHLSTQQSLSNYKAVEYWKEEGLDRVVLARETGA
                     MEMREMKEKVDIEIEAFIHGAMCIAYSGRCTLSNHMTARDSNRGGCCQSCRWDYELLE
                     VDDNGELDVFYNQGEVTPFAMSPKDLKLIESIPQMMDIGVDSLKIEGRMKSIHYIATV
                     VSVYRKVIDAYAADPDNFKINPEWLIELDKCANRDTAPAFFEGTPGYEEQMFGQQQSK
                     KSPFDFCGLVLDYNEDTKIATIQQRNNFKPGQEIEFFGPEIETFTQVVEAIYDEEGNS
                     LDAARHPLQIVQIKVDRPIYPNNMMRKEIG"
     gene            2861..3484
                     /gene="udk"
                     /locus_tag="ACGEXS_001583"
     CDS             2861..3484
                     /gene="udk"
                     /locus_tag="ACGEXS_001583"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFF8012593.1"
                     /translation="MKATTIIGIAGGSGSGKTTVTNEIMKNLEGHSVALLAQDYYYKD
                     QKHLTFDERLETNYDHPFAFDNDLLIENLKDLKNGKAVEVPTYDYASHTRSDITIDFK
                     PKDVIIVEGIFALENKVLRDMMDVKIYVDTDADLRILRRLTRDTKERGRSMDSVINQY
                     LSVVRPMHDQFIEPTKKYADIIIPEGGSNKVAIDIMTTKIQSLVSKQ"
     gene            3512..3988
                     /gene="greA"
                     /locus_tag="ACGEXS_001584"
     CDS             3512..3988
                     /gene="greA"
                     /locus_tag="ACGEXS_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFF8012594.1"
                     /translation="MENQKQYPMTQEGFEKLERELEELKTVKRPEVVEKIKVARSFGD
                     LSENSEYDAAKDEQGFIEQDIQRIEHMLRNALIIEDTGDNNVVKIGKTVTFVELPGDE
                     EESYQIVGSAESDAFNGKISNESPMAKALIGKGLDDEVRVPLPNGGEMNVKIVNIQ"
     gene            4314..5048
                     /gene="pxpB"
                     /locus_tag="ACGEXS_001585"
     CDS             4314..5048
                     /gene="pxpB"
                     /locus_tag="ACGEXS_001585"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFF8012595.1"
                     /translation="MDVRFINEQTIMIYFENKISEETYRNVTAMVRWIREKEILEIQD
                     IVPSYRAVLIYFDEQAITSSKLIENLELNKFNEKNVHAVNQTNRIIKIPVQYGGTYGP
                     DIEEVAKHNRITVEQVIEKHTSKPYLIYMLGFMPGFPYLGGLDEQLHTPRRNQPRLKI
                     HAGSVGIANNQTGLYPSDSPGGWQIIGRTPLKVFSSEREPMSMYEAGEWIQFYAIDEQ
                     KFIQIERDISDGNFNVDDWVVIENVN"
     gene            5038..6048
                     /locus_tag="ACGEXS_001586"
     CDS             5038..6048
                     /locus_tag="ACGEXS_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HFF8012596.1"
                     /translation="MSIKILQPGLFSTVQDLGRIGYEHIGFSGAGAMDQFSFKVAQSL
                     INNDGPAIEYTLIGPTIQFNTQNTFVITGGSINASLNNKTISMNSVILAEKGDILKIG
                     AITKGARGYLTFGHSINVPSIAESYATHTRSSIGGFKGRKLLADDVITVKINNDFKEN
                     IGKTIHLQDDLLPENNIIHILQGPQFEAFSEEARAKIVNHPYLITEQSDRMGYRLEGD
                     SVAPINQADIISEPVALGSVQVPNDGQPIILLNDKQTIGGYTKIATVCKFDLPKLAQM
                     KPQDTIQFKWISFQEAVDKNREQMSLFNEILKSHQKTPIFDTSSLRHTSKKLATILKG
                     DL"
     gene            6050..6499
                     /locus_tag="ACGEXS_001587"
     CDS             6050..6499
                     /locus_tag="ACGEXS_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HFF8012597.1"
                     /translation="MNIEKIEQIIKLVKENDVKKFKYKNFEDEIEIDFTDSNHLAAHS
                     NQSNQSMNNNDLTASKANDNSDVSTNDYHDIKSPMIGTFFLQDSKELTEPIVNVGDKV
                     NKGDIIGYVEAMKVLNEVTTDVAGEITEIVADHGTNVEYDQVLVRIK"
     gene            6513..7874
                     /locus_tag="ACGEXS_001588"
     CDS             6513..7874
                     /locus_tag="ACGEXS_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HFF8012598.1"
                     /translation="MLRCLIANRGEIAVRIIRACREYGIETVAVYAKGDEQSLHVHLA
                     DQAICIGEANALDSYLNIDRIISAAQITGANAIHPGYGFLSESTKFAQTVEEQGIAFI
                     GPTKKTMEMMGDKITARQTVHHAGVPVIPGSNGAVNHVSEIENLAKDIGYPVVIKAAS
                     GGGGKGIRIVKKAEDLEKAFKEAKSEGKKYFDDDRVYVEAFIPVAKHVEVQVMGDGQD
                     NYVHLGERDCSVQRKNQKLIEESPCAALTEERRQQICNDAVKVARAANYRSAGTIEFL
                     VTDTAHYFIEMNARIQVEHTVTEMRAERDLVAAQLYLLEHNHLPFSQSDIQFNGHVIE
                     ARINAENPEKKFQPTPGKVTALHLPQGFNVRVDSLLYHGYQVSPYYDSLVAKVIVKSH
                     DRASAIDKLKVTLDEMVIDGFSTTADFLYAVLNYPLYRDGDAKDVDIKFLEKHQIVKG
                     VEL"
     gene            7874..8626
                     /gene="pxpA"
                     /locus_tag="ACGEXS_001589"
     CDS             7874..8626
                     /gene="pxpA"
                     /locus_tag="ACGEXS_001589"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFF8012599.1"
                     /translation="MRVDLNCDLGEAFGNYYFGGDHQIIPLITSANVACGFHAGDENV
                     MNETVKLAKAHNVAVGAHPGLPDLKGFGRRNIDISNDEIYNLMIYQLGALQGFCRIHQ
                     VKINHVKPHGALYQMGAKDREIANVIAQAVYDFDPSLVLVGLANSYLISEAKNVGLIT
                     ASEVFADRRYEDDGQLVSRKESDAVITDTDEALKQVLKMVKENKVISKNNKEVTLQAD
                     TICVHGDGEHALLFVSKIREILMKEGIDIQSL"
     gene            8639..9868
                     /locus_tag="ACGEXS_001590"
     CDS             8639..9868
                     /locus_tag="ACGEXS_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HFF8012600.1"
                     /translation="MGRNLKLKKESDFEFTKNHKRLLLGSVFLMATSAIGPAFLTQTA
                     VFTSQFFASFAFAILLSIIIDIGAQINIWRILVVTGLRGQEISNKVVPGLGTVISILI
                     AFGGLAFNIGNIAGAGLGLNAIFGLDVKWGAAITAIFAILIFVSKSGQKIMDVVSMIL
                     GIVMILVVAYVMFVSNPPYGDAFVHTFAPEHPMKLVLPIITLVGGTVGGYITFAGAHR
                     ILDSGIKGKQYLPFVNQSAIAGILTTGIMRTLLFLAVLGVVVTGVTLSSENPPASVFE
                     HAIGPIGKNIFGIVLFAAAMSSVIGSAYTSATFLKTLHKSLNERSNLIVIVFIVISTM
                     IFLFIGKPISLLIIAGAINGWILPITLGAILIASKKKSIVGDYKHPNWMFIFGIVAVL
                     VTILTGIFSFKEVLQLF"
     gene            10157..>11317
                     /locus_tag="ACGEXS_001591"
     CDS             10157..>11317
                     /locus_tag="ACGEXS_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFF8012601.1"
                     /translation="MGYRTLKSIFHEHNESKMKEEYIKRFNSLASFNTNINIIPMENG
                     KKVNDLEYPLFFMVTKNLSKKQELISINSRKIDRALNSLPYAAREQYFNDLLIDELQS
                     TNEIENVFSTKQEIAHALNNQASEFLKFRGLVDQYKEIELNKKIKVDNVRDIRAIYDK
                     LVSNEINEQDKLDGELFRKNFVGVHDGSTNKYIHVGLQPETKIVEFIGEMLTFLKYFD
                     APQPFKIMASHYLFEYIHPFYDGNGRVGRFIIAKLLSDYYDNYTALTFSYVINRNKSK
                     YYKAFMTASNHLNCGDLTEFIDTMLELLIAGQERILDELIPKMDATEKLTLYLTSHYK
                     QIDYEFLYLLSMDKLFGNKRNRLTLIDLENILGVGRVKINNTIKKYDNYLVKI"
BASE COUNT         4109 a         1516 c         2196 g         3496 t
ORIGIN      
        1 aaaggaagtt taaaacatgg atgacctaaa taaaaaatat ttaatagatt tacatcaaca
       61 tcaaaatagt tcaatcgaag ttttgcgtga atttgccgag gtaaatgaag tgccaattgt
      121 agatcgttta acattagatt taattaagca attaattcgt atgaataatg ttaaaaatat
      181 tttagaaatt ggtacagcaa tcggctatag ttctatgcaa ttcgcttcta tatctgatga
      241 cattcatgtc acaacgatag agcgtaatga aacgatgatt caatatgcta aacaaaattt
      301 agctacttat cattttgaaa accaggttcg aattattgaa ggtaatgctt tagagcaatt
      361 tgaaaatgta aatgacaaag tttatgatat gatatttatt gatgcagcaa aagcgcaatc
      421 aaagaaattt tttgaaatat atacaccact tttaaagcac caaggtctcg taattacaga
      481 taatgtttta tatcacggtt ttgtatcgga tattgggatt gttagatcga gaaatgtaag
      541 acaaatggtt aaaaaggttc aagattataa tgagtggtta ataaagcaac caggatatac
      601 aacgaatttt ttaaatatag acgatggatt agcgatttca attaaaggag aatgactatg
      661 acagaattac ttgtcacacc caagtcatta agccatatgg aaacacttat tgatttaggt
      721 gcagatgctt ttgtaattgg cgagcaaaag tttggtttaa gacttccagg ggaattcaat
      781 agacaacaaa tgacagaggc tgtcgcatta gctcacaaaa acgataaaaa agtatatgcg
      841 gcagtgaatg gattatttca taattatcat ttagatgctg tagaagatta tattaatttt
      901 ttacatgaaa tacgagttga cagaattatt ttcggggatc cagcagtagt aatgtatgtg
      961 aaggcgcaag aaaatccaat accacttcat tggaatgcag agacgttagt aactaatcat
     1021 tttcaatgta attactgggg taaaagaggc gcatcaagag ctgtcttagc acgtgagctg
     1081 aatttggatg aaattattaa tataaaagaa aattcaaatg tggaaattga ggtgcaagtt
     1141 caaggaatga cttgtatgtt tcaatcaaag cgtatgctac taggcaatta ttatacgttc
     1201 caagatcgcc aaatgaaaat tgaacgtcgc aatgatgaac aatctttatt actttatgat
     1261 gaagaaagac aaaataatta cccagtttac gaagattaca atggtacgca cattatgtca
     1321 ccaaacgaca tttgtttaat tgaagaatta gcaccatttt ttgaggcggg tatagattca
     1381 tttaaaatcg atggtattct acaaacggaa gaatatatta atgtggtaac ggaacagtat
     1441 cgtcaagcga tagatttgta caatgaagat cctgaaatct atgaagatga gaaatttatg
     1501 ttgatggatc caattgaaga aattcaacct gatcatcgac catttgacga aggtttctta
     1561 tataaacaaa cagtatatta aggaggttaa tcatgaagac aatagaagag attaaatcaa
     1621 ctcctaaaac agttatgaag aaaccagaat tattagcacc tgctggaaac ttagaaaagt
     1681 taaaaatagc agtacattat ggcgctgatg ccgtattttt aggtggtcaa gaatatggat
     1741 tacgttcaaa tgctgataat ttcacgatgg aagaaatagc tgaaggtgtt gaatttgcga
     1801 accgttacgg tgccaaaatt tatgttacga caaatattat tgcacatgat gagaatattg
     1861 aaggtctaga atcatatttg cgtaatttgg aaaagactgg tgcgacaggt atcattgttg
     1921 cagatccttt aattatcgaa acatgtaaag aagttgcgcc aaaacttgaa attcatttat
     1981 ctactcaaca atcactttct aattacaaag ctgtagaata ttggaaagaa gaaggattgg
     2041 atcgtgttgt attagcacgt gagaccggcg cgatggaaat gcgtgaaatg aaggaaaaag
     2101 tagatattga aatcgaagca tttattcatg gtgctatgtg tatcgcctat tcaggtagat
     2161 gtacattaag taatcatatg actgcaaggg attccaacag aggcggttgc tgtcaaagtt
     2221 gccgttggga ttatgaatta ttagaagttg atgataatgg tgaacttgat gttttttata
     2281 atcaaggtga agttacaccg tttgcgatga gtcctaaaga tttaaaatta atcgaatcaa
     2341 ttcctcaaat gatggatatt ggtgtggact cattaaaaat tgaaggacgt atgaagtcaa
     2401 ttcattatat tgcaacagtt gtctcagtat atcgtaaagt cattgatgcg tatgcggcag
     2461 atcctgacaa ctttaagatt aatccggaat ggttaataga gttagataaa tgtgcaaata
     2521 gagacactgc accagcattc tttgagggaa cacctggtta tgaagaacag atgtttggtc
     2581 aacaacaatc taaaaaatca ccatttgatt tttgtggttt ggtattagac tataatgaag
     2641 atacaaaaat tgcgactatt caacaacgaa ataactttaa accaggtcaa gaaatagaat
     2701 tctttggtcc agaaattgaa acatttacac aagtagtaga agcaatttat gatgaagaag
     2761 gtaacagctt agacgcggcg cgccatccat tacaaatcgt tcaaattaaa gtggatcgcc
     2821 cgatatatcc aaacaacatg atgagaaagg aaattggcta atgaaagcta ctacaatcat
     2881 tggcatagct ggtggatctg gctcaggaaa aacaactgta actaacgaaa ttatgaaaaa
     2941 cttagaaggt catagtgtcg ctttacttgc tcaagattac tattataaag atcaaaagca
     3001 cttgactttc gacgagcgcc tagaaaccaa ttatgaccat ccatttgcat tcgataatga
     3061 tttattaatt gaaaatctta aagacttgaa aaatggtaaa gcagtagaag taccgacata
     3121 tgattatgct agtcatacaa gaagtgacat taccattgat tttaaaccta aagatgttat
     3181 tatcgtagaa ggcattttcg ctttagaaaa taaggtatta cgtgatatga tggatgttaa
     3241 aatatatgtt gatacagatg cagacttgag aatattacgc cgtttaacac gagatactaa
     3301 agagcgtggg cgttcaatgg actctgttat caatcaatat ttaagtgttg ttagacctat
     3361 gcatgaccaa tttattgaac cgactaagaa atatgctgat ataattattc ctgaaggtgg
     3421 gagcaataaa gttgcaatag atattatgac aacaaaaatt cagtctttag ttagcaagca
     3481 atagtataat tcagtaaagg aagatgacaa tatggaaaat caaaagcaat atccaatgac
     3541 tcaagaaggt tttgaaaaat tagagcgtga acttgaagaa ttaaaaacag ttaagcgtcc
     3601 tgaagttgta gagaaaatta aagttgcacg ttcatttggt gacttatcag agaactctga
     3661 gtatgatgca gcaaaagatg aacaaggatt catcgaacaa gatattcaaa gaattgagca
     3721 tatgttaaga aatgcattaa tcattgaaga tactggagat aacaacgttg ttaaaattgg
     3781 taaaacagta acgtttgtag aattaccagg tgatgaagag gaaagttatc aaatcgttgg
     3841 ttcagctgaa tcagatgcat ttaatggtaa gatttcaaat gaatcaccaa tggctaaagc
     3901 gttaattggt aaaggtttag atgatgaagt tcgtgttcca ctacctaatg gtggcgaaat
     3961 gaacgtaaaa attgttaata tccaataaat gatcaaatag attatttaat tgcgcgattg
     4021 aagtgtgtta atgatacttc ggtcgtgctt ttttatattc aaaatcaaaa aatcggacag
     4081 atgaaaagta aaaattttcc atcagaccga atgatagcag aacctaaatt agtgtaagag
     4141 ttacaatatt aactactcca ttttaaaaaa gcaaaatatt tccgagaaaa caaattgatt
     4201 atcatttgtt attgtatgcg atttgaatta cttattaaac aacttgtgct tctcaatgat
     4261 acaatgaaaa ggtattcaat actggtttat tgcaatacaa agggggattt aaagtggatg
     4321 taagatttat taacgagcag acaattatga tttattttga aaataaaata tccgaagaga
     4381 catatcgtaa tgtcacagca atggtacggt ggatacgaga aaaagaaata ttagaaattc
     4441 aagatattgt cccttcgtat cgagcagttc taatatattt tgatgaacaa gctataacat
     4501 cttctaaatt aattgaaaat ttagaactta acaaatttaa tgaaaaaaat gttcatgctg
     4561 tgaatcaaac caatcgtatt attaaaatac ctgttcaata tggtggtaca tatggaccag
     4621 atattgaaga agtagcaaag cataatcgaa taactgttga gcaagttatt gaaaaacata
     4681 caagtaaacc ttatttaata tatatgctag gatttatgcc aggatttcca tacttaggcg
     4741 gacttgatga acaactacat acacctagac gtaatcagcc aaggttaaaa attcatgcag
     4801 gttctgtagg tattgcgaat aatcaaacag gactatatcc atcagattct cctggcggat
     4861 ggcaaattat tggacgcaca ccgttaaaag tatttagcag cgagcgtgaa ccaatgtcaa
     4921 tgtacgaagc aggagaatgg atacaatttt atgcaataga tgaacagaaa tttatacaaa
     4981 tcgaaagaga tatatcagat ggcaacttta atgttgatga ctgggtggtg attgaaaatg
     5041 tcaattaaga ttttacaacc aggtctcttt tcaacggtac aagatctagg aagaataggt
     5101 tatgaacata ttggattttc aggcgcaggt gccatggatc aatttagttt taaggttgcg
     5161 cagtcattaa ttaacaatga tggtccagcg attgaatata ctttgattgg tcctaccatt
     5221 caatttaata cgcaaaatac atttgttata accggtggta gtattaatgc ttcgctaaat
     5281 aataaaacta tatcaatgaa ttctgtcata ttagctgaga aaggtgacat tttaaaaata
     5341 ggtgctataa ccaaaggtgc acgcggttat cttacttttg gtcattctat caacgtacct
     5401 tctattgctg aaagttatgc aacacatacc cgaagcagta taggaggatt caaaggtaga
     5461 aagttattag ctgatgatgt aataacagtg aaaataaata atgactttaa agaaaacatt
     5521 ggaaagacga ttcatttaca ggacgattta ttgccagaaa ataatattat acacattctt
     5581 caaggacctc aattcgaggc attttctgaa gaggctagag cgaaaattgt aaatcatcca
     5641 tatttaatta ctgaacaatc agaccgtatg ggctatcgtt tggaaggtga cagcgttgca
     5701 ccaattaatc aagcagatat catttctgaa ccggttgctc ttggtagtgt tcaggtacca
     5761 aatgatggtc aacctattat tctgcttaat gataaacaaa cgataggtgg ttataccaaa
     5821 attgcaactg tatgcaaatt tgatctgcca aagttagcac agatgaaacc acaagataca
     5881 attcaattta aatggatatc attccaagaa gctgtagata agaatcggga acaaatgtct
     5941 ctgtttaatg aaattcttaa gagtcatcaa aagactccaa tttttgatac atctagcttg
     6001 cgacatactt cgaaaaaatt agcaacaatt ttaaaagggg atttgtaaaa tgaatattga
     6061 aaaaatcgaa caaataatca aattagtgaa ggaaaatgat gttaagaaat ttaaatataa
     6121 aaattttgaa gatgaaatag aaattgactt cactgactcg aatcatttgg ctgcacacag
     6181 taatcaatca aatcaaagta tgaacaataa tgatttgaca gcttcaaaag cgaatgataa
     6241 ctccgatgtt tcgacaaatg attatcatga cattaaatca ccaatgatag gtacattctt
     6301 tttacaagat agtaaagaat taactgaacc aattgtgaat gtcggtgaca aagttaacaa
     6361 gggagatatt ataggatatg ttgaagcgat gaaagtatta aacgaggtaa caacagatgt
     6421 tgctggagaa attactgaaa tagtagctga tcatggcaca aatgttgaat acgaccaagt
     6481 tttggtacgt attaagtaaa gggggtaagt aaatgcttcg ttgtttaatt gcgaacagag
     6541 gtgaaattgc tgtaaggatt ataagagctt gcagagaata tgggattgaa actgtagcag
     6601 tttatgcaaa aggggacgag caaagcttgc atgtacattt agcagatcaa gctatatgta
     6661 ttggggaagc taatgcttta gatagttatt taaatattga ccgcatcata tctgctgcac
     6721 aaatcactgg tgctaatgca attcacccag gatatggctt tttatcagaa tcaacaaaat
     6781 ttgctcaaac cgttgaggaa caaggcatag cgtttatagg gcctactaaa aaaacgatgg
     6841 aaatgatggg ggataaaata acagcaagac aaacagttca tcatgcaggt gttcctgtga
     6901 ttccaggatc aaatggtgct gtgaatcatg tgtctgaaat tgagaatctt gccaaagaca
     6961 tcggttatcc agttgttatc aaagctgcca gtggcggtgg tggtaaaggt atacgtattg
     7021 taaagaaagc tgaagattta gaaaaggcat ttaaagaagc taaaagtgaa ggaaaaaaat
     7081 actttgatga tgatcgtgtt tatgttgagg cttttatacc tgttgcaaaa catgttgaag
     7141 ttcaagttat gggagatggg caggataatt atgtacattt aggggaacgt gattgttctg
     7201 tacaacgtaa aaatcaaaaa ttaatcgaag aatctccttg tgcagcatta actgaagaaa
     7261 gacgacaaca aatatgtaac gatgctgtta aagttgcacg tgcagcaaat tatagaagtg
     7321 caggtacgat tgaattttta gtaactgata ccgcacatta ctttattgaa atgaatgctc
     7381 gaatacaagt tgaacatact gttacagaga tgcgagcaga acgtgatttg gttgcagcac
     7441 aactgtattt attggaacat aatcatttac ctttttcaca aagcgatatt caatttaatg
     7501 gtcatgtgat agaagcgaga ataaatgccg aaaatcctga aaagaaattt caaccaacac
     7561 caggtaaagt gacagcgtta catttacctc aaggctttaa tgtgcgagtc gattctttac
     7621 tatatcacgg ttatcaagtt tcaccatatt atgattcttt agtagctaaa gtaattgtaa
     7681 aatctcatga tagagcttca gcaattgata agttgaaagt caccttagat gaaatggtga
     7741 tagatggatt ttctacaact gccgattttt tatatgcagt tttaaattat ccactatatc
     7801 gtgatggaga tgcaaaagat gttgatatta aatttttaga aaaacatcaa attgttaaag
     7861 gggtggaatt ataatgcgag ttgatttgaa ttgtgattta ggcgaagcat ttggaaatta
     7921 ttactttggt ggtgatcatc aaattattcc gttaattaca agtgcgaatg ttgcttgtgg
     7981 ttttcacgct ggtgatgaaa atgtaatgaa tgaaacggta aaacttgcca aagcacataa
     8041 tgttgcagta ggtgcacatc ctggtttacc tgatttgaaa ggctttggca gacgaaatat
     8101 agatatctct aacgacgaga tttataattt gatgatttat caattaggtg cattacaagg
     8161 gttttgtcgc attcatcaag ttaaaattaa tcatgttaaa ccgcatggtg cattgtatca
     8221 gatgggtgca aaagacagag aaatagcaaa cgttatagca caagctgttt atgactttga
     8281 tccatcacta gtgttagtag gattagcaaa ttcatatcta atttcagaag caaagaatgt
     8341 cggattaatt acagcttctg aagtgtttgc tgatagacga tacgaagatg atgggcagct
     8401 cgttagtaga aaagaaagtg atgctgtgat tactgatact gacgaagcac ttaagcaggt
     8461 tttaaagatg gtgaaggaaa ataaagttat ttcaaaaaac aataaggaag taacgttaca
     8521 agcagataca atttgtgtgc atggtgatgg agaacatgca ttattatttg tttcgaaaat
     8581 tagagaaatt ttaatgaaag aaggcattga tattcaatcc ttataggggg ctaaaaaaat
     8641 ggggagaaat ttaaagctta aaaaagaaag tgactttgaa ttcacaaaaa atcataaaag
     8701 gttattatta ggttctgtat ttttgatggc aacttctgca attgggccag catttttaac
     8761 gcaaacagca gtatttacat cacaattttt tgcaagtttc gcatttgcca tattactgtc
     8821 tatcatcatt gacattggtg cacaaattaa tatatggcgc atattagttg taactggttt
     8881 aagaggtcaa gaaatatcaa ataaagttgt tcctgggctt ggtactgtta tctcaatatt
     8941 gattgcattt ggtggtctag cttttaatat tggtaatatt gctggtgccg gtttaggttt
     9001 aaatgcaatt tttggattag atgtaaaatg gggcgcagct attactgcaa tctttgcaat
     9061 attaatcttt gtaagtaaaa gtggccaaaa aattatggac gttgtttcaa tgattcttgg
     9121 tattgtgatg attttagttg tggcatatgt gatgtttgtt tctaatccac cttatggtga
     9181 tgcttttgtg catacatttg cgccagaaca tccaatgaaa ttagtcttgc ccatcattac
     9241 gttagttggt ggaactgtag gtggttatat tacctttgca ggtgcacatc gtatattaga
     9301 ctctggcatt aaaggtaagc aatatttacc atttgtaaat caatcagcaa ttgctggtat
     9361 tttaactaca ggtattatga gaacgttact attcctagcg gtattaggag ttgttgtaac
     9421 aggtgtgaca ctaagttctg aaaatccacc agcgtcagtt tttgaacacg caattggacc
     9481 aattggaaag aatatttttg gtattgtgtt atttgctgca gctatgtcat cagtaattgg
     9541 ctcagcatac acaagcgcaa catttttaaa aacacttcat aaatcactta acgaaagaag
     9601 taatttaatt gtgattgtgt ttatcgttat ttcaacaatg attttcttat ttattggaaa
     9661 accaatcagc cttttaatta tagcaggcgc gataaatgga tggattttac ctattacgtt
     9721 aggtgcaatt ttgattgcaa gtaaaaagaa atcaattgtt ggtgattata aacacccaaa
     9781 ttggatgttc atctttggta tcgttgcagt acttgttaca atattgacag gtatattctc
     9841 attcaaagaa gtacttcagt tattttaaaa gacatttcga ctaatagcat cactgtaaat
     9901 gttgttagta ttaggcaaga tatattttga tttccggtga ttcatttata aagcataatt
     9961 agatatattt taagggagag tggtgcatga caaatgtact gctctttttg ttgtgatgaa
    10021 aaatcttttt ctattgattg agaaaatagt tatttatata gtttttaaag aaaaaaagtg
    10081 ataatattta gctaatctaa tgaaaattga tttattggac aaaaaataca ttttaaatga
    10141 gtagaggagg ctgcaaatgg gttacagaac tttaaaaagt atttttcatg aacacaatga
    10201 aagtaaaatg aaggaagagt atattaaaag atttaattct ttagcttctt tcaatactaa
    10261 tattaatatc atacctatgg aaaatggaaa aaaagttaat gatttggaat atcctctatt
    10321 ctttatggtg actaaaaatc tatcaaaaaa acaagaatta atatcaatta atagtagaaa
    10381 aattgataga gcacttaatt ctttaccata tgcagctaga gaacaatatt ttaatgattt
    10441 attaatcgat gaattacaaa gtactaatga aattgaaaat gtatttagta ctaaacaaga
    10501 gattgcacat gcgttaaata accaagcatc agaatttctt aagttcagag gcctcgtgga
    10561 tcaatataaa gagatagaac ttaataaaaa aattaaagtt gataatgtaa gagacattag
    10621 agcgatttat gataaattag tttcaaatga aattaacgaa caagataagt tagatggaga
    10681 gctatttcgt aaaaattttg tcggtgtgca tgatgggtca acgaataaat atatacatgt
    10741 tgggttacaa cctgaaacca aaattgttga atttataggt gaaatgctaa catttttaaa
    10801 atattttgat gcgcctcagc cgttcaaaat catggctagt cattatctgt ttgaatatat
    10861 acatcctttt tatgatggca atggtagagt tggaagattt ataattgcca agctcttgag
    10921 tgattattat gataattata ctgctttgac attttcatat gttataaata gaaataaatc
    10981 gaaatattac aaggcgttta tgactgcttc aaatcatttg aattgtggtg atttgacgga
    11041 atttattgac acaatgcttg agctattgat agcaggacaa gaaagaatac ttgatgagtt
    11101 aataccaaaa atggatgcga ctgaaaaatt aacgctttat ttaactagtc attataaaca
    11161 gatagattat gaatttttgt atctcctttc aatggataaa ttgtttggaa ataaaagaaa
    11221 tagattaaca ttaattgatt tagaaaacat attgggagta ggaagagtaa aaattaataa
    11281 tacaattaaa aaatatgata actacttagt taaaatt
//
LOCUS       DAXWFK010000079        11316 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.079, whole genome shotgun sequence.
ACCESSION   DAXWFK010000079 DAXWFK010000000
VERSION     DAXWFK010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11316
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.079"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            2..934
                     /gene="hlgC"
                     /locus_tag="ACGEXS_001592"
     CDS             2..934
                     /gene="hlgC"
                     /locus_tag="ACGEXS_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgCB subunit C"
                     /protein_id="HFF8012602.1"
                     /translation="MLTTTLSVSLLAPLANPLLENAKAANDTEDIGKGSDIEIIKRTE
                     DKTSNKWGVTQNIQFDFVKDKKYNKDALILKMQGFISSRTTYYNYKKTNHVKAMRWPF
                     QYNIGLKTNDKYVSLINYLPKNKIESTNVSQTLGYNIGGNFQSAPSLGGNGSFNYSKS
                     ISYTQQNYVSEVEQQNSKSVLWGVKANSFATESGQKSAFDSDLFVGYKPHSKDPRDYF
                     VPDSELPPLVQSGFNPSFIATVSHEKGSSDTSEFEITYGRNMDVTHAIKRSTHYGNSY
                     LDGHRVHNAFVNRNYTVKYEVNWKTHEIKVKGQN"
     gene            936..1913
                     /gene="hlgB"
                     /locus_tag="ACGEXS_001593"
     CDS             936..1913
                     /gene="hlgB"
                     /locus_tag="ACGEXS_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgAB/HlgCB subunit
                     B"
                     /protein_id="HFF8012603.1"
                     /translation="MKMNKLVKSSVATSMALLLLSGTANAEGKITPVSVKKVDDKVTL
                     YKTTATADSDKFKISQILTFNFIKDKSYDKDTLVLKATGNINSGFVKPNPNDYDFSKL
                     YWGAKYNVSISSQSNDSVNVVDYAPKNQNEEFQVQNTLGYTFGGDISISNGLSGGLNG
                     NTAFSETINYKQESYRTTLSRNTNYKNVGWGVEAHKIMNNGWGPYGRDSFHPTYGNEL
                     FLAGRQSSAYAGQNFIAQHQMPLLSRSNFNPEFLSVLSHRQDGAKKSKITVTYQREMD
                     LYQIRWNGFYWAGANYKNFKTRTFKSTYEIDWENHKVKLLDTKETENNK"
     gene            complement(1965..2432)
                     /locus_tag="ACGEXS_001594"
     CDS             complement(1965..2432)
                     /locus_tag="ACGEXS_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFF8012604.1"
                     /translation="MTSKDITQISVIAAILTILAVLKIPSIIPGLDFQLSAPAALLIL
                     AFFGIKKYFLGGLLSSLLLLVFGVFNPINVIISIIFRVIAIAVVYLLKINVLSLVLAS
                     VLGSLVSRLLLSIILNLPVWVVLLNAIPGVIFTLIVAIPLYLTLRKRMAVLLR"
     gene            complement(2443..3135)
                     /locus_tag="ACGEXS_001595"
     CDS             complement(2443..3135)
                     /locus_tag="ACGEXS_001595"
                     /EC_number="6.2.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxyhexanoate--CoA ligase"
                     /protein_id="HFF8012605.1"
                     /translation="MYSIKMRSSNQDVHISGAETICEFDKIEQTVQRFYNKGFFHENG
                     QPDFLNIKIQKIMEPIQQIKALQIIEDDKANLQHLTQECGVTEQALNQGMTYIKNETV
                     YTGAIILSAISGKRLDSFGQRGIRATHFSFEDINNKGDLNERVTDALAIASCINAHPY
                     VKGELCVSDDLTYTTGYFAAAKIGYHRLFDIKPVNTRYGGRIIFVDDCIDLNHYISFL
                     ESTPKQVVYETV"
     gene            complement(3146..4261)
                     /locus_tag="ACGEXS_001596"
     CDS             complement(3146..4261)
                     /locus_tag="ACGEXS_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFF8012606.1"
                     /translation="MKFQEQIDLLQQKGLYRSLKSVGYIDRQYIEVENKRCTNYTSND
                     YLGLGQIAFDKDDFERFMRKYSYHLSSSRLISGSSTAYEEIETMLAGWLGYSACTILN
                     SGYDANLALFNIFKNTNCVVFSDQENHASIIDGIKLSGLEKVIYKHLDIADLEKRLEK
                     YPNQNIPKIIISDSVFSTNGDVVDIGQLVSLKHKYNATLILDVSHSFGIENYSNYQGV
                     DILTSSLSKACGAYGGVILSSNDVKDMLINHGRPLIYSSSLPIYNLYFIKRNIEKLIN
                     ADDRRTKLNSLSKYFNQKLKALNVNYNSSNSPIKFIEFDDIEAAENIHQTLLKHHVFT
                     SYLRYPTVTKPMLRISLSYFHTEQDIDRLFEILHQED"
     gene            complement(4239..5246)
                     /gene="bioB"
                     /locus_tag="ACGEXS_001597"
     CDS             complement(4239..5246)
                     /gene="bioB"
                     /locus_tag="ACGEXS_001597"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFF8012607.1"
                     /translation="MNLAKRILQGEQLTKETVLKIYEDTNIDTLDLLNEAYILRKHYF
                     GKKVKLNMILNAKSGICPENCGYCGQSRDIKQKQRYALIPEEQIIDGAKVAHDNHIGT
                     YCIVMSGRGPSDKEVDHISNTVRTIKSQHPQLKICACLGLTNDEQAKKLKSAGVDRYN
                     HNINTSENYHDNVVTTHSYKDRTDTIELMKANNISPCSGVICGMGESNQDIVDMAFAL
                     KEMDADSIPINFLHPIKGTKFGSMDDLTPMKCLRIVALFRLINPTKEIRIAGGREVNL
                     RSLQPLALKAANSIFVGDYLITGGQPNQLDYDMINDLGFEIDYDTCENKENKNEVSRA
                     N"
     gene            complement(5248..6606)
                     /gene="bioA"
                     /locus_tag="ACGEXS_001598"
     CDS             complement(5248..6606)
                     /gene="bioA"
                     /locus_tag="ACGEXS_001598"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFF8012608.1"
                     /translation="MNYTQQLKQKDSEYVWHPFTQMGVYSKEEAIIIEKGKGSYLYDT
                     NGNKYLDGYASLWVNVHGHNNKYLNKVIKKQLNKIAHSTLLGSSNIPSIELAEKLIEI
                     TPSNLRKVFYSDTGSASVEIAIKMAYQYWKNIDREKYAKKNKFITLNHGYHGDTIGAV
                     SVGGIKTFHKIFKDLIFENIQVESPSFYRSNYDTENEMMTAILTNIEQILIERNDEIA
                     GFILEPLIQGATGLFVHPKGFLKEVEKLCKKYDVLLICDEVAVGFGRTGKMFACNHED
                     VQPDIMCLGKAITGGYLPLAATLTSKKIYNAFLSDSHGVNTFFHGHTYTGNQIVCTVA
                     LENIRLYEKRKLLSHIETTSSTLEKQLHALKRHRNVGDVRGRGLMFGVELVTDKDSKT
                     PLEIEKVERIVRNCKENGLMIRNLENVITFVPVLSMSNKEVKTMVRIFKKAVHNILDR
                     KC"
     gene            complement(6584..7270)
                     /gene="bioD"
                     /locus_tag="ACGEXS_001599"
     CDS             complement(6584..7270)
                     /gene="bioD"
                     /locus_tag="ACGEXS_001599"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFF8012609.1"
                     /translation="MRIFITSTNTDVGKTYVTKHLYHALKTRGHRVCIFKPFQTEERQ
                     DGTFPDLEVFKNECDLSYDITSLYTFKQPVSPHLAFKMTDQIFLNKQRVLDKVKVLDK
                     EFDFILIEGAGGIAVPIYEGTDDFYMTKDLINDCADCVISVLPSKLGAISDAIVHQDY
                     VNQNVSASNFLIMNRYTDSYIEKDNQMTIGKLTNKTVYTFEEHATYENFSEAFLKQLI
                     GVKNELHTTT"
     gene            complement(7324..7491)
                     /locus_tag="ACGEXS_001600"
     CDS             complement(7324..7491)
                     /locus_tag="ACGEXS_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012610.1"
                     /translation="MQLLILTDIFYRIAVYICPNSLRSLSHFNSKVKILPKFIVILRS
                     LNSCFLEKHAP"
     gene            complement(7722..9455)
                     /locus_tag="ACGEXS_001601"
     CDS             complement(7722..9455)
                     /locus_tag="ACGEXS_001601"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8012611.1"
                     /translation="MFQITFKILNWIRPYKARMILGFSMSFLNAIFIALPIFLAAKIF
                     NNVLSHKPIYMKDILNVVIIMVLLVIGRFITAYFKSKSHESIAYEMSAKERLDIGDKL
                     KNVRLGYFNSHHSNELTTIVTTDLTFLENFAMKMVDVVVNGYILITVLILSLLVVSWQ
                     VSLLACIGVLLSFFAIQLLERKSRQNAPAYHNVQNQLVEKVLEVIRGIQVIKSFAKEN
                     TSLKSFNQAVNESKRINTKIEMQYIPFNLLHLLSLKVVSIMIVLVACLLYMNHSIDLP
                     TLIMISIFSFVIFDSVENINSAAHVLEMIDMTIDDIEKIKNAPELDENGKNLTIKNEN
                     IAFQNVNFSYDDKQVIKNVNFEIPTQTSTAIIGPSGSGKSTLCHLLLRFYDIDDGNIR
                     IDGVDIKDMTLSTLMSKISAVFQKVYLFNDTIENNILFGNPGATKEEIIRAAKQACCH
                     DFIMSLPEGYQTMLNEKGSNLSGGEKQRISIARAILKDAPIIILDEATASIDPENEQL
                     IQTAINELSKGKTVITIAHKLETIKNADQIIVLNEGEIIQKGSHDELIRKPGMYQDFI
                     RIKSKSAGWKL"
     gene            complement(9480..11243)
                     /locus_tag="ACGEXS_001602"
     CDS             complement(9480..11243)
                     /locus_tag="ACGEXS_001602"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8012612.1"
                     /translation="MNNQTNWIKILSGFASDSKWKIMLSILLSIISVFSGLVPYWAVF
                     KIILMMINNTYTINSIMVYIFIALIAYISQVCCFGASTMLSHITAYEILSEIRKKLAQ
                     KLMRLPLGVVESKKIGELKNIFVDKVETIELPLAHMIPEVIGNLLLSAAIFLYIMLID
                     WRMASALLVTIPISIFAFKKVMSGFNETYAEQMKSNNYMNSAIVEFIEGIEVIKTFNQ
                     SQSSYKKYKDAVDNYKIHTLNWFKNTWGYMNLGASVLPSTFLGILPVGMYLISINQLN
                     YAEFFLCIVLSLGVVAPIKNFTNYVNHLKSIQYALTEVNQILSLEELVLSTKFKKPQH
                     YEIAFNNVGFSYNKDKDDLVFKHLSFTVPENNFTAIVGASGSGKSTIAKLISRYWDVT
                     SGEITIGGINIKDIESKQLNDLVGFVGQDNFLLNLTFKENIKLGNPEATDEAVEKAAK
                     LAQCHEFIEKLPDGYDTNVGTVGDKLSGGEKQRVTIARMILKDAPIIVLDEATVYVDP
                     DNEQKIQEALNVLTQDKTLIVIAHRLSTIQHADQIIVLGKQQILEKGSHHLLLKLNGN
                     YKKMWDTHMHTKDWGINTGHN"
BASE COUNT         3756 a         2053 c         1532 g         3975 t
ORIGIN      
        1 aatattaact acaactttat ctgtgagctt acttgcccct cttgccaatc cgttattaga
       61 aaatgctaaa gctgctaacg atactgaaga catcggtaaa ggaagcgata tagaaattat
      121 caaaaggaca gaagataaaa caagtaataa atggggcgtg actcaaaata ttcaatttga
      181 ttttgtaaag gataaaaaat ataacaaaga tgctttgata ttaaagatgc aaggattcat
      241 tagctctaga acaacatatt acaactataa aaaaactaat catgttaaag ctatgcgatg
      301 gccattccaa tataatattg gtttaaaaac aaatgataaa tatgtttctt taattaatta
      361 tttacctaaa aataaaattg aatctacaaa cgtgagtcag acattaggat acaatatcgg
      421 tggtaatttc caatcagccc catcactcgg tggtaatgga tcatttaact attctaaatc
      481 gattagctat acacaacaaa attatgtaag tgaagtagaa caacaaaact caaaaagtgt
      541 tttatggggc gtcaaagcga attcattcgc cactgaatca ggtcaaaaat cagcctttga
      601 tagcgattta tttgtaggct acaaacctca tagtaaagat cctagagatt atttcgttcc
      661 agacagtgag ttaccacctc ttgtacaaag tggatttaac ccttcattta tcgccacagt
      721 atctcatgaa aaaggttcaa gcgatacaag cgaatttgaa attacttacg gaagaaacat
      781 ggatgtcact catgccatta aaagatcaac gcattatggc aacagttatt tagacggaca
      841 tagagtccat aatgcatttg taaatagaaa ctatactgtg aaatacgagg tcaattggaa
      901 gactcatgaa atcaaggtga aaggacagaa ttgatatgaa aatgaataaa ttagtcaaat
      961 catccgttgc tacatctatg gcattattat tactttctgg tactgctaat gctgaaggta
     1021 aaataacacc agtcagcgta aaaaaagtcg atgacaaagt tactttatac aaaacaacag
     1081 ccacagcaga ttctgataaa tttaaaattt cacagatttt aacatttaat ttcatcaaag
     1141 ataaaagtta tgataaagat actttagtac ttaaagctac tgggaatatt aactcaggct
     1201 ttgtgaaacc taatcctaat gactatgact tttcaaaatt atattgggga gctaaataca
     1261 atgtatctat aagctcacaa tctaatgatt cagtaaacgt cgttgattat gcaccaaaaa
     1321 atcaaaatga agagtttcaa gttcaaaata ctttaggcta tacatttggt ggtgacatta
     1381 gtatctctaa tggtttatct ggtggactta atggaaatac agctttttct gaaacaatta
     1441 attataaaca agaaagttac agaacaacat taagtcgcaa cacaaattat aaaaatgttg
     1501 gctggggagt tgaagcacat aaaattatga ataatggttg gggaccttat ggaagagata
     1561 gcttccaccc aacatatggt aatgaactct tcttagctgg cagacaaagc agtgcatacg
     1621 ctggccaaaa cttcatagcg caacaccaaa tgccattatt atctagaagt aacttcaatc
     1681 cagaattttt aagcgtacta tcacacagac aagatggcgc taaaaaatct aaaattacag
     1741 taacttatca acgtgaaatg gatttatacc aaattcgttg gaatggcttc tactgggcag
     1801 gcgcaaatta taaaaacttt aaaactagaa catttaaatc aacatatgaa attgattggg
     1861 aaaatcacaa agtgaaattg ttagatacaa aagaaactga aaacaataaa tagctagtaa
     1921 aacacggtcg ccaacagtaa ttgtgacgac cgtgttttga tttattatct tagtaatact
     1981 gccattcttt ttctcaatgt gagatataaa ggaatagcta caattaaagt gaatattacg
     2041 cctggaatcg cgtttaacaa cactacccac acaggtaaat ttaaaataat agataatagt
     2101 agcctagata ccaaactgcc taatacactt gctaaaacta atgatagtac atttattttc
     2161 aataaataaa caactgcaat agctataact ctaaatataa tagagataat cacattaatt
     2221 ggattaaata cgccaaatac tagtaataat aggctagata ataatccacc taaaaagtac
     2281 tttttaattc caaagaaagc taatatcaat aatgctgccg gtgcagataa ttgaaaatct
     2341 aatcctggta taatggacgg tattttcaaa actgccaaaa tggttaaaat cgcagcaatg
     2401 acactaattt gagtaatatc ttttgatgtc atactaaaac ccctataccg tttcataaac
     2461 aacttgcttc ggtgtgcttt ctaaaaatga tatgtaatga tttaaatcaa tacaatcgtc
     2521 cacaaatatt attctgcctc catatctcgt attaactggt ttaatatcaa ataatcgatg
     2581 gtaaccaatt ttagcagcgg caaaataacc tgtcgtatac gttaagtcat cggacacgca
     2641 aagttctcct ttgacatacg gatgcgcatt gatacaacta gcaattgcta aggcatcagt
     2701 cactctttca ttaagatcac ctttattatt tatatcttca aacgaaaaat gtgttgccct
     2761 aatccccctt tgtccaaatg aatctaaacg tttaccagat atagcagata gaataatagc
     2821 tcctgtataa accgtttcat ttttaatata tgtcatccct tgatttagcg cttgttcagt
     2881 gacaccacat tcttgtgtta aatgttggag atttgcttta tcatcctcaa taatttgtaa
     2941 tgcttttatt tgttgaatcg gttccatgat tttttgtatt tttatattga gaaaatccgg
     3001 ttgcccattt tcatgaaaaa aacctttatt ataaaacctt tgtaccgtct gttctatttt
     3061 gtcaaactca caaatcgttt cagcaccact aatatgaaca tcttgattgc tagaacgcat
     3121 ttttatacta tacatgacga tcacctcaat cttcttgatg caaaatttca aacaacctat
     3181 ctatatcttg ttcagtatga aaatacgaca atgatattct taacattggc ttagtcacag
     3241 ttggatacct taaataactt gtaaacacat gatgctttaa taatgtttga tgaatgttct
     3301 cagccgcttc tatgtcatca aactcaataa acttaatcgg cgagtttgaa ctattataat
     3361 taacattgag tgcttttaac ttttggttaa aatatttact caaactattt aatttagtgc
     3421 gtctatcatc agcatttatt aacttttcaa tgtttctttt tataaaatac aaattataaa
     3481 ttggcaaact acttgagtag atgagtggtc taccgtgatt aattaacata tccttcacat
     3541 catttgaact taaaatcaca cccccgtatg caccacatgc tttagataaa ctagaagtga
     3601 gtatatctac accttgataa ttcgagtaat tctctattcc aaaactatgt gaaacatcga
     3661 gtatcagtgt tgcgttatat ttatgcttta atgagactaa ttgaccaata tccacaacgt
     3721 cgccattcgt tgaaaataca ctatcagata tgattatttt tggtatattt tgattagggt
     3781 atttctctaa ccttttttct aaatcagcaa tatctaaatg cttatatatc actttttcta
     3841 aaccacttaa cttaataccg tcaataatac tcgcatgatt ttcttgatct gaaaacacga
     3901 cacaatttgt atttttgaaa atattaaata acgccaaatt agcatcataa ccactattta
     3961 agatagtaca tgcactatat ccgagccaac ctgctaacat tgtttcaatt tcttcataag
     4021 ctgtcgaact tccactaatt aatcttgaac ttgataagtg ataactatac ttccgcataa
     4081 atctttcgaa atcatcctta tcaaacgcta tttgacctaa tcctaaataa tcattagatg
     4141 tatagttcgt acatctctta ttttctactt caatatactg tctatctata taccctaccg
     4201 atttaagcga ccgatataac cctttctgtt gtaataaatc aatttgctct tgaaacttca
     4261 ttcttgtttt ccttattttc acaagtgtca taatcaattt caaagcctaa atcattaatc
     4321 atatcgtagt ctaattggtt cggttgccca ccagtaatta gataatcacc gacaaatatt
     4381 gaattcgccg cttttaatgc taatggctgt aacgaacgta agttgacctc tcttcctcca
     4441 gcaatacgaa tttctttcgt aggattgatt aatcggaata atgctacgat tcttaaacat
     4501 ttcattggtg ttaaatcatc catgcttcca aactttgtgc ctttgattgg atgcaaaaaa
     4561 ttaatcggaa tactgtcggc atccatttct tttaaagcaa atgccatatc aacaatatct
     4621 tgattagatt ctcccatacc acaaatcacg ccagaacatg gtgatatatt attcgctttc
     4681 attagttcta tcgtatctgt tctatcttta taactatgcg ttgtcacgac gttatcatgg
     4741 taattttcac ttgtattaat attgtggtta tatctgtcta caccagctga cttaagtttc
     4801 ttagcttgtt cgtcattcgt taatcctaaa catgcacaga tttttagttg cgggtgttga
     4861 gatttaatcg ttcttacagt attactaata tgatcaactt ctttatcgct cggtcctcta
     4921 ccactcataa caatacaata tgttccaata tgattatcat gtgccacctt tgctccatcg
     4981 ataatttgtt cctctggaat taaagcatat cgctgttttt gtttaatatc tcgtgattgt
     5041 ccacagtacc cacaattttc aggacatata ccacttttag catttaaaat catgtttaat
     5101 tttacttttt taccaaaata atgttttctt aaaatgtacg cctcatttaa taaatctaag
     5161 gtatcaatat tagtatcctc ataaattttc aatacagtct cttttgttaa ttgttcccct
     5221 tgtaatatgc gtttagccaa attcatatta acacttccta tctaaaatgt tatgtactgc
     5281 ctttttaaaa atacgtacca tcgttttcac ttctttattt gacatactta acactggcac
     5341 aaacgtaatg acattttcta aatttctaat cattagcccg ttttctttac aattacgtac
     5401 aatacgttca actttttcaa tttctaacgg cgttttacta tctttatctg taactaattc
     5461 aactccaaac attaagcctc gtcctcttac atcaccaaca tttcgatgac gcttcagcgc
     5521 atgtaactgt ttctcaagtg ttgatgatgt cgtttcaata tgtgacaata acttacgttt
     5581 ttcataaagt cttatatttt ctaatgcaac cgtacaaacg atttgatttc cggtgtatgt
     5641 atgaccatgg aaaaaggtat tcacaccatg cgaatcactt aaaaatgcat tgtatatttt
     5701 tttagatgtc aatgtagctg caagtggtaa gtagccacca gtaatcgcct tacctaaaca
     5761 cataatatcc ggttgaacat cttcatgatt gcatgcaaac atctttccag ttctcccaaa
     5821 accaactgct acctcatcac aaattaataa gacatcgtat tttttgcaca atttctcgac
     5881 ttctttcaaa aagcctttag gatgaacaaa caagcctgtc gcaccttgaa tcaacggttc
     5941 caatataaac cctgcgattt catcatttct ttcaattaga atttgctcta tattcgttaa
     6001 aatagctgtc atcatttcat tttcagtatc gtaattactg cgatagaaag atgggctttc
     6061 tacttgaata ttctcaaata ttaagtcttt aaatatttta tgaaaggtct tgataccacc
     6121 aacacttacc gcaccaatcg tatccccatg ataaccgtga tttagcgtta taaacttgtt
     6181 tttcttggca tatttttctc tatcaatatt tttccaatac tgatatgcca tctttattgc
     6241 gatttcaaca gacgcactgc ctgtatcaga ataaaatact tttcttagat tacttggcgt
     6301 gatttcgatt aatttttccg caagttctat tgacggaata tttgatgatc ctagcagcgt
     6361 agaatgggca attttattga gttgcttttt aattacctta tttaagtatt tgttattatg
     6421 accatgcaca ttgacccaca acgatgcata accatctaaa tatttattgc cattcgtatc
     6481 gtaaaggtaa ctaccctttc ctttttcaat gatgattgct tcttctttgc tatatacacc
     6541 catttgtgta aatggatgcc aaacatattc tgagtctttt tgtttaagtt gttgtgtgta
     6601 attcattttt aactcctatt aattgtttta aaaatgcttc tgagaaattt tcatacgtgg
     6661 catgttcttc aaatgtatag actgttttat ttgttaattt tccaatcgtc atttgattgt
     6721 ctttttcaat atagctgtct gtatagcgat tcattattaa aaaattactc gccgatacat
     6781 tctgattaac ataatcttgg tgaacaatgg catcgctaat agcacctaat tttgatggca
     6841 acacactgat gacacaatct gcacaatcat tgattagatc tttagtcatg tagaaatcat
     6901 ctgtaccttc atatattggt acggcaattc ccccagcacc ctcaattaag ataaaatcaa
     6961 attccttatc taaaactttt accttatcta atacacgctg cttatttaga aaaatttgat
     7021 ctgtcatttt aaatgcaagg tgtggtgata caggttgctt aaaagtataa agtgacgtta
     7081 tgtcatagct taaatcacat tcatttttaa atacttctaa atctggaaac gtcccgtctt
     7141 ggcgttcctc agtttgaaat ggtttaaaaa tacaaactcg atgaccacgt gttttcaaag
     7201 catggtataa atgctttgta acataggttt tgcctacatc agtattcgta cttgtaataa
     7261 aaatcctcat aaaaataacc acctcaatcc aaatgtaaac ttttataatt aataagttta
     7321 catttaaggc gcatgttttt caagaaaaca tgaatttaaa cttctcagta taactataaa
     7381 ttttggcaaa attttgacct tgctattgaa atgactaaga cttcttaagg aattcgggca
     7441 gatataaaca gcgattcgat aaaatatgtc agttaaaatt aataattgca ttatttctta
     7501 ataaaaagac atcctagctt taccatcttg tgaaacaatc cattaattgt aatattacgt
     7561 tgagcgcctt gttcatcaat gctataatta gttacataat aaattgaaca tctaaataca
     7621 ccaaatcccc tcactattgt cctagtgagg ggatttattt tgctgttgat tatctatcac
     7681 cctttttatt tccaaattct gattccatat taaactatta tttataattt ccagcctgct
     7741 gatttacttt ttattcttat aaagtcttga tacattcctg gttttcgaat taattcatca
     7801 tgactacctt tttgaattat ttcaccttca ttgagcacta taatctgatc tgcattttta
     7861 atagtttcaa gtttatgtgc aattgtaatt actgttttgc ctttacttaa ttcattaatt
     7921 gccgtttgaa tcagctgttc attttcaggg tcaatacttg cagttgcttc atctaaaata
     7981 attattggtg catcttttaa tatcgcccta gcaatagaaa tcctttgctt ttctccgcca
     8041 gataaattac tacctttttc atttagcatt gtttgatatc cctcaggtaa tgacatgatg
     8101 aagtcgtggc aacatgcttg cttcgctgca cgaataattt cttctttcgt tgcacctgga
     8161 ttgccaaaca atatgttatt ttcaatcgta tcattaaata aatacacctt ttgaaatact
     8221 gcactaattt tcgacattaa cgtacttaat gtcatatctt taatatcaac accatcgatg
     8281 cgaatatttc catcatcgat atcataaaag cgcaagagta agtgacataa tgtagatttc
     8341 ccacttcctg aaggtccaat tattgctgtt gatgtttgtg taggtatctc aaaattcaca
     8401 ttctttatca cttgtttatc atcatatgaa aagttcacgt tttgaaaagc gatattttca
     8461 tttttaatcg tcaaattttt tccattctca tccagttctg gagcattttt tatcttttca
     8521 atatcatcta tcgtcatatc tatcatttca agtacgtgtg cagcactatt aatattttca
     8581 acactatcaa atatcacaaa tgaaaaaatt gaaatcataa taagggtagg taaatcaata
     8641 ctatgattca tatataataa gcatgcaact aatacaatca ttattgaaac aacttttaaa
     8701 ctaagtaaat gcaataaatt gaatgggata tattgcattt ctatttttgt atttatacgt
     8761 ttactttcat tgactgcttg gttaaaactt ttaagactcg tattttcttt cgcgaatgat
     8821 tttattactt gaataccacg aataacctcc aatacctttt ccactaattg gttttgtaca
     8881 ttatgatacg ctggcgcatt ttgtcgactc tttctttcta ataattgaat cgcaaaaaat
     8941 gatagtaata cgccaatgca tgctaataat gatacttgcc acgaaactac aagtagagac
     9001 aatatgagta ctgtaattaa tatgtatcca ttaacaacaa cgtccaccat tttcatagca
     9061 aagttttcta aaaaggttaa atctgttgtt actattgttg ttaactcatt tgaatgatgc
     9121 gaattaaaat aacctaacct tacatttttc aatttatccc ctatatctaa acgttctttc
     9181 gcactcattt cataagcgat gctctcatgg cttttgcttt tgaaatatgc tgtaataaat
     9241 cgtccaatca ctaataaaac catgataatt acaacattca atatatcttt catataaata
     9301 ggtttatgag atagcacatt attaaatatt tttgcagcta aaaagatagg taacgcaata
     9361 aaaatagcat ttaaaaatga catgctaaat cctaaaatca ttcttgcttt atatggtctt
     9421 atccagttta aaattttaaa tgtaatttga aacatatttg acctcctttt ctcattagtt
     9481 taattatgtc cagtattgat tccccaatct tttgtatgca tgtgcgtatc ccacattttc
     9541 ttataattac cgtttaattt tagcaataag tgatgtgacc ctttttctaa aatttgttgt
     9601 ttacctaaaa caataatttg atctgcatgt tgaatcgtag ataaccgatg tgcaataaca
     9661 atcaatgttt tatcctgcgt caacacattt aatgcttctt gaattttttg ttcgttgtct
     9721 ggatcaacgt aaacagtcgc ttcatctaat acaatgatag gcgcatcttt taatatcatt
     9781 cttgcaatag tgactcgttg tttttcacca ccagacaatt tatctcccac tgtaccaaca
     9841 ttcgtatcat atccatctgg caacttttca ataaattcat gacattgtgc taacttggca
     9901 gctttttcaa ctgcctcatc cgtagcttct ggattgccaa gtttaatatt ttctttaaaa
     9961 gtaagattta ataaaaagtt atcttgtcca acaaaaccaa ctaaatcgtt aagttgtttc
    10021 gattcaatat cttttatatt tataccgcca atcgtaattt cacctgaagt cacatcccag
    10081 tatcgtgata taagcttggc aatggttgac ttaccgctac cagatgcccc gacgatagct
    10141 gtgaaattat tttctggtac tgtaaatgat aaatgcttaa agaccagatc gtccttatct
    10201 ttgttatatg aaaatccaac attattaaaa gcaatttcat aatgttgagg cttcttaaat
    10261 tttgttgaca atactaactc ttctagactt aatatttgat tcacttcagt taatgcgtat
    10321 tgtatagact ttaaatgatt tacataatta gtaaaattct taatcggtgc tactacacct
    10381 aaagataata cgatgcatag gaaaaattcc gcatagttta attggttgat agatatcaaa
    10441 tacatgccga ccggtaaaat ccctaaaaat gttgaaggta atacactagc tcctaaattc
    10501 atataccccc atgtattttt aaaccaattc aaagtgtgaa tcttataatt atctactgca
    10561 tctttatatt ttttatatga actttgagat tgattaaatg ttttaatcac ttcaatgcct
    10621 tcgataaact ctacaatcgc gctattcata taattattcg atttcatttg ttcagcatat
    10681 gtctcattaa atccagacat aactttttta aaagcgaaaa ttgaaattgg tatcgttact
    10741 aataaggcac tagccatacg ccaatcaatg agcattatgt ataaaaagat agcagctgac
    10801 aaaagtaagt ttcctataac ttcaggaatc atatgtgcta aaggtaattc tattgtttca
    10861 accttatcga caaatatatt ttttaattca cctattttct tagattccac tacgcctaaa
    10921 gggagacgca ttaatttttg agctaatttt ttacgaattt cagataaaat ttcatatgcc
    10981 gtaatatgtg atagcatcgt tgacgctcca aaacaacaca cttgtgaaat ataagcgatt
    11041 aaagcaataa agatataaac cataatcgaa ttaatcgtat atgtattgtt aatcatcatt
    11101 aaaataattt taaatactgc ccaataagga actaatccag aaaagacact gatgatagac
    11161 aacaaaattg ataacataat tttccattta ctatcagaag caaatccgct gagtatcttt
    11221 atccaatttg tctgattgtt catcgtatat gtaactcctt tcaattaata atctaaatta
    11281 agccgcttat attatttatt tcactggatg atatac
//
LOCUS       DAXWFK010000080        11273 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.080, whole genome shotgun sequence.
ACCESSION   DAXWFK010000080 DAXWFK010000000
VERSION     DAXWFK010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11273
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.080"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            208..1896
                     /gene="ilvD"
                     /locus_tag="ACGEXS_001603"
     CDS             208..1896
                     /gene="ilvD"
                     /locus_tag="ACGEXS_001603"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFF8012613.1"
                     /translation="MRSDMIKKGDHQAPARSLLHATGALKSPTDMNKPFVAICNSYID
                     IVPGHVHLRELADIAKEAIREAGAIPFEFNTIGVDDGIAMGHIGMRYSLPSREIIADA
                     AETVINAHWFDGVFYIPNCDKITPGMILAAMRTNVPAIFCSGGPMKAGLSAHGKALTL
                     SSMFEAVGAFKEGSISKEEFLDMEQNACPTCGSCAGMFTANSMNCLMEVLGLALPYNG
                     TALAVSDQRREMIRQAAFKLVENIKNDLKPRDIVTREAIDDAFALDMAMGGSTNTVLH
                     TLAIANEAGIDYDLERINAIAKRTPYLSKIAPSSSYSMHDVHEAGGVPAIINELMKKD
                     GTLHPDRITVTGKTLRENNEGKEIKNFDVIHPLDAPYDAQGGLSILFGNIAPKGAVIK
                     VGGVDPSIKTFTGKAICFNSHDEAVEAIDNRTVRAGHVVVIRYEGPKGGPGMPEMLAP
                     TSSIVGRGLGKDVALITDGRFSGATRGIAVGHISPEAASGGPIALIEDGDEITIDLTN
                     RTLNVNQPEDVLARRRESLTPFKAKVKTGYLARYTALVTSANTGGVMQVPENLI"
     gene            1924..3693
                     /gene="ilvB"
                     /locus_tag="ACGEXS_001604"
     CDS             1924..3693
                     /gene="ilvB"
                     /locus_tag="ACGEXS_001604"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HFF8012614.1"
                     /translation="MSKTQHEVNQNIDPLKMAESLEPEQLNEKTLNDMRSGSEVLVEA
                     LLKENVDYLFGYPGGAVLPLYDTFYDGKIKHILARHEQGAVHAAEGYARVSGKTGVVV
                     VTSGPGATNVMTGITDAHCDSLPLVVFTGQVATPGIGKDAFQEADILSMTSPITKQNY
                     QVKRVEDIPKIVHEAFHVANSGRKGPVVIDFPKDMGVLATNVDLCDEINIPGYEVVTE
                     PENKDIDTFISLLKEAKKPVVLAGAGINQSKSNQLLTQFVNKHQIPTVTTLLGLGAVP
                     YEDTLFLGMGGMHGSYASNMALTECDLLINLGSRFDDRLASKPDAFAPNAKIVHVDID
                     PSEINKVIHVDLGIIADCKRFLECLNDKNVETIEHSDWVKHCQNNKQKHPFKLGEEDQ
                     VFCKPQQTIEYIGKITNGEAIVTTDVGQHQMWAAQFYPFKNHGQWVTSGGLGTMGFGI
                     PSSIGAKLANPDKTVVCFVGDGGFQMTNQEMALLPEYGLDVKIVLINNGTLGMVKQWQ
                     DKFFNQRFSHSVFNGQPDFMKMAEAYGVKGFLIDKPEQLEEQLDAAFAYQGPALIEVR
                     ISPTEAVTPMVPSGKSNHEMEGL"
     gene            3693..3947
                     /locus_tag="ACGEXS_001605"
     CDS             3693..3947
                     /locus_tag="ACGEXS_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HFF8012615.1"
                     /translation="MTRILKLQVADQVSTLNRITSAFVRLQYNIDTLHVTHSEQPGIS
                     NMEIQVDIQDDTSLHILIKKLKQQINVLTVECYDLVDNEA"
     gene            4084..5088
                     /gene="ilvC"
                     /locus_tag="ACGEXS_001606"
     CDS             4084..5088
                     /gene="ilvC"
                     /locus_tag="ACGEXS_001606"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFF8012616.1"
                     /translation="MTTVYYDQDVKTDALQGKKIAVVGYGSQGHAHAQNLKDNGYDVV
                     IGIRPGRSFDKAKEDGFDVFPVAEAVKQADVIMVLLPDEIQGDVYKNEIEPNLEKHNA
                     LAFAHGFNIHFGVIQPPADVDVFLVAPKGPGHLVRRTFVEGSAVPSLFGIQQGASGQA
                     RNIALSYAKGIGATRAGVIETTFKEETETDLFGEQAVLCGGVSKLIQSGFETLVEAGY
                     QPELAYFEVLHEMKLIVDLMYEGGMENVRYSISNTAEFGDYVSGPRVITPDVKENMKA
                     VLTDIQNGNFSNRFIEDNKNGFKEFYKLREEQHGHQIEKVGRELREMMPFIKSKSIEK
                     "
     gene            5118..6647
                     /locus_tag="ACGEXS_001607"
     CDS             5118..6647
                     /locus_tag="ACGEXS_001607"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFF8012617.1"
                     /translation="MSSHIQIFDTTLRDGEQTPGVNFTFDERLRIALQLEKWGVDVIE
                     AGFPASSTGSFKSVQAIAQTLTTTAVCGLARCKKSDIDAVYEATKDAAKPVVHVFIAT
                     SPIHLEHKLKMSQEDVLASIKEHVTYAKQLFDVVQFSPEDATRTELPFLVKCVQTAVD
                     AGATVINIPDTVGYSYHDEYAHIFKTLTESVTSSNEIIYSAHCHDDLGMAVSNSLAAI
                     EGGARRIEGTVNGIGERAGNAALEEVALALYVRNDHYGAQTALNLEETKKTSDLISRY
                     AGIRVPRNKAIVGQNAFSHESGIHQDGVLKHRETYEIMTPQLVGVSTTELPLGKLSGK
                     HAFSEKLKALGYDIDKEAQIDLFKQFKAIADKKKSVSDRDIHAIIQGSEHEHQALYKL
                     ETLQLQYVSSGLQSAVVVVKDKEGHIYQDSSIGTGSIVAIYNAVDRIFQKETELIDYR
                     INSVTEGTDAQAEVHVNLLIEGKTVNGFGIDHDILQASCKAYVEAHAKFAAENVEKVG
                     N"
     gene            6650..7696
                     /gene="leuB"
                     /locus_tag="ACGEXS_001608"
     CDS             6650..7696
                     /gene="leuB"
                     /locus_tag="ACGEXS_001608"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFF8012618.1"
                     /translation="MTYNIVALPGDGIGPEILNGSLSLLEIISNKYNFNYQIEHHEFG
                     GASIDTFGEPLTEKTLNACKRADAILLGAIGGPKWTDPNNRPEQGLLKLRKSLNLFVN
                     IRPTTVVKGASSLSPLKEERVEGTDLVIVRELTSGIYFGEPRHFNNHEALDSLTYTRE
                     EIERIVHVAFKLAASRRGKLTSVDKENVLASSKLWRKVVNEVSQLYPEVTVNHLLVDA
                     CSMHLITNPKQFDVIVCENLFGDILSDEASVIPGSLGLSPSASFSNDGPRLYEPIHGS
                     APDIAGKNVANPFGMILSLAMCLRESLNQPDAADELEQHIYSMIEHGQTTADLGGKLN
                     TTDIFEILSQKLNH"
     gene            7710..9080
                     /gene="leuC"
                     /locus_tag="ACGEXS_001609"
     CDS             7710..9080
                     /gene="leuC"
                     /locus_tag="ACGEXS_001609"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFF8012619.1"
                     /translation="MGQTLFDKVWNRHVLYGKLGEPQLLYIDLHLIHEVTSPQAFEGL
                     RLQNRKLRRPDLTFATLDHNVPTIDIFNIKDEIANKQITTLQKNAIDFGVHIFDMGSD
                     EQGIVYMVGPETGLTQPGKTIVCGDSHTATHGAFGAIAFGIGTSEVEHVFATQTLWQT
                     KPKNLKIDINGTLPTGVYAKDIILHLIKTYGVDFGTGYALEFTGETIKNLSMDGRMTI
                     CNMAIEGGAKYGIIQPDDITFEYVKGRPFADNFAKSVDKWRELYSDDDAIFDRVIELD
                     VSTLEPQVTWGTNPEMGVNFSEPFPEINDINDQRAYDYMGLEPGQKAEDIDLGYVFLG
                     SCTNARLSDLIEASHIVKGNKVHPNITAIVVPGSRTVKKEAEKLGLDTIFKNAGFEWR
                     EPGCSMCLGMNPDQVPEGVHCASTSNRNFEGRQGKGARTHLVSPAMAAAAAIHGKFVD
                     VRKVVV"
     gene            9081..9653
                     /gene="leuD"
                     /locus_tag="ACGEXS_001610"
     CDS             9081..9653
                     /gene="leuD"
                     /locus_tag="ACGEXS_001610"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFF8012620.1"
                     /translation="MAAIKPITTYKGKIVPLFNDNIDTDQIIPKVHLKRISKSGFGPF
                     AFDEWRYLPDGSDNPDFNPNKPQYKGASILITGDNFGCGSSREHAAWALKDYGFHIII
                     AGSFSDIFYMNCTKNAMLPIVLEKSAREHLAQYVEIEVDLPNQTVSSPDKRFHFEIDE
                     TWKNKLVNGLDDIAITLQYESLIEKYEKSL"
     gene            9668..10936
                     /gene="ilvA"
                     /locus_tag="ACGEXS_001611"
     CDS             9668..10936
                     /gene="ilvA"
                     /locus_tag="ACGEXS_001611"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HFF8012621.1"
                     /translation="MTVKTTVSTKDIDEAFLRLKDIVKETPLQLDHYLSQKYDCKVYL
                     KREDLQWVRSFKLRGAYNAISVLSDEAKSKGITCASAGNHAQGVAYTAKKLNLNAVIF
                     MPVTTPLQKVNQVKFFGNSNVEVVLTGDTFDHCLAEALTYTSEHQMNFIDPFNNVHTI
                     SGQGTLAKEMLEQAKSDNVNFDYLFAAIGGGGLISGISTYFKTYSPTTKIIGVEPSGA
                     SSMYESVVVNNQVVTLPNIDKFVDGASVARVGDITFEIAKENVDDYVQVDEGAVCSTI
                     LDMYSKQAIVAEPAGALSVSALENYKDHIKGKTVVCVISGGNNDINRMKEIEERSLLY
                     EEMKHYFILNFPQRPGALREFVNDVLGPQDDITKFEYLKKSSQNTGTVIIGIQLKDHD
                     DLIQLKQRVNHFDPSNIYINENKMLYSLLI"
     gene            complement(11146..11260)
                     /gene="rrf"
                     /locus_tag="ACGEXS_001612"
     rRNA            complement(11146..11260)
                     /gene="rrf"
                     /locus_tag="ACGEXS_001612"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         3783 a         1869 c         2192 g         3429 t
ORIGIN      
        1 aacttcaaat attattattt tattatactc tttaggactc gaacgttagt aaatatttac
       61 taaacgcttt aagtcctatt tctgtttgaa tgggacttgt aaacgtccca ataatattgg
      121 gacgtttttt tatgttttat ctttcaatta cttattttta ttactataaa acatgattaa
      181 tcattaaaat ttacggggga atttactatg cgaagcgaca tgatcaaaaa aggagatcac
      241 caagcaccag caagaagtct tttacatgcc acgggcgcgc taaaaagtcc aactgatatg
      301 aacaaaccat ttgtagctat ttgtaactct tatattgata ttgttcctgg acatgttcac
      361 ttgagagagc ttgcagatat agctaaagaa gcaattagag aagccggtgc cattccattt
      421 gaattcaata caattggtgt tgatgatgga atagctatgg gacatatcgg aatgcgatat
      481 tctctaccat cacgtgaaat tattgcagat gcagctgaaa ctgtaattaa cgctcattgg
      541 tttgacggcg tattttacat tcctaattgt gacaagatta cacccggtat gattttagca
      601 gccatgagga caaacgtacc agctatcttt tgctctggtg gaccaatgaa agctggctta
      661 tctgcacatg gaaaagcatt aacactttca tcaatgtttg aagcagtcgg cgcatttaaa
      721 gaaggatcga tttctaaaga agaattttta gatatggaac aaaatgcctg ccctacttgt
      781 ggttcatgtg ctgggatgtt tactgcaaat tcaatgaact gtttgatgga agttttaggt
      841 ctagcattac catacaacgg tactgcactt gcagtcagtg atcagcgacg agaaatgatt
      901 cgccaagcag cttttaaatt agttgaaaat attaaaaatg atttaaaacc acgtgatatc
      961 gttactcgcg aagcaattga tgatgcattt gcacttgata tggctatggg tggttcaaca
     1021 aacacggtac tgcatacgtt agccattgcc aatgaagctg gtattgatta tgacttagag
     1081 cgcattaatg ctattgccaa acgcacgcca tatttatcaa aaatagcacc tagttcatcg
     1141 tattcaatgc atgatgtgca tgaagctggt ggcgtcccag caattattaa tgaattgatg
     1201 aagaaagatg gcacgttaca cccagataga atcacagtta ctggcaaaac gttacgtgaa
     1261 aataacgaag gcaaagaaat taagaacttt gatgtcattc accctcttga tgcaccatat
     1321 gatgcacaag gcggtttatc tatcttattt ggtaatatcg cccctaaagg cgcagttatt
     1381 aaagttggcg gcgttgatcc atctatcaaa acatttactg ggaaagcaat ttgtttcaat
     1441 tcgcatgatg aagctgttga agcaatagac aatcgtaccg ttcgtgcagg ccacgtcgtt
     1501 gtcattagat atgaaggacc taaaggtgga ccaggtatgc ctgaaatgtt agcacctact
     1561 tcctctattg ttggtcgcgg cttaggtaaa gatgttgcat taattactga tgggcgtttt
     1621 tccggtgcca caagaggtat tgcagttggt catatttccc ctgaagctgc atctggtgga
     1681 ccaattgcct taattgaaga tggtgatgag attactattg atttaacaaa tcgtacatta
     1741 aacgtaaacc agcctgaaga tgttctagcg cgtcgccgag aatctttaac accatttaaa
     1801 gcgaaagtaa aaacaggtta tctagctcgt tatactgccc tagtaactag cgcaaataca
     1861 ggtggcgtca tgcaagtccc tgagaattta atttaattta tttttatatt ggagatggtt
     1921 aaaatgtcta aaactcaaca tgaagtaaac caaaatattg accctttaaa aatggctgaa
     1981 tcacttgaac ctgaacaact aaatgaaaaa actttaaatg atatgcgttc aggatcagaa
     2041 gtgctagtag aagctctact taaagaaaat gtggattatt tattcggtta tcctggtggt
     2101 gccgtactac ctttatatga cacgttttat gatggtaaaa tcaaacatat tttagcaaga
     2161 cacgaacaag gtgctgttca tgctgcagaa ggttatgcac gtgtatctgg taaaactggc
     2221 gtcgttgtag ttacaagcgg tccaggtgca actaatgtaa tgacaggtat tacggatgca
     2281 cattgcgact ctttacctct agttgtattc actggacaag ttgctacacc aggcattggt
     2341 aaagatgcat tccaagaagc ggatattcta tctatgactt caccaattac aaaacaaaat
     2401 tatcaagtga aacgtgttga agatatccct aaaatcgtac acgaagcttt ccatgtagct
     2461 aattctggac gcaaaggtcc tgtagtgatt gattttccaa aagatatggg tgttttagct
     2521 acaaatgtgg atttatgcga cgaaatcaat attccaggtt atgaagttgt tacagaacca
     2581 gaaaataaag acattgacac tttcatctca cttttaaaag aagcgaaaaa gcctgtcgta
     2641 ttagccggcg caggtattaa tcaatcaaaa tcaaatcaat tattaacaca gtttgttaat
     2701 aaacatcaaa tcccaacagt tactacttta ctaggtctag gtgctgtacc ttatgaggat
     2761 acactatttt taggtatggg aggaatgcat ggttcttatg ctagtaacat ggcattaact
     2821 gagtgtgatt tactcattaa tttaggtagc cgcttcgatg atagattagc aagcaaacct
     2881 gatgcctttg cacctaacgc caaaattgta catgtagata ttgatccttc agaaatcaat
     2941 aaagttattc atgtagattt aggtattatt gcagactgta aaagattttt agaatgttta
     3001 aatgataaaa atgttgagac tatagaacac agtgactggg ttaaacattg tcaaaataat
     3061 aagcagaaac acccatttaa acttggtgaa gaagatcaag tattttgtaa gccacaacaa
     3121 acaatcgaat atatcggcaa aattacaaat ggtgaagcaa ttgttactac agacgtggga
     3181 caacatcaaa tgtgggcagc tcaattttat ccatttaaaa atcacggaca atgggttaca
     3241 agcggtggtt taggaacaat gggattcggt attccttcgt caattggtgc caaattagct
     3301 aatcctgata aaacagtcgt atgtttcgtc ggtgacggtg gtttccaaat gacaaaccaa
     3361 gaaatggcac ttttacccga atatggttta gatgtcaaaa tcgtactaat caataatgga
     3421 acattaggta tggttaaaca atggcaagat aagttcttta atcaacgctt ctcacactca
     3481 gtatttaatg gtcaacctga ttttatgaaa atggcagaag catatggcgt caaaggtttc
     3541 ttaatcgata agccagaaca actggaagaa caattagatg cagcgtttgc ttatcaagga
     3601 ccagctttaa ttgaggttcg tatttcccct actgaagctg taaccccaat ggttccgagt
     3661 ggcaaatcaa atcatgaaat ggagggctta taatgacaag aattcttaaa ttacaagttg
     3721 cggatcaagt cagcacgcta aatcgaatta caagtgcttt tgttcgccta caatataata
     3781 tcgatacatt acatgttaca cattctgaac aacctgggat ttctaacatg gaaattcaag
     3841 tcgatattca agatgataca tcacttcata tattaattaa aaaattaaaa caacaaatta
     3901 atgttttaac ggttgaatgc tacgaccttg ttgataacga agcttaattt taagacaaag
     3961 gcaatgatgc gctaattagt tatagatata tcataggctg ctagttaaca tctgccacta
     4021 ttacaaagtt atatttcaga attttcgaaa cacaaaatat ttaattattt ggaggaattt
     4081 attatgacaa cagtttatta tgatcaagat gtaaaaacgg acgctttaca aggcaaaaaa
     4141 attgcagtag taggttatgg atcacaaggt cacgcgcatg cacaaaactt aaaagacaat
     4201 ggatatgatg tagtcatcgg cattcgccca ggtcgttctt ttgacaaagc taaagaagat
     4261 ggatttgatg tgttccctgt tgcagaagca gttaagcaag ctgatgtaat tatggtgcta
     4321 ttacctgatg aaattcaagg tgatgtatac aaaaacgaaa ttgaaccaaa tttagaaaaa
     4381 cataatgcgc ttgcatttgc tcatggcttt aacattcatt ttggtgttat tcaaccacca
     4441 gctgatgttg atgtattttt agtagctcct aaaggaccgg gtcatttagt tagacgtaca
     4501 tttgttgaag gttctgctgt accatcacta tttggtattc aacaaggcgc ttcaggtcaa
     4561 gcacgtaata ttgctttaag ttatgcaaaa ggtattggtg caactcgtgc aggtgttatt
     4621 gaaacaacat ttaaagaaga aactgagaca gatttatttg gtgaacaagc agtactttgc
     4681 ggtggtgtat cgaaattaat tcaaagtggc tttgaaacat tagtagaagc gggttatcaa
     4741 ccagaattag cttattttga agtattacat gaaatgaaat taatcgttga tttgatgtat
     4801 gaaggcggta tggaaaatgt acgttactca atttcaaata ctgctgaatt tggtgactat
     4861 gtttcaggac cacgtgttat cacaccagat gttaaagaaa atatgaaagc tgtattaact
     4921 gatatccaaa atggtaactt cagtaatcgc tttatcgaag acaataaaaa tggattcaaa
     4981 gaattttata aattacgcga agaacaacat ggtcatcaaa ttgaaaaagt tggtcgtgaa
     5041 ttacgcgaaa tgatgccttt tattaaatct aaaagcattg aaaaataaga tagacctaca
     5101 atgaggagtt gttaaatatg agtagtcata ttcaaatttt tgatacgaca ctaagagacg
     5161 gtgaacaaac accaggagtg aattttactt ttgatgaacg cttgcgtatt gcattgcaat
     5221 tagaaaaatg gggtgtagat gttattgaag ctggatttcc tgcttcaagt acaggtagct
     5281 ttaaatctgt tcaagcaatt gcacaaacat taacaacaac ggctgtatgt ggtttagcta
     5341 gatgtaaaaa atctgacatc gatgctgtat atgaagcaac aaaagatgca gcgaagccgg
     5401 tcgtgcatgt ttttatagca acatcaccta ttcatcttga acataaactt aaaatgtctc
     5461 aagaagacgt tttagcatct attaaagaac atgtcacata cgcgaaacaa ttatttgacg
     5521 ttgttcaatt ttcacctgaa gatgcaacgc gtactgaatt accattctta gtgaaatgtg
     5581 tacaaactgc cgttgacgct ggagctacag ttattaatat tcctgataca gtcggctaca
     5641 gttaccatga tgaatatgca catattttca aaaccttaac agaatctgta acatcttcaa
     5701 atgaaattat ttatagtgct cattgccatg acgatttagg aatggctgtt tcaaatagtt
     5761 tagctgcaat tgaaggcggt gcgagacgaa ttgaaggcac tgtaaatggt attggtgaac
     5821 gagcaggtaa tgcagcactt gaagaagtcg cgcttgcact atacgttcga aatgatcatt
     5881 atggtgctca aactgctctt aatctcgaag aaactaaaaa aacatcggat ttaatttcaa
     5941 gatatgcagg tattcgagtg cctagaaata aagcaattgt tggccaaaat gcatttagtc
     6001 atgaatcagg tattcaccaa gatggcgtat taaaacatcg tgaaacatat gaaattatga
     6061 cacctcaact tgttggtgta agcacgactg aacttccatt aggaaaatta tctggtaaac
     6121 acgccttctc agagaagtta aaagcattag gttatgacat tgataaagaa gcgcaaatag
     6181 atttatttaa acaattcaag gccattgcgg acaaaaagaa atctgtttca gatagagata
     6241 ttcatgcgat tattcaaggt tctgagcatg agcatcaagc actttataaa ttggaaacac
     6301 tgcaactaca atatgtctct agcggccttc aaagtgctgt tgttgttgtt aaagataaag
     6361 agggtcatat ttaccaggat tcaagtattg gtactggttc aatcgtagca atttacaatg
     6421 cagttgatcg tattttccag aaagaaacag aattaattga ttatcgtatt aattctgtca
     6481 ctgaaggtac tgatgcccaa gcagaagtac atgtaaattt attgattgaa ggtaagactg
     6541 tcaatggctt tggtattgat catgatattt tacaagcctc ttgtaaagca tacgtagaag
     6601 cacatgctaa atttgcagct gaaaatgttg agaaggtagg taattaatta tgacttataa
     6661 cattgttgcc ctacctggtg atggaatcgg tccagaaatt ttgaacggat ctctatcatt
     6721 gcttgaaatt ataagtaata aatataactt taattatcaa atagagcacc acgaatttgg
     6781 tggtgcctct attgatacat tcggcgagcc tttaactgag aaaaccttaa atgcgtgtaa
     6841 aagagcagat gctattttac tgggtgcaat cggtggacct aaatggacag atcctaacaa
     6901 tcgaccagaa caaggattat taaaattgcg taaatcctta aatttatttg taaatatacg
     6961 ccccactacc gttgtcaaag gcgctagttc tttatcacct ttaaaggaag aacgcgttga
     7021 aggcacagat ttagttatag tccgtgaatt gacaagtggt atttattttg gagaacctag
     7081 acattttaat aatcacgagg ccttagattc tcttacttat acaagagaag aaatagaacg
     7141 cattgttcac gtagcattta aattggccgc ttcaagacga ggaaaactaa catcagttga
     7201 taaagaaaat gtattagctt ctagtaaatt gtggcgcaaa gtcgtaaatg aagtaagtca
     7261 attatatcca gaagtaacag taaatcactt acttgttgat gcttgtagta tgcatttaat
     7321 cacaaatcca aaacaatttg acgtcatcgt atgtgaaaac ttatttggcg atattttaag
     7381 tgatgaagct tcagtgattc ctggttcact tggtttatca ccttctgcta gttttagtaa
     7441 cgatggtcca agattgtatg agcctattca tggatcagca ccagatattg caggtaaaaa
     7501 cgttgccaat ccatttggaa tgattctatc tttagcgatg tgtttacgtg aaagcttaaa
     7561 tcaaccagat gctgcagatg aattagaaca acatatttat agcatgattg aacatgggca
     7621 aacgacagca gatttaggcg gcaaattgaa tactactgat attttcgaaa ttctatctca
     7681 aaaattgaat cactaagggg gagatgtaaa tgggtcaaac attatttgac aaggtgtgga
     7741 acagacatgt gttatacggg aaattgggcg aaccgcaact attatacatt gatttacacc
     7801 ttatacatga agttacttct cctcaagcat ttgaaggact taggcttcaa aacagaaaat
     7861 taagacgccc agatttaaca tttgcaacac tcgatcacaa tgttcctact attgatatat
     7921 tcaatattaa agatgaaatt gcaaacaaac aaatcacaac attacaaaaa aacgccatag
     7981 attttggggt gcatattttt gatatgggtt ctgatgaaca aggtattgtt tacatggtag
     8041 gacctgagac aggacttaca cagcctggca agacaatcgt ttgtggtgac tctcacacag
     8101 caacacatgg agcatttggt gctattgcat ttggaattgg aacaagtgaa gttgaacatg
     8161 ttttcgcaac tcaaacgcta tggcaaacaa aacccaaaaa cttaaaaatc gatattaatg
     8221 gtaccttacc aacaggcgtc tatgctaagg acattattct gcatttaatt aaaacgtatg
     8281 gtgttgactt tggtacaggc tatgctttgg aatttactgg cgaaacaatt aaaaaccttt
     8341 caatggatgg tcgaatgact atttgtaaca tggctatcga aggtggtgcc aaatacggca
     8401 taatccaacc tgatgatata acatttgaat atgttaaagg gagaccattt gccgataact
     8461 tcgctaaatc agttgataag tggcgtgagc tatattctga tgacgacgcg atatttgatc
     8521 gtgtaattga acttgatgtt tcaacattag aaccacaagt gacatgggga actaatcctg
     8581 aaatgggtgt taatttcagt gaaccattcc ctgaaatcaa tgatatcaac gatcaacgtg
     8641 cgtatgatta tatggggtta gaaccaggtc aaaaagctga agacatcgac ttagggtatg
     8701 tttttctcgg ttcatgtaca aatgctagac tatcagattt gattgaagct agtcatattg
     8761 ttaaaggaaa taaagttcat ccaaatatta cagctattgt cgtaccaggt tctcgtacag
     8821 taaaaaaaga agcagaaaaa ttaggtctag atactatctt taaaaatgca ggatttgaat
     8881 ggcgtgaacc aggatgttca atgtgtttag gcatgaatcc tgaccaagta cctgagggcg
     8941 tacattgtgc atctacaagt aatcgaaact ttgaaggacg acaaggcaaa ggtgcaagaa
     9001 cacatttagt atcccctgct atggcagcag cagcagctat tcatggtaaa tttgtggacg
     9061 taagaaaggt ggttgtttaa atggcagcaa tcaaacctat tacaacatat aaaggtaaaa
     9121 tagtccctct cttcaacgac aatatcgata cagaccaaat cattcctaag gtacacttaa
     9181 agcgtatttc aaaaagtggc tttggtccat ttgcttttga tgaatggcgg tacttacctg
     9241 atggttcaga taatcctgat ttcaatccta acaaaccaca atataaaggg gcttctattt
     9301 taattactgg agataatttt ggatgtggtt caagtcgtga acatgctgct tgggctctta
     9361 aggactatgg ttttcatatt attattgcag gaagtttcag tgacatattt tatatgaatt
     9421 gcactaaaaa tgcgatgttg cctatcgttt tagaaaaaag tgcccgtgaa catcttgcac
     9481 aatatgttga aattgaggtc gatttaccaa atcaaactgt gtcatcacca gacaagcgtt
     9541 tccattttga aattgatgaa acttggaaga ataaacttgt aaatggctta gatgacattg
     9601 caatcaccct acaatatgaa tcattaatag aaaaatatga aaaatcactt taagggagtt
     9661 gaatattatg acagtcaaaa caacagtttc tacgaaagat atcgatgagg catttttaag
     9721 acttaaagat attgtcaaag aaacaccttt acaattagac cattacttat ctcaaaagta
     9781 tgattgtaaa gtctatttaa aacgagaaga tttacaatgg gtacgttctt ttaaattaag
     9841 aggtgcttac aacgctattt ctgttttatc agatgaagct aaaagtaaag gtattacatg
     9901 tgcaagtgca ggtaatcatg ctcaaggtgt tgcctataca gctaaaaaac ttaatttaaa
     9961 cgctgttatc tttatgccag tcactacacc tttacaaaag gtaaatcaag taaagttctt
    10021 tggaaatagt aacgttgaag ttgtactcac tggtgataca tttgatcact gtttagctga
    10081 agctttaact tatacaagtg aacatcaaat gaactttata gatccattca ataatgttca
    10141 tacaatttct ggacaaggta cgcttgctaa agaaatgcta gaacaagcaa agtctgacaa
    10201 tgttaacttt gattatctat ttgccgcaat tggtggtggc ggtttaattt caggtattag
    10261 tacttacttt aaaacctatt cacctaccac gaaaattata ggtgttgaac cttcaggtgc
    10321 aagtagtatg tatgaatctg ttgtggtaaa taatcaggta gtcacattgc ctaatatcga
    10381 taaatttgtg gacggtgcat ctgtagctag agttggcgat attacatttg aaattgcaaa
    10441 agaaaatgta gatgattacg ttcaagtaga tgaaggtgca gtttgttcta cgattttaga
    10501 tatgtattca aaacaagcaa ttgtagcaga acctgctggc gcattaagtg taagtgcgct
    10561 tgaaaactat aaagatcata ttaaaggtaa aacagtggtt tgtgtcatta gtggtggtaa
    10621 taatgatatt aatcgaatga aagaaattga agaacgttca ttactatacg aagaaatgaa
    10681 gcattacttt atcttaaatt ttcctcaacg tccaggtgca ttgagagaat ttgtaaatga
    10741 cgtattagga cctcaagacg atattactaa atttgaatac ttaaaaaaat cttctcaaaa
    10801 tacaggtact gtcattattg gtattcaact taaagatcat gatgatttaa tacaactcaa
    10861 acaacgtgta aatcatttcg atccttccaa tatttatatt aatgaaaata agatgttata
    10921 ttcattgtta atttaacaca tagtaagaaa aacagtcata aattgatttc taattgaaat
    10981 catcttatga ctgcttttta ttatacttta catttctcgt ttcgtcagat tcaaacgttt
    11041 tcacttcgcc aagccatctt tctttgtgtt tgcttttatt ttgacgtttt agacataaaa
    11101 aaagagacct tgcggtctca atgcggctca tcgcatccac tttttgcctg gcaacgttct
    11161 actctagcgg aacgtaagtt cgactaccat cgacgctaag gagcttaact tctgtgttcg
    11221 gcatgggaac aggtgtgacc tccttgctat agtcaccaga catatgaatg taa
//
LOCUS       DAXWFK010000081        11270 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.081, whole genome shotgun sequence.
ACCESSION   DAXWFK010000081 DAXWFK010000000
VERSION     DAXWFK010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11270
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.081"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            35..988
                     /locus_tag="ACGEXS_001613"
     CDS             35..988
                     /locus_tag="ACGEXS_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012622.1"
                     /translation="MKKTLGCLLLIMLLVVAGCSFGGNHKLSSKKSEESKQETVKKES
                     EEEKDPDLEKYEEIEKKMKGIKDAPSLDKLDPLMTEKSFTNSKGIQGWKDYKELMGKV
                     ELADYRFTKDSKGSSIKDVDAFFKGKKGIKRKVIETHDDVKQVDYWYVDPDGKKIGNS
                     NTPVFYAEIMTKYKDGKLVYASVEPGSYVIHKDDAIKYDDYSKLKKLSQLTKLDHPKP
                     VPYSVAQIKSFGVPLTSVSFMTHGSKDTKDEVLPALAYFTFSPKNYEDKSNPDPKVLN
                     LVHMDFLNASSDFGNAHFVVLSKYIKEYESNYETSSDDSLK"
     gene            complement(1151..1354)
                     /locus_tag="ACGEXS_001614"
     CDS             complement(1151..1354)
                     /locus_tag="ACGEXS_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HFF8012623.1"
                     /translation="MQVNKVIYILLALFLGSFGIHKFYAGKNMQGILHLIFCWTGIPH
                     ILAIISAVITVFKPADEQGNVTL"
     gene            1653..2141
                     /locus_tag="ACGEXS_001615"
     CDS             1653..2141
                     /locus_tag="ACGEXS_001615"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFF8012624.1"
                     /translation="MAIKKLVPASHPILTKKAQAVKTFDDSLKRLLQDLEDTMYAQEA
                     AGLCAPQINQSLQVAIIDMEMEGLLQLVNPKIISQSNETITDLEGSITLPDVYGEVTR
                     SKMIVVESYDVNGNKVELTAHEDVARMILHIIDQMNGIPFTERADRILTDKEVEAYFI
                     ND"
     gene            2134..3069
                     /gene="fmt"
                     /locus_tag="ACGEXS_001616"
     CDS             2134..3069
                     /gene="fmt"
                     /locus_tag="ACGEXS_001616"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFF8012625.1"
                     /translation="MTKIIFMGTPDFSTTVLEMLIAEHDVIAVVTQPDRPVGRKRVMT
                     PPPVKKVAMKYDLPVYQPEKLSGSEELEQLLQLDVDLIVTAAFGQLLPESLLALPNLG
                     AINVHASLLPKYRGGAPIHQAIIDGEQETGITIMYMVKKLDAGNIISQQAIKIEENDN
                     VGTMHDKLSVLGADLLKETLPSIIEGTNESVPQDDTQATFASNIRREDERISWNKPGR
                     QVFNQIRGLSPWPVAYTTMDDTNLKIYDAELVETNKINEPGTIIETTKKAIIVATNDN
                     EAVAIKDMQLAGKKRMLAANYLSGAQNTLVGKKLI"
     gene            3066..4373
                     /gene="rsmB"
                     /locus_tag="ACGEXS_001617"
     CDS             3066..4373
                     /gene="rsmB"
                     /locus_tag="ACGEXS_001617"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFF8012626.1"
                     /translation="MIENVRSLAFDTIQDILNEGAYSNLRINEVLSENELNAMDKALF
                     TEIVYGTVKRKYTLDFYLKPFVKTKIKAWVRQLLWMSIYQYVYLDKVPNHAIINEAVE
                     IAKERGGYHNGNVVNGILRTMMRSDLPDFNEIADPKKRMAIEYSMPKWIIDHWATHYG
                     LEETETILQSFLETTSTTVRANLTRASLDDIIEKLQDEGYDVEKDHDLPYCLHIGGQP
                     IIHSRSFKDGFVSIQDKSSMFVAHIMNVDRHDHVLDACSAPGGKACHIAEVLMPEGQV
                     DASDIHDHKIDLINFNIKKLRLTNIKAFQHDATKPYDKTYDKILVDAPCSGLGVMRHK
                     PEIKYTQSKQHIESLVELQLEILENVKNNVKIGGEIIYSTCTIEQLENENVIYTFLKN
                     NKNFEFEPFQHPITGELVKTLQIMPQDFNSDGFFITKIKRKDN"
     gene            4376..5470
                     /gene="rlmN"
                     /locus_tag="ACGEXS_001618"
     CDS             4376..5470
                     /gene="rlmN"
                     /locus_tag="ACGEXS_001618"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002435140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFF8012627.1"
                     /translation="MITAEKKKKNKFLPNFDKQSIYSLRFDEMQNWLVEQGQQKFRAK
                     QIFEWLYQKRVDSIDEMTNLSKDLRQLLKDNFTVTTLTTVVKQESKDGTIKFLFELQD
                     GYTIETVLMRHDYGNSVCVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVSQVLTVQKA
                     LDATEERVSQIVIMGIGEPFENYDEMMDFLRIVNDDNSLNIGARHITVSTSGIIPRIY
                     DFADEDIQINFAVSLHAAKDEVRSRLMPINRAYNVEKLIEAIQYYQEKTNRRVTFEYG
                     LFGGVNDQLEHARELAHLIKGLNCHVNLIPVNHVPERNYVKTAKNDIFKFEKELKRLG
                     INATIRREQGSDIDAACGQLRAKERQVETR"
     gene            5477..6220
                     /locus_tag="ACGEXS_001619"
     CDS             5477..6220
                     /locus_tag="ACGEXS_001619"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase Stp1"
                     /protein_id="HFF8012628.1"
                     /translation="MLEAQFFTDTGQHRDKNEDAGGIFYNQTNQQLLVLCDGMGGHKA
                     GEVASKFVTDELKSRFEAENLIEQHQAENWLRNNIKDINFQLYHYAQENAEYKGMGTT
                     CVCALVFEKSVVIANVGDSRAYVINSRQIEQITSDHSFVNHLVLTGQITPEEAFTHPQ
                     RNIITKVMGTDKRVSPDLFIKRLNFYDYLLLNSDGLTDYVKDNEIKRLLVKEGTIEDH
                     GDQLMQLALDNHSKDNVTFILAAIEGDKV"
     gene            6217..8211
                     /gene="pknB"
                     /locus_tag="ACGEXS_001620"
     CDS             6217..8211
                     /gene="pknB"
                     /locus_tag="ACGEXS_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase Stk1"
                     /protein_id="HFF8012629.1"
                     /translation="MIGKIINERYKIVDKLGGGGMSTVYLAEDTILNIKVAIKAIFIP
                     PREKEETLKRFEREVHNSSQLSHQNIVSMIDVDEEDDCYYLVMEYIEGPTLSEYIESH
                     GPLSVDTAINFTNQILDGIKHAHDMRIVHRDIKPQNILIDSNKTLKIFDFGIAKALSE
                     TSLTQTNHVLGTVQYFSPEQAKGEATDECTDIYSIGIVLYEMLVGEPPFNGETAVSIA
                     IKHIQDSVPNVTTDVRKDIPQSLSNVILRATEKDKANRYKTIQEMKDDLSSVLHENRA
                     NEDVYELDKMKTIAVPLKKEDLAKHISEHKSNQPKRETTQVPIVNGPAHHQQFQKPEG
                     TVYEPKPKKKSTRKIVLLSLIFSLLMIALVSFVAMAMFGNKYEETPDVIGKSVKEAEQ
                     IFNKNNLKLGKISRSYSDKYPENEIIKTTPNTGERVERGDSVDVVISKGPEKVKMPNV
                     IGLPKEEALQKLKSLGLKDVTIEKVYNNQAPKGYIANQSVTANTEIAIHDSNIKLYES
                     LGIKQVYVEDFEHKSFSKAKKALEEKGFKVESKEEYSDDIDEGDVISQSPKGKSVDEG
                     STISFVVSKGKKSDSSDVKTTTESVDVPYTGKNDKSQKVKVYIKDKDNDGSTEKGSFD
                     ITSDQRIDIPLRIEKGKTASYIVKVDGKTVAEKEVSYDDV"
     gene            8439..9314
                     /gene="rsgA"
                     /locus_tag="ACGEXS_001621"
     CDS             8439..9314
                     /gene="rsgA"
                     /locus_tag="ACGEXS_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFF8012630.1"
                     /translation="MKTGRIVKSISGVYQVDVNGERFNTKPRGLFRKKKFSPVVGDIV
                     EFEVQNINEGYIHQVFERENELKRPPVSNIDTLVIVMSAVEPNFSTQLLDRFLVIAHS
                     YQLNARILVTKKDKTPIEKQFEINELLKIYENIGYETEFIGNDDDRKKFVEAWPAGLI
                     VLSGQSGVGKSTFLNHYRPELNLETNDISKSLNRGKHTTRHVELFERQNGYIADTPGF
                     SALDFDHIDKDEIKDYFLELNRYGETCKFRNCNHIKEPNCNVKHQLEIGNIAQFRYDH
                     YLQLFNEISNRKVRY"
     gene            9315..9959
                     /gene="rpe"
                     /locus_tag="ACGEXS_001622"
     CDS             9315..9959
                     /gene="rpe"
                     /locus_tag="ACGEXS_001622"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFF8012631.1"
                     /translation="MTKLYPSLLSVDFLDLQHELKRLEEAGVDGVHFDVMDGQFVPNI
                     SIGLPILDAVRKGTTLPIDVHLMIENPEKYIASFAEHGADMISIHVESTPHIHRAIQM
                     IKHLDKKAGVVINPGTPISQIEPILDIVDYVLVMTVNPGFGGQSFIDQCVEKIAGLNA
                     IKMERQLNFDIEVDGGVNTDTAKVCVENGATMLVTGSFFFKQEDYKKVTQALKG"
     gene            9966..10607
                     /locus_tag="ACGEXS_001623"
     CDS             9966..10607
                     /locus_tag="ACGEXS_001623"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFF8012632.1"
                     /translation="MHINLLCSDRHLPQDIWAKSNEGKWGGVDRGALILLKHQIIPFF
                     SVGDFDSVSKEERQLLTEQLQIKPVQAEKADTDLALAVDKAVALGFDSITIYGATGGR
                     LDHFFGAIQLLLKKAYYKHDVHIEVIDQQNKIELLPKGQHTVEKDKSYPYISFIPMTD
                     DVELSLAGFKYNLARQMLNIGSTLTISNEIESLQAKVTVHDGLILQIRSTDLN"
     gene            complement(10988..11176)
                     /gene="rpmB"
                     /locus_tag="ACGEXS_001624"
     CDS             complement(10988..11176)
                     /gene="rpmB"
                     /locus_tag="ACGEXS_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014614133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFF8012633.1"
                     /translation="MGKQCFVTGRKASTGNRRSHALNSTKRRWNANLQKVRILVDGKP
                     KKVWVSARALKSGKVTRV"
BASE COUNT         4193 a         1556 c         2103 g         3418 t
ORIGIN      
        1 gattcaaaca aaataactta agaggagaaa gtttatgaaa aagacactgg gatgtttact
       61 tttaattatg cttttagtcg tagcaggttg ttcttttggt gggaatcata aattatcatc
      121 aaagaaatca gaagaatcaa aacaagaaac tgtaaaaaaa gaatcggaag aagagaaaga
      181 tccagattta gagaaatatg aagaaataga gaagaaaatg aaaggaatta aagatgcgcc
      241 atctcttgat aagttggatc cattaatgac agaaaagtcg tttacgaata gtaaagggat
      301 tcaaggatgg aaagattaca aagaattaat gggtaaagtg gaacttgcag attatagatt
      361 tactaaagat tcaaaaggat cttcaataaa agatgttgat gcattcttta aaggtaagaa
      421 aggtataaaa aggaaagtga ttgaaacaca cgatgatgta aaacaagttg attattggta
      481 tgtagatcca gatggaaaga aaattggcaa ttcaaacaca cctgtttttt acgcagaaat
      541 tatgacaaaa tataaagatg gaaagttagt ttatgcatca gtcgaaccag gatcttacgt
      601 aatacataaa gatgatgcaa ttaaatatga cgattattct aagttaaaaa aattaagtca
      661 gctaactaaa cttgatcatc caaaaccagt tccatatagc gtagctcaaa tcaaatcttt
      721 cggagtacct ttaacaagcg tttcatttat gacacatgga tcaaaggata ctaaagatga
      781 agtgttgccg gcattggcct atttcacttt ttcaccaaaa aattatgaag acaagtctaa
      841 tccagatcca aaagttttaa atttagtaca tatggatttc ttaaatgcat ctagtgattt
      901 tggtaacgca cattttgttg ttttaagtaa atatattaaa gagtatgaat caaactatga
      961 aacatcgtca gatgattctt taaaatagta tttactgtgt gaaaaataaa tagtgtacta
     1021 cattaaataa tcgcaataat aatcccgata aacaatcagc attactgctt atcacataga
     1081 gttcgtaata actataactc tatgattcgc aaataataaa tgattgtcat cgggatttat
     1141 ttttatcaat ttataaagtg acattacctt gttcatcagc aggtttgaaa acagtaatca
     1201 ctgcactaat aattgctaaa atgtgtggga tacctgtcca acagaatatt aagtgtagaa
     1261 taccttgcat attcttgccg gcataaaatt tatgaatacc aaaactacct aagaacaatg
     1321 ctaataaaat ataaataact ttgtttactt gcatttcttt ccctccagtt gaattgctta
     1381 taatgacatt agcttctctt tttattatac ccacttttag ttcaaacatt ctagtttaag
     1441 cattcccaat catctaaatt tcagttattc aatccttaca ataaatttag gattacattt
     1501 cagttgcatt gtattatttt acgtgtgaaa tatacgtaat gaatcacatg acaatcttca
     1561 aattgaaaaa tatacattct atgatgtaag gtcgcatttt taatatattt acgttataat
     1621 agtttgatga attttattga ggagtaagag ttatggcgat taaaaagtta gtaccagcat
     1681 cgcatcctat tttaacgaaa aaagcgcaag cagttaaaac atttgatgat tcgttaaaaa
     1741 gattattaca agatttagaa gatacaatgt atgcacaaga agctgctggc ttatgtgcac
     1801 ctcaaattaa tcagtcattg caagtggcaa ttattgatat ggaaatggaa ggattattac
     1861 aacttgttaa tccgaaaatt attagtcaat caaatgaaac aataacagac ttagaaggtt
     1921 caattacatt gccagatgtt tacggcgaag tgacaagaag taaaatgata gttgtcgaaa
     1981 gttatgacgt caatgggaac aaagttgaac taactgcaca tgaagatgta gcaagaatga
     2041 ttttgcatat tatagatcaa atgaacggta tcccttttac agaacgtgcg gaccgtattt
     2101 taacagataa agaagtggag gcatatttta taaatgacta aaataatatt tatgggtaca
     2161 ccagactttt caacaactgt tttagaaatg cttattgcag aacatgatgt cattgcagtc
     2221 gtaacgcaac cagatcgacc tgttggacgt aaacgtgtta tgacaccacc accagttaaa
     2281 aaagttgcaa tgaaatatga tttacctgta tatcaacctg aaaaattaag tggatcagaa
     2341 gaattagaac aattgcttca attagatgta gatttaattg taactgctgc ttttggacaa
     2401 ttattacctg aatcattgtt ggcattacca aatcttgggg caattaatgt acatgcatca
     2461 ttgttaccga agtatagagg tggtgcacca attcatcagg caattatcga tggtgaacaa
     2521 gaaaccggca taacaattat gtatatggtt aaaaaattag atgcgggtaa tattatttcg
     2581 caacaagcaa ttaaaataga agaaaatgat aatgtcggta cgatgcatga taaattaagt
     2641 gtattagggg cagatttatt aaaagaaact ttaccatcta ttatagaggg cacaaatgaa
     2701 agcgtacctc aagatgatac gcaagcaaca tttgcttcca atattcgacg cgaagatgag
     2761 cgaattagct ggaataaacc aggaagacaa gtgtttaatc aaattcgtgg attatcacca
     2821 tggccagttg cttatacaac tatggatgac actaacttga aaatatacga tgctgaactc
     2881 gttgagacta ataagataaa cgagcctgga accattatag aaacgactaa aaaagccatt
     2941 attgttgcta caaatgataa tgaagctgtt gcaattaaag atatgcaatt agctgggaaa
     3001 aagagaatgt tagctgccaa ttatttaagt ggtgcgcaaa acacactagt agggaagaaa
     3061 cttatatgat agaaaacgtg agaagtcttg cttttgacac gattcaagat atattaaatg
     3121 aaggtgcgta tagtaacttg cgtatcaatg aagtgttgtc agaaaatgaa ttaaatgcaa
     3181 tggataaggc tttatttaca gaaattgtct acggaaccgt taaaagaaaa tatacgttag
     3241 atttttattt aaagcctttt gtgaaaacaa agattaaggc atgggttagg caattattat
     3301 ggatgagtat ttatcaatat gtttatttag ataaagttcc aaatcatgcc attattaatg
     3361 aagcagttga aatagcaaaa gaacgcggtg gctatcataa tggtaatgtc gtaaatggta
     3421 ttttacgtac aatgatgcgt agtgacttac ctgattttaa tgaaattgca gatcctaaaa
     3481 aaagaatggc aatcgagtat agtatgccga agtggattat agatcattgg gcaacacatt
     3541 atggtctcga agaaactgaa acaattttac agtcattttt agaaacgaca tcaacaactg
     3601 tgcgtgccaa cctgacgcga gcatcattag atgatattat tgaaaagttg caagacgaag
     3661 gttatgacgt tgaaaaagat catgacttac cttattgtct ccatatagga ggacaaccaa
     3721 ttattcattc tcgttcattt aaagatggat tcgtttcaat tcaagataaa agctcaatgt
     3781 ttgttgcaca cattatgaat gtagaccgac atgatcacgt attagatgca tgtagtgcac
     3841 ctggcggtaa agcttgtcac attgctgaag ttttaatgcc agaagggcaa gttgacgctt
     3901 cagatataca tgatcacaaa atagacttaa ttaattttaa tataaaaaaa ttacgattaa
     3961 caaatattaa agcttttcaa catgatgcga caaaacctta tgataaaaca tacgataaga
     4021 tacttgttga tgcaccatgt agcggattag gtgtaatgag acataagccg gagattaagt
     4081 atactcaaag caaacaacat attgagtcac tagttgaatt acagcttgaa atattggaaa
     4141 atgtaaaaaa caatgtaaaa ataggtggcg aaatcatcta ttcaacatgt acaattgagc
     4201 aactagaaaa tgaaaacgtg atttatacgt ttttgaaaaa taataaaaac ttcgaatttg
     4261 aaccgtttca acatccgata actggagagt tggtcaaaac gttacaaatc atgccgcaag
     4321 actttaattc agatggattc tttatcacta agataaaaag aaaggacaat taggaatgat
     4381 aactgctgaa aagaaaaaga agaataaatt tcttccaaat tttgacaagc aatcaatata
     4441 ttcattgcga tttgacgaaa tgcaaaactg gctcgttgaa caagggcaac aaaaatttcg
     4501 agcgaaacag atttttgaat ggttatatca aaaaagagta gattcgattg atgaaatgac
     4561 gaacttatcg aaagacttac gacagctttt aaaagataac tttactgtta caactttaac
     4621 aactgtagta aaacaagaaa gtaaagacgg tacaattaaa ttcttatttg aattacaaga
     4681 tggctataca attgaaactg ttttaatgag acatgattat ggaaattcag tatgtgtaac
     4741 gacacaagta ggttgtcgca tcggatgtac gttttgtgct tctacacttg gcggcttaaa
     4801 aagaaacctt gaagctggcg aaattgtttc acaagtttta acagttcaaa aagcccttga
     4861 tgctacagaa gagcgcgtat ctcaaattgt cataatgggt atcggtgaac catttgaaaa
     4921 ttatgatgaa atgatggact ttttaagaat cgtcaatgat gataacagtt taaatattgg
     4981 tgcacgtcac attacagtat caacatcagg tatcattcct agaatatatg actttgcgga
     5041 tgaagatatt caaattaatt ttgctgtaag cttacacgcc gcaaaagatg aagtgcgatc
     5101 acgcttgatg ccaattaacc gtgcatataa tgttgagaag ttaatcgaag caattcaata
     5161 ttatcaagaa aaaacaaatc gtcgtgttac ttttgaatat ggtctgtttg gtggtgtgaa
     5221 tgaccaacta gaacatgcaa gagaattagc acatttaata aaaggcttaa actgccatgt
     5281 taacttaatt cctgtcaacc atgttccaga aagaaattat gtgaaaacgg ctaaaaatga
     5341 tatctttaaa tttgaaaaag aattaaagag actaggaatt aatgccacaa tacgtcgtga
     5401 acaaggttcg gatattgacg cagcttgtgg tcaattaaga gcaaaggaac gacaagtaga
     5461 aacgaggtaa agacaaatgc tagaggcaca attttttact gatactggac aacatagaga
     5521 taagaatgaa gatgcgggtg gtatttttta taatcaaact aatcaacaac ttttagttct
     5581 gtgtgatggt atgggtggcc ataaagcagg agaagttgca agtaaatttg ttacagatga
     5641 gttgaaatcc cgttttgaag cggaaaatct tatagaacaa catcaagctg aaaattggtt
     5701 gcgtaataat ataaaagata taaattttca gttatatcac tatgcacaag aaaatgcaga
     5761 atataaaggt atgggtacaa catgtgtttg tgcacttgtt tttgaaaaat cagttgtgat
     5821 agcaaatgtc ggtgattcta gagcctatgt tattaatagt cgacaaattg aacaaattac
     5881 tagtgatcac tcatttgtta atcatcttgt tttaacgggt caaattacgc cggaagaagc
     5941 atttacacat ccacaacgta atattattac gaaggtgatg ggcacagata aacgtgtgag
     6001 tccagatttg tttattaagc gattaaattt ttatgattat ttattattaa attcagatgg
     6061 attaactgat tatgttaaag acaatgaaat taagcgtttg ttagtaaaag aaggtacaat
     6121 agaagatcat ggtgatcaat taatgcaatt ggcattagat aaccattcga aagataacgt
     6181 tactttcata ctcgcggcta ttgaaggtga taaagtatga taggtaaaat aataaatgaa
     6241 cgatataaaa ttgtagataa gcttggcggc ggtggcatga gtaccgttta tcttgctgaa
     6301 gatacgatac ttaacattaa agttgcaatt aaggcgattt ttataccacc tagagaaaaa
     6361 gaagaaacat taaaacgttt tgaacgagaa gtacataact catcacagct atcacatcaa
     6421 aatatagtaa gtatgatcga tgttgatgaa gaagatgact gttactactt agtaatggaa
     6481 tatatcgaag gtccgacttt gtctgagtat attgaaagtc atgggccatt aagtgttgac
     6541 acagcgatta attttacgaa tcaaatattg gatggcatta aacatgcgca tgatatgcgt
     6601 attgtacata gagatattaa gccacaaaat atattaattg acagcaataa aacgttgaaa
     6661 atatttgatt ttggaattgc taaagcttta agtgagacgt ctttaactca gactaatcat
     6721 gtgttaggta ctgtgcagta cttttcgcca gaacaagcaa aaggtgaggc aacggatgaa
     6781 tgtacagata tttattctat aggtattgtg ttatatgaaa tgcttgttgg tgaaccaccc
     6841 tttaatggag aaactgcagt tagcattgcg attaaacata ttcaggattc tgtgccaaat
     6901 gtgacaacag atgtacgtaa ggatattccg caatctttaa gtaatgtcat tttacgcgct
     6961 acagaaaaag acaaagcgaa tcgttacaaa acaattcaag aaatgaaaga tgatttgagt
     7021 agtgttttac atgaaaatcg agcgaatgaa gatgtctatg aactcgataa aatgaaaacg
     7081 atagcggtac ctttgaaaaa agaagatcta gcaaagcata ttagtgaaca taagtcgaat
     7141 caacctaaac gtgaaacgac gcaagtacct attgtaaatg ggcctgctca tcatcagcaa
     7201 ttccaaaagc cagaaggtac ggtgtacgaa ccaaaaccta aaaagaaatc aacacgaaag
     7261 attgtgctct tatcactaat cttttcgttg ttaatgattg cacttgtttc ttttgtggca
     7321 atggcaatgt ttggtaataa atacgaagag acacctgatg taatcgggaa atctgtaaaa
     7381 gaagcagagc aaatattcaa taaaaacaac ctgaaattgg gtaaaatttc tagaagttat
     7441 agtgataaat atcctgaaaa tgaaattatt aagacaactc ctaatactgg tgaacgtgtt
     7501 gaacgtggtg acagtgttga tgttgttata tcaaagggcc ctgaaaaggt taaaatgcca
     7561 aatgtcattg gtttacctaa ggaggaagcc ttgcagaaat taaaatcgtt aggtcttaaa
     7621 gatgttacga ttgaaaaagt atataataat caagcgccaa aaggatacat tgcaaatcaa
     7681 agtgtaaccg caaatactga aatcgctatt catgattcta atattaaact atatgaatct
     7741 ttaggcatta agcaagttta tgtagaagac tttgaacata aatcctttag caaagctaaa
     7801 aaagccttag aagaaaaagg gtttaaagtt gaaagtaagg aagagtatag tgacgatatt
     7861 gatgagggtg atgtgatttc tcaatctcct aaaggaaaat cagtagatga ggggtcaacg
     7921 atttcatttg ttgtttctaa aggtaaaaaa agtgactcat cagatgtcaa aacgacaact
     7981 gaatcggtag atgtaccata cactggtaaa aatgataagt cacaaaaagt taaagtttat
     8041 attaaagata aagataatga cggttcaact gaaaaaggta gtttcgatat tactagtgat
     8101 caacgtatag acattccttt aagaattgaa aaaggaaaaa cagcaagtta tattgttaaa
     8161 gttgacggta aaactgtagc tgaaaaagaa gtcagctatg atgatgtata aatataattg
     8221 aagtaaatgt accgaggttt ctatttggaa gtctcggtat ttttatgttg agattgcggt
     8281 agttttaaaa tgcttcatgt catcatatac aggtgagcat tgtctatcaa ttctgtatca
     8341 ctctcacaat atttatatca tttgaaatga tcatttatat attaattttc cagtctttga
     8401 tataataaat gcagtaaaat taatcgagag gtgccatttt gaagacaggt cgaatagtga
     8461 aatcaattag tggggtatat caagtagacg ttaatggcga acgtttcaat acaaaaccac
     8521 gaggattatt tagaaagaaa aaattttcac cggtagttgg tgatatagtg gaatttgaag
     8581 ttcaaaacat taacgaaggt tatattcatc aagtgtttga gcgggaaaat gagttgaaaa
     8641 gaccacctgt aagtaatata gatacactag taattgtaat gagtgctgtc gagccaaatt
     8701 tttcaacgca attattagat cgatttttag ttattgcaca ttcgtatcag ttaaatgcga
     8761 gaattttggt gactaaaaaa gataaaacac caattgaaaa gcagttcgaa ataaatgagt
     8821 tgttgaaaat atatgaaaat attggctatg agactgaatt tattggaaat gatgatgatc
     8881 gaaaaaaatt tgtagaagct tggccagctg gacttatagt acttagtggt caatcaggtg
     8941 tcggtaagtc cactttctta aatcattatc gtccagaact taatcttgag acaaatgata
     9001 tatcaaaatc attaaatcga ggaaagcata ctacaagaca tgtcgaacta ttcgaacgtc
     9061 aaaacggtta tattgcagac acacctggat tcagtgcttt agattttgat catatagata
     9121 aagatgaaat aaaagattat tttcttgaat taaatcgata tggtgaaaca tgtaagttta
     9181 ggaattgtaa tcatatcaaa gaacctaatt gtaatgttaa gcatcaatta gagataggga
     9241 atattgcgca atttagatac gaccattatt tacaactatt taatgaaatt tcaaatagaa
     9301 aggttagata ttaaatgaca aaactatatc catcattatt atctgttgat tttttggatt
     9361 tacaacatga attaaaacga cttgaagaag caggcgtcga cggagttcat tttgatgtta
     9421 tggatggtca atttgtgcct aatatatcta ttggtttacc aatattagat gcagtaagaa
     9481 aaggcacaac attacctata gacgtacatt tgatgattga aaatccagaa aagtatattg
     9541 catcatttgc agaacatggt gccgatatga tttcaattca tgtcgaatca acgcctcata
     9601 ttcatcgtgc tattcaaatg attaaacatt tagataaaaa agctggtgta gtaattaatc
     9661 ctggtacacc aatatcacaa attgaaccta ttttagacat tgttgattat gtactagtga
     9721 tgacagttaa cccagggttt ggtggtcaat catttattga tcaatgcgta gaaaaaatag
     9781 cgggtcttaa tgctattaaa atggaacgtc aattaaactt tgatattgaa gttgatggag
     9841 gcgtaaatac cgatacagcg aaagtttgtg ttgaaaatgg tgctacaatg ctagtaacag
     9901 gttcattttt ctttaaacaa gaggattata aaaaagtcac acaagcattg aaaggttgag
     9961 tgtatatgca tataaattta ttatgttctg atcgacactt gccgcaagat atttgggcca
    10021 aaagtaatga aggtaaatgg ggcggcgttg atagaggtgc tttgatttta ttgaagcatc
    10081 aaattatccc ttttttctca gtgggagact ttgattcagt cagtaaagaa gaacgccaac
    10141 ttctaacaga acagttacaa atcaaaccag ttcaagctga aaaagctgat acggatttag
    10201 ctttagcggt tgataaagct gttgcacttg gatttgatag tattacaatt tatggtgcaa
    10261 caggcggacg attagatcac ttttttgggg caattcagtt attattgaaa aaagcatatt
    10321 ataaacatga tgttcatata gaagttatcg atcaacaaaa taaaattgaa ttattgccta
    10381 aaggtcaaca tacagttgaa aaagataaga gttatccgta catttcattt ataccgatga
    10441 ctgatgatgt agaactttct ctagcaggtt ttaaatataa tttagctaga caaatgctta
    10501 atataggttc tactttaact atttcaaatg aaattgagtc tttgcaagcg aaagtaactg
    10561 tacatgatgg gttgattttg caaattagaa gtacagattt aaattaagta tattcaaatt
    10621 tgtgaggatt tttaataaag tgcatgagtt atttaataaa gtgacgttta tagatagaat
    10681 ttataacata ataatagatg aatcacgctg taattaatga agattatggc gtgatttatt
    10741 attgaaagga aaattgtagg tcctagtaca ttgatttatg tgccaaaatc gatattgaac
    10801 ccataagtcc aatttgacag agaaccatta ttaaacgtat taaccaaatt atattgatag
    10861 ttcgaatttc actatgaacc attatgtatt gtttggtaac aaaaaaagaa ctaagaggtt
    10921 gagctcttag ttcttaagta aatgtgtcct ccacataagg tcacattttt tatgtgcaat
    10981 atatttatta aactctagtt actttaccag attttaaagc acgtgcagaa acccaaactt
    11041 ttttaggttt accgtcaact aggattctaa ctttttgaag gttagcgttc catctacgtt
    11101 tagtagagtt taaagcgtgt gaacgtctgt taccagtcga agctttacga cctgttacga
    11161 aacattgttt acccatatga gtacctcctt taataaatat aaatacacat aactacttat
    11221 atacttaatt aagatagcat agtttcattt gaaaaacaat gaataatttt
//
LOCUS       DAXWFK010000082        11212 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.082, whole genome shotgun sequence.
ACCESSION   DAXWFK010000082 DAXWFK010000000
VERSION     DAXWFK010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11212
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.082"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            26..2731
                     /gene="acnA"
                     /locus_tag="ACGEXS_001625"
     CDS             26..2731
                     /gene="acnA"
                     /locus_tag="ACGEXS_001625"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFF8012634.1"
                     /translation="MAANFKEQSKKHFDLNGQSYTYYDLKAVEEQGITKVSNLPYSIR
                     VLLESLLRQEDDFVITDDHIKALSQFGKDGNEGEVPFKPSRVILQDFTGVPAVVDLAS
                     LRKAMDDVGGDITKINPEVPVDLVIDHSVQVDSYANPEALERNMKLEFERNYERYQFL
                     NWATKAFDNYNAVPPATGIVHQVNLEYLASVVHVRDVDGEKTAFPDTLVGTDSHTTMI
                     NGIGVLGWGVGGIEAEAGMLGQPSYFPIPEVIGVRLVNSLPQGATATDLALRVTQELR
                     KKGVVGKFVEFFGPGVQHLPLADRATIANMAPEYGATCGFFPVDDESLKYMKLTGRSD
                     EHIALVKEYLKQNHMFFDVEKEDPNYTDVIELDLSTVEASLSGPKRPQDLIFLSDMKS
                     SFENSVTAPAGNQGHGLDKSEFDKKAEINFKDGSKATMKTGDIAIAAITSCTNTSNPY
                     VMLGAGLVAKKAVEKGLKVPEYVKTSLAPGSKVVTGYLRDAGLQPYLDDLGFNLVGYG
                     CTTCIGNSGPLLPEIEKAIADEDLLVTSVLSGNRNFEGRIHPLVKANYLASPQLVVAY
                     ALAGTVDIDLQNEPIGKGNDGEDVYLKDIWPSIKEVSDTVDSVVTPELFIEEYNNVYN
                     NNELWNEIDVTDQPLYDFDPNSTYIQNPSFFQGLSKEPGTIVPLNGLRVMGKFGDSVT
                     TDHISPAGAIGKDTPAGKYLQDHQVPIREFNSYGSRRGNHEVMVRGTFANIRIKNQLA
                     PGTEGGFTTYWPTNEVMPIFDAAMKYKEDGTGLVVLAGNDYGMGSSRDWAAKGTNLLG
                     VKTVIAQSYERIHRSNLVMMGVLPLEFKKGESADSLGLDGTEEISVNIDENVQPHDYV
                     KVTAKKQDGDLVEFDAMVRFDSLVEMDYYRHGGILQMVLRNKLAQ"
     gene            2911..3378
                     /locus_tag="ACGEXS_001626"
     CDS             2911..3378
                     /locus_tag="ACGEXS_001626"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFF8012635.1"
                     /translation="MIYSITEIEARYAETDKMGVIYHGNYATWFEVARLDYISKLGFS
                     YADMEKQGIISPVTDLNVNYKKSIFYPEKVKVKTWVEKYSRLRSVYKYEIFNEKGELA
                     TTGSTELICIKEDTFKPIRLDRYFPDWHEAYSKVQTLNNEGKTVEIMDGIDSL"
     gene            complement(3585..3881)
                     /locus_tag="ACGEXS_001627"
     CDS             complement(3585..3881)
                     /locus_tag="ACGEXS_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFF8012636.1"
                     /translation="MQIELTDAAVTWFKNELELPENNKVLVFFVRYGGEFQLKQGFSP
                     AFTVEPKEDVDIGYEQQYDDLNVVVAEKDLWYFEDDHIIVNVVDHEDEISYSTK"
     gene            complement(4257..4865)
                     /gene="plsY"
                     /locus_tag="ACGEXS_001628"
     CDS             complement(4257..4865)
                     /gene="plsY"
                     /locus_tag="ACGEXS_001628"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFF8012637.1"
                     /translation="MMIIVMLLLSYLIGAFPSGFVIGKLFFKKDIRQFGSGNTGATNS
                     FRVLGRPAGFLVTFLDIFKGFITVFFPLWLQVHADGPISTFFTNGLIVGLFAILGHVY
                     PVYLKFQGGKAVATSAGVVLGVNPILLLILAIIFFIVLKIFKYVSLASIVAAICCVIG
                     SLIIQDYILLVVSFLVSIILIIRHRSNIARIFRGEEPKIKWM"
     gene            5071..7062
                     /gene="parE"
                     /locus_tag="ACGEXS_001629"
     CDS             5071..7062
                     /gene="parE"
                     /locus_tag="ACGEXS_001629"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFF8012638.1"
                     /translation="MNKQNNYSDDSIQVLEGLEAVRKRPGMYIGSTDKRGLHHLVYEI
                     VDNSVDEVLNGYGNEIDVTINKDGSISIEDNGRGMPTGIHKSGKPTVEVIFTVLHAGG
                     KFGQGGYKTSGGLHGVGASVVNALSEWLEVEIHRDGNIYHQSFKNGGSPSSGLVKKGK
                     TKKTGTKVTFKPDDTIFKASTSFNFDVLSERLQESAFLLKNLKITLNDLRSGKERQEH
                     YHYEEGIKEFVSYVNEGKEVLHDVATFSGEANGIEVDVAFQYNDQYSESILSFVNNVR
                     TKDGGTHEVGFKTAMTRVFNDYARRINELKTKDKNLDGNDIREGLTAVVSVRIPEELL
                     QFEGQTKSKLGTSEARSAVDSVVADKLPFYLEEKGQLSKSLVKKAIKAQQAREAARKA
                     REDARSGKKNKRKDTLLSGKLTPAQSKNTEKNELYLVEGDSAGGSAKLGRDRKFQAIL
                     PLRGKVINTEKARLEDIFKNEEINTIIHTIGAGVGTDFKIEDSNYNRVIIMTDADTDG
                     AHIQVLLLTFFFKYMKPLVQAGRVFIALPPLYKLEKGKGKTKRVEYAWTDEELNKLQK
                     ELGKGFTLQRYKGLGEMNPEQLWETTMNPETRTLIRVQVEDEVRSSKRVTTLMGDKVQ
                     PRREWIEKHVEFGMQEDQSILDNSEVQVLENDQFDEEEI"
     gene            7062..9464
                     /gene="parC"
                     /locus_tag="ACGEXS_001630"
     CDS             7062..9464
                     /gene="parC"
                     /locus_tag="ACGEXS_001630"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFF8012639.1"
                     /translation="MSEIIQDLSLEDVLGDRFGRYSKYIIQERALPDVRDGLKPVQRR
                     ILYAMYSSGNTHDKNFRKSAKTVGDVIGQYHPHGDFSVYEAMVRLSQDWKLRHVLIEM
                     HGNNGSIDNDPPAAMRYTEAKLSLLAEELLRDINKETVSFIPNYDDTTLEPMVLPSRF
                     PNLLVNGSTGISAGYATDIPPHNLAEVIQATLKYIDNPDITVNQLMKYIKGPDFPTGG
                     IIQGIDGIKKAYESGKGRIIVRSKVEEETLRNGRKQLIITEIPYEVNKSSLVKRIDEL
                     RADKKVDGIVEVRDETDRTGLRIAIELKKDVNSESIKNYLYKNSDLQISYNFNMVAIS
                     DGRPKLMGIRQIIDSYLNHQIEVVANRTKFELDNAEKRMHIVEGLIKALSILDKVIEL
                     IRSSKNKRDAKENLIEVYEFTEEQAEAIVMLQLYRLTNTDIVALEGEHKELEALIKQL
                     RHILDNHDALLNVIKEELNEIKKKFKSERLSLIEAEIEEIKIDKEVMVPSEEVILSMT
                     RHGYIKRTSIRSFNASGVEDIGLKDGDSLLKHQEVNTQDTVLVFTNKGRYLFIPVHKL
                     ADIRWKELGQHVSQIVPIEEDEVVINVFNEKDFNTDAFYVFATQNGMIKKSTVPLFKT
                     TRFNKPLIATKVKENDDLISVMRFEKDQLITVITNKGMSLTYNTSELSDTGLRAAGVK
                     SINLKAEDFVVMTEGVSENDTILMATQRGSLKRISFKILQVAKRAQRGITLLKELKKN
                     PHRIVAAHVVTGEHSQYTLYSKSNEEHGLINDIHKSEQYTNGSFIVDTDDFGEVIDMY
                     IS"
     gene            9714..11174
                     /locus_tag="ACGEXS_001631"
     CDS             9714..11174
                     /locus_tag="ACGEXS_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFF8012640.1"
                     /translation="MKDFDSLIPGWFKEFVHVGTDLIWSQYLIGLLLTAGFFFTISSK
                     FVQLRMLPEMFRALVERPETLEDGKKGISPFQAFAISAGSRVGTGNIAGVATAIVLGG
                     PGAVFWMWVIAFIGAASAFIEATLAQVYKVHDKDGGFRGGPAYYITKGLNQKWLGIVF
                     AILITITFAFVFNTVQSNTIAESLNTQYNISPVITGIILAIVTAIIIFGGVRSIATLS
                     SLIVPIMAIIYIGMVLVILLFNLDQIVPMIGTIIKSAFGIEQVTGGAVGAAVLQGIKR
                     GLFSNEAGMGSAPNAAATAAVPHPVKQGLIQSLGVFFDTMLVCTATAIMILLYSGLKF
                     GDNAPQGVAVTQSALNEHLGSAGGIFLTIAVTLFAFSSVVGNYYYGQSNIEFLSTNRV
                     ILFIFRCLVVVLVFVGAVVKTETVWNTADLFMGLMAIVNIISIIGLSNVAFALMKDYQ
                     KQKKEGKNPVFKPENLEINLFGISAWGANKYKNSDK"
BASE COUNT         3906 a         1618 c         2164 g         3524 t
ORIGIN      
        1 aaaatgtatc aagggggatc attaaatggc tgcaaatttt aaagagcaat caaaaaaaca
       61 ttttgacttg aatggccaaa gttatactta ctatgattta aaagctgtag aagagcaagg
      121 tattactaaa gtttccaatt taccttattc aattcgtgtt ttgttagaat ctttacttcg
      181 tcaagaagat gattttgtaa ttacagacga tcatattaaa gctttaagtc agtttggaaa
      241 agatggaaat gaaggcgagg taccatttaa accttctcgt gttattttac aagatttcac
      301 aggtgtacca gccgtagttg atttagcttc tttacgtaaa gcaatggatg acgttggggg
      361 agatattact aaaattaatc cagaagtacc ggtggattta gttattgacc actcagttca
      421 agtggatagc tatgcaaatc cagaagctct tgaacgtaat atgaaattag aatttgaacg
      481 taactatgaa cgttatcagt ttttaaattg ggcaacgaaa gcatttgata attacaatgc
      541 agttcctcct gcaactggaa tagttcacca agttaactta gaatatttag caagtgttgt
      601 acatgttcgt gatgtagatg gtgaaaaaac tgcatttcca gatacattag ttggtactga
      661 ttcacataca acaatgataa atggtattgg cgtactagga tggggtgttg gtggtattga
      721 agctgaagct ggaatgcttg gacaaccttc ttatttccca attccagagg ttattggtgt
      781 acgactagta aattcattac cacaaggcgc aacagcaact gatttagcgt taagagtaac
      841 tcaagagcta cgtaaaaaag gtgttgttgg taaatttgtg gagttctttg gtccaggtgt
      901 acaacattta ccactagcag accgtgctac aattgcaaac atggcaccag agtatggagc
      961 aacttgtgga ttcttcccag ttgatgatga atctcttaaa tatatgaagt taactggtag
     1021 atcagacgaa catatcgcgc tagtaaaaga atatttgaaa caaaaccata tgttctttga
     1081 tgttgagaaa gaagatccta attatacaga tgttatcgaa ttggatttat caacagttga
     1141 agcatcgctt tcaggaccaa aacgtcctca agatttaatt ttcttaagtg atatgaaatc
     1201 atcatttgaa aattctgtaa cagctccagc aggcaaccaa ggacacggtt tagataaaag
     1261 tgaatttgat aagaaagctg aaattaactt taaagatgga tcaaaagcta caatgaaaac
     1321 aggtgatatt gcaatagcag caattacatc atgtacaaat acatctaacc cttatgtaat
     1381 gttaggtgca ggtttagttg ctaaaaaagc agttgaaaaa ggcttgaaag ttcctgaata
     1441 cgttaaaact tctctagcac caggatcaaa agttgttacc ggatatttaa gagatgctgg
     1501 cttacaacct tatttagatg atttaggctt caacttggtt ggttatggat gtacaacttg
     1561 tatcggtaat tcaggtcctt tattaccaga aattgaaaaa gcgattgctg atgaggacct
     1621 attagtgaca tctgtattat ctggtaaccg taactttgaa ggtcgtatcc atcctcttgt
     1681 taaagccaat tacctagctt caccacagtt agttgttgct tatgcattag ctggaacggt
     1741 tgatattgat ttacaaaatg aacctattgg taaaggtaat gacggtgaag atgtatattt
     1801 gaaagatatt tggccatcaa ttaaagaagt ttcagatacc gttgatagtg ttgtaacacc
     1861 tgaattattt attgaagaat ataataacgt atacaataac aacgaattat ggaatgagat
     1921 tgatgtaact gatcaacctc tatatgactt tgatcctaat tcaacataca ttcaaaatcc
     1981 atcattcttc caaggattat ctaaagaacc gggtacgatt gttccattaa atggtttacg
     2041 tgttatgggt aaattcggtg attctgtgac aactgaccac atctctccag caggtgcaat
     2101 tggtaaagat acgccagctg gtaaatattt acaagatcat caagtgccta ttcgtgaatt
     2161 taattcatat ggttcaagac gtggtaatca cgaagtaatg gttcgaggta cgtttgctaa
     2221 tatacgtatt aaaaaccaat tagcgccagg tactgaaggt ggttttacaa cttattggcc
     2281 aacaaatgaa gtaatgccta tctttgatgc tgcaatgaaa tataaagaag atggtacagg
     2341 tttagttgta ttagctggta acgattatgg tatgggttca tctcgtgact gggcagcaaa
     2401 aggtacaaac ttattaggtg ttaaaacagt tattgcacaa agttatgaac gtatccatcg
     2461 ttcaaattta gttatgatgg gtgtattacc attagagttt aaaaaaggtg aatcagctga
     2521 ttctcttggt ctagatggta cagaagaaat ttctgttaat attgatgaaa atgttcaacc
     2581 acatgactac gtcaaagtta ctgctaagaa gcaagatggt gatttggtag aatttgacgc
     2641 tatggttcgt tttgactcac ttgttgaaat ggattactat cgtcacggtg gaattttaca
     2701 aatggtttta agaaataaat tagcgcaata aaaaatagat atcacagtaa aattttaatc
     2761 ggtatttgag gcactttgat agtgctcaaa aatacttcta taaactttat gatataatag
     2821 gtcggttcaa aacctttgtg ttcttggccg gcctcttctt gtattttatg ttagaatgag
     2881 atataaagtt aaactgaaag aaggcttaaa atgatatata gtattacaga aatagaagcg
     2941 cgttatgctg aaactgataa gatgggtgta atttatcacg ggaattatgc aacttggttt
     3001 gaagttgcgc ggttggatta tatatcgaag ttaggtttta gttatgctga tatggaaaaa
     3061 caaggaatca tttcacctgt gactgacctc aatgtcaatt ataaaaagtc tattttttat
     3121 ccagaaaaag ttaaagttaa aacttgggtt gaaaaatatt cgagattacg ttcagtgtat
     3181 aaatatgaga tttttaatga aaaaggtgaa cttgcaacta caggttccac agaattgatt
     3241 tgcattaaag aagatacttt taagcctata cggttggatc gttatttccc agattggcat
     3301 gaagcttata gtaaagtgca aacgctcaat aatgaaggga aaacagtaga gataatggat
     3361 ggtattgatt ctttataagt tactgtaaat gtttggagag gcaatattaa caattaatct
     3421 ataaaaaata gccaatacgt taacattaat gtaattagcg tgaagataag ctaatatata
     3481 gcaaaaacga acttgttcat acaacatcag atgtgctaaa ccaaagtaca acttccgagg
     3541 ttcaggacac atcagaatgt gtaaacaagt tcgttttatt ttagttattt tgtggaataa
     3601 gaaatttcat cttcgtgatc aactacattt acaataatgt ggtcatcttc aaagtaccac
     3661 aaatcttttt ccgctacgac aacatttaaa tcgtcatatt gttgttcata gccaatatca
     3721 acatcttcct ttggttcaac tgtaaaagca ggactaaatc cttgcttgag ttggaattcg
     3781 ccaccatatc ttacaaaaaa cacgagcact ttattatttt caggcaactc aagttcattt
     3841 ttaaaccaag ttactgctgc atcagtaagt tctatttgca taacaatgac ctcctatatt
     3901 tattacagaa tgggagtggg ccaacttgta ttatttgtgg aatttcttat cgaaattctc
     3961 tgtgttggag cccgccccaa cctgctcatt attgtaagct gaatttccat aagcttctat
     4021 gttgggaccc ccgaccagaa ttgaaaaaac ctagttataa gcgcattttg ttcggttaac
     4081 tacagctaat ataacattgt ggagcctatg acattgattt atgtctcggt ctcaaatgtt
     4141 cttgtaaaaa aactaagtat agttgaattt taagttaaaa aaattgtttt tgcaaaaata
     4201 aaataatcca tcaactgcaa aaaaatatgc aatgatggac taatgtcaaa aaattgttac
     4261 atccatttta ttttaggttc ttcgcctcta aaaatccttg cgatattaga gcgatgtcta
     4321 attatcaata tgattgaaac taagaaacta acgactaata aaatatagtc ttgaatgata
     4381 agcgagccaa tcacacagca aattgctgca acgatacttg ctaaagaaac atatttaaaa
     4441 atcttcaata caataaagaa gataattgca agtattagta aaagtatcgg attgactccc
     4501 aagacgacac ctgcactagt tgcaacagct ttgccacctt ggaattttaa ataaacagga
     4561 taaacgtgtc caagtatagc gaataagcca acaattaaac catttgtaaa aaaagtacta
     4621 atagggccat ctgcgtgaac ttgtaaccat aaagggaaga aaacagttat gaaccctttg
     4681 aaaatatcta gaaatgttac caagaatcct gcaggacgac ctaatactct aaagctatta
     4741 gtagcgccag tattaccact accaaattgt ctaatatctt ttttgaaaaa taattttcca
     4801 attacgaatc cacttgggaa agcgccgata agataactta gtagtaacat gacgattatc
     4861 atcataaata ttacacatcc tttaatatct taggactatt ttatcatatt catttaaatt
     4921 acggctaaaa attttaaaaa cggggattaa tatatggaat taagctatga aagttaattg
     4981 atacttgcat tttacgctga tttatataag aataactatt gtatagtttt aaaaacgaac
     5041 gtacgtttgc aggaggcgaa atcattggca atgaataaac aaaataatta ttcagatgat
     5101 tcaatacagg ttttagaggg gttagaagca gttcgtaaaa gacctggtat gtatattgga
     5161 tcaactgata aacggggatt acatcatcta gtatatgaaa ttgtcgataa ctccgtcgat
     5221 gaagtattga atggttacgg taacgaaata gatgtaacaa ttaataaaga tggtagtatt
     5281 tctatagaag ataatggacg tggtatgcca acaggtatac ataaatcagg taaaccgaca
     5341 gtcgaagtta tctttactgt tttacatgca ggaggtaaat ttggacaagg cggctataaa
     5401 acttcaggtg gtcttcacgg tgttggtgct tcagttgtaa atgcattgag tgaatggctt
     5461 gaagttgaaa tccatcgaga tggtaatata tatcatcaaa gttttaaaaa cggtggttcg
     5521 ccatcttctg gtttagtgaa aaaaggtaaa actaagaaaa caggtaccaa agtaacattt
     5581 aaacctgatg acacaatttt taaagcatct acatcattta attttgatgt tttaagtgaa
     5641 cgactacaag agtctgcgtt cttattgaaa aatttaaaaa taacgcttaa tgatttacgc
     5701 agtggtaaag agcgtcaaga gcattaccat tatgaagaag gaatcaaaga gtttgttagt
     5761 tatgtcaatg aaggaaaaga agttttgcat gacgtggcta cattttcagg tgaagcaaat
     5821 ggtatagagg tagacgtagc tttccaatat aatgatcaat attcagaaag tattttaagt
     5881 tttgtaaata atgtacgtac taaagatggt ggtacacatg aagttggttt taaaacagca
     5941 atgacacgtg tatttaatga ttatgcacgt cgtattaatg aacttaaaac aaaagataaa
     6001 aacttagatg gtaatgatat tcgtgaaggt ttaacagctg ttgtgtctgt tcgtattcca
     6061 gaagaattat tgcaatttga aggacaaacg aaatctaaat tgggtacttc tgaagctaga
     6121 agtgctgttg attcagttgt tgcagacaaa ttgccattct atttagaaga aaaaggacaa
     6181 ttgtctaaat cacttgtgaa aaaagcgatt aaagcacaac aagcaaggga agctgcacgt
     6241 aaagctcgtg aagatgctcg ttcaggtaag aaaaacaagc gtaaagacac tttgctatct
     6301 ggtaaattaa cacctgcaca aagtaaaaac actgaaaaaa atgaattgta tttagtcgaa
     6361 ggtgattctg cgggaggttc agcaaaactt ggacgagacc gcaaattcca agcgatatta
     6421 ccattacgtg gtaaggtaat taatacagag aaagcacgtc tagaagatat ttttaaaaat
     6481 gaagaaatta atacaattat ccacacaatc ggggcaggcg ttggtactga ctttaaaatt
     6541 gaagatagta attataatcg tgtaattatt atgactgatg ctgatactga tggtgcgcat
     6601 attcaagtgc tattgttaac attcttcttc aaatatatga aaccgcttgt tcaagcaggt
     6661 cgtgtattta ttgctttacc tccactttat aaattggaaa aaggtaaagg caaaacaaag
     6721 cgagttgaat acgcttggac agacgaagag cttaataaat tgcaaaaaga acttggtaaa
     6781 ggcttcacgt tacaacgtta caaaggtttg ggtgaaatga accctgaaca attatgggaa
     6841 acgacgatga acccagaaac acgaacttta attcgtgtac aagttgaaga tgaagtgcgt
     6901 tcatctaaac gtgtaacaac attaatgggt gacaaagtac aacctagacg tgaatggatt
     6961 gaaaagcatg ttgagtttgg tatgcaagag gaccaaagta ttttagataa ttctgaagta
     7021 caagtgcttg aaaatgatca atttgatgag gaggaaatct agtgagtgaa ataattcaag
     7081 atttatcact tgaagatgtt ttaggtgatc gctttggaag atatagtaaa tatattattc
     7141 aagagcgtgc attgccagat gttcgtgatg gtttaaaacc agtacaacgt cgtattttat
     7201 atgcaatgta ttcaagtggt aatacacacg ataaaaattt ccgtaaaagt gcgaaaacag
     7261 tcggtgatgt tattggtcaa tatcatccac atggagactt ctcagtgtac gaagcaatgg
     7321 tccgtttaag tcaagactgg aagttacgac atgtcttaat agaaatgcat ggtaataatg
     7381 gtagtatcga taatgatccg ccagcggcaa tgcgttacac tgaagctaag ttaagcttac
     7441 tagctgaaga gttattacgt gatattaata aagagacagt ttctttcatt ccaaactatg
     7501 atgatacgac actcgaacca atggtattgc catcaagatt tcctaactta ctagtgaatg
     7561 gttctacagg tatatctgca ggttacgcga cagatatacc accacataat ttagctgaag
     7621 tgattcaagc aacacttaaa tatattgata atccggatat tacagtcaat caattaatga
     7681 aatatattaa aggtcctgat tttccaactg gtggtattat tcaaggtatt gatggtatta
     7741 aaaaagctta tgaatcaggt aaaggtagaa ttatagttcg ttctaaagtt gaagaagaaa
     7801 ctttacgcaa tggacgtaaa cagttaatta ttactgaaat tccatatgaa gtgaacaaaa
     7861 gtagcttagt aaaacgtatc gatgaattac gtgctgacaa aaaagtcgat ggtatcgttg
     7921 aagtacgtga tgaaactgat agaactggtt tacgaatagc aattgaattg aaaaaagatg
     7981 tgaacagtga atcaatcaaa aattatcttt ataaaaactc tgatttacag atttcatata
     8041 atttcaacat ggtcgctatt agtgatggtc gtccaaaatt gatgggtatt cgtcaaatta
     8101 tagatagtta tttgaatcac caaattgagg ttgttgcaaa tagaacgaag tttgaattag
     8161 ataatgcaga aaaacgtatg catatcgttg aaggtttgat taaagcgttg tcaattttag
     8221 ataaagtaat cgaattgatt cgtagctcta aaaacaagcg tgacgctaaa gaaaacctta
     8281 tcgaagtata cgagttcaca gaagaacagg ctgaagcaat tgtaatgtta cagttatatc
     8341 gtttaacaaa tactgacata gttgcgcttg aaggtgaaca taaagaactt gaagcattaa
     8401 tcaaacaatt acgtcatatt cttgataacc atgatgcatt attgaatgtc ataaaagaag
     8461 aattgaatga aattaaaaag aaattcaaat ctgaacgact gtctttaatt gaagcagaaa
     8521 ttgaagaaat taaaattgac aaagaagtta tggtgcctag tgaagaagtt attttaagta
     8581 tgacacgtca tggatatatt aaacgtactt ctattcgtag ctttaatgct agcggtgttg
     8641 aagatattgg tttaaaagat ggtgacagtt tacttaaaca tcaagaagta aatacgcaag
     8701 ataccgtact agtatttaca aataaaggtc gttatctatt tataccggtt cataaattag
     8761 cagatattcg ttggaaagaa ttgggacaac atgtatcaca aatagttcct atcgaagaag
     8821 atgaagtggt tattaatgtc tttaatgaaa aggactttaa tacagatgca ttttatgttt
     8881 ttgcgactca aaatggcatg attaagaaaa gtacagtgcc tctatttaaa acaacgcgtt
     8941 ttaataaacc tttaattgct actaaagtta aagaaaatga tgatttgatt agtgttatgc
     9001 gctttgaaaa agatcaatta attaccgtca ttactaataa aggtatgtca ttaacgtata
     9061 atacaagtga actatcagat accggattaa gggcagctgg tgttaaatca ataaatctta
     9121 aagctgaaga tttcgttgtt atgacagaag gtgtttctga aaatgatact atattgatgg
     9181 ccacacaacg cggctcgtta aaacgtatta gttttaaaat cttacaagtt gctaaaagag
     9241 cacaacgtgg aataacttta ttaaaagaat taaagaaaaa tccacatcgt attgtagctg
     9301 cacatgtagt gacaggtgaa catagtcaat atacattata ttcaaaatca aatgaagaac
     9361 atggtttaat taatgatatt cataaatctg aacaatatac aaatggctca ttcattgtag
     9421 atacagatga ttttggtgaa gtaatagaca tgtatattag ctaaaaacta tatgcaatca
     9481 cgaaattaaa tgataaaata cagtaatgtt aaattttgac taaattcaag ggatttatat
     9541 taaatgctga ccaagtactt atcgttaaat tagcgatata ctttgaccaa actaatataa
     9601 atctcttgta ttgtataaga aatgatgatg tatctctata tttactgaag gttcaattcg
     9661 gtctaatttt atagaaggta catgattcat gtagaggatg cgagggattt attttgaaag
     9721 atttcgatag tttaatacct ggatggttta aagaatttgt ccatgttggt accgatttaa
     9781 tatggtctca atatttaatt ggtctattat tgacagctgg attcttcttt acgattagtt
     9841 ctaaattcgt tcaattacga atgttacctg aaatgtttag agctttagta gaacgtccag
     9901 aaactttaga agatggtaag aagggtattt cgccattcca agcatttgcg attagtgctg
     9961 gttcgagagt tggtactggt aatattgctg gtgttgcgac tgcgattgtt ttaggcggtc
    10021 caggtgcagt attttggatg tgggttattg catttatagg tgcagcgagt gcatttatag
    10081 aagcgacttt ggctcaggtt tataaagtac atgataaaga tggtggattc cgtggtggtc
    10141 cagcttacta tattactaaa ggtttaaatc aaaaatggct aggtatcgta tttgcgattt
    10201 taattacaat tacatttgca tttgtattta acacagtgca atctaataca attgcggagt
    10261 cgttaaatac gcaatataat attagtccag taatcacagg tattatttta gcaatcgtaa
    10321 cagctattat tatatttggt ggtgtacgta gtattgctac gttatcttcg ttaattgtac
    10381 cgattatggc tatcatttac attggtatgg ttttagtaat attgctattt aatttagatc
    10441 aaattgttcc tatgataggt acgattatta aaagtgcatt tggtatcgaa caagtaactg
    10501 gtggcgctgt aggtgctgcg gttcttcaag gtatcaaacg tggtttattc tctaacgaag
    10561 ctggtatggg ttctgcgccg aatgcagcgg caactgctgc cgtaccacac cctgttaagc
    10621 aaggtttaat ccaatcatta ggtgtgttct ttgatacaat gttggtttgt acagcaactg
    10681 caatcatgat tttactatat tcaggactga aatttggtga taacgcacct caaggtgttg
    10741 cagttactca atcagcactt aatgagcatt taggttctgc tggaggtatt ttcttaacaa
    10801 tagcagttac actgtttgca ttttcatctg ttgtaggtaa ttactattac ggtcaatcta
    10861 atattgaatt tttatcaaca aaccgtgtaa tattatttat ctttagatgt cttgttgtag
    10921 tacttgtctt tgtcggtgca gttgtaaaaa cagaaacagt atggaatacg gcagacttat
    10981 ttatgggctt aatggcaatt gtaaacatta tttccattat aggactgtcc aatgtagctt
    11041 ttgcattgat gaaagattat caaaagcaga aaaaagaagg caagaaccct gtctttaaac
    11101 ctgaaaactt agaaattaac ttatttggaa ttagtgcttg gggcgctaac aaatataaga
    11161 actctgataa ataaatattt ttaatgtaac gattcattat ataaaaatca aa
//
LOCUS       DAXWFK010000083        11112 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.083, whole genome shotgun sequence.
ACCESSION   DAXWFK010000083 DAXWFK010000000
VERSION     DAXWFK010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11112
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.083"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..125
                     /locus_tag="ACGEXS_001632"
     CDS             <1..125
                     /locus_tag="ACGEXS_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012641.1"
                     /translation="KTMSIENMRTYQQRVNNLWIKGVDNVIYCGYQVGKQKIND"
     gene            complement(270..2198)
                     /locus_tag="ACGEXS_001633"
     CDS             complement(270..2198)
                     /locus_tag="ACGEXS_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFF8012642.1"
                     /translation="MILLQLNHISKSFDGEDIFTDVDFEVKTGERIGIVGRNGAGKST
                     LMKIIAGVENYDSGNVSKIKNLKLGYLTQQMTLNSNATVFEEMSKPFEHIKRMESLIK
                     EETDWLSKHANDYDSDTYKTHMSRYESLSNQFEQLEGYQYESKIKTVLYGLNFSEEDF
                     NKPINDFSGGQKTRLSLAQMLLNEPDLLLLDEPTNHLDLETTKWLEDYLRYFKGAIVI
                     ISHDRYFLDKIVTQIYDVALGDVKRYVGNYEEFIQQRDLYYQKRMQEYESQQAEIKRL
                     ETFVEKNITRASTSGMAKSRRKILEKMERIDKPMLDAKSANIQFGFDRNTGNDVMHVK
                     NLEIGYQTAITKPMSIEVSKGDHIAIIGPNGIGKSTLIKTIANQQKALNGDITFGANL
                     QIGYYDQKQAEFKSSKTILDYVWDQYPLMNEKDIRAVLGRFLFVQDDVKKIINDLSGG
                     EKARLQLALLMLQRDNVLILDEPTNHLDIDSKEMLEQALQHFEGTILFVSHDRYFINQ
                     LANKVFDLTIDGGKMYLGDYQYYIEKVEEAEALKAHQEEQSVNVQAHKKSMEQSSYHN
                     QKEQRREQRKLERQISECENEIETLETTILQIDEQLTQPEVYNNPQKANELAIQKQDS
                     EQKLEHAMSKWEELQQKL"
     gene            2451..3086
                     /locus_tag="ACGEXS_001634"
     CDS             2451..3086
                     /locus_tag="ACGEXS_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFF8012643.1"
                     /translation="MSDQVKIPRATLKRLPLYYRFVSSLKSKGIDRVNSKAISDALQI
                     DSATIRRDFSYFGELGKKGYGYNIDSLLDFFKSELSESDMIKIAIVGVGNLGKALLTY
                     NFSIHDDMTITEAFDVKEDVIGQKIGNVIVKDNDELITTLKKEEIDVVILTTPERVAQ
                     KVADELVQAGVKGILNFTPGRINTPSDVQVHQIDLGIELQSLLFFMKNYSE"
     gene            3390..4469
                     /locus_tag="ACGEXS_001635"
     CDS             3390..4469
                     /locus_tag="ACGEXS_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HFF8012644.1"
                     /translation="MWMIFSGLIVGGLLGFVMQRTRFCLTGGFRDMYVQKNNKMFYAL
                     LIAITIQSIGLLILTATDILQIPAHSFPILGTIIGSFIFGIGIVLAGGCATGTWYRAG
                     EGLIGSWIALVLYAVTAAITKTGILKPVMDKINQPTNVNSDMSQTTGIPFWGLVVILT
                     IITIFLVVRTLNNKKVRVAVPKLKQRYTGIRYYLFEKRYHPFIAAIVIGLIALLAWPM
                     SASTGRNDGLGITTPSANLVHFLITGETKFIDWGVFLVLGIFIGSYIAARGSREFKWR
                     LPDKITIRNSAIGGICMGFGASVAGGCSIGNGLVETATMTWQGWIALASMIVGVWTMS
                     HFIFVRPMKKVHQQSAKVKQQTQIV"
     gene            4529..4753
                     /locus_tag="ACGEXS_001636"
     CDS             4529..4753
                     /locus_tag="ACGEXS_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HFF8012645.1"
                     /translation="MIHELGTVGMVCPFPLIEAQKKMATLQSGDELKIDFDCTQATEA
                     IPNWAAENGYPVTNYEQIDNASWTITIQKV"
     gene            4961..6211
                     /locus_tag="ACGEXS_001637"
     CDS             4961..6211
                     /locus_tag="ACGEXS_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFF8012646.1"
                     /translation="MNLNDTIFMFLCTLLVWLMTPGLSLFYGGLVQSKNALNTVMQSM
                     AAIVLVTFVWITVGFTISFGNGNLWFGNWEYTFLNHVGFATQEDISPHIPFALFMLFQ
                     MMFCTIAISILSGSIAEKMKFIPYLLFVVIWTALVYSPVAHWVWGGGWINKLGVLDFA
                     GGTVVHITSGVSGLVLAIMIGKGNKHSESTPHNLIITLIGGIFVWIGWYGFNVGSAFT
                     FDNIAMLAFTNTVISASAGAIGWLILEYIFKKTTSLLGLLLGALAGLVVITPAAGYVT
                     YLSATIMALIGGICCYIVINYIKVKLKYHDALDAFGIHGVGGIIGAVLTAVFQSKKAN
                     PDIENGFIYTGDIHIILVQILCVTAVVIFSIVMTFIIAKVIKLITPLSVTEQETNIGL
                     DKIVHGEHAYFEGELNRFNKHIRY"
     gene            6394..7344
                     /locus_tag="ACGEXS_001638"
     CDS             6394..7344
                     /locus_tag="ACGEXS_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8012647.1"
                     /translation="MKNISDIAKLAGVSKSTVSRFLNNGSVSKKTSEKLTRIIAEHDY
                     QPNQFAQSLRARQTHLIGAIIPRMNSYAVDETIKGLAKQCQKYESQLILNYTGLNIEA
                     EIQALETLARSKVDGIVLMATDITERHIEVINKMNVPIVIVGQQHEQLHSIVHDDYKA
                     GQIIGEWIGQQGYQQVEVFSVSEKDIAVGIHRKRGLLDQLAKYQIKPNIHETNFTYVE
                     AQKDVANVLENVEQVDAVVGATDTIALAAYKYYSDKKDVMKPHQIYGFGGDPMTQLVS
                     PSIKTIHYNYFEAGQCAMEEIQQMLKKQDMPYSVTVDVNI"
     gene            7493..8977
                     /locus_tag="ACGEXS_001639"
     CDS             7493..8977
                     /locus_tag="ACGEXS_001639"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFF8012648.1"
                     /translation="MTEWTREERYQRIEDVDTEYFKTLKQQVDQSKFRQQFHIQPETG
                     LLNDPNGLIFYKGKYYVSHQWFPLGAVHGLKYWYNYTSDDLINFKAEGPILNPDTKYD
                     SHGVYSGSAFEYNRHLYYMYTGNHRDNHWQRHASQMIARLKEDGSVEKFPKPVISQQP
                     EGYTSHFRDPKVFKYDEKYYAIIGAQNNDQQGRLLLYNTEDIINWHYLGEINTELDDF
                     GYMWECPDYFNVDNQDVILICPQGIEPKGDQFKNIYQSGYILGKFDIEKLTYEHENFV
                     ELDNGFDFYAPQTFLDEKGRRVLIGWMGLPEIEYPTDNEGWAHCLTIPRVLNVENGQL
                     KQRPYPALEKLRHNKETALGYANKFTRKLHPYEGKQYELIIDILDNDATEVYFELRTS
                     KTSSTLIAYNKRENKITLDRSDSGLLPTNVEGTTRSTILDTPLKQLQIFVDTSSIEIF
                     CNDGERVLTSRIFPTEDALGIKTSTESGQVYLQFTKYDLKDEHK"
     gene            8974..9933
                     /locus_tag="ACGEXS_001640"
     CDS             8974..9933
                     /locus_tag="ACGEXS_001640"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HFF8012649.1"
                     /translation="MRRLFSIGEALIDFIPNVTNANLKDVQTFTKQIGGAPCNVACTV
                     QKLGQQAYMITQLGNDAFGDSIIETISSIGVDVSKVYRTNEANTALAFVSLTEAGERD
                     FSFYRKPSADMLFEPSFVNDIDVNENDVVHFCSVDLVDSPMRDAHYQLITKTLNANGT
                     VVFDPNVRLPLWDNAEDLRQTIHTFLPLAHIVKVSDEELEFITGIHDENEAIQSLFTG
                     NVTVVIYTKGADGAAVYLKNGINHYHSGYKVKPVDTTGAGDAFIGAVISRILATDVLN
                     LTQLFENEGEEILAFSNRVAAIVTTKYGAINSLPTLQEVEQAF"
     gene            complement(10121..10228)
                     /locus_tag="ACGEXS_001641"
     CDS             complement(10121..10228)
                     /locus_tag="ACGEXS_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012650.1"
                     /translation="MHCIAGFLILLTKLNMTRELKNDVTSLCIPIPITT"
     gene            complement(10300..11016)
                     /gene="agrA"
                     /locus_tag="ACGEXS_001642"
     CDS             complement(10300..11016)
                     /gene="agrA"
                     /locus_tag="ACGEXS_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing response regulator AgrA"
                     /protein_id="HFF8012651.1"
                     /translation="MKIFICEDDPKQRENMVTIIKNYIMIEEKPMEIALATDNPYEVL
                     EQAKNMNDIGCYFLDIQLSTDINGIKLGSEIRKHDPVGNIIFVTSHSELTYLTFVYKV
                     AAMDFIFKDDPAELRTRIIDCLETAHTRLQLLSKDNSVETIELKRGSNSVYVQYDDIM
                     FFESSTKSHRLIAHLDNRQIEFYGNLKELSQLDDRFFRCHNSFVVNRHNIESIDSKER
                     IVYFKNKEHCYASVRNVKKI"
BASE COUNT         3745 a         1565 c         2007 g         3795 t
ORIGIN      
        1 taaaaacaat gtcaatagag aacatgcgta cttatcaaca aagagtgaat aacttgtgga
       61 taaaaggtgt tgataacgtc atttattgtg gatatcaggt gggaaaacag aaaataaatg
      121 attgattagt gttaatttac ttgtgggtaa ctaggataag tctgtggata gctataatat
      181 cagcgttttt attgtgaata aaaaaaatta aaatttgtaa aaaaataact ctaactatag
      241 cgattttaca gttagagtta tgtatatttt tatagttttt gttgtaattc ttcccattta
      301 gacatagcgt gttctaattt ttgttcgcta tcttgttttt ggatagctaa ttcgtttgct
      361 ttttgtggat tgttatacac ttctggttgg gttaattgct cgtctatttg taagatagta
      421 gtctctaaag tttctatttc attttcacac tcggatattt gtcgttcaag tttgcgttgt
      481 tcacgtcttt gttctttttg gttatgatac gacgattgtt ccatagactt tttatgtgct
      541 tgtacgttaa ctgactgttc ttcttggtgc gctttcaacg cttccgcttc ctcaactttt
      601 tcaatgtaat attgataatc tcctaaatac atctttccgc catcaattgt taaatcaaat
      661 accttgtttg ctaattggtt aataaagtaa cgatcatggg aaacaaatag tattgttcct
      721 tcaaaatgtt ggagtgcttg ctctaacatt tcttttgaat ctatatcgag gtggttggta
      781 ggttcatcta aaataagtac attgtcacgt tgcaacataa gtagtgctag ttgtaaacgt
      841 gctttttcac caccagataa atcattaatt atctttttaa catcgtcttg tacaaataag
      901 aaacgtccaa gaactgctcg aatatctttt tcattcatta acggatattg atcccacaca
      961 taatctaaaa tcgttttact agatttaaat tctgcttgct tttgatcata ataaccaatt
     1021 tgtaaatttg cgccgaaagt aatatcgcca ttaagcgctt tttgttgatt agcaatagtt
     1081 ttaattaagg tcgattttcc aataccattt ggcccaatga ttgctatatg atcgccttta
     1141 gagacctcta tactcatagg tttggtaatt gcagtttgat aaccgatttc taaatttttt
     1201 acatgcatga cgtcattacc tgtattccgg tcaaagccaa attgaatatt tgcacttttg
     1261 gcatctaaca ttggtttatc aatgcgttcc attttttcta aaatcttacg tctacttttt
     1321 gccattccac ttgttgaagc acgggtaata tttttctcaa caaaagtttc taatcgtttt
     1381 atttctgctt gttgactttc atattcttgc attcgttttt gataatataa atcccgttgc
     1441 tgtataaatt cctcgtaatt accaacatag cgtttgacat cacctaaagc cacatcataa
     1501 atttgagtaa ctattttatc taaaaagtaa cgatcatggc tgatgatgac gattgcacct
     1561 ttaaaataac gtagataatc ttcaagccac tttgtcgttt ctaaatctaa gtggttagta
     1621 ggttcatcta aaagtaataa atcaggttcg tttaatagca tttgagctaa agataaacgt
     1681 gttttttggc caccgctaaa atcattgata ggtttattga aatcttcttc actaaaattt
     1741 aacccgtaaa gtactgtttt aattttactt tcatattgat atccttctaa ttgttcaaat
     1801 tgattcgata aagattcata acgggacata tgtgttttat atgtatcact atcgtaatca
     1861 tttgcatgtt ttgataacca atctgtttct tctttgatta agctttccat tcgtttaata
     1921 tgttcaaatg gttttgacat ttcttcaaaa actgttgcat tagaatttaa agtcatttgt
     1981 tgagttaaat aacctagttt taagttttta atttttgaca catttccaga atcatagttt
     2041 tctacgccag ctataatttt catcaatgtt gatttaccgg cgccatttct tcctacgata
     2101 ccaattcgtt caccagtttt tacttcaaaa tcaacatcag taaatatatc ttcaccatcg
     2161 aacgattttg atatatgatt gagttgtaaa agtatcattc gatcttcacc tttcgtcttt
     2221 ctcaaatttt attaacttat gaaaaaagtc gtaattatta cattaataac cattttacag
     2281 tataaaacgc cgtcttgaaa ctaagatatt tatttttaaa attcaataat gtataatatt
     2341 taggtattca ttgtaatcaa ttatttgttt tttaataatc taacaattac gctctaactc
     2401 gtttgaatac atagatataa ttcattcaaa cacgaaggag gaaatagcga atgagtgacc
     2461 aagttaaaat tcctcgagca actttaaaac gtttgccgtt atattataga tttgtcagtt
     2521 cattaaaatc taaaggtata gatcgtgtaa attcaaaagc gattagcgat gcgttacaaa
     2581 ttgactcggc aacaattcgt cgtgactttt catattttgg cgaattaggt aaaaaagggt
     2641 acggatataa tatagatagt ttattggatt tctttaaatc tgaactaagc gagagtgaca
     2701 tgatcaaaat cgcaattgtc ggagttggga acctagggaa agctttgctc acatataact
     2761 tttcaataca tgacgatatg acgattacag aagcgtttga cgtaaaagaa gatgttattg
     2821 gccagaaaat agggaacgtt attgttaaag ataacgatga attaataaca acattgaaga
     2881 aggaagaaat agatgttgtg attctaacta caccagaaag agttgcacag aaagttgcag
     2941 atgaactcgt ccaagctggt gtgaaaggta ttttaaactt cactcctggt agaattaata
     3001 cgccttcaga tgtgcaagta catcaaattg acttaggtat agaattacag tcattattat
     3061 tctttatgaa aaattacagt gaataaatgt gatgttagct ttgaatgatt aaccttgtta
     3121 tatctttaat taattataga acagtctggg actaagtatg tctcaggctt tttctgttat
     3181 gtttgaaaat gaaaggaaaa actgttgtgt tgataaatat agtataggtt aataaattaa
     3241 cacgtagaga tataatgatg aatcgtaatt aatcgccttc cttatatgaa tataatataa
     3301 atatcaaaaa tattgtgtat aaggttgaaa acaagaaaat catcacctat aatacatact
     3361 aaacttatcg gaaatgagag gttaggatta tgtggatgat ttttagtggc ttaattgtcg
     3421 ggggactctt aggttttgtc atgcaaagaa caagattttg tttaacaggt ggctttcgag
     3481 atatgtatgt gcaaaagaat aataagatgt tctatgcatt attaatcgct attactattc
     3541 aaagtatagg attattgatt ttgacggcaa cagatatttt acaaattcct gcacatagtt
     3601 ttccaatatt gggaacaatt ataggttctt ttatttttgg aattggaata gtattggctg
     3661 gaggatgtgc aacaggtact tggtatcgcg ctggtgaagg gctaattggt agttggattg
     3721 cattagtatt atatgctgtt actgcagcaa tcactaaaac agggatttta aagccagtaa
     3781 tggataaaat taatcaacca acgaatgtaa atagtgatat gtctcaaaca actggcattc
     3841 cgttttgggg attagtcgtt atattaacta taatcaccat ttttctagtt gtaagaacac
     3901 tcaataataa gaaagttcga gttgctgttc caaaactaaa gcaacgttat acaggtatta
     3961 gatattacct tttcgaaaaa cgataccatc catttattgc agcaattgta attggactta
     4021 tcgcactctt agcttggcca atgagtgcat caactggaag aaatgacggt ttaggtataa
     4081 caacgccttc agcaaattta gtacactttt tgattacagg tgaaactaaa tttattgatt
     4141 ggggtgtctt tttagttcta ggaattttca ttggttcata tattgcagct agaggatcaa
     4201 gagaatttaa atggcgattg ccagacaaga ttacaatacg aaacagtgcc attggtggca
     4261 tatgtatggg atttggtgcg tcagttgctg gtggttgttc tatcggtaac ggtttggttg
     4321 aaacggcaac gatgacttgg caaggatgga ttgcgctagc atcgatgata gttggtgtat
     4381 ggacaatgag tcattttatc tttgttcgtc caatgaaaaa agtacaccaa caatctgcaa
     4441 aggttaaaca gcaaacgcaa atagtataga agattattat gcaaatgatg ttgatcaaat
     4501 aaaagtgatt ggaaaaggag aaataattat gatacacgaa ttaggtacag taggaatggt
     4561 atgtccattt ccgttaattg aagcgcaaaa gaaaatggca acattgcaat ctggagatga
     4621 attaaaaatt gattttgatt gcacgcaagc gacggaagcc attccaaatt gggctgcaga
     4681 aaatggttat cctgtaacaa actatgaaca aattgataat gcttcatgga caattacaat
     4741 tcaaaaagtt taacgttatc attttaacaa taaaatagat attagattct atggctactt
     4801 ccgctaattt aaaagtgagt aagtagtctt ttttgtttta gttcatgaaa tcatttttat
     4861 atagtgtggc acattttatt ccaaaagatg taataaaact taacgcactt ttgcttttta
     4921 taaattgtca gattattatg aaaaaaaggg agtggtaagt atgaatctta acgatacgat
     4981 atttatgttt ttgtgtacat tattagtttg gttaatgaca ccaggattaa gtttatttta
     5041 tggtgggtta gttcaatcta aaaatgcgct taatactgtc atgcaaagta tggcagcaat
     5101 tgtgcttgtt acatttgtat ggataacagt tggttttaca attagttttg ggaatgggaa
     5161 tttatggttc ggaaattggg aatatacttt tcttaatcat gtaggttttg cgactcaaga
     5221 agatattagc ccacatattc ctttcgcttt gtttatgtta tttcaaatga tgttttgtac
     5281 gattgcaatt tctattttat ctggttcaat cgctgagaaa atgaagttta ttccttattt
     5341 attattcgta gtaatatgga ctgctcttgt atacagtcca gtagcacatt gggtttgggg
     5401 cggcggttgg attaacaaac tcggtgtatt agatttcgct ggaggtacgg ttgttcatat
     5461 tacatcaggt gtttctggtt tagtattagc tattatgatt ggaaaaggaa acaaacattc
     5521 tgaatcaaca ccacataatc ttatcattac gttgattggc ggtatattcg tgtggattgg
     5581 ttggtatgga tttaatgtag gtagtgcttt tacatttgat aatattgcga tgcttgcatt
     5641 tacaaatact gtcatttcag ccagtgcagg tgctataggt tggttaattt tagaatatat
     5701 ttttaaaaag acgacaagtt tacttggact tttactcggt gcattagcag gattagttgt
     5761 cattactcct gcagcaggat atgtaacata tcttagtgca acaataatgg ctttaatagg
     5821 aggtatctgt tgttatattg tcattaatta catcaaggta aaactaaaat atcatgatgc
     5881 attagatgca tttggtattc atggtgttgg tggtattatt ggtgctgttt taacagcagt
     5941 tttccaaagt aaaaaagcca atcctgacat tgagaatggc tttatttata ctggtgacat
     6001 acatattata cttgtacaaa tattatgtgt aacagcagtt gtaattttta gtatagtcat
     6061 gacgtttatt attgcgaaag taattaaatt aattacacca ttatctgtta cggaacaaga
     6121 aacgaatata ggattagaca agattgttca cggtgaacat gcttactttg aaggtgagct
     6181 aaatagattc aataaacata ttcgatatta gaatatattt acatagaata ttcattgtcc
     6241 tgacatttaa ctaaaggttg atgttgggac attttgttat acaaaagttt tattttgaaa
     6301 tctttttatg aaagaagcag aaatattatt taaagcggtt acacatatgc taaaataagg
     6361 ctaagtgtca caaataatga taggtgaata agtatgaaaa atatatctga tattgccaaa
     6421 ttggcaggcg tttcaaaaag tacagtatct agatttttaa ataatggatc tgtcagtaaa
     6481 aaaacaagtg aaaaattaac aagaattata gcagaacatg actatcaacc gaatcaattt
     6541 gctcaaagtt taagagcgag acaaacacac cttattggtg ccattatacc tagaatgaat
     6601 tcatatgcag tagatgaaac aatcaaagga ttggcaaaac aatgccaaaa atatgaatca
     6661 caattaattt taaattacac aggtttaaat atcgaagcag aaatacaagc gcttgaaaca
     6721 ttagcacgta gtaaagtaga tggtattgtt ttaatggcta cagacataac agagagacat
     6781 attgaagtca ttaataaaat gaatgtacca atcgttattg ttggtcaaca acatgaacaa
     6841 cttcatagta ttgtgcatga tgattataaa gcaggtcaaa ttataggcga atggattggt
     6901 caacagggat atcaacaagt tgaagtgttt agtgtaagtg aaaaagatat tgcagttggt
     6961 atacatagaa aacgtggttt acttgaccag ttagctaaat accaaattaa acctaatata
     7021 catgaaacaa attttactta tgtggaagca caaaaagatg ttgcaaatgt tttggaaaat
     7081 gtggagcaag tagatgcggt tgttggagca actgatacga ttgcattagc tgcctataaa
     7141 tattattctg ataaaaaaga tgttatgaaa ccacatcaaa tatatggttt tggtggtgac
     7201 ccaatgacac aattagtgtc tccatcgata aaaacaattc attataatta ttttgaagct
     7261 ggccaatgcg cgatggaaga gatacaacag atgcttaaaa agcaagatat gccatatagc
     7321 gtcacagtag atgttaatat ttagacgctg tattttttaa aataaatgtg gaaccgatac
     7381 catataacta taaatggata ggttaaaagt taaagaacgt aggtaaaatt tgctataata
     7441 gaatataaat tgttaacagc ataaattata aaaggaggac tgggtaaata ttatgaccga
     7501 atggactaga gaagaacgtt atcaacgaat cgaggacgtt gatactgagt attttaaaac
     7561 attaaaacaa caagttgatc aatcaaaatt tcgtcaacaa tttcatatac aaccagaaac
     7621 aggcttatta aatgacccca acggacttat tttttataaa gggaagtatt atgtttcaca
     7681 tcaatggttc ccattaggcg cagtacatgg cttaaagtat tggtataact acacgagtga
     7741 tgacttaata aactttaaag ctgaagggcc aattttaaat ccagatacta aatatgacag
     7801 ccatggtgta tatagcggta gcgcttttga atataacagg catttatatt atatgtacac
     7861 aggaaatcat cgagataatc attggcaacg acatgcgagt cagatgatcg cacgattgaa
     7921 agaagacggt tcagttgaaa agtttccaaa gccagtaatt agccaacaac cagaaggata
     7981 tacgagtcat tttagagatc ctaaagtttt taaatatgat gagaaatatt atgcaatcat
     8041 tggtgcacaa aataatgatc agcaaggtcg attattactt tataatactg aagatataat
     8101 taattggcat tatttaggtg aaataaatac agagttggat gattttggat atatgtggga
     8161 atgcccagat tactttaatg tagataatca agatgtcata cttatttgtc cacaaggtat
     8221 tgaaccaaaa ggcgatcagt tcaaaaatat ttatcaaagt ggttatatac ttggaaagtt
     8281 tgatattgaa aagttaacat atgaacatga aaattttgtc gagcttgata atggttttga
     8341 tttctatgca cctcaaacat ttttagatga aaaaggccga cgagtactaa ttggatggat
     8401 ggggttaccg gaaatcgaat atcctactga taatgaagga tgggcccatt gcttaacaat
     8461 tcctcgtgta ttaaatgtag aaaatggtca acttaagcaa cgtccatatc cagcgttgga
     8521 aaaattacgt cacaataaag agacagcatt aggctacgca aataaattta ctcgaaaatt
     8581 acatccgtat gaaggtaaac agtatgaatt aatcatagat attttggata atgatgctac
     8641 cgaagtgtac tttgaattac gtacatctaa gacttcttca acattaattg cttataacaa
     8701 gcgtgaaaat aaaataacat tagatcgcag cgacagtggt ttattgccga caaatgttga
     8761 aggtacgacg cgtagtacga tattagacac gccattaaag caattacaaa tttttgttga
     8821 tacatctagt atagaaattt tctgtaatga tggagaacgt gttttgacat ctcgaatttt
     8881 cccgacagaa gacgcactag gtattaaaac atcaacagaa tcagggcaag tatatttaca
     8941 atttactaaa tatgatttaa aggatgaaca taaatgagac gtctattttc aataggagaa
     9001 gcgttaattg attttattcc aaatgttaca aatgcgaatt taaaagatgt tcaaacattt
     9061 acgaagcaaa ttggtggcgc gccatgcaat gtagcttgta cagttcaaaa gttaggacaa
     9121 caagcgtata tgattacaca attaggtaat gatgcatttg gagatagcat tattgaaacg
     9181 atatcaagta ttggtgtaga tgtatcaaaa gtgtatcgca cgaatgaagc aaacactgcg
     9241 ttagcttttg tgagtttgac tgaagcgggc gagcgagatt tttcttttta tcgtaagccc
     9301 tctgctgata tgttatttga accaagtttt gtgaatgata ttgatgtgaa tgaaaatgat
     9361 gtagtacatt tttgttctgt tgatttggtc gatagtccga tgagagatgc acattatcaa
     9421 ttgattacca aaacattaaa tgcaaatggt actgtcgtat ttgatcctaa tgtaagattg
     9481 ccattatggg ataacgctga agatttaaga caaactattc ataccttttt gccattggcg
     9541 catattgtaa aagtttcaga tgaagaatta gaatttatta caggtattca tgatgaaaat
     9601 gaagccattc aatcattatt tacaggtaat gttactgtag tcatttatac gaagggagca
     9661 gatggtgctg ccgtatattt gaaaaatggt attaatcatt atcatagtgg ctataaggta
     9721 aaacctgttg atacaactgg ggcaggggat gcatttattg gtgcagttat tagcaggatt
     9781 ttagcaaccg atgtgttaaa tttaactcag ttatttgaaa atgaaggtga ggaaatttta
     9841 gcgtttagca atcgcgttgc tgcaatagtg acaacgaagt atggtgctat taatagtttg
     9901 ccaacattac aagaggttga acaagcattt taaataattc atttataaaa cagtgtagga
     9961 aacaagttga atcttgtgct acactgtttt ttaaaacaaa ataagattca cggagtagga
    10021 aattatagca ttttatttat ggaaactgtc aatttcaaat ttagtatatt ttgttaaaaa
    10081 ttgcgccata ggattgtaga gtgaaataga aattgataaa ttatgtagta ataggaatag
    10141 gtatacaaag agacgttaca tcatttttta attcacgcgt catatttaat tttgttaata
    10201 aaattaaaaa gccagctata cagtgcattt gctagttatc ttgttaaaat ccaacaagat
    10261 ttacaattga atacgccgtt aactgacttt attatcttat tatatttttt taacgtttct
    10321 caccgatgca tagcagtgtt ctttattttt aaaatagaca attcgctctt tcgaatctat
    10381 agattcaata ttatggcgat tgacgacaaa gctattatga catctaaaga aacgatcatc
    10441 taattgactc agttctttta aattaccata aaattcaatt tgacggttat ctaaatgggc
    10501 aatgagtctg tgagattttg ttgatgattc aaaaaacata atatcatcat attgaacata
    10561 cactgaatta ctgccacgtt ttaattcaat cgtttcaacg ctattatctt tagacaacaa
    10621 ttgtaagcgt gtatgtgcag tttctaaaca gtctataatt cgagttctta attcagctgg
    10681 atcatcttta aaaataaaat ccatcgctgc aactttgtag acaaatgtta aataggtaag
    10741 ttcactgtga ctcgtaacga aaataatgtt accaactggg tcatgcttac gaatttcact
    10801 gcctaatttg ataccattaa tatcagttga aagttgaata tctaaaaagt aacagcctat
    10861 gtcattcata tttttagctt gctcaagcac ctcataagga ttatcagttg cgagggcaat
    10921 ttccataggc ttttcttcta tcattatata atttttaata atggtaacca tgttttctct
    10981 ttgttttgga tcgtcttcgc aaatgaaaat tttcatacat tcacatcctt atggctagtt
    11041 gttaataatt tcaacttttt gaataaagaa accattttcg ataattgtat ctaataagac
    11101 attgtctgca tt
//
LOCUS       DAXWFK010000084        10982 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.084, whole genome shotgun sequence.
ACCESSION   DAXWFK010000084 DAXWFK010000000
VERSION     DAXWFK010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10982
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.084"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(94..927)
                     /locus_tag="ACGEXS_001643"
     CDS             complement(94..927)
                     /locus_tag="ACGEXS_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFF8012652.1"
                     /translation="MEVKTFYNGNTMPQIGLGTFRVENDENCMESVKYAIEQGYRSID
                     TAKVYGNEEQVGAGIRAGLESTGIAREDLFITSKLYFEDFGRENVAAAYEASLSRLGL
                     KYLDLYLVHWPGTNEAVMVDTWKGMEDLYKNNNVKNIGVSNFEPEHLEALLAQVSIKP
                     VINQVEYHPYLTQHKLKLYLAAQRIVMESWSPLMNAQILNDETIKDIAQELGKSPAQV
                     VLRWNVQHAVVTIPKSVTPNRISENFQIFDFELSDEQMTRIDGLNQDKRIGPDPKKFE
                     G"
     gene            complement(1139..2047)
                     /locus_tag="ACGEXS_001644"
     CDS             complement(1139..2047)
                     /locus_tag="ACGEXS_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HFF8012653.1"
                     /translation="MDLSSPIVIGLIIAVVIALIFFVLFLVALGSKKKVKRQTEEKYE
                     QQEQNIKKSHEEALEKERIQNKKTITKQQEDYNHMVSTKDREIDALKLFSKNHSEYVT
                     DMRLIGIRERLVKEKRIRPEDMHIMANIFLPKDGFNNIERISHLVLTRTGLYIIDSQL
                     LKGHVYNGISGGQFKDLPPMEQVFDTLDLDKSRPQTIVMDQNDDKRSLSFVNYSDQIE
                     AIKQLAEDLQKELGAKYTPTSILYFNLKNEGDVTISNYNQNSAVKVLVGAEQLDEFFN
                     KFVFHGRIQYNVEDLQQMMDKIESFN"
     gene            complement(2172..3365)
                     /gene="metK"
                     /locus_tag="ACGEXS_001645"
     CDS             complement(2172..3365)
                     /gene="metK"
                     /locus_tag="ACGEXS_001645"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFF8012654.1"
                     /translation="MLNNKRLFTSESVTEGHPDKIADQVSDAILDAILKDDPNARVAC
                     ETTVTTGMALIAGEISTTTYVDIPKVVRETIKEIGYTRAKYGYDYETMAILTAIDEQS
                     PDIAQGVDKALEYRDKDSEEEIEATGAGDQGLMFGYATNETETYMPLAIYLSHQLAKR
                     LSDVRKDGTLNYLRPDGKVQVTVEYDENDNPVRIDTIVVSTQHAEDVTLEQIQEDIKA
                     HVIYPTVPENLINEQTKFYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGCF
                     SGKDPTKVDRSAAYAARYVAKNIVAAGLADQCEVQLAYAIGVAEPVSIAIDTFGTGKV
                     SEGQLVEAVRKHFDLRPAGIIKMLDLKQPIYKQTAAYGHFGRTDVLFPWEKLDKVEEL
                     KDAVK"
     regulatory      complement(3471..3581)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            3736..5328
                     /gene="pckA"
                     /locus_tag="ACGEXS_001646"
     CDS             3736..5328
                     /gene="pckA"
                     /locus_tag="ACGEXS_001646"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFF8012655.1"
                     /translation="MSVDTYTETTKIDKLLKKPTSHFQLSTTQLYNKILDNNEGVLTE
                     LGAVNASTGKYTGRSPKDKFFVSEPSYRDNIDWGEINQPIDEETFLKLYHKVLDYLDK
                     KDELYVFKGYAGSDKDTMLKLTVINELAWHNLFAKNMFIRPESKEEATKIKPNFTIVS
                     APHFKADPEVDGTKSETFVIISFKHKVILIGGTEYAGEMKKGIFSVMNYLLPMQDIMS
                     MHCSANVGEKGDVALFFGLSGTGKTTLSADPHRKLIGDDEHGWNKNGVFNIEGGCYAK
                     AINLSKEKEPQIFDAIKYGAILENTVVAEDGSVDFEDNRYTENTRAAYPINHIDNIVV
                     PSKAAHPNTIIFLTADAFGVIPPISKLNKDQAMYHFLSGFTSKLAGTERGVTEPEPSF
                     STCFGAPFFPLHPTVYADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMV
                     NQAISGKLKNAEYTKDSTFGLSIPVEIEDVPKTILNPINAWSDKEKYKAQAEDLIQRF
                     EKNFEKFGEKVEHIAEKGSFNK"
     gene            complement(5464..5949)
                     /gene="tnpA"
                     /locus_tag="ACGEXS_001647"
     CDS             complement(5464..5949)
                     /gene="tnpA"
                     /locus_tag="ACGEXS_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFF8012656.1"
                     /translation="MSSDTNSLAHTKWNCKYHIVFAPKYRRQVIYGKIKKDIGIILRQ
                     LCERKGVEIIEAEACKDHIHMLVSIPPKLGVSSFVGYLKGKSSLMIFDRHANLKYRYG
                     NRKFWCKGFYVDTVGRNKKVIENYIRNQLQEDIVADQISMEEYLDPFTGEKNKKRKKK
                     E"
     gene            complement(6324..7070)
                     /locus_tag="ACGEXS_001648"
     CDS             complement(6324..7070)
                     /locus_tag="ACGEXS_001648"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HFF8012657.1"
                     /translation="MPIEYFTHQFEEITYLSDDLQVKALMMTSHHEVNRIVVYLRGGK
                     GQVGRVRAGRLMQFSDSQTLVIGPYYRGNNGSEGKDEFYRGDLNDVTQLLRLLHDKYP
                     HAFIHMVGFSRGGLQGLLTFQDLPVTSYTIWGGVSDIDLMYEERVDLRGMLRRMIGHP
                     KKDRAAYEARQAIPNINENSPPILIVHGGKDQQVGIHHAYYLADQLELKGATHETFYQ
                     MAEGHVPRPPAMVETLTYIKEFMNQVESHS"
     gene            complement(7075..7548)
                     /gene="ytkD"
                     /locus_tag="ACGEXS_001649"
     CDS             complement(7075..7548)
                     /gene="ytkD"
                     /locus_tag="ACGEXS_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA deprotection pyrophosphohydrolase"
                     /protein_id="HFF8012658.1"
                     /translation="MKFRDKDNRQVNLTFKKDNEIADGNHVLAIPTFKNQLLFTKHNL
                     RGIEFPGGKRERGESSAEAVTRELYEETGAKVKNIYYIAQYTIETHDQTDFVKDVYFI
                     EVESLVSKNDYLETAGPVLFNCINDIELAQRSFLLQDSTILKCVERVQSLGFYQT"
     gene            7614..7871
                     /gene="yidD"
                     /locus_tag="ACGEXS_001650"
     CDS             7614..7871
                     /gene="yidD"
                     /locus_tag="ACGEXS_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFF8012659.1"
                     /translation="MKKIFLAMIHFYQRFISPLTPPTCRFYPTCSEYTREAIQYHGAF
                     KGLYLGIRRILKCHPLHKGGFDPVPLKKDKSASKHSHKHNH"
     gene            complement(7868..8869)
                     /gene="menC"
                     /locus_tag="ACGEXS_001651"
     CDS             complement(7868..8869)
                     /gene="menC"
                     /locus_tag="ACGEXS_001651"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFF8012660.1"
                     /translation="MKLTALHFYKYSEPFKSQIVTPKVTLTHRDCLFIELIDDKGNAY
                     FGECNAFQTDWYDHETIASVKHVIEQWFEDNRNKSFETYEAALKLVDSLENTPAARAT
                     IVMALYQMFHVLPSFSVAYGATASGLSNKQLESLKATKPTRIKLKWTPQIMHQIRVLR
                     ELDFHFQLVIDANESLDRQDFTQLQLLAREQVLYIEEPFKDISMLDEVVDGTIPPIAL
                     DEKATSLLDIINLIELYNVKVVVLKPFRLGGIDKVQTAIDTLKSHGAKVVIGGMYEYG
                     LSRYFTAMLARKGDYPGDVTPAGYYFEQDVVAHSGILKEGRLEFRPPLVDITQLQPY"
     gene            complement(8874..10352)
                     /gene="menE"
                     /locus_tag="ACGEXS_001652"
     CDS             complement(8874..10352)
                     /gene="menE"
                     /locus_tag="ACGEXS_001652"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFF8012661.1"
                     /translation="MDFWLYKQAQQNGHHIAITDGQESYTYQNLYCEASLLAKRLKAY
                     QQSRVGLYIDNSIQSIILIHACWLANIEIAMINTRLTPNEMTNQMKSIDVQLIFCTLP
                     LELRGFQIVSLDDIEFAGRDITTNSLLDNTMGIQYETSNETVVPKESPSNILNTSFNL
                     DDIASIMFTSGTTGPQKAVPQTFRNHYASAIGCKESLGFDRDTNWLSVLPIYHISGLS
                     VLLRAVIEGFTVRIVDKFNAEQILTMIKNERITHISLVPQTLNWLMQQGLHEPYNLQK
                     ILLGGAKLSATMIETALQYNLPIYNSFGMTETCSQFLTATPEMLHARPDTVGMPSANV
                     DVKIKNPNKEGHGELMIKGANVMNVYLYPTDLTGTFENGYFNTGDIAEIDHEGYVMIY
                     DRRKDLIISGGENIYPYQIETVAKQFPGISDAVCVGHPDDTWGQVPKLYFVSESDISK
                     AQLIAYLSQHLAKYKVPKHFEKVDTLPYTSTGKLQRNKLYRG"
     gene            complement(10511..10966)
                     /locus_tag="ACGEXS_001653"
     CDS             complement(10511..10966)
                     /locus_tag="ACGEXS_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4909 domain-containing protein"
                     /protein_id="HFF8012662.1"
                     /translation="MKICLMLYLLIFLSSCSTNNKIVGQRFEIQQHNETIGSIYFSAD
                     YAHIRGIEKGTAKYFIDKVGSKRYLFIEYIPDNVLNCKPDFWKTLKYKKDKVTYYVYL
                     IENLDDEVFHLSALQDMNRIPIDIADDVATMGKSPHQNDRMTLKLNKNN"
BASE COUNT         3378 a         2144 c         1665 g         3795 t
ORIGIN      
        1 taatagaaat cagctttttt acattgccta agaacttaat gtcccaagcc ctgacaattc
       61 atctaacctt ttcatcagtt gagcgatttt aatctagcct tcaaattttt ttggatcagg
      121 tccaattctc ttatcttgat ttaaaccatc aattcgcgtc atttgttcat ctgataattc
      181 gaaatcaaat atttggaaat tttcagagat tctgtttggt gtcaccgatt tagggattgt
      241 aaccacagca tgctgcacat tccatcttaa aacaacttgg gcaggtgact ttcctaattc
      301 ttgagcaatg tctttaattg tctcatcatt taaaatttgt gcattcatca atggtgacca
      361 agattccatc acgatacgtt gtgctgccaa atataatttc aatttatgtt gggttaaata
      421 tggatgatat tcaacttgat taattacagg tttaattgac acttgtgcca acaaagcttc
      481 caaatgttca ggttcaaaat tgctgacacc tatattttta acattattat ttttatataa
      541 atcttccata cctttccatg tatcaaccat tacggcttcg ttcgtacctg gccaatgtac
      601 tagatacaaa tctaagtatt ttaaacctaa tctagataaa ctagcttcgt aagcagctgc
      661 tacattttca cgaccgaaat cctcaaaata taattttgaa gtaataaata agtcttctct
      721 agcaatacca gttgactcca atccggcacg aatgccagca cctacttgtt cttcattccc
      781 ataaactttt gcggtatcaa tactacgata tccttgttca atggcatact taacactttc
      841 catgcaattt tcatcatttt ccacacgaaa tgtccctaaa ccaatttgtg gcatcgtgtt
      901 tccattataa aatgttttaa cctccataaa tatcgcctca cctttttgat gtattatacc
      961 ctgttatcat aacaaatctg agttgaatac atgagaaaaa acacttagag caatcaacca
     1021 ctaaaattct agtaatatct ctcaaatatt aatcaaattg taaaagtaat tctgtttaat
     1081 ttatgacaaa ctaaaaaagc cgaagtaaca acatatagtc atcacttcag cctaacattt
     1141 aattgaatga ttcaatttta tccatcattt gttgtaagtc ttccacgttg tattgaatac
     1201 gaccatggaa tacaaatttg ttaaagaact cgtctaattg ttcagcaccg acaagcactt
     1261 tgacagcact attttgatta taatttgaaa tcgttacatc gccttcattt ttaagattaa
     1321 agtataaaat tgaagttggt gtatatttgg cacctaattc tttttgtaag tcttcagcca
     1381 attgtttaat cgcctcaatt tgatctgaat aatttacaaa tgataatgaa cgtttgtcat
     1441 cattttgatc catcacaata gtttgcggtc tagatttatc taaatccaat gtatcaaata
     1501 cttgttccat tggtggtaaa tctttaaatt gaccgccact aataccatta taaacatgac
     1561 cttttaacaa ttgagaatca ataatataaa gaccagttct tgttaatact aaatgactaa
     1621 ttcgttcaat attattaaag ccatcctttg gtaaaaagat atttgccata atgtgcatat
     1681 cttctggtcg aattcgtttt tctttaacta atctttcacg aataccaatt aatctcatgt
     1741 ccgttacata ttcactatga tttttcgaga acaattttaa tgcgtcaatc tcacgatctt
     1801 ttgtactaac catgtgatta taatcttctt gttgttttgt aattgtcttt ttattttgaa
     1861 tacgctcttt ctctaaagct tcttcatgag actttttaat gttttgttct tgttgttcat
     1921 acttttcttc tgtttgtcgc ttaacttttt tcttactacc taaggcaact aaaaaaagga
     1981 caaaaaagat taatgcaatg actactgcaa taatgagtcc aatgactatc ggtgaagata
     2041 aatccatcac aacaacgctc ctttttaata tatgaataac tttaattata atagaaaagc
     2101 taaagatttt cgatacatat tatcatttat ataccgaaaa tcttttattt agctatattc
     2161 aattcatctt attattttac tgcgtctttt aattcttcca ctttgtctaa tttttcccat
     2221 gggaataaga catctgtacg tccaaaatga ccataagcag cagtttgttt gtaaatcggt
     2281 tgtttcaaat caagcatttt aataatacct gcaggtctta ggtcaaagtg ttttctaact
     2341 gcttcgacaa gttgcccttc agaaacttta cctgttccaa atgtatcaat tgcaattgac
     2401 actggttctg caacaccaat cgcatatgcc aattgtactt cacattgatc tgctaaacct
     2461 gctgcaacaa tatttttagc cacataacgt gcagcgtatg cagctgaacg gtctactttt
     2521 gtaggatcct taccactgaa gcatccgcca ccatgacgtg catagccacc gtacgtatca
     2581 acaatgattt tacgtcctgt taatcctgca tcaccttgag gtccaccgat tacaaagcgt
     2641 cctgtaggat tgatgtagaa tttagtttgt tcattaatca agttttctgg aacagttgga
     2701 taaatgacat gtgctttaat gtcttcttga atttgttcaa gtgtcacatc ctcagcatgt
     2761 tgtgttgata cgacaatcgt atcaatacgt actgggttat cattttcatc atattcaaca
     2821 gtgacctgaa ctttaccgtc tggtcgtaaa taatttaacg taccatcttt acgcacatct
     2881 gataaacgtt ttgccaattg atgtgataaa taaattgcta gaggcatata cgtctctgtt
     2941 tcattcgttg cgtaaccaaa cattaaacct tggtcacctg cacctgttgc ttcaatttct
     3001 tcttcgctat ctttatcacg atactctaat gctttatcca cgccttgtgc aatgtcaggt
     3061 gattgttcat caatcgcagt taaaattgcc attgtttcat aatcataacc atattttgct
     3121 cttgtgtatc caatttcttt aattgtttct ctaacaactt tcggaatatc aacatatgtt
     3181 gttgtagaaa tttcgccggc gatcaatgcc atacctgttg taacagttgt ttcacaagct
     3241 acacgtgcat ttggatcgtc ttttaaaata gcatctaata ttgcatctga cacttggtca
     3301 gcgattttat ctgggtgtcc ttctgtaaca gactctgaag taaataatcg tttgttattt
     3361 aacatagttt gctcctttaa atttatatta cgaaaattct ctctctgtga gctaaataaa
     3421 aaagaccttc taactattaa tatagagaga aggcctaata cgtccattcg ctcttatcgt
     3481 tcagacctat ttgtctgcaa acggtttggc acctttcttt tataaaaaag aggttgctgg
     3541 gtttcattgg gtccatgtcc ctccaccact caggataaga gaatccgtta aaaataatag
     3601 tacctaatta atgaattaat gtcaattttt cacaaataaa tttacagtaa aatattgtag
     3661 attaattatg ttaatgtgtt atactaatta aatgtaaagg cttacattta aattatcgct
     3721 ttggagggat ttaggatgtc agtagacaca tacactgaaa caactaaaat tgacaaatta
     3781 ctgaaaaaac caacgtcaca ttttcaactt tcgacgacac aactttataa taaaatctta
     3841 gacaataacg aaggggtatt aacagaactt ggtgctgtta atgcaagtac tggaaaatat
     3901 actggtcgtt cgcctaaaga caaatttttt gtctctgaac cttcatatag agataacatt
     3961 gattggggag aaattaatca acctatcgat gaagaaactt tcttgaagtt ataccataaa
     4021 gtactagact atttagataa aaaagatgaa ctatacgtat ttaaaggcta cgctggtagc
     4081 gataaagata caatgttaaa acttacagtc atcaatgaat tagcatggca taatttattt
     4141 gctaaaaata tgtttattag acctgaatca aaagaagaag ctacaaagat taaacctaac
     4201 ttcactatcg tttctgcacc acattttaaa gcagatccag aagttgatgg tactaaatct
     4261 gaaacctttg tcattatttc atttaaacac aaagtcattt taatcggcgg tactgaatac
     4321 gctggtgaaa tgaaaaaagg tatcttctct gtaatgaatt atctcttacc gatgcaagat
     4381 attatgagca tgcattgctc agcaaacgtt ggtgaaaaag gcgatgttgc attattcttt
     4441 ggtctatctg gcactggtaa aacaacctta tcggctgacc cacaccgtaa actaatcggt
     4501 gatgatgaac acggctggaa taaaaacggg gtctttaata tcgaaggtgg ctgctatgca
     4561 aaagcaatta atctttccaa agaaaaagaa ccacagattt ttgacgcaat caaatatggt
     4621 gcaattttag agaacactgt agttgcagaa gatggttcag tggactttga agacaatcgt
     4681 tatacagaaa acacgcgtgc cgcttatcca attaatcaca ttgacaatat tgtagtacca
     4741 tctaaagcag cacatccaaa tacaattatt ttcttaactg cggatgcatt tggtgttatt
     4801 ccaccgattt caaagttaaa taaagaccaa gcaatgtatc atttcttgag tggtttcact
     4861 tctaaattag ctggtacaga gcgtggtgtg acagaacctg aaccatcatt ctcaacatgt
     4921 ttcggagcac cgttcttccc gttacaccct actgtttacg ctgatctatt aggtgaactt
     4981 atcgatttac atgatgttga tgtttatctt gttaatactg gatggactgg cggaaaatat
     5041 ggtgtaggac gtagaatcag cttacattac acacgtcaaa tggtaaacca agcgatttct
     5101 ggcaaattga aaaatgcaga atatacaaaa gatagtacgt ttggtttaag cattcctgta
     5161 gaaattgaag atgtaccgaa aacaatttta aatccaatta atgcttggag cgacaaagag
     5221 aaatataaag cacaagcaga agatttaatt caacgttttg aaaagaactt cgaaaaattt
     5281 ggtgaaaaag ttgaacatat tgctgaaaaa ggtagcttca acaaataaat ttgaatacta
     5341 aatcaaaacc acccgtgtga acgggtggtt tgttctgcgg ctataagcct tccttactgg
     5401 ccagccctaa aagggcactg acaagtcagc caactgcact actattccag caaccctaaa
     5461 gggttactct tttttctttc tttttttatt tttctctcca gtgaaaggat ctaaatattc
     5521 ttccattgag atttggtctg caacgatatc ctcttgtaat tgattacgaa tataattttc
     5581 aatcactttt ttatttctac ctactgtatc cacataaaat cctttacacc aaaactttct
     5641 atttccatat ctatacttta agttagcatg tctatcaaat atcattaaac tactttttcc
     5701 ttttaaatag ccaacaaatg atgatacccc aagtttgggt ggtatactaa ctaacatatg
     5761 gatatgatct ttacatgcct ctgcttcaat tatctctaca ccttttcttt cacataattg
     5821 acgcaatata atccctatat ctttttttat ttttccatat atcacttgtc ttctgtattt
     5881 aggtgcaaag acaatatgat acttacaatt ccattttgta tgtgctaaac tgtttgtgtc
     5941 agatgacatt aaatagcatc tcctcgtgtt gattattttg gttggctgac caatatttat
     6001 tctagcacgt agagatgcat tttttgtgac aatggtagaa ccttttctga accatacgca
     6061 tagcgtatgg ttttcttttt acaattaaag agccaaccgt tgttatagtc taacaatggt
     6121 tggctcctct tattttatgt gctaaaaatt tataggcaat tttattacaa caatgtacat
     6181 ttaaggtgac cttcatgcca aaatcgcatc actcatttaa tggaagcagc acgtcttcat
     6241 ataaagtacc gatccctaat tcaacgcatg tagtaccaca tcttcaaagc ttgatagttc
     6301 ccatgcgcac accacgtttc atactagcta tgcgactcaa cttggttcat aaactcttta
     6361 atataagtca atgtttcaac catcgctggt ggtcttggca catgtccttc tgccatttga
     6421 taaaatgttt catgcgtggc accttttaac tctagttggt ccgctaaata atacgcatga
     6481 tgaataccaa cttgctggtc tttccctcca tgtacaatta atattggcgg actgttttca
     6541 ttaatgtttg gaatcgcttg gcgtgcctca tatgccgctc gatctttttt cggatgacca
     6601 atcattcttc gtagcatgcc tcttaaatcg acacgttctt catacattaa atcaatatct
     6661 gagacaccac cccagattgt ataacttgtt actggtaagt cttgaaatgt caacaatcct
     6721 tgtaaaccac ctcgcgaaaa accaaccatg tggataaatg catgtggata tttatcatgt
     6781 agcaacctta ataattgcgt cacatcattt aaatcgccac ggtaaaattc gtctttgcct
     6841 tcactcccat tgttacctcg gtagtatggc ccaatcacta aagtttgact atctgaaaat
     6901 tgcattaatc tacctgcgcg cacacgtcct acttgacctt tgccacctcg caaataaact
     6961 acaatgcgat ttacttcatg atgtgatgtc atcattaaag cttttacttg taagtcatct
     7021 gacaaatatg taatttcttc gaattgatgc gtaaaatatt caattggcat tcgtttacgt
     7081 ttgataaaac ccaagtgatt gcaccctctc tacgcatttt aaaatggtac tatcttgcag
     7141 taagaaactc cgttgtgcga gttcaatatc attgatacag ttaaacaaca ctggccctgc
     7201 tgtttctaaa taatcgttct tgcttaccaa tgattcaact tcgataaaat atacatcttt
     7261 tacaaaatca gtttgatcat gtgtttcaat ggtatattgt gctatgtaat aaatattttt
     7321 aactttggcg cctgtttctt catataattc acgtgtaact gcttcagcac tactttcccc
     7381 gcgttccctt ttaccaccag gaaattcaat cccccgtaaa ttatgtttgg taaaaagcaa
     7441 ttgattttta aacgttggaa tagctagcac atgattgcca tctgctatct cattatcctt
     7501 tttaaatgtc aaattaactt gacgattatc tttatcccta aacttcacgc gcatcacatc
     7561 cctacattgt atgttaatat aatagttaat tactatcgtt ggaggcatta attatgaaaa
     7621 agatattctt ggcgatgatt catttttatc aacgtttcat ttcgccactc actccaccaa
     7681 cttgtcgttt ttatccaaca tgttcagagt acactagaga agcgattcaa taccacggtg
     7741 ctttcaaagg cctttattta ggtatccgtc gtattttaaa atgtcatccg cttcataaag
     7801 gcggctttga ccctgttccg ttaaaaaaag acaagtcagc aagcaagcat tcacataaac
     7861 ataaccatta atatggttgt aattgagtta tatccactaa aggggggcga aattcgagtc
     7921 gcccctcttt taatatgcct gaatgcgcca ccacatcttg ttcaaaataa taacctgctg
     7981 gtgtaacatc tcctggataa tcacctttac gagcaagcat cgctgtaaaa tagcggctta
     8041 aaccatattc gtacatgccg ccaataacca cttttgcacc atgacttttc aaagtatcaa
     8101 ttgccgtttg cactttatca atgccaccta gacgaaatgg ttttaataca acaactttca
     8161 cattgtataa ttctatcaaa ttaattatgt ccaacaacga tgttgccttt tcatcaaggg
     8221 ctattggagg tattgttcca tccactactt catcaagcat ggagatatct ttaaatggct
     8281 cttcgatata aagaacctgt tcacgcgcta ataactgtaa ctgtgtgaaa tcttgacgat
     8341 ccaaggactc atttgcatct ataaccaatt gaaagtgaaa gtctaattcc cgtaacactc
     8401 taatttgatg catgatttga ggcgtccatt ttaatttaat tctggtcggc tttgttgctt
     8461 ttaatgactc tagttgttta tttgataagc cgctcgctgt cgctccatat gctactgaaa
     8521 atgaaggcag tacatgaaac atttgataca atgccatgac aatagttgcc cttgcagcag
     8581 gcgtattttc caatgaatct actaatttta gtgctgcttc atacgtttca aatgatttat
     8641 ttctattatc ttcgaaccat tgctcaatta catgtttcac tgaggcaatt gtttcatgat
     8701 cataccaatc tgtttgaaaa gcgttacatt ccccgaaata tgcatttcct ttgtcatcaa
     8761 tcaattcgat aaacaaacaa tcacgatgcg ttaaagtgac tttcggtgtt acaatttgtg
     8821 acttaaatgg ctcactatat ttataaaaat gcaaagctgt caacttcatc aaatcatcct
     8881 ctatacaact tatttctttg taatttacct gttgatgtat aaggtaaagt atcaaccttt
     8941 tcaaagtgtt tcggtacttt atatttcgct aaatgttgtg ataaatatgc aatcaattgt
     9001 gcctttgaaa tgtcactttc actgacaaaa tataatttag gcacttggcc ccaagtatca
     9061 tcaggatgcc ctacacatac tgcgtcactg atacctggaa attgcttcgc taccgtttca
     9121 atttgatatg gataaatatt ttcaccgcca ctaataatta aatctttacg tcggtcataa
     9181 atcatgacat aaccttcatg atctatttca gcaatgtcac ccgtattaaa ataaccattt
     9241 tcaaacgtac ccgttaaatc tgttggatac aaatatacat tcatcacatt ggcgccttta
     9301 atcattaatt ctccatgacc ttctttatta ggatttttaa tttttacgtc aacattggca
     9361 cttggcatcc ctacagtgtc aggacgtgca tgcaacattt ccggtgttgc tgttaaaaat
     9421 tgcgaacatg tctcagtcat accaaatgaa ttataaattg gcaggttata ttgtaatgcc
     9481 gtctctatca tagtggcaga taatttagca ccgccgagta atattttttg taaattataa
     9541 ggttcatgta aaccttgttg cataagccaa tttaaagttt gtggcacaag cgaaatgtgc
     9601 gtgattcgtt catttttaat catcgttaaa atttgttcgg cattgaattt atcaacaatg
     9661 cgcacagtaa aaccttcaat aacagctctt aaaagtacac tgagacccga aatatgataa
     9721 atcggcaaga cagatagcca attagtgtca cgatcaaatc ccaagctctc tttacatccg
     9781 attgcactgg cataatgatt acgaaacgtt tgtggcaccg ctttttgagg gcccgttgtc
     9841 cctgatgtaa acataatcga tgcaatgtca tctaaattaa atgatgtatt taatatgttg
     9901 gacggcgact ctttcggcac cacagtttca ttcgatgttt catattggat acccattgtg
     9961 ttgtccaaca aactgttcgt tgtaatatcc cttccagcga attcaatatc atccagcgat
    10021 acaatttgaa accctcgtaa ttccagtggc aaggtacaaa aaatcaattg tacatcgatt
    10081 gacttcatct gattcgtcat ctcattaggt gtcaaccttg tattaatcat cgcaatttca
    10141 atatttgcca accaacatgc atgtattaaa atgatcgatt gaatcgaatt atctatgtat
    10201 agcccaacac gagattgttg ataagccttg agtcttttag ccaatagact cgcttcacag
    10261 tataaatttt gataagtata agattcttga ccgtctgtta tcgcaatatg atgtccattt
    10321 tgttgtgctt gtttatataa ccaaaagtcc atgcgttatt cctccaaaat catttacatt
    10381 ataattataa cgattttatg acattctagc agtggttatg tttaaaaata taaaaaagta
    10441 gacgaattga tgcattgata tgattgttat aatgctcaat acatatcgtt atatcattcg
    10501 tctactatta tcagttattt ttatttaatt ttagtgtcat tctgtcattt tgatgtggtg
    10561 atttacccat tgttgccaca tcatctgcaa tgtcaattgg tatacggttc atgtcttgta
    10621 atgcacttaa atggaatact tcatcatcta aattttcaat gagatataca taatatgtta
    10681 ccttgtcctt tttatatttt aacgttttcc aaaagtccgg cttgcaattc aatacattat
    10741 ccggaatata ttcaataaat aagtaacgtt tgctgcctac tttgtctatg aaatattttg
    10801 cagtgccttt ttctatacct cttatatgtg catagtctgc tgaaaagtaa atactaccta
    10861 ttgtttcatt atgttgttgt atttcaaatc gttggcctac tattttatta tttgtgctac
    10921 aactacttaa aaaaatcagt agatatagca ttaaacatat tttcatcccc ttgaatttta
    10981 aa
//
LOCUS       DAXWFK010000085        10966 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.085, whole genome shotgun sequence.
ACCESSION   DAXWFK010000085 DAXWFK010000000
VERSION     DAXWFK010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10966
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.085"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            32..748
                     /locus_tag="ACGEXS_001654"
     CDS             32..748
                     /locus_tag="ACGEXS_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8012663.1"
                     /translation="MGRKWNNIKEKKAQKDKNTSRIYAKFGKEIYVAAKSGEPNPESN
                     QALRLVLERAKTYSVPNHIIEKAIDKAKGAGDENFDHLRYEGFGPSGSMLIVDALTNN
                     VNRTASDVRAAFGKNGGNMGVSGSVAYMFDHVATFGIEGKSVDEILETLMEQDVDVND
                     VIDDNGLTIVYAEPDQFAVVQDALRAAGVEEFKVAEFEMLPQTDIELSEADQVTFEKL
                     IDALEDLEDVQNVFHNVDLK"
     gene            748..1221
                     /locus_tag="ACGEXS_001655"
     CDS             748..1221
                     /locus_tag="ACGEXS_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFF8012664.1"
                     /translation="MKSAEQWIDELQLESHPEGGFYRETIREVLKDGRRAPFSSIYFL
                     LTDDNISHFHRIDADEVWYYHAGDSLTIHMINPDGEYTTATLGTDIQNGDVLQYVVPK
                     GTIFASSIEISNTFSLVGCMCQPAFEFKQFELFKQSELITQYPHLKSVIEKYALK"
     gene            1313..1429
                     /locus_tag="ACGEXS_001656"
     CDS             1313..1429
                     /locus_tag="ACGEXS_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012665.1"
                     /translation="MLQLFLMKIALNDFENDKSVLFIFLKNHFYEEMCVLSN"
     gene            1574..2218
                     /locus_tag="ACGEXS_001657"
     CDS             1574..2218
                     /locus_tag="ACGEXS_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFF8012666.1"
                     /translation="MKVKYIDKRHWRRLIDREYTEVKVNNNRFKGIIGLVTMKKVRDP
                     LEVTVVGQNIIVADDNYKWLQILPEKKRYSITVMFDNKGNPLEYYFDINIKNITQKGN
                     ARTVDLCLDVLALPSGEYELVDEDDLMFALESEQITKKQFHEAYMIAHQIMAELENDF
                     KGFQKKIMYCFNKINAKAQKNHQKPQNKTNIEKSKQIKPKQYNQTKNHQQQKKN"
     gene            2334..3200
                     /locus_tag="ACGEXS_001658"
     CDS             2334..3200
                     /locus_tag="ACGEXS_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFF8012667.1"
                     /translation="MKIEDYRLLITLDETKTLRKAAEILYISQPAVTQRLKAIENAFG
                     VDIFIRTKKQLITTTEGTMIIEHARDMLKRERLFFDKMQAHIGEVNGTISIGCSSLIG
                     QTLLPEVLSLYNAQFPNVEIQVQVGSTEQIKANHRDYHVMITRGNKVMNLANTHLFND
                     DHYFIFPKNRRDDVTKLPFIEFQADPIYINQIKEWYNDNLEQDYHATITVDQVATCKE
                     MLISGVGVTILPEIMMKNISKEQFEFEKVEIDNEPLIRSTFMSYDPSMLQLPQVDSFV
                     NLMASFVEQPKA"
     gene            3401..4621
                     /locus_tag="ACGEXS_001659"
     CDS             3401..4621
                     /locus_tag="ACGEXS_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HFF8012668.1"
                     /translation="MDMFAALLQIKNYKLFVANMFLLGMGIAVTVPYLVLFATKDLGM
                     TTNQYGLLLASAAISQFTVNSIIARFSDTHHFNRKIIIILALLMGALGFSIYFFVDTI
                     WLFILLYAIFQGLFAPAMPQLYASARESINVSSSKDRAQFANTVLRSMFSLGFLFGPF
                     IGAQLIGLKGYAGLFGGTISIILFTLVLQVFFYKDLNIKHPISTQQHVEKIAPNMFKD
                     KTLLLPFIAFILLHIGQWMYTMNMPLFVTDYLKENEQHVGYLASLCAGLEVPFMIILG
                     VLSSRLQTRTLLIYGAIFGGLFYFSIGVFKNFYMMLAGQVFLAIFLAVLLGIGISYFQ
                     DILPDFPGYASTLFSNAMVIGQLGGNLLGGAMSHWVGLENVFFVSAASIMLGMILIFF
                     TKNQKITKEDVIST"
     gene            4618..5106
                     /locus_tag="ACGEXS_001660"
     CDS             4618..5106
                     /locus_tag="ACGEXS_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HFF8012669.1"
                     /translation="MTIILWLLIIAAFMLAFVGLIKPIIPSVLVLWVGFLIYQFGFHN
                     QHLSWVFYVSMALLTILILCADFLANKYFVNRFGGSKFGEYAALIGVVIGCFVLPPFG
                     IIIIPFILVFIVELIQGYSFERAVKVSIGSIVAFLTSSIAQAIIMFIMIVWFFIDALL
                     IN"
     gene            complement(5205..5894)
                     /locus_tag="ACGEXS_001661"
     CDS             complement(5205..5894)
                     /locus_tag="ACGEXS_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 family protein"
                     /protein_id="HFF8012670.1"
                     /translation="MKSTAQLTKENNVKSLRLSNTDREIFENYMTYMRSDFRVNPHDT
                     ELIINRILKQLLSAEQHGLLALDFFNHDPKAHAIKELKAMPNETFKNIFKYIYQHIVL
                     LIGIVSFLKGFLGFFMEKNGSNLYFVSFPFSVVVGFFIVFLFIWFSFKTIQLQCFNNS
                     NWIWIFTYLAIILLIVGFFYVFFIPQSLLAFGPYIQVSNWVFIIFSFIVMPIGLRIER
                     NISKKHSHTFL"
     gene            6021..6464
                     /locus_tag="ACGEXS_001662"
     CDS             6021..6464
                     /locus_tag="ACGEXS_001662"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8012671.1"
                     /translation="MRTLNKDEHNYIKQIANIHETLLSQVESNYKCTKLSIALRYEMI
                     CSRLEHTNDKIYIYENEGQLIAFIWGHFSNEKSMVNIELLYVEPQFRKLGIATQLKIA
                     LGKWAKTMNAKRISNTIHKNNLPMISLNKDLGYQVSHVKMYKDID"
     gene            6531..6926
                     /locus_tag="ACGEXS_001663"
     CDS             6531..6926
                     /locus_tag="ACGEXS_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012672.1"
                     /translation="MKKLIISIMAVMLFLTGCGKSQEKATLEKDIDNLQKENKELKDK
                     KEKLQQEKEKLADKQKDLEKEVKDLKPSKEDNKDDKKDEDKNKDKDKDKEASQDKQSK
                     DQTKSSDKDNHKKPTSADKDQKANDKHQS"
     gene            7064..7363
                     /locus_tag="ACGEXS_001664"
     CDS             7064..7363
                     /locus_tag="ACGEXS_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012673.1"
                     /translation="MHEQDFRILEGQDITLPELGRELENITGHTIADSTGEIKRVIAH
                     LPNFESDTDTFVATYRLNHQQDFIDATFTALKSDRARLKEVPVHVELISYISKSK"
     gene            7443..7985
                     /locus_tag="ACGEXS_001665"
     CDS             7443..7985
                     /locus_tag="ACGEXS_001665"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8012674.1"
                     /translation="MIYCETERLILRDWHEDDLLPFQKMNANYDVRKYFPSLLSYRRS
                     ELDMRTMDAVIKDYGIGLFAVEDKESHQWIGFIGLNYIPETSDYPFKELPLYEIGWRL
                     LPEFWGKGLATEGAKATLKLAEEHQIYDVYSFTAEANKASQRVMEKIGMTVYDHFELP
                     NLSKYHLLKRQVRYYINLRK"
     gene            complement(8081..8647)
                     /locus_tag="ACGEXS_001666"
     CDS             complement(8081..8647)
                     /locus_tag="ACGEXS_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFF8012675.1"
                     /translation="MKRIAVYCGASKGHDPSYVQKAYDLGKYFAEQGYELVFGAGSIG
                     IMGAIQDGVLDHGGKAIGVMPKMLDEHEITSQRLTELILVDSMHERKNKMAELADAFV
                     MAPGGAGSLEEFFEMYSWAQIGIHEKPIAIYNINGFFNPLQTMIDHMIEEGFIDPKYR
                     ALAPLCDTKESLIESILNFKPLGTRSYD"
     gene            complement(8649..9107)
                     /locus_tag="ACGEXS_001667"
     CDS             complement(8649..9107)
                     /locus_tag="ACGEXS_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFF8012676.1"
                     /translation="MTHIIIDGDACPVVDSIIDLTTETGIFVTIIRSFSHFSNQLYPP
                     HVSTLYVDDGPDAVDYKIVQLSTKDDIVVTQDYGLASLLVDKVLIVMHHNGKIYNSKN
                     IQQLLDKRYMNAQIRKQGGRHKGPPPFTKQDQKVFEQSLLKVIHRIKELD"
     gene            complement(9110..9793)
                     /locus_tag="ACGEXS_001668"
     CDS             complement(9110..9793)
                     /locus_tag="ACGEXS_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012677.1"
                     /translation="MDKKKVIKFMINVLPIVLVPLIVERKRIKQHPDVQKVTDATSKV
                     ASKTSAAISNTASDVKEYVGDKKQDFENKRELKKFAREHDPAYIEKKGEKLAKQNRKD
                     ADKMNKILQKNIEKRHKEEQKAREKNEIQRIKDMKKSQKYEEKAGLTPNKLDEKTEKK
                     GDKLAEKNRKEIAKMNKKLQKNIEKRHKEEQKRQQEADKARIKSFKKYKDYVAKSASQ
                     QNKENNTEA"
     gene            complement(9965..10840)
                     /locus_tag="ACGEXS_001669"
     CDS             complement(9965..10840)
                     /locus_tag="ACGEXS_001669"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFF8012678.1"
                     /translation="MFIIELIKGIILGVVEGLTEFAPVSSTGHMILVDDMWLKSSEFL
                     GSQSAFTFKIVIQLGSVFAAAWVFRERFLEILHIGKHKHVEGDNDQQRRSKPRRLNLL
                     HVLVGMVPAGILGLLFDDFIEEHLFSVPTVMIGLFVGAIYMIIADKYSAKVKNPQTVD
                     QISYFQAFVIGISQAVAMWPGFSRSGSTISTGVLMKLNHKAASDFTFIMAVPIMLAAS
                     GLSLLKHYQDIQIADIPFYILGFLAAFTVGLIAIKTFLHLINKIKLIPFAIYRIVLVI
                     FIAILYFGFGIGKGI"
BASE COUNT         3835 a         1552 c         1898 g         3681 t
ORIGIN      
        1 taattaaaca gttcgaaagc gagatgacat tatgggacgt aaatggaata acattaaaga
       61 aaaaaaggcc caaaaagata aaaacacaag tagaatatat gcgaaatttg gtaaggagat
      121 ttatgttgca gcaaaatctg gtgaacccaa tccagaatct aaccaagctt taaggttggt
      181 gcttgaacgc gctaagacat attcagtgcc gaatcatatt attgaaaaag caatagataa
      241 agctaagggt gctggagacg aaaactttga tcacctaaga tatgaaggat ttggcccaag
      301 cggatcaatg ctaattgttg atgcgttaac aaataatgta aatcgtactg cctctgatgt
      361 gcgagctgct tttggtaaaa acggcggtaa tatgggtgta tctggatcag ttgcttatat
      421 gtttgatcat gtggcaacat ttggtattga aggaaagtct gttgacgaaa tacttgaaac
      481 attaatggaa caagatgtag atgtaaatga tgtgattgac gataatggat tgacaatagt
      541 ctatgctgaa ccagatcaat ttgcagtcgt tcaagatgcg cttcgtgcag caggtgttga
      601 agaatttaaa gttgctgaat ttgaaatgtt acctcaaaca gatattgaac tttctgaagc
      661 ggaccaagta acatttgaaa aattaatcga tgcattagaa gatttagaag atgtacaaaa
      721 cgtattccat aatgtggatt tgaaataatg aaatcagcag aacaatggat tgatgaattg
      781 caacttgaat cacatcctga aggtggtttc tatagagaga caattcgaga agtattgaaa
      841 gatggacgca gagcgccgtt tagtagtatt tattttttac ttacagatga caatatttcg
      901 cattttcatc gaattgatgc tgatgaagta tggtactatc atgctggcga ttctctaaca
      961 attcatatga taaatccgga tggggaatat acgactgcaa cattgggtac tgatatccaa
     1021 aatggagatg tattgcaata tgtagtgcct aaaggaacaa tttttgcttc ttcaatcgaa
     1081 atatcaaata cttttagttt agtaggttgt atgtgtcaac cggcatttga gtttaagcag
     1141 tttgaattgt ttaagcaatc tgaattaatt acgcaatatc cgcatcttaa atcagtaatc
     1201 gaaaaatatg ctttaaaata aaagtgatca atgaagtggt ttgaaggttg ttaataaacc
     1261 tttgagtcac ttcattttta tatgtattct tgattgaatc agaatagatt tgatgcttca
     1321 gctgttttta atgaaaatag cattaaatga ttttgaaaac gataagagtg tgttatttat
     1381 atttttgaaa aatcactttt atgaagaaat gtgtgtacta tctaattaat attgtctatt
     1441 ttaagtaaat tgaagcgagt tgtagtaaaa taataataaa tattttcata tgattaacaa
     1501 aaactataaa actgtatcat gacatgacat catttatgta gttttatata ataatgacaa
     1561 ggagttggtg actgtgaaag taaagtatat agataaacgt cactggcgtc gcctaattga
     1621 tagggaatac acagaggtaa aagttaataa taataggttt aagggtatta taggcttagt
     1681 cacgatgaaa aaggttcgtg atcctttaga ggtgacggta gttggacaaa atatcattgt
     1741 cgcagatgac aattataaat ggttgcaaat actacctgaa aagaaacgtt atagtataac
     1801 tgtaatgttt gataataaag gcaatccatt agaatattat tttgatataa atatcaaaaa
     1861 tataacgcaa aaaggtaatg cgcgtacagt agatttatgt ttagatgttt tagcattacc
     1921 aagtggtgaa tatgagttgg tagatgaaga tgatttaatg tttgcattag aaagtgagca
     1981 aattacaaaa aagcaatttc atgaagcata tatgattgca catcaaatta tggcagagtt
     2041 agaaaatgat tttaaaggat tccaaaagaa aatcatgtac tgctttaata aaattaatgc
     2101 aaaggctcaa aaaaatcatc aaaagccaca aaataaaact aatattgaaa aaagcaaaca
     2161 aataaagcct aagcaatata atcaaactaa aaatcaccaa caacaaaaga aaaactaagt
     2221 aattcaagct gcagccatac caataaaatt ggtgaaatta ttcgttagta atgaactcat
     2281 gaatgcttgt agccatgaaa gttcaataat tgaataattt atggggggaa attatgaaga
     2341 ttgaagacta tcgtttacta ataacattag acgaaacgaa aacgttacgt aaagcggctg
     2401 aaattttata tatatctcaa cctgctgtta cacaaagact aaaagctatt gaaaatgctt
     2461 ttggagtaga tatttttatc agaacaaaaa aacaattgat tacaacaact gaaggaacaa
     2521 tgattattga gcatgctcgt gacatgttga aaagagagcg attatttttt gacaaaatgc
     2581 aggcacatat tggtgaagtg aatggaacaa tatcaatcgg gtgttcttct ttgattggac
     2641 aaaccttact tcctgaagtt ttgagcctat ataatgccca atttcctaat gttgaaatac
     2701 aagtgcaagt tggttcaact gaacaaatta aagcaaatca tagagattat catgttatga
     2761 taactcgtgg aaataaggta atgaatttag ctaacacaca tttatttaat gatgatcatt
     2821 attttatttt tccaaaaaat agacgagatg atgttacaaa gttaccattt atagagtttc
     2881 aagctgatcc gatttatata aatcaaataa aagaatggta taacgataat ttagaacaag
     2941 attaccatgc aactattaca gtggatcaag tagcaacttg caaagaaatg ttgattagtg
     3001 gtgtaggtgt tacaatcttg ccagaaatta tgatgaaaaa tatcagcaaa gaacaatttg
     3061 agtttgaaaa agtagaaatt gataatgaac cgctgattcg ttcgacattt atgagttatg
     3121 atccgagcat gttgcaattg ccacaagttg attcttttgt aaatctcatg gcgagctttg
     3181 ttgaacaacc aaaggcgtag ttttagacta atttaaggtt tgtatttaat tttaaactat
     3241 tcggttaaat tgaacgtagt tggttgctaa tgcaccaaca gcaaaagagc ccctaattaa
     3301 taaattaaaa ggggacaaag gaatacagtt ggatgctaac gcaccaactg cataagagcc
     3361 cctaattaat aaattaaaag gggctctaag aagcggggtt atggatatgt ttgcagcgtt
     3421 attacaaata aagaattata aactctttgt tgctaatatg tttctactag gtatgggtat
     3481 tgcggttacg gtcccatatc ttgttctttt tgcaactaaa gatttaggta tgacaacaaa
     3541 tcagtatgga ttacttctag catctgcagc gattagccag tttacagtaa attcaattat
     3601 tgctagattt tcggatacgc atcactttaa tagaaaaatt attattattc tcgcattatt
     3661 aatgggtgcg cttggttttt caatatactt ttttgtagat acaatctggt tattcatatt
     3721 actatatgcg attttccaag gattatttgc accagcaatg ccccaacttt acgcatctgc
     3781 tagagaatct atcaatgttt caagctctaa agatagagct caatttgcca acacagtatt
     3841 acgttcaatg ttctcattgg gctttttatt tggtccattt attggtgccc aattaatcgg
     3901 attaaaaggc tatgctggat tgtttggtgg aacaataagt atcattttat ttactttagt
     3961 acttcaagtg tttttctata aggatttaaa cattaaacac cctattagta cgcaacaaca
     4021 tgttgaaaaa attgctccta atatgtttaa agacaaaacg cttttattac catttattgc
     4081 atttatttta ttacacattg gacaatggat gtatacgatg aatatgcctt tatttgttac
     4141 tgattattta aaagaaaatg aacaacatgt cggttattta gctagtttat gtgctggttt
     4201 agaagtgcca tttatgatca ttcttggcgt tttatcatct agattacaga ctcgaacatt
     4261 gttgatttat ggagcgattt ttggtggttt attctacttc agcattgggg tatttaaaaa
     4321 cttctatatg atgttagcag gacaggtgtt tttagctatt ttcttagcgg ttcttttagg
     4381 aattggtatt agttatttcc aagatatctt accagatttt ccaggatacg cttcaacact
     4441 attttctaat gcaatggtta ttggacagtt aggcggtaac ctattaggtg gtgctatgag
     4501 tcactgggta ggtttggaaa atgtattttt tgtatcagca gcatcaatca tgttaggtat
     4561 gatacttata ttctttacta aaaatcaaaa aattacaaaa gaggatgtga tatcaacatg
     4621 acaattattt tatggctact tatcatcgct gccttcatgt tagcatttgt tgggttgatt
     4681 aagccgatta ttccttctgt tttagtatta tgggttggct ttttaatcta tcaatttggc
     4741 tttcataatc agcatttatc atgggtgttt tatgtatcta tggcattgct aacaatatta
     4801 attttatgtg ccgacttttt agctaataaa tattttgtga atcgcttcgg tggttctaag
     4861 tttggagagt atgcagcttt aattggtgtg gttattggat gttttgtttt accgccattt
     4921 ggaattatta ttataccttt tattttggta ttcatagttg aattaataca aggctattca
     4981 tttgaaagag cagttaaagt aagtataggt tcaatcgtag catttttaac aagtagtata
     5041 gctcaagcaa tcattatgtt tataatgatt gtatggttct ttatagatgc tttattgatt
     5101 aattaataaa aagcttattg caaaatatgt ttttcggtaa ctgtaattta gtgattttat
     5161 cattaacagt accaaattcg catatgatgt aataagcttt ttgtttataa aaatgtatga
     5221 gaatgttttt tcgaaatatt tctttcaatg cgtaatccaa taggcataac tataaatgaa
     5281 aatataataa atacccaatt gcttacctga atatatggtc caaaggcaag taatgattgt
     5341 ggaataaaga atacatagaa aaatcctaca attagtagaa tgattgctaa ataggtgaat
     5401 atccatatcc aatttgaatt gttaaaacac tgcagttgta ttgttttaaa actaaaccat
     5461 ataaataaaa agacaataaa gaatccaacg actactgaaa acgggaatga aacaaaatat
     5521 aaattacttc catttttttc catgaaaaat cctaaaaatc ctttgagaaa actaacaatc
     5581 ccaattaata gaacgatgtg ttgatagata tatttaaaaa tatttttaaa tgtttcatta
     5641 ggcatcgctt ttagttcttt tattgcatgt gcttttgggt cgtgattgaa aaaatctaag
     5701 gctaataaac catgttgttc tgcgcttaat aattgtttga gtatacggtt aataattaac
     5761 tctgtatcat gagggttgac gcgaaagtca gagcgcatat aagtcatata attctcgaag
     5821 atttctctat cagtattgct taatcttaat gatttaacat tattttcttt tgttaattgc
     5881 gcagtacttt tcattgttac ttaagcgctc ctttaaaaat gtttaattcc aaattaaaat
     5941 ggaaatgatt ttatagtatt aataaggtca atcatatcat attaaacgca taaatataac
     6001 gattaatatt ggagaggaaa atgaggacac ttaataaaga tgaacataat tatatcaagc
     6061 aaatagctaa tatacatgag acattattgt cgcaagtaga atccaactat aaatgtacta
     6121 aactgagtat tgctcttagg tacgagatga tatgttcaag attagaacat acaaatgata
     6181 aaatttatat atatgaaaat gaaggtcaat taatagcgtt tatttgggga cattttagta
     6241 atgaaaaaag tatggttaac attgaactgc tatatgttga accacaattt cgcaaactgg
     6301 gaatagctac gcaactgaag attgcgcttg gaaaatgggc aaaaactatg aatgcaaagc
     6361 gaataagcaa tacaattcat aaaaataatt tgccaatgat atctttgaat aaagatttag
     6421 gttatcaagt gagtcatgtg aaaatgtata aagatattga ttagaattag gattatgttg
     6481 ctaattcatg ttaaaattaa aaaagattta atgacgttaa ggagttttat atgaagaaat
     6541 taatcatcag tattatggcg gtcatgctat ttttaacagg ttgtggtaaa agtcaagaga
     6601 aagccactct ggaaaaggat atcgataatt tacaaaaaga aaataaagaa ttaaaagaca
     6661 aaaaagaaaa gcttcaacaa gaaaaagaaa aattagcaga taagcaaaaa gaccttgaaa
     6721 aagaagtgaa agatttaaaa ccttcaaaag aagataacaa ggatgataaa aaagacgaag
     6781 acaaaaataa agacaaagat aaagataaag aggcatcaca agataagcaa tcaaaagatc
     6841 aaactaagtc atcggataaa gataatcaca aaaagcctac atcagcagat aaagatcaaa
     6901 aagctaatga caaacaccaa tcataatcga attgcttact tgttatagat gaaaggtaca
     6961 gcgttttaaa ccttatttta agggtatgta ttaattaaaa tgtggtcatg attgaaacag
     7021 aacgtaaaaa tagacaacgt aattaataaa ggagagaaac ggcatgcatg aacaagattt
     7081 tagaatttta gagggtcaag atattacttt gccagaatta ggtagagaat tagaaaatat
     7141 tacaggacat acgattgctg attctactgg cgaaattaag cgtgtaattg cacatttacc
     7201 aaactttgag tccgatacag atacttttgt tgctacatat cgtttaaacc atcaacaaga
     7261 ttttatagat gcaactttta ctgcgctgaa atcagataga gcacgtttaa aagaagtgcc
     7321 agttcatgtt gaacttataa gttatatttc taaatcaaaa taaactgcta tctaaaacgc
     7381 aaagttgatc aaaatatcga ttttgtgttt tttattgaga aattatatag gagtgtcaat
     7441 cgatgattta ttgtgaaaca gagcgtttaa tattaagaga ctggcatgaa gatgatctgt
     7501 taccttttca aaaaatgaat gcgaattatg acgtacgtaa atattttcca agtttattga
     7561 gttatcgtcg ttcagaatta gatatgagaa ctatggatgc ggttattaaa gattatggca
     7621 ttggattatt tgctgtagaa gataaagagt cccatcaatg gataggcttt ataggtttga
     7681 attatattcc agaaacaagc gattatccat ttaaagaatt accgctttat gaaataggtt
     7741 ggcgcttgtt gccagaattt tggggaaaag gattagcaac tgaaggcgca aaggcaacat
     7801 tgaagttagc agaagaacat caaatatacg atgtctatag ttttacagca gaagcaaata
     7861 aagcttcaca acgtgtaatg gaaaaaattg gcatgacagt gtatgatcat ttcgaattac
     7921 ccaatctaag taagtatcat ttattaaaaa ggcaagtgcg ctattacatt aatcttcgaa
     7981 agtgaaaaat ttatacataa gcgtaacaaa cacccctaac attgtttagc tgatgataac
     8041 aatattaggg gtgtttgttt attttttaac cttagaatga ttaatcgtat gaacgagtac
     8101 ccagaggttt gaaatttaat attgattcaa ttaatgattc cttagtgtcg cataacggtg
     8161 caagagcacg atacttagga tcaataaaac cttcctcaat catatggtca atcattgttt
     8221 gtagtggatt gaaaaagcca ttaatattat aaatggcaat aggcttttca tggataccta
     8281 tttgagccca actatacatt tcgaaaaatt cttctagtga acctgcgcca ccaggagcca
     8341 tgacaaatgc atctgcaagt tctgccattt tatttttacg ttcatgcata gaatcaacta
     8401 aaattaattc agttaaacgt tggcttgtga tttcatgttc atctaacatt ttaggcatga
     8461 cgccaatagc tttgccgcca tgatctaata caccatcttg aatggcaccc ataatgccaa
     8521 ttgaccctgc accaaatact aattcataac cttgttcagc aaaatattta cctaaatcgt
     8581 atgctttttg tacatatgaa gggtcatgac ctttgcttgc accacaataa actgcgattc
     8641 gtttcatgtt aatccagctc cttaattcga tgaatgactt ttaatagtga ttgttcaaac
     8701 actttttgat cttgctttgt aaaaggtggg ggacctttgt ggcgaccacc ttgttttcta
     8761 atttgtgcat tcatatatcg tttatctaat agttgttgaa tatttttgga attgtatatc
     8821 ttcccattat gatgcatgac aattaagact ttgtcgacta ataaacttgc gagtccataa
     8881 tcttgagtga ctacgatatc atccttcgtt gataattgaa caattttgta atcaactgca
     8941 tctggtccat catcaacata taatgttgat acatgtggag gatataattg gttcgaaaaa
     9001 tggctgaagc tccgaataat tgtcacaaaa atgcctgtct cagttgttaa atctataata
     9061 gaatcaacaa caggacaagc atctccatca ataataatat gtgtcacaat tatgcctctg
     9121 tattgttttc tttattttgt tgagaggcgc ttttggcaac ataatcttta tattttttaa
     9181 atgacttgat gcgtgcttta tcagcttctt gttggcgttt ttgttcttct ttgtgtcgtt
     9241 tttcaatatt tttttgtaac tttttattca ttttagcgat ttctttgcga tttttttcag
     9301 ctagtttatc gccttttttc tcagttttct catctaattt attaggtgtt aagcctgctt
     9361 tttcttcgta tttttgtgat tttttcatat ctttaatacg ttgtatttca ttcttttcgc
     9421 gggctttttg ctcttcttta tgacgctttt cgatattttt ttgaagtatt ttattcattt
     9481 tatcagcgtc tttacgattt tgtttagcta atttttcgcc ttttttctca atataggcag
     9541 gatcatgttc tctagcaaac tttttaagtt cacgtttatt ttcaaaatct tgttttttat
     9601 cgccgacata ttctttaaca tcactcgctg tgttactgat tgctgcagat gtttttgaag
     9661 caactttact tgtagcatct gtaacttttt gtacgtccgg atgttgtttg atacgtttac
     9721 gttcaacaat taacggtacc aatacaattg gtaatacatt aatcataaat ttgatgactt
     9781 ttttcttatc catagatctt gcctccataa ttactttatt aattttacat accctatgat
     9841 acatcaatat aaacgatgat agtagtgaat cactattaag tatttcagat gttttttaaa
     9901 agaagacaat aaaaactgcc aatcaagtga ttccttaatt gacagtctat attttaaaag
     9961 gaaattaaat acctttacca atgccaaatc cgaagtaaag tatagcaata aagattacta
    10021 atacaattct gtaaatggca aatggaatta gtttgatttt gttaattaga tgcaagaatg
    10081 ttttgattgc aattagtcca acagtaaatg cagctaaaaa gcctaaaata taaaaaggta
    10141 tatcagcaat ctgaatatct tgataatgtt ttaataaaga taaaccacta gctgctaaca
    10201 taattggaac agccataata aatgtaaagt ccgatgctgc tttatgattt aatttcatta
    10261 ataccccagt tgaaattgtt gagcctgaac ggctgaaacc aggccacata gctactgctt
    10321 gagaaatacc aattacaaat gcttggaaat aactgatttg atctactgtt tgtgggtttt
    10381 taactttagc tgagtattta tcagcaataa tcatatagat agcacctacg aataagccaa
    10441 tcataacagt tggcacacta aataaatgtt cttcgatgaa atcatcaaat agtaagccta
    10501 aaatacctgc tggcaccata cccactaata catgtaataa atttaaacgt cttggctttg
    10561 aacgtctttg ttgatcgtta tctccttcaa catgtttgtg tttaccaata tgtaaaatct
    10621 ctaagaagcg ttcgcggaac acccatgctg ctgcaaagac ggatcctaat tggatgacga
    10681 ttttaaatgt aaatgctgac tgagaaccta aaaattcaga tgattttaac cacatatcat
    10741 caactaggat catatgtcca gtagaggaaa caggtgcaaa ttctgttaat ccttcgacga
    10801 cccctaagat aatacctttt attaattcaa tgataaacat aatgtaccca ctttcattac
    10861 tcaatttaat ttatttaaat atcaaaatta ccatatcatg atagcatatt catttaaaga
    10921 catgctagtt atagttataa tactagacta aagatgtata tattca
//
LOCUS       DAXWFK010000086        10946 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.086, whole genome shotgun sequence.
ACCESSION   DAXWFK010000086 DAXWFK010000000
VERSION     DAXWFK010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10946
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.086"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(340..428)
                     /locus_tag="ACGEXS_001670"
     tRNA            complement(340..428)
                     /locus_tag="ACGEXS_001670"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(391..393),aa:Ser,seq:gga)
     gene            complement(483..554)
                     /locus_tag="ACGEXS_001671"
     tRNA            complement(483..554)
                     /locus_tag="ACGEXS_001671"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(519..521),aa:Glu,seq:ttc)
     gene            complement(556..630)
                     /locus_tag="ACGEXS_001672"
     tRNA            complement(556..630)
                     /locus_tag="ACGEXS_001672"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(596..598),aa:Asn,seq:gtt)
     gene            complement(645..718)
                     /locus_tag="ACGEXS_001673"
     tRNA            complement(645..718)
                     /locus_tag="ACGEXS_001673"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(684..686),aa:Gly,seq:tcc)
     gene            complement(734..809)
                     /locus_tag="ACGEXS_001674"
     tRNA            complement(734..809)
                     /locus_tag="ACGEXS_001674"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(774..776),aa:His,seq:gtg)
     gene            complement(823..895)
                     /locus_tag="ACGEXS_001675"
     tRNA            complement(823..895)
                     /locus_tag="ACGEXS_001675"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(860..862),aa:Phe,seq:gaa)
     gene            complement(912..987)
                     /locus_tag="ACGEXS_001676"
     tRNA            complement(912..987)
                     /locus_tag="ACGEXS_001676"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(952..954),aa:Asp,seq:gtc)
     gene            complement(1009..1082)
                     /locus_tag="ACGEXS_001677"
     tRNA            complement(1009..1082)
                     /locus_tag="ACGEXS_001677"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1046..1048),aa:Met,seq:cat)
     gene            complement(1554..2108)
                     /locus_tag="ACGEXS_001678"
     CDS             complement(1554..2108)
                     /locus_tag="ACGEXS_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RBBP9/YdeN family alpha/beta hydrolase"
                     /protein_id="HFF8012679.1"
                     /translation="MTDVIIVHSMHGNSRNHWYQWLEHNLTLEGYDVTLFNFESPEAK
                     TVDQWIEAMTKQINVRKKDTYFVTHGLGSITALKYIEMIDQPIEGFFSIAGFKEDAEN
                     IDLDIDLSNVTIDYDNIKKKVDNFYGLSSKNDKYVSYKETQRLMNALEGNLRIVEDGG
                     HFLEEDGFETFTALQDRMQDYMTR"
     gene            complement(2431..3831)
                     /gene="hemY"
                     /locus_tag="ACGEXS_001679"
     CDS             complement(2431..3831)
                     /gene="hemY"
                     /locus_tag="ACGEXS_001679"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HFF8012680.1"
                     /translation="MTKSVAIIGAGITGLSSAYFLKQQDPNIDVTIFEASNRPGGKIQ
                     SYRKDGYMIELGPESYLGRKTIMTELAKDIGLEQDIVTNTTGQSYIFAKNKLYPIPGG
                     SIMGIPTDIKPFVTTKLISPLGKLRAGFDLLKKPTQMQDGDISVGAFFRARLGNEVLE
                     NLIEPLMGGIYGTDIDKLSLMSTFPNFKEKEEAFGSLIKGMKDEKNKRLKQRQLYPGA
                     PKGQFKQFKHGLSSFIEALEQDVKNKGVTIRYNTSVDDIITSQKQYKIVYNDQLEEVY
                     DGVLVTTPHQVFLNWFGQDPAFDYFKTMDSTTVATVVLAFDEKDIENTHDGTGFVIAR
                     TSDTDITACTWTSKKWPFTTPEGKVLIRAYVGKPGDTVVDDHTDNELVSIVRRDLSQM
                     MTFKGDPEFTIVNRLPKSMPQYHVGHIQQIRQIQAHIKQTYPRLRVTGASFEAVGLPD
                     CITQGKVAAEEVIAEL"
     gene            complement(3855..4778)
                     /gene="hemH"
                     /locus_tag="ACGEXS_001680"
     CDS             complement(3855..4778)
                     /gene="hemH"
                     /locus_tag="ACGEXS_001680"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFF8012681.1"
                     /translation="MTKKMGLLVMAYGTPYKESDIEPYYTDIRHGKRPSEEELQDLKD
                     RYEFIGGLSPLAGTTDDQADALVSALNKAYADVEFKLYLGLKHISPFIEDAVEQMHND
                     GITEAITVVLAPHYSSFSVGSYDKRADEEAAKYGIQLTHVKHYYEQPKFIEYWTNKVN
                     ETLAQIPEEEHKDTVLVVSAHSLPKGLIEKNNDPYPQELEHTALLIKEQSNIEHIAIG
                     WQSEGNTGTPWLGPDVQDLTRDLYEKHQYKNFIYTPVGFVCEHLEVLYDNDYECKVVC
                     DDIGANYYRPKMPNTHPLFIGAIVDEIKSIF"
     gene            complement(4836..5873)
                     /gene="hemE"
                     /locus_tag="ACGEXS_001681"
     CDS             complement(4836..5873)
                     /gene="hemE"
                     /locus_tag="ACGEXS_001681"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFF8012682.1"
                     /translation="MVHNKNNTILKMIKGEETSHTPVWFMRQAGRSQPEYRKLKEKYS
                     LFDITHQPELCAYVTHLPVDNYHTDAAILYKDIMTPLKPIGVDVEIKSGIGPVIHNPI
                     KTIQDVEKLSQIDPERDVPYVLDTIKLLTEEKLNVPLIGFTGAPFTLASYMIEGGPSK
                     NYNFTKAMMYRDEATWFALMNHLVDVSVKYVTAQVEAGAELIQIFDSWVGALNVEDYR
                     RYIKPHMIRLISEVKEKHDVPVILFGVGASHLINEWNDLPIDVLGLDWRTSINQAQQL
                     GVTKTLQGNLDPSILLAPWNVIEERLKPILDQGMENGKHIFNLGHGVFPEVQPETLRK
                     VSEFVHTYTQR"
     gene            complement(5863..5973)
                     /locus_tag="ACGEXS_001682"
     CDS             complement(5863..5973)
                     /locus_tag="ACGEXS_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012683.1"
                     /translation="MIKCQFYDIYLLKIRTNERYDNIDKNDFNLGGLYGA"
     gene            6136..6639
                     /gene="traP"
                     /locus_tag="ACGEXS_001683"
     CDS             6136..6639
                     /gene="traP"
                     /locus_tag="ACGEXS_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein TRAP"
                     /protein_id="HFF8012684.1"
                     /translation="MKKLYTSYGTYGFLHQIKINNPTHQLFQFSASDTSVIFEETDGE
                     TVLKSPSIYEVIKEIGEFSEHHFYCAIFIPSTEDHAYQLEKKLISVDDNFRNFGGFKS
                     YRLLRPAKGTTYKIYFGFADRHAYEDFKQSDAFNDHFSKDALSHYFGSSGQHSSYFER
                     YLYPIKE"
     gene            complement(6763..7986)
                     /locus_tag="ACGEXS_001684"
     CDS             complement(6763..7986)
                     /locus_tag="ACGEXS_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8012685.1"
                     /translation="MRNQATTLFNKRLHALRKEKNYYNKFIFNGHFMVFLLILLGAFI
                     FGYGEWLKHIPTNINFSLIAAVIVALTSIFPMRPLLKEADKIFLLPFEKHMSQFMRHA
                     ILYSYFARILIQLIIVIVMFPLFYNINQHNVAFYICFGVSALIFPYVGLRLRWQWYQS
                     GLKTWQVNLISFITFALTYYLLLAPKWYIAFVMVALPVLIEFLVKKYKPGFLYPWEKM
                     IAIEHRHHMNYYKFVNMFTDVKHLKESAVRRSYLDILLPVPKGSKFNSNAMYLFLFIR
                     SFIRGRDAFNIIFRLVIIAVLLMVWLSYPLVTAIIGCLFVYIILLQMAQFYSQQAYGL
                     WPQVWPVPEEKVIKGYEQFLYRLMFVICTVFAVTFIIKHMTLFYVVLIFYIVGLLTIR
                     SIIKKLKYQETLLRD"
     gene            complement(7979..8719)
                     /locus_tag="ACGEXS_001685"
     CDS             complement(7979..8719)
                     /locus_tag="ACGEXS_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8012686.1"
                     /translation="MTVKVEQLTGGYGKRPVIKDINFELNKGEIVGLIGLNGAGKSTT
                     IKHMLGLLTPMEGSLSISDININDDIEAYRRKLSYIPESPVIYEELTLEEHIEMTAMA
                     YDIDRDETMNRAMPLLKTFRLENELKVFPSHFSKGMKQKVMIICAFIVNPELYIIDEP
                     FLGLDPLGIQSMLDLMVEKKNEGRTVLMSTHILATAERYCDRFIILDEGEVVAFGDLE
                     ALRQQTGLHNQTLDDIYIHVTQGGDVHA"
     gene            8853..9275
                     /locus_tag="ACGEXS_001686"
     CDS             8853..9275
                     /locus_tag="ACGEXS_001686"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFF8012687.1"
                     /translation="MSETIFGKILTGEIPSFKVYEDDYVYAFLDISQVTKGHTLLIPK
                     KASANIFETDEETMKHIGAALPKVANAIKRAFNPDGLNIIQNNGEFADQSVFHIHFHL
                     IPRYENDIDGFGYKWETHEDILDNDAKQQIAEQIQAQF"
     gene            9417..9782
                     /locus_tag="ACGEXS_001687"
     CDS             9417..9782
                     /locus_tag="ACGEXS_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFF8012688.1"
                     /translation="MKASRILFGIGVGVAAGFVVALQGRDDKSVKNNTIDRTAPTGSK
                     SELQREFETIKQSFNDILNYGVQIKNESSEFGSSIGGEIKSLLGNFKSDINPNIERLQ
                     SHIENLQNRGEDIGNEISK"
     gene            10515..>10946
                     /locus_tag="ACGEXS_001688"
     CDS             10515..>10946
                     /locus_tag="ACGEXS_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HFF8012689.1"
                     /translation="MFLCKRQIDINARFGLPRIAFMSAVATIIMFLVSYEVMYFLSNT
                     PLSDRHFLIFLLLVFMTYPLHKSIHLLFFLPYRKSFKVHKLTKRKWLIFYNTYVNQPV
                     HKFYFCINLILPLIILSAMFVYLTISFPQYGHYFMFLLALNF"
BASE COUNT         3592 a         2028 c         1593 g         3733 t
ORIGIN      
        1 acagctaaac attacatttc aaaacgatta ggtcatgcag atgtacatac aacgcttaaa
       61 acatattcac atttgattaa agaatttgaa gataatgaaa ctaaatttat agaaaaaaat
      121 ctgaatgatt tgttttgtga ctaaacttca aaatcttgtg actaaaatta ggattattca
      181 tcaaaaatta gtcacattgt ggctaaaaaa ttttaaatgg atataaataa ataagaagtg
      241 taaaaatgag gataatagct caaatgcaga tatatcaagg gattgagtcc aaaataaaaa
      301 tagcgcttaa aatgggtaaa accaatttaa gcgcctttac ggaaggtaag ggattcgaac
      361 ccttgagacg cttgtgacgc ctacacactt tccaggcgtg ctccttcggc cagctcggac
      421 aaccttcctc aacaataaat atgtaataaa aaaacagaag cgatatttca cttctgcttg
      481 tatgactcct acgggactcg aacccgtgtt accgccgtga aagggcggtg tcttaaccgc
      541 ttgaccaagg agccatggct ccacaggtag gactcgaacc tacgaccgat cggttaacag
      601 ccgatagctc taccactgag ctactgtgga ataatagaaa ataatggagc gggtgatggg
      661 aatcgaaccc acaacatcag cttggaaggc tgaggttttg ccattaaact acacccgcta
      721 atgtaaacga ttatggggcg gctgaagggg atcgaaccct cgaatgtcgg aaccacaatc
      781 cgatgtgtta accacttcac cacaaccgcc atgaattaag aatggttcag gacagagtcg
      841 aactgccgac acatggagct tcaatccatt gctctaccaa ctgagctact gaaccgtaat
      901 ggtaataata atggcggtct cgacgggaat cgaacccgcg atctcctgcg tgacaggcag
      961 gcgtgttaac cgctacacta cgagacctat taaattaaaa actatgtatt gcgggaggcg
     1021 gatttgaacc accgaccttc gggttatgag cccgacgagc taccgaactg ctccatcccg
     1081 cgctaatatt attttgaatt acctaattaa tataccataa tcaaaaacct aaagtcaaga
     1141 actttttgaa tttaatttaa atgttatctc ttaaataatt acttaaatat cgtagcaaca
     1201 tgttctctgt tgaacacaaa tattagtata ttcatttttg tagtgtacgt caacgacatt
     1261 ttcaaagttt tttgtgtaaa aaaacgcttc ttattccctt ttatcatata agtgtctaat
     1321 agttgtcata aatagtgtta aagcatttaa aaaggtatag gagttataaa gtttacaacg
     1381 cctatacctt ctgaaaaaga aattataaaa gcttgttaca ccgcatattc tttcagtcag
     1441 cgactaccaa tataacattg tagccctaag acattgcttg acgcctcaat tacaacaatt
     1501 tttcaaaatc agcagctacc tactgacaca acataacaca acccatacac taactatcgt
     1561 gtcatgtaat cttgcatccg atcttgcaac gctgtaaatg tttcgaagcc atcctcttct
     1621 aagaagtgcc ctccatcttc cacgattcgc aagttcccct ctaatgcatt cattaaacgc
     1681 tgggtctctt tatatgaaac gtatttgtca tttttagaac tcaatccgta aaaattgtca
     1741 actttctttt taatattatc gtaatcaatg gttacattac ttaaatcaat atctaaatct
     1801 atattttctg catcttcttt aaagcccgct atactgaaaa agccttcaat cggctgatca
     1861 atcatttcaa tatattttaa agctgtgatt gaacctaaac catgtgttac aaaatatgta
     1921 tcctttttgc gtacattaat ttgtttcgtc atagcttcaa tccactgatc cactgtcttc
     1981 gcttcagggg attcaaaatt aaataatgtt acgtcatatc cttctaaagt taagttatgc
     2041 tccaaccact gataccaatg atttctacta tttccatgca tagaatgtac aataattaca
     2101 tctgtcatct cattctctcc tttcaactta ctacttcttt tctattttta aaaaaatgac
     2161 tgattaccta taattgtaaa ataaaaacac cttaattaga aatgttatat cgcaaagtga
     2221 catttctaat taaagtgtat tgtcatcatt tcaatatcat tcaaaaacag ctaaaccttt
     2281 gtctctgttt caatttcaca aaaataattc ccgctgaaag tatctatatt tacacattac
     2341 ttccaccatt atataactta aaaatgacta tatttcatca aacattatct aaaggcgtcg
     2401 cacctacacc aacaccatcc aacaattaac ttacaactct gcgattactt cttcagcagc
     2461 aactttacct tgcgtaatac aatcaggtag tccaaccgct tcaaaagatg caccagttac
     2521 tctaagtcgt ggatatgttt gtttaatatg tgcttgaatc tgtctaattt gttgaatatg
     2581 accgacatgg tactgtggca tacttttcgg caaacgattg acaattgtaa attcaggatc
     2641 acctttaaat gtcatcattt gacttaaatc tctacgtaca atcgatacta attcattatc
     2701 tgtatgatca tcaaccacag tatcacctgg tttacctaca tacgcacgaa tcaaaacctt
     2761 accttccggt gtagtaaatg gccatttttt cgatgtccaa gtacatgcgg taatgtctgt
     2821 atcactcgtt ctcgcaatta cgaagccagt accatcatgg gtattttcaa tgtctttttc
     2881 atcaaatgcc aatacaacag ttgcaacagt cgtactatcc atcgttttaa agtaatcaaa
     2941 tgctggatct tgtccgaacc aatttaaaaa cacttgatgt ggtgtcgtta ctaatacgcc
     3001 atcatacact tcttctagtt gatcattgta aacaatttta tattgttttt gagatgtaat
     3061 tatatcatcc actgacgtat tgtagcgtat tgtcacacct ttatttttaa catcttgttc
     3121 taatgcttca ataaatgagc ttaaaccatg cttaaattgt ttgaattgtc ctttcggtgc
     3181 gccaggatat aattgtcttt gtttcagacg cttatttttc tcatccttca taccttttat
     3241 cagacttccg aatgcctctt ctttttcttt aaaattagga aacgtactca tcaaacttaa
     3301 tttatcaata tcggtaccat aaataccacc cattaaaggc tctattaaat tctcaagtac
     3361 ctcattacct aatcttgctc tgaaaaatgc accaacagaa atgtcaccat cctgcatttg
     3421 agtaggtttt tttaataaat caaaccctgc tcttaattta ccaagtggcg atattaattt
     3481 tgtagtaaca aatggtttaa tatctgttgg aatacccata attgaaccac ctggaatcgg
     3541 atataattta tttttcgcaa aaatatatga ttgtccagtc gtatttgtaa caatatcttg
     3601 ttctaatcca atatctttcg ctaattctgt cataatcgtt tttctaccta aataagattc
     3661 aggccctagt tcaatcatat aaccatcttt acgatacgat tgaatctttc cccccggacg
     3721 attcgatgct tcaaagatgg ttacatcaat attaggatct tgctgtttta aaaaatatgc
     3781 acttgataaa cctgttatcc ccgctcctat aatagccact gatttagtca caacgtttca
     3841 cgcttctttc gtcattaaaa tatagacttg atttcatcaa caattgcacc gataaataat
     3901 ggatgtgtat tcggcatttt tggacgataa taattcgcac caatatcatc gcaaactact
     3961 ttacattcat aatcattgtc ataaagcacc tctaaatgct cacatacaaa acctactggc
     4021 gtatatataa agtttttata ctgatgtttt tcatataaat cacgtgttaa atcttgtaca
     4081 tctggcccta accaaggtgt acctgtatta ccttcagatt gccaaccaat cgcgatatgt
     4141 tcaatattag attgttcttt aattaaaagc gcagtatgtt ctagttcttg tggatatgga
     4201 tcattattct tttcgattaa accttttggc aaactatgtg ccgaaacaac taataccgtg
     4261 tctttatgtt cctcttccgg tatttgagct aatgtttcgt tgactttatt cgtccaatat
     4321 tcaataaatt taggttgttc ataataatgt ttcacatgtg taagttgaat accatatttt
     4381 gcagcttctt catcagcacg tttgtcatat gatcctactg aaaatgaaga ataatgtggt
     4441 gctagtacta ccgtgattgc ttcagtaatg ccatcattgt gcatttgttc aaccgcatct
     4501 tcgataaatg gtgaaatgtg ttttaatcct aagtatagtt taaattcaac atctgcatat
     4561 gctttattta atgctgaaac tagcgcatca gcctggtcat ctgttgtacc tgctaatggt
     4621 gataaaccac ctataaattc atatctatct ttcaaatctt gaagttcttc ttcagatgga
     4681 cgtttaccat gtctaatatc tgtataatat ggctctatgt cactttcttt ataaggtgtg
     4741 ccataagcca taactaataa tcccattttt ttagtcattg ataatacctt cctttaaatg
     4801 aattatcttt catgtgcttc aatgtaaaac tatgattatc tttgtgtata tgtgtgtacg
     4861 aattcgctta ctttacgtaa cgtctctggt tgcacttctg ggaatacacc gtgtcctaaa
     4921 ttaaagatgt gtttaccgtt ctccatacct tgatctaata ttggtttcaa tctctcttca
     4981 atgacattcc atggtgctaa taaaattgat ggatctaaat tcccttgtaa tgttttagta
     5041 acgcctaatt gttgagcctg attaatagac gttctccaat ctaggcctaa tacatcaatc
     5101 ggtaaatcat tccattcatt gattaaatga ctggcaccta caccgaataa aattaccggc
     5161 acatcatgtt tttctttaac ctcactgatt aatcgaatca tatgtggttt aatgtaacgt
     5221 ctgtaatcct cgacatttaa tgcacctacc catgaatcga aaatttgaat caattcggca
     5281 cctgcttcga cttgagctgt tacatattta acagatacat caactaaatg attcattaaa
     5341 gcaaaccatg ttgcttcatc tctatacatc atcgcttttg taaaattgta atttttcgat
     5401 ggtccgcctt caatcatata tgacgctaat gtaaatggtg ccccagtaaa tcctattagc
     5461 ggcacattta acttttcttc tgttaaaagt ttaattgtat ctaatacata tggtacatct
     5521 cgttcggggt ctatttgaga aagtttctca acatcttgaa ttgttttgat tggattatga
     5581 atcactggac caatacccga tttaatttct acatcgacac caattggctt taatggtgtc
     5641 ataatatctt tgtataaaat tgctgcatct gtatgataat tatcaactgg taaatgtgtt
     5701 acataagcgc acaactccgg ctgatgtgta atatcgaata gtgaatattt ttctttcaat
     5761 tttcgatatt ctggttgcga acggccagct tgtcgcataa accaaacagg tgtatgtgat
     5821 gtttcttcac ctttgatcat ttttaaaatt gtattgtttt tattatgcac cataaaggcc
     5881 tcctaaatta aaatcattct tatctatatt atcatatcgc tcattcgttc gtattttcaa
     5941 taaataaatg tcataaaact gacatttaat catagaacta tttattgtaa atttaaattc
     6001 taaagtccat tattttgtat cattacttct aaatatctcg caagattcat tatagtaatt
     6061 ttaatcaatt attaatagtg gtaatgacta gtttatcatc gtataataaa taaaaacata
     6121 agggggacct ttcatatgaa gaaactatat acatcttatg gcacttatgg atttttacat
     6181 cagataaaaa tcaataaccc gacccatcaa ctattccaat tttcagcatc agatacttca
     6241 gttatttttg aagaaactga tggtgagact gttttaaaat caccttcaat atatgaagtt
     6301 attaaagaaa ttggtgaatt cagtgaacat catttctatt gtgcaatctt cattccatca
     6361 acagaagatc atgcatatca acttgaaaag aaactgatta gtgtagacga taatttcaga
     6421 aactttggtg gctttaaaag ctatcgtttg ttaagacctg ctaaaggtac aacatacaaa
     6481 atttatttcg gatttgctga tcgacatgca tacgaagact ttaagcaatc tgatgccttt
     6541 aatgaccatt tttcaaaaga cgcattaagt cattactttg gttcaagcgg acaacattca
     6601 agttattttg aaagatatct atacccaata aaagaataga ttcgaagaaa acttccattg
     6661 gttatgctgt taacgtatag atgctaatgt aatctgaacg ccgtatcatt tggcgttcaa
     6721 cattacatgc acctttacaa catacatgcc aatggaagtt ttttagtctc gtaataatgt
     6781 ttcctgatat ttcaactttt taataatact tctaatcgtt aataaaccta caatatagaa
     6841 aatgagtaca acatagaata acgtcatatg ttttataata aatgtcactg caaacacagt
     6901 acaaataaca aacattaatc tatataaaaa ctgttcatac cctttgatta ccttctcttc
     6961 aggtacaggc cacacttgag gccataagcc atatgcttgc tgtgaataaa attgtgccat
     7021 ttgtaacaat ataatatata caaataaaca cccaataatt gctgtcacta atggatatga
     7081 taaccaaacc attaataaaa ctgcaataat tactaaccta aagataatat taaatgcgtc
     7141 tctccctctt ataaagcttc taataaataa gaataaatac atcgcattag agttaaattt
     7201 actacccttt ggaactggta aaagtatatc tagataactt cttctgactg cagattcttt
     7261 caaatgtttt acatcggtga acatattaac aaatttataa taattcatat gatgtcgatg
     7321 ttcgattgca atcattttct cccaaggata caaaaagcct ggtttatatt ttttaactaa
     7381 aaattctatt aacacaggca aagcaaccat cacaaatgcg atgtaccatt ttggagctaa
     7441 tagtaagtaa tatgttagag caaaggtgat gaatgatatt aaattaactt gccatgtttt
     7501 aagtcccgat tgataccatt gccatcttaa gcgtaaacca acatatggaa aaattaatgc
     7561 actgactcca aaacaaatat aaaatgccac attatgttga ttaatattgt aaaacaacgg
     7621 gaacattaca ataacaataa tgagttggat taatatgcgc gcaaagtaac tatataaaat
     7681 cgcatgacgc ataaattgag acatgtgttt ttcaaatggt aataaaaaga ttttatccgc
     7741 ttcttttaac agtggtcgca ttggaaaaat agatgtcaac gcaacaatca ctgctgctat
     7801 taatgaaaaa ttgatattcg ttggaatatg ttttaaccat tcaccatatc caaaaataaa
     7861 tgcacccagc aaaataagta aaaagaccat gaaatgacca ttaaatataa acttattata
     7921 ataatttttc tctttacgaa gggcatgtaa tcttttatta aataatgtgg tagcttggtt
     7981 acgcatgtac atctccacct tgcgtcacat gaatatatat atcgtctaat gtttgattat
     8041 gtaagccagt ttgttgtctc aatgcttcta aatctccaaa tgcaacgact tcaccttcgt
     8101 ctagtatgat aaaacgatca cagtaacgtt cagctgttgc taaaatatgt gtactcatta
     8161 gaacggttct accttcgttt ttcttttcaa ccattaaatc taacatggat tgaattccta
     8221 atggatctag gccaaggaat ggttcgtcta taatatacaa ttcgggatta acgataaacg
     8281 cacaaataat catgactttt tgtttcatcc ccttagaaaa atgactcgga aaaactttca
     8341 actcattttc taaacggaat gtctttaata atggcattgc tcgattcatc gtttcatcac
     8401 gatcaatatc atatgccatt gctgtcatct caatgtgttc ctctaatgtg agttcttcat
     8461 aaataaccgg tgattccgga atataagata actttcttct ataagcctct atgtcatcat
     8521 taatgttgat atctgaaatt gatagagatc cttccatagg tgtaagcaat cctagcatat
     8581 gtttaatcgt tgtactctta ccagcgccat taaggccaat aagtccaaca atttcgcctt
     8641 tgtttaattc aaaatttata tctttaatta cagggcgttt tccatatcca cctgtaagct
     8701 gttctacttt aactgtcata aggcacctcc atgacttata ttgtaccaaa aattataaaa
     8761 tgctcatatt aaatacacat gtcctaatat cgaattttta gcgacaatgt tataatgaat
     8821 ggtaatacta gttgaaaagg agtgtagtca tcatgtcaga aacaattttc ggcaaaattt
     8881 taactggaga aattcctagc tttaaagtat atgaagacga ttatgtctat gcctttttag
     8941 atatatcaca agttactaaa ggacatacgt tattaattcc taaaaaagct tctgctaata
     9001 tctttgaaac tgatgaagaa acaatgaaac atatcggtgc agcattacct aaagtagcaa
     9061 atgctattaa gcgtgcattt aatcctgatg gtttaaacat tattcaaaat aatggtgagt
     9121 ttgcagatca atctgtattt catattcatt tccacttaat tcctcgatac gaaaatgata
     9181 ttgatggatt tggttataag tgggaaacac atgaagacat tttagataac gatgcaaaac
     9241 aacaaattgc tgaacaaatt caagcacaat tttaaatgta tgcttaatct aagctcgaac
     9301 gggtataata tgattaatat tataacaatt gcgtttgaag tgataacatc aaggttagca
     9361 attttaaaca aaatgagtta tcaagataac agatgttaaa agtgaggaga atataaatga
     9421 aagcatcacg cattctattc ggtatcggtg ttggcgtagc agctggtttt gtagttgcac
     9481 ttcaaggacg tgacgacaaa agtgtcaaga acaacacgat cgatcgtact gcccctactg
     9541 gttcaaaatc agaactacaa cgtgaatttg aaacgattaa acaaagtttt aatgacattt
     9601 taaactatgg tgttcaaatt aaaaacgaaa gttcggaatt tggtagttca attggtggtg
     9661 aaattaagtc attacttgga aacttcaaat ctgacattaa tcctaatatt gaacgtttac
     9721 agtcacacat cgaaaattta caaaatcgtg gcgaggatat tggaaacgaa atttctaagt
     9781 agcaggttac gttctcgatc acaactattt ttattagtaa cagcatattt attttttaaa
     9841 attaaatgcc aaataaacga gatgacatta gaaattagat atttcttgtc atctcttttt
     9901 taaaactcaa atgaacttat gtttacaaat tataggaaga cattgtttgt agtgattttc
     9961 gcttaaatca tatttatgaa ttgattgaaa acattgctta ggattcattg tgttatcctt
    10021 gcactttgat tacgctttac ttaaatcatt atcgacaaac aacatactta tattttcatt
    10081 gagccgaacc ttatatacac attacatata ccttacttgc acaaattatt aatctggtgt
    10141 ttattataat tacatatcac tatattttta gcatttgtat aacttagttg gtcaaaagat
    10201 gcttttgcat atgccctgcc atatccattt tttatattat tcaagttttc aaatactcat
    10261 gtcacacgcg cctttttcat aagtgatgct ttattagcaa gaatatgtgt tcgcagaaat
    10321 ttgttctgca ttctacttct acgctagtca atcagacaat tttaccaatc cccactttcg
    10381 cgtttcaaat caaacaatac gtcgctcctt tcttcttata taacaattct tctaacatga
    10441 tatgttacta ttgaattact gaacctgagt tagttataat ctaacttata ttgaaaagag
    10501 atgaggcgta agatatgttt ttatgtaaaa gacaaattga tatcaatgca cgatttggtt
    10561 tgcctagaat tgcatttatg agtgcagttg caaccatcat tatgttttta gttagttatg
    10621 aagtaatgta ttttttatct aatacgccat tatcagatag acattttctc atctttttat
    10681 tacttgtatt tatgacgtat ccattacata aaagtataca tttattattt ttcttaccat
    10741 atagaaaatc gtttaaagtt cataagttaa ctaaaagaaa atggcttata ttctataata
    10801 cctacgtcaa tcaacctgta cacaaatttt atttttgcat taacttaata ttgccgttaa
    10861 ttatcttatc tgcaatgttc gtttatctaa caatttcatt cccgcaatat ggacattatt
    10921 ttatgttctt attggcattg aatttc
//
LOCUS       DAXWFK010000087        10929 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.087, whole genome shotgun sequence.
ACCESSION   DAXWFK010000087 DAXWFK010000000
VERSION     DAXWFK010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10929
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.087"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            158..1000
                     /locus_tag="ACGEXS_001689"
     CDS             158..1000
                     /locus_tag="ACGEXS_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFF8012690.1"
                     /translation="MKIGDISIHYLNGGNTKMDGGAMFGVVPKPLWSKQYNANERNQI
                     NLPTHPILIQTAQYNLIIDAGIGNGKLSEKQLRNFGVDEESHIIADLANYNLTPKDID
                     YVLMTHMHFDHAAGLTDQAGHAIFENAIHVVQQDEWHEFIAPNIRSKSTYWDKNKGDY
                     SNKLILFEKHFEPVLGIKMQHSGGHSFGHTIITIESQGDKAVHMGDIFPTTAHKNPLW
                     VTAYDDYPMQSIREKERMIPYFIQQQYWFLFYHDENYFAVKYSDNGENIDAYILRETL
                     VDNN"
     gene            complement(1125..1436)
                     /locus_tag="ACGEXS_001690"
     CDS             complement(1125..1436)
                     /locus_tag="ACGEXS_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFF8012691.1"
                     /translation="MTKLKYIIPTIIAVAIVIISTISIIQFINRKRYNPVKVLNEVKS
                     YFMNVKGSYIVYEPFVHPETDKYRLVYQGGITTIKNGQNIHYDFYADAYTGEVINIVE
                     R"
     gene            1523..2599
                     /locus_tag="ACGEXS_001691"
     CDS             1523..2599
                     /locus_tag="ACGEXS_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFF8012692.1"
                     /translation="MNINKKVTLQRIQTLTELHGAPGFEEEVKNYMTQQMAPYVDEFI
                     ENRMGGFFGVKKSKNPNAKRVMIAAHMDEIGFMITNITKNGMIQFTNLGGVANDIWQG
                     QRLVIKNRNGDKIIGVVSNIPKHFRTGSEGAPEIKDLTLDIGAQNEDEVRERGIDIGD
                     TIVPHTPFTQLSEHRYSAKAWDNRYGCVLAIEILELLKDIELDVDLYVGANVQEEVGL
                     RGAKASAEMIDPDVAFVVDCSPANDVKGNQPLSGELGKGTLIRIKDGTMILKPVFRDY
                     LLKLVEAHDIEHQYYMSPGGTDGGEIHKANIGIPTAVIGVCARYIHSTDSVFDIRDYF
                     AARSLLSEAICNLDNNQIETLQYK"
     gene            2664..2975
                     /locus_tag="ACGEXS_001692"
     CDS             2664..2975
                     /locus_tag="ACGEXS_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFF8012693.1"
                     /translation="MKQLESEQQFESLKQGATVFEFTAGWCPDCRVIEPDLPELEARY
                     PMFDFVSVDRDKFMDICIENGIMGIPSFLVYKNGELLGSYIGKERKSIEQIDAFLAQY
                     V"
     gene            3075..3932
                     /locus_tag="ACGEXS_001693"
     CDS             3075..3932
                     /locus_tag="ACGEXS_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1444 domain-containing protein"
                     /protein_id="HFF8012694.1"
                     /translation="MNTFQMRDKLKERLSHLDVDFKFNREEETLRIYRTDNNKGITIK
                     LNAIVAKYEDKKEKIVDEIVYYVDEAIAQMADKTLESISSSQIMPVIRATSFDKKTKQ
                     GVPFIYDEHTAETAVYYAVDLGKSYRLIDESMLEDLKLTEQQIREMSLFNVRKLSNSY
                     TTDEVKGNIFYFINSNDGYDASRILNTAFLNEIEAQCQGEMLVAVPHQDVLIIADIRN
                     KTGYDVMAHLTMEFFTKGLVPITSLSFGYKQGHLEPIFILGKNNKQKRDPNVIQRLEA
                     NRRKFNKDK"
     gene            3961..4557
                     /gene="ytpR"
                     /locus_tag="ACGEXS_001694"
     CDS             3961..4557
                     /gene="ytpR"
                     /locus_tag="ACGEXS_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFF8012695.1"
                     /translation="MNLFYNPKYVGDVAFLQIEPVEGELNYNKKGNVVEITNEGNVVG
                     YNIFEISKDITIEEKGHIKLTDELVNVFQKRISEAGFDYKLNADLSPKFVVGYVETKD
                     KHPDADKLSVLNVNVGNDTLQIVCGAPNVEAGQKVVVAKVGAVMPSGMVIKDAELRGV
                     ASSGMICSMKELNLPNAPEEKGIMVLNDSYEIGQAFFE"
     gene            4578..8420
                     /locus_tag="ACGEXS_001695"
     CDS             4578..8420
                     /locus_tag="ACGEXS_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFF8012696.1"
                     /translation="MSWFDKLFGEDNDSNDDLIHRKKKRRQESQNIDNDHDSLLPQNN
                     DIYSRPRGKFRFPMSVAYENENVEQSADTISDEKEQYHRDYRKQSHDSRSQKRHRRRR
                     NQTTEEQNYSEQRGNSKISQQSIKYKDHSHYHTNKPGTYVSAINGIEKETHKPKTHNM
                     YSNNTNHRAKDSTPDYHKESFKTSEVPSAIFGTMKPKKLENGRIPVSKPSEKVESDKQ
                     KYDKYVAKTQTSQNKQLEQEKQNDSVVKQGTASKSSDENVSSTTKSMPNYSKVDNTIK
                     IENIYASQIVEEIRRERERKVLQKRRFKKALQQKREEHKNEEQDAIQRAIDEMYAKQA
                     ERYVGDSSLNDDSDLTDNSDLTDNSTDASQLHTNGIENETVSNDENKQASIQNEDTND
                     THVDESPYNYEEVSLNQVSTTKQLSDDEVTVSNVTSQHQSALQHNVEVNDKDELKNQS
                     RLIADSEEDGATNKEEYSGSQIDDAEFYELNDTEVDEDTTSNIEDNTNRNASEMHVDA
                     PKTQEYAVTESQVNNIDKTVDNEIELAPRHKKDDQTNLSVNSLKTNDVNDNHVVEDSS
                     MNEIEKNNAEITENVQNEAAESEQNVEEKTIENVNPKKQTEKVSTLSKRPFNVVMTPS
                     DKKRMMDRKKHSKVNVPELKPVQSKQAVSERMPASQATPSSRSDSQESNTNAYKTNNM
                     TSNNVENNQLIGHAETENDYQNAQQYSEQKPSVDSTQTEIFEESQDDNQLENEQVDQS
                     TSSSVSEVSDITEESEETTHPNNTSGQQDNDDQQKDLQSSFSNKNEDTANENRPRTNQ
                     QDVATNQAVQTSKPMIRKGPNIKLPSVSLLEEPQVIESDEDWITDKKKELNDALFYFN
                     VPAEVQDVTEGPSVTRFELSVEKGVKVSRITALQDDIKMALAAKDIRIEAPIPGTSRV
                     GIEVPNQNPTTVNLRSIIESPSFKNAESKLTVAMGYRINNEPLLMDIAKTPHALIAGA
                     TGSGKSVCINSILMSLLYKNHPEELRLLLIDPKMVELAPYNGLPHLVAPVITDVKAAT
                     QSLKWAVEEMERRYKLFAHYHVRNITAFNKKAPYDERMPKIVIVIDELADLMMMAPQE
                     VEQSIARIAQKARACGIHMLVATQRPSVNVITGLIKANIPTRIAFMVSSSVDSRTILD
                     SGGAERLLGYGDMLYLGSGMNKPIRVQGTFVSDDEIDDVVDFIKQQREPDYLFEEKEL
                     LKKTQTQSQDELFDDVCAFMVNEGHISTSLIQRHFQIGYNRAARIIDQLEQLGYVSSA
                     NGSKPRDVYVTEADLNKE"
     gene            8444..9757
                     /gene="murC"
                     /locus_tag="ACGEXS_001696"
     CDS             8444..9757
                     /gene="murC"
                     /locus_tag="ACGEXS_001696"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFF8012697.1"
                     /translation="MTHYHFVGIKGSGMSSLAQIMHDLGHEVQGSDIENYVFTEVALR
                     NKGIKILPFDANNIKEDMVVIQGNAFASSHEEIVRAHQLKLDVVSYNDFLGQIIDQYT
                     SVAVTGAHGKTSTTGLLSHVMNGDKKTSFLIGDGTGMGLPESDYFAFEACEYRRHFLS
                     YKPDYAIMTNIDFDHPDYFKDINDVFDAFQEMAHNVKKGIIAWGDDEHLRKIEADVPI
                     YYYGFKDSDDIYAQNIQITDKGTAFDVYVDGEFYDHFLSPQYGDHTVLNALAVIAISY
                     LEKLDVTNIKEALETFGGVKRRFNETTIANQVIVDDYAHHPREISATIETARKKYPHK
                     EVVAVFQPHTFSRTQAFLNEFAESLSKADRVFLCEIFGSIRENTGALTIQDLIDKIEG
                     ASLINEDSINVLEQFDNAVILFMGAGDIQKLQNAYLDKLGMKNAF"
     gene            9831..10322
                     /locus_tag="ACGEXS_001697"
     CDS             9831..10322
                     /locus_tag="ACGEXS_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFF8012698.1"
                     /translation="MDWILPIAGIIAAIAFLILCIGIVAVLNSVKKNLDYVAKTLDGV
                     EGQVQGITRETTDLLHKVNRLTEDIQGKVDRLNSVVDAVKGIGDSVQTLNSSVDRVTN
                     SITHNISQNEDKISQVVQWSNVAMEIADKWQNRHYRRGSANYKANNVATDANHSYTSR
                     VDK"
     gene            10396..>10929
                     /locus_tag="ACGEXS_001698"
     CDS             10396..>10929
                     /locus_tag="ACGEXS_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012699.1"
                     /translation="MTQQQNDKRTLKNKHTYQNEPLPNRKDFVVSFITGALVGSALGL
                     YFKNKVYQKADDLKVKEQELSQKFEERKTQLEETVAYTKERVEGFLNKSKNEQAALKA
                     QQAAIKEEASANNLSDTSQEAQEIQEAKREAQAEADKSVAVSNKESKAVALKAQQAAI
                     KEEASANNLSDTSQEAQE"
BASE COUNT         4196 a         1490 c         2053 g         3190 t
ORIGIN      
        1 atgcatttac atataatatg attgcattaa tctttataag tagttagaag tgtattagtg
       61 tggggcgttt cgtttttgtt cgcatttatg aatagtgata tgatgtaata aacacatgct
      121 aattgtaata acgaatacag aaagaatagg tgatgttatg aaaatcgggg atatatctat
      181 tcattatcta aatggtggca atacaaaaat ggatggcggt gcaatgtttg gtgttgttcc
      241 gaagccgttg tggtcaaagc aatacaatgc aaatgaacga aatcaaatca atttaccgac
      301 acatccaatt ttgattcaaa cggctcaata taatttgatt atagatgcag gtattggtaa
      361 tggtaaatta tctgaaaagc aattacgtaa ttttggagta gatgaagaaa gtcatataat
      421 tgctgatttg gcaaattata atttaacgcc aaaggatatt gattatgtgc taatgacaca
      481 tatgcatttt gatcatgctg ccggtttgac tgatcaagca ggacatgcaa tttttgaaaa
      541 tgcgattcat gttgtgcaac aagatgagtg gcatgagttt attgcaccta atataagaag
      601 taaatcaact tactgggata agaataaagg cgattatagt aacaagttga ttttattcga
      661 aaaacatttt gaaccggttc tgggtatcaa gatgcaacat agtggaggtc atagctttgg
      721 ccacacgatt attacgattg aaagtcaagg agataaagca gttcatatgg gtgatatatt
      781 cccaactact gcacataaaa atcctctatg ggtaacggca tatgatgatt atcctatgca
      841 atcgattcgt gaaaaagaac gcatgatacc atattttatt cagcaacaat attggttctt
      901 gttttatcat gatgaaaact actttgctgt aaaatacagc gataatggtg aaaacataga
      961 tgcatatatt ttacgtgaaa cattagttga taataactaa aataaagatg tattactaaa
     1021 caaattttca aaaataaaaa attgagccac atccaatctt actaattagg gtgtggctca
     1081 tttttaagtt ttacgatcca aatcaaatat ggataaaatt cgtattaacg ctctacaatg
     1141 ttaatgactt caccagtata tgcatctgca taaaaatcat aatgaatatt ttgaccattt
     1201 ttaatagttg taattccacc ttgataaact aaacggtatt tatcagtttc aggatgaacg
     1261 aatggttcat aaacgatata tgagcctttg acattcataa aatatgattt tacttcatta
     1321 agtactttaa cgggattata gcgtttacga ttaataaatt ggatgattga aatggtagaa
     1381 ataattacaa tggctactgc aattattgtt ggaattatat atttcagttt agtcatcatt
     1441 tatgctccct tgatttcaaa ttcatatcat tagtttacca tattgaagat gatataataa
     1501 cttataagga gtgagaactt ttatgaacat aaataaaaaa gtaacattac aacgaattca
     1561 aactttaact gagcttcatg gggcaccagg ttttgaagaa gaagtaaaaa attatatgac
     1621 tcagcaaatg gcgccgtacg tagatgaatt tattgaaaat cgtatgggtg gattttttgg
     1681 tgtgaaaaaa tctaaaaatc caaatgcaaa acgtgtaatg attgcagcac atatggatga
     1741 aatcggattt atgattacaa atatcactaa aaatggaatg attcaattca caaatttagg
     1801 tggtgttgca aatgatattt ggcaaggaca acgcttagta attaaaaata gaaatggcga
     1861 taaaattatc ggtgttgttt ctaatatacc taaacatttt cgtactggta gtgaaggtgc
     1921 accggaaatt aaagatttaa cattagatat aggtgctcaa aatgaagatg aggtgcgtga
     1981 gcgcggaata gatataggag atacaattgt acctcacacg ccattcacac agttatctga
     2041 acatcgatat agtgctaaag catgggataa tcgttatggt tgtgtcttgg caattgaaat
     2101 actagaatta ttaaaagata tagaattaga tgtagacttg tatgttggcg caaatgttca
     2161 agaagaggtt ggattacgag gtgcgaaagc atctgcagag atgatagacc cagacgttgc
     2221 atttgtagtt gattgttcac ctgccaatga cgttaaagga aaccaaccat tatctggtga
     2281 acttggtaaa gggacgttaa ttcgcataaa agacggtaca atgattttaa agcctgtatt
     2341 tagagactat ttattaaagt tagtagaagc acatgacatt gaacatcaat actatatgtc
     2401 accaggtgga acagatggtg gagaaattca taaagctaat attggtattc cgactgcagt
     2461 tattggtgta tgtgcacgat atattcatag tacagactca gtatttgata taagagacta
     2521 ttttgcagct agatctttac tttcagaagc catttgtaat ttagataata atcaaataga
     2581 aacattacaa tataaataat cgggtaataa caactattat ctctaaatag ttatatataa
     2641 tcattaatta aggagacata aaaatgaaac aacttgaatc agaacaacaa tttgaatctt
     2701 taaaacaagg tgctacagta tttgaattca ctgcaggctg gtgtccagat tgtagagtga
     2761 tagaaccaga tttaccggaa ttagaagcga gatatcctat gtttgacttc gtatcagtag
     2821 accgtgataa atttatggat atttgtattg aaaatggtat tatgggtatt ccaagttttc
     2881 tagtatataa aaatggagaa ctgcttggaa gttatattgg aaaagaacga aaatcaattg
     2941 aacagataga tgcattttta gctcaatacg tgtaatttag actagagaaa aacggggtaa
     3001 tactcgtttt tctctgttac tatgtgttga tttattgtaa actattataa ggtgcgaaat
     3061 taggagtgtt acatatgaat acctttcaaa tgagagataa attaaaggaa cgtttaagcc
     3121 atttagacgt tgattttaaa tttaatcgtg aagaagaaac tttgcgtatt tatcgaacag
     3181 ataataacaa aggtatcacg attaaactta acgctatagt cgcaaaatat gaagataaaa
     3241 aagaaaaaat tgtagatgaa attgtttatt acgttgatga agctattgca caaatggcag
     3301 ataaaaccct tgagagtata tcatctagtc aaattatgcc tgtcattaga gcgactagct
     3361 ttgataaaaa aactaaacaa ggtgttcctt ttatctatga tgagcatact gcagaaacag
     3421 cagtttatta tgcagtcgat ttagggaaat catatcgtct aattgacgaa agcatgttag
     3481 aggatttgaa gttaactgaa caacaaataa gagaaatgtc tctgtttaat gttagaaaat
     3541 tgtcaaattc atatacgact gatgaagtaa aaggtaatat tttttatttt attaactcaa
     3601 atgacgggta tgatgcaagt aggatactaa atactgcatt tttaaatgaa attgaggcac
     3661 aatgtcaagg cgaaatgctc gtagcagtgc cacaccaaga tgtgttaatt attgcagata
     3721 tacgcaataa aacaggatat gatgtgatgg cacatttaac aatggaattt ttcactaaag
     3781 gtctagttcc aattacatca ttatcctttg gatataaaca gggtcatctt gaaccgatat
     3841 ttattttagg taaaaataat aaacaaaaaa gagatccaaa cgtgattcag cgtttagaag
     3901 caaatcgtcg taaatttaat aaagataaat agaaataatt ggataaggag ttttgtcata
     3961 atgaatttat tttacaatcc taaatatgta ggagatgtcg catttttaca aattgaacca
     4021 gttgaaggtg aattaaacta caataaaaaa ggtaatgttg ttgaaattac taatgaaggt
     4081 aatgttgtag gttataatat ttttgaaatt tcaaaagata taacaattga agaaaaaggt
     4141 catattaaat taactgatga acttgtaaat gtattccaaa agcgtatttc agaagctggt
     4201 tttgattata aattaaatgc tgatctatca ccgaaatttg tagttggcta cgttgaaact
     4261 aaagacaaac atcctgatgc agataaatta agtgtactaa atgtaaacgt tggaaatgac
     4321 acattacaaa ttgtatgtgg cgcgcctaac gttgaagctg gacagaaagt tgttgttgct
     4381 aaagtaggtg cagtgatgcc tagcggtatg gtaattaaag atgctgaatt acgtggtgtt
     4441 gcctcaagcg gtatgatttg ttcaatgaaa gaattgaatt tacctaatgc acctgaagaa
     4501 aaaggtatta tggtattaaa tgacagctat gaaattggac aagcattttt tgaataatta
     4561 aggaaggtag tgaaaatatg agctggtttg ataaattatt cggcgaagat aatgattcaa
     4621 atgatgactt gattcataga aagaaaaaaa gacgtcaaga atcacaaaat atagataacg
     4681 atcatgactc attactgcct caaaataatg atatttatag tcgtccgagg ggaaaattcc
     4741 gttttcctat gagcgtagct tatgaaaatg aaaatgttga acaatctgca gatactattt
     4801 cagatgaaaa agaacaatac catcgagact atcgcaaaca aagccacgat tctcgttcac
     4861 aaaaacgaca tcgccgtaga agaaatcaaa caactgaaga acaaaattat agtgaacaac
     4921 gtgggaattc taaaatatca cagcaaagta taaaatataa agatcattca cattaccata
     4981 cgaataagcc aggtacatat gtttctgcaa ttaatggtat tgagaaggaa acgcacaagc
     5041 caaaaacaca taatatgtat tctaataata caaatcatcg tgctaaagat tcaactccag
     5101 attatcacaa agaaagtttc aagacttcag aggtaccgtc agctattttt ggcacaatga
     5161 aacctaaaaa gttagaaaat ggtcgtatcc ctgtaagtaa accttcagaa aaagttgagt
     5221 cagataaaca aaaatatgat aaatatgtag ctaagacgca aacgtctcaa aataaacaat
     5281 tagaacaaga aaaacaaaat gatagtgttg tcaaacaagg aactgcatct aaatcatctg
     5341 atgaaaatgt atcatcaaca acaaaatcaa tgcctaatta ttcaaaagtt gataatacta
     5401 tcaaaattga aaatatttat gcttcacaaa ttgttgaaga aattagacgt gaacgagaac
     5461 gtaaagtgct tcaaaagcgt cgatttaaaa aagcgttgca acaaaagcgt gaagaacata
     5521 aaaacgaaga gcaagatgca atacaacgtg caattgatga aatgtatgct aaacaagcgg
     5581 aacgctatgt tggtgatagt tcattaaatg atgatagtga cttaacagat aatagtgact
     5641 taacagataa tagtacagat gctagtcagc ttcatacaaa tggcatagag aatgaaactg
     5701 tatcaaatga tgaaaataaa caagcgtcaa tacaaaatga agacactaat gacactcatg
     5761 tagatgaaag tccatacaat tatgaggaag ttagtttgaa tcaagtatcg acaacaaaac
     5821 aattgtcaga tgatgaagtt acggtttcga atgtaacgtc tcaacatcaa tcagcactac
     5881 aacataacgt tgaagtaaat gataaagatg aactaaaaaa tcaatccaga ttaattgctg
     5941 attcagaaga agatggagca acgaataaag aagaatattc aggaagtcaa atcgatgatg
     6001 cagaatttta tgaattaaat gatacagaag tagatgagga tactacttca aatatcgaag
     6061 ataataccaa tagaaacgcg tctgaaatgc atgtagacgc tcctaaaacg caagagtacg
     6121 cagtaactga atctcaagta aataatatcg ataaaacggt tgataatgaa attgaattag
     6181 caccgcgtca taaaaaagat gaccaaacaa acttaagtgt caactcattg aaaacgaatg
     6241 atgtgaatga taatcatgtt gtggaagatt caagcatgaa tgaaatagaa aagaataacg
     6301 cagaaattac agaaaatgtg caaaacgaag cagctgaaag tgaacaaaat gtcgaagaga
     6361 aaactattga aaacgtaaat ccaaagaaac agactgaaaa ggtttcaact ttaagtaaaa
     6421 gaccatttaa tgttgtcatg acgccatctg ataaaaagcg tatgatggat cgtaaaaagc
     6481 attcaaaagt caatgtgcct gaattaaagc ctgtacaaag taagcaagct gtgagtgaaa
     6541 gaatgcctgc gagtcaagcc acaccatcat caagatctga ttcacaagag tcaaatacaa
     6601 atgcatataa aacaaataat atgacatcaa acaatgttga gaacaatcaa cttattggtc
     6661 atgcagaaac agaaaatgat tatcaaaatg cacaacaata ttcagagcag aaaccttctg
     6721 ttgattcaac tcaaacggaa atatttgaag aaagtcaaga tgataatcaa ttggaaaatg
     6781 agcaagttga tcaatcaact tcgtcttcag tttcagaagt aagcgacata actgaagaaa
     6841 gcgaagaaac aacacatcca aacaatacta gtggacaaca agataatgat gatcaacaaa
     6901 aagatttaca gtcatcattt tcaaataaaa atgaagatac agctaatgaa aatagacctc
     6961 ggacgaacca acaagatgtt gcaacaaatc aagctgtaca aacatctaag ccgatgattc
     7021 gtaaaggccc aaatattaaa ttgccaagtg tttcattact agaagaacca caagttattg
     7081 agtcggacga ggactggatt acagataaaa agaaagaact gaatgacgca ttattttact
     7141 ttaatgtacc tgcagaagta caagatgtaa ctgaaggtcc aagtgttaca agatttgaat
     7201 tatcagttga aaaaggtgtt aaagtttcaa gaattacggc attacaagat gacattaaaa
     7261 tggcattggc agcgaaagat attcgtatag aagcgcctat tccaggaact agtcgtgttg
     7321 gtattgaagt tccgaaccaa aatccaacga cagtcaactt acgttctatt attgaatctc
     7381 caagttttaa aaatgctgaa tctaaattaa cagttgcgat ggggtataga attaataatg
     7441 aaccattact tatggatatt gctaaaacgc cacacgcact aattgcaggt gcaactggat
     7501 cagggaaatc agtttgtatc aatagtattt tgatgtcttt actatataaa aatcatcctg
     7561 aggaattaag attattactt atcgatccaa aaatggttga attagctcct tataatggtt
     7621 tgccacattt agttgcaccg gtaattacag atgtcaaagc agctacacag agtttaaaat
     7681 gggccgtaga agaaatggaa cgacgttata agttatttgc acattaccat gtacgtaata
     7741 taacagcatt taacaaaaaa gcaccatatg atgaaagaat gccaaaaatt gtcattgtaa
     7801 ttgatgagtt ggctgattta atgatgatgg ctccgcaaga agttgaacag tctattgcta
     7861 gaattgctca aaaagcgaga gcatgtggta ttcatatgtt agtagctacg caaagaccat
     7921 ctgtcaatgt aattacaggt ttaattaaag ccaacatacc aacaagaatt gcatttatgg
     7981 tatcatcaag tgtagattcg agaacgatat tagacagtgg tggagcagaa cgcttgttag
     8041 gatatggcga tatgttatat cttggtagcg gtatgaataa accgattaga gttcaaggta
     8101 catttgtttc tgatgacgaa attgatgatg ttgttgattt tatcaaacaa caaagagaac
     8161 cggactatct atttgaagaa aaagaattgt tgaaaaaaac acaaacacaa tcacaagatg
     8221 aattatttga tgatgtttgt gcatttatgg ttaatgaagg acatatttca acatcattaa
     8281 tccaaagaca tttccaaatt ggctataata gagcagcaag aattatcgat caattagagc
     8341 aactcggtta tgtttcgagt gctaatggtt caaaaccaag ggatgtttat gttacggaag
     8401 cagatttaaa taaagaataa ttatgagtaa ggagttttat ataatgacac actatcattt
     8461 tgtcggaatt aaaggttctg gcatgagttc attagcacaa atcatgcatg atttaggaca
     8521 tgaagttcaa ggatcggata ttgagaacta cgtatttaca gaagttgctc ttagaaataa
     8581 ggggataaaa atattaccat ttgatgctaa taacataaaa gaagatatgg tagttataca
     8641 aggtaatgca ttcgcgagta gccatgaaga aatagtacgt gcacatcaat tgaaattaga
     8701 tgttgtaagt tataatgatt ttttaggaca gattattgat caatatactt cagtagctgt
     8761 aactggtgca catggtaaaa cttctacaac aggtttatta tcacatgtta tgaatggtga
     8821 taaaaagact tcatttttaa ttggtgatgg cacaggtatg ggattgcctg aaagtgatta
     8881 tttcgctttt gaggcatgtg aatatagacg tcacttttta agttataaac ctgattacgc
     8941 aattatgaca aatattgatt tcgatcatcc tgattatttt aaagatatta atgatgtttt
     9001 tgatgcattc caagaaatgg cacataatgt taaaaaaggt attattgctt ggggtgatga
     9061 tgaacatcta cgtaaaattg aagcagatgt tccaatttat tattatggat ttaaagattc
     9121 ggatgacatt tatgctcaaa atattcaaat tacggataaa ggtactgctt ttgatgtgta
     9181 tgtggatggt gagttttatg atcacttcct gtctccacaa tatggtgacc atacagtttt
     9241 aaatgcatta gctgtaattg cgattagtta tttagagaag ctagatgtta caaatattaa
     9301 agaagcatta gaaacgtttg gtggtgttaa acgtcgtttc aatgaaacta caattgcaaa
     9361 tcaagttatt gtagatgatt atgcacacca tccaagagaa attagtgcta caattgaaac
     9421 agcacgaaag aaatatccac ataaagaagt tgttgcagta tttcaaccac acactttctc
     9481 tagaacacag gcatttttaa atgaatttgc agaaagttta agtaaagcag atcgtgtatt
     9541 cttatgtgaa atttttggat caattagaga aaatactggc gcattaacga tacaagattt
     9601 aattgataaa attgaaggtg catcgttaat taatgaagat tctattaatg tattagaaca
     9661 atttgataat gctgttattt tatttatggg tgcaggtgat attcaaaaat tacaaaatgc
     9721 atatttagat aaattaggca tgaaaaatgc gttttaatat gtttataata gagtagtatg
     9781 ggtatttatt attaatgaca ttattacatg ttaattagga ggcgttttta atggattgga
     9841 ttttaccaat tgctggaatt atcgctgcga ttgcattctt aattttatgt atcggtatcg
     9901 tagctgtatt aaattctgtt aagaaaaact tagattatgt tgcaaaaaca cttgacggtg
     9961 tagaaggtca agttcaaggt attactcgtg aaacaacaga tttacttcat aaagtaaacc
    10021 gtttaactga ggatatccaa ggtaaagtag atcgtttaaa ctcagttgta gatgctgtta
    10081 aaggtatcgg tgactcagta caaacgttaa acagctctgt agatcgtgta acaaattcaa
    10141 ttacacataa tatttctcaa aatgaagata aaatctcaca agttgttcaa tggtcaaatg
    10201 ttgcaatgga aattgcagac aaatggcaaa atagacacta ccgtcgtgga agtgcaaatt
    10261 acaaagctaa taatgtagca actgatgcaa atcatagcta tacttctaga gtagataaat
    10321 aattaataat taaacaaatg cacttaaatg aggttgttac ttatttaagt gcatttgtta
    10381 taggaggata tacaaatgac gcagcaacaa aatgataaaa gaacattaaa aaataaacac
    10441 acttatcaaa atgaaccact accaaaccgt aaagattttg ttgttagttt tataactggc
    10501 gcgcttgttg gttcagcttt aggtttgtat tttaaaaata aagtttatca aaaagcagat
    10561 gatttaaaag tcaaagaaca agaactgtcg caaaagtttg aagaaagaaa aacgcaactt
    10621 gaagaaacgg ttgcctatac aaaagaacgt gttgaaggat ttttaaacaa atctaaaaat
    10681 gagcaagcgg cattaaaggc acaacaagca gcgataaaag aagaagcaag tgcaaataat
    10741 ttaagtgata catcacaaga agcacaagag attcaagaag ctaaaagaga agcacaagca
    10801 gaagcggata aaagtgtggc tgtatcaaat aaagaatcaa aagcagtggc attgaaagca
    10861 caacaagcag cgataaaaga agaagcaagt gcaaataatt tgagtgatac atcacaagag
    10921 gcacaagag
//
LOCUS       DAXWFK010000088        10782 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.088, whole genome shotgun sequence.
ACCESSION   DAXWFK010000088 DAXWFK010000000
VERSION     DAXWFK010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10782
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.088"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            212..976
                     /locus_tag="ACGEXS_001699"
     CDS             212..976
                     /locus_tag="ACGEXS_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFF8012700.1"
                     /translation="MFLDEHINRNFDKLNDNDLHIAHFINTHIDECKNMKIQDLAQFT
                     HASNATIHRFTRKLGFDGYSDFKSYLKFESNKTHQLPSNSIDSFKQEIDSTFSYLERV
                     DYQLLTKKMHTASTIYLYGTGRAQLNVASEAQRILLTLHNHVIVLHDEHELKMILNKS
                     EPNDLFFIISLSGETHELLEVTNLLQLRQKYFISVTTMKDNTLAQKANYNVYVSSNTF
                     YLTDGTDYSSFISYHIFFETLVRKYNEYLLHNDISN"
     gene            complement(1034..1540)
                     /locus_tag="ACGEXS_001700"
     CDS             complement(1034..1540)
                     /locus_tag="ACGEXS_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HFF8012701.1"
                     /translation="MAKFNVENEHVEVEIEKLYKFSPELVYEAWTKKDLLKQWFMTSA
                     RTNKEIEADVKEGGKYRIVDQQRNGKVNVIEGIYESLVMDEYVKMTIGMPGLSETQDV
                     IEVEFFERETGGTQMLFYYRSLVEKERRFTNLEYKQKKKEYHDAMVHGFELMFDKMYH
                     VIETSTQQ"
     gene            complement(1707..3020)
                     /locus_tag="ACGEXS_001701"
     CDS             complement(1707..3020)
                     /locus_tag="ACGEXS_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HFF8012702.1"
                     /translation="MEVHKKSNAWALFPLLLFVALFLGVGIITGDFTSMPLNVAITIT
                     VIVALLMNRKESFAKKVEVFTKGAGHSNIVLMMLIFILAGAFSNTAEKMGGVKSTVNL
                     GLSLIPENLIIVGLFVICMFVSISMGTSVGTVAAIAPVGYGFAQATDVPTALAMATVV
                     GGAMFGDNLSMISDTTIAAVRTQHTKMKDKFRVNFKIVLPGAILTIIILYFLTNGISL
                     NHAKNYDYNLIKVVPYILVLVLALLGVNVIIVLIGGTLLAGIIGLIDGSFGWMGLLDA
                     VSKGIISMEDIAMIALLIGGLVGIIQHNGGIEWLLQFVRSKVKSKRGAELGIASLVSV
                     ADIATANNTISIIMSGPLAKNIADEYDVDSRKSASILDIFGGCFQGLLPYSPQVISAA
                     GVAGISPLMLFPYSIYPILLGVCGLIAILFNFPKLKKNTSQEMKH"
     regulatory      complement(3097..3192)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(3245..3814)
                     /locus_tag="ACGEXS_001702"
     CDS             complement(3245..3814)
                     /locus_tag="ACGEXS_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012703.1"
                     /translation="MALIVSRAILFLILALAFGIATVFPEQKLPLYIVFTLFVAVAIV
                     SNVWFTHQNVKKHIIKFNKYVAYFLSFVSGLLYIGFVIYTLTIGSYDVLPIIIFAISI
                     VLVQILENILGISIKNTRLSNKQLKVISKQRVQQFKVISLILVSVSIVFIFIALMLKW
                     WLLLVISIIVLIIITVAMIVYEKYYTTPD"
     gene            complement(4301..5182)
                     /locus_tag="ACGEXS_001703"
     CDS             complement(4301..5182)
                     /locus_tag="ACGEXS_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFF8012704.1"
                     /translation="MAAQDPRTKFKTTDYEKQEQEVPGLQSEMTPAPDCGETSYQGHQ
                     RLQGYKILVTGGDSAIGRAAAIAYAKEGADVAINYLPSEEQDAQEVRQVIEESGQKAV
                     LIPGDIRDEQFNYDLVEQAYQQLGGLDNVTLVAGHQQYHDDIHGFTTEAFTETFETNV
                     YPLFWTVQKALEYLKPGASITTTSSVQGYNPSPILHDYAASKAAIISLTKSFSEELGP
                     KGIRVNCVAPGPFWSPLQISGGQPQSKIPTFGQKTPLGRAGQPVELCGTYVLLASEES
                     SYTTGQVFGVSGGVQID"
     gene            complement(5293..6414)
                     /locus_tag="ACGEXS_001704"
     CDS             complement(5293..6414)
                     /locus_tag="ACGEXS_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HFF8012705.1"
                     /translation="MGLKDQAVTWRRYFHQFPELSDKEFKTTQKIKDILTEHHIRILD
                     LPLATGLVAEVGQGLSCIAVRADIDALPIQELVEQDFKSENEGVMHACGHDIHMASIL
                     ATAVKLKEIEGTLTGRVKFIFQSAEELGHGAFKIIETHALKDVQAVLGFHNDPSRSVG
                     TFAIKTGAITSAVDRFEFHIKGVGGHAAKPEQCNDPVIVLAQLINSIQSIVSRNLSAF
                     DEAVVTIGQISCGNTWNVIADHAYVQGTVRSFDPVVRKLVETRLQDIADGLAQAYNMK
                     INLNYTHLPGAVMNDEALTHKAIAVAQHVGYKVEMMEQPLTIGEDFSGYSQHFPSVFA
                     LIGSHSEYDLHHPQYKPDERILEKVPEYFVEFVKRLLHE"
     gene            complement(6691..7929)
                     /gene="hutI"
                     /locus_tag="ACGEXS_001705"
     CDS             complement(6691..7929)
                     /gene="hutI"
                     /locus_tag="ACGEXS_001705"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0019556 - L-histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HFF8012706.1"
                     /translation="MNDLIINHIAELILPRSTDKPLKGKELDELNVVKNGTVVIKDGK
                     IVYAGTHTDDYDATETIDASGKVVSPALVDAHTHLTFGGSREHEMSLKRQGKSYLEIL
                     EMGGGILSTVNATRETSEDDLFKKAEHDLLTMIKHGVLAVESKSGYGLDRENELKQLK
                     VSNRLAEKYDLDMKHTFLGPHAVPKEASSNEAFLEEMIALLPEVKQYADFADIFCETG
                     VFTIEQSQHYMQKAKEAGFKVKIHADEIDPLGGLELAIDEQAISADHLVASSDKGKEK
                     LRNSDTVAVLLPATTFYLGKEDYADARGMLDNNGAIALATDYNPGSSVTNNLQLVMAI
                     AALKLKLSPNEVWNAVTVNAAKAIDINAGTINTGDKANLVIWDAPNHEYIPYHFGINH
                     AEKVIKDGKVIVDNTVSFKA"
     gene            complement(7929..9590)
                     /gene="hutU"
                     /locus_tag="ACGEXS_001706"
     CDS             complement(7929..9590)
                     /gene="hutU"
                     /locus_tag="ACGEXS_001706"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HFF8012707.1"
                     /translation="MRKIQAKKGLSIECKGWEQEAVLRMLYNNLDPEVAERPEDLVVY
                     GGIGKAARNWEAFEAIEKTLRELESDETMLVQSGKPVAVFKTHEEAPRVLISNSVLVP
                     EWANWDHFNELDKKGLIMYGQMTAGSWIYIGSQGIVQGTYETFAELGNQHFNGDLAGT
                     VTLTAGLGGMGGAQPLAITMNHGVAICVDVDETRVDKRIDTKYCDVKTADLDEALKLA
                     EEAKERGEGLSIGLVGNAVDIHQAILEKGFKIDIITDQTSAHDPLNGYVPQGYSVEEA
                     KVLREKDPKKYVELSQASMAKHVELMLEFQKRGAVAFDYGNNIRQVAFNNGVKNAFDF
                     PGFVPAYIRPLFCEGKGPFRFAALSGDPKDIERADEEMRKLFPENEKLLRWLDLAEEK
                     ISYQGLPSRIAWLGYGERAKMGLALNRLVRDGEISAPIVIGRDHLDAGSVASPNRETE
                     SMKDGSDAVGDWAVLNALINTAAGGSWISFHHGGGVGMGYSLHAGMVVVADGSERAER
                     RLERVLTTDPGMGVARHVDAGYDIAIQTAKEKGIHIPMIDKAGDK"
     gene            9750..10634
                     /locus_tag="ACGEXS_001707"
     CDS             9750..10634
                     /locus_tag="ACGEXS_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFF8012708.1"
                     /translation="MKIIQLEYFLAIVKYNSFTKAAQFLHISQPSLTATIKKMEADLG
                     YDLFTRSTKDIKITEKGIQFYRYASELVQQYRSTMEKMYDLSVTSEPRIKIGTLESTN
                     QWIANLIRKHHSDYPEQQYRLYEIHDKHQSIEQLLNFNIHLAITNEKITHEDIRSIPL
                     YEESYILLAPKETFKNQNWVDVENLPLILPNKNSQVRKHLDDYFNRRNIRPNVVVETD
                     RFESAVGFVHLGLGYAIIPRFYYQSFHTSNLEYKKIRPNLGRKIYINYHKKRKHSEQV
                     HTFVQQCQDYLYGLLEAL"
BASE COUNT         3403 a         2218 c         1525 g         3636 t
ORIGIN      
        1 tgacgtattt tgaaagtatt ttgtataatc agattatctt ttcatatagt gaaaattttt
       61 tcacgacctt atatatgaca tcgttgtatt tgtaatacat tcgttttaaa cgcataatca
      121 aacctatatc aatacacaaa tatatataat gacatacaag attttaatgt aataacgatc
      181 tattacacat ttattttcaa ggaggttgaa tatgttttta gatgaacaca ttaatcgaaa
      241 ctttgataaa cttaatgata atgatttaca tatcgctcac tttatcaata cacatataga
      301 tgaatgtaaa aatatgaaaa tacaagattt agcgcaattc acacatgcct cgaatgcaac
      361 cattcacaga tttacacgca aattaggttt tgacggttat agtgatttta aatcgtacct
      421 taaatttgaa agtaataaga cacaccaatt accttctaac tctatcgata gtttcaaaca
      481 agaaatcgat agtacattca gttatttaga acgcgttgat taccagcttc ttactaaaaa
      541 aatgcataca gcttcaacaa tatatttgta tggtacaggg cgtgcacagt taaacgttgc
      601 ttcagaggca cagcgtatat tgttaacgtt acataatcat gtcattgtac ttcatgatga
      661 acatgaactt aaaatgattt tgaacaagag tgaacctaat gatttattct ttattatttc
      721 attatcaggt gaaacacatg aattattaga agtaacaaac ttgcttcaac ttagacaaaa
      781 atactttatt tctgttacta caatgaaaga taataccctt gctcaaaaag caaattacaa
      841 cgtctatgta tccagtaaca cattctactt aactgatggt acagactatt ctagcttcat
      901 cagttatcat atattctttg aaacacttgt aagaaaatat aatgagtatt tactacataa
      961 tgatatttca aattagaaaa aaggaagtta tgatttaatg gccttgagcc tatcataact
     1021 tccttttatc attttattgt tgtgttgatg tttcgataac gtggtacatc ttatcaaaca
     1081 tcaattcgaa accatgcacc atggcatcat gatattcttt tttcttttgc ttgtattcta
     1141 aattagtaaa tcgtctttct ttttcaacta atgaacgata ataaaatagc atttgggtgc
     1201 cacctgtttc acgttcaaaa aattctacct caatgacatc ttgcgtttca cttagtccag
     1261 gcataccgat agtcatctta acgtattcat ccataactaa agattcataa atgccttcaa
     1321 tcacatttac tttgccatta cgttgttgat ctacaatacg atatttaccg ccttctttaa
     1381 cgtccgcttc aatctcttta ttcgttctgg ctgatgtcat aaaccattgt ttcaacaaat
     1441 ctttctttgt ccaagcttcg tatactaact ctggagaaaa tttataaagc ttttcaattt
     1501 caacttcgac atgttcattc tctacattaa attttgccac tgttgtccac ccactttcgc
     1561 tcttactttt attttaacgt atttttgctc agttccaaac atagatgatc atcattttta
     1621 aaagattagc gttatacggt gagtacaaca tgatctgtta atataacaag ccaccttact
     1681 tggctacatc gatatattgt taagcattaa tgtttcattt cttgactagt gttctttttt
     1741 agctttggaa aattaaataa aatcgcaata agtccgcata cacctaataa tataggataa
     1801 atgctgtatg ggaataacat taacggtgaa ataccagcta caccagccgc tgaaatgact
     1861 tgcgggctat atggtaataa accttggaag cagcctccaa atatatcaag aatacttgct
     1921 gatttccttg aatctacatc atattcatct gcaatatttt tagctaaagg acctgacata
     1981 ataatagaga tggtgttgtt tgccgtggca atatctgcga cacttaccaa actagcaatt
     2041 cctaattctg cgccacgctt tgatttcact ttagagcgaa caaattgcaa caaccattca
     2101 ataccaccat tgtgttgaat aataccgact aaaccaccaa ttagcaacgc aatcatagca
     2161 atatcttcca tgcttataat acctttggac actgcatcta gtagccccat ccaaccgaat
     2221 gaaccatcta tgagaccaat gattccggct aataatgttc cgccaatcaa tacgataatg
     2281 acatttacac ctaataatgc taataccaat actaagatat acggtacaac tttaattaga
     2341 ttataatcat agtttttagc atgatttaaa gaaatgccat tcgttaagaa atacagaata
     2401 ataatcgtta aaatagcacc tggcaataca attttaaagt ttactctgaa tttatctttc
     2461 attttcgtat gttgtgttct aaccgcagca attgttgtat ctgaaatcat tgatagatta
     2521 tcgccgaaca ttgcacctcc aacaactgta gccattgcta gcgcagttgg tacatctgtc
     2581 gcttgtgcaa atccgtaacc aacaggtgct attgcagcaa cagttcctac agacgtcccc
     2641 atagatatag aaacaaacat acaaatcaca aacaatccta caataattaa attttctggg
     2701 attaatgata gtcctaaatt aactgtcgac tttacgccac ccattttttc agctgtattt
     2761 gaaaatgcac ctgctaaaat aaaaatcaac atcattaaaa caatgtttga atggcctgca
     2821 cctttcgtga agacctcaac ttttttagca aatgattctt ttcgattcat taataacgcc
     2881 acaattaccg ttatcgtaat tgcaacattt aatggcattg aagtaaaatc acctgtgata
     2941 atacctacgc ctaaaaacaa cgccacaaat aataacaagg ggaataatgc ccaagcattg
     3001 ctctttttat gtacttccat cctttttacc tgctttccaa ttaaaaatac ctctttctca
     3061 caaacgatga agaaagaggt tttcatgtgc tttacctgct tatcttcaaa ccattacggt
     3121 tactggaatt ggcacattcg agatgttgcc gaggcttcat agggccagtc cctccacctc
     3181 tctagataag tgatgcttat ttacgtttac gttacaagat aatccttagt acgtcaatca
     3241 taaattaatc aggagtcgta taatattttt cataaacaat cattgctact gtaataataa
     3301 tcaaaacaat aatgctaata acaagtaaaa gccaccattt aagcattaat gcaataaaaa
     3361 tgaacacgat agacacactt actaatatta atgatatgac tttaaattgc tgaacacgtt
     3421 gcttggagat gactttcaac tgtttgtttg atagacgcgt attttttata ctgattccca
     3481 gtatattttc taatatttga accaatacga tacttattgc aaatataata attggtaaaa
     3541 catcatagct ccctatagtt aatgtataaa ttacaaatcc aatgtaaagt aaccctgaga
     3601 caaaggataa aaagtatgcg acgtatttgt taaacttaat gatatgcttt ttaacgtttt
     3661 gatgtgtaaa ccatacattc gaaacgatcg caactgctac aaataatgtg aatactatat
     3721 ataatggtaa tttttgttca ggaaaaacag ttgctattcc aaaagctaat gctaaaatca
     3781 aaaataatat agctctagat actattaatg ccataataac aacccctttg tttaatatcg
     3841 agtttgcaaa tttacgttta tcagcgtttc tatgatcagt acttctacgg gtagcgtttc
     3901 tatgtaattt acatcatctt aacatataaa tacttcgcta tttaattgaa aacatatcct
     3961 attattcttt gtccgttctg acgtttaata tctagcctta ggcatttcac ttgttaatga
     4021 atttaacttt cttccactaa ccgtccctaa acccaatccc gcaacagttt ttaacttttt
     4081 cgttgttgtc ctgacatcct cattaagaaa gtttattctg cttaaaactt ataatccaca
     4141 ccctgagcaa acgctcctta tgacagagta ttaaaataag ccgataaaga tacacacctt
     4201 taccgactat ttaaaataca cttcaccaat tcattttaat ttaatggatt gaagtaacta
     4261 aattaatatt atgttgttca attaaaagct tcatacaaac ctaatctatt tgcactccac
     4321 cgctaacacc gaacacttgt ccggttgtat aacttgattc ttctgacgct aatagcacat
     4381 aagttccaca taactcaaca ggttgacctg cacgacctaa aggtgttttt tgaccaaatg
     4441 ttgggatttt actttgaggt tgtccaccag aaatttgtaa tggtgaccag aatggaccag
     4501 gcgctacaca gttcactcta attccttttg gtcctaattc ttctgaaaaa cttttagtta
     4561 atgaaataat tgctgctttt gaagcggcat aatcatgaag aataggacta ggattataac
     4621 cttgtacaga tgatgtcgtt gtaattgacg cacccggttt taaatattcc aatgcttttt
     4681 gaactgtcca aaatagcgga tagacattcg tttcaaatgt ttctgtaaat gcctcagttg
     4741 taaatccatg aatatcatca tgatactgtt gatgtccagc aactaaagta acattatcta
     4801 agccacctaa ttgttgatat gcttgttcaa caaggtcata gttgaactgt tcatctctta
     4861 tatcaccagg aattaacact gccttttgac cactttcttc aatcacttgg cgtacttctt
     4921 gtgcatcttg ttcttcactc ggaagatagt taatcgctac atctgcacct tctttagcat
     4981 acgcaattgc tgctgcacgc cctattgctg agtcaccacc tgtgactaat attttatagc
     5041 cttgtaagcg ttgatgacct tggtaagacg tttcgccaca atcgggtgct ggcgtcattt
     5101 cagattgtaa acccggtacc tcttgttctt gtttttcata atccgttgtt ttaaattttg
     5161 ttctaggatc ttgagctgcc atttttttac atctccttat tcgcttaatg gttattattt
     5221 acccaatctt cctaggaact taatcatgat tacactaaaa attactttct tctttataaa
     5281 aacaagctcg aattattcat gcaatagtct ctttacaaat tcaacaaaat actcaggtac
     5341 tttttccaga atcctttcat ccggtttata ttgaggatga tgtaaatcat attcactatg
     5401 agaaccaatt aacgcaaata cacttggaaa atgttgacta taacctgaaa aatcttctcc
     5461 aatcgtaagc ggctgttcca tcatttccac cttatatcca acatgttggg ctactgcaat
     5521 tgctttatgc gtcaatgcct catcattcat cacagcgcca ggtaaatgcg tataatttaa
     5581 attaattttc atattatatg cttgagccaa tccgtccgca atatcttgta atcgtgtttc
     5641 tacaagcttt cgtaccacag gatcaaaact acgcactgtg ccttgtacat acgcatgatc
     5701 agcaatgaca ttccaagtat taccacatga tatttgtcca attgttacta ccgcttcatc
     5761 aaacgcagat agatttctac taactatgga ttgaatacta ttaatcaatt gcgccaacac
     5821 aataactgga tcgttgcatt gttctggctt tgcagcatga ccacccacgc ctttaatatg
     5881 aaactcaaaa cgatctactg ctgatgtaat tgcccctgtt ttgattgcaa atgtacctac
     5941 cgaacgcgat gggtcattat gaaaacccaa tactgcttgt acatctttta atgcatgtgt
     6001 ttcaataatt ttaaaagcgc catgtcctag ttcttctgct gattgaaaaa tgaatttaac
     6061 acgcccagta agagtgccct caatttcttt taattttaca gctgtagcca aaatactagc
     6121 catgtgaata tcatgaccac acgcatgcat aacaccttca ttttcagatt taaaatcctg
     6181 ttcaacaagc tcttgaatcg gtaaagcatc aatgtcagcc cgaacagcta tacaactcag
     6241 accttgtccc acttcagcaa caagcccagt cgcaagtggt aagtctaata ttctaatatg
     6301 atgttctgtt aaaatatctt taattttttg tgtagtctta aattctttat cggatagttc
     6361 tggaaattga tgaaaatacc ttctccaggt aacagcttga tcttttaatc ccatcggtca
     6421 ttccccttcc ttaagtcaat gatatgttgt ctaccctacg atgatcatct ttgactatta
     6481 aacgatgatt tcacaacaat gtactcttgt taattgcttt cgttaatgat agacagttgt
     6541 ttaataatat cgtaacactg ttgtcaaact attctaactt ttataattga gactctatac
     6601 aaaaacgtgt tctcgaatat acttgttttt acaaaccaca aaaagctcta aacattagtt
     6661 taaaccaatg cttagagctt tctaattatt ttatgcttta aaagatactg tgttatctac
     6721 gatgacctta ccgtctttaa taactttttc tgcgtgattg ataccaaaat gatatggaat
     6781 atattcatga tttggtgcat cccaaattac taaattagcc ttatcacctg tgttaattgt
     6841 acccgcgtta atgtctattg ctttagcagc attgaccgta acagcattcc aaacttcatt
     6901 aggtgatagc tttaatttca aggctgcaat cgccataaca agttgtaagt tgtttgtgac
     6961 actactacca gggttataat cagttgctaa tgcaatcgca ccgttattgt caagcatgcc
     7021 tcttgcatct gcataatctt ctttacctaa atagaacgtc gttgcaggta agaggacagc
     7081 tacagtatca ctatttcgca acttttcttt tcctttatca ctagaagcta ctaagtggtc
     7141 tgctgatatt gcttgttcat caattgctaa ttccagtccg cctaacggat caatttcatc
     7201 cgcatgtatt ttcactttaa aacctgcttc tttggctttt tgcatataat gttgcgattg
     7261 ttctattgta aatacacctg tttcacagaa aatatccgca aagtctgcat attgttttac
     7321 ttccggaagt aacgcaatca tttcttctaa aaatgcctca tttgaacttg cctctttagg
     7381 tacagcatga ggccctagga aagtatgttt catgtctaaa tcatatttct cagctaaacg
     7441 attagacact ttcaattgct tcagttcatt ttctctatct aatccataac cactcttact
     7501 ttcaactgca agcacgccgt gtttaatcat agtaagcaaa tcatgctctg cttttttaaa
     7561 caagtcatct tcggatgttt ctctagtagc attaacggta gataatatgc caccacccat
     7621 ttctaatatt tcaaggtaag acttaccttg acgttttaat gacatctcat gttctcgaga
     7681 tccaccaaat gttaaatggg tatgtgcatc tactaatgct ggggacacta ccttcccact
     7741 agcatcaatc gtctcagtcg catcgtagtc atctgtatgt gttccagcat atacaatttt
     7801 gccatcttta atgacaactg taccattttt cacaacattt aattcatcta attccttacc
     7861 cttcaaaggt ttatctgttg atctcggtaa aattaattct gctatatgat taattattaa
     7921 atcattcatt acttatcacc tgctttatca atcattggaa tatgaatacc tttttcttta
     7981 gctgtttgaa tagcgatgtc atagccagca tcaacatgtc gggcaacacc catacctggg
     8041 tcagtcgtca atacacgttc caatcttctt tcagcacgct ctgatccatc tgctacaaca
     8101 accatacccg catgaagtga atatcccatg ccaacaccgc caccgtgatg gaatgaaatc
     8161 catgaaccac ctgcagctgt gttaatgagt gcattcaata cagcccaatc accaaccgcg
     8221 tcactaccat ctttcatact ttctgtttca cggttaggac tagcaactga accagcatct
     8281 aaatggtctc gtccaataac aattggtgct gaaatttcac cgtcacgtac aagacgattt
     8341 aaagctaagc ccattttcgc tctttctcca tagcctaacc aagcaatacg tgatggtagt
     8401 ccttgatatg aaattttttc ttcagctaaa tcaagccatc ttaataactt ttcattttct
     8461 gggaaaagtt tgcgcatttc ttcatccgca cgctcgatat cttttggatc accactcaac
     8521 gcagcaaagc ggaatggccc tttaccttca cagaataatg gtctaatgta agctggtaca
     8581 aagcctggga agtcaaaagc atttttcact ccgttattga aggctacttg acgaatattg
     8641 ttaccataat caaatgctac agcgccacgt ttttggaatt caagcattaa ttcaacatgc
     8701 tttgccattg aagcttgtga cagttcaaca tattttttcg gatctttttc acgcaatact
     8761 ttcgcttctt ctacagagta tccttgtggc acatatccat ttagcggatc atgtgcactt
     8821 gtttggtcag taataatgtc aattttaaat cctttttcta gaatcgcttg atggatgtct
     8881 acagcatttc caactaaccc gattgataat ccttctccac gttctttcgc ctcttctgct
     8941 aattttaatg cttcatctaa atcagctgtt ttaacatcac agtatttcgt atcaattcgc
     9001 ttatcaacac gtgtttcatc aacatccacg caaattgcta ccccatgatt catagtaatt
     9061 gctaacggtt gcgcaccacc cataccacct aaacctgctg tcagtgtaac agtgcctgct
     9121 aaatctccat taaagtgttg attacctagc tcggcaaatg tctcataagt accttgcaca
     9181 ataccttgag aaccaatata tatccaacta ccggctgtca tctgtccata catgattaaa
     9241 ccttttttat ctaattcatt aaaatgatcc cagtttgccc attcaggcac taatactgaa
     9301 tttgaaatta atacacgtgg cgcttcttca tgtgttttaa atacagcaac tggctttcct
     9361 gattgtacta acattgtctc atctgattct aattctcgta acgttttctc tattgcttca
     9421 aaagcttccc aattacgtgc tgcttttcca ataccaccat aaacaactaa atcttctggt
     9481 ctttcagcaa cttctgggtc taaattgttg tataacattc taagtactgc ttcttgttcc
     9541 caacctttac actcaatact caaacctttt tttgcttgaa tttttctcat aaaattcgct
     9601 cctgttcttt taagaagtta attccactaa atttaaaacg cttacattat tatcttcaat
     9661 attcattata gtatgttaaa atatagccaa caaatataaa taaactaatt atccatagct
     9721 tgaatctata aataaaagga gcaaaacaca tgaaaattat tcagttagaa tacttcttgg
     9781 ctatcgtgaa atataatagt tttactaaag ctgcacaatt tttacatatt agccagccat
     9841 ctttaactgc tacgattaaa aaaatggaag cagatttagg ttatgactta tttacacgtt
     9901 caacaaaaga catcaagatt accgaaaaag gaatacagtt ttatcgttat gcgagcgaat
     9961 tagttcaaca atatcgatcc acgatggaaa aaatgtatga tttaagcgtt acatcagaac
    10021 caaggataaa aattgggact cttgaatcta cgaatcaatg gattgcgaat ttaattcgaa
    10081 agcaccattc cgactaccct gaacagcaat atcgtttata tgaaatacat gataaacatc
    10141 aatctataga gcaattactg aattttaata ttcatttagc tataacaaat gaaaaaataa
    10201 cccacgaaga tataagatcc attcctttat atgaggaatc ttacatttta ttagcaccca
    10261 aggaaacatt taaaaatcaa aattgggtag atgttgaaaa tttgccactc atattaccaa
    10321 acaaaaattc tcaagtgcgc aaacacttag atgactattt taatagaaga aatattcgtc
    10381 caaatgtcgt tgtagaaaca gatcgattcg aatcagcagt tggatttgtt catctcggct
    10441 taggttacgc tatcattccg agattttatt accaatcatt tcacacgtct aatttagaat
    10501 ataaaaaaat tcgtccaaac ttaggccgaa aaatttatat caattaccat aaaaaacgca
    10561 aacactccga acaagtacat acattcgtac aacaatgcca agattattta tatggacttt
    10621 tagaggctct ttaacttaag ttattagagc ctcttatgca gttgctcagt caactgtata
    10681 cctttttcct ttaacttaag ttattagtgc ctcttatgca gttgctcagt caactgtata
    10741 cctttttcct ttaacttaag ttattagtgc ctcttatgca gt
//
LOCUS       DAXWFK010000089        10594 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.089, whole genome shotgun sequence.
ACCESSION   DAXWFK010000089 DAXWFK010000000
VERSION     DAXWFK010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10594
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.089"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            201..614
                     /locus_tag="ACGEXS_001708"
     CDS             201..614
                     /locus_tag="ACGEXS_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HFF8012709.1"
                     /translation="MPIVYYDGNCIYCYNYVIWLIQHDLPRNYQFATLKGEVGQQFFK
                     QHPEAANKNSVILQKGDRIYFESQAIIKLITALPNRTKLLGVGLWLVPKPVRNFGYRM
                     FANNRNRMWKTTWHQPNDYEKSFFLDDNAKVKLTD"
     gene            814..1257
                     /locus_tag="ACGEXS_001709"
     CDS             814..1257
                     /locus_tag="ACGEXS_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HFF8012710.1"
                     /translation="MSNQQDVVKELNQQVANWTVAYTKLHNFHWYVKGPNFFSLHVKF
                     EELYNEASQYVDELAERILAVGGNPVGTLTECLEQSIVKEAAKGYSAEQMVEELSQDF
                     TNISKQLENAIEIAGNAGDDVSEDMFIGMQTSVDKHNWMFKSYLS"
     gene            complement(1378..2088)
                     /gene="deoD"
                     /locus_tag="ACGEXS_001710"
     CDS             complement(1378..2088)
                     /gene="deoD"
                     /locus_tag="ACGEXS_001710"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFF8012711.1"
                     /translation="MTKGTPHIQPNGVKIAKTVLMPGDPLRAKYIADNFLENVEQFND
                     VRNMFGYTGTYKGKEVSVMGSGMGIPSIGIYSYELYNFFDVDTIIRIGSCGALQENVN
                     LYDVIIAQAASTNSNYVDQYNIPGHFAPITDFELVTKAKNVADQIGATTHVGNVLSSD
                     TFYNADPTFNDAWKKMGILGIEMESAGLYLNAIHAGKKALGIFTVSDHILRDEATTPE
                     ERQNSFTQMMEIALEIAE"
     gene            2403..3065
                     /gene="deoC"
                     /locus_tag="ACGEXS_001711"
     CDS             2403..3065
                     /gene="deoC"
                     /locus_tag="ACGEXS_001711"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFF8012712.1"
                     /translation="MNSAKLIDHTLLKPESTRTQIDQIIDEAKAYHFKSVCVNPTHVK
                     YAAERLADSEVLVCTVIGFPLGASTTATKAFETEDAIQNGADEIDMVINIGALKDGRF
                     DDVQQDIEAVVKAAKGHTVKVIIETVLLDHDEIVKASELTKAAGADFVKTSTGFAGGG
                     ATAEDVKLMKDTVGADIEVKASGGVRNLEDFNKMVEAGATRIGASAGVQIMQGLEADS
                     DY"
     gene            3344..4645
                     /locus_tag="ACGEXS_001712"
     CDS             3344..4645
                     /locus_tag="ACGEXS_001712"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFF8012713.1"
                     /translation="MRMIDIIEKKRDGHTLTTEEINFFIGGYVKGDIPDYQASSLAMA
                     IYFQDMNDDERAALTMAMVNSGDMIDLSDIKGVKVDKHSTGGVGDTTTLVLAPLVAAV
                     DVPVAKMSGRGLGHTGGTIDKLEAIDGFHVEIDEATFVKLVNENKVAVVGQSGNLTPA
                     DKKLYALRDVTGTVNSIPLIASSIMSKKIAAGADAIVLDVKTGSGAFMKTLEDAEALA
                     HAMVRIGNNVGRNTMAIISDMNQPLGRAIGNALELQEAIDTLKGQGPKDLTELVLTLG
                     SQMVVLANKAETLEEARALLIEAINSGAALEKFKTFIKNQGGDETVIDHPERLPQAQY
                     QIEYKAKKSGYVTELVSNDIGVASMMLGAGRLTKEDDIDLAVGIVLNKKIGDKVEEGE
                     SLLTIHSNRQDVDDVVKKLDSSITIADHVVSPTLIHKIITE"
     gene            4659..5009
                     /locus_tag="ACGEXS_001713"
     CDS             4659..5009
                     /locus_tag="ACGEXS_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012714.1"
                     /translation="MNILRYLLGIGFSVIGVLHFTRERQFRNIIPKCLPLRKTAVLVT
                     GIFEILFGLALLIKRPSQCLKNTINLFLLAVFPANIYMAVKKLPLGDKQLPTWQLYLR
                     LPMQFVLMGLIKKL"
     gene            complement(5161..5631)
                     /locus_tag="ACGEXS_001714"
     CDS             complement(5161..5631)
                     /locus_tag="ACGEXS_001714"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFF8012715.1"
                     /translation="MTKMNVESFNLDHTKVVAPFIRLAGTMEGLNGDVIHKYDIRFKQ
                     PNKEHMDMPGLHSLEHLMAENIRNHSDKVVDLSPMGCQTGFYVSFINHDNYDDVLNIV
                     EATLNDVLNATEVPACNEVQCGWAASHSLEGAKTIAQAFLDKRNEWHDVFGTGK"
     gene            5942..7126
                     /locus_tag="ACGEXS_001715"
     CDS             5942..7126
                     /locus_tag="ACGEXS_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFF8012716.1"
                     /translation="MSEKQQILDYIETNKYSYIEISHRIHERPELGNEEIFASRTLID
                     RLKEHDFEIETEIAGHATGFIATYDSGLDGPAIGFLAEYDALPGLGHACGHNIIGTAS
                     VLGAIGLKQVIDQIGGKVVVLGCPAEEGGENGSAKASYVKAGVIDQIDIALMIHPGNE
                     TYKTIDTLAVDVLDVKFYGKSAHASENADEALNALDAMISYFNGVAQLRQHIKKDQRV
                     HGVILDGGKAANIIPDYTHARFYTRAMTRKELDILTEKVNQIARGAAIQTGCDYEFGR
                     IQNGVNEFIKTPKLDDLFAKYAEEVGEAVIDDDFGYGSTDTGNVSHVVPTIHPHIKIG
                     SRNLVGHTHRFREAAASVHGDEALIKGAKIMALMGLELITNQDVYQDIIEEHAHLKGN
                     GK"
     gene            7126..8319
                     /locus_tag="ACGEXS_001716"
     CDS             7126..8319
                     /locus_tag="ACGEXS_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFF8012717.1"
                     /translation="MTNNGNEPNLTLSDLYDKDVVYTSRPSYISNPWLKPDEHQSNFL
                     TGRELLIANQLPVIVHEASATDKLHQLFQVIGKEVPNSIYTFNNQQSYENLIKQLAHK
                     ENKKIYFQYIHDETILNQQYYALDKTLFVALNNKARIPEWTNGKFLPKRKVVKIEQFE
                     NEIKNWEFPLVIKPGDDLPTAGGYGVMICYHDADLQKAITRIKEATAETNSLIIEQKI
                     EEKANYCVQFAYSESLGIQYLGAATQLTDKYGFYNGNENTTNVPEHVIEAGRQIMENG
                     VNQGFFGVAGFDLLVDEDDNVYAIDLNFRQNGSTSMLLLANELNSGYQKFYSYHSKGD
                     NTYFFNTILKYVKEGSLYPLSYYDGDWYGEDKVKSRFGCIWHGDSKETVLENERAFLA
                     ELEHY"
     gene            8718..9389
                     /locus_tag="ACGEXS_001717"
     CDS             8718..9389
                     /locus_tag="ACGEXS_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2750 domain-containing protein"
                     /protein_id="HFF8012718.1"
                     /translation="MSYKAYPFFRDILINECIYFASKNKKLVRLNYKSEAYVGVWTEE
                     SVAVSFLTSRDIPFDKVVKMDVDRFATYELDELFDEQDHIIMNQTMEEEGHLLNVVAV
                     TQEVMTELDKIRIKEFVQDVAKYDEVYGLTKKGSKQFILISENDSDEKKPHIMPVWSI
                     KNRALKVRDEDFEECDLITIEGSVFGEWLDELRDDHKAVAIDLKTGVVGTIVSAQKLS
                     NELTF"
     gene            complement(9519..10322)
                     /gene="coaW"
                     /locus_tag="ACGEXS_001718"
     CDS             complement(9519..10322)
                     /gene="coaW"
                     /locus_tag="ACGEXS_001718"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II pantothenate kinase"
                     /protein_id="HFF8012719.1"
                     /translation="MKVGIDAGGTLIKIVQEQDNQRTFKTELTKNIDQVVEWLNQQQI
                     EKLCLTGGNAGVIAENINIPAQIFVEFDAASQGLGILLKEQGHDLADYIFANVGTGTS
                     LHYFDGQSQRRVGGIGTGGGMIQGLGYLLSQITDYKQLTDMAQHGDRNTIDLKVRHIY
                     KDTEPPIPGDLTAANFGHVLHHLDADFTPSNKLAAVIGVVGEVVTTMAITVAREFKTE
                     NIVYIGSSFHNNALLRKVVEDYTVLRGCKPYYVENGAFSGAIGALYLEK"
BASE COUNT         3729 a         1504 c         2035 g         3326 t
ORIGIN      
        1 gttaataata agaaataaga ttacactgta gattgagaaa tttaaagtaa ataaaaaata
       61 cattatacaa taaatataaa actgaaaaat aaattttatg gcaatttcgt gtcgcgagga
      121 cttatcaagt gacaaccttg tttaaatgtt tttaaattag gaaaaatgac tataagtact
      181 attaaaggag cgaataaatt atgccaatcg tatattatga tggaaactgt atatattgtt
      241 ataactatgt catttggtta attcaacatg acctaccaag aaattatcaa tttgccacgt
      301 taaaaggtga agttggccaa caatttttta aacaacatcc agaagcggca aataaaaata
      361 gtgtgatttt acagaaaggt gatcgtatat attttgaatc gcaggcgatt attaaactaa
      421 tcacagcact acctaatcga actaaattat tgggcgtagg tttatggctt gtacctaaac
      481 cagtccgtaa tttcggttat cgaatgtttg ctaataatag aaatagaatg tggaaaacaa
      541 catggcacca accaaatgat tatgaaaaat cgttcttttt agatgataat gcgaaagtaa
      601 aacttactga ttgataaaac atacttgcta attgataatg gatatattag atgatgaatt
      661 aaaatttagg catttaaata gcggaacact ttacgattag attagaataa ttataaaaaa
      721 gagagtaaaa acactttaca gattagaatt attataatat aataattaat ataaacaagc
      781 aagacgtaga caattttaag gagtgtatta aatatgagta atcaacaaga tgttgtaaaa
      841 gaattgaatc aacaagtagc aaactggaca gtagcttaca caaagctaca caatttccac
      901 tggtatgtga aagggcctaa cttcttctca ttacacgtta aatttgaaga attatataat
      961 gaagcaagcc aatatgtaga cgaattagct gaaagaattt tagcggtagg aggaaaccct
     1021 gtaggtacat taactgaatg cttagagcaa tcaattgtta aagaagctgc gaaaggttac
     1081 tctgcagaac aaatggttga agaattatca caagacttca caaatatctc aaaacaatta
     1141 gaaaacgcaa tcgaaattgc tggtaatgct ggcgatgatg tatcagaaga tatgtttata
     1201 ggtatgcaaa catcagtaga taaacataat tggatgttta aatcttactt aagctaatct
     1261 acagataagt aataatataa ataaaagcca acacataaat ggggatacgc ccagaatgtg
     1321 ttggcttttt gagtttaatc gcattgttaa agagcaatta aagtcaattt aaataagtta
     1381 ctctgcgatt tctaaagcga tttccatcat ttgtgtaaat gaattttgac gttcttcagg
     1441 tgtagtagct tcgtcacgta aaatatgatc acttactgtg aaaataccaa gtgctttttt
     1501 accagcatga atcgcattta aatataaacc agctgattcc atttcgatac ctaaaatacc
     1561 catttttttc caagcatcat tgaatgttgg atcggcattg taaaatgtat cagaagataa
     1621 tacgttacct acgtgtgtag tagcaccgat ttggtcagcg acatttttag ctttagttac
     1681 taactcgaag tcagtgatag gcgcgaaatg acctggaata ttgtattgat ctacataatt
     1741 tgaattagtt gatgcagctt gtgcaataat aacatcgtat aagttaacat tttcttgtaa
     1801 tgcgccacaa gaaccgatac gaatgattgt atctacatca aagaagttgt ataactcata
     1861 tgagtaaata ccaatacttg gaatacccat accagaaccc attacagaaa cttctttacc
     1921 tttatatgta ccagtgtaac caaacatgtt acgtacatcg ttaaattgtt caacattttc
     1981 taaaaaatta tcagcaatat attttgcacg tagcggatcg ccaggcatta atactgtttt
     2041 agcaattttt actccatttg gttgaatatg tggtgtacct tttgtcattt gttatcttct
     2101 ccttaatatt cattaataat tttaagataa catttgtttc ccttttttgc gaatttttgt
     2161 actatagaat taaagaatta taaaaatata atcctgatta gcttgttgtc aagtcatcgt
     2221 tcataatgaa gatatcacgt tcaattgtat tgtttgttta tgggaaatga attaatgtaa
     2281 tagtatatgt atgcggttac ataaaaagcg aacatctaac ctgatattta aatgaacctg
     2341 acgctcaatc aactaattta caaccgtatt tttataatca accataaagg aggagataga
     2401 aaatgaatag tgcaaaattg attgatcaca ctttattgaa gcctgagtca acacgtacgc
     2461 aaatcgatca aatcatcgat gaagcgaaag cataccattt taaatctgta tgtgtgaatc
     2521 caacgcatgt taaatatgca gcagagcgac tagctgattc agaggtgtta gtttgtacgg
     2581 taataggatt cccattaggt gcatcgacaa ctgcgacgaa agcatttgaa acagaagatg
     2641 cgattcaaaa tggtgcagat gaaattgaca tggtcatcaa catcggcgca ttaaaagatg
     2701 gacgttttga tgatgtacaa caagacattg aagcagtggt gaaagctgcg aaaggtcaca
     2761 cagtaaaagt gattattgag acggtattgt tggaccatga cgaaattgta aaagcgagtg
     2821 aattaacaaa agcggctggt gcggacttcg ttaaaacttc aacaggtttt gcaggtggcg
     2881 gtgcgactgc agaagacgtt aaattaatga aagatacagt aggtgctgat atagaagtaa
     2941 aagcatcagg tggcgtacgt aatttagaag atttcaataa aatggttgaa gcaggtgcga
     3001 cacgtattgg tgcgagcgca ggcgttcaaa ttatgcaagg tttagaagca gattcagatt
     3061 actaatttta taaaaatatg taagcatggg acatcgtaac catatataag tatgtcgata
     3121 tcagctttaa gacatcataa atgccatgat gattctaaga taatttaata gggactttaa
     3181 cgttataacc aatctattta agaataataa catcgcattg aaattgtgat attaaaaatg
     3241 aaatcgatag cttgctataa taattttcgc ttgtgctttt aaaaatggac tagagaaagc
     3301 gcagatgctt tgtcctaagc tttttaagaa aaggatgata aaaatgagaa tgatagacat
     3361 tattgagaaa aagcgtgacg gtcatacact aacaacggaa gaaattaatt tctttattgg
     3421 cggctatgtt aaaggggata ttcctgatta ccaagcatca agtttagcaa tggcgattta
     3481 tttccaagat atgaatgatg atgagcgtgc agcattaacg atggctatgg ttaattctgg
     3541 tgatatgata gatttgagtg atattaaagg tgtcaaagta gataagcact caacaggtgg
     3601 tgtaggagat acaactacat tggttttagc accattagta gcagctgtag atgttcctgt
     3661 tgcaaaaatg agtgggcgtg gattaggtca tacaggtggt acgattgata aattagaagc
     3721 aattgatggt tttcatgttg aaatagatga agcaacattt gtgaaattgg ttaatgaaaa
     3781 taaagtggca gttgtaggac aatcaggaaa tttaactcct gcagacaaaa aattatatgc
     3841 cttaagggat gttactggta ctgtcaattc aataccatta attgcctctt caattatgag
     3901 taaaaagatt gctgctggtg cagatgcaat tgtattagat gtaaaaactg gtagcggtgc
     3961 atttatgaaa acattagaag atgctgaagc attagcgcac gcaatggtac gtattggtaa
     4021 taatgtggga cgtaatacga tggcgattat ttctgatatg aatcagccac ttggacgtgc
     4081 gattggcaat gcgcttgagt tacaagaagc aattgatacg ttgaaaggac aaggtccgaa
     4141 agatttaaca gaacttgtat taacattagg ttctcaaatg gttgtacttg caaacaaagc
     4201 tgaaacgctt gaagaagcga gagcgttgct aattgaggca attaattcag gtgcagcatt
     4261 agaaaaattc aaaacattta ttaaaaacca aggtggcgac gaaactgtta ttgaccatcc
     4321 agagcgtttg ccacaagctc aatatcaaat tgaatataaa gctaaaaaat caggttatgt
     4381 gactgaatta gtctctaacg atataggtgt cgcttcgatg atgttaggag cgggacgttt
     4441 aacaaaagag gatgatattg atttagcggt tggtattgtt ttaaataaaa aaattggtga
     4501 taaagtagaa gaaggagaat cattattaac gattcacagt aatcgccaag atgtagacga
     4561 tgttgttaaa aaattagatt caagcataac aattgctgat catgttgtat cgcctacatt
     4621 aattcataaa atcattacag aataggagtt gaagctgatt gaacatacta agatatttat
     4681 taggaatagg ttttagtgta ataggtgtat tgcattttac acgagaacga caatttagaa
     4741 atatcatacc gaaatgtttg ccacttcgaa aaacagcggt tcttgttaca ggaatctttg
     4801 agatattatt tggacttgca cttttaatta aaagaccatc tcaatgcttg aaaaatacaa
     4861 tcaatctatt cttgctagct gtattcccag ctaatatcta tatggcagtt aaaaagttac
     4921 ctttaggaga taaacaattg ccaacatggc aactatattt aaggttacct atgcagttcg
     4981 tgttaatggg actaattaag aaattatagt ggttctcaaa taaatcggac aaatgttaaa
     5041 gattttactt tttcatttgt tcgatttttt gactttgaag cataataaaa agcagttgga
     5101 caatggcgat tgtaaaatca ttgttcgact gcttttctga ttaacttgat tgactaagat
     5161 ttattttcct gtaccgaaaa catcatgcca ttcgtttcgt ttgtctagaa atgcttgagc
     5221 gatagtttta gcaccttcta atgaatgact tgctgcccag ccacattgta cttcattaca
     5281 agcaggcact tcagtagcat ttagcacatc atttaaagtt gcttcaacaa tatttaatac
     5341 atcatcataa ttatcatgat taataaatga tacatagaaa ccagtttggc aacccatagg
     5401 acttaaatca acaactttgt cactatgatt tctaatattt tcagccatta aatgttctaa
     5461 tgaatgtagt ccgggcatat ccatatgttc tttgtttggt tgtttgaaac gaatgtcgta
     5521 tttgtgaatg acatctccgt ttaatccttc catcgttccc gctaatctaa taaatggggc
     5581 aaccacctta gtatgatcta aattgaaact ttcaacattc atttttgtca ttttgaattt
     5641 cctcctatta actactcaaa actataaaac gcttatgatt tgttgcatta taaaacttaa
     5701 gtttgttgta agttgtgttt ctaacaatac ttaaattgta acaatagggc ttatgtttta
     5761 caatttgaaa ctgttttata aatttatgaa aggcttttag ttattagaaa attttgtaaa
     5821 taaagaaaaa ctagatgaca tcatgagata aaaccgatag aataagtaat aattcatttt
     5881 gaaagaacaa aatattgtgg atgattaaaa tgagtgtagg gtaaaaggga gatagtaaaa
     5941 aatgagtgaa aaacaacaaa ttctcgatta tatagaaaca aataaatata gttatatcga
     6001 aatcagtcat agaattcatg aacgtcctga acttggtaat gaagaaatat ttgcgtctcg
     6061 aactttaata gatcgtttga aagagcatga ttttgaaatt gaaaccgaga ttgctgggca
     6121 tgcaactggg tttatagcga catatgattc aggacttgac gggccagcta taggtttttt
     6181 agcagaatac gatgctttgc caggattggg tcatgcttgt ggtcataata tcattggaac
     6241 tgcaagtgtt cttggtgcaa ttggtttgaa gcaagtgatt gaccaaattg gtggtaaagt
     6301 agtcgttctt ggatgtccag ctgaagaagg tggggaaaat ggtagcgcta aagcttctta
     6361 tgtcaaggct ggtgtgattg atcaaataga cattgcctta atgattcatc cgggaaatga
     6421 aacttataaa acgattgata ctttggcagt ggatgtttta gatgttaaat tttacggaaa
     6481 aagtgctcat gcctctgaaa atgcagatga agcgttaaat gcattagacg ctatgattag
     6541 ttattttaat ggtgtagcac aactacgaca acatattaaa aaagatcaac gtgtgcatgg
     6601 tgtgatttta gatggcggga aagcagctaa tattattcca gactatacac atgctcgttt
     6661 ttatactaga gcaatgacgc gtaaagaatt ggatatatta acagaaaaag taaatcaaat
     6721 cgcacgtgga gctgcgatac agactggttg tgattatgaa tttggtcgaa ttcaaaacgg
     6781 tgtgaatgaa ttcattaaaa cgccgaaatt agatgattta tttgctaaat atgctgaaga
     6841 agttggtgaa gcagttattg atgatgattt tggttatggc tctacggata cagggaacgt
     6901 aagtcatgtt gtgccaacaa tacatcctca tattaaaata ggatcacgta atttagtagg
     6961 acatacgcat agatttagag aagcggctgc gagtgtacat ggtgatgaag cattaattaa
     7021 aggcgctaaa ataatggcgt tgatggggtt agagttaatt acaaatcaag acgtttatca
     7081 agacattatt gaagagcatg cgcatttgaa agggaatggg aagtaatgac aaataatggt
     7141 aatgagccga acttaacgtt aagtgattta tatgacaaag atgttgtcta tacgtcaaga
     7201 ccttcgtata tatcgaatcc atggttgaaa ccggacgaac atcaatctaa ttttttaact
     7261 ggtagggagt tattaatagc aaatcaatta cctgttattg ttcacgaagc tagtgcgaca
     7321 gacaaattgc atcagctatt tcaagttatt ggtaaggaag taccgaactc aatttataca
     7381 tttaacaatc agcaatctta cgaaaacttg atcaaacaat tggcacataa ggaaaataaa
     7441 aagatttatt ttcaatatat tcatgatgaa acaatcttga atcagcaata ttatgcatta
     7501 gataaaacgt tatttgttgc tttaaataat aaagcaagaa tacctgaatg gacaaatggg
     7561 aagtttttac caaaaagaaa agttgtaaaa attgagcaat tcgaaaacga gattaagaat
     7621 tgggaattcc cgttagtaat taagccggga gatgatcttc cgacagcagg tggctatggc
     7681 gttatgattt gttatcatga tgcagattta caaaaagcga taacgcgtat taaagaggca
     7741 actgctgaaa cgaattcgct tattatcgaa cagaagattg aagaaaaagc gaattattgc
     7801 gttcagtttg catactcgga atcgctaggc attcaatatc ttggtgcggc aacgcaatta
     7861 acggataagt acggatttta taacggaaat gaaaatacta ctaatgtacc agagcacgtt
     7921 atagaagcag gtagacaaat tatggaaaac ggtgtgaatc aaggattctt tggtgtagct
     7981 ggttttgacc tactcgtcga tgaggatgat aacgtttatg cgattgattt aaactttaga
     8041 caaaatggtt caacgagcat gttattactt gctaacgagt tgaattcagg atatcaaaag
     8101 ttttatagtt atcattcaaa aggtgataac acatatttct tcaatacgat tttgaaatat
     8161 gtcaaagaag gtagtttata cccgttatct tattatgatg gtgattggta cggtgaagat
     8221 aaagttaaat caaggtttgg ctgtatttgg catggtgatt caaaagaaac agtactggag
     8281 aatgaacgcg catttttagc tgaacttgaa cactattaga gttcggaaca taaggcgcta
     8341 caatgttgtg ttgccagtag ttgactgaat atgcgtttgt aacaagcttt tttcgattct
     8401 agtcaacagt aattaaattt atgatatggc aatactttgt aatactaata ttaaatggcg
     8461 acttttattt cactatgtta taagagttgc cattttgttg ataaaggtat actaaaggtt
     8521 atcgttttga aatttttagt aactagatat gtttcgtgct atagaccgaa tttgtgtata
     8581 cgtaaaattt aatgctattg aatttttaaa atgaaaaaca tgacattaaa ttgaattcat
     8641 aatatgtcta attgactaac ttgttggagt catttactat tttatgtatg acatatttta
     8701 aaaagtgagg gtcaagcatg tcttataaag catatccatt ctttagagat atattaataa
     8761 atgaatgtat ttatttcgcc tctaaaaata aaaaactagt acgcctaaat tataaaagtg
     8821 aagcgtatgt aggcgtttgg acagaagaaa gtgtggccgt atcattttta acaagtcgtg
     8881 atattccatt tgataaagtt gtaaaaatgg acgttgatcg ttttgctact tatgaattag
     8941 atgaattgtt tgatgaacaa gaccatatta ttatgaatca aacaatggaa gaagaagggc
     9001 atctactaaa cgttgtagct gttacacaag aagtgatgac ggaattagat aaaattagaa
     9061 tcaaagaatt tgtccaagat gtagcgaaat atgatgaagt atacggctta actaaaaaag
     9121 gtagtaagca gtttattctc attagtgaaa atgatagcga cgaaaaaaag ccgcatatta
     9181 tgcctgtatg gagtattaaa aacagagcgt taaaagttcg agatgaagat tttgaagagt
     9241 gtgatttaat tacgattgaa ggttctgttt tcggagaatg gctagatgaa cttagagatg
     9301 atcataaagc cgttgcgata gatttaaaaa ctggcgtggt tggtacaatt gtttcagcgc
     9361 aaaaattgtc taatgaactt acattttaat tgaattgagt tgtaagtaat taagtgataa
     9421 taaatggaac aatacgtatt caaaacactt agaccataaa ataaaaggcc atttatatag
     9481 cgtttattta aaacaacgcg catataaatg gtctttttct atttttctaa atataatgca
     9541 ccaatagcac ctgaaaatgc gccgttttca acatagtacg gtttgcaacc gcgtaacaca
     9601 gtataatctt ccacaacttt gcgtaataaa gcgttattat gaaatgaaga accgatataa
     9661 acgatatttt cagttttaaa ttcacgtgca acagtaatgg ccattgtcgt aacaacttcg
     9721 ccaacgacac caataacggc tgctaattta ttgctaggtg taaaatcagc atctaaatga
     9781 tgtagtacat gaccaaaatt agctgctgtt aaatcaccgg gaatgggtgg ttcggtatct
     9841 ttataaatat gtctaacctt taaatcgata gtgttacgat caccgtgttg tgccatgtca
     9901 gttaactgtt tataatcagt gatttgactt agtaaataac cgagtccttg aatcatgcct
     9961 ccacctgtac cgataccgcc tacacgacgt tgtgattggc cgtcgaaata atgtagtgac
    10021 gtaccggtac caacatttgc aaaaatataa tctgctaagt catggccttg ctcttttaac
    10081 aaaataccta gtccttgaga tgcagcatca aactctacaa aaatttgtgc aggaatgttg
    10141 atgttttcag caatgacacc tgcattacct ccagttaagc ataatttttc aatttgctgt
    10201 tggtttaacc attccacaac ttgatcaata tttttagtta attcagtttt aaaagtacgt
    10261 tggttatctt gctcttgaac gattttaatt agtgtaccgc cagcgtcaat gccaactttc
    10321 ataagattcc cacctcatta ttaatgtcta tccttaaata atagtatagt aaaatgacta
    10381 aaaaacaagt aataatagta attattaaca aatttgatgc cattgcattt caacattgta
    10441 agcgtatcgc aattaatgtt ttacaaacgt ggacgttaag ttatatatat tattttctag
    10501 gaattttgaa gttgtatagg attgttagtt agtgacgcaa tattaaaagt agttcgtacg
    10561 cagtgtattt gtaagtctct gattaaaatg ataa
//
LOCUS       DAXWFK010000090        10560 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.090, whole genome shotgun sequence.
ACCESSION   DAXWFK010000090 DAXWFK010000000
VERSION     DAXWFK010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10560
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.090"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(37..843)
                     /gene="yjbH"
                     /locus_tag="ACGEXS_001719"
     CDS             complement(37..843)
                     /gene="yjbH"
                     /locus_tag="ACGEXS_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease adaptor protein YjbH"
                     /protein_id="HFF8012720.1"
                     /translation="MAGELRIMENKSREDINLSPVSKIEIYSFFDPFSSDCFKLSAIL
                     SKLRIEYNQYIRIRHILNPSLKVLTKCQAQSTSNFDNIALAYKAAELQGRVRAERFIH
                     LMQNEIIPKRDIITESMICDCIQNAGIDLEVFKDDLQKSKLTESLKIDLHIAREMEIE
                     QAPSLVFFSEDVHEEGLKVEGLYPYHIYTYIINELMGKPIEKNLPPKLETYIQQQQLV
                     TMEELLTIYEWPEKLLNKELKKLAIQQKIEKLKYPDGDFWKSKMPKIKSK"
     gene            complement(866..1231)
                     /locus_tag="ACGEXS_001720"
     CDS             complement(866..1231)
                     /locus_tag="ACGEXS_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="truncated hemoglobin YjbI"
                     /protein_id="HFF8012721.1"
                     /translation="MTTTPYDIIGKEALYDMIDYFYTLVEKDERLNHLFPGDFAETSR
                     KQKQFLTQFLGGPNIYTEEHGHPMLRKRHIDFTITEFERDAWLENMQTAINRAAFPQG
                     VGDYLFERLRLTANHMVNS"
     gene            complement(1335..1928)
                     /locus_tag="ACGEXS_001721"
     CDS             complement(1335..1928)
                     /locus_tag="ACGEXS_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFF8012722.1"
                     /translation="MATNHEIEFKQMITASIYNKLQEKYFKDSVLFKQVNYYIDTPDF
                     KLKEHRSALRIRVKDNQYEMTLKTPAKVGLLEYNYIVDIKPEMNLIISNDNLPDDIRQ
                     IIVEQFGVKDTTLSILGALTTYRQETKYKGDLLVLDKSEYFDTTDYELEFEVKDYNQG
                     LQKFQSLLNELNLEHHQPLNKVQRFFKKKETLSNNIN"
     gene            2114..2461
                     /locus_tag="ACGEXS_001722"
     CDS             2114..2461
                     /locus_tag="ACGEXS_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012723.1"
                     /translation="MRLYINEIKIKDDILYCYTEDSIKGLSEVGQMLVDSDNYAFAYT
                     LDDGKAYAYLIFVQETWTMLHEKMTKKIIINDELELTEFHQELTYILDNIKGNNNYGK
                     EFVATVEETFDIE"
     gene            2478..3113
                     /locus_tag="ACGEXS_001723"
     CDS             2478..3113
                     /locus_tag="ACGEXS_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFF8012724.1"
                     /translation="MNQWDQFLTPYKQAVDELKVKLKGMRKQYEVGEQASPIEFVTGR
                     VKPIASIIDKANKRQIPFDRLREEMYDIAGLRMMCQFVEDIDVVVNILRQRKDFKVIE
                     ERDYIRNTKESGYRSYHVIIEYPIETLQGQKFILAEIQIRTLAMNFWATIEHTLRYKY
                     DGAYPDEIQHRLERAAEAAYLLDEEMSEIKDEIQEAQKYYTQKRSKKHEND"
     gene            3130..3939
                     /locus_tag="ACGEXS_001724"
     CDS             3130..3939
                     /locus_tag="ACGEXS_001724"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFF8012725.1"
                     /translation="MRYTILTKGDSKSNALKHKMMNYMKDFRMIEDSENPEIVISVGG
                     DGTLLQAFHQYSHMLSKVAFVGVHTGHLGFYADWLPHEVEKLIIEINNSEFQVIEYPL
                     LEIIMRYNDNGYETRYLALNEATMKTENGSTLVVDVNLRGKHFERFRGDGLCVSTPSG
                     STAYNKALGGALIHPSLEAMQITEIASINNRVFRTVGSPLVLPKHHTCLISPVNHDTI
                     RMTIDHVSIKHKNVNSIQYRVANEKVRFARFRPFPFWKRVHDSFISSDEER"
     gene            3936..4790
                     /locus_tag="ACGEXS_001725"
     CDS             3936..4790
                     /locus_tag="ACGEXS_001725"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFF8012726.1"
                     /translation="MKFKYHISQQETVKTFLARHDFSKKTVSAIKNNGALIVNDEPVT
                     VRKQLMTNDILEIHLPREIPSVNLIPYARKLEVLYEDAFIIIVTKPNNQNCTPSREHP
                     HESLIEQVLYHCQEHGENINPHIVTRLDRNTTGIVIFAKYGHIHHLFSKVNLKKIYTC
                     LVYGKTHTSGIIEANIRRSKDRIITREVASDGKYAKTSYEVINQNDKYSLCKVHLHTG
                     RTHQIRVHFQHIGHPIVGDSLYDGFHDKIHGQVLQCTQIYFVHPINKNNIYITIDYKQ
                     LLKLFNQL"
     gene            4811..6196
                     /gene="mgtE"
                     /locus_tag="ACGEXS_001726"
     CDS             4811..6196
                     /gene="mgtE"
                     /locus_tag="ACGEXS_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFF8012727.1"
                     /translation="MSMNTDEKERVQEELYDQTLLDQYLENDDIDQFRDEFLALHTYE
                     QSEYFEDTTDENRQKIFQYLSPEEVANFFDQLDIDDDEYELLFDKMNATYASHILEEM
                     SYDNAVDILNELTKPKVASLLTLMNKDDANEIKALLHYDEDTAGGIMTTEYLSLKAHT
                     PVKEALLLVKAQAPDAETIYVIFVVDDDGKLVGVLSLRDLIVAENDAYIEDIMNERVI
                     SVNVADDQEDVAQVMRDYDFMAVPVIDYQEHLLGIITIDDILDVMDEEASEDYSRLAG
                     VSDIDSTNDSIIKTALKRLPWLIILTFLGMITATILGRFEKTLENVALLAAFIPIISG
                     MSGNSGTQSLAVSVRNITTGEINEQSKFRIALREAGSGVLSGVVCSTILFTIIVAIYH
                     QPLLALIVAGSLTCAMTVGTFVGSMIPLLMNKLNIDPAVASGPFITTINDIISMLIYF
                     GLATSFMAYLI"
     gene            6206..8050
                     /locus_tag="ACGEXS_001727"
     CDS             6206..8050
                     /locus_tag="ACGEXS_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFF8012728.1"
                     /translation="MEFLSLVIVVLAAFLTPIIVNRLNINFLPVVVAEILMGIVIGNS
                     FLNIVERDSILNILSTLGFIFLMFLSGLEIDFKAFKKDKRARQGQNDDESSIPGHLNL
                     ALTVFAFIMIISILLAYVFKWLGLVDDVLLMVIIISTISLGVVVPTLKEMNIMRTTIG
                     QFILLVAVLADLVTMILLTVYGAINGQGGSTIWLIGILVVFTAISYILGVQFKRMSFL
                     QKLMDGTTQIGIRAVFALIILLVALAEGVGAENILGAFLAGVVVSLLNPDEEMVEKLD
                     SFGYGFFIPIFFIMVGVDLNIPSLIKEPKLLIIIPILIVAFIISKLIPVMFIRRWFDM
                     KTTIASVFLLTSTLSLVIAAAKISERLNAISAETSGILILSAVITCVFVPIIFKKLFP
                     VPDEFNRKIEVSLIGKNQLTIPIAQNLTSQLYDVTLYYRKDLSDRRQLSDDITMIEIA
                     DYEQDVLERLGLFDRDIVVCATNDDDINRKVAKLAKAHQVERVICRLESTTDDTELVD
                     SGIEIFSSYLSNKILLKGLIETPNMLNLLSNVETSLYEIQMLNYKYENIQLRNFPFGG
                     DIIFVRIIRNNESIVPHGDTQLRYGDRLIVTGAKEYVDELKQELEFYF"
     gene            8328..9098
                     /gene="fabI"
                     /locus_tag="ACGEXS_001728"
     CDS             8328..9098
                     /gene="fabI"
                     /locus_tag="ACGEXS_001728"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFF8012729.1"
                     /translation="MLNLENKTYVIMGIANKRSIAFGVAKVLDQLGAKLVFTYRKERS
                     RKELEKLLEQLNQPEAHLYQIDVQSDEEVINGFEQIGKDVGNIDGVYHSIAFANMEDL
                     RGRFSETSREGFLLAQDISSYSLTIVAHEAKKLMPEGGSIVATTYLGGEFAVQNYNVM
                     GVAKASLEANVKYLALDLGPDNIRVNAISAGPIRTLSAKGVGGFNTILKEIEERAPLK
                     RNVDQVEVGKTAAYLLSDLSSGVTGENIHVDSGFHAIK"
     gene            complement(9293..10378)
                     /gene="cozEa"
                     /locus_tag="ACGEXS_001729"
     CDS             complement(9293..10378)
                     /gene="cozEa"
                     /locus_tag="ACGEXS_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell elongation protein CozEa"
                     /protein_id="HFF8012730.1"
                     /translation="MLNKVWFRTGIALIMLFILIKLFMEVHEVFTPIATIIGSVFLPF
                     LISGFLFYICLPFQNLLEKVGFPRWASITTIMLALFAIIGLIVAFVAPIIISNINNLI
                     SQTPALQKEAEQIIKFALAQMDKLPEDVTSRITNMVKSMGDGVTNILSNSLQYITSLI
                     STIFLLIMVPFFLIYMLKDHEKFIPAVAKFFKGERKVFFVDLLTDLNFTLKSYIQGQV
                     TVSVILGIFLYIGYSIIDLPYIPLLVLFAGVANLIPFLGSWLSFAPAAILGIIDSPTT
                     FIWVCIITLIAHQLEGNIITPNVMGKSLSIHPLTIIVVILAAGDLGGFTLVLIAVPLY
                     AVLKTVVSNIFKYRQRIIDKANSNVKD"
BASE COUNT         3611 a         1481 c         1864 g         3604 t
ORIGIN      
        1 tcgttacatt tatggtgtac gaggtcttta atttatttat tttgatttga ttttaggcat
       61 tttagatttc cagaaatcgc catctggata tttaagtttt tcaatttttt gttgaatggc
      121 taacttcttt aactctttgt ttaaaagttt ttctggccat tcataaatag taagtaattc
      181 ttccatcgtt acaagttgtt gttgctgtat ataagtttct aatttaggag gaagattctt
      241 ttcgataggt ttacccatca attcattaat tatataagta tagatgtgat atgggtataa
      301 tccttcgact tttaaacctt cttcatgaac atcttcacta aagaaaacga gagaaggggc
      361 ttgttcgatt tccatttctc ttgcaatatg caaatcaatt ttcaagcttt cggttagttt
      421 acttttttgt aagtcgtctt taaatacttc taaatcaata cctgcatttt gaatacagtc
      481 acaaatcatt gattctgtaa taatatctct tttaggtatg atttcatttt gcattaaatg
      541 tataaatcgt tcggcacgta cacgaccttg taactcagct gctttataag ctagggcgat
      601 gttatcaaag ttggatgtac tttgagcttg gcatttcgtt aatactttta acgaaggatt
      661 taatatatgt ctgatacgta tatattgatt atattcaatt cttaatttgg ataagattgc
      721 tgataatttg aagcaatcgg agctaaatgg atcgaaaaat gaataaattt cgattttact
      781 tacaggtgat agattgatat cttcacgact cttattttcc attattcgta attctccagc
      841 catgtttatt cacctacaat taattttagg aattcaccat atgattagca gttaatctta
      901 agcgctcaaa taaataatct ccaacacctt gtggaaacgc agcgcgatta attgctgtct
      961 gcatattttc taaccatgca tctctttcaa attcagtgat tgtaaaatct atatgtcttt
     1021 ttcttagcat aggatgtccg tgttcttcgg tataaatgtt tggaccgccc aaaaactgtg
     1081 ttaaaaattg tttttgttta cgacttgttt ctgcaaaatc tcctggaaac aggtgattaa
     1141 gtcgttcatc tttttctaca agggtgtaaa aataatcaat catatcgtat aacgcttctt
     1201 taccaatgat gtcatatggt gttgttgtca tttaatcacc cattttcaaa aatttactgt
     1261 tacgaactta agttaatata taactaatat aacatgattt taaacatttg aaagaaatat
     1321 gcatatttgc caatttaatt tatattgttt gaaagtgttt cttttttctt gaaaaaacgt
     1381 tgaactttat ttaaaggttg atgatgttcg aggtttagtt cgtttaataa agattggaac
     1441 ttttgtaaac cttgattata gtctttaact tcgaactcta actcataatc cgtagtatcg
     1501 aaatactcac ttttatctaa aaccagtaaa tcacctttat atttagtttc ttggcgatat
     1561 gtcgttaatg caccaagtat tgataaagtt gtatctttta caccaaactg ttcaactata
     1621 atttgacgaa tgtcatctgg aagattgtcg tttgaaataa tcaagttcat ctctggttta
     1681 atgtcgacga tatagttgta ttctaataga ccaacctttg ctggtgtctt taaagtcatt
     1741 tcatattgat tgtctttaac tcttatgcgt agtgcagagc gatgttcctt taatttgaaa
     1801 tcgggtgtat caatatagta attgacttgc ttaaaaagca cactgtcttt aaaatatttc
     1861 tcttgcaatt tattatagat tgatgcagtt atcatttgtt taaattctat ttcatgattt
     1921 gttgccatga tatgtataca cctcgtatca aattcaattt atcttaacta tattatgaat
     1981 gacaaagttg aattttaaaa gtaatttcct ttatctatta tcaatgttaa tttgaccatt
     2041 aaaaatagtg ttcgtaagtg ttttgtatta ttgaattgtg ttaaaatgtt atggaataag
     2101 aggaggatta agcatgcgtt tatatattaa tgaaattaaa attaaagatg acatacttta
     2161 ttgttataca gaagattcta ttaaaggatt atctgaagta ggacaaatgc tcgttgatag
     2221 tgataattat gcctttgcgt atacattaga tgatggtaaa gcgtatgctt atctcatttt
     2281 cgtacaagaa acatggacga tgttgcatga aaagatgact aaaaaaatta ttatcaatga
     2341 tgaactagaa ttgactgaat tccaccaaga acttacttat attttagaca acataaaagg
     2401 gaataataat tatggtaagg aatttgttgc aaccgttgaa gaaacattcg acattgaata
     2461 aagcggggtg aagcactatg aatcaatggg atcagttctt aacaccttat aagcaagcgg
     2521 ttgatgagtt gaaagtgaaa cttaaaggca tgcgcaaaca atatgaagtt ggtgaacaag
     2581 cgtcgccaat agaatttgtt actggtcgtg ttaaaccaat cgctagtatt atagataagg
     2641 caaacaaacg acaaatacca tttgataggt taagagaaga aatgtacgat atcgctggtt
     2701 taagaatgat gtgccaattt gttgaagata ttgatgttgt cgtcaatatt ttaagacaaa
     2761 gaaaagattt taaagtaatt gaagaacgag attatattcg taacactaaa gaaagtggtt
     2821 accgctcgta tcatgtcatt attgaatatc caattgaaac attacaaggc caaaaattta
     2881 tattggctga gattcagatt cgtacattag caatgaattt ctgggcaacg attgaacata
     2941 ctttacgata taaatatgat ggtgcttatc cggatgaaat tcaacatcgt ttggaaagag
     3001 cggcagaagc agcgtattta cttgatgaag agatgtctga aattaaagat gaaattcagg
     3061 aagctcaaaa atattacacg caaaaacgtt ctaaaaaaca tgaaaatgat taacgaggtg
     3121 ttataaatca tgcgttatac aattttaact aaaggtgact ccaagtctaa tgccttaaag
     3181 cataaaatga tgaactatat gaaagatttt cgcatgattg aggatagtga aaatcctgaa
     3241 attgttattt cagttggtgg tgacggtaca ttactacaag cattccatca gtatagccac
     3301 atgttatcaa aagtggcatt tgttggagtt catacaggtc atttaggatt ttatgcggat
     3361 tggttacctc atgaagttga aaaattaatc atcgaaatta ataattcaga gtttcaggtc
     3421 attgaatatc cattgcttga aattattatg agatacaacg acaacggcta tgaaacaagg
     3481 tatttagcat taaatgaagc aacgatgaaa actgaaaatg gctcaacact tgttgtggat
     3541 gttaacttaa gagggaaaca ctttgagcga tttagaggcg atggattatg tgtatcaaca
     3601 ccttcgggtt caacggctta taacaaagcg ctaggtggcg cactgataca tccttcactt
     3661 gaagcaatgc aaattacaga aattgcctcg ataaataatc gtgtgtttag aacggtagga
     3721 tcaccacttg tattaccaaa gcatcataca tgtttaatat caccagttaa tcatgatacc
     3781 attagaatga cgatagatca tgttagtatc aaacataaaa atgttaattc aatacaatac
     3841 cgtgtagcaa atgaaaaagt gaggtttgca cgttttagac cattcccatt ctggaaacgt
     3901 gtgcacgatt ctttcatatc aagtgatgaa gaacgatgaa atttaagtat catatatcac
     3961 aacaagaaac tgttaaaact tttttagcac gacatgattt ttctaagaag acagtgagcg
     4021 ccattaaaaa taatggcgct ttaattgtta atgatgaacc agtcacagtg cgtaagcaat
     4081 taatgacaaa tgatatatta gaaattcatt taccgcgaga aataccgagt gttaatttaa
     4141 taccttatgc tcgtaagcta gaagtattgt atgaagatgc ttttatcatc atagttacta
     4201 aaccaaacaa tcaaaattgt acgccttcga gagaacatcc tcatgaaagt ttaatcgaac
     4261 aagtactata tcattgtcag gaacatggtg aaaatattaa cccacatatt gttacgcgtc
     4321 tagatcgtaa tacaactggt attgtgatat tcgctaaata tggacatatc catcatttat
     4381 tttctaaagt aaacttgaaa aaaatatata cttgccttgt atatggtaaa acccatacat
     4441 ctggtattat tgaagctaat attagacggt caaaggatag gattataact agagaagttg
     4501 cctcggatgg taaatacgct aaaacatctt atgaagtaat aaatcagaat gataaataca
     4561 gtttatgcaa agttcatttg catacgggac gtacacatca aattcgtgta cattttcaac
     4621 atattgggca tccaattgtg ggagattctt tgtatgatgg ttttcatgac aaaattcatg
     4681 gtcaagtact gcaatgtacg caaatatatt ttgttcatcc aatcaataag aacaatattt
     4741 atattacaat tgattataag caattactta aattattcaa tcaactctaa ttcacacagg
     4801 gggtgtaagt atgtcaatga acacagatga aaaagagcgt gttcaagagg aattatacga
     4861 ccaaacttta ttagatcaat atttagaaaa tgatgatatt gatcaattta gagatgaatt
     4921 tctagcatta cacacatatg aacaaagtga gtattttgaa gatactaccg atgaaaatag
     4981 acaaaagatt tttcaatatt tatcacctga agaagttgca aatttctttg atcaattaga
     5041 tattgatgac gatgaatatg agttgctatt tgataagatg aatgcgacat acgcaagtca
     5101 catattagaa gaaatgtcat acgacaatgc agtagatatt ttaaatgagt tgactaaacc
     5161 aaaagttgct agtcttttaa cattgatgaa taaagatgac gcgaatgaaa tcaaagcatt
     5221 acttcactat gatgaggata cggccggcgg tattatgacg acggagtatt tatcacttaa
     5281 agcgcatacg cctgttaaag aagcattatt attggtcaaa gcgcaagcac cagacgcaga
     5341 aacaatatat gttatatttg tcgttgatga tgatggtaaa ttagtaggtg ttttatcgct
     5401 aagagattta attgtagctg aaaatgatgc ttatattgaa gatattatga atgaacgtgt
     5461 cattagtgtg aatgtagcag acgaccaaga agatgttgct caagttatga gagactatga
     5521 tttcatggct gtacctgtta tagattacca agaacatttg cttggtatca tcacgattga
     5581 tgatatttta gacgttatgg atgaagaggc tagtgaagac tactctcgtt tagccggggt
     5641 atcagatatc gattcgacta atgattcaat cattaaaaca gcattaaaac gtttaccatg
     5701 gttgattatt ttaacatttt taggaatgat tactgcgaca attttaggga gattcgaaaa
     5761 aacattagaa aatgtagcgc tactcgcagc gtttattcct attattagtg gtatgtcagg
     5821 aaattcaggt acacaatctt tagccgtttc agttcgtaac attacgacag gggaaattaa
     5881 tgagcaaagt aaatttagaa ttgcattaag agaagcagga agtggtgtat tatcgggtgt
     5941 tgtatgttca acaatattat ttacaattat tgttgcaata tatcatcagc cacttttagc
     6001 attaatcgtt gcaggaagtt taacttgtgc gatgacggtg gggacgtttg taggttcgat
     6061 gattccatta ttgatgaata aattaaatat cgatccagca gtggctagtg gaccatttat
     6121 tacaacaatt aatgatatta ttagtatgtt gatttatttt ggtttagcta catcatttat
     6181 ggcttactta atttaaggag gagttatgga gtttttatct ttagttattg ttgttttagc
     6241 agcgttttta actccaataa ttgtcaatcg attaaatatt aatttcttgc cagttgttgt
     6301 tgcagaaatt ttgatgggga ttgtgattgg aaattcattt ctaaatatag tagaaaggga
     6361 ttcaattcta aatattttat caacgttagg ctttatcttt ttaatgtttt taagtggttt
     6421 agaaattgat tttaaagctt ttaaaaaaga taaacgcgca cgtcaaggac aaaatgatga
     6481 tgaatcctca attccagggc atcttaatct agcgttaact gtatttgcat ttattatgat
     6541 tatttcgatt cttttagcgt atgtatttaa atggcttgga ttagtggatg atgtgttatt
     6601 aatggtcatt atcatttcaa ctatttcctt aggcgtagtt gttccaactt taaaagaaat
     6661 gaatattatg agaacaacta tagggcaatt tatcctatta gtagcagtac ttgcggactt
     6721 agtaactatg attttattaa cggtctatgg cgcaatcaat ggtcaaggcg gcagtacaat
     6781 atggttaata ggtatattag ttgttttcac agcaatttca tatattttag gtgttcaatt
     6841 taaaagaatg tcatttttac aaaaattgat ggatggtacg acgcaaatcg gtattcgtgc
     6901 ggtatttgca ttaataatat tattagtagc cctagcagag ggagttggcg cagaaaatat
     6961 attaggtgca ttcttagcag gtgtcgttgt ttcattatta aatccagatg aagaaatggt
     7021 tgaaaagtta gactcatttg gttatgggtt ctttattcct attttcttta taatggttgg
     7081 tgtagattta aacatacctt cattaattaa agaaccgaaa ttactaatta tcataccgat
     7141 tttaatcgtt gcatttatca tttcaaaatt aattccagtc atgtttattc gacgttggtt
     7201 tgatatgaaa acaacgattg catcagtatt tttattaaca tcaacattat cgctcgtgat
     7261 agctgcagcc aaaatttcag aaagattaaa tgctatttca gctgaaacgt caggtatatt
     7321 aattttaagc gcagtcatta catgtgtatt cgttccgatt attttcaaaa aactgtttcc
     7381 agttccagat gagtttaacc gtaaaattga agttagttta attggtaaaa atcaattaac
     7441 gattcctata gcgcaaaatt taacatctca gttatatgac gtgacattat attatcgcaa
     7501 agacttgagt gatcgtcgtc aattgtcaga tgatatcacg atgatagaaa ttgctgatta
     7561 tgaacaagat gttttagaac gactaggtct gtttgaccga gacatagttg tttgtgctac
     7621 gaatgacgat gatattaacc gaaaagttgc taaattagcc aaagcacatc aagttgagcg
     7681 tgtcatttgc agacttgaaa gcacaacgga cgatacagag ttagttgatt caggtattga
     7741 aattttcagt agctacttaa gtaataaaat cttattaaaa ggtttaattg aaacacctaa
     7801 catgttgaat ttattaagta atgttgaaac gtcactatat gaaattcaaa tgttaaatta
     7861 taaatatgaa aatattcaat tacgtaattt cccattcgga ggagacatca tcttcgtgcg
     7921 tattatccgt aataatgagt cgattgttcc gcatggagat acacaattgc gatatggaga
     7981 tcgcttaatt gttaccggtg ctaaagaata cgttgatgaa ttgaagcaag agttagaatt
     8041 ttatttttaa caataatgat attaaaatgc agacgtattt tagtacgacg taaaattaat
     8101 gattttaaaa tgctagtatg tatatgattt tgataaataa atgcttttta cgtaaatcaa
     8161 gtttgataca gaaaggacta aatcaaacat ttatcgttgt aatacgttta aataacttta
     8221 ttaaaaagtc ataatagtgt taaaatgtat tgacgaataa aaagttagtt aaaactggga
     8281 ttagatattc tatccgttaa attaattatt ataaggagtt atcttacatg ttaaatcttg
     8341 aaaacaaaac atatgtcatc atgggaatcg ctaataagcg tagtattgct tttggtgtcg
     8401 ctaaagtttt agatcaatta ggtgctaaat tagtatttac ttaccgtaaa gaacgtagcc
     8461 gtaaagagct tgaaaaatta ttagaacaat taaatcaacc agaagcgcac ttatatcaaa
     8521 ttgatgttca aagcgatgaa gaggttatta atggttttga gcaaattggt aaagatgttg
     8581 gcaatattga tggtgtatat cattcaatcg catttgctaa tatggaagac ttacgcggac
     8641 gcttttctga aacttcacgt gaaggcttct tgttagctca agacattagt tcttactcat
     8701 taacaattgt ggctcatgaa gctaaaaaat taatgccaga aggtggtagc attgttgcaa
     8761 caacatattt aggtggcgaa ttcgcagttc aaaactataa tgtgatgggt gttgctaaag
     8821 cgagcttaga agcaaatgtt aaatatttag cattagactt aggtccagat aatattcgcg
     8881 ttaatgcaat ttcagctggt ccaatccgta cattaagtgc aaaaggtgtg ggtggtttca
     8941 atacaattct taaagaaatc gaagagcgtg cacctttaaa acgtaatgtt gatcaagtag
     9001 aagtaggtaa aactgcggct tacttattaa gtgatttatc aagtggcgtt acaggtgaaa
     9061 atattcatgt agatagcgga ttccacgcaa ttaaataata tcattcaaca gctttgttca
     9121 cgttattata tatgtgagca aagctttttt gcttttataa taatcggact gatggaaaat
     9181 tatttgatat ttcatttgac tgatttttta tgcacaaaaa aatcgcatca aaagctgtag
     9241 tttcaacttt tgatgcgata gtatcttata ttcttaaaag acatccacag aattagtcct
     9301 taacattact gtttgcttta tcaataatgc gttggcggta tttgaaaata ttactaacaa
     9361 ccgttttaag tacagcatat aatggcactg caatcagaac taatgtaaag ccacctaaat
     9421 ctcctgctgc taaaataaca acgataattg ttaaaggatg gatacttaaa gatttaccca
     9481 ttacatttgg tgtaatgata ttaccttcaa gttgatgtgc aattaatgta atgatacaaa
     9541 cccatataaa tgtagtagga ctatctataa taccgagtat tgctgcaggt gcaaatgata
     9601 accatgaacc taagaaagga attaagtttg cgacaccagc aaatagtact aataaaggaa
     9661 tatatggtaa gtcaataatt gaataaccga tatataagaa tatacctaaa ataacactga
     9721 cagttacttg accttgaatg taagatttta atgtaaagtt taaatcagtt aataaatcta
     9781 cgaaaaatac tttacgttca cctttgaaaa atttagcaac agctgggata aatttttcat
     9841 ggtcttttaa catataaatt aagaagaatg gaaccataat caataagaag atggttgaaa
     9901 ttaatgatgt aatgtactgt aatgaattag ataaaatatt agtaacgcca tcacccattg
     9961 atttaaccat atttgtaatt ctacttgtta catcttcagg taatttatcc atttgagcta
    10021 gtgcgaattt aataatttgc tctgcttctt tttgtagggc aggtgtctga ctaattaaat
    10081 tgttgatatt tgaaatgatg attggtgcaa caaacgcaac aattaagccg ataatagcaa
    10141 acaaagctaa catgattgtt gttatactag cccatcttgg aaaaccgact ttttcaagta
    10201 agttttgaaa aggtagacag atataaaata aaaaaccact aattaaaaat ggaagaaata
    10261 cagaaccgat gattgtagct attggagtaa atacttcgtg cacttccata aatagtttga
    10321 tgagaatgaa cagcataatc agagcgatgc cagttcggaa ccaaaccttg tttaacataa
    10381 acgatgttcc tcctattttt ctaaacttag ttattattta gtatatccta aatagcagtg
    10441 cttaaaaagg ttaatatttt ttaaatatac ttaaattaac cattcacttt ctattttaag
    10501 atatttactt tcaatagttt gtgagacttt tcaatgactc gtaagtctgt aattggatca
//
LOCUS       DAXWFK010000091        10413 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.091, whole genome shotgun sequence.
ACCESSION   DAXWFK010000091 DAXWFK010000000
VERSION     DAXWFK010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10413
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.091"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            76..1494
                     /locus_tag="ACGEXS_001730"
     CDS             76..1494
                     /locus_tag="ACGEXS_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFF8012731.1"
                     /translation="MENTVKYRKFILPIVVGLLIWALTPFKPDAVDPTAWYMFAIFVA
                     TIIACITQPMPIGAVSIIGFTIMVLVGIVDMKTAVAGFGNNSIWLIAMAFFISRGFVK
                     TGLGRRIALHFVKLFGKKTLGLAYSIVGVDLILAPATPSNTARAGGIMFPIIKSLSES
                     FGSKPKDGSARKMGAFLVFTEFQGNLITAAMFLTAMAGNPLAQNLASSTSNVHITWMN
                     WFLAALVPGLVSLIVVPFIIYKIYPPTVKETPNAKSWAENELATMGKIALAEKFMIGI
                     FVVALTLWIVGSFIHIDATLTAFIALALLLLTGVLTWQDILNETGAWNTLVWFSVLVL
                     MADQLNKLGFIPWLSKSIATSLGGLSWPIVLVILILFYFYSHYLFASSTAHISAMYAA
                     LLGVAIAAGAPPLFSALMLGFFGNLLASTTHYSSGPAPILFSSGYVTQKRWWTMNLIL
                     GFVYFIIWIGLGSLWMKVIGIF"
     gene            complement(1907..2815)
                     /gene="rarD"
                     /locus_tag="ACGEXS_001731"
     CDS             complement(1907..2815)
                     /gene="rarD"
                     /locus_tag="ACGEXS_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFF8012732.1"
                     /translation="MNSEYKKGIFLALSAYILWGILPIYWQFVDAIGAFEILAFRIIF
                     SAIFMIFILAVGQKQRNAFQRDMNQLLGKPIQLLAIVVAGYVITLNWGTFIWAVTNGH
                     VLQTSLGYYINPLVSILLALIFLKERFNKFEWLAILFAFIGVLYMTLKIGEFPIVSII
                     LALSFGTYGLLKKVVHIDAISSITIECIVTAPAGLIYVIYLWQQHQMSFGLNMSSFWL
                     LFSGAITAIPLILFSAGAKRIPLSLIGFIQYVGPTIMFVLGIFVFKEPFSIDQLITFI
                     FIWTGIVLYSLSQYIKLKKHPVAKTL"
     gene            3040..3615
                     /locus_tag="ACGEXS_001732"
     CDS             3040..3615
                     /locus_tag="ACGEXS_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFF8012733.1"
                     /translation="MTLPKIGKPATRALNSQGIYTLEAVSQYTKSSLMEMHGVGPKAI
                     SILEQALFQHQLHFKTEVQSSLPFKLTGDVSCNHAPKRQQMIDFIVVTAALDIELLRS
                     LVTTEFIWSVPGRFDIYGPQILIQELSNHYNQVASLNIHSSITHGCLGSMHGIEILKT
                     GKEIHFAHFFEFENHKKDAKLSKVTSYIVVG"
     gene            3781..4797
                     /locus_tag="ACGEXS_001733"
     CDS             3781..4797
                     /locus_tag="ACGEXS_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HFF8012734.1"
                     /translation="MTVFKNERLSWLPYIAIVILLHVIGFSFLWIAGKDHHILFGMGI
                     LAYTLGLRHAFDADHIAAIDNTVRKLLQQRKDPSGVGFYFSIGHSSVVFLMAVFLGVS
                     VKWAKDELPHFQDIGGTIGTLVSGFFLVLIGVLNLIILISLINLFAKLRREHIEEAEV
                     DALLESRGLVSRFVGPYFKLITRSWHVLPLGFLFGLGFDTASEIALLALSSGASQQAI
                     SFIGILSLPILFASGMSLLDTLDGVVMKYAYNWAFFNPIRKIYYNITITAISVMAALV
                     IGMIELLQILADKLDLHGAFWAFIGSIEFDYLGYILVALFLITWLISSLIWKFGRIEH
                     KWSR"
     gene            5040..5834
                     /locus_tag="ACGEXS_001734"
     CDS             5040..5834
                     /locus_tag="ACGEXS_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HFF8012735.1"
                     /translation="MNIAKLENYLQIDSSRYNRPSIEALNYYATRFMLTVPFENIDVQ
                     NSKPISINIDALFNKIVHDKRGGFCYELNTFFKAYLQQKGFNPELMSATIHTPGGGRS
                     LNGSHASLVVSINDVFYVTDVGFGDLPLHAIPITSSEHTQPITDISGTFRAIFNNEDK
                     DIFYVQKFENDHWHTKYEAEFKPKQIEDFNSNIEYNQTHPDSIFVQHLLITMPQSFGR
                     ATMSENHLTLTRNGSSEKFDVTKDNYKHFLEKYFGLNVTINRIEKQ"
     gene            complement(5968..6480)
                     /locus_tag="ACGEXS_001735"
     CDS             complement(5968..6480)
                     /locus_tag="ACGEXS_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFF8012736.1"
                     /translation="MEKGNQGIKWSSLIMGVLLLMLAVVIFTFPIENFYAITWLIGLF
                     VLINGVIQIVYRRKAKALVGGNQNWILFMGIVDILFGLLVIFNVGASSAFFIYMFAFW
                     FIFSSISGLFTFSGSGSLKLISVIFNLLGIVFGVILLFNPLMGIVFISTMIAIAFVFV
                     GVIYVVDALA"
     gene            6763..7521
                     /gene="vraD"
                     /locus_tag="ACGEXS_001736"
     CDS             6763..7521
                     /gene="vraD"
                     /locus_tag="ACGEXS_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter ATP-binding
                     subunit VraD"
                     /protein_id="HFF8012737.1"
                     /translation="MTILSVQHVSKTYGKKHTFQALKDINFDIQKGEFVAIMGPSGSG
                     KTTLLNVLSSIDQISSGSVIANGQELNKLNQKALAKFRKESLGFIFQDYSILPTLTVK
                     ENIMLPLSVQKMSKATMEENYKAITTALGIYDLGNKYPSELSGGQQQRTAAARAFVHK
                     PQIIFADEPTGALDSKSANDLLQRLEEMNKSFDTTIVMVTHDPVAASFAERVIMLKDG
                     QIHTQLYQEGRSKQAFYEDIVHLQSVLGGVSNDI"
     gene            7511..9391
                     /gene="vraE"
                     /locus_tag="ACGEXS_001737"
     CDS             7511..9391
                     /gene="vraE"
                     /locus_tag="ACGEXS_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter permease
                     subunit VraE"
                     /protein_id="HFF8012738.1"
                     /translation="MTFNHIVFKNLRQNLKHYAMYLFSLFFSIVLYFSFTTLQFTKGV
                     NNDDSMAIIKKGALVGSIFLFIIIVIFLMYANHLFVKRRTREFALFQLIGLTRQNILK
                     MLALEQMIVFLITGVVGVLCGIAGAQLLLSIVSKLMSLSINLSIHFEPMALVLTIFML
                     IIAYVLILFQSALFLKRRSILSMMKDSIKTDATTAKVTTAEVISGVLGIAMIALGYYM
                     ATEMFGTFKALTMAMTSPFIILFLTVVGAYLFFRSSVSLIFKTLKKSKNGRVSITDVV
                     FTSSIMYRMKKNAMSLTIIAIISAVTVTVLCFAALSKSNTDQTLTSMAPNEFNVVATQ
                     DAKQFETKLSQQQITFSKNAYETITVDNVNDQVITLENGSDSGRTNSILSANNKLTGN
                     NAIITNTKSLPNIINIHLNKDLVVKGTKNETFRVTQEDKGKVYPLNLSFNSPVIEVSP
                     EKYQQLKTQNNVHTFYGYDIKQTSQKEKAQAIAKQFGDKVITYDEMKKEVDATNGILI
                     FVTSFLGLAFLVAAGCIIYIKQMDETEDELSNFRILKRIGFTHTDMLKGLLLKITFNF
                     GLPLLIAILHAVFAAIAFMKLMGNISFMPVIVVIVVYTLIYITFALIAFVHSNKLIKK
                     TI"
     gene            9484..9675
                     /gene="vraH"
                     /locus_tag="ACGEXS_001738"
     CDS             9484..9675
                     /gene="vraH"
                     /locus_tag="ACGEXS_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter activity
                     modulator VraH"
                     /protein_id="HFF8012739.1"
                     /translation="MKFRELVKQSYEDLKNLTVTWYNVIALTILVIVLSSFTTPMIGI
                     PAGLLGGAYYLKRREEKGK"
     gene            9726..10223
                     /locus_tag="ACGEXS_001739"
                     /pseudo
     CDS             9726..10223
                     /locus_tag="ACGEXS_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(10283..>10413)
                     /locus_tag="ACGEXS_001740"
     CDS             complement(10283..>10413)
                     /locus_tag="ACGEXS_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFF8012740.1"
                     /translation="IVFYNQENIFYISNAVIDIISVSKDTLDYIKSEFNDHCASPY"
BASE COUNT         3475 a         1774 c         1673 g         3491 t
ORIGIN      
        1 actgtacatc ttttgtagtt agctttattc ttaaattgaa atatgtataa aacatagaaa
       61 gaaggacgtt ttaacatgga aaacacggtt aaatatcgta agtttatact ccctatcgtt
      121 gtaggtctcc ttatttgggc acttacacct tttaaaccgg atgctgtgga tccaacagca
      181 tggtatatgt tcgcaatatt cgtcgcgaca atcattgctt gtattacaca accgatgcca
      241 attggggccg tctctataat tggatttaca atcatggtac tcgttggcat tgttgacatg
      301 aaaacggctg tcgctggttt tggtaataat agcatttggt taattgctat ggcatttttc
      361 atttcgagag gatttgtgaa aacaggtctt ggtagacgta tcgcacttca tttcgtcaaa
      421 ttatttggta aaaaaacatt aggattagca tattctatcg tcggtgtaga tttaattcta
      481 gcgcctgcta caccaagtaa taccgcgcgt gctggtggaa tcatgttccc aattatcaaa
      541 tcactttctg aatcatttgg ttcgaaaccg aaagacggat cagcacgcaa aatgggtgca
      601 tttcttgttt tcacagaatt ccaaggtaat ttaattactg cggctatgtt tttaactgca
      661 atggccggta acccccttgc acaaaattta gcatctagca catctaatgt tcacattaca
      721 tggatgaatt ggtttctagc tgctttagtt cctggacttg tttccttaat tgttgtacct
      781 tttattattt ataaaattta tccaccaact gttaaagaaa caccaaatgc taagagttgg
      841 gctgaaaatg aattagcgac tatgggtaaa atcgctttag ctgaaaaatt tatgattggt
      901 atttttgtcg ttgcgttaac actatggatt gtcggaagtt tcattcatat tgatgcaact
      961 ttaacggcct ttattgcgct agcattgtta ttattgacag gcgtcttaac atggcaagac
     1021 attttaaacg aaacaggtgc ttggaacaca ttagtatggt tctcagtatt agtgttaatg
     1081 gccgaccaat taaacaagct tggatttatt ccttggttaa gtaaatccat tgctacaagt
     1141 cttggtggct taagctggcc tatagtcctg gtcattttaa tattgttcta cttctattca
     1201 cattacttat ttgcaagttc tacagcacat atcagtgcga tgtatgcagc attactaggc
     1261 gttgccatcg cagccggtgc accaccatta ttcagtgcat taatgttagg tttcttcggt
     1321 aacctattag cttcaacaac acactatagt agtggtccag cgccgattct attctcttca
     1381 ggttacgtga ctcaaaaacg ttggtggaca atgaacttaa tattaggttt cgtctacttt
     1441 attatctgga ttggtttagg atcactttgg atgaaagtaa ttggtatatt ttaaaatatt
     1501 taaattagcg ctcgaatctc attgatttgg gcgcttttta atttgtattt aaaatcaacc
     1561 tttgctaaat caagactccc tttttaaaat acgtttatcc tttaaatcat tgcgtgcttc
     1621 actgaaaatt tgtataaaga tttaagtcat tacgtaacat cacataaaat acatttctat
     1681 actattccgc ttcattgatt aacattacgt atgccctcat aaatcatcat acaaaaaaca
     1741 ccttcgttta aattcatttt aattgcgaat tcaacgaaag tgccttattt catatttaat
     1801 gtttcaaatt tatacgtctg tcactgttac tgcacacata cctcagtctc acctcatcat
     1861 gacacaaagg atattgaaaa cacgtataat tatctttcct cgtattttat agggtttttg
     1921 cgaccggatg tttcttcaat ttaatgtatt gagaaagact atataacaca atacctgtcc
     1981 aaataaatat aaacgtaatt aattgatcta tactaaaagg ctctttgaaa acaaatatgc
     2041 cgagtacaaa cattattgtt ggtccaacgt attgaataaa tcctattagc gaaagtggaa
     2101 tacgttttgc cccggctgag aataggatta gtggtattgc cgtaatagca ccagaaaata
     2161 acaaccaaaa tgatgacatg ttcaatccaa atgacatctg atgttgctgc cataaataaa
     2221 taacgtatat tagtccagca ggtgcggtaa caatacattc aatcgtaata ctgctgatgg
     2281 catcaatatg tactactttt ttcaataatc cgtatgtacc aaaggataac gctaatataa
     2341 tagagacgat tgggaattct ccaatcttga gcgtcatata taatacaccg atgaatgcga
     2401 ataaaatggc tagccattca aatttattga atctttcttt taaaaagata agtgcgagca
     2461 aaatgctaac aagtggattt atataataac ctaaacttgt ttgtaggacg tgaccgttcg
     2521 ttacagccca aataaatgta ccccaattta atgtaatgac atagcctgct acgacaatcg
     2581 ctaatagctg aatgggcttg cctaacaatt gattcatatc tcgttgaaat gcattgcgtt
     2641 gtttttgtcc aaccgcgagt atgaaaatca tgaatattgc tgaaaatata atacgaaagg
     2701 ctaaaatttc aaatgcgcct attgcatcaa cgaactgcca atatataggt agtattcccc
     2761 acagaatgta tgcactgagt gctaaaaata tgcctttttt atactctgaa ttcaccttca
     2821 aacctcctta ctttcctaat ttttaattta ctgcatacgc tcacttggtt atgctaatat
     2881 aacgatttta ctaataatat ttcgataaag atatcatttt gtttatattt cccacattta
     2941 ttcaccaacc actaaacaat attaatttta taaataattc tgtacaaatc agggtatatt
     3001 gccagaaaga ctaccataca acataaagga tggatacaaa tgactttacc taaaattgga
     3061 aagcctgcaa cacgcgcgct aaattcacaa ggtatataca cattagaagc agtatcacaa
     3121 tatacgaagt catctctaat ggagatgcat ggcgttggtc ctaaagctat atcaatattg
     3181 gaacaagctt tatttcagca ccaactacat tttaaaacgg aagttcagtc atcattacct
     3241 tttaagttaa caggagatgt gtcatgcaat catgcgccta aacgtcaaca aatgatagat
     3301 tttatagttg ttacagcagc attagatatt gaattattac gttcactggt tacaacagaa
     3361 tttatttggt ctgtccctgg ccgctttgac atatacggac ctcaaatatt aattcaagaa
     3421 ctatctaatc attacaatca ggttgctagt ctcaatatcc attccagcat cacacatggg
     3481 tgcttaggtt ccatgcatgg tattgaaata ttaaaaactg gtaaagagat tcattttgct
     3541 catttttttg aatttgaaaa tcataaaaag gatgccaagt taagcaaagt gacatcttac
     3601 attgttgttg gttaatttat caaattatat atatcgcaaa aaatcttttg tggttacatg
     3661 tatatttttt atataacaaa taatttggca taacattttt gttatatgaa cacttattac
     3721 tagagtgccc attgaacata cagtatgatt aaatatcatg ttatgaaaga aggatatatt
     3781 ttgactgttt ttaaaaatga gcgcttaagc tggttaccat acatagctat tgtcattttg
     3841 ttacacgtta ttgggtttag ttttttatgg attgctggaa aagaccatca tatcttattt
     3901 ggtatgggga ttcttgcata tacattaggt ttgcgtcatg catttgatgc agatcacatt
     3961 gctgcaatag ataatacggt tcgcaaatta ttacagcaac gcaaagatcc atctggtgtg
     4021 ggtttctatt tttcaattgg acattcatct gtcgtatttt taatggctgt ttttttaggg
     4081 gtatctgtaa aatgggctaa agatgaatta ccgcatttcc aagatattgg tggaacgatt
     4141 ggtacactag tttcaggttt ctttttagtg cttatcggtg tgttgaatct aattatttta
     4201 atctctttaa ttaacttgtt cgctaaatta cgtcgtgaac acattgaaga agctgaagtc
     4261 gatgcattac ttgaatctag aggattggtt tctcgatttg taggacctta tttcaaatta
     4321 atcacgcgta gttggcacgt attgccactt ggctttttat ttggacttgg ttttgataca
     4381 gctagtgaaa ttgcgttact cgctctttct tcaggcgcat cacaacaagc catttcattt
     4441 atcggaatct tatctttacc aattttattc gcatcaggta tgagtttatt ggatacatta
     4501 gatggtgttg taatgaagta tgcctataat tgggcattct tcaatcctat tcgcaaaatc
     4561 tattacaata taacgattac tgcgatatca gtcatggcag cattagtgat tgggatgatt
     4621 gaattgctac aaattcttgc tgataagtta gatttacatg gtgcgttttg ggcattcatt
     4681 ggttcgatcg aatttgatta tttaggctat attttagttg cattattttt aattacttgg
     4741 cttatttcaa gtttaatttg gaagtttggt cgcatagaac acaaatggtc tagataaggc
     4801 aacttgtctt gcaactacct ttgtattgtt gcatttttag acaccctgca cacttcttcc
     4861 attatgctac gctctcagta gtcatcttta aatcgtttct tttagagatg acttcatttt
     4921 gagagcgtat ttttctattt aaaatgtatt tatccaaaat tcaatgcttt cgaatttaaa
     4981 atttcagtat ttctgtttaa aatttaatta taatctttaa tacaggaggc gctatgctta
     5041 tgaatattgc gaagttagag aattatttac aaattgattc atctcgttat aatcgaccga
     5101 gtattgaagc attaaattat tatgcaacac gttttatgtt aactgtacct tttgaaaata
     5161 ttgatgttca aaatagtaag ccgatatcta taaatatcga tgcacttttt aataaaattg
     5221 ttcatgataa acgtggtggt ttttgttatg aattaaatac atttttcaaa gcctatttac
     5281 aacaaaaagg atttaatcct gaattaatgt cagctactat tcacacaccc ggtggcggtc
     5341 gtagtctgaa cggttcacat gcttcacttg ttgtttctat aaatgatgta ttctatgtaa
     5401 ctgatgttgg ctttggagac ttacctctac atgccattcc tattacttca tctgagcata
     5461 cacaaccaat tactgatatc agtggtacat tccgcgctat ttttaacaac gaagacaagg
     5521 atattttcta tgttcaaaaa ttcgaaaatg atcattggca tacaaaatat gaagctgaat
     5581 tcaaacctaa acaaatagaa gattttaata gcaacatcga atataatcaa acgcatccag
     5641 attcaatatt tgtacagcat ttattaatta caatgccaca atcatttggc cgtgctacaa
     5701 tgtctgaaaa tcatttaact ttaacaagga atggtagctc agaaaaattt gatgtcacta
     5761 aagataacta taaacatttt ttagaaaaat attttggact aaatgtaact attaatcgta
     5821 ttgaaaaaca ataatagtaa aatataaaaa tatgggcgta tcttacttat tgtaggtagc
     5881 gccccttttt tatgcttcat aaacacaaca aaaaggatat gacacaaact tcatgtcaca
     5941 cccttctttt gaaccgcttc attttactta agcaagtgca tctacaacat aaatgacacc
     6001 tacgaataca aatgcaatag caatcatcgt cgaaataaag acgataccca ttaatggatt
     6061 aaataataaa atgacaccga aaacaatacc taataaatta aaaatcactg aaattagttt
     6121 taagctacca ctacccgaaa acgtaaataa tccagagata gaactaaaaa taaaccaaaa
     6181 agcaaacata taaataaaga atgctgaact tgcgccaaca ttaaaaataa ctaatagacc
     6241 aaatagaata tctacaatcc ccataaacag aatccaattt tggttaccac ctactaaagc
     6301 ttttgctttt ctacggtaaa cgatttgaat cacaccgtta attaatacaa acagtccaat
     6361 caaccaggta atagcataaa aattttcaat tggaaatgta aaaataacga ctgccaacat
     6421 taataataat acacccatta ttaaactaga ccatttaata ccttgatttc ctttttccat
     6481 aacaatcaat tcctttcaaa taaaaccctc ttttaatttt tacaaaaaat ttattataca
     6541 ataattaaat tcccttttct caatttacac aaacatcatc acttagaaag caccattgct
     6601 tttgtctaat atgtacgatg aatctgtatc ctcactcaaa aatctactaa gtagcgatag
     6661 ctttcaactc tgtaaggttc attcattgaa ttgtaagttt agataaaggc tactactcca
     6721 aacattattt aaaatgaaat taaatacaaa aggagtgaga ctatgacgat attatcagtg
     6781 caacatgttt caaaaacata cggtaaaaag cacacatttc aagcacttaa agatattaac
     6841 tttgacatac aaaaaggcga attcgttgcg attatggggc cttctggatc aggtaagaca
     6901 accttattaa atgtactaag ttcaattgac caaatttcta gcggtagcgt gattgctaac
     6961 ggacaagagc ttaataaact taatcaaaaa gcacttgcca aattccgcaa agaatcatta
     7021 ggtttcatct tccaagatta cagtattctg ccgacattaa ccgttaaaga aaacattatg
     7081 ttacctttat ctgttcaaaa aatgtcgaag gcaacaatgg aagaaaatta taaagcgatc
     7141 acgacagcat taggtattta tgacctagga aataaatacc ctagcgaatt atctggtggt
     7201 caacaacaaa gaactgcagc agcgagagca tttgttcaca aaccacaaat catatttgca
     7261 gatgagccaa caggcgcact cgactcgaaa agtgcaaatg acctattaca acgtttggaa
     7321 gaaatgaata aatcgtttga tacaactatt gtcatggtta cacatgatcc ggttgcagca
     7381 agttttgcag aacgtgtcat catgctaaaa gatggccaaa ttcatacaca actttatcag
     7441 gaaggacgtt ctaaacaggc cttttatgaa gacattgtac atcttcaatc agtattaggt
     7501 ggtgtctcaa atgacattta accatatcgt tttcaaaaac ttacgacaaa acttaaaaca
     7561 ttatgcaatg tatttatttt cattattttt tagcatcgtc ttatatttca gttttacaac
     7621 cttacagttt actaaaggtg taaataatga cgactcgatg gccatcatta agaaaggtgc
     7681 tttagtcgga tcaatatttt tatttatcat tattgtcatc tttttaatgt atgccaatca
     7741 tttattcgta aaacgccgta cacgtgaatt tgcgctattt cagttgattg gtttaacacg
     7801 acaaaacatt ttgaaaatgt tagcacttga acaaatgatc gtatttttaa tcacaggtgt
     7861 tgtcggtgta ttatgtggca ttgcaggtgc acaattattg ctttccatag tatctaaatt
     7921 gatgtcgtta tcgattaact tatcgataca tttcgaacct atggcccttg ttttaactat
     7981 tttcatgcta attattgcgt atgtactgat tttatttcaa agtgctttat ttctaaaaag
     8041 acgtagtatc ttatcaatga tgaaagattc aattaaaact gatgccacaa ctgctaaagt
     8101 aacgactgca gaggttattt caggcgtatt aggtattgct atgattgcac taggctatta
     8161 tatggcgaca gaaatgtttg gtacgttcaa agcactaact atggctatga catcaccgtt
     8221 tatcatttta tttttaacgg ttgtaggcgc ctatttattc ttcagaagtt ccgtgtcact
     8281 tatttttaaa acattgaaaa aatcaaaaaa tggacgcgta tctattaccg atgttgtatt
     8341 cacatcgtct attatgtaca gaatgaagaa aaatgccatg tctttaacta tcattgcaat
     8401 tatttctgca gttacagtaa ctgtattgtg ttttgcggca ttatctaaat caaatacaga
     8461 tcaaaccctt acatctatgg caccaaatga atttaacgtg gtcgctactc aagatgctaa
     8521 acaatttgaa actaaactaa gccaacaaca aattacattt tctaaaaatg cttacgaaac
     8581 tattactgta gataatgtta atgatcaggt tattactttg gaaaatggta gtgattcagg
     8641 tcgcacgaat tctattttaa gtgcaaataa caagttaaca ggcaacaatg ccatcattac
     8701 aaatacaaaa tcgctgccta acattattaa tatccattta aacaaagatt tagtagtaaa
     8761 aggtactaag aatgaaactt tccgtgttac acaagaagac aaaggtaagg tttatccttt
     8821 aaatctaagc ttcaactcac ctgtcatcga agtaagtcct gaaaagtatc agcagttgaa
     8881 aacacaaaat aacgttcata ctttttatgg atatgatatt aaacaaacat cacaaaagga
     8941 aaaagctcaa gcaattgcaa aacagtttgg agacaaagtc atcacgtatg atgaaatgaa
     9001 aaaagaggtc gatgctacta acggtattct aatatttgtt acatcatttt taggattagc
     9061 atttttagta gcagctggat gtattattta tattaaacaa atggatgaaa ctgaggatga
     9121 actaagtaac ttcagaatat taaaacgcat aggctttacg catacagata tgcttaaggg
     9181 attattatta aaaattactt tcaacttcgg tttaccatta ttgattgcta tattacatgc
     9241 agtattcgcc gcaatagcat ttatgaaatt gatgggtaat atttccttca tgccggttat
     9301 tgtagtaatt gttgtataca ccctcattta cattactttt gctttaattg cttttgtaca
     9361 ttcaaacaaa ttgattaaga aaaccattta acacttacga ttaaaagatg cttcattaga
     9421 tattttgatt ttaagcaatg cagtataact attaattgat ttaagaaaag gagagattga
     9481 atcatgaaat ttagagaatt agtaaaacaa tcatatgagg atttgaaaaa tttgactgta
     9541 acttggtata acgtcattgc tttaacaata cttgttattg tattaagctc attcacaaca
     9601 ccaatgatag gcattccagc tggtttatta ggtggcgcct attatttaaa acgtcgtgaa
     9661 gaaaaaggta aataaataag ccttatgtgc taaactcaat atgtatttcc taccaaagaa
     9721 aacataagga gtttggacat cttgaaggag atactattgt tggtcgtcgt catggtagtg
     9781 caatcattac tttggtagaa agagtatcca agatgattat caccttacga cccttaggtc
     9841 gtaaggcaac tgatatagaa aacgctctac actcaatgtt ttcatcttta ccttctcata
     9901 tatttaaatc aattactttt gattgtggta aggagttctc aaactggaaa acattatgta
     9961 actgtcacga tatatctata ttttttgctg atccaggcac gccatcacag cgaggtttaa
    10021 atgaacattc taacggaata cttagacgca gtggattaga taaagaacta gatttcaatt
    10081 tagtaacaga cgatcatatc atcagtgttg ctcaaaaaat caatcatcgt cctagaaaat
    10141 ctttaggtta tagaacacca ttggaagtat ttatgagttt catcgaagat gataaacttg
    10201 tccagcttaa tttgacaatc taaagattta taaaaaagcc gttggtctct tcgaaactac
    10261 ggcttttctc tgtgctataa aatcagtatg gcgaagcaca gtgatcattg aattctgact
    10321 ttatataatc aagagtatct ttggatacac tgatgatatc tataacagca ttgctaatgt
    10381 agaatatatt ttcttgatta tagaatacaa taa
//
LOCUS       DAXWFK010000092        10310 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.092, whole genome shotgun sequence.
ACCESSION   DAXWFK010000092 DAXWFK010000000
VERSION     DAXWFK010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10310
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.092"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(58..150)
                     /locus_tag="ACGEXS_001741"
     CDS             complement(58..150)
                     /locus_tag="ACGEXS_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012741.1"
                     /translation="MTKNSKYTNEVSKEQFQEIENVNNKVKEFY"
     gene            114..248
                     /locus_tag="ACGEXS_001742"
     CDS             114..248
                     /locus_tag="ACGEXS_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012742.1"
                     /translation="MKLHWYIYYFLSISVILFMIIYHYLATSMTVDYEITVSIAKLLL
                     "
     gene            complement(453..932)
                     /gene="rlmH"
                     /locus_tag="ACGEXS_001743"
     CDS             complement(453..932)
                     /gene="rlmH"
                     /locus_tag="ACGEXS_001743"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020363758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFF8012743.1"
                     /translation="MKITILAVGKLKEKYWKQAIAEYEKRLGPYTKIDIIEVPDEKAP
                     ENMSDKEIEQVKEKEGQRILAKIKPQSTVITLEIQGKMLSSEGLAQELNQRMTQGQSD
                     FVFVIGGSNGLHKDVLQRSNYALSFSKMTFPHQMMRVVLIEQVYRAFKIMRGEAYHK"
     gene            complement(1300..3618)
                     /gene="adsA"
                     /locus_tag="ACGEXS_001744"
     CDS             complement(1300..3618)
                     /gene="adsA"
                     /locus_tag="ACGEXS_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored adenosine synthase AdsA"
                     /protein_id="HFF8012744.1"
                     /translation="MKALLLKTSVWLVLLFSVMGLWQVSNAAEQHTPMKAHAVTTIDK
                     ATTDKQQVPPTKEAAHHSGKEAATNVSASAQGTADDTNSKVTSNAPSNKPSTVVSTKV
                     NETRDVDTQQASTQKPTHTATFKLSNAKTASLSPRMFAANAPQTTTHKILHTNDIHGR
                     LAEEKGRVIGMAKLKTVKEQEKPDLMLDAGDAFQGLPLSNQSKGEEMAKAMNAVGYDA
                     MAVGNHEFDFGYDQLKKLEGMLDFPMLSTNVYKDGKRAFKPSTIVTKNGIRYGIIGVT
                     TPETKTKTRPEGIKGVEFRDPLQSVTAEMMRIYKDVDTFVVISHLGIDPSTQETWRGD
                     YLVKQLSQNPQLKKRITVIDGHSHTVLQNGQIYNNDALAQTGTALANIGKITFNYRNG
                     EVSNIKPSLINVKDVENVTPNKALAEQINQADQTFRAQTAEVIIPNNTIDFKGERDDV
                     RTRETNLGNAIADAMEAYGVKNFSKKTDFAVTNGGGIRASIAKGKVTRYDLISVLPFG
                     NTIAQIDVKGSDVWTAFEHSLGAPTTQKDGKTVLTANGGLLHISDSIRVYYDINKPSG
                     KRINAIQILNKETGKFENIDLKRVYHVTMNDFTASGGDGYSMFGGPREEGISLDQVLA
                     SYLKTANLAKYDTTEPQRMLLGKPAVSEQPAKGQQGSKGSKSGKDTQPIGDDKVMDPA
                     KKPAPGKVVLLLAHRGTVSSGTEGSGRTIEGATVSSKSGKQLARMSVPKGSAHEKQLP
                     KTGTNQSSSPEAMFVLLAGIGLIATVRRRKAS"
     gene            complement(3846..4646)
                     /locus_tag="ACGEXS_001745"
     CDS             complement(3846..4646)
                     /locus_tag="ACGEXS_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFF8012745.1"
                     /translation="MSRLIRMSVLASGSTGNATFVENEKGSLLVDVGLTGKKMEELFS
                     QIDRNIQDLNGILVTHEHIDHIKGLGVLARKYQLPIYANEKTWQAIEKKDSRIPMDQK
                     FIFNPYETKSIAGFDVESFNVSHDAIDPQFYIFHNNYKKFTILTDTGYVSDRMKGMIR
                     GSDAFIFESNHDVDMLRMCRYPWKTKQRILGDMGHVSNEDAAHAMTDVITGNTKRIYL
                     SHLSQDNNMKDLARMSVGQVLNEHDIDTEKEVLLCDTDKAIPTPIYTI"
     gene            complement(5036..5824)
                     /locus_tag="ACGEXS_001746"
     CDS             complement(5036..5824)
                     /locus_tag="ACGEXS_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFF8012746.1"
                     /translation="MNWKLTKTLFIFVFILVNIVLVSIYVNKVNRSHINEVESNNEVN
                     FQQEEIKVPTSILNKSVKGIKLEQITGRSKDFSSKAKGDSDLTTSDGGKLLNANISQS
                     VKVSDNNLKDLKDYVNKRVFKGAEYQLSEISSDSVKYEQTYDDFPILNNSKAMLNFNI
                     EDNKATSYKQSMMDDIKPTDGADKKHQVIGVRKAIEALYYNRYLKKGDEVINARLGYY
                     SVVNETNVQLLQPNWEIKVKHDGKDKTNTYYVEATNNNPKIINH"
     gene            complement(5825..7159)
                     /gene="yycH"
                     /locus_tag="ACGEXS_001747"
     CDS             complement(5825..7159)
                     /gene="yycH"
                     /locus_tag="ACGEXS_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFF8012747.1"
                     /translation="MNNKEHIKSVILALLVLMSVVLTYMVWNFSPDIANVDNTDSKKS
                     ETKPLTTTMTAKMDTTITPFQIIHSKNDHPEGTIATVSNVNKLTKPLKNKEVKSVEHV
                     RRDHNLMIPDLNSDFILFDFTYDLPLSTYLGQVLNMNAKVPNHFNFNRLVIDHDADDN
                     IVLYAISKDRHDYVKLTTTTKNDHFLDALAAVKKDMQPYTDIITNKDTIDRTTHVFAP
                     SKPEKLKTYRMVFNTISVEKMNAILFDDSTIVRSSKSGVTTYNNNTGVANYNDKNEKY
                     HYKNLSEDEASSSKMEETIPGTFDFINGHGGFLNEDFRLFSTNNQSGELTYQRFLNGY
                     PTFNKEGSNQIQVTWGEKGVFDYRRSLLRTDVVLNSEDNKSLPKLESVRSSLANNSDI
                     NFEKVTNIAIGYEMQDNSDHNHIEVQINSELVPRWYVEYDGEWYVYNDGRLE"
     gene            complement(7152..8978)
                     /gene="walK"
                     /locus_tag="ACGEXS_001748"
     CDS             complement(7152..8978)
                     /gene="walK"
                     /locus_tag="ACGEXS_001748"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFF8012748.1"
                     /translation="MKWLKQLQSLHTKLVIVYVLLIIIGMQIIGLYFTNNLEKELLDN
                     FKKNITQYAKQLEISIEKVYDEKGSVNAQKDIQNLLSEYANRQEIGEIRFIDKDQIII
                     ATTKQSNRSLINQKANDSSVQKALSLGQSNDHLILKDYGGGKDRVWVYNIPVKVDKKV
                     IGNIYIESKINDVYNQLNNINQIFIVGTAISLLITVILGFFIARTITKPITDMRNQTV
                     EMSRGNYTQRVKIYGNDEIGELALAFNNLSKRVQEAQANTESEKRRLDSVITHMSDGI
                     IATDRRGRIRIVNDMALKMLGMAKEDIIGYYMLSVLSLEDEFKLEEIQENNDSFLLDL
                     NEEEGLIARVNFSTIVQETGFVTGYIAVLHDVTEQQQVERERREFVANVSHELRTPLT
                     SMNSYIEALEEGAWKDEELAPQFLSVTREETERMIRLVNDLLQLSKMDNESDQINKEI
                     IDFNMFINKIINRHEMSAKDTTFIRDIPKKTIFTEFDPDKMTQVFDNVITNAMKYSRG
                     DKRVEFHVKQNPLYNRMTIRIKDNGIGIPINKVDKIFDRFYRVDKARTRKMGGTGLGL
                     AISKEIVEAHNGRIWANSVEGQGTSIFITLPCEVIEDGDWDE"
     gene            complement(8991..9692)
                     /gene="yycF"
                     /locus_tag="ACGEXS_001749"
     CDS             complement(8991..9692)
                     /gene="yycF"
                     /locus_tag="ACGEXS_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFF8012749.1"
                     /translation="MARKVVVVDDEKPIADILEFNLKKEGYDVYCAYDGNDAVDLIYE
                     EEPDIVLLDIMLPGRDGMEVCREVRKKYEMPIIMLTAKDSEIDKVLGLELGADDYVTK
                     PFSTRELIARVKANLRRHYSQPAQDTGNVTNEITIKDIVIYPDAYSIKKRGEDIELTH
                     REFELFHYLSKHMGQVMTREHLLQTVWGYDYFGDVRTVDVTIRRLREKIEDDPSHPEY
                     IVTRRGVGYFLQQHE"
BASE COUNT         2897 a         2025 c         1560 g         3828 t
ORIGIN      
        1 ttttccaaat agtttattaa aaaactacat ctttcttgat tgatactctt tcaaatctta
       61 ataaaattct ttgaccttat tatttacatt ctcaatttct tggaattgtt cttttgaaac
      121 ttcattggta tatttactat tttttgtcaa tatctgtaat tttatttatg attatttatc
      181 attacttagc tacgtcaatg actgttgatt atgaaataac tgtttctatt gcaaagttac
      241 ttttataatt taataaggac aaaaagaagc attctatatt aatcatttta gatataaacc
      301 aatttgatag ggcctaattt caactgttag ctactactta agttatatgc gcaattatcg
      361 tgatatatct tatatattga atgaacgtgg atttaatgtc caccatttaa caccctccaa
      421 attattatct cctcatacag aattttttag ttttacttat gatacgcctc tcctcgcata
      481 atcttaaatg ctctgtacac ttgttcaatt aacacaaccc gcatcatttg atgtgggaat
      541 gtcattttgc tgaatgatag tgcgtagtta ctgcgttgta agacgtcctt gtgcaggccg
      601 tttgatccgc caatgacgaa aacaaagtcg ctttgccctt gggtcatgcg ttggttcaat
      661 tcttgggcca atccttcgga agatagcatc tttccttgta tttctaatgt aatgactgtg
      721 gattgtggtt tgattttggc tagtattcgt tggccttctt tttcttttac ttgctcaatt
      781 tctttgtcac tcatattttc tggtgctttt tcgtctggaa cttctatgat gtctatcttg
      841 gtgtatgggc ctaaacgttt ttcatattct gctatggctt gcttccaata tttctctttt
      901 agtttcccta cagctaaaat ggtgattttc atgtcgtttg gtcctccaaa ttgttatcaa
      961 ctttccagtt atccacaagt tattaacttg ttcacactgt tccctcttat tataccaata
     1021 ttttttgcag tttttgatat tttcctgaca tttatgcccg atttttagtg tataacctgt
     1081 gtatttgtgg ataactctgt gggcattgtg ggtatttctc tttaaattgt acacaattct
     1141 ggataactat ccccatttct gtggataaac accttgatgt cttattactt atcctcgttt
     1201 ttatatccca tatttatcaa cggttatcgc tttttttcat aaaaaaacac taccgtacct
     1261 cttatttaag aaatacagta gtattatttt caatatattt tagctagctt ttctacgtcg
     1321 tacagtcgcg attaaaccta tacctgctaa taatacaaac atcgcttctg ggcttgaact
     1381 ttgattagtt ccagtttttg gtaactgttt ctcatgcgcg ctacctttag gcactgacat
     1441 tctagccaat tgtttcccac tcttgcttga tacagtagct ccttctattg tgcgaccaga
     1501 accttctgta ccgctactaa cagttcctct atgcgctagc aacaatacaa ctttacctgg
     1561 agctggtttt ttcgctggat ccatcacttt gtcgtcacca attggttgtg tatctttacc
     1621 agacttacta cctttgctac cttgttgtcc tttagctggt tgttcactta ctgctggttt
     1681 acctaataac atacgttgtg gttctgtcgt atcatactta gctaagttag ctgtttttaa
     1741 ataacttgct agtacttgat ctaatgaaat accttcttct ctaggaccac cgaacatact
     1801 atatccgtcg ccacctgatg ctgtgaagtc attcatcgtt acgtgatata cacgttttaa
     1861 atcaatattt tcaaacttac ctgtctcttt atttaaaatt tgaatagcat taattcgttt
     1921 gccagacggt ttatttatat catagtaaac acggattgaa tcagagatat gtagtaaacc
     1981 gccattcgct gttaacactg tcttaccgtc cttttgtgtt gttggtgcgc ctaaactatg
     2041 ttcgaaagcc gtccagacgt ctgaaccttt tacatcaatt tgcgcaatcg tatttccaaa
     2101 tggtaatact gagattaaat catagcgtgt caccttacct tttgcgatag aggcacgaat
     2161 acctccacca tttgtcacgg caaagtcagt ctttttagag aaattcttaa cgccatacgc
     2221 ttccatagca tctgcaatcg cgtttcctaa atttgtttca cgcgttctaa cgtcatctct
     2281 ttctcctttg aaatcaatgg tattgtttgg aataattacc tctgcagttt gtgctctaaa
     2341 tgtttgatca gcttgattaa tttgttcagc taatgctttg ttcggtgtta cattttcaac
     2401 gtctttaaca ttaatcaatg acggtttaat attcgatacc tctccattgc gataattaaa
     2461 tgtaatctta ccgatattcg caagtgctgt acctgtttgt gccaatgcat cattgttata
     2521 aatttgacca ttttgaagta ctgtatgtga atgaccatca ataactgtaa tacgtttctt
     2581 caattgtgga ttttgactta attgtttcac taagtaatca ccacgccatg tttcttgtgt
     2641 tgaaggatca attcctaaat gtgatataac aacaaatgta tctacgtctt tataaatacg
     2701 catcatttcc gctgtcacac tttgtaatgg atctctaaat tcaacgcctt taatgccttc
     2761 aggtcttgtt ttcgtctttg tttctggtgt cgttacacca ataattccat aacgaatacc
     2821 attttttgtt acaatcgttg aaggcttaaa cgcgcgtttt ccatctttat aaacgttagt
     2881 acttagcatc gggaagtcta acataccctc taactttttc aactgatcgt atccaaagtc
     2941 aaattcatgg ttaccgactg ccatagcatc ataacctact gcattcattg ctttagccat
     3001 ttcttcacct ttagactggt ttgaaagtgg taaaccttgg aaggcgtctc ctgcgtctaa
     3061 cattaaatca ggcttttctt gttcttttac tgtttttaat ttagccatac cgatgacacg
     3121 ccctttttct tcggctagtc ggccatggat atcatttgta tgtaatattt tatgtgttgt
     3181 tgtttgtggt gcattagcag caaacattcg tggtgaaagt gatgctgttt tagcatttga
     3241 taatttgaac gttgctgtgt gagttggttt ttgtgttgag gcttgttgtg tatctacgtc
     3301 gcgtgtttcg tttacttttg ttgaaactac tgtagatggt ttgttagatg gtgcgttgga
     3361 tgttactttg ctgtttgtat catcagctgt tccctgcgct gatgctgata cgttggttgc
     3421 cgcttctttg ccagaatgat gagccgcttc ctttgttggc ggtacttgtt gcttatctgt
     3481 tgttgctttg tctatcgttg ttactgcatg tgctttcatt ggtgtatgct gctcagccgc
     3541 gttcgagact tgccataatc ccattacact aaaaagcaaa acgagccata cacttgtttt
     3601 aagtaataaa gctttcattt cgttccctcc attaactgtc atacaccaat atattatcat
     3661 aatccttata ttaaaatgat tgattttatg taaattattt ataaattaca tttaatatcg
     3721 aattatttta cacatttcaa tcaagccgat tcaactcaac aacagccatt tcccaccaac
     3781 aacataaaaa agcacccgat aaagtcgtct cacagcaatg cggaacttta tcggatgact
     3841 ctcatttata ttgtatatat tggcgttgga atagctttat ccgtatcaca tagcaatact
     3901 tctttttccg tatcaatatc gtgttcgttc aatacttggc caacactcat acgcgccaaa
     3961 tctttcatgt tattgtcttg tgataaatgc gataggtaaa tacgtttcgt gttacctgta
     4021 atcacgtctg tcattgcatg agccgcatcc tcattagata catgacccat atcgcctaaa
     4081 atacgttgtt tcgtcttcca tggataacga cacattctca acatatcgac gtcatgatta
     4141 ctctcaaaaa taaacgcatc gctgccacgt atcatacctt tcatacgatc agacacgtaa
     4201 cccgtatccg ttaaaatcgt aaacttctta tagttattat ggaaaatata aaattgcgga
     4261 tctattgcat catgtgacac gttaaacgat tcaacatcga aacctgcaat agattttgtt
     4321 tcataaggat taaaaatgaa tttctgatcc atagggatgc gactatcttt cttttcaatt
     4381 gcctgccaag tcttttcatt cgcataaatt ggcaattgat atttacgcgc caaaacacct
     4441 aatcctttaa tatgatcaat atgttcatgg gttactaaaa taccatttaa atcttgaata
     4501 ttacggtcaa tttgactaaa caattcttcc attttctttc cagtcaaacc aacatcaact
     4561 aatagactac ctttttcatt ttctacaaaa gtggcgttac ctgtactacc acttgctaat
     4621 acactcatgc gtatcaagcg gctcatcctt tctctttaaa cagtcaatct ctctatatga
     4681 ctgcaatata catcagtgca aatatacgat tttccctaca gaaactatca gtatattagc
     4741 tgttttgtat acctttccta ttatgtaact ttagttcatc gaccggtttc ttatatttaa
     4801 attttgataa tcacgtgcaa tgaatttctt aattattata acaatgatta gacaaatact
     4861 aaagttttta aaataacagt tacactaatt ttacaatgcg gttctactta aaatatgaat
     4921 aaatgcgcac gttggagacg caaaaacatc agccataata aaccgtacca gcaaaaaagc
     4981 tagcaccgct tctcacatat gatgagcttg caatgctagc ttattacgat tcatattaat
     5041 gattaataat tttagggtta ttatttgtcg cttcgacata gtaagtattc gttttatcct
     5101 taccgtcatg cttcacttta atttcccagt ttggttgtaa caattgaaca ttcgtttcat
     5161 tcacgactga gtagtaaccg agtctagcat taatgacttc atcacctttt ttcaagtaac
     5221 gattataata taatgcctcg attgcttttc tcacaccaat cacttgatgc ttcttatctg
     5281 caccatctgt gggcttaatg tcatccatca ttgattgttt ataactagtc gctttgttat
     5341 cttctatatt aaagttcaac atcgctttac tgttatttaa aatcggaaaa tcatcatacg
     5401 tttgttcata ttttacagaa tctgaactaa tctcgcttaa ttgatattca gcacctttaa
     5461 atacgcgctt gttaacataa tctttcaaat cttttaagtt attgtcactg acctttaccg
     5521 attgactaat gttcgcattc aataattttc caccatctga tgtggtcaaa tccgaatcgc
     5581 ctttagcttt agaactaaag tcttttgatc gccctgtaat ttgctctaat tttatacctt
     5641 taactgattt attcaatata ctagtcggta ctttaatttc ttcttgctga aaattaactt
     5701 cattgttact ctcgacttca ttaatgtgtg agcgattgac tttattaaca taaatcgata
     5761 ctaacacgat gttgacaaga ataaacacga aaatgaaaag tgtctttgtc agtttccagt
     5821 tcatttattc aagcctccca tcgttataaa cataccattc gccatcatat tctacatacc
     5881 aacgcggtac gagttcactg ttaatctgca cttcaatgtg attatgatct gaattatcct
     5941 gcatttcgta accgatagcg atgtttgtta ctttttcaaa attaatatca ctattgttcg
     6001 ctaagcttga acgtacagac tctaatttcg gcaacgattt attatcctca ctatttaaaa
     6061 caacgtcggt gcgtaataac gaacgacgat agtcaaagac gcctttttca ccccaagtga
     6121 cttgaatttg attagaacct tctttattaa acgttggata accattaagg aaacgttgat
     6181 atgttaactc gcctgactga ttattcgtac taaacaatct aaagtcttcg tttaagaaac
     6241 caccatgacc attaataaaa tcaaaggttc ctggaatcgt ttcttccatt ttgctggaac
     6301 tcgcttcatc ttcggacagg tttttataat gatatttttc atttttatcg ttatagtttg
     6361 cgacacctgt attattgttg taggttgtaa caccactctt tgaactacga acgatggttg
     6421 aatcgtcaaa tagtatagca ttcattttct caacactaat cgtgttaaat accatgcgat
     6481 atgtttttaa cttttcaggt ttacttggtg caaaaacatg cgtcgtacga tcaattgtat
     6541 ctttgtttgt gatgatatct gtgtatggtt gcatatcttt tttcactgct gctaatgcat
     6601 ctaaaaaatg atcatttttc gttgtagttg ttaattttac gtaatcgtgg cgatctttgc
     6661 ttatagcata aagcacgata ttatcatcag catcatgatc tatgaccaaa cgattgaaat
     6721 tgaaatgatt tggtactttc gcattcatgt tcagtacttg accaagatat gttgataacg
     6781 gtaaatcata cgtaaaatcg aataatataa aatcactgtt caaatcagga atcatcaagt
     6841 tatgatcacg acgaacatgt tccacggact tcacttcttt atttttcaaa ggtttcgtca
     6901 gtttattcac attagatacc gtcgcaatcg ttccttctgg atgatcattt ttcgaatgaa
     6961 taatctgaaa tggcgtaata gttgtatcca ttttggctgt catagttgtc gttaaaggtt
     7021 tcgtttcact cttcttacta tctgtattgt cgacatttgc aatatcagga gaaaagttcc
     7081 ataccatata tgtcaatacg acactcatca agacgagtag tgctaaaatg acagatttaa
     7141 tatgctcctt attattcatc ccaatcaccg tcttcaatga cttcacatgg aagtgtgata
     7201 aagatagatg taccttgacc ttctacactg tttgcccaaa tacgaccatt gtgcgcttcc
     7261 acaatctctt tcgaaatggc tagtcctaat ccagtaccac ccattttacg cgtacgtgcc
     7321 ttatctacac gatagaatcg gtcgaatatc ttatcgactt tattgatagg aataccaatg
     7381 ccattatctt taatacgaat cgtcattcga ttataaagtg gattttgttt cacgtggaac
     7441 tcgacacgtt tatcgcctct agaatatttc atcgcatttg taatgacatt atcaaatact
     7501 tgcgtcattt tatcaggatc aaattctgtg aaaatcgtct ttttcggaat atctcgaata
     7561 aatgttgtat ctttcgcaga catttcatgt cgattaataa ttttattaat gaacatgtta
     7621 aagtcgataa tttctttgtt gatttgatca gactcattat ccattttaga taactgtagc
     7681 aagtcattga ccagtcgaat cattcgttct gtttcttcac gggtaacaga taaaaattgt
     7741 ggcgcaagtt cctcatcttt ccatgcacct tcttcaagtg cttcaatgta actattcata
     7801 gaagttaaag gtgtacgtaa ctcatgtgat acattggcaa caaattcacg acgctcacgt
     7861 tcaacttgtt gttgttcagt tacgtcatgt aacacagcga tataaccagt tacaaatcct
     7921 gtttcctgca caatcgtact aaagttaaca cgtgcgatta gaccttcttc ttcatttaaa
     7981 tctaataaga aactatcatt attctcttga atttcttcca gtttaaattc atcttcaaga
     8041 cttaatacac ttaacatgta atatccgatg atgtcttctt tcgccatacc aagcatcttg
     8101 agtgccatat cattgacgat acgaatacgt ccacggcggt ctgttgcaat aataccatca
     8161 ctcatatggg tgataactga gtccagtcta cgtttctcac tttcagtatt agcctgcgct
     8221 tcttgtacac gtttagacaa gttattaaat gctaaagcta attcgccaat ttcatcatta
     8281 ccataaatct tcacacgttg cgtatagtta cctctggaca tttcgaccgt ctggttacgc
     8341 atatcggtga ttggtttggt aatcgttcgc gctataaaga atcctaggat gactgtgatt
     8401 aataatgaaa tagctgtacc aacaatgaat atttgattta tattatttaa ttggttataa
     8461 acgtcattaa tttttgattc gatataaata ttaccaatta cctttttatc gactttaact
     8521 gggatattat atacccagac acggtcctta ccaccgccat aatcttttaa aattaaatga
     8581 tcgtttgatt gtcctagtga tagtgctttt tggacagaac tatcattcgc tttttgattg
     8641 attagactac ggttagactg cttcgtcgtc gcaataataa tttggtcttt atctataaaa
     8701 cgaatttctc caatttcttg acggttggca tactcactta ataaattttg aatatctttt
     8761 tgtgcattta cggagccctt ttcgtcatat actttttcaa tactaatttc taattgtttc
     8821 gcgtactgcg taatattctt cttaaaatta tcaagcagct ctttttcaag gttatttgta
     8881 aaatataacc cgataatttg cataccaatg ataatcagta atacataaac aattacaagt
     8941 ttagtatgaa gggattgtag ttgttttagc cacttcattc gtttcgacct ctactcatgt
     9001 tgttggagga aatatccaac gcctctacgc gtcacaatat attcaggatg tgacggatca
     9061 tcttcaatct tttcacgtaa acgacgaatc gttacatcga ccgtacgtac atcgccaaag
     9121 taatcatagc cccatactgt ttgtaataaa tgttcacgtg tcattacttg tcccatatgt
     9181 tttgataaat aatggaacaa ttcaaattca cgatgtgtta attcaatatc ttcgccacgt
     9241 tttttaatag aatatgcgtc tggataaatc acaatatctt taattgtgat ttcattcgtt
     9301 acatttccag tgtcttgtgc tggttgtgag taatgacgac gtaagttcgc tttcacacgt
     9361 gcgattaatt cacgcgtact aaacggtttc gttacatagt catctgcacc tagttctaaa
     9421 ccaagcactt tatcaatttc tgaatcttta gcagtaagca ttattattgg catttcgtat
     9481 tttttgcgca cttcacgaca tacttccata ccatcacgac caggtaacat gatatctagt
     9541 aatacgatgt ctggttcttc ttcataaatt aagtcgactg catcattacc atcgtatgca
     9601 cagtacacat cgtatccttc tttttttaag ttaaattcta aaatatcagc aatcggtttt
     9661 tcatcatcaa ctacaacaac ttttctagcc atttgcataa acctcttttc ttaaatcatt
     9721 aatattattt tatcattttt tgttctttat cgtttatgtt tgtgtaaaaa atcacaggat
     9781 gtcgcttact atttttgtta ttgtgcgcat tttccgtctt ataacgtata gtaacctatt
     9841 atataattta ccatttataa gctattaatt ctattttcca atataaactt atatattact
     9901 attttttatt agtttctcac ggcggattgt gacaatgata tttaacacaa tgatgaggtc
     9961 tctattccaa taataaaatt aggataatgg caacttctag ttgcacgtca ttgctcagaa
    10021 aataaatatc attattcgtt attcgttact ttaacaattg atgttatgga ggactggtgg
    10081 catatcacaa cgaagaaata tgttgacagt agcatatgtt tgtacgtcta ataaagattt
    10141 tattgtctac gtttatcttt catatccaac cactaagtta cttgaggttc atacatttta
    10201 aagaaaatgg acgaacagat aaatcaatat gattatcatt cgtccatata tcttttatta
    10261 atataatatt aaacgcaaaa aaatgcctat cctcttggat aggcatttgt
//
LOCUS       DAXWFK010000093        10112 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.093, whole genome shotgun sequence.
ACCESSION   DAXWFK010000093 DAXWFK010000000
VERSION     DAXWFK010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10112
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.093"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(143..751)
                     /locus_tag="ACGEXS_001750"
     CDS             complement(143..751)
                     /locus_tag="ACGEXS_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFF8012750.1"
                     /translation="MDFVNNDTRQIAKNLLGVKVIYQDTTQTYTGYIVETEAYLGLND
                     RAAHGYGGKITPKVTSLYKRGGTIYAHVMHTHLLINFVTKSEGIPEGVLIRAIEPEEG
                     LSAMFRNRGKKGYEVTNGPGKWTKAFNIPRAIDGATLNDCRLSIDTKNRKYPKDIIAS
                     PRIGIPNKGDWTHKSLRYTVKGNPFVSRMRKSDCMFPEDTWK"
     gene            1150..1797
                     /locus_tag="ACGEXS_001751"
     CDS             1150..1797
                     /locus_tag="ACGEXS_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFF8012751.1"
                     /translation="MEQLPQVGFMKSFVLFWKNYVNFKGRSRRSEYWYMALWHLILMV
                     PAVCVLIINLFLFFISIAADNETLIVMSILMIILTSVYTFVYSLATFIPNLALCVRRF
                     HDISRTMLFPMIMTVFSIIFYIVLQIIDSYYDSDFTLMPMGLNILLVLLYFVYLGLTI
                     VMIVFLCFDSKPANKYGESPKYPSTIKNQPVTSETPEKACTSETPKTPEETDNQL"
     gene            2028..2927
                     /locus_tag="ACGEXS_001752"
     CDS             2028..2927
                     /locus_tag="ACGEXS_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8012752.1"
                     /translation="MGLVIEHVTKRFGKMTAVNDISLKLESGKMLGFLGRNGAGKTTT
                     FRMILGLSEPTEGHITYNGKKLDKTMYNRIGYLPEERGLHGKLTVEEELKYLATLKGM
                     SKTEIQQQISYWLERFDITENRKKRIDSLSKGNQQKIQLLASMLHKPELLILDEPFSG
                     LDPVNVELLKEAVKDLNDWGSTIVYSSHRMEHVEELCDDVCILDKGQLVVSGDINHVR
                     ASNGNKKVVIESETTLPDLTNIRGIIHSENMKQGLQLTIENEDVAKDIYQVVAHQGYV
                     KRFQVVEPSLQDIFIEKVGGKDA"
     gene            2920..4152
                     /locus_tag="ACGEXS_001753"
     CDS             2920..4152
                     /locus_tag="ACGEXS_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8012753.1"
                     /translation="MHKFWATFTLTYIKKIKAKSFVIFMIIIAALMIGLSNIDKIINM
                     FDDGPDKIGVAAPNEQIYKVFKQQANTFHSDAKFTKVSIEDAEKEVKKHKLDKAYIIK
                     VNQNRTLQGTIISEKRVSHEDSQKVQALLTAIQTNMVAGELNINKEDLQKLQAQSKVD
                     NKVISNDEVDKVSEGQKIFNYALAYGIIFLMFFIVLNYASQIAMEIASEKTSRVIEMI
                     ITSISPIQHIFAKILGVIAVAVTQIILIVLMAVICIFAFDLKDLLQGFNVEMNQLSWQ
                     IIIVGIISSIVGITAYVLLAAILGSLTSRIEDLNQSLMPLTLLGMIAFYIAIFTINNP
                     DGQLALITSYIPFLAPFQLVVRAQTSGLQIYEVILSSLISIVMVAVLIWIAIKTYKDS
                     VLTFERGLFNSMKRVFKK"
     gene            4136..4321
                     /locus_tag="ACGEXS_001754"
     CDS             4136..4321
                     /locus_tag="ACGEXS_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012754.1"
                     /translation="MYLKNKIDESDTHLSYKMMMKACYSYFFIFSYRIMSKKVYNMNV
                     KTNFLKWECEVRRNVNK"
     gene            4370..4696
                     /locus_tag="ACGEXS_001755"
     CDS             4370..4696
                     /locus_tag="ACGEXS_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFF8012755.1"
                     /translation="MLYPIFIFILAGLCEIGGGYLIWLWLREGQSSLVGLIGGAILML
                     YGVIATFQSFPSFGRVYAAYGGVFIIMSLIFAMVVDKQMPDKYDVIGAIICIVGVLVM
                     LLPSRA"
     gene            4726..5745
                     /locus_tag="ACGEXS_001756"
     CDS             4726..5745
                     /locus_tag="ACGEXS_001756"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFF8012756.1"
                     /translation="MIVEVEHQRQGVDLIKIDNDETKIIFTNYGARVVSWKYHDNNIV
                     LGNVVEADEFYHSNPFKFGASIGRYSGRIDNAKFKLKGKEYQLEKNNGEHHLHGGCHG
                     LDNKLFDYEIRNEIAQIKVIFKTVLKSADDHFPGDIDVTITHIYDADHQWSIEYEAVA
                     SEDTLFSPTNHVYFNLNRDNNVVDNHRISSNQLDMYVLDERNIVTGDILDLHEVFEDN
                     KIKLSDIFTSQHAQLSQQMTRFGGLDHPFTVGEHKMYVENHEFMLEVDTDMPHVVFFT
                     FNQPDEWDSPFNIYKPHSGFTLETQFLPNDINLYGEKAPSILKAGEVFNSKTSFRIEE
                     KVEND"
     gene            complement(5981..6634)
                     /locus_tag="ACGEXS_001757"
     CDS             complement(5981..6634)
                     /locus_tag="ACGEXS_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HFF8012757.1"
                     /translation="MISIHAISTGKIQDLPYSSKRPMRSALDKTKISQTTWLSSTGFT
                     GDEQAYKDHGGPHKAVCGFSKHNYALYQDDLPTLPTHAMFGENLTFDYLDESDVYFGN
                     QYRLGEALIEVSEIREPCWKIQAKYNIPDLVKRMSTSGKTGFYFRVLKQGYVSPNDQL
                     YLIQEAPIEHRLSVQQLNDLYYNDRQNQDMLRYALNNPFLSPTRRDKLQKMYNRTLK"
     gene            6808..7494
                     /locus_tag="ACGEXS_001758"
     CDS             6808..7494
                     /locus_tag="ACGEXS_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HFF8012758.1"
                     /translation="MKDVKALKLMTLNDVLSQINGDMTLGIGTGSTMELLLPQMAQLI
                     KERGYNITGVCTSNKIAFLAKELGIKICEINDVDHIDLAIDGADEVDPSLNIIKGGGG
                     ALFREKVIDEMASRFVVVVDETKIVQYLGETFKLPVEVDKFNWYHILRKIESYADIKV
                     ERRVNEDVAFITDNGNYILDCKLPKGIDPYKFHEYLIHLTGVFETGYFLDMADQVIVG
                     TQEGVKILEK"
     gene            complement(7560..8819)
                     /gene="sdpC"
                     /locus_tag="ACGEXS_001759"
                     /gene_synonym="lyrA"
     CDS             complement(7560..8819)
                     /gene="sdpC"
                     /locus_tag="ACGEXS_001759"
                     /gene_synonym="lyrA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic endopeptidase
                     SdpC"
                     /protein_id="HFF8012759.1"
                     /translation="MKNNKISGFQWAMTIFVFFVITMALSIMLRDFQSIIGVKHFIFE
                     VTDLAPLIAAIICILVFKYKKVQLAGLKFSISLKVIERLLLALILPLIILIIGMYSFN
                     TFADSFILLQSTGLSVPITHILIGHILMAFVVEFGFRSYLQNIVETKMNTFFASIVVG
                     LMYSVFSANTTYGTEFAAYNFLYTFSFSMILGELIRATKGRTIYIATTFHASMTFGLI
                     FLFSEEIGDLFSIKVIAISTAIVAVGYIGLSLIIRGIAYLTTRRNLEELEPNNYLDHV
                     NDDEETNHTEAEKSSSNIKDAEKTGVATASTVGVAKNDTENTVADEPSIHEGTEKTEP
                     QHHIGNQTESNHDEDHDITSESVESAESVKQAPQSDDLTNDSNEDEIEQSLKEPATYK
                     EDRRSSVVIDAEKHIEKTEEQSSDKNK"
     gene            9109..10044
                     /gene="hutG"
                     /locus_tag="ACGEXS_001760"
     CDS             9109..10044
                     /gene="hutG"
                     /locus_tag="ACGEXS_001760"
                     /EC_number="3.5.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050415 - formimidoylglutamase activity
                     [Evidence IEA];
                     GO_process: GO:0019556 - L-histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HFF8012760.1"
                     /translation="MYKQGEPNLWTGRLDSETDPKKFRHFQTVTFEDLSKLEKSSMPS
                     GVGILGYAVDKGVALNKGRIGAKEGPDAIKQAFAGLPDLNQCETLVDYGNVYHDHEEL
                     IDTQKEFAMLAAKSIANHRQTFLLGGGHDIAYAQYLATRKVYPTQSIGVINIDAHFDT
                     RAEQQSTSGTSFRQILEEDENTDYLVLGIAQGGNTQSLFDYAKEKKIDYVFADELLSH
                     VSPTIKDMIERFVHEHDVIMFTICMDVIDSAFAPGVSAPAVLGLYPHTVLELAKRIIP
                     SDKVSSVSIAEMNPTYDADNRTAKLVANLVHHFLK"
BASE COUNT         3470 a         1391 c         1885 g         3366 t
ORIGIN      
        1 agttcaataa gaggagatga atcaaaaaat atgatggata caaagaaaaa caatcaaccc
       61 atccgcatgt tgacggcacg attgattgac ttataaggag atagatttca ttttcatgat
      121 agtaatcaat gaaagatggc atttatttcc aagtatcttc gggaaacata caatctgatt
      181 tacgcatgcg agacacaaat ggattacctt tcactgtgta acgtaaagat ttatgtgtcc
      241 aatcaccttt atttggaata ccgattcgtg gactagcaat aatatcttta ggatatttac
      301 gattcttagt atcaatagac aatctacagt catttaacgt agcgccatcg atagcccgtg
      361 gaatgttaaa tgccttagtc cattttcctg ggccattcgt tacctcgtag cctttcttac
      421 ctctgttacg gaacatagcg gataaacctt cttctggttc aattgcgcgg ataagtacgc
      481 cttcaggtat accttcagat tttgttacaa aattaatgag taaatgcgta tgcatgacat
      541 gtgcataaat tgtaccacca cgtttatata atgacgtgac tttaggtgtt attttaccgc
      601 catagccatg agccgcacga tcattcaaac ctaagtaagc ttccgtttcc acgatgtagc
      661 ctgtatacgt ttgagtggta tcctgataaa tcactttgac acctaataag tttttagcaa
      721 tttgtcttgt atcattatta acgaaatcca taatattctc ctcgcaatta tatgattaat
      781 ttaaataaaa atgaaaaaga tatttttatt gtggtaaatg tttgataaaa tagtattgtt
      841 aagaattata aaaatgtaaa gtatatactt tgttatggaa tattttattt gcatagattg
      901 agactctttt aaacgtgaac attgcagtat gacatatttt tagtatagtt aacgaagcgt
      961 tgactttcaa atgagtctcc atatagatgt gtacttgtag cgggttttca ttttactagt
     1021 gatgaaatat aaagttcatt ttccaaagtg attgacgatt gaaatgaagt ggcttgataa
     1081 tgaatcttgt aaattataac ggtaacaata attaaaaata taaatatgat taacgaaagg
     1141 gagattacaa tggaacaatt acctcaagta ggttttatga aatcattcgt tttattttgg
     1201 aaaaattatg taaattttaa ggggagatca agacgtagtg aatattggta tatggcacta
     1261 tggcatttga tacttatggt tccagctgtg tgtgtgctaa taataaacct atttctattc
     1321 tttatttcta tagcggcgga taacgaaaca ttaatagtta tgagtatatt gatgattatc
     1381 cttacatctg tgtatacttt tgtctatagt ttggcaacgt ttattccaaa tttagcgttg
     1441 tgcgttcgaa gatttcacga tatatcaaga acaatgttat ttccaatgat tatgacagta
     1501 ttcagtatta tattttatat agtattacag attatcgata gttattatga cagtgatttt
     1561 acattaatgc cgatgggtct taatattttg ttagtacttc tttattttgt ttatttaggc
     1621 ctaacaattg ttatgatagt attcctatgc tttgacagta agccggcaaa taaatatggc
     1681 gaaagcccaa aatacccgtc tacaattaaa aatcaaccag tgacatctga aacaccggaa
     1741 aaagcgtgta catctgaaac accgaaaaca ccggaagaaa cagataatca attgtaatga
     1801 tataaatatc tcggcacaat gatgctggga tattttttta caaataaggc aataaaggca
     1861 tggaaaaaac atatctagtg ctaatgatac ttaacatgca aaaatggcac tatttaaatt
     1921 tttcaatttc agaaatttta aaaataaatt cattttaaaa atattgcatt tatagagaat
     1981 ggaaatgtta tattaagata aatacatatt tttttaggag gcaaagtatg ggacttgtaa
     2041 ttgaacatgt tacgaaacgt tttggcaaga tgacggcagt aaatgatatt tcattaaagt
     2101 tagaatcagg taaaatgtta ggttttctag gaagaaatgg tgcaggtaaa acaacgacat
     2161 ttcgtatgat tttaggctta agtgaaccaa ctgaaggaca cattacgtat aacggtaaaa
     2221 aactagataa aacaatgtac aatcgtattg gttatttacc ggaagaacgc ggtttacatg
     2281 ggaaattgac agttgaagaa gaattgaaat atttggcaac tttaaaggga atgtcaaaaa
     2341 cagaaataca acagcaaata tcgtattggc ttgagcgttt tgatattact gaaaaccgca
     2401 aaaaacgaat tgatagtcta tcaaaaggga atcaacaaaa aattcagctg ttagcaagta
     2461 tgttacataa accagaatta ctaatattag atgagccttt cagtggtcta gacccagtta
     2521 atgttgagtt attaaaagaa gcagttaaag acttaaatga ttggggcagt acaatcgttt
     2581 atagttctca ccgtatggag catgttgaag aattatgtga tgatgtttgt attttagata
     2641 aaggtcaact tgttgtttct ggtgatatca atcatgtacg tgcatctaat ggtaacaaga
     2701 aagttgtcat tgaatcagaa acaacactac cagatttaac aaacattaga ggcatcattc
     2761 atagtgaaaa catgaagcaa ggtcttcagt tgacgattga gaatgaagac gtagccaaag
     2821 atatttatca agttgtggca catcaaggtt atgttaaacg attccaagtt gtagaaccat
     2881 cattacaaga tatattcatt gagaaggttg gtggcaagga tgcataagtt ttgggcgaca
     2941 tttactttga catatatcaa aaaaattaaa gctaaatcat ttgtaatttt tatgattatt
     3001 attgctgcct taatgattgg cctatctaac attgataaga ttattaacat gtttgatgat
     3061 ggtccagata aaattggtgt agcagcaccg aatgaacaaa tctataaggt tttcaagcaa
     3121 caggcgaata catttcatag tgatgctaag tttactaaag tttctataga agatgctgaa
     3181 aaagaagtaa aaaaacataa acttgataaa gcttatatta tcaaggtgaa tcaaaatcgc
     3241 acgttacaag ggacaatcat tagtgagaag cgagtaagtc atgaagattc acaaaaagta
     3301 caagcactgc ttacagctat tcaaacaaat atggtagccg gggaattaaa tatcaacaaa
     3361 gaagacttac aaaaattaca agcacaaagt aaagttgata ataaggtgat ttctaatgat
     3421 gaagtcgata aagtatcaga aggacaaaag attttcaact atgctttagc ttatggcatt
     3481 atctttctaa tgttcttcat tgttttaaat tatgcatctc aaatagctat ggaaattgct
     3541 agtgagaaaa catcgcgtgt cattgaaatg attattacaa gtatttcacc aatccaacat
     3601 atttttgcta aaattttagg cgttatagca gttgccgtga ctcagattat tttaattgta
     3661 ttgatggcag ttatatgcat ttttgccttt gacttaaaag acttgctaca aggttttaac
     3721 gtggaaatga atcaattgtc ttggcaaatt attattgtcg gaattataag tagtattgtt
     3781 ggaattacgg cgtatgtatt acttgcagcc atcctcggat cattaacatc tagaatagaa
     3841 gatttaaatc aatctttgat gccactgaca ttacttggta tgattgcatt ttatatcgcg
     3901 atattcacga taaataatcc tgatggacaa cttgctttaa tcacaagtta tattccgttt
     3961 ttagcgccat ttcaattagt tgtacgcgct caaacttcag ggcttcaaat atatgaggtc
     4021 atcttaagtt ctttaatctc aattgtaatg gtagctgtct tgatatggat tgcaattaaa
     4081 acatataaag attcagtact tacatttgaa cgtggcttat ttaattcaat gaaacgtgta
     4141 tttaaaaaat aaaattgatg agagtgacac acatttatca tataaaatga tgatgaaagc
     4201 gtgttactct tattttttta tatttagcta tagaataatg tctaaaaaag tgtacaatat
     4261 gaatgtgaaa acgaactttt taaaatggga atgtgaagta agaagaaatg taaacaaatg
     4321 atatgtagcc gatagattat tgatagcaaa tgtgtgatgg agtgatttca tgttatatcc
     4381 gatttttata tttatattag ctggcttatg tgaaataggt gggggatacc tgatttggct
     4441 gtggcttagg gaaggacagt cttcacttgt tgggctaata ggcggtgcga tactcatgtt
     4501 atatggtgtc attgcgacat ttcaatcatt tccatcattc ggaagagtat atgcagcata
     4561 tggtggcgta tttatcatca tgagtttgat ttttgcgatg gtcgtagata aacaaatgcc
     4621 agataaatat gatgttatcg gtgcaatcat ttgtattgta ggtgttttag taatgttgct
     4681 acccagcaga gcttgaacac ttgtggttag aagggagaag taaaaatgat agttgaagtt
     4741 gagcaccaaa gacaaggtgt agatttaatc aaaattgata atgatgaaac taaaattatt
     4801 tttacgaatt atggagcaag agtagtgtct tggaaatatc atgataataa tattgttctt
     4861 ggaaatgttg ttgaagcgga tgaattttat catagtaatc cttttaaatt tggtgcttct
     4921 ataggaagat atagcggtag aattgataat gctaaattta agttgaaagg taaagaatat
     4981 cagttagaaa aaaacaacgg tgaacatcat ttacatggtg gatgtcatgg tttggacaat
     5041 aagttatttg attatgaaat tagaaatgaa attgctcaaa ttaaagttat atttaagacg
     5101 gtgttgaaat cagctgatga tcatttcccg ggtgatatcg atgtgacgat tacacatatt
     5161 tatgatgcgg atcaccaatg gtcgattgaa tacgaagcag ttgcatcaga agacacattg
     5221 tttagtccta cgaatcatgt gtacttcaat ttgaatcgag ataataatgt cgttgataat
     5281 catcgcattt caagtaatca attagatatg tatgtattag atgagcgaaa tattgttaca
     5341 ggtgatattc tagatttaca tgaagtgttt gaagataaca agataaaatt gagtgatatt
     5401 tttacatcac aacatgcgca attaagccaa caaatgacac gttttggtgg tctcgatcac
     5461 ccatttacag ttggtgaaca taaaatgtat gttgaaaatc atgaatttat gttagaggtc
     5521 gatacagata tgcctcatgt tgtgttcttt acttttaacc agcctgatga gtgggatagt
     5581 ccatttaata tttataaacc acattctggc tttacattag aaacacaatt tttaccaaat
     5641 gatattaatt tatatggtga aaaagcaccg tcaattttaa aagctggtga agtgttcaat
     5701 tcgaaaacaa gtttccgtat cgaagaaaaa gtagaaaatg attaaaagta tcggcattta
     5761 tataatattt atgttatttg gcggaatttt gaataggtta tcaagaatcg tatcaaatga
     5821 agtgacatca ttaatataca gtttgaaata aagaaaatat ataacataaa aaaggaatca
     5881 atagcatcag tggttataga taagactatt atacaaacga tgtgtaagtt gattcctttt
     5941 tgttttgaaa agttcattta acacttataa tgaaaggtaa ttatttcaat gttctgttat
     6001 acattttttg aagtttatcg cgtcttgttg gtgacagaaa tggattgttt agtgcatatc
     6061 gtaacatatc ttgattttgt ctatcattat aataaaggtc attaagctgt tgtacagata
     6121 aacgatgttc gattggtgct tcttgtatta agtaaagctg atcatttgga gatacatagc
     6181 cttgttttaa tacccggaaa tagaaacctg ttttaccaga tgtagacatg cgcttcacta
     6241 aatcaggaat attatatttt gcttgaattt tccagcatgg ttctctaatt tcagaaacct
     6301 caattaacgc ttcacctaaa cgatattgat taccaaagta aacatcagat tcgtctaaat
     6361 aatcaaatgt taaattctct ccaaacatcg catgagtagg tagtgtaggt aaatcatctt
     6421 gatacagtgc ataattatgc ttactaaacc cacaaactgc tttatgtggt ccaccatgat
     6481 ctttataagc ctgttcatca ccagtgaaac cagttgaaga taaccatgtt gtttgtgaaa
     6541 tcttagtttt atctaaagca gagcgcatcg gtcttttcga gctataaggc aaatcttgga
     6601 tttttcctgt cgaaattgcg tgaattgata tcatatcatt ctccttaacg agtattattt
     6661 aaaatatatt gtactataaa acatttttag aattgtaaat tctgattgat atgtttaaaa
     6721 aaatgttaga aataagggta tgttaattta tacgagtcat gtaaaatatg aattgaatag
     6781 attacataaa tatgggaggc taccttcatg aaagatgtca aagcactcaa gttaatgaca
     6841 ttaaatgatg ttttaagtca aatcaatggt gacatgacgc tgggaattgg tacaggaagt
     6901 acaatggaat tactcttacc tcaaatggcg caactaatta aagaacgtgg ttacaatatt
     6961 acaggtgtat gcacttctaa taaaattgca tttttagcaa aagaattagg tattaaaata
     7021 tgtgaaatca atgatgttga tcatatcgac ttagcaattg atggtgctga tgaagtagat
     7081 ccatctttaa atataattaa aggtggcggt ggtgcgctgt tcagagagaa agttatagat
     7141 gaaatggcat cgcgttttgt tgtggttgtc gatgaaacga aaatagttca atacttaggt
     7201 gagacgttta agttaccagt agaggtggat aaatttaact ggtatcacat attacgtaaa
     7261 attgaatcat acgctgatat aaaagtagaa cgtcgtgtaa acgaagatgt cgcatttata
     7321 actgataatg gcaattatat tttagattgt aagttaccaa aaggaattga cccatataag
     7381 ttccatgagt atttaattca tctgacaggt gtgtttgaga caggatactt cttagatatg
     7441 gcagatcaag ttattgtagg cactcaagag ggtgttaaaa ttttagaaaa ataatatata
     7501 gtatagaaaa gcgcttgcta gcatcatctt tttaagaggt actagcaagc gctttgttat
     7561 tatttgtttt tatctgaaga ttgttcttca gttttttcga tatgtttttc tgcatcaatt
     7621 acaactgatg aacgtctgtc ttctttataa gtcgcaggtt cttttaatga ttgctctatt
     7681 tcatcttcat ttgaatcgtt tgttaaatcg tcactttgtg gtgcttgttt aactgattct
     7741 gctgattcta ctgactccga agtgatgtca tgatcttcat catgattaga ttcagtttga
     7801 ttacctatgt gatgttgagg ttctgttttt tcagtacctt catgaatgct tggttcgtca
     7861 gccactgtat tttcagtatc atttttagca acaccaaccg ttgatgcagt agctacacct
     7921 gttttttcag catctttaat atttgaagaa gatttttcag cctcagtatg attagtttct
     7981 tcatcgtcat tgacatggtc taaataatta ttaggctcaa gttcttcaag gtttcgtctt
     8041 gttgttaaat atgcaatacc tcggataatt aagcttaaac caatgtatcc tactgcaacg
     8101 attgctgttg aaatggcgat gactttgatt gaaaatagat cgccgatttc ttcgctaaac
     8161 aagaaaataa gtccgaatgt cattgaagca tggaatgtcg ttgcaatata aattgtacgt
     8221 cctttagtcg ctctaattaa ttcaccaaga atcatagaga atgagaatgt ataaaggaag
     8281 ttataagcag caaattctgt accataagtt gtatttgctg agaaaactga atacatcaaa
     8341 ccaacaacaa tacttgcaaa gaacgtgttc attttcgttt caacaatatt ttgtaagtat
     8401 gaacggaatc cgaattctac tacgaacgcc atcagaatat gtccaatcag aatgtgtgta
     8461 ataggtactg ataagcctgt tgattgtaat aaaataaagc tatctgcaaa tgtattaaag
     8521 ctgtacatac caataattag aataattaaa ggtaaaatta aagctagcaa tagacgttca
     8581 attactttca ggctgattga gaattttaaa cctgcaagtt ggaccttttt atatttgaaa
     8641 acgagtatac aaatgattgc agcaattaat ggtgctagat ctgtaacttc aaatataaag
     8701 tgtttgacac caattataga ctggaaatct ctgagcataa tggataacgc cattgtaatg
     8761 acaaagaaga cgaaaatcgt cattgcccat tgaaaaccag aaattttatt gttcttcatt
     8821 atatgtaacc tccattaggt aacaagcaaa atatctttta gtaacaatta tacatattac
     8881 aagccgagag ataaattgtc tatgtcaata ttaatgaaaa tgtaatagtc ttgacgtatt
     8941 ttattaacat aaatcagcta agtattcgtt attacataga aaataggggt aaaagtaatg
     9001 tagataagta tttttaagat acggatttaa ctatgaattg aattcaaaat ttcctatacg
     9061 ttatacttta attgttaata agccacataa ataagagggg gaaatgctgt gtacaagcaa
     9121 ggtgaaccaa atttatggac tggaaggtta gatagtgaaa cagacccgaa aaaatttaga
     9181 cattttcaaa cagtaacatt tgaagatttg tctaagctgg aaaagagtag tatgccatca
     9241 ggggtcggta tattaggcta tgctgttgac aaaggtgttg ctttaaacaa ggggcgcatt
     9301 ggtgcaaaag aaggaccaga tgcgattaaa caagcatttg caggtttgcc ggatttgaat
     9361 caatgtgaaa ctttagtcga ttacggaaat gtttatcatg atcatgagga attaattgat
     9421 actcaaaaag aatttgctat gcttgcagcg aagtcaattg ctaatcatag acaaacattt
     9481 ttattaggtg gtggacatga tattgcgtat gctcaatatt tagcaacacg taaagtctat
     9541 ccaacacaat ctattggtgt gataaatatt gatgcgcatt ttgatacacg tgctgaacaa
     9601 caatctacat ctggaacgag ctttagacaa attttagaag aagatgaaaa cacagattat
     9661 ttagtgcttg gtattgctca aggtggtaat acgcaaagtt tatttgatta tgctaaagag
     9721 aaaaagattg attatgtctt tgcagatgaa ttattgagtc acgtatcacc aacaattaaa
     9781 gatatgattg aacgttttgt acatgaacat gatgtcatta tgtttacgat ttgtatggat
     9841 gtcattgata gtgcgtttgc gcctggggta agtgcgccgg cagtgttagg tttgtatcca
     9901 catactgttc ttgaattagc aaaacgtatt attccaagtg ataaggtgtc ttcagttagt
     9961 attgctgaga tgaatccaac atatgacgct gacaatagaa ctgctaagct cgttgctaat
    10021 ttagtgcatc attttttaaa ataattaaaa atatttatat gtgacgagat ttgcattgca
    10081 gggcagaatg tgagtatttc ttaatagaag tt
//
LOCUS       DAXWFK010000094        10100 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.094, whole genome shotgun sequence.
ACCESSION   DAXWFK010000094 DAXWFK010000000
VERSION     DAXWFK010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10100
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.094"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            67..1209
                     /locus_tag="ACGEXS_001761"
     CDS             67..1209
                     /locus_tag="ACGEXS_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFF8012761.1"
                     /translation="MEIYADYAATTPVKPEVVDAMMTIYNSHYGNPSSIHAKGRDARK
                     YLDESRRQIAQLLGADTHEIIFTSGATESNNTAIKGIVKANEQLGNHIITSKIEHHSV
                     LHVFEQLEREGFDVTYLDVDDTGAIDLDQLEETITDKTILVSIMFVNNEVGTVQQIYD
                     IQDIIAETNAYFHVDAVQAIGHLDVKFDEFEIDAMSITAHKFGGPKGVGALLVKDHVT
                     LDYPQLGGEQELKRRAGTENLAQIVGMAKALQLAEKNRDDNNIHLMNLKEQFLVKLQE
                     RAIPFELNGSMTDATGHIVNLYFPFVEVETMLTLLDMAQIYVSSGSACTAGSTQPSHV
                     LDAMFEDEERSNHSIRFSFNELTTENEINAIVAEIHKIYFKFKEES"
     gene            1210..2328
                     /gene="mnmA"
                     /locus_tag="ACGEXS_001762"
     CDS             1210..2328
                     /gene="mnmA"
                     /locus_tag="ACGEXS_001762"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFF8012762.1"
                     /translation="MSNKDIRVVVGMSGGVDSSVTAHVLKEQGYDVIGIFMKNWDDTD
                     ENGVCTATEDYNDVIEVCNQIGIPYYAVNFEKEYWDKVFTYFLDEYKKGRTPNPDVMC
                     NKEIKFKAFLDHAMNLGADYVATGHYARIHRHEDGHVEMLRGVDNNKDQTYFLNQLSQ
                     QQLSKVMFPIGDIEKSEVRRIAEEQGLVTAKKKDSTGICFIGEKNFKTFLSQYLPAQP
                     GDMITLDGKKMGKHSGLMYYTIGQRHGLGIGGDGDPWFVVGKNLKDNVLYVEQGFHHD
                     ALYSDYLIASDYSFVNPEDNDLDQGFECTAKFRYRQKDTKVFVKRENDHALRVTFAEP
                     VRAITPGQAVVFYQGDVCLGGATIDDVFKNEGQLNYVV"
     gene            2790..3458
                     /locus_tag="ACGEXS_001763"
     CDS             2790..3458
                     /locus_tag="ACGEXS_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFF8012763.1"
                     /translation="MIDQQTIYQYIQNGKIEEALQALFGNIEENPTIIENYINAGIVL
                     ADANEIEKAERFFQKALTIDPKNGVVFFNLANVYYNQQRYQEAIKLYQQALQTEIEQV
                     DCNYMIGMAFNQLESFKLALPYLMTAAELDKDKDAEVQFQYGLVLCQLEMFNEAITQL
                     KHVLTIDKNHVDARYNLGLALFMKNEDIDEAITHFKEAVTIDPKHLLSQHALKTFTKM
                     KEEE"
     gene            3460..5937
                     /locus_tag="ACGEXS_001764"
     CDS             3460..5937
                     /locus_tag="ACGEXS_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFF8012764.1"
                     /translation="MSDPTLFDYSMIKGTVEAILFQNSDNFYTVLKVDTIETNEDFDT
                     MPTVVGFLPNIVEGDVYTFKGQVVDHPRYGKQLKAETFEKEMPQTKEAIISYLSSDLF
                     KGVGKKTAQNIVNTLGDNAINDILDDHSVLEKVSGLSKKKQKQIAEQISANQESEKIM
                     IRLHDLGFGPKLSMAIYQFYLGDTLTILDRNPYQLIYDIKGIGFNKADQLARNIGIAY
                     NDNERLKAALLYTLEEECIKQGHTYLPINVVIDLTVDVLNYQDEEVIEPEKLDEMLQY
                     LNEEKRLIIDNEQVAIPSLYYSEIKSVQNLFRIKTHTNKLTEIEQSDLQMHIGEIEDA
                     NQVNYAASQKEALQTAINSKVMLLTGGPGTGKTTVIKGIVELYAEIHGLSLDYDDYVN
                     DDYPVVLAAPTGRASKRLQESTGLEAMTIHRLIGWNQDTKPEDILENEINARLIIIDE
                     MSMVDTWLFHQFLSAVPLDAQLIFVGDEDQLPSVGPGQVFKDLIESKAIPRVNLTEVY
                     RQQDGSSIIELAHRMKLGQKIDITQRFHDRSFINCQANQIPTVVEKVVTSAVNKGYTM
                     ADIQVLAPMYKGNAGIKRLNQVLQDILNPKKKDTREIEFGDVVFRKGDKVLQLVNRPN
                     DNIFNGDIGVIVGIFWAKENALNKDVLVVDFEGNEITFTKQDMMELTHAYCTSIHKSQ
                     GSEFPIVIMPIVKQYFRMLQRPILYTGLTRAKTSLVLLGDPEAFDIGLKTNGQARLTQ
                     LCTLLKNYFNSEDEEMLENTATNDTGASQTTIDDQVEAPMSSNDSEEVMSDSTKTDTN
                     VLTEATMFKIDPMINMGEITPYDFIER"
     gene            6280..8910
                     /gene="alaS"
                     /locus_tag="ACGEXS_001765"
     CDS             6280..8910
                     /gene="alaS"
                     /locus_tag="ACGEXS_001765"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFF8012765.1"
                     /translation="MKKLKASEIRQKYLDFFVEKGHMVEPSAPLVPIDDDTLLWINSG
                     VATLKKYFDGRETPKKPRIVNSQKAIRTNDIENVGFTARHHTFFEMLGNFSIGDYFKQ
                     EAIEFAWEFLTSDKWMGMEPDKLYVTIHPEDMEAYNIWHKDIGLEESRIIRIEGNFWD
                     IGEGPSGPNTEIFYDRGEAYGQDDPAEEMYPGGENERYLEVWNLVFSEFNHNKDHSYT
                     PLPNKNIDTGMGLERMASVSQNVRTNYETDLFMPIMNEIEKVSGKQYLVNNEQDVAFK
                     VIADHIRTIAFAISDGALPANEGRGYVLRRLLRRAVRFSQTLGINEPFMYKLVDIVAD
                     IMEPYYPNVKEKADFIKRVIKSEEERFHETLEDGLAILNELIKKAKATTNEINGKDAF
                     KLYDTYGFPIELTEEIAVQAGLKVDMTTFESEMQQQRDRARQARQNSQSMQVQSEVLK
                     NITSASTFVGYDTATAQTTLTHLIYNGEEVSQVEAGETVYFMLTETPFYAISGGQVAD
                     TGIVYNDNFEIAVSEVTKAPNGQNLHKGVVQFGQVNVGATVSAEVNQNDRRDIQKNHS
                     ATHLLHAALKSVLGDHVNQAGSLVEADRLRFDFSHFGPMTNDEIDQVERLVNEEIWKG
                     IDVNIQEMDIASAKEMGAMALFGEKYGDVVRVVNMAPFSIELCGGIHVRNTSEIGLFK
                     IVSESGTGAGVRRIEALTGKAAFLYLEDIQEKFNTMKSQLKVKSDDQVVDKLTQLQDE
                     EKALLKQLEQRDKEITSLKMGNIEDQVEEINGYKVLVTEVDVPNAKAIRSTMDDFKSK
                     LQDTIIILASNVDDKVSMVATVPKSLTNNVKAGDLIKQMAPIVGGKGGGRPDMAQGGG
                     TQPENISKSLSFIKDYIKNL"
     gene            8973..9233
                     /locus_tag="ACGEXS_001766"
     CDS             8973..9233
                     /locus_tag="ACGEXS_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFF8012766.1"
                     /translation="MENFDKTMKFDYEELPTQDVRDVLNNVYRTLDERGYNAVNQIVG
                     YLLSGDPAYIPRQNEARNQIRHIDRDVIMEELVSYYLKEQNK"
     gene            9237..9665
                     /gene="ruvX"
                     /locus_tag="ACGEXS_001767"
     CDS             9237..9665
                     /gene="ruvX"
                     /locus_tag="ACGEXS_001767"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFF8012767.1"
                     /translation="MLQHKILGLDVGSRTVGIAISDIMGWTAQGLDTLRINEENKELG
                     IDQLVDIIKKHNVGTVVIGLPKNMNNSIGFRGEASLTYKEKLLEAYPSIEIVMWDERL
                     STMAAERSLLEADVSRQKRKQVIDKMAAVFILQGYLDSLH"
     gene            9680..9988
                     /locus_tag="ACGEXS_001768"
     CDS             9680..9988
                     /locus_tag="ACGEXS_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFF8012768.1"
                     /translation="MTEHNHDSQLEINNEEELLTLFDEEGNEVLYRKVLEFYHPEFKK
                     EYVILAEEGAQSDEDDMIELVPMINEPDESGDGGKLVPIETDEEWDMIEEVVNTEMEE
                     "
BASE COUNT         3695 a         1375 c         2004 g         3026 t
ORIGIN      
        1 agtagaaaaa tagtatgtaa ggtgctatta ttggaacatt gataaaattg tgaggagtgt
       61 ccaaacatgg aaatttatgc agattatgct gcgaccacac cagtaaaacc tgaagtagta
      121 gatgcgatga tgacgattta caattcgcat tatggtaatc catcatcaat acatgctaag
      181 ggaagagatg cgcgtaaata tttagatgag tcaagacgtc aaattgcaca attacttggt
      241 gcagatacac atgaaattat atttacgagt ggcgcaacag aatcgaataa tacggcaatt
      301 aaaggcatag taaaagcaaa tgaacaattg ggtaatcaca ttatcacatc gaagattgaa
      361 catcattctg tgttacatgt ttttgaacaa ttagagcgag aaggttttga tgtaacatat
      421 ctagatgttg atgatacagg tgctattgat ttagatcaac ttgaagaaac aattacagat
      481 aagacgattt tagtatctat tatgtttgta aataatgaag ttggcacagt gcaacaaatt
      541 tatgatattc aagatattat tgctgagaca aatgcatatt tccatgttga tgcggttcaa
      601 gcaattggac atcttgatgt taagtttgac gaatttgaga tcgacgcaat gagtattact
      661 gctcataagt ttggcggacc aaaaggtgtc ggtgcattac tagttaaaga ccatgttaca
      721 ctagattatc ctcaacttgg cggtgaacaa gaattgaaac gccgtgcagg cactgaaaat
      781 cttgcgcaaa ttgttgggat ggctaaagca ttacaattag cagaaaagaa tcgagacgat
      841 aacaatattc atttaatgaa tttaaaagaa caatttttag ttaaattaca agaaagagcg
      901 attccatttg aattaaatgg ttcaatgacg gatgctacag gccatattgt aaatctatat
      961 tttccgtttg ttgaagttga aacgatgtta acattgttgg atatggcaca aatctatgtg
     1021 tcgtctggat ctgcatgtac agcaggctct acacaaccgt cgcatgtttt agatgcaatg
     1081 ttcgaagatg aggagcgatc aaatcattcg attcgattta gttttaacga attgactact
     1141 gaaaatgaaa ttaatgcaat tgtagctgaa attcataaaa tatattttaa atttaaggag
     1201 gagtcataat tgtcaaataa agatatacgt gttgtcgttg gtatgtcagg cggtgtagat
     1261 agttctgtaa cagcccacgt cttaaaagaa caaggttatg atgtcattgg catatttatg
     1321 aaaaactggg atgacactga cgaaaatggc gtatgtactg caactgaaga ttacaacgat
     1381 gttattgaag tgtgtaatca aattggcatt ccgtattacg ctgttaattt tgaaaaagaa
     1441 tattgggata aagtctttac gtatttctta gatgaataca aaaaaggtcg tactccaaat
     1501 ccagacgtta tgtgtaataa agaaattaag tttaaagcct ttttagatca tgcgatgaat
     1561 ttaggtgcag attatgtagc aacaggacat tacgcacgca tacatcgtca tgaagatggt
     1621 catgttgaaa tgttacgtgg tgtagataat aataaagatc aaacatactt cttgaatcaa
     1681 ttatctcaac aacaactttc aaaagtgatg ttcccaattg gcgacatcga aaagagtgaa
     1741 gtgcgtcgaa ttgctgaaga acaaggactt gttactgcta agaaaaaaga ttctacaggc
     1801 atttgtttta tcggcgaaaa aaactttaaa acatttttat cacaatattt acctgcacaa
     1861 ccgggtgata tgataacact tgatggtaag aaaatgggta aacatagtgg tttgatgtat
     1921 tacacaatag gacaaagaca tggattaggt ataggtggag atggcgatcc ttggtttgtt
     1981 gtcggtaaaa acctaaaaga taatgtttta tatgtagaac aaggattcca tcacgatgca
     2041 ttatacagtg attacttaat tgcttcagac tattcatttg taaatccaga agataatgac
     2101 ttagatcaag gttttgaatg tacagctaaa tttagatatc gccaaaaaga tacgaaagtt
     2161 tttgtgaaac gtgaaaacga ccatgcacta cgtgttactt ttgctgagcc agtaagagca
     2221 atcacacctg gacaagcagt tgttttttat caaggtgatg tgtgtcttgg tggtgcaaca
     2281 attgacgatg tgttcaaaaa tgaaggtcaa ttaaattatg ttgtataaac aatggcaaca
     2341 ataaattact tatttgaagt ttcaacgttg aaaatgacga aagacagttt ttgatgagaa
     2401 taattcatga ggatagagtc tgggacatca caatgtccta ggctctacaa tgttatattg
     2461 gcgggaccac aacatagaga atttcgtaaa gaaattctac aggcaatgcc agttggggat
     2521 aacgaattta attttgttaa aatatcattt ctgtcccact ccctatgcat gaatctaatt
     2581 atgtattctt atttttaagt acataatagt ggtggctaat gtggaagaac cattacataa
     2641 taaaccgtta atggttctta agcatttcta ttccattccc gctttttcat gaatgaagat
     2701 gatattagat tatattttat tcgttgttaa gtgattcgag acatacaatt tatcaagatg
     2761 ttataattga tgagaaatga ggttcgtaaa tgatagatca acaaacaatt tatcaataca
     2821 tacaaaatgg aaaaatagaa gaagcgttac aagcattgtt cggaaatatc gaagaaaatc
     2881 ctacaattat tgaaaattat attaatgctg gtatcgtact tgctgatgcg aatgagattg
     2941 aaaaggcaga gcgttttttc caaaaagctt taacaataga tccgaagaat ggcgtcgtat
     3001 tttttaatct agcaaatgta tattataatc agcaacgtta tcaagaagct attaaattat
     3061 atcaacaagc attacaaaca gagattgaac aagttgattg taattatatg atcggtatgg
     3121 cgtttaatca gttagaatca tttaagctgg cattgccgta tttaatgact gctgcggaac
     3181 tagataaaga caaagatgca gaagttcaat ttcaatatgg tcttgtatta tgtcaattag
     3241 aaatgtttaa tgaagccata actcaactta aacatgtatt aacgattgat aaaaatcatg
     3301 ttgatgcaag atacaatttg ggcttagcgt tatttatgaa aaatgaagat attgatgaag
     3361 caataactca ttttaaagaa gctgtgacta tcgaccctaa acacttatta agtcagcatg
     3421 cgctgaaaac attcactaaa atgaaagagg aggagtaaca tgtcagaccc tacactattt
     3481 gattattcaa tgatcaaagg tactgttgaa gcgatattgt ttcaaaacag tgataatttt
     3541 tatacagtgc ttaaagtaga tactattgaa acaaatgaag actttgatac aatgccaact
     3601 gttgtagggt ttcttccgaa tattgttgaa ggcgatgtct acacatttaa agggcaagtt
     3661 gttgatcatc cacgttatgg taaacaatta aaagcagaga cttttgaaaa agaaatgcca
     3721 caaacgaaag aagcaattat aagttattta tctagtgatt tgtttaaagg tgtaggtaaa
     3781 aagacagctc aaaatattgt taatacatta ggtgacaatg cgataaatga tatattagat
     3841 gaccattcag tgctagaaaa agtttcggga ttatctaaaa agaaacagaa acaaattgca
     3901 gaacaaattt cagcaaatca agaatctgaa aaaataatga ttcgtttaca tgatttaggg
     3961 tttggtccta aactttcaat ggcaatttat cagttttatc tcggcgatac actgactatt
     4021 ttagatcgaa atccatatca attgatttat gatattaaag gtattggttt taataaagca
     4081 gatcaacttg ctagaaatat tggtatcgct tataacgata atgaacgatt aaaagctgcg
     4141 ttgctctata cgttagaaga agaatgtatt aaacagggac atacatattt gcctatcaat
     4201 gttgttatag atttaacggt tgatgtatta aattatcaag atgaagaggt catcgaacct
     4261 gaaaaattag acgaaatgtt gcaatactta aatgaggaaa aacgactaat tattgacaat
     4321 gaacaagttg caattccaag tttgtattat tctgaaatca aaagtgttca aaacttattc
     4381 agaataaaga cgcatacaaa taaacttaca gaaattgaac agtcagattt acaaatgcat
     4441 attggtgaaa tagaagatgc gaatcaagtg aactatgcag cttcacaaaa agaagctttg
     4501 caaacggcta tcaattctaa ggtgatgcta ttaaccggtg gaccgggaac aggaaaaacg
     4561 acagtaatta aaggtattgt tgaattatat gctgagattc atggcttatc attagattat
     4621 gatgattatg tcaatgatga ttatccagtt gtattagcag cgccaacagg aagagcgtct
     4681 aagagactac aagagtcaac tggattagaa gcaatgacga ttcatcgttt aataggttgg
     4741 aatcaagaca caaagccaga ggatatatta gaaaatgaaa taaacgcacg actcataatc
     4801 attgatgaga tgtctatggt agatacttgg ttgttccacc aatttttaag tgcagttcct
     4861 ttagatgcac aattgatatt tgtaggtgat gaggaccaat tgccttctgt aggtcctggt
     4921 caagtattta aagatttaat tgaatcgaaa gctataccac gagtaaatct gactgaagta
     4981 tatcgtcaac aagatggttc gagcattata gaattagcac atcgaatgaa gttgggtcag
     5041 aaaattgata ttacacagcg ttttcatgat agaagtttta ttaactgtca ggcaaaccaa
     5101 ataccaacag ttgttgagaa ggtagttact agtgctgtta ataaagggta tactatggct
     5161 gatattcaag tgcttgcacc aatgtataaa ggaaatgccg gtattaaacg tttaaatcaa
     5221 gttttacaag atattttaaa ccctaaaaag aaagacacac gagaaattga gtttggtgat
     5281 gtagtcttta gaaaaggtga caaagtatta caacttgtta ataggccaaa tgataacata
     5341 ttcaatggtg acataggagt tattgttggt attttttggg cgaaagaaaa tgcccttaat
     5401 aaagatgtac ttgttgtgga ttttgaaggt aatgaaatta catttacgaa acaagatatg
     5461 atggaattaa cacatgctta ttgtacatca atccataaat cgcaaggttc agaatttcct
     5521 attgttatca tgcctattgt taaacagtat tttagaatgt tacaaagacc gatattatat
     5581 actggtttaa ctagagcgaa aacgagttta gttttacttg gtgatccgga agcatttgat
     5641 attggattga aaacaaatgg tcaagcgcgt ttaacacaat tatgtacatt gttgaaaaat
     5701 tattttaatt cagaagatga agaaatgctg gaaaacacag caactaatga tacaggagct
     5761 tcacaaacga caatagatga tcaagtagaa gcgccaatgt catcgaatga tagtgaagaa
     5821 gttatgtctg attcaacaaa aacagataca aatgtattaa cggaagctac aatgtttaaa
     5881 attgacccaa tgattaatat gggagaaatc acgccgtatg actttataga acgttgacaa
     5941 caagcgtaaa tgtaactaaa atagaattaa attcgtataa agatgagtag ttaatgattt
     6001 gtttatcaga gatgtactgg ttggtgaaaa gtacaacaaa cattagtgaa cgctacttta
     6061 tgcacaactg tatagtcgaa ttaaagtgat aaatgtgttg aattgcattt ataactaggg
     6121 tggtaccgcg ataacgttcg tcccttttga ggatgggcgt tttttatttt ttaataataa
     6181 aaaatgatat tgatttatac tagttttgca aatcgactat ctgtgtcatc aatttttatg
     6241 gaatggtaat ttaaaaacag taaataggat gtgtaaatga tgaaaaagtt aaaagcgagt
     6301 gaaattagac aaaaatatct agatttcttt gttgaaaaag gacatatggt tgaaccttct
     6361 gcaccattag tgccaattga tgatgataca ttattatgga ttaattcagg tgtagcaaca
     6421 ttaaagaaat attttgatgg acgtgaaaca cctaaaaagc caagaattgt aaactctcaa
     6481 aaagctattc gtacaaatga tattgaaaat gttggtttca cagcgcgtca ccatacattc
     6541 tttgaaatgt taggtaactt ctcaattggt gattatttta aacaagaagc gattgaattt
     6601 gcttgggaat ttttaacgag tgataaatgg atgggtatgg agccagataa attgtacgtt
     6661 acgattcatc cggaagatat ggaagcatac aacatttggc ataaagatat tgggcttgaa
     6721 gaaagtcgta ttattcgcat tgaaggtaac ttctgggata ttggtgaagg gccttcagga
     6781 ccgaacactg agattttcta tgatcgcgga gaagcatatg gacaagacga tccggcagaa
     6841 gaaatgtatc caggtggaga aaatgaacgc tatcttgaag tatggaactt agtatttagt
     6901 gaattcaatc ataataaaga tcatagttac acaccattac ctaataaaaa tattgatact
     6961 ggcatggggc ttgagcgtat ggcctcagtt tctcaaaatg tacgtactaa ctatgaaaca
     7021 gatttattta tgcctataat gaatgaaatc gaaaaagtat caggtaaaca atatttagta
     7081 aacaacgaac aagatgtggc atttaaagta attgctgacc acattcgtac gattgcattt
     7141 gcaatttctg atggtgcatt acctgccaat gaaggtagag ggtatgtatt acgtcgattg
     7201 ttacgtcgtg ccgttcgttt tagtcaaacg ttaggaatca atgagccatt tatgtacaaa
     7261 cttgttgata ttgttgcaga cattatggaa ccatattatc caaatgttaa ggaaaaagca
     7321 gatttcatta agcgtgttat aaagtctgaa gaagaacgat tccatgaaac attagaagat
     7381 ggtttagcga ttttaaatga attaattaaa aaagctaaag cgacaacaaa tgaaattaat
     7441 gggaaagatg catttaaatt gtatgatacg tatgggttcc caattgaatt aactgaagaa
     7501 atagcagtgc aagcaggatt gaaagttgat atgacaacat tcgagtcaga aatgcaacaa
     7561 caacgtgatc gtgcacgtca agcacgtcaa aattctcaat caatgcaagt tcaaagtgaa
     7621 gtattgaaaa atattacatc tgcaagtact tttgttggtt atgatactgc gacagctcaa
     7681 acaacactaa cacacttgat atacaatggt gaagaagttt cacaagttga agcgggtgaa
     7741 acagtatact tcatgttaac ggaaacacca ttttatgcaa tcagtggtgg acaagttgcg
     7801 gatacaggta ttgtttataa tgacaatttt gaaattgctg ttagtgaagt aaccaaagca
     7861 ccaaatggtc aaaacttgca taaaggagta gtacaatttg gccaagtaaa tgttggcgct
     7921 acagtgtctg ctgaagtgaa ccaaaatgat cgacgtgaca ttcaaaagaa ccatagtgca
     7981 acacatttat tacatgcagc gttgaaatca gtactgggtg atcatgttaa ccaagctggt
     8041 tcactagtag aagcagatcg tttacgtttt gatttctctc attttggtcc aatgactaat
     8101 gatgaaattg atcaagttga acgcttagta aatgaagaaa tttggaaagg tattgacgtt
     8161 aacattcaag aaatggatat tgcttcagct aaagaaatgg gcgcaatggc attattcggt
     8221 gaaaaatatg gtgatgttgt gcgtgtagta aatatggcac cattttcaat tgaattatgt
     8281 ggtggtattc atgtccgcaa tacttctgaa attggcttat tcaaaatagt aagtgagtca
     8341 ggtacaggag ctggtgtgcg tcgtattgaa gcattaacag gtaaagcagc tttcttatat
     8401 ttagaagata ttcaagagaa atttaatacg atgaaatcac agctgaaagt gaaatctgat
     8461 gatcaagtag tcgataagtt aacacaatta caagatgaag aaaaagcatt attaaaacaa
     8521 ttagagcaac gtgacaaaga aatcacatca cttaaaatgg gtaatattga agatcaagtt
     8581 gaagaaatca atggctataa agtattggtt actgaagtgg atgtaccaaa tgcgaaagca
     8641 attcgctcga caatggacga ttttaaatct aaactacaag atacaattat cattcttgca
     8701 agtaatgttg atgataaagt atcgatggtt gcaactgtcc ctaaatcttt aacaaataac
     8761 gttaaagccg gtgatcttat caaacaaatg gcaccaatcg ttggtggtaa aggtggcggt
     8821 cgtccagata tggctcaagg tggcggtaca caacctgaaa atatctcaaa atcattaagc
     8881 tttattaaag attacattaa aaatctataa tttaaagcca taatattgta tgatgatagt
     8941 gagttatatt tgtaattaaa ggagtgtcgc aaatggaaaa ctttgataaa acaatgaaat
     9001 tcgactatga agaacttcca acgcaagatg taagagatgt tttaaataat gtttatcgaa
     9061 cattagatga acgaggatat aatgccgtaa accaaattgt aggttattta ttatcaggtg
     9121 accctgcgta tattccacgc caaaatgaag caagaaatca aattcgtcat attgatagag
     9181 atgttattat ggaagagctt gtttcttact atttaaaaga gcaaaataaa taatttatgt
     9241 tacaacataa aattttagga ctcgatgtcg gtagtagaac ggtaggaatt gcaattagtg
     9301 atataatggg ttggacggca caaggattag acacactccg aatcaatgaa gaaaataaag
     9361 aattaggtat tgaccaatta gtagacatta ttaaaaaaca taatgtgggt acagtcgtaa
     9421 taggactacc taaaaacatg aataattcaa taggatttcg aggcgaagct tcgttaacat
     9481 ataaagaaaa attattagaa gcttatcctt ctattgagat tgtgatgtgg gatgaaagat
     9541 taagcacaat ggctgctgaa cgatcattat tagaagcaga tgtttcaaga caaaaaagaa
     9601 aacaagtgat tgataaaatg gcagcggtat ttattttaca aggctattta gattcactac
     9661 attaaaagga gattttacta tgactgaaca taatcatgat tcacaactag aaattaataa
     9721 cgaagaagaa ttattaactt tattcgatga agagggaaat gaagttttat accgaaaagt
     9781 tttagaattt tatcatcctg aattcaaaaa agagtatgtt atcttagctg aagaaggtgc
     9841 tcaatcagat gaagacgata tgattgagct tgtaccaatg atcaatgaac cagatgagtc
     9901 aggtgacggt ggtaagttag taccaatcga aactgatgaa gaatgggaca tgattgaaga
     9961 agttgtaaat actgaaatgg aagaataatt aaaaagattt tactcttgct aaccttaaag
    10021 atggggaggc aagagtattt tttaagtcaa gcaatgtata aataattaac gcttataatt
    10081 ttattgaaaa tatggtaaaa
//
LOCUS       DAXWFK010000095        10037 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.095, whole genome shotgun sequence.
ACCESSION   DAXWFK010000095 DAXWFK010000000
VERSION     DAXWFK010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10037
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.095"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(88..1686)
                     /locus_tag="ACGEXS_001769"
     CDS             complement(88..1686)
                     /locus_tag="ACGEXS_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFF8012769.1"
                     /translation="MLVNTFNPFDNLLLSSLIAAIPIVLFLLCLTVFKMKGIYAAITT
                     LVVTLLIAIPFFKLPVGIASGAVVEGFFQGIIPIGYIVMMAVLLYKITVESGQFLTIQ
                     DSITNISQDQRIQVLLIGFAFNAFLEGAAGFGVPIAICALLLTQLGFNPLKAAMLCLV
                     ANAASGAFGAIGIPVGVVETLKLPGDVSVLGVSQSATLTLAIINFIIPFLLIFIIDGF
                     RGVKETLPAILVVSITYTLTQGLLTVFSGPELADIIPPLLTMLALAVFSKKFQPKHIY
                     RVNKDEEIEPAKAHSAKAVLHAWSPFIVLTVIVMIWSAPFFKNLFLPNGALSSLVFKF
                     NLPGTISEVTHKPLVLTLNIIGQTGTAILLTIIITILMSKKVNFKDAGRLFGVTFKEL
                     WLPVLTICFILAISKITTYGGLSAAMGQGIAKAGNVFPVLSPILGWIGVFMTGSVVNN
                     NSLFAPIQASVAQQIGTSGSLLVSANTVGGVAAKLISPQSIAIATAAVKQVGKESELL
                     KMTLKYSVCLLIFICIWTFILSLL"
     gene            2003..3676
                     /locus_tag="ACGEXS_001770"
     CDS             2003..3676
                     /locus_tag="ACGEXS_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HFF8012770.1"
                     /translation="MIKQINVSNMQKFESQLMKAQSEGYTHVVPYANEIMIYQSMLDA
                     VQLYPKSIVVDYTVDGQYKNDCHYFGQSSINIADWAQNNNYYPNLIYAIQQTLDLIHY
                     YSVETIFDLALLTLLKGDLSIDGHVVFDFKAPIATSASIWETIKTIEDFDMMSQFYLN
                     KMAYIDHHPIPFRNLFIEDSEQLNSPDNWLYSTKFMLPKWLYKIAKQRADNKQLQNFG
                     LYTKQPNVLKDHIVFIGDHHQYIGNSKYLFTYFVKHNPMTACYFVTDDRRGPHFISPK
                     SEKADELINSARVVIVENDIPETLQPNGTLIQLHQGTPIMQLFLDSKEPIKNIETPFY
                     RAKRYNRWLQFDYVIHSADDISHFYQTAFPSHQANVLAYGNPKHQYLLQKSNESTTQQ
                     QFKKSFKINDQKPVLLYAPIGLVSAQQLPLSDALFKAYHVVVQGVDEAILPEEALVAP
                     KYLSAQDLILMSDVVITDYSNIIFDAMAIDKTVALYTPNHSQYIESQGVNEDIWRHLS
                     KIWYTDRQLLINNLISQAIPVIKYPQIQHKEQPLESISQLILSKMTSNQ"
     gene            complement(3829..4458)
                     /locus_tag="ACGEXS_001771"
     CDS             complement(3829..4458)
                     /locus_tag="ACGEXS_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012771.1"
                     /translation="MKRLVTGLLALSLFLAACGQDSDQQKDGNKEKDDKAKTEQQDKK
                     TNDSSKDKKDNKDDSKDVNKDNKDNSANDNQQQSNSNATNNDQNQTNNNQSSNNQANN
                     NQKSSYVAPYYGQNAAPVARQIYPFNGNKNQALQQLPNFQTALNAANNEANKFGSNNK
                     VYNDYSIEEHNGNYKYVFSFKDPNANGKYSIVTVDYTGQAMVTDPNYQQ"
     gene            complement(4772..5287)
                     /locus_tag="ACGEXS_001772"
     CDS             complement(4772..5287)
                     /locus_tag="ACGEXS_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFF8012772.1"
                     /translation="MAGIIKEISEQNASELVELATRTFYDTFGSYYDDKDFDQFFKDN
                     YTVEKFTQEINHVDSFHYFYQEDGANVGYIKMNINSAQTEEMGEAYLEVQRIYFLKDF
                     QGGGRGSQLIELAEKIAQEHNKHKIWLGVWEHNPRAQAFYKRHGFKVVGEHHFQTGDV
                     TDTDLIMEKEL"
     gene            5478..6482
                     /locus_tag="ACGEXS_001773"
     CDS             5478..6482
                     /locus_tag="ACGEXS_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFF8012773.1"
                     /translation="MIETFKAFVIDKDESGKVTPTFKQLSPTDLPKGDVLIKVHYSGI
                     NYKDALATQDHNAVVKSYPMIPGIDLAGTIVESEAPGFEKGEQVIVTSYDLGVSHYGG
                     FSEYARVKSEWIIKLPDTLTLEESMIYGTAGYTAGLAIERLEKVGMNIEDGPVLVRGA
                     SGGVGTLAVLMLNELGYKVIASTGKQDVSDQLLELGAKEVIDRLPVEDDHKKPLASST
                     WQACVDPVGGEGINYVTKRLNHSGSIAVIGMTAGNTYTNSVFPHILRGVNILGIDSVF
                     TAMKLRQRVWRRLAKDLMPENLHEIKQVITFDELPEQLNKVIKHENKGRIVIDFGVDK
                     "
     gene            complement(6528..6929)
                     /locus_tag="ACGEXS_001774"
     CDS             complement(6528..6929)
                     /locus_tag="ACGEXS_001774"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8012774.1"
                     /translation="MVKVTYDIPTCEDYCALRINAGMSPKTREAAEKGLPNALFTVTL
                     YDKDRLIGMGRVIGDGGTVFQIVDIAVLKSYQGQGYGSLIMEHIMQYIKGVAVESTYV
                     SLIADYPADKLYTKFGFIPTEPDSGGMYIKY"
     gene            complement(6965..7999)
                     /locus_tag="ACGEXS_001775"
     CDS             complement(6965..7999)
                     /locus_tag="ACGEXS_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFF8012775.1"
                     /translation="MKDVTIIGGGPSGLYASFYAGLRDMSVRLIDVQSELGGKMRIYP
                     EKIIWDIGGIAPKPCHEILKDTIKQGLYFKPEVHLNERVVDIRKKAERHFEVETEAGE
                     IYTSKAVIIAIGAGIINPKQLDVKGVERYQLTNLHYVVQSYRRFKDKDVLISGGGNTA
                     LDWAHDIAKIAKSVTVVYRKEDVSGHEAMKTLVTDLNVKLCPKTRIKYLVGNDDETHI
                     SEVVLEHVESGDRHTVKFDDVIISHGFDRCNTLLSETSSKLDMHDDCRVKGFGNTTTS
                     IPGIYACGDIVYHDAKSHLIASAFSDGANAANLAKTYIQPDANAEGYVSSHHEVFKEA
                     NKTIVNKHLY"
     gene            complement(8010..8126)
                     /locus_tag="ACGEXS_001776"
     CDS             complement(8010..8126)
                     /locus_tag="ACGEXS_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012776.1"
                     /translation="MANYAIYFNNININSIMALNDNNYQFEHVNERNEYRES"
     gene            complement(8231..8662)
                     /locus_tag="ACGEXS_001777"
     CDS             complement(8231..8662)
                     /locus_tag="ACGEXS_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2871 domain-containing protein"
                     /protein_id="HFF8012777.1"
                     /translation="MRRLLYSFLFYMIIGLFSGFFYRELTKAYDFTGTTQLSLVHTHT
                     LILGMFMFLILLPLEKLFKLSSYYLFNWFFYVYNIGVIVTIGMMVTKGFFQVTGKSYS
                     PEAFAGFAGIGHTGMLAGLLLLFFLLRQAILKEPRDESFKK"
     gene            complement(8869..>10037)
                     /locus_tag="ACGEXS_001778"
     CDS             complement(8869..>10037)
                     /locus_tag="ACGEXS_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HFF8012778.1"
                     /translation="LPIGILNEDKGTTIQDKNVNIGKKLEDKLLDSDSNKIKWVKVDS
                     EKDLEKDLKDQKIFGVAIIDKDFSKDAMSKTQKVVMDSKKEEMQQKVASGEIPPQVVQ
                     QMKQKMGNQQVEVKQAKFKTIVSEGSSLQGSQIASAVLTGMGDNINAQITKQSLETLT
                     SQNVKVNAADINGLTNPVKVDNEKLNKVKDHQAGGNAPFLMFMPIWIGSIVTSILLFF
                     AFRTSNNIVVQHRIIASIGQMIFAVVAAFAGSFVYIYFMQGVQRFDFDHPNRIAIFVA
                     FAILGFVGLILGVMVWLGMKSVPIFFILMFFSMQLVTLPKQMLPESYQKYVYDWNPFT
                     HYATSVRELLYLNHHIELNSTMWMFIGFMIFGAVSSLVSAIVRKHSTKRTEVPS"
BASE COUNT         3335 a         1794 c         1442 g         3466 t
ORIGIN      
        1 cttctttcat taatgatcca taaaaaaaag atgagatgtt agaaaacatc tcaccttctg
       61 tatatttata ttttgaaata cgttttttta taataatgac aagatgaaag tccaaataca
      121 gatgaatatt agtaaacata cactgtattt caatgtcatt tttaataatt ctgattcctt
      181 accaacttgt tttactgctg cagttgcaat tgcaattgat tgtggtgaaa tcaattttgc
      241 cgctacacca ccaactgtat tagcagatac aagaagtgaa ccacttgttc caatttgttg
      301 tgcaacagaa gcttgaattg gtgcaaataa tgagttgtta tttacaactg atcctgtcat
      361 aaacacacct atccaaccta aaattggtga tagaactggg aagacattac ctgctttagc
      421 aataccttga cccattgctg cacttaaacc accataagtt gtgattttag aaattgctaa
      481 gatgaaacaa attgtaagaa ctggtaacca caactcttta aatgtaacgc cgaataatct
      541 acctgcatct ttaaagttaa cctttttaga cattaaaatt gtaataataa tagttaataa
      601 aatagctgta cctgtttgtc caataatatt taaagtcaat actaatggtt tatgcgtaac
      661 ttcgctgatt gttccaggta agttgaattt aaatactaat gatgataaag caccatttgg
      721 taagaataag tttttaaaga atggcgcact ccaaatcatt acaatgactg ttaatacaat
      781 gaatgggctc catgcatgta atactgcttt tgcagaatgt gcttttgcag gttcaatttc
      841 ttcatcttta ttaacacgat aaatgtgttt tggttggaat tttttagaaa atactgctaa
      901 tgctaacatt gttaataacg gtggaataat atctgctaat tcaggtccac tgaatacagt
      961 taataatcct tgagtaagtg tataagtgat tgaaactact aaaattgctg gtaatgtttc
     1021 tttaacacct ctaaaaccat caataataaa gataagtaag aatggaataa tgaaatttat
     1081 gattgccaat gttaaagttg ctgattgaga aacacctaat actgaaacat ctccaggtaa
     1141 tttcaacgtt tctacaacac ctacagggat accaatcgca ccaaaagcac cagacgctgc
     1201 atttgcgact aaacataaca tcgcagcttt taatggatta aatcctaatt gtgttaataa
     1261 aagtgcacaa attgcaattg gtacaccaaa tcctgctgca ccttctaaaa atgcgttgaa
     1321 tgcaaatcca ataagtaaaa cttgaatacg ttggtcttgt gaaatatttg taatactatc
     1381 ttgaattgtt aaaaattgtc cagattcaac agtaattttg tataacaata ctgccatcat
     1441 aacgatatag ccaatcggaa tgataccttg gaagaaacct tcgactactg caccagaagc
     1501 gataccaact ggcaatttga aaaatggtat tgcaattagt aatgttacaa caagtgttgt
     1561 aatagctgca taaatacctt tcattttaaa aacagttaaa catagtaaaa acagtacaat
     1621 cggtattgct gcaattaaag acgataataa taaattatca aatgggttaa acgtatttac
     1681 taacataggc tgactcttct ttccataaag tagtagtgta attgcttcat taaaatcatg
     1741 ctctggctat agcattttgt tatcgataac attttacaga acagtgagat taatgtgatt
     1801 tttttcacaa ttaaaatata ccattttttc agactattaa caatatcact ttcctattta
     1861 attgttttat ttttcatttt aaatctgttc atcaaaatga attgtactta atgtaatgaa
     1921 gtaacttata ctattcatga attttacata agaccgtgct aaaataagga agatactacg
     1981 atgaggaatg gaagttatag caatgattaa acagataaat gtttctaata tgcaaaagtt
     2041 tgaatctcaa ttaatgaaag ctcagtctga aggttataca catgtcgttc catatgccaa
     2101 tgagataatg atttatcaat cgatgttaga tgcggttcaa ttatatccta agtctatcgt
     2161 ggttgattat actgtggatg gacaatacaa aaatgattgt cactatttcg gtcaatcatc
     2221 gatcaacatt gccgattggg ctcaaaacaa taattattat cctaacctga tatatgcgat
     2281 tcaacagact cttgatttaa tacattatta ctctgtagaa acgattttcg atttagcttt
     2341 attgacactt ctgaaaggtg atttatcaat cgatggtcat gtcgtttttg attttaaagc
     2401 ccctatagca acaagtgctt caatatggga aactattaaa actattgaag attttgatat
     2461 gatgtctcag ttttatttga ataaaatggc ttatattgat catcatccta taccttttcg
     2521 caaccttttt atcgaggatt cagaacaact taattcgcca gataattggt tgtattctac
     2581 caagtttatg ctacctaaat ggttatataa aatagcaaag caacgtgctg ataacaaaca
     2641 attacaaaac ttcggcctgt atacgaagca accaaacgtt ttaaaagatc acatcgtttt
     2701 tatcggtgat catcatcaat atataggtaa ttcaaaatat ttatttactt acttcgttaa
     2761 acataatcca atgactgcct gttattttgt tactgacgat cgtcgcggac cacatttcat
     2821 atcacctaag tctgaaaaag ctgacgaatt gattaacagc gcacgcgtag ttatagttga
     2881 aaatgatata cctgaaacgt tacaaccaaa cggtacttta attcaattac atcaaggcac
     2941 accaataatg caattatttt tagatagtaa agagcctatt aaaaatatag aaacaccttt
     3001 ttatcgtgct aaaaggtata atcgctggtt gcaattcgat tatgttatac attctgctga
     3061 tgatattagc cacttttatc aaacggcttt tcctagtcac caagcaaatg tattggctta
     3121 tggtaatcct aaacaccaat atttactaca aaagagtaac gaaagtacta ctcaacaaca
     3181 gtttaagaaa tcgtttaaaa taaatgatca aaaaccagtt ttattatacg cacctattgg
     3241 attagtgagt gcgcaacaat taccactatc agatgcttta ttcaaagcct atcatgttgt
     3301 ggttcagggt gttgatgaag cgatcttacc agaagaggcg cttgttgcac caaagtatct
     3361 tagtgctcaa gacttaattc taatgtctga tgtcgttatc actgattact caaatataat
     3421 atttgatgcg atggctatag acaaaacagt agcattatac actccaaatc acagtcaata
     3481 cattgagtct caaggtgtta acgaagatat ttggcgtcat ctatcaaaaa tttggtatac
     3541 agaccgacaa ttattaatta ataatttaat ttcccaggca ataccagtga ttaaatatcc
     3601 acaaatacaa cataaagaac aaccattaga atctatttca caacttatat tgtctaagat
     3661 gacatctaat caatagtgtt taaatttctc agtggctgtg aatgaggttt aaaagtacta
     3721 taaaacgtaa actttgatac tttaaaatac gcaaaaaacg gtaaacccta attcatatta
     3781 tagagtttac cgttttattt tttaacttgc atcatagtta tattaacatt attgttggta
     3841 gtttggatca gtaaccattg cttgtccagt ataatcaacc gttacaattg aatattttcc
     3901 atttgcattt gggtctttaa aactaaacac atacttatag ttgccattat gttcttcaat
     3961 agaataatca ttatacactt tattattact accaaattta tttgcttcat tattagccgc
     4021 atttaaagct gtttggaaat ttggcaattg ctgtaaagct tgatttttat ttccattaaa
     4081 cggataaatt tgacgtgcaa ccggcgcggc attttgtcca taatatggtg caacgtaact
     4141 tgatttttga ttattattcg cttggttatt acttgattgg ttattatttg tttggttttg
     4201 gtcattgttt gttgcatttg aattagattg ttgctggtta tcgtttgcac tattatcttt
     4261 attatctttg tttacgtctt tactatcatc tttattatct ttcttatctt tagatgaatc
     4321 atttgttttt ttatcttgtt gttcagtttt cgctttatca tctttttctt tattaccgtc
     4381 tttttgttgg tcactatctt gaccacatgc agctaaaaat aatgataatg ctagtaaccc
     4441 tgtaactaat cttttcatac atatctcctc ctataattcg atattcattg aataatcttg
     4501 aaatacatat ctaccatgtg tatcttttca tggcttgtta ccaaagacgt caacattaat
     4561 atgatctgaa ttatcacaat atccataact acaatctaag gttcttttta ttatacccta
     4621 atttttgttc attattattt aatttttgtg aattttatgt tttctataaa tttaattatt
     4681 ttactttaac aattcattac gcatttagca tttcaaggta tacacaatat ttattactat
     4741 gatttcattt tatctgctgc aaaaacaatc attataactc tttttccata attaaatctg
     4801 tatccgttac atcacctgtt tgaaaatgat gttcaccaac cactttaaat ccatgacgtt
     4861 tataaaatgc ttgagcacga ggattatgct cccaaactcc tagccaaatt ttatgtttat
     4921 tatgttcttg agcaattttt tcggccaatt ctatcaattg tgaacctctt ccgccacctt
     4981 gaaagtcttt caaaaaatat atgcgctgca cttctaaata ggcctccccc atttcttcag
     5041 tttgagcact attaatattc atctttatat aaccaacatt cgcaccatct tcttgataaa
     5101 aataatgaaa tgaatctaca tggttaatct cttgtgtaaa tttttctaca gtataattgt
     5161 ctttaaaaaa ttgatcaaaa tctttgtcat catagtaaga accaaacgtg tcataaaatg
     5221 ttctagttgc taattcaact aattcactag cattttgttc tgaaatttct ttgattatcc
     5281 cagccatata aatcctccaa taaacagtga tcgaatcaaa atattactta tgttattttt
     5341 cagccaaaac tatttaaaaa tacattaaca caaatcaatt acaaattgta ttgattgtgt
     5401 gtaacatcaa taaatgatac atttattcca gtaaaatggc cgtattttca aaagagaaaa
     5461 agagaggatg tatcgttgtg atagaaacat ttaaagcgtt tgtaattgat aaagatgaga
     5521 gtggtaaagt gacaccaact ttcaaacaat tatcgcctac tgatttacct aaaggagatg
     5581 tgctgattaa agtacattac tctggtataa attataaaga tgctttagcg actcaagatc
     5641 ataatgcagt cgtaaaatcg tatcctatga ttccaggaat agatttagct ggaacaattg
     5701 ttgaatccga agcaccaggc tttgaaaaag gagaacaagt aattgtaacg agttatgacc
     5761 taggtgtcag ccattatggc ggttttagtg aatatgcgcg tgtaaaatca gaatggatta
     5821 tcaagcttcc tgatacttta acattagaag aatcaatgat atatggcaca gctggttata
     5881 ctgccggttt agcaattgaa agacttgaaa aagttggaat gaatattgaa gatggtcctg
     5941 tactcgttcg cggtgcttca ggtggtgtcg gtactttagc agtactcatg cttaatgaac
     6001 ttggttataa agttatcgca agtacaggta aacaagatgt tagcgatcaa ttacttgaac
     6061 ttggtgccaa agaagttatc gatcgacttc ctgttgaaga tgatcataaa aagccactcg
     6121 catcatcaac ttggcaagct tgtgtagacc ctgttggtgg cgaaggtatt aattatgtta
     6181 caaagcgttt aaatcatagt gggtcaattg cagttattgg tatgactgcc ggtaatactt
     6241 atactaattc tgtattccct cacattttaa gaggtgtaaa cattttagga attgactcgg
     6301 tatttactgc tatgaaatta agacagcgcg tttggcgtcg tctcgcaaaa gatttaatgc
     6361 ctgaaaattt acatgagatc aagcaagtta ttacatttga tgaacttcca gaacaactta
     6421 acaaagtaat taaacatgaa aataaagggc gcattgttat cgatttcggt gtagataaat
     6481 agtattcatg aaaaagacat cccgttatgc gagatgtctt ttttaattta gtatttgata
     6541 tacataccgc ctgaatctgg ttcggtaggt ataaatccaa attttgtata taatttatcc
     6601 gctgggtagt ctgcaatcag actaacgtat gtactctcaa cagccacacc tttaatatat
     6661 tgcataatat gctccataat tagactgccg taaccttgac cttggtaact tttcaaaact
     6721 gcaatatcaa caatttgaaa aacagttccg ccatcgccaa tcactctacc cataccaatt
     6781 aaccgatctt tatcatacaa ggttactgta aataaggcat taggtaatcc tttttcagct
     6841 gcttcgcgcg tctttggact catacctgcg ttaatcctta atgcgcaata atcctcgcaa
     6901 gtcggaatat catatgtcac tttaaccatt atttacccca cttttcatca cacaatatat
     6961 caacctagta taaatgttta tttacaatag tcttattcgc ttctttaaac acttcatgat
     7021 gacttgaaac ataaccctct gcattcgcat ctggttggat atatgtttta gcaaggttcg
     7081 ctgcatttgc accatcacta aatgcacttg caattagatg tgattttgca tcatgataaa
     7141 caatatctcc acacgcatag ataccaggta tactagttgt cgtattacca aatcctttaa
     7201 cacgacaatc atcatgcata tctagctttg aagatgtttc actcaataat gtattacaac
     7261 gatcaaaccc atgactaata atgacatcgt caaatttaac tgtatgccta tcgccacttt
     7321 caacatgttc caaaacaact tcacttatat gcgtttcatc atcattgccg accaagtatt
     7381 taatacgtgt ttttgggcat agtttcacat ttaaatctgt caccaacgtt ttcatcgctt
     7441 catgaccact tacatcttct tttcgataaa caactgtcac gcttttagca atcttggcaa
     7501 tatcatgcgc ccaatctaat gctgtatttc ctccacctga tattaataca tctttatctt
     7561 tgaaacgtct gtaactttgt acaacataat gtaaattagt taattgatat ctctctacac
     7621 ctttaacatc taattgtttt ggattaataa tacccgcacc aattgcaatg ataactgctt
     7681 tcgatgtata tatttctccc gcttctgttt caacttcgaa atgacgttct gcctttttcc
     7741 taatatctac cacacgttca ttcaaatgaa cttccggttt aaaatataat ccttgcttaa
     7801 ttgtatcttt taaaatttca tgacaaggtt ttggcgcaat gccgccaata tcccaaataa
     7861 ttttttcagg gtaaattctc atcttacccc ctaattcaga ttgaacatct atcaatctta
     7921 cagacatatc tcgcaatcca gcataaaagc ttgcatacaa accagacgga ccgccaccaa
     7981 tgattgtaac atctttcatt atgtgcctcc tatgactctc tatattcatt tctttcatta
     8041 acgtgctcaa attgataatt attatcattt aaagccatta tactattaat atttatattg
     8101 ttaaaataaa tcgcatagtt agccatgaat tatcaataac tatcccatca caatataaaa
     8161 acacaccttt caaagtaaga aacaacaatg ttcctttgaa agatgtgtat attttttagt
     8221 tctagttata ttatttttta aaagactcat cacgtggttc tttaagaatt gcttgtctta
     8281 aaaggaaaaa tagcaacaat aaacctgcaa gcatacctgt gtgcccaata cctgcaaagc
     8341 ctgcaaatgc ttctggagag tatgatttac cagtgacttg gaagaatcct tttgtcacca
     8401 tcatacctat agtaacgata acacctatgt tatatacata gaagaaccag ttaaataagt
     8461 agtaacttga taatttaaat aacttctcca atggtaataa tattaaaaac ataaacatac
     8521 ctaaaataag tgtatgtgtg tgtacaagtg ataattgtgt tgtaccagta aaatcatatg
     8581 cttttgttag ctctctatag aaaaagccac taaataaacc tatgatcata taaaaaagaa
     8641 atgaatataa taacctacgc attttgaatc ctccctcacc ttttcattat atccgattat
     8701 acctattata acagtgacaa aaaagcgaag atatgacaca actgaatttg gtttagcaca
     8761 tagtatgaca gctaaaataa ctttacatca agcgctatac aaataaaata gctataccat
     8821 gacgctcgct acaaacgtct tgtatagcta ttatttctat ttattaaatt atgacggcac
     8881 ttcagtacgt tttgtgctat gttttctaac aatagctgaa actaaacttg atacagcgcc
     8941 aaaaatcata aaccctataa acatccacat tgtactattt aattcaatat gatgattcaa
     9001 gtataatagt tctcttacac ttgttgcata gtgtgtgaat ggattccaat catatacata
     9061 tttttgataa ctttcaggca acatttgttt aggtaacgtt acaagttgca tactaaagaa
     9121 cattaaaatg aagaaaattg gaactgactt catacctagc cataccataa cacctaaaat
     9181 aaggcccacg aaaccaagaa tcgcaaatgc tacaaaaatt gcgatacgat ttggatggtc
     9241 aaaatcaaat ctttgaacgc cttgcatgaa ataaatataa acaaagctac ctgcaaatgc
     9301 tgcaacaact gcaaatatca tctgtccaat tgaagcaatg atacgatgtt gcacgacgat
     9361 attgttacta gttctaaatg caaagaacaa taagatagac gttacgattg aacctatcca
     9421 aattggcata aacattagga atggtgcatt accacctgct tggtgatctt taactttatt
     9481 aagtttttca ttatccactt ttactggatt cgtcaaacca ttgatgtccg cggcattgac
     9541 tttaacattt tgactcgtta atgtttccaa actttgcttc gtaatttgag cattaatatt
     9601 atcacccata ccagttaaca cagctgatgc aatttgtgaa ccttgtaagc ttgatccttc
     9661 acttacaatc gttttaaatt tagcctgctt aacctctact tgttgattcc ccattttttg
     9721 tttcatttgt tgaaccactt gtggcggaat ttcacctgaa gcaacttttt gttgcatttc
     9781 ttctttttta ctatccataa ctactttttg tgttttactc atagcatctt ttgaaaagtc
     9841 tttatcaata atagctactc caaagatttt ttgatctttc aaatcttttt caaggtcttt
     9901 ttcactatca accttaaccc atttaatttt attagaatca ctatctaata atttatcctc
     9961 taatttttta ccaatgttaa catttttatc ttgaatcgtt gtacctttat cctcgtttaa
    10021 tataccaatt ggtaaat
//
LOCUS       DAXWFK010000096         9656 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.096, whole genome shotgun sequence.
ACCESSION   DAXWFK010000096 DAXWFK010000000
VERSION     DAXWFK010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9656
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.096"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            34..3486
                     /locus_tag="ACGEXS_001779"
     CDS             34..3486
                     /locus_tag="ACGEXS_001779"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFF8012779.1"
                     /translation="MKQIKKLLVANRGEIAIRIFRAAAELDISTVAIYSNEDKSSLHR
                     YKADESYLVGSDLGPAESYLNIERIIDVAKQANVDAIHPGYGFLSENEQFARRCAEEG
                     IKFIGPHLEHLDMFGDKVKARTTAIKADLPVIPGTDGPIKSYELAKEFAEEAGFPLMI
                     KATSGGGGKGMRIVREESELEDAFHRAKSEAEKSFGNSEVYIERYIDNPKHIEVQVIG
                     DEHGNIVHLFERDCSVQRRHQKVVEVAPSVGLSSTLRQRICDAAIQLMENIKYVNAGT
                     VEFLVSGDEFFFIEVNPRVQVEHTITEMVTGIDIVKTQILVAAGADLFGEEINMPQQK
                     DITTLGYAIQCRITTEDPLNDFMPDTGTIIAYRSSGGFGVRLDAGDGFQGAEISPYYD
                     SLLVKLSTHAISFKQAEEKMVRSLREMRIRGVKTNIPFLINVMKNKKFTSGDYTTKFI
                     EETPELFDIQPSLDRGTKTLEYIGNVTINGFPNVEKRPKPDYELASIPTVSSSKIASF
                     SGTKQLLDEVGPKGVAEWVKKQDDVLLTDTTFRDAHQSLLATRVRTKDMINIASKTAD
                     VFKDGFSLEMWGGATFDVAYNFLKENPWERLERLRKAIPNVLFQMLLRASNAVGYKNY
                     PDNVIHKFVQESAKAGIDVFRIFDSLNWVDQMKVANEAVQEAGKISEGTICYTGDILN
                     PERSNIYTLEYYVKLAKELEREGFHILAIKDMAGLLKPKAAYELIGELKSAVDLPIHL
                     HTHDTSGNGLLTYKQAIDAGVDIIDTAVASMSGLTSQPSANSLYYALNGFPRHLRTDI
                     EGMESLSHYWSTVRTYYSDFESDIKSPNTEIYQHEMPGGQYSNLSQQAKSLGLGERFD
                     EVKDMYRRVNFLFGDIVKVTPSSKVVGDMALYMVQNDLDEQSVITDGYKLDFPESVVS
                     FFKGEIGQPVNGFNKDLQAVILKGQEALTARPGEYLEPVDFEKVRELLEEEQQGPVTE
                     QDIISYVLYPKVYEQYIQTRNQYGNLSLLDTPTFFFGMRNGETVEIEIDKGKRLIIKL
                     ETISEPDENGNRTIYYAMNGQARRIYIKDENVHTNANVKPKADKSNPSHIGAQMPGSV
                     TEVKVSVGETVKANQPLLITEAMKMETTIQAPFDGVIKQVTVNNGDTIATGDLLIEIE
                     KATD"
     gene            complement(3626..4537)
                     /locus_tag="ACGEXS_001780"
     CDS             complement(3626..4537)
                     /locus_tag="ACGEXS_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000467197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme A synthase"
                     /protein_id="HFF8012780.1"
                     /translation="MFGKKNLKWLGVVATLMMTFVQLGGALVTKTGSADGCGSSWPLC
                     HGALIPEFFPIDTIIELSHRAVSALSLLMVLWLVITAWKHIGYIKEIKPLSIISVGFL
                     LLQALIGAAAVIWQQNDYVLALHFGISLISFSSVFLITLIIFSIDQKYEADELYIKKP
                     LRRLTWLMAIIIYCGVYTGALVRHADASLAYGGWPLPFHDLVPHSEQDWVQLTHRIMA
                     FIVFTIIMITYIHAVKNYPNNRTVHYGYTAAFILVILQVITGALSIMTNVNLIIALFH
                     ALFITYLFGMTTYFIMLMLRSVRSDKQ"
     gene            4977..5888
                     /gene="cyoE"
                     /locus_tag="ACGEXS_001781"
     CDS             4977..5888
                     /gene="cyoE"
                     /locus_tag="ACGEXS_001781"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFF8012781.1"
                     /translation="MSKEHTLSQNISRVNFKELQQIIKMGLVQGNLIPAFAGAWLAVV
                     MTNHSFLSSIPQILLMLFGSTLIMGGACALNNYYDQDIDRIMPSKQNRPTVNNRITDQ
                     NLLLLSFGMMLVGEICLFLLNIPSGVLGLMGIVGYVSYYSIWSKRHTTWNTVIGSFPG
                     AVPPLIGWVAIEGQISLTAIALFLVVFCWQPIHFYALAIKRKDEYALANIPMLPSVKG
                     FKRTRVSMFIWLIILLPVPLLLINLGVVFVVLATLLNLGWIALGLTTFKKNSDQTKWA
                     TQMFIYSLNYLVIFFVLAVIVSLLTLI"
     gene            5913..6374
                     /locus_tag="ACGEXS_001782"
     CDS             5913..6374
                     /locus_tag="ACGEXS_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HFF8012782.1"
                     /translation="MGVPILPTISTTCIVISAILIAIGWRLIWKREINKHKNVMLAAA
                     VFALTFFLIYASRTIFIGNTAFGGPASIKKYYTIFLFFHINLATIGGILGLVQIITAF
                     KDKYNVHRKFGPFASVIWFCTAITGVAVYLLLYVLYPGGETTSLIKATFGH"
     gene            6701..7738
                     /locus_tag="ACGEXS_001783"
     CDS             6701..7738
                     /locus_tag="ACGEXS_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFF8012783.1"
                     /translation="MKKLIIRVVGVLFLVGFLIYLFYSPRLKFDVLENPNKGNKVNRS
                     EQVNKSNNHAENPKPKEGVGTWVGKDIKVLTSKFGQADRVYPFRDGYKNYVFKDKNSY
                     YIVSTKREEIVSVYATGEKVNVSPLKIGQHSAEIFNHTSINPEPSFKVDGKKYEFELS
                     DEDLKTQTLIKYGDIYAQVYSDQQSKKVLSVRFLTKEMLADIEPYQLNSNSTSEEHNK
                     RPVEQNPNQLISLYEVTNEMRKLKGLKPLKINSDLAHIASNNLYEATSNGSDSVEFTE
                     DALRGQLDKNHVTYKTTAQNVGYAFNDVPTLIHSWMNSDIHRSRLLNSKYDEMGGDVM
                     RDYYSLIFLEK"
     gene            7754..8188
                     /locus_tag="ACGEXS_001784"
     CDS             7754..8188
                     /locus_tag="ACGEXS_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFF8012784.1"
                     /translation="MINEASLAILDDIDELADMIVASDIYASFEQAKQALENNDEAHL
                     LYQSFLKSKEKYDEVMRFGKYHPDYKKVMLETRQRKRAYEMLDVVMHYKAREMALQHL
                     IDEVVTKIAYAVSEHVKIETGNPFFQTSHSGCATGGSCNCSL"
     gene            complement(8251..9177)
                     /locus_tag="ACGEXS_001785"
     CDS             complement(8251..9177)
                     /locus_tag="ACGEXS_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFF8012785.1"
                     /translation="MKKVNRNILNAVLVGAGFLSSLLMINKNKVITKKQTIPAFFKGN
                     APYIFAHRGGMALRPEQTQLAFDYAKQLGVDGFETDVRLTKDQQLIVFHDATVDRTTN
                     GSGKVSAHTLAELKKLDAAYHFKDINGLTPYRGHAHTAILTFDELLKQYPDMYINVDL
                     KDAPESYEGSIAPQIMFDTIAENQAFDRVLVTSFYKEQIVRFNKIAQGSVAIGASQQE
                     VTEAFLKYHLLGGRYYQPLAQTFQMPTHFKGIDLTSSRFIKWLNDMNIIPGYYGVNSI
                     NLMNDLYQKGAHTIVTDRPDLAQQFKQTIPNK"
     gene            9416..>9656
                     /locus_tag="ACGEXS_001786"
     CDS             9416..>9656
                     /locus_tag="ACGEXS_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFF8012786.1"
                     /translation="MNLIPRTSIVVYLKHMKHERQIRKYGHIVHSNRDRKFVIMYVNE
                     QDVDQIVHKLMQLKYVRHIDGSPYKYLKKTYEKEKH"
BASE COUNT         3382 a         1424 c         1765 g         3085 t
ORIGIN      
        1 gaaaattcta atagtttgag gagtgtatcg ctattgaaac aaataaaaaa gttacttgtt
       61 gctaaccgtg gagaaattgc aattcgtata ttcagagcgg cggcagaatt agacatcagc
      121 acagttgcaa tttattcgaa tgaagacaaa agttcattac atagatataa agcagatgaa
      181 tcctatttag ttggaagtga tttaggtcct gctgaaagtt atttaaatat tgagcgtatc
      241 attgatgtag caaaacaagc gaatgtggat gcgattcatc ctggctatgg atttttaagt
      301 gaaaatgaac aatttgcgcg tcgttgtgca gaagaaggaa ttaaatttat tggtcctcat
      361 ttagaacatt tagatatgtt tggagataaa gttaaagctc gtacaacggc tatcaaggca
      421 gatttaccag ttattcctgg tacagacggt ccaattaaat catatgaatt agcaaaagaa
      481 tttgcagaag aagctggttt cccgctaatg attaaagcca caagtggtgg cggcggtaaa
      541 ggtatgagaa tcgttcgtga agaaagtgaa ttagaagatg ctttccatag agcaaaatca
      601 gaagctgaaa aatcatttgg taatagtgaa gtttacatag aaagatacat tgataatcca
      661 aagcatattg aagtacaagt cataggtgac gaacatggaa atatcgtaca cttatttgaa
      721 cgtgattgtt cagtacaacg tcgtcatcaa aaagttgtag aagttgcacc atcagttgga
      781 ttatcatcaa cattacgtca acgtatttgt gatgctgcaa ttcaattgat ggaaaatatt
      841 aaatatgtca atgcaggtac tgttgaattt ctagtatctg gtgacgaatt cttctttata
      901 gaagttaacc ctcgtgtaca agtagagcat acaattacag agatggtaac aggaattgat
      961 attgttaaga cacaaatttt agttgcagca ggtgccgatt tatttggtga agagattaat
     1021 atgccgcaac aaaaagatat tacaacatta ggctatgcca tccaatgtcg tattacaaca
     1081 gaagatccgt taaatgattt catgccggat actggaacaa tcattgctta tcgttcaagc
     1141 gggggctttg gtgtacgtct tgatgctgga gatggtttcc aaggtgctga gatatcacct
     1201 tattatgatt cattactcgt aaaattatct acacacgcga tatcatttaa acaagcagaa
     1261 gaaaaaatgg tacgctcatt acgagaaatg cgtattcgtg gtgttaaaac taatattcca
     1321 ttcttaatta atgtaatgaa gaataaaaag ttcacaagtg gtgattacac aactaaattt
     1381 attgaagaaa caccagaact tttcgacatt cagccgtctc tagatagagg tactaaaaca
     1441 ttagaatata taggtaatgt aacaattaat ggtttcccaa atgttgagaa acgtccgaaa
     1501 ccagactatg aattagcatc aattccaact gtatcttcaa gtaaaatcgc ttcatttagt
     1561 ggtacgaaac aattgcttga tgaagtaggt ccaaaaggtg tagctgaatg ggttaaaaag
     1621 caggatgatg tcttactaac agatacaacc tttagagatg cacaccaatc attattagct
     1681 acacgagtta gaactaagga tatgattaat atcgcatcca aaacagcgga cgtatttaaa
     1741 gatggtttct cactagaaat gtggggcggt gctacatttg atgtggcata taatttcttg
     1801 aaggaaaacc catgggaacg acttgaacgt ctacgtaaag ctattccaaa tgtattattc
     1861 caaatgttgt tacgtgcttc aaacgcagtt ggttataaaa actatcctga taatgttatt
     1921 cataaattcg tacaagaaag tgctaaagca ggcatagatg tctttagaat tttcgattca
     1981 ttaaactggg tagatcaaat gaaagttgcc aatgaagcag tacaagaagc gggcaaaatc
     2041 tcagaaggta ctatttgtta tacaggtgac attttaaatc ctgagcgatc aaacatttat
     2101 actttagagt attatgtcaa actagctaaa gagttagaac gtgaaggttt ccatatttta
     2161 gcgattaaag atatggcagg cttattaaaa cctaaagccg cttacgaatt gattggtgag
     2221 ttaaaatcag ctgtagattt accaattcat cttcacactc atgatacaag tggtaatggt
     2281 ttattaacat acaaacaagc aatagatgct ggtgtcgata tcattgatac tgctgttgct
     2341 tcaatgagtg gtttaacaag tcagccaagc gccaattcgt tatattatgc attaaatggc
     2401 ttcccacgcc accttagaac tgatattgaa ggtatggagt cacttagtca ttattggtca
     2461 actgtacgta cttattattc agactttgaa agtgatatca aatcaccgaa tactgaaatt
     2521 tatcaacatg aaatgcctgg tggacagtat tcgaatttaa gtcaacaagc taaaagttta
     2581 ggtttaggcg aaagatttga tgaagtcaaa gatatgtatc gcagagtgaa tttcttattt
     2641 ggtgatatcg taaaagtaac accatcgtct aaagtagttg gtgatatggc actttatatg
     2701 gtacaaaatg atcttgatga acaatccgtg attacagatg gctataaatt agatttccca
     2761 gaatcagtag tgtcgttctt caaaggtgaa ataggacaac ctgtaaatgg ttttaataaa
     2821 gatttacaag cggttatttt aaaaggccaa gaagcactaa cagctcgtcc aggtgaatat
     2881 ctagagccag ttgattttga aaaagtccgt gagttgcttg aagaagagca acaaggtcct
     2941 gttacggagc aagatattat tagttatgta ctatatccaa aagtatatga acaatatatt
     3001 caaactagaa atcaatacgg aaacttatcg ttacttgata cgcctacatt cttctttgga
     3061 atgcgtaatg gtgaaacagt agaaatcgaa atcgataaag gtaaacgatt aattattaaa
     3121 ctagaaacga ttagtgaacc agatgaaaat ggtaatagaa cgatttacta tgcgatgaat
     3181 ggtcaagcga gacgtattta cattaaagat gaaaatgtgc atacaaatgc gaacgttaag
     3241 ccaaaagcag ataagagtaa tccaagtcat atcggtgctc aaatgccagg ttcagtaact
     3301 gaagtcaagg ttagtgtagg tgaaactgtg aaagctaatc agccgttgct aattactgaa
     3361 gctatgaaaa tggaaacaac aattcaagca ccatttgacg gtgtgattaa acaagtaact
     3421 gtaaataatg gtgacacaat agcgacaggc gatttattaa tcgaaattga aaaagcaact
     3481 gactaaaatg attaaaataa aacgagatta cacaacaatt gacgtgtaat ctcgttttta
     3541 catggcttag gtttaatttt aaatattgat ataaaaaacc gtaattttaa tgggcgtgcc
     3601 attaaaatta cggtttgctt ttttgttatt gcttgtcact tcttactgat cgtaacatta
     3661 gcatgataaa gtatgttgtc ataccaaata aataagtgat aaataatgca tggaaaagtg
     3721 cgattatcag gttaacattt gtcataatag ataatgcacc tgtgataact tgtaatataa
     3781 caagtataaa tgcagctgta taaccataat gaacagtacg gttatttgga taatttttaa
     3841 cagcgtgaat ataagtaatc ataataatcg taaacactat aaaagccatg atacgatgcg
     3901 tgagttgaac ccaatcttgt tctgaatgtg gtacaagatc gtggaatggc aatggccaac
     3961 caccatatgc taaacttgca tccgcatgtc tcactagtgc accagtataa acaccacaat
     4021 aaatgatgat tgccattaac catgttaaac gtcttaatgg ctttttgata tataattcgt
     4081 cagcttcata tttttgatct atagagaaaa taatcaatgt tattaaaaat acagatgaga
     4141 aactgattaa tgatatacca aagtgcaatg ctaaaacgta atcgttttgt tgccaaataa
     4201 cagcagcagc tccgattaat gcttgcaata ataagaatcc aacactaatg attgataaag
     4261 gtttaatttc tttaatatag cctatatgtt tccatgcagt gataactaac cataagacca
     4321 ttaataaaga caaagctgaa acggctctat gacttaactc aataatcgta tcaataggaa
     4381 agaattctgg aatcaacgca ccatgacata gtggccaaga agaaccacaa ccatcagctg
     4441 atccggtttt ggtaactaag gctccaccaa gttgtacaaa tgtcatcatt aacgttgcta
     4501 cgacacctaa ccattttaaa ttctttttgc caaacaattt atacacccca tcataaaaaa
     4561 acgcatcact gagaattaga attaatagcc ggatttgaga tgatactata aatagacctg
     4621 attgtaggat atgaaaagat gggatccaac gcaggaaata tgatttaatt ttacaagtat
     4681 tgtatgtgga cgacataaaa agaacttaat cgtaatgatt tttttattgg gataattatg
     4741 taatccacgt ttactcaaat ttgaagctta aatattaatc tcttttagat gcatgattct
     4801 acttattaaa attttctaac atatacatta tagcaataaa ttaaatgtta tttgtgtcac
     4861 aaaattgaca ttttctcttg tcgttaattt gtcacaattt aattttatta aacctagtct
     4921 ttttatgcaa tctaagatat cataatctta tatgaacaaa tttaaggagg gggattatga
     4981 gcaaagagca tactttgtca caaaatatta gcagagttaa cttcaaagaa ttgcaacaga
     5041 taattaaaat gggacttgtt caaggtaact taattccggc ttttgcgggc gcatggctag
     5101 cagttgtaat gacaaatcat tccttcttat catcaatacc tcaaatttta ttaatgctat
     5161 ttggatctac tttaattatg ggtggcgcat gtgcgttaaa taattattac gaccaagata
     5221 ttgatcgtat tatgcctagt aaacaaaata gaccaactgt aaataataga attacagatc
     5281 aaaatttatt actattaagt tttggtatga tgttagttgg agaaatttgt ttgtttttat
     5341 tgaatatacc atcaggcgta cttggtctta tggggattgt aggttatgtg tcttattact
     5401 caatatggtc taaaagacat acaacatgga acacagtgat tgggagtttt cctggagcag
     5461 taccaccact aattggatgg gttgcaattg aaggacaaat tagtttaaca gcgattgcgc
     5521 tgtttttagt tgtattttgt tggcaaccaa ttcattttta tgccttagct attaaacgta
     5581 aagatgaata tgcacttgca aatattccaa tgttaccatc agttaagggc tttaaacgta
     5641 cacgtgtcag tatgtttatc tggttgatta ttttattgcc agtaccttta ttactaataa
     5701 atttaggtgt cgtattcgta gtgttagcta cattattaaa tttaggatgg attgcattag
     5761 gtttaacaac atttaagaaa aattcagatc aaacaaaatg ggcaacacaa atgtttatat
     5821 attcactaaa ttatttagtg atctttttcg tgttagctgt gattgtttca ttacttactt
     5881 tgatctagat taattaagtt aggatgaaaa atatgggcgt tccaatttta ccaacgatta
     5941 gtacgacatg tattgtcatt agtgcaattt taattgccat tggttggaga cttatttgga
     6001 aaagggaaat aaataaacac aaaaatgtta tgttagctgc agctgttttt gctttaactt
     6061 ttttcttaat ctatgcaagt agaacgattt ttatcggtaa tacagcattt ggcggaccag
     6121 catcaattaa gaaatattat acgattttct tatttttcca cattaattta gcaacaattg
     6181 gtggtattct aggcctagtt caaattatta ctgcatttaa agataaatat aatgtacacc
     6241 gcaaattcgg gccattcgct tcagttatat ggttctgtac tgcaattaca ggtgtagcag
     6301 tttacttatt attatatgta ttatatccag gtggagaaac gacatcactg attaaagcaa
     6361 catttggtca ttaatatgct tatacaaatg atactaatct gataaggcaa tttgttttat
     6421 caggtttttt gatgaagaat agtcggacag ttgatggtat tcatttgata ccttaactgt
     6481 ccattttttt atacatattt taatcttaat aatgaaattc aacctgaaaa cataaagata
     6541 ggttatgata tagagtgtag ggaaaatctt tatgacgtta aaattatgcc tgaagatgat
     6601 tttgttttat cgaaacgaca cagttatgtc agtgttgaaa atttgctaat taatgtgttg
     6661 ttttttacaa tagtattaat actttttagg tggttgattg atgaaaaagt taattataag
     6721 agtcgtcggt gtattatttt tagttggatt tttaatatat cttttttatt caccgagatt
     6781 aaaatttgat gtattagaga acccgaataa aggtaataaa gtaaatagat ctgaacaggt
     6841 gaataaatca aataaccatg ctgaaaatcc aaagcctaaa gaaggtgttg gtacatgggt
     6901 aggtaaagat attaaagtgc ttacttctaa atttggacaa gcagatcgtg tctacccttt
     6961 tagagatggt tacaaaaatt atgtgtttaa agacaaaaac agttattaca ttgtttcaac
     7021 taaacgtgaa gaaatcgttt cagtgtatgc tacaggtgag aaagtcaatg ttagtccgtt
     7081 aaaaatagga caacattctg cagaaatttt taatcataca agtattaatc cagaaccgtc
     7141 ctttaaagtt gatggtaaaa aatatgaatt tgaactttca gatgaagatt taaaaacaca
     7201 aacactgatt aaatatggcg acatatatgc acaagtgtat tctgatcaac aatcgaaaaa
     7261 ggtgctaagt gtacgatttt taacaaaaga aatgttagca gatattgaac cttatcaatt
     7321 aaattctaat tctacgtcag aagagcataa taagcgtcca gttgagcaaa atccaaatca
     7381 attaatttct ctttatgaag taacgaatga aatgagaaaa ttaaaaggat taaaaccatt
     7441 gaaaatcaat agcgatttag cacatattgc atctaataac ttatatgaag cgacctctaa
     7501 tggttctgat agtgttgaat ttacagagga cgcattaaga gggcaattag ataaaaatca
     7561 cgttacttat aaaacaactg ctcaaaatgt tggttatgcg tttaatgatg taccaacatt
     7621 aatccatagt tggatgaatt cagatataca tcgatctcgt ctattaaatt caaaatacga
     7681 tgagatgggt ggagatgtaa tgagagatta ttattcacta attttcttag aaaaataaca
     7741 atgaggggta tttatgatta atgaagcttc actagcgata ttagatgata ttgatgaact
     7801 agctgatatg atagttgcat cagatattta tgcatcattt gagcaagcta aacaggcgct
     7861 cgaaaacaat gacgaagcac atttacttta tcaatcattt ttaaaatcaa aagagaaata
     7921 tgatgaagta atgcgattcg ggaaatatca tcctgattat aagaaagtta tgttagagac
     7981 aagacaacgc aaaagagcat atgagatgct tgacgttgtg atgcattaca aagctaggga
     8041 aatggcactt caacatttaa tagatgaagt tgttacgaaa attgcgtacg ctgtttcaga
     8101 acatgtcaaa atagaaacag gtaatccatt ctttcaaaca tcacatagtg gttgtgcgac
     8161 gggcggatcc tgtaattgtt cattataaaa aacatcgagt cagaaaaagg tggttattga
     8221 aaccactaac tagcatctga ctcgatgttt ttatttattc gggattgttt gtttgaattg
     8281 ttgtgctaaa tctggtcgat ctgtcacaat cgtgtgtgca cctttttggt ataaatcatt
     8341 catcagattt atactattta cgccataata gcctggaatg atattcatat catttaacca
     8401 tttgataaaa cgagatgaag tcaaatcaat gcctttaaaa tgagtaggca tttggaacgt
     8461 ttgtgctaat ggttggtagt acctaccacc taataaatga tattttaaaa atgcttctgt
     8521 aacttcctgt tggctagcac caattgcgac ggatccttgt gcaattttat taaaacgaac
     8581 gatttgttct ttataaaaac ttgtcacaag aacgcggtca aatgcttgat tttctgcaat
     8641 tgtatcaaac ataatttgtg gtgcgattga gccttcatag gattcaggag catcttttaa
     8701 gtctacgttt atatacatat caggatattg cttcagcaac tcatcgaagg ttagtatagc
     8761 tgtgtgtgca tgaccacgat atggtgttag tccattgata tctttgaagt gataagctgc
     8821 gtctaatttc tttaattctg ctaatgtatg ggcactaact tttccagagc cgttcgtcgt
     8881 tctatcaaca gttgcgtcat gaaaaacgat aagctgttga tcttttgtga gtctcacatc
     8941 tgtttcaaag ccatcaacgc ctaattgttt agcatagtca aatgcaagtt gcgtttgctc
     9001 tggtcttaaa gccataccac cgcgatgcgc aaatatatat ggtgcattgc ctttgaaaaa
     9061 agcagggatg gtttgctttt tagtaatcac tttattttta ttgatcatta atagactact
     9121 taaaaatcca gcaccgacta gtaccgcatt taaaatgttt ctgtttactt ttttcataaa
     9181 aaattcctcc tcgtttactt ttatagctta acaaattcat agttctgact acagcgaaat
     9241 gtgtggttaa tcatatagtg atgctcttga gaattgaatt aatataatga tgtttaatat
     9301 aatctaaata catttatcga gcaattacat aagacataat taaggtagct taatatttta
     9361 aaattaataa agacatgata tgattaatgc tgaaaataaa tggagtgagg tttaaatgaa
     9421 tttaatccca agaactagta ttgtagttta tttaaaacat atgaaacatg aacgacaaat
     9481 ccgaaaatat ggacatatcg ttcattcaaa tagagatcgt aaatttgtaa ttatgtatgt
     9541 gaatgagcaa gatgttgatc aaattgtaca taaactaatg caacttaaat acgttagaca
     9601 cattgatggc tcaccatata aatacttaaa gaaaacttac gaaaaagaga aacacg
//
LOCUS       DAXWFK010000097         9522 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.097, whole genome shotgun sequence.
ACCESSION   DAXWFK010000097 DAXWFK010000000
VERSION     DAXWFK010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9522
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.097"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(125..964)
                     /gene="noc"
                     /locus_tag="ACGEXS_001787"
     CDS             complement(125..964)
                     /gene="noc"
                     /locus_tag="ACGEXS_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0032466 - negative regulation of
                     cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HFF8012787.1"
                     /translation="MKKPFSKLFGLKNKDDIIGHIEEDRNSNVESIQIERIVPNRYQP
                     RQVFEPNKIKELAESIHEHGLLQPIVVRPIEEDMFEIIAGERRFRAIQSLNLPQADVI
                     IRDMDDEETAVVALIENIQRENLSVVEEAEAYKKLLEIGDTTQSELAKSLGKSQSFIA
                     NKLRLLKLAPKVLLRLREGKITERHARAVLSLSDSEQEALIEQVIAQKLNVKQTEDRV
                     RQKTGPEKVKAQNLRFAQDVTQARDEVGKSIQAIQQTGLHVEHKDKDHEDYYEIKIRI
                     YKR"
     gene            complement(1007..1726)
                     /gene="rsmG"
                     /locus_tag="ACGEXS_001788"
     CDS             complement(1007..1726)
                     /gene="rsmG"
                     /locus_tag="ACGEXS_001788"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFF8012788.1"
                     /translation="MTVEWLAEQLKEHNIQLTETQKQQFQTYYRLLVEWNEKMNLTSI
                     TDEHDVYLKHFYDSIAPSFYFDFNQPISICDVGAGAGFPSIPLKIMFPQLKVTIVDSL
                     NKRIQFLNHLASELQLQDVSFIHDRAETFGKGVYRESYDVVTARAVARLSVLSELCLP
                     LVKKGGQFVALKSSKGEEELEEAKFAISVLGGNVTETHTFELPEDAGERQMFIIDKKR
                     QTPKKYPRKPGTPNKTPLLEK"
     gene            complement(1726..3603)
                     /gene="mnmG"
                     /locus_tag="ACGEXS_001789"
     CDS             complement(1726..3603)
                     /gene="mnmG"
                     /locus_tag="ACGEXS_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFF8012789.1"
                     /translation="MVQEYDVIVIGAGHAGVEAGLASARRGAKTLMLTINLDNIAFMP
                     CNPSVGGPAKGIVVREIDALGGQMAKTIDKTHIQMRMLNTGKGPAVRALRAQADKVLY
                     QQEMKRVIEDEENLHIMQGMVDELIIEDNEVKGVRTNIGTEYLSKAVIITTGTFLRGE
                     IILGNMKYSSGPNHQLPSITLSDNLRELGFDIVRFKTGTPPRVNSKTIDYSKTEIQPG
                     DDVGRAFSFETTEYILDQLPCWLTYTNAETHKVIDDNLHLSAMYSGMIKGTGPRYCPS
                     IEDKFVRFNDKPRHQLFLEPEGRNTNEVYVQGLSTSLPEHVQRQMLETIPGLEKADMM
                     RAGYAIEYDAIVPTQLWPTLETKMIKNLYTAGQINGTSGYEEAAGQGLMAGINAAGKV
                     LNTGEKILSRSDAYIGVLIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLRLTDMGY
                     ELGMISEERYARFNEKRQQIDAEIKRLSDIRIKPNEHTQAIIEQHGGSRLKDGILAID
                     LLRRPEMTYDIILELLEEEHQLNADVEEQVEIQTKYEGYINKSLQQVEKVKRMEEKKI
                     PEDLDYSKIDSLATEAREKLSEVKPLNIAQASRISGVNPADISILLIYLEQGKLQRVS
                     D"
     gene            complement(3670..5049)
                     /gene="mnmE"
                     /locus_tag="ACGEXS_001790"
     CDS             complement(3670..5049)
                     /gene="mnmE"
                     /locus_tag="ACGEXS_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFF8012790.1"
                     /translation="MDLDTITSISTPMGEGAIGIVRLSGPQAVEIADKLYKGKHLLND
                     VPSHTINYGHIIDPESKEVVEEVMVSVLRAPKTFTREDIIEINCHGGILTINRVLELT
                     MTYGARMAEPGEFTKRAFLNGRIDLSQAEAVMDFIRSKTDRASKVAMNQIEGRLSDLI
                     KKQRQSILEILAQVEVNIDYPEYDDVEDATTEFLLEQSKEIKQEINRLLDTGAQGKIM
                     REGLSTVIVGKPNVGKSSMLNNLIQDNKAIVTEVAGTTRDVLEEYVNVRGVPLRLVDT
                     AGIRETEDIVEKIGVERSRKALSQADLILFVLNNNEALTQEDYTLYEVVKNEDVIVIV
                     NKMDLEQNIDINEVKDMIGDTPLIQTSMLKQEGIDELEIQIRDLFFGGEVQNQDMTYV
                     SNSRHISLLKQARQTIQDAIDAAESGVPMDMVQIDLTRTWEILGEIIGETASDELIDQ
                     LFSQFCLGK"
     gene            complement(5188..5541)
                     /gene="rnpA"
                     /locus_tag="ACGEXS_001791"
     CDS             complement(5188..5541)
                     /gene="rnpA"
                     /locus_tag="ACGEXS_001791"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFF8012791.1"
                     /translation="MLLEKAYRIKKNADFQRIYKKGHSVANRQFVVYTCNNKEIDHFR
                     LGISVSKKLGNAVLRNKIKRAIRENFKVHKSHILAKDIIVIARQPAKDMTTLQIQNSL
                     EHVLKIAKVFNKKIK"
     gene            complement(5662..5799)
                     /gene="rpmH"
                     /locus_tag="ACGEXS_001792"
     CDS             complement(5662..5799)
                     /gene="rpmH"
                     /locus_tag="ACGEXS_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFF8012792.1"
                     /translation="MVKRTYQPNKRKHSKVHGFRKRMSTKNGRKVLARRRRKGRKVLS
                     A"
     gene            6530..7891
                     /gene="dnaA"
                     /locus_tag="ACGEXS_001793"
     CDS             6530..7891
                     /gene="dnaA"
                     /locus_tag="ACGEXS_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFF8012793.1"
                     /translation="MSEKEIWEKVLEIAQEKLSAVSYSTFLKDTELYTIKDGEAIVLS
                     SIPFNANWLNQQYAEIIQAILFDVVGYEVKPHFITTEELANYSNNETATPKETTKPST
                     ETTEDNHVLGREQFNAHNTFDTFVIGPGNRFPHAASLAVAEAPAKAYNPLFIYGGVGL
                     GKTHLMHAIGHHVLDNNPDAKVIYTSSEKFTNEFIKSIRDNEGEAFRERYRNIDVLLI
                     DDIQFIQNKVQTQEEFFYTFNELHQNNKQIVISSDRPPKEIAQLEDRLRSRFEWGLIV
                     DITPPDYETRMAILQKKIEEEKLDIPPEALNYIANQIQSNIRELEGALTRLLAYSQLL
                     GKPITTELTAEALKDIIQAPKSKKITIQDIQKIVGQYYNVRIEDFSAKKRTKSIAYPR
                     QIAMYLSRELTDFSLPKIGEEFGGRDHTTVIHAHEKISKDLKEDPIFKQEVENLEKEI
                     RNV"
     gene            8169..9302
                     /gene="dnaN"
                     /locus_tag="ACGEXS_001794"
     CDS             8169..9302
                     /gene="dnaN"
                     /locus_tag="ACGEXS_001794"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFF8012794.1"
                     /translation="MMEFTIKRDYFITQLNDTLKAISPRTTLPILTGIKIDAKEHEVI
                     LTGSDSEISIEITIPKTVDGEDIVNISETGSVVLPGRFFVDIIKKLPGKDVKLSTNEQ
                     FQTLITSGHSEFNLSGLDPDQYPLLPQVSRDDAIQLSVKVLKNVIAQTNFAVSTSETR
                     PVLTGVNWLIQENELICTATDSHRLAVRKLQLEDVSENKNVIIPGKALAELNKIMSDN
                     EEDIDIFFASNQVLFKVGNVNFISRLLEGHYPDTTRLFPENYEIKLSIDNGEFYHAID
                     RASLLAREGGNNVIKLSTGDDVVELSSTSPEIGTVKEEVDANDVEGGSLKISFNSKYM
                     MDALKAIDNDEVEVEFFGTMKPFILKPKGDDSVTQLILPIRTY"
BASE COUNT         3054 a         1750 c         1396 g         3322 t
ORIGIN      
        1 ccagcctgat caacatataa atataaattt ttatgtttca cgtaaaacaa aaaaaaagac
       61 aaagctgtta tgatcttagc tttgtctttt atgtgttagt catcatgtat acgacatcct
      121 actactaacg tttatatatt cgaattttta tttcataata atcttcatga tctttgtctt
      181 tatgctcaac atgtaatcct gtttgttgaa tcgcttggat acttttgcct acctcatctc
      241 gtgcttgagt gacatcttgt gcaaagcgaa ggttttgtgc tttgactttt tcgggccccg
      301 ttttttggcg tactctatct tcagtctgtt tcacatttag cttttgtgca atgacttgct
      361 caatcaacgc ttcttgttcg ctatcagaca atgataatac cgctctcgca tgacgttcag
      421 taattttacc ttctcttaag cgaagtagta ctttcggcgc caacttcaat aaacgcaact
      481 tatttgcaat aaagctttga cttttaccta aactttttgc caattcactt tgcgttgtat
      541 caccaatttc caataatttc ttataggctt ccgcttcttc aacaacagac aaattttctc
      601 tttgaatatt ctcaattaat gcaacaacag ccgtctcttc atcatccata tcacgaataa
      661 taacgtctgc ttgaggtaaa tttagtgatt gtattgctct aaatcggcgc tctccagcaa
      721 taatttcaaa catatcttct tcaatcggtc ttacaacaat aggttgtagt aaaccatgtt
      781 catgtattga ttcagcaagt tctttaattt tatttggttc aaacacctgt cttggttgat
      841 aacggttggg aacgatacgt tcaatttgaa tggattcaac attactattg cgatcttctt
      901 caatatgtcc aatgatgtca tctttgtttt tcaaaccaaa taattttgaa aaaggttttt
      961 tcattatcca ttcgctcctt ttatgttagt tgtaaaggat tatgcattat ttttcaagta
     1021 aaggagtctt attaggcgtc cctggttttc ttggatactt tttcggcgtc tgtctttttt
     1081 tatcaataat gaacatctgg cgctctccag catcttctgg caattcaaag gtatgtgttt
     1141 ctgtaacatt accacctaac acactaattg caaattttgc ttcttctaat tcttcttcac
     1201 cttttgaaga ttttaatgca acaaactgtc cacctttttt aactagcggt aaacacaatt
     1261 cacttaacac ggataatcta gctactgctc ttgcagtaac aacatcataa gactccctgt
     1321 agacaccctt accaaatgtt tctgctctat cgtgtataaa gctgacatcc tgtaattgta
     1381 attctgacgc taaatggttt aaaaattgaa tacgcttatt taatgaatca acaatcgtca
     1441 cttttaactg cggaaacatt atttttaacg gaatacttgg aaaaccagct ccagcgccta
     1501 catcacatat acttataggc tgattaaaat caaaataaaa actaggtgca atggaatcat
     1561 aaaaatgttt caaatataca tcgtgttcat ctgtaatact tgtcaaattc atcttttcat
     1621 tccattcaac aagtaaacga taatatgttt gaaactgttg tttttgagtc tcagttaatt
     1681 gaatattatg ttcttttaat tgttctgcta accattctac agtcattagt cactcaccct
     1741 ttggagttta ccttgttcta agtaaatcaa taatatagat atgtctgctg gatttacccc
     1801 tgatattcta gaagcttgtg caatatttaa aggttttact tctgataatt tttctcgcgc
     1861 ttcagtcgcc aaactatcaa tcttactata atctaagtct tctggaattt tcttctcttc
     1921 catacgctta actttctcaa cttgttgtag tgatttattg atataacctt catattttgt
     1981 ttgtatttct acttgttctt caacatctgc attcaattga tgttcttctt ctaaaagttc
     2041 taaaattata tcgtaagtca tttcaggtct gcgtaataaa tcgatagcta aaataccatc
     2101 ttttaagcga gaaccaccat gttgttcaat aatcgcttgc gtatgttcgt ttggtttaat
     2161 acgaatatct gataaacgct taatttccgc atcaatttgc tgacgttttt cattaaaacg
     2221 tgcatatctt tcttcagaaa tcataccaag ttcatatccc atatccgtca atctcaaatc
     2281 agcattatca tgacgtagta acaaacgata ttctgcacgt gatgttagta aacgataagg
     2341 ttcattagta cctttagtta caagatcatc gattaagaca ccaatatatg catctgaacg
     2401 acttaatatc ttttcgcctg tgtttaacac tttacctgca gcgttaatac ctgccatcaa
     2461 tccttgtcct gctgcttctt cataaccaga tgtaccatta atttgacctg cagtatataa
     2521 gtttttaatc attttcgttt caagtgtagg ccataactgc gttggcacaa tcgcatcata
     2581 ttcaattgcg tagccggcac gcatcatatc tgctttttca agacctggta tcgtctctaa
     2641 catttgacgt tgcacatgtt caggaagact tgtagacaat ccttgcacat atacttcatt
     2701 tgtattacga ccttcaggct ctaagaaaag ttgatgtcgc ggcttatcat taaatcgaac
     2761 aaatttatct tcaattgaag ggcaataacg tggcccggtt cctttaatca tccctgaata
     2821 cattgcagat agatgtaaat tatcatcgat aactttgtgt gtttcagcat tagtatacgt
     2881 tagccaacat ggcaattgat ctaatatata ttctgttgtt tcaaagctga atgcacgacc
     2941 tacatcgtca cctggttgta tttcagtctt cgaatagtca attgtttttg aatttacacg
     3001 cggtggtgta cctgttttaa aacgaacaat atcaaaacca agttctctta aattgtctga
     3061 taatgtgatt gatggtaatt ggtgatttgg tccacttgaa tacttcatat tacctaaaat
     3121 gatttcacca cgtaaaaatg ttcccgttgt aataattact gctttagata aatactctgt
     3181 accaatattt gtacgtacac ctttaacttc attatcttct ataataagtt cgtctaccat
     3241 accttgcatt atatgcaaat tttcttcatc ttcaatcacg cgtttcattt cttgttgata
     3301 aagtacttta tctgcttgcg ctcttagtgc tcttacagca ggtcctttac ctgtatttaa
     3361 cattctcatt tgaatgtgtg ttttatcgat tgtttttgcc atttgtccac ctaaagcatc
     3421 aatttcacga acaacgatac ctttagctgg tccacctaca gatgggttac atggcataaa
     3481 tgcaatatta tctaaattta ttgttagcat taatgtttta gcaccacgtc ttgcagatgc
     3541 taaacctgct tctacacctg catgtcccgc acctataacg attacatcat attcttgaac
     3601 cacaatataa acctccttat ttgatatctt actagccttc ttaagacggt attccgtcta
     3661 tttcaattac tatttaccta agcagaattg actgaataac tgatcgatga gttcatcact
     3721 tgcagtctca ccaataattt ctcctaatat ttcccaagtt ctagttaaat caatttgtac
     3781 catatccata ggcacaccag attctgctgc atcaatcgca tcttgtatcg tttgtcttgc
     3841 ttgttttaat aatgaaatat gtcttgaatt agaaacataa gtcatatctt gattttgtac
     3901 ttctccacca aagaacaaat ctcgaatttg tatttctaat tcatcaatac cttcttgttt
     3961 taacattgaa gtttgaatta atggcgtatc acctatcata tctttaactt cattaatatc
     4021 tatgttttgc tctaaatcca ttttattaac aattacgatt acatcttcat ttttaaccac
     4081 ttcatataat gtgtaatctt cttgagtcaa tgcttcgtta ttgtttaata caaataaaat
     4141 taagtctgct tggctaagag cctttctaga gcgttcaaca ccaatcttct ctactatatc
     4201 ttctgtctca cgtataccag cagtatcaac taatcttaat ggcacgccac gaacattgac
     4261 gtactcttct aagacatctc tagtagtacc tgctacctca gttacaatcg ctttattatc
     4321 ttgtattaaa ttatttaaca tcgatgattt acctacgttt ggtttaccaa caataactgt
     4381 agataaacct tcacgcataa ttttaccctg cgcaccggta tctaataaac gattaatttc
     4441 ctgtttgatt tctttagact gctctaaaag aaattcagta gtcgcatctt caacatcatc
     4501 gtattcagga taatcaatat tcacttccac ttgagcgagt atctctaata tagattgacg
     4561 ttgttttttg attaagtcac ttagacgacc ttcaatttga ttcatcgcaa ctttagaagc
     4621 tctatctgtc ttcgagcgaa taaagtccat aactgcttca gcttgagata aatcaatacg
     4681 accatttaaa aaggcacgtt ttgtaaattc acctggctca gccattctag cgccatatgt
     4741 catagtaagt tccagcactc tattaatcgt taaaatacca ccatgacaat taatttctat
     4801 aatatcttcg cgtgtaaatg tttttggcgc tcttaacaca gacaccataa cttcttcaac
     4861 cacttcttta gactctggat caataatatg accgtaatta atcgtatgtg atggaacatc
     4921 atttaaaaga tgttttcctt tatataattt gtcagcaatt tcaacggctt gcggtccaga
     4981 caatcgaaca attccaattg ccccttcacc cattggtgtt gaaatactcg taattgtatc
     5041 taaatccata ttgctactcg cctccttcaa cgatgtgaat acattttaaa gtaagttatt
     5101 ataaccctaa ggtcagtctt acgtttgtct gaggtaagac ttcgggatgt gttgagtggt
     5161 taatgttttc cttcccctac cctatcctta cttaatcttt ttattaaaaa ctttggcaat
     5221 tttaagtacg tgctcaagac tattctgtat ttgtaaagtc gtcatatctt tagctggctg
     5281 tcttgctatt acaataatat ctttggccaa tatatgcgac ttatgtactt tgaaattttc
     5341 acgtattgct cttttaatct tgtttcttaa cactgcatta cctagttttt tagaaacact
     5401 aatacctaag cgaaaatggt ctatttcttt attattacaa gtgtatacaa caaattgtct
     5461 gttggctaca gaatgacctt ttttatatat tctctgaaaa tctgcattct ttttaattcg
     5521 gtaagctttt tccaataaca tcactcgctt atttatcgtt tttatttgaa gctatattta
     5581 aacttctatt gagcttataa cataaatttc tatttattct taatttaaac gaaaaaaaag
     5641 atcactgatg agtcagtgat cttatgcaga taaaacttta cggcctttac gacgacggcg
     5701 cgctaaaact ttacggccat tttttgtgct catgcgtttt ctgaaaccat gaactttact
     5761 atgtttacgt ttatttggtt gataagtacg ttttaccatg caaaacacct ccatctttat
     5821 acagttatct aatataccag attatgcaga ctctgatcac tttaatagat aagattttgc
     5881 gtttcttaga ttaaaattct cgtattagct cattgattat ctagtcacaa ttcaagcaac
     5941 tactacaata taacaaaatc ctatttataa cgcaagttca ttttatacta ctgctcaatt
     6001 tttttacttt tatcgattaa agatagaaat acacgatgcg agcaatcaaa tttcataaca
     6061 tcaccatgag tttggtccga agcatgagtg tttacaatgt tcgaacacct tatacagttc
     6121 ttatacatac tttataaatt atttcccaaa ctgttttgat acactcacta acagatactc
     6181 tatagaagga aaagttatcc acttatgcac atttatagtt ttcagaattg tggataatta
     6241 gaaattacac acaaagttat actattttta gcaacatatt cacaggtatt tgacatatag
     6301 agaactgaaa aagtataatt gtgtggataa gtcgtccaac tcatgatttt ataaggattt
     6361 atttattgat ttttacataa aaatactgtg cataactaat aagcaagata aagttatcca
     6421 ccgattgtta ttaacttgtg gataattatt aacatggtgt gtttagaagt tatccacggc
     6481 tgttattttt gtgtataact taaaaattta agaaagatgg agtaaattta tgtcggaaaa
     6541 agaaatttgg gaaaaagtgc ttgaaattgc tcaagaaaaa ttatcagctg taagttactc
     6601 aactttccta aaagatactg agctttacac gattaaagat ggtgaagcta tcgtattatc
     6661 gagtattcct tttaatgcaa attggttaaa tcaacaatat gctgaaatta tccaagcaat
     6721 cttatttgat gttgtaggct atgaagttaa acctcacttt attactactg aagaattagc
     6781 aaattatagt aataatgaaa ctgctactcc aaaagaaaca acaaaacctt ctactgaaac
     6841 aactgaggat aatcatgtgc ttggtagaga gcaattcaat gcccataaca catttgacac
     6901 ttttgtaatc ggacccggta accgctttcc acatgcagcg agtttagctg tggccgaagc
     6961 accagccaaa gcgtacaatc cattatttat ctatggaggt gttggtttag gaaaaaccca
     7021 tttaatgcat gccattggtc atcatgtttt agataataat ccagatgcca aagtgattta
     7081 cacatcaagt gaaaaattca caaatgaatt tattaaatca attcgtgata acgaaggtga
     7141 agctttcaga gaaagatatc gtaatatcga cgtcttatta atcgatgata ttcagttcat
     7201 acaaaacaag gtacaaacac aagaagaatt tttctatact tttaatgaat tgcatcagaa
     7261 taacaagcaa atagttattt cgagtgatcg accaccaaag gaaattgcac aattagaaga
     7321 ccgattacgt tcacgctttg aatgggggct aattgttgat attacgccac cagattatga
     7381 aactcgaatg gcaattttgc agaagaaaat tgaagaagaa aaattagata ttccaccaga
     7441 agctttaaat tatatagcaa atcaaattca atctaatatt cgtgaattag aaggtgcatt
     7501 aacacgttta cttgcatatt cacaattatt aggaaaacca attacaactg aattaactgc
     7561 tgaagcttta aaagatatca ttcaagcacc aaaatctaaa aagattacca tccaagatat
     7621 tcaaaaaatt gtaggccagt actataatgt tagaattgaa gatttcagtg caaaaaaacg
     7681 tacaaagtca attgcatatc cgcgtcaaat agctatgtac ttgtctagag agcttacaga
     7741 tttctcatta cctaaaattg gtgaagaatt tggtgggcgt gatcatacga ccgtcattca
     7801 tgctcatgaa aaaatatcta aagatttaaa agaagatcct atttttaaac aagaagtaga
     7861 gaatcttgaa aaagaaataa gaaatgtata agtaggaaac tttgggaaat gtaatctgtt
     7921 atataacagc actaatgata acaatcattt tttacatttc tatatgctaa tgtggcaaga
     7981 tgagcaaaac tcattttgtg gataatgttt aaaagtcata cacaccatac acaagttatc
     8041 aacatgtgta taacttcgcc aaatctatgt ttttaagact tatccaccaa tccacagcac
     8101 ctactactat tactaagaac ttaaaaccta tataattata tataaacgac tggaaggagt
     8161 tttaattaat gatggaattc actattaaaa gagattattt tattacacaa ttaaatgaca
     8221 cattaaaagc tatttcacca agaacaacat tacctatatt aactggtatc aaaatcgatg
     8281 cgaaagaaca tgaagttata ttaactggtt cagactctga aatttcaata gaaatcacta
     8341 ttcctaaaac tgtagatggc gaagatattg tcaatatttc agaaacaggc tcagtagtac
     8401 ttcctggacg attctttgtt gatattataa aaaaattacc tggtaaagat gttaaattat
     8461 ctacaaatga acaattccag acattaatta catcaggtca ttctgaattt aatttaagtg
     8521 gcttagatcc agatcaatat cctttattac ctcaagtttc tagagatgac gcaattcaat
     8581 tgtcggtaaa agtgcttaaa aacgtgattg cacaaacaaa ttttgcagtg tccacctcag
     8641 aaacacgccc agtactaact ggtgtgaact ggcttataca agaaaatgaa ttaatatgca
     8701 cagcgactga ctcacaccgc ttggctgtaa gaaagttgca gttagaagat gtttctgaaa
     8761 acaaaaatgt catcattcca ggtaaggctt tagctgaatt aaataaaatt atgtctgaca
     8821 atgaagaaga cattgatatc ttctttgctt caaaccaagt tttatttaaa gttggaaatg
     8881 tgaactttat ttctcgatta ttagaaggac attatcctga tacaacacgt ttattccctg
     8941 aaaactatga aattaaatta agtatagaca atggggagtt ttatcatgcg attgatcgtg
     9001 cctctttatt agcgcgtgaa ggtggtaata acgttattaa attaagtaca ggtgatgacg
     9061 ttgttgaatt gtcttctaca tcaccagaaa ttggtactgt aaaagaagaa gttgatgcaa
     9121 acgatgttga aggtggtagc ctgaaaattt cattcaactc taaatatatg atggatgctt
     9181 taaaagcaat cgataatgat gaggttgaag ttgaattctt cggtacaatg aaaccattta
     9241 ttctaaaacc aaaaggtgac gactcggtaa cgcaattaat tttaccaatc agaacttact
     9301 aaaaataaat ataaataaag gatgacgtga ttaattaaaa cgtcatcctt tattttttgg
     9361 caaaaataat tctaggtgcg tatgtaaaat aaatttggca gcattttaaa cagcaaataa
     9421 aagacgccaa ttaaatttat gacaaatgta tccaaaattt aataagtgtg cttatatgcc
     9481 ctttaaattt aaaattttaa tagtcaataa caagttgaat at
//
LOCUS       DAXWFK010000098         9445 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.098, whole genome shotgun sequence.
ACCESSION   DAXWFK010000098 DAXWFK010000000
VERSION     DAXWFK010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9445
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.098"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..102
                     /locus_tag="ACGEXS_001795"
     CDS             <1..102
                     /locus_tag="ACGEXS_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus minor pilin ComGF family protein"
                     /protein_id="HFF8012795.1"
                     /translation="VTAFTANIYYKSIIKITITVKVGTNVQTKTIYV"
     gene            199..345
                     /locus_tag="ACGEXS_001796"
     CDS             199..345
                     /locus_tag="ACGEXS_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012796.1"
                     /translation="MMLTAIYFSIFTFYISQYSLKLKTLYNIETDYNYKIVLLLKEGK
                     THES"
     gene            335..859
                     /locus_tag="ACGEXS_001797"
     CDS             335..859
                     /locus_tag="ACGEXS_001797"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFF8012797.1"
                     /translation="MNHDKSPIILIGFMGTGKSTIGKYVADEQNLSFIDIDSYIEEKY
                     KLTIPEIFCKHGEQYFRNLEFTCLQECINTADIIATGGGIIESEEAFNFLKNQKNIIW
                     LDCNIDIIYSRINDDPHRPNANNKTIKQLNDLYCSRNLRYNEIAFKKFDSHLLSISEI
                     YYELLNLIKASDQY"
     regulatory      846..943
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            1018..2109
                     /gene="gcvT"
                     /locus_tag="ACGEXS_001798"
     CDS             1018..2109
                     /gene="gcvT"
                     /locus_tag="ACGEXS_001798"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFF8012798.1"
                     /translation="MSSDLKQTPLYQNYVDRGAKIVEFGGWAMPVQFSSIKEEHNAVR
                     YEIGLFDVSHMGEIEVTGKDASQFVQYLLSNDTDNLTTSKALYTALCNEEGGIIDDLV
                     IYKLADDNYLLVVNAANTEKDFNWILKHKEKFDVEVQNVSNQYGQLAIQGPKARDLIN
                     QLVDEDVTEMKMFEFKQGVKLFGANVILSQSGYTGEDGFEIYCNIDDTEKIWDGLLEY
                     NVMPCGLGARDTLRLEAGLPLHGQDLTESITPYEGGIAFASKPLIDADFIGKSVLKDQ
                     KENGAPRRTVGLELLEKGIARTGYEVMDLDGNIIGEVTSGTQSPSSGKSIALAMIKRD
                     EFEMGRELLVQVRKRQLKAKIVKKNQIDK"
     gene            2129..3475
                     /gene="gcvPA"
                     /locus_tag="ACGEXS_001799"
     CDS             2129..3475
                     /gene="gcvPA"
                     /locus_tag="ACGEXS_001799"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HFF8012799.1"
                     /translation="MSHRYIPLTEKDKQEMLQTIGAKSIGELFGDVPSDILLNRDLNI
                     AEGEAETTLLRRLNRIASKNITKETHTSFLGAGVYDHYAPSVVDAMISRSEFYTAYTP
                     YQPEISQGELQAIFEFQTLICELTDMDVANSSMYDGMTSFAEACILAFSQTKKNKIVV
                     SKGLHYQALQVLHTYAKTRKEFEVVEIDLDGTVTDLKKLEAAVDDETAAVAVQYPNFY
                     GSIEDLEKIHSFIEDKKALFIVYANPLALGLLTPPGSFGADIVVGDTQPFGIPAQFGG
                     PHCGYFATTKKLMRKVPGRLVGQTQDDEGNRGFVLTLQAREQHIRRDKATSNICSNQA
                     LNALASSIAMSALGKQGIYDIAVQNIEHANYAKQQFIKKGFEVLDGTSFNEFVVKFDK
                     PIQQVNEELVKYNIIGGFDLGVVSDDFKNHMLIAVTELRTKDEIDTFVEKAGELND"
     gene            3468..4940
                     /gene="gcvPB"
                     /locus_tag="ACGEXS_001800"
     CDS             3468..4940
                     /gene="gcvPB"
                     /locus_tag="ACGEXS_001800"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HFF8012800.1"
                     /translation="MTSKSSPLIFERSREGRYAYSLPKSDIKTNSVESLLDDKFIRKN
                     KAEFPEVAELDLVRHYTELSNKNFGVDNGFYPLGSCTMKYNPKINEKVARIPGFSESH
                     PLQDEDQVQGSLEIIYSLQEELKEITGMDEVTLQPAAGAHGEWTALMIFKAYHENNGE
                     GHRDEVIVPDSAHGTNPASASFAGFKSVTVKSNERGEVDIDDLKRVVNENTAAIMLTN
                     PNTLGIFEKNIMEIREIVHNAGGLLYYDGANLNAIMDKVRPGDMGFDAVHLNLHKTFT
                     GPHGGGGPGSGPVGVVKELASYLPKPMVIKDGDKFKYDNDIKNSIGRVKPFYGNFGIY
                     LRAYTYIRTMGATGLKEVSEAAVLNANYIKARLSKHFEIPYKQYCKHEFVLSGVRQKE
                     FGVRTLDMAKRLLDFGVHPPTIYFPLNVEEGMMIEPTETESKETLDYFIDTLISIAEE
                     AKNDPDKVLEAPHTTVIDRLDEATAARKPILKFENLKQEK"
     gene            complement(5305..5691)
                     /locus_tag="ACGEXS_001801"
     CDS             complement(5305..5691)
                     /locus_tag="ACGEXS_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFF8012801.1"
                     /translation="MSASLYIAIILVIAIIAYMIVQQILNKRAVKELDQNEFHNGIRK
                     AQVIDVREKVDYDYGHINGSRNIPMTMFRQRFQGLRKDQPVYLCDANGIASYRAARIL
                     KKNGYTDIYMLKGGYKKWTGKIKSKK"
     gene            5849..6679
                     /locus_tag="ACGEXS_001802"
     CDS             5849..6679
                     /locus_tag="ACGEXS_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HFF8012802.1"
                     /translation="MTETWNFINTGSKDPYYNMAMDEALLNFVSRGEIDPVIRFYTWN
                     PATLSIGYFQRLQKEIDIDKVKEKGFGLVRRQTGGRGVLHDKELTYSVIVPESHPNMP
                     STVTEAYRVISQGLLEGFKNLGFDTYFAVPKTPEERQKLKQPRSSVCFDAPSWYELVV
                     EGRKIAGSAQTRQKGVILQHGSILQDIDIDELFDMFIYKNERLKLKMKEAFVEKAVAI
                     NDISDEHITISQMEEAFEKGFKKGLNIELKPLELTEAQLAEVEELTEKYRSDEWMFRK
                     "
     gene            complement(6743..6961)
                     /locus_tag="ACGEXS_001803"
     CDS             complement(6743..6961)
                     /locus_tag="ACGEXS_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SA1362 family protein"
                     /protein_id="HFF8012803.1"
                     /translation="MRNIIFYLVLIIAAIGLVMNLDAFIFSIVRMLISFAVIAGIIYL
                     IYYFFILTEDQRKYRKAMRKYKRNQRRK"
     gene            complement(6975..7556)
                     /locus_tag="ACGEXS_001804"
     CDS             complement(6975..7556)
                     /locus_tag="ACGEXS_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012804.1"
                     /translation="MKKLVSIVGATLLLAGCGSQNLAPLEEKTTDLREDNHQLKLDIQ
                     ELNQQISDSKSKIKGLEKDKENSKKTASNNTKIKLMNVTSTYYDKVAKALKSYNDIEK
                     DVSKNKGDKNVQSKLNQISNDIQSAHTSYKDAIDGLSLSDDDKKTSKNIDKLNSDLNH
                     AFDDIKNGYQNKDKKQLTKGQQALSKLNLNAKS"
     gene            7661..8722
                     /locus_tag="ACGEXS_001805"
     CDS             7661..8722
                     /locus_tag="ACGEXS_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFF8012805.1"
                     /translation="MSRITQVHRILEQKHLDAIIILSDYNRRYLSGFTGTSGALIISK
                     DKQYLITDFRYIDQATKQAPNYEIINRKSTIIGEIKELLHQENFENVGFEGHHVSYDT
                     YLELNKSRISLISISNTVDKIRDVKDADEIALIQKAANIVDETYEYILTVVKAGMTEK
                     ELKAILESKMLELGADGPSFDTIVASGHRGALPHGVASDKIIEKGDMITLDFGAYYNG
                     YCSDITRTFAIGEPDPKLKEIYQIVLESQMKAINEIRPGMTGAEADAISRNYLESKGY
                     GKEFGHSLGHGIGLEIHEGPMLARTIQDKLQVNNCVTVEPGVYIEGLGGIRIEDDILI
                     TENGCQVFTKCTKDLIVLT"
     gene            8748..9305
                     /gene="efp"
                     /locus_tag="ACGEXS_001806"
     CDS             8748..9305
                     /gene="efp"
                     /locus_tag="ACGEXS_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFF8012806.1"
                     /translation="MISVNDFKTGLTISVDNAIWKVIDFQHVKPGKGSAFVRSKLRNL
                     RTGAIQEKTFRAGEKVEPAMIENRRMQYLYADGDNHVFMDNESFEQTELSSDYLKEEL
                     NYLKEGMEVQIQTYEGETIGVELPKTVELTVTETEPGIKGDTATGATKSATVETGYTL
                     NVPLFVNEGDVLIINTGDGSYISRG"
BASE COUNT         3369 a         1271 c         1721 g         3084 t
ORIGIN      
        1 gttacagcat ttactgcaaa tatctactat aaatccatta ttaaaataac gataacagtt
       61 aaagttggta caaatgtgca gactaaaact atttatgtat aacttactag tatttaaatg
      121 agtactatta aaaaggatgt gaaataattt attaattttg atttatataa agaaaaagga
      181 tatgttacgc cgttcttatt gatgctaaca gcaatttatt tttccatttt cactttttat
      241 atcagtcaat atagtttaaa attaaagaca ttatataata ttgaaactga ctataactat
      301 aagattgttt tgttattgaa agaagggaaa acgcatgaat catgataaat caccaataat
      361 tttaattggt ttcatgggta ccggtaaatc tacgattggt aaatacgttg cagatgagca
      421 aaatttatca tttattgata tagattcata tatcgaagag aagtataagt taacaatacc
      481 agaaatattt tgtaaacatg gtgaacaata tttcaggaat ttagagttca catgtttgca
      541 agaatgtatt aacactgcag atataattgc tactggtggt ggtattattg agagtgaaga
      601 ggcatttaat tttttgaaaa atcaaaaaaa cattatttgg ttagattgta atattgatat
      661 tatatatagt cgaatcaatg atgacccaca tcgacctaat gcaaataata agacaatcaa
      721 gcagttaaat gacttgtatt gctcgcggaa tttaagatat aatgaaatcg cattcaagaa
      781 atttgatagt catttgctat caatttcaga aatatattat gaattgctaa atttaataaa
      841 agcgagtgat cagtattaga gagaatagag cgttaagact ctatcgccga aggtgcaagt
      901 aatttattac gaaactctca ggcaaaagga taatactgta acgcgttcct gaattggtga
      961 tttataaaca gggtagcgat tgctatcctg tttttataat tttaaggggg tatttcaatg
     1021 tcaagtgatt taaaacaaac acctttatat caaaattatg ttgatagagg tgcaaaaatt
     1081 gtggaattcg gaggatgggc gatgcctgtt caattttcaa gtattaaaga ggagcataat
     1141 gctgttcgat acgaaattgg cctgtttgat gttagtcata tgggtgaaat tgaagtaaca
     1201 ggtaaagatg ctagtcagtt tgtgcaatat ttattatcaa atgatactga taatttaact
     1261 acttcaaaag cattatatac tgctttatgt aatgaagaag gcggtattat tgatgattta
     1321 gtaatatata aattagctga cgacaattat ttattagttg ttaatgctgc taatactgaa
     1381 aaagatttta attggatttt aaaacacaaa gagaaatttg atgttgaagt acaaaatgta
     1441 tcaaaccaat atggtcaatt agcaatacaa ggaccaaaag ctagagattt aattaatcaa
     1501 ttagttgatg aagatgtaac tgaaatgaaa atgtttgaat ttaaacaggg tgtcaaatta
     1561 tttggagcaa acgtcatttt atctcagtca ggttacacag gtgaagatgg gtttgaaatt
     1621 tattgtaata tcgatgatac tgaaaaaatt tgggatggtt tattagagta taatgttatg
     1681 ccatgtgggt taggcgctcg tgatacatta agattagagg ctggattgcc attacatgga
     1741 caagatttaa ctgaatcaat tacaccatat gaaggtggta tcgcttttgc aagtaaacca
     1801 ttaattgatg ctgattttat tggtaaatct gtattaaaag atcaaaaaga aaatggtgca
     1861 ccaagaagaa cagtgggatt agaattactt gaaaaaggaa ttgcaagaac tggttatgaa
     1921 gttatggatt tagatggaaa tattattgga gaagtaactt caggaacaca gtctccatca
     1981 tcaggaaaat caattgcact tgcaatgata aaaagagatg agtttgaaat gggtagagag
     2041 ttgcttgttc aagttcgtaa gcgtcaatta aaagcgaaaa ttgttaagaa aaatcaaatt
     2101 gataaataat taaaaagggg tgtgcattgt gagtcatcgt tatatacctt taactgaaaa
     2161 agacaagcaa gaaatgttac aaacaattgg tgcaaaatct ataggagaat tattcggtga
     2221 tgtaccaagt gacattttat taaatagaga tttaaatatt gctgaaggcg aagcggaaac
     2281 aacgttactt agaagattaa atcgcattgc aagcaagaat atcactaaag aaacgcatac
     2341 atcatttttg ggtgctggtg tttatgatca ttacgcgcca tcagttgtgg atgctatgat
     2401 ttcaagatca gaattttaca cagcatatac gccatatcaa ccggaaattt ctcaaggtga
     2461 attacaagcc atttttgagt tccaaacttt aatttgtgaa ttaacagata tggatgtagc
     2521 aaattcttct atgtatgatg ggatgactag ctttgctgaa gcatgtatct tagcatttag
     2581 tcaaacaaag aaaaataaaa ttgtagtctc aaaaggttta cattatcaag cattacaagt
     2641 acttcatact tatgctaaga cgcgtaagga attcgaagta gtggaaatcg atttagatgg
     2701 cactgttaca gatttgaaaa aattagaagc agcagtggat gatgaaacag cagctgttgc
     2761 agtacaatat ccaaactttt acggttcaat tgaagatctt gaaaagattc atagctttat
     2821 tgaagataaa aaagcattat ttatcgtata tgcaaatcca ttagcattgg gattacttac
     2881 acctccaggt tcatttggtg cagatattgt tgtaggtgat actcagccat tcggaattcc
     2941 tgctcaattc ggtgggccac attgtggata ttttgcaacg acgaaaaagt taatgcgtaa
     3001 agtgccaggt agattagtcg gtcaaacaca agatgatgaa ggtaatcgtg gctttgtatt
     3061 aacattacaa gcacgtgaac aacatattcg acgcgataaa gcgacatcta atatttgttc
     3121 taaccaagca ttaaatgctt tagcgtcatc aattgcaatg tcagcacttg gtaaacaagg
     3181 tatctatgat attgcagttc aaaatataga acatgctaac tatgctaaac aacaatttat
     3241 taaaaaaggt tttgaagtat tagatggtac atctttcaat gagtttgtag tcaaatttga
     3301 caaaccaatt caacaagtta acgaggaatt agtgaagtac aatattatcg gtggttttga
     3361 tttaggtgtt gtttcagacg actttaaaaa tcacatgtta atagctgtaa ctgaattaag
     3421 aacaaaagat gaaatcgata catttgtaga aaaggctggt gagttaaatg actagtaaat
     3481 caagtccatt aatttttgaa agatctcgtg aaggcagata tgcatattca ttaccaaaaa
     3541 gtgatattaa aacaaattct gttgagtcat tgttagatga taaatttatt cgtaaaaaca
     3601 aagcggagtt tcctgaagtt gctgaactag acttagtacg tcattatact gaactttcaa
     3661 ataaaaattt cggtgtagac aacggatttt atccattggg ttcatgtacg atgaagtata
     3721 accctaaaat taatgaaaag gttgctagaa taccaggatt tagtgaatca catccattac
     3781 aagatgaaga tcaagttcaa ggttcattag aaattattta tagtttgcaa gaagaattaa
     3841 aagaaattac tggtatggat gaggtgacat tacaaccagc tgctggcgca catggtgaat
     3901 ggactgcatt gatgatattt aaagcttacc atgagaataa tggtgaaggt catcgtgatg
     3961 aagtcattgt gccagattct gcgcatggta cgaatccagc ctcagcttca tttgcaggat
     4021 ttaaatcagt tactgtaaaa tcaaacgaac gtggcgaagt tgatattgat gacttgaaac
     4081 gtgttgtaaa tgaaaataca gcagctatta tgttaactaa tccaaacact ttaggtattt
     4141 tcgaaaaaaa tattatggaa atccgtgaaa tcgtccataa tgctggtggt ctattatatt
     4201 atgatggtgc gaatttaaac gctattatgg acaaagttcg cccaggagat atgggatttg
     4261 atgctgttca tttaaacttg cataaaacat ttactggtcc acatggtggt ggcggtcctg
     4321 gttcaggtcc agtcggtgta gtaaaagaac tagcaagtta cttaccaaag ccaatggtta
     4381 ttaaagatgg cgacaaattt aaatatgata atgacattaa aaattctatc ggacgtgtaa
     4441 aaccatttta tggtaacttt ggtatttact taagagctta tacgtatatt cgaactatgg
     4501 gagcaactgg acttaaagag gtttctgaag cagcggttct taatgcgaat tatattaaag
     4561 cacgtttatc taaacacttt gaaatacctt ataaacaata ttgtaaacac gagtttgtgt
     4621 taagtggtgt gcgtcaaaaa gaatttggtg tacgtacttt agacatggct aagcgattat
     4681 tagatttcgg tgtacatcca ccaacaatat acttcccatt aaatgttgaa gaaggtatga
     4741 tgattgaacc gactgagaca gagtctaaag aaacacttga ttattttatc gatacattaa
     4801 ttagtattgc tgaagaagct aaaaatgatc ctgataaagt gctagaagca ccacatacaa
     4861 ctgtgattga tcgattagac gaagctacag ctgctcgtaa accaatatta aagtttgaaa
     4921 atcttaaaca ggaaaaataa agtattaaac acatattccg agaattatta ttctaacttt
     4981 gtatgaagat ttaaggataa tggtttcaaa atcaattgaa aaagacaatt tctatttaaa
     5041 caagaaaact aaaccgaagt aataactctt agggtttggt attattcttt catagaaatt
     5101 gtcttttcat tttttagatt gcggtaattg aatcgtattg aaaatgagct gaactttctt
     5161 tattatgctg aaactaagtt taatgatcag tgaagctaga gaaatatagt aacagacttt
     5221 gttaaaaaat taaaatctga gtattgttat tgatgatcaa ttaacactca gattttatta
     5281 aatcgtatat taaatttaca aaaactattt tttagacttt atttttccag tccatttttt
     5341 atagccgcct tttaacatat agatatctgt atatccattc tttttcaaaa tacgagcggc
     5401 tctatagcta gcaatcccat tggcatcaca taagtatacc ggttgatctt ttcttaatcc
     5461 ttggaatcgt tgcctgaaca ttgtcatagg aatattgcga gacccattaa tgtgaccgta
     5521 gtcatagtca actttctctc taacatcgat gacttgagct tttctaatcc cattatggaa
     5581 ttcattttga tctaattctt taacagctcg cttgttaaga atttgttgaa caatcatata
     5641 agcaataatt gctataacta aaattattgc gatgtacaaa ctagcactca tcttgcatcc
     5701 tccctaatta aacgataata taattataag actgttaggt caaattatca aaatatttca
     5761 ctaaagaatt gcaattatta ttcatttagt taaattgatt aattagcgaa tcaaatatta
     5821 tacattttgc tgataggagt tcttgaatat gactgaaact tggaatttta ttaatacagg
     5881 aagtaaagac ccatattata atatggcaat ggatgaagcg ttattaaatt ttgtctctag
     5941 aggtgaaatt gatccagtta tacgttttta cacatggaat cctgcaacat tatcaatagg
     6001 gtatttccaa aggctacaaa aagaaattga tattgataag gttaaagaga aaggttttgg
     6061 tctcgtaagg cgtcaaactg gtggccgtgg ggtgctacat gataaagagt taacttacag
     6121 tgtgattgtg cctgaatcgc atccgaatat gccttctaca gttacagaag cgtacagagt
     6181 gatttcacaa ggattattag aaggatttaa aaacttagga tttgatactt attttgcggt
     6241 acctaaaacg cctgaagagc gtcaaaaatt aaaacaacct cgaagttcag tgtgttttga
     6301 tgcaccaagt tggtatgagc tagttgttga aggccgtaag attgctggga gtgctcaaac
     6361 gagacaaaag ggcgtaatcc tccaacacgg ttcgatttta caagatattg atattgatga
     6421 actgtttgat atgtttattt ataaaaatga aagattaaaa ttaaaaatga aagaggcatt
     6481 cgttgaaaaa gcagttgcca tcaatgatat ttcagatgaa cacattacta ttagtcaaat
     6541 ggaagaagct ttcgagaaag gttttaagaa aggtttaaat atagaattaa agccacttga
     6601 attaacagaa gctcaattgg cagaagtgga agaattaact gaaaaatatc gttcagatga
     6661 atggatgttt agaaagtagt ttttagaata aaaaatggaa gcacgtagac tattttacct
     6721 acaagtgctt ccgttttttt atctattttc ttctttgatt tcttttatac ttacgcattg
     6781 ctttgcgata tttgcgttgg tcttcagtta agatgaagaa ataataaatc agataaataa
     6841 taccagctat tacagcaaag ctgattaaca ttctgacgat tgaaaaaata aaggcatcta
     6901 gattcattac taatccaatc gcagcaataa taagtacaag ataaaatatt atattacgca
     6961 ttaaaagact cctatcatga ttttgcattt aagtttaatt ttgacaacgc ttgttgtcct
     7021 tttgtaagtt gttttttatc tttattttga tagccatttt taatatcatc aaatgcatga
     7081 ttcaaatcag agtttaattt atcgatattt ttagacgttt ttttatcatc atcactaagt
     7141 gataaaccat cgatagcatc tttgtatgaa gtgtgagcac tttgaatatc attagaaatt
     7201 tgatttaatt tcgattgaac attcttatcg cctttgtttt tacttacatc tttctcaata
     7261 tcgttatagg atttcaaagc tttagcaact ttgtcgtagt atgttgatgt aacattcatc
     7321 aatttaattt tcgtattatt agatgcagtt tttttactgt tttctttatc cttttcaagc
     7381 cctttaattt tagatttaga atcactaatt tgttgattaa gttcttgaat atctagtttg
     7441 agttgatgat tatcttctct taaatctgtt gttttttctt ctaatggtgc taaattttgt
     7501 gatccacatc cagctaacaa taatgttgcg ccaacaattg aaaccaattt tttcattact
     7561 atctccctaa aatcaatatc tattattttt tatccttatt atgatattat tttaatgtat
     7621 aaagttgaaa tgcacaaacg tttcactagg aggtaataag atgagcagaa taacacaagt
     7681 gcatcgtatt ttagaacaaa aacatttaga tgcgattatc attctttcgg attacaatag
     7741 aagatattta tcaggtttta ctggtaccag tggagcactt atcatatcta aagataaaca
     7801 atatttgata acagacttta gatatattga tcaagcaact aagcaggcgc caaattatga
     7861 aattattaat cgtaaatcta ctattattgg tgagattaaa gaattgctac accaagaaaa
     7921 ttttgaaaat gtaggttttg aggggcatca tgtaagttat gatacatacc ttgaattaaa
     7981 taaaagccgt atatcattaa taagcatttc taatactgta gataaaatta gagacgtcaa
     8041 agatgctgac gaaattgctt taattcaaaa agcagctaat attgttgatg aaacatatga
     8101 atatatttta actgttgtaa aagcaggcat gactgaaaaa gaattaaagg caatattaga
     8161 aagcaaaatg ctagaattag gagcagatgg accatcattc gatacgattg tagcatctgg
     8221 tcatagaggt gcattaccac atggtgttgc aagtgataaa attattgaaa aaggcgacat
     8281 gattacatta gattttggcg cgtattataa cggctattgt tcagatatta ctagaacatt
     8341 tgctattgga gaaccagatc ctaaactgaa agaaatatat caaatagtac ttgaatctca
     8401 aatgaaagca attaatgaga ttagacctgg catgactggt gcagaagctg atgccatttc
     8461 aagaaactat ttagagtcaa aagggtatgg aaaagaattt ggacattcac taggacatgg
     8521 tattggttta gaaatccatg aagggccaat gctggctcgt acgatacaag ataaacttca
     8581 agttaacaac tgtgttacag tagaacctgg tgtttatata gaaggtttgg gcggtataag
     8641 aatagaagat gatattttaa ttacagaaaa tggttgtcaa gtctttacta aatgcacaaa
     8701 agaccttata gttttaacat aagcgtgtaa aatgaggagg aaactgaatg atttcggtta
     8761 atgattttaa aacaggttta acaatttctg ttgataacgc tatttggaaa gttatagact
     8821 tccaacatgt aaagcctggt aaaggttcag cattcgttcg ttcaaaatta cgtaatttaa
     8881 gaactggtgc aattcaagag aaaacgttta gagctggtga aaaagttgaa ccagcaatga
     8941 ttgaaaatcg tcgcatgcaa tatttatatg ctgacggaga taatcatgta tttatggata
     9001 atgaaagctt tgaacaaaca gaactttcaa gtgattactt aaaagaagaa ttgaattact
     9061 taaaagaagg tatggaagta caaattcaaa catacgaagg tgaaactatc ggtgttgaat
     9121 tacctaaaac tgttgaatta acagtaactg aaacagaacc tggtattaaa ggtgatactg
     9181 caactggtgc cactaaatcg gcaactgttg aaactggtta tacattaaat gtacctttat
     9241 ttgtaaacga aggtgacgtt ttaattatca acactggtga tggaagctac atttcaagag
     9301 gataatctct aatttgttaa caaatagctt gtattcacta tactgattta acgtaagata
     9361 ttctaaataa gtctcataaa gctattgcct aaaatgatta taggttatat gctgatatga
     9421 ggctttttat ttttaaaata atttt
//
LOCUS       DAXWFK010000099         9367 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.099, whole genome shotgun sequence.
ACCESSION   DAXWFK010000099 DAXWFK010000000
VERSION     DAXWFK010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9367
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.099"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..541)
                     /locus_tag="ACGEXS_001807"
     CDS             complement(<1..541)
                     /locus_tag="ACGEXS_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HFF8012807.1"
                     /translation="MLGTDELYKVLYEHLGPQFWWPADNDIEMMLGAILVQNTRWRNA
                     EIALNQIKEHTHFNPNHILELPIETLQSLIHSSGFYKSKSLTIKTLLTWLARHHFNYQ
                     EINERYKGGLRKELLSLKGIGSETADVLLVYIFGRIEFIPDSYTRKIYDKLGYENTKN
                     YDQLKKVVTLPNHFTNQDAN"
     gene            complement(930..2591)
                     /gene="argS"
                     /locus_tag="ACGEXS_001808"
     CDS             complement(930..2591)
                     /gene="argS"
                     /locus_tag="ACGEXS_001808"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFF8012808.1"
                     /translation="MNIIDQVKQTLVEEIAASINKAGLADEIPDIKIEVPKDTKNGDY
                     ATNIAMVLTNIAKRNPREIAQAIVDNLDTEKAHVKQIDIAGPGFINFYLDNQYLTAII
                     PEAIEKGDQFGHVNESKGQNVLLEYVSANPTGDLHIGHARNAAVGDALANILTAAGYN
                     VTREYYINDAGNQITNLARSIETRFFEALGDNSYSMPEDGYNGKDIIEIGKDLAEKHP
                     EIKDYSEEARLKEFRKLGVEYEMAKLKNDLAEFNTHFDNWFSETSLYEKGEILEVLAK
                     MKELGYTYEADGATWLRTTDFKDDKDRVLIKNDGTYTYFLPDIAYHFDKVKRGNDILI
                     DLFGADHHGYINRLKASLETFGVDSNRLEIQIMQMVRLMENGKEVKMSKRTGNAITLR
                     EIMDEVGVDAARYFLTMRSPDSHFDFDMELAKEQSQDNPVYYAQYAHARICSILKQAK
                     EQGIEVTAANDFTTITNEKAIELLKKVADFEPTIESAAEHRSAHRITNYIQDLASHFH
                     KFYNAEKVLTDDIEKTKAHVAMIEAVRITLKNALAMVGVSAPESM"
     gene            complement(2588..3004)
                     /locus_tag="ACGEXS_001809"
     CDS             complement(2588..3004)
                     /locus_tag="ACGEXS_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFF8012809.1"
                     /translation="MDKKVSIQTKQVLKQHNEKEKFEFTTEGTWQQRQSNFIRYVEQI
                     EDATVNVTIKVDDDSVKLIRKGDINMNLHFVEGQTTTTFYDISAGRIPLEVKTLRILH
                     FVSGDGGKLKIHYELYQDNEKMGSYQYEINYKEIGE"
     gene            complement(3097..3204)
                     /locus_tag="ACGEXS_001810"
     CDS             complement(3097..3204)
                     /locus_tag="ACGEXS_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012810.1"
                     /translation="MKDSENLVFFGFEVLFVFYKMAYIYEALIKYGIVN"
     gene            complement(3279..4289)
                     /gene="adhP"
                     /locus_tag="ACGEXS_001811"
     CDS             complement(3279..4289)
                     /gene="adhP"
                     /locus_tag="ACGEXS_001811"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFF8012811.1"
                     /translation="MRAAVVTKDHKVSIEDKKLRALKPGEALVQTEYCGVCHTDLHVK
                     NADFGDVTGVTLGHEGIGKVIEVAEDVESLKIGDRVSIAWMFESCGRCEYCTTGRETL
                     CRSVKNAGYTVDGAMAEQVIVTADYAVKVPEKLDPAAASSITCAGVTTYKAVKVSNVK
                     PGQWLGVFGIGGLGNLALQYAKNVMGAKIVAFDINDDKLAFAKELGADAIINSKDVDP
                     VAEVMKLTDNKGLDATVVTSVAKTPFNQAVDVVKAGARVVAVGLPVDKMNLDIPRLVL
                     DGIEVVGSLVGTRQDLREAFEFAAENKVTPKVQLRKLEEINDIFEEMENGTITGRMVI
                     KF"
     gene            complement(4788..5309)
                     /locus_tag="ACGEXS_001812"
     CDS             complement(4788..5309)
                     /locus_tag="ACGEXS_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwhD family protein"
                     /protein_id="HFF8012812.1"
                     /translation="MEVISLSEKKGFNFNIIKNDPLDGHKGTNIGSISLDNIAPVFID
                     VANKEAFIDIGGMHARAKVEKGVKWITDKAAVEGDEAKEYWLCWVTTERNEQGPYYAG
                     LTACYLLVNKAIRRGYKSMPEHVNMMDKSMKHHIIIDQIGDENKAILKDFLMNHDEGM
                     WKHSSDALHQAFN"
     gene            complement(5337..6632)
                     /locus_tag="ACGEXS_001813"
     CDS             complement(5337..6632)
                     /locus_tag="ACGEXS_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFF8012813.1"
                     /translation="MTNAYVDLKLVEEKVFKDPIHRYIHVEDQLIWDLIKTKEFQRLR
                     RIRQLGTLYLSFHTAEHSRFGHSLGVYEIVRRLIDESFIGHDAWDNKDRPLALCAALL
                     HDLGHGPFSHSFEKIFNTDHEAYTQAIITGDTEVNAVLRKVAPEFPREVAEVINKTHH
                     NKLVISMISSQIDADRMDYLQRDAYFTGVSYGAFDMERILRLMRPSKDEVLIKESGMH
                     AVENFIMSRYQMYWQIYFHPVSRGGEVLLNNCLKRAKQLYNEGYEFKLHPHDFIPFFE
                     ETVTIEQYVELDEAVVTYYLEKWTKEDDAILSDLASRFINRDLFKYIPFDGSIITISE
                     LQELFEAGGINPDYYFVSEAFSDLPYDYDRPGSNRKPIHLLRQDGTIREISNQSLVIH
                     SITGINRQDYKLYYPREMVAKIKDKTIREAIENLINELN"
     gene            complement(6672..7148)
                     /locus_tag="ACGEXS_001814"
     CDS             complement(6672..7148)
                     /locus_tag="ACGEXS_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFF8012814.1"
                     /translation="MFKVRQATEKDVVQIRDVATKAWFNTYLNIYAATTVNHLLEASY
                     NEHHLKKRLQEQLFLVVEEGNDIVGFANFIYGEELYLSAHYVKPESQHTGYGTALLNE
                     GLSRFEDKFEGVYLEVDNKNEEAVAYYKEQGFTILRSYEPEMYGEKLDLALMYKAF"
     gene            complement(7317..7853)
                     /locus_tag="ACGEXS_001815"
     CDS             complement(7317..7853)
                     /locus_tag="ACGEXS_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFF8012815.1"
                     /translation="MITVLFGGSRPNGNTAQLTKFALQDLEYQWIDVTQHQFKPIRDV
                     RHTAETITSYDDDYLPILDKILASDTIIFASPVYWYSISAPLKAFIEHWSETLQDKRY
                     PNFKAQMAEKDFRVILVGGDCPKIKAKPAITQMKYSLDFLGATLNGYIIGTAEKPGDI
                     MKDNYALARATEWNSILQ"
     gene            complement(8064..9002)
                     /locus_tag="ACGEXS_001816"
     CDS             complement(8064..9002)
                     /locus_tag="ACGEXS_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFF8012816.1"
                     /translation="MKQFVNLGKSDVEVFPIALGTNAVGGHNLYPNLDEEQGKDVVRQ
                     AINHGINLLDTAYIYGPERSEELVGEVVKEYPREQIKIATKGSHEFDENQEVHQNNQP
                     EYLKQQVENSLKRLQTDYIDLYYIHFPDNNTPKDQAVAALQELKEQGKIKAIGVSNFT
                     LDQLKEANKDGYVDVVQLEYNLLHRENEAVLQYCVDHQITFIPYFPLASGILAGKYDE
                     NTKFSDHRTTRRDFKPGVFEENVRRVKALESIAAAHQTSIANIVLAFYLTRPAIDVII
                     PGAKRAEQVVENIKAADIVLSDDEIQYIDELFPIED"
     gene            complement(9272..>9367)
                     /locus_tag="ACGEXS_001817"
     CDS             complement(9272..>9367)
                     /locus_tag="ACGEXS_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012817.1"
                     /translation="YFVWIAVIMGFFLVNIALIIDKNIHVILKNQ"
BASE COUNT         2949 a         1814 c         1226 g         3378 t
ORIGIN      
        1 cattagcatc ttgatttgta aaatgatttg gtaatgtgac tacttttttt aattgatcat
       61 aatttttagt gttttcatat cctaatttat catatatttt tcttgtatag ctatctggaa
      121 taaattcaat acgtccgaat atataaacaa gtaagacatc tgctgtttca cttccaatac
      181 ctttcaaaga taataattct tttcttaatc cacctttata tcgctcatta atctcttgat
      241 aattgaaatg atgtcgtgct aaccatgtta ataatgtttt aatcgtcagt gatttacttt
      301 tataaaagcc acttgaatgt atcaatgatt gtaacgtttc aataggtagt tctaatatat
      361 gatttggatt aaaatgcgta tgttctttaa tctgattcaa tgcaatttct gcatttcgcc
      421 atctagtatt ttgaactaaa attgcaccta acatcatttc aatgtcatta tcagcaggcc
      481 accaaaattg tggtccgaga tgttcatata aaactttata taattcatca gttcctaaca
      541 tttacaccct ccattttaga gggagtggga cagaaatgat attatcgtaa aatttatttc
      601 gtcgtcccac cccaacttgc attgtctgta gaaattggga atccaatttc tctttgttgg
      661 ggccccgccg gcaaggttga ctagaattga aaaaagcttg ttacaagcga attttcgttc
      721 agtcaactac tgccattata actttgtaga gcatagaaca ttgatttatg tctcaggctc
      781 tcttatgcag ttgtgcagag catccaactg tgttcctctt aaccttattc acatactaag
      841 cattccccaa tatgtcacat acctaaaaaa aacactccaa cacaaagtat ctttcgagaa
      901 actctacgtt ggagtgtata taaatgttct tacattgatt caggtgcgct tacaccgacc
      961 attgccaatg catttttcaa tgtaattctg accgcttcaa tcatagcaac atgtgctttt
     1021 gttttttcaa tatcatctgt taacactttt tcagcattat agaatttatg gaaatgagaa
     1081 gctaaatctt gaatataatt agtaattcta tgtgccgatc tatgctcagc agcactttca
     1141 attgtaggtt cgaaatcagc tacttttttc aacaattcaa tcgctttttc attagtaatc
     1201 gttgtaaaat cattcgcagc agtcacttca ataccttgct ctttcgcttg ttttaaaatt
     1261 gaacaaatac gcgcatgtgc atattgagca tagtaaactg gattgtcttg agattgctct
     1321 ttcgctaatt ccatatcaaa atcaaagtga ctatcaggac tacgcatagt taagaaataa
     1381 cgtgcagcgt caacgccaac ttcgtccata atttctctta atgtaatcgc attaccagta
     1441 cgtttactca tcttcacttc tttaccattt tccattaaac gaaccatttg catgatttga
     1501 atttctaaac gattactatc tacaccaaac gtttcaagag atgctttcaa acgattaata
     1561 taaccatgat gatcagcacc aaataaatcg attaaaatgt cattaccacg ttttacttta
     1621 tcgaagtggt acgcaatatc tggtaagaaa tacgtatatg taccgtcatt tttaattaat
     1681 actctgtctt tgtcgtcttt aaaatcagtt gtacgtaacc atgtagcgcc atcagcttca
     1741 tacgtataac ctaattcttt catttttgct aaaacttcaa gaatttctcc tttttcatat
     1801 aaagatgttt cactaaacca attatcaaaa tgcgtattga actctgctaa atcatttttc
     1861 aatttagcca tttcgtattc tacgcctaat tttctaaatt ctttcaaacg tgcttcttca
     1921 gaataatctt taatttcagg gtgtttctct gctaaatctt tacctatttc aataatatct
     1981 tttccattat agccatcttc tggcattgaa taactattgt cacctaaagc ttcaaagaaa
     2041 cgtgtttcaa tcgaacgcgc taagttagta atttgattac cagcatcatt aatataatat
     2101 tcacgtgtta cattatagcc agctgcagtt aaaatattag ctaaagcatc accaactgct
     2161 gcatttctag catgaccaat atgtaaatct cctgtagggt tagctgaaac atactcaagc
     2221 aatacatttt gaccttttga ttcatttaca tgtccaaatt gatcaccttt ttcaattgct
     2281 tcaggaataa ttgctgttaa atactgatta tctaagtaaa aattaatgaa tcctggacca
     2341 gcaatgtcaa tttgttttac atgtgctttt tcagtatcta agttatcaac aatcgcttga
     2401 gcaatttcac gaggattacg ctttgcaata ttagtcagta ccatcgcaat attagtagca
     2461 taatctccat tttttgtatc tttaggaact tcaattttaa tatcaggaat ctcatctgct
     2521 aatcctgctt tgttaatact tgctgcaatt tcttctacta atgtttgttt cacttgatca
     2581 ataatattca ttcgcctatc tccttatagt taatttcata ttgataagaa cccatttttt
     2641 cattatcttg atataattca taatgaatct ttagcttgcc accgtctcca cttacgaaat
     2701 gtaaaatgcg taatgtttta acttctagtg gaattcgtcc agccgatata tcgtaaaaag
     2761 ttgttgtcgt ttgtccttca acaaaatgca aattcatatt aatgtcgcct ttacgaatca
     2821 acttaacgct atcatcatcc acttttattg taacattaac tgttgcatcc tcaatttgtt
     2881 ctacataccg aataaagtta gattgccttt gttgccaagt tccttcagta gtaaattcaa
     2941 atttttcttt ttcgttgtgc tgtttcaaca cttgctttgt ttgaatactt acttttttat
     3001 ccaatttact actcacctta atcttcgcat aatagaaata ttatagcata aaataaggtc
     3061 atcttgttat gccttcttaa agctaaatag gttcaattaa ttaacaattc catacttaat
     3121 caacgcttca tatatgtaag ccattttata aaatacaaac aacacctcaa aaccaaaaaa
     3181 cacgagattt tcactatctt tcatgaaaac ctcgtgtttg attataatga tagtgttcag
     3241 agcactacct tctttatcta tatagtcagt tgatattttt aaaatttaat aaccattcta
     3301 ccagttatag taccattttc catttcttca aaaatatcat tgatttcttc taattttctt
     3361 aattgaactt taggtgttac tttattttca gcagcaaatt caaacgcttc acgtaagtct
     3421 tgtcttgtac caacaagtga acctactact tcaataccat caagcactaa tcttgggata
     3481 tctaagttca ttttatcaac aggtaaacca acggcaacaa ctcttgcacc agcttttaca
     3541 acatcaaccg cttggttaaa tggcgtctta gcaactgaag ttaccactgt tgcatctaat
     3601 cctttgttat cagttaattt cataacttct gcaactggat caacatcttt agaattaata
     3661 atagcatcag cacctaattc tttcgcgaat gctaatttat catcattgat gtcgaaggca
     3721 acaattttag cccccataac gtttttagca tattgtaaag ctaggttacc taaaccacct
     3781 ataccaaaaa cacctaacca ttgtccaggt tttacattac ttacttttac agctttataa
     3841 gttgtcacac ctgcgcatgt aatagaagac gctgctgctg gatctaattt ttcaggtact
     3901 ttcacagcat agtctgcagt aacaataact tgttcagcca ttgcaccatc tactgtataa
     3961 ccagcatttt tcacactacg gcaaagtgtt tcacgacctg ttgtacaata ttcacatctt
     4021 ccacagcttt cgaacatcca agcgatagac acacggtctc caatttttaa tgattctaca
     4081 tcttccgcaa cttcgatgac tttaccaata ccttcatgac ctaaagtaac gcctgtaaca
     4141 tcaccaaaat cagcattctt aacatgtaaa tcggtatgac aaacgccaca atattccgtt
     4201 tgtaccaacg cttcaccagg ttttaaagct cttaactttt tgtcctcaat acttactttg
     4261 tgatctttcg ttacaactgc tgctctcata ataatgtcct ccttatagtc actccagtat
     4321 gtatgatatt aattggctat tactttcaaa aaagaaaacg aataattata gatgtaattt
     4381 ttatttgtaa tagacgccag attaaaacat actactgtga ccttgttttt agttagatat
     4441 caaccgttaa cttttaacgt ttccctagtt aatatgttaa aagaaatgac ataatcgata
     4501 tgctaacgtg taatttttac ttcacatata tttatttaaa gataatttat aatactctta
     4561 tcttcactct tattataatg tgaattaatt cacaaagtat atagctatga tcaaaaaaat
     4621 gtgtcagtta ttcaaatttt ttattaacta cagattaatt taatttcaaa aaaattaaaa
     4681 aaactcccaa tcaatctaag acattatttt cacctcacga tttacgtcgt cattgagaaa
     4741 tgaatcatat ctttatagat taattgggaa attttagttt ctaatattta attaaatgct
     4801 tgatgtaaag catcagaaga atgcttccac ataccttcat catggttcat taaaaagtct
     4861 tttaaaatag ctttattctc gtcaccaatt tgatctatga taatatgatg tttcattgat
     4921 ttatccatca tattaacatg ttcaggcata cttttataac cacgacgaat tgctttattc
     4981 actaataaat agcacgctgt taaaccagcg taatatggtc cttgttcatt acgttctgtt
     5041 gttacccaac acaaccaata ttctttagct tcatcgcctt caacagcagc tttatcagta
     5101 atccatttca cacctttttc aactttggca cgagcatgca tgcctccaat atcaataaat
     5161 gcttctttgt tagcaacatc gataaaaact ggtgcaatat tgtctaagct aattgaacca
     5221 atatttgtac ctttatgacc atctagaggg tcatttttta tgatattaaa attaaagcct
     5281 tttttctcag acaatgatat aacctccata tttaacgata acaattttag ccctgtttaa
     5341 ttaagctcat taatcaaatt ttcaatagct tctctaattg tcttatcttt aatctttgca
     5401 accatttctc taggataata taatttatag tcttggcgat taatgcctgt aatactatga
     5461 atgactaatg attgattgct tatttctcta atcgtaccat cttgtcttaa taaatgaatc
     5521 ggtttgcgat ttgaccccgg tcgatcatag tcatatggca aatcagaaaa tgcttcactc
     5581 acaaaataat aatctggatt aataccacct gcttcaaaca gttcttgcag ttctgatatt
     5641 gtaataattg agccatcaaa tggaatatat ttaaataagt ctcgattaat aaatcgactt
     5701 gctaaatcac ttaaaatagc atcatcttct tttgtccatt tttccaaata atacgtaact
     5761 accgcttcat cgagttcaac atattgttca atcgtaactg tctcttcaaa aaatggaata
     5821 aaatcatgtg gatgcaactt aaattcatag ccttcattat aaagctgttt tgcgcgtttc
     5881 aaacaattat taagcagcac ttctccacca cgactaactg ggtggaagta aatttgccaa
     5941 tacatttgat aacgactcat aataaagttt tcaactgcat gcataccact ttctttgatt
     6001 agtacttcat ctttagaagg tcgcattaat cttaaaatac gctccatatc aaaagcacca
     6061 tatgatacac ctgtgaaata cgcatcacgt tgtaaataat ccattctatc cgcatcgatt
     6121 tgtgacgaaa tcatcgaaat gaccaattta ttatgatgcg ttttattaat tacttccgca
     6181 acttctcttg gaaactcagg cgccacttta cgtaatacag cattcacctc agtatctcca
     6241 gtaataatcg cttgtgtgta tgcttcatgg tctgtattaa atattttttc aaaactatgt
     6301 gaaaatggac catgtcctaa atcatgtaat aatgcagcac ataatgccaa cggtctatct
     6361 ttattgtccc atgcatcatg accaataaat gactcatcaa ttaatcgtct aactatttca
     6421 tacacaccta aagaatgtcc aaagcgacta tgttctgctg tgtgaaaaga taggtacagt
     6481 gttcctagtt gtctaattcg acgtaacctt tggaattcct tagttttaat taaatcccat
     6541 atcaattgat cttcaacatg aatatatcga tgtatcgggt ctttaaaaac tttttcttct
     6601 actaatttta aatctacata tgcgttagtc attattcccc tccttttcgt ttaatataat
     6661 atttaattta cttaaaatgc tttgtacata agtgctaagt ctaacttttc gccatacatt
     6721 tctggctcat aagagcgtaa gattgtaaaa ccttgctctt tatagtaagc tactgcttct
     6781 tcatttttat tatctacttc taagtaaaca ccttcaaatt tatcttcaaa acgtgataat
     6841 ccttcattta acaatgctgt accataacct gtatgttgcg attctggttt aacataatga
     6901 gctgataaat ataattcttc accgtaaata aagttagcaa agccaacgat gtcattacct
     6961 tcttcaacga ctaagaataa ttgttcttga agtcttttct ttaaatgatg ttcattatat
     7021 gaagcttcta acaagtgatt aactgttgtc gcagcgtata tatttaagta tgtattaaac
     7081 caagctttag ttgcgacatc tctaatttga acaacatctt tttcagttgc ttgtcttacc
     7141 ttgaacatga ctttctcccc ttattaacaa gttttaataa cggcattata ccacaacttg
     7201 ctcaatactt aataaacaat gattgtctat tcaatttata tatttatatt ttccgttaaa
     7261 attaaaaata aaaaataacg aagcaaaaaa tcacttcgtt tagtatgagg tatgtcttat
     7321 tgcaatatac tattccactc agttgcacgt gctaaggcat agttgtcttt catgatgtca
     7381 ccaggctttt cagcagttcc aataatataa ccatttaaag tggcacctaa aaagtctaaa
     7441 ctatatttca tttgcgtaat tgctggcttc gcttttattt ttggacaatc tccaccaact
     7501 aaaataactc taaaatcctt ttcggccatt tgtgccttaa aattaggata tcgtttatct
     7561 tgtaatgttt ctgaccaatg ttcgataaat gctttcaatg gtgctgaaat gctataccaa
     7621 tacactggtg atgcaaaaat aattgtatca ctagccaata ttttatctag aatcggcaaa
     7681 tagtcatcgt catatgaagt aatagtctct gctgtatgtc tcacgtcacg tatcggttta
     7741 aactgatgtt gtgtcacgtc aatccattga tactctaaat cttgcaaagc gaattttgtt
     7801 aattgtgcag tattaccgtt tggtctactc ccaccaaaca aaacagtaat cattttagcc
     7861 taacctcact tttgattaat aaatatctgt gtttttcgtt acctaattat actatcataa
     7921 gctttgccta ccgaatagta aaacgcttac aacttttata taaatttgac gaaatttcgt
     7981 catgccttat ataacgtcgt ttgtgatacg gggctaattc atgatgaaat tagatacata
     8041 tatcaccatt aaatacaatt catttagtct tcaatcggaa acagttcatc gatatattga
     8101 atctcatcat ctgataaaac gatatctgca gctttaatat tttcaacgac ttgttctgca
     8161 cgttttgcac caggaataat cacatcgata gctggtctcg ttaaataaaa tgctaataca
     8221 atgttcgcaa ttgaagtttg atgtgctgca gctatgcttt ccaaagcttt tacgcgacgc
     8281 acattttctt caaatacacc tggtttaaaa tcacgacgtg tagtacgatg gtcactaaat
     8341 ttagtgttct catcatattt tccagctaaa ataccggatg ctaatgggaa atatggaata
     8401 aatgtgattt ggtgatcaac acaatattgt aatactgcct cattttcgcg atgcaataaa
     8461 ttatattcta actgtacaac atcaacgtaa ccatctttat ttgcttcttt aagttgatct
     8521 aatgtgaaat ttgatacacc aattgcttta atcttccctt gttccttaag ctcttgtaat
     8581 gctgcaactg cttgatcttt cggagtgttg ttatccggaa aatgaatata atataaatcg
     8641 atataatcag tttgtagacg tttcaaacta ttctcaactt gttgttttaa atattccggt
     8701 tgattgttct gatgtacttc ttgattttca tcaaattcat gagacccttt cgtagcaatt
     8761 ttaatttgct ctcgcggata ttctttaaca acttctccaa ccaattcttc tgatcgttct
     8821 ggcccataaa tatatgccgt atctaataaa ttaataccat gattaatggc ttgacgaaca
     8881 acatcttttc cttgttcttc atctaagttc ggatataaat tatgcccacc tactgcgttc
     8941 gtcccaagtg cgattggaaa cacttcaaca tcagatttac ctaagtttac aaattgcttc
     9001 attagaccca gctccttatc tttataactt ttccactatc acactatcat gatttaatac
     9061 aacttccaaa tgttagaatt agagcttgaa tttaagcggt gtaataggaa aacgttatat
     9121 aaaactcagt taaaataagt attgcttaaa ttgatatgca tatagcgcga ttgctcgttg
     9181 acctttaaat tacatgtact tttgaatcag aaattgaaaa acatttaaga acgacaatat
     9241 taaagccatt gcaaatttaa tttggtaatt tctattggtt tttaagtatg acatgaatgt
     9301 ttttatctat aattaaagct atattaacta gaaaaaaacc cattataact gcaatccaaa
     9361 caaaata
//
LOCUS       DAXWFK010000100         9348 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.100, whole genome shotgun sequence.
ACCESSION   DAXWFK010000100 DAXWFK010000000
VERSION     DAXWFK010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9348
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.100"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            86..550
                     /locus_tag="ACGEXS_001818"
     CDS             86..550
                     /locus_tag="ACGEXS_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8012818.1"
                     /translation="MDRQSFTDLIQTKFKMVRIEAGYTQDTMAQTIGLSKKTLVQIEK
                     ERVLPNWTTCISICALFRDSEVLNSTFGCDPLEIVQTISRNHCAYPNHAPTSDIYWNN
                     IETRNGYILQSNKVSNIYRVLNPDNQPIFGTSKMREAETYFNRNAKEELVHI"
     gene            729..1853
                     /locus_tag="ACGEXS_001819"
     CDS             729..1853
                     /locus_tag="ACGEXS_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFF8012819.1"
                     /translation="MNALFIIIFMIVVGAIIGGITNVIAIRMLFHPFKPYYIFKFRVP
                     FTPGLIPKRREEIATKIGQVIEEHLLTETLINEKLKSEQSQQAIESMIQQQLQKLTKD
                     QLSIKQITSQIDIDLEQVLQTNGNQYIESQLNNYYTKHQNQTIASLLPNQLVTFLNQH
                     VDNATDLLCDRARNYLSSAKGTQDINDMLDTFFNEKGKLIGMLQMFMTKESIADRIQQ
                     ELIRLTSHPKARTIVTSLITNEYQTFKDKPLNELLDASQFNEIAENLSVYVTTYASKQ
                     ANKPVVTLMPQFVDYLEGQLSSKLANLIIEKLSIHLSTIMKKVDLRGLIEEQINTFDL
                     DYIEKLIIEIANKELKLIMSLGFILGGIIGFFQGLVAIFV"
     gene            1922..2266
                     /locus_tag="ACGEXS_001820"
     CDS             1922..2266
                     /locus_tag="ACGEXS_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002498160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HFF8012820.1"
                     /translation="MAVNLYDYANQLEQALRESEEYKAIKEAFANVKANEESKKLFDE
                     FRETQINFQQKQMQGEEIAEEDLQKAQEQAQAIEKDENISALMNAEQKMSQVFQEINQ
                     IIVKPLDEIYAD"
     gene            2649..2747
                     /locus_tag="ACGEXS_001821"
     CDS             2649..2747
                     /locus_tag="ACGEXS_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012821.1"
                     /translation="MPFDSLAVLITTEIAFPTEIFEKYQRTLQIKF"
     gene            3038..4234
                     /locus_tag="ACGEXS_001822"
     CDS             3038..4234
                     /locus_tag="ACGEXS_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFF8012822.1"
                     /translation="MVKFIHCSDLHLDSPFKSKSHISPKIFEDVQKSAYESFKNIVDI
                     ALQQDVDFVIIAGDLFDSENRTLRAEIFLKQQFERLQNEQIFVYVCHGNHDPLSSKIS
                     SNWPDNVSVFSNKVETYEAITKSGETIYIHGFSYENRASYENKIDEYPSSQGQKGIHI
                     GVLHGTYSKSSVNERYTEFILEDLNSKLYHYWALGHIHERQQLSDMPVINYSGNIQGR
                     HFNEQGEKGCLLIEGDHLKLKTKFYPTQYIRFEEATIETDKTSKQGLYEVIQNFKEQV
                     REEGKAFYRLTLVINSETLISPQDLLQVEEMITDYEENENQFVYIDELKIQYAQNDES
                     PLVNEFSAELLVDQTVFDKAMSDLYLNPRASKFLDDYGTFDHTALVNRAEEILKAEMR
                     GEQNDN"
     gene            4224..7160
                     /locus_tag="ACGEXS_001823"
     CDS             4224..7160
                     /locus_tag="ACGEXS_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8012823.1"
                     /translation="MIIKSLEIYGYGQFVQRKIEFNKNFTEIFGENEAGKSTIQAFIH
                     SILFGFPTKKSKEPRLEPRLGNQYGGKLVLILDDGLEIEVERIKGSAQGDVKVYLPNG
                     AVRDDAWLQKKLNYISKKTYQGIFSFDVLGLQDIHRNLNEKQLQDYLLQAGALGSTEF
                     TSMREVINRKKDELYKKSGKNPIINQQIEQLKQLESQIREEEAKLETYHRLVDDRDKS
                     SRRLENLKHNLNQLSKMHEEKQKEVALHDHSQEWKSLEQQLNIEPITFPEKGVDRYEK
                     ARAHKQSLERDIGLRNERLAQLKEEATQLEPVKQSDIDAFISLNQQENEIKNKEFELT
                     AIEKDIANKQRDKDELQSNIGWSETHHDVDSSEAMKSYVSEQIKNKQEQAAYIKQLER
                     SLEENKIEDNAVHSELDSVEEKIVPEETFEKKKEYSQQVIELNEKENLYSKLKERFEI
                     ERQEKQKRQKLLRTTFILLTLVGIGLTAFSFISNNMLFGIIFAVLTLVFVIGIIMSKS
                     KEVDYSEAITDEIEEIKAQLAILDENYDLDFDLDEQYRIRDHWQQALKNKDILEEKRQ
                     YIEGRLNDAKGRHDELQSTVENVKDELYLSSKISNDLIVDSISTMANIKALDQHISDL
                     NQQRQQLVQELDTFYNHAEAVTKSQFVYFNKLSLFHDVQQWLKSAEDTNEKWRINAEN
                     TKLVTNELNHLNAQLEENNKEITALFDFINVGTEEDFYQHHEDYQTYTSNLSRFNDLT
                     KYLENQNYSYELSSSLSEKTTAQLEEEDHLLATQVDEYNEQYLEMQAQVSDLSAQINH
                     METDTTLANLRHEYHSLKNQLNDIAKDWASLSYLQSLVDEHIKQIKDKRLPQVINEAV
                     EILKHLTDGRYTMINYNEDSITVKHVNGQLYDPVELSQSTKELLYVALRISLIKVLRP
                     YYPFPLIVDDAFVHFDKKRTEKMLNYLRSLSEHYQVLYFTCVKDNIVPSKEVITLNKI
                     EEGGKR"
     gene            7157..8098
                     /gene="yhaM"
                     /locus_tag="ACGEXS_001824"
     CDS             7157..8098
                     /gene="yhaM"
                     /locus_tag="ACGEXS_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM"
                     /protein_id="HFF8012824.1"
                     /translation="MRNIENLNPGDSVDHFFLVHKATQGVTAQGKDYMTLHLQDKSGE
                     IEAKFWTATKNDMATIKPEEIVHVKGDIINYRGNKQMKVNQIRLATTEDQLKTEQFVD
                     GAPLSPAEIQEEISHYLLDIENANLQRITRHLLKKYQERFYTYPAASSHHHNFASGLS
                     YHVLTMLRIAKSICDIYPLLNKSLLYSGIILHDIGKVRELSGPVATSYTVEGNLLGHI
                     SIASDEVVEAARELNIEGEEIMLLRHMILSHHGKLEYGSPKLPYLKEAEILCYIDNID
                     ARMNMFEKAYKKTDKGQFTDKIFGLENRRFYNPESLD"
     gene            complement(8219..9181)
                     /locus_tag="ACGEXS_001825"
     CDS             complement(8219..9181)
                     /locus_tag="ACGEXS_001825"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFF8012825.1"
                     /translation="MKMINKLIVPVTASALLLGACGASATDSKENTLISSKAGDVTVA
                     DTMKKIGKDQIANASFTEMLNKILADKYKNKVNDKKIDEQIEKMQKQYGGKDKFEKAL
                     QQQGLTADKYKENLRTAAYHKELLSDKIKISDSEIKEDSKKASHILIKVKSKKSDKEG
                     LDDKEAKQKAEEIQKEVSKDPSKFGEIAKKESMDTGSAKKDGELGYVLKGQTDKDFEK
                     ALFKLKDGEVSEVVKSSFGYHIIKADKPTDFNSEKQSLKEKLVDQKVQKNPKLLTDAY
                     KDLLKEYDVDFKDRDIKSVVEDKILNPEKLKQGGAQGGQSGMSQ"
BASE COUNT         3482 a         1324 c         1583 g         2959 t
ORIGIN      
        1 atgtaatctg tgtttcaaga aatgtgtatt attgcaattt cttgtgtccg gataaaattc
       61 cccaaacaga aaggtagata acagaatgga tagacagagt tttacagatt taattcaaac
      121 aaaatttaaa atggttcgta tagaggctgg ttatacgcaa gatactatgg cgcaaacaat
      181 tggactttct aaaaagactt tagtacaaat cgaaaaagag agagtattac caaactggac
      241 aacttgtatt tcaatttgtg cattattcag agactctgaa gtattaaaca gtacatttgg
      301 ctgtgatcca ttagaaatcg ttcaaacaat ttcaagaaat cactgtgcat atccaaacca
      361 tgcaccaaca agtgatattt attggaataa cattgagact cgtaatgggt acattttaca
      421 aagtaacaaa gtaagcaata tttatcgtgt attaaatcca gataaccaac caattttcgg
      481 tacttctaaa atgagagaag ctgaaacata ctttaacaga aatgctaaag aagaattagt
      541 acatatctag gcctatatac ttttaataaa taataaaagc gatgtaacac acctgtttcg
      601 agaggtgagg catcgctttt tgtatgtaaa ctaataataa attgagcaga aagtgaaatg
      661 attaatgaat taaaaaataa aaatagtggt ataatgaata actatgttgt cacaaaggag
      721 tcaaaattat gaatgcacta tttattatca tatttatgat tgtagtaggg gcgataattg
      781 gtggcattac taatgtaatt gcaattagaa tgctgtttca cccctttaaa ccatactata
      841 tatttaaatt tagagtccca tttacaccag gtttaatacc gaaaagacgc gaagaaattg
      901 caactaaaat tggccaagtg attgaagagc atttgcttac agaaacttta attaatgaaa
      961 aattgaaaag cgagcaatca cagcaagcaa tagaatctat gattcaacag cagttacaaa
     1021 agttgacgaa agatcaattg tcaataaaac aaattacttc tcaaatcgat attgatttag
     1081 aacaagtatt acaaactaat ggaaatcaat atattgaatc acaattgaat aattattata
     1141 caaagcatca aaaccaaaca atagcatctt tattgccaaa tcaacttgta acatttttaa
     1201 atcagcatgt agataacgca acagacttat tatgtgatcg tgcaaggaat tatttatcat
     1261 ctgcaaaagg cacacaagat attaatgata tgttggatac ttttttcaat gagaaaggta
     1321 agttaattgg tatgttgcaa atgtttatga caaaagagag tattgcagat cgcattcaac
     1381 aagaacttat acgtttaaca tctcatccta aagcaagaac aattgtgaca tcgttaatta
     1441 ccaatgaata tcaaactttt aaagataaac cattgaatga actgttagat gcatcgcagt
     1501 ttaatgaaat cgctgaaaat ctatcagtgt atgtaacaac atatgcaagc aaacaagcga
     1561 ataagccagt ggttacgtta atgcctcaat tcgttgatta tttagaaggt caactctcta
     1621 gcaaattagc aaatttaatc attgaaaaat tatcgattca cttatctaca attatgaaaa
     1681 aagtggattt acgaggatta attgaagaac aaattaatac gtttgactta gattatattg
     1741 aaaaattaat tatagaaatt gctaacaaag aactaaagtt gattatgtca ttaggtttca
     1801 ttttaggtgg tattattgga tttttccaag gtttggttgc aatctttgtc taaaaaagcg
     1861 agatgtggta aagtaatatc gaacgtttta ataaacatca caacaaagga gtgctataac
     1921 catggcagta aatttatatg attatgcaaa tcaattagaa caagctttaa gagaaagcga
     1981 agaatacaaa gcaatcaaag aagcattcgc taatgtaaaa gctaacgaag aatctaaaaa
     2041 gttattcgac gagttccgtg aaactcaaat taacttccaa caaaaacaaa tgcaaggtga
     2101 agaaattgct gaagaagatt tacaaaaagc gcaagaacaa gcgcaagcaa ttgaaaaaga
     2161 tgaaaacatc tctgcattaa tgaatgctga acaaaaaatg agtcaagtat tccaagaaat
     2221 caaccaaatt atcgttaaac cattagacga aatttacgct gactaatatt aaaataatat
     2281 ttttcaatcc tttaaataga ggaatagact gccgacagag tccgagactt tgtgggtagt
     2341 ttttttagtt ttgataacgg aagttagagg ctctctgtca aattgggcag atgcattcag
     2401 taacggaagt taggctagta aacgtgtcct aacttccttt ttgcattttg aagtgtaaag
     2461 ttttcaacat aatactatta gttcggtcat gtatcggact gatggaaaag cgtttcactt
     2521 ttaatgactc attaagaacg gcctgaaaat gtttggcgta ttaagtgcaa tgatagtttt
     2581 gacatttagt ttctaattgg tcattactgc cgagcaaatc tagtagagta atcatgtaaa
     2641 tctttaatgt gccatttgat tcactagcgg tgttaataac tacggaaatt gcatttccga
     2701 ctgaaatttt tgaaaaatat caacgtacgc tacaaataaa attttaaact gttataaatg
     2761 tgtctcaatt tcatatgttc atcgacgata tgaagcgtat tatggtaaaa tgaagaaata
     2821 ataaacttgt taataaataa aacatcacga tttgactaaa gcactttatt attgtgtaga
     2881 taatagtttt ttaacgaaat aaaaatggcg actggtttta ataaatcagc taatgaatca
     2941 ctacacctat aagtatgaat atagtgatta gaatgctttg tatagttgga ttttgcaaaa
     3001 ttgatgttag aacctttaat taaggagata tggatgtatg gttaaattta ttcattgttc
     3061 ggatttacat ttagatagtc ctttcaaatc taagagtcat ataagcccta aaatttttga
     3121 ggatgttcaa aaaagtgctt atgaaagttt taaaaatatt gtagatattg cattacaaca
     3181 agatgttgat tttgtaatta tcgcgggtga tttatttgat agtgaaaaca gaacattacg
     3241 tgctgaaatt ttcttaaaac agcaatttga acgtttacaa aatgaacaaa tctttgttta
     3301 tgtttgtcat ggtaatcacg atcctttatc atcaaagatt tcatcaaact ggccagataa
     3361 tgtttctgta ttttcaaata aagttgagac gtatgaagca attactaaat ctggtgaaac
     3421 aatttatatt cacggattta gttatgaaaa tagagcaagt tatgagaaca agattgatga
     3481 atatccatca agtcaaggcc aaaaaggcat acatattggt gtcttgcatg gtacgtatag
     3541 taaatcttca gttaacgaaa gatataccga gttcatttta gaagatttaa acagtaaatt
     3601 gtatcattat tgggctttag gtcatataca tgaacgtcaa caattaagtg atatgcctgt
     3661 aattaactat tcaggtaata ttcaaggtag acattttaat gagcaaggtg aaaaaggttg
     3721 cttattaatc gagggtgacc acttaaaatt aaagactaaa ttttatccta cacagtatat
     3781 tagatttgaa gaagcaacta ttgaaacgga taagacatct aagcaaggtt tatacgaggt
     3841 cattcaaaac tttaaagaac aagtgagaga agaaggaaaa gccttttatc gtttaacgct
     3901 tgttattaat agtgagacat taatttcacc tcaagattta ttacaagttg aagaaatgat
     3961 tacagattat gaagaaaacg aaaatcaatt tgtatatatt gatgagttaa aaatacaata
     4021 tgcacaaaat gatgagtcac ctttagttaa tgaattttca gcggaattat tagtcgatca
     4081 aactgttttt gataaagcga tgtcagattt atatttaaat ccaagggcat ctaagttcct
     4141 agacgattat ggaacattcg accatacagc attagttaat cgtgctgaag aaatattaaa
     4201 agctgaaatg agaggtgaac aaaatgataa ttaaatcact tgaaatttat ggttacggtc
     4261 aatttgttca acgtaaaatt gaatttaata aaaacttcac tgaaattttt ggtgaaaatg
     4321 aagcgggtaa atcgacgatt caagcattca tccattcgat attatttgga tttccaacta
     4381 aaaagtctaa agagccaaga ctagaaccac gtctaggtaa ccaatacggt ggtaaattag
     4441 tacttattct tgatgatggc ttagagattg aagttgaacg aattaaaggc agtgctcaag
     4501 gtgatgtgaa agtatattta cctaatggtg ctgtgcgtga tgatgcttgg ttacaaaaga
     4561 aacttaatta tatttctaaa aagacatatc aaggtatctt ttcatttgat gtactagggc
     4621 ttcaagacat tcatagaaat ctaaatgaaa aacaattgca agattattta ttacaagcag
     4681 gggctttagg atcaactgaa ttcacgtcaa tgcgcgaagt gattaatcgt aaaaaagatg
     4741 aattatataa aaaatcaggt aaaaatccga tcattaatca acaaattgag caattaaaac
     4801 aactagaaag tcaaattcgt gaagaagaag caaagctaga aacatatcat cgcttagtag
     4861 atgatcgaga taaatcatca cgtcgattag agaatttaaa gcataattta aatcaattat
     4921 caaaaatgca tgaagaaaaa caaaaagagg ttgctttaca tgatcattca caagaatgga
     4981 agtctctaga acaacagtta aatattgagc caatcacatt cccagaaaaa ggtgtggatc
     5041 gttacgaaaa agcacgagcg cataagcaat cgttagaaag agatattggt ttaagaaatg
     5101 agcgtttagc tcaacttaaa gaagaagcga ctcaattaga gccagttaaa caatctgata
     5161 ttgacgcctt cattagtttg aatcaacaag aaaatgaaat taaaaataaa gaatttgaac
     5221 ttactgcaat cgaaaaggat attgcgaata aacaacgtga taaagatgaa ttgcaatcaa
     5281 atattggttg gtctgaaacg catcatgacg tagatagttc agaggcaatg aaaagttatg
     5341 tcagtgagca aatcaagaat aaacaagaac aagctgcata cattaaacaa ttagaacgta
     5401 gtttagaaga aaataaaatc gaagataatg cggttcatag cgaactagat tctgttgaag
     5461 aaaaaatagt tcctgaagag acttttgaaa agaaaaaaga atactcacaa caagtcattg
     5521 aattaaacga aaaagagaac ttgtatagca aattgaaaga acgtttcgaa attgagcgac
     5581 aagaaaaaca aaaacgtcaa aaattgttac gaacaacgtt tattctttta acattagtag
     5641 gtattggttt aactgccttc tcatttattt caaacaacat gttgtttggt attattttcg
     5701 cagtattaac tttagtattt gttatcggta tcatcatgtc taaatcaaaa gaagtagatt
     5761 atagcgaggc catcacagat gaaatcgaag aaattaaagc acaactagcc attttagatg
     5821 aaaattatga cttagatttt gatcttgatg agcaatatcg cattcgtgat cactggcaac
     5881 aagctttgaa aaataaagat attttagaag agaaacgtca gtatattgaa ggtcgtttaa
     5941 atgatgcgaa agggcgtcat gatgaattac aatcgacagt tgagaacgtt aaggatgaat
     6001 tatacttatc atctaaaatt tcaaatgatt taattgttga tagtatttct acaatggcga
     6061 atattaaagc attagatcaa catatcagtg atttaaatca acaacgccag caattggttc
     6121 aagaattaga tacattctac aatcatgctg aagctgttac aaaatcacaa tttgtttatt
     6181 tcaacaaatt atctttattc cacgatgtgc aacaatggtt gaaatcagct gaagacacga
     6241 atgaaaaatg gcgtattaat gctgaaaata ccaaacttgt tacaaatgaa ttaaatcatt
     6301 taaatgctca attagaagaa aataataaag aaattacagc actattcgat tttatcaatg
     6361 ttggtactga agaagatttt taccaacatc atgaagatta ccaaacttat actagtaatt
     6421 tgagtcgttt taatgattta actaaatatc ttgagaacca aaactattct tatgaattaa
     6481 gttctagttt aagtgaaaaa acaactgcac aacttgaaga agaagatcat ttattggcta
     6541 ctcaagttga cgaatataat gagcaatatc ttgaaatgca agcacaagtc agtgatttaa
     6601 gtgcacaaat caatcacatg gaaactgata caacgcttgc taatttaaga catgaatatc
     6661 acagtcttaa aaatcaactt aatgatatcg caaaagattg ggcaagttta agttatttac
     6721 aaagtttagt tgatgaacac attaaacaaa ttaaagataa acgtttgcct caagttatta
     6781 atgaagcggt agaaatattg aagcatttaa cagatggcag atatacgatg attaactata
     6841 atgaagattc aattacggtt aaacatgtta atggtcaatt atatgatcct gttgaactaa
     6901 gtcaatctac aaaagaatta ctttatgtag ctttacgtat cagtttaatt aaggtactaa
     6961 gaccatatta tccgttccca ttaattgttg atgatgcatt tgttcatttt gataaaaaac
     7021 gtactgaaaa aatgttgaat tatttacgat cattatcaga acactatcaa gtactatact
     7081 ttacatgtgt aaaagataat attgttccat caaaagaagt gattacatta aacaaaatag
     7141 aggaaggcgg gaaacgatga gaaatataga gaatctaaat cccggagatt cagttgatca
     7201 ctttttctta gtgcataaag ctacacaggg tgtaacagca caaggtaaag attatatgac
     7261 attacatttg caagataaaa gtggtgaaat tgaagcgaaa ttttggacgg ctacaaaaaa
     7321 tgatatggca acaatcaagc ctgaagaaat tgtacatgtt aaaggtgaca tcataaacta
     7381 tcgcggaaat aaacagatga aagtcaacca aattagacta gcgacaactg aagatcaatt
     7441 aaaaacagaa caatttgtag atggtgcacc tttatcaccg gcagaaatac aagaagagat
     7501 ttctcattat ttgctagata ttgaaaatgc taatttacaa cgtatcacac gtcatttatt
     7561 gaaaaaatat caagaacgat tttacacata tccagctgct agttctcatc atcataactt
     7621 tgcgagtggc ttaagctatc atgtattaac gatgttacgt attgcaaaat caatttgtga
     7681 catttatcca ttgttaaaca aaagtttgtt atatagtggt attattttgc atgatattgg
     7741 taaagttaga gaattgagtg gtcctgttgc gacgtcgtat acagtcgaag gtaacttatt
     7801 aggacacatc tcgattgcga gtgatgaagt agttgaagca gctcgtgaat tgaacattga
     7861 aggagaagaa atcatgttgt taagacatat gattttatct catcatggta agttagagta
     7921 tggttctcca aaactgccat acttaaaaga agcagaaatt ttatgctata tcgataatat
     7981 cgatgctaga atgaatatgt ttgaaaaggc atataaaaaa actgacaagg gtcagtttac
     8041 agataaaata tttggtcttg aaaatcgtag attctacaat cctgaatcac tcgattaaga
     8101 attaaaagat atcggacaga tgagtatact gaaacatgag ctcatctgtc cgatttttaa
     8161 tgcgaaaaaa ccgagcggcc gtggtatgat ttttgaacca cggtcgctcg gttttgtgtt
     8221 attggctcat gccggattgt ccgccttgtg cgccaccttg tttaagtttt tcagggttta
     8281 agattttatc ttcgacaact gatttaatat cacgatcttt aaagtcaaca tcgtattctt
     8341 ttaatagatc tttgtatgca tcagtcaata attttggatt tttttgtact ttctgatcga
     8401 ctaatttttc tttcaggctt tgtttttcac tgttaaagtc tgttggttta tcagctttaa
     8461 taatatgata tccaaagctt gatttaacaa cctctgatac ttcaccatct ttaagcttaa
     8521 atagtgcttt ttcaaaatct ttatcagttt gtcctttaag aacataacct aattcgccat
     8581 cttttttagc tgaaccagta tccattgatt cttttttagc gatttcacca aatttacttg
     8641 gatcttttga aacttctttt tgaatttctt cagctttttg tttcgcttct ttatcatcta
     8701 agccttcttt gtcgcttttc ttagatttaa ctttaattaa aatgtgtgaa gctttcttgc
     8761 tgtcttcttt aatttcagaa tcagagattt taattttatc tgatagtaat tctttatgat
     8821 aagcagcagt acgtaaattt tctttatatt tatcggctgt taaaccttgc tgttgaaggg
     8881 ccttttcaaa tttatcttta ccgccgtatt gcttttgcat tttttcaatt tgttcgtcaa
     8941 tcttcttatc attaacttta tttttatatt tatcagctaa aattttattt aacatttcag
     9001 taaatgatgc atttgcaatt tgatctttac cgattttttt cattgtatct gcaactgtta
     9061 cgtctccagc tttagaagaa attaatgtat tttctttaga gtctgtggca ctagcgccac
     9121 aagcgcctaa taataaagca ctagctgtta ccggaacgat taatttgttt atcatcttca
     9181 tagttgaaac tcctttgtaa gttaatcatc gtgaatataa catagtttta tattatcacc
     9241 aatcaataac ccatgacatc agactagagc attaggttta ggggtagaaa ttgatgaaaa
     9301 taaaaaaaca aggtctatat acatttgatt gtaagctatt acatagcc
//
LOCUS       DAXWFK010000101         9251 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.101, whole genome shotgun sequence.
ACCESSION   DAXWFK010000101 DAXWFK010000000
VERSION     DAXWFK010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9251
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.101"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(242..2698)
                     /locus_tag="ACGEXS_001826"
     CDS             complement(242..2698)
                     /locus_tag="ACGEXS_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFF8012826.1"
                     /translation="MLFGRLTERAQRVLAHAQEEAIRLNHSNIGTEHLLLGLMKEPEG
                     IAAKVLESFNITEDKVIEEVEKLIGHGQDHVGTLHYTPRAKKVIELSMDEARKLHHNF
                     VGTEHILLGLIRENEGVAARVFANLDLNITKARAQVVKALGNPEMSNKNAQASKSNNT
                     PTLDSLARDLTVIAKDGTLDPVIGRDKEITRVIEVLSRRTKNNPVLIGEPGVGKTAIA
                     EGLAQAIVNNEVPETLKDKRVMSLDMGTVVAGTKYRGEFEERLKKVMEEIQQAGNVIL
                     FIDELHTLVGAGGAEGAIDASNILKPALARGELQCIGATTLDEYRKNIEKDAALERRF
                     QPVQVDEPSVVDTVAILKGLRDRYEAHHRINISDEAIEAAVKLSNRYVSDRFLPDKAI
                     DLIDEASSKVRLKSHTTPNNLKEIEQEIEKVKNEKDAAVHAQEFENAANLRDKQTKLE
                     KQYEEAKNEWKNAQNGMSTSLSEEDIAEVIAGWTGIPLTKINETESEKLLSLEDTLHE
                     RVIGQKDAVNSISKAVRRARAGLKDPKRPIGSFIFLGPTGVGKTELARALAESMFGDD
                     DAMIRVDMSEFMEKHAVSRLVGAPPGYVGHDDGGQLTEKVRRKPYSVILFDEIEKAHP
                     DVFNILLQVLDDGHLTDTKGRTVDFRNTIIIMTSNVGAQELQDQRFAGFGGSSDGQDY
                     ETIRKTMLKELKNSFRPEFLNRVDDIIVFHKLTKEELKEIVTMMVNKLTNRLSEQNIN
                     IIVTDKAKDKIAEEGYDPEYGARPLIRAIQKTIEDNLSELILDGNQIEGKKVTVDHDG
                     KEFKYDIAEQTSETKTPSQA"
     gene            complement(2712..3719)
                     /locus_tag="ACGEXS_001827"
     CDS             complement(2712..3719)
                     /locus_tag="ACGEXS_001827"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HFF8012827.1"
                     /translation="MTHNIHDNISQWMKSNEETPIVMSSRIRLARNLENHVHPLMYAT
                     ENDGFRVINEVQDALPNFELMRLDQMDQQSKMKMVAKHLISPELIKQPAAAVLVNDDE
                     SLSVMINEEDHIRIQAMGTDTTLQALYNQASSIDDELDRSLDISYDEQLGYLTTCPTN
                     IGTGMRASVMLHLPGLSIMKRMTRIAQTINRFGYTIRGIYGEGSQVYGHTYQVSNQLT
                     LGKSELEIIETLTEVVNQIIHEEKQIRQKLDTYNQLETQDRVFRSLGILQNCRMITME
                     EASYRLSEVKLGIDLNYIELQNFKFNELMVAIQSPFLLDEEDDKSVKEKRADILREHI
                     K"
     gene            complement(3709..4275)
                     /locus_tag="ACGEXS_001828"
     CDS             complement(3709..4275)
                     /locus_tag="ACGEXS_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HFF8012828.1"
                     /translation="MLCENCQLNEAELKVKVTSKNKTEEKMVCQTCAEGHHPWNQANE
                     QPEYQEHQDNFEEAFVVKQILQHLATKHGINFQEVAFKEEKRCPSCHMTLKDIAHVGK
                     FGCANCYATFKDDIIDIVRRVQGGQFEHVGKTPHSSHKKIALKRKIEEKNEYLKKLIE
                     IQDFEEAAIVRDEIKALKAESEVQHDDA"
     gene            complement(4294..4755)
                     /locus_tag="ACGEXS_001829"
     CDS             complement(4294..4755)
                     /locus_tag="ACGEXS_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFF8012829.1"
                     /translation="MHNMSDIIEQYIKRLFEESNEDVVEIQRANIAQRFDCVPSQLNY
                     VIKTRFTNEHGYEIESKRGGGGYIRITKIENKDATGYINHLLQLIGPSISQQQAYYII
                     DGLLDKMLINEREAKMIQAVIDRETLSMDMVSRDIIRANILKRLLPVINYY"
     gene            4913..6127
                     /locus_tag="ACGEXS_001830"
     CDS             4913..6127
                     /locus_tag="ACGEXS_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFF8012830.1"
                     /translation="MHILIGIIGIIFFLALAFLFSSDRKNIRWKYVGILLAIQLIFAF
                     ILLKTTMGIKVIGGISNGFNYLLLKAADGVNFVFGGIQYIDPKQPPFFFSVLLPIVFI
                     SAIIGILQYTKILPLIINVLGFLISKINGMGRLESYNAVAAAILGQSEVFISIKKQLP
                     YIPKQRLYTLTASAMSTVSASIIGAYFTLIEPKYVVTAVVLNLFGGFIIASIINPYKV
                     NEEDDKLLVEETEERQQSFFEMLGEYILDGFKVAVIVGAMLIGYIAIIALLNGIVSNI
                     FSTVSGGAISWDFQTLIGFVFAPFAFLVGVPWQDAVQAGSVMATKLLSNEFVAMQALG
                     KLSDLSEHAKGVTSVFLVSFANFSSIGIISGAIKSLNDKKGDTVARFGLKLLFGATLV
                     SFISAAIAGFFI"
     gene            complement(6335..6895)
                     /gene="pdxT"
                     /locus_tag="ACGEXS_001831"
     CDS             complement(6335..6895)
                     /gene="pdxT"
                     /locus_tag="ACGEXS_001831"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HFF8012831.1"
                     /translation="MKIGVLALQGAVREHIRHIELSGHEGIAVKKVEQLEEIEGLILP
                     GGESTTLRRLMNLYGFKEALQNSTLPMFGTCAGLIVLAQDIVGEEGYLNKLNITVQRN
                     SFGRQVDSFETELDIKGIATDIEGVFIRAPHIEKVGQGVDILCKVNEKIVAVQQGKYL
                     GVSFHPELTDDYRVTDYFINHIVKKA"
     gene            complement(6899..7786)
                     /gene="pdxS"
                     /locus_tag="ACGEXS_001832"
     CDS             complement(6899..7786)
                     /gene="pdxS"
                     /locus_tag="ACGEXS_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011303857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HFF8012832.1"
                     /translation="MSKIIGSDRVKRGMAEMQKGGVIMDVVNAEQARIAEEAGAVAVM
                     ALERVPSDIRAAGGVARMANPKIVEEVMNAVSIPVMAKARIGHITEARVLEAMGVDYI
                     DESEVLTPADEEYHLRKDQFTVPFVCGCRNLGEAARRIGEGAAMLRTKGEPGTGNIVE
                     AVRHMRQVNSEVSRLTVMNDDEIMTFAKDIGAPYEILKQIKDNGRLPVVNFAAGGVAT
                     PQDAALMMELGADGVFVGSGIFKSEDPEKFAKAIVQATTHYQDYELIGRLASELGTAM
                     KGLDINQLSLEERMQERGW"
     gene            7890..>9251
                     /locus_tag="ACGEXS_001833"
     CDS             7890..>9251
                     /locus_tag="ACGEXS_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFF8012833.1"
                     /translation="MKNTLYHQLYEKLKKQIIEGQFKEGDKFYSKRQLSKHLSISQTT
                     VEHAYQLLLDEGYIYSRPRSGYFVSEIESLTILNNQPIPSLFDDDSYKPKASDEAYDY
                     AFNLDEIDTKHFPIELFRKYSKDLYDTNHLNQLRRGHFQGELHLRFQLAFYLFTNRGV
                     ICDPNQIIIGSSTEQLVNQLVDLLYTSTFIIEKPSYPPIKNILDKKQVEYEQIEVEDN
                     GINVDEVIKSQKNIVYITPSHQFPTGYVMDLKKRTQLIQWAQEKEERFIIEDDYDSEF
                     RYFGKPIPAIQGLYSRGEKVIYISTFSKSIFPSCRVAYMVLPYSIMKKYHSQNHIEGN
                     TVPVHMQNLIATFISSGGFERHLNKMRRIYRRKLTYILKRLKPYKEQLDIQGAETGMH
                     FTITVKNGLTLQECLDRANKVKLKLQVYNFDDDQDYKKTPKFILGFGGIDDDALKPHV
                     DALI"
BASE COUNT         2838 a         1734 c         1305 g         3374 t
ORIGIN      
        1 atatagacct attttatttt gacgtaagtt gctagtatct tcaaacaaaa aacctttata
       61 aaaattcata cctttatgct atcgctgtag gctcattaac ttgttacata taattcttaa
      121 ctatcctttg atgattgttt tattagattg tttcgttgat ggatactttc acgaatttct
      181 atagttcaat gctactaaaa aaacagccca aaactttaat ttgttttgga ctgttttata
      241 attatgcttg cgatggtgtt ttagtttctg aagtttgttc agcaatgtca tatttaaact
      301 ctttaccatc atgatctact gtaactttct taccttcaat ttgattacca tctaatatta
      361 attcacttaa attatcttcg atagtttttt gtatcgctct aattaatggt cttgcaccat
      421 attctggatc atatccttct tctgcgattt tgtctttcgc tttatcagtt acaataatat
      481 ttatgttttg ttcagataat cgatttgtta atttattaac catcattgtt acaatttctt
      541 ttaattcttc ttttgttagt ttatggaata caatgatatc atctacacgg tttaaaaatt
      601 ctggacggaa tgaatttttt aattctttta acatcgtttt tcgaattgtt tcataatctt
      661 gtccatcact tgaaccaccg aatccagcaa atcgttgatc ttgtaattct tgtgccccaa
      721 cgtttgatgt cattatgata attgtatttc tgaaatcaac tgtacgtcct tttgtatctg
      781 tcaaatgtcc atcatctaaa acttgtaata gaatattaaa tacatctgga tgagcttttt
      841 caatttcatc aaataaaatt acagaatatg gtttacgtct aactttttca gttaattgtc
      901 caccatcatc atgaccaaca tatcctggag gagcaccaac taatcggctc actgcgtgtt
      961 tttccataaa ttcactcatg tctacacgga tcatcgcatc atcatcgcca aacattgatt
     1021 cagctaaagc tctagctaat tcagttttac caacaccagt tggtccaagg aagataaagc
     1081 taccaattgg tcgtttagga tcttttaacc ctgcacgggc acgtctaacc gctttactga
     1141 ttgaattaac agcatctttt tgcccaataa ctctctcatg taatgtatct tctagactaa
     1201 gaagtttttc agattctgtt tcattgattt tagttaatgg gatacctgtc catcctgcaa
     1261 taacttcagc aatatcttct tctgacaatg aagttgacat gccattttgt gcattcttcc
     1321 attcattttt agcttcttca tattgctttt caagttttgt ttgtttatca cgcaggttag
     1381 cagcattttc aaactcttga gcatgtactg cggcatcttt ttcattttta actttttcaa
     1441 tttcttgttc aatttctttt aaattattag gtgtcgtatg actcttaagt cttactttag
     1501 aacttgcttc atcaattaaa tcaattgctt tatctggtaa gaaacgatct gaaacgtatc
     1561 tgttacttaa tttaacagct gcttcaatag cttcgtctga aatattaata cgatggtgtg
     1621 cttcgtaacg atctcttaat ccttttaaaa tagcaactgt atctactact gaaggttcat
     1681 caacttgtac aggttggaaa cgacgttcta aagccgcgtc tttttcaata tttttgcgat
     1741 attcatctaa tgtagtagca ccaatacatt gtaattcacc acgtgctaat gccggcttca
     1801 aaatattcga agcatcgata gcaccttcag caccaccagc accaactaaa gtatgcaact
     1861 catcaataaa taggatgaca ttacctgctt gttggatttc ttccataacc tttttcagac
     1921 gctcttcaaa ttcaccacga tatttagtac ctgcaactac tgttcccata tctaaagaca
     1981 taacacgctt atcttttaat gtctctggta cctcattatt cactatggct tgcgctaaac
     2041 cttcagcaat agcagtttta ccaacacctg gctctccaat aagcacagga ttgtttttcg
     2101 tacgtctact taatacttca attacacgtg taatttcttt atcacgtcct ataacaggat
     2161 ctaatgtacc gtctttggca atgactgtta agtcacgagc taaactatct aaagttggag
     2221 tattatttga cttactagct tgtgcatttt tattactcat ttcagggttt cctaaagctt
     2281 tcacaacttg tgcacgtgct ttagtaatat ttaaatctag atttgcaaaa actcttgctg
     2341 caacaccttc attttcacga atcaagccta ataaaatatg ttccgttcca acaaaattgt
     2401 gatgtaattt tctagcttca tccatcgata attcaatgac ttttttagct ctaggtgtat
     2461 aatgcaatgt accaacatga tcttgaccat gtccgattaa tttttcaact tcttcaatta
     2521 ctttatcttc agtgatatta aaactttcta atacttttgc agcaattcct tcaggttctt
     2581 tcattaaccc caataatagg tgttctgttc ctatatttga atgatttaaa cgaattgctt
     2641 cttcttgggc atgtgctaat acgcgctgtg cacgctcagt taatctacca aataacataa
     2701 ataatgacct cctactttat atgttctctt agtatatctg ctcgtttttc ttttacagat
     2761 ttgtcatctt cttcatctaa taaaaatggt gactgtatag ctaccatcaa ttcattaaat
     2821 ttaaagtttt gtaattcaat gtaatttaaa tctataccaa gtttaacttc gcttaatcta
     2881 taagaagcct cttccatagt tatcattcta cagttttgta aaatacctag cgagcgaaaa
     2941 acacggtctt gtgtttctaa ttgattataa gtgtctaact tttgtcgtat ttgtttttct
     3001 tcatgaatga tttgattaac aacttctgtt aatgtttcta tgatttctaa ctcagattta
     3061 ccaagtgtaa gttggttgga tacttgataa gtatgtccat aaacttgcga accttcaccg
     3121 taaatacctc tgattgtata tccaaaacga ttaatggttt gagcaatccg tgtcattctt
     3181 ttcataatag atagacctgg caaatgtagc atcacgcttg ctctcatacc agtacctata
     3241 ttggtaggac atgtagttaa ataaccaagt tgttcatcat aacttatatc aaggcttcga
     3301 tctaattcat catcaattga tgaagcttga ttatataaag cctgtaatgt cgtgtcagtt
     3361 cccatagctt gaatacgaat atggtcctct tcatttatca tgacacttaa agattcatcg
     3421 tcattcacta atactgcggc tgctggttgt tttattagtt caggactaat caaatgcttt
     3481 gcaaccattt tcattttact ttgttgatcc atttgatcaa gacgcattaa ttcaaagttt
     3541 ggcaaggcat cttgtacctc atttataact ctaaatccat cattttcagt agcatacatt
     3601 agtggatgca catgattttc taaattacgc gctaaccgaa ttctagaaga cataacaatt
     3661 ggtgtttctt cattactttt catccattgg ctgatattat catgaatatt atgcgtcatc
     3721 atgttgcacc tcactctcag cttttagtgc tttaatttca tctctaacaa tggctgcttc
     3781 ctcaaaatct tggatttcaa taagtttttt caaatattca ttcttttctt cgatttttcg
     3841 ctttaaagct atctttttat gtgaagaatg tggtgtcttt ccaacgtgct caaattgtcc
     3901 accttgaact ctgcggacga tatcaatgat gtcatcttta aatgttgcat aacaattagc
     3961 acacccaaat ttaccaacat gtgcaatatc tttcaaagtc atatgacatg atgggcaacg
     4021 tttttcttct ttaaacgcta cttcttgaaa attaattcca tgtttcgtag ctaaatgttg
     4081 taaaatttgc ttaacaacaa atgcttcttc gaaattatct tgatgttctt gatattcagg
     4141 ttgttcatta gcttgattcc acggatggtg cccctcagca caagtttgac acaccatttt
     4201 ttcttctgtt ttatttttac ttgtaacttt aacttttaat tccgcttcat taagttgaca
     4261 attttcacaa agcacttcaa cacctcattt catttagtaa taatttataa ctggtaacaa
     4321 acgttttaaa atatttgctc taataatatc tctagaaacc atatccattg atagcgtttc
     4381 tctatcaata actgcttgaa tcattttagc ttcacgttca tttattaaca ttttatctaa
     4441 aagcccatca ataatataat aagcttgttg ttgagaaata gaaggtccaa tcagctgaag
     4501 caaatgatta atataacctg ttgcatcttt attttcaatt ttagtgattc ggatgtaacc
     4561 accaccacca cgtttacttt cgatttcata accatgttca ttagtgaatc gtgttttgat
     4621 tacataattt aattgtgatg gtacgcaatc aaaacgctgt gcgatattcg ctctctgaat
     4681 ttcaacgaca tcttcattcg actcttcaaa taaacgtttg atgtattgtt ctatgatgtc
     4741 agacatattg tgcatgtata tcaccccttt ttgaccttct ttgactatat tatatgacct
     4801 attttgacct ttttcaatta atttgttttt atattttcaa aaatttatga tgttagccct
     4861 tccattttat tgtatgatag attgtagaaa aaactaaagg aatgagaaat atatgcatat
     4921 tctaattggg attatcggaa ttatcttctt tttagcactt gcgtttctat ttagctcaga
     4981 caggaaaaat attcgctgga aatatgtagg aattttactt gcaattcagc ttatctttgc
     5041 atttatcttg cttaaaacta caatgggaat caaggttatt ggtggtattt caaacggttt
     5101 taactactta ttattaaaag cagcagatgg tgttaacttt gtattcggtg gtattcaata
     5161 tatcgatcct aagcaaccac cattcttctt cagtgtatta ttacctatcg tatttatttc
     5221 agcgattatc ggtatccttc aatacacaaa gattttacct ttaatcatta atgttttagg
     5281 attcttaatt tctaaaatta atggtatggg acgtttagaa tcatacaatg cagttgctgc
     5341 agcaattttg ggacaatctg aagtatttat ttcaattaaa aaacaattac catacatacc
     5401 taagcaacgt ttatacacat taacagcttc agcaatgtcg actgtttctg cttcaattat
     5461 cggtgcatac tttaccttaa ttgaacctaa atatgttgta acggctgttg tcttaaactt
     5521 atttggtggt ttcatcatcg cttcaattat taacccttat aaagttaatg aagaagatga
     5581 caaattatta gttgaagaaa ctgaagaaag acaacaatca ttcttcgaaa tgctaggaga
     5641 atatatttta gatggattta aagtagctgt cattgtaggc gcaatgttaa tcggatacat
     5701 tgccattatc gctctattaa atggaattgt aagcaatatc tttagtacag tttcaggtgg
     5761 cgctatttct tgggatttcc aaacattaat tggttttgtc tttgcaccat ttgcattctt
     5821 agtcggtgtg ccatggcaag atgcagttca agccggttca gttatggcga caaaactatt
     5881 atctaacgaa tttgtagcaa tgcaagcact tggtaaactt agtgatttat ctgaacacgc
     5941 aaaaggtgtt acatctgtat tcttagtatc atttgctaac tttagctcta tcggtattat
     6001 ttcaggtgcg attaaatcac ttaatgacaa aaaaggagat acagttgccc gctttggttt
     6061 gaaattatta ttcggtgcaa cactagtatc tttcatttca gctgcaatcg ctggattctt
     6121 tatttaacaa atacatcaac atcgaattaa ccccttcaaa attttgacta ttctcagtct
     6181 ctttgaaggg tttttttctt atgtatagct ttatttttta tcatttttaa ttttgacata
     6241 aaaaataact cctccaaagc taagtttgaa ggagatattt tcttctcaac aaatctataa
     6301 atatcaataa ttcgttgatt tagcatacat taagctatgc tttttttaca atatgattaa
     6361 taaagtaatc agttactcta tagtcatctg ttaattcagg atggaatgat acgcctaaat
     6421 atttaccttg ctgaacagct acaattttct cattaacctt acataggata tctacgcctt
     6481 gacctacttt ttcaatatgt ggggctctta taaagacacc ttcaatatct gtagcgatac
     6541 ctttaatatc taattctgtt tcaaagctgt caacttgtct accgaatgag tttcgttgta
     6601 cagtaatatt caacttgtta aggtatcctt cttcaccaac tatatcttgc gctagaacta
     6661 ttaatcctgc gcatgtacca aacataggta aagttgaatt ttgtaaagcc tctttaaatc
     6721 catataaatt cattaatcga cgtaacgttg tagactcgcc accaggtaat attaagccct
     6781 cgatttcttc taattgttca acttttttaa ctgcaatacc ttcatgacca cttaattcaa
     6841 tatgtctaat atgttcacgt actgcacctt gtaatgctaa tacacctatt ttcatatctt
     6901 accaaccacg ctcttgcata cgttcttcta atgataattg attgatatct aaacctttca
     6961 tagcagtgcc aagttcactt gctaatcttc caattagttc atagtcttgg taatgtgttg
     7021 ttgcttgaac aattgcttta gcaaattttt ctggatcttc tgatttaaaa atacctgatc
     7081 caacgaatac accgtcagca cctaattcca tcattaaagc agcatcttga ggagtcgcaa
     7141 cgccaccagc tgcaaagtta actaccggta aacgaccatt gtctttaatt tgttttaaaa
     7201 tttcataagg cgcaccgata tctttcgcaa aagtcataat ctcatcatca ttcattacag
     7261 tcaatcgact aacttctgaa ttaacttgtc tcatatgtct tacagcttca acaatattac
     7321 ctgtacctgg ttcaccttta gtacgtaaca tagcagcacc ttcaccaatt ctacgcgcag
     7381 cttcacctaa attacgacat ccacatacaa atggtactgt aaattgatct tttcttaagt
     7441 gatattcctc atctgctggt gttaacactt ctgattcatc aatatagtca acacccatcg
     7501 cctctaatac tcttgcttca gtgatatgac caatacgtgc tttagccatg actggaatag
     7561 aaacagcatt cattacttct tctacaattt tagggtttgc catacgtgca acaccaccag
     7621 cagctctaat atcagaaggt actcgttcta atgccataac tgctaccgcg ccagcttctt
     7681 ctgcaattct tgcttgctca gcattaacga catccataat aacgccgcct ttttgcattt
     7741 cagccatacc tcttttgact ctgtctgatc caataatttt actcattata cataaaaccc
     7801 cttttcaagt tgattaaata cattgtaatg tataaactga tatttaaaaa ggtacaaaat
     7861 aagataaatt taggaggtca gttataaaat tgaaaaacac tttataccat caactttatg
     7921 aaaaattaaa aaaacaaata attgaaggtc aatttaaaga aggcgataaa ttttattcta
     7981 aaagacaatt aagcaaacat ttatctatta gccaaacaac cgtagagcat gcatatcaac
     8041 ttctattaga cgaaggttac atttattcaa gacctcgctc tggatatttt gtgtccgaaa
     8101 ttgaatcttt aacaatttta aacaatcaac ctatcccctc tctttttgat gatgattcat
     8161 ataaaccaaa agcgagtgat gaagcttatg attatgcttt taatttggat gaaatagata
     8221 cgaaacattt ccctatagaa cttttcagaa agtattccaa agatctttat gatacaaatc
     8281 atttaaatca attacgtaga ggacattttc aaggagaatt acatttgcgg tttcaactgg
     8341 cattttattt atttactaat cgtggcgtaa tttgtgatcc taatcaaatt ataattggct
     8401 catctaccga acaattagta aatcaattag tcgatttact ttatacttca acatttatta
     8461 tcgaaaaacc tagttatcca ccaatcaaaa atattttaga taaaaaacaa gttgaatatg
     8521 aacaaattga agttgaagat aatggaataa acgtagatga agttattaaa tcacaaaaaa
     8581 atatagtata tataacgcct tcccatcaat ttccaactgg atatgtgatg gacttaaaaa
     8641 aacgaactca attaatacaa tgggctcaag aaaaagaaga acgatttatt atagaagatg
     8701 attatgattc cgagtttaga tatttcggca aacccattcc agcaattcaa ggtctatatt
     8761 caagaggaga aaaagtgatt tatatcagta cattttcaaa gtctattttt cctagctgtc
     8821 gagttgcata tatggtgtta ccctattcta taatgaaaaa atatcattct caaaatcaca
     8881 ttgaaggcaa cactgtacct gttcatatgc aaaaccttat tgcaacgttt atatcttccg
     8941 gtggttttga aaggcattta aataaaatga gacgaattta tcgtagaaaa ctaacctata
     9001 ttttaaaaag attgaaacct tacaaagagc agcttgatat tcaaggtgct gaaacgggta
     9061 tgcattttac aattacagta aaaaatggat taacattaca agagtgtctt gatagagcaa
     9121 acaaagtaaa gttaaaatta caagtttata attttgatga tgatcaagac tataagaaaa
     9181 cccctaaatt tatattaggc tttggcggta tagacgatga tgctttaaaa ccgcatgtag
     9241 acgctctaat a
//
LOCUS       DAXWFK010000102         9242 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.102, whole genome shotgun sequence.
ACCESSION   DAXWFK010000102 DAXWFK010000000
VERSION     DAXWFK010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9242
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.102"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            566..748
                     /locus_tag="ACGEXS_001834"
     CDS             566..748
                     /locus_tag="ACGEXS_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012834.1"
                     /translation="MKDSIFWKKAFIPVYFIVAMLVFLLFRFYIKTDNFSIYLMSIFL
                     ICLGTASIIYNYKTNR"
     gene            complement(730..969)
                     /locus_tag="ACGEXS_001835"
     CDS             complement(730..969)
                     /locus_tag="ACGEXS_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012835.1"
                     /translation="MCVVIMRDVGLIDVEDCYDIFGMWCLVNGGLKLSFYGGLKMLFY
                     VGLEFVVLWRYKKPQQDLDLTVTYTFHTYIYRLVL"
     gene            1202..2866
                     /gene="alsS"
                     /locus_tag="ACGEXS_001836"
     CDS             1202..2866
                     /gene="alsS"
                     /locus_tag="ACGEXS_001836"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFF8012836.1"
                     /translation="MTDKKYTAADMVIDTLKNNGVEYVFGIPGAKIDYLFNALIDDGP
                     ELIVTRHEQNAAMMAQGIGRLTGKPGVVLVTSGPGVSNLTTGLLTATSEGDPVLALGG
                     QVKRNDLLRLTHQSIDNAALLKYSSKYSEEVQDPESLSEVMTNAIRIATSGKNGASFI
                     SIPQDVISSPVESKAISLCQKPNLGVPSEQDINDVIEAIKNASFPVLLAGMRSSSAEE
                     TNAIRKLVERTNLPVVETFQGAGVISRELENHFFGRVGLFRNQVGDELLRKSDLVVTI
                     GYDPIEYEASNWNKELETQIINIDEVQAEITNYMQPKKELIGNIAKTIEMISEKVDEP
                     FINQQHLDELEQLRTHIDEETGIKATHEEGILHPVEIIGSMQKVLTDDTTVTVDVGSH
                     YIWMARNFRSYNPRHLLFSNGMQTLGVALPWAISAALVRPNTQVVSVAGDGGFLFSSQ
                     DLETAVRKNLNIIQLIWNDGKYNMVEFQEEMKYKRSSGVDFGPVDFVKYAESFGAKGL
                     RVTNQEELEAAIKEGYETDGPVLIDIPVNYKDNIKLSTNMLPDVFN"
     gene            2903..3607
                     /gene="budA"
                     /locus_tag="ACGEXS_001837"
     CDS             2903..3607
                     /gene="budA"
                     /locus_tag="ACGEXS_001837"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFF8012837.1"
                     /translation="MTNVLYQHGTLGTLMAGLLKGTASINELLQHGDLGIATLTGSNG
                     EVIFLDGKAYHANEHKEFVELKGDELTPYATVTKFVADTSYETKDKSSEAVFAEIKEK
                     MLSENLFSAVKISGLFKKMHVRMMPAQEPPYTRLIDSARRQPEQTETYVKGSVVGFFT
                     PELFHGIGSAGFHVHFANDDRNFGGHVLDFEVEDVKVEIQNIETFEQHFPIQDKDFTK
                     ANIDYKDIADEIREAE"
     gene            complement(3971..4396)
                     /locus_tag="ACGEXS_001838"
     CDS             complement(3971..4396)
                     /locus_tag="ACGEXS_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HFF8012838.1"
                     /translation="MKLKSFVTATLALGLLSTVGAALPSHEASADSNNGYKEMTVDGY
                     HTVPYTISVDGITALHRTYFIFPENKNVLYQEIDSKVKNELASQRGVTTEKINNAQTA
                     TYTLTLNDGNKKVVNLKKNDDAKNSIDPSTIKQIQIVVK"
     gene            4690..5505
                     /locus_tag="ACGEXS_001839"
     CDS             4690..5505
                     /locus_tag="ACGEXS_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HFF8012839.1"
                     /translation="MSKRLLLFDFDETYFKHNTNEEDLSHLREMEKLLEKLTNNNEVI
                     TAVLTGSTFQSVMDKMDQVNMTFKPLHIFSDLSSKMFTWNNGEYVESETYKKKVLSEP
                     FLFEDIEDILRHISAQYNVEFIPQRAFEGNETHYNFYFHSTGNHNNDSRILEALVRYA
                     NDQNYTARFSRSNPLAGDPENAYDIDFTPSNAGKLYATQFLMRKYNIPVKSILGFGDS
                     GNDEAYLSYLEHAYLMSNSRDEALKQKFRLTKYPYYQGITLHVKEFVEGKYDY"
     gene            5716..6570
                     /locus_tag="ACGEXS_001840"
     CDS             5716..6570
                     /locus_tag="ACGEXS_001840"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HFF8012840.1"
                     /translation="MNKTNFGAYLNKSKSRKIYKIFDQNLKREFSYWQFRKLFKSYTR
                     QSDVHELKVCTQNDGTIEQIWIDEANQFGVSVTWKDNSIIGFVMRPLINYDAIKNETT
                     LKYAVPTTDCYEIFWGGDNELYNYHYLYPHQRYAIDLVIKENHKTYKNQGKVNTDYFC
                     FGKDIIAPANGTVEKVVNGVQDNSIGSTNESQFLGNYIVIKHAENEYSLIAHLHQYSI
                     IVNEGQNVKYGDIIGKVGNSGNSTEPHIHFQVMNDKNIEACTSLKIRFINNRELIKGD
                     VVCGLQAE"
     gene            complement(6630..9053)
                     /gene="hysA"
                     /locus_tag="ACGEXS_001841"
     CDS             complement(6630..9053)
                     /gene="hysA"
                     /locus_tag="ACGEXS_001841"
                     /EC_number="4.2.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase HysA"
                     /protein_id="HFF8012841.1"
                     /translation="MTYRIKKWQKLSTITLLMAGVITLNGGEFRSVDKHQIAVADTNV
                     QTPDYEKLRNTWLDVNYGYDKYDENNPDMKKKFDATEKEATNLLKEMKTESGRKYLWS
                     GAETLETNSSHMTRTYRNIEKIAEAMRNPKTTLNTDENKKKVKDALEWLHKNAYGKEP
                     DKKVKELSENFTKTTGKNTNLNWWDYEIGTPKSLTNTLILLNDQFSNEEKKKFTAPIK
                     TFAPDSDKILSSVGKAELAKGGNLVDISKVKLLECIIEEDKDMMKKSIDSFNKVFTYV
                     QDSATGKERNGFYKDGSYIDHQDVPYTGAYGVVLLEGISQMMPMIKETPFNDKTQNDT
                     TLKSWIDDGFMPLIYKGEMMDLSRGRAISRENETSHSASATVMKSLLRLSDAMDDSTK
                     AKYKKIVKSSVESDSSYKQNDYLNSYSDIDKMKSLMTDNSISKNGLTQQLKIYNDMDR
                     VTYHNKDLDFAFGLSMTSKNVARYESINGENLKGWHTGAGMSYLYNSDVKHYHDNFWV
                     TADMKRLSGTTTLDNEILKDTDDKKSSKTFVGGTKVDDQHASIGMDFENQDKTLTAKK
                     SYFILNDKIVFLGTGIKSTDSSKNPVTTIENRKANGYTLYTDDKQTTNSDNQENNSVF
                     LESTDTKKNIGYHFLNKPKITVKKESHTGKWKEINKSQKDTQKTDEYYEVTQKHSNSD
                     NKYGYVLYPGLSKDVFKTKKDEVTVVKQEDDFHVVKDNESVWAGVNYSNSTQTFDINN
                     TKVEVKAKGMFILKKKDDNTYECSFYNPESTNSASDIESKISMTGYSITNKNTSTSNE
                     SGVHFELTK"
     gene            complement(9050..9217)
                     /locus_tag="ACGEXS_001842"
     CDS             complement(9050..9217)
                     /locus_tag="ACGEXS_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012842.1"
                     /translation="MRKIWVIDCLYASTFFVDCLTVDSHLYTLTNFEKLNHYLIIIFI
                     AHAQHKKETCQ"
BASE COUNT         3137 a         1387 c         1558 g         3160 t
ORIGIN      
        1 tacagtaaat aagatcaatt attgaatcca ccgaccagca gattttttta aaaaattaat
       61 accccgttaa taccctttgc ttcaatttga tgaaaatcaa tgaaattcaa aattgaagaa
      121 atcctttaat atcaaggtgt acgacagtct atataatcat gcgaaattct aaaattttct
      181 gaacgtaaaa aaacaaattc ttaaagcagc tcgtcgttca cctcaattct caaaacgtta
      241 attgtcggac gatatatata caaaacacct cgatgttatg tcgaggtgtt tttttgcgtt
      301 tgtgtgggga atatggaata gagtataaat gaattaatgt ctcaggtata gaattaattc
      361 aactatgaat tattgattta cacgtataat ttgtacatgc ttacacaatc aaaagtgtac
      421 attattaaat tgtcatttag cttgcggaga ccgtacgata gtgttctaat aatagatatt
      481 caagggaaag agctatcgga tgataaccag atgataactc aagaaccatg atttaagagt
      541 gttatcaaaa taaggagtga cttaattgaa agattctata ttttggaaga aagcttttat
      601 tcctgtttat tttattgttg cgatgctggt gtttctactt tttaggtttt atattaaaac
      661 agataacttt tctatatatt taatgagtat cttcttaatt tgtttaggaa ctgcttctat
      721 catttataac tataaaacca atcgataaat ataagtatga aatgtataag tcacagttag
      781 atctaagtct tgctgtggct ttttatatct ccataaaacg acaaattcaa gcccgacata
      841 aaacagcatt ttcagcccac cataaaacga caatttcagc ccgccattga ctaaacacca
      901 catcccaaaa atatcgtaac aatcctctac atcaatcaat ccaacatccc tcataatcac
      961 aacgcacaaa atctattcat gcatttttgg aatacttagt attacaaata acgattttta
     1021 ttcatctaac aaaggttata taatgtactg aaggcaattt ttatgtatta caaatctaat
     1081 cgtacatgta aaattttgat aaacatcatt aattttgcgt aactatcatt agattacaaa
     1141 tcacaaagta attacatgta atacacatct atacatcaca tttgaaggga aatgaatata
     1201 aatgactgat aaaaagtaca ctgcagccga tatggttatt gatactttga aaaataatgg
     1261 ggtagaatat gtttttggta ttccgggtgc aaagattgac tatctattta atgctttaat
     1321 tgatgatggt cctgaactta ttgtcactcg tcatgaacaa aacgccgcaa tgatggcgca
     1381 aggtattgga agattaacag gcaaaccggg tgtagtactt gttacaagtg gtcctggtgt
     1441 aagtaattta actactggat tattaaccgc aacatctgaa ggggatcctg tattagcgtt
     1501 aggtggccaa gtgaaacgta atgatttatt acgattaacg catcaaagta ttgataatgc
     1561 tgcgctatta aaatattcat caaaatatag tgaagaagta caagatcctg aatcattatc
     1621 agaagttatg acaaatgcaa ttcgaattgc tacttcagga aaaaatggcg caagttttat
     1681 tagtattccg caagacgtta tttcttcacc agttgaatct aaagctatat cactttgcca
     1741 aaaaccaaat ttaggagtac cgagtgaaca agatattaat gatgtcattg aagcgattaa
     1801 aaatgcatca tttcctgttt tattagctgg tatgagaagt tcaagtgcag aagaaacaaa
     1861 tgccattcgc aaattagttg agcgcacgaa tttaccagtt gtagaaacat tccaaggtgc
     1921 aggtgtaatt agtcgtgaat tagaaaatca tttcttcggt cgtgtgggct tattccgcaa
     1981 tcaagttggt gatgaattat tacgtaaaag tgatttagtt gttacaatcg gttatgatcc
     2041 aattgaatac gaagctagta actggaataa agaattagaa acacaaatta tcaatattga
     2101 cgaagttcaa gctgaaatta ctaattatat gcaaccgaaa aaagagttga ttggtaatat
     2161 tgctaaaacg attgaaatga tttctgaaaa agtggatgag ccatttataa atcaacaaca
     2221 tttagacgaa ttagaacaat taagaacaca tattgatgaa gaaactggta ttaaagcgac
     2281 gcatgaagaa ggaattctac atccagtgga aattattgga tctatgcaaa aggtattaac
     2341 tgatgatact actgtaacag ttgatgttgg aagtcactat atttggatgg cacgtaattt
     2401 cagaagttac aatccaagac atttattatt tagcaatggt atgcaaacgc ttggtgtagc
     2461 attaccgtgg gcaatttcag ctgcacttgt gcgccctaat acgcaagttg tgtccgttgc
     2521 tggcgatggt ggctttttat tttcatcaca agatttagaa acggccgtac gtaaaaattt
     2581 aaatatcatc cagcttattt ggaatgatgg aaaatataac atggttgaat tccaagaaga
     2641 aatgaaatat aaacgttcgt caggtgtaga cttcggtcct gtagattttg taaaatatgc
     2701 agaatcattt ggcgcgaaag gtttacgagt tactaatcaa gaagaattag aagcggcaat
     2761 taaagagggc tatgaaacag atggtccagt attaattgat atacctgtaa attacaaaga
     2821 taatatcaaa ctttcaacaa atatgttacc tgacgtattt aactaaaata aagataaatg
     2881 ttaaagagga gtgggagatt ttatgactaa tgttttatac caacatggta cattaggcac
     2941 attaatggca ggattattaa aaggaactgc atcaataaat gaattattgc aacatggtga
     3001 cttaggtatc gctacactaa caggttcaaa cggtgaggta atctttttag atggaaaagc
     3061 ttaccatgca aatgaacata aagaatttgt agaattaaaa ggtgacgagt taacaccata
     3121 tgcaactgta actaaatttg tagcagatac aagctatgaa acgaaagata aatcttcaga
     3181 agcagttttt gcagaaatta aggaaaagat gttgagtgaa aatttatttt cagcagtaaa
     3241 aatttcaggc ttatttaaaa aaatgcatgt acgtatgatg ccggctcaag aaccacctta
     3301 tacacgttta atcgattcag ctagaagaca acctgaacaa actgaaacgt atgtcaaagg
     3361 ttcagtcgtt ggtttcttta caccagaatt attccatggt atcggatcag caggatttca
     3421 tgtacacttt gcgaatgatg atcgtaactt tggtggacat gtcttagatt ttgaagtaga
     3481 agatgttaaa gtagaaatcc aaaatataga aacatttgaa cagcattttc caattcaaga
     3541 taaagatttc actaaagcaa atattgacta taaagatatt gcagacgaaa ttagagaagc
     3601 tgaataatga atccagaaat ataatgacgg tttatgaaaa ttgacttcat aatgcgcgat
     3661 ttagaaatga tagtttgtaa atatgattaa ccatgactac aatagaacaa atatatttat
     3721 aattacgtct aagtaataaa ataaatccct tcactattag cagtagtgag gggatttatt
     3781 aggttccaga tatttgagat ttgctgttat gtttagatta taaattgtgg tacacactca
     3841 tataaaattt actattgtat aggccaatct gttactacga gaagcaaaca acaataattt
     3901 acaagttcaa taactaaaaa gacaaacgcc aatttttcag cgcttgccct ataaaactat
     3961 tttcaaatta ttatttaact acaatttgta tctgtttgat tgtacttgga tcaattgaat
     4021 ttttagcgtc gtcatttttc tttagattca ctactttttt attaccatca ttcaaagtaa
     4081 gcgtataagt tgctgtttgg gcattattaa ttttttctgt tgtaacacca cgttgagaag
     4141 ctaattcatt ttttacttta ctgtcaattt cttgataaag aacattttta ttttctggga
     4201 agataaagta agttcgatgt aatgcagtaa taccatctac tgaaattgtg taaggaacag
     4261 tgtgataacc atccacagtc atttctttat agccgttatt actatctgca gatgcttcgt
     4321 gactcggtaa tgcagctccg accgttgata ataatcccaa tgctaaagtg gcagtaacaa
     4381 atgattttag tttcatatct caatctcctt ttcgggttgt atgatattac actatttgta
     4441 taacacgttt cataaagttt aaaacatact ttttgcattt tttatatttt aatttttagt
     4501 aatagttttt aacgagggat atgactcgtg cattttgaaa agtggtaagc aatgttttta
     4561 gtttataggg attgattagt tgtatataat aatcatgaaa agcatatagt cgatcttata
     4621 cttttaattg agacttatag tgtatttaag ttatagataa attcattaaa atattatggg
     4681 ggaatgagta tgtcaaagcg tctattattg tttgattttg atgaaacata ttttaaacat
     4741 aatacaaatg aagaagattt aagtcattta agagaaatgg aaaaattgtt ggaaaaacta
     4801 actaataata atgaagtgat cactgctgta ttgacaggaa gtacatttca aagtgtcatg
     4861 gataaaatgg atcaagttaa tatgacgttt aaaccattac atatcttttc tgatttgagt
     4921 tctaaaatgt tcacttggaa taatggcgaa tatgttgaat cagaaacata taaaaagaaa
     4981 gtcttgagcg aaccattttt atttgaagat attgaagata tattacgtca tatttctgcg
     5041 caatataatg tcgaatttat tccacaaaga gcatttgaag gtaatgaaac acattataat
     5101 ttttattttc attcaacagg taatcacaac aatgatagtc gtatcctaga agcacttgtc
     5161 agatacgcaa atgaccaaaa ctatacagcg agatttagtc gaagcaatcc attagctggt
     5221 gatcctgaaa atgcgtatga tattgacttc acaccgtcaa atgctggaaa attatatgct
     5281 actcaatttc tgatgaggaa atataatata ccagtaaaat caatattagg ttttggagat
     5341 agcggcaatg acgaagcata tttaagctat cttgaacatg catatttgat gtcgaatagt
     5401 agagatgaag cattaaagca gaaatttaga ttaacaaaat atccatatta tcaagggata
     5461 acactgcatg ttaaagaatt tgttgaaggt aagtatgatt attgattagt tgagtgatat
     5521 gtatactttt taagtgcaaa tattaatatt taagggatga ttagaaaata tggtgaatcg
     5581 actacacttt agctttaaaa aattatttat atgttgattt atccaattaa gtgtagacga
     5641 ttcaggtagc gcaaaaatga cgcaggaact ggagaaagaa ttgataccaa aattaatata
     5701 ataggttaag tatatatgaa taaaacaaac tttggggctt atttaaataa aagtaagagc
     5761 aggaaaatat ataagatttt tgatcaaaat ttgaagcgag agtttagtta ttggcagttt
     5821 aggaaacttt tcaaatctta tacaaggcag tctgatgtac atgaattaaa agtgtgtact
     5881 caaaatgatg gtactattga gcaaatatgg atagatgaag caaatcaatt tggagtgtca
     5941 gtgacttgga aagataatag tattatcgga tttgttatgc gtccgttaat taattatgat
     6001 gcgattaaga atgaaacgac tttaaaatac gcagtgccga caacagattg ctatgagatt
     6061 ttttggggtg gagataatga attatataat tatcactatt tatacccaca tcaaaggtat
     6121 gctattgatt tagttattaa agaaaatcat aaaacatata agaatcaagg aaaagtaaat
     6181 actgattatt tttgttttgg aaaagatatc attgcgccag caaatggtac agttgaaaaa
     6241 gtagtgaatg gggttcaaga caacagtatt ggaagtacga atgaatcaca gtttttagga
     6301 aattatattg taattaagca cgcagaaaat gagtatagct taatagctca tttacaccaa
     6361 tattcaatca ttgtgaatga ggggcaaaat gttaaatatg gtgatatcat tgggaaggtt
     6421 gggaattctg gcaattctac tgaacctcat atacattttc aagtaatgaa tgataagaat
     6481 attgaagcat gtacatcttt aaaaattcga tttataaata atcgagaact tatcaaaggg
     6541 gatgtggtct gcggattaca agctgaatga tggcgatact tataaaatct cgacactata
     6601 aaaatggtat agtgtcgaga ttttcttgct tatttagtta attcaaagtg cacgccggat
     6661 tcattagaag tcgacgtatt tttgtttgta atagagtaac cggtcattga aattttagat
     6721 tcaatatctg aagcggaatt tgtagattca ggattataga agctacattc ataagtgtta
     6781 tcatctttct ttttaagtat aaacatacct ttggctttaa cttcgacttt agtgttgtta
     6841 atgtcaaaag tttgagtgct attactataa ttaacaccag cccaaaccga ttcattatct
     6901 ttcacaacgt ggaagtcatc ttcttgctta acgacagtta cttcatcttt ttttgtctta
     6961 aagacatctt tagataagcc tggatacaac acatatccat atttattgtc agaattagaa
     7021 tgcttttgag ttacttcata atactcatca gttttttgtg tatccttttg acttttattt
     7081 atttctttcc acttaccagt atgactttct ttttttacag ttattttcgg tttgtttaaa
     7141 aaatgataac cgatgttctt tttggtatct gtggactcta aaaagactga attgttttcc
     7201 tgattatcag aattggttgt ttgtttatcg tctgtatata acgtataccc attcgctttg
     7261 cgattttcaa tcgttgtaac tggattcttt gatgaatcag tacttttaat gccagttcct
     7321 aagaagacaa ttttatcgtt taatatgaaa tatgattttt tggcagttaa agttttgtcc
     7381 tgattttcaa aatccattcc gatactagca tgttggtcat caacttttgt tccgccaaca
     7441 aaagttttac tcgacttttt atcatccgta tcttttaata tttcattgtc taaagttgtt
     7501 gtacctgata aacgtttcat atcggctgtc acccagaagt tatcatgata gtgtttgaca
     7561 tcgctgttat ataaataaga cattccagca ccagtgtgcc aaccttttaa attctctccg
     7621 ttgatacttt catagcgtgc tacgtttttc gacgtcatac ttaaaccaaa tgcaaagtct
     7681 aagtctttgt tatgataggt gacacgatcc atgtcattat atattttaag ttgttgtgtt
     7741 aatccgtttt tagaaatact gttatctgtc attaaagact tcattttatc tatgtccgaa
     7801 tatgaattta aataatcatt ttgtttataa cttgaatctg actctactga agatttgaca
     7861 atctttttat acttagcttt tgttgaatca tccatggcat cacttaatct caacaatgat
     7921 ttcattactg ttgcagatgc tgagtgactg gtttcatttt cacgactgat agctctacct
     7981 cgtgataaat ccatcatttc acctttataa atgagtggca taaatccgtc gtcaatccat
     8041 gactttaagg ttgtatcatt ttgggtttta tcattaaaag gtgtttcttt tatcatcggc
     8101 atcatttgag aaatcccctc taagagtaca acgccataag caccagtgta tggaacgtct
     8161 tgatgatcaa tgtaagagcc atctttataa aatccattac gttctttacc agtggcagaa
     8221 tcttgaacgt aagtgaagac tttattaaat gaatctatag actttttcat catatcttta
     8281 tcttcttcga taatacattc taaaagtttc accttagaaa tgtctactag atttccgcct
     8341 ttagcaagtt cagcttttcc tacagaagat aatattttgt cactatctgg ggcgaaagtt
     8401 ttaatagggg cagtgaattt tttcttttct tcatttgaaa attgatcatt cagcaatata
     8461 agcgtatttg ttaatgattt aggtgttcca atttcataat cccaccaatt taagttggta
     8521 ttcttgccag ttgttttagt aaaattctca cttaattctt ttactttttt atctggttct
     8581 tttccatatg catttttatg cagccactca agggcatctt tcactttctt cttattttcg
     8641 tcagtattta aagtggtttt aggattcctc atcgcttctg cgattttctc aatattacga
     8701 taggtacgag tcatatgaga agaattagtt tcaagggttt ccgctcctga ccacaagtat
     8761 ttcctaccac tttcagtttt catttccttg agtaaattcg tcgcctcttt ctctgtagca
     8821 tcaaacttct tcttcatatc tggattattc tcatcatact tatcataacc atagttaacg
     8881 tccagccatg tgttcctcaa tttttcataa tctggcgttt gaacattcgt atcagccaca
     8941 gcgatttgat gtttatcaac acttctgaat tcaccaccat tcaaagtaat cacaccagcc
     9001 attaataacg taatggtgga taatttttgc catttcttta ttctatatgt cattgacatg
     9061 tctccttttt gtgttgcgcg tgcgcaatga atattatgat taaataatga ttcaattttt
     9121 caaaattcgt taacgtatac aaatgactgt ctactgtcaa acaatccaca aagaatgttg
     9181 atgcatataa acaatcgatc acccaaattt tccgcatcac ccaaaaattg caactaaaat
     9241 aa
//
LOCUS       DAXWFK010000103         8986 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.103, whole genome shotgun sequence.
ACCESSION   DAXWFK010000103 DAXWFK010000000
VERSION     DAXWFK010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8986
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.103"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..694
                     /locus_tag="ACGEXS_001843"
     CDS             <1..694
                     /locus_tag="ACGEXS_001843"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFF8012843.1"
                     /translation="AFENYIKSDEYKGPDMTDFGHRALSDPEFNEQYKEQMKQFKQRI
                     LEDDTFAKQFGDLGNVYGKQWRDWVDKDGNHFDQLKTVIEQIKHNPDSRRHIVSAWNP
                     TEIDTMALPPCHTMFQFYVQDGKLSCQLYQRSADIFLGVPFNIASYALLTHLIAKECG
                     LEVGEFVHTFGDAHIYSNHIDAIQTQLARESFNPPTLKINSDKSIFDINYEDLEIVDY
                     ESHPAIKAPIAV"
     gene            894..1373
                     /locus_tag="ACGEXS_001844"
     CDS             894..1373
                     /locus_tag="ACGEXS_001844"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFF8012844.1"
                     /translation="MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMG
                     RKTFESIGKPLPNRRNVVLTSDTSFNVEGVDVIHSIEDIYQLPGHVFIFGGQTLFEEM
                     IDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK"
     gene            1388..2227
                     /gene="fakB2"
                     /locus_tag="ACGEXS_001845"
     CDS             1388..2227
                     /gene="fakB2"
                     /locus_tag="ACGEXS_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB2"
                     /protein_id="HFF8012845.1"
                     /translation="MTKQIIVTDSTSDLSKEYLEANNIHVIPLSLTIEGASYVDQVDI
                     TSEEFINHIENDEDVKTSQPAIGEFISAYEELGKDGSEIISIHLSSGLSGTYNTAYQA
                     SQMVDANVTVIDSKSISFGLGYQIQHLVELVKEGVSTSEIVKKLNHLRENIKLFVVIG
                     QLNQLIKGGRISKTKGLIGNLMKIKPIGTLDDGRLELVHNARTQNSSIQYLKKEIAEF
                     IGDHEIKSIGVAHANVIEYVDKLKKVFNEAFHVNNYDINVTTPVISAHTGQGAIGLVV
                     LKK"
     gene            2313..2846
                     /gene="msrA"
                     /locus_tag="ACGEXS_001846"
     CDS             2313..2846
                     /gene="msrA"
                     /locus_tag="ACGEXS_001846"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFF8012846.1"
                     /translation="MTKEYATLAGGCFWCMVKPFTSYPGIKSVVSGYSGGHVDNPTYE
                     QVCTNQTGHVEAVQITFDPEVTSFENILDIYFKTFDPTDDQGQFFDRGESYQPVIFYH
                     DEHQKKAAEFKKQQLNEQGIFKKPVITPIKPYKNFYPAEDYHQDYYKKNPVHYYQYQR
                     GSGRKAFIESHWGNQNA"
     gene            2839..3267
                     /gene="msrB"
                     /locus_tag="ACGEXS_001847"
     CDS             2839..3267
                     /gene="msrB"
                     /locus_tag="ACGEXS_001847"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFF8012847.1"
                     /translation="MLKKDKSELTDIEYIVTQENGTEPPFMNEYWNHFAKGIYVDKIS
                     GKPLFTSEEKFHSECGWPSFSKALDDDEIIELVDKSFGMLRTEVRSEESNSHLGHVFN
                     DGPKESGGLRYCINSAAIQFIPYEKLEELGYGDLISHFDK"
     gene            3279..3779
                     /locus_tag="ACGEXS_001848"
     CDS             3279..3779
                     /locus_tag="ACGEXS_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFF8012848.1"
                     /translation="MFKKLFGKGKEVQKDIAIYAPLTGEFVKIEDIPDPVFAQKMMGE
                     GFGINPTEGEVVSPIAGRVDNVFPTKHAIGLKADNGLELLVHIGLDTVQLDGEGFEVL
                     VSSGDEVNVGDPLVRFNLEYINNNAKSVISPIIITNTDQAASINIYDENAVIKGETKV
                     IDVTMN"
     gene            3779..4000
                     /locus_tag="ACGEXS_001849"
     CDS             3779..4000
                     /locus_tag="ACGEXS_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFF8012849.1"
                     /translation="MKNYSFYQFVMTVRGRHDDKGRLAEEIFDDLAFPKHDDDFNILS
                     DYIETHGDFTLPMSVFDDLYEEYTEWLKF"
     gene            4192..5682
                     /locus_tag="ACGEXS_001850"
     CDS             4192..5682
                     /locus_tag="ACGEXS_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HFF8012850.1"
                     /translation="MDDKQHTSSSDDERAEIATSNQDQETNSSKRVHLKRWQFISILI
                     GTILITAVITVVAYIFINQKISGLNKTDQANLNKIENVYKILNSDYYKKQDSDKLSKA
                     AIDGMVKELKDPYSEYLTKEQTKSFNEGVSGDFVGIGAEMQKKNDQIMVTSPMKGSPA
                     ERAGIRPKDVITKVNGKSIKGKALDEVVKDVRGKENTEVTLTVQRGSEEKDVKIKREK
                     IHVKSVEYKKKGKVGVITINKFQNDTSGELKDAVLKAHKDGLKKIVLDLRNNPGGLLD
                     EAVKMANIFIDKGKTVVKLEKGKDTEAIQTSNDALKEAKDMDISILVNEGSASASEVF
                     TGALKDYNKAKVYGSKTFGKGVVQTTREFKDGSLLKYTEMKWLTPDGHYIHGKGIKPD
                     VTIDTPKYQSLNVIPNTKTFKVGDDDKNIKTIKIGLSALGYKVDNESTQFDKALENQV
                     KAFQQANKLEVTGEFNKETNNKFTELLVEKANKHDDVLDKLINILK"
     gene            6082..6591
                     /locus_tag="ACGEXS_001851"
     CDS             6082..6591
                     /locus_tag="ACGEXS_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFF8012851.1"
                     /translation="MIRLGKMSDLDQILNLVEEAKELMKEHDNEQWDDQYPLLEHFEE
                     DIAKDYLYVLEENDKIYGFIVVDQDQAEWYDDIDWPVNREGAFVIHRLTGSKEYKGAA
                     TELFNYVIDVVKARGAEVILTDTFALNKPAQGLFAKFGFHKVGEQLMEYPPYDKGEPF
                     YAYYKNLKE"
     gene            6603..7673
                     /locus_tag="ACGEXS_001852"
     CDS             6603..7673
                     /locus_tag="ACGEXS_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFF8012852.1"
                     /translation="MTKIAFTGGGTVGHVSVNLSLIPTALSQGYEALYIGSKNGIERE
                     MIESQLPEIKYYPISSGKLRRYISLENAKDVFKVLKGILDARKVLKKEKPDLLFSKGG
                     FVSVPVVIAAKSLNIPTIIHESDLTPGLANKIALKFAKKIYTTFEETLNYLPKEKADF
                     IGATIREDLKNGNAHNGYQLTGFNENKKVLLVMGGSLGSKKLNSIIRENLDALLQQYQ
                     VIHLTGKGLKDAQVKKSGYIQYEFVKEDLTDLLAITDTVISRAGSNAIYEFLTLRIPM
                     LLVPLGLDQSRGDQIDNANHFADKGYAKAIDEEQLTAQILLQELNEMEQERTRIINNM
                     KSYEQSYTKEALFDKMIKDALN"
     gene            7690..8304
                     /locus_tag="ACGEXS_001853"
     CDS             7690..8304
                     /locus_tag="ACGEXS_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFF8012853.1"
                     /translation="MSQWKRISLLIVFTLVFGIIAFFHESRLGKWIDNEVYEFVYSSE
                     SFITTSIMLGATKVGEVWAMLCISLLLVAYLMLKRHKIEALFFALTMALSGILNPALK
                     NIFDRERPTLLRLIDITGFSFPSGHAMGSTAYFGSGIYLLNRLNQGNSKGILIGLCAA
                     MILLISISRVYLGVHYPTDIIAGIIGGLFCIILSTLLLRNKLIN"
     gene            8421..8621
                     /locus_tag="ACGEXS_001854"
     CDS             8421..8621
                     /locus_tag="ACGEXS_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012854.1"
                     /translation="MTNPTRTKRCGGDVLCGIDNHFQLLLYISENHCQLETKTFFEYF
                     LRIVNKTHDYAILENKKICISH"
     gene            8698..8829
                     /locus_tag="ACGEXS_001855"
     CDS             8698..8829
                     /locus_tag="ACGEXS_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012855.1"
                     /translation="MLLPSSKSHIEMMCEMFKLRSFCENKDNNYSFYNSEKSVYDNN"
BASE COUNT         3354 a         1211 c         1607 g         2814 t
ORIGIN      
        1 ggcttttgaa aattatatca aatcagacga gtataaaggt ccagatatga cagatttcgg
       61 gcatcgtgca ttgagtgatc ctgaatttaa cgaacaatat aaagaacaaa tgaaacaatt
      121 taagcaacgt attcttgaag atgatacatt tgcgaagcaa ttcggggatt taggaaatgt
      181 ttatggtaaa caatggcgag attgggttga taaagatggt aatcattttg atcaacttaa
      241 aacagtaatt gaacaaatta agcataatcc agattcaagg cgacacatcg tatctgcatg
      301 gaatccaaca gaaattgata caatggcact tccgccttgt cataccatgt tccagtttta
      361 tgtccaagat ggtaagttaa gttgccagtt ataccaacgt agcgcagata tctttttagg
      421 tgtgccattt aatatcgcaa gctacgcttt attgacacac cttattgcca aagaatgtgg
      481 acttgaagtg ggtgaatttg tgcatacatt tggagatgca catatttatt caaatcatat
      541 tgatgcgatt caaacacaat tagcacgtga aagcttcaat cctccaacat taaaaattaa
      601 cagtgacaag tctattttcg acataaatta tgaagatttg gaaattgttg actatgaatc
      661 acatccagca ataaaagctc caatagcagt gtagtcattg catagttagc taaccatata
      721 gacatcaaaa tgacatcata gtattttcaa gtgcaaaaaa gtactttttt gtgttaaacg
      781 ttttcataaa ttatgcaaaa tcattatttc tatcacactt tatgataaaa attgtgttaa
      841 attaaagata acttagtaat aaaaaatgaa atgatagaag aaggaggata attatgactt
      901 tatccattct agttgcacat gacttgcaac gagtaattgg ttttgaaaat caattacctt
      961 ggcacctacc aaatgatttg aagcatgtta aaaaattatc aacaggtcat actttagtaa
     1021 tgggtcgtaa gacatttgaa tcgattggta aaccactacc gaatcgtcga aatgttgtac
     1081 ttacttcaga tacaagtttc aacgtagagg gcgttgatgt aattcactct attgaagata
     1141 tttaccaact accgggccat gttttcatat ttggagggca aacattattt gaagaaatga
     1201 ttgataaagt ggacgacatg tatattactg ttattgaagg taaattccgt ggtgatacgt
     1261 tctttccacc ttatacattt gaagactggg aagttgcctc ttcagttgaa ggtaaactag
     1321 atgagaaaaa tacaattcca catacctttc tacatttaat tcgtaaaaaa taagggggaa
     1381 aacgaccatg acaaaacaga ttatagtaac agactcaaca tccgatttat ctaaagaata
     1441 cttagaagca aacaacattc atgtaattcc tttaagttta actattgaag gagcttcata
     1501 cgttgaccaa gtagatatta catcagaaga atttattaat catattgaaa atgatgaaga
     1561 tgtaaagaca agtcagccag ccataggtga atttatatct gcttatgaag aactaggaaa
     1621 agatggctct gaaatcataa gtattcatct ttcttcagga ttaagtggta catataacac
     1681 tgcttaccaa gcaagtcaaa tggtagatgc taatgtaact gttattgatt caaaatctat
     1741 ttcttttggt ttagggtatc aaatacaaca cctagtagag cttgtaaaag aaggtgtctc
     1801 aacttctgaa atagttaaaa agttaaatca tttaagagaa aacattaaat tatttgtagt
     1861 tatagggcaa ttgaatcaat taattaaagg tggcagaatt agtaaaacaa aaggtttgat
     1921 tggtaatctt atgaaaatta aaccaattgg tacactagat gatggtcgct tagagcttgt
     1981 gcacaatgcg agaactcaaa attctagtat ccaatacttg aaaaaggaaa ttgctgaatt
     2041 tataggagat catgaaatca aatccattgg tgtcgcacat gctaacgtca ttgaatatgt
     2101 tgataaattg aagaaagttt ttaatgaagc ttttcatgtg aataattacg atataaatgt
     2161 aactacacca gttatttctg cacatactgg tcaaggtgcg attggcctcg tagtccttaa
     2221 gaagtaaatt taatcttttc agtgttaatt acttccattt caatccttta tagactaaat
     2281 ttataattag atagatagag gaggtaattc atatgacaaa agaatatgca acattagcag
     2341 gaggatgttt ctggtgcatg gttaaaccat ttacatcata tccaggcatc aagtcagtcg
     2401 tatctggtta tagtggcggt catgttgaca acccaactta tgaacaggta tgtacgaatc
     2461 aaaccggcca tgtcgaagca gtacaaatta cgtttgatcc agaggttact tcctttgaaa
     2521 atatattaga catatatttc aaaacatttg acccaactga tgatcaaggg caatttttcg
     2581 atagaggcga aagctatcaa ccagtcattt tctatcatga tgaacatcag aaaaaggctg
     2641 ctgagtttaa aaagcaacaa ttaaatgaac aaggtatttt caagaaacca gtgattacac
     2701 ctattaaacc atataaaaat ttctatccag ctgaagacta ccatcaagat tattacaaaa
     2761 agaacccggt acattattac caatatcaac gtggttcagg tagaaaagcg tttatagaat
     2821 cacattgggg gaatcaaaat gcttaaaaaa gataaaagtg aactaacaga tatagaatat
     2881 attgttacac aagaaaacgg cactgaacca ccatttatga atgaatattg gaatcatttt
     2941 gctaaaggaa tttatgtaga taaaatttct ggtaaacctt tatttacatc tgaagaaaag
     3001 tttcattctg aatgtggatg gcctagcttt tccaaagcgc ttgatgacga tgaaattata
     3061 gaattagtcg acaaatcatt tggtatgttg agaactgaag tgcgttcaga agaatcaaat
     3121 agtcatttag gacatgtctt taatgatgga cctaaagaaa gtggcggctt aagatactgt
     3181 atcaattccg ctgcaattca atttattcca tatgaaaagt tagaagaatt gggttatggc
     3241 gatttaatat cacattttga taagtaggag tggaaagtat gtttaaaaaa ttattcggaa
     3301 aaggcaaaga agttcagaaa gatatcgcaa tttatgcacc attaactgga gaattcgtga
     3361 aaattgaaga tattccagat cctgtattcg cacaaaaaat gatgggcgaa ggttttggta
     3421 ttaatccaac tgaaggagaa gttgtgtctc caattgcagg acgtgttgac aatgtctttc
     3481 caactaagca tgcgattggg ttaaaagcag ataacggatt agaattatta gttcatatcg
     3541 gtttagacac agttcaatta gacggtgaag gatttgaagt gttagtgtct agtggtgacg
     3601 aagttaacgt cggagatcca ttagtaagat tcaaccttga atatattaat aataacgcta
     3661 aatctgtaat ttcaccaatt ataattacta acactgatca agcagcttca attaatattt
     3721 atgatgaaaa tgctgtgatt aaaggtgaaa caaaagtgat tgatgtgaca atgaactaat
     3781 gaaaaactat tcgttttatc aatttgtcat gacagttcgt ggtcgacacg acgataaagg
     3841 tcgtctagca gaagagatat ttgacgatct tgctttccca aaacacgatg atgattttaa
     3901 catactgtct gattatattg agacacatgg tgatttcaca ttgccaatgt ctgtatttga
     3961 tgatttatat gaagaatata cggaatggct aaaattttaa tataattttt aataataata
     4021 gttagaacca gggtgatgca attcgttatc ctggttttaa tttaaaataa actaagttgt
     4081 gactaaaaat taatcaatta tagtgaaata tggtgcgcta tcttgcataa attgatatga
     4141 ttaactacac agaatttaaa agtacataat acataataag gaagtgatac aatggatgat
     4201 aagcaacaca catcttcatc cgatgatgaa cgcgctgaaa ttgcaacaag caatcaagac
     4261 caagaaacta attcatcgaa acgcgttcac ttaaaacgtt ggcaattcat atcaatatta
     4321 attggtacaa tcctaataac agctgtcatc acagttgttg catatatttt tataaatcaa
     4381 aaaataagtg gtttaaacaa aactgatcaa gcaaacttaa ataaaattga aaatgtgtat
     4441 aaaatcttaa atagtgatta ttacaaaaaa caggactctg acaagttaag taaagctgca
     4501 attgatggca tggtcaaaga attaaaagat ccttattctg aatatttaac aaaagaacaa
     4561 acgaaatcct ttaatgaagg tgtttcaggt gattttgtag gtattggtgc agaaatgcaa
     4621 aagaaaaatg atcaaattat ggttactagt cctatgaagg gatctccagc agaacgtgct
     4681 ggcattcgtc ctaaagatgt cattactaaa gtaaatggaa aatcaattaa aggtaaagca
     4741 ttagatgaag ttgtcaaaga tgttcgtggt aaagaaaaca ctgaagtcac tttaactgtt
     4801 caacgaggta gtgaagaaaa agacgttaag attaaacgtg aaaaaattca tgttaaaagt
     4861 gttgagtata agaaaaaagg taaagttgga gttattacta ttaataaatt ccagaatgat
     4921 acatcaggtg aattgaaaga tgcagttcta aaagctcaca aagatggttt gaaaaagatt
     4981 gttttagatt taagaaataa tccaggtgga ctactagatg aagctgttaa aatggcaaat
     5041 atttttatcg ataaaggaaa aactgttgtt aaactagaaa aaggtaaaga tactgaagca
     5101 attcaaactt ctaatgatgc gttaaaagaa gcgaaagaca tggatatatc catcttagtg
     5161 aatgaaggtt cagctagcgc ttctgaagtg tttactggtg cgctaaaaga ctataataaa
     5221 gctaaagttt atgggtcaaa aacattcggc aaaggtgtcg tacaaactac aagagagttt
     5281 aaggatggtt cattgttaaa atatactgaa atgaaatggt taacgccaga tggtcattat
     5341 attcacggta aaggcattaa accagacgtt actattgaca cacctaaata tcaatcttta
     5401 aatgtcattc ctaatacgaa aacatttaaa gttggagacg atgataaaaa tattaaaact
     5461 attaaaattg gtttatcagc tttaggttat aaagttgata atgaatcaac gcaatttgat
     5521 aaagctttag aaaatcaagt taaagctttc caacaagcga ataaacttga ggtaactggt
     5581 gaatttaata aagaaacgaa taataaattt actgagttat tagttgaaaa agctaataaa
     5641 catgatgatg ttctcgataa gttgattaat attttaaaat aagcgataca cactactaaa
     5701 attgtattat tattatgtta atgacacgcc tcctaaattt gcaaagatag caatttagga
     5761 ggcgtgttta tttttattga cgtctaactc taaaagatat aaattagaca tttacaaatg
     5821 atgtaaataa cgcaatttct atcatcgctg ataacaattc atggtttaat atgcaatgag
     5881 catatacttt ttaaatagta ttattcacta gttttaacaa tcaattaatt ggtatatgat
     5941 acttttattg gttattttta tcccatagtg tgataattac tatttttcat tcataataaa
     6001 ggtttaaagc atgttaatag tgtgtaagat taacatgtac tgaaaaacat gtttaacata
     6061 atgatataag gagtgacgta catgatccgt ctaggtaaaa tgtcagattt agatcaaatc
     6121 ttaaatctag tagaagaagc aaaagaatta atgaaagaac acgacaacga gcaatgggac
     6181 gatcagtacc cacttttaga acattttgaa gaagatattg ctaaagatta tttgtacgta
     6241 ttagaggaaa atgacaaaat ttatggcttt attgttgtcg accaagacca agcagaatgg
     6301 tatgatgaca ttgactggcc agtaaataga gaaggcgcct ttgttattca tcgattaact
     6361 ggttcgaaag aatataaagg agctgctaca gaattattca attatgttat tgatgtagtt
     6421 aaagcacgtg gtgcagaagt tattttaacg gacacctttg cgttaaacaa acctgcacaa
     6481 ggtttatttg ccaaatttgg atttcataag gtcggtgaac aattaatgga atatccgcca
     6541 tatgataaag gtgaaccatt ttatgcatat tataaaaatt taaaagaata gaggtaatat
     6601 taatgacgaa aatcgcattt accggagggg gaacagttgg acacgtatca gtaaatttaa
     6661 gtttaattcc aactgcatta tcacaaggtt atgaagcgct ttatattggt tctaaaaatg
     6721 gtattgaaag agaaatgatt gaatcacaac taccagaaat taagtattat cctatttcga
     6781 gtggtaaatt aagaagatat atttctttag aaaatgccaa agacgtattt aaagtattga
     6841 aaggtattct tgatgctcgt aaagttttga aaaaagaaaa acctgatcta ttattttcaa
     6901 aaggtggatt tgtatctgtg cctgttgtta ttgcagccaa atcattaaat ataccaacta
     6961 ttattcatga atctgactta acaccaggat tagcgaataa gatagcactt aaatttgcca
     7021 agaaaatata tacaacattt gaagaaacgc taaactactt acctaaagag aaagctgatt
     7081 ttattggagc aacaattcga gaagatttaa aaaatggtaa tgcacataat ggttatcaat
     7141 taacaggctt taatgaaaat aaaaaagttt tactcgttat gggtggaagc ttaggaagta
     7201 aaaaattaaa tagcattatt cgcgaaaact tagatgcatt attacaacaa tatcaagtga
     7261 tacatttaac tggtaaagga ttaaaagatg ctcaagttaa aaaatcagga tatatacaat
     7321 atgaatttgt taaagaggat ttaacagatt tattagcaat tacggataca gtaataagta
     7381 gagctggatc aaatgcgatt tatgagttct taacattacg tataccaatg ttattagtac
     7441 cattaggttt agatcaatcc cgaggcgacc aaattgacaa tgcaaatcat tttgctgata
     7501 aaggatatgc taaagcgatt gatgaagaac aattaacagc acaaatttta ttacaagaac
     7561 taaatgaaat ggaacaggaa agaactcgaa ttatcaataa tatgaaatcg tatgaacaaa
     7621 gttatacgaa agaagcttta tttgataaga tgattaaaga cgcattgaat taatgggggg
     7681 taatgcttta tgagtcaatg gaaacgtatc tctttgctca tcgtttttac attggttttt
     7741 ggaattatcg cgtttttcca cgaatcaaga cttgggaaat ggattgataa tgaagtttat
     7801 gagtttgtat attcatcaga gagctttatt acgacatcta tcatgcttgg ggctactaaa
     7861 gtaggtgaag tctgggcaat gttatgtatt tcattacttc ttgtggcata tctcatgtta
     7921 aagcgccaca aaattgaagc attatttttt gcattaacaa tggcattatc tggaattttg
     7981 aatccagcat taaaaaatat attcgataga gaaagaccta cattgctgcg tttaattgat
     8041 ataacaggat ttagttttcc tagcggtcat gctatgggat caactgcata ttttggaagt
     8101 ggtatctatc tattaaatcg attaaatcaa ggtaattcaa aaggtattct tatagggtta
     8161 tgtgcagcta tgattttatt gatttccata tcacgtgtat atctaggtgt acattatcca
     8221 acagatatta ttgccggcat tattggtgga ttattttgca ttattttatc aacgttatta
     8281 cttagaaata aattaataaa ttaaatagta aaaaaacaaa agcagtaaac ctaaagtgtc
     8341 gtaagggttt actgctttta taaaacgttg ttataacgta tattgtcttt tacgggcata
     8401 taaaagggga atatttgaaa atgaccaatc caacaagaac gaaacgttgt gggggggatg
     8461 ttctatgtgg tattgataat cattttcaac tactattata cattagtgag aatcattgtc
     8521 aattagaaac taaaactttt tttgaatatt ttttaagaat agtaaataaa acgcatgatt
     8581 acgctatttt agaaaataaa aaaatttgta tttctcatta gaattagaat atttaaaagt
     8641 gatgaggttt aaacattata ttgtttacat actccttttg aattcataca ttatgaaatg
     8701 ttacttccaa gttcaaaatc gcacattgaa atgatgtgtg aaatgtttaa actacggtca
     8761 ttttgtgaaa ataaagataa taactattca ttttacaata gtgaaaagtc agtatatgac
     8821 aacaattaat attgcggtaa ggccttgtgt tacagtattc tatatttaag tactgcaatc
     8881 agaattaaca gaatgccatt aactgattat taaatatttg agttaataaa taattaatga
     8941 ttgtagcttg aaaaatttaa aacatggtta ttgatttgtg ataaaa
//
LOCUS       DAXWFK010000104         8759 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.104, whole genome shotgun sequence.
ACCESSION   DAXWFK010000104 DAXWFK010000000
VERSION     DAXWFK010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8759
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.104"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(60..221)
                     /locus_tag="ACGEXS_001856"
     CDS             complement(60..221)
                     /locus_tag="ACGEXS_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012856.1"
                     /translation="MLLILELRFWNLEFGFRNSTFGIQNYNEWKILIIACLMTWFCSD
                     FYLMNCFFV"
     gene            complement(258..998)
                     /locus_tag="ACGEXS_001857"
     CDS             complement(258..998)
                     /locus_tag="ACGEXS_001857"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFF8012857.1"
                     /translation="MFNLKHDLETLKHIIDSSNRITFFTGAGVSVASGVPDFRSMGGL
                     FDEISKDGLSPEYLLSRDYLEDDPEGFINFCHKRLLFVDTMPNIVHDWIAKLERNQQS
                     LGVITQNIDGLHSDAGSQHVDELHGTLNRFYCNVCHKSYTKSDVIDRTLKHCDNCGGA
                     IRPDIVLYGEMLDQPTIIRALNKIEHADTLVVLGSSLVVQPAAGLISHFKGDNLIIIN
                     KDRTPYDSDATLVIHDDMVSVVKSLMTE"
     gene            1240..1995
                     /locus_tag="ACGEXS_001858"
     CDS             1240..1995
                     /locus_tag="ACGEXS_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFF8012858.1"
                     /translation="MNKHERLDEIAKLVNKKGTIRTNEIVEGLNVSDMTVRRDLIELE
                     NKGILTKIHGGARSNSTFQYKEISHKEKHTRQIAEKRFIAKKAASLIEDGDTLFFGPG
                     TTVELLAEEVNHHTLTIITNCLPVYKILLEKQTAHFRVYLIGGEMRHITEAFVGEMAN
                     AMLEKLRFSKMFFSSNAVNKGAVMTSTLDEAYTQQLALSNSIEKYLLIDHTKVGKEDF
                     TSFCQLNELTAVVMDYEDEEKVETIKTYIEVVD"
     gene            2363..2791
                     /gene="lacA"
                     /locus_tag="ACGEXS_001859"
     CDS             2363..2791
                     /gene="lacA"
                     /locus_tag="ACGEXS_001859"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HFF8012859.1"
                     /translation="MAIIIGSDEAGKRLKEVIKSYLLDNKYDVVDVTEGQEVDFVDAT
                     LAVAKDVQSQEGNLGIVIDAFGAGSFMVATKIKGMIAAEVSDERSGYMTRGHNNSRMI
                     TMGSEIVGDTLAKNVVKGFVEGKYDGGRHQIRVDMLNKMC"
     gene            2807..3322
                     /gene="lacB"
                     /locus_tag="ACGEXS_001860"
     CDS             2807..3322
                     /gene="lacB"
                     /locus_tag="ACGEXS_001860"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HFF8012860.1"
                     /translation="MKIALGCDHIVTDTKMRVFEFLKSKGHEVIDVGTYDFTRTHYPI
                     FGKKVGEQVVSGNADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSALYAKEELNAN
                     VIGFGGRIIGELLMCDIIDAFINAEYKPTEENKKLIAKIKHLETSNADQADPHFFDEF
                     LEKWDRGEYHD"
     gene            3335..4267
                     /gene="lacC"
                     /locus_tag="ACGEXS_001861"
     CDS             3335..4267
                     /gene="lacC"
                     /locus_tag="ACGEXS_001861"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009024 - tagatose-6-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HFF8012861.1"
                     /translation="MILTLTLNPSVDISYPLTALKLDDVNRVQEVSKTAGGKGLNVTR
                     VLAQVGEPVLASGFIGGELGQFIAKKLDHADIKHAFYNIKGETRNCIAILHEGQQTEI
                     LEQGPEIDNQEAAGFIKHFEQLLEKVEAVAISGSLPKGLNQDYYAQIIERCQNKGVPV
                     ILDCSGATLQTVLENPYKPTVIKPNISELYQLLNQPLDESLESLKQAVSQPLFEGIEW
                     IIVSLGAQGAFAKHNHTFYRVNIPTISVLNPVGSGDSTVAGITSAILNHENDHDLLKK
                     ANTLGMLNAQEAQTGYVNLNNYDDLFNQIEVLEV"
     gene            4271..5251
                     /gene="lacD"
                     /locus_tag="ACGEXS_001862"
     CDS             4271..5251
                     /gene="lacD"
                     /locus_tag="ACGEXS_001862"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFF8012862.1"
                     /translation="MSKSNQKIASIEQLSNNEGIISALAFDQRGALKRMMAKHQTEEP
                     TVAQIEQLKVLVAEELTQYASSILLDPEYGLPASDARNKDCGLLLAYEKTGYDVNAKG
                     RLPDCLVEWSAKRLKEQGANAVKFLLYYDVDDAEEINIQKKAYIERIGSECVAEDIPF
                     FLEVLTYDDNIPDNGSVEFAKVKPRKVNEAMKLFSEPRFNVDVLKVEVPVNMKYVEGF
                     AEGEVVYTKEEAAQHFKDQDAATHLPYIYLSAGVSAELFQETLKFAHEAGAKFNGVLC
                     GRATWSGAVQVYIEQGEDAAREWLRTTGFKNIDDLNKVLKDTATSWKQRK"
     gene            5273..5584
                     /locus_tag="ACGEXS_001863"
     CDS             5273..5584
                     /locus_tag="ACGEXS_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFF8012863.1"
                     /translation="MNREEVQLLGFEIVAFAGDARSKFLEALTAAQAGDFAKADALIE
                     EGNNCIAEAHRAQTSLLAKEAQGDDIAYSVTMMHGQDHLMTTILLKDLMKHLLEFYKR
                     G"
     gene            5590..7302
                     /locus_tag="ACGEXS_001864"
     CDS             5590..7302
                     /locus_tag="ACGEXS_001864"
                     /EC_number="2.7.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0019197 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase complex [Evidence IEA];
                     GO_function: GO:0015155 - lactose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactose-specific PTS transporter subunit EIIC"
                     /protein_id="HFF8012864.1"
                     /translation="MMQKLIAQIEKGKPFFEKLSRNIYLRAIRDGFISAMPVILFSSI
                     FLLIAYVPNIFGFKWDKGMEAILMKPYNYTMGLVAFLVAGTTAKSLTDSFNRKLESTN
                     QINFISTMLAAMCGFLFLASDPAKDGGFLSAFMGTKGLLTAFLSAFVTVIVYNFCVKR
                     NITIKMPKEVPPNISQVFKDLIPFSAVIIILYALDLVIRNSFKSNVAEGILKLFEPLF
                     TAADGWIGVTIIFGAFALFWFVGIHGPSIVEPAIAAITYANIEANFKLLQAGEHADKI
                     ITSGTQMFIVTFGGTGATLVVPFMFMWMTKSKRNKAIGRASVVPTFFGVNEPILFGAP
                     LVLNPVFFIPFVLAPIVNVWIFKLFVEVLGMNSFSVNLPWTTPGPLGIIMGTGFGLWS
                     FVLAITLIVVDIIIYYPFLKVYDSEILDEEEGRKESNSDLKEKVAANFDTKKADSILA
                     ASGVSDDAAKASNITEQTNVLVLCAGGGTSGLLANALNKAAEEYHVPVKAAAGGYGAH
                     MDIMKEYQLIILAPQVASNYEDIKQDTDRLGIKLAKTQGAEYIKLTRDGQAALDFVQQ
                     QFEN"
     gene            7320..8732
                     /gene="lacG"
                     /locus_tag="ACGEXS_001865"
     CDS             7320..8732
                     /gene="lacG"
                     /locus_tag="ACGEXS_001865"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFF8012865.1"
                     /translation="MTKTLPEDFIFGGATAAYQAEGATNTDGKGRVAWDTYLEENYWY
                     TAEPASDFYNRYPVDLELSEKFGVNGIRISIAWSRIFPNGYGEVNPKGVEYYHKLFAE
                     CHKRHVEPFVTLHHFDTPEVLHKDGDFLNRKTIDYFVDYAEYCFKEFPEVKYWTTFNE
                     IGPIGDGQYLVGKFPPGIKYDFEKVFQSHHNMMVAHARAVKLFKDGGYKGEIGVVHAL
                     PTKYPFDPSNPEDVRAAELEDIIHNKFILDATYLGKYSRETMEGVQHILSVNGGKLNI
                     TDEDYAILDAAKDLNDFLGINYYMSDWMRGYDGESEITHNATGDKGGSKYQLKGVGQR
                     EFDVDVPRTDWDWMIYPQGLYDQIMRVVKDYPNYHKIYITENGLGYKDEFIESEKTVH
                     DDARIDYVRQHLNVIADAIIDGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQE
                     RYPKKSAYWYKELAETKEIK"
BASE COUNT         3094 a         1329 c         1675 g         2661 t
ORIGIN      
        1 tattttatgt ttatttttaa tcttacaaat gacaaaccat taaaagtagc atcccaacat
       61 caaacaaaaa aacaattcat caaataaaaa tcgctacaaa accaagtcat taaacacgca
      121 ataattaaaa ttttccactc attataattc tgaattccaa atgtcgaatt ccgaaaacca
      181 aactccaaat tccaaaaacg caactccaaa attaaaagca tttccctacc attcgggaaa
      241 tgctttttac atactgatta ctctgtcatt aatgatttta caacggaaac catgtcgtca
      301 tgtatgacca aagtagcgtc gctatcataa ggtgttcgat ctttattgat aataattaaa
      361 ttgtcgcctt taaaatgtga tattaatcct gcggcaggtt gtacaacgag tgatgaacct
      421 agtacaacaa gggtgtcagc atgttcaatt ttatttaatg cccttatgat ggtaggttga
      481 tctaacattt caccgtataa tacgatgtcc ggtcgaatgg caccaccaca attatcacaa
      541 tgtttcaaag ttctatcaat aacatctgac ttcgtataag atttatgaca tacattacaa
      601 taaaaacgat ttaacgtgcc atgtaattca tcaacatgtt gacttccagc gtctgagtgc
      661 aaaccatcga tattttgcgt gatgacacct aaagattgtt gattacgttc taattttgca
      721 atccaatcat gaacgatatt gggcatcgta tcgacaaata gtaagcgctt atggcagaaa
      781 ttgataaaac cttcaggatc atcttctaaa taatcacggc ttaacaagta ttctggcgaa
      841 agcccatctt ttgaaatttc atcaaataag ccacccattg aacggaaatc tggaacgcca
      901 cttgcgacag atacaccagc acctgtaaaa aatgtaatac gattcgaact atctataata
      961 tgttttagtg tctctaaatc atgtttcaag ttaaacaccc catcaaaata atgaatgatt
     1021 tataacgtct tttactttta ttatagtata ttcattttac gattatcaaa agcgcagttt
     1081 aaacagttct ttttaaaaat gaattataca tataatcgaa cagttaaatt atattagttt
     1141 ttacgcaatt tttattatag aataagcatg tatattaaaa ttgatgtcta aacataaagg
     1201 ataatgaccc atcgcctaaa taaaggaggc aattttacat tgaataaaca tgaacgtttg
     1261 gacgaaattg ctaaactagt gaataaaaag ggcacgataa gaacgaatga aatcgtcgaa
     1321 ggtttaaatg tgtctgatat gacagttcga agagatttaa ttgaattgga aaataaaggg
     1381 attttaacga agattcatgg tggtgcacgc agtaattcaa catttcagta taaagaaata
     1441 tcgcataaag aaaaacatac ccgtcaaatt gcagaaaaac gatttatagc taaaaaagct
     1501 gcatcattaa ttgaagatgg ggatactttg tttttcggac caggaacaac agtggaacta
     1561 ttagcagaag aagtcaatca tcatacgctc acaattatta cgaattgttt gccggtgtat
     1621 aaaattttgt tagaaaaaca aacagcacat tttcgtgtct atttaattgg tggtgaaatg
     1681 cgccatatta cagaagcatt tgtaggtgaa atggcgaacg ccatgttgga aaaactaaga
     1741 tttagcaaga tgttctttag tagtaacgca gtaaataaag gtgctgtgat gacatctaca
     1801 ttagatgaag cctatacgca acaactggca ctaagtaatt caattgaaaa atacttgtta
     1861 atcgatcata cgaaagttgg caaagaagat tttacatcat tttgtcagtt aaacgaattg
     1921 actgcagtgg tcatggacta tgaagatgaa gaaaaagtag aaacgattaa aacatatatt
     1981 gaagtagtcg actaaattaa aagtataact gcattgttaa aatatgtatc tatatgctca
     2041 taagtgaatg tgaatttgca ttcatttatg ggctattttt tatgcaaatt tatgtcaacg
     2101 ggaacctcga caaagtttta ctacaatttc agacgaatac ctaattaaaa caacaccaca
     2161 atatgaatgt tcacttattg tttgaaaata cgtcataaac aaacatatta taaacatatt
     2221 atgttgattt attgtttgtt tatgtttata atttaaacgt aatcaaattg aaagcctttt
     2281 ctcaagattt tcataaccta caaataaata tgtcacattt taagaaaggt atttcaaaat
     2341 taaagtaaaa aggagtctta ttatggcgat tattattggt tcagatgaag ctggcaaacg
     2401 attaaaagaa gtcatcaaat catacttatt agacaataaa tatgatgttg ttgacgtaac
     2461 agaaggacag gaagttgact ttgttgatgc aactttggct gtagcaaaag atgttcaaag
     2521 tcaagaaggt aacttaggta ttgttatcga tgcatttggt gcaggtagct ttatggttgc
     2581 tacaaaaatc aaaggcatga ttgctgctga agtatcagat gaacgctctg gatatatgac
     2641 tagaggccat aacaattcaa gaatgatcac tatgggatct gaaattgttg gagacacatt
     2701 agcgaaaaat gtcgttaaag gttttgttga aggtaaatac gatggtggta gacaccaaat
     2761 ccgcgtagat atgcttaata aaatgtgcta aggaggaaaa attactatga agattgcatt
     2821 aggatgcgac catattgtta cagatacaaa aatgcgtgta tttgaatttt taaaatcaaa
     2881 aggacatgaa gtcattgacg taggaacata cgatttcaca agaacacatt atccaatttt
     2941 tggtaaaaaa gttggcgaac aagttgttag cggtaatgca gacttaggtg tttgtatttg
     3001 tggaacaggt gttggtatta acaatgctgt aaataaagta cctggcgttc gttcagcact
     3061 agtacgtgat atgacatcag cgttatacgc taaagaagaa ttaaatgcga acgttattgg
     3121 cttcggtgga cgtattatag gtgagttatt aatgtgcgat attatcgatg catttattaa
     3181 tgctgaatat aaaccaactg aagagaacaa aaaattaatc gctaaaatta aacatttaga
     3241 aacaagcaat gcagatcaag ctgatccaca tttctttgat gaattcttag aaaaatggga
     3301 cagaggcgaa taccacgatt aagggacgac acatatgatt ttaactttaa cattaaatcc
     3361 atccgttgat atttcatacc cattaacagc attgaaatta gatgatgtta atcgtgttca
     3421 agaagtaagt aaaacagctg gtggtaaagg tttaaatgta acaagagtac ttgcgcaagt
     3481 aggtgagcca gtccttgcga gtggttttat tggcggtgaa ttaggtcaat ttattgctaa
     3541 aaaattagat catgccgaca tcaagcatgc attttataac attaagggtg aaacacgaaa
     3601 ctgcattgca attttacatg aagggcaaca aacagaaatt ttagagcagg gaccggagat
     3661 tgataatcaa gaggctgcag gatttattaa acattttgaa caattattag aaaaagttga
     3721 agcagttgct atttcaggta gcttacctaa aggattaaat caagattatt atgcacaaat
     3781 cattgaacga tgccaaaaca aaggtgtgcc tgtgatatta gattgctcgg gtgcaacttt
     3841 gcaaacagtt ttggaaaatc catacaaacc tacagtcatt aaaccaaaca tttctgaact
     3901 atatcaattg cttaaccagc cacttgatga aagtttagaa agtttgaaac aggcagtgtc
     3961 acaaccctta ttcgaaggga ttgagtggat tattgtgtct ttaggtgcac aaggtgcatt
     4021 cgctaagcac aatcatacat tctatagagt gaatattccg acaataagtg tattaaatcc
     4081 ggttggttca ggtgattcta cagttgctgg aattacttca gcaattctta atcatgaaaa
     4141 cgaccacgat ttactaaaaa aagccaatac tttaggcatg ctgaatgcac aagaagcaca
     4201 aacaggttat gtcaatttaa ataattatga tgatttattc aatcaaattg aagttttaga
     4261 ggtgtaaaaa atgtcaaaat cgaatcaaaa aatcgcatca attgaacaat taagtaacaa
     4321 cgaaggtatc atttctgcat tagcatttga ccaacgtggt gctttaaaac gcatgatggc
     4381 aaaacatcaa actgaagaac caactgtagc tcaaattgaa caattaaaag tattagttgc
     4441 ggaagaatta acacaatatg catcttcaat tttattagat cctgaatatg gtttaccagc
     4501 ttcagatgca cgtaacaaag attgtggatt attacttgct tatgaaaaaa ctggatatga
     4561 cgtaaacgct aaaggtcgtt tacctgactg tttagtagag tggtctgcaa aacgtttaaa
     4621 agaacaaggc gcaaatgctg ttaaattctt actttactat gatgttgatg atgctgaaga
     4681 aattaacatt caaaagaaag catacattga aagaatcggt tcagaatgtg ttgcagaaga
     4741 tattccattc tttttagaag tattaacata cgatgacaac attcctgata acggtagtgt
     4801 tgaattcgca aaagttaagc ctagaaaggt taacgaagca atgaaattat tctcagaacc
     4861 acgctttaac gttgatgtat tgaaagttga agtaccagta aacatgaaat atgtagaagg
     4921 ctttgctgaa ggtgaagtgg tttatacaaa agaggaagcg gcacaacact ttaaagatca
     4981 agatgcagca actcatttac catacattta cttaagtgct ggtgtttctg cggaattatt
     5041 ccaagaaact ttaaaatttg ctcacgaagc tggtgctaaa ttcaacgggg tactttgtgg
     5101 ccgtgctaca tggtctggtg ctgttcaagt atacattgaa caaggtgaag atgctgctcg
     5161 tgaatggtta cgtacaactg gtttcaaaaa tattgatgac ttaaataaag tattaaaaga
     5221 tactgcgaca tcttggaaac aaagaaaata agtttaaaag gggattaatg ctatgaatag
     5281 agaagaagtc caattattag gttttgaaat tgttgcattt gcaggggatg cacgttctaa
     5341 gtttttagaa gcattaacag cagctcaagc tggagatttt gcaaaagcag atgcactgat
     5401 tgaagaagga aacaattgca ttgcggaagc gcatagagca caaacaagcc tgttggctaa
     5461 agaagcgcaa ggtgatgata ttgcatacag tgtaacgatg atgcatggtc aagatcattt
     5521 aatgacaaca attttactga aagatttaat gaagcattta ttagaatttt ataaaagagg
     5581 gtgatgacaa tgatgcaaaa attgatcgca caaattgaaa aagggaagcc atttttcgag
     5641 aaattatcac gtaacatata tttaagagcc atccgtgatg gatttatttc tgcaatgcct
     5701 gtcatcttat tctcaagtat ctttctatta attgcatatg tgccaaatat atttggtttt
     5761 aaatgggata aaggcatgga agccatttta atgaaaccat ataactatac gatggggctt
     5821 gtagcatttt tagtagcagg aacgacagcg aaatctttaa cagattcatt taaccgtaag
     5881 ctagaaagta caaatcaaat taactttatt tctacaatgc tagcagcgat gtgtggattc
     5941 ttgttcttag cttctgatcc agccaaagat ggtggattct taagtgcatt tatgggaact
     6001 aaaggtttat taacagcatt cttatcagca tttgtaactg ttattgttta taacttctgt
     6061 gtgaagcgca atattacaat taaaatgccg aaagaagtac cgccgaatat ttcacaagta
     6121 tttaaggact taattccatt ttcagcggta atcatcattc tttatgcatt agatttagtc
     6181 attcgcaaca gctttaaatc aaatgtagcg gaaggtattt taaaattatt cgaaccatta
     6241 tttacagcag cagatggatg gattggtgtc acaattatct ttggtgcctt tgcattattc
     6301 tggtttgtag gtattcatgg tccgtcaatt gtagagccag caattgcagc cattacatat
     6361 gcgaatatcg aagcgaactt caagttgctt caagctggag aacacgcaga taaaattatt
     6421 acatcaggta cacaaatgtt tatcgttaca tttggaggta cgggtgcaac attggtcgtc
     6481 ccgttcatgt ttatgtggat gacgaaatct aaacgaaaca aagcgattgg acgtgcatca
     6541 gttgtaccaa cattctttgg tgtaaacgaa ccaattttat ttggtgcacc actagtattg
     6601 aatcctgtgt tctttattcc atttgtatta gcaccaattg ttaacgtatg gattttcaaa
     6661 cttttcgttg aagtgttagg aatgaatagt tttagtgtga atttaccttg gacaacacca
     6721 ggtccattgg gcattatcat gggtacaggt tttggtttat ggtcattcgt actagctatt
     6781 actttgattg ttgtagatat tattatttac tacccattcc taaaagttta tgatagtgaa
     6841 attcttgatg aagaagaagg acgtaaagaa agtaattcag atttaaaaga aaaagttgca
     6901 gcaaactttg atacgaaaaa agctgattca attttagcgg caagtggtgt atcagacgat
     6961 gcagctaaag cttcaaatat cacagaacaa acaaatgtct tagtactttg tgccggtggt
     7021 ggtacaagtg gattattagc caatgcactt aataaagcag ctgaagaata tcatgtgcca
     7081 gtgaaagcgg cagctggcgg atatggcgca catatggaca ttatgaaaga gtaccaatta
     7141 atcattttag cgccacaagt tgcgtctaac tatgaagata ttaagcaaga tacagataga
     7201 ttaggtatta aattagcgaa gactcaaggt gctgaatata tcaaattgac acgagatggc
     7261 caagcagccc ttgatttcgt tcaacaacaa tttgaaaatt aagaatagga gtttttcata
     7321 tgactaaaac attacctgaa gattttattt ttggtggagc aacagcagca tatcaagcag
     7381 aaggtgcgac aaatacggat ggtaaaggtc gcgtagcatg ggatacgtat ttagaagaaa
     7441 actattggta cactgcagaa ccagcgagtg atttttataa cagatatcca gtggatttag
     7501 aattaagtga aaaattcggt gttaatggta tccgaatttc aattgcatgg tctcgtatct
     7561 tcccaaatgg atatggcgaa gttaacccta aaggtgttga gtattaccat aagttatttg
     7621 cagagtgtca taaacgtcat gttgaaccat ttgtaacatt gcatcatttt gatacaccag
     7681 aagtgttaca caaagatggc gattttctaa atcgtaaaac gattgactac tttgtagatt
     7741 atgctgaata ttgttttaaa gaattcccag aagtaaaata ttggactaca ttcaatgaaa
     7801 ttggacctat tggtgatggt caatatttag taggtaaatt ccctccaggt attaaatatg
     7861 attttgaaaa agtattccaa tcacatcata acatgatggt ggctcatgct agagcagtaa
     7921 aattatttaa agatggcgga tacaaaggag aaattggtgt tgtacacgca ttgccaacaa
     7981 aatatccgtt cgatccatca aatcctgaag atgttcgtgc agcagaatta gaagatatca
     8041 tccataataa attcatctta gatgcaacat atttaggtaa gtattctcgt gaaacgatgg
     8101 aaggtgtgca acatatctta tctgttaatg gcggtaaatt aaacattaca gatgaagatt
     8161 atgccatttt agatgcagcc aaagatttaa acgacttctt aggtatcaat tactacatga
     8221 gtgattggat gagaggttac gatggagaat ctgaaatcac gcataatgcg acaggtgata
     8281 aaggcggttc taaataccaa cttaaaggtg tcggacaacg tgaatttgat gtcgacgtac
     8341 ctcgcacaga ctgggactgg atgatctatc ctcaaggttt atatgatcaa atcatgcgtg
     8401 ttgttaaaga ttatcctaac tatcataaga tttatatcac tgaaaatggt ttaggatata
     8461 aagatgaatt tattgaatct gaaaaaacag ttcatgatga tgcacgtatt gattatgtaa
     8521 gacaacattt gaacgttatt gcagatgcta ttatagatgg tgcaaatgtt aaaggttact
     8581 ttatttggtc attaatggat gtgttctctt ggtcaaatgg ttatgaaaaa cgatatggtt
     8641 tattctatgt cgattttgag actcaagaac gttatcctaa gaaaagtgct tattggtaca
     8701 aagagttagc cgaaacaaaa gaaattaaat aattatagat attaaaaggc ctatcagtc
//
LOCUS       DAXWFK010000105         8718 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.105, whole genome shotgun sequence.
ACCESSION   DAXWFK010000105 DAXWFK010000000
VERSION     DAXWFK010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8718
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.105"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..1522
                     /locus_tag="ACGEXS_001866"
     CDS             <1..1522
                     /locus_tag="ACGEXS_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6583 family protein"
                     /protein_id="HFF8012866.1"
                     /translation="SKKLKIIIPIIIVLLLIGGIAWGVYAFFANTPKNTYLKSEQQTA
                     KMYKDYFNDRFENEVKFQEKMKDNSFLSSLELSADASDEIVKGLGIPKSVVNASKIKM
                     SYGHDPKKEKSMINLEPTIADSALGKFQLAADKDKHYFESPLFKGKYSVNNSDLLSTY
                     SKLTGEDEETAKENGITNQQLNLNTLFNNAQAQQSDYSKIAEKYSELIVDKLDDDNFD
                     KGKKEEIKVNGEKYKVRPVTLTLSRADTKKITLAVLEEAKKDKDLKKLMEEQGATKDF
                     EKDIKKAIDDVKETKKDEFAKIQSKIYTEKHTIVKREITITDKENNKTKIKGTNTLED
                     DKLKLDYALDFDQDKYTYAEAKYTIKGVSSKEKDNKYNDKYEFGKKTEYDESKIKLDN
                     QEKVDGTKRQDKGKITVALDKYSDENEFTFENNIDSDVKNNTQKSTLNIGIKYAEEPI
                     NFILKSSTKLKADIDFDDSGAKDFNSLSSKDREKLEKEIEKNGGKMFESILKKASK"
     gene            1590..2756
                     /locus_tag="ACGEXS_001867"
     CDS             1590..2756
                     /locus_tag="ACGEXS_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8012867.1"
                     /translation="MKTIHLFRIYHSFLLKKWYLIIYLLFILAALLITLTTIQHVTED
                     DNHFNIGVVDKDQSSETKLILNSIGKGSNLGKNVSIKAYDDKQAHTLLKKHKLQGYFV
                     FDKGMTKAFYKQGELPISVYTYDQQSMKSVVLSQLTDSVYQRLMRSMGGILAFQDLAP
                     KASHSDSINVMTDLLITGLNRSGAFNLEPIHLYDTGSYYAITGFLTTVFIFALSLFTV
                     LKMNQDTVLKARLKMFHFSKERLLIIRTLITWFYTMLWSIVGVVWIVFSIPNIFELYN
                     WPTLAIHLSYYVTFLILWLLLIELLTTGLLNSISKVILAIVILVLSGLTIPTIFLQHI
                     ANGVFNIQPFAVVTNQLLEIILNNYILELHPSFYLSFIALLIINLAVLVWRYRQ"
     gene            2753..3673
                     /locus_tag="ACGEXS_001868"
     CDS             2753..3673
                     /locus_tag="ACGEXS_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8012868.1"
                     /translation="MKPYIQLVVFKQWLQYILLVTTIVIALVLIGIGYRVAHDNFKIP
                     ITIQDLDQTTASKSFVNKIKQSDYVTIKKVDEDESYIEDDVTKKEAILSMQIPKGFSQ
                     KLKENRLKETIQLYGRDDFIGGIAVEIVSSSLYEQQIPNIIYEHLEDMKQHQSIDAIN
                     KSYHKHTPESKIKFVSLTKQAQHSISISLIFAVILFVSAVQVVLHYRLNQQAALQRLS
                     QYHLSRFKLYSTYVMTHTILLLLVLLAVSLYLSQPLSLIFYLKSLLLILIYEIGIVFI
                     LFHIQTISHRLFMTFIYALAMGIVYLIIFM"
     gene            3687..4346
                     /locus_tag="ACGEXS_001869"
     CDS             3687..4346
                     /locus_tag="ACGEXS_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8012869.1"
                     /translation="MIEINNLSKRYRNKQIFNHLTMSFDSNRLTVLLGDNGAGKSTLL
                     RMIAGIEKANDGTINYFGEKWNQRQIQNHIGYVPQDIALFEHMTVAENIKFFKSLCKN
                     PINDTTINEYLQQLNFDDTSAKVSTLSGGNKRKINILVGLLGQPRILILDEPTVGIDL
                     KSRHDIHQLLNIMKSKCLIILTTHHLDEVEALADDIKLIGQDPFYQHVLEDKQWTYTY
                     Y"
     gene            complement(4398..5348)
                     /gene="lytM"
                     /locus_tag="ACGEXS_001870"
     CDS             complement(4398..5348)
                     /gene="lytM"
                     /locus_tag="ACGEXS_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-glycine endopeptidase LytM"
                     /protein_id="HFF8012870.1"
                     /translation="MKKLTAAAIATMGFATFTMAHQADAAETTNTQQAHTQMSTQSQD
                     VSYGTYYTIDSNGDYHHTPDGNWNQAMFDNKEYSYTFVDAQGHTHYFYNCYPKNANAN
                     GSGQTYVNPATAGDNNDYTASQSQQHINQYGYQSNVGPDASYYSHSNNNQAYNSHDGN
                     GKVNYPNGTSNQNGGSASKATASGHAKDASWLTSRKQLQPYGQYHGGGAHYGVDYAMP
                     ENSPVYSLTDGTVVQAGWSNYGGGNQVTIKEANSNNYQWYMHNNRLTVSAGDKVKSGD
                     QIAYSGSTGNSTAPHVHFQRMSGGIGNQYAVDPTSYLQSR"
     gene            complement(5674..6666)
                     /locus_tag="ACGEXS_001871"
     CDS             complement(5674..6666)
                     /locus_tag="ACGEXS_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HFF8012871.1"
                     /translation="MCTGFTIQTLNNQVLLGRTMDYDYPLDGSPAVTPRNYRWKSRTG
                     TTGQTQYGFIGTGTDMEGFIYGDGVNEHGVAISTQYFRGYSSYGSTHKADAMNITQNE
                     IVTWILGYTTSIEDMKQQASQIHVVAVYLNDIGEVPPLHYHVSDATGHTVEVSFKEGE
                     VVIKDNPIGVLTNHPDLNWHYSNLRQYINISPYPATANLLEGVTIEPLGNEAGTFGLP
                     GGFTSTERFVRMAFMKANIAQNNDKEMDLMNAFYLLDAVNIPIGIVRPHDADNHYTMY
                     QTVINLTTRTLYIKYYGSNELVALKLTDDLINRKDMTIFKPEKHITIRKLNDNQ"
     gene            6900..8276
                     /locus_tag="ACGEXS_001872"
     CDS             6900..8276
                     /locus_tag="ACGEXS_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8012872.1"
                     /translation="MNNTQSSPRSNIIIAIMLSALTYWLFAQSFINIGPLVGQTYQTS
                     PAVLNLSISLTSFATGIFMVAAGDIADKIGQLRMTYMGLIISMFASLLLIISDITALL
                     IIGRILQGLSAAILLPSTVGVLNNQFKGEHLRRAISYLMISTVGGIGLAGVIGGLIAS
                     NFGWQTNFIISIVIAFIAILLLKGTPEKVSQHSHRHPFDYKGMSIFAVMIGSFTLLLT
                     QGFEQGWFSTFSIICLSIFIITTLIFIIIERRHEVPFIDFSVLRNRPFIGAFLNNFVL
                     NSGLGVTVVFFIYAQTHLGLSAAQSGLVTLPYAIVAVAMIRLGEKATLRFGGKLMLII
                     GPLFPVIGITIISMTQLLASQYVIAVIIGFVICAIGNGLVATPGLTIAIFSMPNEKVG
                     LATGLYKMSGTLGGAFGIALSTTVFSMLQLNYAPSVAATVTFIVSIVLMILGSLSAYM
                     IIPKTVKS"
     gene            complement(8353..8703)
                     /locus_tag="ACGEXS_001873"
                     /pseudo
     CDS             complement(8353..8703)
                     /locus_tag="ACGEXS_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2920 a         1476 c         1411 g         2911 t
ORIGIN      
        1 gtctaaaaag ttaaaaatta taattcctat tattattgtc ttactattaa taggtggaat
       61 cgcatgggga gtttatgcat tttttgctaa tacaccgaaa aatacatacc taaaaagtga
      121 acaacaaaca gcaaaaatgt ataaagatta ttttaatgac cgttttgaaa atgaagtgaa
      181 gttccaagaa aagatgaaag ataattcgtt tttatcttca ttagaattaa gcgcagatgc
      241 atcagatgaa attgttaaag ggcttggcat tcctaaatct gttgttaatg catcgaaaat
      301 taaaatgtca tatggacatg atcctaaaaa agagaaatca atgattaatc ttgaaccaac
      361 aatcgcagac tctgcattag ggaaattcca gttagctgca gataaagata agcattattt
      421 cgaatcacca ttatttaaag ggaaatatag tgttaataat tctgatttat tatcaactta
      481 ttcaaaactt acaggtgaag atgaagaaac agcaaaagaa aatggtatta caaaccaaca
      541 actaaattta aatactcttt ttaataatgc tcaagcacaa caaagtgact acagcaaaat
      601 tgccgaaaaa tattccgaac ttattgtcga caaattagat gacgataatt ttgataaagg
      661 taaaaaagaa gaaattaagg ttaatggtga aaagtacaaa gttagacctg tcacgttaac
      721 acttagcaga gctgacacta aaaaaattac attagctgta ttagaagaag ctaaaaagga
      781 taaagacctt aaaaaattaa tggaagaaca aggtgctaca aaagactttg aaaaagacat
      841 caaaaaagca attgacgatg tcaaagaaac taaaaaggat gaatttgcta aaattcaatc
      901 taaaatttat accgaaaaac atacgattgt aaaacgagaa attactatta cagacaaaga
      961 aaataataaa actaaaatca aaggtactaa tactttagaa gacgataagt taaaactaga
     1021 ttacgcactt gatttcgatc aagataaata cacgtatgct gaagcgaaat atacaattaa
     1081 aggcgtatct tctaaagaaa aagataataa atataatgat aaatacgaat ttggtaaaaa
     1141 gacagaatat gatgaatcaa aaatcaaatt agataaccaa gaaaaagtag atggcacaaa
     1201 acgtcaagat aaaggtaaaa tcactgtcgc attagataaa tatagcgacg aaaatgaatt
     1261 cacttttgaa aataatatag attctgacgt aaaaaataac actcagaaat ctacgttaaa
     1321 tatcggcatc aaatatgctg aagaaccaat taatttcatt ttaaaatcta gcacaaaatt
     1381 gaaagcagat attgattttg atgatagtgg tgcaaaagat ttcaatagtc tatcttcaaa
     1441 agaccgtgaa aaacttgaaa aagaaatcga aaaaaatggc ggcaaaatgt ttgaatcaat
     1501 tttaaaaaag gcatctaaat aactaaaata taaaaataag attggaaggt catcttgcct
     1561 ttcaatctta tcattggagg agtggttaaa tgaaaacaat acatttgttt cgcatctacc
     1621 actctttttt attgaaaaag tggtatttga ttatctattt actatttata ttagcggcac
     1681 tccttattac attaacgaca atccaacatg taacagaaga tgacaatcat tttaatatag
     1741 gtgtcgtaga taaagatcaa tcaagtgaaa cgaaattaat cttaaactct attggtaaag
     1801 ggagtaacct aggaaaaaac gtgagcatta aagcatatga tgataagcaa gcacatactt
     1861 tgttaaaaaa acataaactt caaggctatt ttgtttttga taaaggtatg accaaggcat
     1921 tttataaaca aggcgaacta ccaatttcag tatatacata tgatcaacaa tccatgaaaa
     1981 gtgtcgtgct atctcagcta acagattctg tttaccaacg tcttatgcga tcaatgggtg
     2041 gcatcttagc ttttcaagac ttagcaccga aagcatcaca ttctgacagt atcaatgtta
     2101 tgactgattt gctgattaca ggattaaacc gttcaggtgc atttaactta gaaccgattc
     2161 atttatacga tacgggcagt tattatgcaa ttacaggatt tttaacaacg gtattcatct
     2221 ttgcactatc tttatttaca gttttgaaaa tgaatcaaga tactgtattg aaagcgcgat
     2281 tgaaaatgtt tcatttttct aaagagcgtt tattaatcat tcgtacgttg attacatggt
     2341 tttatactat gttatggagt atcgttggtg tagtttggat tgtgttcagt attccgaata
     2401 tctttgaatt atataattgg ccaacgttag ccattcattt aagctattat gttacctttt
     2461 taatactgtg gttattactc atcgaacttt taacaacagg cttgttgaat agtattagta
     2521 aagtcatatt agctattgtc atcctggtgt tatctggttt aacaatacct acgatttttt
     2581 tacaacatat agcaaatggt gttttcaata ttcaaccgtt cgcagttgta acaaatcaat
     2641 tgttagaaat tattttaaac aactacattt tagaactgca ccctagtttc tatcttagct
     2701 tcatcgcact tttgataatt aatttagctg tattggtttg gaggtatcgt caatgaagcc
     2761 ttatatacaa cttgttgtgt tcaagcaatg gttacaatac atcttgctcg taacaaccat
     2821 tgtcatcgca ctcgtactta ttggtatcgg ttaccgtgta gcacatgaca acttcaaaat
     2881 accgattacc attcaagatt tagaccaaac cactgcatca aaatcattcg tcaataaaat
     2941 taaacaatct gactatgtaa ctattaaaaa agtcgatgaa gatgaaagct atattgaaga
     3001 tgatgttact aaaaaggaag ctattttaag tatgcaaatt cctaaaggtt tctctcaaaa
     3061 attaaaagag aaccgtttaa aagaaacgat acagttatat ggtagagatg actttatagg
     3121 tggtattgct gtagaaattg ttagtagttc attatatgag cagcaaattc ctaacattat
     3181 ttatgaacac cttgaggata tgaaacagca tcaatccatc gatgctatca acaagtccta
     3241 tcataaacat acacctgaat ctaaaatcaa atttgtgtcg cttactaaac aagcacaaca
     3301 ctctatttca attagcttaa tctttgcggt gattttattt gttagcgctg ttcaagtagt
     3361 acttcattat cgtttaaacc aacaagcagc attgcaacga ttatcacaat atcatttaag
     3421 ccgtttcaaa ctatatagta cttatgtaat gacacatacg attttgttat tgttggtact
     3481 attggcagtt agtctatatt tgtctcaacc actcagctta atattttact taaaatcact
     3541 gttacttata ttgatttatg agataggtat cgtctttatc ttattccata ttcaaacaat
     3601 aagtcatcga ttattcatga catttatata tgcacttgct atgggtatcg tatacttgat
     3661 tattttcatg taaaggagcg taactgatga tagaaattaa taacctttca aagcgttacc
     3721 gtaacaaaca gattttcaat catttaacta tgtcctttga tagtaatcgt ttaaccgtat
     3781 tacttggtga taatggtgct ggaaaatcaa cattacttcg tatgattgct ggtattgaaa
     3841 aagctaatga tggaactatc aactatttcg gcgaaaaatg gaatcaaaga caaatacaaa
     3901 atcacatcgg ttatgtgcca caagacattg cgttatttga acacatgaca gtggctgaaa
     3961 acattaaatt ttttaaatca ctttgtaaaa atccaattaa cgatacaact atcaacgaat
     4021 atttacagca attaaacttt gatgatacgt ctgccaaagt atctacattg tccggtggga
     4081 ataaacgtaa aattaatata ttagtaggtt tactaggtca acctcgaatt ctcattttag
     4141 atgaaccgac agttggtatt gatttaaaat ctagacatga catccaccaa ctacttaaca
     4201 tcatgaaatc taaatgttta attatattaa ctacccatca tttagatgaa gttgaagcac
     4261 ttgcagatga tatcaagtta attggccaag atccttttta tcaacatgtt ttagaggaca
     4321 aacaatggac ttatacctat tattaaaacg aaaaaatccc aagctgcgta tgatatcgca
     4381 acttgggatt ttctgtatta tctactttgc aagtatgacg ttgggtctac tgcatattga
     4441 ttaccgatgc caccagacat acgttggaag tgtacgtgag gcgctgttga attacccgta
     4501 ctacctgaat atgcaatttg gtcaccagat ttgactttat caccagctga aacagttaaa
     4561 cgattattat gcatatacca ttggtagtta ttactgttcg cttctttaat cgttacttga
     4621 ttgccgccac catagttact ccaacctgct tgtactactg taccatcagt taatgagtaa
     4681 actggtgaat tttcaggcat tgcatagtcg acaccgtaat gcgcaccacc accgtgatat
     4741 tgtccatatg gttgtagttg tttacgactt gttaaccagc ttgcgtcttt cgcatgacca
     4801 ctagctgtcg ctttacttgc tgatccacca ttttgattag atgtgccatt aggataattg
     4861 acctttccat taccatcatg gctgttatac gcttggttgt tgttactatg tgaataatag
     4921 ctcgcgtctg gacctacatt tgattgataa ccatattgat taatatgctg ttggctttga
     4981 ctcgctgtgt agtcattgtt atctcctgct gttgctggat tcacatatgt ttggccgctt
     5041 ccattggcat ttgcattttt tggataacag ttataaaaat aatgcgtatg tccttgagca
     5101 tctacgaatg tatagctata ttctttatta tcaaacattg cttgattcca gttaccatca
     5161 ggtgtgtgat gataatcccc attagaatca attgtataat aagtaccata agatacgtct
     5221 tgtgattgtg ttgacatttg tgtatgtgct tgttgggtgt ttgtcgtttc tgctgcatct
     5281 gcttgatgcg ccattgtaaa tgtagcgaag cccatcgttg caatcgctgc tgctgttaat
     5341 tttttcatgt ataaaacatc ctccattaaa gttaaagtta gttttcaatt aaactgtact
     5401 gcacatacta aaagaattag acaactgagt aaaggattta attctcattt tccaactatt
     5461 taatattccc gaaatgtttt actaaactca ttacattgtc attacaaaat agccatacat
     5521 tgatattaaa atgacatctc tcactgcatt cgtttaacct tttataaatt ttcaaaatta
     5581 acaactaatc gttcgtccat gttcgcgatt caacgctaat gcatgatagt aatcatccat
     5641 caaatcatat caaccaaatt ccattatcaa tcgctattga ttgtcattca actttctaat
     5701 agtgatatgc ttctcaggct taaaaatcgt catatctttt ctattaatta aatcatctgt
     5761 gagctttaat gctactaatt cattgctgcc ataatactta atatataacg ttcttgtagt
     5821 taaatttatt acggtctgat acatcgtata gtgattgtca gcatcatgcg gacgtacaat
     5881 tccaatcggt atatttaccg catctaataa ataaaatgca ttcattaaat ccatttcttt
     5941 atcattgttt tgagcaatgt ttgctttcat aaatgccatt ctcacaaagc gctcagttga
     6001 agtaaatcca cctggcaatc caaatgtacc tgcttcattg cctaaaggtt caatcgttac
     6061 accttccaat aaatttgctg ttgctggata aggagaaata ttgatatatt gtcttaaatt
     6121 actataatgc caatttaagt ctggatgatt tgttaagaca ccaataggat tatcttttat
     6181 aaccacttca ccctctttaa atgaaacttc gactgtatgt ccagttgcat cggaaacatg
     6241 ataatgcaat ggcggaactt caccgatgtc atttaaatat acagctacaa catgtatttg
     6301 ggatgcttgt tgtttcatat cttcaatgct tgttgtatat cccaaaatcc atgtcacaat
     6361 ttcattttgc gtaatattca tcgcgtccgc tttgtgtgtt gatccatatg aactataacc
     6421 tcggaaatat tgtgttgaaa tggcaacgcc atgttcatta acaccatcac cataaataaa
     6481 accttccata tctgttcctg tgccaataaa gccatattgc gtttggcctg tcgtgccagt
     6541 gcgagatttc caacgataat ttctaggcgt cactgctggc gaaccatcta atggataatc
     6601 ataatccatc gtgcgtccaa gaagtacttg attatttaaa gtttgtattg tgaatcctgt
     6661 gcacattgtt ctcactcctc tgtaccttca tttactttaa tcactttcaa ataaagctgt
     6721 ttcacttaaa catactataa aaaatcaatt atacaagcaa ttaattgata ttcattctca
     6781 ataactgtgg tatgatatgt aaggaaatca tgacttatgt gtgagtgaac gatcatctat
     6841 acatccgttc acttcatctc atgactttct atatttaatt tttacaagga gtgacatctg
     6901 tgaataacac acaatcttca ccacgcagta atattattat tgcgattatg ttgtctgcat
     6961 taacatattg gttgtttgca caatcattta ttaatatagg acctctcgtt ggtcaaacgt
     7021 atcaaacctc tcctgccgtg ttaaatttat ctattagttt aacttccttc gccacaggta
     7081 tcttcatggt ggctgcaggt gatattgctg ataaaatagg acaactgaga atgacataca
     7141 tgggtctcat aatcagtatg tttgcatctc ttctattaat tatatcggac atcactgcac
     7201 tgctcatcat cggtagaatt ttacaaggtc tatcagcagc tatcttgtta ccttcaacag
     7261 ttggcgtgtt aaataatcaa tttaaaggag aacatttaag acgagcgatt agttatctaa
     7321 tgattagtac tgttggtggc atcggcctag ctggtgttat cggcggttta attgcctcaa
     7381 atttcggatg gcaaacgaat ttcatcatta gtatagtcat tgctttcatt gccatattgc
     7441 ttctaaaagg cacacctgaa aaagtaagtc aacatagcca ccgtcatcca ttcgattaca
     7501 aaggtatgtc gattttcgct gttatgattg gtagctttac attattgtta acacaaggat
     7561 tcgaacaagg ttggtttagt acattttcaa tcatttgtct gagcattttt atcatcacta
     7621 cgttgatatt catcatcatc gaacgtcgac atgaagtacc ttttattgat ttctcagtat
     7681 tacgcaaccg tccgttcatt ggtgcatttt taaataactt tgttttaaat agcggtctag
     7741 gcgtaacagt ggtctttttc atatatgctc aaacacacct tggtttatca gctgcgcaat
     7801 ctggacttgt tacattgcca tatgccattg tggcagttgc gatgattcgt ttaggtgaaa
     7861 aagcaacatt acgtttcggt ggcaaattga tgctcatcat tggtccgttg ttccctgtca
     7921 tcggcatcac tattattagc atgactcaat tattggcatc acaatatgtc attgcagtta
     7981 tcattggttt cgtcatatgt gcgataggta atggtttagt cgcaacacct ggacttacga
     8041 ttgcaatttt cagtatgcct aatgaaaaag ttggtttagc tacaggatta tataaaatga
     8101 gtggtacatt aggtggcgcc tttggtatag cactaagtac tacagttttc agtatgttac
     8161 aactaaacta tgcaccaagt gtagctgcaa ccgtaacatt tatagtcagc attgtattga
     8221 tgatccttgg ctcattgtct gcatacatga tcattccaaa aacagttaaa tcttaaatat
     8281 aatagaagaa ttatgtttcg aaatatcttt atcactttaa aatgatatac aagaaatcca
     8341 agaaaaataa gcgaactgaa taaataaaga ttcaattaac acatcagtat taggattcac
     8401 tctaaaacga ttaatagttt tataagaagg tgtttgatct tgagctaacc acatcattcg
     8461 aatactgtca tgaagtaatt tctctattct acgaccagaa aatacagatt gagtatatgc
     8521 atataagatg atttttaaca tcatctttgg atgataggat gttgcgccac gatgatgtct
     8581 gaattcatcg aatttgctat caggtatcgt ttcaacaatt tcattaacat gtcgcgaaat
     8641 atcattttga ggaattctaa cagaagtttt tattggtagt gtaagttggg caaagtgtct
     8701 tattttttta aagtattt
//
LOCUS       DAXWFK010000106         8703 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.106, whole genome shotgun sequence.
ACCESSION   DAXWFK010000106 DAXWFK010000000
VERSION     DAXWFK010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8703
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.106"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            7..2988
                     /locus_tag="ACGEXS_001874"
     CDS             7..2988
                     /locus_tag="ACGEXS_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFF8012873.1"
                     /translation="MKNAFKLFKMDLKKVAKTPAVWIILAGLAILPSFYAWFNLWAMW
                     DPYGNTGHIKVAVVNEDKGDTIRGKKVNVGNTMVNTLKKNKSFDWQFVSREKADHEIK
                     MGKYFAGIYIPSKFTHEITGTLRKQPQKADVEFKVNQKINAVASKLTDTGSSVVVEKA
                     NEQFNKTVTRALLEEANKAGLTIEENVPTINKIKNAVYSADKALPKINDFANKIVYLN
                     NHQADLDKYANDFRKLGNYKGDILDAQKKLNEVNGAIPQLNEKAKLILALNNYMPKIE
                     KALNFAADDVPAQFPKINQGLNIASQGIDQANGQLNDAKGFVTQVRSRVGDYQDAIRR
                     AQDLNRRNQQQIPQNSAANNETSNSAPAAGNGVASTPPSAPSGDTAPNNNVTQNTAPN
                     SNNAPVSTTPQSTSGKKDGQSFVDITTTQVSTANENTQNITDKDVKSMEAALTGSLLS
                     LSNNLDTQAKAAQKDSQALRNISYGILASDKPSDFRESLDNVKSGLEYTTQYNQQFID
                     TLKEIEKNENVDLSKEIDKVKAANNRINESLRLVNQLSNALKNGSSGTAEATKLLDQL
                     SKLDSSLSSFRDYVKKDLNSSLVSISQRIMDELNKGQTALSNVQSKLNTIDQVINSGQ
                     AILKNGKTRIDRLQTVLPSIEQQYISAVKNAQANFSKVKSDVAKAANFVRNDLPQLEQ
                     RLTNATASVNKNLPTLLNGYDQAVGLLNKNQPQAKKALSDLADFSQNKLPDVEKDLKK
                     ANKIFKKLDKDDAVDKLIDTLKNDLKKQAGIIANPINKKTVDVFPVKDYGSGMTPFYT
                     ALSVWVGALLMVSLLTVDNKHKSLESVLTTRQVFLGKAGFFIMLGMLQALIVSVGDLL
                     ILKAGVESPVLFVLITIFCSIIFNSIVYTCVSLLGNPGKAIAIVLLVLQIAGGGGTFP
                     IQTTPQFFQNISPYLPFTYAIDSLRETVGGIVPEILITKLIILTLFGIGFFVVGLILK
                     PVTDPLMKRVSEKVDQSNVTE"
     gene            complement(3100..4038)
                     /gene="manA"
                     /locus_tag="ACGEXS_001875"
     CDS             complement(3100..4038)
                     /gene="manA"
                     /locus_tag="ACGEXS_001875"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFF8012874.1"
                     /translation="MALFLQPVFKDRIWGGHALKAFNYDIPNETTGECWAISAHPNGP
                     NTIINGPYKDMTLDQLWSQHRELFDNDSRDSFPLLTKVLDANDKLSVQVHPDDDYALK
                     HEGELGKTECWYILDAAPGAEIIYGVHAHNKQTLIDMIDNHEFDSLFKRIPVKPGDFY
                     YVPAGTVHAIGSGILILETQQSSDTTYRIYDYDRRDQNGQLRDLHLEQSKDVIELGNH
                     EPNTTPITTHIDTHTVTQFVDNQYFAVYKWDIHGDLKFNKPHAYCLVTIIDGTGTLTV
                     NDKHYDIQKGSSFILTTEDEDIHFVGNISAIVSHPG"
     gene            complement(4052..6004)
                     /locus_tag="ACGEXS_001876"
     CDS             complement(4052..6004)
                     /locus_tag="ACGEXS_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFF8012875.1"
                     /translation="MKIVAITSCPNGIAHTYMAQEKLEQVAKEMGVDIKVETQGGVGA
                     ENVLTTQDIEEADGVIIAADKQVDLSRFVGKRLINENVREGIHNPRGLIQRIINQDAP
                     IYQSETNYHSKDRGKSKSGIQMVYQHLMNGVSFMVPFIVVGGLLIAIALTLGGETTSK
                     GLVIPDDSFWKSIENIGSLAFKFMVPILAGYIAVSIADKPGLVPGMIGGAIAADGSFY
                     GSDAGAGFLGGIVAGFLAGYIAKWIKDIKVPKAMAPIMPIIIIPIISSVVVGLIFIFL
                     IGAPISNIFEALTTWLKSMQGANIIILAMIIGAMIAFDMGGPVNKVAFLFGSALIAEG
                     NYAVMGMVAVAVCTPPIGLGLATFVQKYKFNHSEREMGKASFTMGLFGITEGAIPFAA
                     QDPLRIIPANIIGAMIASVIAAIGGVGDRVAHGGPIVAVLGGIDHVLWFIFAVIVGSL
                     VTMTTVLLLKRNTPVIAVDAPAQHTQLHDTDITQHDTEVDNVDNTAKHSHLNKPSHVF
                     DQQTMIITDHDMSRNEAIDMLIHKLKICRYVEHTSHLKNAILEREMESTTAIGMNVAI
                     PHAKSDVVKQPIVAVMKNNHGVKWDSLDGSLPQLIFLIAVPNNSQDTHLKILQRLSKA
                     LMNDETRQSLINANSTTEIYNLLMKI"
     gene            complement(6081..7955)
                     /locus_tag="ACGEXS_001877"
     CDS             complement(6081..7955)
                     /locus_tag="ACGEXS_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFF8012876.1"
                     /translation="MLDRHLKLLQFFIKNPSKHISSNEIAKHVNVSNRTVRNDIHVIN
                     SNFMDDIIVSIKSKGYQLNTSQYTLETITERYTHIQSYKEKLLLSMAYQLLMHNKSQT
                     LQQLEQDYLLSKTVLNDYFVRIQQWCQKFNIALTIKKKQGIVVDGTDNDITNAIIHLN
                     QLSSGHVHVEDLILNELPDSHQRMISHIIQETLQQHSIETTDIQIQQLLIHLILIIKR
                     KQSLSELDTLNHDSKLISQSCIKQINERLGYDLNEKVINMFSFFIAYHFNKLDIGIER
                     IFIQSYIDRLIELMQQRIHVPFNEDSILQQNLYNHFSKAYLRITQNIYLNNPLVIEIK
                     KLYPFVFNTLFEAIDKLAIDTDIEMSEDEIAFLTIHFQAAIERRTKTQLNVVIACYYG
                     LGVSNFLETKINNLSEELSVINTIKLENITHYHFDNVDLLITTHDIPKQTLNILPKHL
                     TTIKVAPLFSEDDRHKIRHVVKQKQNPVQAHHHMDTVNFLVVNTEQKSRHTVQIFEEA
                     QKILQAHHAIVEGYIESALEREKSSSTYIGNFMAIPHGDPEKVLQSHVLIFRTKDVFP
                     WRQHDVKLVFFLAISHKDKAFTKQMMQLIANLDDDSVNHLCSLDDHSLKQQLFEYLQE
                     "
     gene            complement(8195..8653)
                     /locus_tag="ACGEXS_001878"
     CDS             complement(8195..8653)
                     /locus_tag="ACGEXS_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012877.1"
                     /translation="MKKLLITIVIIVLIGALGFAIYAAINHSTSSSNNQEQENKSTHK
                     KTETEENDQQDDSADKTDKNQNNGNNVTNDNQPSSPSNVPNNHSTVPSNTQPAQPKDS
                     NSNKNNTHGGGSQSSTNNQNNQQQNTPPANKSNNTNTPSKQTPQAQSPKN"
BASE COUNT         2858 a         1429 c         1582 g         2834 t
ORIGIN      
        1 gtgacaatga agaacgcatt taaattattt aaaatggatc tgaagaaagt agctaagacg
       61 ccagctgtgt ggattatctt agcaggctta gctattttgc catcgttcta cgcttggttt
      121 aacttatggg caatgtggga tccatatggc aacacgggac acatcaaggt cgcagtcgtt
      181 aatgaagata aaggcgacac aatcagaggg aaaaaagtta atgtcggtaa tacgatggtt
      241 aatacactca agaaaaataa aagttttgat tggcagtttg taagtagaga gaaagctgat
      301 catgagataa aaatgggtaa atattttgca ggtatttaca tcccatctaa gtttacacat
      361 gaaattacag ggacactacg taagcagcct caaaaagcag atgtagaatt taaggtgaat
      421 cagaagatta acgctgttgc gtctaagcta acagatactg gttcgtcagt tgtcgttgaa
      481 aaagcgaatg aacaatttaa taaaacagta actcgagcat tattagaaga agctaacaaa
      541 gcaggtttaa ctattgaaga aaatgtgccg acaattaaca agataaaaaa tgcggtatat
      601 tcagcagata aagctttacc taagattaat gactttgcga ataaaattgt atatttgaat
      661 aaccaccaag cggatttaga taaatatgcc aatgatttta gaaaactagg aaattataaa
      721 ggtgatattt tagatgctca gaaaaaatta aacgaagtca atggtgctat tccgcaactt
      781 aatgaaaagg ctaagttgat attagcttta aataattata tgccgaaaat tgaaaaagcg
      841 ttaaattttg cagctgatga cgtgccagcg cagttcccta aaattaatca aggacttaac
      901 attgcgagtc aaggtattga tcaagctaat ggacagttaa atgatgccaa aggcttcgtc
      961 acacaagtta gaagtagagt cggtgattat caagatgcaa ttcgacgcgc gcaagattta
     1021 aatcgaagaa accagcaaca gattcctcaa aatagcgcgg cgaacaacga aacatcaaat
     1081 agtgcacctg cagctggtaa tggtgtagca tcaacgccac caagtgcacc aagtggcgat
     1141 actgcaccaa ataataatgt tacgcaaaat accgcaccaa atagtaataa tgcgcctgta
     1201 tcgactacac cacaaagtac aagcgggaaa aaagatggtc aaagttttgt agatataaca
     1261 acaacacaag tcagcacagc taacgagaac acacaaaaca ttacagataa agatgttaaa
     1321 tcaatggaag cggcattaac gggctcttta ttatcattat caaataattt agatacccaa
     1381 gcgaaagccg cacaaaaaga tagtcaggca ttacgtaata tttcgtatgg gattttagca
     1441 tcggacaagc cttctgattt tagagagtct ttagataatg ttaagtccgg tttagaatac
     1501 acaacgcaat ataatcaaca atttatcgat acattaaaag agattgagaa gaatgaaaat
     1561 gttgatttat caaaagaaat tgataaggta aaagcagcta ataatcgaat taatgaatca
     1621 ttaaggttag ttaatcaatt aagcaatgca ttaaagaatg gtagttcagg aactgctgaa
     1681 gctactaaat tactagatca actttcaaaa ctagattcat cattatcatc atttagagat
     1741 tatgttaaaa aagatcttaa cagctcttta gtatcaatat cacaacgtat tatggatgaa
     1801 ttgaacaaag ggcaaactgc attatccaat gttcagtcta aattaaatac aattgatcaa
     1861 gtcattaaca gtggacaagc tattttaaaa aatggtaaaa cacgtattga tcgcttacaa
     1921 acagtattac caagtattga acaacaatac attagtgctg ttaaaaatgc tcaagcaaac
     1981 ttctcgaaag tgaaaagtga tgtagctaaa gctgctaact ttgtgcgcaa tgacttacca
     2041 cagttagaac agcgattaac taatgcgaca gcaagtgtga ataaaaattt accaacgtta
     2101 ttaaatggtt atgatcaagc ggtaggatta ctaaataaaa atcagccaca agcgaaaaag
     2161 gctttatcag atttagctga tttttctcaa aataaattgc ctgatgttga aaaagatttg
     2221 aaaaaagcga ataaaatttt caagaaatta gacaaagatg atgcagtcga caaattaatc
     2281 gacacactta agaatgattt gaaaaagcaa gcgggtatta ttgcaaatcc tattaataag
     2341 aagactgttg atgttttccc agttaaggat tatggttcag gtatgacacc attctatact
     2401 gcactgtcag tatgggtagg tgcactcttg atggtaagtt tattaacggt tgataataaa
     2461 cataagagtc tagagtcagt cttaacgaca agacaagtgt tcttaggtaa ggcaggattc
     2521 tttataatgc ttggtatgtt gcaagcactc attgtatcgg ttggagattt gttaatccta
     2581 aaagcaggag ttgagtcacc tgtattattt gtacttataa cgattttctg ttcgattatt
     2641 ttcaactcaa tcgtatatac gtgcgtatca ttacttggta acccaggtaa agccattgca
     2701 atcgtattgc ttgtattaca aattgcaggt ggtgggggaa cattcccaat tcaaactacg
     2761 ccacaatttt tccaaaacat ttcgccatac ttaccattta cgtatgcaat tgattcatta
     2821 cgtgaaacag taggcggtat tgttccggaa atcctaatta caaaattaat tatattaacg
     2881 ttatttggta taggattctt cgttgtaggt ttaattttaa aacctgtaac agatccattg
     2941 atgaagcgcg tatctgaaaa agttgaccaa agtaacgtta cagaataaaa attaaatcca
     3001 cacattaggg ttatagctcc ttaatgtgtg gatttttatg tttttagaca gaagagatag
     3061 taatttctgt cttttatggg acggttgtta tcattgctat tatccaggat gacttactat
     3121 agcactaata ttaccgacaa agtgaatatc ctcgtcttcc gtagttaaaa taaagctaga
     3181 acctttttgg atgtcatagt gcttatcgtt tactgttaaa gtaccagtac catcgataat
     3241 tgtaactaag caataagcat gtggtttatt gaattttaaa tctccatgaa tatcccattt
     3301 atatactgca aaatattgat tatctacaaa ttgagttaca gtgtgtgtgt cgatgtgagt
     3361 tgttatagga gtagtatttg gttcatgatt gcctaattca atcacatctt tactttgctc
     3421 taagtgcaaa tcacgcaatt gaccattttg atctcgtcta tcatagtcat aaatacggta
     3481 tgtcgtatcg gaggattgtt gtgtctctaa aattaaaata cccgaaccaa tggcatggac
     3541 agtgccagca ggaacataat aaaagtcacc gggcttaaca ggtatacgtt tgaaaagact
     3601 gtcaaattca tgattatcaa tcatgtctat taacgtctgt ttattatgtg catgtacgcc
     3661 atatataatt tcagcacctg gggctgcatc taaaatatac caacattctg ttttacctag
     3721 ttcgccttcg tgttttaaag cgtagtcatc atctgggtga acttgaacag ataatttatc
     3781 attggcatct aatactttag ttagcagagg gaaactatct cgtgaatcat tatcgaataa
     3841 ttcacgatgt tgtgaccaaa gttgatctag ggtcatatcc ttgtatggac cattgataat
     3901 tgtattagga ccatttggat gtgcagaaat tgcccagcat tcaccagttg tttcattagg
     3961 gatatcatag ttaaatgctt ttaatgcatg accgccccaa attctgtctt taaaaacggg
     4021 ttgtaaaaat aatgccatag ttaaaactcc tctatatttt cattaataag ttataaattt
     4081 ctgtagtact gtttgcatta attagtgatt ggcgtgtctc atcattcatt aacgctttag
     4141 ataagcgctg aagtattttt aaatgtgtat cctgactgtt gtttggtacg gcaattaaga
     4201 atatcaattg aggtagacta ccatctagac tgtcccattt aacaccatga ttatttttca
     4261 taacagctac aatcggttgt tttacaacat cagactttgc atgtggaatg gccacgttca
     4321 tgccaatagc tgtcgtagac tccatttcac gttctagtat tgcatttttt aaatgcgatg
     4381 tgtgctctac ataacggcaa attttaagtt tatgaatcaa catatcaatt gcttcgtttc
     4441 gagacatgtc gtgatcagta attatcatag tttgttgatc aaaaacatga gaaggtttat
     4501 tgagatgtga atgtttcgcg gtgttatcta cattgtcaac ctctgtatca tgttgtgtaa
     4561 tatctgtatc atgaagttgc gtgtgttgcg ctggtgcatc tactgctata actggtgtat
     4621 tgcgttttaa taatagtaca gtagtcattg tgacaagact acctactatc actgcaaaga
     4681 taaaccataa tacatgatca ataccaccta atacagccac gattggacct ccatgtgcga
     4741 ctctatcgcc gacaccacca atggctgcaa tgactgatgc aatcattgca ccaatgatgt
     4801 ttgcaggtat aatgcgcaat ggatcttggg ctgcgaaagg aatagcacct tcagtaatac
     4861 caaatagtcc catagtgaag gaagccttac ccatttctct ttcggaatga ttgaatttat
     4921 acttttgaac aaacgttgct aaacctaaac cgattggtgg tgtacataca gcaactgcga
     4981 ccatacccat aacggcgtaa ttaccttcag caataagtgc tgagccaaat aaaaatgcta
     5041 ccttgtttac tggaccgccc atatcgaagg caatcatcgc acctataatc atcgcaagta
     5101 taataatatt agcaccttgc atacttttta accaggttgt taatgcctca aaaatattag
     5161 aaattggtgc accgattaaa aatataaata tcaatcctac aacgaccgat gaaataatgg
     5221 gaataataat gataggcata attggtgcca ttgcttttgg aactttaata tctttaatcc
     5281 actttgcgat ataacctgct aagaaaccag caacaatacc acctaaaaat cctgcgcctg
     5341 catcactgcc ataaaaacta ccgtcagcag cgatagcgcc gccaatcata ccaggaacaa
     5401 gaccgggctt gtcagcgata ctaacagcga tatatccagc tagtattgga accataaatt
     5461 taaaggctaa actaccaatg ttttcaatgg atttccaaaa tgaatcatct gggatgacta
     5521 atccttttga tgtcgtttca ccgcctagag tcagcgcgat ggcgataagg agtccaccaa
     5581 ctacgataaa aggaaccata aacgatacac cgttcattaa atgttgatac accatttgaa
     5641 taccactttt agacttaccg cgatctttcg aatgataatt tgtttcagat tgataaatag
     5701 gcgcatcttg attaatgata cgttgaatta gacctctcgg attatgaatc ccttcgcgaa
     5761 cattttcatt aatcaaccgt ttaccaacaa atcgggacag atcaacttgt ttatcagctg
     5821 caattatgac accgtcagct tcttcgatgt cttgcgtagt taaaacattt tcagcaccaa
     5881 caccgccctg tgtctctact ttaatatcca cacccatttc ttttgctacc tgctcaagct
     5941 tttcttgagc catatatgta tgtgcaatgc catttgggca tgaggtaata gctacaattt
     6001 tcataaaatc atctcctttt ctatattgta agcgtattct cgatactaaa aaaaagaata
     6061 attaccgtta ctagtggcaa ttattcttgt aagtattcaa ataactgttg ctttaaacta
     6121 tgatcatcta aactacataa atggttcact gaatcatcat ccaagttagc aattaattgc
     6181 atcatttgtt ttgtaaaagc tttgtcttta tgcgaaatcg ctaagaaaaa gacaagtttg
     6241 acatcgtgtt gtcgccaagg aaaaacatct tttgtgcgaa aaataagcac atgtgattgt
     6301 aaaacttttt caggatctcc atgaggaatc gccataaaat tacctatgta tgtagaagat
     6361 gatttctcac gctctaaagc tgattcgata tatccttcta caatcgcatg atgtgcttgt
     6421 aatatttttt gagcttcttc aaaaatttgc acagtatgcc gtgatttttg ttcagtattt
     6481 acgacaagga aattgacagt gtccatatga tgatgtgctt gaaccggatt ttgcttttgc
     6541 ttcacaacgt gtctgatttt gtgacgatca tcttcagaaa ataatggtgc aaccttgata
     6601 gtcgtcaggt gcttaggaag tatgtttagc gtttgtttag gaatatcatg ggtcgttatt
     6661 aataaatcta cattgtcaaa gtgatagtgt gttatatttt ctagtttaat cgtatttatc
     6721 actgacaact cttcggataa gttatttatt ttagtttcta aaaaattcga cacacctaga
     6781 ccataataac aagcaatgac tacatttaat tgtgttttgg tacgacgctc gatggcagct
     6841 tgaaaatgaa ttgttaaaaa tgcaatttca tcttcgctca tctctatatc agtatcaatt
     6901 gctaatttat caatcgcttc aaaaagtgtg ttaaacacaa agggatagag ttttttaatc
     6961 tctataacta aaggattgtt taaataaatg ttttgagtga tacgtaaata tgctttacta
     7021 aaatgattat ataaattttg ttgtaaaatc gaatcttcat tgaaaggtac atgaatacgt
     7081 tgctgcatca attcgattaa gcgatcaata taactttgta taaatatacg ttctatgcca
     7141 atatcgagtt tattaaaatg ataagcaata aagaatgaaa acatattgat tactttttcg
     7201 ttcaagtcat aacctaatct ttcgttgatt tgcttaatgc aagattgaga tatcaatttt
     7261 gaatcgtggt ttaacgtgtc tagctcactt aaagattgct tacgtttaat gattaaaatg
     7321 agatgaatta aaagctgttg tatttgaata tcagttgttt caatactatg ttgttgaagt
     7381 gtctcttgta taatatgcga aatcatcctt tggtgtgaat caggtaattc atttaaaatt
     7441 aggtcttcaa catgtacatg ccctgatgat aattgattta aatggatgat ggcattagtg
     7501 atatcattat ctgttccatc gaccacaata ccttgttttt tcttgattgt taaagcgata
     7561 ttaaattttt gacaccattg ttgaattcga acgaaatagt cgttgagcac agttttagat
     7621 aatagataat cttgctctag ttgttgcaat gtctgcgatt tattgtgcat tagcagttga
     7681 tacgccatag ataataatag tttttcttta tatgattgaa tatgcgtgta acgttcagtt
     7741 atcgtttcta aggtatactg agatgtattt aattgatatc ctttagattt aatacttacg
     7801 atgatatcat ccataaaatt gctattgata acatggatat cattgcgaac tgtgcggttt
     7861 gaaacattga catgtttagc aatttcatta gaactaatgt gctttgatgg atttttaata
     7921 aaaaactgga gtagttttaa gtgtctatca agcatttaac atgtacctcc tttctaaatt
     7981 tttcgtgtaa gcgtttttaa gggtgtatta atattattaa acatgagagc ttatacatac
     8041 gtcaatgaca ttaaagcgaa cttttatatg attttaacag agtgcgaatt atgcaaataa
     8101 agaacagcag taagatattt caaatagaaa aatatctcac tgctgttttt ctgaatttat
     8161 gcatctggta cttgtggacg tatcaggcaa atgattaatt tttaggtgat tgtgcttgag
     8221 gtgtttgttt agaaggcgta ttggtattat ttgatttatt agcaggtggt gtgttctgtt
     8281 gttgattatt ttggttgttt gttgacgatt gactgccacc accgtgagta ttatttttat
     8341 ttgaatttga gtcttttggc tgagctggtt gcgtattcga tggtactgtt gaatggttgt
     8401 taggaacatt tgacggtgaa gatggttgat tgtcattagt aacgttattg ccattatttt
     8461 gatttttatc tgttttgtct gcactatcat cttgttgatc attttcttcg gtttctgtct
     8521 ttttatgcgt agatttattt tcttgttctt ggttattact tgatgatgta gaatgattta
     8581 ttgcagcata aattgcgaac ccaagtgctc cgattaatac gataattacg atggtaataa
     8641 gaagtttttt caaatataac gcctccatgt taatgaaaag tagttgatac gtgaagctaa
     8701 ata
//
LOCUS       DAXWFK010000107         8666 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.107, whole genome shotgun sequence.
ACCESSION   DAXWFK010000107 DAXWFK010000000
VERSION     DAXWFK010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8666
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.107"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(109..1275)
                     /gene="norA"
                     /locus_tag="ACGEXS_001879"
     CDS             complement(109..1275)
                     /gene="norA"
                     /locus_tag="ACGEXS_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorA"
                     /protein_id="HFF8012878.1"
                     /translation="MNKQIFVLYFNIFLIFLGIGLVIPVLPVYLKDLGLTGSDLGLLV
                     AAFALSQMIISPFGGTLADKLGKKLIICIGLILFSVSEFMFAVGHNFSVLMLSRVIGG
                     MSAGMVMPGVTGLIADISPSHQKAKNFGYMSAIINSGFILGPGIGGFMAEVSHRMPFY
                     FAGALGILAFIMSIVLIHDPKKSTTSGFQKLEPQLLTKINWKVFITPVILTLVLSFGL
                     SAFETLYSLYTADKVNYSPKDISIAITGGGIFGALFQIYFFDKFMKYFSELTFIAWSL
                     LYSVVVLILLVFANDYWSIMLISFVVFIGFDMIRPAITNYFSNIAGERQGFAGGLNST
                     FTSMGNFIGPLIAGALFDVHIEAPIYMAIGVSLAGVVIVLIEKQHRAKLKEQNM"
     gene            1484..1906
                     /locus_tag="ACGEXS_001880"
     CDS             1484..1906
                     /locus_tag="ACGEXS_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFF8012879.1"
                     /translation="MKLQKSNHTILLVLEKGEDIVECITTFADDQDLTFTSVSGIGAC
                     DDVVLKFFNLTTKQYEEKHITEPLELTSLLGNISRLDNGHFAHLHATFGTQSYETFSG
                     HLAKAIVSATAEIILTLTDLDIQRSFKDAVGLNLLDPQ"
     gene            2092..2709
                     /locus_tag="ACGEXS_001881"
     CDS             2092..2709
                     /locus_tag="ACGEXS_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HFF8012880.1"
                     /translation="MQSRYIARIYFLILFIVSLFETKIFQFMTLNLFLAYIPFELSLL
                     LRLFKPNKKYEWPLFIIYSFIFVLLLPNTFYMVTDLIHLNQFQFNFYAGLNLSEWKYF
                     TYLLLGVFLAVYVMILIYFEILTLTSHRWLNRLIVVVLMFLNGFGIYIGRFLRLHSVY
                     FFNEPLHVLNHILESLTLKTAMFVCFMVVMQAAILLFGKGVRLNK"
     gene            2706..2990
                     /locus_tag="ACGEXS_001882"
     CDS             2706..2990
                     /locus_tag="ACGEXS_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012881.1"
                     /translation="MTINIIVLIVSIIVFQLIIGHIWHDIGLSYLRSILLMMLPFGLG
                     VFIQQVSYYERQYPKWQVPQNIKVRLKYIYLATFLEYVVLYLTLFTDILR"
     gene            complement(3145..4518)
                     /locus_tag="ACGEXS_001883"
     CDS             complement(3145..4518)
                     /locus_tag="ACGEXS_001883"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFF8012882.1"
                     /translation="MAIAVLLNRMFRMEHNPLFEYIYQQKEDIDACYFIIPEEDMSSA
                     SDLKAQFYRGTLQRFYQSLHAEKLTPYVMSYDDIISFCKENNISEVVTAGDIMSYHLE
                     EYDILHQRSLFNEARIAVTLIRGNHYFKASKTMNQQGEPYNVFTSFYKKWRPYLRHRD
                     VYHYDLKSFEDFVIASPDDLVFDDIAFGSSQIIEQNKWQHFLDQDIQNYESGRDYLPE
                     VLTSQLSVALAYGLLDIIEIFNDLLARYDEDEANYEAFIRELIFREFYYVLMTQYPET
                     SYQAFKPKYRQIKWSQNEADFNAWCEGQTGFPIIDAAIMELTQTGFMHNRMRMVVSQF
                     LTKDLFIDWTWGEKFFRKHLIDYDAASNIHGWQWSASTGTDAVPYFRMFNPIRQSERF
                     DAKALYIKTYLPIFNQIDAKYLHDTQRNESNLFEQGIELGSHYPRQMVDHQEKRTQVL
                     ATFKALD"
     gene            complement(4604..6157)
                     /locus_tag="ACGEXS_001884"
     CDS             complement(4604..6157)
                     /locus_tag="ACGEXS_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HFF8012883.1"
                     /translation="MSEEKHVVEHEQQKKEKTKKQYKPFWIVMSFIILIVVLLLPAPS
                     SLPIMAKAVLAILAFAVIMWVTEAVSYPVSATLIIGLMILLLGFSPVQNLGEKLGNPK
                     SGSAILAGSDLLGTNHALSLAFSGFATSAVALVAAALFLAAAMQETNLHKRLALLVLS
                     IVGNKTRNIVIGAIIVSIVLAFFVPSATARAGAVVPILLGMIAAFKVSKDSKLASLLI
                     ITSVQAVSIWNIGIKTAAAQNIVAINFINHQLGFDVSWGEWFLYAAPWSIVMSVALYF
                     IMIKVMPPEINTIEGGKDLIKEELHKLGPVSPREWRLIVISMLLLLFWSTEKVLHPID
                     SASITIIALGVMLMPKIGVMTWKHVENKIPWGTIIVFGVGISLGNVLLKTGAAQWLSD
                     QTFGVLGLKHLPIIATIALITLFNILIHLGFASATSLSSALIPVFISLTSTLHLGDQS
                     IGFVLIQQFVISFGFLLPVSAPQNMLAYGTGTFTVKDFLKAGIPLTIVGYILVIVFSM
                     TYWKWLGLL"
     gene            complement(6440..6727)
                     /locus_tag="ACGEXS_001885"
     CDS             complement(6440..6727)
                     /locus_tag="ACGEXS_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012884.1"
                     /translation="MTNEDKRFEQLRFERKFIVIPYLIYAVIVLLLNIFYSDLKITMT
                     LFGLFFAYNVVILFIAFVKHYKRTLLLSLILTVLSGAAFFGIIYVYGINHF"
     gene            complement(6762..7670)
                     /locus_tag="ACGEXS_001886"
     CDS             complement(6762..7670)
                     /locus_tag="ACGEXS_001886"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFF8012885.1"
                     /translation="MEQIMINHYVHFSRLVQGFWRANEWKMTAKELNYFINELVERGI
                     TTMDHADIYGDYQCESLFGNALDLSPELRNKIQIVTKCGIILPSKQFDFTNGHRYDLS
                     SKHIVKSVEQSLINLNVDYLDSLLIHRPSPLMDPEQVADALTKLVKQGKLKSFGVSNF
                     NHSQYQLLNQYIMKERLHISINQLELSPYHVDSLQDGTMDSMYQNHVQIMAWSPFAGG
                     KIFDKEDIKAQRIMKVVQSIADKYGVSDTAVMIAWLVKIPHRIMPILGTSQLKRIDQA
                     IEGLQLNLDDQSWFDIYTAIIGQDIP"
     gene            complement(7773..>8666)
                     /locus_tag="ACGEXS_001887"
     CDS             complement(7773..>8666)
                     /locus_tag="ACGEXS_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFF8012886.1"
                     /translation="SIINGFLGSGKTTLLTHYISELLKNDEKIKIIMNEFGTFDIDSN
                     SISNEIEVHSLINGCVCCDLKQELVYELKAIALKGDVNHVIIEATGIAHPLELLVACQ
                     DPQIVNFFEKPIIYGVLDATRFLERHQYTENTVSLMEDQLKLSDMIIINKIDLITDDS
                     LEKIDKQLGMICASIPTYKTTYGKVSLEELDLTVKDREISSHHHHHHGIKSMTYTFTG
                     PIDRHLFYQFIMKLPESVLRLKGYVSFRDQPNAIYEFQYAYGLPDYGIIGMQLPLTIV
                     IIGETLDTNHIRNQLDMLQFT"
BASE COUNT         2966 a         1552 c         1218 g         2930 t
ORIGIN      
        1 ttaaatgtaa agcatcgaat tttcttaata aaattcataa gaaaaacgat gctaatcatt
       61 cataatatta cgctttactt tatatcaata tacaccaaaa tacttatgct acatattttg
      121 ttctttcaat tttgctctat gttgcttttc aattaaaaca ataacaacac ctgctaatga
      181 aacacctata gccatataaa ttggtgcttc aatgtgtaca tcaaataacg cacctgcgat
      241 taaaggacct atgaaattac ccatactagt gaatgtcgag ttcaatccgc ctgcaaagcc
      301 ttgcctttct ccagcaatat tagaaaaata atttgtaatg gctggtcgta tcatatcaaa
      361 acctatgaag acaacaaaac tgattaacat tattgaccaa tagtcattag caaaaactaa
      421 taatattaag acaacaactg aatataataa tgaccaagct ataaatgtta actctgagaa
      481 atacttcata aatttatcga agaaatagat ttggaaaagt gccccaaata taccgccacc
      541 cgtaatagca atcgaaatat ctttaggtga ataatttacc ttgtcagctg tgtatagtga
      601 atacaatgtt tcaaatgcag ataaaccaaa cgataataca agtgttaaaa taactggtgt
      661 aataaacact ttccagttaa ttttcgttag caattgtggc tctaactttt ggaaaccact
      721 tgtcgtagac tttttcggat cgtgaatcaa tacaattgac attataaatg ctagaatacc
      781 taatgctcct gcaaagtaaa atggcatacg atgtgaaact tctgccataa atccaccaat
      841 ccctggtcct aaaatgaatc cagaattgat aatcgctgac atgtagccaa agttttttgc
      901 tttttgatgg cttggtgaaa tgtcagctat taaacctgtc acaccaggca ttaccatacc
      961 agcactcata ccaccaatca ctctcgataa catcaatacc gaaaaattgt ggccaactgc
     1021 aaacataaat tctgacactg aaaacaaaat taatcctata catataatta atttcttccc
     1081 taatttgtca gctagcgtac caccaaacgg cgatataatc atttgagata acgcaaaagc
     1141 agcaactagt aatcctaaat cactaccagt taatcccaaa tcttttaaat aaacaggcaa
     1201 gactggtatt actaaaccga tacctaaaaa aatcaagaaa atattaaaat ataagacaaa
     1261 aatctgttta ttcatatgct cacctctttt ttctccatgt catgcttaaa gctgttattc
     1321 ttcttttcca cgacagattg caattcatgc taaatattcg taaaaagttt ctatattgta
     1381 ttacaacatt gctacatttg acaatatttt ttctttgtaa ttatcactat tttccattta
     1441 attgtataat taatgacata ttaaaataaa ggggattgat catatgaaat tgcaaaaaag
     1501 taatcatact atactactag tgttggaaaa aggagaagat atcgttgaat gtataacaac
     1561 ctttgcagat gaccaagact taacatttac atctgtcagc ggcattggtg catgtgatga
     1621 cgttgtatta aaatttttca atttaacaac gaaacaatac gaagaaaaac atattactga
     1681 gccactggaa ttgacgagct tattaggtaa tatttctcgt ctagacaatg gtcattttgc
     1741 acatttgcat gctactttcg gtactcaatc atatgaaact tttagcggac atctcgctaa
     1801 agcaattgtt tctgcaactg ctgaaatcat tcttactcta actgatttgg acattcaacg
     1861 ttcattcaaa gatgcagtcg gtttaaattt acttgatcct caatagcact ttacttttta
     1921 gttgaatcaa attttattac aagttatcat aagtgcttaa tcataaatgc attctcgttt
     1981 attaatatac ttcaacgagt taaattaaat ttttaattag gaattgaatg cgtttataac
     2041 tatcacattt gaatgaataa ctttttcatt agaaagtagt gtttattatt gatgcaatct
     2101 cgatacatcg ctagaattta ctttttaatt ttatttatcg tctccctttt tgagactaaa
     2161 atttttcaat ttatgacttt aaatttgttt ttagcatata taccttttga gcttagtcta
     2221 ttattacgat tattcaaacc gaataaaaag tatgaatggc cactctttat catatatagt
     2281 tttatatttg tgttactttt accaaataca ttttatatgg taactgattt aatccatttg
     2341 aaccaatttc aattcaactt ttacgcagga ctaaatttaa gtgagtggaa atactttacc
     2401 tatttactgc ttggtgtctt cttagcggtt tatgtgatga ttttaatcta ttttgaaata
     2461 ctaacattga cttctcaccg ttggcttaat agattaattg ttgtcgtgct aatgttttta
     2521 aatggattcg gaatttatat tggtcgtttc ttacgcttac attccgttta tttctttaat
     2581 gagcccttac atgttctaaa tcacattcta gaaagtctaa cgcttaaaac tgctatgttt
     2641 gtatgtttta tggttgtcat gcaagcagca attctattat ttggaaaagg agtgcgtttg
     2701 aacaagtgac aataaatata atcgttttaa tagtatctat cattgtcttt caattgatta
     2761 ttggacacat atggcatgat attggtttaa gttatttaag aagtatctta ctcatgatgc
     2821 tgccatttgg actaggtgta tttatacaac aagtgtcgta ttacgaacga caatatccta
     2881 aatggcaagt gcctcaaaac ataaaagtta gattgaaata tatttatctc gctacgtttt
     2941 tagagtatgt cgtactatac ctcactttat ttacggacat actaagataa catcaaatta
     3001 ataagaaaac taaatacaac ttatatattt gaggaaagcc aatttctatc acatcaataa
     3061 tagaaattgg cttatttata ttgcttaaga actttatatt ctgctccaga ttttactcat
     3121 gccagatcta tcaattgagc cgaattagtc tagcgcttta aatgtagcta aaacttgtgt
     3181 acgtttttct tgatgatcta ccatttgtct tggataatga ctacctaatt caatcccctg
     3241 ttcaaaaagg ttggactcat tgcgttgtgt atcatgcaaa tattttgcat caatttgatt
     3301 aaaaatcgga agatatgttt tgatatacaa agctttagca tcaaagcgtt cactctgtct
     3361 tattggatta aacattctaa aatacggcac tgcatccgta cctgtagaag cagaccattg
     3421 ccatccatga atatttgatg ctgcatcata gtcaataagg tgctttctaa agaatttttc
     3481 tccccatgtc caatctataa ataaatcttt ggttaaaaat tgcgacacaa ccattctcat
     3541 tcgattatgc ataaaaccag tttgtgtcaa ttccattatt gctgcatcaa tgattggaaa
     3601 tcctgtttgc ccttcgcacc atgcattaaa atccgcttca ttttgcgacc attttatctg
     3661 tcgatattta ggtttgaaag cttggtatga ggtttcagga tactgtgtca ttaacacata
     3721 ataaaattct ctaaaaatga gttcacgtat aaatgcttca tagtttgcct catcttcatc
     3781 ataacgcgcc aataaatcat taaaaatttc aataatatct aataatccat atgctaaagc
     3841 aacacttagc tgacttgtta atacttcagg taaatagtct cttccgcttt cgtaattctg
     3901 tatatcttga tctaaaaaat gttgccattt attctgttca attatttgtg aggatccaaa
     3961 tgctatgtca tcaaacacta aatcatcagg tgatgcaatg acaaagtctt cgaatgattt
     4021 taaatcatag tgatatacgt ctctatgcct caagtaaggt cgccattttt tatagaaact
     4081 agtaaaaaca ttgtatggct ccccttgttg attcattgtt ttactcgctt taaagtaatg
     4141 attcccacgt atcaaagtaa cggcaatgcg tgcttcattg aataaagaac gttgatgtaa
     4201 aatatcatat tcttcaagat gataactcat aatatcaccc gcagtcacta cttcagagat
     4261 attgttttct ttacaaaatg aaatgatatc gtcataagac ataacataag gtgtaagctt
     4321 ttctgcgtgc aacgattggt aaaagcgctg caaagtaccg cgataaaact gtgctttcaa
     4381 atcagaagct gaagacatgt cctcttccgg aatgataaaa taacatgcat caatgtcttc
     4441 tttttgttga taaatatatt caaataatgg attgtgttcc attcgaaaca ttcgatttaa
     4501 taacacagca attgccataa acaaaacacc cttcaattat ctttttattt tattaagctt
     4561 tttgtcactt taaaataacc tagattctta tttatatttt taattaaagc aaacctaacc
     4621 atttccaata agtcatgcta aaaactatca ctagaatata ccctacaatt gtcaatggta
     4681 tacctgcctt caagaaatct ttaaccgtaa aagtaccagt gccataagcc aacatatttt
     4741 gaggtgcact aacaggtaat aagaaaccaa aactaataac aaattgttga attaaaacaa
     4801 atcctataga ctggtctcct aagtgtaacg tagaggttag cgaaataaaa acaggtatta
     4861 acgctgatga taaacttgtt gcactcgcaa agcccaaatg aatcaatata ttaaaaagcg
     4921 tgataagtgc aattgtcgcg ataataggta aatgttttaa acctaaaaca ccaaaagttt
     4981 gatcacttaa ccattgagct gcacctgttt tcaaaagaac gttacctagt gaaataccta
     5041 caccaaacac gataattgtt ccccatggta ttttattttc aacatgtttc catgtcatga
     5101 caccaatttt cggcattaac ataacaccta aagcaataat agtaatggat gcagagtcaa
     5161 tcggatgtaa tactttttca gttgaccaaa acagtaataa taacatcgat ataacaatta
     5221 aacgccattc acgtgggcta acggggccaa gtttatgcaa ttcttctttt attaaatctt
     5281 taccaccttc tattgtatta atttctggag gcatcacttt aatcatgatg aaatataaag
     5341 ctacggacat aactatggac caaggcgctg catataagaa ccactcgccc catgaaacat
     5401 caaatcctaa ttgatggttt ataaaattaa tcgctacgat attttgtgct gccgccgttt
     5461 tgataccaat attccaaatt gacacagctt gtactgaagt aattattaat aaagacgcta
     5521 acttgctatc tttggaaact ttaaatgccg caatcatacc cagcaagatt ggtacaactg
     5581 cccctgctct agctgttgca gaaggaacga aaaatgcaag tacaattgaa acgataattg
     5641 ctccaataac tatatttcta gttttattac caacaattga taacactaaa agagctagtc
     5701 ttttatgcaa attcgtttct tgcatagcag cagccaaaaa taatgcagct gcaacgagag
     5761 ctacagctga agttgcaaat ccactaaacg ctaatgataa tgcatgatta gttcctagaa
     5821 ggtcacttcc agctaaaata gcactgccac ttttcggatt acctagcttc tcccctaaat
     5881 tttgaacagg gctaaatcct aaaagtaata tcattaagcc aataattaaa gttgctgaca
     5941 ccggatatga tacagcttcc gttacccaca taataactgc aaaagctaaa atagctagta
     6001 ctgccttagc cattatcggc agacttgaag gtgccgggag taatagtaca actataagta
     6061 ttataaaact catgacaatc caaaatggct tgtattgctt ttttgtcttt tctttctttt
     6121 gttgttcatg ttcaactaca tgtttttctt ctgacataac agtcacccct ttgtttaatc
     6181 ttaatttaat tataacaatt ttttaaaaca tacattttta atttatgcaa aatacacaat
     6241 tttatcgaaa tattttgtaa ataatattgc taggtgatga gttatcttgg ttaattacaa
     6301 aacgatatct aagtttaaat attagatcat aaaaaaacta tctactgaat atcctcatag
     6361 catcattttt ctaagcaaaa gttaaactta gttagataaa atgcaaatga gattattgta
     6421 gatagtctct ttttaatgtt taaaaatgat taatgccata aacataaata attccaaaga
     6481 atgccgcgcc actaagcact gttaatataa gacttagtaa caatgtgcgt ttataatgtt
     6541 taacaaatgc tatgaacaaa atgactacat tatacgcaaa gaaaagtccg aataatgtca
     6601 ttgttatttt caaatcagaa tagaaaatat ttaatagtaa tacaatgact gcataaatta
     6661 aatacggaat aactataaat ttgcgttcaa atcttaattg ttcgaaacgt ttatcttcgt
     6721 ttgtcatgat atgctccttt atgatttaaa agtaaataag tttacggaat atcttgtccg
     6781 ataatagcgg tgtaaatgtc aaaccacgac tgatcatcta aattaagttg tagcccttcg
     6841 attgcttgat caatacgctt taactgactt gttccaagta tcggcatgat acgatgcggt
     6901 atttttacta accacgctat catcacagct gtgtcactca caccatattt gtcagctatt
     6961 gattgaacaa ctttcataat acgttgcgct ttaatatctt ccttgtcgaa aattttaccg
     7021 cctgcaaaag gactccaagc catgatttga acatggtttt gatacattga atccattgtt
     7081 ccatcttgta aactatcaac gtgatatggc gataattcta attgattgat gctaatatgt
     7141 agtctttctt tcataatata ttgatttaac aattggtatt gtgaatgatt aaaattcgac
     7201 accccgaatg acttcaactt accttgttta acaagtttag ttaatgcatc agcaacttgt
     7261 tctggatcca tcaatggtga aggacgatga atgagtagac tatctaaata atctacattc
     7321 aaattgatta atgactgttc aacagatttc acgatgtgct tactactcaa atcataacga
     7381 tgtccatttg taaaatcaaa ttgcttagaa ggcaaaatga taccacattt cgtaacaatt
     7441 tgaattttat ttcttaattc gggtgataaa tccaaagcat taccaaacag tgattcacat
     7501 tgataatctc cataaatatc agcatgatcc atcgttgtaa ttccacgttc aactaattca
     7561 tttataaaat aatttaactc tttcgcagtc atcttccatt catttgcacg ccaaaaacct
     7621 tgtacaagcc tagaaaaatg aacatagtga ttaatcatta tttgttccat atctcatcca
     7681 ctccatccct caatgtcaca aaatattcac aataaaagta ttaagaaatt aatgtgctat
     7741 ttccgttaaa ttgaaacaac acattttctg atttacgtaa attgtagcat atccaattga
     7801 ttacgtatgt gatttgtatc taaagtttca ccaataataa caatcgttaa tggtaattgc
     7861 atgccaatta ttccatagtc tggtaaacca tatgcatatt gaaattcata aattgcattt
     7921 ggttgatctc taaatgacac ataacctttc aaacgtagaa cagattccgg taatttcatt
     7981 ataaattgat aaaacaaatg acgatcaatc ggacctgtaa acgtgtaagt catactttta
     8041 atcccatgat gatggtgatg atgagacgat atctctctgt ctttaacagt taagtccaat
     8101 tcttccaacg aaacttttcc ataggttgtt ttataagttg gaatacttgc acaaatcata
     8161 cctaattgct tatcaatttt ctcaagactg tcatcagtta taagatcaat tttattaata
     8221 ataatcatgt cacttagttt caactgatct tccatcagcg aaactgtatt ttcggtatat
     8281 tgatgacgtt ctaaaaatcg agtcgcatct aatacaccat aaataatcgg cttttcaaag
     8341 aaattaacga tttgcggatc ttgacatgca actagtaatt ccaaaggatg cgcaatgcct
     8401 gtcgcttcta tgatgacatg attaacgtcc ccttttaaag caatggcttt tagttcatag
     8461 acaagttctt gtttaagatc gcaacaaaca caaccattaa tcaatgaatg gacttcaatt
     8521 tcatttgaaa tactattgct atcaatatca aaagtaccga attcattcat gatgatttta
     8581 attttctcat cattttttaa taattcacta atataatgtg tcagtaacgt ggttttacca
     8641 ctacccaaaa atccgttaat tatgga
//
LOCUS       DAXWFK010000108         8451 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.108, whole genome shotgun sequence.
ACCESSION   DAXWFK010000108 DAXWFK010000000
VERSION     DAXWFK010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8451
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.108"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            274..960
                     /gene="mtnN"
                     /locus_tag="ACGEXS_001888"
     CDS             274..960
                     /gene="mtnN"
                     /locus_tag="ACGEXS_001888"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFF8012887.1"
                     /translation="MIGIIGAMEEEVTILKNKLTQLSEISVAHVKFYTGILKDREVVI
                     TQSGIGKVNAAISTTLLINKFKPDVIINTGSAGALDESLNVGDVLISDDVKYHDADAT
                     AFGYEYGQIPQMPVAFQSSKPLIEKVSQVVQQQQLTAKVGLIVSGDSFIGSVEQRQKI
                     KKAFPNAMAVEMEATAIAQTCYQFNVPFVVVRAVSDLANGEAEMSFEAFLEKAAVSSS
                     QTVEALVSQL"
     gene            980..1507
                     /locus_tag="ACGEXS_001889"
     CDS             980..1507
                     /locus_tag="ACGEXS_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFF8012888.1"
                     /translation="MGLVRKFFMPNSYVQSIFQIDLDKLVDKGVKGIITDLDNTLVGW
                     DVKEPTERVKAWFKEANEKGITITIVSNNNESRVASFSQHLDIDFIFKARKPMGKAFD
                     KAITKMNIRPDQTVVIGDQMLTDVFGGNRRGLYTIMVVPVKRTDGFITKFNRLIERRL
                     LRHFSKKGYITWEEN"
     gene            1508..2608
                     /gene="yqeH"
                     /locus_tag="ACGEXS_001890"
     CDS             1508..2608
                     /gene="yqeH"
                     /locus_tag="ACGEXS_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFF8012889.1"
                     /translation="MSDILKCIGCGAPLQSEDKNKPGFVPEHNMFRDDVICRRCFRLK
                     NYNEVQDVGLESEDFLKLLSGLADKKGIVVNVVDVFDFEGSFINAVKRIVGNKKIILA
                     ANKLDLLPKQINKRRVKEWLKRTARKYGLEADDVVLISAEKGWGIDDLLSSIANIREN
                     EDVYIVGTTNVGKSTLINKLIEASVGEKDVVTTSRFPGTTLDMIDIPLDETSFMYDTP
                     GIIQDHQMTHLVSEKELKIIMPKKEIKQRVYQLNEAQTLFFGGLARIDYVSGGKRPLV
                     CFFSNDLNIHRTKTEKANDLWRNQLGDLLTPPGNPQNFDLNEVKAVRLETGKEKRDVM
                     ISGLGFITIGPGAKVIVRVPKNVDVVLRNSIL"
     gene            2622..3428
                     /gene="aroE"
                     /locus_tag="ACGEXS_001891"
     CDS             2622..3428
                     /gene="aroE"
                     /locus_tag="ACGEXS_001891"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFF8012890.1"
                     /translation="MKFAVIGNPISHSLSPVMHRANFNSLGLDDTYEALNIPIEDFHL
                     IKEIISKKELEGFNITIPHKERIIPYLDYVDEQAINAGAVNTVLIKDGKWIGYNTDGI
                     GYVKGLHSVYPDLENAYILILGAGGASKGIAYELAKFVKPKLTVANRTMARFESWNLN
                     INQISLADAEKYLAEFDIVINTTPAGMAGNNESIINLKHLSPNTLMSDIVYIPYKTPI
                     LEEAERKGNHIYNGLDMFVYQGAESFKIWTNKDADINSMKTAVLQQLKGE"
     gene            3432..3722
                     /gene="yhbY"
                     /locus_tag="ACGEXS_001892"
     CDS             3432..3722
                     /gene="yhbY"
                     /locus_tag="ACGEXS_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFF8012891.1"
                     /translation="MLTGKQKRYLRSLAHNIDPIFQIGKGGINENMIKQIDDTLENRE
                     LIKVHVLQNNFDDKKELAETLSEATHSELVQVIGSMIVIYRESKDNKEIELP"
     gene            3725..4294
                     /gene="nadD"
                     /locus_tag="ACGEXS_001893"
     CDS             3725..4294
                     /gene="nadD"
                     /locus_tag="ACGEXS_001893"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HFF8012892.1"
                     /translation="MKKIVLYGGQFNPIHTAHMIVASEVFHELQPDEFYFLPSFMSPL
                     KKHHDFIDVQHRLTMIQMIIDELGFGDICDDEIKRGGQSYTYDTIKAFKEQHKDSELY
                     FVIGTDQYNQLEKWYQIEYLKEMVTFVVVNRDKNSQNVENAMIAIQIPRVDISSTMIR
                     QRVSEGKSIQVLVPKSVENYIKGEGLYEH"
     gene            4284..4868
                     /gene="yqeK"
                     /locus_tag="ACGEXS_001894"
     CDS             4284..4868
                     /gene="yqeK"
                     /locus_tag="ACGEXS_001894"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFF8012893.1"
                     /translation="MNIEKAKRLAKEKLPEKRYNHSLRVAETAIKLAEIYDGDTSKVE
                     LAGVLHDFCKYDDLGKMYQIVRQYELGNDLLSYGSEILHSPVCAAIMEHEYGINDEEV
                     LMAIKYHTTGRQQMTKTEKLIFIADYIEPGRTIPGVDDIRDMAYNQGSLDKTIYEISK
                     RTVLFLIQKDITVYNKTIDCLNYYNYSDERIKDD"
     gene            4869..5222
                     /gene="rsfS"
                     /locus_tag="ACGEXS_001895"
     CDS             4869..5222
                     /gene="rsfS"
                     /locus_tag="ACGEXS_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFF8012894.1"
                     /translation="MNSQELLAIAVDAIDNKKGEDTISLEMKGISDMTDYFVVTHGNN
                     ERQVQAIARAVKEVANEQNIEVKRMEGYNEARWILIDLADVVVHVFHKDERNYYNIEK
                     LYQDAPLESYSQVAY"
     gene            5225..5941
                     /locus_tag="ACGEXS_001896"
     CDS             5225..5941
                     /locus_tag="ACGEXS_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8012895.1"
                     /translation="MSQYAEMSLVYDQLTQDQPYEKWFEIVKNHCKDESNILDIGCGT
                     GSLTVQLEALGNVTGMDLSVDMLTVAAQKSANVNWLEGDMTSFDLQQQFDIITIFCDS
                     LNYLQDETAVIETFINVYHHLTDSGVFIFDVHTVYKMMTLFNNQSYIDDKGDIFLAWD
                     AVQGDLPLSVYHDMTFFIRHEDETYSRFDESHFQRTFDEKTYLSWLAQVGFKHVETFT
                     DFNIDEHNEDAERLFFIAKK"
     gene            5981..6667
                     /locus_tag="ACGEXS_001897"
     CDS             5981..6667
                     /locus_tag="ACGEXS_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFF8012896.1"
                     /translation="MVLLYQFLLRYKDFLTQWKLYILSAVVLIMVLIGFIFWRQDDYT
                     SRNFENKDTALKQSTSENSSLSKVEDVQVKDGDNSKNKGPVYVDVKGAVKHPNVYKMT
                     SKDRVVDLLDKAQLLEDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQIEVNSVHEKN
                     GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKT
                     FDKLKSYFTT"
     gene            6759..7220
                     /locus_tag="ACGEXS_001898"
     CDS             6759..7220
                     /locus_tag="ACGEXS_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFF8012897.1"
                     /translation="MERIKWEEYFMAQSHLLALRSTCQRLSVGATIVKDNRIIAGGYN
                     GSVAGEVHCIDEGCLIEDGHCIRTIHAEMNALLQCAKQGVSTEGATIYVTHFPCLNCT
                     KSIIQAGIKRIYYAEDYHNHEYATKLLKQSGIEFKKIPFSPEYVAKYLTKG"
     gene            7225..>8451
                     /locus_tag="ACGEXS_001899"
     CDS             7225..>8451
                     /locus_tag="ACGEXS_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HFF8012898.1"
                     /translation="MLFVALSMIVGVLWNSSKVLSTFLFILLLYITYRKNKIVYAPIS
                     LFLIIFSAWYLHYSQQAIFNYINYIERNSQFNERAQVIQIQRQGSDTYKGRLSLKNEI
                     YPFFLTDKKNFDLKKIESRNCIVKGQFKVNDNKFVTLKLQSIVVQSCLESNRSNLIEK
                     HKQFIMNRIYDSGIKFPDRIMALITGDVKEVNEQFKERVKEIGIYHLLAVSGSHIAAI
                     VFLIYQPLKRLNLPLFVIKGITIIVLALFAQYTNYAPSAVRAIIMTTLVLVITKQIKI
                     KGIQLLAFAFIIMFILNPLVVYDIGFQFSFIISFFIMLLFPFLQQLSKLQSLFIITFI
                     AQLASFIVAIPSFHQLQWVGFLSNLIFVPYYSIILFPLSILFFITSHFIVGLTPLNYL
                     VDLSFNFHDWLLDLFTR"
BASE COUNT         3046 a         1071 c         1579 g         2755 t
ORIGIN      
        1 tcattttcct gttatttaat aaagtttctt gacacgattg gttgatgtaa ttgatatgat
       61 tagtttttga aaaatagaca aattagaagc atcgaattca ctgttatgag ttcaaaaata
      121 attatttatg ataccaagtt attgaaagag ctttttataa aaataaagca aaagttttat
      181 acagtaatga aaatcatgat acttgtcgtt gtagtgatta aaatgtgata aaataacaaa
      241 tgctaggatt ttttaaaaga ggtgaatcaa aagatgattg gtataattgg tgccatggaa
      301 gaagaagtaa caatattaaa aaataaatta acacaattaa gcgaaatttc agttgcacat
      361 gttaaatttt atactggcat tttaaaagat agagaagtag tgattaccca aagtggcatt
      421 ggaaaagtta atgctgcaat ttctacgaca ttattaatta ataagtttaa accggacgtc
      481 attattaata caggttctgc tggagcttta gatgaaagtt taaatgtagg tgacgttctt
      541 ataagtgatg atgtaaaata tcatgatgca gacgcaacag catttggtta tgaatatgga
      601 caaataccac agatgccggt agcatttcaa tcaagtaaac ctttaataga aaaagtatct
      661 caagttgtac aacaacaaca attaacagct aaagtaggct taattgtaag tggtgatagc
      721 tttatcggta gtgttgaaca acgccaaaaa attaaaaaag catttccaaa tgcgatggcg
      781 gttgaaatgg aagcaactgc aattgcacaa acatgttatc aatttaatgt accatttgtt
      841 gtagttcgtg cagtttcaga cttagcaaat ggagaagcgg aaatgagctt cgaagcattt
      901 ttagaaaaag cagctgtatc atcaagtcaa actgttgaag cattagtgtc tcaattataa
      961 aaaggtaaag gtgagttaaa tgggtttagt tcgcaagttt tttatgccga attcatatgt
     1021 tcaatcaata tttcaaattg atttagacaa gttagtggac aaaggcgtta aaggtattat
     1081 tacagattta gataatacgc tagtaggttg ggatgttaaa gaacctacag aacgtgttaa
     1141 agcatggttt aaggaagcta atgaaaaagg aatcactatt acaatcgtgt ctaataataa
     1201 tgagtctcgt gttgctagtt ttagtcagca tttagacatc gattttattt ttaaagcgag
     1261 aaagccaatg gggaaagcgt ttgataaagc aataactaag atgaatatca gaccagatca
     1321 aactgttgtt ataggtgacc aaatgcttac tgatgtattt ggtggtaatc gtcgaggtct
     1381 atatacaatt atggttgttc cagttaaacg aactgatggc tttattacta agtttaatag
     1441 attaattgaa agacgattat tacgtcattt cagtaaaaaa ggttatatca catgggagga
     1501 aaattgattg tctgacattt taaaatgtat cggttgtggt gcgccacttc aatctgaaga
     1561 taaaaataaa cctggttttg taccagagca taatatgttt cgtgatgacg tgatttgcag
     1621 acgttgtttc cgcttgaaaa attataacga agttcaagat gtaggattag aaagtgaaga
     1681 ctttttaaaa ttattatcag gacttgcgga taaaaagggt attgtcgtca atgtcgtgga
     1741 tgtatttgac tttgaaggat catttattaa tgcagttaaa cgtattgtcg gaaacaaaaa
     1801 aatcatttta gcagcaaata aattggattt attgccgaaa caaattaata aacgtcgagt
     1861 taaagaatgg ttaaaacgaa cagcaagaaa atatggtttg gaagctgacg atgtcgtatt
     1921 aatttcagct gaaaaaggct ggggcataga cgacttatta tcatcaattg cgaatattcg
     1981 agaaaatgaa gatgtgtata ttgtagggac aacgaatgtt gggaaatcta cattgattaa
     2041 taaactgatt gaagctagtg ttggtgaaaa agatgtagta acaacttcaa gattccctgg
     2101 aacaacttta gatatgatag atattccttt agatgaaaca tcatttatgt atgatacacc
     2161 aggtattatt caagatcacc aaatgacgca tttagttagt gaaaaagaat tgaaaattat
     2221 tatgcctaag aaagaaataa aacaacgcgt atatcaatta aatgaggcgc agacattatt
     2281 cttcggcggt ctagcgcgca tagattatgt atcaggtggt aaacgtccgt tagtttgttt
     2341 cttttctaat gacttgaata tacatcgtac taaaacggag aaggctaatg atttatggcg
     2401 taatcaactt ggcgatttat taacgccacc tggaaatcca caaaattttg atcttaatga
     2461 ggtaaaggct gttagacttg aaacaggcaa agagaaacgc gatgttatga tctctggtct
     2521 aggctttata actataggac caggggctaa agtaatcgtt cgtgttccta aaaatgttga
     2581 tgttgtatta agaaattcta ttttataagg tgattaaaaa aatgaaattt gcagttatag
     2641 gaaatcctat ttcacattcc ttgtcgcccg ttatgcatag agcaaatttt aattctttag
     2701 gattagatga tacttatgaa gctttaaata ttccaattga agattttcat ttaattaaag
     2761 aaattatttc gaaaaaagaa ttagaaggct ttaatatcac aattcctcat aaagaacgta
     2821 tcataccgta tttagattat gttgatgaac aagcgattaa tgcaggtgca gttaacactg
     2881 ttttgataaa agatggcaag tggatagggt ataatacaga tggtattggt tatgttaaag
     2941 gattgcacag cgtttatcca gatttagaaa atgcatacat tttaattttg ggcgcaggtg
     3001 gtgcaagtaa aggtattgct tatgaattag caaaatttgt aaagcccaaa ttaactgttg
     3061 cgaatagaac gatggctcgt tttgaatctt ggaatttaaa tataaaccaa atttcattag
     3121 cagatgctga aaagtattta gctgaattcg atatcgttat taatacaaca ccagcgggta
     3181 tggctggaaa taacgaaagt attattaatt taaagcatct ttctcccaat actttaatga
     3241 gtgatattgt ttatataccg tataaaacac ctattttaga ggaagcagag cgcaagggaa
     3301 accatattta taatggctta gatatgtttg tttaccaggg tgcggaaagc tttaaaattt
     3361 ggactaataa agatgctgat attaattcta tgaaaacagc agttttacaa caattaaaag
     3421 gagaataaca tatgcttact ggcaaacaaa aaagatactt aagaagttta gcacacaata
     3481 ttgatccgat ttttcaaatt ggaaaaggcg gtatcaacga aaatatgatt aaacaaatag
     3541 atgatacgtt agaaaacaga gaattgatta aagtacatgt actacaaaat aactttgatg
     3601 ataaaaaaga attagctgaa acattaagcg aagctactca tagtgaatta gtgcaagtga
     3661 ttggatctat gatagtgatt tatagagaat ctaaagataa taaagaaatt gaattgccat
     3721 aataatgaaa aagatagtac tttacggcgg tcagtttaac cctatccata ctgcacatat
     3781 gatagtagct agcgaagtat ttcatgaatt acagccagat gaattttatt ttttacctag
     3841 ttttatgtct ccattgaaaa agcaccatga ttttatagac gttcagcaca gattaacaat
     3901 gatacagatg attatcgacg agcttggttt tggagatatt tgtgacgatg aaattaaacg
     3961 tggtggtcaa agttatacct atgacacgat caaggcattc aaggagcaac acaaagacag
     4021 tgagttgtac tttgttattg ggacggatca gtataaccaa ctagagaaat ggtatcaaat
     4081 tgaatactta aaagaaatgg ttacttttgt agttgtaaat cgagacaaaa atagtcaaaa
     4141 tgttgaaaat gctatgattg caattcagat acctagggta gatataagtt cgacaatgat
     4201 tcgacaaaga gttagtgaag ggaaatctat ccaagttctt gttcctaaat ccgttgaaaa
     4261 ctatattaag ggggaaggat tatatgaaca ttgaaaaagc aaaacggctt gcaaaagaga
     4321 aactgccaga gaaacgttat aatcattctt taagagttgc agaaacagcg attaaattgg
     4381 cagaaattta tgatggagac actagtaaag tagaattagc aggtgtatta catgatttct
     4441 gtaaatatga tgatttaggt aaaatgtatc aaattgttcg acaatacgaa ttaggtaatg
     4501 atctactgag ttatggtagt gaaatattgc atagccctgt gtgtgcagca atcatggaac
     4561 atgaatatgg tatcaatgat gaggaagtat taatggctat caaataccat actactggac
     4621 gtcaacaaat gacaaaaact gaaaaactga tttttattgc agattacatc gaacctggaa
     4681 gaacaatccc aggagttgat gatattcgag atatggcata caatcaaggt agtttagata
     4741 agacaattta tgaaatttct aaacgcacag tactattttt aatacagaaa gatattacgg
     4801 tatataataa gacgattgac tgtttaaatt attataacta tagtgacgaa agaataaagg
     4861 atgattaaat gaattcacaa gaattattag caattgctgt ggatgcaatt gacaataaaa
     4921 aaggcgaaga tacgatttct ttagaaatga aaggtatcag cgatatgaca gattattttg
     4981 ttgtaacgca cggaaataat gaacgacaag ttcaagcgat tgctagagcg gtgaaagaag
     5041 tagccaatga acaaaatata gaagtaaaac gtatggaagg atacaatgaa gcgcgttgga
     5101 tattaattga cttagctgat gttgtggtac atgttttcca taaagacgaa agaaattatt
     5161 ataatattga aaagttatat caagatgcac cattagaatc atatagtcag gttgcgtatt
     5221 aattatgtcg caatatgcag aaatgagcct agtgtacgat caattgactc aagatcaacc
     5281 atatgaaaaa tggtttgaaa ttgtaaaaaa tcactgcaaa gatgaatcaa atattttaga
     5341 tattggatgc ggtactggta gtttaacagt tcaattagaa gctttaggta atgttactgg
     5401 tatggattta agtgttgata tgttgactgt tgcagctcaa aaatcagcaa atgtaaattg
     5461 gcttgaagga gatatgacat cttttgattt gcaacaacaa tttgatatca tcactatttt
     5521 ttgtgactct ctaaattatt tgcaagatga aactgcagtg attgaaacat tcatcaatgt
     5581 ttatcatcat ctgactgatt caggtgtatt tatttttgat gtacatactg tttataaaat
     5641 gatgactttg tttaataatc aaagttatat tgatgataaa ggggacattt ttttagcatg
     5701 ggatgctgtg caaggagatt tacctttaag tgtttatcat gatatgacat ttttcattcg
     5761 acatgaagat gaaacatatt caagatttga tgagtctcat tttcagagaa catttgatga
     5821 aaagacatat ttgtcttggt tagctcaagt tggttttaaa catgttgaaa catttactga
     5881 ttttaatata gatgaacata atgaagatgc agaaagattg tttttcattg cgaaaaaata
     5941 aaagtaaaac cactcgctca gacgtgtata aagttgagga gtggttttat tgtatcaatt
     6001 tttattacgc tataaagatt ttttaactca atggaagtta tatattctaa gtgctgttgt
     6061 tttaattatg gtattaattg gttttatatt ctggagacaa gatgattata cttcaagaaa
     6121 ttttgaaaat aaagatactg ctctgaaaca aagcactagt gaaaatagta gtttgtccaa
     6181 agtagaagat gtccaggtca aagatggaga taattccaaa aataagggtc ctgtatatgt
     6241 cgatgtaaaa ggtgctgtta aacatcctaa tgtttataaa atgacatcta aggatagagt
     6301 agttgattta cttgataaag cacaattatt ggaggatgca gatgtaagtc aaattaattt
     6361 gtctgaaaaa ttaacagatc aaaaaatgat tttcatacct cataaaggac aaaagaatgt
     6421 tgaaccacaa attgaagtaa acagtgtgca cgaaaaaaat gggaacacaa ataatactaa
     6481 agtaaattta aatacggcat ctgtatcaga attgatgtct gttcctggag ttgggcaagc
     6541 taaagctaat gcaattgttg aatatcgcaa ccaacaaggt gcatttcaag aaattgacga
     6601 tttgaaaaaa gtaaaaggtt ttggaagtaa aacttttgat aaactgaaat cttatttcac
     6661 gacataatgg attattttaa ttgcaaattt tattttttgt gattaatctt atattattag
     6721 aaaatgttac taaattttaa gtatggaagg tgaacatctt ggaaagaatc aaatgggaag
     6781 aatattttat ggcacaaagt catttgctag cattacgttc aacttgtcaa agattatctg
     6841 taggtgcaac gattgttaag gataatcgta ttattgctgg tggttataat ggctctgtag
     6901 ctggcgaggt gcattgtata gatgaaggat gtttaattga agatggacat tgtatcagaa
     6961 cgatacatgc agaaatgaat gctttattac aatgtgcaaa acaaggtgta tctactgaag
     7021 gtgcaacaat ctatgttact cattttccat gcctaaattg tacaaagtca attattcaag
     7081 caggtataaa gcgtatctac tatgcagaag attatcataa ccatgaatat gcaactaaat
     7141 tactcaaaca atctggtatt gaatttaaaa aaattccatt ttcaccagaa tatgttgcta
     7201 aatatctgac taaaggttaa tgttttgctg tttgtcgcgt tatcaatgat tgtaggagtg
     7261 ctttggaatt ctagcaaagt gctctctaca tttcttttca ttttactttt gtatattact
     7321 tatcgtaaaa ataaaatcgt ttatgcccct atttctctct ttttaatcat tttctccgca
     7381 tggtatttac attattcaca acaagcaata tttaattata tcaattatat tgaacgtaat
     7441 tctcagttta atgagcgtgc tcaagtaatc caaattcaac gtcaaggtag tgacacatat
     7501 aaaggtaggt tgagtttaaa aaatgaaata tatcctttct ttttaacaga taaaaagaat
     7561 tttgatttaa agaaaattga aagtcgtaat tgtattgtta aaggacaatt caaagttaat
     7621 gacaataagt ttgtaactct taaattacaa agtatagttg tacaaagctg cctagaatcg
     7681 aaccggtcta atttaattga gaaacataaa cagtttataa tgaatcgaat ttatgattcg
     7741 ggtattaagt ttccggatcg tattatggca ttgattactg gtgacgtaaa agaagttaat
     7801 gagcaattta aggaacgtgt taaagagata ggtatatatc atttgctggc agttagtggc
     7861 tcgcatatag ctgcaattgt atttttaatt taccaacctt taaaacgatt aaatttacct
     7921 ttatttgtca ttaaaggaat tacaatcatt gtattagctt tatttgctca atacacaaat
     7981 tatgcaccta gtgctgtaag agctataata atgacaactc ttgtactggt tattactaag
     8041 caaattaaaa taaagggtat tcagctatta gcatttgcat ttataattat gtttatttta
     8101 aatccactag ttgtttatga tattggattt caattttcat tcatcatttc attttttatt
     8161 atgctacttt ttcctttttt acagcaattg tcaaagttac aatcattatt cataattacg
     8221 tttattgcac aattagcttc atttatcgtt gccattccaa gctttcatca acttcaatgg
     8281 gtgggatttt tatctaattt gatttttgta ccgtactatt cgattatatt gtttccgcta
     8341 tctattttat tctttattac aagtcatttt attgtgggat taacgccgct aaattacttg
     8401 gttgacctaa gttttaattt tcatgactgg ttactagacc tattcacaag a
//
LOCUS       DAXWFK010000109         8361 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.109, whole genome shotgun sequence.
ACCESSION   DAXWFK010000109 DAXWFK010000000
VERSION     DAXWFK010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8361
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.109"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            436..1308
                     /locus_tag="ACGEXS_001900"
     CDS             436..1308
                     /locus_tag="ACGEXS_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFF8012899.1"
                     /translation="MSNVLTEIDSQYPYMTKNEKKIAKFILNSPQKVIKMRSQDLASL
                     LDISTSSVIRFSKKITDGGFHDLKINISKYVPKASSIYNVELMNNESTESLRTKLHTR
                     TTRALNHANNELNDKTIDQICHCLKRSETIFIYGFGASFVVATDLYQKLSRIGLNIQL
                     VQETHIFATLLATHNSNDSVILITNNGTQSEMQSMVKVIDDYHIPIITITSTRDNPVA
                     QASNIVLTYGKTDENEMHMGATTSLFAQMFTIDILYYRYVALNYHASLDFITQSKMAL
                     DNYRKHLSNINFKH"
     gene            complement(1695..3074)
                     /locus_tag="ACGEXS_001901"
     CDS             complement(1695..3074)
                     /locus_tag="ACGEXS_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFF8012900.1"
                     /translation="MENNELQRGLSARQIQMIALGGTIGVGLFMGATSTIKWTGPSVI
                     LAYLIAGIFLFLIMRAMGEMIYLNPTTGSFATFASDYIHPAAGYMTAWSNIFQWIVVG
                     MSEVIAVGEYMKFWFPELPTWIPGVIAILLLMAANLFSVKAFGEFEFWFALIKVVTII
                     LMIIAGFGLIFFGFGNGGHAVGISNLWTNGGFMPNGIVGFFFALSIVIGSYQGVELIG
                     ITAGETKDPQKNIVKAVNGVIWRILIFYLGAIFVIVSVYPWNQLGDIGSPFVATFAKI
                     GITFAAGLINFVVLTAAMSGCNSGIFSASRMIYTLAHKGEMPKIFTKIMRNGVPLYTV
                     VAVSLGILIGALLNVILPLYIDGAKSIFVYVYSASILPGMIPWFMILFSHLRFRKLHP
                     EELEGHPFKMPGGAVTNYLTILFLILVLVGMVFNVETRISVLIGVIFLTIVTIYYFIR
                     YNKNNVKAK"
     gene            3354..3710
                     /locus_tag="ACGEXS_001902"
     CDS             3354..3710
                     /locus_tag="ACGEXS_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012901.1"
                     /translation="MLKQILLRATKISVLFAVAFFIINYIGMEKPDILYLVGRTIIAT
                     LAFILICLTVFTIINSPERKIKLGTTLPIALIIGIIFGAIFLTVQIGVITGLIIGVIA
                     TFIWELIEKNKGGRSS"
     gene            3707..3892
                     /locus_tag="ACGEXS_001903"
     CDS             3707..3892
                     /locus_tag="ACGEXS_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012902.1"
                     /translation="MTTALIGVIVLILLIISFVPNYRAMKSAKEQGSNATRATIMVGI
                     DIILIVLIIVTLLLKYM"
     gene            complement(3988..4179)
                     /locus_tag="ACGEXS_001904"
     CDS             complement(3988..4179)
                     /locus_tag="ACGEXS_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012903.1"
                     /translation="MMGYIILFFLAGPVILGVGNLVIGPIFNKQTPFRVQVRSFVVGS
                     MIYLILATIGYFLLLQGKL"
     gene            complement(4190..4825)
                     /locus_tag="ACGEXS_001905"
     CDS             complement(4190..4825)
                     /locus_tag="ACGEXS_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFF8012904.1"
                     /translation="MYRAVIFDFDGTIIDTEQHLFNVINKHLEMHNADPISIDFYRSS
                     IGGAATDLHDHLIKAIGSENKDKLYEEHHLTSTTLPMIDTIKSLMAFLKQRHIPMAIA
                     TSSVKAEIMPTFKALGLDDYIEVVVGREDVEQVKPDPELYLSAVQQLNYMPTQCLAIE
                     DSVNGATAAIAAGLDVIVNTNKMTSAQDFSNVDYVAKDIDYDQIVARFFTK"
     gene            complement(4900..5817)
                     /locus_tag="ACGEXS_001906"
     CDS             complement(4900..5817)
                     /locus_tag="ACGEXS_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFF8012905.1"
                     /translation="MFQKLSIFATKSFLVWMLVAAVIGFIFPQHVATLGKWVPYLLGI
                     VMLGMGLTITPNDFKMVFKAPRAVIIGVCLQFSIMPTLAFIIAKSFHLPPDIAVGVIL
                     VGCCPGGTSSNVMSYLAKANVALSVSITTVSTLLAPFVTPALIYLFANEWLEVSFVSM
                     LWSVVQVVLIPIALGIVLQIINRKIAEKASTALPIISVVAISLILAIVVGGSKHQILT
                     TGLLIFLVVILHNVLGYTIGYWLARLLKLDRQDQKAVSIEVGMQNSGLAVSLAALHFN
                     PIAAVPGAVFSFIHNITGPILAKYWSKKL"
     gene            6085..6615
                     /locus_tag="ACGEXS_001907"
     CDS             6085..6615
                     /locus_tag="ACGEXS_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012906.1"
                     /translation="MLIDKFETYIINIAGLNDRTTRKKLSKLCKSVQFCDALQFSINK
                     QFNQYVLEISLPKQQLPYFISFLSFHQYSIFQVLSPKKINELLDSDNLYQSAKRFDIN
                     IDGLQDAFIKDKVIDIMNMFQNHTDITYTLNKSHAHIICTPEIFAKLLHTIATRNIDI
                     LSANYRSSSMSKARIS"
     gene            complement(6681..8285)
                     /locus_tag="ACGEXS_001908"
     CDS             complement(6681..8285)
                     /locus_tag="ACGEXS_001908"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucoside-specific PTS transporter subunit
                     IIBC"
                     /protein_id="HFF8012907.1"
                     /translation="MNAIKRFGSAMIVPVLMFAFFGIVLGFATLFKNPTIMGSLADQH
                     TFWFKFWSVIESGGWVIFTHMEVVFVVGLPLSLAKKAPGHAALAALMGYLMFNTFINA
                     ILTQWPHTFGANLEKGVENVPGLKSIAGIATLDTNILGGIIISAIITWIHNRYYSKRL
                     PEMVGVFQGLTFVVTISFFVMLPLAAITCVIWPTVQHGIGSMQHFIIASGYIGVWLYH
                     FLERVLIPTGLHHFIYAPIEVGPVVVNHGLKAEWLQHLNEFAKSTKPLKEQFPYGFML
                     QGNGKVFGCLGIALAMYATTPKENRKKVAALLIPATLTAVVVGITEPLEFTFLFIAPY
                     LFVLHAVLAASMDTLMYAFGVVGNMGGGLLDFISTNWLPLGKEHWGTYVAQVIIGLIF
                     VAIYFFLFRFLILKFDIPLPGRKKTEEEVKLFSKQDYKNKKGDSVDSKRASSGNEYED
                     KAAYYLDGLGGKENIKDVTNCTTRLRLTVYDESKVADTEYFTHQQMAHGLVKSGKSIQ
                     VVVGMTVPQVREAFEQMVEDQSSEDK"
BASE COUNT         2975 a         1538 c         1144 g         2704 t
ORIGIN      
        1 cataaaatgt catttaaaca agttatttat taaaattcac tttattacat aaattataca
       61 attaaaaagt ttcttcaaat tgtaaagatg cattaatcga gttataatca taatgattaa
      121 gatggtagat ttagtcttat ataactacat ttactaagat ttaaatagat gagtacataa
      181 tgtataaatt gggaatatac agtatgactt acggtaatat atcaatatat tgatatatta
      241 tttgcacatg ggagtatgct attttttatc tatacatctt aaaaaaattt acagttttac
      301 aattcaatat cattaaacaa acaacatatt cagcttattt ttcattctct tacgttacaa
      361 gttgtaaata ccatcgcgtt aaacaacaat agccaacgct aataaaatat attgcgttaa
      421 agtggagtga ttaatatgtc aaacgtacta acagaaatag atagtcaata tccatatatg
      481 actaaaaatg aaaaaaagat agctaaattt atactaaatt cacctcaaaa ggtgattaaa
      541 atgaggtctc aagacttagc aagcttatta gatattagta catcatcagt cattcgattc
      601 agcaagaaaa ttactgatgg cggttttcac gacttgaaaa taaatatatc taaatatgtt
      661 cctaaagcat catcaattta caatgttgaa ttaatgaata acgaaagcac agaatcttta
      721 agaactaaac tccatacacg tactacacgc gcacttaatc atgcaaataa cgaattaaat
      781 gataaaacta ttgatcaaat atgtcattgt ttaaaacgtt ctgagacgat ttttatatat
      841 ggttttggcg catcttttgt agttgctacc gacttatacc aaaagttatc aagaataggt
      901 cttaatattc aacttgttca agaaacacat atttttgcta cattattagc aactcacaat
      961 tcgaacgaca gtgttattct tattactaac aatggtacgc aaagtgaaat gcaatcaatg
     1021 gttaaagtta ttgatgacta ccatatacct ataattacaa ttactagtac aagggataat
     1081 cccgtagcac aggcatcaaa tattgtttta acttatggga aaactgatga aaatgagatg
     1141 catatgggag caacaacttc attatttgct caaatgttca ctattgatat tttatattat
     1201 cgttatgttg cattaaatta tcacgcatca ttagatttca taacacaatc taaaatggct
     1261 ttagataact atcgtaaaca tttatcaaat attaacttta aacattaaca gtcttatata
     1321 aattctttaa tcatgtcata taataattta tgcatcattt tcaaagctta catgtctttt
     1381 atcttgtata acatacgcat atgaccaatg atttgataag gccattcaat ttcttatatt
     1441 aaaaacaaac gcagttgatt aacggattca ttccatatat caattgcgtt tgttatttta
     1501 ataatgctat tttttatttt aaagcccaca gtaaaaacta acgataatat tgccacttat
     1561 aagtttttca cactacaact tcgatttaag ctatatttat aaaaaaatgc gcctactcat
     1621 aacattcttt ggctatttaa ctttaataag ctagggccaa gtttgtcata agataagcgc
     1681 acattataat catactattt tgcttttaca ttatttttat tatatctaat aaaataataa
     1741 atcgttacaa tcgttaagaa gatgacacca ataagcactg atattctagt ttcaacatta
     1801 aataccatac caaccaatac taatattaag aataagattg ttaaatagtt agttactgcg
     1861 ccaccaggca ttttgaatgg gtgaccttcc aattcttcag ggtgtaattt tctaaaacgt
     1921 aaatgactga ataaaatcat aaaccaagga atcatacctg gtaaaataga cgcactataa
     1981 acgtatacga aaatactctt cgctccatcg atatataaag gtaaaattac gtttaataaa
     2041 gcaccaatta aaataccaag agatactgct acaacagtgt acaatggtac accatttctc
     2101 atgattttag taaatatttt cggcatttca cctttatgag caagtgtata aatcatacga
     2161 ctcgcactga aaatacctga gttacatcct gacattgctg cagttaatac aacaaagtta
     2221 attaatccag ctgcaaatgt aataccgatt ttagcaaatg ttgcaacaaa cggacttcca
     2281 atgtctccta attgattcca aggatacact gaaacaataa caaaaatagc gcctaaatag
     2341 aaaattaaaa ttctccagat aacaccattc actgctttaa cgatattttt ctgaggatct
     2401 ttagtttcac ctgccgtaat acctatcagt tccacacctt ggtatgaacc aattacaatt
     2461 gataatgcaa agaagaaacc aacaatacca tttggcataa atccgccatt tgtccataga
     2521 ttagaaatac ctaccgcatg gccaccattt ccaaaaccaa agaaaataag accaaaacca
     2581 gcaataatca ttaaaataat tgttacaact ttaattaaag caaaccaaaa ttcaaattct
     2641 ccaaacgctt ttaccgagaa taaattcgct gccattaata ataaaatagc aataacacca
     2701 ggaatccaag ttggcaattc cgggaaccaa aacttcatat attctcctac tgcgatgacc
     2761 tcactcatac caactacaat ccattggaat atattactcc atgctgtcat ataacctgct
     2821 gcaggatgta tataatcact tgcaaatgtt gcaaatgatc ctgttgtagg gtttaaataa
     2881 atcatttccc ccattgctct catgattaaa aataaaaaga tacccgcaat taaatatgca
     2941 aggataactg atgggcctgt ccatttaatt gtacttgtcg cacccatgaa aagccccacg
     3001 ccaatcgtac caccaagtgc aatcatttga atttgacggg cactcaatcc cctttgtagt
     3061 tcattgttct ccataacttc ttaccccatc tctgattgat actaaataaa tccataaaaa
     3121 aacttattgc cgtaattata ctataaatta tcgattttgg tacattaata tttgcatacc
     3181 atgcaatcgt ttacagaact actatagcag gcttttgcaa ctttgcaaac aattattttg
     3241 tgatttttct aaaaaattaa aaaagttgct caagtaccgt tgagtcacat acaacttcag
     3301 ctattcttgg taattatgat ataattttaa taatattaaa aaggagtata tcaatgttaa
     3361 aacaaatact gctacgtgct acaaaaatat cggtactctt tgctgtagca tttttcatca
     3421 taaattatat cggtatggaa aaacctgaca tcctatattt agttggaaga actattattg
     3481 caacacttgc tttcatactc atttgtctta cagtatttac tattattaat tctccagagc
     3541 gcaagattaa actcggtacg actttaccga ttgcattaat tattggtatc attttcggcg
     3601 ccatattttt aacggtacaa atcggtgtca tcactgggtt aatcatcggt gtgattgcta
     3661 cgtttatttg ggaattgatt gaaaaaaata aaggaggtcg ttcatcatga caacagcatt
     3721 aattggtgta attgtattaa tcttactaat tattagcttt gtgccaaact accgtgctat
     3781 gaaatcagca aaagaacaag gaagtaatgc aacaagagct acaattatgg taggcataga
     3841 tattatctta attgtactta tcatcgtgac gttattatta aaatatatgt aactatttaa
     3901 accaccctat ctcatacata aacatgaacg ttctaccatt tcacatacat atggtcgtcc
     3961 atttaatgag gtaaggtggt tttctcgtta aagtttacct tgtaatagta aaaaatagcc
     4021 aattgttgcg agtattaagt aaatcattga accaacaaca aaagatctta cttgcacgcg
     4081 aaatggtgtc tgtttgttaa atataggacc aatcaccaaa tttccaacgc ctaaaattac
     4141 tggaccagct agaaaaaaca atataatgta acccatcatg atacgcctcc tatttcgtaa
     4201 agaaacgcgc tacaatttga tcgtaatcaa tatcttttgc tacataatct acattagaaa
     4261 agtcctgtgc gcttgtcatt ttattcgtat taacaataac atctaatcca gctgcaatcg
     4321 cggctgttgc accatttaca gaatcttcaa tagccaaaca ttgtgtcggc atataattta
     4381 attgttgtac tgcagataaa tataattcag ggtcaggttt aacttgttca acatcttctc
     4441 taccaacaac tacctctata taatcgtcta gacctaatgc tttaaaggtg ggcattattt
     4501 ccgctttcac actacttgtg gcaattgcca taggaatgtg acgttgcttt aaaaatgcca
     4561 tcaatgattt aatcgtatca atcatcggta atgttgtact agtaagatga tgttcttcat
     4621 aaagtttatc tttattttcc gaaccaatcg ctttaattaa atggtcatgc aaatctgtag
     4681 ctgctcctcc aatagaagaa cgataaaaat caatgcttat aggatcggca ttatgcatct
     4741 ctaaatgttt attaataaca ttaaataaat gttgttccgt atctattatt gttccatcga
     4801 aatcaaatat aactgctcta tacatatatt tactcctcta ttttttctct ttatcaaagc
     4861 ttaacatagt ccgatgacca cttcattcta ttagtgcaat tataactttt ttgaccaata
     4921 ctttgctaaa ataggccctg ttatattatg aatgaaacta aacactgcgc ctggtactgc
     4981 tgcaattgga ttaaaatgca atgctgctaa tgacacagct aaaccagagt tctgcattcc
     5041 aacttcaata ctgactgctt tttgatcttg tcgatctaat tttaaaagac gagctaacca
     5101 atatccaatc gtatacccta atacgttatg taaaataact actaaaaata ttaataatcc
     5161 tgtagttaag atttggtgct tactgccacc tacaactatt gctaaaatta atgaaatagc
     5221 aacaactgat ataattggca aagctgtaga agctttttca gcaattttac gattaataat
     5281 ttgcaaaaca atacctaaag caattggaat taatacaact tgaacaactg accacaacat
     5341 actcacgaaa gatacttcca accattcatt tgcaaataga tatattaacg caggtgtaac
     5401 gaatggcgct agcaacgtag agaccgtcgt aatagaaaca gaaagtgcta cgttagcttt
     5461 ggctaaataa ctcattacat tacttgatgt cccacccgga caacatccaa ctaatattac
     5521 gccaacagca atatcaggtg gtaaatgaaa agactttgca attataaatg ctaatgtggg
     5581 cataatactg aattgtagac agacaccaat aattactgct ctaggtgctt taaagaccat
     5641 tttgaaatca ttaggtgtaa ttgttaatcc catacctaac ataacaatac caagtaaata
     5701 aggtacccat ttacctaatg tcgcaacatg ttgtgggaaa ataaatccaa taacagctgc
     5761 tactaacatc cataccaaaa aactcttcgt ggcaaatata ctcaattttt gaaacattgt
     5821 taacacatcc tcgtcgataa tattgaaaaa tattaccata agaccagtaa ggttacaatt
     5881 acttttctga aaattaagat tatttagtca ttctcttctt attgcaaata ttttatcact
     5941 gataaacaat tattttaaac ttcattcaaa agtcttagtg aataaagaaa aaaagcgtgg
     6001 taaaatcaac ttgaacaaaa ttaagtcacc tttataaata ttaaacgtgt atttaatgcc
     6061 atgtattcct cgggaggtcc tcacatgtta attgataaat tcgaaacata catcatcaac
     6121 atcgccggtt taaatgaccg tactactaga aaaaaattaa gtaagttatg caaaagtgta
     6181 caattttgtg atgcgttgca attctcaata aacaaacagt ttaatcagta tgtattagaa
     6241 attagcttac caaagcaaca acttccatac ttcatttcat ttttaagttt tcatcagtat
     6301 agtattttcc aagtactttc accaaagaaa ataaatgaat tacttgattc tgataattta
     6361 taccaatctg caaagcgttt cgatatcaat attgatggct tgcaagatgc tttcattaaa
     6421 gacaaagtca tcgatattat gaatatgttc caaaatcaca ctgatatcac ttatacgttg
     6481 aataagtctc atgcacatat tatatgtacg ccagaaatat ttgctaaatt gttacatacg
     6541 attgcaacta gaaatatcga cattctatct gccaattata gatcgtcatc tatgagcaaa
     6601 gctcgtatat cataataaaa gacacatcta tacgatgatc atttacaatg attgcgtata
     6661 gtgatgtgtc tttttttgta ttatttatct tcggacgatt gatcctcaac catttgttca
     6721 aatgcttcac gaacttgtgg tactgtcata cctactacaa cttggatact cttaccactc
     6781 tttactaatc cgtgtgccat ttgttgatgc gtgaaatatt ccgtatctgc aactttactt
     6841 tcatcataaa cagttaagcg taatcttgtt gtacagttag taacatcttt gatattttct
     6901 ttgccgccta aaccgtcgag gtaatatgca gctttgtctt cgtattcatt accagatgaa
     6961 gcacgtttag aatctacgct atcacctttt ttatttttgt aatcttgctt agaaaataat
     7021 ttaacttcct cttctgtttt cttacgtcca ggtaatggaa tatcaaattt caaaattaag
     7081 aatctgaata agaagaagta aatcgctaca aatatcagac cgataataac ttgtgcaaca
     7141 taagtacccc aatgttcttt acctaatggc aaccagtttg ttgaaataaa gtctaacaag
     7201 ccaccaccca tgttacctac aacaccaaat gcgtacatta aagtatccat tgatgctgct
     7261 aaaactgcgt gtaaaacaaa taagtatggt gcgatgaata agaatgtaaa ctctaatggc
     7321 tcagtaatac ctacaacgac tgctgttaat gtggcaggaa taagtagtgc cgccaccttt
     7381 ttacgatttt ctttaggtgt cgtagcatac attgctaaag caatacctaa acatccaaat
     7441 actttcccat ttccttgaag cataaagcca taagggaatt gctcttttaa tggcttagta
     7501 ctcttagcaa actcatttaa atgttgtaac cattcagctt tcaagccgtg attgactact
     7561 actggtccaa cttcaattgg tgcataaata aagtgatgta atcctgttgg tattaataca
     7621 cgttctaaga agtgatacaa ccatacaccg atatagcctg aagcaatgat aaagtgttgc
     7681 attgaaccaa taccgtgctg aacagttggc caaataacac aagtaattgc tgctaatggt
     7741 aacatcacaa aaaatgaaat cgtaactaca aatgttaaac cttggaatac accaaccatt
     7801 tctggtaatc gtttactata gtatctattg tgaatccaag taataatagc tgaaataata
     7861 ataccaccta gaatattcgt atccaatgtg gcaatacctg caattgattt taaaccaggt
     7921 acattttcaa cgcctttttc taaattagcg ccaaacgtat gtggccattg tgttaaaatg
     7981 gcatttataa atgtattaaa cattaagtaa cccatcaatg ctgcaagtgc tgcatgacct
     8041 ggtgcttttt tagctaaaga aagtggtaga cctacaacaa atacaacttc catatgcgta
     8101 aaaataaccc agccaccaga ttctattact gaccaaaatt taaaccaaaa cgtatgttga
     8161 tctgctaaac tccccatgat tgtaggattt ttaaataatg tcgcaaagcc aagcactatc
     8221 ccaaagaaag cgaacattaa taccggtaca atcattgcac taccgaaacg ctttatcgca
     8281 ttcatcttct attccctcca tatcatcttt cctaacaata catctaatta gattcattta
     8341 taaatagatg tcttactatt t
//
LOCUS       DAXWFK010000110         8207 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.110, whole genome shotgun sequence.
ACCESSION   DAXWFK010000110 DAXWFK010000000
VERSION     DAXWFK010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8207
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.110"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(105..1115)
                     /locus_tag="ACGEXS_001909"
     CDS             complement(105..1115)
                     /locus_tag="ACGEXS_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFF8012908.1"
                     /translation="MTSLKERDYFFDNARAILILLVVFGHMLQPYTSGDKYLSALYLV
                     IYSFHMPTFLFISGYFAKNIDKPYYLEKISKRLIVPYMIFFAFFSIYYFLTGKSDELQ
                     LDPFNPVFALWFLITLFFFHVILVIVRRFNPYKVLSVSIIISIGAGFSDNIDSYLSIS
                     RTIVFFPIFYLGYIFTKKHTAIFKNKKLIPVSIITFILFFIVYVIHPINADWLLGSSP
                     YTSLENEGQSIFSPFKRLILYGIILIAMTAFLNLMSTKKKLYTYIGSRTLYVYLLHGL
                     IIGIVRGFEWYPFDNPISLMTYLYLISISVIIVYVLSTNFVCKWTNPIINLQRPSQFK
                     DS"
     gene            1268..1687
                     /locus_tag="ACGEXS_001910"
     CDS             1268..1687
                     /locus_tag="ACGEXS_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFF8012909.1"
                     /translation="MYKQLEKLITLTNNDLNLVNRRFGQRTDITSEQLELLRILFNYD
                     RLSQYDLTMKISREQSIVSRWIKKLVLKGYITSQQSSEDLRCKELILTDQARTLISQI
                     NNARCELIEARCQCLSEVELDNLNQLLDKLNQRRISL"
     gene            1714..1824
                     /locus_tag="ACGEXS_001911"
     CDS             1714..1824
                     /locus_tag="ACGEXS_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012910.1"
                     /translation="MSLCTQQKLNHELQAKVAVIVKIDGKQSPLFLLVYV"
     gene            complement(1894..5664)
                     /gene="atl"
                     /locus_tag="ACGEXS_001912"
     CDS             complement(1894..5664)
                     /gene="atl"
                     /locus_tag="ACGEXS_001912"
                     /EC_number="3.2.1.96"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional autolysin"
                     /protein_id="HFF8012911.1"
                     /translation="MAKKFNYKLPSMVALTLVGSAVTAHQVQAAETTQDQTTNKNVLD
                     SNKVKATTEQAKAEVKNPTQNISGTQVYQDPAIVQPKTANNKTGNAQVSQKVDTAQVN
                     GDTRANQSATTNNTQPVAKSTSTTAPKTNTNVTNAGYSLVDDEDDNSENQINPELIKS
                     AAKPAALETQYKTAAPKAATTSAPKAKTEATPKVTTFSASAQPRSVAATPKTSLPKYK
                     PQVNSSINDYIRKNNLKAPKIEEDYTSYFPKYAYRNGVGRPEGIVVHDTANDRSTING
                     EISYMKNNYQNAFVHAFVDGDRIIETAPTDYLSWGVGAVGNPRFINVEIVHTHDYASF
                     ARSMNNYADYAATQLQYYGLKPDSAEYDGNGTVWTHYAVSKYLGGTDHADPHGYLRSH
                     NYSYDQLYDLINEKYLIKMGKVAPWGTQSTTTPTTPSKPTTPSKPSTGKLTVAANNGV
                     AQIKPTNSGLYTTVYDKTGKATNEVQKTFAVSKTATLGNQKFYLVQDYNSGNKFGWVK
                     EGDVVYNTAKSPVNVNQSYSIKPGTKLYTVPWGTSKQVAGSVSGSGNQTFKASKQQQI
                     DKSIYLYGSVNGKSGWVSKAYLVDTAKPTPTPTPKPSTPTTNNKLTVSSLNGVAQINA
                     KNNGLFTTVYDKTGKPTKEVQKTFAVTKEASLGGNKFYLVKDYNSPTLIGWVKQGDVI
                     YNNAKSPVNVMQTYTVKPGTKLYSVPWGTYKQEAGAVSGTGNQTFKATKQQQIDKSIY
                     LFGTVNGKSGWVSKAYLAVPAAPKKAVAQPKTAVKAYTVTKPQTTQTVSKIAQVKPNN
                     TGIRASVYEKTAKNGAKYADRTFYVTKERAHGNETYVLLNNTSHNIPLGWFNVKDLNV
                     QNLGKEVKTTQKYTVNKSNNGLSMVPWGTKNQVILTGNNIAQGTFNATKQVSVGKDVY
                     LYGTINNRTGWVNAKDLTAPTAVKPTTSAAKDYNYTYVIKNGNGYYYVTPNSDTAKYS
                     LKAFNEQPFAVVKEQVINGQTWYYGKLSNGKLAWIKSTDLAKELIKYNQTGMTLNQVA
                     QIQAGLQYKPQVQRVPGKWTDANFNDVKHAMDTKRLAQDPALKYQFLRLDQPQNISID
                     KINQFLKGKGVLENQGAAFNKAAQMYGINEVYLISHALLETGNGTSQLAKGADVVNNK
                     VVTNSNTKYHNVFGIAAYDNDPLREGIKYAKQAGWDTVSKAIVGGAKFIGNSYVKAGQ
                     NTLYKMRWNPAHPGTHQYATDVDWANINAKIIKGYYDKIGEVGKYFDIPQYK"
     gene            complement(5892..6326)
                     /locus_tag="ACGEXS_001913"
     CDS             complement(5892..6326)
                     /locus_tag="ACGEXS_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8012912.1"
                     /translation="MFSKVNNQKMLEDCFYIRKKVFVEEQGVPEESEIDEYESESIHL
                     IGYDNGQPVATARIRPINETTVKIERVAVMKSHRGQGMGRMLMQAVESLAKDEGFYVA
                     TMNAQCHAIPFYESLNFKMRGNIFLEEGIEHIEMTKKLTSLN"
     gene            complement(6481..6951)
                     /locus_tag="ACGEXS_001914"
     CDS             complement(6481..6951)
                     /locus_tag="ACGEXS_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499548.1"
                     /note="Essential protein for resistance to osmotic stress
                     from salt; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic stress response protein"
                     /protein_id="HFF8012913.1"
                     /translation="MSRKTYEKIANINGMFNMLEQQIIHSQDMAHFRSEFFYVNHEHR
                     ENYEALLIYYKNSIDNPIVDGACYILALPEIFNSVDVFESELPFSWVYDENGITETMK
                     SLSIPLQYLVAAALEVTDVNIFKPSGFTMGMNNWNIAQMRIFWQYTAIIRKEAL"
     gene            complement(6999..>8207)
                     /locus_tag="ACGEXS_001915"
     CDS             complement(6999..>8207)
                     /locus_tag="ACGEXS_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase"
                     /protein_id="HFF8012914.1"
                     /translation="FLKYFLILLALVLIVVPIVFATLLFKTSQDAFESSQDSKNANRQ
                     SNLRDNKVNPEEQPISILFLGIDDNDGRRKKGQDAEHSRSDAMILTTFNQSKHQIRML
                     SIPRDTISYIPKVGYYDKITHAHAYGGPIAAMDSVEATMNVPVDYYVRVNMKAFVEAV
                     NELGGIYYDVPYDLNEPNTDDTGKIKIKKGYQKLNGDEALAVARTRHHDSDLKRGQRQ
                     MELIKILFQKAQEVDSIDKLDNVIQIVGKNAKHNLTNSEIKALAKMYLTNDVEIKTAQ
                     LKGKDDMLNGIYYYHPSVESIQKYANLLRKDLELSPINDKNDFLDQRVINHYGSLIPL
                     TPLDNSLLRKEQNDTTDKDKTSNENSDSTNNSDSSNQQQPATDQNSNQNQGGTQQAPQ
                     ASNNQNGVVN"
BASE COUNT         2521 a         1297 c         1350 g         3039 t
ORIGIN      
        1 acttaagctt tatttgaaaa acgaaaaatg tctaaataca ctgtagtaac tacttttcaa
       61 aatgaataaa gtggttacag ttaatgtact tagacagtat aaaattatga atctttaaac
      121 tgtgatggtc tttgtaaatt aattattgga tttgtccatt tacaaacaaa gtttgtagat
      181 aatacatata cgatgattac agatatactt attaaataaa gataagtcat taatgatata
      241 ggattatcga atgggtacca ttcaaatcct ctaacaatgc caataattaa accatgtaat
      301 aaatatacgt atagcgtacg actaccaata taagtatata attttttctt tgttgacatt
      361 aaatttagaa acgcagtcat tgcgattaat ataattccat ataatataag tcgtttaaaa
      421 ggactgaata tactctgtcc ttcattttca agtgaagtat atggtgaact tcccaataac
      481 caatctgcat tgataggatg aatcacgtaa acgataaaaa acaaaataaa ggtaatgata
      541 gatactggta ttagtttttt atttttaaaa atagccgtat gttttttggt gaaaatgtaa
      601 cctagataaa atattgggaa aaatacgatt gtccttgaaa tgcttaagta gctatcgatg
      661 ttatctgaaa aacctgctcc aatagatata ataattgaaa ctgatagcac tttatatgga
      721 ttaaatcttc taactattac taaaatgaca tgaaagaaaa atagcgtgat caaaaaccat
      781 aacgcaaata ctgggttaaa aggatcaagt tgtaattcgt cacttttacc tgttaagaaa
      841 taataaattg aaaagaatgc aaaaaatatc atataaggta ctatcaaacg ttttgaaatt
      901 ttttctaaat agtatggttt atcaatattt ttcgcgaaat aaccagatat aaacaaaaat
      961 gttggcatat ggaaactata aataactagg tataatgctg ataaatattt atcgccacta
     1021 gtgtagggtt gtaacatatg tccaaatacg actaataata ttaaaattgc tcttgcgttg
     1081 tcaaaaaaat aatctctctc ttttaatgaa gtcatatttt atctccttta tgtccataag
     1141 actcttacta atttattata ttttgtatta gttatgcaac aaatttgtct attgtgtcga
     1201 atagtattgt aaaaattata aaaaaagcat ataatattat aaataatatg tagtggagta
     1261 gaacaacatg tataaacaac ttgaaaaact tattacactg actaacaatg acttaaactt
     1321 agtgaataga agatttggac aacgcacgga tatcacatct gaacagctag aacttctccg
     1381 tattttattt aattacgatc gcttatcaca gtatgatttg acgatgaaga ttagcaggga
     1441 acaatctata gtttcaaggt ggattaagaa attagttttg aaagggtaca tcacaagtca
     1501 acaatctagc gaagatttaa gatgtaaaga attaattttg actgatcaag cacgtacatt
     1561 aatttcacaa ataaataatg cacgttgcga attgattgaa gcaagatgtc aatgtttatc
     1621 ggaagtcgaa ttagacaatt taaatcaatt acttgataag ttaaatcaac gacgcatatc
     1681 gttgtaacac gacaatagca aacacataat ttagtgtcgt tgtgcacaca acaaaaattg
     1741 aatcatgaat tacaagcaaa agtagcggtg attgttaaaa ttgatggtaa acaatcaccg
     1801 ctatttttgc ttgtgtatgt ataaaaaagg gatcaaaggt catcccccat gattgatagt
     1861 gggggatgac ttttgatcct atgttcatgt tgcttattta tattgtggga tgtcgaagta
     1921 tttgccgact tcgccaattt tatcatagta gcctttgatg attttagcat tgatgttagc
     1981 ccaatctaca tctgtagcat attggtgtgt tcctggatgt gcaggattcc atctcatttt
     2041 gtaaagtgta ttttgaccag cttttacata tgagttgccg atgaatttag caccaccaac
     2101 gattgctttt gatactgtgt cccaaccagc ttgtttagca tatttaatac cttcacgtaa
     2161 aggatcgtta tcatatgcag caataccaaa tacgttatgg tatttcgtgt ttgagttagt
     2221 tacaactttg ttgttcacta catctgcacc tttcgctaat tgagaagtac cgttacctgt
     2281 ttctaatagg gcatgtgaga taagataaac ttcattaatg ccatacattt gagcagcttt
     2341 gttaaatgca gcaccttggt tttctaatac acctttacct tttaagaatt gattaatttt
     2401 atcaatagaa atattttgtg gttggtctaa gcgtaagaat tgatatttta atgctggatc
     2461 ttgagctaaa cgcttcgtat ccattgcatg cttaacatca ttaaagttag catctgtcca
     2521 cttacctggt acacgttgta cttgtggttt atattgtaaa ccagcttgta tttgagcaac
     2581 ttggtttaat gtcatacctg tttgattata cttaattaat tctttagcta aatcagttga
     2641 tttaatccat gctaatttac cgttagataa tttaccatag taccaagttt gtccattaat
     2701 gacttgttct ttaacaactg cgaatggttg ttcattaaat gcttttaatg agtatttagc
     2761 tgtatcagaa tttggtgtta catagtaata accattacca tttttaatta cataagtgta
     2821 gttataatct ttggcagctg atgtagttgg tttcacagca gttggtgcag ttaaatcttt
     2881 tgcatttacc caaccagtgc ggttattaat agtaccgtat aaataaacat ctttgcctac
     2941 agatacttgt ttcgttgcat taaatgtacc ttgagcaatg ttattgcctg ttaaaatgac
     3001 ttggttttta gtaccccaag gaaccattga taagccgtta tttgatttat taacagtata
     3061 tttttgagtc gttttaactt ctttgcctaa gttttgaaca tttaagtctt ttacattgaa
     3121 ccaacctaat gggatgttat ggcttgtatt gtttaataat acatacgttt cattaccatg
     3181 agcacgctct tttgttacat agaacgtacg gtctgcatat ttcgcaccgt ttttcgctgt
     3241 tttttcataa acagaagcac gaataccagt gttgtttggt ttaacttgag caatcttgct
     3301 aactgtttga gtcgtttgtg gtttagtaac agtataagct tttacagctg tttttggttg
     3361 tgctactgct tttttaggtg cagcaggtac agctaaatat gctttactta cccaaccaga
     3421 tttaccattt acagttccaa ataaatagat agatttatca atttgttgtt gcttagtcgc
     3481 tttaaaagtt tggttacctg taccagaaac tgcaccagct tcttgtttat aagtgcccca
     3541 aggtactgaa tataatttag tgcctggttt tactgtatat gtttgcatta catttacagg
     3601 tgattttgca ttgttataaa taacgtcacc ttgtttaacc caaccaatta aagttggact
     3661 attgtaatct ttaactaagt agaatttgtt tccacctaaa cttgcttctt ttgttacagc
     3721 aaatgttttt tgaacttctt tcgttggctt accagttttg tcataaactg tagtgaataa
     3781 gccattgttt ttagcattaa tttgagcaac accgtttaat gatgaaactg ttaatttatt
     3841 atttgttgta ggtgttgatg gcttaggtgt tggtgtaggc gtaggtttag cagtatcaac
     3901 taaatatgct ttacttaccc aaccagattt accattcaca gagccatata aataaattga
     3961 tttatcaatt tgttgttgct ttgaagcctt aaatgtttgg tttccagagc cagacacact
     4021 accagcaact tgtttagatg taccccaagg tactgtataa agtttcgtac caggtttgat
     4081 tgaatatgat tgatttacat ttacaggtga tttagctgtg ttgtaaacca catcgccttc
     4141 tttaacccaa ccaaatttat taccagaatt gtaatcttga acaagataga atttttgatt
     4201 acctaatgta gctgttttag atacagcaaa tgttttttga acttcattag ttgctttacc
     4261 agttttgtcg tatacagtag tatataaacc actatttgtt ggtttgattt gtgcgacacc
     4321 attgtttgca gcaactgtta atttaccagt tgatggtttc gacggtgttg ttggttttga
     4381 tggtgtagta ggggtagttg tagattgcgt accccatggc gccactttac ccatttttat
     4441 taaatatttt tcattaatta agtcatataa ttgatcataa ctataattat gacttcttaa
     4501 atatccatgt ggatcggcat ggtcagtacc acctaaatat ttacttacag cgtagtgagt
     4561 ccatactgta ccatttccat catactcagc actgtctggt tttaaaccat aatattgtaa
     4621 ttgtgtagct gcatagtcag catagttatt cattgaacgt gcaaatgaag catagtcgtg
     4681 tgtgtgtacg atttcaacat tgatgaatct agggttaccg actgcaccga caccccaaga
     4741 taagtaatcc gttggtgctg tttcgattat acgatcccca tcaacaaatg catgtacgaa
     4801 tgcgttttga tagttatttt tcatataact aatttcacca tttatcgtcg aacgatcatt
     4861 agctgtatca tgaactacga taccttcagg acgacctacg ccgttacggt atgcgtattt
     4921 agggaagtaa gatgtataat cttcttcaat tttaggtgct tttaagttat ttttacgaat
     4981 gtaatcgtta attgaagagt ttacttgtgg tttatatttt ggcaaactcg tttttggtgt
     5041 tgcagcaact gatcttggtt gtgctgaagc gctaaaagta gttactttag gtgtcgcttc
     5101 agttttagct ttaggtgctg atgtagttgc agctttaggt gctgcggttt tatattgcgt
     5161 ttcaagagct gcaggtttag cagctgattt aattaattct ggattaattt gattttctga
     5221 attatcatct tcatcatcaa ctaaactata accagcattt gtaacattag tgttagtttt
     5281 aggtgctgta gtgcttgttg actttgcaac aggctgcgta ttatttgtag tcgctgattg
     5341 attagcacga gtgtcaccat ttacttgtgc agtatcaact ttttgactta cttgagcatt
     5401 gcctgttttg ttatttgctg tttttggttg gacaatagca gggtcttgat atacttgagt
     5461 gccagaaatg ttttgcgttg gattttttac ctcagctttt gcttgttcag tagttgcttt
     5521 aactttatta ctatctaaaa cgtttttatt agtagtttga tcttgtgtcg tctcagctgc
     5581 ttgaacttga tgtgcagtga ctgctgaacc tacaagcgtt aatgcaacca ttgatggtag
     5641 tttgtaattg aattttttcg ccattctatt tattactcct aacatttatt aattattact
     5701 aacattatag tacctgtttt atatacctgt gcgtatttaa ccaatttgta atatactctt
     5761 aatttacatt ttcactgtaa aaaaatgaaa aaaattttga atctcaatgg tgacgtgaca
     5821 aatcaaatag aattgatgtc aatttaagaa ttggtttatt acaaatgttt ctaaaataga
     5881 tacaactttt tttaattaag cgaggttaac ttttttgtca tttcaatatg ctcgatgcct
     5941 tcctcaagaa atatattacc tctcatttta aagtttaaac tttcataaaa tgggatagca
     6001 tgacattggg cattcatagt agctacgtaa aaaccttcat ctttagctaa tgattctaca
     6061 gcttgcataa gcattctacc cattccttgt ccacgatgtg atttcatcac agctactcgt
     6121 tctattttga cagttgtttc attaataggg cgtattcgag cagtggcaac tggctgtcca
     6181 ttatcatatc caatgaggtg aatagattca gattcatatt catcaatttc actttcctca
     6241 gggacgcctt gttcttctac aaacactttc tttcttatat aaaagcaatc ttctaacatc
     6301 ttttgattgt ttacttttga aaacataaaa ttcctccaca tcctaacaaa atcatttaca
     6361 tttaatttac taataataac atagaataac gattgttatt ttcctcaaaa ataattagac
     6421 ataaaaaagc tataaagatg attgttgtga atcatcttta tagctaatta attattaatg
     6481 ttatagtgct tcttttctaa taattgctgt atattgccaa aagattcgca tttgagcaat
     6541 attccaatta ttcattccca ttgtaaatcc tgaaggctta aatatattca catcagttac
     6601 ttctaaagct gctgcaacta aatattgtaa tggaatgcta agtgatttca ttgtttcggt
     6661 aatgccattt tcatcatata cccatgaaaa tggtaactct gattcgaaaa catcaacact
     6721 attgaaaatt tcaggtaggg ctaaaatata acatgcacca tctacaatag gattgtcgat
     6781 actatttttg taataaatta ggagtgcttc atagttttct cgatgctcat gattgacgta
     6841 aaaaaattca cttctaaaat gagccatatc ttggctatga atgatttgtt gttctaacat
     6901 attaaacatg ccatttatat ttgcaatctt ttcgtatgtt tttctactca tgtatgatgc
     6961 tcctttcata aatagtaaat gttaatcgtc atataatatt aatttacaac accattttgg
     7021 ttatttgaag cttgtggcgc ttgttgtgtg ccaccttgat tttgatttga gttttgatct
     7081 gtagcaggtt gttgttgatt gctggaatca ctgttattag ttgaatcact gttctcgtta
     7141 gatgtcttat ctttatctgt cgtatcattt tgttcttttc tcaataaact attatctaaa
     7201 ggcgttaatg gtattaatga accataatga ttaatgacac gttgatctaa gaaatcattt
     7261 ttatcattaa taggtgataa ttctaagtct ttacgaagta agtttgcata tttttgaatg
     7321 ctttcaacac ttggatgata atagtaaata ccatttaaca tatcatcttt accttttaat
     7381 tgcgcagttt taatttcaac atcatttgtt aagtacattt ttgctaaagc tttaatttca
     7441 gagttagtta aattatgctt tgcattttta cctacaattt gaatcacgtt atcaagttta
     7501 tcaatagaat caacttcctg tgctttttgg aataaaatct taattaattc catttgacgt
     7561 tgtccacgtt ttaagtctga atcatgatgt ctagttctag caactgctaa agcctcatca
     7621 ccatttaatt tttggtaccc ttttttaatt ttaatcttac cagtatcatc tgtgttaggt
     7681 tcatttaagt cgtatggcac atcatagtat atgccaccaa gctcatttac agcctcgaca
     7741 aatgctttca tattgactct cacataataa tcaacaggta cattcatggt agcttctacc
     7801 gaatccattg cggcaattgg accaccatat gcatgtgcat gggtaatctt atcgtaatag
     7861 ccaactttag gaatgtagct gatagtatca cgtggaatac taagcattct aatttgatgt
     7921 tttgattgat taaaagtagt taaaatcata gcgtctgatc tagagtgttc agcatcctgt
     7981 cctttttttc ttcttccatc gttatcatcg atacctaaga aaagaataga gataggttgt
     8041 tcttcgggat tgactttatt atctcttaag ttggattgac gattagcatt tttgctgtct
     8101 tgagaagatt cgaatgcatc ttgggacgtt ttaaaaagta acgtagcgaa gactattgga
     8161 acaacaatga gaaccaatgc tagaaggatc aaaaagtatt ttaaaaa
//
LOCUS       DAXWFK010000111         8175 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.111, whole genome shotgun sequence.
ACCESSION   DAXWFK010000111 DAXWFK010000000
VERSION     DAXWFK010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8175
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.111"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..192
                     /locus_tag="ACGEXS_001916"
     CDS             <1..192
                     /locus_tag="ACGEXS_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Csa1 family protein"
                     /protein_id="HFF8012915.1"
                     /translation="NVKQLRKRYDIPTSKAPKLLLKGSGNLKGSSVGYKDIEFTFVEK
                     KEENIYFSDSLDYKKSGDV"
     gene            244..1014
                     /locus_tag="ACGEXS_001917"
     CDS             244..1014
                     /locus_tag="ACGEXS_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HFF8012916.1"
                     /translation="MIKRVNKLVLGISLLFLVISITAGCGMGKEAEIKKSFEKTLSKY
                     PIKNLEDLYDKEGYRDDQFDKNDKGTWIVNSQMAIQNKGEALKIKGMLLKIDRNTRSA
                     KGFYYTNEIKTEKYEVAQDNQKKYPVKMINNKFISTEEVKEENIKKEIENFKFFAQYS
                     NFKDLMNYKDGDISYNPEVPSYSAQYQLTNDDYNVKQLRKRYDIPTNKAPKLLLKGTG
                     NLKGSSVGYKKIEFTFLENKNENIYFTDSLHLEPSEDK"
     gene            1078..1848
                     /locus_tag="ACGEXS_001918"
     CDS             1078..1848
                     /locus_tag="ACGEXS_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HFF8012917.1"
                     /translation="MMKRLNKLVLGIIFLFLVISITAGCGIGKEAKIKKSFEKTLSMY
                     PIKNLEDLYDKEGYRDDEFDKNDKGTWIIGSEMATQNKGEALKVKGMVLYMNRNTKTT
                     KGYYYVNAIKNDKDGRPQENEKRYPVKMVDNKIIPTKEIKDKNIKKEIENFKFFVQYG
                     NFKDLSKYKDGDISYNPEVPSYSAKYQLTNDDYNVKQLRKRYDIPTNKAPKLLLKGTG
                     NLKGSSVGYKDIEFTFVEKKEENIYFSDGLIFKPSEDK"
     gene            1915..2682
                     /locus_tag="ACGEXS_001919"
     CDS             1915..2682
                     /locus_tag="ACGEXS_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HFF8012918.1"
                     /translation="MKRLNKLVLYISFLILVISFTAGCGIGKEAEVKKSFEKTLSMYP
                     IKNLEDLYDKEGYRDDEFDKNDKGTWIIGSEMVVQPKGERMKSKGMVLYMNRNTKTTT
                     GKYIVSETLHDEDGRPKSKDKEYPVKMVDNKIIPTKGIKDENIKKEIENFKFFAQYGS
                     FKDLSKYKDGDISYNPEVPSYSAKYQLTNDDYNVKQLRKRYKIPTNKAPKLLLKGSGD
                     LKGSSVGYKDIEFTFVEKKGENTFFTDSLHLEPSEDK"
     gene            2776..5013
                     /locus_tag="ACGEXS_001920"
     CDS             2776..5013
                     /locus_tag="ACGEXS_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8012919.1"
                     /translation="MQRDYLIRVETESMSDFKRLNSLMIGFVIKGEAHIYDENNMTQC
                     NSGDIFIINHRDLYRFQLQQDGIICYIQFQMKYLADKFDDVHCLYFHLTDATTTKNIH
                     QLRNIMARLVSTHIRHNELSKLTEQQLVIQLLMHMIHYVPRTYHSNQSILNDDKVNQV
                     CDYIELHFHEDLSLSELSEYVGWSESHLSKKFTESLGVGFHHFLNTTRIEHAKLDLTY
                     TDETITDIALQNGFSSAASFARTFKHFTHQTPKQYRGDRPAITENQQSAQHDYHDREL
                     ILLLNDYIEEMNHFIEDIEKMNYKEIAFQPTNHQLNQFNHIIQVGYLRNLLNTQYQSQ
                     LLTCHHDFQVNEVLAYDVIPYIMKKLNAPFTYDAEISNIFYDIDLCLDFLLDHNFSLT
                     MHLDQYDSRDYIDAFKIFIHHVALHVSHRKDLKFNLYVTTLHTSLIEMIDYFKALFPN
                     GGLYIHLDQATERHLPLLKRLEPHIDHFVFDANSNDAVDFNKMNDDEFKTASQMIINK
                     TNYLIDLMHRHRLKRPLILLNWNTLTGDTFITNGEYFRGGIIIEQLLKLSSKVEGIGY
                     WLNYDLHVSHCKNERDYMNSIELFHQYNGKRPVYFTALLFNKLTSNILYSDDTCIVTG
                     TDSNFQILLYDAKHFNPYLALDNQMNMRATEMIHLNINALEEGMYKIKHFTLDKENGA
                     LFNLWRKHHTIHGMDKDSIDYVNRMSFPKLEVYDIDITDTLALNIKMITNGIHLIEVK
                     RYPSS"
     gene            5164..6342
                     /locus_tag="ACGEXS_001921"
     CDS             5164..6342
                     /locus_tag="ACGEXS_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFF8012920.1"
                     /translation="MNQQLIETLKSKEGKMIEIRRYLHQHPELSFHEDETAKYIAEFY
                     KGKDVEVETNVGPRGIKVTIDSGKPGKTLAIRADFDALPITEDTGLSFASQNKGVMHA
                     CGHDAHTAYMLVLAETLAEMKDSFTGKVVVIHQPAEEVPPGGAKTMIENGVLDGVDHV
                     LGVHVMSTMKTGKVYYRPGYVQTGRAFFKLKVQGKGGHGSSPHMANDAIVAGSYFVTA
                     LQTVVSRRLSPFETGVVTIGSFDGKGQFNVIKDVVEIEGDVRGLTDATKATIEKEIKR
                     LSKGLEDMYGVTCTLEYNDDYPALYNDPEFTEYVAKTLKEANLDFGVEMCEPQPPSED
                     FAYYAKERPSAFIYTGAAVENGEIYPHHHPKFNISEKSLLISAEAVGTVVLDYLKGDN
                     "
     gene            6344..7732
                     /gene="norC"
                     /locus_tag="ACGEXS_001922"
     CDS             6344..7732
                     /gene="norC"
                     /locus_tag="ACGEXS_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorC"
                     /protein_id="HFF8012921.1"
                     /translation="MNETYRGGNKLILGIVLGVITFWLFAQSLVNVVPNLQQSFGTDM
                     GTISIAVSLTALFSGMFVVGAGGLADKIGRVKMTNIGLLLSIIGSALIIITNLPALLI
                     LGRVIQGVSAACIMPSTLAIMKTYYQGAERQRALSYWSIGSWGGSGICSLFGGAVATT
                     MGWRWIFIFSIIVAVLSMLLIKGTPETKSEITNTHKFDVAGLIVLVVMLLSLNVVITK
                     GAALGYTSLWFFGLIAIVIVAFFIFLNVEKKVDNPLIDFKLFENKPYTGATISNFLLN
                     GVAGTLIVANTFVQQGLGYTALQAGYLSITYLIMVLLMIRVGEKLLQKMGSKRPMLLG
                     TFIVVIGIALISLVFLPGIFYVISCVVGYLCFGLGLGIYATPSTDTAISNAPLDKVGV
                     ASGIYKMASSLGGAFGVAISGAVYAGAVAATSIHTGAMIALWVNVLMGIMAFIAILFA
                     IPNDDKRVKDAK"
BASE COUNT         2971 a         1132 c         1572 g         2500 t
ORIGIN      
        1 aatgtaaaac aattacgcaa aagatatgat ataccgacga gtaaagctcc aaagttattg
       61 ttaaaaggtt cagggaattt aaaaggctca tcagttggat ataaagatat tgaatttacg
      121 tttgtagaga aaaaagaaga aaatatatac tttagtgata gcttagatta taaaaaaagc
      181 ggagatgtat aatcatggct caatcagaat atgaaatcaa tcccggaaaa agagagtgat
      241 gaaatgataa aacgtgtaaa taaattagtg cttggtatta gtcttctgtt tttagtcatt
      301 agtatcactg ctggttgtgg catgggtaaa gaagcggaaa taaagaaaag ttttgaaaaa
      361 acattgagta agtatccgat taaaaatcta gaggatttat acgataaaga aggatatcgt
      421 gatgatcaat ttgataaaaa tgataaggga acatggattg taaattctca aatggcaatt
      481 caaaataaag gagaagctct aaaaataaaa ggcatgcttt tgaagataga tagaaataca
      541 agaagtgcaa aaggatttta ctatactaat gaaataaaga cggagaaata cgaagtagct
      601 caggataatc aaaaaaaata tccagttaaa atgattaata ataaattcat ttctactgag
      661 gaagttaaag aagaaaacat aaaaaaagaa atcgaaaact ttaagttttt tgcgcaatat
      721 agcaatttta aagatttaat gaattataaa gatggagata tatcatataa tccagaggtg
      781 ccgagttatt cagctcaata tcaattaact aatgatgatt ataatgtaaa acaattacgt
      841 aaaagatatg acataccaac aaataaagcg ccgaagctgt tgttgaaagg tacagggaat
      901 ttaaaaggtt catcagttgg atataaaaaa attgaattta cttttttaga gaataaaaat
      961 gaaaatattt actttactga tagtctacat cttgaaccga gcgaggataa ataatcatct
     1021 tttaaccaga atatgaaatc aatctaggac aacctaataa aaggagagag ttgtaagatg
     1081 atgaaacgat taaacaaatt agtgttaggc attatttttc tgtttttagt cattagtatc
     1141 actgctggtt gtggcatagg taaagaagcg aaaataaaga aaagttttga gaagacattg
     1201 agtatgtatc cgattaaaaa tctagaggat ttatacgata aagaaggtta tcgtgatgac
     1261 gaatttgata aaaatgataa aggtacatgg attattggtt ctgaaatggc aactcaaaat
     1321 aagggggaag ctctgaaagt taaaggtatg gtcttgtata tgaatagaaa taccaaaaca
     1381 acaaaaggat attattatgt aaatgcaata aagaatgata aagacggaag accccaagag
     1441 aatgaaaaaa ggtacccagt taaaatggtc gataataaaa tcattccaac aaaagaaatt
     1501 aaagataaaa acataaaaaa agaaatcgaa aactttaagt tctttgttca atatggaaac
     1561 tttaaagatt tgtcgaagta taaagacgga gatatttcat ataatccaga ggtgccgagt
     1621 tattcggcta aatatcaatt aactaatgat gattacaatg taaagcaatt acgtaaaaga
     1681 tatgacatac caacaaataa agcaccgaag ttgttgttga agggtacagg gaatttaaaa
     1741 ggttcatcag ttggatataa agacattgaa tttacgtttg tagagaaaaa agaagagaac
     1801 atttacttta gtgatgggtt aatttttaaa ccaagtgagg ataaataatc atgactcgat
     1861 caaaatatga aatcaatcca ggaaagataa taagaagaat aggattgtga aattatgaaa
     1921 cgattaaaca aattggtatt gtatattagt tttttgatat tagtcattag tttcactgct
     1981 ggttgtggca taggtaaaga agcggaagtt aagaaaagct ttgaaaaaac attgagtatg
     2041 tatccgatta aaaatctaga ggatttatac gataaagaag gttatcgtga tgacgaattt
     2101 gataaaaatg ataaaggtac atggattatt ggttctgaaa tggttgttca acctaaaggg
     2161 gaacgtatga aatcaaaagg tatggttcta tatatgaata gaaacactaa gactacaacc
     2221 gggaagtata tagtgagtga aacacttcat gatgaagatg gtagacctaa gagtaaagat
     2281 aaagaatatc ccgttaaaat ggtagacaat aaaatcattc caactaaagg tatcaaagac
     2341 gaaaatataa aaaaagaaat cgagaatttt aagttcttcg cgcaatatgg cagctttaaa
     2401 gatttgtcga agtacaaaga cggcgatata tcatataatc cagaggtacc gagttattca
     2461 gcaaaatatc aattaactaa tgatgactat aatgtaaaac aattacgaaa aagatataaa
     2521 ataccaacga ataaagcacc aaagttattg ttgaaaggtt cgggagactt aaaaggttca
     2581 tcagttggat ataaagacat tgaatttact tttgtggaga aaaaaggtga aaatactttt
     2641 tttactgata gtctacatct tgaaccgagt gaggataaat aatcatccac acacacgatt
     2701 caatatgaaa ttcaaatatg ttgctgtaaa ataatgtaaa ataaacgtat tcatattact
     2761 gaattgaggg atagtatgca acgtgattat ttaattcgag tagaaactga gagtatgtca
     2821 gatttcaaaa ggctcaatag tttaatgatt ggttttgtta ttaaaggtga ggcacacatt
     2881 tatgatgaaa ataatatgac gcaatgcaac agtggcgaca ttttcatcat taaccaccgc
     2941 gacttgtatc gatttcaact tcaacaagat ggcatcatat gttatatcca attccaaatg
     3001 aaatatttag cagacaagtt tgatgatgtg cattgtctat attttcactt aacagatgcg
     3061 accacaacaa agaatataca tcaactgaga aatataatgg caagactggt ttcaacacat
     3121 atccgacata atgagttgtc taaattgact gagcaacaac ttgtgattca gttgcttatg
     3181 catatgattc attatgtccc gcgtacatat cattcgaacc aaagtatctt aaatgatgat
     3241 aaagtgaatc aagtatgcga ctatatcgag ttacattttc atgaagattt aagcctttca
     3301 gaattaagcg aatacgttgg gtggtcagag agccatctgt ctaaaaagtt tacagaatcg
     3361 ctaggtgtag gattccatca tttcttaaat acgacgcgaa ttgagcatgc gaaactcgat
     3421 ttgacataca cagatgaaac cattactgat attgcattgc aaaatggctt ttcaagtgca
     3481 gcgagctttg cgagaacatt taaacacttt acgcatcaaa cacctaaaca atatcgaggt
     3541 gatcgtccag caatcactga aaaccagcaa tcggcacaac atgattatca cgaccgtgaa
     3601 ttgatattac ttttaaatga ctacattgaa gaaatgaatc attttattga agatattgaa
     3661 aagatgaact ataaagagat tgcctttcaa ccaaccaatc accaactaaa tcaatttaat
     3721 catattattc aagtgggcta tttgaggaat ttgctcaata cacagtatca atcacagttg
     3781 cttacatgtc atcatgattt tcaagtcaat gaagtattag catatgatgt gattccatat
     3841 atcatgaaaa agctcaatgc gccattcacg tatgatgcag aaatttcgaa tatattttat
     3901 gatattgatt tgtgtttaga ctttttatta gatcataact ttagtttgac catgcatttg
     3961 gatcagtatg actcacgtga ttatatcgat gcatttaaaa tttttatcca tcacgttgcc
     4021 ctgcatgtca gtcatagaaa agatttgaag ttcaacttgt atgtgacgac attgcacacg
     4081 tctttgattg aaatgattga ttattttaaa gcattattcc ctaatggtgg cttgtacatt
     4141 cacttagatc aagctacgga aagacatcta ccattattga aacgacttga gccacacatc
     4201 gaccattttg tatttgatgc caattcaaat gacgctgttg attttaataa aatgaatgat
     4261 gatgaattta aaaccgcgag tcaaatgatt attaataaaa cgaattacct tatcgactta
     4321 atgcatcgtc atcgcctaaa gcgtccactc attttactca attggaatac attgacgggt
     4381 gatacattta ttacaaacgg cgaatatttt agaggtggta tcatcattga gcaattatta
     4441 aaattaagtt ctaaagtaga gggtatcggg tattggttga attatgattt gcacgtcagt
     4501 cattgtaaaa atgaacggga ttatatgaat tctattgaac tatttcatca atataatgga
     4561 aaacgtccgg tctatttcac ggcattgcta tttaataaat taacaagcaa tattttgtat
     4621 tctgatgata catgtattgt cacggggact gattcaaatt ttcaaatatt gttatatgat
     4681 gcaaagcatt ttaatccgta cttagcgttg gacaatcaaa tgaatatgcg tgcgacggaa
     4741 atgattcatt tgaacattaa tgccctggaa gaaggtatgt ataagattaa acattttacc
     4801 ttagataaag aaaatggtgc gttatttaat ctttggcgca aacatcatac gatacatggc
     4861 atggacaagg actctataga ttacgttaat cgaatgagtt ttccgaaatt agaagtatat
     4921 gatatagata tcacggacac actggcatta aacattaaaa tgattacgaa tgggattcac
     4981 ttaattgaag taaaacgtta cccaagttca taaaatgatc acaaatcaca aattttgata
     5041 tacataattt gtgatttttt atattcaaag ctaaaattgc aaaaaattaa tggttaacat
     5101 ctctgttgtt ggcaatataa ataatgatta atcatttatg atgtaactaa ggagatgaag
     5161 gatatgaatc aacaattaat tgaaacttta aaatctaaag aaggcaaaat gattgagatc
     5221 agacgttatt tacatcagca tccagaatta tcttttcatg aagatgaaac ggcgaaatac
     5281 atcgctgaat tttacaaagg taaagatgtg gaagtagaaa cgaatgtcgg accacgtgga
     5341 attaaagtaa cgattgattc agggaaacct ggtaaaacat tagcaatccg tgcagacttt
     5401 gacgcattac ccattactga agatacagga ttatcttttg catcacaaaa taaaggtgtt
     5461 atgcacgcat gtggtcacga tgcacataca gcatacatgc ttgtattagc agagacgctt
     5521 gctgaaatga aagatagttt tacaggaaaa gtcgttgtga tacatcaacc agctgaagaa
     5581 gtaccaccag gtggtgctaa aacaatgatt gaaaatggtg tattagacgg tgttgatcat
     5641 gtattaggtg tacacgtcat gagcacaatg aaaacaggta aagtgtatta cagacctggt
     5701 tatgttcaaa caggacgcgc attcttcaaa ttgaaagttc aaggtaaagg tggtcatggt
     5761 tcatcaccac atatggccaa tgatgccatt gttgcaggta gctacttcgt cacagcgtta
     5821 caaacagttg tatctagacg actaagtcca tttgaaaccg gtgttgtcac aatcggttca
     5881 tttgacggta aaggtcaatt caatgtcatt aaagatgttg ttgaaattga aggtgatgta
     5941 cgtggattaa cagatgctac aaaagcaaca attgaaaaag aaattaaacg tttatcaaaa
     6001 ggattagagg atatgtatgg tgtaacttgc accttagaat ataacgatga ttatccagca
     6061 ttatataatg atccagagtt tactgagtac gtggctaaga cgttgaaaga agcaaacctt
     6121 gattttggtg ttgaaatgtg tgaaccacaa ccaccttcag aagactttgc atattatgct
     6181 aaagaacgcc caagtgcctt tatttataca ggtgcagcgg tggaaaatgg tgaaatttac
     6241 ccacatcatc atcctaaatt taacatttca gaaaaatcat tacttatttc tgcagaagca
     6301 gtagggacag ttgttttaga ttaccttaaa ggagataact aacatgaatg aaacgtatcg
     6361 cgggggcaac aagttaatct taggtattgt attaggtgtt attacatttt ggttgtttgc
     6421 acaatcactt gtaaatgttg taccgaattt acaacaaagt tttggtacag acatggggac
     6481 aattagtatt gcggtcagtc taactgcact attttcaggc atgtttgttg ttggagcagg
     6541 tggtctggca gataaaattg ggcgcgtgaa aatgacgaat atcggtttat tattaagtat
     6601 tattggttca gcattaatta ttattacgaa tttaccggca ttattaattt taggtcgtgt
     6661 tatacaaggt gtatcagcag cgtgtattat gccttctaca ttggccatta tgaaaactta
     6721 ttatcagggt gctgaacgtc agcgtgcctt aagttattgg tctatcggtt cttggggtgg
     6781 cagtggtatc tgttcactct tcggtggtgc agttgcgaca actatgggtt ggagatggat
     6841 tttcatcttc tcaattatcg ttgccgtact ttcaatgtta ctcatcaaag ggacgcctga
     6901 aacgaaatca gaaattacca atacacataa atttgacgtt gcagggctaa ttgttctagt
     6961 agttatgttg ctaagtttaa acgttgtcat tactaaaggt gcagcacttg gttacacatc
     7021 attatggttc tttggtttga ttgcaatcgt aattgtagca ttctttattt tcttaaatgt
     7081 tgagaaaaaa gtagataatc cacttattga ttttaaatta tttgaaaata aaccatatac
     7141 aggtgcaacg atttcgaact tcttattaaa cggtgttgca ggtacattaa ttgtagcgaa
     7201 tacattcgtg caacaaggtt taggttatac agcattgcag gcaggatact tatcaattac
     7261 ttatttaatc atggtgttat tgatgattcg agttggtgaa aaattattac aaaaaatggg
     7321 ttctaagcga ccaatgttat taggtacatt cattgtggtc attggtattg cacttatttc
     7381 attagtattc ttaccaggca tattttatgt tatcagttgt gtcgtaggat atttatgttt
     7441 cggactaggc ttaggtattt atgcaacacc ttctacagat acagctattt cgaatgcacc
     7501 gttagataaa gttggcgttg cttcaggtat ttataaaatg gcttcatcac ttggtggcgc
     7561 attcggtgtc gcaattagtg gtgctgtata tgctggtgca gttgctgcaa cgagcattca
     7621 tacaggtgcg atgattgcac tttgggttaa cgtattaatg ggaatcatgg catttatcgc
     7681 aattttattc gcgattccta atgatgataa acgtgtcaaa gatgcgaaat aataacgatg
     7741 acacgccaag tgttttgata atgatataaa tgaaagtaaa gcaaattaaa tgaggaaatg
     7801 tatagagcag tgcatataga taaatctatg attggattaa cactaatata aatataaatg
     7861 aaaaaccatc cacttaaatt tttgataagt taggtggatg gttatttatt gcgttgttct
     7921 aaaaatttgg acatttatat ggaaagaact atatataatg tgaaggttat ggcaattcgc
     7981 ttcaatgaag gcgacgtgta aaagtgtata ttaaacgttt cggggtcaga ctccgagaac
     8041 agtgtccgtc atgcttaccg catactaatg aagcattatt atatcagtgt ttatagccga
     8101 tttaagataa taaattttgc taaacaaaaa agccaaccca tgaatgttgg attggctttt
     8161 tacatgcatc tgaat
//
LOCUS       DAXWFK010000112         8150 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.112, whole genome shotgun sequence.
ACCESSION   DAXWFK010000112 DAXWFK010000000
VERSION     DAXWFK010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8150
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.112"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..291
                     /locus_tag="ACGEXS_001923"
     CDS             <1..291
                     /locus_tag="ACGEXS_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HFF8012922.1"
                     /translation="TLISIMLYFILKDENIVEIQLLVTMSYQYILGVLLGEVLQSSWT
                     IAKLTFQRPSPQWDGSALKELSHVPIFIYSTIWIIFNLYAVNILLKYTHLIN"
     gene            complement(371..1015)
                     /locus_tag="ACGEXS_001924"
     CDS             complement(371..1015)
                     /locus_tag="ACGEXS_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFF8012923.1"
                     /translation="MLTEEKEDYLKAILTNNGDKNFVTNKILSQFLNIKPPSVSEMVG
                     RLEKAGYVETKPYKGVRLTEDGLTHTLDIIKRHRLLELFLIEILKYNWEEVHQEAEIL
                     EHRISDLFVERLDSLLNFPETCPHGGVIPRNNEYKEKYITTILNYEPGDIVTIKRVRD
                     KTDLLIYLSSKDISIGNEVEIVSKDEMNKVIIIKRNDNVIIVSYENAMNMFAEK"
     gene            1137..1880
                     /locus_tag="ACGEXS_001925"
     CDS             1137..1880
                     /locus_tag="ACGEXS_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFF8012924.1"
                     /translation="MLETKDLNLFLGNKHVLKNISLSIPVRGEIIGIMGPNGAGKSSL
                     IKSLIGEFNATGTKLLYNKPIQQQLQHITYIPQKAHIDLDFPISVEQVILSGCYKEIG
                     WFRRPNKSARDKLKQLLSDLELESLRHRQISELSGGQLQRVLVARALMSESEVYFLDE
                     PFVGIDFSSEKLIMTKIENLKQQGKLILIIHHDLSKAKQYFDRIILLNQTLRYFGDSE
                     EAMSVTRLNETFMSSTDCSDPSQRSNITC"
     gene            1874..2710
                     /locus_tag="ACGEXS_001926"
     CDS             1874..2710
                     /locus_tag="ACGEXS_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFF8012925.1"
                     /translation="MLEFVEHLFTYQFLNRALITSIIVGIVCGTVGSLIVLRGLSLMG
                     DAMSHAVLPGVALSFLFGIPMFVGALITGMIASIFIGYITSSSKTKPDAAIGISFTAF
                     LASGIIIISLINTTTDLYHILFGNLLAITNSAFLTTIVIGSIVLILIIIFYRPLMIST
                     FDPTFSRMSGLNTTLLHYFVMLLLSLVTVASIQTVGIILVVALLITPASTAFLISKKL
                     YSMMIIASLISVISSIVGLYYSYIYNIPSGATIVLCTFVIYIITLFFTKFTNRKKRGS
                     LT"
     gene            2707..3636
                     /locus_tag="ACGEXS_001927"
     CDS             2707..3636
                     /locus_tag="ACGEXS_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFF8012926.1"
                     /translation="MKKLVPLLLALLLLVAACGTGGKQSSDKSNGKLKVVTTNSILYD
                     MAKNVGGDNVDIHSIVPVGQDPHEYEVKPKDIKKLTDADVILYNGLNLETGNGWFEKA
                     LEQAGKSLKDKKVIAVSKDVKPIYLNGEEGNKDKQDPHAWLSLDNGIKYVKTIQQTFI
                     DNDKKHKADYEKQGNKYIAQLEKLNNDSKDKFNDIPKEQRAMITSEGAFKYFSKQYGI
                     TPGYIWEINTEKQGTPEQMRQAIEFVKKHKLKHLLVETSVDKKAMESLSEETKKDIFG
                     EVYTDSIGKEGTKGDSYYKMMKSNIETVHGSMK"
     gene            complement(4796..6838)
                     /locus_tag="ACGEXS_001928"
     CDS             complement(4796..6838)
                     /locus_tag="ACGEXS_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFF8012927.1"
                     /translation="MEIFETILIFIAVVILSSFVHTFIPKVPLAFIQIFLGMLLFITP
                     IPVQFNFDSELFMVTMIAPLLFVEGVNVSRVHLRKYIKPVMMMALGLVITTVIGVGLF
                     IHWIWPDLPIGAAFAIAAILCPTDAVAVQAITKGKVLPKGAMTILEGESLLNDAAGII
                     SFKIAVGVLVTGAFSLVDAVQLFLIASIGGAVVGLLIGMALVRFRLTLMRRGYENINM
                     FTIIQLLTPFVTYLIAELFHASGIIAAVVAGLVHGFERDRIMQVRTQLQMSYNHTWNI
                     LGYVLNGFVFSILGFLVPEVIIKIIKTEPHNLIFLIGITIVVALAVYLFRFVWVYVLY
                     PYFYLAISPFQKMMTKNDDDNPTTEKPPKRSLYALIMTLCGVHGTISLAIALTLPYFL
                     AGHHAFTYRNDLLFIASGMVIISLVVAQVLLPLLTKPAPKTVIGNMSFKVARIYILEQ
                     VIDYLNQKSTFETSFKYGNVIKEYHDKLAFLKTVEKDDENSKELERLQKIAFNVETKT
                     LESLVDEGQITNSVLENYMRYAERTQVYRQASLIRRMIVLLRDALLKRRVQTRVNSAS
                     SLSVTDNLMELNKINKLVHYNVVSRLSKETTKDNTLEVGMVCDGYLMRIENLTPSNFF
                     NSASEDTITKIKLNALREQRRILRDLIDTDEVSEGTALKLREAINYDEMVIVDSMT"
     gene            complement(7178..7615)
                     /gene="mnhG2"
                     /locus_tag="ACGEXS_001929"
     CDS             complement(7178..7615)
                     /gene="mnhG2"
                     /locus_tag="ACGEXS_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit G"
                     /protein_id="HFF8012928.1"
                     /translation="MEITKEIFSLIAAVMLLLGSFIALISAIGIVKFQDVFLRSHAAT
                     KSSTLSVLLTLIGVLIYFIVNTGFFSVRLLLSLVFINLTSPVGMHLVARAAYRNGAYM
                     YRKNDAHTHASILLSSNEQNSTEALQLRAEKREEHRKKWYQND"
     gene            complement(7590..7892)
                     /gene="mnhF2"
                     /locus_tag="ACGEXS_001930"
     CDS             complement(7590..7892)
                     /gene="mnhF2"
                     /locus_tag="ACGEXS_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit F"
                     /protein_id="HFF8012929.1"
                     /translation="MIQTITHIMIISSLIIFGIALIICLFRLIKGPTTADRVVTFDTT
                     SAVVMSIVGVLSVLMGTVSFLDSIMLIAIISFVSSVSISRFIGGGHVFNGNNKRNL"
     gene            complement(7889..>8150)
                     /gene="mnhE2"
                     /locus_tag="ACGEXS_001931"
     CDS             complement(7889..>8150)
                     /gene="mnhE2"
                     /locus_tag="ACGEXS_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit E"
                     /protein_id="HFF8012930.1"
                     /translation="STINYILFKTKDMNPGLLSYETRLTSDWSITFLTILIIITPGST
                     VIRISQDSKKFFIHSIDVSEKEKDSLLRSIKHYEDLILEVSR"
BASE COUNT         2836 a         1379 c         1202 g         2733 t
ORIGIN      
        1 actttaattt ctatcatgtt gtattttata ttaaaagatg aaaatatagt agaaattcaa
       61 ctacttgtca caatgagtta tcaatatatt ttaggtgtat tattaggcga agttctacaa
      121 tctagttgga cgattgctaa attgactttc cagcgtccga gtccacagtg ggatggtagt
      181 gcacttaaag aactcagtca tgtacctatc ttcatttata gtacaatctg gattatcttc
      241 aatctttatg cagtgaatat tttactcaaa tatactcact tgataaatta actaataaaa
      301 aagaagccta aagcaattat ccatatgtct ttatcagttc aatcatggat atctttatgg
      361 cttctttatt ttatttttca gcaaacatgt tcattgcatt ttcgtaactg acaataatta
      421 cattatcatt acgtttaatg ataattactt tattcatttc atctttcgat acaatttcca
      481 cttcattacc aatagaaata tctttactag acaaatatat tagcaaatcg gtcttatctc
      541 tcacacgttt gattgtaacg atatcaccag gttcataatt caaaatcgtt gttatatatt
      601 tctctttata ttcattattt ctaggaatca caccgccgtg cgggcaagtt tctgggaaat
      661 ttaacaggct atccagcctt tcaacaaata aatctgaaat tcgatgttct aaaatttctg
      721 cttcttgatg tacttcttcc caattatatt tcaatatttc tattaaaaat aattctaata
      781 gtcgatgtct cttaatgata tcaagcgtat gcgttaaacc atcctctgtt aatctaacac
      841 ctttgtatgg ttttgtttca acatagcctg ctttttcaag acgtcctacc atttcactta
      901 cagatggagg cttaatattt aaaaattgag ataagatttt atttgtcaca aagtttttat
      961 cgccattatt cgtaaggatt gcctttaaat agtcctcttt ttcttcagtt aacatacttt
     1021 cacctcacat acattgtcta tattatatca cgaatttact tgacatgata aatattctca
     1081 gtttattata caattaatta ggttagccta aacttttaat taggaggtat aaacgtttgt
     1141 tagaaacaaa agatttaaat ctgtttttag gtaataagca tgtacttaaa aacatttcct
     1201 tatcgatacc agtacgcggc gaaataattg gtatcatggg cccgaatggt gctggtaaat
     1261 cttcccttat caagtcttta attggtgaat ttaatgctac cggtactaaa ttgttatata
     1321 acaaacctat acaacaacaa ctgcaacata ttacatatat tccacaaaaa gcacatattg
     1381 atttagattt tcctataagt gtggaacaag tgattttatc aggttgctac aaagaaattg
     1441 gatggtttag acgacctaat aaatcagcaa gggataaact caaacagtta ttaagcgatt
     1501 tagaattaga atctttacgt catcgacaaa tttcagaatt aagtggtgga caattacaac
     1561 gtgtgctagt agcaagagca ttgatgtccg aaagtgaagt ttattttcta gatgagccgt
     1621 ttgtcggaat tgattttagt agcgaaaaat taatcatgac aaaaatcgag aacttaaaac
     1681 aacaaggaaa acttattctt atcatccacc atgatctatc aaaagcaaag caatactttg
     1741 atcgcattat tctattaaat caaacattac gatactttgg tgatagtgaa gaggctatga
     1801 gtgtcactcg cttaaacgaa acatttatga gtagcactga ctgtagtgac cctagtcaaa
     1861 ggagcaatat aacatgttag agtttgtcga acatttattt acatatcaat tcttgaatcg
     1921 agcattgata acttcaatta ttgtagggat agtttgtggt acagttggta gtttaattgt
     1981 attacgtggt ctttcattaa tgggagatgc aatgagtcac gcagtattac ctggtgttgc
     2041 cctatcattc ttatttggta ttccaatgtt tgtaggcgca cttataactg gtatgatcgc
     2101 aagtattttt atcggttata tcacatctag tagtaaaacg aaacctgacg ccgctattgg
     2161 aattagtttt accgctttct tagcttcggg gattattatt attagcttaa taaacactac
     2221 gacagatttg taccatattc tgtttggtaa tttactggca atcacaaata gtgcattttt
     2281 aacaactatt gtgattggtt caatcgtcct tattctaatc attattttct atcgtccatt
     2341 aatgatttct acattcgatc caacgtttag tagaatgagt ggtctaaata cgacgttatt
     2401 acattacttt gtgatgttgt tactctcatt agtaacagta gcaagtattc aaacggtagg
     2461 tattattctt gtagttgcat tattaattac acctgcatct acagcatttt taattagtaa
     2521 aaaactatac tcaatgatga ttatagcaag tttaataagt gtcataagtt caattgtcgg
     2581 actttattac agctatattt acaacattcc tagtggtgcg acaatcgtac tttgtacgtt
     2641 tgtaatttat atcatcacat tatttttcac aaaatttacg aatagaaaga aacgaggaag
     2701 tttaacatga aaaaattagt acctttatta ttagccttat tacttctagt tgctgcatgt
     2761 ggtactggtg gtaaacaaag cagtgataag tcaaatggca aattaaaagt agtaacgacg
     2821 aattcaattt tatatgatat ggctaaaaat gttggtggag acaacgtcga tattcatagt
     2881 attgtacctg ttggtcaaga tcctcatgaa tatgaagtta aacctaaaga tattaaaaag
     2941 ttaactgacg ctgacgttat tttatacaac ggattaaatt tagagactgg taacggttgg
     3001 tttgaaaaag ccttagaaca ggctggtaaa tcattaaaag ataaaaaagt tatcgcagta
     3061 tcaaaagatg ttaaacctat ctatttaaac ggtgaagaag gcaacaaaga taaacaagat
     3121 ccacacgcat ggttaagttt agataacggt attaaatacg taaaaacaat tcaacaaaca
     3181 tttatcgata acgacaaaaa acataaagca gattatgaaa agcaaggtaa caaatacatt
     3241 gctcaattgg aaaaattaaa taatgacagt aaagacaaat ttaatgacat tccaaaagaa
     3301 caacgtgcca tgattacaag tgaaggtgcc ttcaagtact tctcaaaaca atacggtatt
     3361 acaccaggtt atatttggga aattaacact gaaaaacaag gtacacctga acaaatgaga
     3421 caagctattg agtttgttaa aaagcacaaa ttaaaacact tattagtaga aacaagtgtt
     3481 gataagaaag caatggaaag tttatctgaa gaaacgaaga aagatatctt tggtgaagtg
     3541 tacacagatt caatcggtaa agaaggcact aaaggtgact cttactacaa aatgatgaaa
     3601 tcaaatattg aaactgtaca cggaagcatg aaataacacg ctgtgtttta atgaagtaag
     3661 atgaattgat gttgatgcaa cctaaaatat tggtatctcc aatattttag gccacacatc
     3721 aacataacaa agtcgaaggc taatagtccc atatcgtgcg ttaaatatat attaccctcc
     3781 tattaatata tataccgttc ccgatcgcac gatatggtgg tattagaact tctctttgaa
     3841 cgaaagagaa aagctagaag ttcttatgca gttttaatta aactgtaaac atttgtcact
     3901 ctttaaatca aagagtaaag ttaaaagctt tatgtggttt tgattaaact gcgaacagct
     3961 gcttctcttt gaacgaaaga gaaaagctag aagttcttat gcagttttaa ttaaactgta
     4021 aacatttatc actctttaaa tcaaagagta aagttaaaag ctttatgtgg ttttgattaa
     4081 actgcgaaca gctgcttctc tttgaacgag agagaaaagc tagaagttct tatgcagttt
     4141 taattaaact gtcgttccct tcatctcttt taaccacaga gatgcgttag aagttcttct
     4201 aatacaattt atacaacgcc attccctaca cactcttata aaagagattc acgcgcgtca
     4261 ataaattgta ttacatacta actaaaaagc ttttcttaat cgtactaacg aagttagagg
     4321 ttcttatgtg attggacaac tgttctccct actagacttt tagcatagct ggttaaaagt
     4381 actattacag attgttcaat tgtatgcccc tttcctttga acggaatatt aaaagctatg
     4441 tgcagtaact tttaataggc ataccacttt tgcatcaagc tataaataat cgcatacttt
     4501 gatatttaat ctggcctatc aaagtaaaca tctatggttt cctcccccca tagatcctta
     4561 agccacactc atccgatgtc atatgagtgt ggctattttt tgtgaataca cttaacttca
     4621 cctttaaatg acttcgctta atctatctcc tccaattttt aaaacttatt tcaattaaat
     4681 ctcaaatatt aataattcgt atagtttaaa actatctatt caattcccct aaaataaaag
     4741 ggattgatga aaagcccttc agtttttcat caatcccttt tagcataatt aggaactacg
     4801 tcatactatc tacaataacc atttcatcat aattgatggc ttctcttagt tttaacgctg
     4861 taccttctga tacttcatct gtatcaatca aatcacgtaa aatgcgacgt tgttctctca
     4921 atgcatttaa tttaatttta gtaatcgtat cttcacttgc tgagttgaag aaatttgatg
     4981 gtgttaagtt ttcaattcgc attaaataac cgtcacaaac cattccaact tcaagtgtat
     5041 tatcttttgt tgtttcctta gacaaacgac taaccacatt ataatggact aatttattaa
     5101 ttttatttaa ttccattaag ttatccgtaa cactaagtga agatgcggag ttcactctcg
     5161 tttgtactct tcgttttaat aaagcatctc gtaataatac aatcattctt cttattaatg
     5221 atgcttgtct atatacctgt gttctttcag cataacgcat atagttttca agtacactat
     5281 tcgttatttg tccttcatct actaaagact ctaatgtttt tgtttctaca ttaaaagcaa
     5341 ttttttgtag acgttctaat tctttagagt tttcatcatc tttctctaca gtttttaaaa
     5401 atgctaattt atcatgatat tctttaatca cgttaccata tttaaaactt gtttcgaaag
     5461 tagatttttg atttagataa tcaataactt gttctaatat ataaattcta gcaactttaa
     5521 acgacatatt gccaattact gttttaggtg caggtttcgt taataatggc aataatactt
     5581 gcgcaactac caaactaata ataaccatac cagatgcaat aaataataag tcatttctat
     5641 acgtaaaagc atgatgccct gctaaaaaat acggtaacgt taatgcgatt gctaaagaaa
     5701 ttgttccatg cacaccacat aacgtcataa ttaaagcgta taaacttcgc tttggtggtt
     5761 tctcagtcgt tggattatca tcatcatttt tagtcatcat tttttggaat ggactgatgg
     5821 ctaaataaaa ataaggatat aagacataaa cccaaacaaa tctaaataga tagacagcta
     5881 aagcaacaac aatagtgatg cctattaaaa agattaaatt gtgcggttct gttttgataa
     5941 ttttaataat aacttcaggt actaaaaatc ctaatattga aaaaacaaag ccatttaaaa
     6001 cataacctag tatattccat gtatgattgt aactcatttg cagttgtgta cgtacttgca
     6061 taattctgtc acgttcgaaa ccatgtacaa gtcctgcaac tactgctgca atgattcctg
     6121 atgcgtgaaa caattcagca attaaatacg taacaaatgg tgttaacaat tgaataattg
     6181 taaacatatt aatgttttca tatcctcgac gcatcaatgt taatcggaac cttactaatg
     6241 ccatacctat aagtaaacca accactgcgc caccaattga tgcaattaaa aacaactgaa
     6301 cagcatcaac aagtgaaaaa gcacctgtaa ctaatactcc aacagctatt ttaaatgaaa
     6361 taataccagc agcatcattc aataatgact caccttcaag aattgtcatt gctccttttg
     6421 gcaagacctt tcctttagtg attgcttgca ctgctactgc atcagtagga caaagaatgg
     6481 cagcaattgc aaatgctgct ccaataggta aatctggcca aatccaatga ataaataaac
     6541 ctacacctat cacagtagta atgactaatc ctaatgccat catcatcact ggcttaatat
     6601 atttccttaa atggactcta gaaacattaa caccttctac aaataacaaa ggcgcaatca
     6661 ttgttaccat aaacaattca gaatcaaaat taaattgaac agggattggg gtaataaata
     6721 gtaacatgcc caagaaaatt tgtataaatg ctaggggtac tttaggtatg aaagtatgga
     6781 caaacgaact tagtatcaca acagctataa atataagaat tgtttcaaat atttccaaac
     6841 tttcacctct ctaaaaagta ttgtttaatt gaaaattaag tatcacatct cgttgtaatt
     6901 atactttaga ggataaattg agttagcgac cacaaaagca ctttaatata gatatatgtc
     6961 tacgattgca gtacttaaat ttgcaattat ttaattttat tttatcacta attgtttgta
     7021 taaataaaca acttgctttc acataacaac attaacttat aatacaaaaa atgagcacct
     7081 taaaatcgac taaccaattt caaagtactc ttttaatgat taattttgaa aacagatttt
     7141 caaaagcatt gttatgctta acaatttagc caacacttca atcgttttga taccatttct
     7201 tacgatgctc ttctcgtttt tcagcacgta attgtaatgc ttctgtagag ttttgttcat
     7261 ttgaacttaa taatattgat gcatgtgtgt gagcatcatt ttttcgatac atataagcgc
     7321 cgttgcgata agcagcgcga gcgactaagt gcatgccgac tggtgaagtt aaattaataa
     7381 aaacaagtga cagtaataaa cgcacactga aaaatcctgt attcacaata aaataaatta
     7441 atacaccgat taaagttaat aacacggata aagttgaact ttttgtcgca gcgtgacttc
     7501 ttaagaaaac atcttggaat ttcacgatac ctattgcact aataagagca ataaaactac
     7561 ctaacaacaa catcacagca gcaataagac taaagatttc ttttgttatt tccattaaac
     7621 acatgccccc caccaataaa gcgtgatatt gaaacagaac ttacaaaaga tataatggca
     7681 atgagcatga ttgaatctaa gaaagaaacg gtgcccataa gtacacttaa cacacccaca
     7741 attgacatta cgacagcact tgttgtatca aatgtaacga cacgatctgc tgttgtaggt
     7801 cccttgatta atctaaataa acagatgatt aatgcaattc caaaaataat gagtgaacta
     7861 ataatcataa tatgtgttat tgtttgtatc atcgcgacac ctccaatatt aagtcttcat
     7921 aatgcttaat acttcttaac aaactatctt tttctttttc tgacacgtcg atactatgaa
     7981 taaaaaactt tttagagtct tgagaaattc gtattactgt agaccctgga gttataataa
     8041 ttaaaattgt taaaaatgtt attgaccaat cacttgttag tcttgtttca tatgaaagta
     8101 atccagggtt catatctttt gttttaaaaa gaatataatt aatcgtgcta
//
LOCUS       DAXWFK010000113         8011 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.113, whole genome shotgun sequence.
ACCESSION   DAXWFK010000113 DAXWFK010000000
VERSION     DAXWFK010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8011
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.113"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            331..1467
                     /locus_tag="ACGEXS_001932"
     CDS             331..1467
                     /locus_tag="ACGEXS_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003712 - transcription coregulator
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAP domain-containing protein"
                     /protein_id="HFF8012931.1"
                     /translation="MSVNARDLLVLHTNVNRLVGEEIFANKCLANNDVQIMNSIKKLI
                     EAELLTTTNDFEVSIYKKTRPELQSILKSFGIKTTGNKPDLIKRIDDNFHIINNLDLP
                     YVYIPTKKGEEILKKTEYLTSFIQSYGEISLERAYYLVENYIDENCDDKVAEIYKFEF
                     QRKYDNGEFDFNHGYNFELNMLIDHYKRDVKDYDNARKYSNIYLYFGLRDFLKKLMSN
                     YSYYDSKGNIDLNEIQNDLNRFINSSASGMYERLIYNENLSNNIMFELFKKDTQDYSD
                     LEEQLIEKFINYVVSNVKKESRSNTLIELSKILENGYTIDKEEFKKEDDYLSKYIFTD
                     IDYLKKLESKINVAIDIRSGEIHLVLDDDSLDILIQNQKYGNEF"
     gene            1510..1993
                     /locus_tag="ACGEXS_001933"
                     /pseudo
     CDS             1510..1993
                     /locus_tag="ACGEXS_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837446.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            2154..2258
                     /locus_tag="ACGEXS_001934"
     CDS             2154..2258
                     /locus_tag="ACGEXS_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012932.1"
                     /translation="MLLLERTSMSDFEMLMVVLTIIGLVLISTQDHKK"
     gene            complement(2298..2372)
                     /locus_tag="ACGEXS_001935"
                     /pseudo
     CDS             complement(2298..2372)
                     /locus_tag="ACGEXS_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500661.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2758..3849)
                     /locus_tag="ACGEXS_001936"
     CDS             complement(2758..3849)
                     /locus_tag="ACGEXS_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFF8012933.1"
                     /translation="MFTYFKSAFKNAKPQLLITLIYALIAFAVIAVVYLLANFQLAKY
                     AQTIAIYSQFGQKPPVDAYLKVIAVLLIAAVVSLFVLVQIFIGITNVMKRAMSHEKVK
                     FTDLFIAFKKGNYLKSVLIGLVSIAMIIVLSLLTSLLYKLFSPVSEMIMNSVQSSYAD
                     STHLIGIAITTQSIIIIVVLLIKAIITWLLLIPIFNFMTSFVESTNDKVKTHLANGFK
                     AMKNGQKTFFKFFIGILLLNLIIILFKTPVGYLISFNTQSLSQSVAENIIRVYTVMTI
                     ILFVVIHAIILMGIVQYYLKRGQKITKDKVKTADKNKKVVTEPKNTKVENDKVTTSVE
                     TKTEKAQDSLNDNTTKTMTSDKPEDNQPK"
     gene            complement(4115..5095)
                     /gene="czrB"
                     /locus_tag="ACGEXS_001937"
     CDS             complement(4115..5095)
                     /gene="czrB"
                     /locus_tag="ACGEXS_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc efflux transporter CzrB"
                     /protein_id="HFF8012934.1"
                     /translation="MSHSHHHHDHMHSHVTTNNKKVLFISFLIIGLYMFIEIIGGLLA
                     NSLALLSDGIHMFSDTFSLGVALVAFIYAEKNATTTKTFGYKRFEVLAALFNGVTLFV
                     ISILIVFEAIKRFFVPSEVQSKEMLIISIIGLIVNIVVAFFMFKGGDTSHNLNMRGAF
                     LHVIGDLLGSVGAITAAILIWAFGWTIADPIASILVSVIILKSAWGITKSSINILMEG
                     TPSDVDIDEVITTIKKDSRIQSVHDCHVWTISNDMNALSCHVVVDHTLTMKECELLLE
                     NIEHDLLHLNIHHMTIQLETPNHKHDESIICSGTHSHSHNHHAHHHAHVH"
     gene            complement(5097..5417)
                     /gene="czrA"
                     /locus_tag="ACGEXS_001938"
     CDS             complement(5097..5417)
                     /gene="czrA"
                     /locus_tag="ACGEXS_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)-responsive metalloregulatory
                     transcriptional repressor CzrA"
                     /protein_id="HFF8012935.1"
                     /translation="MSEQYSEINTDTLERVTEIFKALGDYNRIRIMELLSVSEASVGH
                     ISHQLNLSQSNVSHQLKLLKSVHLVKAKRQGQSMIYSLDDIHVATMLKQAIHHANHPK
                     ESGL"
     gene            5569..6234
                     /locus_tag="ACGEXS_001939"
     CDS             5569..6234
                     /locus_tag="ACGEXS_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFF8012936.1"
                     /translation="MDILVIGANGGVGSLLVQQLAKENVPFTAGVRQSDQLNALKSQG
                     MKAILVDVENDSIETLTETFKPFDKVIFSVGSGGNTGADKTIIVDLDGAVKSMIASKE
                     ANIKHYVMVSTYDSRRQAFDDSGDLKPYTIAKHYADDYLRRSGLNYTILHPGALTNAA
                     GSGKIEAAQYFDGKGEIPREDVATVLKEIVTSNHFNHQEFQIISGDQDIKDALTQFEN
                     ETD"
     gene            6669..7607
                     /locus_tag="ACGEXS_001940"
     CDS             6669..7607
                     /locus_tag="ACGEXS_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HFF8012937.1"
                     /translation="MPLFLQPILKTKLWGGQRLSEFGYQLDNDTTGECWCVSAHPNGT
                     SEIINGPYQGQTLDRIWSEHRELFGDFPSKDFPLLTKIVDARESLSIHVHPDNSYAYE
                     HENGQYGKSECWYIIDAEEDAEIVIGTLAESREEVANHVQHGTIESILRYIKVKPGEF
                     YFIPAGTVHTISSGILAYETMQSSDITYRLYDFNRQDNQYNDRPLNIEKALDVIQYNA
                     PLPNILPESEIIENHKCTHIVSNDFFTLVKWEISGTLNYMKPREFCLVTVLEGEGQMI
                     VDGEIFKLTTGTNFILTSEDLDSVFEGDFTLMISYV"
     gene            7638..7868
                     /locus_tag="ACGEXS_001941"
     CDS             7638..7868
                     /locus_tag="ACGEXS_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012938.1"
                     /translation="MKQFLYIALVCGVIAGLGAFLHIPQYPSMTIPRIVAILGIISAM
                     LTFKDKQISASLKFSALLINVLPLCGTFVASN"
BASE COUNT         2883 a         1051 c         1406 g         2671 t
ORIGIN      
        1 attatgaaaa tggcttttag atgttgtttt aaaaataaac actttgaaat ataacctctt
       61 gctgatagat taagaaaata tattcttgaa ttttaagggg ggataattac aatcttagtt
      121 cttttaactg gtactattat caatgaatca taaaattcga aacatacgag tttttagact
      181 atgagagaca cattcatttg cattgagaat cattttttta gtgtataatc tatgtaaatc
      241 gaattagaat atagagattt tgtttgttcg gatgtatttt ttattcatac ttaataaatt
      301 tgaaaagcaa aaatgaggag atgaaagcta gtgagcgtaa atgcacgtga tttattagta
      361 ttacacacta atgtaaatag attggttgga gaagagatat ttgctaataa gtgccttgct
      421 aataatgatg ttcaaattat gaattcaata aaaaaattaa ttgaagctga gttattaaca
      481 acgactaatg attttgaagt tagtatatat aaaaagacaa gacctgaatt acaaagtatt
      541 ttaaaaagtt ttggtataaa aacaacaggt aataaacctg acttaattaa acgtattgac
      601 gacaattttc atattattaa taacttagat ttaccatatg tatatatacc aactaagaaa
      661 ggagaagaaa ttttaaagaa aacagagtac ttaacctctt ttatacaaag ttatggtgaa
      721 atttctcttg agcgtgctta ttatttggtt gaaaactata tagatgaaaa ttgcgatgat
      781 aaagttgcag aaatatacaa gtttgaattt caaagaaaat atgacaatgg cgagtttgat
      841 tttaatcatg gatataattt cgaattgaat atgttgatag atcactataa aagagatgta
      901 aaagactacg ataatgccag aaagtattca aatatttatc tttactttgg tttgagagat
      961 ttcttaaaaa aattaatgag taattattca tattacgata gtaaagggaa tatagatttg
     1021 aacgaaatac aaaacgatct gaatagattt atcaactcta gcgcttctgg tatgtacgag
     1081 cgattaatat ataatgaaaa tttatccaat aatattatgt ttgaattatt taaaaaggac
     1141 acacaagatt atagtgattt ggaagaacaa ttgattgaaa agttcataaa ctatgtagtg
     1201 tctaatgtaa aaaaagaaag taggagtaat actcttatag agttatcaaa aattttagag
     1261 aacggatata caattgataa agaagaattt aaaaaagaag atgattatct ttctaagtac
     1321 atatttactg acatagatta tttgaaaaag ttagaatcaa aaataaacgt tgctattgat
     1381 attcgaagtg gagaaattca tttggtatta gatgatgata gccttgatat attaatacaa
     1441 aatcaaaaat acggcaatga gttttgagtc ataactaaat ataatatgtt gaagagaggt
     1501 tttatttaaa tggctaaaat tggttatgat cgtgtatcaa cgaaagatta atatcgatac
     1561 acttaaagaa tttgggtgtg ggtgtgtata tagtgaaaga ataagtggtg tcaaacgtac
     1621 agagcttgtt aagtgtttag attatttacg agagagcgac acattagttg tctatcaact
     1681 tgatcggtta ggtagaacga caaaactatt aattgaatta tcacaatgat tcgatgataa
     1741 cggaattgac ttacaaatta gtaacatgaa catttcaacg aaagacacaa tgggcaaaat
     1801 gttttttacg atgatgagtg catttttcag gattagaagt taatttacta tgtgagtgta
     1861 taaaatagac ttagcagcaa caagagcgag aggccgaaaa agcgggcgcc cctctttacc
     1921 agagaataaa aaatgagaaa ttaaattttt atatgatgaa caaacgataa caggggaaga
     1981 aatagctagt tagacaaggg tatgtcactc aactgtttat cgagttgatt aagaaatgaa
     2041 aaaacttata ctatgaatta ctgtttaaaa gtgtgcatgt tataatattt attgagcaag
     2101 ttggatagat ggtggctaat ctcttaataa aggggtgatg cctatggtta tagttgttac
     2161 tcctagaaag gactagcatg tctgattttg aaatgcttat ggttgtatta acaatcattg
     2221 gtttagtatt gattagtact caagaccata aaaaataacc ttctattcgc tttgaccggc
     2281 atttttgaag gctattttta aataaaatat aaggtcaccg tctttttaac gggctcatta
     2341 gggtaacatg tttcctagtg ttgccctttt tgtgtttcaa gagttaatga ttttcatctt
     2401 ttgcttctac ttactacaaa tatattttaa cacatttttc ttatgaattg tagttctgaa
     2461 cataatcaga attaataaaa ccaactttcc atacagcaga aaatacaatt aaaagtatag
     2521 aatgtaatca cgaattatat aaaaagtatt ggagacctct tcatatatag ataattcact
     2581 tagttatttt agaaagaagc ccctaacaac taaagttgaa aaatagagga acacagttgg
     2641 atgctacgca tcaactgcat aagagcccct aacaactaaa gtttaaaagt aggagcgcct
     2701 gcgctaagcg catgcataag agcccctaac aactaaagtt gtaaggggct ctaaaattta
     2761 ttttggttga ttgtcttctg gtttatctga agtcattgtt tttgttgtat tatcatttaa
     2821 tgaatcttgt gctttttctg tttttgtttc aacagatgtt gtcactttat cattttctac
     2881 ttttgtattt tttggttcag tgacaacttt tttattcttg tcagctgttt ttactttatc
     2941 tttagtaatt ttttgaccac gttttaagta atattgaacg atacccatta aaatgattgc
     3001 atgaataaca acgaataaaa taattgtcat tactgtataa actcttatga tattttctgc
     3061 aacactttga gagagtgatt gcgtattaaa tgaaatcaag taaccaacag gtgttttaaa
     3121 taaaataata attaagttta ataatagtat tccaatgaaa aatttgaaaa atgttttttg
     3181 accatttttc attgctttaa atccattagc taagtgtgtt tttactttat cgttagttga
     3241 ttcaacgaaa ctagtcataa agttgaaaat aggtattaac aataaccaag taataattgc
     3301 tttaatcaat aaaacaacaa tgattatgat gctttgagtt gtaatagcaa taccaatcag
     3361 gtgtgtacta tctgcatatg atgattgtac tgaattcata atcatttctg atactggaga
     3421 aaataatttg tatagtaatg atgtcagaag tgaaagaacg ataatcattg ctatagatac
     3481 aagaccgatc agaacgctct ttaaataatt acctttttta aatgcaataa ataaatcggt
     3541 aaattttact ttttcatgac tcattgctcg tttcataaca tttgtaattc cgataaaaat
     3601 ttgaactaaa acaaacagtg aaactacagc tgctataagt aatacagcga taacttttaa
     3661 atatgcatct actggtggtt tttgcccaaa ttgtgaataa attgcaatag tttgtgcata
     3721 cttagcaagc tggaaattag caagtaaata tacaactgca attacagcaa atgctataag
     3781 tgcatatatc aatgttatta ataattgtgg tttagcattt ttaaatgctg atttaaagta
     3841 agtaaacaaa gtggtgcctc cttttcttca tataaataca ataacaaaaa acacgttatt
     3901 ttcatattaa tatctaataa aaattaatta aagtgactaa agttagagaa ttataatagt
     3961 aaattgtcaa aaatatgact aatgtaagaa ataatagttg aaaatttgtt aataaacatt
     4021 gcatcatcaa agaccaaaca agtcgccata aagacataaa aaacctactg ccattgaaaa
     4081 gtttaatcga tgcaatggca gtaggttaaa attattaatg tacatgcgcg tgatgatgag
     4141 catgatggtt atgtgaatga ctatgtgttc ctgaacatat aatcgattca tcatgtttgt
     4201 gattaggcgt ttctaattga atagtcatat ggtgaatatt taaatgtaat aaatcatgct
     4261 caatgttttc taataataat tcacattctt tcattgtcaa tgtatggtct acaacaacat
     4321 gacaactcaa tgcattcata tcatttgaaa ttgtccaaac atggcaatca tgcacacttt
     4381 gtattcgtga atccttttta atagtagtta taacttcatc tatatcaaca tcacttggtg
     4441 tgccttccat taaaatgtta attgaagatt ttgtgatacc ccaagcactt tttaaaataa
     4501 taacggaaac taaaatactt gcgataggat cggcgattgt ccatccaaat gcccaaatta
     4561 aaatagctgc agtaatggcg ccaactgaac ctaataagtc tccgataaca tgtagaaaag
     4621 caccacgcat atttaaattg tgtgaagtgt cgccgccttt aaacataaag aatgcaacaa
     4681 cgatattgac aattaaaccg ataatactaa tgattaacat ttcttttgat tgaacttcag
     4741 aaggaacaaa gaaacgttta atcgcttcaa aaacaatcaa aatacttatt acaaaaagcg
     4801 ttacaccgtt aaataacgct gcgagtactt cgaaacgttt ataaccaaat gtttttgtag
     4861 ttgtggcatt cttttcagca taaataaatg cgacaagtgc aacacctaat gagaatgtgt
     4921 cgctaaacat atggataccg tcagatagta atgccaagct gttagcaagg agaccgccga
     4981 tgatttcgat aaacatatat agaccgatta ttaaaaacga tataaacaat actttcttat
     5041 tatttgtagt tacatgacta tgcatatggt catgatgatg atgtgaatga gacatattat
     5101 aacccacttt ctttaggatg attcgcgtga tgtatggctt gctttaacat agttgctacg
     5161 tggatgtcat ctaatgaata aatcattgat tggccttgtc gttttgcttt cacaagatgc
     5221 acacttttaa gtaattttaa ttggtgcgag acatttgatt gagataaatt caattgatgt
     5281 gaaatgtgac caacacttgc ttcgctgact gataacaatt ccatgatacg tattcgattg
     5341 taatcgccta atgccttgaa aatttcagtt acgcgttcta atgtatctgt atttatttct
     5401 gaatattgtt ctgacattat atgacaatcc tttcatatga atatttgttc atatattaat
     5461 ttaatacgtt cccagtagta tgtcaattga tttttatagt tgtaacaagt tttacatatc
     5521 tcataaagtg gtatggcata gagaataata aataggagag tgttgtaaat ggatattttg
     5581 gttataggtg ctaatggcgg tgtaggttcg ttactagtgc aacaattagc aaaagaaaat
     5641 gtaccattta ctgctggtgt taggcaatca gatcaactta atgcgttaaa atcacaaggt
     5701 atgaaagcaa ttcttgttga tgttgaaaat gattcaatag agactttaac tgagacgttt
     5761 aaaccattcg ataaagttat cttttcagta ggttctggag ggaacacggg tgcagataaa
     5821 acaattattg tcgatttaga tggtgctgtt aaatcaatga ttgcgagtaa agaggccaat
     5881 attaaacact atgtcatggt ttcaacatat gattcgagac gtcaggcttt tgatgatagc
     5941 ggtgatttaa agccatatac aatagctaaa cattacgctg atgactattt aagacgttca
     6001 ggtttgaatt atacaatttt acatccaggg gcacttacaa atgcagcagg atctggaaag
     6061 atagaagctg cacaatattt tgatggtaaa ggtgaaattc ctagagaaga tgttgcgaca
     6121 gtattaaaag aaattgtcac atccaatcat tttaatcacc aagagtttca aattattagt
     6181 ggtgatcagg acattaaaga tgctttaact caatttgaaa atgaaacgga ttaataagga
     6241 tagtaaatat gatttgaagc gggttacttt ttcgtaactc gttatttttt gaaaatgatg
     6301 aattataagt atgaataagt acgtattgtc tatgcgtttt acaaagaatt tgaacaatgt
     6361 atggcacaac tattaaagtt gtttattaat tggtataatg ggcgagtaaa tttaaaggga
     6421 aatatgtaca aatgtaagag agatataatc tctatgctat aaatttttga aaatgttaaa
     6481 gtactgtaaa tttaagtgaa gcgctttaat tggcagtact gcaatgttag taaatatggt
     6541 acaatcacta atagttaatt tgaaatgtgg aagttaataa tacgatgtaa atgagaacaa
     6601 aatcaaaata taatggaaca acaacatcag agttctattg cttacttaat cacaatggag
     6661 gtgcaacaat gccattattt ttacaaccaa ttttaaaaac aaaattatgg ggcggtcaac
     6721 gtctaagtga gtttggatat caattagaca atgatacaac tggggaatgt tggtgtgtgt
     6781 cagcacatcc aaatggtacg agcgagatta ttaatggacc atatcaaggt caaacattag
     6841 accgtatttg gtcagaacat cgtgaattgt ttggtgattt cccaagcaaa gattttccgc
     6901 ttctaactaa aatagtggat gcaagagaat cactttctat tcatgtgcac cctgataatt
     6961 cttatgctta tgagcatgaa aacgggcaat atggcaaatc tgaatgttgg tatattatag
     7021 atgcagaaga agatgcagaa atagttatag ggacattagc agagtctaga gaagaagttg
     7081 cgaatcatgt tcaacacgga acgatagagt cgatacttag atatattaaa gtaaaacctg
     7141 gagaattcta ttttattcca gcaggaacag tacatactat ttcttcagga atattagcat
     7201 acgaaacgat gcaatcgtca gacattacat atagacttta tgatttcaat cgtcaagata
     7261 atcaatataa tgatagaccg ttaaatattg aaaaagcttt agacgttatt cagtacaatg
     7321 caccattacc taatattttg cctgaaagcg aaattattga aaaccataag tgtacacaca
     7381 ttgtatcgaa tgatttcttt acattggtta aatgggaaat ttctggcacg ttaaattata
     7441 tgaagcctag agagttctgt ttagttacag tgttggaagg cgaagggcaa atgattgtcg
     7501 atggtgaaat tttcaaactg actactggta caaactttat tttgacttct gaagatttgg
     7561 atagtgtctt tgaaggtgat ttcacattga tgattagcta tgtgtaatag tgtaataaca
     7621 atttaggagt gaatgatttg aagcaatttc tatatattgc gttagtatgt ggtgtgatag
     7681 caggtcttgg tgctttctta catataccgc agtatccgag catgacaatt ccacgtatag
     7741 tagctatttt aggaattatc agtgctatgt tgacttttaa agacaagcaa atcagcgcct
     7801 cattaaagtt tagcgcattg ttaattaatg tgctgccatt atgcggtacc tttgtagctt
     7861 caaattaatt gtcgatagcg cgtttgaata taatacagtg cattaacaga agaaatatga
     7921 ttgtgttaat gacattgatg aaaataaagc acatcaacat aacctctcac ttgcatagta
     7981 gtgagggggt ttttgttggt ttatataaaa t
//
LOCUS       DAXWFK010000114         7922 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.114, whole genome shotgun sequence.
ACCESSION   DAXWFK010000114 DAXWFK010000000
VERSION     DAXWFK010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7922
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.114"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(254..580)
                     /locus_tag="ACGEXS_001942"
     CDS             complement(254..580)
                     /locus_tag="ACGEXS_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HFF8012939.1"
                     /translation="MILDNVNPNDLFPTEKKGPSVLGIIEYQVQGENEFEGAFIATNE
                     RLIMNVDMNGQFYYRSISYNEIEKIDYDGQTIMFKFNIGNVPMHDIKSANVEMFVEYV
                     KQHMIV"
     gene            complement(644..1387)
                     /locus_tag="ACGEXS_001943"
     CDS             complement(644..1387)
                     /locus_tag="ACGEXS_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SarA family transcriptional regulator"
                     /protein_id="HFF8012940.1"
                     /translation="MSKFKVKRLTAHILMLKVINDMLKYSFHLTLTQVKLLNLLVKYD
                     NSDSNSASIDSLLKMKEIQSKMALLQMLSYLNNHQWLLKGRDQRDQRKLTISINKMHI
                     DKIEYMNNELSDYIERYFGQYTFDFSCDYVSYLLSSQELLRNLKCYLNMCQLSLEELY
                     VLGILNLHKGQLTVKELQGEFHHPIFAVSLILKCLILKGLVKKERCELDERRVIVTIK
                     REKFSKVTMLIQACYNYLEKGIQVKLNNK"
     gene            complement(1413..3368)
                     /locus_tag="ACGEXS_001944"
     CDS             complement(1413..3368)
                     /locus_tag="ACGEXS_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8012941.1"
                     /translation="MTDNYSLHIYKSMKMSSVKEANLKIIYWLQGTGVVSINLQQYDV
                     KRNDVTIIMLNDLYQIDGNDDSVCCVVEVSAKTFLKFMNANYMMRGKILTNEVASTFR
                     ILIKYLIYSKIKQQSYNANDKIINYMCVELMSLNTYDGSRHLVAEEVHDYLTNNHHKK
                     INRKDVINQVSITNKALSDMFKATPYSNFIQYLNHIRLEHCLIDILTSKKPIEEIASS
                     HGFNHYSRFIHLFKETYGNTPKLIRKRYSPTSHSINHSQLVEIDKNIIELFDDTNQSS
                     SSMREIDIPFEPTKRSQMYQPKNIYIKGSNFYWMDYYTIKQIIQRLGVNPENLHIIVT
                     IDTSNELSIEQITLLLQKIVNYNVNVVFRIKHEYKELLTSAERGKIEKLVATIFNMTF
                     ESNRCKIAFLINDLNTTAIQKLKRLIDNYIGVFELIYHLEPKELNTRYDKEIDHLIDY
                     FILPLRQIEQVSIARSKIICDVDLINENNDLKEKITMSDINKVSELLTNYTKFRGLIM
                     HMSIISESSKEINLTRLSIFIFIINILNQLRGIVIYQNDTMIVTKFKHEVQCVICLPA
                     ALLNENSDAIRLSCKGLQQFSHAKTKQIVLSIDKDNHTSENGVLVNDIMQQHSYWKAN
                     FIERNIYDHSLMVSSNSIVHLKYHLEI"
     gene            3978..4781
                     /locus_tag="ACGEXS_001945"
     CDS             3978..4781
                     /locus_tag="ACGEXS_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HFF8012942.1"
                     /translation="MKKIATATIATAGFATIAIASGNQAHASEQDNYGYNPNDPTSYS
                     YTYTIDAQGNYHYTWKGNWHPSQLNQDNGYYSYYYYNGYNNYNNYNNGYSYNNYSRYN
                     NYSNNNQSYNYNNYNSYNTNSYRTGGLGASYSTSSNNVQVTTTMAPSSNGRSISSGYT
                     SGRNLYTSGQCTYYVFDRVGGKIGSTWGNASNWANAAARAGYTVNNTPKAGAIMQTTQ
                     GAYGHVAYVESVNSNGSVRVSEMNYGYGPGVVTSRTISASQAAGYNFIH"
     gene            complement(4798..5040)
                     /locus_tag="ACGEXS_001946"
     CDS             complement(4798..5040)
                     /locus_tag="ACGEXS_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012943.1"
                     /translation="MTKLIENLKASIFVSTLTKNLSMYSRFDMPKFGCVNGCMLFILY
                     YRVNRIGENRNFEIFTNSIFFIEIKKANKALETLLA"
     gene            complement(5214..6614)
                     /gene="nhaC"
                     /locus_tag="ACGEXS_001947"
     CDS             complement(5214..6614)
                     /gene="nhaC"
                     /locus_tag="ACGEXS_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFF8012944.1"
                     /translation="MMKRKPTFLESISTMIVMVIVVVTGFVFFDIPIQVLLIIASAYA
                     TWIAKRVGLTWQDLEKGIAERLNTAMPAILIILAVGIIVGSWMFSGTVPALIYYGLDL
                     LNPSYFLISAFFISAVTSVATGTAWGSASTAGIALISIGNQLGIPPGMAAGAIIAGAV
                     FGDKMSPLSDTTNLAALVTKVNIFKHIHSMMWTTIPVSIIGLLVWFIAGFQFKGHSND
                     KQIQTLLSELAQIYQINIWVWVPLIVIIVCLLFKMATVPAMLISSFSAIIVGTFNHHF
                     KMTDGFKATFSGFNESMIHQSHISSSVKSLLEQGGMMSMTQILVTIFCGYAFAGIVEK
                     AGCLEVLLTTISKGIHSVGSLICITVICCIALVFAAGVASIVIIMVGVLMKDLFEKYQ
                     VSRSVLSRTLEDSSTMVLPLIPWGTSGIYYTNQLHVSVEEFFIWTVPCYLCAIIAIIY
                     GFTGIGIKKSSNSRLT"
     gene            complement(6709..7791)
                     /locus_tag="ACGEXS_001948"
     CDS             complement(6709..7791)
                     /locus_tag="ACGEXS_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HFF8012945.1"
                     /translation="MKIAIVGSGNGAVTAAVDMVSKGHDVKLYCRNQSISKFQNAIEK
                     GGFDFNNEGDERFVKFTDISDDMEYVLKDAEIVQVIIPSSYIEYYADVMAEHVTDNQL
                     IFFNMAAAMGSIRFMNVLEDRHIETKPQLAEANTLTYGTRVDFENAAVDLSLNVRRIF
                     FSTYDRSCLNDCYDKVSSIYDHLVKEESLIKTNLENGNPEVHPGPTLLNVGRIDYAGE
                     FALYKEGITKHTVRLLHAIELERLNLGRRLGFELSTAKESRIERGYLERDKEDEPLNR
                     LFNTSPVFSQIPGPNHVESRYLTEDIAYGLVLWSSLGRVIDVPTPNIDAVIVIASTIL
                     ERDFFEEGLTVEEIGLDKLDLEKYLK"
BASE COUNT         2724 a         1432 c         1002 g         2764 t
ORIGIN      
        1 atgtttaaga tattttgcca aattgaaaat tattagtctg aatattttgt atcttaactg
       61 atatgtaaac ttttaaaatt ttaatctata tcaacgtgtt tgatttgata ttttttatat
      121 taattggatg gcttgctcac aaaatttata aaatataaaa aaagaagagc tttaaatcat
      181 aacgcatgcc atttatcttt aaatgcaatg agttatcatt caaactcttc ttatcaacct
      241 acatacattg acattatact atcatatgtt gttttacata ttcaacaaac atttcaacgt
      301 tagcactttt aatatcatgc attgggacat taccaatatt aaatttgaac attatagttt
      361 gaccatcata atctatcttc tctatttcat tataactaat acttctataa taaaattggc
      421 cattcatatc tacattcata attaatcttt catttgttgc tataaatgcc ccttcaaatt
      481 cattctcacc ttgaacttga tactcaatta ttcctagcac tgacggaccc tttttttcag
      541 ttggaaataa atcatttgga ttcacattat ctaaaatcat aatatccctc ccacttaaaa
      601 ctataaacta ttcttcatag gtatatgaaa tacaagtgat taactattta ttattaagct
      661 taacttgtat tcccttttca agataattat aacatgcttg aataagcata gtgactttag
      721 aaaacttttc tcgcttgatt gttacaataa cacgcctttc atctaattca catctctctt
      781 ttttaactaa tcccttcaaa attaaacact ttaaaatcag gctaaccgca aatatcgggt
      841 gatggaattc accctgtaat tctttaaccg tcaattgacc tttatgcaga tttaatattc
      901 ctaaaacgta taactcttct aatgataatt gacacatatt taaataacac tttagattcc
      961 gcaataattc ttgtgacgac aataaatacg atacatagtc acaactaaaa tcaaatgtat
     1021 attgaccaaa ataacgttca atatagtcac taagctcatt attcatatat tctattttat
     1081 ctatatgcat tttattaatc gaaatagtca atttacgttg atccctttga tctcttcctt
     1141 tcaatagcca ttgatgattg tttaaataac ttaacatttg aagaagggcc atcttagatt
     1201 ggatttcttt catttttaat aaactatcta ttgaagcgct attggaatca gaattatcat
     1261 atttaactaa tagatttaat agtttcactt gagtcaaagt gagatgaaat gaatatttca
     1321 acatatcatt aatgactttc aacattagta tgtgtgcagt taaacgctta actttaaatt
     1381 tagacatctt taaaacctct cttaaaccat gcctatatct caagatgata tttcaaatga
     1441 acaatactat tgcttgagac cattaatgaa tgatcataaa tatttctttc tataaaatta
     1501 gctttccaat aactgtgttg ttgcataata tcattcacaa gtacaccatt ttcggaagta
     1561 tgattatctt tatctatact taaaacaatt tgtttagttt tagcatggct aaattgttga
     1621 agacccttac acgataaacg tatagcgtct gaattctcat ttaacaatgc ggctggcaaa
     1681 caaatgacac attgtacttc atgtttgaac ttcgttacaa tcatcgtgtc attttgataa
     1741 ataacaatcc ctcgtaattg attaagtata tttatgataa aaatgaatat tgataatcta
     1801 gttaaattta tttctttgct cgattcactt ataattgaca tgtgcataat aagtcctcga
     1861 aattttgtat aatttgtcaa aagttcagaa accttattaa tatcagacat tgtgattttt
     1921 tcttttaagt cattattctc attaatcaaa tctacatcac atataatttt agaacgtgca
     1981 atagacacct gttctatttg tcttagcggt aagataaaat agtctattag atgatcaatt
     2041 tctttatcat agcgcgtatt caactctttt ggttccagat gatatattaa ttcaaatact
     2101 cctatataat tatctattaa acgtttcagt ttttgaattg cagtagtatt taagtcgtta
     2161 attaaaaatg caatcttaca tctattactc tcaaaagtca tattaaaaat tgtagctact
     2221 agcttttcta tcttgcctct ttcagcactc gttaacaatt ccttatattc atgtttaata
     2281 cgaaacacta catttacatt gtaattcact atcttttgaa gtaataaagt gatttgttca
     2341 atcgatagct cattgcttgt gtcgattgta acaattatat gcaagttttc aggattaaca
     2401 cctaatcttt gaatgatttg tttaatagta taataatcca tccaataaaa attacttcct
     2461 ttaatataaa tgtttttagg ttgatacatt tgactccttt ttgtaggctc aaaaggtata
     2521 tcaatctcgc gcatacttga agaactttga ttagtatcat caaataattc aattatattt
     2581 ttatcaattt caactaattg ggaatggtta attgaatgag atgttggact atatcttttt
     2641 ctaattaatt taggagtgtt accatacgtt tctttaaaga ggtgtataaa acgagaataa
     2701 tgattaaaac catgactact tgcgatttct tcaataggct tcttagaagt taaaatatca
     2761 attaagcaat gctccagtct aatatgattt aaatattgaa taaaattact ataaggtgtc
     2821 gctttaaaca tgtcacttaa agctttgttt gtaatactaa cttgattaat gacatctttc
     2881 ctatttatct ttttatggtg gttgtttgtt aaataatcgt gcacttcttc ggctactaaa
     2941 tgacgactac catcgtatgt atttaatgac attaattcaa cacacatgta attaataatc
     3001 ttatcattag cattataaga ctgttgttta attttgctgt agattaaata cttaatcaag
     3061 attctaaaag tgctagcaac ctcatttgtt aatatcttgc cacgcatcat atagtttgca
     3121 ttcataaact tcaaaaatgt ctttgcactt acttcaacaa cacaacatac actatcatca
     3181 ttaccatcaa tttgatacaa atcattcaac ataataatgg tgacatcatt tctttttaca
     3241 tcatattgtt gtagattgat tgaaactaca cctgtacctt gtagccaata tataattttt
     3301 aagttcgctt ccttcacact actcattttc atacttttat aaatatgtag tgaataatta
     3361 tctgtcatct tacatcctca catatacaaa tatattgatt tactcctgtt tgtctttctc
     3421 aaatcaattg tcaacatcta atattcacta ccaattatat cgccacatgt tttttagcag
     3481 taacaaaaca taaatcgatt atatcaaata atacttttat taaaacattt ttttatgcta
     3541 aattaggata ttttatccat taaatataca ttttcatcca aataccaaag ctttcataat
     3601 catagaaact tgatcgttcg tgtcgaattt ttatgcattt tctgattaaa agtcctatat
     3661 tatgcacaaa atatacattt agaattttca gtaatattac aaatttgtaa cagacttatt
     3721 ttcgaaacat tcagttttca caaagaatgc cgtagttata agttttgcaa aatgcaaatt
     3781 tctatacttt taaaattcta ttgtgtcaag atattagcaa aaaattacaa aacgttaaca
     3841 aagcacacaa ggacgctaat ttctgttata gtctttcttg tcgtttaatt acgagagata
     3901 acttataaca ttaattttgt cacacggaca ttacgaaaat attttagatt taaaattttt
     3961 aggaggatat ttttaaaatg aagaaaatcg ctacagctac tatcgcaact gcaggattcg
     4021 ctacaatcgc aattgcatca ggaaatcaag ctcatgcttc tgagcaagat aactacggtt
     4081 ataatccaaa cgacccaaca tcatatagct atacttacac tattgatgca caaggtaact
     4141 accattacac atggaaaggt aactggcatc caagtcaatt aaaccaagat aatggctact
     4201 acagctatta ctactacaat ggttacaata actataacaa ttacaacaac ggttatagct
     4261 acaataacta cagccgttac aacaactact caaataataa tcaatcatat aactacaata
     4321 actataatag ttacaacaca aacagctacc gtactggtgg tttaggtgca agctacagca
     4381 cttcaagcaa caatgttcaa gtaactacaa ctatggctcc atcatcaaat ggccgttcaa
     4441 tctcaagtgg ttatacttca ggacgtaact tatacacttc tggtcaatgt acatactacg
     4501 tatttgatcg tgtaggtggt aaaatcggtt caacttgggg caatgcaagt aactgggcta
     4561 acgcagctgc aagagctggt tacacagtga acaatacacc aaaagctggt gcaattatgc
     4621 aaacaactca aggtgcatac ggtcacgttg catacgttga aagtgttaac agcaatggtt
     4681 cagtaagagt ttcagaaatg aactatggtt atggcccagg tgttgtaact tcacgtacaa
     4741 tctcagctag ccaagctgct ggttataact tcattcacta attagtgatg gctatgttta
     4801 cgccaacaag gtttcaagag ccttgttggc ttttttaatt tctatgaaaa aaatcgaatt
     4861 ggtaaaaatc tcaaaatttc gattttcacc aatccgattc actctgtaat aaagaataaa
     4921 caacatacag ccattgacac acccaaattt aggcatgtca aatcggctat acattgataa
     4981 atttttagtt aaagtactta caaatatact tgccttcaaa ttctcaatta atttagtcat
     5041 tacaaatcat gacataaaac ttacattaca tatatcaaca tgttccgcta caaaacatat
     5101 tcagtatatg tatagaggat atttattcta agttaaaacc ttttcaaatt ataaccgcta
     5161 caattataaa tgattaaagt gtttcaacat attatatata ttccacgctc acattaagtt
     5221 aaacgtgaat tcgatgactt tttaatacct atccctgtaa aaccatagat aattgctata
     5281 attgcgcata aataacatgg tactgtccat atgaaaaatt cttcaacaga gacatgaagt
     5341 tgattcgtat agtaaatacc tgatgttccc catggtatta aaggtaagac catcgtactt
     5401 gagtcttcaa gtgttcttga taaaacggaa cgagagactt ggtatttttc gaacaaatct
     5461 ttcattaaca caccgaccat aataattaca atcgaagcaa cacctgcagc gaatacaagc
     5521 gcaatacaac aaataacagt aatacatatt aaacttccta cagaatggat gcctttagaa
     5581 atagtagtta ataagacttc taaacatcct gctttttcta caatacctgc aaatgcatat
     5641 ccgcaaaata tcgttactaa tatttgggtc atactcatca taccaccctg ttctaacaag
     5701 cttttcacac tggatgaaat atgagactga tgtatcattg attcgttaaa accactaaat
     5761 gttgctttga aaccatctgt cattttgaaa tgatgattaa aagtccccac tataatggca
     5821 gaaaagcttg atattagcat agctggcact gtagccattt taaatagcaa acaaacaatg
     5881 atcacaatta agggaaccca gacccatatg ttaatttgat aaatctgtgc aagctctgat
     5941 aacaaagttt gaatctgttt atcatttgaa tgccctttaa attgaaatcc agcaataaac
     6001 catactaata aacctatgat tgatacaggt atcgtcgtcc acatcatcga atgtatatgt
     6061 ttaaatatat taactttagt aacaagcgcc gctaaattag ttgtatctga taatggtgac
     6121 attttatcgc caaacacagc ccctgctata atagcacccg ctgccatccc tggaggaatc
     6181 cccaattgat taccaataga aataagtgca atccctgcag ttgatgcaga gccccatgct
     6241 gtaccagttg ctacagatgt aacagcactt ataaaaaagg ctgatattaa aaaatagctt
     6301 ggattcaata aatctaagcc ataataaatc aaggctggca ctgtgccaga aaacatccaa
     6361 ctgcctacta taattcctac cgctagtata attaaaattg caggcattgc agtatttaaa
     6421 cgttctgcaa tgcctttttc taaatcttgc catgttaagc ctacacgttt tgcaatccat
     6481 gtggcatatg ctgaggcaat aattaataat acttgaattg gaatatcaaa aaacacaaag
     6541 cctgttacaa caacaataac cattacaatc attgtcgaaa ttgattctaa aaaggtgggt
     6601 tttcttttca tcatctatcg tccccttcgc ttattgatga gaaacctctt ttcttcaatt
     6661 tattgctttt gcataatgct atatcctttt ctatcttcaa gccatcattt attttaaata
     6721 tttttctaaa tcaagcttat ctaaaccaat ttcttcaact gttaagcctt cctcaaagaa
     6781 gtctctctct aaaatggttg atgcaattac aattactgca tctatatttg gtgtcggtac
     6841 atcaataaca cgacctaagc ttgaccatag tactaaacca tatgcaatat cttcagttaa
     6901 atatctgctt tctacatgat ttggtcctgg aatttgtgaa aatactgggc ttgtattaaa
     6961 caaacgattt aatggttcat cttctttatc acgttctaaa taaccacgtt caatacgtga
     7021 ttctttagct gttgataatt caaaacctaa tctacggcct aaattcaaac gttctaattc
     7081 gattgcatga agtaatctaa ctgtatgttt agtaattcct tctttatata aagcgaactc
     7141 gccagcataa tcaatgcgac cgacattcaa taatgttggt cctggatgca cttcaggatt
     7201 accattttca agatttgttt taattaagct ttcctctttt actaaatgat cataaatact
     7261 tgaaactttg tcataacaat catttagaca gcttctatca tatgttgaaa agaagatacg
     7321 acgtacattt agagataaat caactgctgc attttcaaaa tcgacacgcg taccatacgt
     7381 caacgtatta gcttccgcta gttgtggttt tgtttcaata tgtctatctt ctaaaacatt
     7441 cataaaacga attgacccca ttgctgcagc catgttgaag aatatcaact gattatcagt
     7501 tacatgctct gccattacat cagcataata ctctatgtat gaagatggaa taatcacttg
     7561 aacaatttca gcatctttta aaacatattc catatcatca ctaatatcag tgaattttac
     7621 gaaacgttca tcaccttcat tattaaaatc aaatccgccc ttttcgattg cgttttgaaa
     7681 cttacttata gattgattac gacaatataa tttaacatcg tggcctttgc tcaccatatc
     7741 tactgctgcc gtaactgcgc catttcctga tcctacaatt gctattttca taaacaaccc
     7801 tctttctatc tttggttaaa attaaaaaca aattatacgt aagacaatat aaaaatgatg
     7861 cgaattcatt acgcatcatt ataaatgtta agtatatata accttaaatt aacctattta
     7921 aa
//
LOCUS       DAXWFK010000115         7907 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.115, whole genome shotgun sequence.
ACCESSION   DAXWFK010000115 DAXWFK010000000
VERSION     DAXWFK010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7907
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.115"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(411..770)
                     /locus_tag="ACGEXS_001949"
     CDS             complement(411..770)
                     /locus_tag="ACGEXS_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFF8012946.1"
                     /translation="MNIISTILIIFVALEFFYIMYLETIAITSKKTSETFNISVDKLK
                     DKNINLLLKNQGVYNGLIGVLLIYGLFISSNPKEICAAILVYIIGVAIYGGLSSNISI
                     FFKQGTLPVLALISMLW"
     gene            complement(789..1634)
                     /locus_tag="ACGEXS_001950"
     CDS             complement(789..1634)
                     /locus_tag="ACGEXS_001950"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFF8012947.1"
                     /translation="MNIMLTGATGHLGTHITNQAIANHIDHFHIGVRNVEKVPEDWRG
                     KVPVRQLDYFNQESMVEAFKGMDTVVFIPSIIHPSFKRIPEVENLVYAAKQSGVAHII
                     FIGYYADQHNNPFHMSPYFGYAARLLATSGIDYTYVRMAMYMDPLKPYLPELMNMHKL
                     IYPAGDGRINYITRNDIARGVIAIIKNPDTWGKRYLLSGYSYDMKELAAILSEASGTE
                     IKYEPVSLETFAEMYDEPKGFGALLASMYDAGARGLLDQESNDFQQLVNDQPQTLQSF
                     LQENI"
     gene            2106..2786
                     /locus_tag="ACGEXS_001951"
     CDS             2106..2786
                     /locus_tag="ACGEXS_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL1"
                     /protein_id="HFF8012948.1"
                     /translation="MKFKAIAKASLALGMLATGVITSNVQSVQAKAEVKQQSESELKH
                     YYNKPILERKNVTGFKYTDEGKHYLEVTVGQQHSRITLLGSDKDKFKDGENSNIDVFI
                     LREGDSRQATNYSIGGVTKSNSVQYIDYINTPILEIKKDNEDVLKDFYYISKEDISLK
                     ELDYRLRERAIKQHGLYSNGLKQGQITITMNDGTTHTIDLSQKLEKERMGESIDGTKI
                     NKILVEMK"
     gene            3072..3767
                     /locus_tag="ACGEXS_001952"
     CDS             3072..3767
                     /locus_tag="ACGEXS_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL2"
                     /protein_id="HFF8012949.1"
                     /translation="MKMKNIAKISLLLGILATGVNTTTEKPVHAEKKPIVISENSKKL
                     KAYYNQPSIEYKNVTGYISFIQPSIKFMNIIDGNSVNNIALIGKDKQHYHTGVHRNLN
                     IFYVNEDKRFEGAKYSIGGITSANDKAVDLIAEARVIKEDHTGEYDYDFFPFKIDKEA
                     MSLKEIDFKLRKYLIDNYGLYGEMSTGKITVKKKYYGKYTFELDKKLQEDRMSDVINV
                     TDIDRIEIKVIKA"
     gene            4058..5128
                     /locus_tag="ACGEXS_001953"
     CDS             4058..5128
                     /locus_tag="ACGEXS_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL3"
                     /protein_id="HFF8012950.1"
                     /translation="MKMRTIAKTSLALGLLTTGAITVTTQSVKAEKIQSTKVDKVPTL
                     KAERLAMINITAGANSATTQAANTRQERTPKLEKAPNTNEEKTSASKIEKISQPKQEE
                     QKTLNISATPAPKQEQSQTTTESTTPKTKVTTPPSTNTPQPMQSTKSDTPQSPTIKQA
                     QTDMTPKYEDLRAYYTKPSFEFEKQFGFMLKPWTTVRFMNVIPNRFIYKIALVGKDEK
                     KYKDGPYDNIDVFIVLEDNKYQLKKYSVGGITKTNSKKVNHKVELSITKKDNQGMISR
                     DVSEYMITKEEISLKELDFKLRKQLIEKHNLYGNMGSGTIVIKMKNGGKYTFELHKKL
                     QEHRMADVIDGTNIDNIEVNIK"
     gene            5247..5384
                     /locus_tag="ACGEXS_001954"
     CDS             5247..5384
                     /locus_tag="ACGEXS_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012951.1"
                     /translation="MFVKVAFLCLKSDETSNVPSVESHQNHFYLTNIMDFLIYLTMIQ
                     I"
     gene            5493..6455
                     /locus_tag="ACGEXS_001955"
     CDS             5493..6455
                     /locus_tag="ACGEXS_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL4"
                     /protein_id="HFF8012952.1"
                     /translation="MKITTIAKTSLALGLLTTGVITTTTQAANATTPSSTKVEAPQST
                     PPSTKIEAPQSTPPSTKIEAPQSKPNATTPPSTKVEAPQQTANATTPPSTKVTTPPST
                     NTPQPMQSTKSDTPQSPTTKQVPTEINPKFKDLRAYYTKPSLEFKNEIGIILKKWTTI
                     RFMNVVPDYFIYKIALVGKDDKKYGEGVHRNVDVFVVLEENNYNLEKYSVGGITKSNS
                     KKVDHKAGVRITKEDNKGTISHDVSEFKITKEQISLKELDFKLRKQLIEKNNLYGNVG
                     SGKIVIKMKNGGKYTFELHKKLQENRMADVIDGTNIDNIEVNIK"
     gene            6819..7523
                     /locus_tag="ACGEXS_001956"
     CDS             6819..7523
                     /locus_tag="ACGEXS_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL5"
                     /protein_id="HFF8012953.1"
                     /translation="MKMTAIAKASLALGILATGTITSLHQTVNASEHKAKYENVTKDI
                     FDLRDYYSGASKELKNVTGYRYSKGGKHYLIFDKNRKFTRVQIFGKDIERFKARKNPG
                     LDIFVVKEAENRNGTVFSYGGVTKKNQDAYYDYINAPRFQIKRDEGDGIATYGRVHYI
                     YKEEISLKELDFKLRQYLIQNFDLYKKFPKDSKIKVIMKDGGYYTFELNKKLQTNRMS
                     DVIDGRNIEKIEANIR"
BASE COUNT         3067 a         1206 c         1327 g         2307 t
ORIGIN      
        1 tgtaatgcca atcaacaccg aagttctcat gtcctctttc cttttacgac tacatatatt
       61 gttaataata ttgaaataat aaactgatag tgataatatt tatttaactg atccatgaga
      121 gtaggaaccc catcatacgg gaatttcgaa atcatgggtt ttttgtttta aaggattgat
      181 gcaatagata aagtagccat tctaatatat ggagtattta tcagctgaaa attaaatcag
      241 atatgggttt tcttcattaa ataatttttc tataaggaaa cagaggcaac gctactgtca
      301 cctctgcttg aaataattag gatgaaatac tctgagtcta aagtccacac acaattcgct
      361 caaatcctcc aaacctccga ctacatctcc aaccccccaa acaccaatac ttaccaaagc
      421 attgatatga gtgccaatac tggcaatgtg ccttgtttga aaaagatact aatattgctt
      481 gaaaggccac cataaatagc aacgccaatg atatacacta aaatagctgc gcatatttct
      541 tttggattac tgctgataaa caaaccgtat attagcaaaa ctccgattaa accgttatat
      601 acgccttggt tcttcaaaag taggttaata tttttgtctt tcaatttatc gacgcttata
      661 ttaaatgtct cgctagtctt tttggaagtt atagcaatcg tttcaaggta cataatatag
      721 aaaaactcta atgccacaaa tatgattaaa attgttgaga tgatattcac tataacgctc
      781 ctttattatt aaatattttc ttgtaaaaat gattgcagtg tctgtggttg atcattgact
      841 aattgttgga aatcattgga ttcttggtct aatagtcctc ttgctcctgc gtcgtacatt
      901 gatgccaata atgcaccaaa gcctttaggt tcatcataca tttctgcaaa tgtctctaat
      961 gaaacgggct catatttaat ttctgtgcct gatgcctcag ataaaattgc agcaagttct
     1021 ttcatatcat aactgtagcc tgataataag tagcgtttgc cccaagtatc tggattttta
     1081 ataatagcaa tgacacctct agcaatatca tttctagtaa tataattaat acgaccatcg
     1141 ccagctggat aaatcagttt atgcatattc atcaattctg gtaaatatgg tttaagtgga
     1201 tccatgtaca ttgccattct tacatacgta tagtcaatgc cacttgttgc caatagacgt
     1261 gctgcataac caaaataagg actcatatgg aatggattat tatgctgatc tgcgtaataa
     1321 cctatgaaaa tgatatgagc aacgccgctc tgctttgccg catatactaa attttccact
     1381 tcaggaatac gtttgaatga tggatggata atacttggaa taaacacaac ggtatccatt
     1441 cctttaaatg cttctaccat gctttcttga ttaaaataat ctaattgtcg aacaggaact
     1501 tttccgcgcc aatcttctgg aactttctca acatttctaa caccaatgtg aaaatgatct
     1561 atgtgatttg caatggcttg atttgtaata tgtgtgccta aatgacctgt agcacctgtt
     1621 aacataatat tcattcactt catctcctaa tctttatata cataacataa tacttatttg
     1681 atggttttca aaacatttga ttttataaaa aattctaatc tgtatttatt gtcgacgtgt
     1741 atagtaaata cgtaaatatt attaatgttg aaaatgccgt aatgacgcgt tttagttgat
     1801 gtgtatcact aatatcattg aaaattttaa tcaggtacta cgacaatatg atgtctgttt
     1861 tgtgtctgaa agttttacag tttttaaaat aaaaatggta taaagtgtga tttgtataaa
     1921 aaagagtctc gacggataag aattgattaa taacagttag cattttatta attaccttaa
     1981 caatgattca agtttagtta aatgaggttt aatttgaaag gggatagcgc ctcaatataa
     2041 tgtaggtaga ttgttcatat tacgtaattg aaaaatcaaa tttaaataga ttggggctaa
     2101 aaattatgaa atttaaagcg atagcaaaag caagtttagc attgggaatg ttagcaacag
     2161 gtgtaattac atcgaatgta caatcagtac aagcgaaagc agaagttaaa caacaaagtg
     2221 aatcagagtt aaaacactat tataataaac caattttaga gcgtaaaaat gtgactggat
     2281 ttaaatatac tgatgagggt aaacactatt tagaagtcac agtagggcaa cagcattctc
     2341 gaatcacttt acttggatct gataaagata aatttaaaga cggagaaaac tcaaatatag
     2401 atgtgtttat ccttagagaa ggtgacagta gacaagcaac aaattactca attggtggcg
     2461 ttacaaaatc aaatagtgtg cagtatattg attatatcaa tacgccaatt ttagaaatca
     2521 agaaagataa tgaagatgta cttaaagatt tttactacat ttcaaaagaa gacatctcat
     2581 taaaagaact tgattataga ttaagagaac gtgcgattaa acaacacggc ttgtattcaa
     2641 atggtcttaa acaaggtcaa attacaatta caatgaatga tggcacaaca catacaatcg
     2701 atttaagtca aaaacttgaa aaagaacgta tgggtgagtc aatcgacggc actaagatta
     2761 ataaaattct agtagaaatg aaataatact ttctaacaac aaagcgctat gttgaatagt
     2821 gcttgttatg gaaatatatg gaagttaagc gacgtactgt tgcttagctt ctttttttga
     2881 ggggaaaagt tacaaaactc acacaaacag tcgcaccacg cattatcttt tgcttaaata
     2941 gcttaatcat attttatgaa tagttaaaaa caggttaatg tgaatatccg aatacagctc
     3001 ctataatatg ggtgtatgat tcaaattacg taataaaaca atctaattat aatagattgg
     3061 agcatacaac tatgaaaatg aaaaatattg caaaaataag tttgttatta ggaatattag
     3121 caacaggtgt aaacactaca acggaaaaac cagttcatgc cgaaaagaaa cctattgtaa
     3181 taagtgaaaa tagcaaaaaa ttaaaagctt attataatca acctagtatt gaatataaaa
     3241 atgtgacagg ttatatcagt ttcattcaac caagtattaa atttatgaat atcatagatg
     3301 gtaattctgt taataatatt gctttaattg gcaaagataa gcaacattat catacgggtg
     3361 tacatcgtaa tcttaatata ttttacgtta atgaggataa gagatttgaa ggtgcaaagt
     3421 actctattgg gggtatcacg agtgcaaacg ataaagctgt cgacctaata gcagaagcaa
     3481 gagttattaa agaagatcat actggtgaat atgattatga ctttttccca tttaaaatag
     3541 ataaagaagc gatgtcattg aaagagattg attttaaatt aagaaaatac cttattgata
     3601 attatggtct ttacggtgaa atgagtacag gaaaaattac agtcaaaaag aaatactatg
     3661 gaaagtatac atttgaattg gataaaaagt tacaagaaga ccgtatgtcc gatgttatca
     3721 atgtcacaga tattgataga attgaaatca aagttataaa agcataacac atatacttga
     3781 tgacgaaata agttgaaatt gaaatagaga ggttaagtga cgatcaaacg ttgcttaact
     3841 tctttttaat gcttaaaaat tatttcaaag gcacatagaa acgctatatt aatctcatac
     3901 tcactcatta ttttttgctt aaattactta ataatacttc aataattgtt aaaaggggtt
     3961 taatgtgatt atcttagaac gccatctata atgatgttgt atgattcaaa ttacgtaaaa
     4021 agacaatcga atataatata gattggagca tacaattatg aaaatgagaa caattgctaa
     4081 aaccagttta gcactagggc ttttaacaac aggcgcaatt acagtaacga cgcaatcggt
     4141 caaagcagaa aaaatacaat caactaaagt tgacaaagta ccaacgctta aagcagagcg
     4201 attagcaatg ataaacataa cagcaggtgc aaattcagcg acaacacaag cagctaacac
     4261 aagacaagaa cgcacgccta aactcgaaaa ggcaccaaat actaatgagg aaaaaacctc
     4321 agcttccaaa atagaaaaaa tatcacaacc taaacaagaa gagcagaaaa cgcttaatat
     4381 atcagcaacg ccagcgccta aacaagaaca atcacaaacg acaaccgaat ccacaacgcc
     4441 gaaaactaaa gtgacaacac ctccatcaac aaacacgcca caaccaatgc aatctactaa
     4501 atcagacaca ccacaatctc caaccataaa acaagcacaa acagatatga ctcctaaata
     4561 tgaagattta agagcgtatt atacaaaacc gagttttgaa tttgaaaagc agtttggatt
     4621 tatgctcaaa ccatggacga cggttaggtt tatgaatgtt attccaaata ggttcatcta
     4681 taaaatagct ttagttggaa aagatgagaa aaaatataaa gatggacctt acgataatat
     4741 cgatgtattt atcgttttag aagacaataa atatcaattg aaaaaatatt ctgtcggtgg
     4801 catcacgaag actaatagta aaaaagttaa tcacaaagta gaattaagca ttactaaaaa
     4861 agataatcaa ggtatgattt cacgcgatgt ttcagaatac atgattacta aggaagagat
     4921 ttccttgaaa gagcttgatt ttaaattgag aaaacaactt attgaaaaac ataatcttta
     4981 cggtaacatg ggttcaggaa caatcgttat taaaatgaaa aacggtggga aatatacgtt
     5041 tgaattacac aaaaaactgc aagagcatcg tatggcagac gtcatagatg gcactaatat
     5101 tgataacatt gaagtgaata taaaataatc atgacattct ctaaatagaa gctgtcatcg
     5161 gaaaaacaag aagttaagtg acaacggttt acatgttgct tagcttcttt tattatgcgt
     5221 aatgatgtaa aaagacgaat attcatttgt ttgtaaaagt ggcatttcta tgtcttaaaa
     5281 gtgacgaaac ttcaaatgtg ccaagtgttg aatcacatca aaatcatttt tatttaacga
     5341 acattatgga tttcttaatt tacttaacga tgattcaaat atagttaaac aaggtttaat
     5401 gtgaatggag caatacgcca tctataataa agctgtatga ttcaatgaat gtaatcgaac
     5461 aaatctaata attacgaatg gagcatacaa ctatgaaaat aacaacgatt gctaaaacaa
     5521 gtttagcact aggcctttta acaacaggtg taatcacaac gacaacgcaa gcagcaaacg
     5581 cgacaacacc atcttccact aaagtggaag caccacaatc aacaccgccc tcaactaaaa
     5641 tagaagcacc acaatcaaca ccgccctcaa ctaaaataga agcaccgcaa tcaaaaccaa
     5701 acgcgacaac accgccctca actaaagtag aagcaccgca acaaacagca aatgcgacaa
     5761 caccgccttc aactaaagtg acaacacctc catcaacaaa cacgccacaa ccaatgcaat
     5821 ctactaaatc agacacacca caatcgccaa ccacaaaaca agtaccaaca gaaataaatc
     5881 ctaaatttaa agatttaaga gcgtattata cgaaaccaag tttagaattt aaaaatgaga
     5941 ttggtattat tttaaaaaaa tggacgacaa taagatttat gaatgttgtc ccagattatt
     6001 tcatatataa aattgcttta gttggtaaag atgataaaaa atatggtgaa ggagtacata
     6061 ggaatgtcga tgtatttgtc gttttagaag aaaataatta caatctggaa aaatattctg
     6121 tcggtggtat cacaaagagt aatagtaaaa aagttgatca caaagcagga gtaagaatta
     6181 ctaaggaaga taataaaggt acaatctctc atgatgtttc agaattcaag attactaaag
     6241 aacagatttc cttgaaagaa cttgatttta aattgagaaa acaacttatt gaaaaaaata
     6301 atctgtacgg taacgttggt tcaggtaaaa ttgttattaa aatgaaaaac ggtggaaagt
     6361 acacgtttga attgcacaaa aaattacaag aaaatcgcat ggcagatgtc atagatggca
     6421 ctaatattga taacattgaa gtgaatataa aataatcatg acattctcta aatagaagct
     6481 gtcatcggaa aaacaagaag ttaagtgaca acggcctaca tgttgcttag cttcttttgt
     6541 tatgttcgat gatttgagaa cccgaatttt cgatgggtcc aaatatgacg tggaagagac
     6601 ctgaatttat ctgtaaatcc ctatctatcg ggtgtgaagc acaacgggat cagttttatt
     6661 taacgaacat tatagattcc ttaatttact taataatgat tcaatgatta ttaaacatgg
     6721 tttaatgtga aaggtcaaat acgctaacta taataaagct gtatgattca atagacgtaa
     6781 gcgaacaaat ctaataatta cgaatggagc atacaactat gaaaatgaca gcaattgcga
     6841 aagcaagttt agcattaggt attttagcaa caggaacaat aacgtcattg catcaaactg
     6901 taaatgcgag tgaacataaa gcaaaatatg aaaatgtgac aaaagatatc tttgacttaa
     6961 gagattacta tagtggcgca agtaaggaac ttaaaaatgt tactggttat cgttatagca
     7021 aaggtggcaa gcattacctt atctttgata aaaatagaaa attcacaaga gtacagatat
     7081 ttggtaaaga tattgaaaga tttaaagcac gtaaaaatcc gggattagac atatttgttg
     7141 ttaaagaagc ggaaaaccgt aatggcacag tgttttcata tggtggtgtc actaagaaaa
     7201 atcaagacgc ttattatgat tatataaacg caccaagatt tcaaatcaag agagatgaag
     7261 gtgacggtat tgctacgtac ggtagagtac actacattta taaagaagag atttcactta
     7321 aagaactcga ctttaaattg agacagtatt taattcaaaa ttttgatctg tataaaaagt
     7381 ttcctaaaga tagtaagata aaagtgataa tgaaagatgg cggctattat acgtttgaac
     7441 ttaataaaaa attacaaaca aatcgcatga gtgacgtcat tgacggtaga aatattgaaa
     7501 aaatagaagc caacattaga taattcaatg aaatatggat aatagtaaaa tatggatagt
     7561 atagaggagt taggcaacat aagttgctta gcttcttttt tgtgttggag agatgaaaat
     7621 gaagcgtatc gatgaataat aaaaacacca ataaaacttg tggaaatagt tgatacttat
     7681 agtcgcgcgt tgtccttttc gtgacatgaa acaatgtgga aaacatagtt aaattgaggg
     7741 aaagtgtgaa tagttaaaaa agctgcgtta agtttaaaaa atagattaac gctgttagga
     7801 ttccattaat tagcttaaca ttggttcaaa aatagttaaa aagaggttaa ttcatagctt
     7861 agtattacgc ttatataatg atagtagatt gttcgtatta cgtaatt
//
LOCUS       DAXWFK010000116         7866 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.116, whole genome shotgun sequence.
ACCESSION   DAXWFK010000116 DAXWFK010000000
VERSION     DAXWFK010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7866
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.116"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(48..197)
                     /gene="rpmG"
                     /locus_tag="ACGEXS_001957"
     CDS             complement(48..197)
                     /gene="rpmG"
                     /locus_tag="ACGEXS_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFF8012954.1"
                     /translation="MRVNVTLACTECGDRNYITTKNKRNNPERVEMKKFCSRENKQTL
                     HRETK"
     gene            complement(310..2385)
                     /locus_tag="ACGEXS_001958"
     CDS             complement(310..2385)
                     /locus_tag="ACGEXS_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFF8012955.1"
                     /translation="MLKRLKEKSNDEIVQNTINKRINFIFGVIVFIFAVLVLRLGYLQ
                     IAQGSHYKQIIKNDENITVNESVPRGRILDRNGKVLVDNASKMAITYTRGRKTTQSEM
                     LDTAEKLSKLIKMDTKKITERDKKDFWIQLHPKKAKAMMTKEQAMLADGSIKQDQYDK
                     QLLSKIGKSQLDELSSKDLQVLAIFREMNAGTVLDPQMIKNEDVSEKEYAAVSQQLSK
                     LPGVNTSMDWDRKYLYGDTLRGIFGDVSTPAEGIPKELTEHYLSKGYSRNDRVGKSYL
                     EYQYEDVLRGKKKEMKYTTDKSGKVTSSEVLNPGARGQDLKLTIDIDLQKEVEALLDK
                     QIKKLRSQGAKDMDNAMMVVQNPKNGDILALAGKQINKSGKMTDYDIGTFTSQFAVGS
                     SVKGGTLLAGYQNKAIKVGETMVDEPLHFQGGLTKRSYFNKNGHVTINDKQALMHSSN
                     VYMFKTALKLAGDPYYSGMALPSDISSPAQKLRRGLNQVGLGVKTGIDLPNETRGQIE
                     PLTNNPGNYLDLSIGQYDTYTPLQLSQYVSTIANDGYRIQPHIGLTIHESTNKDEVGP
                     LKKKINGTVLNKVNNTEKEIKQIQEGFKMAFNDKDGTGYVSFKDTVVPTAGKTGTAEV
                     FQNGEPRVNSTYIGYAPIDDPKLAFSIVYTNQPVPPPWLTGGDLGRDVINYYFKQLGK
                     DDKNKDKDK"
     gene            complement(2506..3105)
                     /locus_tag="ACGEXS_001959"
     CDS             complement(2506..3105)
                     /locus_tag="ACGEXS_001959"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFF8012956.1"
                     /translation="MAFELPKLPYAFDALEPHFDKETMEIHHDRHHNTYVTKLNAAVE
                     GTDLESKSIEEIVANLDSVPANIQTAVRNNGGGHLNHSLFWELLSPNSEEKGTVVEKI
                     KEQWGSLEEFKKEFADKAAARFGSGWAWLVVNNGQLEIVTTPNQDNPLTEGKTPILGL
                     DVWEHAYYLKYQNKRPDYIGAFWNVVNWEKVDELYNATK"
     gene            complement(3381..3791)
                     /locus_tag="ACGEXS_001960"
     CDS             complement(3381..3791)
                     /locus_tag="ACGEXS_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFF8012957.1"
                     /translation="MNTNDAIKILKENGLKYTDKRKDMLDIFVEEDKYINAKYIQQVM
                     DENYPGISFDTIYRNLHLFKDLGIIENTELDGEMKFRIACTNHHHHHFICEKCGDTKV
                     IDYCPIDQIKLSLPGVNIHKHKLEVYGVCESCQD"
     gene            complement(3778..4641)
                     /locus_tag="ACGEXS_001961"
     CDS             complement(3778..4641)
                     /locus_tag="ACGEXS_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFF8012958.1"
                     /translation="MIDALLNFDFMRYSLISGILIGFIAPLIGAFIVVRRLSLIADAL
                     SHVTLGGISFGMFLLTIMPTLVFINPMWFGILFAIVGALLIEKLRTSYTAYQEIAIPI
                     IMSAGIALSAIFISLADGFNQEIVGLLFGSISAVNISDLTTIIVIAIIVVLFITLFYK
                     ELFILSFDEEYSKVIGIPKWIQFLFIVIVAMVISASMRVVGILLVSALITLPIAISMR
                     ITKSFKQLILLSVFLGELSVILGLVLAFYMDISPGGVIVVLLVILLMITMAYQKMRMK
                     FKKGANINEYK"
     gene            complement(4683..5468)
                     /locus_tag="ACGEXS_001962"
     CDS             complement(4683..5468)
                     /locus_tag="ACGEXS_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFF8012959.1"
                     /translation="MRGENTMTPVFELKNVNYYYDHKKVLENINIKINKGEFLAIVGP
                     NGAGKSTLLKLILGLLPLQSGEIFVEGIDFKNKKTSIKLSYVSQKANAFNSGFPASVK
                     EVVLSGLTKTKRLFQTFNSKDNEKVIKVLERLNISDLIHKNIAELSGGQQQRVMIARA
                     LISEPAVLVLDEPTNGIDAKHVSEFYNTLDQLKQEGITIILVTHDIGVVADTATEVAC
                     LNKHLHFHGTTDEFKSLDEVEISKIYGHPVRFVDHQHNRECCN"
     gene            complement(5594..6484)
                     /locus_tag="ACGEXS_001963"
     CDS             complement(5594..6484)
                     /locus_tag="ACGEXS_001963"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFF8012960.1"
                     /translation="MLLGSHVSMSGKKMLEGSAIEAHEYGETTFMIYTGAPQNTRRKS
                     IEDLNITKGHEVMEKYGLSNIVVHAPYIINIANTTKPETFNLGVDFLQQEIERTQAIG
                     AKDIVLHPGAHVGAGVDAGINKIIEGLNEVLTNDNNVRIALETMAGKGTEIGRSFEEL
                     ARIIDGVHNNERLSVCFDTCHTHDAGYNVKEDFDGVLNEFDKIIGVDRIKVVHVNDSK
                     NDRGAQKDRHENIGFGYIGFDALNYIVHHDSFKDIPKILETPYVGEDKKNKKPPYKLE
                     IEMLKQQQFDPELKNKVMQQ"
     gene            complement(6494..7840)
                     /locus_tag="ACGEXS_001964"
     CDS             complement(6494..7840)
                     /locus_tag="ACGEXS_001964"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFF8012961.1"
                     /translation="MAKHPFEQFNLESSLIDAVKDLNFEKPTEIQNRIIPRILKRTNL
                     IGQSQTGTGKSHAFLLPLMQLIDSEIKEPQAIVVAPTRELAQQLYDAANHLSQFKAGV
                     SVKVFIGGTDIEKDRQRCNAQPQLIIGTPTRINDLAKTGHLHVHLASYLVIDEADLMI
                     DLGLIEDVDYIAARLEDNANIAVFSATIPQQLQPFLNKYLSHPEYVAVDSKKQNKKNI
                     EFYLIPTKGAAKVEKTLNLIDILNPYLCIIFCNSRDNANDLARSLNEAGIKVGMIHGG
                     LTPRERKQQMKRIRNLEFQYVIASDLASRGIDIEGVSHVINFDVPNDIDFFTHRVGRT
                     GRGNYKGVAITLYSPDEEHNISLIEDRGFVFNTVDIKDGELKEVKAHNQRQARMRKDD
                     HLTNQVKNKVRSKIKNKVKPGYKKKFKQEVEKMKRQERKQFSKQQNRQKRKQNKKG"
BASE COUNT         2406 a         1455 c         1065 g         2940 t
ORIGIN      
        1 ttgagatttc acctttttta ttgttaattt aaatttaatt ttaatgatta ttttgtttca
       61 cggtgtaaag tttgtttgtt ttcacgtgaa cagaatttct tcatttcaac acgttctgga
      121 ttatttcttt tgttcttagt tgtaatgtag tttctgtcac cacattccgt acaagctaaa
      181 gttacgttta cgcgcatggt tattcctcct tatcatttct aaatacgact ttattattat
      241 accaacatct ataatatttg caaagttaaa attttacaac catgcgctac acaatcgtca
      301 ggttaaattt tatttgtctt tgtctttatt tttatcatct ttacctaact gcttaaagta
      361 gtagttaatt acatctctac ctaagtctcc acctgttaac catggtggtg gtacaggctg
      421 atttgtatat acaattgaaa acgctaattt tggatcatca attggcgcgt atcctatata
      481 agtagagtta actcttggct ctccgttttg gaacacttct gcggtacccg ttttaccagc
      541 agtaggtact actgtatctt taaaactaac atatccagta ccatctttat cattaaatgc
      601 cattttgaat ccttcttgaa tttgtttgat ttccttttca gtattattaa ccttgttcaa
      661 gacagtgcca ttaattttct tcttgagtgg accaacctca tctttattag ttgattcatg
      721 aatcgttaat ccaatgtgtg gctgtattct ataaccatca ttcgctatag ttgaaacata
      781 ttgtgataat tgtaatggtg tataggtatc atattgacca attgataaat ctagataatt
      841 acctggatta tttgttaatg gttcgatttg acctcttgtt tcatttggta aatctatccc
      901 tgttttcaca cctaagccta cttgatttaa tcctcttctt agcttttggg caggtgaact
      961 tatgtctgaa ggtaaagcca taccagaata ataagggtct cccgctaatt ttaatgctgt
     1021 tttaaacata tatacgtttg atgaatgcat caaagcttgc ttatcattaa tagttacatg
     1081 cccgttttta ttgaagtatg atcgttttgt caaaccacct tggaaatgta atggttcatc
     1141 gaccattgtt tctccaactt tgatagcttt attctgataa ccggctaata atgttccacc
     1201 ttttacagaa gatccaaccg caaattgaga agtaaacgta ccaatgtcat aatcagtcat
     1261 tttaccactc ttattaatct gctttccggc aagcgcaaga atgtctccat ttttaggatt
     1321 ttgtacaacc atcattgcat tatccatatc tttggcacct tgactgcgaa gcttcttaat
     1381 ttgtttatct aataatgctt ctacttcttt ttgaagatct atatcgatcg ttaatttcaa
     1441 atcttgaccg cgagcgccag gatttaacac ttctgaagat gtaactttac cagatttgtc
     1501 cgttgtgtat ttcatttctt tcttcttacc acgcaataca tcttcatatt gatattctag
     1561 gtaagatttt ccaacacgat cattgcgtga atatcctttg gataagtaat gttctgtcaa
     1621 ttcttttgga ataccttcag caggtgtcga tacatctccg aatatacctc ttaaagtatc
     1681 gccatataga tattttctat cccaatccat agacgtgtta acacctggta atttggaaag
     1741 ttgctgagaa actgctgcat actctttttc actgacatct tcatttttta tcatttgtgg
     1801 atctaaaact gttcctgcat tcatctctcg aaaaatagct aaaacttgta aatctttaga
     1861 agacaattca tctaattgtg attttccgat tttcgataac agttgtttat catattgatc
     1921 ttgtttaata cttccatctg ctaacatagc ttgttctttt gtcatcattg cttttgcttt
     1981 tttaggatgc aactgaatcc agaaatcttt cttatcacgt tctgtaattt tcttagtatc
     2041 catcttgatt agctttgata acttttcagc cgtatccaac atttccgatt gtgttgtttt
     2101 tcgaccccta gtatatgtaa tagccatttt agaagcatta tcaactaaaa ctttcccatt
     2161 tctgtctaaa atacgacctc ttggcacaga ctcattcact gtaatgtttt catcattttt
     2221 tataatttgt ttataatgtg agccttgtgc gatttgtaaa taacctaaac gtagtactag
     2281 tactgcaaaa ataaatacaa tcacaccaaa tataaagtta attctcttgt taattgtatt
     2341 ttgaacgatt tcatcatttg atttttcttt tagtcttttt aacaaaacta cctacctcta
     2401 ttcaaagtct ttcactttaa atcatatatg aatttagaaa ttatttctat ctttttgaca
     2461 aaaaaataac ggtctcattt aagagaccga acaagtaatc atactttatt ttgttgcatt
     2521 atataattcg tcaacttttt cccagttaac tacattccaa aatgcgccaa tgtagtcagg
     2581 gcgtttgttt tgatatttta ggtaataagc gtgttcccat acgtctaaac ctaaaatagg
     2641 tgttttaccc tcagttaatg gattatcttg gtttggtgta gtcacaattt ctaactggcc
     2701 attgtttacg actaaccaag cccaacctga accaaagcgt gcagctgctt tgtcagcaaa
     2761 ttctttttta aattcttcta aagaacccca ttgttcttta attttttcta ctacagtacc
     2821 tttttcttct gagtttggtg aaagtaactc ccagaataat gaatggttta aatgtccacc
     2881 gccattatta cgtacagcag tttggatgtt agctggtaca ctgtctaaat tagcaacaat
     2941 ttcttcaata gatttagatt ctaaatctgt accttctact gcagcattta atttcgtaac
     3001 atacgtgtta tgatgtctgt catggtgaat ttccatagtt tctttgtcaa aatgtggttc
     3061 taatgcatca aatgcgtatg gtaattttgg taattcaaaa gccataaata atcatcctcc
     3121 taaaatgtct gtaagtaaat aataacaagc agtgactggt tcaacaaaga atttgcttaa
     3181 attctactac ttattatttt ctctactcat ttaatataac tcaaatcaaa aataattaaa
     3241 cattttgtat ataaaaagtt aacagatttg ccataaaatc atacgaacgg agtatgaaat
     3301 gaacctttat cttctataat ttaaaaaatg agatttatgc atacatctga ccaaatgtgc
     3361 ataaatctca tttctttata ttaatcttgg caagactcac atacaccata aacttcaagt
     3421 ttgtgtttgt gaatattaac accaggtagt gataatttta tctgatctat tggacaataa
     3481 tctattacct ttgtatctcc acacttttca cagataaaat gatgatgatg atggtttgta
     3541 caagcgattc taaacttcat ttcaccatca agttctgtat tttcaataat tcctaaatct
     3601 ttaaataagt gcaggtttct atatattgtg tcgaatgaaa ttccaggata attttcatcc
     3661 ataacttgtt gtatatactt tgcgtttata tacttatctt cttcgacaaa aatatctaac
     3721 atatctttac gtttatctgt atattttaaa ccgttctctt ttaaaatttt aatagcatca
     3781 tttgtattca ttgatattag ctcccttttt aaacttcatt cgcattttct gataagccat
     3841 tgtaatcata agtaaaataa caagtagaac tacaattaca ccacccggag aaatgtccat
     3901 atagaaagct aggactaagc ctaatattac tgataattca cctaaaaata cacttagtaa
     3961 tatcaattgc ttaaaacttt ttgttattcg catacttatt gcaattggta acgtgattaa
     4021 cgcacttact aacagtatcc ctacaacacg cattgaggca gaaataacca tcgctacaat
     4081 aacaataaat aaaaattgaa tccatttagg aatgccaatg actttactat attcctcatc
     4141 aaatgacaat ataaataatt ctttataaaa caatgtaata aacagaacaa ctatgatggc
     4201 aatgacaata atcgttgtta aatcacttat attcactgcg cttattgagc caaatagcaa
     4261 tccaacaatt tcttgattga acccatcagc taatgaaatg aagattgcac tcaaggcgat
     4321 accagcactc attataattg gaatagcaat ttcttggtaa gcagtgtatg acgttcttaa
     4381 tttttcaatt agaagcgcac ctactattgc gaataagatt ccaaaccaca ttggattaat
     4441 aaatactagt gttggcataa tagtaagtaa aaacataccg aaagatatac cacctaaagt
     4501 tacatgactt agagcatcag ctataagtga tagtcgtcta acaacgataa aagcaccgat
     4561 tagaggcgca ataaaaccta tcaagatacc actaattaaa gagtacctca taaaatcaaa
     4621 attcaataat gcatctatca attgtgacac gcctttccat tttaaataaa ctcaaatctt
     4681 tattaattac aacattctcg attatgctga tgatcgacaa aacgtacagg atgtccataa
     4741 atttttgaaa tttcaacttc atcaagtgat ttaaactcat cagttgtacc atggaaatgc
     4801 aaatgcttat ttaaacatgc tacttcagta gcagtatctg ctacaacacc gatatcatga
     4861 gtaactaaga taatggtgat accttcttgt tttaattgat ctaaagtatt ataaaattca
     4921 cttacatgtt ttgcatcaat accattcgtt ggttcatcaa gtactaatac tgcaggttct
     4981 gaaatcaatg ctcgagcaat cattacacgt tgttgttgac cacctgataa ttctgctata
     5041 tttttatgaa ttaaatcact tatattcagt ctttctagta ctttaatcac tttttcatta
     5101 tctttgctat taaatgtttg gaaaagacgt tttgtctttg ttaatccgct taaaacaact
     5161 tctttaacac ttgctgggaa acctgaatta aaggcatttg ctttttgtga tacatagctt
     5221 aatttaattg atgttttctt atttttaaaa tcaatacctt caacaaaaat ctcaccactt
     5281 tgtaaaggta ataaccctag aatcaacttc aataatgttg atttaccagc accatttggt
     5341 ccaacaattg ctaaaaattc acctttattt attttaatgt ttatattttc taacactttt
     5401 ttatgatcat agtagtaatt gacatttttc aattcaaaga ctggtgtcat cgtattctca
     5461 cctcgcattc aactatacaa ctcctagtaa catatgtaaa cagtaatgtt tacgactcaa
     5521 aattagacaa aataaagaga tatgccccct tcaagtttta tttatcgcat ttcttgaaga
     5581 gagcattatc attttattgt tgcataacct tattttttaa ttctgggtca aattgctgtt
     5641 gttttaacat ttcaatttca agtttatatg gcggtttttt atttttctta tcttcaccaa
     5701 cataaggtgt ttctaagatt ttcggaatat ctttaaaact atcatgatgc acaatgtaat
     5761 ttaatgcatc aaaaccaatg taaccgaagc caatattttc atgtcggtct ttttgagcgc
     5821 cacggtcatt tttagaatca ttgacatgaa caactttgat tctgtcgact ccaatgattt
     5881 tatcaaattc atttaatacg ccatcaaagt cctctttaac attatatcca gcatcatgcg
     5941 tatgacatgt atcaaaacat actgataaac gttcgttatt atgaactcca tcaataatac
     6001 gtgctaactc ttcaaatgag cgaccaatct ctgtaccttt acctgccatc gtttcaagcg
     6061 caatacgtac attattgtca ttcgttaaaa cttcatttaa tccttcaata atcttattaa
     6121 ttccggcatc aacaccagct ccaacatgcg cacctggatg taatacaata tctttagccc
     6181 ctatagcttg cgttctttca atttcttgtt gcaagaaatc tacaccaaga ttaaacgttt
     6241 ctggtttggt tgtatttgca atattaatga tgtatggtgc atgaacaaca atattagata
     6301 aaccatattt ttccatcact tcatgacctt tagttatatt taaatcttca atacttttac
     6361 ggcgcgtgtt ttgaggtgca ccagtataaa tcataaatgt tgtttcacca tattcatgcg
     6421 cttctatagc agaaccttct aacatctttt taccactcat tgaaacatgt gatcctaata
     6481 acataaaaca cacctaacct tttttgtttt gcttacgttt ttgtctattt tgctgcttac
     6541 taaattgctt acgctcttga cgtttcattt tttcaacttc ttgtttaaat ttcttcttat
     6601 aacctggttt aactttgttt ttaattttac ttcgaacttt gttcttcact tgattagtta
     6661 aatggtcatc tttgcgcatt cttgcttgac gctgattgtg cgctttaact tcttttaact
     6721 caccatcttt aatatcaaca gtattgaata caaaaccgcg atcttctatt aatgaaatat
     6781 tgtgttcttc atcaggacta taaagcgtaa ttgctacacc tttataattc ccacgaccag
     6841 ttcgtccgac tctatgcgta aagaagtcaa tatcatttgg cacatcaaaa ttgatgacat
     6901 gactaacacc ttcaatatca ataccacgag atgctaaatc gctggcaata acgtattgga
     6961 attctaaatt acgtatacgt ttcatttgtt gtttacgttc acgtggcgtt aagccaccat
     7021 gaatcatacc aactttaata ccagcttcat ttagtgaacg tgctaaatca tttgcattat
     7081 ctctactatt acagaaaata atacataagt atggatttag tatatcaatt aaatttaaag
     7141 tcttttcaac tttagctgca cctttagtag gtattaaata gaattcgatg ttctttttat
     7201 tttgtttttt actgtcgaca gctacatatt ctggatgact taaatattta tttaaaaatg
     7261 gttgtaactg ttgtgggatt gtagcactaa acaccgcaat atttgcatta tcttccaatc
     7321 ttgcagcaat gtaatctaca tcttcaatta atcctaagtc aatcataaga tccgcttcat
     7381 caataactaa atatgatgct aagtgcacat gtaaatgtcc cgttttagct aagtcattaa
     7441 ttctagtagg ggtgcctata atcaattgtg gttgtgcatt acaacgttgt ctatctttct
     7501 ctatatctgt accaccaata aaaactttaa ctgaaacacc agctttaaat tggcttaaat
     7561 ggttcgctgc atcgtatagt tgttgtgcaa gttctcttgt tggtgcaact acgattgctt
     7621 gtggttcttt tatttcacta tcaattaact gcattaatgg taataaaaat gcatgagatt
     7681 tccctgtacc cgtttgagat tgaccaatta aatttgttct ctttagtatt cttggaataa
     7741 ttcgattctg aatttcagtt ggtttttcaa aattaaggtc tttcacagcg tcaattaaac
     7801 tagattctag attaaattgt tcgaatggat gttttgccat gatttggcct ccttatatgt
     7861 tcatct
//
LOCUS       DAXWFK010000117         7817 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.117, whole genome shotgun sequence.
ACCESSION   DAXWFK010000117 DAXWFK010000000
VERSION     DAXWFK010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7817
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.117"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            130..1272
                     /gene="bshA"
                     /locus_tag="ACGEXS_001965"
     CDS             130..1272
                     /gene="bshA"
                     /locus_tag="ACGEXS_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HFF8012962.1"
                     /translation="MKIGITCYPSMGGSGIIATELGIKLAERGHEVHFITSNIPFRIR
                     KPLPNMIFHQVEVNQYAVFQYPPYDITLSTKIAEVIKEYDLDLLHMHYAVPHAICGIL
                     AREMSGKDIKIMTTLHGTDITVLGYDHSLQGAIKFGIEKSDIVTSVSKSLAQETHEII
                     ETNKEIIPIYNFVRENEFPTKHNTALKSQFGIAPDEKVLIHVSNFRQVKRIDTIIETF
                     AKVREKIPSKLILLGDGPELVPMRQLTKELNVEEDVLFLGKQDCVSEFYQLSDLVLLL
                     SEKESFGLTLLEAMKTGVVPIGSNAGGIKEVIKHGETGFVVDVGDCDSASDYAIRLLE
                     DKVLYNKLQKNMLADIAERFGSELITDQYEYYYQKMLNEHNKSKGE"
     gene            1277..2479
                     /locus_tag="ACGEXS_001966"
     CDS             1277..2479
                     /locus_tag="ACGEXS_001966"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFF8012963.1"
                     /translation="MDKSLFEQARPILEQIQDNGFEAYYVGGSVRDYVMGRNIHDIDI
                     TTSATPDEIESIFSHTIPVGKEHGTINVVFNDENYEVTTFRAEEDYVDHRRPSGVTFV
                     RDLYEDLQRRDFTMNAIAMDTAYKLYDYFDGQQDINNRIIRTVGIAEERFQEDALRMI
                     RCLRFQSQLSFDIATETFEAMRIQMADIKFLSIERIVIELTKLMRGINVEKSFNHLKS
                     LKAFNYMPYFEHLDMNQINVTEAIDLELLIAIVSVKFDINYSLKPLKLSNRQVKDINQ
                     YIQIMNALPSIITKEQLKMFVYDYDTHLIKNVMVAADVLKANDIQGHEPLIVNLQTID
                     ETLHRLPMHNRKDMMVNGGVLMAHLNAKSGPWLKDVLRQIEIAIVTGKVSNEETEILK
                     WVDNHVKI"
     gene            2466..3437
                     /locus_tag="ACGEXS_001967"
     CDS             2466..3437
                     /locus_tag="ACGEXS_001967"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFF8012964.1"
                     /translation="MSKYSQDVLQLLYKNKPNYISGQSIAESLNISRTAVKKVIDQLK
                     LEGCKIDSVNHKGHLLQQLPDIWYQGIIDQYTKSSALFDFSEVYDSIDSTQLAAKKSL
                     VGNQSSFFILSDEQTKGRGRFNRHWSSSKGQGIWMSVVLRPNVAFSMISKFNLFIALG
                     IRDAIQHFSQDEVKVKWPNDIYIDNGKVCGFLTEMVANNDGIEAIICGIGINLTQQLE
                     NFDESIRHRATSIQLHDKNKLDRYQFLEILLQEIEKRYNQFLTLPFSEIREEYTAASN
                     IWNRTLLFTENDKQFKGQAIDLDYDGYLIVRDEAGESHRLISADIDF"
     gene            3461..6154
                     /locus_tag="ACGEXS_001968"
     CDS             3461..6154
                     /locus_tag="ACGEXS_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFF8012965.1"
                     /translation="MGMATYAVVDLETTGNQLDFDDIIQIGITFVRNNQIIDTYHSMI
                     RTNLEIPPFIQALTSIEENMLQQAPYFNQVAQEIYDKIKDCIFVAHNVDFDLNFIKKA
                     FKDCNIQYRPKKVIDTLEIFKIAFPTDKSYQLSELAEAHGITLANAHRADEDAATTAK
                     LMILAFEKFEKLPLDTLKQLYYLSKQLKYDLYDIFFEMVRQYDAKPLDKSYEKFEQII
                     YRKQVDFKKPTTNYNGSLKSLYSKAVDQLGLTYRPQQLYLAETILDQLMHSEKAMIEA
                     SLGSGKSLAYLLAALMYNIETGKHVMISTNTKLLQSQLLEKDIPAMNEALNFKINALL
                     IKSKSDYISLGLISQILKDDTSNYEVNILKMQLLIWITETPSGDIQELNLKGGQKMYF
                     DQKIETYVPARHDVHYYNFIKRNAQNIQIGITNHAHLIHSDVENSIYQLFDDCIVDEA
                     HRLPDYALNQVTNELSYADIKYQLGLIGKNENEKLLKAIDQLEKQRILEKLDIAPIDI
                     FGLKASMNEIHELNEQLFSTIFTIINDSDVYDDDIHRFHNVFTFETKDILKDLHAIID
                     KLNKTLEIFNGISHKTVKSLRKQLLYLKDKFKNIEQSLKAGHTSFISIKNLSQKSTIR
                     LYVKDYAVKDVLTKQVLEKFKSLIFISGTLKFNHSFEAFKQLFNKDVHFNTFEVNTSL
                     QSAKNTSVFIPSDVASYQYKNIDEYVASIVSYIIEYTTITSSKCLVLFTSYKMMHMVQ
                     DMLNELPEFEDYVVLTQQQNQNYKIVQQFNNFDKAILLGTSTFFEGFDFQANGIKCVM
                     IAKLPFMNKHNAKYWLMDSEFTSTFKEYVLPDAVTRFRQGLGRLIRNENDRGIIVSFD
                     DRLINSNYKNFFEQTLENYRQKKGDIQQFGKLLRQIQKKKK"
     gene            6476..7768
                     /gene="asnS"
                     /locus_tag="ACGEXS_001969"
     CDS             6476..7768
                     /gene="asnS"
                     /locus_tag="ACGEXS_001969"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFF8012966.1"
                     /translation="MKTTIKQAKDHLNQDVTIGAWLTNKRSSGKIAFLQLRDGTGFMQ
                     GVVVKSEVDEEVFKLAKEITQESSLYVTGTITEDNRSDLGYEMQVKSIEVISEAHDYP
                     ITPKNHGTEFLMDHRHLWLRSKKQHAVMKIRNEVIRATYEFFNKDGFTKVDPPILTAS
                     APEGTSELFHTKYFDQDAFLSQSGQLYLEAAAMAHGKVFSFGPTFRAEKSKTRRHLIE
                     FWMIEGEMAFTNHAESLEIQEQYVTHVVKSVLENCKLELKILERDTSKLEKVATPFPR
                     ISYDDAIEFLKAEGFDDIEWGEDFGAPHETAIANHYDLPVFITNYPTKIKPFYMQPNP
                     ENEETVLCADLIAPEGYGEIIGGSERVDDLELLEQRVKEHGLDEEAYSYYLDLRRYGS
                     VPHCGFGLGLERTVAWISGVEHVRETAPFPRLLNRLYP"
BASE COUNT         2913 a         1040 c         1419 g         2445 t
ORIGIN      
        1 ttatatatac aacacctgtt tttagatata tgaaattata aactcaacat tactataaac
       61 ttaaatttaa acaattatct acgcatcgag aataatagag agaaatagaa agaaaggatg
      121 ggcaagcgaa tgaagatagg tataacatgt tacccgtcca tgggtggttc tggaattatt
      181 gccacagaat taggaattaa attagcagag cgaggtcacg aagtacattt tatcacttca
      241 aatatcccgt ttagaataag aaaaccatta ccgaatatga tttttcatca agttgaagtg
      301 aatcaatatg cagtatttca gtatccaccg tatgatatta cgttaagtac taaaatcgct
      361 gaagttatta aagaatatga tcttgatttg ttgcatatgc actatgctgt acctcatgca
      421 atttgtggga ttttagctcg tgagatgtca ggtaaagata taaaaattat gacaacgcta
      481 cacggtactg atattactgt tttgggctat gatcattcac tccaaggtgc aattaaattt
      541 ggcattgaaa aaagtgatat tgtgacaagt gtgagtaaat ctttagcaca agaaacacat
      601 gaaattattg aaactaacaa agaaattata ccgatttata attttgtacg tgaaaatgag
      661 tttccaacta aacataacac agcattaaaa tcacaatttg gtatagcacc agatgaaaaa
      721 gtgttaatac atgtatcgaa ttttagacaa gtaaaacgca tagatactat tatagaaaca
      781 tttgcaaaag tacgtgaaaa gatacctagt aaattaattt tacttggaga tggtcctgaa
      841 ttagtgccaa tgcgtcaatt gacgaaagag ttaaacgtag aagaggatgt tttattttta
      901 gggaaacaag attgcgtaag tgaattttac caattatctg acttagtatt attattaagt
      961 gaaaaagaaa gctttggact aactttactt gaggctatga aaacaggcgt cgttccaatt
     1021 ggttcaaatg ccggtggaat taaagaggtt attaaacatg gtgaaactgg atttgtcgta
     1081 gatgtgggtg attgtgattc agcgagtgac tatgctatcc gattacttga agataaggtg
     1141 ttatacaaca aacttcagaa aaacatgcta gcagatattg ctgaacgttt tggatcagaa
     1201 cttattacag atcaatatga gtattattat caaaaaatgc taaatgagca taacaaaagt
     1261 aaaggcgagt aataatatgg ataaatcatt atttgaacag gcaaggccta tattagaaca
     1321 aattcaagac aatggttttg aagcatatta tgtaggtggc tctgtaagag attatgtcat
     1381 gggaagaaat attcatgata tagatatcac aacaagtgca acgccggatg aaatagaatc
     1441 tatctttagt catacgatac ctgtaggtaa agaacatggc acgataaatg tagtttttaa
     1501 tgatgaaaat tatgaagtga caacattccg ggctgaagaa gattatgtcg atcaccgtag
     1561 accaagtggt gttacatttg ttcgtgattt atacgaagat ttgcaacgac gagatttcac
     1621 gatgaatgcg atagcaatgg atacagcata caaattgtat gattattttg atggtcaaca
     1681 agatattaat aatcgaataa taagaactgt aggtatagct gaggaacgat tccaagaaga
     1741 tgctttacgt atgattcgat gtttaaggtt ccagtcacaa ttatcatttg atattgcaac
     1801 ggaaacattc gaagcgatgc gtatacaaat ggcagatatt aaatttttat caattgagcg
     1861 tatagtgatt gaactaacta aattaatgcg aggtattaat gttgaaaaga gttttaatca
     1921 tttaaaatcg ctgaaagcat ttaattatat gccgtatttc gaacatcttg atatgaatca
     1981 aattaatgta actgaagcaa ttgatttaga attgttgatt gctatagtat cagttaaatt
     2041 tgatattaat tactcattga agcctttaaa gctaagtaac cgacaagtaa aagatatcaa
     2101 tcaatatatt caaattatga atgcattacc aagtattatt acaaaagaac aattaaaaat
     2161 gtttgtttat gattatgata cgcatctcat taaaaatgta atggttgcag cagacgtgtt
     2221 aaaggcaaat gatattcaag gacatgaacc attaatcgtt aaccttcaaa cgattgatga
     2281 aacattacat cgtttaccta tgcataatag aaaagacatg atggttaatg gcggtgtact
     2341 tatggcacat ttaaatgcca aaagtggtcc gtggttaaaa gatgtgctaa gacaaattga
     2401 gatagcgatt gtaacaggta aagtaagcaa cgaagaaact gaaattttga aatgggtgga
     2461 taatcatgtc aaaatatagt caagatgtac ttcaattact ctataaaaat aaaccgaatt
     2521 atatatctgg acaaagcatt gcggaatcac ttaatatttc acgcactgca gtaaaaaaag
     2581 tgattgacca attaaagtta gagggatgta aaatagattc agtaaatcat aaagggcatt
     2641 tattacaaca gctcccagat atttggtatc aaggtataat agaccaatat acaaaaagtt
     2701 ctgctttgtt tgattttagt gaagtatacg attcaataga ttctacacaa cttgctgcga
     2761 aaaagtcact tgttggaaat caatcttcat tttttatctt gagtgatgaa caaacgaaag
     2821 gtcgtgggcg atttaataga cattggagtt cttcaaaagg gcaaggaatt tggatgtctg
     2881 tcgtgttaag acctaacgtt gcattctcaa tgatatctaa atttaattta tttattgcat
     2941 tagggataag agatgcgatt caacatttta gtcaagatga agtcaaagtg aaatggccga
     3001 atgatatata tattgataat ggtaaagtgt gtggtttctt aactgaaatg gttgctaata
     3061 atgatggtat agaagcaata atatgtggta taggtattaa tttgacgcaa caactagaaa
     3121 actttgatga aagtattaga catagagcaa caagtataca attacatgat aaaaataaat
     3181 tagatagata tcaattttta gagatattac ttcaagaaat tgaaaaaaga tataatcaat
     3241 ttttaacgtt acctttttct gaaattcgtg aagaatatac tgcagcttct aatatttgga
     3301 atagaacgtt gctatttaca gaaaatgata aacagtttaa aggacaagca attgatttag
     3361 attacgatgg ctatctaatt gttagagatg aagcgggtga atcacaccgt ttaattagtg
     3421 cagatataga tttttaacac taaagcaagg agagatagct atgggtatgg caacctatgc
     3481 cgttgtggat ttggaaacaa caggcaacca attagatttt gacgatatca ttcaaattgg
     3541 tataacgttt gtgcgcaata accaaattat tgatacatat cattctatga ttagaacaaa
     3601 tttagagatt ccgccgttca ttcaagcgtt aacatccatt gaagaaaata tgctacaaca
     3661 agcgccatat tttaatcaag ttgcacaaga aatctacgat aagataaaag attgcatttt
     3721 tgtagctcat aatgtagatt ttgatttgaa ttttattaaa aaggcattta aagattgtaa
     3781 tattcaatat cgtcccaaaa aagttataga tacactagaa atctttaaaa tagcttttcc
     3841 tacagataaa agctatcaat taagtgaatt agcagaggca catggtatta ccttagcaaa
     3901 tgcccatcga gctgacgaag atgccgctac tactgctaaa ttgatgatat tagcttttga
     3961 aaaatttgaa aagttaccgt tagatacttt gaagcaatta tattatttaa gtaaacagtt
     4021 gaaatacgat ttgtatgata ttttctttga gatggttagg caatatgatg caaagccatt
     4081 ggataaatcg tacgaaaagt tcgaacagat tatctatcgt aagcaagttg attttaaaaa
     4141 gccgacaaca aattataatg gcagcttgaa gtctttatat agcaaagctg tagatcaact
     4201 tggcttaaca tatcgaccgc aacaattata tttagcggaa actatattag atcagctcat
     4261 gcatagtgaa aaagcaatga ttgaagcatc actaggcagt ggtaaatcat tagcatattt
     4321 acttgcggcc ttgatgtata atattgaaac tgggaagcat gtcatgattt ctaccaatac
     4381 taaattactg caaagtcaat tgttagaaaa agatattcca gcaatgaatg aagcgttgaa
     4441 ttttaaaatt aatgcactat tgattaaaag taaaagtgat tacatttcat tggggcttat
     4501 tagtcaaatt ttaaaagatg acacaagtaa ttacgaagtg aatatcttga aaatgcaatt
     4561 gctgatttgg attacagaga cgccgtccgg agatatacag gaattaaatt taaaaggtgg
     4621 tcaaaagatg tattttgacc agaaaattga aacatatgta ccggcacgtc acgatgttca
     4681 ttattataat tttattaagc gaaatgcaca gaacattcaa attggtatta caaatcatgc
     4741 acatttaatt cattctgatg ttgaaaattc aatatatcaa ttgtttgatg attgtatagt
     4801 ggatgaagca catcgcttac cagactatgc cttaaatcaa gtgacaaatg agttgagtta
     4861 tgctgatatt aaatatcagc ttggtttgat tggtaaaaac gaaaatgaaa aattgttaaa
     4921 ggcaattgat caactggaaa agcagcgaat tttggaaaaa ttagatattg caccaattga
     4981 tatttttggc ttgaaggcta gtatgaatga gattcatgag ttaaatgagc aattgttctc
     5041 aacgatattt acgattatca atgattctga tgtttatgat gatgatattc atcgcttcca
     5101 taatgtattt acttttgaaa caaaagacat tttgaaggat ttacatgcga ttatcgataa
     5161 gttaaataaa acgctagaaa tattcaatgg cattagtcat aaaacagtta aatcattacg
     5221 gaaacagttg ttgtatttaa aagataaatt taaaaatatc gaacaaagtt taaaagctgg
     5281 acatactagt ttcatttcga ttaaaaattt atctcaaaaa tcaacaatac gtttatatgt
     5341 gaaagattac gctgtaaaag atgtattaac gaaacaagta ttggaaaaat ttaaatcact
     5401 catttttata tctggtactt taaaatttaa tcattcgttt gaagctttta aacagttatt
     5461 caacaaagat gttcacttta atacatttga agttaacacg tcgttacaaa gtgcaaaaaa
     5521 tacaagtgta tttataccga gtgatgtagc atcttatcaa tataaaaata tagatgagta
     5581 tgtagcatcg attgtaagct atattattga atatacgact ataacgtcat caaaatgttt
     5641 agtcttattt acgagttata aaatgatgca tatggtacaa gatatgctaa atgaattgcc
     5701 agaatttgaa gattatgttg tattaacaca acagcagaac caaaattata aaatagtaca
     5761 acagtttaat aattttgata aggccatact gcttggtacg tcaacatttt ttgaaggttt
     5821 tgattttcaa gcaaatggta ttaaatgtgt gatgatagcg aagttaccgt ttatgaacaa
     5881 gcataatgca aaatattggc ttatggattc tgaattcact tcaactttca aggaatatgt
     5941 attacctgat gcagttacac gttttagaca aggacttgga agattaatta gaaatgaaaa
     6001 tgatcgcggc ataatagttt catttgatga tcgactcata aacagtaatt ataaaaactt
     6061 ttttgaacaa acacttgaaa attatcgtca gaaaaaaggc gacattcaac agtttggaaa
     6121 attattaaga caaattcaaa aaaagaaaaa gtgaaataca aagtaaaatt cttgtgacat
     6181 gacatgtgct atgttctgtt acaagaattt ttgtttggtc gtcaaaattt gagttttata
     6241 attaaacttg aatgttagca caaaaaacga ccttcatact aataatattg aggctttatt
     6301 atatattttg tctgaaactg atgattgtat tgtcttaata taaattaatg ttcaaatctg
     6361 tgcgtaaatc attaccattt acacgttatc ttttagtcta gttctagaac ttgaaagcgg
     6421 attttgttaa aatgaatgag tattgtataa cgaaaaaata aataggagaa tggttatgaa
     6481 aacaacgatt aaacaagcga aagatcattt aaaccaagac gttacaattg gtgcttggtt
     6541 aacaaataaa cgttcaagtg gtaaaatcgc ctttttacaa ttacgtgatg gaacaggctt
     6601 tatgcaaggc gtagtagtta aatcagaagt tgatgaagag gtattcaaac ttgcgaaaga
     6661 aattactcaa gaatcatctc tatacgttac aggcacaatt acagaagata atcgttctga
     6721 cttaggatac gaaatgcaag tgaaatcaat tgaagttatt tcagaagcac atgactatcc
     6781 gattacacct aaaaatcatg gtacagaatt cttaatggat caccgtcatt tatggttacg
     6841 ttctaaaaaa caacatgctg taatgaaaat tagaaatgaa gttattcgtg caacgtatga
     6901 atttttcaac aaagatggat ttacaaaggt tgatccacca attttgacag caagtgcgcc
     6961 agaaggtaca agtgaattat tccatactaa atactttgat caagatgcgt ttttatctca
     7021 aagtggtcag ttatacttag aagctgcagc aatggcacac ggaaaagtat tttcatttgg
     7081 tccaactttc agagctgaaa aatcaaaaac acgtagacac ttgatcgagt tctggatgat
     7141 tgaaggagaa atggctttca caaatcatgc tgaaagttta gaaattcaag aacaatatgt
     7201 aacacatgtt gtaaaatcag ttttagaaaa ttgtaaacta gagttgaaaa ttttagagcg
     7261 tgatacatca aaacttgaaa aagttgcgac accattccct agaatttcat atgatgatgc
     7321 aattgaattc ttaaaagcag aaggctttga tgatattgaa tggggcgaag attttggtgc
     7381 gccacatgaa acagccattg ctaatcatta tgatttaccg gtgtttatta ctaattatcc
     7441 aactaaaatt aagcctttct atatgcaacc aaatcctgaa aatgaagaaa ctgtattatg
     7501 tgcagactta attgcacctg aaggatacgg tgaaattatt ggtggatctg aacgtgtgga
     7561 tgacttagaa ttgttagaac aacgcgttaa agaacatgga ttagacgaag aagcatatag
     7621 ttactactta gacttacgtc gttatggtag tgtgccacac tgtggatttg gtttaggttt
     7681 agagcgtaca gtagcatgga tttctggtgt tgaacacgtt cgtgaaacag cgccattccc
     7741 aagattatta aaccgtttat atccataagt tttagggtcg ttacttaaaa tatatattat
     7801 gataataaaa agaatcg
//
LOCUS       DAXWFK010000118         7800 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.118, whole genome shotgun sequence.
ACCESSION   DAXWFK010000118 DAXWFK010000000
VERSION     DAXWFK010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7800
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.118"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            213..1670
                     /locus_tag="ACGEXS_001970"
     CDS             213..1670
                     /locus_tag="ACGEXS_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500488.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFF8012967.1"
                     /translation="MKCLIRFILVLGLLISSAMVYINPTAHAEQDQTWEKIKERGELR
                     VGLSADYAPMEFEHTVNGKTEYAGVDIDLAKKIAKDNNLKLKIVNMSFDSLLGALKTG
                     KIDIIISGMTSTPERKKQVDFSDSYMMTKNIMLVKKDKVNEYKDIKDFNNKKVGAQKG
                     TEQEKIAQTEIENASITSLSRLPDVILALKSGKVEGAVVEKPVAEAYLKQNPKLGISN
                     VKFNEEEKDTVIAVPKDSPKLLSQINKTIKEVKDKGLIDKYMTNAANAMNDDSGFISK
                     YGSFFLKGIKITILISLIGVALGSILGAFVALMKLSKIKIISWIASIYIEILRGTPML
                     VQVFIVFFGITAALGLDISALVCGTIALVINSSAYIAEIIRAGINAVDKGQMEAARSL
                     GLNYRQTMKSVIMPQAIKNILPALGNEFVTLIKESSIVSTIGVGEIMFNAQVVQGISF
                     DPFTPLIVAAALYFVLTFVLTRIMNMIEGRLNASD"
     gene            1663..2385
                     /locus_tag="ACGEXS_001971"
     CDS             1663..2385
                     /locus_tag="ACGEXS_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFF8012968.1"
                     /translation="MIKINNLNKVFGDNEVLKDINLEINQGEVVAIIGPSGSGKSTLL
                     RCMNLLEVPTKGQVIFEGNDLTEKGTQVDKLRQKMGMVFQNFNLFPHKKVVDNIILAP
                     KLLKKDNNDELHKEALSLLDKVGLKEKADVYPNQLSGGQKQRVAIARALAMHPDVILF
                     DEPTSALDPEVVGDVLKVMKDLAKEGMTMVVVTHEMGFAKDVSDKVIFMADGVVVESG
                     TPVEIFEQPQHERTQNFLARVL"
     gene            2536..3663
                     /gene="queG"
                     /locus_tag="ACGEXS_001972"
     CDS             2536..3663
                     /gene="queG"
                     /locus_tag="ACGEXS_001972"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFF8012969.1"
                     /translation="MDTKQLKQDIIDYAYTIGIDSIGFTTADPFDELKQKLEAYHANG
                     YASGFEESDIALRTEPKLSLPTARSIIAIAVGYPNKLKGAPKSVRGDRRGLFARASWG
                     QDYHTIMRKRLDMLAAFIESKVPDVEIKSMVDTGVLSDRAVAERAGLGFVGRNGFVIN
                     PKLGTWTYLGEMLVSIPFEPDDPLLDSCGDCTICVDRCPTSALVGNGQLNSQKCISFL
                     TQTKGYMPDQYRYKIGNRLYGCDTCQQVCPKNRGINTEQDDIILEPEILKPRLVPLLR
                     MSNKEFKQTYGHLAGAWRGKKPIQRNAILALAHFNEVDAIPELKKVATTDERPLIRAT
                     AYWAIGQILGEEARDFINANYDQEDAEVQNEMIKGLDTRRE"
     gene            3668..4138
                     /gene="trmL"
                     /locus_tag="ACGEXS_001973"
     CDS             3668..4138
                     /gene="trmL"
                     /locus_tag="ACGEXS_001973"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFF8012970.1"
                     /translation="MTNHIVLYQPEIPANTGNIARTCAGTNTHLHLIKPLGFRTDDKM
                     LKRAGLDYWEFVNITYHDSIEAFFEVTNGEYYLLTKFGKKTYSDFDFSNHDKDFYFIF
                     GKETTGLPDWVKEKYQDTALRIPMSEHIRSLNLSNTAALLIYEALRQQDFPGLN"
     gene            4297..4896
                     /locus_tag="ACGEXS_001974"
     CDS             4297..4896
                     /locus_tag="ACGEXS_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFF8012971.1"
                     /translation="MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAP
                     VLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGVPAGQVYPIAYEKDAIELIADKI
                     KTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
                     TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA"
     gene            4921..5073
                     /locus_tag="ACGEXS_001975"
     CDS             4921..5073
                     /locus_tag="ACGEXS_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAS053 family protein"
                     /protein_id="HFF8012972.1"
                     /translation="MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKN
                     SEEDSQ"
     gene            5850..6245
                     /locus_tag="ACGEXS_001976"
     CDS             5850..6245
                     /locus_tag="ACGEXS_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012973.1"
                     /translation="MLGKYWIQLLIATVIISLISIKAFPLAIGALYLPVIFKVIKLQL
                     NLSKGLIDDVNAQTFIKSNQSGIVISVICCLLITAILYYTLDGFYASLTGVLGTLVAL
                     NPYTTIVSAVLYILTAIATVEATKTKYRN"
     gene            6441..>7800
                     /gene="fumC"
                     /locus_tag="ACGEXS_001977"
     CDS             6441..>7800
                     /gene="fumC"
                     /locus_tag="ACGEXS_001977"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFF8012974.1"
                     /translation="MSVRIEHDTFGEIEVPADKYWGAQTERSKRNFPVGKERMPIEVV
                     YGFAQLKRAAAIANFDLGKLSEAKKDAIVYACDQILSGELDEHFPLVVWQTGSGTQSN
                     MNVNEVVSYVANMYLKDHQSDESIHPNDDVNKSQSSNDTFPTAMHVALYQEVETKLEP
                     ALKLLRNTLKEKEDKFDSIIKIGRTHLQDATPIKLGQEISGWRYMLDRCETMLSESKK
                     HILNLAIGGTAVGTGINAHPEFGDKVAHYISENTGYPFVSSENKFHALTAHDEVVQLH
                     GTLKALAGDLMKIANDVRWLASGPRAGLAEISIPENEPGSSIMPGKVNPTQCEMLTMV
                     AVQVMGNDTVVGFASSQGNFELNVYKPVIMHNTLQSIYLLADGMETFNNNCAVGIEPI
                     EENIDNYLNQSLMLVTALNPHIGYEKAAQIAKKAHKEGLTLKESAIQTGYVTEEQFEA
                     WIK"
BASE COUNT         2827 a         1054 c         1471 g         2448 t
ORIGIN      
        1 aggcgttact gtcaataaga tattgatagt agcgcttttt tgattgattt taaaatgtga
       61 gatttgttca cgtataatag cggatttttt atgtacagtc ttttaaaaag aagcttttct
      121 ttatattgat aaaaatacat ttataaaaca ctattgcatc aaaatgtatt aatatgtata
      181 attatgaata ttaattaggg agggctgtat aaatgaagtg tttaattagg tttatattag
      241 tattagggtt gttaattagc agtgctatgg tgtatatcaa tcccacagca catgctgaac
      301 aagatcaaac atgggagaag attaaagaac gcggtgaact tagagtgggt ctttctgcag
      361 attatgcacc aatggaattt gagcatacag ttaatggtaa gactgagtat gcaggtgtag
      421 atattgattt agctaaaaaa attgcgaaag ataataattt aaaattaaaa atcgtcaata
      481 tgtcatttga tagtttgtta ggagctctta aaactggaaa aattgatatt attatttccg
      541 gaatgacttc aacgcctgaa cgtaagaagc aagttgattt ttcagattca tatatgatga
      601 ctaaaaatat catgcttgta aagaaagata aagttaatga atataaagat atcaaagact
      661 ttaataataa aaaagtaggg gcacaaaagg gaactgaaca agaaaaaatc gctcaaaccg
      721 aaattgaaaa tgcatctatt acttcattaa gccgtttacc agacgttata ttagcattga
      781 aaagtggaaa ggttgaaggt gctgtagttg aaaaacctgt agcagaagca tatttaaaac
      841 aaaatcctaa attaggaatt tccaatgtga aatttaatga agaagaaaaa gatacagtga
      901 tagcagtgcc aaaagattca ccaaaattat tgtcacaaat taataaaacg attaaggagg
      961 ttaaagataa aggattaatc gataaatata tgactaatgc tgcaaatgcg atgaatgatg
     1021 acagtggttt tatttctaag tatggtagtt ttttcttgaa aggaattaag attacaatat
     1081 taatttcact tatcggtgtt gcattaggtt ctattttagg tgcattcgtt gcgttaatga
     1141 aattaagtaa aataaaaatt atttcatgga ttgcttctat ctatatcgaa atattaagag
     1201 gaacaccaat gttagttcaa gtatttatcg tattctttgg tataactgct gcattaggac
     1261 tagacatttc agctcttgtt tgtggaacaa ttgcattagt tattaattcc tcagcttaca
     1321 ttgctgaaat tattcgtgca ggtataaatg ctgttgataa aggccaaatg gaagccgcac
     1381 gtagtctagg tttgaattat agacaaacga tgaaaagtgt aattatgcca caagcaatta
     1441 aaaatatttt accagcttta ggtaatgaat ttgtcacttt aattaaagaa tcatctattg
     1501 tgtcaacaat tggagttggc gaaattatgt ttaatgcaca agtggttcaa ggtatttcat
     1561 ttgacccatt cacaccatta atagtggcag cagcattata ctttgtctta acatttgtac
     1621 ttacacgcat tatgaacatg attgaaggga gattgaatgc cagtgattaa aataaacaat
     1681 cttaataaag tttttggaga taatgaagtt ttaaaagata tcaatcttga aatcaatcaa
     1741 ggggaagtag tagcaataat aggtccatct ggtagtggta aaagtacatt gttaagatgt
     1801 atgaatttat tagaagtacc tactaaaggt caagtgattt ttgaaggcaa tgacttaacg
     1861 gaaaaaggga cacaagtaga taaactacgt caaaaaatgg gtatggtatt tcaaaacttc
     1921 aacctatttc cacataaaaa agttgtcgat aatattattt tagctcctaa attattaaag
     1981 aaagataata acgatgaatt acataaggaa gcattgtcgt tattagataa agtgggatta
     2041 aaagaaaaag cagatgtata tccgaatcaa ttatcaggtg gtcaaaaaca aagggtagca
     2101 attgcaagag ctttagcaat gcatccagat gttattttat tcgatgaacc aacttcagca
     2161 ttagatcctg aggtagttgg tgatgtatta aaagtaatga aagacctagc caaagaaggt
     2221 atgaccatgg tggttgtgac acatgaaatg ggatttgcca aagatgtaag tgacaaagtc
     2281 atatttatgg cagatggcgt tgtcgtagag tcaggcacac cagtcgaaat atttgaacaa
     2341 ccgcaacatg aaagaacaca aaatttctta gcaagagtat tataacaacc taacgaagct
     2401 tgaatatatg atacgcacca caaagttata tcatatattc aagcttttta ttattaaaat
     2461 tatgtgaaat gaaacctcaa tatgaataat ggttgtgtta aaataacata gtattatttt
     2521 atggggggca tacgattgga tacaaagcaa ttaaagcaag atatcattga ctatgcatat
     2581 acaattggta tcgacagtat tggatttact actgccgatc cctttgatga attgaagcaa
     2641 aagctagaag catatcatgc aaatggttat gcctcaggat ttgaagaatc tgatattgct
     2701 ttacgaacgg agcctaaatt atccttacca acagcaaggt caatcatagc aattgcagtt
     2761 ggttatccta acaaactgaa aggtgcacct aagagtgtta gaggtgatcg cagaggctta
     2821 tttgctagag catcgtgggg tcaagattat catacaatta tgcgtaaacg attagacatg
     2881 ttagctgcat ttattgaatc taaagttcca gatgttgaaa tcaaatctat ggtagatacg
     2941 ggtgtattat cagatagggc agtagcagaa cgtgcaggtt taggatttgt tggtcgtaat
     3001 ggctttgtca tcaatcctaa actaggaaca tggacatacc tcggtgaaat gttagtcagt
     3061 ataccttttg aacctgatga tccattatta gatagctgtg gtgattgtac aatttgtgtt
     3121 gatcgttgtc caacaagtgc attagttgga aatggccaat taaatagtca gaaatgcatt
     3181 agttttttaa cgcaaacaaa aggctatatg cctgaccaat atcgttataa aattgggaat
     3241 agattatatg gttgcgatac gtgtcaacaa gtttgtccga aaaatagagg tattaatacc
     3301 gaacaagatg acatcatttt ggaaccagaa attttaaagc caagattagt acctttacta
     3361 cgtatgtcta ataaagaatt caaacaaaca tatggtcacc ttgcaggtgc ttggcgtggt
     3421 aaaaagccga tacaacgaaa tgctatttta gcattggcgc attttaatga agtggatgca
     3481 attccagaat tgaaaaaagt tgcaacaaca gatgaaagac cgttgattcg agcaactgct
     3541 tattgggcca ttggtcaaat tcttggggaa gaagcaagag attttatcaa tgctaattat
     3601 gatcaagagg atgcagaagt tcagaatgaa atgataaaag gattagacac aaggagagaa
     3661 tagtaatatg acaaatcata tcgttttata tcaaccagaa atcccagcta atactggtaa
     3721 cattgcacgt acgtgtgcag gtacaaacac acatttacat ttaattaaac ctcttggatt
     3781 tagaactgat gataaaatgt taaaaagagc cggtttagat tattgggaat ttgtgaatat
     3841 tacgtatcat gatagtattg aagcattttt tgaagtcaca aatggcgagt attacttatt
     3901 aacaaaattt ggtaaaaaga cttatagtga ttttgatttt tcaaatcatg acaaagattt
     3961 ttacttcatt tttggaaaag aaacgacagg attaccagac tgggtgaaag agaaatatca
     4021 agacacagcg ttaagaattc ctatgagtga acatattcgt tcattaaatt tatcaaatac
     4081 tgctgcgttg ttaatttatg aagcgttacg tcaacaagat tttccgggat tgaattaaaa
     4141 tttactcatt ttcattataa aaatgatgct taagtgctat atttagagta taagcatgtt
     4201 aaagttaaat ggtttgatat attgaaagtt agggtataat aattattaat atcgccgttt
     4261 ttatgtaaaa tgcaaactaa gaggagtgta gaaatcatgg caatgaactt taaagtcttt
     4321 gacaatagtc aacttgtagc agaatatgct gctgatatta ttagaaagca atttaacaat
     4381 aatcctacta caattgcagg ttttcattta gatacagatc aagcgccagt tctagatgaa
     4441 ttaaagaaaa atgttgaaaa acatgctgtt gattttagcc aaataaatat tttagattat
     4501 gacgataaaa aatcatattt cgaagcgtta ggtgtaccag caggtcaagt ttatccaatt
     4561 gcttatgaaa aagatgcaat cgaattaatc gctgataaga ttaaaactaa agaaaataaa
     4621 gggaaattaa cattacaagt tgtttctatc gatgagcaag gtaagttaaa tgttagtatt
     4681 cgtcaaggac taatggaagc aagagaaatt ttcttagtag tgacaggtgc taataaacga
     4741 gatgtagttg aaaaattata tcaagaaaat ggtaaaacaa gcttcgaacc agccgattta
     4801 aaagcacata gaatggtaaa tgttattctt gataaagaag cggctgcagg tttacctgaa
     4861 gatgttaaag cttactttac gtcacgcttt gcttaattaa aaatctgtga ggtattgatt
     4921 atgtctaaag acaaagatcc aaaattaaat tatcatgaag aagaaaacag tatggtaacg
     4981 gattttgaag atttaaaaga attaggtaaa gaaatggaac aaatctctga tcaaaatgat
     5041 caagaaaaga attctgaaga agacagtcag taaagtatta taattgtgta atattcagtt
     5101 atttatcatg taaataatgt atagtataga acatatatca aataggattg attatcttga
     5161 atatgaagtt aggtagcatt ttgttaccta gcttttttat tatattcata gaagctgact
     5221 ttctgctagt ttcagtgttg gggctgtttc cccaacttac attgtttgta gaaattgagg
     5281 atccaatttt tctatgttgt ggccccgcca acttgcatgt tattttaagc tgactttcca
     5341 ccaacttctg agtttgtggc ccgtttccct aatttgcaat gtctgtagaa attagaaatc
     5401 cgatctcact attttggggc ctcgccaact tgcatgttat tttaagctga ctttccacca
     5461 gtttctgagt ttgtggcccg tttccccaat ttgcaatgtc tgtagaaatt gggaatccaa
     5521 ttccactatg ttggagctcc accaacttgc atgaaataga agctgacatt ccgaagcctc
     5581 tatgttgtgg actgcagtca actactgcca atataacttt gtaaagcata aaacattgat
     5641 ttgtgtacca gacatagtaa tatatcaaaa gttcaatttt aaatattaaa acaccattta
     5701 aacggaattg ataattaatt actccagaca taattgaaag aagtcatcta tcagtaatat
     5761 ttttaatgaa atcattaaac cgcgtttctt aatatgccaa gattatgata tgctattgaa
     5821 gaatgaaatt agaggagatt tatatattta tgttaggaaa atactggatt caattattga
     5881 tagcaacagt tattataagt ttaatatcaa ttaaagcttt tccattggca attggggcat
     5941 tatatttacc tgttattttc aaagtaataa aattacaact caatttatca aaaggattaa
     6001 ttgatgatgt taatgcgcaa acattcatta aaagtaatca atcaggtatt gtaatcagtg
     6061 taatttgttg tttattaata acagcaatat tgtattatac acttgatggg ttttatgcga
     6121 gtttaacagg agttttaggt acgcttgttg ctctaaatcc atatacgaca attgttagtg
     6181 ctgtattata tatacttaca gctattgcta cggttgaagc tactaaaaca aagtatcgta
     6241 attaaactta gttcaataat aactaaataa tgatagataa cacactaatc gtatgaccgt
     6301 atcactgtaa taaagggtga acacgtgcga ttagtgtatt tttatttcaa attatgaaaa
     6361 cgttttaatc ataaattgct caattaaatt atttaatgat atactacagt taaataaaca
     6421 gtgataaggg gagaaattga atgtcagtaa gaattgaaca tgatactttt ggagaaatag
     6481 aagtacctgc agataaatat tggggtgctc aaacagaaag aagtaaacgt aatttcccag
     6541 ttggtaaaga gcgtatgcca atcgaagtag tttatggttt tgcacaacta aagcgtgcag
     6601 cagcaatagc taattttgat ttaggaaaat taagcgaggc aaagaaagat gccattgtat
     6661 acgcatgtga tcaaatttta tcaggtgaat tagatgaaca cttcccacta gttgtatggc
     6721 aaacaggaag cggtacacaa agtaatatga atgtgaacga agtagtaagt tatgttgcta
     6781 atatgtattt aaaagatcat caaagtgatg aaagtatcca cccaaatgat gatgtaaata
     6841 aatctcaaag ttcgaatgat acattcccaa ctgctatgca cgttgcatta tatcaagagg
     6901 ttgaaacaaa attagaacct gcattaaaac ttttaagaaa tactttgaaa gaaaaagaag
     6961 ataaatttga ttcaattatt aaaattggtc gtacacattt acaagatgca acgccgatca
     7021 aactaggaca agagattagt ggctggcgtt atatgcttga ccgttgtgaa acaatgttat
     7081 ctgaatctaa gaagcacatt ttaaatcttg ccatcggtgg tacggctgtt ggtactggta
     7141 ttaatgcgca tcctgaattt ggtgataaag tggcacatta tatttcagaa aatacgggtt
     7201 atccatttgt atcttctgaa aataaattcc acgcacttac agcgcatgat gaagttgttc
     7261 aattgcatgg aacattgaag gcattagcag gagacttaat gaaaattgct aatgatgtga
     7321 gatggttggc ttcagggcca cgagctggtt tggcagaaat ttctatccct gaaaatgaac
     7381 caggttcatc aattatgcct ggtaaagtta atcctacaca atgtgaaatg ttaacaatgg
     7441 ttgcagtcca agtaatgggt aatgatacag ttgttggctt cgcaagttca caaggtaact
     7501 ttgaattgaa tgtttataaa ccagttatta tgcataatac actacaatca atttatcttt
     7561 tagctgatgg tatggaaaca tttaataaca attgtgcagt gggcattgaa ccaatcgaag
     7621 agaatattga taattattta aatcaatcat taatgttagt tactgcatta aatccacata
     7681 ttggttatga aaaagcagct caaattgcta agaaagccca taaagaaggt ttaactttaa
     7741 aagaatctgc aattcaaact ggatatgtta cagaagaaca atttgaagca tggattaaac
//
LOCUS       DAXWFK010000119         7781 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.119, whole genome shotgun sequence.
ACCESSION   DAXWFK010000119 DAXWFK010000000
VERSION     DAXWFK010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7781
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.119"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            121..782
                     /locus_tag="ACGEXS_001978"
                     /pseudo
     CDS             121..782
                     /locus_tag="ACGEXS_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1353..1754
                     /locus_tag="ACGEXS_001979"
     CDS             1353..1754
                     /locus_tag="ACGEXS_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFF8012975.1"
                     /translation="MKKVNNDTVFGILQLETLLGDINSIFSEIESEYKMSREEILILL
                     TLWQKGSMTLKEMDRFVEVKPYKRTRTYNNLVELEWIYKERPVDDERTVIIHFNEKLQ
                     QEKVELLNFISDAIASRATAMQNSLNAIIAV"
     gene            complement(1874..2437)
                     /locus_tag="ACGEXS_001980"
     CDS             complement(1874..2437)
                     /locus_tag="ACGEXS_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8012976.1"
                     /translation="MKLERILPFSKTLIKQHITPESIVVDATCGNGNDTLFLAEQVPE
                     GHVYGFDIQDLALENTRDKVKDFNHVSLIKDGHENIEHHINDAHKGHIDAAIFNLGYL
                     PKGDKSIVTKPDTTIQAINSLLSLMSIEGIIVLVIYHGHSEGQIEKHALLDYLSTLDQ
                     KHAQVLQYQFLNQRNHAPFICAIEKIS"
     gene            complement(2434..3387)
                     /locus_tag="ACGEXS_001981"
     CDS             complement(2434..3387)
                     /locus_tag="ACGEXS_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500380.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFF8012977.1"
                     /translation="MGNHFQYAFENKRYHTWNYHLKNKFGQKIFKVALDGGFDCPNRD
                     GTVAHGGCTFCSAAGSGDFAGNRADSIAVQFKEIKEKMHEKWHEGKYIAYFQAFTNTH
                     APVEVLKEKFEPVLKEPGVVGLSIGTRPDCLPDDVVEYLADLNQRTYLWVELGLQTIH
                     QSTSDLINRAHDMKTYYDGVAKLRKHNINVCTHIINGLPGEDYDMMMATAKEVAQMDV
                     QGIKIHLLHLLKGTPMVKQYDKGLLTFMTQEEYTNLVVDQLEVIPPEMIVHRITGDGP
                     IDIMVGPMWSVNKWEVLNGIDAELARRNSYQGLRYKSKVKQ"
     gene            3497..4678
                     /locus_tag="ACGEXS_001982"
     CDS             3497..4678
                     /locus_tag="ACGEXS_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFF8012978.1"
                     /translation="MNIPKSVWWLVIGMALNITGSSFLWPLNTIYMKQELGKSLTVAG
                     LVLMINSFGMVIGNLLGGSLFDKLGGYKTILIGTFTCLCSTTLLNFFHGWPWYAVWLV
                     MLGFGGGMIIPAIYAMAGAVWPNGGRQTFNAIYLAQNIGVAVGAAMGGFVAEFSFNYI
                     FLANLIMYVVFALVAVTQFNIEINAKVKYPTHLDITGKKNKARFISLVLICAMFAICW
                     VAYIQWESTIASFTQSINISMAQYSVLWTINGIMILVAQPLIKPILYLLKGNLKKQMF
                     VGIIIFMLSFFVTSFAENFTIFVVGMIILTFGEMFVWPAVPTIANQLAPDGKQGQYQG
                     FVNSAATVGKAFGPFLGGVLVDAFNMRMMFIGMMLLLVFALILLMVFKENNTQPKKID
                     A"
     gene            4966..7383
                     /gene="leuS"
                     /locus_tag="ACGEXS_001983"
     CDS             4966..7383
                     /gene="leuS"
                     /locus_tag="ACGEXS_001983"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFF8012979.1"
                     /translation="MLNYNHNQIEKKWQDYWDENKTFKTNDNLGQKKFYALDMFPYPS
                     GAGLHVGHPEGYTATDIISRYKRMQGYNVLHPMGWDAFGLPAEQYALDTGNDPREFTK
                     KNIQTFKRQIKELGFSYDWDREVNTTDPEYYKWTQWIFIQLYNKGLAYVDEVAVNWCP
                     ALGTVLSNEEVIDGVSERGGHPVYRKPMKQWVLKITEYADQLLADLDDLDWPESLKDM
                     QRNWIGRSEGAKVSFDVDNTEGKVEVFTTRPDTIYGASFLVLSPEHALVNSITTDEYK
                     EKVKAYQTEASKKSDLERTDLAKDKSGVFTGAYATNPLSGEKVQIWIADYVLSTYGTG
                     AIMAVPAHDDRDYEFAKKFDLPIIEVIEGGNVEEAAYTGEGKHINSGELDGLENEAAI
                     TKAIQLLEQKGAGEKKVNYKLRDWLFSRQRYWGEPIPVIHWEDGTMTTVPEEELPLLL
                     PETDEIKPSGTGESPLANIDSFVNVVDEKTGMKGRRETNTMPQWAGSCWYYLRYIDPK
                     NENMLADPEKLKHWLPVDLYIGGVEHAVLHLLYARFWHKVLYDLGIVPTKEPFQKLFN
                     QGMILGEGNEKMSKSKGNVINPDDIVQSHGADTLRLYEMFMGPLDAAIAWSEKGLDGS
                     RRFLDRVWRLMVNEDGTLSSKIVTTNNKSLDKVYNQTVKKVTEDFETLGFNTAISQLM
                     VFINECYKVDEVYKPYIEGFVKMLAPIAPHIGEELWSKLGHEESITYQPWPTYDEALL
                     VDDEVEIVVQVNGKLRAKIKIAKDTSKEEMQEIALSNDNVKASIEGKDIMKVIAVPQK
                     LVNIVAK"
     gene            7405..7716
                     /locus_tag="ACGEXS_001984"
     CDS             7405..7716
                     /locus_tag="ACGEXS_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFF8012980.1"
                     /translation="MKSITTDELKNKLLESKPVQIVDVRTDEETAMGYIPNAKLIPMD
                     TIPDNLNSFNKNEIYYIVCAGGVRSAKVVEYLEANGIDAVNVEGGMHAWGDEGLEIKS
                     I"
BASE COUNT         2665 a         1093 c         1444 g         2579 t
ORIGIN      
        1 aaaataagac atttttccca acttgcacat aaatgtaaaa ttcaataaaa tgaattttct
       61 gtgttgggtc ccttcgtata atttaataaa tactactaaa ctaaattaaa gaggtgcctt
      121 atgtataaaa attataacat gaccccaact tacactacca atagaaactt cgttagaatt
      181 cctcaaaatg atatttcgcg atatgttaat gaaattgttg aaacgatacc tgatagcgaa
      241 ttcgatgaat tcagacatca tcgtggtgca acatcctatc atccaaaaat gatgttaaaa
      301 atcatcttat atgcatatac tcaatctgta ttttctggtc gtagaataga gaaattactt
      361 catgacagta ttcgaatgat gtggttagcc caagatcaaa cactttctta taaaactatt
      421 aatcgtttta gagtgaatcc taatactgat gcgttaattg aatctttatt tattcagttc
      481 tatagtcaat gtttaaagca aaatcttatt gatgataatt caatttttat tgatggtaca
      541 aaagtagaag ctagtgccaa tagatataca ttggtgcgga agaaagtatt caaaatcacg
      601 aatcgaaatt gaacgagaat tcaaaaacac tatatcgtga cttagttgaa gaaaaaataa
      661 taccagagat aaaagaagat ggagatagcg atttaacaat agaagaaata gatttaattg
      721 gtagtcattt agataaagaa atcgaagatt gtgctcaaat tagaaaacag acccgtaaaa
      781 aagaactgaa agatgagaca gtagatgctc atcttttttt agaacttttt taaggttgaa
      841 aatgtatatc acattttata cacatttgat ttgtaagaaa tgttttgatt tatacaaatc
      901 atatcttgaa aaataaccaa tttagcctca ttcggtttga tttaatttgt taaatttaag
      961 gctaactaat taatttagtt taatcaattt tcatgagagt tatatgtaat aaaaattcaa
     1021 tgcgtatctt ttttgaagaa atatatgtag aattgttgca atttaatggt aatattgata
     1081 tattttcttt gtatataaat tataaaatta atatgtaata gagtgatttg ttttatgtac
     1141 tattatctta tttctaaata ttaactctat tgattattgg tttttatact tatttaattt
     1201 tattcaactt tgacaattga atagaaagca agtttattta cacttgtagt tttatgcata
     1261 agttagcaca tacaagtttt gggattgttg ggatgtttgt taatacttgt atagtagcta
     1321 aatatgtgat tattaattgg gagatgttta gcatgaaaaa agtaaataac gacactgtat
     1381 ttggaatttt gcaattagaa acacttttgg gtgacattaa ctcaattttc agcgagattg
     1441 aaagcgaata caaaatgtct agagaagaaa ttttaatttt actaacttta tggcaaaaag
     1501 gttctatgac gcttaaagaa atggacagat ttgttgaagt taaaccgtat aagcgtacga
     1561 gaacgtataa taatttagtt gaattagaat ggatttacaa agagcgtcct gttgacgatg
     1621 aaagaacagt tattattcat ttcaatgaaa agttacaaca agagaaagta gagttgttga
     1681 atttcatcag tgatgcgatt gcaagtagag caacagcaat gcaaaatagt ttaaacgcaa
     1741 ttattgctgt gtaagtttaa tagcataaaa agaggttttc attaagttga aaacctcttt
     1801 ttgttgttgg cattaatttt tcaaatgttg actactcaat cctaaattat aaatagtata
     1861 gcgcagcaaa tgcttaagaa attttttcta tggcacaaat gaatggagca tgattacgtt
     1921 ggtttaaaaa ttgatattgc aaaacttgcg catgcttttg atccaaagta ctcaagtaat
     1981 caagcaatgc atgcttctca atttgtcctt cgctatgacc atgatatata acaagtacaa
     2041 taataccttc aattgacatt aatgatagca atgaattaat agcttggatt gtcgtgtcag
     2101 gctttgtcac gatagattta tcacctttag gcaaataacc taggttaaag atggctgcat
     2161 caatatgacc tttatgtgca tcatttatat gatgttcaat attttcatgt ccatctttta
     2221 ttaaagaaac atgattgaaa tccttaactt tatcacgtgt attttccaaa gctaaatctt
     2281 gaatgtcgaa accataaaca tgtccttctg gtacttgttc ggctaaaaat aaagtgtcat
     2341 tgccgttacc gcaagttgcg tctacaacaa tactttctgg tgttatatgt tgtttaataa
     2401 gtgtttttga aaaagggagt atacgttcta atttcattgc ttcaccttag acttgtaacg
     2461 caagccttga taagaatttc tacgtgctaa ttcagcatcg atgccattta atacttccca
     2521 tttattaaca ctccacattg gacctaccat gatatctatt ggaccatcac cggtaattcg
     2581 gtgaacgatc atttcagggg gaatcacttc taattggtcc acaactaggt ttgtgtactc
     2641 ttcttgagtc ataaaagtta ataaaccttt atcgtattgt tttaccatcg gtgtaccttt
     2701 taacaaatga agtaaatgaa ttttaatacc ttgtacatcc atttgtgcaa cttctttggc
     2761 agtagccatc atcatgtcat agtcttcgcc aggtaagcca ttaatgatgt gtgtacatac
     2821 attgatatta tgcttacgta attttgccac accatcataa taagttttca tatcatgggc
     2881 acgattgatt aaatcagatg ttgactgatg gattgtttgt agtcctaatt caacccataa
     2941 gtatgttcgt tgattcaaat ctgctaaata ttcgacaaca tcgtctggta gacagtcagg
     3001 acgcgtacca atagataatc ccacaacacc cggttcttta agtacaggtt cgaatttttc
     3061 ttttaatact tcaaccggtg catgtgtatt tgtaaatgcc tgaaaataag caatatattt
     3121 tccttcgtgc catttctcat gcatcttttc tttaatttct ttaaattgta ctgcgattga
     3181 atctgcacga ttacctgcaa agtctccgct acctgcagca gaacaaaatg tacatccacc
     3241 atgtgctaca gtgccatcgc ggttaggaca gtcaaacccg ccatccaatg caactttaaa
     3301 tattttttgt ccaaatttat tttttaaatg gtaattccat gtgtgataac gtttgttttc
     3361 aaaagcgtat tggaaatgat tgcccatatg tcattttcct ttctataaaa aaagagttct
     3421 aagtacagat tttaacatat tttaatgtta tagtgtttat tatagtttga caaaaaagag
     3481 agaggaacta tgaaatatga atatacctaa atcagtctgg tggctagtaa ttggcatggc
     3541 gttaaatatt actggttcca gttttttgtg gcctttaaat acaatttata tgaaacaaga
     3601 acttggaaaa agtttaactg ttgctggttt agtgctaatg ataaattcat ttggcatggt
     3661 tattggaaac ttattaggtg gttcactatt tgataaatta ggtggataca agacgatttt
     3721 aattggaact ttcacttgtc tttgtagtac aacgctactt aatttctttc acgggtggcc
     3781 ttggtatgct gtatggcttg taatgttagg gtttggtggc ggaatgatta ttcctgcgat
     3841 atacgctatg gctggagcag tgtggccaaa tggcggaaga caaacgttta atgcgatata
     3901 cttagcgcaa aatattggtg tggctgtcgg tgctgcaatg ggcggctttg tcgcagaatt
     3961 tagctttaac tatatctttt tagccaatct tattatgtat gttgtgtttg cgcttgtcgc
     4021 ggtaacgcaa tttaatattg aaattaatgc gaaagttaaa tatccaactc atttagatat
     4081 tactggtaaa aagaataaag caagatttat ttcattagta ctaatttgtg caatgtttgc
     4141 aatttgttgg gttgcatata ttcaatggga gtctacaatc gcttcattta cacaatctat
     4201 taatatttca atggcacaat atagtgtttt atggacaatt aacggaataa tgattttagt
     4261 agcacaacca ttaattaaac cgattctcta tctgttaaaa ggaaacttaa agaagcaaat
     4321 gtttgtcggc atcatcattt ttatgttgtc gttctttgtc acgagttttg ccgaaaactt
     4381 tacaatattt gttgtcggta tgattatttt aacttttgga gaaatgtttg tatggccagc
     4441 agttccaact atagccaatc agttagcgcc agatggtaag caaggacagt accaaggttt
     4501 tgtgaattca gctgctacag taggaaaagc atttggtcca tttcttggtg gtgtattagt
     4561 tgatgcgttt aatatgcgca tgatgtttat cggtatgatg ctactacttg tatttgcatt
     4621 aatattatta atggttttca aggagaataa tacgcaacct aaaaaaatag atgcataatg
     4681 agtaaataga attaacgtta tagacttgaa ataaatgtcg ttataacata atattaattt
     4741 gtataattta atttcgtttg gagcttttct acagaaagct agtgatgctg agagctagtg
     4801 ttaaggacta aatgtaaatc gtattaattt taaattgaat gaatgacatc tcttactatt
     4861 aaaatgagtg cacaattttt gtgaaatagg gtggtaacgc ggcaaatgtc gtccctatgt
     4921 aaatagaata gttagaggtg tcttttttat tgaataggag gaaatgtgtt gaattacaac
     4981 cacaatcaaa ttgaaaagaa atggcaagac tattgggacg aaaataaaac atttaaaaca
     5041 aatgataact taggtcaaaa gaaattttat gctttagaca tgtttccata tccatcaggt
     5101 gctggtttac atgttggaca tcctgagggc tatacagcaa cagatatcat ttcaagatat
     5161 aaaagaatgc aaggatataa tgtattacat ccgatggggt gggatgcatt cggattacca
     5221 gcagagcaat atgctttaga cactggcaac gacccacgtg aatttacaaa gaaaaatatc
     5281 caaactttta aacgacaaat taaagaatta gggttcagtt atgattggga tcgtgaagtt
     5341 aatacaacag atccagaata ctataaatgg acacagtgga ttttcataca gttatataac
     5401 aaaggtttag catacgttga tgaagttgca gttaactggt gtccagcatt aggcactgtt
     5461 ttatctaacg aagaagtgat tgatggtgtc tctgaacgtg gtggacatcc agtttatcgt
     5521 aagccgatga aacaatgggt acttaaaatc acagaatatg cagatcaatt attagcagat
     5581 ttagatgatt tagattggcc tgagtcttta aaagatatgc agcgcaattg gattggacgt
     5641 tctgaaggag ccaaagtttc atttgatgta gataatacgg aaggaaaagt agaagtattt
     5701 acgactagac cagatacaat ctatggtgca tcattcttag tcttaagtcc tgaacatgca
     5761 ttagttaatt caattacaac agatgaatat aaagaaaaag taaaagctta tcaaacagaa
     5821 gcttctaaaa agtcagattt agaacgtaca gatttagcaa aagataaatc aggtgtattt
     5881 actggtgcat atgcaactaa tcctttatct ggtgaaaaag tacaaatttg gattgctgat
     5941 tatgtattat caacatatgg tactggagca attatggcag taccagcgca tgatgacaga
     6001 gattatgaat ttgctaaaaa gtttgatttg ccaatcattg aagtcatcga aggtggaaat
     6061 gttgaagaag cagcatacac tggtgaaggt aaacatatta attctggtga acttgatggt
     6121 ttagaaaatg aagcggcaat tactaaagct attcaattat tagagcaaaa aggtgctggc
     6181 gaaaagaaag ttaattacaa attaagagat tggttattca gtcgtcagcg ttattggggc
     6241 gaaccaattc ctgtcattca ttgggaagat ggaacaatga caactgttcc tgaagaagag
     6301 ctaccattgt tgttacctga aacagatgaa atcaagccat cagggactgg tgagtctcca
     6361 ctagctaata ttgattcatt tgtaaatgtt gtagatgaaa aaacaggtat gaaaggacgt
     6421 cgtgaaacaa atacaatgcc acaatgggca ggtagttgtt ggtattattt acgttacatc
     6481 gatcctaaaa atgaaaatat gttagcagat cctgaaaaat taaaacattg gttacctgtt
     6541 gatttatata tcggtggagt agaacatgcg gttcttcact tattatatgc aagattttgg
     6601 cataaagtcc tttatgattt gggtatcgta cctactaaag aacctttcca aaaattattt
     6661 aaccaaggta tgattttagg agaaggtaat gagaagatga gtaaatctaa aggaaatgta
     6721 atcaatcctg atgatatagt acagtctcat ggtgcagata ctttgcgtct ttacgaaatg
     6781 tttatgggac ctttagatgc tgcaattgca tggagtgaaa aaggattaga tgggtctcgt
     6841 cgattcttag atcgcgtatg gcgtttaatg gtaaatgaag atgggacatt gagttcaaaa
     6901 attgtaacta caaataataa atctttagat aaagtttata accaaactgt taaaaaggta
     6961 acagaagact ttgaaacatt aggatttaat actgctatta gtcaattaat ggtatttatt
     7021 aatgagtgtt ataaagttga tgaagtttat aaaccttaca ttgaaggctt cgttaaaatg
     7081 ttagcaccta ttgcaccaca tatcggtgaa gaattatggt caaaattagg acatgaagag
     7141 tctattacgt accaaccttg gccaacttat gacgaagcac tacttgtaga tgatgaagta
     7201 gaaatcgttg ttcaagtgaa tggtaaattg agagctaaaa ttaaaattgc taaagataca
     7261 tcaaaagaag aaatgcaaga aattgcctta tctaatgaca atgttaaagc gagtattgaa
     7321 ggtaaagaca tcatgaaagt catcgctgtt cctcaaaaat tagtcaatat tgtagctaaa
     7381 taatgtttta aggaggactt tgaaatgaag tcaattacta cagatgaatt aaaaaataaa
     7441 cttttagaat ctaaaccagt tcaaattgtt gatgttcgta ctgatgaaga aacagcaatg
     7501 ggatatattc ctaatgcaaa gttaattcca atggatacca ttccggataa tttaaattca
     7561 tttaataaaa atgaaatata ttatattgta tgtgctggtg gagttcgaag cgctaaagtt
     7621 gtagaatatt tagaggcaaa tggcattgat gccgtaaatg tcgaaggcgg catgcacgca
     7681 tggggcgatg aaggtttgga aataaaaagt atttaaagta gtgacataat ttaaaataat
     7741 attacatttg taatgacacc aagtaacgtt tcggttgctt g
//
LOCUS       DAXWFK010000120         7744 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.120, whole genome shotgun sequence.
ACCESSION   DAXWFK010000120 DAXWFK010000000
VERSION     DAXWFK010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7744
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.120"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            41..1021
                     /locus_tag="ACGEXS_001985"
     CDS             41..1021
                     /locus_tag="ACGEXS_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFF8012981.1"
                     /translation="MEDLERVLITGGAGFIGSHLVDDLQQDYDVYVLDNYRTGKRENI
                     KSLADDHVFELDIREYDAVEQIMKTYQFDYVIHLAALVSVAESVEKPILSQEINVVAT
                     LRLLEIIKKYNNHIKRFIFASSAAVYGDLPDLPKSDQSLILPLSPYAIDKYYGERTTL
                     NYCSLYNIPTAVVKFFNVFGPRQDPKSQYSGVISKMFDSFEHNKPFTFFGDGLQTRDF
                     VYVYDVVQSVRLIMEHKDAIGHGYNIGTGTFTNLLEVYRIIGELYGKSVEHEFKEARK
                     GDIKHSYADISNLKALGFVPKYTVETGLKDYFNFEVDNIEEVTAKEVEMS"
     gene            984..1676
                     /locus_tag="ACGEXS_001986"
     CDS             984..1676
                     /locus_tag="ACGEXS_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFF8012982.1"
                     /translation="MKKLQLKKWKCRENDIEAVHNNKGYAYQRKLDKLEEVRKSYYPI
                     KRAIDLILSIVLLFLTLPIMVIFAIAIVIDSPGNPIYSQVRVGKMGKLIKIYKLRSMC
                     KNAEKNGAQWADKDDDRITNVGKFIRKTRIDELPQLINVVKGEMSFIGPRPERPEFVE
                     LFSSEVIGFEQRCLVTPGLTGLAQIQGGYDLTPQQKLKYDMKYIHKGSLMMELYISIR
                     TLMVVITGEGSR"
     gene            1886..3052
                     /locus_tag="ACGEXS_001987"
     CDS             1886..3052
                     /locus_tag="ACGEXS_001987"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFF8012983.1"
                     /translation="MKIIYCITKADNGGAQTHLIQLANHFCVHNDVYVIVGNHGPMIE
                     QLDARVNVIIIEHLVGPIDFKQDILAVKVLAQLFSKIKPDVIHLHSSKAGTVGRIAKF
                     ISKSKDTRIVFTAHGWAFTEGVKPAKKFLYLVIEKLMSLITDSIICVSDFDKQLALKY
                     RFNRLKLTTIHNGIADVPAVKQTLKSQSHNNIGEVVGMLPNKQDLQINAPTKHQFVMI
                     ARFAYPKLPQNLIAAIEILKLHNSNHAHFTFIGDGPTLNDCQQQVVQAGLENDVTFLG
                     NVINASHLLSQYDTFILISKHEGLPISIIEAMATGLPVIASHVGGISELVADNGICMM
                     NNQPETIAKVLEKYLIDSDYIKMSNQSRKRYLECFTEEKMIKEVEDVYNGKSTQ"
     gene            3033..4271
                     /locus_tag="ACGEXS_001988"
     CDS             3033..4271
                     /locus_tag="ACGEXS_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFF8012984.1"
                     /translation="MENQHNSKLLTLLLIGLAVFIQQSSVIAGVNVSIADFITLLILV
                     YLLFFANHLLKANHFLQFFIILYTYRMIITLCLLFFDDLIFITVKEVLASTVKYAFVV
                     IYFYLGMIIFKLGNSKKVIVTSYIISSVTIGLFCIIAGLNKSPLLMKLLYFDEIRSKG
                     LMNDPNYFAMTQIITLVLAYKYIHNYIFKVLACGILLWSLTTTGSKTAFIILIVLAIY
                     FFIKKLFSRNAVSVVSMSVIMLILLCFTFYNINYYLFQLSDLDALPSLDRMASIFEEG
                     FASLNDSGSERSVVWINAISVIKYTLGFGVGLVDYVHIGSQINGILLVAHNTYLQIFA
                     EWGILFGALFIIFMLYLLFELFRFNISGKNVTAIVVMLTMLIYFLTVSFNNSRYVAFI
                     LGIIVFIVQYEKMERDRNEE"
     gene            4261..5691
                     /locus_tag="ACGEXS_001989"
     CDS             4261..5691
                     /locus_tag="ACGEXS_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFF8012985.1"
                     /translation="MKSDSLKENIIYQGLYQLIRTMTPLITIPIISRAFGPSGVGIVS
                     FSFNIVQYFLMIASVGVQLYFNRVIAKSVNDKRQLSQQFWDIFVSKLFLALTVFAMYM
                     VVITIFIDDYYLIFLLQGIYIIGAALDISWFYAGTEKFKIPSLSNIVASGIVLSVVVI
                     FVKDQSDLSLYVFTIAIVTVLNQLPLFIYLKRYISFVSVNWIHVWQLFRSSLAYLLPN
                     GQLNLYTSISCVVLGLVGTYQQVGIFSNAFNILTVAIIMINTFDLVMIPRITKMSIQQ
                     SHSLTKTLANNMNIQLILTIPMVFGLIAIMPSFYLWFFGEEFASTVPLMTILAILVLI
                     IPLNMLISRQYLLIVNKIRLYNASITIGAVINLVLCIILIYFYGIYGAAIARLITEFF
                     LLIWRFIDITKINVKLNIVSTIQCVIAAVMMFIVLGVVNHYLPPTMYATLLLIAIGIV
                     VYLLLMMTMKNQYVWQILRHLRHKTI"
     gene            5959..6558
                     /locus_tag="ACGEXS_001990"
     CDS             5959..6558
                     /locus_tag="ACGEXS_001990"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFF8012986.1"
                     /translation="MAFKLPNLPYAYDALEPYIDQRTMEFHHDKHHNTYVTKLNATVE
                     GTELEHQSLADMIANLDKVPEAMRMSVRNNGGGHFNHSLFWEILSPNSEEKGGVIDDI
                     KAQWGTLDEFKNEFANKATTLFGSGWTWLVVNDGKLEIVTTPNQDNPLTEGKTPILLF
                     DVWEHAYYLKYQNKRPDYMTAFWNIVNWKKVDELYQAAK"
     gene            6926..7527
                     /locus_tag="ACGEXS_001991"
                     /pseudo
     CDS             6926..7527
                     /locus_tag="ACGEXS_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498695.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7514..7651
                     /locus_tag="ACGEXS_001992"
     CDS             7514..7651
                     /locus_tag="ACGEXS_001992"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFF8012987.1"
                     /translation="MNQSNKKVLPAAGESMTSSILTASIAALLLVSGLFLAFRRRSTN
                     K"
BASE COUNT         2646 a         1085 c         1379 g         2634 t
ORIGIN      
        1 actttatgtt tttatgatat atttttaatg tataaatgag gtggaagatt tggaaagagt
       61 tttgataact ggtggggctg gttttattgg gtcgcattta gtagatgatt tacaacaaga
      121 ttatgatgtt tatgttctag ataactatag aacaggtaaa cgagaaaata ttaaaagttt
      181 ggctgacgat catgtgtttg aattagatat tcgtgaatat gatgcagttg aacaaatcat
      241 gaagacatat caatttgatt atgttattca tttagcagca ttagttagtg ttgctgagtc
      301 ggttgagaaa cctatcttat ctcaagaaat aaacgtcgta gcaacattaa gattgttaga
      361 aatcattaaa aaatataata atcatataaa acgttttatc tttgcttcgt cagcagctgt
      421 ttatggtgat cttcctgatt tgcctaaaag tgatcaatca ttaatcttac cattatcacc
      481 atatgcaata gataaatatt acggcgaacg gacgacatta aattattgtt cgttatataa
      541 cataccaaca gcggttgtta aattttttaa tgtatttggg ccaagacagg atcctaagtc
      601 acaatattca ggtgtgattt caaagatgtt cgattcattt gagcataaca agccatttac
      661 attttttggt gacggactgc aaactagaga ttttgtatat gtatatgatg ttgttcaatc
      721 tgtacgctta attatggaac acaaagatgc aattggacac ggttataaca ttggtacagg
      781 cacttttact aatttattag aggtttatcg tattattggt gaattatatg gaaaatcagt
      841 cgagcatgaa tttaaagaag cacgaaaagg agatattaag cattcttatg cagatatttc
      901 taacttaaag gcattaggat ttgttcctaa atatacagta gaaacaggtt taaaggatta
      961 ctttaatttt gaggtagata atattgaaga agttacagct aaagaagtgg aaatgtcgtg
     1021 aaaatgacat tgaagctgtc cataataata agggttatgc ctatcaaaga aaattagaca
     1081 aactagaaga agtgagaaaa agctattacc caattaaacg tgcgattgac ttaattttaa
     1141 gcattgtttt attattttta actttaccga ttatggttat attcgccatt gctatcgtca
     1201 tagattcgcc aggaaaccct atttatagtc aggttagagt tgggaagatg ggtaaattaa
     1261 ttaaaatata caaattacgt tcgatgtgca aaaacgcaga gaaaaacggt gcgcaatggg
     1321 ctgataaaga tgatgatcgt ataacaaatg tcgggaagtt tattcgtaaa acacgcattg
     1381 atgaattacc acaactaatt aatgttgtta aaggggaaat gagttttatt ggaccacgcc
     1441 cggaacgtcc ggaatttgta gaattattta gttcagaagt gataggtttc gagcaaagat
     1501 gtcttgttac accagggtta acaggacttg cgcaaattca aggtggatat gacttaacac
     1561 cgcaacaaaa actgaaatat gacatgaaat atatacataa aggtagttta atgatggaac
     1621 tatatatatc aattagaaca ttgatggttg ttattacagg ggaaggctca aggtagtctt
     1681 aatttactta ataagttcaa ataaaagtta tattttaaag attgtgacca attgttacag
     1741 tataacgagg aatcccttga gacagtatca aatggcatta agaaatatgt gccatcattg
     1801 atttgcatgg ctataaatac tattcatctg atgagatagc catgttaaga aattgaaagt
     1861 atagcattaa aggggtttgt aacagttgaa aattatatat tgtattacta aagcagacaa
     1921 tggtggtgca caaacacatc tcattcaact cgccaaccat ttttgcgtac acaatgatgt
     1981 ttatgtcatt gtaggcaatc atggaccaat gattgaacaa ctagatgcaa gagttaatgt
     2041 aattattatc gaacatttag taggtccaat tgactttaaa caagatattt tagctgtcaa
     2101 agtgttagca cagttattct cgaaaattaa acctgatgtt atccatttac attcttccaa
     2161 agctggaacg gtcggacgaa ttgcgaagtt catttcgaaa tcgaaagaca cacgtatagt
     2221 ttttactgca catggatggg cttttacaga gggtgttaaa ccagctaaaa aatttctata
     2281 tttagttatc gaaaaattaa tgtcacttat tacagatagc attatttgtg tttcagattt
     2341 cgataaacag ttagcgttaa aatatcgatt taatcgattg aaattaacca caatacataa
     2401 tggtattgca gatgttcccg ctgttaagca aacgctaaaa agccaatcac ataacaatat
     2461 tggcgaagta gttggaatgt tgcctaataa acaagattta cagattaatg ccccgacaaa
     2521 gcatcaattt gttatgattg caagatttgc ttatccaaaa ttgccacaaa atctaatcgc
     2581 ggcaatagag atattgaaat tacataacag taatcatgcg cattttacat ttataggcga
     2641 tggacctaca ttaaatgatt gtcagcaaca agttgtacaa gctgggttag aaaatgatgt
     2701 cacatttttg ggcaatgtca ttaatgcgag tcatttatta tcacaatacg atacgtttat
     2761 tttaataagt aagcatgaag gtttgccaat tagcattata gaagctatgg ctacaggttt
     2821 gcctgttata gccagtcatg ttggcggtat ttcagaatta gtagctgata atggtatatg
     2881 tatgatgaac aaccaacccg aaactattgc taaagtcctg gaaaaatatt taatagacag
     2941 tgattacatc aaaatgagta atcaatctag aaaacgttat ttagaatgtt ttactgagga
     3001 gaaaatgatt aaagaagtgg aagacgttta taatggaaaa tcaacacaat agtaaattac
     3061 taacattgtt acttatcggt ttagcggttt ttattcagca atcttcggtt attgccggtg
     3121 tgaatgtttc tatagctgac tttatcacat tactaatatt agtttattta ctgtttttcg
     3181 ctaaccattt attaaaggca aatcattttt tacagttttt cattattttg tatacatatc
     3241 gtatgattat tacgctttgt ttgctatttt ttgatgattt gatatttatt acggttaagg
     3301 aagttcttgc atctacagtt aaatatgcat ttgtagtcat ttatttctat ttagggatga
     3361 tcatctttaa gttaggtaat agcaaaaaag tgatcgttac ctcttatatt ataagcagtg
     3421 tgactatagg tctattttgt attatagctg gtttgaacaa gtccccttta ctaatgaaat
     3481 tgttatattt tgatgaaata cgttcaaaag gattaatgaa tgaccctaac tatttcgcga
     3541 tgacacagat tattacattg gtacttgctt acaagtatat tcataattac atattcaagg
     3601 tccttgcatg tggtattttg ctatggtctt taactacaac ggggtctaag actgcgttta
     3661 tcatattaat cgtcttagcc atttatttct ttattaaaaa gttatttagt agaaatgcgg
     3721 taagtgttgt gagtatgtca gtgattatgc tgatattact ttgttttacc ttttataata
     3781 tcaactacta tttattccaa ttaagcgacc ttgatgcctt accgtcatta gatcgaatgg
     3841 cgtctatttt tgaagagggc tttgcatcat taaatgatag tgggtctgag cgaagtgttg
     3901 tatggataaa tgccatttca gtaattaaat atacactagg ttttggtgtc ggattagtgg
     3961 attatgtaca tattggctcg caaattaatg gtattttact tgttgcccat aatacatatt
     4021 tgcagatctt tgcggaatgg ggcattttat tcggtgcatt atttatcata tttatgcttt
     4081 atttactgtt tgaattattt agatttaaca tttctgggaa aaatgtaaca gcaattgttg
     4141 taatgttgac gatgctgatt tactttttaa cagtatcatt taataactca agatatgtcg
     4201 cttttatttt aggaattatc gtctttattg ttcaatatga aaagatggaa agggatcgta
     4261 atgaagagtg attcactaaa agaaaatatt atttatcaag ggctatacca attgattaga
     4321 acgatgacac cactgattac aatacccatt atttcacgtg catttggtcc cagtggtgtg
     4381 ggtattgttt cattttcttt caatatcgtg caatactttt tgatgattgc aagtgttggc
     4441 gttcagttat attttaatag agttatcgcg aagtccgtta acgacaaacg gcaattgtca
     4501 cagcagtttt gggatatctt tgtcagtaaa ttatttttag cgttaacagt ttttgcgatg
     4561 tatatggtcg taattactat atttattgat gattactatc ttattttcct actacaagga
     4621 atctatatta taggtgcagc actcgatatt tcatggtttt atgctggaac tgaaaagttt
     4681 aaaattccta gcctcagtaa tattgttgcg tctggtattg tattaagtgt agttgttatt
     4741 tttgtcaaag atcaatcaga tttatcattg tatgtattta ctattgctat tgtgacggta
     4801 ttaaaccaat tacctttgtt tatctattta aaacgataca ttagctttgt ttcggttaat
     4861 tggatacacg tctggcaatt gtttcgttcg tcattagcat acttattacc aaatggacag
     4921 ctcaacttat atactagtat ttcttgcgtt gttcttggtt tagtaggtac ataccaacaa
     4981 gttggtatct tttctaacgc atttaatatt ttaacggtcg caatcataat gattaataca
     5041 tttgatcttg taatgattcc gcgtattacc aaaatgtcta tccagcaatc acatagttta
     5101 actaaaacgt tagctaataa tatgaatatt caattgatat taacaatacc tatggtcttt
     5161 ggtttaattg caattatgcc atcattttat ttatggttct ttggtgagga attcgcatca
     5221 actgtcccat tgatgaccat tttagcgata cttgtattaa tcattccttt aaatatgttg
     5281 ataagcaggc aatatttatt aatagtgaat aaaataagat tatataatgc gtcaattact
     5341 attggtgcag tgataaacct agtattatgt attattttga tatattttta tggaatttac
     5401 ggtgctgcta ttgcgcgttt aattacagag tttttcttgc tcatttggcg atttattgat
     5461 attactaaaa tcaatgtgaa gttgaatatt gtaagtacga ttcaatgtgt cattgctgct
     5521 gttatgatgt ttattgtgct tggtgtggtc aatcattatt tgccccctac aatgtacgct
     5581 acgctgctat taattgcgat tggtatagta gtttatcttt tattaatgat gactatgaaa
     5641 aatcaatacg tatggcaaat attgaggcat cttcgacata aaacaattta agtaccggta
     5701 atgctatact ttagaaaatt aagattaaga agaaaaggca atttcttatt gaaaaatgga
     5761 agttgtcttt tttaattctc tttaaaagcg ggaaacaaaa gcagttaaat gcctttttgc
     5821 attcaatatt aaatattata tcaatttcga atatttaaat tttatataat tggatataac
     5881 aaataaataa taattattgc aaaacacacc cgaaattaat tattataaaa gtatattcat
     5941 aaaaggagga atatacttat ggcatttaaa ttaccaaatt taccatatgc atatgatgca
     6001 ttggaaccat atatagatca aagaacaatg gagtttcatc acgacaaaca tcacaatacg
     6061 tacgtgacga aattaaacgc aacagttgaa ggaacagagt tagagcatca atcactagcg
     6121 gatatgattg ctaacttaga caaggtaccg gaagcgatga ggatgtcagt ccgtaataat
     6181 ggcggtggtc attttaacca ttcattattc tgggaaatac tatcacctaa ttctgaagaa
     6241 aaaggtggcg taatagatga catcaaagcg cagtggggca ctttagatga atttaaaaat
     6301 gaatttgcaa ataaagcaac aacattattt ggatcaggtt ggacttggtt agttgttaat
     6361 gatggcaaat tagaaattgt gacaacgcca aaccaagata atccattaac agaaggcaaa
     6421 acaccaatct tactatttga tgtttgggag catgcctact atctgaaata tcaaaataaa
     6481 cgtccagact atatgactgc attttggaac attgttaact ggaaaaaagt tgatgaatta
     6541 taccaagcag caaaataata taacttaata taattgaggt ggagcatcta caaggtgttc
     6601 cacttttttg tacttaatat ctttacttat ttgtttttgt gtaatgggat agcacgtaaa
     6661 agtggaaaaa taataagata aatttcaact cgaaatacct tattattgtt gactataacg
     6721 atgacctaaa tattatatac attagacaac actattaatt aatcaacata ttgacattaa
     6781 ataaattgac aaaataagta attattgtga atctaaagtg aaatttttat aaaaaaatgt
     6841 aatgattcaa aaattttgtt gcatttcttt tgtaatcgta tgataatgta aatgtaatca
     6901 aattgtaata taaggggaca agacaatgaa aaaattagca acagtaggtt ctttaattgt
     6961 aacaagcact ttagtattct caagtatgcc ttttcaaaat gcgcatgccg acacaacttc
     7021 aatgaatgtg tcgaataaac aaagccaaaa tgtacaaaat catcgtcctt atggcggagt
     7081 agtaccacaa ggaatgacgc aagcacaata tactgaatta gagaaagctt taccccaatt
     7141 aagcgctggc agtaatatgc aagactataa tatgaaattg tatgatgcga cgcaaaatat
     7201 tgctgataaa tacaatgtga taattacaac taatgtaggg gtatttaaac cacatgctgt
     7261 tagagatatg aatggccatg cgttaccttt aacaaaagat ggcaattttt atcaaacgaa
     7321 tgtagatgca aatggtgtta atcatggtgg tagtgaaatg gtgcaaaata aaacaggtca
     7381 tatgagtcaa caaggccata tgaatcagaa cacacacatg aaccaacagc cacacatgca
     7441 acaaggtcat atgcaatcat caaaccatca aatgatgagt ccaaaagcaa atatgcattc
     7501 atcaaatcat caaatgaacc aaagtaacaa aaaagtttta ccagctgctg gtgaaagtat
     7561 gacatcaagt attcttactg caagtattgc cgcactacta ttagtatctg ggttattctt
     7621 agcatttaga cgacgttcaa caaataaata aacataatac gattaataat agaaaaatcg
     7681 tgtgattatc tgagcgagcc taggacataa atcaatgtcc taggctcgct aatgttatat
     7741 tggc
//
LOCUS       DAXWFK010000121         7737 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.121, whole genome shotgun sequence.
ACCESSION   DAXWFK010000121 DAXWFK010000000
VERSION     DAXWFK010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7737
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.121"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..2194
                     /locus_tag="ACGEXS_001993"
     CDS             <1..2194
                     /locus_tag="ACGEXS_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HFF8012988.1"
                     /translation="LILAYEKTEIGFGEELYKDIIHHMKALFKCKNYLQIDTKIDMIN
                     NYLHQDIATNISEAAYLLWLLSRNNIGFTDLKVLHNRFIEKYGFEQLVNIKDLISDIT
                     GFGTTIFQEEETDGNNIVMLKQKFLHALRNNDEIVINEKDVESLINDNEINHYHAPMS
                     ADVYAELYLGRFYNQYNELIVISPLTASFNAGATFGRFHHLIDTETLAKLEHEKGHYY
                     QKMICDDNVEMISINNIPKYPRNHNVLTNHDSYEYSLNLGSSNSYSKYELTLDDIYVG
                     ATFNKLYLYSSQLNKRVLFESNNMYNFLKECNLYRLLREISMESVKCIEPMNDVSIDS
                     FSYSPRIRYKNVILKPAYWKINEMVLPLPKNEEWDQQFLKYQEQFNIPNIVNLVYGDN
                     KLLLNLSLANHRYLLMKEYKKHKRVRLVESFLPQSKNDHVYEIVTPIYKKSSYRGPEI
                     EIPKYKNTDIEYDKDWFALHIHIDKPSQDTFIIDNLYPFVKHLKDKGDIDQYFLMRYI
                     KQGDILKLRLFRNDENYAEIYSILKNWLPHVRQTTEVSDYEFVSYEPEFFRYGGKNTI
                     NEIEAFFEYDTNLAVNIIENDFKFDRPYIVAISIMYLFEMFSISNEERMEIVNNYVPT
                     SFKSKDIRPFKNELVTICNPANNFEYMAKHYSGIYRILKDGNQILSKLNEGLKKTLTT
                     KRSRIIGSLIHMRCNRIFGIDKDQETFVLSIVKEIVKTQKHWCGDKND"
     gene            2187..3431
                     /locus_tag="ACGEXS_001994"
     CDS             2187..3431
                     /locus_tag="ACGEXS_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase C family protein"
                     /protein_id="HFF8012989.1"
                     /translation="MIDMNLILKKKFQEISEVDDFISKASTETDYFEPSTLSHGIPGI
                     ILFLDAYQKVFDINTEQIVHKYIMKLAPYLQSGQYNNSLFGGLSGIAFSMDLASQNGK
                     NYQNILNNIDGHIVNEIENKFDQILQEPLNPLNYDTVSGLAGIGRYLLNRVDENEFNV
                     KALKSILVYFKDIQYSKNSWVVPQESQFLESDKNYFTEGNINLGLAHGVLGPMSLFAL
                     CVIKGITIENHQHILKDMYKFIMDEKFCNHERWLQRYDLISERNHFNFIRNGWCYGNT
                     GVMTTLFLIGQALQDDEIIKMSKKVMLQVVNDKDENLISPTICHGLSSQILMLTIMNL
                     NFELNEVSDYITVLINKLISHYKEDYLVNFIDINENKQDVFKSRKVGLLEGELGVYLT
                     LMTLKNTKILNEKNWTNAFLIS"
     gene            3447..3965
                     /locus_tag="ACGEXS_001995"
     CDS             3447..3965
                     /locus_tag="ACGEXS_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase family
                     flavoprotein"
                     /protein_id="HFF8012990.1"
                     /translation="MGENVLICLCGSVNSINISHYIIELKSKFDEVNVIASTNGRKFI
                     NGEILKQFCDNYYDEFEDPFLNHVDIANKHDKIIILPATSNTINKIANGICDNLLLTI
                     CHTAFEKLSIFPNMNLRMWENPVTQNNIRLLKDYGVSIYPANISESYELASKTFKKNV
                     VAPEPYKVLEFI"
     gene            3975..5348
                     /locus_tag="ACGEXS_001996"
     CDS             3975..5348
                     /locus_tag="ACGEXS_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HFF8012991.1"
                     /translation="MKIIKRAIISLIILSLLISITMSNASASEELYYSVEYKNTATFN
                     KLVKKKSLNVVYNIPELHVAQIKMTKMHANALANYKNDIKYINATCSTCITSEKTIDR
                     TSNESLFSRQWDMNKITNNGASYDDLPKHANTKIAIIDTGVMKNHDDLKNNFSTDSKN
                     LVPLNGFRGTEPEETGDVHDVNDRKGHGTMVSGQTSANGKLIGVAPNNKFTMYRVFGS
                     KKTELLWVSKAIVQAANDGNQVINISVGSYIILDKNDHQTFRKDEKVEYDALQKAINY
                     AKKKKSIVVAAAGNDGIDVNDKQKLKLQREYQGNGEVKDVPASMDNVVTVGSTDQKSN
                     LSEFSNFGMNYTDIAAPGGSFAYLNQFGVDKWMNEGYMHKENILTTANNGRYIYQAGT
                     SLATPKVSGALALIIDKYHLEKHPDKAIELLYQHGTSKNNKPFSRYGHGELDVYKALN
                     VANQKAS"
     gene            5371..6063
                     /locus_tag="ACGEXS_001997"
     CDS             5371..6063
                     /locus_tag="ACGEXS_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding subunit"
                     /protein_id="HFF8012992.1"
                     /translation="MAKLVTENISKRFKNQDVLKHINITLENNEVYGLLGINGAGKTT
                     LMKIICGILQQDSGEIKLDNRPMTRNDLHKVGSLIETPATYNHLSAQDNLKIVCLNES
                     VDFSEINSVLSLVNLNVDKKKKVKDFSLGMKQRLGIAMALIKKPEILVLDEPSNGLDP
                     YGIQELRELLKLLTEQGTSIIISSHILSEIQVLADHIGIIHEGELKYQQRNNKDENLE
                     EIFFKITKGDYK"
     gene            6060..6821
                     /locus_tag="ACGEXS_001998"
     CDS             6060..6821
                     /locus_tag="ACGEXS_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HFF8012993.1"
                     /translation="MIHLKIEGIKFKNSFSMYVLLISPLVFLCFAIFTVLFAKSNTGT
                     ANSVSPYITLLFNIWPIAFIPIVLCMACNSLFKIEMRNKSFNYYLSNNWSITKEIRAK
                     IFILSIAFLVHCFLVFIIAYIGDLIINPHPVNAMLLLVTILLMYVVSLPLIPLNFLLT
                     RYFGVSVSILINLVLSVICVLFLTLKSLFWVLPWGIMQRIPLITLGILPNGLVVNHNS
                     KYFNDLNALYISIIVSIIIFAIVTFLNNKKSWRLK"
     gene            6818..7516
                     /locus_tag="ACGEXS_001999"
     CDS             6818..7516
                     /locus_tag="ACGEXS_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsaG protein"
                     /protein_id="HFF8012994.1"
                     /translation="MIINELKSCKLKFSKQALTFVPIIVTILFILFINWYLNVNLWNG
                     RQISLFTASFNAITSLLISINVYQVINFEENIGHFNHILGKANRLNWLNASMIFTYTI
                     TAICILLASINLLWHSHDMKITLMFIGVSLFFNVIILLLLFIFSIFIKDVMAIVVGVL
                     MFIFNVYFGLEVLGDHSWFYLPITYATRYVYMFIEGSIPVTLTLAIYIIITLLLAVLL
                     FKGFNKWSGRTIKD"
BASE COUNT         2874 a          958 c         1313 g         2592 t
ORIGIN      
        1 attgatatta gcttatgaaa aaactgaaat tggatttggc gaggaattat ataaagacat
       61 tattcatcat atgaaggcac tattcaaatg taaaaactat cttcaaattg ataccaaaat
      121 tgacatgatt aacaattatt tacatcaaga tatagcaaca aatatatctg aagcagcgta
      181 cttattgtgg cttttatcaa gaaataatat tggattcaca gatttgaaag ttcttcacaa
      241 ccgttttatt gaaaagtatg gatttgaaca actcgtgaat attaaagatt tgatttcaga
      301 tataacaggt ttcgggacaa caatttttca agaagaagaa accgatggaa ataatattgt
      361 aatgcttaag caaaaatttt tacatgcctt gagaaataac gatgagatag tcataaatga
      421 aaaagatgta gagtcattga ttaacgataa tgaaatcaat cattatcatg cgccaatgag
      481 tgcagatgtt tatgctgaac tttatttagg acgtttttac aatcagtaca atgagctcat
      541 tgttattagt cctttaacgg cgtcttttaa tgctggcgct acatttgggc gatttcatca
      601 tttaattgat actgaaactt tagcaaaatt agaacatgaa aaaggacatt attatcagaa
      661 gatgatatgt gatgacaatg tagaaatgat ttctataaat aacataccga aatatccgag
      721 aaatcataat gtattaacta atcatgactc atacgaatat tcattgaatt taggaagtag
      781 taatagttat tcaaagtatg agcttacctt agatgatatt tatgttggtg ctacctttaa
      841 caaattatat ttatattcta gccaactaaa taaaagggta ctatttgaat caaacaatat
      901 gtataacttt ttaaaagaat gtaatttata tcgattgtta cgagaaattt caatggaatc
      961 tgtgaaatgc attgaaccta tgaatgatgt gagtattgat tcatttagtt attcaccacg
     1021 aatcagatat aaaaatgtca ttcttaaacc agcatattgg aaaattaacg agatggtatt
     1081 acctttacct aaaaatgaag aatgggatca acaatttttg aaatatcaag aacaattcaa
     1141 tatacctaat atcgtgaatt tagtttatgg agataacaag cttctactaa atttatcatt
     1201 agctaatcat agatatttat tgatgaaaga atataagaag cataaacgtg tacgtttagt
     1261 agaatcattt ttacctcaat caaagaatga tcatgtgtat gaaattgtta caccaattta
     1321 taaaaaatca tcttatcgag gacccgaaat cgaaattcca aaatataaaa atacagatat
     1381 tgaatatgac aaagactggt ttgcattaca tattcatatc gataagccat cgcaagatac
     1441 atttatcatc gacaatttgt atccatttgt aaaacatctt aaagataagg gagatataga
     1501 tcaatatttt ttaatgcgtt atatcaaaca aggtgacatt ttaaagttga ggttattcag
     1561 gaatgacgaa aattacgcag aaatatacag catcttgaag aattggttac cacatgttcg
     1621 acaaacgaca gaagtttcag attatgaatt tgtatcatac gagccagaat tttttagata
     1681 tggtggaaag aacacgatta atgaaataga agccttcttt gaatatgaca cgaatttagc
     1741 tgtcaatatt attgaaaatg attttaagtt tgatcgacca tatattgttg ctatttcaat
     1801 aatgtattta tttgagatgt tttcaatttc taatgaagag cgtatggaaa ttgttaataa
     1861 ctatgtacct acatcattta aaagtaaaga tattagacct tttaaaaatg aacttgtaac
     1921 tatatgtaat cctgctaata attttgaata tatggctaaa cactatagtg gtatttatcg
     1981 aattttaaaa gatggcaacc aaattttgag caaattaaat gaagggctta aaaaaacttt
     2041 aactactaaa aggtcaagga ttattggcag cttaattcat atgcgctgca atcgaatatt
     2101 tggcattgat aaggatcaag aaacatttgt tttatctatt gtaaaagaaa ttgttaaaac
     2161 tcaaaagcat tggtgtggtg acaaaaatga ttgatatgaa tctaatattg aagaagaagt
     2221 ttcaagagat ttcagaagta gatgatttca tttcaaaagc atctactgaa accgattatt
     2281 ttgaaccttc aactttatca catggtatac caggtatcat tttgttttta gatgcttatc
     2341 aaaaagtttt cgatattaat actgagcaaa ttgtgcataa gtacatcatg aaattagcac
     2401 catatttaca gagtggtcaa tataacaatt ctttgtttgg tggactttca ggtattgcat
     2461 tttcaatgga tttagcatcg caaaatggca aaaattatca aaatatttta aataatattg
     2521 atggtcacat tgtaaatgaa atagaaaata aatttgatca aatattacaa gaaccattaa
     2581 atccattaaa ttatgatact gtcagtggat tagctgggat agggagatat ttgctaaata
     2641 gagtagatga gaatgaattt aatgttaaag cattaaaaag catattagta tactttaaag
     2701 atattcaata ttctaaaaat agctgggtag tcccacaaga aagtcaattt ttagagtctg
     2761 ataaaaatta ttttactgaa ggtaatatca atcttggcct tgcacatgga gtgctaggac
     2821 cgatgtcttt atttgcatta tgcgtgatta aaggaattac gattgaaaat catcagcaca
     2881 tattaaaaga catgtacaaa tttatcatgg acgaaaaatt ttgtaaccac gaaagatggt
     2941 tgcagcgtta cgatttaatt tctgaacgta atcatttcaa ttttattcgg aatggttggt
     3001 gttatggcaa tacgggtgta atgacgacgt tgtttttaat cggccaagca ttacaagatg
     3061 atgaaataat taaaatgtct aaaaaagtga tgctacaagt agtaaatgat aaagatgaaa
     3121 atttaataag tccaactatt tgtcatggat tgtcatcaca aatattaatg ttaacaatta
     3181 tgaatttgaa ttttgaatta aatgaagtgt ctgattatat cactgtatta ataaataaac
     3241 tgatttctca ttataaggaa gattatctgg tgaattttat agacattaat gaaaataagc
     3301 aagatgtatt taaaagtagg aaagttggcc ttttagaagg tgaattaggg gtctatctaa
     3361 cattgatgac actgaaaaat acaaaaattc tcaatgaaaa aaattggacc aatgcgtttt
     3421 taattagctg aatggaggcg tatatcatgg gagaaaatgt attgatttgt ttgtgtggct
     3481 cagttaatag tatcaatatt agccactata ttattgaatt aaaatcaaaa tttgatgaag
     3541 taaatgtaat cgcgtcaaca aacggtagaa agtttattaa tggtgaaata ttaaagcaat
     3601 tttgcgataa ctattatgac gaatttgaag accctttttt aaatcatgta gatatagcaa
     3661 ataaacatga caagattatt attttacctg cgacttctaa tacgattaat aaaattgcaa
     3721 atggtatatg tgacaattta ttattaacta tttgtcatac agcttttgaa aaactttcta
     3781 tatttccaaa tatgaattta cgaatgtggg aaaatccagt tactcaaaat aatattcgat
     3841 tattaaaaga ttatggtgta tcaatatatc cagcaaatat ttcagaaagt tatgaattag
     3901 cgtcaaaaac atttaaaaag aatgttgtcg cacctgaacc atataaagtt ctggaattca
     3961 tttgagatta gaatatgaaa atcataaagc gcgctattat tagtttaata atattgagtc
     4021 ttttaatatc tattacaatg agtaatgcat cagcgtcaga agaactatat tacagtgttg
     4081 aatacaaaaa tacagcaaca tttaacaagt tagttaaaaa gaagtcctta aacgttgtct
     4141 ataatatacc ggaattacat gtggcacaga ttaaaatgac gaaaatgcat gctaatgctt
     4201 tagcaaacta taaaaatgat attaaatata tcaatgccac atgttcaact tgtattacta
     4261 gcgagaaaac aatagacaga acatcaaatg agtcattatt ttcaagacaa tgggatatga
     4321 ataaaataac caataatggt gcatcgtatg atgatttgcc aaaacatgct aacaccaaaa
     4381 tagcaatcat agatacaggt gtgatgaaaa accatgacga tttgaaaaat aatttctcga
     4441 ctgattctaa aaatttagta cctttaaacg gttttagagg tactgaaccg gaggaaacag
     4501 gtgatgttca cgatgtcaat gataggaaag gacatggcac gatggtgtcg ggtcaaacga
     4561 gtgctaatgg taagttaata ggtgttgcac cgaataacaa atttacaatg tatcgcgtgt
     4621 ttggtagtaa aaaaacagaa ctgctttggg tatcaaaagc gattgttcaa gctgcaaatg
     4681 atggaaatca agtcattaat attagtgttg gtagttatat tattttggac aaaaatgacc
     4741 atcaaacatt tagaaaagat gaaaaagtag aatacgatgc gttacagaaa gcaatcaatt
     4801 acgccaagaa gaaaaaatct atcgttgttg ctgcagctgg taatgatggt attgatgtca
     4861 atgacaaaca gaaactaaaa ttacagcgtg aatatcaagg taatggcgaa gtgaaagatg
     4921 ttcctgcatc tatggacaat gtcgttacag taggatctac agatcaaaag agtaatctat
     4981 ctgagttttc caattttggt atgaattata cagatattgc tgcgcccgga ggatcatttg
     5041 cttatttaaa tcaattcggt gtggataaat ggatgaatga agggtatatg cataaggaga
     5101 acattttaac tactgccaat aacggaagat atatttatca agctggaact tcattagcca
     5161 cacctaaagt ttcgggagca ctagctttaa tcattgataa atatcatctt gaaaaacatc
     5221 cagataaagc gattgaattg ttatatcagc atgggacatc taagaataat aaaccattta
     5281 gtagatatgg gcatggtgag cttgatgtgt ataaagcatt aaatgtagca aatcaaaaag
     5341 caagttaata aatcaaagga gtttttgatt atggcaaaat tagttactga aaacatttcg
     5401 aagcggttta aaaatcaaga tgtattaaag catattaata tcactttaga aaataacgaa
     5461 gtttatggat tacttggtat taatggagcc ggtaaaacga cacttatgaa aattatatgt
     5521 ggcatacttc aacaagattc aggggaaatt aaattagata atagaccaat gacacgaaat
     5581 gatttgcaca aagttggttc gcttattgaa acacctgcga catataatca tttaagtgca
     5641 caagataatt tgaaaattgt gtgtttaaat gaaagcgttg atttcagcga aattaatagt
     5701 gttttaagct tagtcaattt aaatgtcgat aaaaagaaaa aggttaagga cttttcttta
     5761 ggtatgaaac aaagacttgg aattgcaatg gcattaatta aaaagccaga aattttagta
     5821 ttagacgaac catctaatgg tttagaccca tatggaatcc aagaacttag agaacttcta
     5881 aaattattaa cagaacaagg tactagtatt attatttcaa gtcacatttt atctgaaatc
     5941 caagttttag cagatcatat cggtattatt catgagggtg agctaaaata tcagcaaaga
     6001 aataacaaag atgaaaactt agaagagata ttcttcaaaa taacgaaagg tgattacaaa
     6061 tgatacattt aaagatagaa ggtatcaaat ttaaaaattc tttcagtatg tatgttttat
     6121 taataagtcc gctggtattt ctttgttttg ctattttcac agtcttattc gccaaaagta
     6181 atacgggaac agcgaatagt gtgtcaccat atataacttt actatttaat atttggccaa
     6241 ttgctttcat cccgattgta ttatgtatgg cttgtaattc gttatttaaa attgaaatga
     6301 gaaataaatc atttaattat tacttaagta ataattggtc gattacaaaa gaaataagag
     6361 caaagatttt cattttatca atagcatttt tggtacattg ctttttagta tttattattg
     6421 cttatatagg tgacctaatt attaatccac atccggttaa tgctatgttg ttattggtga
     6481 caatattgtt gatgtatgta gtatctctac cattgatacc gctcaacttt ttattaactc
     6541 gatactttgg tgtgtccgta tcaatattaa taaacttagt attatcagtc atttgtgtct
     6601 tgtttttaac attgaagagt ttattttggg tgttgccgtg ggggataatg cagagaatcc
     6661 cgcttattac gcttggtata ctacctaatg gtttagttgt aaaccataat tcaaaatact
     6721 ttaatgatct caatgcctta tatatttcga ttattgttag catcattatt ttcgcgatag
     6781 taacattttt aaataataag aaaagttggc gattaaaatg ataattaacg aattaaagtc
     6841 atgtaagttg aaattttcta agcaagcgct cacatttgta cccattattg taaccatatt
     6901 gtttatatta tttataaatt ggtatttaaa cgtaaattta tggaatggtc gacaaataag
     6961 tttgtttaca gcgagtttta atgcaattac atcgctatta atttctataa acgtctatca
     7021 agttatcaat tttgaagaaa atattggtca ctttaatcat attttaggaa aagctaatag
     7081 gctaaattgg ttaaatgcat caatgatttt tacttatact attacagcca tatgtattct
     7141 attagcatca attaatttat tgtggcattc acatgatatg aaaataacac ttatgtttat
     7201 aggcgtatca ttgtttttca atgtaattat attactgcta ctttttattt ttagtatttt
     7261 cattaaagat gtaatggcta ttgttgtcgg agttttaatg tttattttta acgtttattt
     7321 tggattagaa gtgcttggag atcattcgtg gttctattta ccaatcacat atgctacacg
     7381 ttacgtttat atgtttatcg aagggagtat accagttaca ttaacattgg caatctatat
     7441 tattatcacg ttgctattag cagttttact ctttaaagga tttaataaat ggagcggtag
     7501 aacaattaaa gattaaagat tggggctcta acggtaaaag aaatttagtt gcgttgaaaa
     7561 gggaggaaag agaacagtta aatatgaata caaaataatt ttgttttcgg acagcagggg
     7621 tattagacgc gattgacaat gtctgtttaa ttaaacgtaa tgtttattta agcgatgaat
     7681 attaggtgaa aagtttttga atttgaatgt aattgaggtt tattgattag acatttt
//
LOCUS       DAXWFK010000122         7679 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.122, whole genome shotgun sequence.
ACCESSION   DAXWFK010000122 DAXWFK010000000
VERSION     DAXWFK010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7679
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.122"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(77..760)
                     /locus_tag="ACGEXS_002000"
     CDS             complement(77..760)
                     /locus_tag="ACGEXS_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFF8012995.1"
                     /translation="MSKSTKKSNDTIVETFKDIIPLSFGEEIGNAASHGVAALLTLLV
                     LPYAAVHSYIAHGTLASVSMSIYVISIFMMFISSTIYHSMQNETPHKYILRIIDHSMI
                     YVAISGTYTPILLTVVGGWIGWTVFILLWGTTLWGILYKSIAVKVNQKLSLIVYLVMG
                     WVGIILLPIIFIRTSWIFILFIFLGGVSYTIGAWFYAQKNRPYFHMIWHIFIVIASLL
                     HLIAILYFM"
     gene            complement(779..1966)
                     /locus_tag="ACGEXS_002001"
     CDS             complement(779..1966)
                     /locus_tag="ACGEXS_002001"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070569 - uridylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HFF8012996.1"
                     /translation="MLDKNQLAKYKQDHLCEYEKIMSNNEKEALEEKVASLDLDFIAK
                     LYNDLYINKKTIDDVSAVSEVKYDIKSQMSDDEIKRLEEQGLQAIKEGQFAVLLMAGG
                     QGTRLGYKGPKGSFEIEGVSLFELQANQLKTLNHQSGHTIQWYIMTSDINHEETLAYF
                     EAHSYFGYDQEAIHFFKQDNIVALSEEGKLILNQQGRIMETPNGNGGVFKSLDKAGYL
                     EEMSNNGVKYIFLNNIDNVLVRVLDPLFAGFTVEHDYDITSKTIQPKPGESVGRLVNV
                     DCKDTVLEYSELDPEVANQFNNANIGIHAFKLGFILNAVNRELPYHLAIKNLKQLDEN
                     FGVIEQPTLKFELFYFDIFTYGTSFVTLQVPREEEFSPLKNKEGKDSVATATEDLRRM
                     GLI"
     gene            complement(2010..2525)
                     /locus_tag="ACGEXS_002002"
     CDS             complement(2010..2525)
                     /locus_tag="ACGEXS_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFF8012997.1"
                     /translation="MTGKTHASCGMLVGALTTQYFQTDIFSSVTVIILATLASLLPDI
                     CHTQSKIGRRFKVISFFVRLIFGHRTFTHSILFIAIIAFLLQIIQTPNYYMAAIIIGL
                     VSHVILDMITPKGVKLFYPLPFNVKTPIQFKTGGLVDLSLATALMVGTVYVLFQPFIN
                     DIISNWNTKFL"
     gene            2702..2962
                     /locus_tag="ACGEXS_002003"
     CDS             2702..2962
                     /locus_tag="ACGEXS_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8012998.1"
                     /translation="MAMTVKKDNNEVRIQWRVADIKIPTSEIKNITQDQDIHAVPKLD
                     SKDVSRIGSTFGKTNRVIIDTEDHEYIIYTQNDQKVYNELTK"
     gene            complement(3094..4464)
                     /locus_tag="ACGEXS_002004"
     CDS             complement(3094..4464)
                     /locus_tag="ACGEXS_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HFF8012999.1"
                     /translation="MDSFSRKEIVIGRLKFITMSLIGILLFLVPIPVEQDGQKQTTLP
                     VAFLAGVLKDVLGGVMPFLIVTIITLSGIITLICSTILKDKLKPDGLMNNAFNVRIGW
                     LILRILAVVFAWMTFLRIGSKVIYSDETGGLLFSSLLPTLVAVFLFAALFLPLLMEYG
                     LLEMLGPIFRPVMRPLFTLPGRSTVDNLASFIGDGTVGVLITSRQYGEGYYSRREATV
                     ISTTFSVVSITFAIVVAETVHMQNQFFAFYLSVIVSCLVAAVIMPRIWPLNKIPDEYA
                     KEVPESARTEALPEGKTALRHGFDTATEVGIKAPGVIDFFKSGLKTVIDMWFVILPVV
                     MSIGTIATIIANYTPFFVILGKPFVPFLELMQIPEAAQASQTIIIGFADMFLPSILIE
                     GVQNDITRFVIGALSISQLIYLSEVGGVILGSKIPVSIGKLFMIFLIRTIITLPIISL
                     MAHLLL"
     gene            complement(4700..5668)
                     /locus_tag="ACGEXS_002005"
     CDS             complement(4700..5668)
                     /locus_tag="ACGEXS_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFF8013000.1"
                     /translation="MMTTKIIRRYSLITILLIVSIFISLCVGSVMIHPIDAIKGIFTQ
                     DDFILNEYRIPRTLLGIIIGSSLAISGAIIQAVIRNPLASPDVIGISKGASLAAVIII
                     MTFPTAPLFVLPIGSFLGAFAVSLFLSFLISKYDVKGSKLALIGLAIGAICTAIVQFL
                     LIRNPLEANKALVWLTGSLYGHDMSNIYTILPWFLITIPIIILLSYQLDVLNLGDAVA
                     TALGLKVKTIKMLLLILAVILAGSAISVVGGISFLGLIAPHIARQLIGNKTLHVIIMS
                     GLIGAILLTFGDGLARGIHPPLDIPVGVIIAIIGAPYFLILLRRMK"
     gene            complement(5665..6696)
                     /locus_tag="ACGEXS_002006"
     CDS             complement(5665..6696)
                     /locus_tag="ACGEXS_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFF8013001.1"
                     /translation="MAIKEISSQSAIDHKRKRRTTLTYIVSLCFLFICIYLNMAIGSS
                     KINFSDIIHYVTGHTDTKATFLLHNVRMPRMIAGLFIGGALAVSGLLMQAMTRNPLAS
                     PKIFGVSSGASFFIVFVTIIIPALEYYALYLGVIGAFIGGLTVYTLSGATKGMTPIKL
                     ALAGMAIHLFFSSMTEGIIILNENSNEQVMFWLVGSLSSMKWDEILTILPWIIGALIV
                     TIFIGRQLTIMELGDDIAKGLGQNINKVRIIIGLLVIILTGMSVSVAGPIGFVGLIVP
                     HIVKRYVSKNYLVMIPLTFIIGADLLLLSDVLSRLITYPYESPVGIVTSFVGALYFLF
                     ITIKGVKRI"
     gene            complement(6708..>7679)
                     /locus_tag="ACGEXS_002007"
     CDS             complement(6708..>7679)
                     /locus_tag="ACGEXS_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFF8013002.1"
                     /translation="KTFSILGLIVALLLVAACGNTDNSSKKESSTKDTISVKDENGTV
                     KVPKDAKRIVVLEYSFADALAALDVKPVGIADDGKKKRIIKPVREKIGDYTSVGTRKQ
                     PNLEEISKLKPDLIIADSSRHKGINKELNKIAPTLSLKSFDGDYKQNINSFKTIAKAL
                     NKEKEGEKRLAEHDKLINKYKDEIKFDRNQKVLPAVVAKAGLLAHPNYSYVGQFLNEL
                     GFKNALSDDVTKGLSKYLKGPYLQLDTEHLADLNPERMIIMTDHAKKDSAEFKKLQED
                     ATWKKLNAVKNNRVDIVDRDVWARSRGLISSEEMAKELVELSKKEQK"
BASE COUNT         2679 a         1446 c         1053 g         2501 t
ORIGIN      
        1 ctattacaat acaatcccct attagaatga tttatgtaat aaaaaaagcg gagtttcccc
       61 cagcttttct aaacgactac ataaaatata agattgcaat taaatgcaat agtgatgcta
      121 ttacaataaa aatatgccaa atcatatgaa aatatggtct attcttttgt gcataaaacc
      181 atgcaccaat tgtataagac acaccaccta agaaaatgaa taatatgaat atccatgatg
      241 tgcgaataaa aataattggt aacaagataa tacctaccca gcccatcact aaatagacaa
      301 ttaagcttaa cttttggtta actttgacag ctatggattt atataaaatc ccccaaagtg
      361 tcgttcccca taataaaata aatactgtcc aacctatcca accacctaca actgttaaca
      421 aaataggcgt atatgtacca gaaattgcta cgtaaatcat actgtgatcg ataatcctta
      481 aaatatattt atgaggtgtt tcattttgca tagaatgata aatagtagat gaaataaaca
      541 tcataaagat agaaataaca taaatagaca tactcacaga cgcaagcgta ccatgtgcaa
      601 tataactgtg cacagccgca tagggtaaca ctaataatgt taacaaagct gcaacaccat
      661 gagatgccgc attaccaatt tcttctccaa atgataaagg gatgatatcc ttaaatgttt
      721 ctacgattgt gtcattactt tttttagttg atttggacat catagtcacc gcctatcatt
      781 aaattaaacc cattctacgt aaatcttctg ttgcagttgc aacactatct ttaccttctt
      841 tatttttaag aggtgaaaat tcttcttctc taggaacttg taacgtgaca aaactagttc
      901 cataagtaaa tatgtcaaaa tagaacaatt cgaattttaa agttggttgt tcaataacac
      961 caaagttctc atctaattgc ttcaaattct taatagctaa atgataaggt aactcacgat
     1021 ttacagcgtt taaaataaaa ccaagtttaa atgcatgaat accgatatta gcattgttaa
     1081 attgatttgc aacttctgga tctaattctg aatactccag tactgtatct ttacaatcga
     1141 cattaactaa acgtccaaca ctttctcctg gttttggttg aatcgtttta gaagtaatat
     1201 cgtaatcatg ttctaccgta aatcctgcaa atagtggatc caacactctt actaaaacat
     1261 tgtcgatatt attcaagaaa atatacttaa cgccattatt agacatttct tctaagtatc
     1321 ctgccttgtc taaagattta aacacgccac cattaccatt cggcgtttcc ataatacgac
     1381 cctgttgatt taaaattaac ttcccttctt cactaagcgc cacaatatta tcttgtttaa
     1441 agaaatggat tgcttcttga tcatatccaa aataactatg tgcttcgaaa taagctaaag
     1501 tttcttcatg attgatatca cttgtcataa tataccattg aatcgtatgt ccagattgat
     1561 gattcaatgt ttttaattgg ttagcttgaa gttcaaataa acttacacct tcaatttcaa
     1621 acgaaccttt agggcccttg taaccaagtc ttgttccttg accacctgcc atcaaaagta
     1681 ctgcaaattg tccttcttta atagcttgaa gtccttgttc ctctaaacgc ttaatctcat
     1741 catcactcat ttgtgattta atatcatatt tcacttcaga aactgcagat acatcatcta
     1801 ttgttttctt attgatgtat aaatcgttat ataatttggc aataaaatct aaatctaatg
     1861 acgctacctt ctcttcaagt gcttcttttt cattgttact cattattttt tcatattcac
     1921 acaaatgatc ttgtttatat ttagctaatt gatttttatc tagcatttta tgcctcctgc
     1981 tgagtttgta acctttaata atttattttc tataaaaact tagtattcca gttgcttatt
     2041 atatcattga tgaaaggctg aaataaaaca taaactgttc cgaccattaa cgctgtagct
     2101 aaagataagt ctacaagtcc acctgtttta aattgaatcg gtgtcttcac attaaacggt
     2161 aacggataaa ataatttcac gccttttggt gttatcatat ctagtataac atgtgacact
     2221 aatccaatta taattgctgc catataataa ttaggcgtct gaataatttg caataaaaat
     2281 gctatgattg cgataaataa tattgaatgc gtaaaggttc gatgtccaaa gatcaatctc
     2341 acaaaaaaac taattacctt aaaccttctg ccaatcttac tttgagtgtg acatatatcg
     2401 ggtaataagc tagctagagt tgctagaatg ataaccgtaa ccgacgaaaa aatatccgtt
     2461 tgaaaatatt gtgttgttag cgctccaacg agcatgccgc atgaagcatg tgttttacct
     2521 gtcatatttg ttctccttta atactcacat tttaccacat ccctaacaaa aacacgaaca
     2581 tattttcggg ttaaaattca ttagtatgac acaatttaaa aaagtatcac ataactcttg
     2641 aaaacgatta caaaatcgtt tatgatgtat ttacaaaata tttaaaggat gtgtttgaat
     2701 aatggcaatg acagtaaaaa aggataataa tgaagtgcgt attcaatgga gagttgctga
     2761 tatcaaaatt cctacaagtg aaattaaaaa tattacacaa gaccaagata ttcatgcagt
     2821 tcctaaatta gacagcaaag atgtatctag aatcggctca acgtttggta aaacgaatcg
     2881 cgttattatc gatactgaag accacgaata cattatttat actcaaaatg atcaaaaagt
     2941 ttacaatgaa ttaactaaat aaattgtata aaaaaatcat tcatggtgag ggcttcatga
     3001 atgatttttt taattgattc aacacccagc ataaacaaat acaaaaggac aactgttccc
     3061 ataattttaa cagttgtcct ttttcacata tatttataac aaaagatgtg ccatcaaaga
     3121 aataattggt agtgtaatga ttgttctaat caagaaaatc ataaacaatt tgccgatgct
     3181 tacaggaatc ttcgaaccaa gtatgacgcc acctacttca gacaagtata ttaactgcga
     3241 tatactaagt gccccaataa caaaacgagt tatatcattt tgtacacctt caattaatat
     3301 agaaggtaaa aacatatcgg caaaaccgat aataatcgtt tgagaagcct gtgccgcttc
     3361 aggtatttgc attaactcta aaaatggaac aaaaggttta cccaatatga caaaaaaggg
     3421 cgtgtagttc gcaataatgg tagcaatagt accaatactc attactacag gcaaaataac
     3481 aaaccacata tcaatgactg tttttaatcc tgacttaaaa aagtcaataa cgcccggtgc
     3541 tttaatacct acttctgttg cagtatcaaa gccatgtctc aatgccgtct ttccttctgg
     3601 caatgcctca gtacgcgcac tttcaggtac ctccttagca tactcatcag gaattttatt
     3661 taaaggccaa attcttggca taatgactgc tgcaacgagg caggatacta tcactgataa
     3721 atagaaagca aaaaattgat tttgcatgtg cactgtttca gcaactacaa ttgcaaaggt
     3781 gatagaaact acactaaatg tcgttgaaat aactgttgcc tcacgacgag aataatatcc
     3841 ttcaccatat tgtctacttg taattaagac accaacagtt ccgtctccaa taaatgatgc
     3901 taaattatct accgtcgaac gtcctggcaa tgtaaataaa ggtctcataa ccggtctaaa
     3961 aataggaccc aacatctcta acaaaccgta ttccattaat agcggtaaaa atagagctgc
     4021 aaataagaat actgccacta atgttggcaa taaacttgag aacaataatc cacccgtttc
     4081 atctgagtaa ataacctttg aaccaattcg taaaaatgtc atccatgcaa aaacaactgc
     4141 taatattcgt aaaattaacc aaccaattct aacgttaaaa gcattgttca ttagcccgtc
     4201 aggtttcaat ttatctttta aaatagttga acaaatcaga gttatgatac ccgataaagt
     4261 aattatcgtc acaattaaaa atggcattac gccacctaat acatctttaa gcacgcctgc
     4321 taaaaatgcc acgggcaacg ttgtttgctt ctgtccatct tgttcgactg gaattggtac
     4381 taaaaataat aagataccaa ttaaagacat cgtaataaac ttaagtctcc caataactat
     4441 ctctttcctt gaaaagctat ccataaaatc aatccatttc tctatgtatt cgttttaagt
     4501 atatacagaa ttctattcag ttaacaaaca tattccttat cattctatct ttcaaaatgt
     4561 ttatgtatgc aaaataatga ataattacag ttattaaata tacgctattt cttgtaattt
     4621 ttcaagatga attcaaaaaa ggttaaatac aattactgat ttcgtactta acctttttta
     4681 aactctaatc atatgttagt tatttcattc ttcgtaataa tattaagaag tatggtgcac
     4741 cgataattgc aatgataacc ccaacaggaa tatccagtgg cggatgaatg ccacgggcta
     4801 aaccatctcc aaatgttaac aatatagcac caattaaccc cgacatgata ataacgtgta
     4861 atgttttatt tcctattaat tgtctcgcaa tatgaggtgc aattaatcct aaaaagctaa
     4921 taccaccgac aactgaaatt gcggatcctg ctaatattac tgctaaaatt aacaatagca
     4981 ttttaatagt tttaactttt aaaccgagtg cggttgcaac agcatcacct agattcaata
     5041 catctaattg ataactcaat aaaatgatga ttggtatcgt tattaaaaac caaggtaata
     5101 tagtataaat attcgacata tcatgtccat atagactacc tgtcaaccaa acaagcgctt
     5161 tgtttgcttc cagtggattt ctgattaata agaactgcac aatcgccgta catattgcgc
     5221 ctattgctaa accaattaag gcaagctttg aacctttaac atcatatttt gaaattaaaa
     5281 atgataaaaa taaacttact gcaaaggcac ctaagaatga acctataggt aatacaaaca
     5341 atggtgctgt tggaaaggtc ataataataa tcacagcagc taaactggca cctttagaaa
     5401 tacctataac atcaggtgag gctaacgggt ttcttattac agcttgtata attgcacctg
     5461 aaatagccaa gctactaccg ataataatac caagtaatgt tctaggtata cgatactcat
     5521 ttaaaataaa atcatcttgt gtaaagattc ccttaatagc atcaatcgga tgaatcatga
     5581 cagaccctac acataaactt atgaatatac tcacaattaa aaggattgtg attaaactat
     5641 aacgacgtat aattttcgtt gtcatcatat tcttttcacc cctttaatcg ttataaataa
     5701 gaagtaaagt gcacctacga atgatgtaac aatccctact ggtgattcat aaggatatgt
     5761 aattaaacga cttaatacat ctgatagtag taacaaatct gcacctataa taaatgttaa
     5821 tggaatcatg actaaataat tcttactcac atagcgtttg actatatgcg gtacgattaa
     5881 gccaacaaat ccaattggtc ctgccactga caccgacata cctgtaagaa taatgactaa
     5941 tagtccaatg ataattctaa ctttatttat attttgaccc aatcccttag caatgtcatc
     6001 gcctagttcc attatcgtta gttgacgtcc aataaaaatc gttacaatta aagcaccaat
     6061 aatccatggt agaatggtta aaatttcatc ccacttcata ctggataatg aaccgacaag
     6121 ccaaaacatc acttgttcgt ttgaattctc attcaaaata ataatacctt cggtcatact
     6181 actaaaaaac aaatggatgg ccataccagc caatgctaat ttaatgggtg tcataccttt
     6241 cgtagcacct gaaagtgtat aaacagttaa accacctata aatgcaccta tcacacctaa
     6301 atataatgca taatattcta atgctggaat gataatcgtc acaaatacaa tgaaaaacga
     6361 tgcaccagaa ctgacaccaa aaattttagg tgatgctagc ggattgcgtg tcattgcttg
     6421 cattaacaaa ccagataccg ctaatgcacc gccaataaat aaccctgcaa tcatccttgg
     6481 catacgtaca ttatgcaata aaaacgttgc tttcgtatct gtatgaccag taacatagtg
     6541 aatgatatcg ctaaaattaa ttttcgaaga accaatcgcc atatttaaat atatacaaat
     6601 aaaaagaaag cacaaactca ctatatacgt gagtgttgtg cgtctttttc ttttatgatc
     6661 tatggcagat tggctactta tttcttttat agccatttac ttccacctta cttttgttct
     6721 ttttttgata attcaacaag ttctttagcc atttcttcag aagaaattaa gccacgagat
     6781 cttgcccaaa catcacggtc aacaatatcc acgcgattat ttttaactgc attcaacttt
     6841 ttccatgttg catcttcttg taacttcttg aattcagcag aatctttttt agcatgatct
     6901 gtcataatga tcatacgctc tggatttaaa tcagctaaat gttcagtgtc taattgtaag
     6961 taaggtcctt tcaaatattt acttaaacct tttgttacat cgtcacttaa tgcattttta
     7021 aatcctagtt cgtttaaaaa ttgtccaaca tatgaatagt ttggatgtgc taataaacca
     7081 gctttagcaa ctactgctgg aagcactttt tgatttctat caaatttaat ttcatcttta
     7141 tacttattga ttaatttatc atgctcagca agacgttttt cgccttcttt ttctttattt
     7201 aaagctttag caattgtttt gaacgaatta atattttgtt tgtagtctcc atcaaaactc
     7261 tttaatgata atgttggtgc aattttgttt aattctttat taataccttt atgtctactg
     7321 ctatcagcga taattaaatc cggttttaat ttactaattt cttctaagtt tggctgttta
     7381 cgtgtaccta cagaagtata atccccaatt ttttctctaa ctggtttaat gatacgtttt
     7441 ttcttaccat catcagcaat accaactggt ttaacgtcta atgctgctaa tgcatctgca
     7501 aatgagtact ctaatacaac gatacgtttt gcatctttag gtacttttac tgtaccattt
     7561 tcatctttta ccgaaatagt atctttagtt gatgattctt ttttacttga attatccgta
     7621 ttaccacaag ctgcaactaa aagtaaggca actattaatc ccaatatact aaaagtttt
//
LOCUS       DAXWFK010000123         7660 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.123, whole genome shotgun sequence.
ACCESSION   DAXWFK010000123 DAXWFK010000000
VERSION     DAXWFK010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7660
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.123"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            311..3265
                     /gene="fdhF"
                     /locus_tag="ACGEXS_002008"
     CDS             311..3265
                     /gene="fdhF"
                     /locus_tag="ACGEXS_002008"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFF8013003.1"
                     /translation="MQEHLVVTLDGKDYLVEPGTNLLEFIKSQDTFVPSICYNESMGP
                     IQTCDTCTVEIDGKIERSCSTVIDRPMTVNTVNNDVKDAQKEALDRILEKHMLYCTVC
                     DYNNGDCEIHNTMDAWGLQHQTYEYKEKPYEKDYGPFYRYDPNQCILCGRCVEACQDI
                     EVNETIRIDWDREHPRVIWDNDVPINESSCVSCGQCATVCPCNAMMEVNMEGNAGYMT
                     DTEPGSLAAMIDLTKKAEPGYGPLFAISDSEAEMRKERIKKTKTVCTYCGVGCSFEVW
                     TKDREILKVQPSHDSPANKIATCVKGKFSWGHINSDQRLTKPLVRKNGEFHEVEWDEA
                     LNVIADNFTAIKEKHGPDALSFISSSKATNEESYLMQKLARQVIGTNNVDNCSRYCQA
                     PATKGLFRTVGHGGDSGSIEDLEKAAMSVLIGTNTAEAHPVIASRMKRAQKLFGQKIH
                     VFDIRKHEMAERADRFYQPKPGTDLAWLSAVTKYIIDHDLHDKAFIDEWVDDFDEYYK
                     SLETFTMAFAEEATGIPESELIKFAEECAKAESVVICWAMGITQQDIGSDSSTAISNL
                     LLVTGNYRRPGTGAYPLRGHNNVQGCSDMGSMPDKITGYQSIEADDIRAKFEKEYGVK
                     LNPKAGKDNHEMVEGIHDGEVHSLYLYGEDTGIVDSNINFVQAAFEKLDFMVVQDEFL
                     TFTATYADVVLPASPSLEKDGTFTNTERRIQRLYQALEPLGDSKPDWKIFQAIANRLG
                     FDWNYKHPSEIMDEVARLTPLYAGVSYDRLEGFNSLQWPVQPDGTDEPILYLEGFNFD
                     NGKAKLFPLSFDNYFKQDEIYDIHVNNGRLLEHFHEGNMTYQTPMIKYKVPRAFVEIS
                     PELAEDRGIHEGAEVKLISETGEAVLQVHVTDRVKGKEIYIPLNNDAMENGDLGAINL
                     LTNSDVDQYTDTPSYKRTSCRLEVITKRGKSPLNPNNFRVNKKRQPQYSVQVQKKWER
                     SDYVFPGNQVDK"
     gene            3265..3738
                     /locus_tag="ACGEXS_002009"
     CDS             3265..3738
                     /locus_tag="ACGEXS_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1641 domain-containing protein"
                     /protein_id="HFF8013004.1"
                     /translation="MAERISSKIRRLEKSEEQIKLESLNEVTEAIAANKDSILKAIKL
                     IKTLDDAKLLDALNGAIRGRQVIINKFAVELNKDIYTGLLSNMASMVFLLGELNVSDL
                     SDFLNKVNKGLHVANQASPNAKTTIRSLLGVLKDDDMNRSLTYMLNMLKGMSREE"
     gene            3966..4199
                     /locus_tag="ACGEXS_002010"
     CDS             3966..4199
                     /locus_tag="ACGEXS_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013005.1"
                     /translation="MLKTSAVISNTLLVIGLVCLFMMKLVLAITFFAVSLSISLVVFN
                     MMFRDRTTMKVIVNASFLIVILAIVVAYFLLSK"
     gene            4221..4997
                     /locus_tag="ACGEXS_002011"
     CDS             4221..4997
                     /locus_tag="ACGEXS_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HFF8013006.1"
                     /translation="MKKNFKLRISTLLLIVILVVFAVLLIVNETKLFKNDVNYSFDEA
                     VSMQQGKGIVQTKEEDGKFVEANNNEIAKAMTISHKDNDMKYMDITEKVPMSESEVNQ
                     LLKGKGILENRGKVFLEAQEKYEVNVIYLVSHALVETGNGKSELAKGIKDGKKRYYNF
                     FGIGAFDSSAVRSGKSYAEKEQWTSPDKAIIGGAKFIRNEYFENNQLNLYQMRWNPEN
                     PAQHQYASDIRWADKIAKLMDKSYKQFGIKKDDIRQTYYK"
     gene            5308..6432
                     /locus_tag="ACGEXS_002012"
     CDS             5308..6432
                     /locus_tag="ACGEXS_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HFF8013007.1"
                     /translation="MKIAIIGAGIGGLTAAALLQEQGHTIKVFEKNESVKEIGAGIGI
                     GDNVLKKLGNHDLAKGIKNAGQILSTMTVLDDKDRLLTTVKLKSNTLNVTLPRQTLID
                     IIKSYVKDDAIFTNHEVTHIDNETDKVTIHFAEQESEAFDLCIGADGIHSKVRQSVNA
                     DSKVLYQGYTCFRGLIDDIDLKHPDCAKEYWGRKGRVGIVPLLNNQAYWFITINSKEN
                     NHKYSSFGKPHLQAYFNHYPNEVREILDKQSETGILLHNIYDLKPLKSFVYGRTILLG
                     DAAHATTPNMGQGAGQAMEDAIVLVNCFNAYDFEKALQRYDKIRVKHTAKVIKRSRKI
                     GKIAQYRSRLFVAVRNRIMKMMPNALAAGQTKFLYKSKEK"
     gene            complement(6524..7477)
                     /locus_tag="ACGEXS_002013"
     CDS             complement(6524..7477)
                     /locus_tag="ACGEXS_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HFF8013008.1"
                     /translation="MEKVYVAGAIPEVGLKLLQEHFEVEMYEGKGLVDKDTLIKGVKN
                     ATALISLLSTNVDKDVIDAGKDLKIIANYGAGFNNIDIEYAREKSIDVTNTPKASTNA
                     TADLTIGLVLAVARRIVEGDQLSRTTGFDGWAPLFFRGREVSGKTIGIIGLGEIGSAV
                     ARRARAFDMDVLYTGPNRKEEKEREIGAKYVDLDTLLKNADFITINAAYNPKMHHLID
                     TEQFKMMKSTAYLINASRGPIVHEQALVQALKDNEIEGAALDVYEFEPDITDDLKSLN
                     NVVLTPHIGNATFEARDMMSKIVANAAISAVQGEKPQFVVN"
BASE COUNT         2726 a         1083 c         1473 g         2378 t
ORIGIN      
        1 gattattaaa tattgtcatt aataaggttg tgaaagatat ggcaaaaagc gtgaaacaga
       61 taattggaaa actaataatg ataattattt tcaattaaac aaaaagatga tttaatttcg
      121 cgcaaaatat ttcaaataaa aatgattgta aaaaggcaaa atacaaaatt ttacttaaca
      181 actagtacat aaagtaatac aattaaatta attctatctg aaagatgtgt ggggcatcgt
      241 tattttaggt ggatatgagc aatttattaa aagtcattta cggaaaatat atatagacgg
      301 ggtgagtaat atgcaagaac atttggtggt tacacttgat ggaaaagatt atcttgtaga
      361 accaggtacg aatttacttg aatttattaa atcacaagat acttttgtac cttcaatttg
      421 ttataacgag tcgatgggac caattcaaac atgtgataca tgtactgttg agattgacgg
      481 taaaattgaa cgttcatgta gtacggtgat tgatcgtcca atgactgtaa atactgtgaa
      541 caatgatgtg aaagatgctc aaaaagaagc gcttgatcga attttagaaa agcatatgct
      601 gtattgtaca gtatgtgatt ataataatgg tgattgtgaa attcataata cgatggatgc
      661 atgggggctt cagcatcaaa cgtatgaata taaagagaag ccatacgaaa aagattacgg
      721 cccattttat cgttatgatc caaatcaatg tatcttatgt gggcgttgtg tagaagcatg
      781 tcaagatatc gaagtgaatg aaactattag aattgattgg gatcgtgaac atccacgtgt
      841 tatttgggat aatgatgtac cgattaatga gtcttcatgt gtatcttgtg gtcaatgtgc
      901 gacggtatgt ccatgtaatg ccatgatgga agtgaatatg gaaggtaatg cgggttatat
      961 gactgatacg gaacctggtt cattagcagc aatgattgat ttaactaaaa aagcagaacc
     1021 tggttatggt ccactatttg caatctcaga ttcagaagca gaaatgcgta aagagcgtat
     1081 taagaaaact aaaacagtgt gtacttattg tggtgtgggt tgttcatttg aagtttggac
     1141 taaagataga gaaattttaa aagtacaacc atcacatgat tctccagcaa ataaaattgc
     1201 gacttgtgtt aaaggtaagt tttcatgggg acatattaat tcagatcaac gattaactaa
     1261 accactagta aggaaaaatg gtgagttcca tgaagtagaa tgggatgaag ctttaaacgt
     1321 cattgcagat aattttacag ctattaaaga aaagcatggc ccagatgcac tatcattcat
     1381 ttcttcttct aaagcgacga atgaagaatc gtatttaatg caaaaattag caagacaagt
     1441 tattggcaca aataacgttg ataactgttc aagatattgc caagcacctg caacaaaagg
     1501 cttatttaga acggttggac acggcggtga ctcaggtagt attgaagatt tagaaaaagc
     1561 ggcaatgtct gtattgatag gtactaatac agccgaagct catccagtta tcgcatcacg
     1621 catgaaacgt gcacaaaaat tatttggtca aaaaatacat gtatttgata ttagaaaaca
     1681 tgaaatggca gaacgtgcgg atcgttttta tcaacctaaa ccaggtacgg atttagcgtg
     1741 gttaagtgca gtgactaagt atattattga tcatgattta cacgataaag catttattga
     1801 tgagtgggta gatgattttg atgaatatta caaatcatta gaaacattta caatggcttt
     1861 tgctgaagaa gcaacaggta ttcctgaatc agaattgatt aaatttgctg aagaatgtgc
     1921 taaagctgaa tctgttgtaa tttgttgggc aatgggtatt acacaacaag acattggtag
     1981 tgactcaagt acagcgattt caaacttatt attagtaaca ggtaattatc gtcgtcctgg
     2041 tactggtgcg tatccattac gtggacataa taatgttcaa ggatgtagtg atatgggaag
     2101 tatgcctgat aagattactg gttatcaaag tattgaagcg gatgatattc gcgctaaatt
     2161 tgaaaaagaa tatggcgtta aattgaatcc aaaagctggt aaagataatc atgaaatggt
     2221 agaaggtata catgacggag aagtacactc attgtactta tatggtgaag atacgggtat
     2281 tgtggattca aatattaatt ttgtacaagc tgcgtttgaa aaattagatt tcatggtagt
     2341 ccaagatgaa tttttaacat tcacagcaac atacgcagat gttgtattgc cagcaagtcc
     2401 ttcacttgaa aaagacggta cttttacaaa taccgaacgt cgtattcaac gtttatacca
     2461 agcattagaa cctcttggtg attcaaaacc tgactggaaa attttccaag caattgctaa
     2521 tagattagga tttgattgga attacaagca tcctagtgaa attatggatg aagtagcacg
     2581 cttaacacct ctatatgctg gggtaagtta tgatcgttta gaaggattca atagtttaca
     2641 atggccagta caacctgatg gtactgatga gcctattcta tacttagaag gattcaattt
     2701 tgataatggt aaagcaaaat tattcccatt atcatttgat aattacttta agcaagatga
     2761 aatttatgat attcatgtaa acaatggtcg attgttagag cacttccatg aaggtaatat
     2821 gacttatcaa acaccaatga ttaaatacaa agtgccacgt gcatttgttg aaatttctcc
     2881 agagcttgct gaagatagag gcattcatga aggtgcagaa gttaagctga tttctgaaac
     2941 aggagaagcg gtattacaag ttcacgtgac agatcgtgtt aaaggtaaag aaatctatat
     3001 tccattaaat aatgatgcga tggaaaatgg agatctaggt gcaattaact tattaactaa
     3061 tagtgatgtt gatcaataca cggatacacc atcttataaa cgtactagct gtcgtttgga
     3121 ggtaattacg aaacgtggta agtcaccgtt gaaccctaat aatttccgtg tcaataaaaa
     3181 acgtcaaccg cagtacagtg ttcaagtaca gaaaaaatgg gaacgttcag attatgtttt
     3241 cccgggaaat caggtggata aataatggct gagagaatat cttctaaaat tagacgctta
     3301 gaaaaatcag aagaacaaat taagttagaa agtttaaatg aagttacaga ggcaattgca
     3361 gcgaataaag atagtatatt aaaagcaatc aagcttatta aaacattaga tgatgcaaaa
     3421 ctattagatg ctttaaatgg tgcaattcga ggacgtcaag tcatcataaa taaatttgca
     3481 gttgaactta ataaagatat ttatacaggg ctattatcta atatggcttc aatggtattt
     3541 ttattaggtg aactcaatgt atcagattta agcgacttcc taaataaagt taataaagga
     3601 ttacacgttg ccaatcaagc gagtccaaat gctaagacaa ctataagaag tctattgggc
     3661 gtattgaaag atgacgacat gaaccgtagt ttaacataca tgcttaatat gctaaaaggt
     3721 atgtctcgag aagaataaca ttacaatgaa aaagtgtttc tacatgcaag cagtgtagga
     3781 gcactttttt taatttttgt aaaaattaaa aagggtgttt ttgatggaat aaatgaataa
     3841 tattgaaaaa caaaaaaatc acgtgctatt gtgaatcaat catcgacagg cctatgaaac
     3901 atcatgtatt attatgatta tgtaagttat ttaaataaaa agggtgagtg catattaatt
     3961 cgagaatgtt aaaaacgtca gctgtaattt ctaatacatt attagtcata ggtttagtct
     4021 gtctatttat gatgaaatta gtgttagcta ttactttttt tgctgtttca ttatcaatta
     4081 gtctagttgt ctttaatatg atgttcagag atagaacaac gatgaaagta atcgttaacg
     4141 cttctttttt aatcgtaata ttagcgattg tagtggcata ttttttattg tctaagtaag
     4201 aaaaatgagg ttttgtctat atgaagaaga atttcaagtt acgcatttca acgctactat
     4261 tgatagttat tttagttgtt tttgctgtat tactcatcgt gaatgaaact aaattgttta
     4321 aaaatgatgt gaattactct tttgatgagg ctgtttcaat gcaacaaggg aaaggtattg
     4381 tacagacaaa agaagaggat ggtaaatttg ttgaagcaaa taataatgaa attgctaaag
     4441 caatgactat ttcacataaa gacaatgata tgaagtatat ggatatcaca gaaaaagtgc
     4501 caatgtcgga atctgaagtt aaccaattgc taaaaggtaa ggggatttta gaaaatcgag
     4561 ggaaagtttt tctagaagct caagaaaaat atgaggttaa tgtcatttat cttgttagcc
     4621 atgcattagt agaaacaggt aacggcaaat cagaattagc aaaaggcatt aaagatggga
     4681 aaaaacgcta ttacaacttt tttggtatag gagcattcga tagtagtgct gttcgtagtg
     4741 ggaaaagtta tgctgaaaag gaacaatgga catcaccaga taaggcgatt attggtggtg
     4801 caaagttcat tcgtaatgaa tattttgaaa acaatcaact gaatttatat caaatgcgat
     4861 ggaatccaga aaatcctgcg caacatcaat atgcgagtga cattcgctgg gcagataaaa
     4921 ttgccaaatt aatggataaa tcctataagc agtttggtat aaagaaagat gatattagac
     4981 aaacatatta taaataagac atcggtgctt aaaggagctg gaacaattta ttgtttcgag
     5041 ctcctttagc gcattctgag tgtgttagtt aaatggattt taacctaaca aaaaacgcta
     5101 tatagcatca aatatgctat atcccacatc attgttacaa atgtacatga tgtaaatgaa
     5161 tattgctgtc taaatgtgca tgtaatatac aatggtgcag ataatacact taagtcctta
     5221 aaaatgaaac gttagttcca agagtcattt ttaaacaata gtgcatgtga taaaatagaa
     5281 aagaatgaaa aatatagagg tgacaatatg aagatagcaa ttataggtgc aggcatcggt
     5341 ggattaacag ctgctgcatt attacaagaa caaggtcata ctattaaagt ctttgaaaaa
     5401 aatgagtcag ttaaagaaat tggcgctggg attggtatcg gagataatgt gcttaaaaaa
     5461 ctaggtaatc atgacttagc taaaggtatt aaaaatgctg ggcaaatctt atctacaatg
     5521 acagtgttag atgacaaaga tcgcctgtta actactgtta aattaaaaag taatacattg
     5581 aatgtgacgt taccacgcca aacattaatt gacattatta aatcttatgt aaaagatgac
     5641 gcaatattta caaatcatga agtcacgcat atagataatg agacagataa agttaccata
     5701 catttcgcgg aacaagaaag tgaagcattt gatttatgta ttggtgctga tggaattcat
     5761 tctaaagtga gacaatctgt aaatgctgac agtaaagtat tatatcaagg gtatacatgc
     5821 tttagaggtt taattgatga tattgattta aagcatccgg attgtgcaaa agaatactgg
     5881 ggaagaaaag gaagagtagg tattgttccg ttattaaata atcaagcata ttggttcatt
     5941 acaattaact cgaaggaaaa caatcataaa tatagttcgt ttggtaaacc tcatttgcaa
     6001 gcatacttta atcactatcc aaatgaagtt agagagatct tagacaaaca aagtgaaaca
     6061 ggtatcttat tgcataatat ttatgatttg aaaccactca aatcttttgt ttatggtcgt
     6121 actattttac taggagatgc agcacatgcg acaacgccta atatggggca aggtgctgga
     6181 caagcaatgg aagatgctat cgtattagta aattgtttta acgcatacga ctttgaaaaa
     6241 gcattacagc gttatgataa aatacgtgtc aaacatactg caaaagtaat taagcgttct
     6301 agaaaaatcg gtaaaattgc ccaatatcgt agtcgtttat ttgttgcagt tagaaatcgt
     6361 attatgaaaa tgatgccaaa tgcattagca gctggacaaa ctaaattctt atataaatcg
     6421 aaagaaaaat aatacaacaa tatgaaaacc cccgtatgtt gaaacgagag ctcaacatat
     6481 gggggttctt gtttttataa tgttattata ataaattcaa ttattagtta acgacaaatt
     6541 gtggtttctc accttgaacg gcactaattg cagcattagc aacaatttta gacatcatgt
     6601 cacgtgcttc aaatgtagca ttaccaatat gcggtgttaa tactacatta ttaagtgatt
     6661 ttaagtcatc ggtaatatct ggttcaaatt catatacatc aagtgcagca ccttcaattt
     6721 cattatcttt caatgcttgc actagtgctt gttcgtgcac gattggacca cgagaggcat
     6781 tgattaaata cgccgtagat ttcatcattt taaattgttc tgtatcaatt aaatgatgca
     6841 ttttaggatt ataagcagcg ttgatagtga taaaatctgc attctttaat agtgtatcta
     6901 aatctacata ttttgcaccg atttctcgtt ctttttcttc tttgcgatta ggtccagtgt
     6961 atagcacatc catgtcaaat gctcttgcac gacgagctac tgcactacca atttcaccta
     7021 aaccgataat gccgattgtt ttcccagata cttctctacc tctgaaaaat aaaggtgccc
     7081 atccatcaaa tccagttgta cgtgataatt ggtccccttc aacaatacga cgcgctactg
     7141 caagtactaa tccaattgtt aaatcagcag tcgcgtttgt tgatgcttta ggtgtgtttg
     7201 taacatctat acttttttct cgggcatact cgatatcaat attattaaaa ccagcgccat
     7261 agttggcaat gatttttaag tctttaccag catcgataac atctttatca acgtttgtag
     7321 ataataaact aattaaggca gtcgcgtttt taacaccttt aattaaagtg tctttatcga
     7381 ctaatccttt accttcatac atttcaactt caaaatgttc ttgtaaaagt tttaaaccta
     7441 cttctggtat tgcaccagca acataaactt tttccataaa agatcactcc ttttatctta
     7501 gtatagtaga agattagaca gtatacaact atgtcatgat gtcttgtgta tcaatgatgt
     7561 aagcgcgtac ttttgatgga ggcgatataa cttaggcact gtagaactat gaatattgta
     7621 atgtggaaaa actggatcaa ttaaattaga taacgtagtt
//
LOCUS       DAXWFK010000124         7595 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.124, whole genome shotgun sequence.
ACCESSION   DAXWFK010000124 DAXWFK010000000
VERSION     DAXWFK010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7595
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.124"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(59..376)
                     /locus_tag="ACGEXS_002014"
     CDS             complement(59..376)
                     /locus_tag="ACGEXS_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFF8013009.1"
                     /translation="MKSMVEMQREVDEYIGQFKTGYFSPLANLARLTEEVGELAREIN
                     HTYGEKKKKDSEEANTIKAELGDNLFVLLCLANSMGIDMTESFNETMEKFNTRDKNRF
                     ERK"
     gene            complement(713..1393)
                     /locus_tag="ACGEXS_002015"
     CDS             complement(713..1393)
                     /locus_tag="ACGEXS_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002449792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFF8013010.1"
                     /translation="MIIYFVILMVLPLWAQHKVKSNYEKYSQVRSTSGKTGREVALEI
                     LHANGIYDVEVVKGEGFLTDHYDPKKKVVSLSPANYDRPSVAGTAIAAHEVGHAIQDH
                     QGYWFLRFRAALVPVANLGSSLSYMIIMLGIILTAIGSAFGSTALWIGAGLMSLAVLF
                     SIVTLPVEFDASSRAMKQITALNIVNEKEYKHARKVLSAAAMTYVAATAVAVAELVRI
                     ILIARSSD"
     gene            complement(1465..2052)
                     /locus_tag="ACGEXS_002016"
     CDS             complement(1465..2052)
                     /locus_tag="ACGEXS_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1405 domain-containing protein"
                     /protein_id="HFF8013011.1"
                     /translation="MTIKAFWQYTLYQRSWLMMLLICNILGMIYGYIWYGEQLSHTPW
                     HFKIFVPDSPTAILYLVISISLILIQKQNSIIDALAFVTLFKYGIWAVIMNILFIIEQ
                     GDITVNGLVLMFSHSIMAVQAIYFYPRFKRSMIGISVAMIWVFLNDYIDYFHLQFPYY
                     DFITTHVWQIGVLSCCLSVFGLLLYIELNKLLKCK"
     gene            complement(2042..2624)
                     /locus_tag="ACGEXS_002017"
                     /pseudo
     CDS             complement(2042..2624)
                     /locus_tag="ACGEXS_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499997.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
     gene            complement(2638..3882)
                     /locus_tag="ACGEXS_002018"
     CDS             complement(2638..3882)
                     /locus_tag="ACGEXS_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFF8013012.1"
                     /translation="MEDIYKLIDDINLQKLENLDSRVNEAITTDNDDALFILGETLYN
                     FGLMPQGLEVFRVLYHKYPDESELLIYFIEGLMSENQTDEALEYLSYVEPSPEKLMLE
                     ADLYQQINMMEVAIDKLQEALELEPNDPIIHFALAEMLYYDGQYLRATSEYETVLETG
                     EYQVNGVNLFSRMADCSLQSGNYSDAIRLYDEINEDEMTSEDYLKKAISYDKNDITQE
                     AIKIMTTLLSKDPDYIQGYLYLQSLYENEKNYPDAIETGKEGLRLSQFYKELMYTTGC
                     LEIEHGDANEGVLLLKQALEVDNAYQEPLLILSDLYRNEEDYEAIIELLTYVDEEDLD
                     PTFMWHLAFAYGQEERDKEAQHFFELAYPTMKTNIDFMSDYYFYLIEIGQKEQAITIL
                     NQLLELEPSNENWHDELSRLQS"
     gene            complement(3889..5187)
                     /gene="aroA"
                     /locus_tag="ACGEXS_002019"
     CDS             complement(3889..5187)
                     /gene="aroA"
                     /locus_tag="ACGEXS_002019"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFF8013013.1"
                     /translation="MVNEQIIDISGPLKGEIEVPGDKSMTHRAIMLASLAEGVSTIYK
                     PLLGEDCRRTMDIFRLLGVEIKEDDEKLVVTSPGYQSFNTPHQVLYTGNSGTTTRLLA
                     GLLSGLGIESVLSGDVSIGKRPMDRVLRPLKLMDANIEGIEDNYTPLIIKPSVIKGIN
                     YQMEVASAQVKSAILFASLFSKEPTIIKELDVSRNHTETMFKHFNIPIEAEGLSINTT
                     PEAIRYIKPADFHVPGDISSAAFFIVAALITPGSDVTIHNVGINPTRSGIIDIVEKMG
                     GNIQLFNQTTGAEPTASIRIQYTPMLQPITIEGELVPKAIDELPVIALLCTQAVGTST
                     IKDAEELKVKETNRIDTTADMLNLLGFELQPTNDGLIIHPSEFKTNATVDSLTDHRIG
                     MMLAVASLLSSEPVKIKQFDAVNVSFPGFLPKLKLLENEG"
     gene            complement(5197..6261)
                     /gene="aroB"
                     /locus_tag="ACGEXS_002020"
     CDS             complement(5197..6261)
                     /gene="aroB"
                     /locus_tag="ACGEXS_002020"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFF8013014.1"
                     /translation="MKLQTTYPSNNYPIYVEHGAIDHISTYIDQFDQSFILIDEHVNQ
                     YFADKFDDILSYENVHKVIIPAGEKTKTFEQYQETLEYILSHHVTRNTAIIAVGGGAT
                     GDFAGFIAATLLRGVHFIQVPTTILAHDSSVGGKVGINSKQGKNLIGAFYRPTAVIYD
                     LDFLKTLPFEQILSGYAEVYKHALLNGESATQDIEQHFKDREILQSLNGMDKYIAKGI
                     ETKLDIVIADEKEQGVRKFLNLGHTFGHAVEYYHKIPHGHAVMVGIIYQFIVANALFD
                     SKHDINHYIQYLIQLGYPLDMITDLDFETLYQYMLSDKKNDKQGVQMVLIRQFGDIVV
                     QHVDQLTLQHACEQLKTYFK"
     gene            complement(6287..7453)
                     /gene="aroC"
                     /locus_tag="ACGEXS_002021"
     CDS             complement(6287..7453)
                     /gene="aroC"
                     /locus_tag="ACGEXS_002021"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFF8013015.1"
                     /translation="MRYLTSGESHGPQLTVIVEGVPANIEIKVEDINKEMFKRQGGYG
                     RGRRMQIEKDTVEIVSGVRNGYTLGSPITMVVTNDDFTHWRKIMGAAPISEEERENMK
                     RTITKPRPGHADLVGGMKYNHRDLRNVLERSSARETAARVAVGALCKVLLQQLDIDIY
                     SRVVEIGGIKDKDFYDSETFKANLDRNDVRVIDDSIAQAMRDKIDEAKNEGDSIGGVV
                     QVVVENMPVGVGSYVHYDRKLDGKIAQGVVSINAFKGVSFGEGFKAAEKPGSEIQDEI
                     LYNSEIGYYRGSNHLGGLEGGMSNGMPIIVNGVMKPIPTLYKPLNSVDINTKEDFKAT
                     IERSDSCAVPAASIVCEHVVAFEIAKALLEEFQSNHIEQLKQQIIERRQLNIEF"
BASE COUNT         2433 a         1434 c         1039 g         2689 t
ORIGIN      
        1 ttcattgatg gatgttttta atcttaatca aatccaaata aagcatatat ttttaaattc
       61 actttctttc gaatcgattt ttatctcttg tattaaactt ttccattgtt tcattaaagc
      121 tctctgtcat atctattccc attgaattcg ctaaacataa caacacaaat aaattatcac
      181 ctaattctgc tttaatcgta tttgcttcct ctgaatcttt cttctttttt tcaccatagg
      241 tatgatttat ttcacgtgca agttcgccca cttcttcagt caatctagct aagttagcta
      301 atggtgaaaa atatcctgtt ttaaattgtc caatatattc atcaacttca cgttgcattt
      361 ctaccattga tttcatttct acgttctcct tatattgcat ttctaatata gtatatatca
      421 atttgaagtc tcatgcatgt ttagtgcaat ctactgactc tagctaaact agatgacaag
      481 ttgtttattt tagaagttta ataattcagt ttatataaat gtaatgcatt cctaactaaa
      541 ttaaatcaat tgaaattggg attataactt tatgatacgt accactacaa taaaataata
      601 tagtgaataa tctaccatta gaaaaataag cacaaaaaaa ctagcaacca cacaaaaatg
      661 tgattagcta gttaataagt gtctaattta agttaattgt taatctataa gattaatcac
      721 ttgaacgcgc aatcaaaata atacgtacaa gctctgctac agcgactgca gttgctgcaa
      781 cataagtcat tgctgctgca gataatactt tacgcgcatg cttgtattct ttttcattta
      841 caatgttcaa tgccgtaatt tgtttcatcg ctcttgaact cgcatcaaac tcaactggta
      901 acgtaacaat tgagaataat accgctaatg acattaaacc agcaccaatc cataaagcag
      961 ttgaaccaaa tgcactacct atcgctgtta agataatacc taacatgatg atcatataac
     1021 ttaatgaact ccctaggttt gcaacaggta ctaatgctgc tctgaatctt aagaaccaat
     1081 atccttggtg atcttgaatg gcatgaccaa cttcgtgggc tgcaattgca gttccagcaa
     1141 ctgatggtct gtcatagttt gcaggagata gtgaaacaac tttcttttta ggatcgtaat
     1201 gatctgttaa gaatccttca cctttaacaa cttcgacatc ataaataccg tttgcatgta
     1261 aaatttctaa tgcaacttca cgacccgttt taccactagt tgatctaact tgtgaatatt
     1321 tctcatagtt agatttaact ttgtgttgtg cccataaagg aagcaccatt aatattacga
     1381 aataaattat catagtaaaa attgaagaca ataaactcac tctcctttat aaatatttta
     1441 ctgtcatttg ccgtttttat caaatcattt acactttaat aatttgttta attcaatata
     1501 aagcaaaagt ccaaaaacac ttagacaaca tgataataca ccaatttgcc acacatgtgt
     1561 agttataaaa tcataatatg gaaattgaag gtgaaaatag tcaatataat cattcaaaaa
     1621 cacccaaatc atcgctacac tgattccaat catagaacgt ttaaacctag gatagaagta
     1681 aattgcctga acagccatta tactgtggga aaacattaat accaaaccat ttactgtaat
     1741 atcaccttgt tcaataataa ataatatatt cattataact gcccaaatcc catatttgaa
     1801 taatgttaca aatgccagtg catcgataat actattttgt ttttgaatta atatcaatga
     1861 gatagaaata actaagtata atattgcagt tgggctatct ggaacaaaaa tcttaaaatg
     1921 ccagggcgta tgacttaatt gttcaccata ccatatataa ccataaatca tccctaatat
     1981 attacaaatg agtagcatca ttaaccaaga acgttgataa agtgtatatt gccaaaatgc
     2041 tttaattgtc atctgctaag tcctcaaatt gattatgttt atttactagc ttgagtgtat
     2101 ttaaaatttg cgttagttga taaaaacgtt gcttttcatt catctgtaaa cttaaatcaa
     2161 tattgtgtaa caagtaatct attaataacg catgtttatg ccgatctata gccatactat
     2221 ttaagtcatg aagataagtt tgataactgg gcgattccgt tagttgagca actattaaat
     2281 catctagcct ttggcctttg ctcacgcttt gacacgtttg cgaagtgaat ttgaatatca
     2341 aaagcacagt tatgattagc gatataatca aatatttcat ttgtattcat taactttata
     2401 ttacgcttag taaattgaat tgcagaagcg tgacttccca cttctgcaat ttctaatgtt
     2461 tcatgatgat taatttttgt atctacaaaa tgaatgtttg ccaatttcgc ctcattcact
     2521 tttatatagt taagcaccca aactgcaata cgcgacttaa atcgatattg aaaaagtaaa
     2581 tattcaataa aactttcttt aatttgattg agtgtctctg acatcaaata ccccatttta
     2641 agattgcaat cttgataatt cgtcatgcca attttcgtta cttggctcta gttccaacaa
     2701 ttgatttaaa atagtaattg cttgttcctt ttgaccaatt tcaattaaat agaaataata
     2761 atcactcata aaatcaatat ttgttttcat cgttggatat gctaattcaa agaaatgttg
     2821 agcttcttta tctcgctctt cttgaccata ggcgaacgct aaatgccaca tgaatgtagg
     2881 atccaaatct tcttcatcta catatgttaa taattcaatg attgcttcat aatcttcttc
     2941 attacgatat aaatcgctta aaatcaataa aggttcttgg taagcattat caacctctaa
     3001 tgcttgtttt aacaataata caccttcatt agcatcgccg tgttctattt ccaaacatcc
     3061 agttgtatac attaactctt tataaaattg acttagtcgt aatccttctt tacccgtttc
     3121 aatggcatct ggataatttt tttcattttc atataatgat tgtaaataca agtagccttg
     3181 aatataatca ggatctttag aaagtaatgt agtcattatt ttaattgctt cttgagtgat
     3241 gtcattttta tcgtaagaaa tggctttttt gagataatct tctgaagtca tttcatcttc
     3301 attaatttca tcgtataagc gaatcgcatc actatagtta ccactttgta aactacaatc
     3361 tgccatacga gagaataagt ttacaccatt aacttgatat tcaccagttt ctaaaacggt
     3421 ttcgtattca gaggtagcac gtaaatattg accatcataa tataacattt cagccaatgc
     3481 aaaatggatt attggatcat ttggctctag ttcaagtgct tcttgtaatt tatcaatagc
     3541 aacttccatc atattaattt gttgatataa atctgcttct aacatcaact tttcaggtga
     3601 tggttcaaca taacttaaat attctaacgc ttcgtcagtt tgattttcag acattaaacc
     3661 ttcaataaaa taaatcagca attcactttc gtctggatat ttgtgatata acacgcggaa
     3721 tacttccaaa ccttgtggca ttaatccaaa attgtaaagt gtctctccta gaataaataa
     3781 tgcgtcatcg ttgtcagtag ttattgcttc attaacacga gagtctaaat tttctagttt
     3841 ttgtagattg atatcgtcta ttaatttata gatatcttcc attttatatt atccctcatt
     3901 ttctaaaagc tttagttttg gtaaaaatcc tggaaatgat acatttacag catcaaattg
     3961 tttgattttg acaggctcgc ttgaaagtag agaagcaact gcaagcatca ttcctattcg
     4021 atgatcagtt aaactatcaa ctgttgcatt tgttttaaat tctgacggat gaataatcaa
     4081 tccatcatta gttggttgta attcaaaccc taacaagttt aacatatcag ccgttgtatc
     4141 aattctattt gtttctttta cttttaattc ctcggcatct ttaattgtac tcgtgccaac
     4201 tgcttgtgta caaagtaatg ctattacagg cagttcatca attgcttttg gaactaattc
     4261 tccttcgatt gttattggtt gaagcattgg tgtgtattga atacgaatag aagcagtagg
     4321 ttcagcacca gttgtttgat tgaaaagttg gatattaccg cccatttttt caacaatatc
     4381 aataatacct gaacgtgttg gattgattcc aacattatga attgttacat cacttcctgg
     4441 tgtgataagt gctgcaacaa taaagaacgc tgcagatgaa atatcgccag gaacatgaaa
     4501 atctgcaggt ttaatgtatc gaattgcttc aggggttgta ttaattgata acccttctgc
     4561 ttcaattgga atattaaaat gtttgaacat cgtctcagta tgatttcgac ttacatctaa
     4621 ttctttaatg atggtcggtt ccttagaaaa caaacttgca aataaaatgg cactttttac
     4681 ttgtgcactt gcaacttcca tttgataatt tatacctttt atgacagatg gcttaataat
     4741 taatggtgta taattatctt caataccttc aatattcgca tccataagtt tcaatggtct
     4801 caagacacga tccattggcc ttttaccaat tgaaacatcg ccagacaaaa cactttcaat
     4861 acctaaacca cttaacaaac ctgccaataa tcgtgttgtc gtaccagaat tacctgtata
     4921 caatacttga tgtggcgtgt taaaagattg atatcctggg gaagtcacaa ctaatttttc
     4981 atcatcttct ttgatttcta cacctaacag tcggaaaatg tccatcgtac gacgacaatc
     5041 ttcgccaagt agtggcttat atatagtaga tacaccttca gctagcgacg ccaacatgat
     5101 tgcacggtgt gtcattgact tatcgcccgg cacttctatt tcgcccttta acggacctga
     5161 aatatcaatg atttgttcat ttaccatttc attcacctac ttaaaatatg tttttaattg
     5221 ttcacatgca tgttgtaatg ttagttgatc aacatgttgt acaacgatat ctccaaattg
     5281 tctaatcaag accatttgta caccttgctt atcattcttt ttatcactta gcatatattg
     5341 gtataacgtt tcaaaatcca agtcagttat catgtctaaa ggatagccga gttgtattaa
     5401 atattgaata taatgattaa tatcatgctt agaatcaaac aaagcattcg caactataaa
     5461 ttgatagata atgccaacca tcactgcatg accatgaggt attttatgat agtattcaac
     5521 agcatgacca aatgtatgac ctaaatttaa aaatttacgt acaccttgtt ctttttcatc
     5581 tgcaataaca atatccagct tcgtttcaat acctttagca atatatttat ccataccatt
     5641 taatgactgt aatatctctc tatctttaaa gtgctgttcg atatcttgcg tcgctgattc
     5701 accattcaat aacgcatgct tataaacttc tgcatagcca cttaatattt gctcaaatgg
     5761 taacgtcttt aaaaagtcta aatcataaat cacagcagtt ggacgataaa atgcaccgat
     5821 aaggttttta ccttgctttg agttaatacc cactttaccg ccaacactag aatcatgcgc
     5881 tagtatagtc gttggcactt gtataaagtg aacgcctcgt aaaagtgtcg ccgcaataaa
     5941 cccagcaaaa tcaccagttg caccaccacc aacagcaata attgctgtat tacgagttac
     6001 atgatgggat aaaatatact ctaatgtttc ttgatattgc tcaaatgttt tcgtcttttc
     6061 accagctgga ataataactt tatgtacatt ttcatatgat aaaatatcat caaatttatc
     6121 agcaaaatat tgatttacat gctcgtcaat taatataaaa ctttgatcaa actgatcaat
     6181 atacgtgcta atatggtcaa ttgcaccgtg ttcaacatat attggataat tatttgaagg
     6241 gtatgttgtt tgtaatttca tgattacacc tcaattgttc ttgttgttaa aactcaatat
     6301 ttaattgtct gcgctcaata atttgttgtt taagttgctc aatatgattt gattggaatt
     6361 cttccaataa tgcttttgct atttcaaatg ctacgacatg ttcgcagacg atacttgctg
     6421 caggaacagc acaactatca gaacgttcaa ttgttgcttt aaagtcttct ttagtattaa
     6481 tgtctactga atttaatggt ttatataacg ttggaattgg tttcattaca ccattaacga
     6541 taattggcat tccatttgac ataccgcctt ctaaaccacc taagtgatta gatccacgat
     6601 aataaccaat ttcactatta tatagaattt catcttgaat ctcactacct ggcttttcag
     6661 ctgctttaaa tccttcacca aagcttacac ctttaaaagc atttatgctg acaacacctt
     6721 gtgcaatctt accatctaac ttacgatcat aatgcacata actacctaca ccaacaggca
     6781 tattttcaac tacaacttga acgacaccgc caattgaatc tccttcattt ttagcttcgt
     6841 caattttatc tcgcattgct tgtgcgatac tgtcatcaat tacacgaaca tcattacgat
     6901 caagatttgc tttaaatgtt tctgaatcat aaaaatcttt atctttaatt ccacctattt
     6961 caacaacacg actgtatata tcgatatcta actgttgtaa taacacttta cataaggcac
     7021 cgactgcaac tcgagctgct gtttctctag cagatgatcg ctctagcaca tttcgtaaat
     7081 cacgatgatt atatttcata cctccaacca aatctgcatg accaggtctt ggttttgtaa
     7141 tagtacgttt catattttca cgttcttctt cacttattgg agctgctccc ataatttttc
     7201 tccaatgcgt aaagtcatca ttggttacaa ccatagtaat tggactacct aatgtataac
     7261 catttctaac gcctgatact atttctactg tatctttctc aatttgcata cgacgtccac
     7321 gtccgtaacc gccttgacgc ttaaacattt ctttattaat atcctcaacc ttaatttcta
     7381 tatttgcagg tacaccttca acaataactg ttaattgagg tccatgtgat tctcctgatg
     7441 ttaggtatct catacacact cgctcctttc atacttccac gataacatta taatatgttg
     7501 ttaatcttat catattcatt gtgcattcat aaatgtttgt tttcaagctc aattaagaaa
     7561 atggatgaca tctcatttta aatgtcagaa ttatc
//
LOCUS       DAXWFK010000125         7272 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.125, whole genome shotgun sequence.
ACCESSION   DAXWFK010000125 DAXWFK010000000
VERSION     DAXWFK010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7272
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.125"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            99..1082
                     /locus_tag="ACGEXS_002022"
     CDS             99..1082
                     /locus_tag="ACGEXS_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFF8013016.1"
                     /translation="MKKWQFVGTTALGATLLLGACGGGNGGSGNSDLKGEAKGDGSST
                     VAPIVEKLNEKWAQDHSDAKISAGQAGTGAGFQKFIAGDIDFADASRPIKDEEKQKLQ
                     DKNIKYKEFKIAQDGVTVAVNKENDFVDELDKQQLKAIYSGKAKTWKDVNSKWPDKKI
                     NAVSPNSSHGTYDFFENEVMNKEDIKAEKNADTNAIVSSVTKNKEGIGYFGYNFYVQN
                     KDKLKEVKIKDENGKATEPTKKTIQDNSYALSRPLFIYVNEKALKDNKVMSEFIKFVL
                     EDKGKAAEEAGYVAAPEKTYKSQLDDLKAFIDKNQKSDDKKSDDKKSEDKK"
     gene            1273..2199
                     /gene="pstC"
                     /locus_tag="ACGEXS_002023"
     CDS             1273..2199
                     /gene="pstC"
                     /locus_tag="ACGEXS_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFF8013017.1"
                     /translation="MTSSTNVKALIEKNNNKKGKHNDKIIPVILAAISAISILTTLGI
                     LITLLLETITFFTRIPITEFLFSTTWNPTGSDPKFGIWALIIGTLKITVIATIFAVPV
                     GLGAAIYLSEYASDRARRIIKPILEILAGIPTIVFGFFALTFVTPVLRSFIPGLGEFN
                     AISPGLVVGIMIVPLITSLSEDAMASVPNKIREGAYGLGATKLEVATKVVLPAATSGI
                     VASIVLAISRAIGETMIVSLAAGSSPTASLSLTSSIQTMTGYIVEIATGDATFGSNIY
                     YSIYAVGFTLFIFTLIMNLLSQWISKRFREEY"
     gene            2201..3118
                     /gene="pstA"
                     /locus_tag="ACGEXS_002024"
     CDS             2201..3118
                     /gene="pstA"
                     /locus_tag="ACGEXS_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFF8013018.1"
                     /translation="METTDNNRQSLVDQQLVQKHLSSRTVKNKVFKLIFLACTLLGLV
                     VLIALLTQTLIKGVSHLNLQFFTNFSSSTPSMAGVKGALIGSLWLMLSIIPLSIILGI
                     GTAIYLEEYAKNNKFTQFVKISISNLAGVPSVVFGLLGYTLFVGGAGIEALKMGNSIL
                     AAALTMTLLILPIIIVSSQEAIRAVPNSVREASYGLGANKWQTIRRVVLPAALPGILT
                     GFILSLSRALGETAPLVLIGIPTILLATPRSILDQFSALPIQIFTWAKMPQEEFQNVA
                     SAGIIVLLVILILMNGVAIILRNKFSKKF"
     gene            3165..4016
                     /gene="pstB"
                     /locus_tag="ACGEXS_002025"
     CDS             3165..4016
                     /gene="pstB"
                     /locus_tag="ACGEXS_002025"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFF8013019.1"
                     /translation="MAQTLAQTKQISQSHTFDVSQSHHKTPDDTNSHSVIYSTQNLDL
                     WYGENHALQNINLDIYENQITAIIGPSGCGKSTYIKTLNRMVELVPSVKTAGKILYRD
                     QDIFDQKYSKEQLRTNVGMVFQQPNPFPKSIYDNITYGPKIHGIKNKKVLDEIVEKSL
                     RGAAIWDELKDRLHTNAYSLSGGQQQRVCIARCLAIEPEVILMDEPTSALDPISTLRV
                     EELVQELKEKYTIIMVTHNMQQAARVSDKTAFFLNGYVNEYDDTDKIFSNPSNKKTED
                     YISGRFG"
     gene            4023..4664
                     /gene="phoU"
                     /locus_tag="ACGEXS_002026"
     CDS             4023..4664
                     /gene="phoU"
                     /locus_tag="ACGEXS_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFF8013020.1"
                     /translation="MAIIRQRYQEQLDDLIKELRRLGANVYVSIENGIKSLSIDDRGF
                     ARQTVKNDKHINQLNYDINERVIMLITKQQPIASDLRMMISSLKIASDLERIGDNASS
                     IANIRLRTKITDDYVLTRLKTMGKLAMLMLKDLDQAFKKKDTVLIREIIERDEDIDDL
                     YSHIINATYLIDNDPFVAAQAHLAARHLERIGDHIINIAESVYFYLTGTHYEQ"
     gene            complement(4804..6618)
                     /gene="pepF"
                     /locus_tag="ACGEXS_002027"
     CDS             complement(4804..6618)
                     /gene="pepF"
                     /locus_tag="ACGEXS_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFF8013021.1"
                     /translation="MSQGLPLREDVPVSETWDLVDLFKDDQQYYESIDALVQQANQFH
                     HTYATTLNSIEQINTALAELENILIALDRLSNYAELRLSVDTSNIEAQVLSAKLSTTY
                     GKIVSQLSFVESEILELPEEILQQLEESCPYQHYIKQLIKQKPFQLSASVEQVLATLS
                     PTLNSPYDLYGTTKMLDITFDSFEHDGTTYPVDYATFENDYEDNKDPEFRRKSFKSFS
                     DGIRKYQHTTAATYNMQVQQEKIEADLRGFESVIDYLLHSQEVTRDMFDRQIDMIMRD
                     LAPVMQKYAKLLQRIHGLDNMRFEDLKISVDPDYEPEISIEDSKNYIFGALSVLGDDY
                     TNMLREAYDQRWIDFAQNKGKDTGAFCASPYFTHSYVFISWTGKMAEAFVLAHELGHA
                     GHFTLAQKHQPYLESEASMYFVEAPSTMNEMLMANYLFNTSDNPRFKRWVIGSILSRT
                     YYHNMVTHLLEAAYQREVYHKVDQGESLNAPTLNEIMLNVYKQFFGDAVDMTEGAELT
                     WMRQPHYYMGLYSYTYSAGLTIGTVVSQKIKNEGQPAVDAWLETLKKGGSVSPVELAN
                     IAGVDITTEQPLKSTIQYISDLVDEVEKLTDEIEQANN"
     gene            6823..7167
                     /locus_tag="ACGEXS_002028"
     CDS             6823..7167
                     /locus_tag="ACGEXS_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013022.1"
                     /translation="MTLFDMPNYLWITTLIMILLTIFCCLVLNKWFVSAVITFVILGV
                     LAFFIPNFQDIKYQPLLGYAAFLAIMSLLISFLLWYFTRNWRKERKARKLEKQIEKYG
                     YEGAELRRKDKK"
     gene            7115..>7272
                     /locus_tag="ACGEXS_002029"
     CDS             7115..>7272
                     /locus_tag="ACGEXS_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013023.1"
                     /translation="MKNTAMRALNFAVKIKNNIRQLYAQNHRLLSYTAVTIISKEMEY
                     SAIVVFVLT"
BASE COUNT         2544 a         1124 c         1239 g         2365 t
ORIGIN      
        1 atttacagtt tctaaaaatg aggttaataa ttcaaggtta agataaagat gtaatcaata
       61 caaatactat ttgttgttca tacagggagg atatttcaat gaaaaaatgg caatttgttg
      121 gtactacagc tttaggtgca acactattat taggtgcttg tggtggcggt aatggtggca
      181 gtggtaatag tgatttaaaa ggggaagcta aaggtgatgg ctcatcaaca gtagcaccaa
      241 ttgtggagaa attaaatgaa aaatgggctc aagatcactc ggatgctaaa atctcagcag
      301 gacaagctgg tacaggtgct ggtttccaaa aattcattgc aggagatatc gacttcgctg
      361 atgcttctag accaattaaa gatgaagaga agcaaaaatt acaagataag aatatcaaat
      421 acaaagaatt caaaattgcg caagatggtg taacggttgc tgtaaataaa gaaaatgatt
      481 ttgtagatga attagacaaa cagcaattaa aagcaattta ttctggaaaa gctaaaacat
      541 ggaaagatgt taatagtaaa tggccagata aaaaaataaa tgctgtatca ccaaactcaa
      601 gtcatggtac ttatgacttc tttgaaaatg aagtaatgaa taaagaagat attaaagcag
      661 aaaaaaatgc tgatacaaat gctatcgttt cttctgtaac gaaaaacaaa gagggaatcg
      721 gatactttgg atataacttc tacgtacaaa ataaagataa attaaaagaa gttaaaatca
      781 aagatgaaaa tggtaaagca acagagccta cgaaaaaaac aattcaagat aactcttatg
      841 cattaagtag accattattc atttatgtaa atgaaaaagc attgaaagat aataaagtaa
      901 tgtcagaatt tatcaaattc gtcttagaag ataaaggtaa agcagctgaa gaagctggat
      961 atgtagcagc accagagaaa acatacaaat cacaattaga tgatttaaaa gcatttattg
     1021 ataaaaatca aaaatcagac gacaagaaat ctgatgataa aaagtctgaa gacaaaaaat
     1081 aataagacgc aatttcaaat gtgtcttgaa acatgatttt gatggtgaat cattatttag
     1141 agtacaaagc ttgatttatc gagacgctga ttttgacatt cagttagtct acaagcttat
     1201 caacttaaaa tagtggttca tcattatttt acaaatctaa ttattttggg agtaatagaa
     1261 agaggtttga ttatgacttc atctactaat gttaaagctt taatcgaaaa aaataataat
     1321 aaaaaaggaa agcataatga caaaattata ccagttattt tagccgcaat ttcagcgatt
     1381 tccattttaa caacactagg tatattaatc acattgcttt tagaaaccat cacttttttc
     1441 accagaattc caataactga atttctattt tctactactt ggaatcctac cggttcagac
     1501 cctaagtttg gtatctgggc attgataata gggactttaa aaatcacagt tattgcgact
     1561 atatttgcag ttccagtcgg gcttggtgca gcaatttatt taagtgaata tgcaagtgac
     1621 cgtgcacgac gcataattaa accgatatta gaaattttag caggaatacc aacaattgtg
     1681 tttggtttct ttgcattaac ctttgttaca ccagtattaa gatctttcat accaggtctt
     1741 ggagagttta atgctataag tcccggctta gttgtcggta ttatgattgt ccctctcatc
     1801 acaagtttga gtgaggatgc aatggcatct gtaccaaata aaattcgaga aggtgcctat
     1861 ggacttggag caactaaatt agaagtagca actaaagtcg tacttcccgc agcaacatca
     1921 ggtattgtag cttcaatcgt tctcgcgatt tcaagagcaa ttggagaaac gatgattgta
     1981 tcattagcgg caggtagttc gccaacagct tcattaagtt taacaagttc gattcaaaca
     2041 atgactggat atattgttga gatagcgaca ggtgatgcaa catttggatc aaatatttat
     2101 tacagtattt atgctgtagg gttcacacta tttatcttta ccttaatcat gaatttactt
     2161 tctcagtgga tttctaagcg ttttagggag gagtattaat atggaaacga cagataataa
     2221 tagacaatca ctcgtcgatc aacaacttgt ccaaaaacat ttatcatcca gaacggttaa
     2281 aaataaagtg ttcaaactca tatttttagc atgtacatta ttaggacttg tcgtacttat
     2341 tgcgttgtta actcaaacat tgattaaagg ggtaagtcat ttaaatttac agtttttcac
     2401 taatttttct tcttcaacac catctatggc tggcgttaaa ggcgcgttaa tcggttcact
     2461 ttggttaatg ttaagtatca ttccattatc aatcatccta ggaataggta cagctatata
     2521 cttagaagaa tatgcgaaaa acaacaaatt tactcagttt gttaaaatca gtatttccaa
     2581 tttagctggt gtaccatcag ttgtatttgg gttattaggt tatactttgt tcgttggtgg
     2641 tgcagggatt gaagccttga aaatgggtaa cagtatattg gcagcagcgc taacaatgac
     2701 cttactgata ttaccaatta ttattgtttc aagtcaggaa gcaattagag ctgtacctaa
     2761 ctcagtacgc gaagcttctt acggcttagg tgctaataaa tggcaaacga taagacgtgt
     2821 tgtcttacca gcagcgttac ctggtatttt aactggattc attttgtctc tttcaagagc
     2881 actgggagaa acagcgccac ttgtgctaat cggtataccg actatattat tggcaacacc
     2941 tagaagtata ttggatcaat tttcagcatt acctatccaa atatttactt gggcgaaaat
     3001 gcctcaagaa gaattccaga atgttgcatc ggcaggcatt atcgttttac tagttatctt
     3061 aatcttaatg aatggcgttg cgattatttt acgtaacaaa tttagtaaaa aattctaatt
     3121 taaacaatca atctcattta tctattaaaa agggagtttt aaatatggcg caaacacttg
     3181 cacaaactaa acaaatatct caaagtcata cgtttgatgt ctcacaaagt catcataaaa
     3241 caccagatga tacaaactca cattctgtta tatattcaac acaaaattta gacttatggt
     3301 atggcgaaaa tcatgcatta caaaatatta atttagatat ttatgaaaac caaattactg
     3361 ccattatagg tccatctggt tgtggcaaat caacatatat taaaactttg aatcgaatgg
     3421 ttgagttagt accttctgtg aaaacagctg gtaaaatatt atatcgagat caagacattt
     3481 ttgatcaaaa atattctaaa gaacaattac gtacaaatgt gggcatggtc tttcaacaac
     3541 ctaatccatt tccaaaatca atatacgata atattactta cggtccaaag attcacggta
     3601 ttaaaaataa aaaagttctt gatgaaatcg ttgagaaatc attacgtggc gctgcaattt
     3661 gggatgaatt aaaggatagg ttgcacacaa atgcatatag tttatccggt gggcaacaac
     3721 aacgtgtttg tatcgcgcgt tgtttagcaa ttgaacctga agtcatttta atggatgaac
     3781 cgacatcagc attagatcca atctcaacat taagagtaga agagttggtt caagaactaa
     3841 aagaaaagta tacaattatt atggttacac ataatatgca acaagcagct cgtgtatcag
     3901 ataaaactgc atttttctta aatggttatg tcaatgaata tgatgatact gataaaattt
     3961 tctctaaccc atcaaacaag aaaacagaag attatatttc aggaaggttt ggttgatata
     4021 taatggcaat aattagacaa cgatatcagg agcaacttga tgatttaata aaagaattac
     4081 gtcggttagg tgcaaatgtc tatgtgagta ttgaaaatgg tataaaatca ttaagtattg
     4141 acgatagagg ctttgcacga caaacagtta aaaacgataa acatatcaat caattaaatt
     4201 atgatattaa tgagcgagtt atcatgttaa ttacaaagca acagcccatt gcgagtgatt
     4261 tgcgtatgat gatttcttca ttaaaaatcg cctccgattt agaaagaata ggagataatg
     4321 cctcgagtat tgccaatatt cgattgcgta caaagattac agatgattat gtgttaaccc
     4381 gtttaaagac aatgggtaaa ttagctatgt taatgttaaa ggacttagat caagcattta
     4441 aaaagaaaga taccgtatta ataagagaaa taattgagcg tgatgaagat atcgatgact
     4501 tatatagtca tattattaac gcaacgtatc ttattgataa cgatccattt gtcgctgcac
     4561 aagctcattt agcagcaaga catttagaac gtattggtga tcatattatt aacatcgctg
     4621 aaagtgttta tttttattta acaggtacac attacgaaca ataacttaaa gttattacta
     4681 taaaatccct tacgataaat atatatttct attattcata aaccctcaaa aaaaccaaga
     4741 ttctcacaat tagtaatgtg aaaatcttgg tttatattgt tctactataa attgtctcgc
     4801 accttagtta tttgcttgct caatttcatc tgttaatttt tcaacttcat cgactaaatc
     4861 agaaatatat tgaattgtag atttaagtgg ctgttctgta gtaatgtcta cacctgcaat
     4921 gtttgcaagt tcgacaggtg atacactacc accttttttc aatgtttcta accaagcatc
     4981 aacagctggt tggccttcat ttttaatctt ttgagaaacg acagttccga ttgttaagcc
     5041 agcagaatac gtatacgaat ataatcccat atagtaatga ggttgacgca tccatgttaa
     5101 ttcagcaccc tcagtcatgt ctactgcatc tccaaaaaat tgtttataaa catttagcat
     5161 tatttcattt aatgtcggcg catttaaaga ttcaccttga tctactttgt gatacacttc
     5221 acgttgataa gcagcttcta ataaatgggt aaccatatta tgataatatg ttctagataa
     5281 aattgagcca ataacccaac gcttaaatct tggattatca cttgtgttaa ataaataatt
     5341 ggccatcaac atttcattca ttgtagaagg ggcttcaaca aagtacattg atgcttctga
     5401 ttcaagatat ggttgatgtt tttgagctaa tgtaaaatga cctgcatgac ctaattcatg
     5461 tgctaagaca aatgcttcag ccattttacc agtccaagaa ataaacacat atgaatgtgt
     5521 aaagtatgga cttgcacaaa atgcgcctgt atctttacct ttattttgtg caaaatcaat
     5581 ccatcgctga tcgtatgctt cacgtaacat gtttgtatag tcatcaccta aaacacttaa
     5641 cgcaccgaaa atataatttt ttgagtcttc aattgaaatc tctggttcat aatcagggtc
     5701 tacagaaatc ttcaagtctt caaaacgcat gttatctaat ccgtgaatac gttgtaaaag
     5761 tttagcatat ttctgcataa ctggtgccaa gtcacgcata atcatatcga tttgacggtc
     5821 aaacatatca cgcgttactt cttgactatg taataaataa tcgatgactg attcaaatcc
     5881 acgtaaatca gcttcaattt tttcttgttg tacttgcata ttatatgtag ccgcggtagt
     5941 atgctgatat tttcgaatcc catcgctaaa cgatttgaaa cttttacgtc taaactcagg
     6001 atctttatta tcttcataat cattttcaaa cgtagcatag tcgacagggt acgttgtacc
     6061 atcatgttca aatgaatcga atgtaatatc tagcattttt gtcgtgccgt ataaatcgta
     6121 aggactgttt agcgtaggtg ataaagttgc taatacttgt tctaccgacg cagataattg
     6181 gaatggcttt tgttttatta actgtttaat atagtgttga tatggacatg attcttctaa
     6241 ttgttgaagt atttcttctg gtaattcaag tatttctgac tctacaaatg ataattggct
     6301 aacaatttta ccgtatgtag tagataattt agcgctcaat acttgtgcct cgatattact
     6361 agtatctaca cttaaacgta gttctgcata attacttaag cgatctaagg caattaaaat
     6421 attttctaat tcagctaaag cagtattaat ttgttcgatt gaatttaatg ttgttgcata
     6481 tgtatgatga aattgatttg cttgttgtac tagagcgtca atactttcat aatattgttg
     6541 atcatcttta aataagtcta ctaaatccca tgtttctgaa acaggaacat cttctcttaa
     6601 aggtaaacct tgagacatat aatcactcct ttggtataat acattcatta tacataattt
     6661 tgtctcctaa acgataatgt tacgatttcc atgtcgcaag gaacaatgat ttttctatta
     6721 gtagatagaa tattgcttga aactgactgc tattgtttaa gtttttaaaa atttataata
     6781 aaatgtatac aaggaaagta atgaaataaa aggagaatga atatgacatt attcgatatg
     6841 cctaattatt tatggatcac aacattaata atgattttat taacaatatt ctgttgctta
     6901 gttttaaata aatggtttgt atctgcagta attacatttg ttatattagg tgtgcttgca
     6961 ttttttattc caaattttca agatataaaa tatcaaccat tattaggata cgctgcattt
     7021 ttagcgatta tgagtttatt aattagtttt ctattatggt attttactag aaattggcgt
     7081 aaagaaagaa aagcgcgtaa attagaaaag caaattgaaa aatacggcta tgagggcgct
     7141 gaacttcgcc gtaaagataa aaaataatat tcgacaacta tatgcacaaa atcatcgact
     7201 tttaagttac actgcagtaa caattatttc gaaagagatg gaatacagtg ctatagttgt
     7261 ttttgttttg ac
//
LOCUS       DAXWFK010000126         7128 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.126, whole genome shotgun sequence.
ACCESSION   DAXWFK010000126 DAXWFK010000000
VERSION     DAXWFK010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7128
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.126"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(39..1400)
                     /locus_tag="ACGEXS_002030"
     CDS             complement(39..1400)
                     /locus_tag="ACGEXS_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFF8013024.1"
                     /translation="MAEKLQRELSNRHIQLIAIGGAIGTGLFLGAGQTIALTGPSILL
                     TYIIIGFMLFMFMRGLGEIIIQNTEFKSFADVTNTYIGPFAGFVTGWTYWFCWIITGM
                     AEVTAVAKYVSFWFPEIPNWISALFCVLLLMSFNLLSARLFGELEFWFSIIKIATIIG
                     LIVVGFVMILFAFKTQFGHASFTNLYEHGIFAKGASGFFMSFQMALFSFVGIEMIGVT
                     AGETKDPVKTIPKAINSVPIRILIFYVGALAVIMSIIPWQQVDPDNSPFVKLFALIGI
                     PFAAGLINFVVLTAAASSCNSGIFSNSRMLFGLSSQQQAPPNFSKTNKYGVPHVAIFA
                     SSALLLVAALLNYIFPDATKVFTYVTTISTVLFLVVWGLIIIAYINYSRKNPDLHKNA
                     TYKLLGGKYMGYLIFVFFIFVFGLLFINVDTRRAIYFIPIWFILLAFMYLRYKRIAAK
                     SNK"
     gene            1748..2869
                     /locus_tag="ACGEXS_002031"
     CDS             1748..2869
                     /locus_tag="ACGEXS_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001662816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFF8013025.1"
                     /translation="MAELQRGLEGVIAAETKISSIIESQLTYAGYDIDDLAENAQFEE
                     VIFLLWNYRLPNEEELAHLKGKLNQYMTLNPRVYTHFEEYVTDHVHPMTALRTSLSYI
                     AHFDPDAENESDENRYERAMRIQAKVASLVTAFARVRQDKEPLKPNPDLSYAANFLYM
                     LRGELPTDIEVEAFNKALILHADHELNASAFTARCAVSSLSDMYSGIVAAVGSLKGPL
                     HGGANEQVMTMLSEIGSIENVDAYLDEKFANKDKVMGFGHRVYKDGDPRAKYLREMSR
                     QITKDAGREELFEMSVKMEKRMAEEKGLIPNVDFYSASVYHCMEIPHDLFTPIFAVSR
                     SAGWIAHILEQYKDNRIMRPRAKYIGETNRKYIPLEERK"
     gene            2918..4186
                     /gene="icd"
                     /locus_tag="ACGEXS_002032"
     CDS             2918..4186
                     /gene="icd"
                     /locus_tag="ACGEXS_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFF8013026.1"
                     /translation="MTAEKITQGTEGLNVPNEPIIPFIIGDGIGPDIWKAASRVIDAA
                     VEKAYNGEKRIEWKEVLAGQKAFDTTGEWLPQETLDTIKEYLIAVKGPLTTPIGGGIR
                     SLNVALRQELDLFTCLRPVRWFKGVPSPVKRPQDVDMVIFRENTEDIYAGIEFKEGTT
                     EVKKVIDFLQNEMGATNIRFPETSGIGIKPVSKEGTERLVRAAIQYAIDNNRKSVTLV
                     HKGNIMKFTEGSFKQWGYDLALSEFGDQVFTWQQYDEIVENEGRDAANAAQEKAEKEG
                     KIIIKDSIADIFLQQILTRPAEHDVVATMNLNGDYISDALAAQVGGIGIAPGANINYE
                     TGHAIFEATHGTAPKYAGLNKVNPSSVILSSVLMLEHLGWQEAADKITDSIEDTIASK
                     VVTYDFARLMDGAEEVSTSAFADELIKNLK"
     gene            4576..5280
                     /locus_tag="ACGEXS_002033"
     CDS             4576..5280
                     /locus_tag="ACGEXS_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFF8013027.1"
                     /translation="MSQKVLVVDDEHSIVTLLKYNLETAGYEVVVAFDGDEALEKVES
                     EQPDLIILDVMLPKKDGIDVCKTVRTNKNLVPILMLTAKNDEFDRVLGLELGADDYMT
                     KPFSPREVVARVKAILRRSQFVKEVEEIDDDITIGSIRIRPDYFEVYKHNELLELTPK
                     EFELLLYLIERQGRVITREHMLNTVWNYEFAGDSRIVDVHISHLRDKLEDNPKKPQLI
                     KTVRGLGYKLERPKEQ"
     gene            5280..6941
                     /gene="pnpS"
                     /locus_tag="ACGEXS_002034"
     CDS             5280..6941
                     /gene="pnpS"
                     /locus_tag="ACGEXS_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system histidine kinase PnpS"
                     /protein_id="HFF8013028.1"
                     /translation="MKFHHRLMLLISSILIISFVTLGIIIHASITYVLSEKHEQALAT
                     DARSYIYLVQDNEIDKVKKIVKQQNIDLLITKHDKKVFSSGKLKAIPNSDSYIQKRDV
                     FIFNKKVNGYHVWIKGYNNDITEMHWTLWKYLILTCLVVLICLYFASRSFKRTLIRPI
                     QEVTYATQLLANGYYHIRVPESNVVETKALFVSTNDLARRLQKLNNEQKIQSNRLKTT
                     IENIPSAILMIDRNGKIVVANKAYYEQFNISHNIEQVGYHGYVNTEIEQLILESFKVE
                     KPIYEQLEVAINQVHAKYFDISCVPILTRSQKSLQGILVVMHDITNLKQLENLRREFV
                     ANVSHELKTPITSIKGFAETLIDGAKNDAESLDMFLNIILKESNRIESLVTDLLDLSH
                     IEQHTELDTDYMNLSDLTRRIIDNMMTQANQKNISIHTDIEKDVIVKAQESKIAQVIT
                     NLLTNAINYSYEDGDINVRVYRDDFRVIFEVQDFGIGIKLEDQQRIFERFYRVDKARS
                     RDSGGTGLGLSITKHIVEAHQGNIEVNSQVGKGSTFKVILKDYKE"
BASE COUNT         2563 a         1025 c         1333 g         2207 t
ORIGIN      
        1 gcctaagaac ttaatgtccc aagccctaaa acttgttgtt atttatttga tttagcagcg
       61 atacgtttat atcttaagta cataaatgct aaaagtataa accaaatcgg aataaaataa
      121 attgcacgtc ttgtatcaac attaataaat aataacccga acacaaaaat gaagaataca
      181 aatattaagt agcccatata tttgccacct aatagtttgt acgtagcatt tttatgtaga
      241 tctgggtttt tacgactata attgatatat gcaatgataa tcagacccca tacaactaaa
      301 aataacactg tagagatggt agtcacatac gtaaatactt ttgtcgcatc tgggaaaata
      361 tagtttagta atgctgccac aagtaataat gctgatgaag caaagattgc aacatgtgga
      421 acgccatatt tattcgtctt agaaaagttc ggaggtgctt gttgttgact tgataaaccg
      481 aaaagcatac ggctatttga gaatatacca ctgttacatg atgaagcagc agcggttaat
      541 actacaaaat taatcaagcc cgcagcaaac ggaattccga tcaatgcgaa taattttacg
      601 aatggactgt tatcaggatc aacttgctgc caagggataa tagacatgat aaccgctaac
      661 gccccaacgt aaaatattaa aattctaatg ggtacactgt taattgcttt tggaattgtt
      721 ttaactggat cttttgtttc cccagctgta acaccaatca tttcaattcc tacaaatgag
      781 aatagtgcca tttggaaaga cataaagaat ccagaagcac ctttagcgaa tatgccgtgt
      841 tcatatagat ttgtgaaact ggcatgcccg aattgagttt taaatgcaaa tagaatcatg
      901 acgaaaccaa ctactattaa accaataatt gtcgctattt taatgataga gaaccaaaat
      961 tctaattctc cgaaaagtct tgcgctaagt aggttgaatg acattaataa cagtacacaa
     1021 aatagtgcac ttatccagtt tggaatttct gggaaccaaa agctaacata ttttgccaca
     1081 gccgttactt cagccatacc tgtaataatc caacagaacc agtatgtcca tccggtaaca
     1141 aatcctgcaa aaggcccaat atatgtattg gttacatctg cgaaagattt aaattcagta
     1201 ttctgtataa tgatttctcc taaacctcgc ataaacataa ataacataaa tcctataatg
     1261 atgtatgtta atagaattga agggccggtt aatgcaatcg tttgaccagc acctaagaat
     1321 aagcctgtac caattgcacc gccaattgca attaattgta tgtggcgatt gctcagttcc
     1381 ctttgtaatt tttcagccat aatacatctc ccttaaatat agatatgttt attatgcact
     1441 tatattgaga tatatacaat tattttcggt aaaaatgtgt aaaattccat gttaatatac
     1501 tttggttttt ataatcatat ataataacca attgaaaatt taattctatt gtaaaattca
     1561 tggattattc acatcttgaa aaagctttaa tggtgctatt tgtggctatt ctgtgacatt
     1621 tacatagatt tacaaaaaaa ttgttgcaca tataatgcca gtttttatat ttcacaaacg
     1681 aaatgcgttt agctataata ttagttgaaa gccatttcat aaagaaacag taaaggggaa
     1741 atttatcatg gcagaattac aaagaggttt agaaggggtt atcgcagcgg agactaaaat
     1801 aagttcaatt attgaaagtc aattgactta tgccggctat gatattgatg atctagctga
     1861 aaatgcgcaa tttgaagaag ttattttcct attatggaac tatagattgc caaacgaaga
     1921 agagcttgct catctaaaag ggaaattaaa tcaatacatg acattaaatc ctcgtgtgta
     1981 tacacatttt gaggagtatg ttacagatca cgtgcatcca atgacagcat tacgtacgtc
     2041 attatcatat attgcacatt tcgatcctga tgctgaaaat gaatcagatg aaaatcgtta
     2101 tgaaagagca atgcgtatac aggctaaagt agcatcatta gttacagcgt ttgctcgagt
     2161 aagacaagat aaagaaccac ttaagcctaa tcctgactta agttatgcgg caaacttcct
     2221 atatatgtta cgtggggaat taccaacaga tatagaagta gaagccttca ataaagcact
     2281 tattttacac gctgatcatg agttgaacgc atctgcattt acggcacgtt gtgcggtatc
     2341 atcattgtca gatatgtact caggtattgt agcagccgta ggttctctga aagggccatt
     2401 acatggtggt gcaaacgaac aagttatgac gatgttatct gagattgggt caattgaaaa
     2461 tgttgatgct tacttagatg aaaaatttgc taataaagat aaagtaatgg gcttcggtca
     2521 tcgtgtatat aaagatggtg atcctagagc gaaatattta agagaaatga gccgtcaaat
     2581 tacgaaagac gctggtcgtg aagaattatt tgaaatgtca gtgaaaatgg aaaaacgtat
     2641 ggcagaagaa aaaggattaa ttcctaatgt tgatttttat agtgcgagtg tttatcactg
     2701 tatggaaata cctcatgact tattcacgcc aatctttgct gtaagtcgtt ctgcaggatg
     2761 gattgctcat attttagaac aatataaaga taatagaatt atgcgtccta gagcgaaata
     2821 tattggcgaa acgaatcgta agtatatccc gcttgaagaa agaaaataat caatacaaat
     2881 taaaaatgaa gatgtaaaat ttggaggtaa aataactatg actgcagaaa aaattactca
     2941 aggaactgaa ggattaaacg tacctaatga accaattatc ccatttatta tcggtgatgg
     3001 aattggaccg gatatttgga aggcagcaag ccgagttata gatgctgctg ttgagaaagc
     3061 ctataatggc gaaaaacgca ttgaatggaa agaagtgcta gctggccaaa aagcatttga
     3121 tacaactggt gaatggttac ctcaagaaac acttgataca attaaagaat atttaattgc
     3181 tgttaaagga cctttaacaa caccaattgg tggtggtatt agatcattaa atgtggcttt
     3241 acgccaagaa ttagatttat ttacttgctt aagaccggta cgttggttta aaggagtacc
     3301 atcacctgtt aaacgtccac aagatgttga tatggttatt ttccgtgaaa atactgaaga
     3361 catttatgct ggtattgaat ttaaagaagg tacaacagaa gttaaaaagg taattgactt
     3421 cttacaaaac gaaatgggtg cgacaaacat tcgattccca gaaacttcag gtattggtat
     3481 taaaccagtt tctaaagaag gaactgagcg attagttaga gcagctatac aatatgctat
     3541 cgataataac cgtaaatcag ttactttagt tcataaaggt aatattatga aatttacaga
     3601 aggctcattt aagcagtggg gttacgattt agcattatct gaatttggtg atcaagtatt
     3661 cacttggcaa caatatgacg aaattgttga aaatgaaggc agagatgctg ctaatgctgc
     3721 tcaagaaaaa gctgaaaaag aaggcaagat tatcattaaa gattctattg ctgacatttt
     3781 cttacaacaa attttaactc gtccagctga gcatgatgtt gtagcaacta tgaacttgaa
     3841 tggtgactat atttcagatg ctttagctgc acaagttggt ggtattggta ttgcgccagg
     3901 tgcaaacatt aattatgaaa caggtcatgc tatttttgaa gcaacacatg gtacagctcc
     3961 aaaatatgca ggtttaaata aagtgaatcc atcttcagta attttaagtt ctgtattaat
     4021 gttagaacat ttaggatggc aagaagcggc agataagatt acagattcaa ttgaagatac
     4081 aattgcttca aaagttgtta cttatgactt tgcccgttta atggatggtg ctgaagaagt
     4141 ttctacatca gcatttgcag atgaattgat taaaaattta aaataagcaa aataaaatta
     4201 ggtgagtcta gcaactttaa ttgctaggtt cgccttttta aatgaattga agttggcaac
     4261 aggatagaaa taataaagag tcaccaatga ttgattatgt agtgtttctt acacattaga
     4321 caaagcgaat atgtacataa gcttaggaat aaatgagtaa aactcatgca taggtagtgg
     4381 gatagaaatg ctatttttac aaaatttatt tcgttgtccc agagtaagtc atgcaaatga
     4441 tagaaggaaa tataagaagt attttattta tatgtaaagt aatgtaaaaa atgtgtaaag
     4501 ataatgtgta ggatgttatt tttataaata gttcggttat aatgtaaata taattataag
     4561 ttagggaggc ataccatgtc gcaaaaagtg ttggtagtag atgacgaaca ttcaatcgta
     4621 acattactta aatataactt agaaacagct ggttatgaag ttgttgtcgc atttgatggt
     4681 gatgaggctt tagaaaaggt agaaagtgaa cagccagatt taattatttt agatgttatg
     4741 ctacctaaaa aagatggcat tgacgtatgt aagactgtaa gaacaaataa aaatttagtc
     4801 cctatattaa tgttaacggc gaaaaatgat gaatttgatc gggtattagg tttagaatta
     4861 ggtgccgatg actatatgac taagcctttt tcacctagag aagttgttgc ccgtgtgaaa
     4921 gccattttaa gacgttctca atttgtaaaa gaagtagaag agattgacga tgatattacc
     4981 atcggatcga tacgtattag acccgattat tttgaagtat ataagcataa tgagttattg
     5041 gagctaacac cgaaagaatt tgaactcttg ctttatttga tagaaagaca aggcagagtc
     5101 attacgagag agcatatgtt aaatacggta tggaattatg aatttgcagg agactcaaga
     5161 attgttgatg tacatatcag tcatttaaga gataaattag aagataatcc taagaagcca
     5221 caacttatta aaactgtgcg tggcttaggc tataaattgg aaagacctaa agaacaatga
     5281 tgaagtttca ccaccgctta atgttattga taagcagtat attaattatc agttttgtta
     5341 cattaggcat tattattcac gcttccatca catatgtttt atcagaaaaa cacgaacaag
     5401 ctctagcaac agatgcaaga tcatatattt atttagtcca agataatgag attgataaag
     5461 ttaaaaaaat tgtaaaacag caaaatatag atttattgat aactaagcat gataaaaagg
     5521 tgtttagtag tggcaaactg aaagctatcc caaatagtga tagctatatt caaaaaaggg
     5581 acgtttttat ttttaataag aaggttaacg gttatcatgt atggattaaa ggttacaata
     5641 acgatattac agaaatgcat tggacgttgt ggaaatatct tattttgaca tgtttagttg
     5701 tactcatttg tctgtatttt gcaagccgta gttttaagcg tacattaatc cgtcccattc
     5761 aagaagttac ttatgcaaca caattgttgg caaatggtta ttatcacata cgtgtacctg
     5821 aaagtaatgt tgtagaaact aaagcgcttt ttgtgtctac caatgattta gcacgacgcc
     5881 ttcaaaagct taataatgag caaaaaattc aatccaaccg tttaaaaaca acgattgaaa
     5941 atattccaag tgctatttta atgatagatc gaaatggaaa aatcgttgtt gcaaataaag
     6001 cgtattatga acagttcaac atctcgcata atattgaaca agtaggctat cacggctatg
     6061 ttaatactga aattgagcaa ttaattttag aaagttttaa agttgaaaaa cctatttatg
     6121 aacaattgga agttgcaata aaccaagtcc atgccaagta ctttgatatt tcatgcgttc
     6181 cgattttaac taggtctcaa aaaagtttgc aagggatttt agttgtgatg catgatataa
     6241 caaatctgaa acaacttgaa aatctaagac gtgaatttgt agcaaatgtt tcacatgaat
     6301 taaaaacacc tattacttca attaaggggt ttgctgaaac gcttattgat ggtgcaaaaa
     6361 atgatgcgga atcattagac atgtttttaa acataatttt aaaagaatca aatcgaatag
     6421 aatctttagt aacagatctg ttagatttat cgcatattga gcaacataca gagttagata
     6481 cagactatat gaatttatca gatttaacgc ggcgtattat tgataatatg atgactcaag
     6541 ccaatcaaaa aaatatttcc attcatactg atattgaaaa agatgtcatt gttaaagcac
     6601 aagaaagtaa aattgctcaa gttatcacga atttgttaac gaatgcaatt aactattctt
     6661 atgaagatgg agatattaac gttcgtgtgt atcgagatga ctttcgtgtc attttcgaag
     6721 tacaagattt tggtataggt attaaattag aagaccaaca acgtattttt gaaagatttt
     6781 atagagtaga taaagcacga agtagagatt ctggtgggac aggtcttgga ttatcaatta
     6841 caaagcatat agtagaggcc caccaaggca atattgaagt gaatagtcaa gttggcaaag
     6901 gctcaacgtt caaagttatt ctaaaagatt ataaagaata aaaattaaag tggtaacagc
     6961 gcgtgtattt ttcacgaagt tgttgctgct tttttatttc gttcaaatct ctattggtat
     7021 taaattagag ttaatgaagt ggaataaatt cggttgtcaa ttttgtcatt tgtatatgtc
     7081 gatttgtaag ttataagtaa tagattgttg tgaatcaagc aattttat
//
LOCUS       DAXWFK010000127         7094 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.127, whole genome shotgun sequence.
ACCESSION   DAXWFK010000127 DAXWFK010000000
VERSION     DAXWFK010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7094
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.127"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            113..6928
                     /gene="sasA"
                     /locus_tag="ACGEXS_002035"
                     /gene_synonym="sraP"
     CDS             113..6928
                     /gene="sasA"
                     /locus_tag="ACGEXS_002035"
                     /gene_synonym="sraP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-rich repeat glycoprotein adhesin SasA"
                     /protein_id="HFF8013029.1"
                     /translation="MSKRQKAFHDSLANEKTRVRLYKSGKNWVKSGIKEIEMFKIMGL
                     PFISHSLVSQDNQSISKKMTGYGLKTTAVIGGAFTVNMLHDQQAFAASDAPLTSELNT
                     QSETVGNQNSTTIEASTSTADSTSVTKNSSSVQTSNSDTVSSEKSEKVTSTTNSTSNQ
                     QEKLTSTSESTSSKNTTSSSDTKSVASTSSTEQPINTSTNQSTASNNTSQSTTPSSVN
                     LNKTSTTSTSTAPVKLRTFSRLAMSTFASAATTTAVTANTITVNKDNLKQYMTTSGNA
                     TYDQSTGIVTLTQDAYSQKGAITLGTRIDSNKSFHFSGKVNLGNKYEGHGNGGDGIGF
                     AFSPGVLGETGLNGAAVGIGGLSNAFGFKLDTYHNTSKPNSAAKANADPSNVAGGGAF
                     GAFVTTDSYGVATTYTSSSTADNAAKLNVQPTNNTFQDFDINYNGDTKVMTVKYAGQT
                     WTRNISDWIAKSGTTNFSLSMTASTGGATNLQQVQFGTFEYTESAVTQVRYVDVTTGK
                     DIIPPKTYSGNVDQVVTIDNQQSALTAKGYNYTSVDSSYASTYNDTNKTVKMTNAGQS
                     VTYYFTDVKAPTVTVGNQTIEVGKTMNPIVLTTTDNGTGTVTNTVTGLPSGLSYDSAT
                     NSIIGTPTKIGQSTVTVVSTDQANNKSTTTFTINVVDTTAPTVTPIGDQSSEVYSPIS
                     PIKIATQDNSGNAVTNTVTGLPSGLTFDSTNNTISGTPTNIGTSTISIVSTDASGNKT
                     TTTFKYEVTRNSMSDSVSTSGSTQQSQSVSTSKADSQSASTSTSGSIVVSTSASTSKS
                     TSVSLSDSVSASKSLSTSESNSVSSSTSTSLVNSQSVSSSMSDSASKSTSLSDSISNS
                     SSTEKSESLSTSTSDSLRTSTSLSDSLSMSTSGSLSKSQSLSTSISGSSSTSASLSDS
                     TSNAISTSTSLSESASTSDSISISNSIANSQSASTSKSDSQSTSISLSTSDSKSMSTS
                     ESLSDSTSTSGSVSGSLSIAASQSVSTSTSDSMSTSEIVSDSISTSGSLSASDSKSMS
                     VSSSMSTSQSGSTSESLSDSQSTSDSDSKSLSQSTSQSGSTSTSTSTSASVRTSESQS
                     TSGSMSASQSDSMSISTSFSDSTSDSKSASTASSESISQSASTSTSGSVSTSTSLSTS
                     NSERTSTSMSDSTSLSTSESDSISESTSTSDSISEAISASESTFISLSESNSTSDSES
                     QSASAFLSESLSESTSESTSESVSSSTSESTSLSDSTSESGSTSTSLSNSTSGSTSIS
                     TSTSISESTSTFKSESVSTSLSMSTSTSLSDSTSLSTSLSDSTSDSKSDSLSTSMSTS
                     DSISTSKSDSISTSTSLSGSTSESESDSTSSSESKSDSTSMSISMSQSTSGSTSTSTS
                     TSLSDSTSTSLSLSASMNQSGVDSNSASQSASNSTSTSTSESDSQSTSSYTSQSTSQS
                     ESTSTSTSLSDSTSISKSTSQSGSVSTSASLSGSESESDSQSISTSASDSTSESASTS
                     LSDSTSTSNSGSASTSTSLSNSASASESDLSSTSLSDSTSASMQSSESDSQSTSASLS
                     DSLSTSTSNRMSTIASLSTSVSTSESGSTSESTSESDSTSTSLSDSQSTSRSTSASGS
                     ASTSTSTSDSRSTSASTSTSMRTSTSDSQSMSLSTSTSTSMSDSTSLSDSVSDSTSDS
                     TSASTSGSMSVSISLSDSTSTSTSASEVMSASISDSQSMSGSVNDSESVSESNSESDS
                     KSMSGSTSVSDSGSLSVSTSLRKSESVSESSSLSCSQSMSDSVSTSDSSSLSVSTSLR
                     SSESVSESDSLSDSKSTSGSTSTSTSGSLSTSTSLSGSESVSESTSLSDSISMSDSTS
                     TSDSDSLSGSISLSGSTSLSTSDSLSDSKSLSSSQSMSGSESTSTSVSDSQSSSTSNS
                     QFDSMSISASESDSMSTSDSSSISGSNSTSTSLSTSDSMSGSVSVSTSTSLSDSISGS
                     TSVSDSSSTSTSTSLSDSMSQSQSTSTSASGSLSTSISTSMSMSASTSSSQSTSVSTS
                     LSTSDSISDSTSISISGSQSTVESESTSDSTSISDSESLSTSDSDSTSTSTSDSTSGS
                     TSTSISESLSTSGSGSTSVSDSTSMSESNSSSVSMSQDKSDSTSISDSESVSTSTSTS
                     LSTSDSTSTSESLSTSMSGSQSISDSTSTSMSGSTSTSESNSMHPSDSMSMHHTHSTS
                     TSRLSSEATTSTSESQSTLSATSEVTKHNGTPAQSEKRLPDTGDSIKQNGLLGGVMTL
                     LVGLGLMKRKKKKDENDQDDSQA"
BASE COUNT         2574 a         1386 c         1304 g         1830 t
ORIGIN      
        1 ttacaaattt taacaaaatg agtatttttg tatcaaaaaa gagttgtatt aaattgaata
       61 gtggcatata ttataatcta atgaatgtaa agataataca aggagttatt acatgagtaa
      121 aagacagaaa gcatttcatg acagcttagc aaacgaaaaa acaagagtaa gactttataa
      181 atctggaaaa aattgggtaa aatccggaat taaagaaata gaaatgttca aaattatggg
      241 gctaccattt attagtcata gtttagtgag tcaagataat caaagcatta gtaaaaaaat
      301 gacgggatac ggactgaaaa ctacggcggt tattggtggt gcattcacgg taaatatgtt
      361 gcatgaccag caagcttttg cggcttctga tgcaccatta acttctgaat taaacacaca
      421 aagtgaaaca gtaggtaatc aaaactcaac gacaatcgaa gcatcaacat caacagccga
      481 ttccacaagt gtaacgaaaa atagtagttc ggtacaaaca tcaaatagtg acacagtctc
      541 aagtgaaaag tctgaaaagg tcacttcgac aactaatagt acaagcaatc aacaagagaa
      601 attgacatct acatcagaat caacatcctc aaagaatact acatcaagtt ctgatactaa
      661 atctgtagct tcaacttcaa gtacagaaca accaattaat acatcaacaa atcaaagtac
      721 tgcatcaaat aacacttcac aaagcacaac gccatcttcg gtcaacttaa acaaaactag
      781 cacaacgtca actagcaccg caccagtaaa acttcgaact ttcagtcgct tagctatgtc
      841 aacatttgcg tcagcagcga cgacaaccgc agtaactgct aatacaatta cagttaataa
      901 agataactta aaacaatata tgacaacgtc aggtaatgct acctatgatc aaagtaccgg
      961 tattgtgacg ttaacacagg atgcatacag ccaaaaaggt gctattacat taggaacacg
     1021 tattgactct aataagagtt ttcatttttc tggaaaagta aatttaggta acaaatatga
     1081 agggcatgga aatggtggag atggtatcgg ttttgccttt tcaccaggtg tattaggtga
     1141 aacagggtta aacggtgccg cagtaggtat tggtggctta agtaacgcat ttggcttcaa
     1201 attggatacg tatcacaata catctaaacc aaattcagct gcaaaggcga atgctgaccc
     1261 atctaatgta gctggtggag gtgcgtttgg tgcatttgta acaacagata gttatggtgt
     1321 tgcgacaacg tatacatcaa gttcaacagc tgataatgct gcgaagttaa atgttcaacc
     1381 tacaaataac acgttccaag attttgatat taactataat ggtgatacaa aggttatgac
     1441 tgtcaaatat gcaggtcaaa catggacacg taatatttca gattggattg cgaaaagtgg
     1501 tacgaccaac ttttcattat caatgacagc ctcaacaggt ggcgcgacaa atttacaaca
     1561 agtacaattt ggaacattcg aatatacaga gtctgctgtt acacaagtga gatacgttga
     1621 tgtaacaaca ggtaaagata ttattccacc aaaaacatat tcaggaaatg ttgatcaagt
     1681 cgtgacaatc gataatcagc aatctgcatt gactgctaaa ggatataact acacgtccgt
     1741 cgatagttca tatgcgtcaa cttataatga tacaaataaa actgtaaaaa tgacgaatgc
     1801 tggacaatca gtgacatatt attttactga tgtaaaagca ccaactgtaa ctgtaggcaa
     1861 tcaaaccata gaagtgggta aaacaatgaa tcctattgta ttgactacaa cggataatgg
     1921 tactgggact gtgacaaata cagttacagg attaccaagc ggattaagtt acgatagtgc
     1981 aacgaattca atcattggga caccaacaaa aattggtcaa tcaacagtga cagttgtgtc
     2041 tactgaccaa gcaaataaca aatcgacgac aacttttaca ataaatgttg tggatacgac
     2101 agcaccaaca gtgacaccaa taggagatca atcatcagaa gtgtattcac caatatcccc
     2161 gattaaaatt gctacgcaag ataacagtgg aaatgcggtg acgaatacag tgactggatt
     2221 gccatccgga ctaacatttg atagtacaaa taatactatt agtggtacac caacaaacat
     2281 tggtacaagt actatatcaa tcgtttctac agatgcgagc ggtaacaaaa cgacgacaac
     2341 ttttaaatat gaagtaacaa gaaatagcat gagtgattcc gtatcaacat caggaagtac
     2401 acaacaatct caaagtgtgt caacaagtaa agctgactca caaagtgcat caacgagtac
     2461 atcaggatcg attgtggtat ctacatcagc tagtacctcg aaatcgacaa gtgtaagcct
     2521 atctgattct gtgagtgcat ctaagtcatt aagcacatct gaaagtaata gtgtatcaag
     2581 ctcaacaagc acaagtttag tgaattcaca aagtgtatca tcaagcatgt cggattcagc
     2641 tagtaaatca acatcattaa gcgattctat ttcaaactct agcagtactg aaaaatccga
     2701 aagtctatca acaagtacat ctgattcatt gcgtacatca acatcactca gtgactcatt
     2761 aagtatgagt acatcaggaa gcttgtctaa gtcacaaagc ttatcaacga gtatatcagg
     2821 gtcgtctagt acatcagcat cattaagtga cagtacatcg aatgcaatta gtacatcaac
     2881 atcattgagc gagtcagcta gcacctcgga ctctatcagt atttcaaata gcatagccaa
     2941 ctctcaaagt gcgtcaacaa gcaaatcaga ttcacaaagt acatcaatat cattaagtac
     3001 aagtgattca aaatcgatga gtacatcaga atcattgagc gattcgacga gcacaagtgg
     3061 ttctgtttct ggatcactaa gcatagcagc atcacaaagt gtctcaacaa gtacatcaga
     3121 ctcgatgagt acttcagaga tagtaagtga ctctatcagt acaagtgggt cattatctgc
     3181 atcagacagt aaatcaatgt ccgtaagtag ttcaatgagc acgtctcagt caggtagtac
     3241 atcagaatca ttaagtgatt cacaaagtac atctgattct gatagtaagt cattatcaca
     3301 aagtactagt caatcaggtt caacaagtac atcaacgtcg acaagtgctt cagtacgtac
     3361 ttcggaatca caaagtacgt ctggttcaat gagtgcaagt caatccgatt caatgagcat
     3421 atcaacgtcg tttagtgatt caacgagtga tagcaaatca gcatcaactg catcaagtga
     3481 atcaatatca caaagtgctt ctacgagcac atctggttcg gtaagtactt cgacatcgtt
     3541 aagtacaagt aattcagaac gtacatcaac atctatgagt gattccacaa gcttaagtac
     3601 atcagagtct gattcaataa gtgaatcaac gtcaacgagc gactctataa gtgaagcaat
     3661 atctgcttca gagagcacgt ttatatcatt aagtgaatca aatagtacta gcgattcaga
     3721 atcacaaagt gcatctgcct ttttaagtga atcattaagt gaaagtacgt ctgaatcaac
     3781 atcagagtca gtgagtagtt cgacaagtga gagtacgtca ttatcagaca gtacatcaga
     3841 atctggtagc acatcaacat cattaagtaa ttcaacaagt ggtagtacgt ccatttcaac
     3901 atcgacaagt atcagtgaat caacgtcaac gtttaagagc gagagtgttt caacatcact
     3961 gagtatgtca acgagtacaa gtttgtctga ctctacaagt ttgtcaacat cattaagtga
     4021 ttccacaagt gatagtaagt ctgattcatt aagtacatca atgtcgacaa gtgattcaat
     4081 cagtacaagt aaatctgatt ccattagtac atccacatca ttaagtggtt ctacaagtga
     4141 aagtgaatcc gactcaacat catcaagtga aagtaaatcc gattcaacat caatgagcat
     4201 aagtatgtct caatcaacat caggaagtac aagtacgtca acgagtacaa gtttgtctga
     4261 ctcaacgagt acatcattgt cactaagtgc ctcaatgaat caaagcggag tagactcaaa
     4321 ctcagcaagc caaagtgcct caaactcaac aagtacaagc acgagcgaat ccgattcaca
     4381 aagcacatca tcatatacaa gtcagtcaac aagccaaagt gaatccacat cgacatcaac
     4441 gtcactaagc gattcaacaa gtatatctaa aagtacgagt caatcaggtt cggtaagcac
     4501 atcagcgtca ttaagtggtt cagagagtga atctgattca caaagtatct caacaagtgc
     4561 aagtgattca acatcagaaa gtgcgtcaac atcactcagt gactcaacaa gtacaagtaa
     4621 ctcaggatca gcaagtacgt caacatcgct cagtaactca gcaagcgcaa gtgaatccga
     4681 tttgtcgtca acatctttaa gtgattcaac atctgcgtca atgcaaagca gtgaatccga
     4741 ttcacaaagc acatcagcat cattaagtga ttcgctaagt acatcaactt caaaccgcat
     4801 gtcgaccatt gcaagtttat ctacatcggt aagtacatca gagtctggct caacatcaga
     4861 aagtacaagt gaatccgatt caacatcaac atcattaagc gattcacaaa gcacatcaag
     4921 aagtacaagt gcatcaggat cagcaagtac atcaacatca acaagtgact ctcgtagtac
     4981 atcagcttca actagtactt cgatgcgtac aagtactagt gattcacaaa gtatgtcgct
     5041 ttcgacaagt acatcaacaa gtatgagtga ttcaacgtca ttatctgata gtgttagtga
     5101 ttcaacatca gactcaacaa gtgcgagtac atctggttcg atgagtgtgt ctatatcgtt
     5161 aagtgattcg acaagtacat caacatcggc tagtgaagta atgagcgcaa gcatatctga
     5221 ttcacaaagt atgtcaggat ctgtaaatga ttcagaaagt gtaagtgaat ctaattctga
     5281 aagtgactct aaatcgatga gtggctcaac aagtgtcagt gattctggct cattgagcgt
     5341 ctcaacgtca ttaagaaaat cagaaagtgt aagcgagtca agttcattga gttgctcaca
     5401 atcgatgagc gattcagtaa gcacaagcga ttcgtcatca ttaagtgtat cgacgtcact
     5461 aagaagttca gaaagcgtga gtgaatctga ttcattaagt gattcaaaat caacaagtgg
     5521 ttcgacttca acaagtacat ctggttcatt gagtacctca acatcattaa gtggttcaga
     5581 aagcgtaagc gagtctacct cgctaagtga ttcaatatca atgagtgatt ctactagtac
     5641 aagtgactcc gactcattaa gtggatcaat atctttaagt ggttccacaa gtcttagcac
     5701 ttcggattca ttaagtgatt caaaatcatt gagtagctcg caaagtatga gtggatcaga
     5761 atcaacgtca acaagtgtga gcgattcgca gtcaagctca acaagtaata gtcaatttga
     5821 ctctatgagc atcagtgcat cagaaagcga ctcaatgtct acaagtgatt cgtctagcat
     5881 cagtggatca aattcaacga gtacatcact ttcaacatct gactcaatga gcggaagcgt
     5941 atcagtttca acatcgacaa gtttaagtga ctcaatatca ggttcaacaa gtgtaagtga
     6001 ctcgagctca acaagcacat ctacatcatt aagtgattca atgtcacaaa gccagtcaac
     6061 aagtacaagt gcatctggtt ccttaagtac atcgatatca acatcaatgt caatgagtgc
     6121 tagtacatcg tcatcacaaa gcacatcggt gtcgacatca ttatcaacat cagacagtat
     6181 cagtgattct acttcaataa gtatcagtgg ttcacaaagt acagtagaat cagaatctac
     6241 aagtgattca acttctatca gtgactcaga atcattgagt acatcagatt cagactcgac
     6301 atcgacaagt acatcggact caacaagtgg ttcaacttca acaagcatat ctgaatcatt
     6361 aagtacgtct ggttcaggtt caacgagcgt atctgactca acatcaatga gtgaatctaa
     6421 ttcatcgagt gtttcaatgt cacaagacaa atccgactca acatcaatta gtgactcaga
     6481 atcagtgtca acaagcacat caacgtcatt gagcacatcc gattcgacaa gcacatccga
     6541 atcactgagt acatctatgt ctggttcaca aagcatttct gactcaacat caacaagtat
     6601 gtccggctca acaagtacat ctgaatctaa ctcaatgcat ccgtcagact caatgagtat
     6661 gcatcatact cacagcacga gcacatctcg cttatcaagt gaagcaacaa cgagcacgag
     6721 tgaatctcag tctacattaa gtgcaacatc tgaagtgact aaacataatg gcacaccagc
     6781 acaaagtgaa aaaagattgc cagatacagg tgactcaata aaacaaaatg gattactagg
     6841 tggcgttatg acattattag ttggtttagg tttaatgaag agaaagaaaa agaaagatga
     6901 aaatgatcaa gatgattctc aagcataaat tgaatttata gctggctaaa attaaaaaga
     6961 ctgaggcacc aatcactaga aaaatcgata taggatttta ataattagtg atgaaccctt
     7021 agtcttttta atgtttttga tataaaagag gtgcaacatg ttaaaacttt tacaacaata
     7081 cgaatataaa atta
//
LOCUS       DAXWFK010000128         7061 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.128, whole genome shotgun sequence.
ACCESSION   DAXWFK010000128 DAXWFK010000000
VERSION     DAXWFK010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7061
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.128"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            17..883
                     /locus_tag="ACGEXS_002036"
     CDS             17..883
                     /locus_tag="ACGEXS_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HFF8013030.1"
                     /translation="MDVIKQIQQAIVYIEDRLLEPFNLQELSDYVGLSPYHLDQSFKM
                     IVGLSPEAYARARKMTLAANDVINGATRLVDIAKKYHYANSNDFANDFSDFHGVSPIQ
                     ASTKKDELQIQERLYIKLSTTERAPYPYRLEETDDISLVGYARFIDTKYLSHPFNVPD
                     FLEDLLIDGKIKELRRYNDVSPFELFVISCPLENGLEIFVGVPSERYPAHLESRFLPG
                     KHCAKFNLQGEIDYATNEAWYYIESSLQLTLPYERNDLYVEVYPLDISFNDPFTKIQL
                     WIPVKQSPYDED"
     gene            complement(966..2615)
                     /locus_tag="ACGEXS_002037"
     CDS             complement(966..2615)
                     /locus_tag="ACGEXS_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFF8013031.1"
                     /translation="MNKQWWKEAVAYQVYPRSFNDSNHDGIGDLPGMIDKLDYLKDFG
                     IDVIWLSPMFKSPNDDNGYDISDYQEIMDEFGTMEDFDRLLKGVHDRGMKLILDLVVN
                     HTSDEHPWFIESKSSKDNPKRDWYIWQDPKPDGSEPNNWESIFNGSTWEYDANTEQYY
                     FHLFSKKQPDLNWGNPEVRDAVFEMMNWWFDKGIDGFRVDAITHIKKTFEAGDLPVPE
                     GKTYAPAFDVDMNQPGIQTWLQEMKDRSLSKYDIMTVGEANGVSPDDADDWVGEENGK
                     FNMIFQFEHLGLWNSGDSHFDVNSYKSVLNRWQKQLENKGWNALFIENHDQPRRVSTW
                     GDDDKYWYESATSHAAVYFLQQGTPFIYQGQEIGMTNYPFESIETFNDVAVKNDYQIV
                     KAQGGDVDALLAKYKDENRDNSRTPMQWDDTLNGGFTNGEPWFPVNPNYKTINVAQQL
                     EDEHSVLQFYKDLIQLRKSNDVYVYGQFDLVDAENSQVFAYTRTLNEKQVLIVGNLTN
                     HEAELTVPFDLSHGEVKLFNYDAKVNLKQLRPYEACVIELN"
     gene            complement(2631..3650)
                     /locus_tag="ACGEXS_002038"
     CDS             complement(2631..3650)
                     /locus_tag="ACGEXS_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8013032.1"
                     /translation="MVTIKDVALKAGVSPSTVSRVIKGNQRISEATISKVKKVMEELN
                     YFPNTAARTLITNQTYKIGLVLKGSEEPIRLNPFYINVLLGISETCNQHGYGTQTTVS
                     NNMNDLMDEVYKMIKQRMVDAFILLYSKENDPIKQMLIDESMPFIVIGKPTSDIDHQF
                     THIDNDNILASENLTRHVIEQGVDELIFITEKGNFEVSKDRIQGFETIASQNKINYQI
                     IETSNEREVIFNYMQNLHTRLKDPNIKQAIISLDAMLHLAILSVLYELNIEIPKDVMT
                     ATFNDSYLTEIASPPQTCIDIKPRMLGQQAGAAILNILKNKAQDVIELVIIDTELKIR
                     KSTQR"
     gene            complement(3651..3905)
                     /locus_tag="ACGEXS_002039"
     CDS             complement(3651..3905)
                     /locus_tag="ACGEXS_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013033.1"
                     /translation="MVNVYLIFKMAKVTILQLVTKQSILIFIQTFILIFGLNFSMKVL
                     FLSFYAIVCTNLLTFLESVTKLVLYSGWKRFPMQKLTGGD"
     gene            complement(3844..4215)
                     /locus_tag="ACGEXS_002040"
     CDS             complement(3844..4215)
                     /locus_tag="ACGEXS_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFF8013034.1"
                     /translation="MEKLNQVMLYVDDVEKAKAFWTETLEFVVVSETPLAEDYVAVEV
                     SPTKDAETSLTIMAKEFIEKHSPEVNLGTPSLMFKEKNFDALYSKLNDLGLTGHDIVE
                     MNGQRVFNFQDGQGNYFAVSD"
     gene            complement(4434..5840)
                     /gene="gndA"
                     /locus_tag="ACGEXS_002041"
     CDS             complement(4434..5840)
                     /gene="gndA"
                     /locus_tag="ACGEXS_002041"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFF8013035.1"
                     /translation="MTQQIGVIGLAVMGKNLAWNIESRGYSVSVFNRSSEKTDLMVEE
                     SKGKNIHPTYSLEEFVNSLEKPRKILLMVQAGKATDATIDSLLPLLDDGDILIDGGNT
                     NYQDTIRRNKALAQSAINFIGMGVSGGEIGALTGPSLMPGGQEEAYNKVADILDAIAA
                     KAKDGASCVTYIGPNGAGHYVKMVHNGIEYADMQLIAESYAMMKELLGMSHEDIAQTF
                     KDWNAGELESYLIEITGDIFMKLDENKEALVEKILDTAGQKGTGKWTSINALELGIPL
                     TIITESVFARFISSIKEERVNASKELNGPKASFDGDKKDFLEKIRKALYMSKICSYAQ
                     GFAQMRKASEDNEWNLKLGDLAMIWREGCIIRAQFLQKIKDAYDNNPGLQNLLLDPYF
                     KNIVTEYQDALRDVVATGVQNGVPTPGFSSSINYYDSYRAADLPANLIQAQRDYFGAH
                     TYERKDKEGVFHTQWIEE"
     gene            complement(5908..7041)
                     /locus_tag="ACGEXS_002042"
     CDS             complement(5908..7041)
                     /locus_tag="ACGEXS_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripeptidase T"
                     /protein_id="HFF8013036.1"
                     /translation="MINEQRLLNTFFELVQIDSETGNESTIQPILKEKFIALGLDVKE
                     DEAAKHPKLGANNLVCTMNSTIEEGEVPKLYLTSHMDTVVPAINVKPIVKDDGYIYSD
                     GTTILGADDKAGLAAMLEVLQVIKEQQIPHGQIQFVITVGEESGLIGAKELNSELLDA
                     DFGYAIDASADVGTTVVGAPTQMLISAKIFGKTAHASTPKEGVSAINIAAKAISRMKL
                     GQVDEITTANIGKFHGGSATNIVADEVILEAEARSHDPERIKTQVKHMTDVFETTASE
                     LGGKAVVTVEQSYPGFKINDNEAVVKIAQESARNLGLSANTIISGGGSDGSIINTFGI
                     PSVILGVGYEKIHTTNERMPIKSLNLLASQVLEIIKIVARHSK"
BASE COUNT         2179 a         1378 c         1001 g         2503 t
ORIGIN      
        1 aattggagtg aaagttttgg acgttatcaa gcaaatacaa caggcaattg tttatattga
       61 agatcgttta ttagagcctt tcaatttgca agaattaagt gattacgttg gtctttcgcc
      121 ataccatctt gatcaatcat ttaaaatgat tgtcggctta tctccagaag cttatgcacg
      181 cgcgcgtaaa atgacactcg ctgcaaatga tgtgattaat ggtgctacac gacttgtaga
      241 tatcgctaaa aaatatcact atgcaaattc aaatgatttt gcaaatgatt ttagtgattt
      301 tcacggcgta tcacctattc aagcctctac taaaaaagat gaattacaaa ttcaagagcg
      361 attatatatc aaattatcaa ctactgagag agcaccttat ccatacagat tagaagagac
      421 agatgatatt tcattggttg gatatgcacg atttatagac actaagtatt tgtcacatcc
      481 ttttaatgtt ccggattttt tagaagactt gctcattgat ggtaaaatta aagagttacg
      541 acgatataat gacgttagtc catttgaact atttgttatt agttgtcctc ttgaaaatgg
      601 tttagaaata tttgtaggtg taccaagtga acgttatcct gcacacttag aaagtcgatt
      661 tttacctggc aaacattgtg cgaaattcaa tttacaaggt gaaattgatt atgcaactaa
      721 tgaagcttgg tactatattg aatcaagttt gcagttaaca ttgccatatg aacgaaatga
      781 tttatatgtt gaagtgtacc ctctcgatat ttcatttaat gacccattca ctaaaattca
      841 gctttggatt cctgttaaac agagtcctta tgacgaagat taaataataa aaaacaaaga
      901 agccccctaa tatatctata ggtctacaaa tggccttaga ttctattagg gggcatatta
      961 atatgttaat ttagttcgat aacacatgct tcatatggac gtaactgttt taaattaact
     1021 ttggcatcat aattaaatag ctttacttct ccatggctta aatcaaatgg tacagttaat
     1081 tctgcttcgt ggttagtaag attacctaca ataagaactt gcttttcatt taatgttctc
     1141 gtgtacgcaa aaacttgtga attttcagca tctactaaat caaattgacc atatacgtat
     1201 acatcattag actttcttaa ttgaattaaa tctttataaa attgtaatac tgaatgctca
     1261 tcttctaatt gttgtgcaac attgatagtt ttataattcg gattcactgg gaaccacggt
     1321 tcaccatttg taaatcctcc atttaacgta tcatcccatt gcattggtgt gcgagaatta
     1381 tctcggttct catctttata tttcgcaagt aaagcgtcta catctccacc ttgagctttc
     1441 actatttgat agtcattttt aacagcaaca tcgttaaacg tttcaatact ttcaaatgga
     1501 taattcgtca taccaatttc ttgaccttga taaatgaatg gcgtaccttg ttgcaagaaa
     1561 taaacagctg catgacttgt tgctgattca taccaatact tgtcatcgtc accccacgtc
     1621 gatacacgtc gtggttggtc gtgattttca ataaacaacg cattccaacc tttattttca
     1681 agttgttttt gccatctatt taatacagat ttatacgaat ttacatcaaa gtgagaatca
     1741 ccactattcc acagtcccaa atgttcaaat tggaatatca tattaaattt accattttct
     1801 tccccgaccc agtcatcagc atcatcaggg cttacaccat tcgcttcacc aacagtcata
     1861 atgtcatact tacttaatga gcgatctttc atctcttgta accaagtttg tatacctggc
     1921 tgattcatat ctacatcaaa tgctggggca tatgttttac cctcaggtac aggtaagtca
     1981 cccgcttcaa acgtcttctt aatatgcgta attgcatcta ctctaaatcc atcaatgcct
     2041 ttatcaaacc accagttcat catttcaaat acagcatctc taacttccgg attaccccaa
     2101 ttcaaatcag gttgtttttt actgaataaa tggaaataat attgctcagt attagcatca
     2161 tattcccatg tagatccatt aaatatactt tcccagttgt taggttcaga gccatctggc
     2221 tttggatctt gccaaatgta ccaatcacgt ttgggattgt ctttactaga tttggattct
     2281 ataaaccaag gatgttcatc agatgtatga tttacaacta aatctaaaat aagcttcatg
     2341 cctctatcat gaacaccttt taataaacga tcaaagtctt ccatcgttcc aaattcatcc
     2401 ataatctctt ggtagtcact aatatcataa ccattgtcat cattaggtga tttaaacatt
     2461 ggactgagcc aaatgacatc gataccgaaa tcttttaagt agtccaattt atcaatcatt
     2521 ccaggtaaat ccccaatacc atcgtgatta ctatcattaa aacttcttgg atatacttga
     2581 tatgctactg cttctttcca ccattgctta ttcattttaa aactcctttg ctatcgctgt
     2641 gttgattttc ttatttttaa ttctgtatct ataatgacga gttcaataac atcctgtgct
     2701 ttgtttttca atatatttaa aattgctgca ccagcctgtt gacctaacat tcgaggcttg
     2761 atgtcaatac aggtttgtgg tggtgacgca atttcggtta aataagaatc attgaacgtt
     2821 gctgtcatta catctttcgg aatttcaata ttaagttcat ataggacact taaaatcgct
     2881 aaatgtaaca tagcatctaa cgaaatgatt gcctgtttaa tatttgggtc cttcaaacgc
     2941 gtatgtagat tttgcatgta attaaaaata acttctcttt cattactagt ctcaataatt
     3001 tgataattaa ttttattttg agaagctatc gtttcaaatc cttgaattct atcttttgaa
     3061 acttcaaaat ttcctttttc tgtaataaat attaattcat ctacaccttg ttcaataaca
     3121 tgtcgtgtca aattttcaga agctaatata ttatcattat ctatatgtgt aaattgatga
     3181 tctatatccg atgtaggctt accaatcaca ataaatggca tgctttcatc aattaacatt
     3241 tgtttaatcg gatcattttc ttttgaatag agcagtataa acgcatcaac cattcgttgt
     3301 ttaatcattt tataaacttc atccattaaa tcattcatat tatttgagac tgtcgtttgt
     3361 gtaccatagc catgctggtt acacgtttca gaaattccta gcaatacatt gatgtagaat
     3421 ggattcagtc gaataggctc ctcagaccct tttaacacta aaccaatttt atatgtttgg
     3481 tttgtaatta aagttctagc agcggtatta ggaaaataat tcaattcttc cataactttc
     3541 ttcacttttg aaattgtcgc ttcgctaata cgttgatttc cttttataac tcttgaaact
     3601 gtcgaaggag aaacaccggc ttttagtgca acatctttaa tcgtaaccat ttaatcacct
     3661 cctgttaatt tctgcattgg aaaacgcttc caaccactgt ataataccag tttagtcaca
     3721 ctttctaaaa aagtcaaaag atttgtgcaa acgattgcat aaaacgataa aaataaaacc
     3781 ttcatactga aattcaatcc gaaaatcaat ataaaggttt gtataaatat taaaatcgat
     3841 tgtttagtca ctaactgcaa aatagttacc ttggccatct tgaaaattaa atacacgttg
     3901 accattcatt tctactatat catgcccagt taaacctaaa tcatttaatt ttgagtataa
     3961 tgcatcaaag tttttctctt taaacattaa agatggtgtt cctaggttca cttccgggct
     4021 atgcttttca ataaattctt ttgccataat cgtcaatgac gtttcagcat ctttggtagg
     4081 tgatacttca actgcaacat agtcctcagc taacggtgtt tcacttacaa caacaaattc
     4141 taaagtttct gtccaaaatg ctttcgcttt ttcgacatca tcaacatata acataacttg
     4201 atttaacttt tccataaaat agtacctcta tttctctata gtacatgcta tcataacaca
     4261 gtaaatattt tattacttca caaaatgctt aaaaatatgg cgggatgctt ttaaggtcaa
     4321 ggataatact tgtgtaattt tttataggtt gtagctactc tatcacactc tcttttatat
     4381 ttatcaaaag atataaaaaa ggatagtatc tttcaactat cctttaatca atattattct
     4441 tcaatccatt gtgtatggaa tacgccttct ttatcttttc tttcgtacgt atgagcaccg
     4501 aagtagtcac gttgtgcttg aattaagttt gcaggtaaat cagcagcacg gtaactatca
     4561 tagtaattaa tacttgatga gaaaccaggt gttggtacac cattttgaac accagttgcg
     4621 acaacatcac gtaacgcatc ttgatattca gtaacgatgt ttttaaagta aggatctagc
     4681 aataagtttt gtaatcctgg attattatcg taagcatctt tgatcttttg taagaattgt
     4741 gcacggataa tgcaaccttc tctccaaatc atagctaaat caccaagttt taaattccat
     4801 tcattatctt cacttgcttt acgcatttgc gcgaaacctt gtgcataaga acaaatttta
     4861 ctcatatata atgctttacg aattttttct aaaaagtctt tcttgtcacc atcaaatgat
     4921 gcttttggac catttaattc tttagaagca tttacgcgct cttctttgat tgaagagata
     4981 aaacgtgcaa atacagattc agtaatgatt gttaatggaa tacctaattc taatgcgtta
     5041 attgaagtcc attttcctgt acctttttga cctgcagtat caagaatttt ttcaactaat
     5101 gcttctttat tttcatctaa tttcatgaaa atatcaccag tgatttcaat taaataactt
     5161 tctaattcac cagcattcca gtctttgaac gtttgagcaa tgtcttcatg agacatgcct
     5221 aataattctt tcatcatagc ataactttct gcaattaatt gcatgtcagc atattcgata
     5281 ccattgtgta ccattttcac atagtgtcca gcaccattag gtccaatata agtaacacat
     5341 gaagcaccgt cttttgcctt tgcagcaatt gcatcaagaa tatctgcaac tttgttataa
     5401 gcttcttctt gtccacccgg cattaatgac ggaccagtta acgctccaat ttcaccacca
     5461 gaaacgccca taccaataaa gttgattgca ctttgtgcta atgctttatt acgtctgata
     5521 gtatcttgat agtttgtatt accaccatca attaaaatat ctccatcatc taataaaggt
     5581 aacaaactat caatcgttgc gtccgtagct ttacctgctt gaaccattaa taaaatttta
     5641 cgtggttttt ctaaagaatt aacaaattct tccaatgaat acgttggatg aatatttttc
     5701 ccttttgatt cttcaaccat taaatcagtt ttttcacttg agcggttaaa tacagataca
     5761 ctatatccgc gtgattcaat attccaagct aggtttttac ccataacggc taaaccaata
     5821 actccaattt gttgtgtcat attacttacc tcacttgttg atttttcatt agtattgtat
     5881 cacaaaatag acatacacta cactaaatca tttcgaatgt cgcgcaacta ttttgattat
     5941 ttctaacact tgacttgcaa gcaagttcaa tgatttaatc ggcattctct catttgttgt
     6001 atggattttt tcataaccca ctcctaaaat gactgaagga ataccaaatg tattaataat
     6061 actgccgtct gaaccgccac cagaaataat tgtatttgca gataatccta aattacgagc
     6121 actttcttgt gcaattttaa caaccgcttc attatcatta attttaaatc ctggataact
     6181 ttgctccact gtaactactg ctttcccacc taattctgat gcagtagttt caaacacatc
     6241 agtcatatgt ttgacttgtg tttttattct ttctggatcg tgagaacgtg cctctgcttc
     6301 taaaatgact tcatctgcaa caatattcgt agctgaaccg ccatgaaact taccaatatt
     6361 ggcagtagtt atttcatcaa cttgtcctaa tttcattcga ctaattgctt tcgccgcaat
     6421 attaatagca ctaacaccct cttttggcgt acttgcatga gccgttttgc caaaaatttt
     6481 agctgaaatt aacatttgcg tcggtgcacc tacaaccgta gtaccgacat cagcacttgc
     6541 atcaatagca taaccaaagt ccgcgtccaa caactctgaa tttaattctt tagcaccaat
     6601 taaacctgat tcttctccaa cagtaatcac aaattgaatt tgtccatgtg ggatttgttg
     6661 ttcctttatc acttgcaaaa cttcaagcat cgctgctaat cctgctttat catctgcacc
     6721 tagaatagtc gtaccatcag agtatatgta gccgtcatct tttacaattg gctttacatt
     6781 aattgcgggt acaacagtat ccatatggct cgtcaaatat aatttaggta cttcgccttc
     6841 ttcgatagta ctattcattg tacacactag attattggca cctaatttag gatgtttagc
     6901 cgcttcatct tctttaacat ctaaccctaa tgctatgaat ttttctttta aaataggttg
     6961 gattgttgat tcattccctg tctcagaatc gatttgtaca agttcaaaaa acgtattaag
     7021 taatctttgc tcattaatca taatggaacc ccttttatca a
//
LOCUS       DAXWFK010000129         7044 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.129, whole genome shotgun sequence.
ACCESSION   DAXWFK010000129 DAXWFK010000000
VERSION     DAXWFK010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7044
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.129"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            131..937
                     /locus_tag="ACGEXS_002043"
     CDS             131..937
                     /locus_tag="ACGEXS_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFF8013037.1"
                     /translation="MKIGTIADLHIDRHNKKTSEDYLEALVEIVKYKKLDILLIAEDI
                     SNHYQLTHQFITQLTKQLDIPVKFVPGNHDLWEVESMTTQDIWNNYKSMSQCLVGKPF
                     IVNEEWAIIGHTGWYDYSFAAQRFSLDELQKGKHYGATWQDKERISWGISDQNLSKIA
                     AEQVKKDILEVGNRRVILVTHVVTHPDFIVPMPHRIFDFYNAFIGTSDFNPLYAMFDI
                     PYSIMGHVHFRKSVIDDGRCYLCPCLGYPREWRSEDIYQEINETIQIIEI"
     gene            complement(1000..1656)
                     /locus_tag="ACGEXS_002044"
     CDS             complement(1000..1656)
                     /locus_tag="ACGEXS_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFF8013038.1"
                     /translation="MNRKILPVKPISQLFPIPMVMGCEGVSAAMMLQYNNQHIPATEI
                     MRHWPTHPNNPHKGYVGHHLFIKLGNYHQTIFPEAYVPFLQKYNPNIVDGTGKSLEDL
                     KGIIDQGQPVLIYHTNLGSKPLLRVFRFDNKPAKQVSNIHVTLLIGYDDYYYYYIDPL
                     WSHIRRGLVLPAIIPNRKQIIKIRKEKMEYSFNSPGRKCIYVQPHSYTIENQQQNKHT
                     "
     gene            1769..2242
                     /locus_tag="ACGEXS_002045"
     CDS             1769..2242
                     /locus_tag="ACGEXS_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFF8013039.1"
                     /translation="MEYQLQQLASLTLVGIKETYENGRQAQQHIAGFWQRCYQEGVIA
                     DLQLKNNGDLAGILGLCIPELDGKMSYMIAVTGDNSADIEKYDVITLASSKYMVFEAQ
                     GAVPKAVQQKMEEVHHYIHQYQADTVKSAPFFELYQDGDTTSEKYITEIWMPVKG"
     gene            complement(2365..3576)
                     /locus_tag="ACGEXS_002046"
     CDS             complement(2365..3576)
                     /locus_tag="ACGEXS_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HFF8013040.1"
                     /translation="MQSTKTKTKHFSFLLLITLGVMTAFGPLTIDMYVPSLPKVQGDF
                     GSTTSEIQLTLSFTMIGLALGQFIFGPLSDAFGRKRIAVSILIIFILVSGLSMFVDQL
                     PLFLTLRFIQGLTGGGVIVIAKASAGDKFSGNALAKFLASLMVVNGIITILAPLAGGL
                     ALSVATWRSIFTILTIVALIILIGVASQLPKTSKDELKQVNFSSVIKDFGSLLKKPAF
                     IIPMLLQGLTYVMLFSYSSASPFITQKLYNMTPQQFSIMFAVNGVGLIIVSQVVALLV
                     EKLHRHILLIILTIIQVVGVALIILTLTFHLPLWVLLIAFFLNVCPVTSIGPLGFTMA
                     MEERTGGSGNASSLLGLFQFILGGAVAPLVGLKGEFNTSPYMIIIFITAILLVSLQII
                     YFKMIKKQHVA"
     gene            complement(3758..4417)
                     /locus_tag="ACGEXS_002047"
     CDS             complement(3758..4417)
                     /locus_tag="ACGEXS_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013041.1"
                     /translation="MKRTDKYRDSYQYDNQNQNHRRQSEDASYRQQYAKGDPEEHPER
                     YYNGRDYRREQILEEENEKSRRSKKWLYIIIAILLIIVAIFVTRALLNNDSDKVSNDP
                     KVSQNYKKQVENQDGQINQQVDNAKENIKNNQKTDDIIKNLQNQIDNLKQQEQNKADS
                     KLTQFYQDQINKLTEANNALKNNASQGKIESMLNDINTKFDSIKSKLESLFKDDNGGA
                     N"
     gene            complement(4477..5619)
                     /locus_tag="ACGEXS_002048"
     CDS             complement(4477..5619)
                     /locus_tag="ACGEXS_002048"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFF8013042.1"
                     /translation="MQLQKIVIAPDSFKESMTAQQVGNIIKQAFTNVYGNTLHYDIIP
                     MADGGEGTTDALMHATGATKYTVIVNDPLMRPIEACYARADEQQIAIIEMAAASGLDL
                     LEKEERNPLYTSSYGTGELIKDALNHGAKTIILGIGGSATNDGGTGMLSALGVKFTDV
                     NGDLLQMNGANLAHIAQIDITNLDSRLKEVTFKVACDVSNPLLGENGATYIYGPQKGA
                     DAKMIPKLDFAMSHYHDKIKMCTGKSVNQIPGSGAAGGMGAALLAFCETTLTKGIDVV
                     FDITDFHQRIKDADLVITGEGRMDYQTIFGKTPVGVALAAKQYHIPVIAICGSLGENY
                     QHVYDFGIDSAYSIISSPSTLEDVLQNSEQNLLNTATDIARILKLQ"
     gene            5887..6273
                     /gene="gtxA"
                     /locus_tag="ACGEXS_002049"
     CDS             5887..6273
                     /gene="gtxA"
                     /locus_tag="ACGEXS_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase GtxA"
                     /protein_id="HFF8013043.1"
                     /translation="MKLTQTHAEILKFIIVGGINTLNYYVVYLLLLKLLHIEYMISHI
                     TGFLVAFVISYYLNCYFVYRVKPTWRKFISFPITQIVNVSLQTVLLYVFVSWLNLPAE
                     IAPFAGLIITIPITFILSKWILKDSN"
     gene            6406..6513
                     /locus_tag="ACGEXS_002050"
     CDS             6406..6513
                     /locus_tag="ACGEXS_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFF8013044.1"
                     /translation="MFNLLINIMTSAISGCLVAFFAHWLRTRNNKKGDK"
BASE COUNT         2332 a         1092 c         1300 g         2320 t
ORIGIN      
        1 ttttttgaaa agatataagt ttttatgcaa aaatataaaa taatcatcta aatgaagcag
       61 ttaactatat atattagata agctatggca taataaataa cattttaatt tgagtaaaga
      121 aggtaagggt atgaagatag ggacaatcgc cgatttacat attgatcgtc ataataaaaa
      181 aacatccgaa gattacttag aggcattggt tgaaatagtt aagtataaga aattggatat
      241 attgttgatt gcggaggata tctcaaatca ttatcaattg acgcaccaat ttataacgca
      301 attgacaaaa caattagata tacctgtgaa atttgtacct ggaaaccatg atttatggga
      361 agttgaaagt atgactacgc aagacatttg gaataattat aagagtatgt cacagtgctt
      421 ggtaggaaaa ccatttatag taaatgaaga atgggcaatc ataggacata ctggctggta
      481 tgattatagc tttgcagcac aacgattttc attagatgag ttacaaaaag gaaaacatta
      541 tggtgcgact tggcaagata aagaacgaat atcttggggc atatcagatc aaaatttatc
      601 taaaatagcg gctgaacaag tgaagaaaga tatattagaa gtaggaaata gacgagtgat
      661 tttagtcaca catgttgtga cgcaccctga tttcattgtt cctatgccgc atcgtatatt
      721 cgatttttat aatgcattta ttgggacaag tgatttcaat cctttgtatg cgatgttcga
      781 tataccatat agtattatgg gccatgttca ttttcgtaaa agtgtgatag atgatggcag
      841 atgttatctc tgtccgtgtc taggctatcc aagagaatgg cgttcagaag atatttacca
      901 ggaaataaat gagacgatac aaataataga aatttaaaat gcgcaaacct gacccagttt
      961 gcgcatttta tgttttacac acgcgagtaa tgtgtttact tacgtgtgtt tattttgttg
     1021 ctgattttca attgtatatg aatgtggttg cacataaatg cactttcttc ctggtgaatt
     1081 aaagctgtat tccattttct ctttacgaat tttaataatt tgtttgcgat ttggaatgat
     1141 ggcaggtaaa actaggccac gacgaatatg actccaaagt ggatcgatat aatagtaata
     1201 gtaatcatcg tacccaatga gtaacgttac gtgaatattt gaaacttgtt tcgctggctt
     1261 gttatcaaag cggaaaacac gtagtaatgg tttagaacca agattagtat ggtatattaa
     1321 cacaggttga ccttgatcga taataccttt aagatcttct aacgatttac cagtgccgtc
     1381 tacgatatta ggattgtatt tttgtaaaaa tggtacatat gcttctggaa atatcgtttg
     1441 atgataattg ccaagcttaa tgaataagtg atgtccaaca taacctttat gtggattgtt
     1501 cggatgtgtc ggccaatgtc tcataatttc tgtagcaggg atatgttggt tgttgtattg
     1561 caacatcatg gctgcggaaa caccttcaca ccccatgacc atagggatag gaaatagctg
     1621 actgataggt ttaactggta atatttttcg gttcataata tagtcctcgc attgattcaa
     1681 taaatattta atataattat atagcgtcaa tgcaaaatgt cctaaacata tgttttacat
     1741 gagtgaataa aattaatgga gtgataaaat ggaatatcaa ttacaacaac ttgcgtcgtt
     1801 aacgttagta ggtattaaag aaacgtatga aaatggacga caggctcagc aacatatagc
     1861 agggttttgg caaagatgtt atcaagaggg agtaattgcg gatttacagt taaaaaataa
     1921 tggtgattta gccgggatac ttggcttatg tatacctgaa ttagacggta agatgtcata
     1981 tatgattgca gttaccggag ataatagtgc tgatattgaa aaatatgatg tcataacatt
     2041 agcaagttca aagtatatgg tatttgaagc acagggcgca gtacctaaag cagttcaaca
     2101 aaaaatggaa gaggttcatc actacataca tcaatatcaa gcagatacgg taaaatcagc
     2161 accatttttt gagttgtatc aggatggtga tactacaagt gaaaaatata ttacagaaat
     2221 ttggatgcct gttaaagggt gattgaatag aaatgtcctt gctatgaagg cgtaatttta
     2281 gttgaagatt cgatctcata attcataaag aagaaccctg acaaagatag atatctttgc
     2341 tagggttcta attatgttga agtgttatgc gacatgttgc tttttaatca ttttaaagta
     2401 aatgatttgt agactgacta atagaatggc tgtaatgaag ataataatca tatatggtga
     2461 tgtattaaat tcgcctttta agccaactaa tggtgcaaca gcgccaccta agataaattg
     2521 gaataagcca agtaaacttg atgcgttacc actgccacct gttcgttctt ccatagccat
     2581 tgtgaaacca agcggtccaa ttgacgtcac aggacacaca tttaagaaga atgcgatgag
     2641 taagacccaa agtggtaaat ggaatgtaag tgtcaggata attaaagcaa cacctaccac
     2701 ttgtataata gttaaaatga ttaataatat gtggcgatgt aatttttcta ctaataaagc
     2761 aacgacttga ctgacaatga ttaaacctac accgttaaca gcaaacatga tactaaattg
     2821 ttggggtgtc atattataca atttttgagt aataaatggc gatgcagatg aataactaaa
     2881 tagcattaca taagttaaac cttgtaatag cattggaata ataaatgctg gttttttcaa
     2941 aagacttcca aaatctttaa tgacgctact aaaattcacc tgctttaatt catctttaga
     3001 tgttttaggt aattgagaag cgacgccaat taaaatgatg agtgccacaa tagttaaaat
     3061 tgtgaaaata gaacgccatg ttgctacgga taaagctaat ccaccggcta atggtgcaag
     3121 aatagtgatg atgccattaa ctaccattaa agatgctaaa aatttagcga gtgcgttgcc
     3181 actaaattta tcaccagcag aggcttttgc aatcacgatg acgccaccac cagttaaacc
     3241 ttgaataaat cgtaaagtta agaataatgg caattgatca acaaacatag acaaacctga
     3301 taccaaaatg aaaatgatca aaatggatac agcaatccgt ttgcgaccaa aagcatcgga
     3361 taaaggtcca aagataaatt ggcctagtgc aagaccaatc attgtgaatg ataatgttaa
     3421 ttgaatttct gatgtagtag aaccaaaatc accttgcact ttaggtaatg atggtacgta
     3481 catatctata gttagtgggc caaaagcggt catgacgcct aacgtaatta gcaataaaaa
     3541 tgaaaaatgc ttcgttttgg ttttcgttga ttgcatgatc agactccttt aaatgatata
     3601 attatataaa ttatatacgt tttgtgtgta tttgtatgta gttagaatac aagtttatat
     3661 aaaaaatccc tatactctta atgcatcatc acgaataacg tagtgataca gataaagtat
     3721 agggaattaa tgtttatcat caaagcaggt gtaataatta attagcgcca ccattgtcat
     3781 ctttaaataa gctttctaat ttagatttaa tactgtcgaa ttttgtatta atatcattta
     3841 acatgctttc aattttacct tggcttgcat tgtttttaag tgcattattt gcctctgtca
     3901 atttgttgat ttggtcttga taaaattgag ttagcttaga atcagctttg ttttgttctt
     3961 gctgcttcaa gttgtcgatt tgattttgta aatttttaat aatgtcatca gttttttggt
     4021 tgtttttaat attttcttta gcattatcta cttgctggtt aatttggccg tcttgatttt
     4081 caacttgttt tttataattt tgagagactt tagggtcatt actaacttta tcgctatcat
     4141 tgttaagtaa ggcgcgtgtg acaaaaatag cgacaataat taagagaatg gcaataatga
     4201 tatataacca tttttttgaa cggcgggatt tctcgttttc ttcttcaaga atttgttctc
     4261 ttcgataatc tctaccatta tagtatcgtt ccgggtgttc ttcaggatcg cctttagcat
     4321 attgttgtct atacgatgcg tcttcagatt gacgacgatg attttggttt tgattgtcgt
     4381 attgatatga atcacggtat ttatcagttc ttttcatggg tttcacctca ttcaagtttt
     4441 aactgcaaat aataaagctg atgatttact ttgacattat tgtaatttca gaatacgagc
     4501 aatgtcagtt gcagtgttta ataaattttg ttcgctattt tgtaggacat cttctaaagt
     4561 gctaggtgaa gagattatag aataggcact atcaataccg aaatcgtaaa catgttgata
     4621 attttcgcct agactgccac aaatcgcgat gacaggaata tgatattgtt ttgcagctaa
     4681 cgcaacgcct acgggtgttt taccaaagat ggtctgataa tccatgcgtc cttctccagt
     4741 aataacgagg tctgcatctt taattctttg atgaaaatct gtaatgtcaa agacgacatc
     4801 aatacctttt gttaaagttg tctcacaaaa cgctaataat gctgcgccca taccgccagc
     4861 tgcaccagaa cctggtattt gattaacgga ctttcctgtg cacattttta tcttatcatg
     4921 ataatgcgac attgcgaaat ccaactttgg tatcatcttt gcatcagcgc ctttttgagg
     4981 accataaata taggtagcac cattttcacc caataaagga tttgaaacat cacaggccac
     5041 tttaaaggtc acctctttta atcgcgaatc tagattggtt atatcgattt gtgcaatgtg
     5101 agcaagatta gcaccattca tttgtaataa gtccccgttt acatcagtaa actttacgcc
     5161 tagtgcactt agcatacctg taccaccatc atttgttgca ctgccaccaa tccctaaaat
     5221 aatggtctta gcaccatgat ttaatgcatc tttaattagt tcaccggtac catatgatga
     5281 tgtgtataaa ggattacgtt cctctttttc taataaatcc aaacctgacg ctgccgccat
     5341 ttcaataatt gcaatttgtt gttcgtctgc gcgtgcataa catgcttcaa taggtcgcat
     5401 taaagggtca ttaacgatga ctgtatactt agtggcacct gttgcatgca ttaaagcatc
     5461 tgtggtacct tcaccaccat cagccatcgg aatgatatca taatgaaggg tattcccata
     5521 aacattagta aaagcctgtt ttataatatt gccaacttgc tgtgcggtca tactttcctt
     5581 aaatgagtca ggagcgatga caattttttg taattgcata gtagacatcc tctcattact
     5641 gttgtcgctc ttattgtata taaaattact agtgaactaa agtaatctct atagtgctaa
     5701 tgagtcacgc gataaatcag ttacattata atggcgtata aggatgaggg gaaagagact
     5761 aaatttgctg tgaaagggta taaagattga aataagcata tttgaagtga agcggattgg
     5821 ctcgcatatt cgagtatgtt cgagaacgta gttatgcata tattttgatg ttgaggagtt
     5881 acacatatga aactaacaca aacgcatgct gaaattttaa aatttatcat tgttggcggc
     5941 attaatacgt taaattatta tgttgtctat ttattgctgt taaaattatt acacattgaa
     6001 tatatgatta gtcatattac cggatttcta gtcgcttttg tgatttcata ttatttgaat
     6061 tgttattttg tttacagggt aaaacctact tggagaaaat tcattagttt tccaattacg
     6121 cagattgtca acgtaagctt acaaacagtt ttattatatg tatttgtatc ctggctaaat
     6181 ttgccagcag aaattgcacc atttgctggt ttaattatta cgatacccat cacatttata
     6241 ttatctaaat ggattttaaa agatagcaat taaagtagcg taaaccacta aaaaaacttt
     6301 acttaagcct taatattgcg tgaaatataa taaataaatg atataatatg atggtcgcct
     6361 aacatctcgg gcgttacttt aacgcagaga ggaggtgtat gacggatgtt caatttatta
     6421 attaacatca tgacttcagc tataagcggc tgtcttgttg cgttttttgc acattggtta
     6481 cgaacgcgca acaataaaaa aggtgacaaa taaccaacac ctaaataaat cccctcacta
     6541 tggcagtagt gaggggattt ggtgtattta gatgttcaat ttattatgta attaattata
     6601 gcattgatga acaaggtgtg caatgtattt ttgcaatatt acaataaatg aatttaatta
     6661 tattagaatt aagtatttcg aaaaatgatt taatagttga gtcataggat cattagataa
     6721 agcgaataat caattatagg gacattggga tacaaatcaa tgtactagac tctacaaggc
     6781 tatattcagg gacccaacaa agagaaattg gattcccaat ttctagagac aatgcaagtt
     6841 ggggtgggac gacgaaataa attttgtgaa aatatcattt ctgtcccact cccagaacca
     6901 ctacataata aatcattagt ggctctttat catttctgcc ccataacctt gtgaattttt
     6961 aaaagaaatg tgtcacaaat ccacgcgaca ttgataatcg ttatcaacta atatatgatg
     7021 tacttgtaat aagtattaat tttt
//
LOCUS       DAXWFK010000130         7041 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.130, whole genome shotgun sequence.
ACCESSION   DAXWFK010000130 DAXWFK010000000
VERSION     DAXWFK010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7041
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.130"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            99..1577
                     /gene="mqo"
                     /locus_tag="ACGEXS_002051"
     CDS             99..1577
                     /gene="mqo"
                     /locus_tag="ACGEXS_002051"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFF8013045.1"
                     /translation="MTTQHSKTDVILIGGGIMSATLGTLLKELSPEKNIKVFEKLAQP
                     GEESSNVWNNAGTGHSALCELNYTKEGKDGTVDCSKAIKINEQYQISKQFWAYLVKTG
                     QLDNPDRFIQAVPHMSFVIGEDNVAFIKSRVATLKKSILFEKMKLSQDEEEMKSWVPL
                     MIEGRKSDEPIALTYDETGTDVNFGALTAKLFDNLEQRGVGIQYKQNVLDIKKQKSGV
                     WLVKVKDLETNETTTYESDFVFIGAGGASLPLLQKTGIKQSKHIGGFPVSGLFLRCTN
                     QEVIDRHHAKVYGKAAVGAPPMSVPHLDTRFVDGKRSLLFGPFAGFSPKFLKTGSHMD
                     LIKSVKPNNIVTMLSAGIKEMSLTKYLVSQLMLSNDERMDDLRVFFPNAKNEDWEVIT
                     AGQRVQVIKDTEDSKGNLQFGTEVITSDDGTLAALLGASPGASTAVDIMFDVLQRCYR
                     DEFKGWEPKIKEMVPSFGYRLTDHEDLYHKINEEVTKYLQVK"
     gene            complement(1643..2098)
                     /locus_tag="ACGEXS_002052"
     CDS             complement(1643..2098)
                     /locus_tag="ACGEXS_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HFF8013046.1"
                     /translation="MKNLKNSLFISLIIGLSLSLFFSMLFADGKYYPLNPQSTIGILY
                     YTHFTETTVMLISIILWLLIGVVFFLGDFIFKYTDWSITKATIMHFITTYVGFLPLAM
                     LAGWFPLTVHYLIIFTIIFIVVYVLIWIIQFFKNKNYVDTINKQLKQLK"
     gene            complement(2095..2538)
                     /locus_tag="ACGEXS_002053"
     CDS             complement(2095..2538)
                     /locus_tag="ACGEXS_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HFF8013047.1"
                     /translation="MMKLNLFINANETESYIDIHAPKMNDNVQSIINAVNDLDKSHTL
                     VGYIDKEIHIINVSDVITFQVINKNVTAITSNQKFKLKLRLYELEKQLPQHFIRISKS
                     EIVNKYYIEKLLLEPNGLIRMYLKDAHYTYSSRRYLKSIKERLSI"
     gene            complement(2701..4074)
                     /gene="hssS"
                     /locus_tag="ACGEXS_002054"
     CDS             complement(2701..4074)
                     /gene="hssS"
                     /locus_tag="ACGEXS_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme sensor histidine kinase HssS"
                     /protein_id="HFF8013048.1"
                     /translation="MFKTLYARIAIYSITVILFSALISFVLTNVYYHYNLKASNDAKI
                     MKTLKEARQYEQSAKPTHIQQYFKHLGQMNYQIMTIDQKGHKTFYGEPFREDTLSQNA
                     INNVLNNQDYHGIKDKPFALFVTGFFDNVTDNTVGINFKTKDGSIAVFMRPDIGETFS
                     EFRTFLAVLLMLLLFISISLVIASTYSIIRPVKKLKLATERLIDGDFETPIKQTRKDE
                     IGTLQYHFNKMRESLGQVDQMRQHFVQNVSHEIKTPLTHIHHLLSELQQTSDKTLRQQ
                     YINDIYTITTQLSGLTTELLLLSELDNHQHLLFDDKIQVNQLIKDIIRHEQFAADEKS
                     LIILADLESINFLGNQRLLHQALSNLLINAIKYTDVGGAIDIALQHSHNNIIFTISND
                     GSPISPQAEARLFERFYKVSKHDNSNGLGLAITKSIIELHHGTIQFTQSNEYVTTFTI
                     TLPNNSL"
     gene            complement(4067..4741)
                     /gene="hssR"
                     /locus_tag="ACGEXS_002055"
     CDS             complement(4067..4741)
                     /gene="hssR"
                     /locus_tag="ACGEXS_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding heme response regulator HssR"
                     /protein_id="HFF8013049.1"
                     /translation="MVQCLVVDDDPRILNYIASHLQTEHIDAYTQPSGEAALKLLEKQ
                     RVDIAVVDIMMDGMDGFQLCNTLKNDYDIPVIMLTARDALSDKERAFISGTDDYVTKP
                     FEVKELIFRIRAVLRRYNINSNSEMTIGNLTLNQSYLELQVSNKTMTLPNKEFQLLFM
                     LAARPKQIFTREQIIEKIWGYDYEGDERTVDVHIKRLRQRLKKLNATLTIETVRGQGY
                     KVENHV"
     gene            4877..5932
                     /locus_tag="ACGEXS_002056"
     CDS             4877..5932
                     /locus_tag="ACGEXS_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8013050.1"
                     /translation="MKLAIKEIMFYKFRYILITLIILLLSIMVLFISGLAQGLGRENI
                     SLFEHFDNDEYVVQKMKEPQIEKSQLSDTQQNQIKKVIHQEPYKMNIQTLKLSNKEQD
                     VITMNDVKQQRIQLKKGDYPKNAHEVAINDKLAADNIRVGDRLHFKNNSTSYRVSGIL
                     NDTMYAHSSIVLLNDNGFNALNKVNTAFYPVKNLTQQQRDELNKINDVQVVSEKDLTG
                     NIASYQAEQAPLNMMIVSLFAITAIVLSAFFYVMTIQKISQIGILKAIGIKTRHLLSA
                     LVLQILTLTIIGVGIAVIIIVGLSFMMPVTMPFYLTTQNILLMVGIFILVAILGASLS
                     FIKLFKVDPIEAIGGAE"
     gene            5932..6597
                     /locus_tag="ACGEXS_002057"
     CDS             5932..6597
                     /locus_tag="ACGEXS_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8013051.1"
                     /translation="MALVVEDIVKNFGEGLSETKVLKGINFEVEQGEFVILNGASGSG
                     KTTLLTILGGLLSQTSGTVLYNDAPLFDKQHRPSDLRLEDIGFIFQSSHLVPYLKVIE
                     QLTLVGQEAGMTKQQSSTRAIQLLKNIGLEDRLNVYPHQLSGGEKQRVAIMRAFMNNP
                     KIILADEPTASLDADRATKVVEMIRQQIKEQQMIGIMITHDRRLFEYADRVIELEDGK
                     ITD"
     gene            complement(6705..7041)
                     /locus_tag="ACGEXS_002058"
                     /pseudo
     CDS             complement(6705..7041)
                     /locus_tag="ACGEXS_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT         2375 a          992 c         1281 g         2393 t
ORIGIN      
        1 ggaacctatt gagacactta ttgatttaaa gtggtattaa tatgtcgtat ttctcgaacg
       61 ttccattatt cattttaaaa agggggactg tatttgttat gacaacacaa catagcaaaa
      121 cagatgtcat cttaattggt ggcggtatta tgagtgcaac attaggaaca ttacttaaag
      181 aattatcacc tgagaaaaat attaaagtgt ttgaaaaatt agcacaacct ggcgaagaga
      241 gttcaaatgt atggaataat gccggtacag ggcattcagc actttgcgag ttgaactata
      301 caaaagaagg taaggatggc acagttgatt gtagtaaagc aattaagata aatgagcagt
      361 accaaatttc aaaacagttt tgggcatatt tagttaaaac aggacaatta gataacccag
      421 atcgctttat tcaagcggtg ccacacatga gttttgtcat tggcgaagat aatgtagctt
      481 ttataaaaag tcgtgttgca acgttaaaga aaagtatttt attcgaaaaa atgaaattat
      541 cgcaagatga agaagaaatg aaatcttggg taccgttaat gattgaaggt cgtaagtctg
      601 atgaaccaat tgctttaact tatgatgaaa ctggtacaga tgttaacttt ggtgcgttaa
      661 ctgcaaagtt atttgataat ttagagcaac gtggtgtggg aattcaatat aagcagaatg
      721 tattagacat caagaaacag aaatctgggg tatggctagt taaagttaaa gatttagaaa
      781 ctaatgaaac gacaacatat gaatctgatt ttgtatttat tggtgctggc ggtgcgagtt
      841 taccattact ccaaaagact gggattaaac aatcaaaaca tattggtggt ttcccggtaa
      901 gtggattatt cctgcgctgt acaaatcaag aagtgattga tcgtcatcat gctaaagtgt
      961 acggaaaagc agcagtgggt gcgccaccaa tgtcagtgcc gcacttagat acacgttttg
     1021 tagacggcaa gcgttcattg ttatttggtc catttgcagg tttctcacct aaatttttaa
     1081 aaacaggttc acatatggat ttaattaaat cggttaaacc aaataatatc gtgacgatgt
     1141 tatctgcagg tatcaaagaa atgagtctta cgaagtattt agtgtcacaa ttgatgttat
     1201 ctaatgatga gcgtatggat gatttaagag tctttttccc aaatgctaaa aatgaagatt
     1261 gggaagtgat tacagcaggg caacgtgtcc aagtaatcaa ggatactgag gattctaaag
     1321 gtaacttaca atttggtact gaagttatta cgtcagatga tggcacatta gctgcattac
     1381 ttggtgcatc acctggtgcg tcaacagctg tagatattat gtttgatgtt ttacagagat
     1441 gctatcgtga tgaattcaaa ggatgggaac caaagattaa agaaatggtg ccgtcatttg
     1501 gttatcgctt aacagatcat gaggatttat atcataaaat taatgaagaa gtaactaagt
     1561 atttacaagt taaataataa acgaaacggt aatgtctttt ttaatgtgat agacattacc
     1621 gttttttagt ggttaataaa aatcatttta attgtttcag ttgcttgtta atagtgtcta
     1681 cgtagttctt gtttttaaag aattgaatta tccaaattaa tacataaacc acaatgaaga
     1741 taattgtgaa tatgattaga taatgcactg ttagtggaaa ccaaccggca agcattgcta
     1801 aaggcaagaa tccgacatac gttgttatga aatgcattat agttgcttta gtaatgctcc
     1861 aatctgtgta tttaaagata aaatctccaa ggaaaaagac gacgcctatg agtaaccata
     1921 aaatgataga aatcaacatt acggtagttt ctgtgaaatg cgtataatac aatatgccaa
     1981 tagttgattg tgggttcagt ggataatatt tgccgtctgc aaataacata ctaaagaaca
     2041 gtgaaaggga caaaccaatg attaagctaa taaataatga gtttttcaaa tttttcatat
     2101 tgataagcgc tcctttatag attttaaata acgtctagaa gaataggtgt agtgtgcatc
     2161 tttaagatac atacgtataa gtccatttgg ctctaataat aatttttcaa tgtaatactt
     2221 gttgacgatt tctgatttgg aaatgcgaat gaaatgttgt ggtaactgtt tttctagttc
     2281 ataaagtcgt aattttagtt tgaatttttg attgcttgta atggctgtta catttttatt
     2341 aataacttgg aatgttataa catctgatac attaatgata tggatttctt tgtctatgta
     2401 tccgactaat gtatgtgatt tgtctaaatc attgactgca ttaataatac tttgaacgtt
     2461 atcattcatt ttaggtgcat gtatatcaat ataagattcc gtctcatttg cattgataaa
     2521 taaattgagt ttcatcatag gttaatgcct ccttcaaaat tattaaacca taaatgacca
     2581 tcgatatatt taaattttgt tgaatggtag aaattaaatg ttaagtggct agaaagcgct
     2641 aatcaatata aaagatacct cctgaaataa aaacagaaat gttttttcag gaggtagaga
     2701 ttaaagtgaa ttatttggca gtgtaatagt aaaggtggtt acatactcgt tactttgtgt
     2761 gaattggatt gtaccatgat gcaattcaat gatggatttt gtaattgcaa gacctaaacc
     2821 attgctatta tcatgtttgc tcactttata aaaacgttca aataaacgtg cttcagcttg
     2881 tggactaatt ggtgaaccat cattacttat tgtgaaaatg atattgttgt gactatgttg
     2941 caaagcgatg tcaatggcac caccaacatc tgtatactta atagcattta ttaataaatt
     3001 actcaatgct tgatgtaaca aacgttgatt tcctaggaaa ttgattgatt ctaggtcagc
     3061 taaaatgatt aacgactttt catcagcagc aaattgttca tgtcgaatga tatctttaat
     3121 gagttgattg acttgaatct tatcatcgaa taataaatgt tggtgattat ctaattcgga
     3181 taagagcaac aattcagttg ttaatccgct cagctgcgtt gtaatggtat agatatcatt
     3241 tatatattgt tgtcttaatg ttttatcaga agtttgttgt aactcgctta ataaatgatg
     3301 aatatgtgtt aatggtgttt tgatttcatg agatacattt tgtacaaaat gctgcctcat
     3361 ttgatcaact tgtcccaatg actctctcat cttattaaag tgatactgta atgttccaat
     3421 ttcatctttg cgtgtttgtt tgataggtgt ttcaaaatca ccatcaatta agcgttcggt
     3481 cgctagcttt aactttttta ctggacgaat gattgaatag gttgatgcga taactaatga
     3541 aatcgagata aataataaca acattaacaa gacggctaag aatgttctaa actcgctaaa
     3601 tgtttctcca atatctggac gcataaatac tgctattgaa ccgtcttttg tcttaaagtt
     3661 aataccaaca gtattatctg ttacattgtc gaaaaatcca gttacgaata atgcgaatgg
     3721 tttatcttta ataccatggt aatcttgatt atttaatacg ttgttaatag cattttgtga
     3781 taaagtatct tctctaaatg gctccccata aaatgtctta tgaccttttt gatcaatggt
     3841 cattatttgg taattcattt gccctaaatg tttgaaatat tgttgaatgt gcgttggttt
     3901 agctgattgt tcatattgtc ttgcttcttt aagcgtcttc atgattttcg cgtcattaga
     3961 tgcttttaaa ttataatgat agtaaacatt tgttaataca aaacttatta atgcactaaa
     4021 taaaataacc gtaatggaat aaatcgcaat tctagcatag agtgttttaa acatgattct
     4081 ccaccttata gccttgtcct cttactgttt caattgtaag tgtggcattt aattttttta
     4141 atctttggcg tagtcgctta atatgaacgt caactgttcg ctcatctcct tcataatcat
     4201 agccccaaat tttttctatt atttgttcac gagtaaagat ttgcttagga cgtgctgcaa
     4261 gcataaataa taattgaaat tccttgttcg gtaacgtcat cgttttatta gatacttgga
     4321 gttccaaata ggattggttt agcgttaagt tgccaatagt catttctgaa tttgaattga
     4381 tattatatcg acgtaataca gcacgaattc taaaaataag ttccttaacc tcaaagggtt
     4441 tggttacata atcgtcagta ccgcttataa acgcacgctc tttgtcacta agtgcatccc
     4501 gcgctgttaa cataataact ggtatatcat aatcattttt taatgtatta cataattgaa
     4561 agccgtccat accatccatc ataatatcta ccactgcaat atcgacacgc tgtttttcta
     4621 aaagttttaa tgcagcttca ccacttggtt gtgtgtatgc atcaatgtgc tctgtttgta
     4681 aatggctagc tatataattt aggattcgag gatcgtcatc gacaacaaga cattgcacca
     4741 tagctataaa ctcccttatc tttttcattt attatacatg taaaatattt ttgcgtaaaa
     4801 aaacaattgt tcatattgag ttcatatttc aaccttatac tgacgctaaa gaagaaatag
     4861 ggagaagtga atcgatatga aattagcgat aaaagagatt atgttttaca aatttcgtta
     4921 tattttaatc acattaatca ttcttttatt aagtattatg gtgttattta ttagtggttt
     4981 agctcaaggg cttggtaggg agaatatttc gttatttgaa cactttgata atgatgaata
     5041 tgttgttcaa aaaatgaaag agccgcaaat tgagaaatcg caacttagtg atacgcaaca
     5101 gaatcaaatt aaaaaggtaa ttcatcaaga accatataaa atgaatattc aaacattgaa
     5161 attatctaat aaagaacaag atgtgattac aatgaatgac gttaaacaac aacgcataca
     5221 acttaaaaaa ggtgattatc ctaaaaatgc tcatgaggtc gctattaatg ataagttagc
     5281 tgcagacaac attagagtcg gggatagatt acattttaaa aataattcaa ctagttatag
     5341 agtttctggt attttaaacg acacaatgta tgcgcatagt tccattgtgc tattgaacga
     5401 taacggattt aatgcattga ataaggttaa tacggcattt tatccagtga aaaatttaac
     5461 acaacaacaa cgtgatgagc ttaataaaat aaatgacgtt caagttgtga gtgaaaaaga
     5521 tttaacaggt aatattgcga gttatcaagc agagcaagca ccgttaaata tgatgattgt
     5581 tagtttgttt gctattacag caatcgttct aagtgcattt ttctatgtta tgacgattca
     5641 aaaaatatca caaattggca ttttgaaagc aattggtatt aagacaagac atttattgag
     5701 tgcgttagtt ttacaaattt taacactaac aataattggg gtaggtattg ctgtgatcat
     5761 catagtagga ctatcattta tgatgccggt aacgatgcct ttttacttaa caacgcaaaa
     5821 tattttatta atggtgggga tatttatatt agtagcgatt ttaggtgcct cactatcatt
     5881 tatcaaatta tttaaagtgg atcctatcga agcaattgga ggtgcagaat aatggcatta
     5941 gtcgttgaag atatcgtcaa aaatttcgga gaaggtttgt ctgaaacaaa agttttaaaa
     6001 ggtattaatt ttgaagtgga acaaggggaa tttgtcattt taaatggtgc ctctggttct
     6061 gggaaaacaa cattgctaac gatattaggc ggattgttaa gtcaaacgag tggtacagtg
     6121 ctttacaatg atgcgccatt gtttgataaa cagcatcgtc ctagtgattt acgattggaa
     6181 gatattggtt ttatttttca atcttcacat ttagttcctt atttaaaagt gatagagcaa
     6241 ttgacactcg taggtcaaga agcgggaatg accaaacaac aaagttcaac aagagcaata
     6301 caacttttga aaaatattgg tttagaagat cgcttgaatg tatatccgca tcagttatct
     6361 ggcggtgaaa agcaacgtgt tgcgattatg agagcattta tgaataatcc gaaaatcatt
     6421 ttagcagatg agcccacagc aagtttagat gccgatagag caacaaaagt tgttgagatg
     6481 atacgtcaac aaattaaaga acaacaaatg attggtatta tgattacaca cgatcgaaga
     6541 ttatttgaat atgcagatcg agtgattgaa ttagaagatg gcaaaataac tgattagtgg
     6601 cttgtaaaga cgctaaatgt taatgattta agacatagta gtataaaagt tagataacag
     6661 aatacgattt gggtttacaa aaaacaggct gggacattaa gttcttaggc aatgtaaaaa
     6721 agctgatttc tattaattat ttgatagaaa tcagcttttt tgatatgtat tttataatgt
     6781 acagctcgtt gcattcatat agcttgaagt cacgtttaaa accatatcta tcattatggt
     6841 atgcatatct tttaaaacct attcttttgt tattaggaca tataaattca tcattaagtt
     6901 cgtcatattt ccaattttga gtgttaaaaa tgtcactttt aaactttcta gttttatctt
     6961 taataaacat gacatacgta ataagtggcg ttttattaaa atcatctata atagccatat
     7021 agttttgctc actatcataa c
//
LOCUS       DAXWFK010000131         6921 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.131, whole genome shotgun sequence.
ACCESSION   DAXWFK010000131 DAXWFK010000000
VERSION     DAXWFK010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6921
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.131"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            113..1015
                     /locus_tag="ACGEXS_002059"
     CDS             113..1015
                     /locus_tag="ACGEXS_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFF8013052.1"
                     /translation="MKFLSFKYNDKTSYGVKVKREDAVWDLTQVFADFAEGDFHPKTL
                     LAGLQQNHTLDFQEQVRKAVVAAEDSGKAEDYKISFNDIEFLPPVTPPNNVIAFGRNY
                     KDHANELNHEVEKLYVFTKAASSLTGDNATIPNHKDITDQLDYEGELGIVIGKSGEKI
                     PKALALDYVYGYTIINDITDRKAQSEQDQAFLSKSLTGGCPMGPYIVTKDELPLPENV
                     NIVTKVNNEIRQDGNTGEMILKIDELIEEISKYVALHPGDIIATGTPAGVGAGMQPPK
                     FLQPGDEVKVTIDNIGTLTTYIAK"
     gene            1344..1733
                     /locus_tag="ACGEXS_002060"
     CDS             1344..1733
                     /locus_tag="ACGEXS_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YisL family protein"
                     /protein_id="HFF8013053.1"
                     /translation="MLHLHILSWVLAIILFIATYLNISKNQGRSPFFKPLHMILRLFM
                     LLTLISGFWILIQSFMNGGANHMLLTLKMLCGVAVVGLMEVSIAKRKRHEQSHTMFWI
                     TIALIIITMVLGVILPLGPISKLFGIG"
     gene            complement(1904..3220)
                     /locus_tag="ACGEXS_002061"
     CDS             complement(1904..3220)
                     /locus_tag="ACGEXS_002061"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HFF8013054.1"
                     /translation="MPKIVVVGAVAGGATCASQIRRLDKESDIIFFEKDRDMSFANCA
                     LPYVIGEVVEDRRYALAYTPEKFYDRKQITVKTYHEVIAINDERQTVSVLNRKTNEQF
                     EESYDKLILSPGASANSLGFESDITFTLRNLEDTDAIDQFIKANQVDKVLVVGAGYVS
                     LEVLENLYERGLHPTLIHRSDKINKLMDADMNQPILDELDKREIPYRLNEEINAINGN
                     EITFKSGKVEHYDMIIEGVGTHPNSKFIESSNIKLDRKGFIPVNDKFETNVPNIYAIG
                     DIATSHYRHVDLPASVPLAWGAHRAASIVAEQIAGNDTIEFKGFLGNNIVKFFDYTFA
                     SVGVKPNELKQFDYKMVEVTQGAHANYYPGNSPLHLRVYYDTSNRQILRAAAVGKEGA
                     DKRIDVLSMAMMNQLTVDELTEFEVAYAPPYSHPKDLINMIGYKAK"
     gene            complement(3272..4096)
                     /locus_tag="ACGEXS_002062"
     CDS             complement(3272..4096)
                     /locus_tag="ACGEXS_002062"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFF8013055.1"
                     /translation="MQPHLICLDLDGTLLNDNKEISSYTKQVLNELQQRGHQIMIATG
                     RPYRASQMYYHELNLTTPIVNFNGAYVHHPKDKNFKTCHEILDLGIAQNIIQGLQQYQ
                     VSNIIAEVKDYVFINNHDPRLFEGFSMGNPRIQTGNLLVHLKESPTSILIEAEESKIP
                     EIKNMLTHFYADHIEHRRWGAPFPVIEIVKLGINKARGIEQVRQFLNIDRNNIIAFGD
                     EDNDIEMIEYARHGVAMENGLQELKDVANNITFNNNEDGIGRYLNDFFNLNIRYYC"
     gene            4210..4518
                     /locus_tag="ACGEXS_002063"
     CDS             4210..4518
                     /locus_tag="ACGEXS_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFF8013056.1"
                     /translation="MEEALKDSILGALEMVIDPELGIDIVNLGLVYKVNVDDEGVCTV
                     DMTLTSMGCPMGPQIIDQVKTVLAEIPEIQDTEVNIVWSPPWTKDMMSRYAKIALGVS
                     "
     gene            5065..6879
                     /locus_tag="ACGEXS_002064"
     CDS             5065..6879
                     /locus_tag="ACGEXS_002064"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFF8013057.1"
                     /translation="MNKTKGFTKYKKMRYIPGLDGLRAIAVLGIIIYHLNKQWLTGGF
                     LGVDTFFVISGYLITSLLLKEYDDTGIIKLKSFWIRRLKRLLPAVIVLLMVVGTATLL
                     LKSDNIIRVKHDIIAAIFYVSNWWYIAKDVNYFEQFSFMPLKHLWSLAIEEQFYIFFP
                     VILVTLLLTIKKRYKIGFIFWGVSIISLGLMMFIYSINGDHSRVYFGTDTRLQTLLLG
                     VILAFLWPPFKLKNDPPKVVKYVIDSIGSLSFIVLILLFFIINDETNWIYDGGFYLIS
                     ILTLFIIASVVHPSTWIAKIFSNPVLVFIGKRSYSLYLWHFAVISFVHSYYVDGQIPV
                     YVYFIDISLTIIFAELSYRFIETPFRKEGIKALNWRPSYIPQFIRMAIVVTLLIPFML
                     ILVGAFNKYGKDIIGEKANSFDTTIEDNYLMRIAPIDNIHIDGLVSEKKKESSDVYNN
                     IKPLLIGDSVMVDIGESFKSSVPKSRIDGKVGRQLYQTLPLVKANYSQYKKSSDQVVL
                     ELGTNGDFTVKQLDDLLNQFGKAKIYLVNTRVPRIYEANVNRLLADAAKRKSNVTLID
                     WYKRSQGHSEYFAPDGVHLEYKGVLALKDEILKALKKK"
BASE COUNT         2325 a          993 c         1182 g         2421 t
ORIGIN      
        1 gtagcagaat gaacacaact cattttcaaa attgtcttac ttatttattt gttatttgat
       61 aacgaaaaaa gttataatgt gaattaagat aaagatgagg agttgagaat gaatgaaatt
      121 cttatcattc aagtataatg acaaaacttc atatggcgtt aaagtaaaac gcgaagatgc
      181 tgtatgggat ttaacacaag tatttgctga ctttgcagaa ggagatttcc atcctaaaac
      241 attgttagct ggtttacaac aaaatcatac tttagatttt caagaacaag tacgtaaagc
      301 agttgtagca gcagaagata gcggcaaagc tgaagactat aaaatttcat ttaatgacat
      361 tgaattttta ccaccagtaa cacctccgaa taatgtgatt gcttttggta gaaattacaa
      421 agatcatgcg aacgaattaa atcatgaagt ggaaaaatta tatgtattta caaaagcagc
      481 gtcatcttta acaggagata atgcaacaat tccaaatcat aaagatatta ctgatcaatt
      541 agattatgaa ggtgaattag gtattgttat tggtaagtct ggtgaaaaga ttccaaaagc
      601 attagcttta gattatgttt acggctatac aattattaac gatatcactg atcgcaaagc
      661 acaaagtgaa caagatcaag catttttatc aaaaagttta actggcggtt gcccaatggg
      721 tccttatatc gttactaaag acgaactacc attacctgaa aatgtaaata ttgttacaaa
      781 agttaacaat gaaattagac aagatggtaa cactggcgaa atgattctta aaattgatga
      841 attaatagaa gaaatttcaa aatatgttgc actacatccg ggagatatta ttgcaactgg
      901 tacaccagca ggcgttggtg caggtatgca accacctaaa tttttacaac caggtgatga
      961 agttaaagtg actattgata atattggaac gctgacaact tatatcgcta aataattatc
     1021 atttaaaaag ctaaccagtc tttatataga ttggttagtt ttttcttgct tttctaaaaa
     1081 ggtgttaaag ataaattatt tataatgtta ccattttgag atgaaagtga aatattgata
     1141 ttaagaagta gttgattatt ttacagcaga ttcacaatat tctaataagg aataaaatgt
     1201 catgttcttc ctctcaaata tagaagtgtg gtagaatata tattcgtgta taatcaaatc
     1261 atgattaaat tacaagcaag tgggtattaa tcccaagaag ctttaattta ataatataaa
     1321 taattaaagt ggggggaatc agtatgttac atttacatat attaagttgg gtattagcga
     1381 ttattttatt tatcgctaca tacttaaaca tttcaaaaaa tcaaggcaga tcaccatttt
     1441 tcaaaccgtt gcacatgatt ttacgcttat ttatgctgtt gacgttaatt tcaggatttt
     1501 ggatattaat tcagtcattt atgaatggcg gggcaaatca tatgttgctt acattgaaaa
     1561 tgctgtgtgg tgttgcagta gttggattga tggaagtgtc gattgctaaa agaaagagac
     1621 atgaacaaag tcacacaatg ttttggataa caattgcatt aattatcatc acaatggtat
     1681 taggtgtcat tctaccgtta gggcctatat caaaattatt cggtattggc taattgataa
     1741 gacatatagt atatgacata aggcatggac aattattagt ccaagacctt atgtcttttt
     1801 tgattctaaa aatgttaaac ataaaaaatg gactggatag tagtaccagt ccaacataat
     1861 tctaacctag acaattaaga ttagattgaa tcgcatgatt catttattta gctttgtaac
     1921 caatcatatt gattaaatct ttagggtggc tatatggtgg tgcataagcc acttcaaact
     1981 cagttaactc atctacagtt agctggttca tcattgccat cgatagtaca tcaatacgtt
     2041 tatctgcacc ttcttttcct actgcagctg ctcttaaaat ctgacggttt gaagtgtcat
     2101 aatatactct taagtgtaaa ggggaatttc ctgggtaata attcgcgtgt gcaccttgag
     2161 tgacttctac cattttatag tcaaattgct ttagttcgtt tggtttaacg ccgacactcg
     2221 caaatgtata atcaaagaac ttcacaatat tgttgcctaa gaagcctttg aattcaatag
     2281 tgtcatttcc agcaatttgt tcggcaacaa tacttgctgc acggtgagcg ccccaagcta
     2341 aaggaacact agccggtaga tcgacatgtc gataatgtga tgttgcaata tcgcctattg
     2401 cataaatgtt tggaacattt gtttcaaatt tatcgtttac cggtatgaaa ccttttcgat
     2461 caagtttgat atttgaactt tcgataaatt ttgaattggg gtgagtaccg acaccttcaa
     2521 taatcatatc gtaatgttca acttttcctg atttaaatgt aatttcattt ccattgatag
     2581 cattaatttc ctcatttaaa cggtatggaa tctcccgctt atctaattca tcaagtatag
     2641 gttgattcat gtcggcatcc attaatttat ttatcttatc agatcgatga attaaagtag
     2701 ggtgtaaacc acgttcataa agattttcaa gaacttctaa tgaaacataa cctgcaccta
     2761 caaccaatac tttatcaact tgatttgctt tgatgaattg atcgatagca tcagtgtctt
     2821 ctaaatttct aagtgtaaat gtaatatcac tttcaaagcc aaggctattt gcacttgcac
     2881 cagggcttaa aatgagttta tcgtaagatt cttcaaattg ttcgtttgtc tttctattta
     2941 atacagatac agtttgtctt tcatcattga ttgcaataac ttcatgataa gtttttactg
     3001 taatttgctt tctatcataa aatttttcag gtgtatacgc taaagcatat cttctatctt
     3061 caacaacttc gccaatgaca taaggcaatg cacaattagc aaagctcata tcacgatctt
     3121 tttcaaaaaa aataatgtca ctttctttat ctaaacgtcg aatttggctg gcacatgttg
     3181 caccgccagc gactgctccg actacgacta ttttgggcat cgtaacttac ctcctattgt
     3241 tttaaaatat tataaaatga ttagttataa attaacagta atatctaata tttaaattaa
     3301 agaaatcatt caaatatcga ccaatgccat cttcattatt gttgaatgta atattgttcg
     3361 ctacatcttt aagttcttgc aaaccatttt ccatagcaac accgtgacgc gcgtactcaa
     3421 tcatttcaat atcattatct tcatcaccga atgcaataat attatttcgg tcaatattta
     3481 aaaattgtct aacttgctca atgcctcttg ctttattaat accaagtttt acaatttcaa
     3541 tgacagggaa tggtgcgccc cagcgtcgat gctcaatatg atcggcataa aaatgagtaa
     3601 gcatattttt gatttcaggt attttacttt cttcggcttc aattaaaatt gaggtagggg
     3661 attctttcaa gtggacaagt aaattaccag tttgaattct tggattaccc attgaaaaac
     3721 cttcaaataa tcttggatca tgattgttaa tgaaaacata atctttcact tctgctataa
     3781 tattcgatac ttgatattgt tgtaatcctt gaataatgtt ttgtgcgatg cctaaatcta
     3841 aaatttcatg gcaagttttg aagtttttat ctttagggtg atgtacgtaa gcgccattaa
     3901 aattaacaat tggtgtcgtt aaatttaatt catgataata catttgactt gcacgataag
     3961 gtctgccagt cgcaatcata atttggtgtc cacgttgttg taattcattt aatacttgtt
     4021 tagtatatga tgaaatttct ttgttatcgt ttaataatgt tccgtctaag tctagacata
     4081 ttaaatgtgg ttgcatatct ctctcctcat aagttgctta gttcatatag atacatcata
     4141 gcattttatt ggcgttttgt cggatatttg atatattatg aataatatga cattaaagag
     4201 gtgatatgga tggaagaggc attgaaagat agtatcttag gtgcattaga aatggtaatt
     4261 gaccctgaat taggaattga tatcgttaat ttgggtttag tatacaaagt gaatgttgat
     4321 gatgaaggcg tatgtacagt tgatatgact ttaacatcaa tgggatgtcc aatgggacct
     4381 caaattattg atcaagttaa aacagtatta gcagagattc ctgaaattca ggatactgaa
     4441 gtgaatatcg tatggagtcc accttggaca aaagatatga tgtcacgtta cgctaagatt
     4501 gcacttggtg tgagctaaca taaacttgca ttgcctgtag aatttctttt cgaaattctc
     4561 tgtgtttggg cccgcagtca atataacatt gtagagtata attcattgtg aataagctcc
     4621 cttcaaagta gacattgaaa aatgaaaatt ttgaagggag ccttatttat gttctagttt
     4681 ctgattttat tttgaaaatt tatatctaac ttgtttcaaa aaatatgaat taagacagtg
     4741 acttgcactt ccaagagtgc acaattcatt ttctagttga atattttctt gaaaattttt
     4801 ataataaatt gaatttatat tacagttata ttacaataca taatacttca tagtttttaa
     4861 gttgccgagt tgcattacaa attggatttc gaagtaagaa tcatcattgg taattttttt
     4921 aatttaacag tattttagtc aatatgattt tgatgatgtc gtaattcaat aatatacata
     4981 tgcatttcaa tattctttta aacagcataa ctaaatttaa ttaagcatct aagaagaaca
     5041 gttttaaaag ggaagaatgc aaaaatgaac aaaacaaagg gttttacaaa gtataagaaa
     5101 atgagatata ttccagggct cgatggtttg agggcaatcg ctgttctagg aattattatt
     5161 taccacttaa ataagcaatg gttgacaggt ggctttttag gtgtggatac attttttgtg
     5221 atctctggtt atttaattac aagcttatta ctcaaagagt atgatgacac aggtatcatt
     5281 aaattgaaaa gcttttggat acgtcgttta aaacgtttat taccagcagt catagtttta
     5341 ttaatggttg tagggacagc aaccttatta ttaaaatcag ataatatcat tagggttaaa
     5401 catgatatta ttgctgcgat attttatgta tcaaactggt ggtatatagc aaaagatgtt
     5461 aattattttg agcaattttc atttatgcca ttaaagcatt tatggtcttt agcaattgaa
     5521 gaacagtttt acatattttt cccagttatt ttggttacat tattgttaac aattaaaaag
     5581 cgatacaaaa taggatttat tttttgggga gtatcaataa tttctttagg gttaatgatg
     5641 tttatctata gtattaatgg ggatcattca cgagtgtatt ttggtacaga tactagatta
     5701 cagacattgt tactgggtgt tattttagct tttttatggc caccgtttaa attgaaaaat
     5761 gatccaccta aagttgtaaa atatgttatt gatagcatag gtagtttatc atttatagta
     5821 cttatattat tatttttcat tattaatgat gagacgaatt ggatatatga tggtggtttc
     5881 tatttaatat ccatattaac gttatttatt attgctagtg tcgttcatcc atctacatgg
     5941 atagcgaaga tattttcaaa tccagtgtta gtatttatcg ggaaaaggtc ttatagttta
     6001 tatttatggc attttgcagt aattagtttc gtacatagtt actatgtaga cggacagata
     6061 cctgtatatg tgtactttat agatataagt ttaacaatta tatttgcaga gctatcatat
     6121 cgctttatag aaactccatt tagaaaagaa ggtattaaag ctttaaattg gcgaccttct
     6181 tatataccac aatttataag aatggcaatt gtagtaacct tgttaattcc atttatgttg
     6241 attttagtag gtgcattcaa taaatatggt aaagacatta ttggagaaaa agcgaatagc
     6301 tttgatacca caattgaaga taattattta atgcggatag caccaattga taacatacac
     6361 attgatggct tagtaagtga gaagaaaaag gaatcttccg acgtatataa taatattaaa
     6421 cctcttttaa tcggtgattc agtaatggtt gatatcggtg agtcatttaa gtcatcagtt
     6481 cctaagtcta gaattgatgg aaaagtaggg cgtcaattgt atcaaacctt acctttagtt
     6541 aaagcgaatt attcacaata taaaaaatca tctgatcaag tcgtattaga attaggtaca
     6601 aatggcgact ttactgtcaa acagctcgac gatttactta atcaatttgg aaaagccaag
     6661 atttatttag ttaatacacg tgttccaaga atttatgagg caaatgtaaa tcgattatta
     6721 gctgacgcgg cgaaacgaaa gtccaatgtc acattaattg attggtataa gcgatcacaa
     6781 ggacatagtg aatattttgc accagacggt gtacatttag agtacaaagg agtcctagct
     6841 ttaaaagatg aaatattaaa agcacttaaa aagaaataac atatgtttaa taagtctagt
     6901 taatgtgtaa cgtaacatta g
//
LOCUS       DAXWFK010000132         6892 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.132, whole genome shotgun sequence.
ACCESSION   DAXWFK010000132 DAXWFK010000000
VERSION     DAXWFK010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6892
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.132"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            8..667
                     /gene="arlR"
                     /locus_tag="ACGEXS_002065"
     CDS             8..667
                     /gene="arlR"
                     /locus_tag="ACGEXS_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor ArlR"
                     /protein_id="HFF8013058.1"
                     /translation="MTQILIVEDEQNLARFLELELTHENYNVDTEYDGQDGLDKALSH
                     YYDLIILDLMLPSINGLEICRKIRQQQSTPIIIITAKSDTYDKVAGLDYGADDYIVKP
                     FDIEELLARIRAILRRQPQKDIIDVNGITIDKNAFKVTVNGAEIELTKTEYDLLYLLA
                     ENKNHVMQREQILNHVWGYNSEVETNVVDVYIRYLRNKLKPYDRDKMIETVRGVGYVI
                     R"
     gene            664..2019
                     /gene="arlS"
                     /locus_tag="ACGEXS_002066"
     CDS             664..2019
                     /gene="arlS"
                     /locus_tag="ACGEXS_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase ArlS"
                     /protein_id="HFF8013059.1"
                     /translation="MTKRKLRNNWIIVTTMITFVTIFLFCLIIIFFLKDTLHNSELDD
                     AERSSSDINNLFHSKPVKDISALDLNASLGNFQEIIIYDEHNNKLFETSNDNTVRVEP
                     GYEHRYFDRVIKKRYKGIEYLIIKEPITTQDFKGYSLLIHSLENYDNIVKSLYIIALA
                     FGVIATIITATISYVFSTQITKPLVSLSNKMIEIRRDGFQNKLQLNTNYEEIDNLANT
                     FNEMMSQIEESFNQQRQFVEDASHELRTPLQIIQGHLNLIQRWGKKDPAVLEESLNIS
                     IEEMNRIIKLVEELLELTKGDVNDISSEAQTVHINDEIRSRIHSLKQLHPDYQFDTDL
                     TSKNLEIKMKPHQFEQLFLIFIDNAIKYDVKNKKIKVKTRLKNKQKIIEITDHGIGIP
                     EEDQDFIFDRFYRVDKSRSRSQGGNGLGLSIAQKIIQLNGGSIKIKSEINKGTTFKII
                     F"
     gene            2303..5101
                     /locus_tag="ACGEXS_002067"
     CDS             2303..5101
                     /locus_tag="ACGEXS_002067"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFF8013060.1"
                     /translation="MTNERKEVSEAPVNFGANLGLMLDLYDDFLQDPSSVPEDLQVLF
                     STIKNDDSIVPALKSTSSQNSDGTIKRVMRLIDNIRQYGHLKADIYPVNPPKRKHVPK
                     LEIEDFDLDQQTLEGISAGIVSDHFADIYDNAYEAILRMEKRYKGPIAFEYTHINNNT
                     ERGWLKRRIETPYKVTLNNNEKRALFKQLAYVEGFEKYLHKNFVGAKRFSIEGVDALV
                     PMLQRTITIAAKEGIKNIQIGMAHRGRLNVLTHVLEKPYEMMISEFMHTDPMKFLPED
                     GSLQLTAGWTGDVKYHLGGIKTTDSYGTMQRIALANNPSHLEIVAPVVEGRTRAAQDD
                     TQRAGAPTTDHHKAMPIIIHGDAAYPGQGINFETMNLGNLKGYSTGGSLHIITNNRIG
                     FTTEPIDARSTTYSTDVAKGYDVPIFHVNADDVEATIEAIDIAMEFRKEFHKDVVIDL
                     VGYRRFGHNEMDEPSITNPVPYQNIRKHDSVEYVFGKKLVNEGVISEDEMHSFIEQVQ
                     KELRQAHDKINKADKMDNPDMEKPADLALPLQADEQSFTFDHLKEINDALLTYPDGFN
                     ILKKLNKVLEKRHEPFNKEDGLVDWAQAEQLAFATILQDGTPIRLTGQDSERGTFSHR
                     HAVLHDEQTGETYTPLHHVPDQKATFDIHNSPLSEAAVVGFEYGYNVENKKSFNIWEA
                     QYGDFANMSQMIFDNFLFSSRSKWGERSGLTLFLPHAYEGQGPEHSSARLERFLQLAA
                     ENNCTVVNLSSSSNYFHLLRAQAASLDSEQMRPLVVMSPKSLLRNKTVAKPIDEFTSG
                     GFEPILTESYQADKVTKVILATGKMFIDLKEALAKNPDESVLLVAIERLYPFPEEEIE
                     ALLAQLPNLEEVSWVQEEPKNQGAWLYVYPYVKVLVADKYDLSYYGRIQRAAPAEGDG
                     EIHKLVQNKIIENALKNN"
     gene            5115..6383
                     /gene="sucB"
                     /locus_tag="ACGEXS_002068"
     CDS             5115..6383
                     /gene="sucB"
                     /locus_tag="ACGEXS_002068"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFF8013061.1"
                     /translation="MPEVKVPELAESITEGTIAEWLKNVGDSVEKGEAILELETDKVN
                     VEVVSEEAGVLSEQLASEGDTVEVGQAIAIIGEGSGNASKENSNDNTPQQNEETNNKK
                     EEITNNSVDKAEVNQANDDNQQRINATPSARRYARENGVNLAEVSPKTNDVVRKEDID
                     KKQQAPASTQTTQQASAKEEKKYNQYPTKPVIREKMSRRKKTAAKKLLEVSNNTAMLT
                     TFNEVDMTNVMELRKRKKEQFMKDHDGTKLGFMSFFTKASVAALKKYPEVNAEIDGDD
                     MITKQYYDIGVAVSTDDGLLVPFVRDCDKKNFAEIEAEIANLAVKAREKKLGLDDMVN
                     GSFTITNGGIFGSMMSTPIINGNQAAILGMHSIITRPIAIDQDTIENRPMMYIALSYD
                     HRIIDGKEAVGFLKTIKELIENPEDLLLES"
BASE COUNT         2594 a         1017 c         1249 g         2032 t
ORIGIN      
        1 tgtacaaatg acgcaaattt taatagtaga agatgaacaa aacttagcaa gatttcttga
       61 attggaactc acacatgaaa attacaatgt ggacacagag tatgatggac aagacggttt
      121 agataaagcg cttagccatt actatgattt aatcatatta gatttaatgt tgccgtcaat
      181 taatggctta gaaatttgtc gcaaaattag acaacaacaa tctacaccta tcattataat
      241 tacagcgaaa agtgatacgt atgacaaagt tgctgggctt gattacggtg cagacgatta
      301 tatagttaag ccgtttgata ttgaagaact tttagcaaga attcgtgcaa ttttacgtcg
      361 tcagccacaa aaggatatta tcgatgtcaa cggtattaca attgataaga acgcttttaa
      421 agtgacggta aatggcgcag aaattgaatt aacaaaaaca gagtatgatt tactatatct
      481 tctagctgaa aataaaaacc atgttatgca acgggaacaa attttaaatc atgtatgggg
      541 ttataatagt gaagtagaaa caaatgtcgt agatgtttat ataagatatt tacgaaacaa
      601 gttaaaacca tacgatcgtg acaaaatgat tgaaacagtt cgtggcgttg ggtatgtgat
      661 acgatgacaa aacgtaaatt gcgcaataac tggattattg ttaccacgat gattacgttt
      721 gtcacgatat ttttgttttg tttaattatt atttttttct tgaaagatac actgcataat
      781 agtgagcttg atgatgcaga acgaagctca agcgatatta ataatttatt tcattctaag
      841 cctgttaaag atatatctgc attagacttg aatgcatctt taggtaattt tcaagagata
      901 attatttatg atgagcataa taataaatta tttgagacat cgaatgataa cacagtgaga
      961 gttgaaccag gttatgaaca ccgttatttt gaccgcgtaa taaaaaaacg ctataaaggc
     1021 attgaatatt taattattaa agaaccaatt acaacgcaag atttcaaagg gtatagcttg
     1081 ttaattcatt cactagaaaa ttatgataac atcgtaaaat cattgtatat cattgcgctg
     1141 gcatttggag tgattgcaac aattataact gccacaatca gttatgtatt ttcaacacaa
     1201 attactaaac cgcttgtcag tttatcaaat aaaatgattg agattcgacg agatggtttt
     1261 caaaataaat tgcaattaaa tacaaattat gaagaaatag ataatttagc aaatacgttt
     1321 aatgagatga tgagccaaat tgaagaatca tttaatcaac aaagacaatt tgttgaagat
     1381 gcgtcacatg aattacgaac accattacaa attattcaag gtcatttaaa tttgattcag
     1441 cgatggggaa aaaaagaccc agcagtatta gaagaatcgt taaatatttc tattgaagaa
     1501 atgaatcgta tcataaaatt agtcgaagaa ttacttgaat tgactaaagg agatgtaaat
     1561 gacatttctt ctgaagcaca gaccgtgcat attaatgatg aaattcgctc gcgaatacac
     1621 tcattaaaac aattgcatcc tgattatcaa tttgatacgg atctgacatc taaaaatcta
     1681 gaaattaaaa tgaaacctca tcaattcgaa caattatttt taatctttat tgataatgca
     1741 atcaaatatg atgtgaagaa taagaaaatt aaagttaaga caaggttaaa aaataagcaa
     1801 aaaataattg aaattacaga tcatggaatt ggtattccag aggaagatca agatttcatt
     1861 tttgatcgct tttatcgagt ggataaatct cgttcaagaa gtcaaggcgg taatggactc
     1921 ggattatcta ttgctcaaaa aatcattcaa ttaaacggag gatcgattaa aattaaaagt
     1981 gaaattaaca aaggaacaac gtttaaaatc atattttaat catgactgag acgtcaatca
     2041 aagtcatagg atcaattttt taagtacaca ttagctgtga ctaatgtata agaacaacta
     2101 taaaacaaat aaacagtggt tctttatcat ttctgttgta ctcccaaaat ttacaataaa
     2161 atacatctat aaacctagaa gaatcaacgc ttttgttgat tcttcttttt agcagataaa
     2221 taggtaaatc tactttaaca aataactaaa tagtgatatt attacattgt aagcgtttca
     2281 acatttttgt ggagggtgta aaatgactaa cgaaagaaaa gaagtttcag aggctcctgt
     2341 aaacttcggt gcgaatttag gtctaatgtt agatctatat gatgactttt tacaagatcc
     2401 atcatctgta ccagaagatt tacaagtctt attcagcaca attaagaatg atgactcaat
     2461 tgtaccagct ttaaaaagta caagtagtca aaatagcgac ggcacaatta agcgtgtcat
     2521 gcgtttaatt gataatattc gccaatacgg gcatcttaaa gccgatattt atcctgtaaa
     2581 tcctccaaaa aggaaacatg tacctaaatt agagattgaa gactttgatt tagatcaaca
     2641 gactttggaa ggtatatcag caggaattgt ttcagatcac tttgccgaca tttatgataa
     2701 tgcttatgaa gcaattttaa gaatggaaaa acgttacaaa ggaccaattg catttgagta
     2761 tacacatatt aataacaata ccgaacgtgg ttggttaaaa agaagaattg aaacgccata
     2821 taaagtaacg ttaaataata acgaaaaaag ggcactattc aaacaattag cgtatgttga
     2881 agggtttgaa aaatatcttc ataaaaactt cgttggtgca aagcgttttt caattgaagg
     2941 ggtagacgca cttgtaccga tgttacaacg tactattacg attgctgcga aagaaggtat
     3001 taaaaatata caaataggca tggctcaccg tggacgttta aacgttttaa cgcatgtctt
     3061 agaaaaaccg tacgaaatga tgatttcaga atttatgcat acagatccaa tgaaattctt
     3121 acctgaagat ggtagcttgc agttaactgc tggatggact ggtgatgtga aatatcacct
     3181 tggtggcatt aaaactactg attcatacgg tacaatgcag cgtattgcac tggctaacaa
     3241 tccaagtcac ttggaaattg ttgcacctgt tgttgagggg cgtacgagag cagcacaaga
     3301 tgatacacaa cgagctgggg ctccgacgac tgatcatcat aaagcaatgc caattattat
     3361 acatggcgat gctgcttatc ctggtcaagg aattaacttc gaaacaatga acttaggaaa
     3421 cttgaaaggc tattctacgg gtggttcatt gcatattatt actaacaata gaattggatt
     3481 tactacagaa ccaattgatg cacgttcaac aacttattct acagatgtgg ccaaaggtta
     3541 tgatgtgcca atattccatg tcaatgcaga tgacgttgaa gctactattg aagcaattga
     3601 tattgcaatg gaatttagaa aagagtttca taaagacgtc gttattgatt tagtaggtta
     3661 tcgtcgtttc ggacataacg aaatggatga accatcaatt actaatccag ttccttatca
     3721 gaatattcgc aaacatgact ctgttgaata tgtgtttggt aaaaagcttg ttaatgaagg
     3781 tgtcatttca gaagatgaaa tgcattcatt tatagaacaa gtccaaaagg aactaagaca
     3841 agctcatgat aaaattaata aagctgataa aatggataat ccagatatgg aaaagcctgc
     3901 agatcttgca ttaccgttac aagcagacga acaatcattt acttttgatc acttgaaaga
     3961 aataaatgat gcattgttaa catatccgga tggctttaac attttgaaaa agttaaacaa
     4021 agttcttgag aagcgtcatg agccgtttaa taaagaagat ggtttagttg attgggcaca
     4081 agcagaacaa cttgcatttg cgacaatttt acaagatggt acaccgattc gcttaactgg
     4141 tcaagatagt gaacgtggta cattcagtca taggcatgcc gtgttacatg atgagcaaac
     4201 aggtgaaaca tatacacctt tacatcatgt tcctgatcaa aaagcgacat ttgatataca
     4261 caattctccg ctttcagaag cagcagtagt tggttttgaa tacggctata atgtggaaaa
     4321 caaaaaaagc ttcaatattt gggaagcaca atatggtgat tttgcaaata tgtcacaaat
     4381 gatttttgac aacttcttat tcagttctcg ctcaaaatgg ggagaacgtt caggattaac
     4441 attattctta cctcatgcat atgagggtca agggcctgaa cattcatcag caagattaga
     4501 gcgattttta caattagctg ctgaaaataa ttgcacagtt gtcaacttat ctagttcaag
     4561 taattatttc cacttattgc gtgcacaagc ggctagttta gattctgaac aaatgcgacc
     4621 attggttgtt atgtcaccaa aaagcttact gagaaataaa acagttgcaa aaccaattga
     4681 tgaatttact tctggtggat ttgagccaat tttgacagaa tcatatcaag cggataaggt
     4741 tacaaaagtt attttggcaa ctggtaaaat gttcattgat ttaaaagaag cattagctaa
     4801 aaatccagac gaatcagtat tactcgttgc gattgaaaga ttgtatccat tcccagagga
     4861 agagattgaa gcattactag cacaattgcc aaaccttgaa gaagtgtcat gggtacaaga
     4921 agaacctaaa aatcaaggtg catggttata tgtctatcca tatgttaaag tgctagttgc
     4981 agataaatat gatttaagtt attatggcag aattcaaagg gctgctccag ctgaaggcga
     5041 tggagaaatt cataaacttg ttcaaaataa aattatagaa aatgcattaa aaaataacta
     5101 gggggaaata agtcatgcca gaggttaaag ttccagaatt agcagaatct attacagaag
     5161 gtaccattgc agaatggttg aaaaacgtag gggatagcgt agaaaaaggt gaagctattc
     5221 ttgaattaga aactgataaa gttaatgtcg aagttgtatc tgaagaagca ggtgtattat
     5281 ctgaacaact tgcaagtgaa ggcgacactg tagaagttgg acaagcaatt gctatcatcg
     5341 gcgaaggtag tggcaatgct tctaaagaaa atagtaacga caatactcca caacaaaatg
     5401 aagaaacaaa taataaaaaa gaagaaataa caaataattc ggtagataaa gctgaagtaa
     5461 atcaagcaaa tgatgacaat cagcaacgta ttaatgctac gccttctgcg cgtcgatatg
     5521 ctcgtgaaaa tggtgtgaat cttgctgaag taagtccgaa aacaaatgat gtggttcgta
     5581 aagaagatat tgataagaaa caacaggcac cggcatcaac acaaacaaca caacaagcat
     5641 ctgcaaaaga agagaaaaaa tacaatcaat atcctacaaa accagtgatt cgtgaaaaaa
     5701 tgtcacgtag aaagaaaaca gctgccaaaa aattattaga ggtatctaat aatacagcta
     5761 tgttaacaac atttaacgaa gttgacatga caaatgttat ggaattgcgt aaacgtaaga
     5821 aagaacaatt tatgaaagat catgatggta ctaaattagg atttatgtca ttctttacta
     5881 aagcttctgt agcagctttg aaaaagtatc cagaagttaa tgcagaaatc gacggcgacg
     5941 acatgattac gaaacaatat tatgatattg gtgtagctgt ttctacagat gatggattat
     6001 tagtaccatt tgtaagagat tgtgataaaa agaattttgc agaaatcgaa gcagaaattg
     6061 ctaatttagc agttaaagca cgagagaaaa aacttggctt agatgatatg gttaatggtt
     6121 catttacgat tacaaatggc ggtatttttg gatcaatgat gagtacgcca attatcaatg
     6181 gtaatcaagc tgcaatctta ggcatgcatt caattattac aagaccaatt gcgattgatc
     6241 aagatacaat cgaaaatcgt ccaatgatgt atattgcatt aagctatgat catagaatta
     6301 ttgacggtaa agaagcagtt ggattcttaa aaacaattaa agaattaatt gaaaacccag
     6361 aagacttatt attagaatct taatccacaa cacaaaatag tttactattc ctaaaagcgg
     6421 gattaaatca ataacaaaca gcagtaagat tatttcctag tcgaaatatc ttactgctgt
     6481 actttatttt tataatgatt cgtattaaaa tgtgggttta attttgacta cttaaaaaat
     6541 atatcatttc tattgaaata gactcacaat agaaatatag gtagaatagc gtgtttcaat
     6601 atgctaatga atgcatttta gataataaca aacgatgaaa tatatgaatt ctataagcgc
     6661 ctttagttaa tttaaaatca tctgaacatg atgtaaattc gctttatgga cacgacatat
     6721 atatgttttt catgtcctac aaaaaacgaa atattcaatt gctatataca gatattcatt
     6781 aatgacacac tcaatagcaa ccataacaat attatgttac ctatttaaat gaaatagttc
     6841 ttttaaagaa atacattttt cacatattaa tctataatca aaatcaactg ac
//
LOCUS       DAXWFK010000133         6724 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.133, whole genome shotgun sequence.
ACCESSION   DAXWFK010000133 DAXWFK010000000
VERSION     DAXWFK010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6724
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.133"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            41..1234
                     /gene="fmtA"
                     /locus_tag="ACGEXS_002069"
     CDS             41..1234
                     /gene="fmtA"
                     /locus_tag="ACGEXS_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala esterase FmtA"
                     /protein_id="HFF8013062.1"
                     /translation="MKFNKVKLVIHACVLLFIIISIALIFHRLQTKTHSIDPIHKETK
                     LSDNEKYLVDRNKEKVAPSKLKEVYNSKDPKYKKIDKYLQSSLFNGSVAIYENGKLKM
                     SKGYGYQDFEKGIKNTPNTMFLIGSAQKFSTGLLLKQLEEEHKININDPVSKYLPWFK
                     TSKPIPLKDLMLHQSGLYKYKSSKDYKNLDQAVKAIQKRGIDPKKYKKHMYNDGNYLV
                     LAKVIEEVTGKSYAENYYTKIGDPLKLQHTAFYDEQPFKKYLAKGYAYNSTGLSFLRP
                     NILDQYYGAGNLYMTPTDMGKLITQIQQYKLFSPKITNPLLHEFGTKKYPDEYRYGFY
                     AKPTLNRLNGGFFGQVFTVYYNDKYVVVLALNVKGNNEVRIKHIYNDILKQNKPYNTK
                     GVIVQ"
     gene            complement(1771..2049)
                     /gene="qoxD"
                     /locus_tag="ACGEXS_002070"
     CDS             complement(1771..2049)
                     /gene="qoxD"
                     /locus_tag="ACGEXS_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit IV"
                     /protein_id="HFF8013063.1"
                     /translation="MKHTVGFIASIVLTLLAVYVTLYTSLTFHAKLTIIFGFAFVQAG
                     LQLLMFMHLTEGKDGRLQTFKVIFALVITLCFVVGTYWVMQGGHSSHL"
     gene            complement(2058..2663)
                     /gene="qoxC"
                     /locus_tag="ACGEXS_002071"
     CDS             complement(2058..2663)
                     /gene="qoxC"
                     /locus_tag="ACGEXS_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit III"
                     /protein_id="HFF8013064.1"
                     /translation="MSHDTNTIDSRTHEGELNKLGFWIFITAEFALFGTLFATLLTLQ
                     HGGDYAGKMTTELFELPLVLIMTFALLFSSYTCGIAIYYMRQEKQKLMMFWMIITLLL
                     GLVFVGFEIYEFAHYASEGVNPTIGSYWSSFFILLGTHGCHVSLGIVWAICLLIQIQR
                     RGLDKYNAPKLFIVSLYWHFLDVVWVFIFTAVYMIGMVYSG"
     gene            complement(2653..4641)
                     /gene="qoxB"
                     /locus_tag="ACGEXS_002072"
     CDS             complement(2653..4641)
                     /gene="qoxB"
                     /locus_tag="ACGEXS_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit I"
                     /protein_id="HFF8013065.1"
                     /translation="MNFPWDQLLVKGNWMITMAQIGAPFLVIGLIAVITYFKLWKYLY
                     KEWFTSVDHKKIGIMYLICAVLMFVRGGIDALLIRAQLTVPDNKFLESNHYNEIFSTH
                     GVIMIIFMAMPFIFGLWNIVVPLQIGARDVAFPVLNNVSFWLFFAGMILFNLSFIIGG
                     SPAAGWTNYAPLAGEFSPGPGVNYYLIAIQISGLGTLATGINFFVTILRCKTPTMKFM
                     QMPMFTVTTFITTLIVILAFPPLTVALALMTTDRIFDTAFFTVAHGGMPMLWANFFWV
                     WGHPEVYIVILPAFGIYSEIIPTFARKRLFGHQSMVWATAGIAFLSFLVWVHHFFTMG
                     NGALINSFFSISTMLIGIPTGVKLFNWLLTLYKGRITFESPMLFSLAFIPNFLLGGVT
                     GVMLAMASADYQYHNTYFLVAHFHYTLVTGVVFACLAGLIFWYPKMMGYKLNETLNKW
                     CFWFFMIGFNVCFLPQFILGLDGMPRRLYTYMPSDGWFLLNLISTIGALLMAIGFLFL
                     VVSIVYSHFKSPREATGDNWDGLGRTLEWTTASAIPPKYNFAITPDWNDYDTFVDMKE
                     HGRHYLDNHNYKDIHMPNNTPVGFWIGIFMTIGGFFLIFETVIPALICLFGIFGTMIY
                     RSFQIDHGYHIPAAEVAETEARLREARIKEREAVSHES"
     gene            complement(4641..5741)
                     /gene="qoxA"
                     /locus_tag="ACGEXS_002073"
     CDS             complement(4641..5741)
                     /gene="qoxA"
                     /locus_tag="ACGEXS_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit II"
                     /protein_id="HFF8013066.1"
                     /translation="MSKFKSLLLLFGTLILLSGCSNIEIFNAKGPVASSQKFLILYSI
                     VFMLVICFVVLGMFAIFIYKYSYNKNAESGKMHHNAIIETIWFVIPIIIVAALAIPTV
                     KTLYDYEKPPKSEKDPMVVYAVSAGYKWFFAYPDEHIETVNTLTIPKDRPVVFKLQAM
                     DTMTSFWIPQLGGQKYAMTGMTMNWTLEASQTGTFRGRNSNFNGEGFSRQTFKVNAVS
                     QKDYDKWVKEVKGKKTLDQDTFDKQLLPSTPNKALEFNGTHMAFVDPAADPEYIFYAY
                     KRFNFELKDPNFTSEENMFKDVSDKPLIPARKAQITNANYKRHGMKLMILGNDEPYNN
                     EFKKDESKNAKEMKKISKDAQDQDNDDHGGGH"
     gene            6043..6165
                     /locus_tag="ACGEXS_002074"
     CDS             6043..6165
                     /locus_tag="ACGEXS_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013067.1"
                     /translation="MIERKRKHFNKEFAKSNLSLNNEKWKFVCSKSKHFFKKSS"
     gene            complement(6313..6630)
                     /locus_tag="ACGEXS_002075"
     CDS             complement(6313..6630)
                     /locus_tag="ACGEXS_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HFF8013068.1"
                     /translation="MNKLLQSLSALGVSATLVTPNLNADATTNTTPQIKGANDIVIKK
                     GQDYNLLNGISAFDKEDGDLTDKIKVDGQIDTSKSGKYQIKYHVTDSDGAIKISTRYI
                     EVK"
BASE COUNT         2394 a         1060 c         1143 g         2127 t
ORIGIN      
        1 ttaaatatat aaaatgaata cattgtgagg caattatcaa atgaaattta ataaagtaaa
       61 actagttata catgcgtgtg tactattatt tatcattatt tctatagcgt taatttttca
      121 tcgattacag acgaagacac attctataga cccaatacat aaggaaacaa aattatcaga
      181 caatgaaaaa tatttagtgg atcgtaataa ggaaaaggtt gcgccgtcta aactaaaaga
      241 ggtatataat agcaaggatc ctaaatataa gaaaattgac aagtatttac aaagttcatt
      301 atttaacggt tcagtagcta tatatgaaaa tggcaaattg aaaatgagta aaggttatgg
      361 atatcaagat tttgaaaaag gtattaaaaa cacaccgaat acgatgtttt taataggttc
      421 agctcaaaaa ttttcaacag ggttactgtt aaaacagtta gaagaagaac ataaaataaa
      481 tatcaatgat ccagtaagta aataccttcc atggtttaaa acatctaagc ctatcccatt
      541 gaaagattta atgttgcatc aaagtggatt atataaatat aaatcctcaa aagattataa
      601 aaatttagat caagcagtta aagcgattca aaaacgtggt attgatccta agaaatacaa
      661 aaagcatatg tataacgatg ggaattattt agtacttgcg aaagtaattg aagaagttac
      721 aggtaaatct tatgctgaga attattatac aaaaatagga gatcctttaa aacttcagca
      781 cacagcattt tatgatgaac aaccttttaa aaaatatcta gcaaaaggtt atgcttataa
      841 tagtacagga ctttcattct taagacctaa tattttggac caatactatg gtgcaggtaa
      901 tttatatatg acaccaacag atatgggtaa attaattact caaatacaac aatataaatt
      961 attcagtcct aaaataacca atccattatt acatgagttt ggtacgaaaa agtatccaga
     1021 tgaatatcga tatggtttct atgctaagcc aacattgaat agacttaacg ggggattctt
     1081 tggacaagtc tttactgttt actataatga taagtatgta gttgtacttg cattaaatgt
     1141 aaaaggaaac aatgaagttc gaatcaaaca tatttataat gatattttaa aacaaaataa
     1201 accttacaat acgaagggtg ttattgttca ataattaata tagaagatat aacatgtata
     1261 tggcattaag gcatcgacct tatctgacca gtatacgagt tatatcttct tttttatagt
     1321 ggtaaaaagt ttaaagtata aggttgaaga aggatgagtt taaaaatatg tgttaactga
     1381 taaaagggga aatcatttgg tgagttggca tcagactaaa atgaatgaag acgaatacgt
     1441 tggtccatgc gtggtgtgaa tgttttctaa atctaactgg aaatgataat ttaataatgt
     1501 acacttttga ttgtttaaac atgtacaatt tttaaaaacc tagggataat tttattgacg
     1561 tgaacaacat tgagattatc tttagacata aatatggatt ttgaagttac tatatttatt
     1621 agttgtggtt gttaatgtat taattgaatt gattataatg attgtcttat aagatataga
     1681 aggggactta accatttaaa aattgaagga aaataaaaac cagctaacaa aagtgttagc
     1741 tggttaaact ttacattatt aaaatgttgt ttataagtgt gaagagtgac cgccttgcat
     1801 aacccaatat gttccgacaa cgaaacaaag tgtaattaca agagcaaaga taactttgaa
     1861 tgtttgtaaa cgtccatctt taccttcagt taaatgcatg aacattaata attgaagtcc
     1921 tgcttggacg aatgcaaagc caaagataat tgtcaacttc gcgtggaatg ttaatgacgt
     1981 gtatagtgtt acgtaaactg ctaaaagcgt taatacgata gatgcgataa atcctacagt
     2041 atgtttcatt attgtactca tccgctatac accatcccta tcatatatac ggcagtaaag
     2101 atgaaaaccc aaacaacatc taagaagtgc cagtataaac ttactataaa taattttggc
     2161 gcattatatt tgtctaatcc gcgtcgttgg atttggatta ataaacaaat ggcccaaacg
     2221 atacctagcg atacgtgaca accatgcgtt cctaatagga taaagaaact agaccagtaa
     2281 gaaccaattg ttgggttaac gccttctgat gcatagtgtg cgaattcata aatttcgaat
     2341 ccaacaaaga ctaaacctaa aagtaacgta atgatcatcc aaaacatcat taacttttgt
     2401 ttttcttggc gcatgtagta aatagcaata ccacatgtgt aagaactgaa taataatgca
     2461 aacgtcatta ttaaaacaag aggcaattca aataactcag tagtcatttt acctgcataa
     2521 tcgccaccat gttgcaaagt taatagtgtc gcaaataggg taccgaataa cgcaaattcg
     2581 gctgtaatga aaatccaaaa gccaagctta tttaattcgc cttcatgtgt gcgtgaatca
     2641 atagtgtttg tatcatgact catgacttac agcctccctt tctttaatac gcgcttctct
     2701 taatctagct tcagtttctg caacttcagc agcagggata tgatatccgt gatcgatttg
     2761 gaaactgcga taaatcatag taccaaaaat accgaataaa caaattaatg ctggaattac
     2821 agtttcgaaa attaagaaga aaccgccgat agtcataaag ataccaatcc agaatccaac
     2881 aggagtattg tttggcatat gaatgtcttt gtaattatgg ttgtctaagt aatgacgacc
     2941 atgttctttc atatcaacaa atgtgtcgta gtcattccaa tctggtgtaa tggcaaagtt
     3001 gtatttaggt ggaattgctg atgcagtagt ccactctaga gtacgaccaa ggccatccca
     3061 gttatctcca gttgcttcac gtggagattt gaagtgactg tatacgatac taacaacaag
     3121 gaataagaat ccgattgcca ttaataatgc accgatagtt gaaattaagt ttaataagaa
     3181 ccaaccatct gatggcatat aagtgtataa acgacgtggc ataccatcta atccaagaat
     3241 gaattgtggt aagaaacaaa cgttaaatcc gatcatgaag aaccagaagc accatttgtt
     3301 taatgtttcg tttaatttgt aacccatcat ctttggatac cagaagatta aaccagctaa
     3361 gcaggcaaat acaacaccag taaccaatgt atagtggaag tgagctacta agaagtacgt
     3421 attgtgatat tgatagtcag ctgatgccat tgctaacatt acacccgtaa caccacctaa
     3481 aaggaagtta gggataaatg ctaatgagaa tagcattggt gactcaaatg taatacgtcc
     3541 tttatataat gttaataacc agttaaacaa tttcacacca gttggaatac caatcagcat
     3601 tgttgaaatt gagaagaatg agttgattaa cgcaccatta cccattgtga agaaatggtg
     3661 aacccaaact aagaaactaa ggaacgcgat accggcagtt gcccatacca tactttgatg
     3721 tccgaataaa cgcttacgag cgaatgtcgg gataatttct gagtaaatac caaatgctgg
     3781 aaggataacg atataaactt cagggtgccc ccatacccag aagaagttag cccaaagcat
     3841 tggcataccg ccatgtgcaa ctgtgaagaa tgctgtgtca aatattctat cagttgtcat
     3901 taatgctaac gctactgtta aaggagggaa agcaagaata acaattaatg tagtaataaa
     3961 tgttgttact gtaaacattg gcatttgcat aaacttcata gttggtgttt tacatcttaa
     4021 aattgttaca aagaagttga tacctgtagc taaggtacca agccctgaaa tttgtatagc
     4081 tattaagtaa tagttaacac ccggaccagg actgaattca cctgctagtg gcgcatagtt
     4141 tgtccaacca gctgctggtg aaccaccaat aataaatgac aggttgaata aaatcatacc
     4201 tgcaaagaat agccagaaac ttacgttgtt taatactggg aatgcaacat cacgtgctcc
     4261 aatttgtaat ggaacaacga tattccataa accaaagata aatggcattg ccatgaagat
     4321 aatcatgatt acaccatgtg tactaaaaat ttcgttatag tggttagatt ctaaaaattt
     4381 gttatcaggt actgttaatt gcgcacgaat aagtaacgca tcaataccac cacggacgaa
     4441 cattaatacg gcacagatta aatacataat accgattttc ttatggtcta cagatgtgaa
     4501 ccattctttg taaagatatt tccataattt aaagtaagta attactgcga ttaaaccaat
     4561 aactaagaat ggggcaccaa tttgtgccat tgtaatcatc cagttacctt taactagtaa
     4621 ttgatcccat ggaaaattca ttaatgtcca cctccatgat catcattgtc ttgatcttgc
     4681 gcatcttttg aaattttctt catttctttc gcatttttcg attcatcttt cttgaactca
     4741 ttgttatatg gttcgtcatt tccaagaatc atcaacttca taccatgtcg tttatagttc
     4801 gcatttgtaa tttgagcttt acgagcaggt attaatggtt tgtctgatac atctttaaac
     4861 atattttctt cactagtgaa gtttggatct ttcaattcga aattgaaacg tttatatgca
     4921 tagaagatgt attctggatc ggctgctgga tcaacaaacg ccatatgtgt accattaaat
     4981 tctaaagctt tattaggtgt gcttggtaat aattgtttat caaatgtatc ttgatctaac
     5041 gttttcttac ctttaacttc tttcacccat ttgtcgtagt ctttttgact aacggcattt
     5101 actttaaatg tttgacgtga gaatccttca ccattgaagt tagagttacg acctctgaac
     5161 gtaccagttt gagatgcttc taacgtccaa ttcattgtca tgccagtcat ggcatatttt
     5221 tgaccaccta attgtggaat ccagaaactt gtcattgtat ccatagcttg aagcttaaat
     5281 acaacaggac gatctttagg gattgttaat gtattaacag tctctatatg ttcatctgga
     5341 taagcaaaga accatttgta tcctgcactt actgcatata caaccattgg atctttctca
     5401 ctcttcggtg gtttttcgta atcgtataaa gttttaactg taggaatagc taaagcagca
     5461 acgattatga taggtattac aaaccatatt gtttcaatga tggcattatg gtgcatctta
     5521 ccagattcgg cattcttatt ataactatac ttgtaaataa aaatggcgaa catgccaagt
     5581 acaacgaaac aaataacaag catgaagacg attgaataaa gaatcaagaa cttctgacta
     5641 cttgctactg gcccttttgc gttgaaaatt tctatatttg aacaaccact aagtaaaatt
     5701 agtgtgccaa ataatagaag caaagactta aattttgaca cttttttgac ctcctaatac
     5761 tacaaatgta gggcttaaca ttaattttaa gttattacac aatatttaca agggcttatg
     5821 ggaaaaaaat taataaaatt gtatcaaaaa tgttgataaa tcaaggtgtg acgtgggttc
     5881 acacatttgt taaaattatg tgtacatttt gtgactaata gcgtttttga aaaaattgct
     5941 tcatatttaa aaggaaagtt gagaatagct ttcaattaaa ggttaattga atgaaaataa
     6001 aaaaagtttg aaattgtcta ataaattgaa aatgatggct agttgattga aaggaagcgc
     6061 aaacatttta ataaggaatt tgccaaaagt aatttatcgt taaacaatga aaagtggaaa
     6121 tttgtttgtt caaaatcaaa acattttttt aaaaagagca gttaagtttt aataatttga
     6181 taggtagtcc tttggtatac agaaaaaata caatatttgg gttaaagttg cagatgaagt
     6241 tagaataaca aataaaatgg tagcaaaaca tgttcgtttg ctaccatttt atttgcagta
     6301 tagtgatgag ggctatttaa cctcaatata cctagtggaa attttaattg caccatctga
     6361 atcagtgaca tgatatttaa tttgatattt accagattta gatgtatcaa tttggccatc
     6421 gactttaatt ttatcggtta aatctccatc ttctttatca aatgcactta tgccgtttag
     6481 aaggttataa tcttgacctt tcttaataac gatatcatta gcgcctttaa tttgtggtgt
     6541 agtattcgtc gttgcatctg catttaaatt tggtgttact agtgtagcag aaacaccgag
     6601 ggctgataat gactgtaata gtttattcat aatgtaacct ccgaatgata agtcatttct
     6661 gagttagctt tactatacct taatttatat tatttacaca tcatactata gtcctaatct
     6721 tacc
//
LOCUS       DAXWFK010000134         6663 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.135, whole genome shotgun sequence.
ACCESSION   DAXWFK010000134 DAXWFK010000000
VERSION     DAXWFK010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6663
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.135"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            3..3506
                     /gene="sdrE"
                     /locus_tag="ACGEXS_002076"
     CDS             3..3506
                     /gene="sdrE"
                     /locus_tag="ACGEXS_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin SdrE"
                     /protein_id="HFF8013069.1"
                     /translation="MNRDNKKAITKKGMISNRLNKFSIRKYTVGTASILVGTTLIFGL
                     GNQEAKAAENTSTENAKQDDATTSDNKEVVSETENNSTTENNSTNPIKKETNTDSQPE
                     AKKESTSSSTQKQQNNVTATTETKPQNIEKENVKPSTDKTATEDTSVILEEKKAPNNT
                     NNDVTTKPSTSEPSTSEIQTKPTTPQESTNIENSQPQPTPSKVDNQVTDATNPKEPVN
                     VSKEELKNNPEKLKELVRNDSNTDHSTKPVATAPTSVAPKRVNAKMRFAVAQPAAVAS
                     NNVNDLIKVTKQTIKVGDGKDNVAAAHDGKDIEYDTEFTIDNKVKKGDTMTINYDKNV
                     IPSDLTDKNDPIDITDPSGEVIAKGTFDKATKQITYTFTDYVDKYEDIKSRLTLYSYI
                     DKKTVPNETSLNLTFATAGKETSQNVTVDYQDPMVHGDSNIQSIFTKLDEDKQTIEQQ
                     IYVNPLKKSATNTKVDIAGSQVDDYGNIKLGNGSTIIDQNTEIKVYKVNSDQQLPQSN
                     RIYDFSQYEDVTSQFDNKKSFSNNVATLDFGDINSAYIIKVVSKYTPTSDGELDIAQG
                     TSMRTTDKYGYYNYAGYSNFIVTSNDTGGGDGTVKPEEKLYKIGDYVWEDVDKDGVQG
                     TDSKEKPMANVLVTLTYPDGTTKSVRTDANGHYEFGGLKDGETYTVKFETPTGYLPTK
                     VNGTTDGEKDSNGSSVTVKINGKDDMSLDTGFYKEPKYNLGDYVWEDTNKDGIQDANE
                     PGIKDVKVTLKDSTGKVIGTTTTDASGKYKFTDLDNGNYTVEFETPAGYTPTVKNTTA
                     DDKDSNGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQDSTEKGIKDV
                     TVTLQNEKGEVIGTTKTDENGKYRFDNLDSGKYKVIFEKPAGLTQTVTNTTEDDKDAD
                     GGEVDVTITDHDDFTLDNGYFEEDTSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDAGKHTPVKPMSTTKDHHNKAKALPETGSENNGSNNATLF
                     GGLFAALGSLLLFGRRKKQNK"
     gene            3625..5097
                     /locus_tag="ACGEXS_002077"
     CDS             3625..5097
                     /locus_tag="ACGEXS_002077"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFF8013070.1"
                     /translation="MNYILGTILESKITGVEKAQINRLKLFKQHGISSKCVYVKWNPY
                     SYTYAKQHQIENDVFTMYDYFQKAINYKKTKQVNWIQYWEKSCRYTLKFVENSNDVRI
                     YDEEQFVMYAHFLDKQYHQLNYVNYFDHKRRKVKRELYDGRGFLSCSRILGEGQRIVL
                     ENYYTPNGEIVIQKYFDDIKGKNTLTKVILNEDQHQQFFDTEDELVQYFLHQLCKNND
                     QIILDRPHELGNVIAGLNQSIPVIVVLHSTHLSGAGNGIKSFYKTVFNNLTRYKAIVV
                     STEKQCQDISQYIENKIPVINIPVGYVANLKYQFDINQKEKNHIISIARLVENKQIKH
                     QIEVIKQLVTKHPNIQLNIYGHGNGLSEYRQLVEDYHLSEHVKFHGFKTHINEEIAKA
                     ELMLSTSKMEGFGLAILESLSVGTPVISYDVDYGPSELIQDGFNGYLVPQGDINQMVE
                     KVDQLLNNTQKLQQFSINSIESAQQYNATTISTKWQNILN"
     gene            complement(5221..>6663)
                     /locus_tag="ACGEXS_002078"
     CDS             complement(5221..>6663)
                     /locus_tag="ACGEXS_002078"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFF8013071.1"
                     /translation="EKAIINRLNLFKEMGRPAQCVFLSWNRYLYRNAQNYITSSDYIN
                     MYDFFQEATYLERNEPFDWLSYWTDECHYTLKHVENSHDFRIYDQERFLMYAHFQDPK
                     YRILDYVNHFDSQRRKVKRDFYDVRGFLSCSRILVDKQQTLCEFFYNPEGDTKLEKYF
                     SYKDGKPEVQKIIVYYANKQYFFNNETELGAFFIKQLYQHGDLFFSDRNVYTAPIFNL
                     TPESIPVVAVLHSTHIKNIDALDSSPFKNVYKAMFENLSRYRAIIVSTEQQKLDVEKR
                     INHTIPVVNIPVGYSETIDTPVQTLDQRSVKLISVARYSPEKQLHQQIELIKRLVSYV
                     PKIELHMYGFGSESKKLNELIQKYGLENHVYLRGFLSNLDQEYSDAYLSLITSNMEGF
                     SLALLESLAHGVPVISYDIKYGPNELITSDFNGYLITKNDEDALFDKVKYVIDHPEVQ
                     QRLSKGSLAKAQQYSKASLIKQWDQFVRLI"
BASE COUNT         2580 a         1053 c         1132 g         1898 t
ORIGIN      
        1 tgattaacag ggataataaa aaggcaataa caaaaaaggg tatgatttca aatcgcttaa
       61 acaaattttc gattagaaag tatactgtag gaactgcatc gattttagta ggtacgacat
      121 tgatttttgg tctagggaac caagaagcta aagctgctga aaacactagt acagaaaatg
      181 caaaacaaga tgatgcaacg actagtgata ataaagaagt agtgtcggaa actgaaaata
      241 attcgacaac agaaaataat tcaacaaatc caattaagaa agaaacaaat actgattcac
      301 aaccagaagc taaaaaagaa tcaacttcat caagtactca aaaacagcaa aataacgtta
      361 cagctacaac tgaaactaag cctcaaaaca ttgaaaaaga aaatgttaaa ccttcaactg
      421 ataaaactgc gacagaagat acatctgtta ttttagaaga gaagaaagca ccaaataata
      481 caaataacga tgtaactaca aaaccatcta caagtgaacc atctacaagt gaaattcaaa
      541 caaaaccaac tacacctcaa gaatctacaa atattgaaaa ttcacaaccg caaccaacgc
      601 cttcaaaagt agacaatcaa gttacagatg caactaatcc aaaagaacca gtaaatgtgt
      661 caaaagaaga acttaaaaat aatcctgaga aattaaaaga attggttaga aatgatagca
      721 atacagatca ttcaactaaa ccagttgcta cagctccaac aagtgttgca ccaaaacgtg
      781 taaacgcaaa aatgcgcttt gcagttgcac aaccagcagc agttgcttca aacaatgtaa
      841 atgatttaat taaagtgacg aagcaaacaa tcaaagttgg cgatggtaaa gataatgtgg
      901 cagcagcgca tgacggtaaa gatattgaat atgatacaga gtttacaatt gacaataaag
      961 tcaaaaaagg cgatacaatg acgattaatt atgataagaa tgtaattcct tcggatttaa
     1021 cagataaaaa tgatcctatc gatattactg atccatcagg agaggtcatt gctaaaggaa
     1081 catttgataa agcaactaag caaatcacat atacatttac agactatgta gataaatatg
     1141 aagatataaa atcacgctta actctatatt cgtatattga taaaaaaaca gttccaaatg
     1201 agacaagttt gaatttaaca tttgctacag caggtaaaga aacaagccaa aatgtcactg
     1261 ttgattatca agatccaatg gtccatggtg attcaaacat tcaatctatc tttacaaaat
     1321 tagatgaaga taagcaaact attgaacaac aaatttatgt taacccattg aaaaaatcag
     1381 caaccaacac taaagttgat atagctggta gtcaagtaga tgattatgga aatattaaac
     1441 taggaaatgg tagcaccatt attgaccaaa atacagaaat aaaggtttat aaagttaact
     1501 ctgatcaaca attgcctcaa agtaatagaa tctatgattt tagtcaatac gaagatgtaa
     1561 caagtcaatt tgataataaa aaatcattta gtaataatgt agcaacattg gattttggtg
     1621 atattaattc agcctatatt atcaaagttg ttagtaaata tacacctaca tcagatggcg
     1681 aactagatat tgcccaaggt actagtatga gaacaactga taaatatggt tattataatt
     1741 atgcaggata ttcaaacttc atcgtaactt ctaatgacac tggcggtggc gacggtactg
     1801 ttaaacctga agaaaagtta tacaaaattg gtgactatgt atgggaagac gttgataaag
     1861 acggtgttca aggtacagat tcaaaagaaa aaccaatggc aaacgtttta gttacattaa
     1921 cttacccgga cggtactaca aaatcagtaa gaacagatgc taatggtcat tatgaattcg
     1981 gtggtttgaa agacggagaa acttatacag ttaaattcga aacgccaact ggatatcttc
     2041 caacaaaagt aaatggaaca actgatggtg aaaaagactc aaatggtagt tcggttactg
     2101 ttaaaattaa tggtaaagat gatatgtctt tagatactgg tttttacaaa gaacctaaat
     2161 acaacttagg tgactatgta tgggaagata ctaataaaga tggtatccaa gatgcaaatg
     2221 agccaggaat caaagatgtt aaggttacat taaaagatag tactggaaaa gttattggta
     2281 caactactac tgatgcctcg ggtaaatata aatttacaga tttagataat ggtaactata
     2341 cagtagaatt tgaaacacca gcaggttaca cgccaacggt taaaaatact acagctgatg
     2401 ataaagattc taatggttta acaacaacag gtgtcattaa agatgcagat aatatgacat
     2461 tagacagtgg tttctataaa acaccaaaat acagtttagg tgattatgtt tggtacgaca
     2521 gtaataaaga cggcaaacaa gattcaactg aaaaaggtat caaagatgtg acagttacat
     2581 tgcaaaacga aaaaggcgaa gtaattggaa caactaaaac agatgaaaat ggtaaatatc
     2641 gtttcgataa tttagatagc ggtaaataca aagttatttt tgaaaagcct gctggcttaa
     2701 cacaaacagt tacaaataca actgaagatg ataaagatgc agatggtggc gaagttgacg
     2761 taacaattac ggatcatgat gatttcacac ttgataacgg atacttcgaa gaagatacat
     2821 cagacagcga ttcagactca gatagtgact cagacagcga ctcagactca gacagcgact
     2881 cagactcaga cagtgattca gattcagaca gcgactcaga ttcagatagc gactcagatt
     2941 cggacagcga ttcagactca gatagcgact cagattcaga tagcgattca gactcagaca
     3001 gcgactcaga ttcagatagc gattcggact cagacagcga ttcagactca gatagcgact
     3061 cagactcaga cagcgactca gattcagata gcgattcgga ctcagatagc gactcagatt
     3121 cagacagcga ttcagactca gatagcgact cagattcaga cagcgattca gactcagata
     3181 gcgactcaga ctcagacagt gattcagatt cagacagcga ctcagactca gatagcgact
     3241 cagattcgga cagcgactca gactctgata gcgactcaga ctcagacagt gattcagact
     3301 cagatagcga ttcggactcg gatgcaggaa aacatacacc tgttaaacca atgagtacta
     3361 ctaaagacca tcacaataaa gcaaaagcat taccagaaac aggtagtgaa aataacggct
     3421 caaataacgc aacgttattt ggtggattat ttgcagcatt aggttcatta ttgttattcg
     3481 gtcgtcgcaa aaaacaaaac aaataataca atatgaccca ggtccttgtg gcctggtttt
     3541 tttataatta cacatgcaat agatgtattt ttcatataaa ataaacaata aagatacgga
     3601 ataaaactta tatgaggcga taatatgaat tacattttag gaacaatttt agaaagtaaa
     3661 attacaggtg tagaaaaagc gcaaataaat agattgaagt tgttcaaaca acacggcata
     3721 tcttcaaaat gtgtatatgt taaatggaat ccttattcat acacatatgc gaagcaacat
     3781 cagattgaaa atgatgtatt tacaatgtat gactattttc aaaaagcaat caattataaa
     3841 aagacaaagc aagttaactg gatacagtat tgggaaaagt catgtaggta cacattgaaa
     3901 tttgtggaaa attcaaatga tgtcagaata tatgatgaag agcaatttgt aatgtatgct
     3961 cattttttag ataaacagta tcatcaatta aattatgtga attattttga tcataaaaga
     4021 agaaaagtaa aacgcgaatt gtatgatgga agaggctttt taagttgttc tcgaatttta
     4081 ggtgaaggac aacggattgt actcgaaaat tactatacac ctaatgggga aatcgtcatc
     4141 caaaaatatt ttgacgatat aaaagggaaa aacacgctca caaaggttat cttaaatgaa
     4201 gaccagcatc aacaattttt tgatacagaa gatgaattag ttcaatattt tctccatcaa
     4261 ttatgtaaaa ataatgatca aatcatatta gatcgtcctc atgaattagg aaatgttata
     4321 gcgggattaa atcaaagtat tccagttatt gttgtgctcc atagtacaca tttatccggt
     4381 gccggtaatg gtataaaaag tttttataaa acagtgttta ataatttaac acgttataaa
     4441 gcgattgttg tatcaacaga aaagcaatgc caagatattt cacaatatat tgaaaataaa
     4501 ataccagtta tcaatattcc ggttggctac gtggcaaatt taaagtatca atttgacatc
     4561 aatcaaaagg agaaaaatca tatcatatca attgctcgcc tcgttgaaaa taaacaaatt
     4621 aaacatcaaa ttgaagtaat caagcaatta gtaacaaaac atcccaatat tcaattgaat
     4681 atttatggac atggaaatgg tttgtcagaa tatcgacaac ttgtagaaga ttatcattta
     4741 tcggaacatg ttaaatttca tggttttaag acgcatatta atgaagagat tgctaaagca
     4801 gaactgatgt tatcgacaag taaaatggaa ggttttggct tagcaatttt agagtcgctt
     4861 tcagtaggta caccagtgat cagttatgat gtagattatg gtccatcaga actgattcaa
     4921 gatggattta atggctattt agtacctcaa ggtgacatca atcaaatggt tgaaaaggtc
     4981 gaccaattac taaataatac tcaaaaattg caacagtttt caattaatag catagaatct
     5041 gcacaacagt acaatgcaac tactatcagt acaaagtggc aaaatatttt aaactaagtc
     5101 aaaagaaaaa agcattttcc tagaatcatc tatttaacaa acctcgttca tacgaactta
     5161 catgtttagt taaatgacat aggaaaatgc tatttttgta aaaataaagg taaagtacat
     5221 ttatataaga cgaacaaatt ggtcccattg tttaattaac gacgctttac tatattgttg
     5281 tgcttttgcc aaactacctt ttgacagtcg ttgctgtact tcaggatgat caatcacata
     5341 ttttacttta tcaaataggg catcttcatc atttttagta attaaataac cattgaaatc
     5401 tgaagtaatc agttcgttag gtccatattt aatatcataa ctaataactg gaacaccatg
     5461 tgctaaagat tcaagtagcg ctaaagagaa accttccatg ttacttgtta ttaaactcaa
     5521 ataggcatcg ctatattctt ggtctagatt gcttaaaaag ccgcgtaagt aaacatgatt
     5581 ttccaatcca tatttttgta tcaattcatt taatttttta ctttcagaac caaaaccata
     5641 catatgaagc tctatttttg ggacatacga tactaagcgt ttaattaatt caatttgttg
     5701 atgtaattgt ttttcaggtg aataacgagc aacggaaatt aatttaacac tgcgctgatc
     5761 taatgtttgg actggtgtat caattgtttc actatagccg acaggaatat taacaactgg
     5821 aatagtatgg ttaatacgtt tttcaacatc taatttttgc tgctcagtag aaacgataat
     5881 tgcacgatat cgagataaat tttcaaacat cgctttatat acatttttaa atggcgatga
     5941 atctaatgca tcaatatttt taatgtgtgt actgtgaagc acagctacta ctgggattga
     6001 ctcaggcgtt aagttgaaaa taggtgctgt gtacacatta cgatcactga aaaataaatc
     6061 cccatgttga tatagttgtt taatgaaaaa tgcgcctaat tccgtttcat tattaaagaa
     6121 atattgtttg ttagcatagt aaacaataat tttttgtact tctggtttgc catccttgta
     6181 agaaaaatac ttttctaatt ttgtgtcacc ttctggatta tagaaaaatt cacataatgt
     6241 ttgttgttta tcaacaagaa tcctactaca acttaaaaag ccacgcacat cataaaaatc
     6301 acgttttact tttcgtcttt gactatcaaa atgatttaca taatctaata tacgatattt
     6361 aggatcttga aaatgggcat acattaagaa acgctcttga tcatatattc taaagtcatg
     6421 actattttca acatgtttta aagtataatg acattcatca gtccaatacg acaaccagtc
     6481 aaatggttca ttgcgttcta aatatgttgc ttcttggaag aaatcataca tattaatata
     6541 gtcagaacta gtaatataat tttgggcatt tctatataaa tatctattcc atgacagaaa
     6601 tacacattgc gctggtcttc ccatttcttt aaataaattt aaacgattaa taattgcttt
     6661 ctc
//
LOCUS       DAXWFK010000135         6622 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.136, whole genome shotgun sequence.
ACCESSION   DAXWFK010000135 DAXWFK010000000
VERSION     DAXWFK010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6622
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.136"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(110..1465)
                     /gene="brnQ"
                     /locus_tag="ACGEXS_002079"
     CDS             complement(110..1465)
                     /gene="brnQ"
                     /locus_tag="ACGEXS_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFF8013072.1"
                     /translation="MKKKLTFKENMFIGSMLFGLFFGAGNLIFPIHLGQAAGSNVFIA
                     NLGFLITAIGLPFLGIIAIGISKTSGLFEIASRVNKTYAYIFTIALYLVIGPFFALPR
                     LATTSFEIAFSPFLSPKQITLYLFIFSFVFFVIAWFFARKPSRILEYIGKFLNPVFLV
                     LLAIILLFAFIHPLGGISDAPISKQYQSHALFNGFLDGYNTLDALASLAFGIIIVATI
                     KKLGIENPTDIAKETIKSGTISIIMMGIIYTLLAIMGTLSIGHFKLSENGGIALAQIT
                     QYYLGNYGIVLLSLIVMVACLKTAIGLITAFSETFEHLFPKMNYLAIATVVSFISFLF
                     ANVGLTKIIMYSVPVLMFLYPLAIALIVLTLFSSKFHHSKLIYQCTIFFTMIAALVDG
                     LKASPEFISSTSFSQTLINFSQKYLPLSDIGMGWVVLSLIGFIIGFIIYKIKHRKIPQ
                     A"
     gene            complement(1741..2298)
                     /locus_tag="ACGEXS_002080"
     CDS             complement(1741..2298)
                     /locus_tag="ACGEXS_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HFF8013073.1"
                     /translation="MINFNKTALVLIDLQEGILKMDYAPYTAENVVQNANKLIDVFRK
                     NNGFIAFVRVNFYDGKDVLQPNAMISLPPKEGDDYSRFHHLLDKRDDDFVIDKRHFSA
                     FVGTDLDLQLRRRGIDTIVLGGVATHIGVDTTARDAYQLNYNQFFVTDMMSAQNETLH
                     QFPIDNVFPLMGQTITTNDFLNILN"
     gene            complement(2365..4005)
                     /locus_tag="ACGEXS_002081"
     CDS             complement(2365..4005)
                     /locus_tag="ACGEXS_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-keto acid decarboxylase family protein"
                     /protein_id="HFF8013074.1"
                     /translation="MKQRIGAYLIDAIHRAGVDKIFGVPGDFNLAFLDDIISNPNVDW
                     VGNTNELNASYAADGYARLNGLAALVTTFGVGELSAVNGIAGSYAERIPVIAITGAPT
                     RAVEQGGKYVHHSLGEGTFDDYRKMFAHITVAQGYITPENATTEIPRLINTAIAERRP
                     VHLHLPIDVAISEIEIPTPFEVTATKYTDASTYIELLATKLHQAKQPIIITGHEINSF
                     HLHQELEDFVNQTQIPVAQLSLGKGAFNEENPYYMGIYDGKIAEDKIRDYVDNSDLIL
                     NIGAKLTDSATAGFSYQFNIDDVVMLNHHNIKIDDVTNDEISLPSLLKQLSNISYTNN
                     ATFPAYHRPTSPDYTVGTEPLTQQTYFKMMQNFLKPNDVIIADQGTSFFGAYDLALYK
                     NNTFIGQPLWGSIGYTLPATLGSQLADKDRRNLLLIGDGSLQLTVQAISTMIRQHIKP
                     VLFVINNDGYTVERLIHGMYEPYNEIHMWDYKALPAVFGGKNVEIHDVESSKDLQDTF
                     NAINGHPDVMHFVEVKMAVEDAPKKLIDIAKAFSQQNK"
     gene            4199..4300
                     /locus_tag="ACGEXS_002082"
     CDS             4199..4300
                     /locus_tag="ACGEXS_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013075.1"
                     /translation="MMLQNTIAKKKQARHLMKVYAPVYSKHYLASNN"
     gene            complement(4279..6324)
                     /gene="ptsG"
                     /locus_tag="ACGEXS_002083"
     CDS             complement(4279..6324)
                     /gene="ptsG"
                     /locus_tag="ACGEXS_002083"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-specific PTS transporter subunit IIBC"
                     /protein_id="HFF8013076.1"
                     /translation="MRKKLFGQLQRIGKALMLPVAILPAAGLLLAIGTAMQGESLQHY
                     LPFIQNGGVQTVAKLMTGAGGIIFDNLPMIFALGVAIGLAGGDGVAAIAAFVGYIIMN
                     KTMGDFLQVTPKNIGDPASGYASILGIPTLQTGVFGGIIIGALAAWCYNKFYNINLPS
                     YLGFFAGKRFVPIMMATTSFILAFPMALIWPTIQSGLNAFSTGLLDSNTGVAVFLFGF
                     IKRLLIPFGLHHIFHAPFWFEFGSWKNAAGEIIHGDQRIFIEQIREGAHLTAGKFMQG
                     EFPVMMFGLPAAALAIYHTAKPENKKVVAGLMGSAALTSFLTGITEPLEFSFLFVAPL
                     LFFIHAVLDGLSFLTLYLLDLHLGYTFSGGFIDYFLLGILPNKTQWWLVIPVGLVYAV
                     IYYFVFRFLIVKLKYKTPGREDKQSQAATASATELPYAVLEAMGGKANIKHLDACITR
                     LRVEVNDKSKVDVPGLKDLGASGVLEVGNNMQAIFGPKSDQIKHEMQQIMNGQVVENP
                     TTMEDDKDETVVVAEDKSATSELSHIVHAPLTGEVTPLSEVPDQVFSEKMMGDGIAIK
                     PSQGEVRAPFNGKVQMIFPTKHAIGLVSDSGLELLIHIGLDTVKLNGEGFTLHVEEGQ
                     EVKQGDLLINFDLDYIRNHAKSDITPIIVTQGNITNLDFKQGEHGNISFGDQLFEAK"
BASE COUNT         2180 a         1146 c         1175 g         2121 t
ORIGIN      
        1 aatatttttt gtggataatc attgatatgt atgtatacga ttgcagttta atgtagtatg
       61 aataaaaaat gccacaagca tatgtttaac atatgactgt ggcatagtat tatgcttgtg
      121 gaattttacg atgcttaatt ttataaataa tgaagccgat aatgaaacca atcaaactga
      181 gaacaaccca gcccatacca atgtctgata atggtaaata tttttggctg aaattaatca
      241 aagtttgtga gaatgatgtg cttgaaatga actctggact agcttttaat ccatctacta
      301 atgcagcaat cattgtaaag aaaatggtac attgataaat aagttttgaa tgatggaatt
      361 tgctactaaa taatgttagt acaatcaagg caattgctaa tggatataag aacattaaca
      421 ctgggactga gtacataata atcttagtta aaccaacatt cgcgaataag aacgaaataa
      481 agcttacaac tgttgcaatc gctaggtaat tcattttagg gaaaaggtgt tcgaatgttt
      541 ctgaaaatgc cgtaatcaaa ccgatggctg tttttaaaca agcaaccata acgataagtg
      601 acaacaggac gataccgtag ttacctaagt agtattgagt aatttgcgct aaggcaatac
      661 caccattttc actaagtttg aaatgaccaa tacttaatgt acccatgatt gctagtaggg
      721 tataaatgat ccccatcata atgatactga tagtaccaga cttaattgtt tctttagcga
      781 tatcagttgg attttcgata cctaactttt taatcgttgc aacaatgata ataccaaatg
      841 ccaatgacgc tagcgcatct aaggtattgt atccatctaa aaagccgtta aataaggcat
      901 gtgattgata ttgtttacta ataggtgcat cagatatgcc acctaatgga tggataaaag
      961 caaataataa aataattgct aataatacta agaataccgg atttaaaaat ttaccgatat
     1021 attctaaaat tcttgatggc tttctcgcaa aaaaccatgc aatcacaaag aagacgaagc
     1081 taaaaataaa taaatataaa gtgatttgct ttggtgataa aaatggcgaa aatgcaattt
     1141 caaatgatgt cgttgccagt ctaggtaagg cgaaaaatgg tccgataact agatataagg
     1201 caatcgtgaa aatgtaagca tatgttttat taacacgcga tgcaatttca aataaaccag
     1261 atgtctttga aatgccaata gcaatgatac ctagaaatgg taagccaatt gctgtaatta
     1321 aaaatcctaa gttagcgata aaaacgttag aaccagcagc ttgacccaag tgtattggga
     1381 agataagatt gccggcacca aagaataaac caaataacat agaacctata aacatgtttt
     1441 ctttaaatgt tagtttcttc ttcatatatg tatttgttct cctttttaga acttataaaa
     1501 atctacttaa ttaagataat atcaaaaatt aaacataaag tgatacataa ttttgcaata
     1561 aatactaaat tttttgaaaa ttccggaata aatactgttt tttatgtagt aggggcgtaa
     1621 gttaagaacg atttaacaga tatagaatat gttaaatata gaaggttaga caacccgacg
     1681 tattaatact ttgtgaaata aagatccccc tcaacatgat cgaagggggg aagtatatgt
     1741 ttagttcaat atatttagaa agtcgtttgt agttattgtt tgtcccatca atgggaatac
     1801 attatctatt ggaaattgat gtagcgtttc gttttgtgca ctcatcatat ctgtaacaaa
     1861 aaactgattg tagtttaatt gataggcatc tcgcgctgtc gtatctacgc caatatgcgt
     1921 tgcgacacca ccaagaacaa tcgtatcaat tcctcgacgt cgcaattgta agtccaaatc
     1981 tgttcctaca aatgcactaa aatgtcgttt gtctatgaca aaatcgtcat ctctcttgtc
     2041 taataaatga tggaaacgac tgtagtcgtc gccttctttt ggtggtaatg agatcattgc
     2101 atttggttgc aatacatctt taccatcata gaaattcacg cgaacaaaag cgataaagcc
     2161 attgtttttt ctaaaaacat ctattaattt attagcgttt tgaacgacat tttcagctgt
     2221 atatggggca taatccattt taagaatacc ttcttgcagg tcgattaaca ctaaagcggt
     2281 tttattaaaa ttaatcatct tatatcagag tcctattcgt ttaataaagt attcgcttaa
     2341 acttataccc tgtatacgat gaaattattt attttgttgt gaaaaagctt tagcgatatc
     2401 gatgagtttc ttcggtgcgt cttcgacagc cattttgact tcgacaaaat gcatcacatc
     2461 gggatgacca ttaattgcat taaacgtgtc ttgtaaatct tttgatgatt caacgtcatg
     2521 aatttcaaca tttttaccac caaatacagc tggtaaagct ttataatccc acatgtgaat
     2581 ttcattataa ggttcataca tgccgtgaat aagtcgttct accgtatagc cgtcattatt
     2641 aatcacaaat aataccggtt taatatgctg tctaatcata gttgaaatag cttgaacagt
     2701 tagttgcaat gagccatcac caattaataa taagttacga cgatctttgt ctgctaattg
     2761 tgaacctaat gttgcaggta atgtatagcc gatagaaccc cataacggtt gccctataaa
     2821 agtattgttt ttgtataatg ctaaatcata agcaccaaag aatgatgtac cttgatcagc
     2881 aatgatgaca tcatttggtt ttaagaaatt ttgcatcatt ttaaaataag tttgttgtgt
     2941 taatggttct gtgccaacag tataatcggg tgatgttgga cgatgatacg cagggaacgt
     3001 tgcgttattc gtatatgaaa tattggataa ctgttttaac aatgatggta gagatatttc
     3061 atcatttgta acatcgtcaa ttttgatatt gtgatgattt aacataacga catcatcgat
     3121 attgaattgg tatgaaaaac ctgctgttgc tgaatctgtt aatttggctc caatatttaa
     3181 aattaaatcg ctgttgtcca cataatctcg tattttatct tcggcaattt tcccatcgta
     3241 aatacccata taatatggat tttcctcatt aaaagcacct tttcctaatg aaagttgtgc
     3301 tactggtatc tgtgtttgat ttacaaaatc ttctaattct tgatggaggt gaaaactgtt
     3361 aatttcatgt ccagtaatga tgataggctg cttcgcttga tgcagtttag ttgctaataa
     3421 ctctatatat gttgatgcat ccgtatattt agttgccgtc acttcaaatg gtgtcggtat
     3481 ctcaatttca gagattgcga catcgattgg taaatgtaaa tgaactgggc gtctttcggc
     3541 gattgctgta ttaattaaac gtggtatttc ggttgttgca ttttcaggtg tgatataacc
     3601 ttgtgcaacg gttatatgtg caaacatttt tcgatagtcg tcaaatgtac cttcaccaag
     3661 tgagtgatgt acatatttac cgccttgttc aacagcacgt gtcggcgcac ctgtaatcgc
     3721 aatgacaggt atgcgttcag catatgaacc tgcgataccg ttgacggcac ttaattcgcc
     3781 aacaccaaat gtagtaacta atgcagcgag tccattaaga cgggcataac cgtccgctgc
     3841 gtaacttgcg tttaattcat ttgtatttcc tacccaatct acattgggat tgctgataat
     3901 atcgtctaga aaagcgagat taaaatcacc aggaacacca aaaattttat cgacgcctgc
     3961 tcgatgaata gcgtcaatta agtaagctcc aatgcgttgt ttcataaaaa aagccacctt
     4021 ttctctatat ttacacttaa aatgatagcc tcgaatatag tgtaaggtca aggaagttga
     4081 ctttgttgtg aaaacgctaa caatagtgaa caattaatat tttttaggaa atgagtatga
     4141 aggggggctt taattgatga tcttaaaatt caaaatttaa ggggagagcg aactatcaat
     4201 gatgctgcaa aatacaatag caaagaaaaa acaggcgcgt caccttatga aggtatacgc
     4261 acctgtttat agtaagcatt atttagcttc aaataattga tcgccaaatg aaatgttgcc
     4321 atgttcacct tgtttaaaat caaggtttgt aatgtttcct tgtgtcacga taataggcgt
     4381 aatatcactc tttgcatgat tgcggatgta gtctaaatca aagttgatta ataaatcacc
     4441 ttgtttaact tcttgacctt cctcaacatg taaagtaaag ccttctccgt ttaatttaac
     4501 agtgtctaaa ccgatgtgga ttaatagttc taaaccacta tctgatacaa gaccaattgc
     4561 atgttttgtt gggaaaatca tttgtacttt accgttgaat ggtgcacgaa cttcaccttg
     4621 tgaaggtttg atagcgatac cgtcacccat cattttttcg ctgaacactt gatcaggcac
     4681 ttctgataat ggtgttactt caccagttaa tggtgcatgc acgatatggc tcaattcgct
     4741 tgttgcagat ttatcttctg caacaacaac agtttcgtct ttatcgtctt ccatagtagt
     4801 aggattttct actacttgac cattcataat ctgttgcatt tcatgtttga tttggtcaga
     4861 tttaggacca aaaattgctt gcatattatt gccgacttct aatacaccag atgcgcctaa
     4921 atctttcaaa ccaggaacat caactttaga tttgtcgtta acttcaacac gtagacgtgt
     4981 gatacaagcg tctaaatgtt taatgtttgc tttgccaccc atagcttcta atactgcata
     5041 tggtaattca gttgctgaag cagtagccgc ttgtgattgt ttatcttcac gacctggtgt
     5101 tttgtatttt aattttacaa ttaagaatcg gaatacgaag tagtaaataa ctgcgtatac
     5161 aagacctaca ggaatgacta accaccattg tgtcttatta ggtagtatac cgagtaagaa
     5221 gtagtcgatg aaaccacctg agaatgtata acctagatga agatctaata agtacaatgt
     5281 taagaatgat aaaccatcaa gtactgcgtg aataaagaat aataatggtg ctacaaataa
     5341 gaatgagaat tctaatggtt ctgtaatacc agttaagaat gatgttaaag cagcagaacc
     5401 cattaaacct gctactactt tcttattttc aggtttagct gtgtgataaa ttgctaaagc
     5461 tgctgcaggt aaaccgaaca tcataacagg gaattcacct tgcatgaatt taccagctgt
     5521 caaatgtgcg ccttcacgaa tttgttcgat aaagatacgt tggtcaccgt gaataatttc
     5581 accagctgca tttttccatg aaccaaactc gaaccagaac ggtgcgtgga aaatgtgatg
     5641 tagaccgaat ggaattaata aacgcttgat gaaaccaaat aagaatacgg caacaccagt
     5701 atttgaatct aataatcctg tactgaatgc atttaatcct gattgaatcg ttggccaaat
     5761 taatgccatt gggaatgcta aaataaatga tgttgtagcc atcataatag gtacgaaacg
     5821 cttaccagcg aagaaaccta aataagatgg taagttaatg ttatagaact tgttataaca
     5881 ccaagctgcc agggccccga ttataatacc gccgaacaca cctgtttgta atgttgggat
     5941 acctaaaatg ctagcgtaac cactcgctgg atcaccaata ttcttaggtg taacttgtaa
     6001 aaagtcgccc attgttttgt tcatgattat gtaaccgacg aatgctgcga tagctgctac
     6061 gccatcaccg ccagctaatc cgattgcgac acctaatgcg aaaatcatag gcaagttatc
     6121 aaaaatgata ccaccagcac ctgtcattaa tttagcgaca gtttgtacgc caccattttg
     6181 tataaacggc aagtagtgtt gtaatgattc accttgcata gctgtaccga tagctaataa
     6241 cagaccagct gctggtaaaa tcgcaacagg taacattagc gctttaccaa tacgttgcaa
     6301 ttgaccgaaa agtttcttcc tcacttgtcc aacctccaaa gttgtattta ttatttaaga
     6361 ttcaataaaa aaagacacga gcaaatagac tgtgttggta accatcacag cttaatttaa
     6421 ctcatgccta atcttactta gtaacacgtt ggtgtatgta attaatgtaa aagaagcaat
     6481 tgactaagta gtatataaaa ctgatacgtt agtttatcta gcttaccatc acatcttatt
     6541 gaatcttatt tgtttgcgac tcctatttta gcatggtgaa gtatgcgttt tcaatacaat
     6601 tattaaattc aatttcaaat ct
//
LOCUS       DAXWFK010000136         6609 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.137, whole genome shotgun sequence.
ACCESSION   DAXWFK010000136 DAXWFK010000000
VERSION     DAXWFK010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6609
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.137"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            105..902
                     /locus_tag="ACGEXS_002084"
     CDS             105..902
                     /locus_tag="ACGEXS_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFF8013077.1"
                     /translation="MRIMFIGDIVGKIGRDAIETYIPQLKQKYKPTVTIVNAENAAHG
                     KGLTEKIYKQLLRNGVDFMTMGNHTYGQREIYDFIDEAKRLVRPANFPDEAPGIGMRF
                     IQINDIKLAVINLQGRAFMPDIDDPFKKADQLVKEAQEQTPFIFVDFHAETTSEKYAM
                     GWHLDGRASAVVGTHTHIQTADERILPKGTGYITDVGMTGFYDGILGINKTEVIERFI
                     TSLPQRHVVPNEGRSVLSGVVIDLDKEGKTKHIERILINDDHPFSTF"
     gene            1042..2802
                     /locus_tag="ACGEXS_002085"
     CDS             1042..2802
                     /locus_tag="ACGEXS_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HFF8013078.1"
                     /translation="MKPQLSWKVGGQQGEGIESTGEIFATAMNRKGYYLYGYRHFSSR
                     IKGGHTNNKIRVSTTPVHAISDDLDILIAFDQETIDVNHHEMREDSIILADAKAKPVK
                     PEGCHAQLIELPFTATAKELGTALMKNMVAIGATSALMNLNTNTFEELITNMFSKKGD
                     KVVEVNIQALNEGYQLMQSRLPEIYGDFELESTDALPHLYMIGNDAIGLGAIAAGSQF
                     MAAYPITPASEVMEYMIANISKVNGAVIQTEDEIAAVTMAIGANYGGVRAFTASAGPG
                     LSLMMEAIGLSGMTETPLVIINTQRGGPSTGLPTKQEQSDLMQMIYGTHGDIPKIVVA
                     PTDAEDAFYLTMEAFNLAEQYQCPVIVLSDLQLSLGKQTVEKLDYNRIEIKRGEIIQS
                     DIEREEDDKGYFKRYALTSDGVSPRPIPGVKGGIHHITGVEHNEEGKPSESASNRQQQ
                     MEKRMRKIEQLLIESPVEANLQHEDADILYIGFISTKGAIQEGSNRLNQQGIKVNTIQ
                     IRQLHPFPTSVIQDAVNKAKKVVVVEHNYQGQLASIIKMNVNIHDKIENYTKYDGTPF
                     LPHEIEEKGKIIATEIKEMV"
     gene            2803..3669
                     /locus_tag="ACGEXS_002086"
     CDS             2803..3669
                     /locus_tag="ACGEXS_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HFF8013079.1"
                     /translation="MATFKDFRNNVKPNWCPGCGDFSVQAAIQKAAANIGLEPEEVAI
                     ITGIGCSGRLSGYINSYGVHSIHGRALPLAQGVKMANKDLTVIASGGDGDGYAIGMGH
                     TIHALRRNMNMTYIVMDNQIYGLTKGQTSPSSAVGFVTKTTPKGNIEKNVAPLELALS
                     SGATFVAQGFSSDIKGLTKLIEDAINHDGFSFVNVFSPCVTYNKINTYDWFKEHLTSV
                     DDIENYDSTDKQLATKTVIEHESLVTGIVYQDKETPSYESQIKELDDTPLAKRDIKIT
                     EDTFNALTEQFI"
     gene            3763..4056
                     /locus_tag="ACGEXS_002087"
     CDS             3763..4056
                     /locus_tag="ACGEXS_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HFF8013080.1"
                     /translation="MKDTLMSIQIIPKTPNNYNVIPYVDEAIKIIDESGLHFRVGPLE
                     TTVQGNMNECLILIQSLNERMVELECPSIISQVKFYHVPDGITIETLTEKYDE"
     gene            4190..5734
                     /gene="miaB"
                     /locus_tag="ACGEXS_002088"
     CDS             4190..5734
                     /gene="miaB"
                     /locus_tag="ACGEXS_002088"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFF8013081.1"
                     /translation="MNEEQRKASSVDVLAERDKKAEKDYSKYFEHVYQPPNLKEAKKR
                     GKQEVRYNRDFQIDEKYRGMGNERTFLIKTYGCQMNAHDTEVIAGILEALGYQATTDI
                     NTADVILINTCAIRENAENKVFSEIGNLKHLKKERPDILIGVCGCMSQEESVVNKILK
                     SYQNVDMIFGTHNIHHLPEILEEAYLSKAMVVEVWSKEGDVIENLPKVREGNIKAWVN
                     IMYGCDKFCTYCIVPFTRGKERSRRPEDIIDEVRELAREGYKEITLLGQNVNSYGKDL
                     QDIEYDLGDLLQAISKIAIPRVRFTTSHPWDFTDHMIDVISEGGNIVPHIHLPVQSGN
                     NAVLKIMGRKYTRESYLDLVKRIKDRIPNVALTTDIIVGYPNESEEQFEETLTLYDEV
                     GFEHAYTYLYSQRDGTPAAKMKDNVPLNVKKERLQRLNKKVGHYSQIAMSKYEGQTVT
                     VLCEGSSKKDDQVLAGYTDKNKLVNFKAPKEMIGKLVEVRIDEAKQYSLNGSFIKEVE
                     PEMVIQ"
     gene            5735..6100
                     /locus_tag="ACGEXS_002089"
     CDS             5735..6100
                     /locus_tag="ACGEXS_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RicAFT regulatory complex protein RicA family
                     protein"
                     /protein_id="HFF8013082.1"
                     /translation="MYNKDDVLNQADNIANKIKNLDTIKTYQQIEAQIHQNQTIKTKM
                     DMLKKHQKQAVNFQNYGKQNALEQSEHTIQSIEAEINTLPIVEQFQTSQYEANQLLKM
                     FVSTMETRLNDHNKAKHSD"
     gene            6127..>6609
                     /gene="thiW"
                     /locus_tag="ACGEXS_002090"
     CDS             6127..>6609
                     /gene="thiW"
                     /locus_tag="ACGEXS_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HFF8013083.1"
                     /translation="MKSRKLAITALLIAINVVLSSIIIIPLGPVKAAPVQHFVNVLSA
                     VIVGPWYGLAQALISSILRVLFGTGTAFAFPGSMIGVLLASMFYIYRKHIFMAAVGEV
                     LGTGVIGSLICIPLAYFLGLQDFFIKPLMITFIVSSAIGSIISYFLLITLKKRGILQR
                     F"
BASE COUNT         2448 a          920 c         1268 g         1973 t
ORIGIN      
        1 atgtctctct ataattgaag aaaaataatt caaagaattt aatatttact ttaaatatta
       61 gaacgtatga attaaactag tatttatgag aggatgaaca aaacatgaga ataatgttta
      121 taggggatat cgtaggtaaa attggacgag acgcaattga aacgtacata cctcaactga
      181 agcaaaagta taaaccaaca gttacaattg taaatgctga aaatgcagca catggtaaag
      241 gtttgactga aaaaatatat aaacaattac taagaaatgg tgtagatttc atgactatgg
      301 gtaatcacac atatggtcaa cgtgaaattt atgattttat agatgaagca aaacgactag
      361 taagaccagc gaattttccg gatgaagcgc cgggaattgg tatgagattt atacaaatta
      421 atgatattaa acttgcagtt attaatctgc aaggaagagc gtttatgcca gatattgatg
      481 atccttttaa aaaggcagat caattagtca aggaagcaca agaacaaact ccgtttatat
      541 ttgttgattt tcatgcagaa acaacttctg aaaagtatgc aatgggatgg catttagatg
      601 gtagagctag cgctgttgtt ggaacgcata cacacattca aacagcagat gaacgtattt
      661 taccaaaggg gacagggtat ataacggatg ttggtatgac aggtttttat gatggcattt
      721 taggaataaa taaaacagag gtaattgagc gttttatcac tagtttgcca caaagacatg
      781 ttgttccaaa tgaaggtaga agtgtattat ctggtgttgt tattgattta gacaaagaag
      841 gtaaaacaaa gcacatcgaa cgtatattga taaatgatga ccatccattt tcaacatttt
      901 aaaattacgt aagtaaacat tcgaattgga ccctatcgtc cattagtatg aatttaatat
      961 agtaccactg tttacatagt aaatcggtgg ttctttttgt tatcatttaa tatgaaatat
     1021 atccatagga ggcatataac tatgaaacca caattatcgt ggaaagttgg cggtcaacaa
     1081 ggcgaaggta ttgaatcaac tggggaaatc ttcgctacgg ctatgaatag aaaaggatat
     1141 tatttatatg gatatagaca tttttcaagt cgtatcaaag gtggacatac gaataataaa
     1201 attagagttt ctacgacgcc tgttcatgca attagtgatg atttagatat tttgattgca
     1261 tttgaccaag aaacaattga tgttaaccat catgaaatga gagaagacag tattatttta
     1321 gctgatgcca aggctaaacc tgtgaaacca gaaggatgtc atgcacagct tattgaatta
     1381 ccttttacag caaccgctaa agaattaggt acagcattaa tgaaaaacat ggttgcaata
     1441 ggtgctacta gcgcattgat gaatttgaat acaaatacat ttgaagaact tattactaat
     1501 atgttttcta aaaaaggtga caaggtagtt gaagtcaata tccaagcatt aaacgaaggt
     1561 tatcaattaa tgcaatctcg cttacctgaa atctacgggg actttgaatt agagtcaaca
     1621 gatgcactac cacatctata tatgattggt aacgatgcca ttggattagg tgcaattgct
     1681 gcaggttcac aatttatggc ggcatatcct attacacctg cgtctgaagt tatggaatat
     1741 atgattgcca atatatctaa agtaaacgga gcggttattc aaacagaaga tgaaattgct
     1801 gctgtaacta tggctattgg tgcaaattat ggtggagtca gagcgtttac ggctagtgct
     1861 ggtccaggtt tatctttaat gatggaagca attggattat ctggtatgac tgaaacgcca
     1921 ttagtcatta ttaataccca acgaggtgga ccttctactg gattacctac gaaacaagaa
     1981 cagtcagatt taatgcaaat gatttatggt acacatggtg atattccaaa aattgttgta
     2041 gcaccaacag atgcagaaga tgcattttat ttaactatgg aagcatttaa tttagcagaa
     2101 caatatcaat gccctgttat agttctaagt gatttgcaat tatctttagg taaacaaact
     2161 gttgaaaaat tagattataa tcgtattgaa attaaacgtg gtgaaatcat tcaatctgat
     2221 attgaacgtg aagaagatga taaaggttat ttcaagcgtt atgcgttaac atccgatggt
     2281 gtttctccta gacctatccc cggtgttaaa ggaggtattc atcatataac tggtgtggaa
     2341 cacaatgaag aaggtaaacc tagtgaatct gcgtcaaata gacaacaaca aatggaaaaa
     2401 cgaatgcgta aaattgagca gttactaatt gaatcgccag tagaagctaa cttacaacat
     2461 gaggatgcag atattcttta tatcggtttt atttctacaa aaggtgcaat tcaagaaggt
     2521 agtaaccgtt tgaatcaaca aggcataaaa gttaacacta tacaaattag acaattgcat
     2581 ccattcccaa caagcgttat tcaagatgca gttaataaag cgaagaaagt cgttgtagtg
     2641 gagcacaatt atcaaggaca attggctagt attataaaaa tgaatgtcaa tattcatgat
     2701 aagattgaaa attatacaaa gtatgatggg acacctttcc taccacatga aatcgaagaa
     2761 aaaggcaaaa taattgctac tgaaataaag gagatggtat agatggcgac atttaaagat
     2821 tttagaaata atgttaagcc taactggtgc cccggatgtg gcgatttctc agtacaagct
     2881 gcaattcaaa aagcagccgc aaatataggg ttagaacctg aagaagtagc tatcatcacc
     2941 ggtataggat gttctggccg tctttcagga tatattaatt cttatggcgt tcattctatt
     3001 cacggacgtg cattaccttt agctcaaggt gtaaaaatgg cgaataaaga tttaactgtt
     3061 attgcatcgg gaggagatgg tgatggttat gctataggta tggggcatac aatccatgct
     3121 ttaagaagaa atatgaacat gacgtatata gtcatggata atcaaattta tggtttgaca
     3181 aagggacaaa catcgccgtc atcagcagta ggatttgtta ctaaaacaac gccaaaaggt
     3241 aatatagaaa aaaatgttgc gcctttagaa ttagcattat catctggtgc cacatttgta
     3301 gcccaaggtt tttcaagcga tattaaagga ttaacaaaac taattgaaga tgcaattaat
     3361 catgatggat tttcattcgt taatgtcttt tcaccatgtg tgacttataa taaaattaac
     3421 acatacgatt ggtttaaaga acatttaaca agtgttgatg acattgaaaa ttatgattct
     3481 acagataaac aattagcgac taaaactgtt attgaacatg aatctttagt aactggtatt
     3541 gtttatcaag ataaagaaac accatcatat gaatctcaaa ttaaagagtt agatgataca
     3601 ccacttgcta aaagagatat caaaattact gaagacacct tcaatgcatt aactgaacaa
     3661 tttatttaat aattaaaact gagtcgtata aatgtattta taacagatcc atttatgcta
     3721 ctcagttttt tactattaca aaaaataaag gagtttttaa aaatgaaaga cacattaatg
     3781 agtatacaaa taattcctaa aacaccaaac aattacaatg ttatacctta cgtagacgag
     3841 gcgattaaaa taattgacga atctggtttg cattttagag taggtccgtt agaaacgaca
     3901 gtacaaggaa atatgaatga atgtttaatt ttaatacaat cattaaatga acgaatggtg
     3961 gaacttgaat gtccaagtat tattagccaa gttaagtttt atcatgtgcc agatggcatc
     4021 actattgaaa ctttaactga aaaatatgat gaataacatt aaaagtgaag taaactggat
     4081 ttgaattggc ttgttagaga tgacgtataa ctttaactgt ttttgcactt tatagttaaa
     4141 tttaatataa ttattaaatg atacgggcaa atagaaagga ttttgtaaag tgaacgaaga
     4201 acaaagaaaa gcaagttctg tagatgtttt agctgagaga gataagaaag cagaaaaaga
     4261 ttatagtaaa tattttgaac atgtttatca gccgcctaat ttaaaagaag cgaaaaaaag
     4321 aggtaaacaa gaagttcgtt ataatagaga tttccaaatt gatgaaaaat atcgcggtat
     4381 ggggaacgag cgtacatttt taattaaaac atatggatgt caaatgaatg cacatgacac
     4441 tgaggtcatt gctggtatac ttgaagcatt aggctatcaa gcaacgactg atattaacac
     4501 tgcagatgtt attttaatta atacatgtgc gattagagaa aatgccgaga acaaagtgtt
     4561 tagtgaaata ggtaatttga agcatttgaa aaaagaacga cctgatattt taatcggtgt
     4621 ttgtggttgt atgtcacaag aagagtcagt agtgaataaa attttaaaat cgtatcaaaa
     4681 tgtagatatg atatttggta cacataatat tcatcattta ccagaaattt tagaagaagc
     4741 atacttatct aaagcaatgg ttgttgaagt atggtctaaa gaaggagacg ttattgaaaa
     4801 tcttccaaaa gtccgtgaag gcaacattaa agcatgggtc aatattatgt atggttgtga
     4861 taagttttgt acatattgta ttgttccatt tacaagaggt aaagaacgaa gccgtagacc
     4921 tgaagacatt atagatgaag tacgtgaact tgctcgtgaa ggttacaaag aaataacgct
     4981 tttaggtcaa aatgtaaatt cttatggtaa agatttacag gatatagaat atgacttagg
     5041 agatctttta caagcaattt ctaaaatagc gattccaaga gttcgtttca caacaagtca
     5101 tccttgggac tttacagatc acatgattga tgttatttca gagggtggta atatcgttcc
     5161 gcatatccac ttgccagttc aatctggaaa taatgcagta ttaaaaataa tgggtagaaa
     5221 atatacacga gaaagttatt tggatttagt aaaacgaatc aaagatagaa ttcctaatgt
     5281 agcattaact acagatatta ttgtagggta tccaaatgaa tcagaggaac aatttgaaga
     5341 aactttaact ctgtatgatg aagttggttt tgaacatgca tatacgtact tgtattcaca
     5401 acgtgatggt acgcctgctg ctaaaatgaa agataatgta cctttaaatg tcaaaaagga
     5461 acgattgcaa cgtttgaata aaaaagttgg tcattattca caaatagcta tgagtaagta
     5521 cgaaggacaa actgtaacag tactttgtga aggaagtagt aaaaaagatg atcaggttct
     5581 tgctggctac actgataaaa ataagctagt taatttcaaa gcgcctaaag aaatgattgg
     5641 taaactagtg gaagtacgaa tagatgaagc taaacagtat tcattaaatg gcagttttat
     5701 aaaggaagta gagccggaaa tggtgattca ataaatgtat aataaagatg acgtgttgaa
     5761 ccaagcggat aatattgcaa ataaaattaa aaatttggat actatcaaaa catatcaaca
     5821 aattgaagca cagattcatc agaaccaaac gataaagact aaaatggata tgttaaaaaa
     5881 gcatcaaaaa caagcagtaa actttcaaaa ttacgggaaa caaaatgcgc tagaacagtc
     5941 ggaacatacc attcagagta tagaagcaga aataaataca ttgcccatag ttgaacagtt
     6001 tcaaacttca caatatgaag cgaatcaatt attgaaaatg tttgtatcaa caatggaaac
     6061 acgtttaaat gaccataata aagccaagca tagtgattaa ttacaaacaa aaaggagaac
     6121 aatcatatga aatcaagaaa actggctata actgcacttt taattgcaat aaatgttgta
     6181 ttaagcagta ttatcatcat tcctctagga ccagttaagg cagcaccagt acagcatttt
     6241 gtaaatgtat taagtgcggt catagtaggt ccttggtatg gattagctca agcgcttata
     6301 tcatcaattt taagagttct ttttggtact ggtacagctt ttgcatttcc gggtagtatg
     6361 attggagttt tattggctag tatgttttac atatatcgta aacatatatt tatggccgcg
     6421 gtcggtgaag tacttggaac tggtgtcatc ggaagtttaa tttgtatacc attagcatat
     6481 ttccttgggc ttcaagactt cttcattaaa ccgttaatga ttacgttcat agtctcaagt
     6541 gctatcggat ctattataag ttatttctta ttaattactc taaaaaaacg tggtattctt
     6601 caaaggttt
//
LOCUS       DAXWFK010000137         6568 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.138, whole genome shotgun sequence.
ACCESSION   DAXWFK010000137 DAXWFK010000000
VERSION     DAXWFK010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6568
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.138"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(122..2098)
                     /gene="sfaD"
                     /locus_tag="ACGEXS_002091"
                     /gene_synonym="sfnaD"
     CDS             complement(122..2098)
                     /gene="sfaD"
                     /locus_tag="ACGEXS_002091"
                     /gene_synonym="sfnaD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine--citrate ligase SfaD"
                     /protein_id="HFF8013084.1"
                     /translation="MNLNLIFKEQTLKFNKEEQETYLFLQQHNSDWANIFKEMILQGR
                     DKVTQRLVTSMHRENLVKARTQSKKILSRDLIMLDISTTHILEIQFPQAKQTLYAPIT
                     GEHAFDRIDVEGPFYIKDDITNTITRVHHPNEILECILIEAPDLKNAASDQFQQDLIN
                     SATNMTFAISYQALSMQHDSAPLFNIIENSEDSYLRSEQAVIEGHPLHPGAKLRKGLN
                     ALQTFLYSSEFNQPIKLKIVLIHSKLSRTMSLSKDYDTTVHQLFPDLIKQLENEFTPK
                     FNFNDYHIMIVHPWQLDDVLHSDYQAEVDKELIIEAKHTLDYYAGLSFRTLVPKYPAM
                     SPHIKLSTNVHITGEIRTLSEQTTHNGPLMTRILNDILEKDVIFKSYASTIIDEVAGI
                     HFYNEQDEADYQTERSEQLGTLFRKNIYQMIPQEVTPLIPSSLVATYPFNNESPIVTL
                     IKRYQSAASLSDFESSAKSWVETYSKALLGLVIPLVTKYGIALEAHLQNAIATFRKDG
                     LLDTMYIRDFEGLRIDKAQLNEMGYSTSHFHEKSRILTDSKTSVFNKAFYSTVQNHLG
                     ELILTISKASNDSNLERHMWYIVRDVLDNIFDQLVLSTHKSNQVNENRINEIKDTMFA
                     PFIDYKCVTTMRLEDEAHHYTYIKVNNPLYRENN"
     gene            2200..3393
                     /gene="sfaA"
                     /locus_tag="ACGEXS_002092"
     CDS             2200..3393
                     /gene="sfaA"
                     /locus_tag="ACGEXS_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A export MFS transporter"
                     /protein_id="HFF8013085.1"
                     /translation="MTKYFFSSSFLLFLGNWIGQIGLNWFVLTTYHNAVYLGIVNFCR
                     LVPILLLSVWAGAIADKYDKGRLLRITISSSFLVTAILCVLTYSFTAIPISVIIIYAT
                     LRGILSAVETPLRQAILPDLSDKISTTQAVSFHSFIINICRSIGPAIAGVILAVYHAP
                     TTFLAQAICYFIAVLLCLPLHFKVTKIPEDASRYMPLKVIIDYFKLHMEGRQIFITSL
                     LIMATGFSYTTLLPVLTNKVFPGKSEIFGIAMTMCAIGGIIATLVLPKVLKYIGMVNM
                     YYLSSFLFGIALLGVVFHNIVIMFICITLIGLFSQWARTTNRVYFQNNVKDYERGKVL
                     SIIMMDRGMIPLGSLLMSICADVFGIVRTFSIMGISTICITMVFYFINRKLKLKLEES
                     NHGIS"
     gene            3380..5137
                     /gene="sfaB"
                     /locus_tag="ACGEXS_002093"
     CDS             3380..5137
                     /gene="sfaB"
                     /locus_tag="ACGEXS_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A synthetase SfaB"
                     /protein_id="HFF8013086.1"
                     /translation="MVYLEWAKADRNIQYRVINAIIKERIYPEQTFISQKGSLIEIQY
                     HMHVLTIEVVRKSALERYEFTGDITYLNKGETSLIITLEGLLDVLNHDFDIPISERLR
                     EELIHSRDSLVETYKQMSHRQTLISQSFKFSRLPQDINFFSWLQHVKDSDKTDDLTYS
                     ESLVPEGHPTHPLTKTKLPLTMEEVRAYAPEFEKEIPLQIMMIEKDHVVCTAMDGNDQ
                     FIIDEIIPEYYNQIRVFLKSLGLKSEDYRAILVHPWQYDHTIGKYFEAWIAKKILIPT
                     PFTILSKATLSFRTMSLIDKPYHVKLPVDAQATSAVRTVSTVTTVDGPKLSYALQNML
                     NQYPGFKVAMEPFGEYANVDKDRARQLACIIRQKPEIDGKGATVVSASLVNKNPIDQK
                     VIVDSYLEWLNQGITKESITTFIERYAQALIPPLIAFIQNYGIALEAHMQNTVVNLGP
                     HFDIQFLVRDLGGSRIDLETLQHRVSDIKITNDSLIADSIDAVIAKFQHAVIQNQMAE
                     LIHHFNQYDCVEETELFNIVQQVVAHAINPTLPHANELKDILFGPTITVKALLNMRME
                     NKVKQYLNIELDNPIKKEV"
     gene            5144..6214
                     /gene="sfaC"
                     /locus_tag="ACGEXS_002094"
     CDS             5144..6214
                     /gene="sfaC"
                     /locus_tag="ACGEXS_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A biosynthesis protein SfaC"
                     /protein_id="HFF8013087.1"
                     /translation="MAHVNINISKIKYNAKVLQTVFQSKNMQFTPVIKCIAGDRTIVE
                     SLKALGINHVAESRLDNIISIADQDLTYTLLRTPAKKEISDMIEKVDMSIQTELSTIH
                     QINEVAEVLGKKHKILLMVDWKDGREGVLTYDVLDYIKEIIHLKNIHFVGLAFNFMCF
                     KSDAPSDDDIFMINRFVSAVEREIGYRLKIISGGNSSMLPQLLYNDLGKINELRIGET
                     LFRGVDTTTNQAIAMLYQDAITLEAEILEIKPRVNTQTHESFLQAIVDIGYLDTKVDN
                     ISPMDQHINILGASSDHLMLDLNGQGHYQVGDHISFSLNYEALSHSMYMKNLHKVYID
                     DSKIDTLLQNFDVKSPAMVNHY"
     gene            complement(6315..6440)
                     /locus_tag="ACGEXS_002095"
     CDS             complement(6315..6440)
                     /locus_tag="ACGEXS_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013088.1"
                     /translation="MYHCYIIFALIQLTPFSMTVIPVIQLILSVAILCDELLHLI"
BASE COUNT         2218 a          930 c         1152 g         2268 t
ORIGIN      
        1 cctgtttttt gtgattttta ataatttgaa tatggaaaat gtattattct ctcatttgta
       61 tagattgtat ttaataagtt aatgtaatcc ttgagctcac gattaataaa attctataac
      121 cttaattatt ttctcgatac aaagggttat taactttaat ataagtataa tgatgtgcct
      181 catcttcaag acgcattgtt gtaacacact tataatctat aaatggcgcg aacatggtat
      241 ctttaatttc atttatgcga ttctcattta cttgattaga tttgtgtgtc gaaagtacaa
      301 gttgatcaaa aatgttatct agtacatcac gaacgatata ccacatatgt ctttctaagt
      361 ttgaatcatt tgatgcttta gagattgtaa gaattaattc gcctaaatgg ttttgaacgg
      421 tagaataaaa ggctttgtta aacacagacg tttttgaatc agtaagaatt cttgattttt
      481 catggaaatg agatgtactg tatcccattt catttagttg tgctttatca attcttaaac
      541 cttcaaagtc tctaatatac atcgtgtcca atagaccatc tttccgaaat gttgcaatag
      601 cattttgcaa gtgagcctct aatgcaatgc catatttagt aactagtggg attacgagac
      661 caagcaatgc tttactataa gtttcaaccc acgatttcgc cgatgattca aaatcagaca
      721 atgatgcagc tgattgataa cgtttaatca atgtcacgat aggtgattca ttgttaaatg
      781 ggtaggttgc aactaagctt gaaggaatca atggtgtgac ttcttgtgga atcatttggt
      841 atatattttt tctaaataat gtacctaatt gttcacttcg ttcagtttga tagtctgctt
      901 cgtcttgctc attataaaaa tgaataccag caacttcatc aataattgtt gatgcatagg
      961 acttaaatat gacatctttc tccaaaatat catttaaaat acgtgtcatt agtggaccat
     1021 tgtgcgtcgt ttgttctgat aatgtacgaa tctcacctgt aatatgaacg tttgtcgaca
     1081 atttgatgtg tggcgacata gctgggtatt taggaactaa tgttctgaaa gataaaccag
     1141 cataataatc caacgtatgt tttgcttcaa tgattaattc tttatctact tctgcttgat
     1201 aatcagaatg taatacgtca tctaattgcc atggatgaac aatcataatg tgataatcat
     1261 taaagttaaa ctttggcgta aattcatttt ctaattgttt aattaagtcc ggaaatagtt
     1321 gatgaacagt tgtatcataa tctttagaca gtgacatagt acggcttaat ttactgtgaa
     1381 tcagtactat tttcaactta ataggttgat tgaattctga agaatatagg aatgtttgta
     1441 atgcatttaa acctttacgt aatttagccc caggatgtag cggatgacct tcaataacgg
     1501 cttgctctga acgcaagtaa ctatcttcgc tattttcgat aatattaaat aaaggtgcag
     1561 aatcatgttg cattgacagt gcttgatagc taattgcaaa tgtcatatta gttgcactgt
     1621 ttattaaatc ttgctgaaat tgatcactag cagcattttt taaatctggt gcttcaatta
     1681 aaatacactc aagaatttca tttggatggt gtactcgtgt aatcgtattt gtaatgtcat
     1741 ctttaatgta gaaagggcct tcaacatcaa ttcgatcaaa ggcgtgttct ccagtgatag
     1801 gagcatataa tgtttgctta gcttgtggga actggatttc tagtatatga gtcgttgaga
     1861 tatctaacat aatcaaatca cgactcaata ttttcttact ttgagtgcgc gctttaacta
     1921 agttttcgcg atgcattgat gtgaccaatc tctgagtgac tttatctctt ccttgtaaaa
     1981 tcatctcttt aaaaatatta gcccaatcgc tattatgttg ttgtaaaaat aaatatgttt
     2041 cttgttcttc tttgttaaat tttaatgtct gttctttaaa aattaagttc aagttcataa
     2101 ttcacctcta tgaaatattt tacaaaagca agatagattt gtataatcca tattaatgat
     2161 aatgattctt attatcaaca gaatgcgggt gtaagtttta tgacaaaata tttttttagc
     2221 agttcttttc tactatttct aggtaattgg attggacaaa tagggctaaa ttggtttgta
     2281 cttaccactt atcataacgc agtttatctg gggattgtca atttttgcag acttgtacca
     2341 atattattac taagtgtgtg ggcaggggca attgccgata aatatgataa agggcgattg
     2401 ctgagaatta caatttcatc atcattttta gtaactgcaa ttttatgtgt gctcacgtat
     2461 agtttcactg caattccaat tagcgtcatt attatatatg cgacattaag agggatttta
     2521 agtgcggttg aaacaccttt aagacaagca atcttaccag atttatcaga taaaatatct
     2581 actacacaag ctgtatcatt tcattcattc atcattaata tttgtcgttc aatagggcct
     2641 gccattgctg gtgtcatatt agcagtctat catgcgccaa caacatttct tgcacaggca
     2701 atttgttatt ttatcgcagt tttattatgc ttaccattac attttaaagt aactaaaata
     2761 cctgaagatg catcaagata catgccgtta aaagttatta tagattactt caaattacat
     2821 atggaaggtc gacaaatatt tataacatca ttattgatta tggcgacagg tttttcatat
     2881 acgacacttt taccagtttt gacaaacaaa gtatttccgg ggaaatctga aatatttggt
     2941 atcgctatga cgatgtgtgc cattggtggt attattgcaa cgctagtttt acctaaagta
     3001 cttaaatata ttggtatggt aaatatgtat tatttaagtt catttttatt tggcattgct
     3061 ttgttaggtg tggtatttca caatattgtc atcatgttca tttgtattac attgattggg
     3121 ttatttagtc aatgggcacg tacgacaaat cgcgtttatt ttcaaaataa tgttaaagat
     3181 tatgaacgtg gtaaagtact gagtattatt atgatggata gaggtatgat tccattggga
     3241 agtctattaa tgagtatatg tgcagatgtg tttggcattg ttagaacttt ttcaataatg
     3301 ggaataagta ctatatgcat tacaatggta ttctatttta taaatagaaa gttgaagtta
     3361 aagttggagg aaagtaatca tggtatatct tgaatgggca aaggcagata gaaatattca
     3421 atatcgtgta attaacgcca ttattaaaga acgtatttac cccgagcaaa catttatttc
     3481 gcaaaaagga tctttaattg aaattcagta tcatatgcat gtgttgacta ttgaagttgt
     3541 tagaaaaagt gcattagaac gctatgagtt tacaggtgat attacttatt taaataaagg
     3601 tgaaacgtca ttaattataa ctttagaagg tttattagat gtgttgaatc atgactttga
     3661 tatccctatt tcagagcgac tacgcgaaga gttaatacac agtcgagata gtttagttga
     3721 aacatataag caaatgtctc acagacaaac gttaataagt caaagtttta aattttcaag
     3781 gttaccacaa gatattaact ttttttcatg gttacaacat gtaaaagata gtgataagac
     3841 agatgattta acttattctg agagtttggt accagagggg catccaacac accctttaac
     3901 caaaacgaaa ttgcccttaa ctatggaaga agtacgagca tatgcacctg agtttgaaaa
     3961 agaaatccct ttgcaaatta tgatgattga aaaagaccat gttgtgtgca cagctatgga
     4021 tggtaatgat caatttatta ttgatgaaat aattcccgaa tactacaatc agattcgtgt
     4081 gtttttaaag agtttaggtt tgaaaagtga agactataga gcgattttag tacatccttg
     4141 gcaatatgat catacgatag ggaaatattt tgaagcatgg attgctaaaa aaatattaat
     4201 tccaacgccg tttacaatac tttcaaaagc aactttatca tttaggacga tgtctttaat
     4261 tgataaacca taccatgtta agttgcccgt cgatgcacaa gcaacaagtg ccgttagaac
     4321 agtctcaact gtgactactg tagatggacc aaagttaagt tatgctttac aaaacatgtt
     4381 gaatcaatat ccaggattta aagttgctat ggaaccgttc ggtgaatatg caaatgttga
     4441 taaagatagg gcacgtcagc ttgcttgtat tataagacaa aagcctgaaa ttgatggaaa
     4501 aggtgcaaca gtagttagtg caagtctagt taataaaaat ccaatagatc aaaaagttat
     4561 cgtggatagt tacttagagt ggttaaatca aggaattact aaagaaagta ttacgacatt
     4621 tattgaacga tacgctcaag cattaatccc gcctttaatt gcttttattc aaaattatgg
     4681 aattgcttta gaagcacaca tgcaaaatac agtagtgaac ttggggccac attttgacat
     4741 tcaattttta gtgagagatt taggtggttc tagaattgat ttagaaacat tacaacatcg
     4801 tgtatcagat attaaaatta caaatgatag tttaatagct gattctatag atgcagtgat
     4861 tgcaaaattc caacatgctg ttattcaaaa tcaaatggca gaattaatcc atcattttaa
     4921 tcagtatgat tgtgttgaag aaaccgaatt atttaacata gtacagcaag tagtagcgca
     4981 tgccattaac ccaacactac cacatgcaaa tgagttaaaa gatattttgt ttggaccaac
     5041 aattactgtc aaagcgttgt taaatatgag aatggaaaat aaagtaaagc aatatttaaa
     5101 tattgagtta gataatccga taaaaaaaga ggtgtagtac tacatggcac acgttaacat
     5161 aaatatatcg aagattaaat ataacgccaa agtacttcaa acagtttttc aaagtaaaaa
     5221 tatgcaattc acaccagtaa ttaagtgcat agctggtgac cgtacaattg tagaaagctt
     5281 aaaagcgtta ggtatcaatc atgttgcaga atccagattg gataacataa ttagtattgc
     5341 agatcaggat ttaacatata cgttattgcg tacgcctgcc aaaaaagaga tttcagatat
     5401 gatagaaaaa gttgatatga gtatccaaac agaattatct accatacatc aaataaatga
     5461 agtagcagaa gttttaggaa aaaaacataa gattttatta atggtagatt ggaaagatgg
     5521 ccgtgaaggt gttctgactt atgatgtttt agattatatt aaagagatta ttcacttgaa
     5581 aaatatccat tttgtaggtt tagcattcaa ttttatgtgt tttaaatctg atgcgccaag
     5641 tgatgatgat atctttatga ttaatcgatt cgtctcagct gttgaaagag aaataggata
     5701 tcgattgaaa attatttcag gtggtaattc gagcatgtta ccacaacttt tatataatga
     5761 tttgggtaaa ataaatgaat tgcgaatagg tgaaacgttg tttagaggtg ttgatacaac
     5821 gacaaatcaa gcgatcgcta tgttatatca agatgcgatt acattggagg ctgagatttt
     5881 agaaattaaa cctcgagtta atacacaaac gcatgaatca ttcttacaag ctattgtaga
     5941 tattggttat ttagatacaa aagtcgataa tatttcacct atggatcagc atatcaatat
     6001 tttaggcgct tcgagtgatc atttaatgct tgatttaaat ggtcaaggac actatcaagt
     6061 tggagatcat atatcttttt cattaaatta tgaagcactt tctcattcga tgtatatgaa
     6121 aaatttacat aaggtatata ttgatgattc aaagattgat acgctacttc aaaattttga
     6181 tgtgaaatct cctgctatgg taaaccacta ttaatatatt tatcaataag tctaagttga
     6241 caagttatac tacatatcgt ttgcacggtt gtatcataat tgttcaactt agattttttg
     6301 tatttgttga tttatcaaat taagtgcaac agttcgtcac ataaaattgc aacagataat
     6361 atcagctgaa ttacagggat aacggtcatg ctaaatggtg tcaattgtat taatgcaaag
     6421 atgatatagc aatgatacat tatcagtatt ttgtctaagg aaatgtgcta attgtagtca
     6481 taattattag aggaaatata tagtcataca ttttagaaat ataaaaaaga ttgaacgcta
     6541 cttgacaatg ataattgtta tcaataaa
//
LOCUS       DAXWFK010000138         6487 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.139, whole genome shotgun sequence.
ACCESSION   DAXWFK010000138 DAXWFK010000000
VERSION     DAXWFK010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6487
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.139"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            24..524
                     /gene="ftnA"
                     /locus_tag="ACGEXS_002096"
     CDS             24..524
                     /gene="ftnA"
                     /locus_tag="ACGEXS_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-type ferritin FtnA"
                     /protein_id="HFF8013089.1"
                     /translation="MLSKNLLEALNDQMNHEYFAAHAYMAMAAYCDKESYEGFANFFI
                     QQAKEERFHGQKIYNYINDRGAHAEFRAVSAPKIDFSSILETFKDSLSQEQEVTRRFY
                     NLSEIARQDKDYATISFLNWFLDEQVEEESMFETHINYLTRIGDDSNALYLYEKELGA
                     RTFDEE"
     gene            complement(606..698)
                     /locus_tag="ACGEXS_002097"
     CDS             complement(606..698)
                     /locus_tag="ACGEXS_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013090.1"
                     /translation="MTKNTIISLENEKTQINDSENESSDLRKAK"
     gene            913..1467
                     /locus_tag="ACGEXS_002098"
     CDS             913..1467
                     /locus_tag="ACGEXS_002098"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFF8013091.1"
                     /translation="MIQDAFVALDFETANGKRTSICSVGMVKVIDSQITETFHTLVNP
                     QDYFSQQNIKIHGIQPEDVENAPTFDYVFPYMMQFIADLPVVAHNAAFDMNVLHQSIQ
                     NIGLPTPNLTYFCSYQLAKRTVDSYRYGLKHMMEFYQLDFHGHHDALNDAKACAMITF
                     RLLKNYENLTYVTNIYGKNLKDKG"
     gene            complement(1535..2605)
                     /gene="dinB"
                     /locus_tag="ACGEXS_002099"
     CDS             complement(1535..2605)
                     /gene="dinB"
                     /locus_tag="ACGEXS_002099"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFF8013092.1"
                     /translation="MTERRIIHIDMDYFFAQVEMRDNPKLKGKPVIVGGKASSRGVVS
                     TASYEARKYGVHSAMPMSQAHKLCPNGYFVTSNFGAYRETSAQIMSIFRSYTDKVEPM
                     SLDEAYLDITELVRPDLPASKIAQYIRKDILEQTHLTASAGVSYNKFLAKLASGMNKP
                     DGMTVIDYQNVHDILMTLDIGDFPGVGKASKKVMHDNGIFNGRDLYEKTEFELIRLFG
                     KRGRGLYNKARGIDHSEVKSSRVRKSVGTERTFATDVNDDEEILRKVWELSGKTAERL
                     NKLQKSAKTVTVKIKTYQFETLSKQMSLRDSVSSEEDIYNIAYLLYNDLKDPDVPIRL
                     IGVTVGNLEQSTYKNMTIYDFI"
     gene            complement(2852..3382)
                     /locus_tag="ACGEXS_002100"
     CDS             complement(2852..3382)
                     /locus_tag="ACGEXS_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HFF8013093.1"
                     /translation="MHKIDLTTNNFQMRRFIILQLVIALFVILFTYKWALGVTAVVDQ
                     NIIINLVYGFAGFIILLILHELIHRALFLLFKKDSKPMFNIKKDRILFQTADACFNKW
                     QFSIIMLSPLILLSTGLLILIKVFGYSSLIFMFSMHTAYCFIDILLVALTISSSFKYV
                     QQDEDSIYLYHQKPTQ"
     gene            complement(3552..4913)
                     /gene="rlmD"
                     /locus_tag="ACGEXS_002101"
     CDS             complement(3552..4913)
                     /gene="rlmD"
                     /locus_tag="ACGEXS_002101"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFF8013094.1"
                     /translation="MQAIAKNDIKTGTVVDLTHEGHGVVKIDRFPIFIPQALINEQIE
                     YKIIKVKKNFAIGKLLNINTRSENRVAPPCIYYERCGGCQLQHLSYEAQLEMKKEQVI
                     NLFQRKAHFDNSKINDTVGMTDPWRYRNKSQIPVGKNEQNEVIMGFYRQRSHDIIDME
                     SCLIQDSQHQEVMNEVKSILKDLNVSIYQEQLKKGLMRHLVVRTGYHTDEMMIIFVTN
                     GKKWPQKNAVVEKILDAFPNVTSIKQNINDSHSNVIMGRQSITLYGKDTIIDQLTDST
                     FKISDQSFYQINSEQTEKLYNKAIEYAQLTGNEVVLDTYCGIGTIGLYMAPLAKHVYG
                     VEVVPSAIEDAQQNATINQCNNTTFVCGKAEEVILQWKAQGIKPDVVMVDPPRKGCDE
                     TFIQTLLTLEPKRIVYISCNPATQQRDALLLAEKYQLEEVTPVDMFPQTTHVETVALF
                     NLK"
     gene            complement(4994..5941)
                     /locus_tag="ACGEXS_002102"
     CDS             complement(4994..5941)
                     /locus_tag="ACGEXS_002102"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFF8013095.1"
                     /translation="MRKRARIIYNPTSGKELFKRELPDALIKLEKAGYETSAYATEKI
                     GDATLEAERAMHENYDVLIAAGGDGTLNEVVNGIAEKPNRPKLGVIPMGTVNDFGRAL
                     HIPNDIMGALDVIIEGHSTKVDIGKMNNRYFINLAAGGQLTQVSYETPSKLKSIVGPF
                     AYYIKGFEMLPQMKAVDLRIEYDGNVFQGEALLFFLGLTNSMAGFEKLVPDAKLDDGY
                     FTLIIVEKSNLAELGHIMTLASRGEHTKHPKVIYEKAKAINISSFTDLQLNVDGEYGG
                     KLPANFLNLERHIDVFAPNDIVNEELINNDHVDDNLIEE"
BASE COUNT         2103 a         1172 c          913 g         2299 t
ORIGIN      
        1 ttacaaataa gaggtgtatc aaaatgttaa gtaaaaattt attagaagca ttaaatgatc
       61 aaatgaacca tgagtacttt gcagcacacg catatatggc aatggcagca tactgtgata
      121 aagaatcgta cgaaggattt gcaaacttct tcattcaaca agctaaagaa gaacgtttcc
      181 atggacaaaa gatttataac tatattaacg acagaggtgc acatgcagaa ttcagagcag
      241 tttcagcacc aaaaattgac ttttcaagca tactagaaac tttcaaagac agcttatctc
      301 aagaacaaga agtaacaaga cgtttctata acttatctga aatcgctcgt caagataaag
      361 attatgcaac tatctcattc ttaaactggt tcttagatga acaagtcgaa gaagaatcaa
      421 tgtttgaaac tcacatcaat tatttaactc gtatcggcga tgacagcaat gcattatatc
      481 tttacgaaaa agaacttggc gctcgtacat tcgacgaaga ataattaaac atcactacaa
      541 tagacagata aatatcatac gacatgatag gcatttgggt cacttacaat aacccaatgt
      601 ctatattatt ttgctttacg gagatcacta gattcatttt ctgaatcatt gatctgcgtt
      661 ttttcatttt caaggctaat tattgtattt ttagtcattt attttttaaa ctactaatgt
      721 taataactct aaatttgatg ttgaattaat ttgacgattt taaagcatat catcatttac
      781 tttttaatca gagttacatc caaatgatag atttcacgtt ataccttcac gtataatatt
      841 atgtatcgtt tgtaagcaaa tgactaaaag tctattaata tatacattta attaattgaa
      901 aggattgact acatgataca agatgcgttt gttgcacttg attttgaaac agcaaatggt
      961 aaacgtacaa gtatttgttc tgtcggaatg gttaaagtca ttgatagtca aataacagaa
     1021 acatttcata ctcttgtgaa tccgcaagac tatttttcac aacaaaatat taaaattcat
     1081 ggcatacaac cagaagatgt tgaaaatgca cctacgtttg actacgtatt tccatatatg
     1141 atgcaattta ttgcagattt acctgttgtc gcacataacg cggcatttga tatgaacgtc
     1201 ttacatcaaa gcattcaaaa tattggttta ccaactccaa atttaactta cttttgtagt
     1261 tatcaacttg ctaaaagaac cgttgattcg tatcgatacg gtttaaaaca tatgatggag
     1321 ttttatcaat tagattttca tggtcatcat gatgcattga atgatgccaa agcatgcgca
     1381 atgattactt ttaggctact gaaaaattat gaaaatttaa catatgtaac taatatttat
     1441 ggtaaaaatc taaaagataa aggctaggac taaataaaat actcccttca aaagtaagca
     1501 ttgtaaaaat gtaaactttg cagggagctt tattttatat aaagtcatat atcgtcatat
     1561 ttttataagt tgattgttct aaattaccta cagtgacacc aataagtcga attggtacat
     1621 cagggtcttt taaatcgtta taaagtaaat atgcaatatt ataaatatct tcttcagaac
     1681 taaccgaatc tcttaaactc atctgtttag atagcgtttc aaattgataa gttttaattt
     1741 taaccgttac agttttagct gacttctgta atttatttag acgttcagct gttttacctg
     1801 acaattccca tacttttctt aaaatctctt catcatcatt cacgtctgtt gcaaatgtgc
     1861 gttcagtccc tactgatttt cttactcttg atgatttcac ttcactatgg tcaataccgc
     1921 gtgccttgtt atataaaccc cgacctcttt ttccaaacaa acgtattaat tcaaattccg
     1981 ttttctcata taaatctcta ccgttaaaaa taccattatc atgcattact tttttggaag
     2041 ctttacctac gcctggaaaa tctccaatat ccaatgtcat caaaatatca tggacatttt
     2101 gataatcaat cacagtcata ccatcaggtt tattcatacc actcgctaat ttagctaaaa
     2161 atttgttata agaaacacct gcagatgctg ttaaatgtgt ctgctctaga atatcttttc
     2221 taatatactg agcaattttc gaagcaggaa ggtctggtct cactaattct gtaatatcta
     2281 aatacgcttc atccaatgac atcggttcta ccttatctgt ataacttcgg aaaatagaca
     2341 taatctgcgc agatgtttct cggtaagcac caaaattact tgtgacaaag tatccatttg
     2401 gacataattt atgcgcttgt gacataggca ttgctgaatg gacgccgtat tttcgtgctt
     2461 cataggatgc cgtagagaca acacccctac tgcttgcttt accaccaaca atgactggtt
     2521 tccctttcaa tttggggtta tctctcattt cgacttgtgc aaaaaaatag tccatatcta
     2581 tatgaataat tcgtctctca gtcaagtgct cacctcccta ctaattttta cttttataac
     2641 gcacaaaaat atctcaacat aattatacgc tgtgtacgat ttttttacat aaatcttgca
     2701 cttagcgata actatattga gataactaca agttgttata aaatcaattg ctatttaagc
     2761 atgatgatga agacgattga gtaagaaaac ataggtaatc tgaaataatt caagcaaatt
     2821 cattttgttg gtatcatcat attaaaattt attattgagt cggcttttga tgatacaaat
     2881 aaatactatc ttcatcttgt tgaacatatt taaaactact agatattgtt aatgcaacaa
     2941 gcagaatatc tataaagcaa taagcggtat gcatactaaa cataaaaata agtgatgaat
     3001 aaccaaatac cttaattaaa ataagcaagc cagtacttaa taggattagt ggtgacagca
     3061 taataattga gaattgccat ttgttgaagc aagcatctgc tgtttggaat aagattctgt
     3121 ctttttttat attaaacata ggtttgctat cttttttaaa taaaagaaat aatgctctat
     3181 ggataagttc atgtaaaatc aataaaataa tgaatccagc aaacccatat acaagattga
     3241 tgatgatatt ttgatcgaca accgctgtga cacctaacgc ccacttatac gtaaataaaa
     3301 tcacgaataa cgcaataaca agttgcaaga taataaacct tcgcatttga aaattatttg
     3361 tcgttaaatc aattttatgc attaccaacc ctcccgatca tgacattctt attcttcttt
     3421 aaatatagta tacaatgtca catttaattt aaaaagttca tatcaagaaa gtaaattggc
     3481 tgtaataaaa ttttaatata cgacttcttt cttcacttat taaggcgaaa ttttatctca
     3541 aatcatgtgc gctatttcaa attgaataat gccactgtct caacatgtgt tgtttgtgga
     3601 aacatatcta ccggtgttac ctcttcaagt tgatattttt cagctaataa taatgcatca
     3661 cgttgctgtg ttgcgggatt acatgaaata tagacaatac gcttaggttc taatgtaagc
     3721 aaagtctgaa taaacgtttc gtcacagccc tttcttggcg gatcaaccat tacaacatct
     3781 ggtttaatcc cttgtgcttt ccattgtaaa ataacttctt cagctttccc acagacaaaa
     3841 gttgtattat tgcattggtt tatagtcgca ttttgttgtg cgtcttcaat tgcagaaggt
     3901 actacttcaa caccgtatac atgttttgca agtggtgcca tatatagccc tattgttcca
     3961 ataccacaat aggtatctaa tacaacttca ttacctgtca attgcgcata ctcaattgct
     4021 ttattatata atttctctgt ttgttcagaa ttaatttggt agaatgactg atcacttatt
     4081 ttaaatgtac tatctgttaa ttgatcaata attgtatctt taccatatag cgttatagat
     4141 tgacgtccca taataacatt agagtggcta tcattaatgt tttgtttaat gcttgtcaca
     4201 ttaggaaatg catctaatat cttctcaaca acagcatttt tttgtggcca ctttttacca
     4261 ttagttacaa aaataatcat catttcgtct gtatgatatc ctgttcttac aaccaaatgt
     4321 ctcattaaac cttttttcaa ttgttcttga taaatactta catttaaatc ttttaaaata
     4381 gatttaactt cattcatcac ttcttgatgt tgtgaatctt gtattaaaca actttccatg
     4441 tcaataatgt catggcttct ttgacgataa aagcccataa taacttcatt ctgttcattc
     4501 ttaccaactg gaatctggga cttgtttcga tatctccaag gatctgtcat gccaactgta
     4561 tcgttaatct tagaattatc aaaatgcgct tttcgctgaa acaaattaat cacttgttcc
     4621 tttttcattt caagttgtgc ttcgtatgat aagtgttgaa gttggcaccc accacaacgt
     4681 tcataatata tacatggcgg tgcaacacga ttctcacttc tcgtatttat attaagcaac
     4741 ttacctatcg caaagttctt ttttactttg ataattttat attcaatttg ttcattaatt
     4801 aaagcttgtg gtatgaaaat aggaaagcga tctattttta cgacaccatg gccttcatgc
     4861 gttaaatcaa caactgttcc cgtttttatg tcatttttag ctattgcttg caaaatttta
     4921 cctccaaaat gaacaggtta ggaacaaaat tatgcgcttc ctaacctgcc attatatatt
     4981 tcactatttc tgtttattct tcgattaaat tgtcatcaac atgatcatta tttattaact
     5041 cttcatttac aatatcatta ggtgcaaaga catctatatg acgttctaga tttaagaaat
     5101 tcgctggtaa tttaccacca tattctccat ctacatttag ttgtaagtct gtgaatgatg
     5161 aaatattaat tgcctttgct ttttcataaa taactttagg atgcttagta tgttctcctc
     5221 ttgaagctaa agtcataata tgaccaagtt ctgcaaggtt tgatttttca actataatta
     5281 acgtaaaata gccgtcatct aacttagcgt ccggcactaa tttttcaaat cctgccattg
     5341 aatttgttaa acctaaaaag aataataatg cttctccttg gaaaacatta ccatcatatt
     5401 caattcttaa atctacagct ttcatttgag gtaacatttc gaaacctttg atgtaataag
     5461 caaatggacc aacaatagat ttcaatttac tcggtgtttc ataagagact tgcgtcaatt
     5521 gtccgcctgc agctaaatta ataaagtatc gattattcat tttaccaata tctactttag
     5581 tagaatgacc ttcaatgatg acatcaagtg cccccatgat gtcattaggt atatgcaatg
     5641 cacgtccaaa gtcattaaca gtacccatag gaatgacacc tagcttagga cgattaggct
     5701 tttctgcgat accattaact acttcattta atgttccatc accacctgca gcgattaata
     5761 catcataatt ttcatgcata gctctttctg cttcaagtgt ggcatcacct attttctcgg
     5821 ttgcatatgc actcgtttca tatcccgctt tttctaattt tattaaggca tcaggtaatt
     5881 ctcttttaaa tagctcttta cctgatgtcg ggttataaat gattctagca cgtttcctca
     5941 tatcttatcc ctctacttaa aattcatata ttttaacttc atctttgttt cgtctaatag
     6001 ggagtgggac agaaataata tttaacaaaa tttatttcgt tctaccccaa cttgcattgt
     6061 ctgtagaatt tcctttcgaa attctctatg ttggggcccc accccaactt gcacattatt
     6121 gtaagctgac agaaagtcag cttctatgtt ggggccccac tagaattgaa aaaagcttgt
     6181 tacaagcgta ttttctttca gtcaactaca gccaatataa cattgtagtg cctaggacat
     6241 tgaatttatg acccaggctc agtcttattt catcattctt aatatcgtta aagaccaact
     6301 tgtatcttaa acaaatacta tctcaatatg tacaaagctt gttatttatt cagcattttt
     6361 tgccgttctt cattatatag acttcgtcag ttatgctatt ttacctttaa aatgatgttg
     6421 taaatataat gttgtcttta tacaaaaatg ttttatacct ttttattata cagtttgcct
     6481 taatata
//
LOCUS       DAXWFK010000139         6461 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.140, whole genome shotgun sequence.
ACCESSION   DAXWFK010000139 DAXWFK010000000
VERSION     DAXWFK010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6461
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.140"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(144..644)
                     /locus_tag="ACGEXS_002103"
     CDS             complement(144..644)
                     /locus_tag="ACGEXS_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFF8013096.1"
                     /translation="MITYKNILIAVDGSHEAEWAFNRAVGVAKRNDAKLTIVNVIDSR
                     TYSSYEVYDAQFTEKSKHFAEELLNGYKEVATNAGVKDVETRLEFGSPKSIIPKKLAH
                     EINADLIMSGTSGLNAVERFIVGSVSESIVRHAPCDVLVVRTEELPADFQPQVATTQL
                     REKYQN"
     gene            785..1903
                     /gene="ald"
                     /locus_tag="ACGEXS_002104"
     CDS             785..1903
                     /gene="ald"
                     /locus_tag="ACGEXS_002104"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0042853 - L-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HFF8013097.1"
                     /translation="MKIGIPREIKNNENRVGLSPSGVHALVESGHTVLVETNAGSGSF
                     FEDVDYKEAGAEIVAEQAKVWDVDMVIKVKEPLESEYPYFKEGLVLFTYLHLANEEKL
                     TQALIDRKVISIAYETVQLPDRSLPLLSPMSEVAGRMSAQVGAEFLQKLNGGMGILLG
                     GVPGVPKGKVTIIGGGQAGTNAAKIALGLGADVTILDVNPKRLQQLDDLFGGRVHTIM
                     SNPLNIELYVKQSDLVIGAVLIPGAKAPRLVTEDMIKQMKNGSVIIDIAIDQGGIFET
                     TDKITTHDDPTYIKHGVVHYAVANMPGAVPRTSTLALNNATLPYALMLANKGYREAFK
                     SNQPLSLGLNTYKGHVTNKGVAEAFEMEYKSVEEALQL"
     gene            2027..2125
                     /locus_tag="ACGEXS_002105"
     CDS             2027..2125
                     /locus_tag="ACGEXS_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013098.1"
                     /translation="MNDIDLIHYMLLMNIPAYVFLACLLHIAFRLG"
     gene            complement(2219..3274)
                     /locus_tag="ACGEXS_002106"
     CDS             complement(2219..3274)
                     /locus_tag="ACGEXS_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFF8013099.1"
                     /translation="MTKISKIIDELNNQQADAAWITTPLNVYYFTGYRSEPHERLFAL
                     LIKKDGKQVLFCPKMEVEEVKASPFTGEIVGYLDTENPFSLYPQTINKLLIESEHLTV
                     ARQKQLISGFNVNSFGDVDLTIKQLRNIKSEDEISKIRKAAELADKCIEIGVSYLKEG
                     VTECEVVNHIEQTIKQYGVNEMSFDTMVLFGDHAASPHGTPGDRRLKSNEYVLFDLGV
                     IYEHYCSDMTRTIKFGEPSKEAQEIYNIVLEAETSAIQAIKPGIPLKDIDHIARNIIS
                     EKGYGEYFPHRLGHGLGLQEHEYQDVSSTNSNFLEAGMVITIEPGIYVPGVAGVRIED
                     DILVTNEGYEVLTHYEK"
     gene            3449..4138
                     /locus_tag="ACGEXS_002107"
     CDS             3449..4138
                     /locus_tag="ACGEXS_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFF8013100.1"
                     /translation="MKLSFHGQSTIYLEGNNKKVIVDPFISNNPKCDLNIETVQVDYI
                     VLTHGHFDHFGDVVELAKKTGATVIGSAEMADYLSSYHGVENVHGMNIGGKANFDFGS
                     VKFVQAFHSSSFTHENGIPVYLGMPMGIVFEVEGKTIYHTGDTGLFSDMSLIAKRHPV
                     DVCFVPIGDNFTMGIDDASYAINEFIKPKISVPIHYDTFPLIEQDPQQFKDAVNVGDV
                     QILKPGESVQF"
     gene            complement(4443..4856)
                     /locus_tag="ACGEXS_002108"
     CDS             complement(4443..4856)
                     /locus_tag="ACGEXS_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFF8013101.1"
                     /translation="MYKNILLGVDTQLKNEKALKEVSKLAGEGTVVTVLNAISEQDAQ
                     ASIKAGVHLNKLTEERSKRLEKTRKALEDYGIDYDQIIVRGNAKEELLKHANSGKYEI
                     VVLSNRKAEDKKKFVLGSVSHKVAKRATIPVLIVK"
     gene            5061..6359
                     /locus_tag="ACGEXS_002109"
     CDS             5061..6359
                     /locus_tag="ACGEXS_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFF8013102.1"
                     /translation="MTKHEQILDYIESLSIGSKISVRKIAKFLNVSEGTAYRAIKDAD
                     KMGMVATIDRVGTVRIEKRNRNEIEHLTFNEIVNIIDGQVLGGNKGITKMVSKFAIGA
                     MELKDILKYIGPKTLLIVGNREDVQIEALKRGTAILITGGFKPSNKVINFANEHDLPV
                     LSSSYDTFLVANIINKALFNQKIRKDILIVQDIMTPLDDLSVLFDTMKIADYKRMANR
                     TGHTRFPVVNESYKLVGIVTSREMINTKDDDEIDKVMTRNPIYVNAMSTVASCAHMMI
                     WEGIELIPVVSSNKKTVGVINRQDVLKSMQLLGRQPQMGETINDQIAKYITMNQDGIT
                     VEVSPLLINHYGTVSKAAFVSIIEETIQYEMRKFKKGNVMIENLNIVYIKTVPIESHI
                     TVRFGILDVGRNFAKIEVNMHSQNDKVASALVICQMFDEV"
BASE COUNT         2130 a          984 c         1165 g         2182 t
ORIGIN      
        1 cgaatatctt aaagaattaa gtagtcgaag aagattatga aaacattatt aagtgagttt
       61 tatttataaa caataaaaaa ccaagtgcag aaagttgcac ttggttcata atgatcaagt
      121 agttatctaa tgatcatcta tatttaattt tgatattttt cacgtaattg agttgttgca
      181 acttgtggtt ggaagtctgc tggtaactct tcagtacgaa caactaacac gtcacatggc
      241 gcatgacgaa cgatagattc tgatacagaa ccaacaataa atctttccac ggcatttaag
      301 cctgatgtac cactcataat caagtctgca ttaatttcat gtgcaagctt tttaggaatg
      361 atagatttag gagagccaaa ctctagacgc gtttctacat ctttaacacc agcgttagta
      421 gctacttctt tataaccatt taataattct tctgcaaaat gcttagattt ttcagtaaat
      481 tgagcatcat aaacttcata agaagaatac gttcttgaat caattacatt cacaattgtt
      541 aacttcgcat cgttacgttt agcaacacca actgctctgt taaatgccca ttccgcttca
      601 tgtgaaccgt caactgcgat taaaatattt ttgtaagtaa tcatagtaag tgcctccttg
      661 ttctttctta attatatttt accacaattt tcggaaaagt cagtgtaata acctttacaa
      721 ttttgtggat ttttgattgc gctttcaaaa tgttcgcgtt aagatgatta tggaggtgga
      781 tggtatgaaa attggtatac caagggagat taaaaataat gaaaatcgtg ttggtttatc
      841 accaagtggt gtgcacgctt tagtagaaag tgggcatact gttttagtgg aaacaaatgc
      901 gggttcagga tcattctttg aagatgtaga ttacaaagaa gcaggtgctg agattgttgc
      961 tgaacaagca aaagtttggg atgtggatat ggttattaaa gttaaagaac cacttgaatc
     1021 tgaatatcca tattttaaag aagggcttgt attattcact tatcttcatt tagcaaatga
     1081 agaaaaatta acacaagctt tgatagatag aaaagtaatt agtattgcat atgagactgt
     1141 gcagttacca gaccgatctt taccattgtt atcaccaatg agtgaggtag caggaagaat
     1201 gtcagctcaa gttggcgcag agttcctaca aaaacttaat ggtggtatgg gaattctact
     1261 aggtggtgtc ccaggagtac ctaagggtaa agtaactatt atcggtggtg gtcaagcagg
     1321 aacaaatgca gctaaaattg cactaggact aggtgcagat gttacgattt tagatgttaa
     1381 tccaaagcgt ttacaacaat tagatgattt attcggtgga cgtgtacata caattatgtc
     1441 aaatccgttg aatattgagt tgtatgttaa acaaagtgat ttagtaatag gtgcagtttt
     1501 aattccaggt gctaaagcgc caagacttgt aacagaagac atgattaaac aaatgaaaaa
     1561 tgggtcagtt attattgaca ttgctattga tcaaggcggt atttttgaaa caactgataa
     1621 aattacgaca catgatgatc ctacatatat taagcatggt gtggttcatt atgcagttgc
     1681 aaatatgcca ggtgcagtac cgcgtacttc gacgttagct ttaaataatg ctacgctacc
     1741 ttatgcgctc atgctagcta ataaagggta tagagaagca tttaaatcaa atcaaccatt
     1801 atcattaggt ttaaatactt acaaaggtca cgtaaccaat aaaggcgttg cagaggcatt
     1861 tgaaatggaa tataaatctg tagaagaagc attacaatta taatttgaat ttgatgaaaa
     1921 gggaatggat tacagcgtac taaagaatga cgttatcatc tccttggtta ttaaaattaa
     1981 ataatgataa attaaagcta gaaacaatgc gaagaaatca tcgaacatga atgatattga
     2041 tttgattcat tacatgttac tgatgaacat acctgcatat gtgtttctag cttgtttgtt
     2101 acatattgca tttagacttg gataaaaatt tatgtcttag gcgctacaat gttatattgg
     2161 cagtgtttga ctgaatgaga atgcgcttgt aacaagcttt tcatttttat cccactcctt
     2221 atttttcgta atgtgttaat acttcatatc cttcattagt gacaagtatg tcatcttcaa
     2281 ttcttacacc tgcaacacca ggtacataaa tacctggttc gattgtaata accatgccag
     2341 cttctaaaaa attagaatta gtacttgaaa catcttgata ttcatgttct tgtaatccta
     2401 ggccatgacc taagcgatga gggaaatatt caccataacc tttttctgaa ataatatttc
     2461 tagcgatatg atcgatatct tttaatggta ttccaggttt aattgcttgg attgcagatg
     2521 tttctgcttc taatacaata ttataaattt cttgtgcttc tttgctaggt tcaccaaatt
     2581 taatagtacg tgtcatatcg ctacaataat gctcataaat tacacctaaa tcaaatagta
     2641 catattcatt gctttttaat ctgcgatctc ctggtgtgcc atgaggtgat gcggcatgat
     2701 ctccaaataa aaccatcgta tcaaaactca tttcattgac gccatattgt ttgatagttt
     2761 gctcaatatg gttgactact tcacattcag tcacaccttc ttttaaataa gaaacaccta
     2821 tttcgataca cttatctgct aactcagcag ctttacgtat tttgctaatt tcatcttcgg
     2881 atttaatatt tctcaattgt ttgattgtta aatcaacatc tccgaatgaa ttgacattga
     2941 aaccagagat taattgtttt tggcgtgcta ctgttaagtg ctcgctttca attagtaatt
     3001 tattgattgt ttgaggataa agtgaaaaag ggttttcagt gtctaaatat ccaacgattt
     3061 cacctgtgaa aggtgatgct ttgacttctt cgacttccat ttttggacaa aatagtactt
     3121 gtttaccatc tttcttaatc aataatgcaa ataatctttc atggggttcg ctacggtatc
     3181 cagtaaaata atatacattc aacggtgttg taatccatgc tgcatcagct tgttgattgt
     3241 tcaattcgtc tattattttt gatatttttg tcattttata aacactcctc aacataatat
     3301 tacttccatt ttatgccaat tattattaga tttcaaaatc gagctaatac attaactaga
     3361 ttttcaattt ttaaaatttg agaatttgtt ataattaagt ggcaaaaggg tactaaattt
     3421 atatagtaat tgataggagg atgctcaaat gaagttatca tttcatggtc aatcaacgat
     3481 ttatttagaa gggaacaaca aaaaggttat cgtagatccg tttatttcaa ataaccctaa
     3541 gtgtgatttg aatattgaaa cagtgcaggt agactatatc gttttaacgc acggtcattt
     3601 tgatcatttt ggtgatgttg tggaactagc aaagaaaaca ggagcaacag taattggaag
     3661 tgcagaaatg gctgactatc tttcttcata tcacggtgtt gaaaatgttc atggtatgaa
     3721 tattggtggt aaagcaaact ttgatttcgg aagcgttaaa tttgttcaag catttcatag
     3781 ttcaagtttt acacatgaaa atggtattcc agtttattta ggtatgccaa tgggtatcgt
     3841 gtttgaagtt gaaggaaaaa caatttatca tactggtgat acaggtctgt ttagtgacat
     3901 gtcacttatt gcgaaacgtc atccagttga tgtatgtttt gtaccaattg gagataattt
     3961 tacgatgggt attgatgatg caagttatgc tattaatgaa tttattaaac caaaaatttc
     4021 tgtaccgatt cattatgata cattcccatt aatcgaacaa gatccacaac aatttaaaga
     4081 tgctgttaat gtaggggacg tgcaaatttt aaaaccaggt gaatctgttc agttttaaat
     4141 agaatataga ttttacacta aaaaacacct cgctaatcta tgtggaagtt atcgtctgca
     4201 tatttttagt gggtgttttt atattctact gattcagtta cgacgtggac gggggacatc
     4261 agtggttctt tatcatttct gacctactct cgtaatttta gttttataca attagacatt
     4321 gattataaat ttactacgat ataaaaaatc cagaaccaca gtcataatcg tttcttgtat
     4381 ttactcagtc gtttgagact gcatcatgat tgaaagatta tttgtgattc tggataaaaa
     4441 ttttatttaa cgattaatac agggatagtc gcacgttttg ctactttgtg gctgacactt
     4501 ccaagtacaa atttcttttt gtcttctgct ttacggttac ttaaaacaac aatttcgtat
     4561 ttaccgctat tagcatgttt taatagttct tcttttgcat taccacgaac aattatttgg
     4621 tcataatcaa taccataatc ttctaaagct ttgcgtgttt tttccaatcg cttgcttcgt
     4681 tcttcagtaa gtttgtttaa atgaacacct gctttaattg atgcttgagc atcttgttcg
     4741 ctgattgcgt ttaaaactgt tacgactgta ccttcgccag ctaatttaga cacttctttt
     4801 agtgcttttt cattttttaa ctgagtgtct acaccaagta atatattttt atacattagt
     4861 acatccctcc tcacttacat tctaataaat aataatggat ttttccattt aaaattaaag
     4921 attttgtgat ctttgtgttt gttgtatatt ttcaaaaaat aaaataggtg aaacgatcat
     4981 tatttcattt cacttcaatg ctttttatcc aaaataaagt tataatgtag ttaatactgt
     5041 tcgaaaagaa gaggtaaaga atgacaaaac atgaacaaat tttagattat attgaatcgt
     5101 tatctatagg ctctaaaatt tcggtgcgta aaatcgctaa atttttaaac gtatctgaag
     5161 ggacagcata tcgtgcaatt aaagatgctg ataaaatggg catggtcgca acgatagatc
     5221 gtgttggtac ggttcgaatc gaaaaacgta atcgtaatga aattgaacat ttaactttta
     5281 atgaaattgt caatattatt gatggccaag tgttaggtgg caacaaaggt atcactaaga
     5341 tggtatctaa atttgcaatt ggagcaatgg aactcaaaga tattttaaag tatattggtc
     5401 caaagacatt attgattgtc ggaaatcgtg aagatgtgca gattgaagct ttaaaaagag
     5461 gtacggctat attaattact ggtggattta agccatctaa taaagttatt aattttgcga
     5521 atgaacatga tttacctgtt ctatcatcaa gttatgatac attcctagtt gcaaatatta
     5581 tcaacaaagc actttttaat cagaaaatac gaaaagatat cttaatagtt caagatataa
     5641 tgacgccatt agatgattta tctgtgctgt ttgatacgat gaaaatagca gattataagc
     5701 gtatggcgaa tcgaactggc catacaagat ttccggtagt caatgagtcg tataaattag
     5761 taggtattgt aacaagtcga gagatgatta atacaaagga cgacgacgaa atagataaag
     5821 tgatgacacg aaatcctatt tatgtaaatg ctatgagtac agtagcaagt tgtgcgcata
     5881 tgatgatttg ggaaggtatt gaattgattc ctgtcgtttc gagtaataaa aagacagtgg
     5941 gtgtgattaa tagacaagat gttttaaagt ctatgcaact tttaggaaga cagcctcaga
     6001 tgggtgaaac aattaacgat caaatcgcca aatatataac aatgaatcaa gatggaatta
     6061 cagtagaagt ttcaccactt ttaatcaatc attacggaac agtaagtaaa gcggcatttg
     6121 taagtattat agaagaaaca atacaatatg aaatgcgtaa atttaaaaaa ggtaatgtca
     6181 tgatagagaa cttaaatatc gtgtatatta aaacagtacc tattgaatca catataacag
     6241 tgcgctttgg catactagac gtcggaagaa acttcgccaa aatagaagtg aacatgcata
     6301 gtcaaaacga caaagtagcg agcgctctcg tgatatgcca aatgtttgac gaagtatagg
     6361 aacttagcag agaaaatagg cataagatag atttcgcaca aacgttgggg ccccacccca
     6421 acttgcattg tttgtagaaa ttaggggtcc aatttctctg t
//
LOCUS       DAXWFK010000140         6457 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.141, whole genome shotgun sequence.
ACCESSION   DAXWFK010000140 DAXWFK010000000
VERSION     DAXWFK010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6457
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.141"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            17..568
                     /locus_tag="ACGEXS_002110"
     CDS             17..568
                     /locus_tag="ACGEXS_002110"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8013103.1"
                     /translation="MIRQARPEDRFDIAKLVYMVWDDMELELVKHLPKDMVLDAIEKS
                     CVDATYRTFYQHILVYEVENKVAGCIISYSGENELKYEKAWELLDLPEKIKQYGTPLP
                     VKEAKDDEYYIETIATFAAYRGRGIATKLLTSLLESNTHVKWSLNCDINNEAALKLYK
                     KVGFISDGQIELYKHMYHHLIVK"
     gene            complement(620..1558)
                     /locus_tag="ACGEXS_002111"
     CDS             complement(620..1558)
                     /locus_tag="ACGEXS_002111"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFF8013104.1"
                     /translation="MSNQYQQYSTVKKYWHLMRPHTLTASVVPVLVGTAASKIYFLGS
                     EDHIKISLFIAMLLACLLIQAATNMFNEYYDYKKGLDDHESVGIGGAIVRNGMSPELV
                     LRLAIAFYILAAILGLFLAANSSFWLLPVGLVCMAVGYLYTGGPFPISWTPFGELFSG
                     VFMGMFIIVIAFFIQTGNIQSYVIWLSVPIVITIGLINMANNIRDRVKDKASGRKTLP
                     ILLGKNASLTFMAIMYFIAYAFIVLTIIIKPGGSLFYLLALLSFPMPVKVIRRFKKND
                     TPPTMMPAMAAAGKTNTFFGLLYALGIYISALFAGI"
     gene            1709..1813
                     /locus_tag="ACGEXS_002112"
     CDS             1709..1813
                     /locus_tag="ACGEXS_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013105.1"
                     /translation="MKKDEERSEMDGYGRIRGRYCQRDIWQLKGMGFS"
     gene            1779..3101
                     /locus_tag="ACGEXS_002113"
     CDS             1779..3101
                     /locus_tag="ACGEXS_002113"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002499357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HFF8013106.1"
                     /translation="MYGSSKEWVSVEVKLSQSLDPSTLFHLTDNEAGDRFYMRLNDNR
                     TSYFGYKAIQLFKNNSKNKQSIFKDWEKLKHNITFIHPQSEKHHLRVVGGFQFSSHKS
                     DDEWREFGLNHFVLPEVLISTDNNGTFLTYTVKRESFTVEALNDLMDLFNNISDIDVD
                     EQIGEITRNEDIYKDDWRQLVVEAIESINNEEKIVLARRRLIKFDKDISIPYILKQAY
                     SKEKNSYIFLLESQDSIFFSQTPEQLIKVNNKILSTKAVAGTIKRSQDEDEDTKNVEA
                     FLKDNKNLIEHRFVVDSILHDIKPYITELHYDKTPKILKNDHLYHLYTEIKAPLKDDS
                     YISLIDNLHPTPALGGYPKEFAMDFIEQKEFGTRGLYGAPVGYIDIYDDCEFIVAIRS
                     MLIKKAQATLFAGCGIVKDSDPDSELAETNLKFTPMMNALGVDMNGKS"
     gene            3088..4761
                     /gene="menD"
                     /locus_tag="ACGEXS_002114"
     CDS             3088..4761
                     /gene="menD"
                     /locus_tag="ACGEXS_002114"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFF8013107.1"
                     /translation="MGNHKAALTKQVFTFASELYAYGVREVVISPGSRSTPLALAFEA
                     HPNIKTWIHPDERSAAFFAVGLIKGSERPVAILCTSGTAAANYTPAIAESQISRIPLI
                     VLTSDRPHELRSVGAPQAINQVNMFNNYVSYEFDMPIADDSKETIDAIYYQMQIASQY
                     LYGPHKGPIHFNLPFRDPLTPDLNATELLTSEMKILPHYQKSIDASALRHILNKKKGL
                     IIVGDMQHQEVDQILTYSTIYDLPILADPLSHLRKFDHPNVICTYDLLFRSGLDLNVD
                     FVIRVGKPVISKKLNQWLKKTDAFQILVQNNDKIDVFPIAPDISYEISANDFFRSLME
                     DTTVNRVSWLEKWQCLEKKGRKEIKCYLEQATDESAFVGELIKKTSEKDALFISNSMP
                     IRDVDNLLLNKNIDVYANRGANGIDGIVSTALGMAVHKRITLLIGDLSFYHDMNGLLM
                     SKLNNIQMNIVLLNNDGGGIFSYLPQKESATDYFERLFGTPTGLDFEYTAKLYQFDFK
                     RFNSVSEFKNATLLSETSTIYELITNREDNFKQHQILYQKLSEMIHDTL"
     gene            4748..5551
                     /gene="menH"
                     /locus_tag="ACGEXS_002115"
     CDS             4748..5551
                     /gene="menH"
                     /locus_tag="ACGEXS_002115"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFF8013108.1"
                     /translation="MTHYKFYEANVETNQVLVFLHGFLSDSRTYHNHIEKFTDNYHVI
                     TIDLPGHGEDQSSMDETWNFDYITTLLDRILDKYKDKSITLFGYSMGGRVALYYAING
                     HIPISNLILESTSPGIKEEANQLERRLVDDARAKVLDIAGIELFVNDWEKLPLFQSQL
                     ELPVEIQHQIRQQRLSQSPHKMAKALRDYGTGQMPNLWPRLKEIKVPTLILAGEYDEK
                     FVQIAKKMANLIPNSKCKLISATGHTIHVEDSDEFDTMILGFLKEEQND"
     gene            5544..6365
                     /gene="menB"
                     /locus_tag="ACGEXS_002116"
     CDS             5544..6365
                     /gene="menB"
                     /locus_tag="ACGEXS_002116"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFF8013109.1"
                     /translation="MTNRQWETLREYDEIKYEFYEGIAKVTINRPEVRNAFTPKTVAE
                     MIDAFSRARDDQNVSVIVLTGEGDLAFCSGGDQKKRGHGGYVGEDQIPRLNVLDLQRL
                     IRIIPKPVIAMVKGYAVGGGNVLNVVCDLTIAADNAIFGQTGPKVGSFDAGYGSGYLA
                     RIVGHKKAREIWYLCRQYNAQEALDMGLVNTVVPLEKVEDETVQWCKEIMKHSPTALR
                     FLKAAMNADTDGLAGLQQMAGDATLLYYTTDEAKEGRDAFKEKRDPDFDQFPKFP"
BASE COUNT         2324 a          938 c         1240 g         1955 t
ORIGIN      
        1 agggaatgat gacatagtga tacgtcaagc acgtccagag gaccgatttg atattgcgaa
       61 gttagtttat atggtttggg atgatatgga attagaattg gtaaagcatc tacctaaaga
      121 catggtatta gatgcaattg aaaaaagctg tgttgatgca acatatcgaa ctttttatca
      181 gcatatttta gtttatgaag tagaaaataa agtagcaggt tgtattatta gctatagtgg
      241 tgaaaatgaa ttgaaatacg aaaaagcatg ggaactactt gacttgccag aaaaaataaa
      301 acaatatggc acgccattac ctgtaaaaga agctaaagac gatgagtatt atatagaaac
      361 aattgcgaca tttgcagcat atagaggtag aggcatcgcg acaaagttat taacgtcatt
      421 acttgaatca aatacacatg ttaaatggag tttgaattgc gatattaata atgaagcagc
      481 attaaagtta tataaaaaag taggctttat atctgatgga cagattgaat tatacaagca
      541 catgtatcat catttaattg ttaaataaaa aaactcgaca gttcgatgta agtcgattgc
      601 cgagtagtat cattttctat taaatgcctg caaataatgc actaatataa atacctaatg
      661 catataataa accgaaaaat gtatttgttt taccagcagc agccattgct ggcatcattg
      721 taggcggtgt atcattcttc ttgaaacgtc tgataacttt aacaggcatt gggaatgata
      781 acaacgcaag taagtaaaat aatgagccac caggtttaat aatgatcgta agtacaataa
      841 aggcataagc gataaagtac atgattgcca taaatgttaa agaagcattt ttacctaata
      901 gaatgggtaa agttttgcga ccacttgctt tatctttgac acggtcgcga atattgttag
      961 ccatattaat taaaccgata gtgattacta taggtacact taaccaaatt acataacttt
     1021 gaatattgcc agtttgaata aagaatgcaa taacgataat aaacataccc ataaatacgc
     1081 ctgagaataa ttcaccgaaa ggcgtccatg aaatagggaa agggccacct gtatataggt
     1141 aaccaacagc catacatact aatccaactg gtaataacca aaatgaagag ttagcagcta
     1201 aaaacaaacc taatattgct gctaagatgt aaaatgcaat ggctaatcgt agcacaagct
     1261 ctgggctcat accgttgcga acaatggcac caccaatgcc tacagattca tgatcatcaa
     1321 ggcctttttt ataatcatag tattcattaa acatattagt tgctgcttga ataagtaagc
     1381 atgctagtaa catggcaatg aataggctga ttttaatatg atcttcgcta ccaagaaaat
     1441 atattttaga tgctgctgta ccaactaaaa cgggtactac ggaagcagtt aatgtatgag
     1501 gacgcattaa atgccaatat ttcttaactg tagaatattg ctgatattga ttactcattg
     1561 aatacaaaac ctctttaaat catatttttt ataacaaaga tattttaata gaaagtagca
     1621 taaaggtcaa ttgacgtgaa aaattgcaag ataagtttaa gcaattcttg taacacaatt
     1681 atccaaaaat gatacaatat aataatgagt gaaaaaagac gaagaaagaa gtgaaatgga
     1741 tggctacggg cgtattagag gacgatattg tcaaagagat atatggcagc tcaaaggaat
     1801 gggtttcagt tgaagtgaaa ttatcacagt cactagaccc gagcacatta tttcatctca
     1861 ctgacaatga ggcaggagat cgcttttata tgcgtttgaa tgataatcga acgtcatatt
     1921 ttggctacaa agcaattcaa ttattcaaaa ataattctaa aaataaacaa tctattttta
     1981 aagactggga aaaattaaaa cataacatca catttataca tccgcaatct gagaaacatc
     2041 atcttcgagt tgttggaggg ttccaatttt caagtcataa atcagatgat gaatggcgag
     2101 agtttggact aaatcatttt gtattacctg aagttttaat ttcaactgat aataatggga
     2161 catttttaac ttatacagtt aaaagggaaa gttttactgt tgaggcattg aacgatttaa
     2221 tggatttgtt caacaatata tcggacatag atgtggacga gcaaattggg gaaattacta
     2281 gaaatgaaga tatttataaa gatgactggc gtcaacttgt agtagaagct atagaatcta
     2341 ttaataatga agaaaaaatt gtactagcac gtagacggtt aataaagttc gataaagata
     2401 tcagtattcc atatattcta aagcaagcat attctaaaga aaaaaacagt tatatattct
     2461 tgttagaatc acaagattct atattctttt cacaaacacc tgaacaatta ataaaggtca
     2521 ataataaaat actatcgact aaagctgtag caggtacaat taaacgttca caagatgagg
     2581 acgaagatac aaaaaatgtt gaagcatttt taaaagataa taaaaactta atcgaacatc
     2641 gatttgttgt tgacagtatt ttacatgata ttaaacctta tatcactgaa ttacattatg
     2701 ataagacgcc taaaattcta aaaaatgatc atttatatca cttgtacact gaaataaagg
     2761 cgccactgaa ggatgattcg tatattagtt taattgataa tttacatcca acacctgctt
     2821 taggtggcta tccaaaagaa tttgcaatgg attttattga gcagaaagaa tttggtacgc
     2881 gaggattata tggtgcaccg gttggctata tagatatata cgatgattgt gaatttattg
     2941 ttgcaattcg ttcgatgctt attaagaaag cacaagcaac tttatttgct gggtgtggca
     3001 ttgttaaaga ttctgatcca gatagtgaat tggcagaaac gaaccttaag ttcacaccta
     3061 tgatgaatgc attaggagtc gatatgaatg ggaaatcata aagcagcttt aacgaagcaa
     3121 gtttttacat ttgcatctga gttatatgcg tacggcgtaa gggaagtagt tatcagtccg
     3181 ggatcacgct caacgccact tgcacttgca tttgaagcac atccaaatat taaaacatgg
     3241 atacaccccg atgagcgaag tgcagcgttt tttgcagttg ggttaattaa aggcagtgaa
     3301 agacctgtcg ctatattatg tacgtcaggt acagcagcag cgaattatac gcctgcaatt
     3361 gctgaaagcc aaattagtag aattccttta atcgttttaa caagtgaccg tccgcatgaa
     3421 ttaagaagtg taggcgcacc acaagcgatt aatcaagtaa atatgtttaa taattatgta
     3481 agttatgagt tcgatatgcc tattgcggat gatagtaaag agaccattga tgcaatttac
     3541 tatcaaatgc aaattgctag tcaatattta tatggaccac ataaagggcc aattcatttt
     3601 aacttgccat ttagagatcc gttaacacct gatttgaatg caacagaatt gttaacttct
     3661 gagatgaaga ttttaccgca ctatcaaaaa agtatagatg catcggcatt aagacacatt
     3721 ttaaataaga aaaaaggttt aattattgta ggggatatgc agcaccaaga agttgatcaa
     3781 atactaacgt attcaacgat atatgatttg cctattttag ctgatccttt aagtcattta
     3841 agaaaatttg atcatccgaa tgttatctgt acatatgatt tgctgtttag aagcggctta
     3901 gacttaaatg tggatttcgt aattcgtgtt gggaaaccag tgatttctaa aaagttaaat
     3961 caatggttaa agaaaactga tgcatttcaa atattagtgc aaaacaatga taagattgat
     4021 gtctttccga tagcgccaga tatttcatat gagatttctg cgaatgattt ctttaggtca
     4081 ttaatggaag acacgacagt taatcgcgta agttggttag aaaaatggca atgcttagag
     4141 aaaaaagggc gtaaagaaat taaatgttat ttggaacaag ctacagatga gagtgcattc
     4201 gttggtgaat tgattaagaa aacatctgaa aaagatgcat tatttattag taatagtatg
     4261 cctatcagag atgtagataa cttgttattg aataaaaata tagatgtcta tgcgaatcgt
     4321 ggtgcgaatg gtattgatgg tatcgtttca actgcactgg gtatggctgt gcataaacga
     4381 ataacattat tgataggtga tttatcattt tatcatgata tgaatggact attaatgtca
     4441 aaattaaata atattcagat gaatattgta ttattgaaca acgatggtgg cggtattttt
     4501 tcatatttac cacaaaaaga aagtgcaact gactattttg aacggttgtt tggcacaccg
     4561 acgggattgg atttcgagta tacagctaag ttatatcaat tcgattttaa acgttttaac
     4621 agtgtttcag aatttaaaaa tgccacattg ttatctgaaa cttcgacgat ttatgaattg
     4681 ataacgaatc gcgaagataa ctttaaacag catcaaattt tataccagaa attgagtgaa
     4741 atgattcatg acacattata aattttatga agcaaacgtt gagaccaatc aagttttagt
     4801 atttctgcat ggatttctta gcgacagccg tacttatcat aatcacatcg aaaaatttac
     4861 tgataactat catgtcatca ctatagactt accaggccat ggcgaagatc agtcttcaat
     4921 ggatgaaacg tggaattttg attatattac gacgttgtta gaccgaattt tagataaata
     4981 taaagataaa tcaataacat tgtttggata ttcaatgggt gggcgtgttg cattatatta
     5041 tgcaattaat ggtcacatcc ctatatctaa tttgatatta gaaagtacgt caccaggtat
     5101 taaagaagaa gcaaatcaat tggaacgccg tcttgttgat gatgcacgtg ctaaagtatt
     5161 agacatagca ggtattgaat tatttgttaa tgattgggaa aagttgccat tatttcaatc
     5221 gcaactagaa ttaccagttg aaatacaaca tcaaataaga caacaacgat tgtctcaatc
     5281 gccacataaa atggccaaag cattaagaga ttatggtaca ggtcaaatgc caaacttatg
     5341 gccgcgcctg aaagaaatta aagtaccaac attaatatta gctggagaat atgatgaaaa
     5401 atttgtacag attgcgaaaa aaatggcaaa tttaattcct aatagtaaat gtaaattaat
     5461 ttctgctaca ggtcatacaa ttcatgtgga agatagtgat gaatttgata caatgatatt
     5521 aggattttta aaggaggagc aaaatgacta acagacaatg ggaaacactt agagaatatg
     5581 atgaaatcaa atatgaattt tacgaaggga ttgctaaggt aacaataaat cgccctgaag
     5641 tacgcaatgc gtttacacct aaaacagttg ctgaaatgat tgacgcattt tcacgtgcac
     5701 gtgatgatca aaacgtttca gttatcgtat taactggtga aggtgattta gcattctgtt
     5761 ctggtggtga ccagaagaaa cgtggacatg gtggttatgt aggtgaagac caaatccctc
     5821 gcttaaatgt attagattta cagcgtttaa ttcgtattat tccaaaaccg gttatcgcga
     5881 tggtaaaagg ttatgctgta ggtggcggta atgtactaaa tgttgtttgt gacttaacga
     5941 ttgctgctga taatgctatt tttggacaaa ctggtcctaa agtaggttca tttgatgcag
     6001 gttatggttc aggatattta gcacgtatcg ttggacataa gaaagcacgt gaaatttggt
     6061 acttatgtcg tcaatacaat gcacaagaag ctttagatat gggtctagta aatacagtgg
     6121 tacctttaga gaaagttgaa gatgaaactg tgcaatggtg taaagagatt atgaaacact
     6181 caccaacagc gttacgattc cttaaagcag ctatgaatgc tgacacagat ggtttagctg
     6241 gtttacaaca aatggctggg gatgcaacat tgctttatta cacaactgat gaagcgaaag
     6301 aaggccgtga tgcgtttaaa gaaaaacgtg atcctgactt cgatcaattc cctaaattcc
     6361 cataagttat tttgaatatg atattaagtc acttgcgtcg tttattagcg acgataggtg
     6421 gcttttttat tttataagaa tttaaaaggg catttta
//
LOCUS       DAXWFK010000141         6416 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.142, whole genome shotgun sequence.
ACCESSION   DAXWFK010000141 DAXWFK010000000
VERSION     DAXWFK010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6416
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.142"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..302)
                     /locus_tag="ACGEXS_002117"
     CDS             complement(<1..302)
                     /locus_tag="ACGEXS_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFF8013110.1"
                     /translation="MIKLNFNDLEIFITVCEEASINKAAIKLRYAQSNISQRISKLEN
                     ELGVVLLFRNQKGAKATKAGEEFLAYSKKVLRDTETIKNKMKNNTMSILCSELLFN"
     gene            393..926
                     /locus_tag="ACGEXS_002118"
     CDS             393..926
                     /locus_tag="ACGEXS_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4865 family protein"
                     /protein_id="HFF8013111.1"
                     /translation="MHAMQYHVKLPSDYNMEIIRDRVRLNGYKTDGFKNLIIKAYLIS
                     QTTTNCITNTYSPLYLWRSSEGMTEFIFNGFYDNVISSFGWQNINIGVIYSMNITDSV
                     KHSLYALEEYIDIFPTLSLKEIEIKKLFRIFDNAVAEIIIYNPDKWKFVKYSFIENLP
                     KSLNPNIKIYDVLYISQ"
     gene            complement(1281..2141)
                     /locus_tag="ACGEXS_002119"
     CDS             complement(1281..2141)
                     /locus_tag="ACGEXS_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFF8013112.1"
                     /translation="MNLNILKSFLVTSETKNLTKASELLNYSQSTVSTHIEKLERQLD
                     VKLFYRKKYGMELTEEGLAYVKYAKVILDSNSEYEREIKGLYNKKVNISINMQESQYL
                     YRYYNKISEWLAEHPYVNLKFKSAHSNFYIKEEIANFKSDISLITDEKIINSNLTAIP
                     ITEERLIFVTNKEMKDFKLNDIQNHTLLVTEKGCSYREQLEMILYKYSFSTKQMIEFL
                     GIESLKKHLKNSGGIALLPEFIVADELENKSLFQIDVDVDIPNLETTLIINPESNKQV
                     LESFVKDVFL"
     gene            2254..2805
                     /locus_tag="ACGEXS_002120"
     CDS             2254..2805
                     /locus_tag="ACGEXS_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFF8013113.1"
                     /translation="MITCLFGSSRKDGNSAIAVENLLKNLKVNFMNLYQSNIEKVTDN
                     RHSKGANFHNDDYEQILIKVLESDIIIFSTPLYWYSMSASLKLFIDRWTESLRDTQID
                     NFKEIMSQKKYLILIIGGDNPHTKAQPLVNQFKLIFEFMNITNYRFLIGEGNKPFDIL
                     NDSQFIEELANINLALKKGDIYD"
     gene            2798..4018
                     /locus_tag="ACGEXS_002121"
     CDS             2798..4018
                     /locus_tag="ACGEXS_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8013114.1"
                     /translation="MTDLLKIKNFRLFFLAEIISAFGVGISTVGANWYLIDKTNDSQL
                     LGIMLALNVLSGFLASPIIGGLADKYNRRNIILITYLLQVILYLLIVIALVMIGFETY
                     LVIGFAIVNGIGWTTYMATSRSLVKQILKPDQYTDANSLLEISLQTGMFIAGGLSGIL
                     YKINGFTLIIAMTIMMFLISIFMLFRLHVDKPTHSEEESTNSLLQEYLLGWKFLKDNM
                     MIFIFGVISIIPMVFTMIFNISLPGYVYNVLKLSSVQFGFSDMLYGIGGLCAGLISAI
                     LSKKISTKVLIFLLYFILVINSALFIWINSAFYLFIGSFILGYSISSIRIYMNTAIMN
                     TVSDKYVGRSFTIWTSISLLLQSLIAPFLGRWINEINDKFGFYIILILSLLIFVTLLL
                     VNKTDKIKYAHKEE"
     gene            complement(4090..4386)
                     /locus_tag="ACGEXS_002122"
     CDS             complement(4090..4386)
                     /locus_tag="ACGEXS_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HFF8013115.1"
                     /translation="MATKKDVHDLFLNHVNSNAVKTRKMMGEYIIYYDGVVIGGLYDN
                     RLLVKATKSAQQKLQDNTLVSPYPGSKEMILILDFTEATNLTDLFKTIKNDLKK"
     gene            complement(4435..4638)
                     /locus_tag="ACGEXS_002123"
     CDS             complement(4435..4638)
                     /locus_tag="ACGEXS_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6007 family protein"
                     /protein_id="HFF8013116.1"
                     /translation="MEGLKHSLKSLGWWDLFFAIPIFLLFAYLPNYNFITIFLNIVII
                     IFFSIGLILTTHIIIDKIKSNTK"
     gene            4947..5933
                     /locus_tag="ACGEXS_002124"
     CDS             4947..5933
                     /locus_tag="ACGEXS_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFF8013117.1"
                     /translation="MKENFWSELPRPFFILAPMEDVTDIVFRHVVSEAARPDVFFTEF
                     TNTESFCHPEGIHSVRGRLTFSEDEQPMVAHIWGDKPEQFRETSIQLAKMGFKGIDLN
                     MGCPVANVAKKGKGSGLILRPDVAAEIIQATKAGGLPVSVKTRLGYYEIDEWKDWLKH
                     VFEQDIANLSIHLRTRKEMSKVDAHWELIEAIKNLRDEIAPNTLLTINGDIPDRKTGL
                     ELAEKYGIDGVMIGRGIFHNPFAFEKEPREHTSKELLDLLRLHLSLFNKYEKDEIRQF
                     KSLRRFFKIYVRGIRGASELRHQLMNTQSIAEARALLDEFEAQMDEDVKIEL"
     gene            6119..6333
                     /locus_tag="ACGEXS_002125"
                     /pseudo
     CDS             6119..6333
                     /locus_tag="ACGEXS_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282781.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
BASE COUNT         2219 a          967 c          953 g         2277 t
ORIGIN      
        1 taattaaata acagttctga gcataaaata gacatagtat tatttttcat tttatttttt
       61 atagtctctg tatctcttaa tacttttttg ctatacgcta agaattcttc gcctgcttta
      121 gttgccttag cacctttttg atttctaaaa agcaaaacta cacctaattc attttcaagc
      181 ttgctaattc tttgagatat attagattgt gcatatctaa gtttaattgc agctttattg
      241 atagatgctt cttcacatac agttataaaa atttccaaat cattaaagtt caatttaatc
      301 acctatcact ttttatgata actatatcac aattggataa tatgagattt tacttattat
      361 tgctatcctt tttataaaag ggggtatgct aaatgcatgc aatgcaatat catgttaagt
      421 tgcctagtga ttacaatatg gaaataatta gagatagagt tcgattaaat ggttataaga
      481 ctgatggatt taaaaattta attataaaag cttatttaat aagtcaaaca actactaatt
      541 gcattacaaa tacttattca ccattatatt tatggagaag ttcagagggg atgactgaat
      601 ttatattcaa tggattttat gataatgtta tttcttcttt tggatggcaa aatataaata
      661 ttggtgtgat atattcaatg aacataaccg attctgttaa acatagtctt tatgcgcttg
      721 aagaatatat cgacattttc cctactttat ctctaaaaga aatagaaatc aaaaaattgt
      781 ttagaatctt cgataatgca gttgcagaaa ttataattta taatcctgat aagtggaagt
      841 ttgtgaaata ttcatttatt gaaaatcttc caaagagtct caatccaaat ataaaaattt
      901 atgatgtact atatatctca caatgaaagt acagatttaa agttattatt atcaacatta
      961 gaaaacagac taaaataatt tatctgaact tattaataat aaagaacctg ctagatgaca
     1021 gctatttaaa atacataagc tactcattta gcaggttaaa atattaatca atccatacta
     1081 actagtatga ttcaaaggtt ttcatctatt aaattcttta ctattttgaa aatgaatgcc
     1141 cgcaatttga aatttcaact agaaataatc cttattaaag atataaatca cctgttttta
     1201 tattgaatta ctttttcatt aacatctcat ttaaattatt atctttgagt ttcaccataa
     1261 ctacacgttt tcaccaataa ttataaaaaa acatctttta caaaagattc aagtacttgc
     1321 ttattcgatt caggattaat tatcaatgtt gtttctaaat tcggaatatc gacatctacg
     1381 tctatttgaa acaaactttt attttctaat tcatctgcaa caataaattc cggcaatagc
     1441 gcaatcccac ctgaattttt caagtgtttt tttaatgact caattcctag aaattcaatc
     1501 atttgttttg ttgaaaagct atatttatat aaaatcattt ctaactgttc tctgtaacta
     1561 cacccttttt cagtaacaag taatgtatgg ttttgaatat cgtttaattt gaaatctttc
     1621 atctctttgt tagtaacaaa tattaaacgt tcttcagtta taggaatagc agttaagtta
     1681 ctattaataa tcttttcgtc tgtgataagg ctaatatccg atttgaaatt agcaatttct
     1741 tctttaatat agaaattaga atgtgcggat ttaaacttca agtttacata tgggtgttca
     1801 gctaaccatt cactaatctt attatagtag cgatacaaat actgactttc ttgcatgtta
     1861 atacttatat ttaccttctt attgtaaagt ccttttattt ctctctcgta ttcgctatta
     1921 ctatctaaaa tcactttagc gtatttaacg tatgctaagc cttcttccgt tagttccata
     1981 ccatattttt ttctataaaa taatttaaca tctaattgcc tttctaattt ttcaatatgt
     2041 gtagatacag ttgactgtga atagttaagt aactctgatg ctttagtaag gttctttgtt
     2101 tcactagtta ctaaaaatga ttttaaaatg tttaagttca aagtagtacc tcctttaggg
     2161 ttccctttta tcgaatcctg aattctaaga attctgattt aataataata tattaaatat
     2221 tattataaag gtaaatcgaa aggagttatg actatgatta cttgtttatt tggaagtagt
     2281 agaaaagatg gcaattcagc tatcgcagta gagaatctat tgaagaattt aaaagtcaac
     2341 tttatgaatt tatatcaaag caacattgaa aaggttacag ataatcgtca tagcaaaggt
     2401 gccaactttc ataatgatga ttacgaacaa attttaatca aagttttaga atcagatatt
     2461 attatttttt caacaccact ctattggtac tccatgagtg cgtctttaaa actcttcatt
     2521 gatagatgga ccgagtcatt gcgagatacg caaatagaca acttcaaaga aatcatgtca
     2581 caaaaaaagt atttgatttt aatcataggt ggcgacaatc cgcataccaa agcacaacct
     2641 ttagtcaacc aattcaaatt aatatttgag tttatgaata ttactaacta tagatttttg
     2701 atcggcgaag gcaataaacc attcgatatt ttaaacgact ctcaatttat agaagagctc
     2761 gcaaatataa atcttgcatt gaaaaaaggt gatatttatg actgatttgt tgaaaattaa
     2821 aaacttcaga ttgttctttt tagcagaaat aatttcagca ttcggagttg gaattagtac
     2881 agttggggct aattggtatt taatcgacaa aactaacgac tcccaactat taggtattat
     2941 gttagcttta aatgtattat ccggtttctt agcttctcct ataataggtg gactagccga
     3001 taaatataat agacgaaata ttattttaat cacctatctt cttcaggtaa tattatattt
     3061 actcattgtc atagcactcg taatgatagg atttgagacc tatttagtga ttggatttgc
     3121 gattgttaat gggataggtt ggactacgta tatggcaaca tctagaagtt tagttaaaca
     3181 gatattaaaa ccagatcaat atacagatgc aaattctcta ttagagatta gtttgcaaac
     3241 aggtatgttt attgcaggtg gattatcagg aatattatat aaaataaatg gattcactct
     3301 aattatagcg atgactataa tgatgtttct aatcagcatt tttatgttat ttagattgca
     3361 tgtagataaa ccaactcatt cagaggaaga atcaacaaat agcttattgc aagaatattt
     3421 attaggatgg aaatttttaa aagacaacat gatgatattt atttttggag ttatttcaat
     3481 cataccaatg gtgtttacaa tgatctttaa catatcatta ccaggatatg tttataatgt
     3541 tttaaaactt tcgtctgtac aatttggttt ttcagatatg ttatatggca ttggaggatt
     3601 atgtgcaggt ttaatttcag ctattctttc gaagaaaatc tcaactaaag tattgatatt
     3661 tttgttatat ttcatattag tcataaattc agcactattt atttggataa actcagcatt
     3721 ttacttattc ataggatcat ttatactagg ctactcaatt tcatcaatca gaatttatat
     3781 gaatacagct ataatgaaca ctgtttcaga taaatatgtc ggtcgctcat ttacgatatg
     3841 gacgtcaatt tcattgttac tgcaaagttt aattgctcca tttttaggaa gatggattaa
     3901 tgaaattaat gataaattcg gtttctatat tatactcatt ttatccttac tcatatttgt
     3961 tacactgctg cttgttaaca aaacagacaa aataaaatat gcacataaag aagagtgacc
     4021 gtcactcttc tttaacaagc gaccatttat cgatgggctt agttctctct gcacccacac
     4081 ttcactactt cactttttca aatcattttt tatggtctta aataaatcag tgagatttgt
     4141 tgcttcggta aagtctagaa ttaatatcat ttctttagaa cctggatatg gcgaaactaa
     4201 tgtattatct tgcaatttct gctgggcact tttagtcgcc ttgaccaata atctattatc
     4261 atacaaacca cctataacca cgccatcata ataaataata tattctccca tcatctttct
     4321 tgtcttaacc gcgtttgaat tcacatgatt taaaaataaa tcatgtacat ctttcttagt
     4381 cgccattgta atcgctcctt cagttttatg tttaatcaca ttcgtattaa tgattcattt
     4441 cgtgttgctc ttaattttat ctataattat atgcgtagtt aaaatcaaac ctatggaaaa
     4501 gaaaataatg ataacaatgt taagaaatat agttataaaa ttatagtttg gaaggtatgc
     4561 gaatagcaga aaaataggta tcgcaaaaaa taaatcccac caacctaaac tttttaaaga
     4621 atgctttaaa ccttccataa tatcaccttt ataaatttgt ctttgttata agataactaa
     4681 aaaatcgctt tactgtaaaa gtagccaaag aaaattctga atcatattca taagtagtgt
     4741 atcattaata atgaacaatt taatactata atccttgatc tttgtattga tcaacttacc
     4801 acaacattta ttttagacta ctcttagact tccctttcaa atggttgcat ctattgaaat
     4861 tccttttgta taagttaggc ttttgtggta atatcatcat gcataaaaaa tcgagatact
     4921 aattataaag agggtataaa tatattatga aagaaaattt ttggagtgaa ttaccacgtc
     4981 cattttttat tttggcgcca atggaagacg ttacagatat cgtctttcga cacgttgtaa
     5041 gtgaagcagc tagaccggat gtgtttttca ctgaatttac aaatactgaa agcttttgcc
     5101 accctgaagg catacatagt gtgcgcggac gcttaacttt tagtgaagat gaacagccga
     5161 tggtcgctca tatatgggga gataagccag aacagttccg tgaaacgagt attcaattag
     5221 ctaaaatggg ctttaaaggc atagacttaa atatgggatg tcctgtagca aatgttgcta
     5281 aaaagggtaa gggttccggc ttaatcttaa gacctgacgt tgctgccgaa attattcaag
     5341 cgactaaagc aggtgggctt ccggtaagtg ttaaaacacg ccttggctac tatgaaatcg
     5401 atgaatggaa agattggttg aagcacgtct tcgaacaaga cattgccaat ttatctattc
     5461 atcttcgtac acgtaaagaa atgagtaaag tagatgcaca ttgggaatta atcgaagcta
     5521 ttaaaaattt acgtgacgaa attgcaccaa atacattgtt aacaattaac ggtgatattc
     5581 ccgatagaaa aacaggactt gaactggcag aaaaatatgg cattgatggc gtcatgattg
     5641 gtagaggcat tttccacaat ccattcgctt ttgaaaaaga accacgcgaa cacacaagca
     5701 aggaactatt agatctattg agattgcatt tatcattgtt taacaaatat gaaaaagatg
     5761 aaatacgaca attcaagagc ttgcgtagat tctttaaaat ctatgtgcgt ggcataagag
     5821 gcgctagcga acttcgacat caattgatga acacacaatc aattgcagaa gcacgagcac
     5881 tactcgatga atttgaagcc caaatggacg aagacgttaa aattgaatta tagtatgagt
     5941 aataaagttt atggatgata tttcccaatt taacacggat tgaacacttt ctacttaggt
     6001 attatcttgg ttttcctgat aggataactc ccgatgatgc ctttttactt atgtcactta
     6061 tccttttgga aatggtgcaa aattaaaaat acatcaggat atcgtttaac tcagaaaaaa
     6121 actccaatca aaaaaaatgt ttacaacaca ttatgaaatt aaaattgctc ttagtgttca
     6181 acagacaaag ttattgaaat tactattgga ggtacatacc aagctatcat tttgagtaca
     6241 cgtggtttga atatctggca agaaatggaa attggtaatg tcagtcataa agtcgctaaa
     6301 cgtgctcaaa taccattaat tatttttaaa taatttagag gatgaggtat aaacctttaa
     6361 aaaacagcag tgagatgatt ttcaattaga aaatatctta ctgctgtttc tatttt
//
LOCUS       DAXWFK010000142         6289 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.143, whole genome shotgun sequence.
ACCESSION   DAXWFK010000142 DAXWFK010000000
VERSION     DAXWFK010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6289
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.143"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..571
                     /locus_tag="ACGEXS_002126"
     CDS             <1..571
                     /locus_tag="ACGEXS_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFF8013118.1"
                     /translation="FRSFHAISSFKLENLCSLYDIDKEKLHSAKFDVLALSKMLISLA
                     KNNQHYSVLKLIHYMPKQYIRFSKYSNSPTKLFDSGFQKIHMKISEINKIEVESVIPI
                     LKDKNVVFTGNFDTEKQDLMILTRKKGAYIRSDVTAKTDILVEGVQDDKYKDVNGLVS
                     KQRKAREYVGNGAKIQFLNEEDLINLIKE"
     gene            577..918
                     /locus_tag="ACGEXS_002127"
     CDS             577..918
                     /locus_tag="ACGEXS_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013119.1"
                     /translation="MIKKIFTKKHVFLVIEDENHNHSDAVFGKSILLSIYVGVNKKTN
                     SKSGKFIYLDRSKRIVRQSDITKIESANENDVDFYNLLKKEKEIVYSKNIVDKYNLAN
                     YIIYYEVSTKE"
     gene            1122..1304
                     /locus_tag="ACGEXS_002128"
     CDS             1122..1304
                     /locus_tag="ACGEXS_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013120.1"
                     /translation="MKITNCKIKRETVIYEVLTSGNQPFTYELPKDLSSHNARKYLEF
                     ISQKLDGDKLTKEDSL"
     gene            1404..1868
                     /locus_tag="ACGEXS_002129"
     CDS             1404..1868
                     /locus_tag="ACGEXS_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013121.1"
                     /translation="MKRLLGLLLVSTLVLSACGNDENQEESKKEVKSKEKKIEKEKEN
                     KSKKDKEKEVATQQQPDNQTVEQPQSQEQSVQQPQQQIPQNSVPQQNVQVQQNKKQKV
                     DLNNMPPTDFSTEGMSEQAQKQIEELSMQKDYHGLSQREYNDRVSEIINNDN"
     gene            1927..2964
                     /locus_tag="ACGEXS_002130"
     CDS             1927..2964
                     /locus_tag="ACGEXS_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8013122.1"
                     /translation="MKTRCYDGKKWQYEFKHEGKRYRKKGFRTKREANSAGLDKLNEL
                     RSGFNIDNYITLEEYFENWIKTYKQPVVKENTYRHYRNALQHIQKHKIGKMELSKINR
                     QVYQKFINDYSKEHAKETIRKTNGAIRSALDDALYDGLIFKNPAYKVNYKAGKPTKSE
                     QEKFISVTEYEILKDHVRKKRTRSSLALFIMICTGCRVSGARNIKIEHINQVKNTIFI
                     DERKTNTSPRYISIAKSDMKHIMDVISTFAISYDGYIFKEGGSIINLHAINNALKSAC
                     RVNNIPIITSHALRHTHCSYLLAKGVSIHYISKRLGHKNIAITTSVYSHLLEEKFNEE
                     DKKTTKILESM"
     gene            3021..3845
                     /gene="sph"
                     /locus_tag="ACGEXS_002131"
                     /pseudo
     CDS             3021..3845
                     /gene="sph"
                     /locus_tag="ACGEXS_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239616.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004767 - sphingomyelin phosphodiesterase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sphingomyelin phosphodiesterase"
     gene            complement(4083..5099)
                     /gene="lukG"
                     /locus_tag="ACGEXS_002132"
     CDS             complement(4083..5099)
                     /gene="lukG"
                     /locus_tag="ACGEXS_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukGH subunit G"
                     /protein_id="HFF8013123.1"
                     /translation="MIKQLCKNITICTLALSTTFTVLPATSFAKINSEIKQVSEKNLD
                     GDTKMYTRTATTSDSQKNITQSLQFNFLTEPNYDKETVFIKAKGTIGSGLRILDPNGY
                     WNSTLRWPGSYSVSIQNVDDNNNTNVTDFAPKNQDESREVKYTYGYKTGGDFSINRGG
                     LTGNIIKESNYSETISYQQPSYRTLLDQSTSHKGVGWKVEAHLINNMGHDHTRQLTND
                     SDNRTKSEIFSLTRNGNLWAKDNFTPKDKMPVTVSEGFNPEFLAVMSHDKKDKGKSQF
                     VVHYKRSMDEFKIDWNRHGFWGYWSGENHVDKKEEKLSALYEVDWKTHNVKFVKVLND
                     NEKK"
     gene            complement(5121..6176)
                     /gene="lukH"
                     /locus_tag="ACGEXS_002133"
     CDS             complement(5121..6176)
                     /gene="lukH"
                     /locus_tag="ACGEXS_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukGH subunit H"
                     /protein_id="HFF8013124.1"
                     /translation="MKNKKRVLIASSLSCAILLLSAATTQANSAHKDSQDQNKKEHVD
                     KSQQKDKRNVTNKDKNSTAPDDIGKNGKITKRTETVYDEKTNILQNLQFDFIDDPTYD
                     KNVLLVKKQGSIHSNLKFESHKEEKNSNWLKYPSEYHVDFQVKRNRKTEILDQLPKNK
                     ISTAKVDSTFSYSSGGKFDSTKGIGRTSSNSYSKTISYNQQNYDTIASGKNNNWHVHW
                     SVIANDLKYGGEVKNRNDELLFYRNTRIATVENPELSFASKYRYPALVRSGFNPEFLT
                     YLSNEKSNEKTQFEVTYTRNQDILKNRPGIHYAPPILEKNKDGQRLIVTYEVDWKNKT
                     VKVVDKYSDDNKPYKEG"
BASE COUNT         2306 a          950 c          945 g         2088 t
ORIGIN      
        1 ctttagaagc ttccacgcaa tctctagttt taaattagaa aatttgtgta gcttatatga
       61 tatcgataaa gaaaaattac attctgctaa atttgacgtg ctagctttat cgaagatgtt
      121 gatatcactc gctaaaaaca atcagcatta tagtgtatta aaattaatac attatatgcc
      181 taagcaatac attagattta gcaaatattc taactctcca actaaacttt tcgattcagg
      241 atttcaaaaa attcatatga aaatatctga gattaataaa atagaagtgg aaagtgtaat
      301 ccctatttta aaagataaaa atgttgtttt tacaggtaat tttgacactg aaaaacaaga
      361 tttaatgata ttaactagaa agaaaggagc ttatatcaga agtgacgtaa ctgcaaaaac
      421 agatatttta gtcgaaggtg ttcaagatga taaatataaa gatgtgaacg gactagtttc
      481 aaaacaacga aaagctcgag aatatgttgg aaatggtgca aaaattcaat ttttaaatga
      541 agaagactta ataaatttaa taaaggaata ataacgatga tcaaaaaaat ttttacaaaa
      601 aagcatgtat tcttagttat agaagatgaa aaccataatc acagtgatgc tgtttttgga
      661 aaaagtatat tactttcaat ttacgtcggt gtgaataaaa agactaattc taaatcaggg
      721 aaatttatat accttgacag atctaaaaga atcgttagac aatctgatat caccaaaata
      781 gaatcagcta acgaaaatga tgtagatttt tataatttac tgaagaaaga aaaggaaatt
      841 gtttattcca aaaatatagt agataaatac aatttagcga actatataat ttactacgaa
      901 gttagtacta aagaataaac caatccatta tttcataata ctaaccttaa atttacagag
      961 gttttaatta tgaaacatga aaaaagcaat cttaacttta agtcttatat ttattaccta
     1021 ctacctcact tttaaatata tgtggattaa agaattgaag tattaattat gcttatttaa
     1081 aaaagacgtc tatttcagca gtgtttgaaa ggaagtttat aatgaaaata actaattgca
     1141 aaataaaaag agaaactgta atatacgaag ttttaactag tggtaatcaa ccattcactt
     1201 atgagttacc taaagattta tcgtcacata atgcgcgtaa atacttggaa tttatttcac
     1261 aaaaattaga tggcgataag ttaaccaaag aagattcatt atgattttac taaacaaaaa
     1321 aacgcctact agtgtgaaaa cgtattgatt aatagcgcct atatggagtt ttaatataaa
     1381 aagtaagcaa aggagaaatg agaatgaaaa gattgttagg tttattatta gtgagcacgt
     1441 tagtgttaag tgcatgtgga aatgatgaga atcaggaaga atctaaaaaa gaagttaaat
     1501 caaaagaaaa gaaaattgag aaggaaaagg aaaataaatc gaaaaaagat aaggaaaaag
     1561 aagttgcaac acaacaacaa ccagacaatc aaaccgttga acaaccccaa tcacaagagc
     1621 aatcggttca acaaccgcaa caacagatac cacaaaatag tgttcctcag caaaatgtcc
     1681 aagttcaaca aaacaaaaag caaaaagttg atttaaataa tatgcctccc actgattttt
     1741 ctacagaggg tatgtctgag caggctcaaa aacaaattga agagctttca atgcaaaaag
     1801 actatcatgg tctgtcacaa agagaataca atgatagagt ttctgaaatt ataaataatg
     1861 ataattgatt ccacagggta gcccgcctac ccttattatt ttttgccaat tttgaggagg
     1921 gaacgcatga aaacacgttg ttacgatggt aaaaaatggc aatatgaatt taagcatgaa
     1981 ggaaaaagat accgtaagaa aggttttaga acaaagcgtg aagctaattc tgctggacta
     2041 gacaagttaa atgagttaag aagtggtttt aatatagata actatataac tcttgaagaa
     2101 tacttcgaaa attggattaa aacatataaa caacctgttg ttaaagaaaa tacctaccgt
     2161 cattatagaa atgcattaca acatatacaa aaacataaaa taggtaaaat ggagttatca
     2221 aagataaata gacaagttta tcagaaattc ataaacgact attcaaaaga acacgcaaaa
     2281 gaaactataa gaaaaacaaa cggtgctatt cggtcagctt tagatgacgc attatatgat
     2341 ggacttattt ttaaaaaccc cgcttataaa gttaattata aagccggaaa acctacgaag
     2401 tcagaacaag aaaaattcat ctcggtaact gaatatgaaa tactaaaaga tcacgtcaga
     2461 aagaagagaa ctcgttcatc attagcgcta ttcataatga tttgtacggg ttgtcgtgtc
     2521 agtggtgcaa gaaatataaa gattgagcat atcaaccaag tgaaaaacac tatatttatt
     2581 gacgagcgaa aaaccaatac ttcccctaga tatatcagta tcgctaaatc tgatatgaaa
     2641 cacattatgg acgtcataag tacatttgca attagctatg atggttacat tttcaaagaa
     2701 ggcggatcta taattaacct tcatgctatc aataatgctt tgaaatcagc ctgtagagtc
     2761 aataatatac caattattac atcgcacgca ttaagacaca ctcattgttc ttatttacta
     2821 gcaaaaggtg tatctataca ttacatttct aaaagattag gtcataaaaa tatagcaata
     2881 actacatctg tgtattctca tttgttagaa gaaaaattta atgaagagga caaaaaaaca
     2941 actaaaattt tagaaagtat gtaatttagg gacccattag ggaatccaaa cccaataaat
     3001 actgttgtta caaggtttct atgtatccaa actgggggca atataaacgc gctgatttaa
     3061 tcggacaatc ttcttatatt aaaaataatg atgtcgtaat attcaatgaa gcatttgata
     3121 atggtgcatc agacaaatta ttaagtaatg tgaaaaaaga atatccttat caaacacctg
     3181 tactcggccg ttctcaatca gggtgggaca aaactgaagg tagctactca tcaactgttg
     3241 cagaagatgg tggcgtagcg attgtaagta aatatcctat taaagaaaaa atccagcatg
     3301 ttttcaaaag cggttgtgga ttcgataatg atagcaacaa aggctttgtt tatacaaaaa
     3361 tagagaaaaa tggtaagaac gttcacgtta tcggtacaca tacacaatct gaagattcac
     3421 gttgtggtgc tggacatgat cgaaaaatta gagctgaaca aatgaaagaa atcagtgact
     3481 ttgttaaaaa gaaaaatatc cctaaagatg aaacggtata tataggtggc gaccttaatg
     3541 tcaataaagg cactccagag ttcaaagata tgcttaaaaa cttgaatgta aatgatgttc
     3601 tatatgcagg tcataatagc acatgggacc ctcaatcaaa ttcaattgcg aaatataatt
     3661 accctaatgg taaaccagaa catttagact atatatttac agataaagat cataaacaac
     3721 caaaacaatt agtcaatgaa gttgtgactg aaaaacctaa gccatgggat gtatatgcgt
     3781 tcccatatta ctacgtttac aatgattttt cagatcatta cccaatcaaa gcctatagta
     3841 aatagtgctc aactaactaa taactcgctt cgttctaaaa ggacgaagcg agttatattg
     3901 ttaaaatttg aattgactta cattttaata aaatcatctt aacaacttta atttttcatt
     3961 aatacaagtc tttactctac actcaaacaa gattcataca ctgcacgtca taataaatct
     4021 atctattcaa atataaataa aagttaccta ctacattcta tgtagcaggc aacttttatt
     4081 acttatttct tttcattatc attaagtact tttacaaact tcacattatg tgtcttccaa
     4141 tcaacttcat ataatgctga taatttttct tcttttttat ctacatggtt ttcaccagac
     4201 caatagcccc agaaaccatg gcgattccaa tctattttaa actcatccat tgatctttta
     4261 taatgaacaa caaattgtga tttacctttg tcttttttat catgtgacat aacagctaaa
     4321 aattctggat taaacccttc agacacagtt acaggcattt tgtctttagg tgtgaaatta
     4381 tctttcgccc ataaatttcc atttcgtgtt aaagaaaaaa tttcactttt agttctatta
     4441 tcactatcat tagttaattg tctcgtatgg tcatgtccca tattatttat caaatgtgct
     4501 tctactttcc aacctacacc tttatgtgac gtagattgat caagtaatgt acgatatgat
     4561 ggttgttgat aactaatcgt ctctgaataa ttactctctt ttataatatt tccagttaag
     4621 cctccacgat taatcgaaaa atctccacct gttttataac catacgtata tttaacttct
     4681 cttgattcat cctgattttt tggtgcaaag tcagtcacat ttgtattgtt gttgtcatca
     4741 acattttgaa ttgaaactga ataagatcca ggccatctta atgtactatt ccaataacca
     4801 tttgggtcta aaattctcaa accactacca attgtacctt ttgctttaat aaatactgtt
     4861 tctttatcat aattaggttc agttaagaaa ttaaattgta agctttgagt aatatttttt
     4921 tgactatcac ttgttgtagc tgtacgtgta tacattttag tatcaccatc aagattcttc
     4981 tcagaaactt gtttgatttc agaattaatc tttgcaaatg aagtagctgg taatacagtg
     5041 aaagtagtcg atagtgctaa cgtacaaatt gtgatatttt tacatagttg tttaatcatt
     5101 agtaatccgc cctttcaata ttatccttct ttataaggtt tattgtcatc agaatattta
     5161 tcaacgactt taactgtttt atttttccaa tcaacttcat aagtgacaat taatctttga
     5221 ccatctttat ttttttctaa aattggaggt gcataatgta ttccaggtct gtttttcaaa
     5281 atatcttgat ttcgtgtgta tgttacttca aattgcgttt tctcatttga cttttcatta
     5341 gataaataag ttaaaaattc tggattaaag ccacttctta ctaatgctgg gtatctatat
     5401 tttgaagcaa agcttagttc agggttttct acagtagcaa ttctcgtatt tctatagaat
     5461 aataattcat catttctatt tttcacttct ccaccatact tcaagtcatt cgcaataact
     5521 gaccagtgta catgccagtt attattttta ccgctggcaa ttgtgtcata attttgctga
     5581 ttataactaa tcgttttgga gtagctattt gatgaagttc gtccaatacc ttttgttgaa
     5641 tcgaatttac cacctgagct atatgaaaat gtactgtcta cttttgcagt tgaaatttta
     5701 tttttcggca attggtctaa tatttcagtt ttacgatttc tttttacttg aaaatctaca
     5761 tggtactcac ttggatactt taaccaattt gaattttttt cttctttatg agattcaaac
     5821 tttaaatttg aatgaattga gccttgtttt ttaacaagta atacattctt gtcataagtt
     5881 ggatcatcga taaagtcgaa ttgtaaattt tggagtatat ttgttttctc atcatatact
     5941 gtttcagttc gttttgtgat tttaccgttt ttcccaatat catccggtgc tgttgaattt
     6001 ttatctttat tagtaacatt acgtttgtct ttttgttgag acttatcaac atgttctttc
     6061 ttattttggt cttgagagtc tttatgagct gaatttgctt gagtcgttgc tgctgataac
     6121 aataaaattg cacatgataa tgatgacgct attaaaacac gttttttatt tttcattgtt
     6181 ataaccttct ttcgtatgat tgatatttgt tgatatgtat cgacatgtga ataataacac
     6241 aaaaacagag aatatatatt taactattta ttaaatgaat ttgttaata
//
LOCUS       DAXWFK010000143         6249 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.144, whole genome shotgun sequence.
ACCESSION   DAXWFK010000143 DAXWFK010000000
VERSION     DAXWFK010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6249
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.144"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(78..185)
                     /locus_tag="ACGEXS_002134"
     CDS             complement(78..185)
                     /locus_tag="ACGEXS_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013125.1"
                     /translation="MVLLAFVTTIHDTDDGRFFNEHKHANNKLLKHSLI"
     gene            complement(222..545)
                     /gene="isdG"
                     /locus_tag="ACGEXS_002135"
     CDS             complement(222..545)
                     /gene="isdG"
                     /locus_tag="ACGEXS_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylobilin-forming heme oxygenase IsdG"
                     /protein_id="HFF8013126.1"
                     /translation="MKFMAENRLTLTKGTAKDIIERFYTRHGIETLEGFDGMFVTQTL
                     EQEDFDEVKILTVWKSKQAFTDWLKSDVFKAAHKHIRSKNEDESSPIINNKVITYDIG
                     YSYMK"
     gene            complement(564..1298)
                     /gene="srtB"
                     /locus_tag="ACGEXS_002136"
     CDS             complement(564..1298)
                     /gene="srtB"
                     /locus_tag="ACGEXS_002136"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HFF8013127.1"
                     /translation="MRMKRFLTIVQILLVVIIIIFGYKIVQTYIEDKQERANYEKLQQ
                     KFQMLMSKHQEHVRPQFESLEKINKDIVGWIKLSGTSLNYPVLQGKTNHDYLNLDFER
                     EHRRKGSIFMDFRNELKNLNHNTILYGHHVGDNTMFDVLEDYLKQSFYEKHKIIEFDN
                     KYGKYQLQVFSAYKTTTKDNYIRTDFENDQDYQQFLDETKRKSVINSDVNVTVKDRIM
                     TLSTCEDAYSETTKRIVVVAKIIKVS"
     gene            complement(1360..2325)
                     /gene="isdF"
                     /locus_tag="ACGEXS_002137"
     CDS             complement(1360..2325)
                     /gene="isdF"
                     /locus_tag="ACGEXS_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin ABC transporter permease protein IsdF"
                     /protein_id="HFF8013128.1"
                     /translation="MIKNKKKLLFLCLLVILIATAYISFVTGTIKLSFNDLFTKFTTG
                     SNEAVDSIIDLRLPRILIALMVGAMLAVSGALLQAALQNPLAEANIIGVSSGALIMRA
                     LCMLFIPQLYFYLPLLSFIGGLIPFLIIILLHSKFRFNAVSMILVGVALFVLLNGVLE
                     ILTQNPLMKIPQGLTMKIWSDVYILAVSALLGLILTLLLSPKLNLLNLDDIQARSIGF
                     NIDRYRWLTGLLAVFLASATVAIVGQLAFLGIIVPHVVRKLVGGNYRVLIPFSTVIGA
                     WLLLVADLLGRVIQPPLEIPANAILMIVGGPMLIYLICQSQRNRI"
     gene            complement(2338..3216)
                     /gene="isdE"
                     /locus_tag="ACGEXS_002138"
     CDS             complement(2338..3216)
                     /gene="isdE"
                     /locus_tag="ACGEXS_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     IsdE"
                     /protein_id="HFF8013129.1"
                     /translation="MRIIKYLTILVISVVILTSCQSSSSQESTKSGEFRIVPTTVALT
                     MTLDKLDLPIVGKPTSYKTLPNRYKDVPEIGQPMEPNVEAVKKLKPTHVLSVSTIKDE
                     MQPFYKQLNMKGYFYDFDSLKGMQKSITQLGDQFNRKAQAKELNDHLNSVKQKIENKA
                     AKQKKHPKVLILMGVPGSYLVATDKSYIGDLVKIAGGENVIKVKDRQYISSNTENLLN
                     INPDIILRLPHGMPEEVKKMFQKEFKQNDIWKHFKAVKNNHVYDLEEVPFGITANVDA
                     DKAMTQLYDLFYKDKK"
     gene            complement(3213..4289)
                     /gene="isdD"
                     /locus_tag="ACGEXS_002139"
     CDS             complement(3213..4289)
                     /gene="isdD"
                     /locus_tag="ACGEXS_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated surface determinant protein IsdD"
                     /protein_id="HFF8013130.1"
                     /translation="MRNVKQIATKSIIAIISLGILTCTTMIGSVLADEIKYPSAKFNQ
                     PEAKDKTELTTSIFDEKIKENKALELLIFNQENKNVTEEQQLVDEKAQLISDMTGKIY
                     LQVKLKGQIDKEQLVFQNDKNEEFPFVIKDEKDDTIVRILIEQHMDKINMHVKTLAEK
                     KNLDNKEMVYSIHFKEKKVQHDDAKEVPSKHQNQENNQDQLKKDIDDKKDSQKSDTKE
                     RRTSLFTEKGLNDIPVQKDKVQQDSNKKIENERPKASGTLKVENSPPTIKKVENNHKE
                     QPKHKDEKSKKEKKKVVEKEKALPAFNRDDDSKNSSQLSSDIKELDEPNHKKQYMLFA
                     AGIVLATILLISAHLYSRKRGNQV"
     gene            complement(4289..4972)
                     /gene="isdC"
                     /locus_tag="ACGEXS_002140"
     CDS             complement(4289..4972)
                     /gene="isdC"
                     /locus_tag="ACGEXS_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0030492 - hemoglobin binding [Evidence
                     IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme uptake protein IsdC"
                     /protein_id="HFF8013131.1"
                     /translation="MKNILKVFNTTILALIIIIATFSNSANAADSGTLNYEVYKYNTN
                     DTSIANDYFNKPAKYIKKNGKLYVQITVNHSHWITGMSIEGHKENIISKNTAKDERTS
                     EFEVSKLNGKIDGKIDVYIDEKVNGKPFKYDHHYNITYKFNGPTDVAGANAPGKDDKN
                     SASGSDKGSDGTTTGQSESNSSNKDKVENPQTNAGTPAYIYAIPVASLALLIAITLFV
                     RKKSKGNVE"
     gene            5181..6233
                     /gene="isdA"
                     /locus_tag="ACGEXS_002141"
     CDS             5181..6233
                     /gene="isdA"
                     /locus_tag="ACGEXS_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored heme-scavenging protein IsdA"
                     /protein_id="HFF8013132.1"
                     /translation="MTKHYLNSKYQSEQRSSAMKKITMGTASIILGSLVYIGADSQQV
                     NAATEATNATNNQSTQVSQATSQPINFQVQKDGSSEKSHMDDYMQHPGKVIKQNNKYY
                     FQTVLNNASFWKEYKFYNANNQELATTVVNDNKKADTRTINVAVEPGYKSLTTKVHIV
                     VPQINYNHRYTTHLEFEKAIPTLADAAKPNNVKPVQPKPAQPKTPTEQTKPVQPKVEK
                     VKPTVTTTSKVEDNHSTKVVSTDTTKDQTKTQTAHTVKTAQTAQEQNKVQTPVKDVAT
                     AKSESNNQAVSDNKSQQTNKVTKHNETPKQASKAKELPKTGLTSVDNFISTVAFATLA
                     LLGSLSLLLFKRKESK"
BASE COUNT         2037 a         1110 c          800 g         2302 t
ORIGIN      
        1 caaaatatca ttattaattc ttttcttctt caaaataaat caaaatgata taattgatga
       61 ttattttcaa agcacattca aatcaaacta tgttttagca atttgttgtt agcatgtttg
      121 tgttcattaa aaaaacgacc atcatcggta tcatgtatgg tcgttacaaa agctaacaat
      181 accaattgtc ataacaagta ctgcaacctc tttaaattca attatttcat gtaactatag
      241 cctatatcat atgtaattac tttgttattt ataatcgggc tactttcatc ttcattttta
      301 cttctaatat gtttatgcgc tgctttaaag acatcagatt ttaaccaatc cgtaaaagct
      361 tgctttgatt tccaaactgt taaaattttc acttcatcaa aatcttcttg ttctaaagtt
      421 tgtgtaacaa acatgccatc aaagccttct aatgtttcaa tcccatgtct cgtgtaaaat
      481 cgttctataa tatcttttgc tgttcctttt gttaacgtca gcctattttc tgccataaat
      541 ttcataatta tcctcttttc tgtttaactt accttaatta tttttgcgac aacaacaatt
      601 cttttcgtcg tttcactata tgcatcttcg cacgttgata aagtcattat tctatctttt
      661 accgttacat taacatctga attaattaca gatttacgtt ttgtctcatc taaaaattgt
      721 tgataatctt gatcattttc aaaatctgta cgtatgtaat tatctttagt agtagtttta
      781 tatgcactaa atacttgcaa ttgatattta ccatatttat tgtcaaattc aattatcttg
      841 tgtttttcat aaaacgattg ctttaaataa tcttctaaca catcaaacat cgtattatca
      901 ccgacatggt gcccgtataa aatagtatta tgatttaaat tcttcaattc atttctaaaa
      961 tccataaaaa tactaccttt acgtcgatgt tctcgctcaa aatctaaatt taaataatcg
     1021 tgatttgtct taccttgtag tactggataa tttaatgatg ttcctgataa ttttatccat
     1081 ccaacaatgt ctttatttat tttttcaagt gattcaaatt gtggtctcac atgttcttga
     1141 tgtttgctca tcagcatttg aaatttttgt tgtaatttct cataatttgc gcgttcttgc
     1201 ttgtcttcaa tatatgtttg aacaattttg taaccaaaaa tgataataat tacaaccaat
     1261 aaaatttgta caatagttaa aaatcgcttc attctcataa aaatcctctt ttattaacga
     1321 cgtttcttca gtcatcacta aaccagttgt tgtaccgttt tagattcgat ttcgttgact
     1381 ttgacaaatt aagtaaatta gcattggacc accgacaatc attaaaatag cattggctgg
     1441 aatttctaaa ggaggctgta tcactcgtcc taataaatca gccactaaca atagccatgc
     1501 accaataact gtagaaaacg gaataagtac tctgtaattg cccccaacta gctttctaac
     1561 cacatgtggc acaataatac ctaaaaaggc tagttgtcca acaatcgcaa cagttgcact
     1621 tgctaaaaat actgctaata aacctgttaa ccatctgtaa cgatcaatat taaaaccgat
     1681 acttcgcgct tgtatgtcgt ctaaatttag taaattcaat ttaggggaca atagtaatgt
     1741 taatattaat cccaataatg ctgatactgc taatatgtat acgtcgctcc atattttcat
     1801 tgttaagcct tgaggaattt tcattaaagg gttttgagtt aaaatttcta aaacaccatt
     1861 taataatacg aataacgcaa cacctactaa tatcatactt acagcattga atctaaattt
     1921 agaatgcaac aatataatta ttaaaaatgg tattaaacct ccaataaaac ttaataatgg
     1981 taagtaaaag tacaattgtg gaataaacaa catacaaagt gctctcatta taagtgcacc
     2041 tgaggaaacg ccaatgatat tcgcctctgc caaaggattt tgtagtgctg cttgtaataa
     2101 tgctccagaa actgctaaca ttgcgccaac catcaatgca attaatatac gtggcaatcg
     2161 caaatcaatg attgaatcca ctgcttcatt gctaccagtt gtaaattttg taaataggtc
     2221 attaaatgac aatttaattg taccggttac aaacgaaata taagcagttg cgattaaaat
     2281 gactaacaaa cataaaaata gtagtttctt tttatttttt atcatcatat caactcacta
     2341 ttttttatcc ttataaaata aatcatataa ttgagtcatt gccttatcag catcaacatt
     2401 tgctgtaata ccgaatggca cttcctctaa gtcataaaca tgattatttt tcacagcttt
     2461 aaaatgtttc caaatatcat tttgtttaaa ttctttttga aacattttct taacttcttc
     2521 aggcattccg tgtggtaatc gtaaaataat atctggattg atattcaaca aattttcagt
     2581 attagacgaa atatattgac gatctttcac tttaataaca ttttctccac ctgctatttt
     2641 aactaaatca ccaatatatg atttatcagt tgctactaaa tagctacccg gtacacccat
     2701 taatattaat actttgggat gtttcttttg tttagctgct ttattttcaa ttttttgctt
     2761 tacagaattt aaatggtcat ttaattcttt tgcttgtgct ttacgattaa attgatcacc
     2821 taattgtgta atcgactttt gcatcccttt taaactatca aaatcataaa agtagccttt
     2881 catatttaat tgtttgtaaa atggttgcat ttcatcttta atcgttgaca cactcaaaac
     2941 atgtgttggt tttaactttt taacagcttc aacattcggc tccattggtt gaccaatttc
     3001 cggtacatct ttataacgat taggcaatgt cttatatgac gtgggtttgc cgacaattgg
     3061 taaatccaat ttgtccaatg tcattgtcaa tgcaacagtt gttggtacga ttctgaattc
     3121 gccggattta gttgattctt gagaactgga agattgacag ctggttaaga taacgacgct
     3181 tatcactaaa atggttaaat actttatgat tctcaaactt ggttacctct ctttctgctg
     3241 tataaatgtg ccgaaataag taaaatagtt gctaacacaa tgccagctgc aaataacata
     3301 tattgctttt tatgatttgg ttcatcaagt tctttaatat cactagataa ttgactacta
     3361 ttcttgctat catcatctct attaaaagct ggtaacgctt tttctttttc aactactttt
     3421 ttcttttcct tttttgattt ttcatcttta tgtttcggtt gctctttgtg attattttca
     3481 acctttttta ttgttggagg gctattttca acttttaatg tacctgatgc tttaggtcgc
     3541 tcattttcaa tctttttatt actgtcttgt tgcactttat ctttttgtac aggaatatca
     3601 tttaatcctt tttcagtaaa aaggctagta cgtctttcct tagtatctga tttttgacta
     3661 tcttttttgt catcaatatc ttttttaagc tgatcttgat tattttcttg attttgatgt
     3721 tttgaaggca cttcttttgc atcatcatgt tgtacttttt tctctttaaa atgaatagaa
     3781 tacaccattt ctttgttatc tagatttttc ttttcagcca acgttttaac atgcatattg
     3841 attttatcca tatgctgttc aattaaaatt cttactattg tgtcatcctt ttcatctttt
     3901 ataacaaaag gaaattcttc atttttgtca ttttgaaaaa caagttgttc tttatctatt
     3961 tgacctttta gctttacttg caagtaaatt ttaccagtca tatctgaaat caattgcgcc
     4021 ttttcgtcaa ctagttgttg ttcctcagtt acatttttat tttcttgatt aaaaattagt
     4081 aactctaacg ctttattctc ttttatcttt tcatcaaaaa ttgatgtagt taattctgtt
     4141 ttatcttttg cttcaggttg attaaatttg gctgatggat attttatctc atcagccaac
     4201 acgctaccaa tcattgttgt acatgtaagt atacctaagc taataatagc tataatagat
     4261 tttgtagcaa tttgtttaac atttctcatt attccacatt gcctttagat ttttttctaa
     4321 caaacaatgt gattgcgatt aataatgcta aggatgcaac tggtattgca tatatatatg
     4381 caggtgtacc agcatttgtt tgtggatttt ctactttgtc tttattcgaa ctattagatt
     4441 cactttgacc agtagtcgtt ccatcagatc ctttgtcact acctgaagca gaatttttat
     4501 catctttacc tggtgcatta gcacctgcta catcagttgg tccattaaat ttatatgtaa
     4561 tgttgtaatg atggtcatat ttgaatggct ttccatttac tttttcatcg atataaacgt
     4621 caatttttcc atctatttta ccgttcaact tacttacttc aaattcagaa gtgcgttcat
     4681 ctttggcagt gtttttacta ataatatttt ctttatgtcc ttcgatactc attccagtaa
     4741 tccaatgact gtggttgaca gttatttgaa catacaattt accatttttc ttaatgtact
     4801 ttgccggttt attaaaatag tcattagcaa ttgacgtgtc attggtattg tatttgtaaa
     4861 cctcataatt caaagtaccg ctatctgcgg catttgcaga attactgaat gtcgcgatga
     4921 tgataattaa cgctaaaatc gttgtattaa aaacttttaa aatatttttc aaaacataat
     4981 cctccttttt atgattgctt ttaagtcttt agtaaaatca taaataataa tgattatcat
     5041 tgtcaatatt tattttataa tcaatttatt attgttatac gaaaatagat gtgctagtat
     5101 aattgataac cattatcaat tgcaatggtt aatcatctca tataacaaca cataatttgt
     5161 atccttagga ggaaaacaac atgacaaaac attatttaaa cagtaagtat caatcagaac
     5221 aacgttcatc agctatgaaa aagattacaa tgggtacagc atctatcatt ttaggttccc
     5281 ttgtatacat aggcgcagac agccaacaag tcaatgcggc aacagaagct acgaacgcaa
     5341 ctaataatca aagcacacaa gtttctcaag caacatcaca accaattaat ttccaagtgc
     5401 aaaaagatgg ctcttcagag aagtcacaca tggatgacta tatgcaacac cctggtaaag
     5461 taattaaaca aaataataaa tattatttcc aaaccgtgtt aaacaatgca tcattctgga
     5521 aagaatacaa attttacaat gcaaacaatc aagaattagc aacaactgtt gttaacgata
     5581 ataaaaaagc ggatactaga acaatcaatg ttgcagttga acctggatat aagagcttaa
     5641 ctactaaagt acatattgtc gtgccacaaa ttaattacaa tcatagatat actacgcatt
     5701 tggaatttga aaaagcaatt cctacattag ctgacgcagc aaaaccaaac aatgttaaac
     5761 cggttcaacc aaaaccagct caacctaaaa cacctactga gcaaactaaa ccagttcaac
     5821 ctaaagttga aaaagttaaa cctactgtaa ctacaacaag caaagttgaa gacaatcact
     5881 ctactaaagt tgtaagtact gacacaacaa aagatcaaac taaaacacaa actgctcata
     5941 cagttaaaac agcacaaact gctcaagaac aaaataaagt tcaaacacct gttaaagatg
     6001 ttgcaacagc gaaatctgaa agcaacaatc aagctgtaag tgataataaa tcacaacaaa
     6061 ctaacaaagt tacaaaacat aacgaaacgc ctaaacaagc atctaaagct aaagaattac
     6121 caaaaactgg tttaacttca gttgataact ttattagcac agttgccttc gcaacacttg
     6181 cccttttagg ttcattatct ttattacttt tcaaaagaaa agaatctaaa taaatcatcg
     6241 tcacactca
//
LOCUS       DAXWFK010000144         6222 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.145, whole genome shotgun sequence.
ACCESSION   DAXWFK010000144 DAXWFK010000000
VERSION     DAXWFK010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6222
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.145"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..825
                     /gene="graS"
                     /locus_tag="ACGEXS_002142"
                     /gene_synonym="apsS"
     CDS             <1..825
                     /gene="graS"
                     /locus_tag="ACGEXS_002142"
                     /gene_synonym="apsS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase GraS/ApsS"
                     /protein_id="HFF8013133.1"
                     /translation="DKDKEIEEIKHKDLAETPFQRHTVDYLYRQISAHKEKVVEQQLQ
                     LNMHEQTITEFVHDIKTPVTAMKLLIDQEKNQERKQALLYEWSRINSMLDTQLYITRL
                     ESQRKDMYFDYVSLKRMVIDEIQLTRHISQVKGIGFDVDFKVDDYVYTDIKWCRMIIR
                     QILSNALKYSENFNIEIGTELNDQHVSLYIKDYGRGISKKDMPRIFERGFTSTANRNE
                     TTSSGMGLYLVNSVKDQLGIHLQVTSTVGKGTTVRLIFPLQNEIVERMSEVTNLSF"
     gene            969..1730
                     /gene="vraF"
                     /locus_tag="ACGEXS_002143"
     CDS             969..1730
                     /gene="vraF"
                     /locus_tag="ACGEXS_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein VraF"
                     /protein_id="HFF8013134.1"
                     /translation="MAILEVKQLTKIYGTKKMAQEVLRDINMSIEEGEFIAIMGPSGS
                     GKTTLLNVLSSIDYISQGSITLKGKKLEKLSNKELSDIRKHDIGFIFQEYNLLHTLTV
                     KENIMLPLTVQKLDKEHMLNRYEKVAEALNILDISDKYPSELSGGQRQRTSAARAFIT
                     LPSIIFADEPTGALDSKSTQDLLKRLTRMNEAFKSTIIMVTHDPVAASYANRVVMLKD
                     GQIFTELYQGDDDKHTFFKEIIRVQSVLGGVNYDL"
     gene            1720..3609
                     /gene="vraG"
                     /locus_tag="ACGEXS_002144"
     CDS             1720..3609
                     /gene="vraG"
                     /locus_tag="ACGEXS_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease VraG"
                     /protein_id="HFF8013135.1"
                     /translation="MTFNEIIFKNFRQNLSHYAIYLFSLITSVVLYFSFVALKYAHKL
                     NMTESYPIIKEGSQVGSYFLFFIIIAFLLYANVLFIKRRSYELALYQTLGLSKFNIIY
                     ILMLEQLLIFIITAILGIIIGIFGSKLLLMIVFTLLGIKEKVPIIFSLRAVFETLMLI
                     GVAYFLTSAQNFILVFKQSISQMSKNNQVKETNHNKITFEEVVLGILGIVLITTGYYL
                     SLNIVQYYDSIGTLMFILLSTVIGAYLFFKSSVSLVFKMVKKFRKGVISVNDVMFSSS
                     IMYRIKKNAFSLTVMAIISAITVSVLCFAAISRASLSSEIKYTAPHDVTIKDQQKANQ
                     LASELNNQKIPHFYNYKEVIHTKLYKDNLFDVKAKEPYNVTITSDKYIPNTDLKRGQA
                     DLFVAEGSIKDLVKHKKHGKAIIGTKKHHVNIKLRKDINKIYFMTDVDLGGPTFVLND
                     KDYQEIRKYTKAKHIVSQFGFDLKHKKDALALEKAKNKVDKSIETRSEAISSISSLTG
                     ILLFVTSFLGITFLIAVCCIIYIKQIDETEDELENYSILRKLGFTQKDMARGLKFKIM
                     FNFGLPLVIALSHAYFTSLAYMKLMGTTNQIPVFIVMGLYICMYAVFAVTAYNHSKRT
                     IRHSI"
     gene            4334..4951
                     /locus_tag="ACGEXS_002145"
     CDS             4334..4951
                     /locus_tag="ACGEXS_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HFF8013136.1"
                     /translation="MFSKKKDKFMVQLEEMVFNLDRAAIEFGKMDFNTHLDLKAYSDN
                     IKTYESHGDELVHQVITDLNQTFITPIEREDILSLCDAIDDVLDAIEETAAMFEMYSI
                     EYTDEYMAEFVDNIQKAVAEMKLAVGLLVDKKLSHMRIHSINIKEFETNCDGILRQSI
                     KHIFNSETDPITLIKIKDIYESMEEIADKCQIVANNFETIIMKNS"
     gene            4967..5974
                     /locus_tag="ACGEXS_002146"
     CDS             4967..5974
                     /locus_tag="ACGEXS_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFF8013137.1"
                     /translation="MSYIIIVTIAVVIFSLIFDFINGFHDTANAVATAVSTRALTPKT
                     AILMAAVMNFIGALTFTGVAGTITKDIVDPFKLENGLVVVLAAILAAIIWNLATWFYG
                     IPSSSSHALIGSIAGAAIASEGSFGVLHYQGFTKIIIVLIVSPIIAFCVGFLMYSIFK
                     VIFKNANLTRANRNFRFFQIFTAALQSFSHGTNDAQKSMGIITLALIVANVQNDGSVE
                     PQLWVKFACATAMGLGTAIGGWKIIKTVGGNIMKIRPANGAAADLSSALTIFVASSLH
                     FPLSTTHVVSSSILGVGASNRAKGVKWSTAQRMIITWVITLPISALLAGLLFYILNLF
                     F"
BASE COUNT         2252 a          804 c         1079 g         2087 t
ORIGIN      
        1 gacaaagata aagaaataga agaaattaaa cataaagatt tagcggaaac gccatttcaa
       61 cgtcatacag ttgattattt atatcgtcaa atctcagcgc acaaagaaaa ggttgttgag
      121 caacagttgc aattgaacat gcatgaacaa accattacag aatttgtgca cgacataaaa
      181 acacctgtga cagctatgaa attattaatt gatcaagaaa aaaatcaaga aagaaaacaa
      241 gcattactat atgaatggtc tcgtataaac tcgatgctag atacacagct gtatattact
      301 agattagaat ctcaacgtaa agatatgtat tttgattacg tgtcacttaa acgcatggtc
      361 attgatgaaa tacaattaac aagacatatt agtcaggtta aaggtattgg ttttgatgtt
      421 gactttaaag tggatgatta tgtttataca gatataaaat ggtgtcgtat gattattaga
      481 caaattttgt caaacgcatt gaaatatagt gagaatttta atattgaaat tgggacagaa
      541 ttaaatgatc aacatgtttc gttatatatt aaagactatg gcagaggtat tagtaaaaaa
      601 gatatgccgc gaatatttga acgaggattt acgtcaacgg ctaacagaaa tgaaacgacg
      661 tcttcaggta tgggtctata tttagtaaat agtgtaaagg atcaattagg tattcacctg
      721 caagtcacgt cgactgttgg taaggggaca actgtcagat tgattttccc attacaaaat
      781 gaaattgttg aacgcatgtc ggaagtgaca aatttgtcat tttaaacatg cgttttgtta
      841 cttagaattg atacatcaat gcagcttcaa cgttataata agatagatgt tagtcatatg
      901 ttaaattgaa gatacaagtg ccaaagccta aaggaaatga agttaagata aattatagga
      961 gtgttaaagt ggcaatttta gaagtaaaac aattaacaaa aatatatgga actaaaaaaa
     1021 tggcacaaga agtgttgcga gatatcaata tgtctattga agaaggcgag tttattgcta
     1081 ttatgggtcc ctctggatct gggaaaacga cattattaaa tgttttaagt tcaattgatt
     1141 atatttcaca aggttctatt acattaaaag gaaaaaaatt agaaaagctt tcaaacaagg
     1201 aattatctga tatacgcaag catgatattg gttttatttt tcaagagtat aatttactgc
     1261 atacattgac tgttaaagaa aacataatgt taccactaac ggtacagaag ttagataaag
     1321 aacatatgtt aaatcgttat gaaaaagtag cagaagcatt aaatatattg gatattagtg
     1381 ataaatatcc ctctgaattg tctggtggac aaaggcaacg aacatcagct gccagagcat
     1441 ttataacatt gccttctatt atatttgctg acgaaccaac aggtgcactg gattctaaaa
     1501 gtactcaaga tttattaaaa cgattaacaa gaatgaatga agcatttaag tctacaatta
     1561 ttatggtaac gcatgatcct gttgcagcaa gctatgcaaa tcgagtagtg atgctaaaag
     1621 atggtcaaat tttcactgaa ttataccaag gggatgacga taaacatacc tttttcaaag
     1681 aaataatacg tgtacaaagt gttttaggtg gcgttaatta tgacctttaa cgagataata
     1741 tttaaaaatt tccgtcaaaa tttatcacat tatgccatct atcttttttc gttaattacg
     1801 agtgtagtat tgtattttag ctttgtagca ttaaaatacg ctcataaact aaacatgaca
     1861 gagtcatatc caattataaa ggaaggctca caagtcggaa gctactttct atttttcatc
     1921 ataattgcat ttttgttata tgccaatgtg ttatttatta aacgacgaag ttatgagctt
     1981 gcattatatc aaacattagg tttatctaaa ttcaacatta tttatatact aatgctcgaa
     2041 caattactaa tatttataat tacggcaata ttaggtatta ttattggtat ttttggttcg
     2101 aaactgttat taatgattgt ctttacatta ttaggaatta aagaaaaggt tccaattatt
     2161 tttagtttga gggcggtatt tgaaacatta atgttaatcg gtgtcgctta ttttttaaca
     2221 tctgctcaaa attttatatt agtgttcaaa caatctattt cacagatgtc aaagaataac
     2281 caggttaaag aaacaaatca taataaaatt acatttgaag aggttgtttt aggcatctta
     2341 ggtatagtat tgattaccac aggatactat ctatctttga acattgttca atattatgat
     2401 tctatcggta cacttatgtt tattttattg tcaactgtga ttggggcata cttatttttt
     2461 aaaagctctg tttctctagt ttttaaaatg gtgaagaagt ttagaaaagg tgttataagt
     2521 gtaaatgatg tcatgttctc atcatctatt atgtatcgta ttaagaaaaa tgctttttca
     2581 cttacggtca tggcaatcat ttcagcgatt actgtttcag ttctttgctt tgctgctata
     2641 agtagagcgt ccttatcaag tgaaataaaa tatactgcac cacacgacgt tacaattaaa
     2701 gaccaacaaa aagctaatca attagcaagt gaattaaaca atcaaaaaat tcctcatttt
     2761 tataattata aagaagtaat tcatacgaaa ttgtataaag ataatttatt tgatgtaaaa
     2821 gcgaaagaac catacaatgt aacaattact agtgataaat acatccctaa tactgatttg
     2881 aaacgtgggc aagctgattt atttgtagcg gaaggttcta tcaaagattt agtgaaacat
     2941 aagaagcatg gtaaggcaat tataggaacg aaaaaacatc atgttaatat taagttacgt
     3001 aaagatatta ataaaatcta ttttatgaca gatgttgatt taggtggacc aacgtttgtc
     3061 ttaaatgaca aagactatca agaaataaga aagtatacaa aggcaaagca tatcgtctct
     3121 caatttggat tcgatttgaa acataaaaaa gatgctttag cattagaaaa agcgaaaaat
     3181 aaagttgata aatctattga aacaagaagt gaagcgataa gctcaatatc aagtttaacc
     3241 ggaatattat tatttgtaac atcattttta ggtattacat tcttgattgc tgtatgttgc
     3301 attatataca taaagcaaat agatgaaacc gaagatgagt tagagaatta tagtattttg
     3361 agaaagcttg gatttacaca aaaagatatg gcaaggggac taaagtttaa aattatgttt
     3421 aattttgggt tacctttagt tattgcacta tcacatgcat attttacatc attagcatat
     3481 atgaaattaa tgggtacaac gaatcaaata ccggttttca tagtaatggg attatacatt
     3541 tgtatgtatg ctgtttttgc agtgacggct tataatcatt ccaagcgaac aattagacat
     3601 tccatataaa atatacagat ggctttcagt agagtagtgg attcggattc acgaactata
     3661 ctggaagctt tttattataa atgaagagaa gttatatttt tagcatgtat agttgaatac
     3721 tgggttaaaa taccatatta ataatgaagt aaaggtatga gtgattatga aagtgttttg
     3781 aatgaaatat atttaattgg tgatgctttt aattgaaaag attaacagga ttcaactttg
     3841 taaattgtat taaatgtgag aaaataaaag tatattcatt gagagatata tgagtcaatg
     3901 atcgttttaa acaagataag tgtattttaa tatgtaaaag ttatgtaata aatattgtat
     3961 cgttgcaaat ttcccaacta tatccattta caatttttag agttgtatag ataaaaaatt
     4021 gcctatacaa taactttttc aaaacaaatg cctactacta tttaaatttt atgtaaaagt
     4081 taagtttcgg aattgttaaa aatttgtaaa ttgaattgtg ggattgtaaa ttttgtgcta
     4141 ttttcatata agggcaaata cagtaaattg tttactgtga ggcatttttg aaattaatat
     4201 cagtacacta aaattatact gacgattgat aataacaaat ttgtatcatt agtttgtaaa
     4261 ttcacttgtc ttaatttgaa acaaattata actgaatgtg attggtgaca atcgcttaaa
     4321 tggaggattt taaatgttta gtaagaaaaa agataagttt atggttcaat tagaagagat
     4381 ggttttcaat ctggatcgtg ctgctattga attcggtaaa atggatttca atacacattt
     4441 agatttaaaa gcatactcag acaacattaa aacttatgag tcacatggtg acgaattagt
     4501 acatcaagta attactgatt taaatcaaac atttatcaca ccaattgaac gtgaagatat
     4561 tttatcatta tgtgatgcaa ttgatgatgt tttagatgca attgaagaaa cggcagctat
     4621 gtttgaaatg tattcaatcg aatacacaga tgaatatatg gctgagtttg ttgataacat
     4681 tcaaaaagca gttgcagaaa tgaaacttgc tgtcggctta ttagtcgata aaaaattatc
     4741 acatatgcgt attcattcaa ttaatattaa agaatttgaa acaaactgtg atggtatttt
     4801 aagacagtca attaaacata ttttcaatag cgaaacagat ccaatcactt taattaaaat
     4861 aaaagatatt tatgaaagca tggaagaaat cgctgataaa tgtcaaatcg tagcaaataa
     4921 ttttgaaact attattatga aaaatagcta aggggagtat atatttatgt catatataat
     4981 catcgtcact atagctgtag ttattttctc gctgatattt gactttatca atggattcca
     5041 tgatacagcc aatgcagtag ctactgctgt atctactaga gcgttaacgc ctaaaacggc
     5101 aattttaatg gcagcagtga tgaactttat aggtgcttta acatttacgg gcgttgcagg
     5161 caccattact aaagacattg tcgatccatt taaattggaa aatggattag ttgttgtgtt
     5221 agctgcaata cttgcggcta ttatttggaa tttagctact tggttttacg gaattccaag
     5281 ttcgtcttca catgcactta taggttcaat tgcgggtgca gcaatcgcat ctgaaggctc
     5341 atttggagtg ttacattacc aaggtttcac aaaaattatt attgtattaa tcgtttcacc
     5401 gattatcgca ttttgtgttg gtttcttgat gtattcaatt tttaaagtta tctttaaaaa
     5461 tgcaaattta acaagagcga atcgtaactt tagatttttc caaattttca cagcagcgtt
     5521 acaatcattc tctcacggta cgaatgatgc gcaaaaatca atgggtatta ttacgttggc
     5581 attgattgtc gctaatgtac agaatgatgg cagtgttgaa ccacagttat gggtaaaatt
     5641 tgcctgtgcg acagcaatgg ggcttggtac tgcaattggt ggctggaaaa ttatcaaaac
     5701 tgtaggtggt aatattatga aaatacgtcc agcaaatggt gctgcggccg atttatcatc
     5761 tgcattaaca atttttgttg catcatcgct acatttccca ttatcaacaa ctcacgttgt
     5821 gtcatcatca atcttaggtg ttggtgcttc taaccgagct aaaggtgtaa aatggagcac
     5881 tgcgcaacga atgatcatta catgggtgat tacattacct atttcagcat tgttagcagg
     5941 tttactattc tatatactta acttattttt ctaattgaaa ataaaactaa actgaacttc
     6001 agtatcacaa acatatggtg gtattgaagt tcagtttttt atgtaagtag ataacatatt
     6061 ttatagaatt tacgtagaat gaccaagaca aagcatttta attaggaggc tatattataa
     6121 atgtattcca tggaacataa tcagttaggt cattaaaata tatgtaagag caaaataatg
     6181 atttaatagt ttatattctt caatgaagga ataattaaat ag
//
LOCUS       DAXWFK010000145         6121 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.146, whole genome shotgun sequence.
ACCESSION   DAXWFK010000145 DAXWFK010000000
VERSION     DAXWFK010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6121
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.146"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(8..406)
                     /gene="tagD"
                     /locus_tag="ACGEXS_002147"
     CDS             complement(8..406)
                     /gene="tagD"
                     /locus_tag="ACGEXS_002147"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047348 - glycerol-3-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HFF8013138.1"
                     /translation="MKRVITYGTYDLLHYGHIELLRRAREMGDYLIVALSTDEFNQIK
                     HKKSYYDYEQRKMMLESIRYVDLVIPEKGWGQKEDDVEKFDVDVFVMGHDWEGEFDFL
                     KDKCEVIYLKRTEGISTTKIKQELYGKDAK"
     gene            complement(469..1530)
                     /locus_tag="ACGEXS_002148"
     CDS             complement(469..1530)
                     /locus_tag="ACGEXS_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFF8013139.1"
                     /translation="MRFTIIIPTCNNEATIRQLLISIESKEHYRILCIDGGSTDQTIP
                     MIERLQRELKHISLIQLQNASIATCINKGLMDIKMTDPHDSDAFMVIKPTSIVLPGKL
                     DRLTAAFKNNDNIDMVIGQRAYNYHGEWKLKSADEFIKDNRIVTLTEQPDLLSMMSFD
                     GKLFSAKFAELQCDETLANTYNHAILVKAMQKATDIHLVSQMIVGDNDIDTHATSNDE
                     DFNRYITEIMKIRQRVMEMLLLPEQRLLYSDMVDRILFNNSLKYYMNEHPAVTHTTIQ
                     LVKDYIMSMQHSDYVSQNMFDIINTVEFIGENWDREIYELWRQTLIQVGINRPTYKKF
                     LIQLKGRKFAHRTKSMLKR"
     gene            complement(1527..2630)
                     /gene="tarB"
                     /locus_tag="ACGEXS_002149"
     CDS             complement(1527..2630)
                     /gene="tarB"
                     /locus_tag="ACGEXS_002149"
                     /EC_number="2.7.8.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid glycerol-phosphate primase TarB"
                     /protein_id="HFF8013140.1"
                     /translation="MNVLIKKFYHLVVRILSKMITPQVIDKPHIVFMMTFPEDIKPII
                     KALNNSSYQKTVLTTPKQAPYLSELSDDVDVIEMTNRTLVKQIKALKSAQMIIIDNYY
                     LLLGGYNKTSNQHIVQTWHASGALKNFGLTDHQVDVSDKAMVQQYRKVYQATDFYLVG
                     CEQMSQCFKQSLGATEEQMLYFGLPRINKYYTADRATVKAELKDKYGITNKLVLYVPT
                     YREDKADNRAIDKAYFEKCLPGYTLINKLHPSIEDSDIDDVSSIDTSTLMLMSDIIIS
                     DYSSLPIEASLLDIPTIFYVYDEGTYDQVRGLNQFYKAIPDSYKVYTEEDLIMTIQEK
                     EHLLSPLFKDWHKYNTDKSLHQLTEYIDKMVTK"
     gene            complement(2729..3541)
                     /gene="tagG"
                     /locus_tag="ACGEXS_002150"
     CDS             complement(2729..3541)
                     /gene="tagG"
                     /locus_tag="ACGEXS_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acids export ABC transporter permease
                     subunit TagG"
                     /protein_id="HFF8013141.1"
                     /translation="MSAIGTVFKEHVKNFYLIQRLAQFQVKIINHSNYLGVAWELINP
                     VMQIMVYWMVFGLGIRSNAPIHGVPFVYWLLVGISMWFFINQGILEGTKAITQKFNQV
                     SKMNFPLSIIPTYIVTSRFYGHLGLLLLVIIACMFTGIYPSIHIIQLLIYVPFCFFLT
                     ASVTLLTSTLGVLVRDTQMLMQAILRILFYFSPILWLPKNHGISGLIHEMMKYNPVYF
                     IAESYRAAILYHEWYFMDHWKLMLYNFGIVAIFFAIGAYLHMKYRDQFADFL"
     gene            3888..4682
                     /gene="tagH"
                     /locus_tag="ACGEXS_002151"
     CDS             3888..4682
                     /gene="tagH"
                     /locus_tag="ACGEXS_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acids export ABC transporter
                     ATP-binding subunit TagH"
                     /protein_id="HFF8013142.1"
                     /translation="MNVSVNIKNVTKEYRIYRTNKERMKDALIPKHKNKTFFALDDIS
                     LKAYEGDVIGLVGINGSGKSTLSNIIGGSLSPTVGKVDRNGEVSVIAISAGLSGQLTG
                     IENIEFKMLCMGFKRKEIKAMTPKIIEFSELGEFIYQPVKKYSSGMRAKLGFSINITV
                     NPDILVIDEALSVGDQTFAQKCLDKIYEFKEQNKTIFFVSHNLGQVRQFCTKIAWIEG
                     GKLKDYGELDDVLPKYEAFLNDFKKKSKAEQKEFRNKLDESRFVIK"
     gene            complement(4743..5507)
                     /gene="tarA"
                     /locus_tag="ACGEXS_002152"
     CDS             complement(4743..5507)
                     /gene="tarA"
                     /locus_tag="ACGEXS_002152"
                     /EC_number="2.4.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047244 -
                     N-acetylglucosaminyldiphosphoundecaprenol
                     N-acetyl-beta-D-mannosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminyldiphosphoundecaprenol
                     N-acetyl-beta-D-mannosaminyltransferase TarA"
                     /protein_id="HFF8013143.1"
                     /translation="MTVEERSNTAKVDILGVDFDNTTMLQMVENIKTFFANQSTNNLF
                     IVTANPEIVNYATTHQAYLELINQASYIVADGTGVVKASYRLKQPLAHRIPGIELMDE
                     CLKIAHVNHQKVFLLGATNEVVEAAQYALQQRYPNISFAHHHGYIDLEDETVVKRIKL
                     FKPDYIFVGMGFPKQEEWIMTHENQFESTVMMGVGGSLEVFAGAKKRAPYIFRKLNIE
                     WIYRALIDWKRIGRLKSIPIFMYKIAKAKRKIKKAK"
     gene            5743..>6121
                     /locus_tag="ACGEXS_002153"
     CDS             5743..>6121
                     /locus_tag="ACGEXS_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HFF8013144.1"
                     /translation="MNYLSNFFNTTIQLNIYIIVIVAACYIAIHQYRHKPVLNYLDVI
                     LNYIPVLTHEFGHVLFNKLAGGRAKDLVIVTSPRERQQTLQQGFAITQSRHLAGQWLT
                     TIGGYFMPPIMLLIGLASSHYQIP"
BASE COUNT         1988 a         1061 c          880 g         2192 t
ORIGIN      
        1 atataattta tttagcatct ttaccatata attcttgttt gattttagtc gtcgaaatgc
       61 cttctgtacg ttttaaataa atgacttcac atttatcctt taagaagtcg aattcacctt
      121 cccagtcatg tcccataaca aaaacatcta catcaaattt ttcgacatcg tcttcttttt
      181 gtccccagcc cttttctgga atgactaaat cgacatagcg tattgattca agcatcattt
      241 ttcgttgttc ataatcataa taagattttt tatgtttaat ttgattaaat tcatctgttg
      301 ataatgctac tattaaataa tcgcccatct ctcttgcacg acgaagcaat tcgatatgac
      361 catagtgaag taagtcatat gtgccatatg ttattacacg tttcattaag ttatcctcct
      421 catattgtca catcatagga ttgcagttta tggtcatcaa tgtacacgtt atcgttttaa
      481 cattgatttt gttcgatgtg caaactttct ccctttaagt tgtatcaaga attttttata
      541 agtcggccta ttaatgccca cttgaattaa tgtttgtcgc cacaattcgt atatttctct
      601 atcccaattc tcaccaataa attcaactgt atttataatg tcaaacatgt tttgcgatac
      661 ataatcagaa tgctgcatag acataatata gtcttttacg agttgaattg tcgtgtgcgt
      721 tactgctggg tgttcgttca tataatattt taatgaatta ttgaataaaa tacgatcaac
      781 catatcacta tataatagcc tttgttcagg taatagtaac atttccatga ctcgttgtct
      841 tattttcata atttctgtga tatatctatt aaaatcttca tcgttacttg tagcatgtgt
      901 atctatatcg ttatctccga caatcatctg tgaaactaaa tgtatatccg tagctttttg
      961 catcgccttg acaagtattg cgtgattgta tgtgttagct aaagtttcgt cacactgtaa
     1021 ttcagcaaat ttagcactga ataactttcc gtcaaaagac atcattgata acaaatctgg
     1081 ttgttccgtt aatgtaacga ttcgattgtc tttaataaac tcatcagcac ttttcaattt
     1141 ccattcacca tggtaattgt aagctcgctg ccctattacc atatcaatat tatcattatt
     1201 tttgaaagca gcagttaacc tatctaattt acctggcaat acgattgatg ttggttttat
     1261 gaccataaat gcgtcactat catgtggatc tgtcattttg atatccatca aacctttatt
     1321 aatacacgta gctatcgaag cattttgtaa ttgtattaat gaaatatgct tgagttctct
     1381 ttgtaaccgt tcaatcatag gaattgtttg atcagtagaa ccaccatcaa tacaaaggat
     1441 tctatagtgt tctttactct caatagatat taacaattgt cgaattgttg cctcattatt
     1501 acatgtaggt atgattatcg taaacctcat tttgtcacca tcttatctat atattctgtg
     1561 agctgatgta aacttttatc agtattatac ttatgccaat ctttaaataa cggacttaat
     1621 agatgttctt tttcttgtat cgtcattatt aaatcttctt cagtatacac tttgtagcta
     1681 tccggtattg ctttgtaaaa ttgattcagg cctctcacct gatcatatgt tccttcatca
     1741 tacacataaa atatagttgg aatatctaac aagctagctt ctattggcag cgaactatag
     1801 tcgctaataa ttatatctga cattagcatt aatgtagacg tgtcgattga agatacgtca
     1861 tcaatgtctg aatcttcaat tgatggatgt aatttattaa tcagtgtata tcctggtaaa
     1921 catttttcaa aataagcttt atcaatagcc ctattatctg ctttatcttc tctatatgtt
     1981 ggtacatata ataccaactt atttgtaatt ccatatttat cctttaactc tgccttaacc
     2041 gttgctctat cagctgtgta atatttatta attctcggaa gcccaaaata cagcatttgc
     2101 tcttctgttg cacctaaaga ctgtttaaaa cattgtgaca tttgttcaca acccactaag
     2161 taaaaatccg tcgcttgata aactttacgg tactgctgaa ccattgcctt gtcagacaca
     2221 tcgacttgat gatctgttaa gccaaagttt tttaatgcac cacttgcatg ccacgtttga
     2281 acaatgtgtt gattagaagt cttattatat ccacctagta ataggtaata attatcgata
     2341 ataatcatct gcgcgctttt caaagcctta atttgtttta ccaatgttcg attagtcatt
     2401 tctatcacat caacatcgtc gctaagttca gataaataag gcgcttgttt tggtgttgtt
     2461 aaaacagttt tctgatacga cgaattattt aatgctttga tgataggctt aatatcttct
     2521 ggaaaagtca tcataaatac gatatgcggt ttatcaatca cttgaggcgt aatcatttta
     2581 gaaagtattc gaactaccaa atgataaaat ttctttatta aaacgttcat aatacaccaa
     2641 cttaatatgt tatttactta aattataaac aaaaatgaac cccacttcca tttattaatg
     2701 gttagcgggg tttcgtcata taaatatatt acaagaagtc tgcaaattga tctctatatt
     2761 tcatgtgtaa gtacgcacca attgcaaaga aaatggcaac aataccgaaa ttgtataaca
     2821 ttaatttcca atgatccatg aaataccatt cgtgatataa aattgctgca cggtatgatt
     2881 cagcaataaa gtaaactgga ttatatttca tcatttcatg aattaaacca ctgataccat
     2941 ggttctttgg tagccacaaa attggtgaaa agtaaaataa tattcttaat attgcttgca
     3001 ttaacatttg tgtatctcta actaacacac cgagtgttga tgttaataac gtcaccgagg
     3061 cagttaagaa aaaacaaaac ggtacatata tcaataattg aatgatatgt attgatggat
     3121 aaataccagt aaacatacat gcaattatca caagtaaaag taagcctaaa tgtccataaa
     3181 atctacttgt cacaatatat gtcggtatta tcgataacgg gaagttcatt ttcgatactt
     3241 gattaaactt ttgtgtaatt gctttagtac cttctaaaat accttggttg atgaagaacc
     3301 acatactgat accaaccaat aaccaataaa caaaaggtac accatgaatt ggtgcattac
     3361 ttcttattcc taatccaaaa accatccagt aaaccataat ttgcataaca gggttaatta
     3421 attcccaagc cacacctaaa tagttactat gattgataat tttaacttga aactgagcca
     3481 gtctttgaat taaataaaag ttctttacat gttctttaaa aactgttcct attgctgaca
     3541 ttccattaaa ccacactttc aaatgtttaa ctatttctct acttaactaa atagtattat
     3601 aataattgtt gtaaatacta tcactataca tggatgctat caaaattatt gtctagttct
     3661 ttaaaatatt agtttattac aaatacatta tagtatacaa tcatgtaagt tgaaataagt
     3721 ttagttttta aatatcattg ttatcattga tgattaacat tttgtgtcaa aacacccact
     3781 ctgataataa caaaatcttc tatacacttt acaacaggtt ttaaaattta acaactgttg
     3841 agtagtatat tataatctag ataaatgtga ataaggaagg tctacaaatg aacgtttcgg
     3901 taaacattaa aaatgtaaca aaagaatatc gtatttatcg tacaaataaa gaacgtatga
     3961 aagatgcgct cattcccaaa cataaaaaca aaacattttt cgctttagat gacattagtt
     4021 taaaagcata tgaaggtgac gtcatagggc ttgttggcat caatggttcc ggcaaatcaa
     4081 cgttgagcaa tatcattggc ggttctttgt cgcctactgt tggcaaagtg gatcgtaatg
     4141 gtgaagtcag cgttatcgca attagtgctg gcttgagtgg acaacttaca gggattgaaa
     4201 atatcgaatt taaaatgtta tgtatgggct ttaagcgaaa agaaattaaa gcgatgacac
     4261 ctaagattat tgaatttagt gaacttggtg agtttattta tcaaccagtt aaaaagtatt
     4321 caagtggtat gcgtgcaaaa cttggttttt caattaatat cacagttaat ccagatatct
     4381 tagtcattga cgaagcttta tctgtaggtg accaaacttt tgcacaaaaa tgtttagata
     4441 aaatttacga gtttaaagag caaaacaaaa ccatcttttt cgttagtcat aacttaggac
     4501 aagtgagaca attttgtact aagattgctt ggattgaagg cggaaagtta aaagattacg
     4561 gtgaacttga tgatgtatta cctaaatatg aagctttcct taacgatttt aaaaagaaat
     4621 ccaaagccga acaaaaagaa tttagaaaca aactcgatga gtcccgcttc gttattaaat
     4681 aaaccgaaaa aaccgagaat ctccatttaa ggatttcctc ggttttattt ttgtcatcat
     4741 gattatttcg ccttttttat ttttcttttt gctttggcta ttttatacat aaatattgga
     4801 atactcttta atctaccaat acgtttccaa tctattaatg ctctatatat ccattcaatg
     4861 tttaattttc taaagatata aggcgctctc tttttagccc cagcaaatac ttcaagagaa
     4921 ccacctacgc ccatcatcac tgtagattca aattggtttt catgtgtcat aatccattct
     4981 tcttgtttag ggaatcccat acctacaaat atgtaatcag gtttaaacag tttaattcgt
     5041 ttcactactg tctcatcttc taaatcaata taaccgtgat gatgtgcaaa cgatatgttt
     5101 ggatatcttt gttgcaatgc atattgtgcc gcttctacaa cttcattagt tgcccctagc
     5161 aaaaatactt tttgatgatt tacatgagca attttcaaac attcatccat caactcaata
     5221 ccaggtatac gatgcgctag aggttgcttt aaacgatacg aagctttgac tactcctgtc
     5281 ccatcagcaa caatatagct cgcttgattt attaactcta aatacgcttg atgtgtcgtc
     5341 gcgtaattca ctatttcagg gttggctgtt actataaaaa gattattcgt tgattgattt
     5401 gcaaaaaagg ttttaatatt ttcaaccatt tgcaacattg ttgtattatc aaaatcgacc
     5461 cctaaaatgt caactttggc tgtattggat ctttcttcaa cagtcataac aggaatctcc
     5521 tatcttattt cattattatg ttagtgtaat caatattaac agtatttata ttcatcctat
     5581 ttatatcgaa atgattttgc tttattttaa cacatattca ccttttcaaa aattaaaatt
     5641 agtttaacta aggtaagtct aaagtctaac gctattgatg ccaatatcat ttataataag
     5701 tgataagcaa actttaaatt aatatcttat atgaggttaa caatgaatta tttatcaaac
     5761 ttttttaata cgacaattca acttaatata tacattatcg ttattgttgc agcttgttat
     5821 attgctattc atcaatatag acataagcct gttttgaact atttagatgt cattttaaac
     5881 tatatccctg ttttaacaca tgaatttggt catgtactgt ttaataaact cgctggtggc
     5941 cgtgcaaaag atttagtcat tgtaacaagt ccacgagagc gacaacagac attacaacaa
     6001 ggattcgcaa ttacacaatc tagacatcta gcaggccaat ggcttacaac gattggtggt
     6061 tattttatgc cacctattat gttattgata ggtttagcgt cgagccatta tcagatacct
     6121 a
//
LOCUS       DAXWFK010000146         6114 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.147, whole genome shotgun sequence.
ACCESSION   DAXWFK010000146 DAXWFK010000000
VERSION     DAXWFK010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6114
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.147"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..1648)
                     /locus_tag="ACGEXS_002154"
     CDS             complement(<1..1648)
                     /locus_tag="ACGEXS_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFF8013145.1"
                     /translation="MSVTEVIFSFLGGLGIFLYGLKIMGDGLQASAGDRLRDILNKFT
                     SNPVLGVIAGIVVTILIQSSSGTTVITIGLVTAGFMTLKQAIGVIMGANIGTTVTAFI
                     IGIDLGEYAMPILALGAFLIFFFKRSKINNIGRILFGFGSLFFGLEFMGDAVKPLASL
                     DGFKQLMLDMSTNPILAVIVGAGLTALVQSSSATIGILQEFYQQDLISLNAAIPVLLG
                     DNIGTTITAILASLAGSIAAKRAALVHVIFNLIGVIIFTIFLPVVIHLISLLQDLWHL
                     KPAMTIAVSHGIFNITNTLIQLPFVAGLAWIVTKLVPGKDIADDYKPQHLNKDLVYHA
                     PGVALQETQKELQNVGQIVLSMFEDIREITKDDKKLIKKLEQKHQAVETINDSIRNYL
                     VRISTKAITKADVERLAVMFDVNRSILKVAELTEEYVAQLKRQHDEDIRITEDAQRGM
                     DKLFNHVAESFDKAIDMLDVYDKTKKDEIVERSRESFNIEHKLRKGHIKRLNRGECTT
                     KGGLLYIDMIGVLERIGYHSRNVSEALVGLNDDVPTDEEIA"
     gene            complement(1974..3749)
                     /locus_tag="ACGEXS_002155"
     CDS             complement(1974..3749)
                     /locus_tag="ACGEXS_002155"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFF8013146.1"
                     /translation="MYYSYGNYEAFARPKKPENVENKSAYLIGSGLASLAAACFLIRD
                     GQMEGSKIHILEELPKAGGSLDGENMPLKGYVVRGGREMENHFECLWDLFRSIPSLEI
                     DNASVLDEFYWLNKEDPNYSRCRVIEKQGQRLVTDGDFTLTKTAIKEILDLCLTNEED
                     LDDVKITDVFSDDFFNSNFWIYWKTMFAFEPWHSAMEMRRYLMRFVHHISGLADFSAL
                     KFTKYNQYESLVLPMVEYLKSHGVQFEYDVKVEDIKIDVTTSQKIAREILIDRNGNAE
                     SIKLTINDLVFVTNGSITESSTYGDNDTPAPPTDELGGSWTLWKNLARQSPEFGNPDK
                     FCQNIPKKSWFVSATSTTNNKEIIDTIESICKRDPLAGKTVTGGIITINDSAWQMSFT
                     INRQQQFKDQPENEISTWIYALYSDVNGDYIKKPITECSGNEICQEWLYHLGVSTDKI
                     EDLAKHASNTIPVYMPYITSYFMTRAIGDRPLVVPHQSQNLAFIGNFAETERDTVFTT
                     EYSVRTAMEAVYQLLNIDRGIPEVINSPFDLRVLMDAIYELNDHQDLREITKDSKMQK
                     LALAGFLKKIKGTYIESLLKEHKLL"
     gene            complement(3923..4795)
                     /locus_tag="ACGEXS_002156"
     CDS             complement(3923..4795)
                     /locus_tag="ACGEXS_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFF8013147.1"
                     /translation="MNKLRDTTFLSYLFTIILWGSAFPMIKIALNDFSAESLSAFRLI
                     LATIILLPFVIIKKLPTPELRDIPVIFILGFCGFVIYHTALNFGETLISAGISGILVS
                     TTPIFSSALAYIFLKEHFSKWNWLSSLVAFIGISIISISKDDYTTINVLGVFIILLAS
                     FSESLYFTFQKKYIEKYGFIAFTLYTIMASSPFMLIFIPEIINDIHGATFTSIVSVLY
                     LAIFPTIIPYVLLAYIVKSVGVSDATMSLYLTPIVSLLLSYLLLDELPTTLAIIGGII
                     TLLGVSLSNFFQNT"
     gene            4908..>6114
                     /locus_tag="ACGEXS_002157"
     CDS             4908..>6114
                     /locus_tag="ACGEXS_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFF8013148.1"
                     /translation="MAKYKDIASDIRDKIITGDWFYGMKIPSHRQLAIQYNVNRVTII
                     KSIELLEAEGFIYTKVGSGTYVNDYLNEAHITNKWSEMMLWSSQQRSQYTVQLINKIE
                     TDDSYIHISKGELGISLMPHIQLKKAMSNTASHIEDLSFGYNNGYGYIKLRDIIVERM
                     SKQGINVGRENVMITSGALHAIQLLSIGFLGQDAIIISNTPSYIHSTNVFEQLNFRHI
                     DVPYNQINEIDTIIDRFINFKNKAIYIEPRFNNPTGRSLTNEQKKNIITYSERHNIPI
                     IEDDIFRDIFFSDPTPSIKTYDKLGKVIHISSFSKTIAPAIRIGWIVASEKIIEQLAD
                     VRMQIDYGSSILSQMVVYEMLKNKSYDKHLVKLRYVLKDKRDFMLNILNNLFKDIAHW
                     EVPSGGYFVW"
BASE COUNT         2061 a         1000 c         1020 g         2033 t
ORIGIN      
        1 ttgcaatttc ttcatctgta ggtacatcat cgttaaggcc aacaagtgct tcagaaacat
       61 ttcgtgaatg ataaccgata cgttcaagaa caccaatcat atcgatatat agtaatccgc
      121 cttttgttgt acattcacca cgattaaggc gtttaatatg acctttgcgt agtttatgtt
      181 caatattaaa tgattctcta ctacgttcta caatttcatc ttttttcgtt ttgtcataaa
      241 catctaacat gtcgatggct ttatcaaatg actcagcaac atggttgaat aatttatcca
      301 taccgcgttg tgcatcttct gtaatgcgaa tatcttcatc atgttggcgt tttaattgag
      361 cgacatactc ttctgttagc tctgctactt ttaaaataga gcgattgaca tcaaacataa
      421 ctgctaaacg ctcaacgtct gccttcgtaa tggcttttgt agaaattcta actaaataat
      481 ttcgaatgct atcattgatt gtttcaacag cttgatgctt ttgttcaagc tttttgatca
      541 attttttatc gtcttttgta atttcgcgaa tgtcttcaaa cattgataag acaatctgac
      601 ccacattttg taattctttt tgagtttctt gtaatgcaac accaggtgcg tgataaacaa
      661 gatctttgtt taagtgctga ggtttatagt catcagcaat atctttacct gggacaagct
      721 ttgtaacaat ccatgctaaa cctgctacaa atggtaattg aatcaaagta tttgttatgt
      781 tgaagatacc atgtgatact gcaatcgtca tcgctggttt taagtgccat aaatcttgta
      841 acaaactaat caaatgaatc acaactggca agaaaattgt gaagataatt accccgatta
      901 agttaaagat gacgtgtaca agcgccgcac gttttgcagc gattgagccg gctaaactag
      961 ctaagatagc tgtaatcgtg gtaccaatgt tatcgcctag taacacaggg attgctgcgt
     1021 ttaagctaat taaatcttgt tgataaaatt cttgtaaaat accaatcgtc gcacttgaac
     1081 tttgaactag tgctgttaac cctgcgccga caatgacagc gagtattgga tttgtagaca
     1141 tatcaagcat taattgctta aatccatcta atgatgctaa aggtttaacg gcatcaccca
     1201 taaattctag accgaagaat agtgaaccga aaccgaatag tatgcggcca atgttattga
     1261 ttttagagcg tttaaagaaa aagattaaga atgcacctaa tgctaaaatt ggcattgcat
     1321 attcgcctaa atctataccg ataataaatg cagttaccgt tgttccgata ttagcaccca
     1381 ttatcactcc aatggcttgt ttcaatgtca taaatccagc tgttaccagt ccgattgtga
     1441 taactgtcgt acctgaacta ctttgtatta aaatagttac aacgatacct gcaataacac
     1501 ctaatactgg atttgatgta aatttgttta aaatatctcg tagcctgtct cctgctgatg
     1561 cttgaagccc gtctcccatg atttttaagc cgtaaaggaa aatacctaaa ccacctaaaa
     1621 aggagaaaat gacttctgta accgacattt ccattatttc acctcaaata agctttatat
     1681 ttagattatc gcttataatt gtaaatttaa tgttaagatt aggtaaaatt atttaacaat
     1741 atatgttatt tgtatatgac ttgtaaaata tcgtcactta ttatgtaaat tttcagtgtg
     1801 aaatggcagg tttgcaatca cttgtttaac aaaatgatgc aatcaatcat gtaattatgt
     1861 ttcatcaaaa aaatcatgag agtggaacaa cgaaataatt tttatgaaaa catcatttct
     1921 gtcccagtct catgatttga aatcaccaaa taaaaatcta ttaatggttt tcgttataac
     1981 aatttgtgtt cttttaataa tgactcaatg tacgtacctt ttatcttttt aaggaatcct
     2041 gctaatgcga gtttttgcat tttcgaatct ttagtaatct cacgcaaatc ttggtggtca
     2101 ttcagttcgt atatggcatc cattaagacg cgaagatcaa atggactatt gatgacttct
     2161 ggaataccac gatctatatt tagtaattga taaacagctt ccatggcagt acgaaccgaa
     2221 tattctgttg taaatacagt gtctcgctct gtttctgcaa agttaccaat aaatgctaag
     2281 ttctgagatt gatgcgggac gactaaaggt ctgtcgccga tagcacgcgt catgaaataa
     2341 gatgtgatat atggcatata aacaggaatc gtattagatg catgttttgc taagtcttca
     2401 attttgtcag ttgatacacc taagtgatac agccattctt ggcatatttc attaccacta
     2461 cattctgtaa ttggcttttt aatataatcg ccgtttacat ctgaatataa ggcataaatc
     2521 catgtagata tttcattttc aggttggtct ttaaactgtt gctgacgatt gattgtaaaa
     2581 ctcatttgcc atgcagaatc attgattgta ataataccgc ctgtaactgt tttgcctgca
     2641 agtgggtcac gtttacaaat actttctatt gtatcgataa tctctttatt gtttgttgta
     2701 gaagttgctg aaacaaacca acttttttta ggaatatttt ggcaaaactt atcaggatta
     2761 ccaaattcag gactttgtcg cgctaaattt ttccatagtg tccaactacc acctaattcg
     2821 tcagttggtg gcgctggtgt atcattatca ccataagtag agctttctgt aatactaccg
     2881 tttgtcacaa agacaagatc gtttatagtc agtttaatag attctgcatt accattacgg
     2941 tcaattaata tttctcgggc aattttttga cttgtcgtaa catctatttt aatatcttcg
     3001 acttttacat cgtattcaaa ttgaacccca tgcgatttta aatattcaac cataggtaat
     3061 actaaagatt catattgatt atatttagtg aattttaaag ctgaaaagtc tgcgagacca
     3121 ctaatatgat gaacgaatcg cattagatag cgacgcattt ccattgcaga atgccacggt
     3181 tcaaatgcaa acatcgtttt ccagtaaatc caaaagtttg aattaaagaa gtcatcggaa
     3241 aatacatctg ttattttgac atcatctaaa tcttcttcat tcgttaagca taaatctaaa
     3301 atttctttaa tcgccgtttt agtcaaagtg aagtctccgt ctgtgactaa acgttgaccc
     3361 tgtttctcaa taacacgaca gcgagaatag ttagggtctt ctttgtttag ccaatagaac
     3421 tcatctaata cagacgcgtt atcgatttct aatgaaggga tagatctgaa taagtcccac
     3481 aaacattcaa agtggttctc catttcacga ccaccgcgga caacatagcc ttttaaaggc
     3541 atattttcac catcaagact accacctgct ttaggtaact cttctaaaat atgaatcttc
     3601 gaaccttcca tttgaccatc ccttattaaa aaacaagctg cagcaagtga agctagacca
     3661 gatccgatta agtaagcgga tttgttttct acattttcag gttttttagg gcgcgcaaat
     3721 gcttcataat ttccataact gtaatacata tccaccaagt cctttcgtat tagaatacac
     3781 tcagactata cccctttgtg aattgcaaat attagtattt atattggtaa atttgacgaa
     3841 attagatcat ttttatattt ttgattttta aaattttatc actcgaatgt ttttggattc
     3901 tgaggtgcgg gacttggaat aattatgtat tttgaaagaa gttacttaaa ctaacgccta
     3961 gtagagtgat aattccgcct ataatagcaa gggttgttgg tagctcgtct aataacagat
     4021 aagataataa taaagaaacg ataggtgtta aataaagaga cattgttgca tcagagacac
     4081 caactgactt cacaatataa gcaagcaaaa cgtatggaat tatagtaggg aatatagcta
     4141 aataaagtac cgatactatt gatgtaaaag tggcgccgtg tatatcgttg atgatttcag
     4201 gaataaaaat aagcataaat ggtgagcttg ccattattgt atatagtgtg aaagcgatga
     4261 agccgtattt ttctatgtat tttttctgga aagtaaaata caaactttca ctaaaagatg
     4321 caagtaaaat aataaaaaca cctaatacat taatagttgt gtaatcatct ttacttattg
     4381 aaataatgga tattcctata aatgcgacga gtgaacttaa ccaattccat tttgaaaaat
     4441 gctcttttaa aaatatataa gctaaagcac tagaaaaaat aggcgttgta gagactagaa
     4501 ttccagatat acctgcacta atcaaagttt caccaaaatt taaagctgtg tgatatatca
     4561 caaatccaca aaatcctaaa ataaaaataa cagggatatc tcttagttca ggggtaggca
     4621 atttctttat aattacgaac ggcaagagaa ttattgttgc taaaattaaa cgaaatgccg
     4681 acaatgattc tgcactaaaa tcatttaacg caatctttat cattggaaat gcagatcccc
     4741 acaatatgat agtaaataaa tatgatagaa aagtagtgtc tcgaagttta ttcattaata
     4801 tcatcactcc tttaattatg tgtttctata ttaaaaaata tgatttaaaa tgagtacaac
     4861 caattttgaa tggatttacc tatccaattt taaaagggag ggagaagatg gctaaatata
     4921 aagatattgc tagtgacata agagataaaa taatcacagg ggattggttt tatggaatga
     4981 aaataccttc acataggcag ttggcgatac agtacaacgt aaatagagta acgattatta
     5041 aaagtattga gttattagaa gctgaaggat ttatctatac taaagtaggt agtggaacat
     5101 atgttaatga ctatttgaat gaagcacata ttacaaataa gtggtctgaa atgatgttat
     5161 ggtcctctca acaaagaagt cagtatacgg tgcaattaat taataaaatt gagacagatg
     5221 attcgtatat acatataagt aaaggtgaat tgggtatatc gttaatgcca catattcaat
     5281 tgaaaaaagc catgtctaat acagccagtc atattgaaga cttatctttt ggttataata
     5341 atggctatgg ttatatcaag ttaagagata ttatcgttga acgaatgtca aagcaaggta
     5401 taaatgtagg tagagaaaat gtaatgatca cttcaggcgc tttacatgcc attcaacttt
     5461 tatctattgg gtttttaggt caagatgcca taataatttc gaatacacca tcatatattc
     5521 actctacaaa tgtttttgag caattgaatt ttagacatat tgatgttcct tataatcaaa
     5581 ttaatgaaat tgataccatc attgatagat ttattaattt taaaaataaa gcgatttata
     5641 tagaacctag gtttaataac ccgacaggtc gttctttaac gaatgagcaa aagaaaaata
     5701 taattactta tagcgaaaga cataatattc ctatcattga agatgatatc tttagagata
     5761 ttttctttag cgatccaact ccttctatca aaacttatga taaattggga aaagttatac
     5821 atataagcag tttttcaaaa acgattgcac cagcaataag aataggttgg attgttgctt
     5881 ctgaaaaaat aatagagcaa ttggcagatg taagaatgca aattgactat ggatccagta
     5941 ttttgtcaca aatggttgta tatgagatgt tgaaaaataa gtcttatgat aaacacttag
     6001 taaagttaag gtatgtttta aaagataaac gagactttat gttaaacatc ctcaataatt
     6061 tatttaagga tatagcacat tgggaggttc caagtggagg ttattttgta tggt
//
LOCUS       DAXWFK010000147         6076 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.148, whole genome shotgun sequence.
ACCESSION   DAXWFK010000147 DAXWFK010000000
VERSION     DAXWFK010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6076
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.148"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(105..329)
                     /locus_tag="ACGEXS_002158"
     CDS             complement(105..329)
                     /locus_tag="ACGEXS_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499185.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HFF8013149.1"
                     /translation="MTKTLELSFKSSLDKPVKLQLPQLNNVVTEQVARDSMNALVDLN
                     IIKSNSGTITKVHSAQIIDKTTTVLFEDKK"
     gene            complement(346..549)
                     /locus_tag="ACGEXS_002159"
     CDS             complement(346..549)
                     /locus_tag="ACGEXS_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HFF8013150.1"
                     /translation="MSKINHITIVLSFTKVDANGKQTEFKRRFANINPDASNDQIKTF
                     SKLIERLTGETYSNIELIKSLSI"
     gene            708..1268
                     /locus_tag="ACGEXS_002160"
     CDS             708..1268
                     /locus_tag="ACGEXS_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8013151.1"
                     /translation="MNKVEAIKFNDDIVKMYEALKIKSERDYLFFKLAIHSGLKVSEL
                     LTITVSQVKRLIEKCTLSEMCKAHFHSLIKIRLPETLSKELLQYIEDRSLSNEDVLFQ
                     SLRTNQVLSRQQAYRIIHQASIEAGIDNVGLTTLRKTFAYHAYQKGIPIPVIQKYLGH
                     QSAIETLNFIGLENECEHSIYISLQL"
     gene            1287..3689
                     /gene="mnhA2"
                     /locus_tag="ACGEXS_002161"
     CDS             1287..3689
                     /gene="mnhA2"
                     /locus_tag="ACGEXS_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit A"
                     /protein_id="HFF8013152.1"
                     /translation="MSLVYLLIAILVIMAMILLMSKRRALAKYAGYIALVAPVISSIY
                     FLIQIPSVAKLQYLSTSIPWIKTLDINLDLRLDGLSLMFSLIISLIGIAVFFYATQYL
                     SSRKDNLPRFYFYLTLFMFSMIGIVLSDNTILMYIFWELTSVSSFLLISYWYNNGDSQ
                     FGAIQSFMITVFGGLALLVGFIMLYIMTGTNNITEILGQADHIKNHGLFIPMIFMFLL
                     GAFTKSAQFPFHIWLPRAMAAPTPVSAYLHSATMVKAGIFLLLRFTPLLGLSNMYVYI
                     VTFVGLITMLFGSITALKQWDLKGILAYSTISQLGMIMAMVGIGGGYAQHQQDAIASI
                     YVFVLFGALFHLMNHAIFKCALFMGVGILDHEAGSRDIRILSGMRQLFPKMNLVMTIA
                     ALSMAGVPFLNGFLSKEMFLDALTQTGQLSQFSLISMIAIVFVGVIASVFTFTYALYM
                     VKEVFWTKYDSKVFTKKNIHEPWLFSLPSLILMVLVPVIFFVPNIFGKGIIVLALRAV
                     SGGNHQIDQLAPHVSQWHGFNIPLLLTIIIILLGSVLAIKVDWKKVFTGKIRQISVSK
                     SYEMVYRHFEKFATKRFKRVMQDRLNQYIIMTLGIFMIIIGYGYIRIGLPKVHQLHVS
                     EFGALEIILAIVTVTIGISLIFIRQRLTMVILNGVIGFVVTLFFIAMKAPDLALTQLV
                     VETITTILFIVSFSRLPNVPRSNANKKREIIKISVSLLMALIVVSLIFITQQTDGLSS
                     ISDFYLKADKLTGGKNIVNAILGDFRALDTLFEGLVLIITGLGIYTLLNYQDRRGQDE
                     RE"
     gene            3676..4101
                     /gene="mnhB2"
                     /locus_tag="ACGEXS_002162"
     CDS             3676..4101
                     /gene="mnhB2"
                     /locus_tag="ACGEXS_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit B"
                     /protein_id="HFF8013153.1"
                     /translation="MKENDVVLRTVTKLVVFILLTFGFYVFFAGHNNPGGGFIGGLIF
                     SSAFILMFLAFNVEEVLESLPIDFRILMIIGTLVSSITAIIPMFFGKPFLSQYETTWI
                     LPILGQIHVSTITLFELGILFSVVGVIVTVMLSLSGGRS"
     gene            4098..4442
                     /gene="mnhC2"
                     /locus_tag="ACGEXS_002163"
     CDS             4098..4442
                     /gene="mnhC2"
                     /locus_tag="ACGEXS_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit C"
                     /protein_id="HFF8013154.1"
                     /translation="MNLILLLVIGFLVFIGTYMILSINLIRIVIGISIYTHAGNLIIM
                     SMGTYGSSRSEPLITGGNQLFVDPLLQAIVLTAIVIGFGMTAFLLVLVYRTYKVTKED
                     EIEGLRGEDDAK"
     gene            4432..5928
                     /gene="mnhD2"
                     /locus_tag="ACGEXS_002164"
     CDS             4432..5928
                     /gene="mnhD2"
                     /locus_tag="ACGEXS_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit D"
                     /protein_id="HFF8013155.1"
                     /translation="MLSNLLILPMLLPFLCALILVFLKNNDRISKYLYLGTMTITTII
                     SLMLLIYVQRHRPITLDFGGWSAPFGIQFLGDSLSLIMVTTASFVITLIMAYGFGRGE
                     HKANRYHLPSFILFLSVGVIGSFLTSDLFNLYVMFEIMLLASFVLITLGQSVEQLRAA
                     IIYVVLNIIGSWLFLLGIGLLYKTVGTLNFSHIAMRLNDMGENRTVTMISLIFLVAFS
                     AKAALVLFMWLPKAYAVLNTELAALFAALMTKVGAYALIRFFTLLFDQHNDLIHPLLA
                     TMAAITMVIGAIGVIAYKDIKKIAAYQVIISIGFIILGLGTNTFAGINGAIFYLVNDI
                     VVKTLLFFIIGSLVYITGYRQYQYLNGLAKKEPLFGVAFIIMIFAIGGVPPFSGFPGK
                     VLIFQGALQNGNYIGLALMIITSLIAMYSLFRILFYMYFGDKDGEEVNFKKIPLYRKR
                     ILSILVVVVIAIGIAAPVVLNVTSDATELNTSDQLYQKLVNPHLKGED"
     gene            5929..>6076
                     /gene="mnhE2"
                     /locus_tag="ACGEXS_002165"
     CDS             5929..>6076
                     /gene="mnhE2"
                     /locus_tag="ACGEXS_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit E"
                     /protein_id="HFF8013156.1"
                     /translation="MNQIVLNIIIAFLWVLFQDEDHFKFSTFFSGYLIGLIVIYILHR
                     FFSDD"
BASE COUNT         1905 a          847 c         1077 g         2247 t
ORIGIN      
        1 gttgaaaaaa acaggacatt attacaacaa aatcacccta gacaatcatg atgaaagtct
       61 agggtgttag tatttatact aaattaattg cgctttcttt atatctattt cttatcttca
      121 aataaaacag ttgttgtttt atcaataatt tgagcagaat gtactttggt gatagtacca
      181 ctatttgact ttataatatt taaatctact aatgcattca tactatcacg tgcaacttgc
      241 tcggtcacta cgttgtttaa ttgtggtaat tgcagtttta caggcttgtc tagtgaagat
      301 ttaaaactta attcaagtgt tttagtcatg aatatgtcct cctgattaaa ttgataaaga
      361 tttgatgagt tcgatattgc tatatgtttc tccagtaagt cgctcaataa gtttgctgaa
      421 tgttttaatt tggtcgtttg atgcatcagg gttaatgtta gcgaatcgac gcttaaattc
      481 tgtttgtttg ccgttagcgt ctactttagt aaatgataat acaatagtga tgtggtttat
      541 tttactcata ttttaaaacc tcctttcaca ctatatatcg aaacaaaata ataaaatggc
      601 taattttatt ttctatgttt aaaatctata aaaaaggcaa tagatatatg taactaaaat
      661 ataatatagc tatgattaag acacttaaag caaggggagg ttgagtaatg aataaagtag
      721 aagcgattaa atttaatgat gatattgtta aaatgtatga agcgctcaag ataaaatctg
      781 aacgtgacta tttattcttt aagttagcta tacatagtgg attgaaagta tcagaattat
      841 taacaattac agtctctcaa gttaagagac taattgaaaa gtgtacgtta tcagaaatgt
      901 gtaaagcaca ttttcattcg ttgattaaaa ttaggttacc agaaacatta tcgaaagaac
      961 tacttcaata tatagaggac aggagtcttt cgaatgaaga cgttcttttt caatcactac
     1021 gaacaaatca agtattatct agacagcaag catatcgaat aattcaccaa gcatcaattg
     1081 aagctggtat agataatgta ggactaacga cattgcgtaa gacatttgca tatcatgctt
     1141 atcaaaaagg tatacctata ccagtcattc aaaagtattt agggcatcaa tctgctattg
     1201 aaacactaaa ttttatcggt ttagaaaatg agtgtgaaca tagtatttat atttcattac
     1261 aattatagaa acaaaggagg ctaataatga gtttggttta tttattaatt gctatacttg
     1321 tgattatggc gatgatactt ctaatgtcta aacgtagagc attggctaaa tatgccgggt
     1381 acatagcgtt ggttgcacct gtaatttcat ctatctattt tttgattcaa ataccatcag
     1441 tagctaaact gcaatatctt tctacctcta ttccatggat taagacatta gatattaatt
     1501 tagatttacg tttagatggt ttaagtttaa tgttttctct tattatttca cttattggaa
     1561 ttgcagtatt cttctatgca actcaatatt tatcctctcg aaaagacaat ttaccaaggt
     1621 tttattttta tttaacgtta tttatgttca gtatgattgg tattgtatta tcagacaata
     1681 cgatattgat gtacattttt tgggaattaa cgagtgtatc atcattttta ttgatttcat
     1741 attggtataa caatggtgac agtcaatttg gtgcgattca atcatttatg attacagtat
     1801 ttggtggatt ggcgttatta gttggtttta ttatgctgta tatcatgaca ggaacgaata
     1861 acatcacaga gatattagga caagcagatc atattaagaa tcatggattg tttatcccta
     1921 tgatttttat gtttttatta ggtgcattta caaaatcagc acaatttcca tttcatattt
     1981 ggctacctag agcaatggct gcacctacac ctgtaagtgc ttatttacat tcagccacga
     2041 tggtaaaagc tggtatcttt ttattacttc gatttacacc attattaggt cttagcaata
     2101 tgtacgtata tatcgttacg tttgttggtt taataacaat gttatttggt tcaattacag
     2161 ctttaaaaca atgggattta aaaggtatcc tagcgtactc tacaatcagt caacttggga
     2221 tgattatggc tatggtgggt ataggtggcg gatatgctca acaccaacaa gacgcaatag
     2281 catctattta tgtatttgta ttatttggtg cgctatttca tctaatgaat catgccatct
     2341 ttaaatgtgc gcttttcatg ggagtaggta ttttagatca tgaagcaggt tcaagggata
     2401 tacgaatttt aagtggaatg cgtcaactat ttcctaaaat gaatctagtc atgacgatag
     2461 cggctctatc tatggctgga gtaccatttt taaatggatt tttaagtaaa gaaatgtttt
     2521 tagatgcatt aacacaaact ggacaattat cccaatttag tttgatttca atgatagcta
     2581 tcgtgtttgt tggtgttatt gcgagtgttt ttacattcac atatgcacta tacatggtaa
     2641 aagaagtatt ttggacaaaa tatgattcta aggtttttac taaaaaaaat atccacgaac
     2701 catggttgtt tagtttacca tctcttatat taatggtgct agtacctgta atcttttttg
     2761 taccaaatat atttgggaag gggattatcg ttctagcatt aagagctgta tcaggtggta
     2821 atcatcaaat tgatcaattg gcaccacatg tttcgcaatg gcatggattt aacataccgc
     2881 ttcttttaac catcatcatt attttattgg gtagtgtact agcaatcaaa gtagattgga
     2941 aaaaagtgtt cacaggtaaa attagacaga tttcagtttc aaaaagctat gagatggtat
     3001 atcgacattt tgaaaagttt gctacgaagc gatttaaacg tgttatgcaa gatcgtttaa
     3061 accaatacat tattatgacc ttaggcatat ttatgattat cattggatat ggttatattc
     3121 gaattggact tcctaaagta catcagttac atgtttctga atttggggca ttagaaatta
     3181 tattagcaat cgtaactgtc acaattggta tttctttaat ttttatacgt caacgactga
     3241 caatggtcat tttaaatgga gtcatcggat ttgttgtgac cttattcttt atagcaatga
     3301 aagcccctga tctagcattg actcagctag tagttgaaac aataacgacg atactattta
     3361 ttgtcagttt ttcaagatta ccaaacgtgc caagatctaa cgctaacaaa aaaagagaaa
     3421 taattaaaat ttctgtatca ctcttgatgg cacttattgt tgtatcatta atttttatta
     3481 cacaacaaac agatggttta tcatcaatat cagactttta tttaaaagct gacaaactaa
     3541 caggtggtaa aaatattgta aatgcgatac ttggtgactt tagagcatta gatacattat
     3601 ttgaaggatt agtgttaatt attactgggc taggtattta cacattatta aattatcaag
     3661 atcggagggg acaagatgaa agagaatgat gtcgtgttaa gaacggtcac gaaacttgtt
     3721 gtatttattt tattgacttt cggattctat gtcttcttcg caggtcataa taatcctggt
     3781 ggtgggttta ttggtggttt aatatttagt tcagcgttta ttttaatgtt tctggctttt
     3841 aatgttgaag aggttttaga aagtttaccg attgatttta gaattttaat gattattgga
     3901 acattggtat catctattac tgcgataata cctatgtttt ttggaaaacc atttttgtct
     3961 caatatgaaa caacttggat acttccaatt ttaggacaaa ttcatgtaag tacaataaca
     4021 ctttttgaat taggtatttt attctcagtt gttggtgtta ttgtcacagt gatgttgtcg
     4081 cttagcggag gtcgatcatg aatttaatat tattactagt tataggattt ttagtgttta
     4141 taggaacata tatgatttta tcaatcaatt taattcgtat tgtaatcgga atttcaatat
     4201 atactcatgc tggtaatctc attattatga gtatgggaac gtatggttct agtagatcag
     4261 aaccactaat aactggtgga aaccaattgt ttgttgatcc cttgttacaa gctattgtac
     4321 taactgcaat agttataggg tttgggatga ctgcgttttt acttgtactt gtttatagaa
     4381 cttataaagt aacaaaagaa gatgaaattg aaggcctaag gggggaagat gatgctaagt
     4441 aacttattga ttttaccaat gttattacca ttcctttgtg ccttaatcct tgtattttta
     4501 aaaaataatg atcgtatttc taaatattta tacttaggta caatgactat caccacaatt
     4561 atttcattaa tgctattaat ttatgttcag cgtcaccgtc caattacgct agactttgga
     4621 ggatggtcag cgccctttgg tatacagttt ttaggagatt ctttaagttt aattatggtt
     4681 acaaccgctt cgtttgtgat tactttaatt atggcatacg gatttgggcg tggcgaacat
     4741 aaagcaaatc gttatcactt gccatcgttc atattatttt taagtgttgg cgtgataggc
     4801 tcttttctaa catcagattt atttaattta tacgtcatgt ttgaaattat gttactagcg
     4861 tcatttgtac tcattacact tggacaatct gtagaacaat tacgtgctgc aattatttat
     4921 gttgtcttga atattattgg ttcatggcta ttcttattag gtataggttt actttataaa
     4981 acagtaggta cattaaactt ttcacatatt gcaatgcgtt tgaatgacat gggagaaaat
     5041 cgcactgtta caatgatttc attaatcttc ttagtcgcat ttagtgcgaa agcagcgctg
     5101 gtccttttta tgtggctacc caaagcctac gctgtgttaa atactgagct tgcagcatta
     5161 tttgcagcgt taatgaccaa agtaggggcc tatgcattaa ttcgattctt cactttacta
     5221 tttgatcaac ataatgatct catacatcca ttgctagcaa ctatggctgc tataactatg
     5281 gtcatcggcg ctataggtgt cattgcttat aaagatatta aaaagattgc agcttaccaa
     5341 gtcataatct caataggatt tatcatttta ggtttaggaa caaacacgtt tgcaggtatt
     5401 aatggtgcaa tattttattt ggtaaatgac attgttgtaa aaacattgct attttttatt
     5461 attggtagtt tagtttacat tacaggctat cgacaatatc aatatttgaa tggcttagct
     5521 aaaaaagaac ctttatttgg agttgcgttt attataatga tttttgctat tggcggcgtg
     5581 cctccattta gtggctttcc ggggaaagta cttattttcc aaggtgcatt gcaaaatggc
     5641 aattatattg gactagcgtt aatgattatt actagtctaa ttgcaatgta cagtttattt
     5701 aggatacttt tttatatgta ttttggagat aaagatgggg aggaagttaa ttttaagaaa
     5761 atcccgctat atcgaaaaag aattttaagt attttagtag ttgtggttat cgcaatcgga
     5821 attgctgcac ctgttgtgtt aaatgttaca agtgatgcaa ctgagttgaa cacgagtgat
     5881 caattatatc aaaaacttgt aaatccgcat ttgaaaggag aggactaaat gaatcaaata
     5941 gttttaaata ttatcattgc attcttatgg gtattatttc aagatgaaga tcattttaaa
     6001 ttctcgactt tcttttctgg atatctaatt ggtttaattg tcatttatat attacacagg
     6061 tttttcagcg atgatt
//
LOCUS       DAXWFK010000148         5994 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.149, whole genome shotgun sequence.
ACCESSION   DAXWFK010000148 DAXWFK010000000
VERSION     DAXWFK010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5994
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.149"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            308..1228
                     /gene="mvk"
                     /locus_tag="ACGEXS_002166"
     CDS             308..1228
                     /gene="mvk"
                     /locus_tag="ACGEXS_002166"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFF8013157.1"
                     /translation="MTRKGYGESTGKIILIGEHAVTFGEPAIAVPFNAGKIKVLIEAL
                     ESGNYSSIKSDVYDGMLYDAPDHLKSLVNRFVELNNITEPLAVTIQTNLPPSRGLGSS
                     AAVAVAFVRASYDFLGKSLTKEELIEKANWAEQIAHGKPSGIDTQTIVSGKPVWFQKG
                     HAETLKTLSLDGYMVVIDTGVKGSTRQAVEDVHKLCEDPQYMSHVKHIGKLVLRASDV
                     IEHHNFEALADIFNECHADLKALTVSHDKIEQLMKIGKENGAIAGKLTGAGRGGSMLL
                     LAKDLPTAKNIVKAVEKAGAAHTWIENLGG"
     gene            1233..2216
                     /gene="mvaD"
                     /locus_tag="ACGEXS_002167"
     CDS             1233..2216
                     /gene="mvaD"
                     /locus_tag="ACGEXS_002167"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFF8013158.1"
                     /translation="MIKSGKARAHTNIALIKYWGKKDEALIIPMNNSISVTLEKFYTE
                     TKVTFNDQLTQDQFWLNGEKVSGKELEKISKYMDIVRNRAGIDWYAEIESDNFVPTAA
                     GLASSASAYAALAAACNQALDMQLSDKDLSRLARIGSGSASRSIYGGFAEWEKGYSDE
                     TSYAVPLESNHFEDDLAMIFVVINQHSKKVPSRYGMSLTRNTSRFYQYWLDHIDEDLA
                     EAKAAIQDKDFKRLGEVIEENGLRMHATNLGSTPPFTYLVQESYDVMALVHECREAGY
                     PCYFTMDAGPNVKILVEKKNKQQIIDKLLTQFDNNQIIDSDIIATGIEIIE"
     gene            2229..3305
                     /locus_tag="ACGEXS_002168"
     CDS             2229..3305
                     /locus_tag="ACGEXS_002168"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFF8013159.1"
                     /translation="MIQVKAPGKLYIAGEYAVTEPGYKSVLIALDRFVTATIEEADQY
                     KGTIHSKALHHNPVTFSRDEDSIVISDPHAAKQLNYVVTAIEIFEQYAKSCDIAMKHF
                     HLTIDSNLDDSNGHKYGLGSSAAVLVSVIKVLNEFYDMKLSNLYIYKLAVIANMKLQS
                     LSSCGDIAVSVYSGWLAYSTFDHEWVKHQIEDTTVEEVLIKNWPGLHIEPLQAPENME
                     VLIGWTGSPASSPHFVSEVKRLKSDPSFYGDFLEDSHRCVEKLIHAFKTNNIKGVQKM
                     VRQNRTIIQRMDKEATVDIETEKLKYLCDIAEKYHGASKTSGAGGGDCGITIINKDVD
                     KEKIYDEWTKHGIKPLKFNIYHGQ"
     gene            3482..3823
                     /locus_tag="ACGEXS_002169"
     CDS             3482..3823
                     /locus_tag="ACGEXS_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HFF8013160.1"
                     /translation="MKNTFLICDECQAVNIRTLQKKLEKLDPDAEIVIGCQSYCGPGR
                     RKTFTFVNNRPLAALTEEELIEKVSQQLKKPRDPEEEERLRKRHEERKRRKEEQDRKL
                     KEKLEKRKAQQ"
     gene            complement(3875..5197)
                     /gene="merA"
                     /locus_tag="ACGEXS_002170"
     CDS             complement(3875..5197)
                     /gene="merA"
                     /locus_tag="ACGEXS_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothiocyanous acid reductase MerA"
                     /protein_id="HFF8013161.1"
                     /translation="MKTYDLIVIGFGKAGKTLAKYAASTGQHVAVIEQSPKMYGGTCI
                     NIGCIPSKTLVHDGLEGKSFEASYNRKNDVVNALNNKNYHLLADDNNIDVLDFKAQFK
                     SNTEVNLLDQHDDIVDSITAPHIIINTGATSVIPNIKGLDQAKHVFDSTGLLNISYQP
                     KHLVIVGGGYIALEFASMFANLGSKVTVLERGESFMPREDQDVVAYGITDLENKGIAL
                     HTNVETTELSSDNHHTTVHTNVGNFEADAVLLAIGRKPNTDLALENTDIELGDRGEIK
                     VNAHLQTTVPHIYAAGDVKGGLQFTYISLDDYRIIKSALYGNQSRTTDNRGSVPYTVF
                     IDPPLSRVGLTSKEAAAQHYDYTEHQLLVSAIPRHKINNDPRGLFKVVINNENNMILG
                     ATLYGKQSEELINIIKLAIDQNIPYTVLRDNIYTHPTMAESFNDLFNF"
     gene            complement(5184..5624)
                     /gene="hypR"
                     /locus_tag="ACGEXS_002171"
     CDS             complement(5184..5624)
                     /gene="hypR"
                     /locus_tag="ACGEXS_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional regulator HypR"
                     /protein_id="HFF8013162.1"
                     /translation="MNLEFNIAVHVLAFLTKHHSEKFNSSSLAELTCLNPVQLRRVTT
                     QLVDLKMIDTIRGKDGGYLANDQSADVSLATLYKHFVLEKEHHTRLFTGDEGSHCQIA
                     RNIATTMSHYQQDEQNIIINFYNGKTIKDVIEDIQKEDLCHENI"
     gene            5682..5783
                     /locus_tag="ACGEXS_002172"
     CDS             5682..5783
                     /locus_tag="ACGEXS_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013163.1"
                     /translation="MCQYLCLKSVELGVVIVMIYCLKVDALTVRLHN"
BASE COUNT         2073 a          870 c         1179 g         1872 t
ORIGIN      
        1 attatttgta gtattatagt tagggtggaa aattataaat tgtagggcta ttttataaaa
       61 agataaagaa tctgaaatat cactcgataa gatttaaact ttgaagtaaa cgaaagttag
      121 ttttatacta atcaagttaa gtcaaatata aattcaaaat aaagattata cataatatga
      181 ataatgtgag ataaagactg caatgaaatt taacgcgaat gcgtatgcaa cttagaatat
      241 gatgtctttt actaataaat aaaaataatg aatcaagtat aggataaata taaaaggtga
      301 tattgacatg acaagaaaag gatatgggga atcgacaggt aagattattt taataggaga
      361 acatgctgtt acatttggag agcctgctat tgcagtaccg tttaacgcag gtaaaatcaa
      421 agttttaata gaagccttag agagcgggaa ctattcgtct attaaaagcg atgtttacga
      481 tggtatgtta tatgatgcgc ctgaccatct taagtctttg gtgaaccgtt ttgtagaatt
      541 aaataatatt acagagccgc tagcagtaac gatccaaacg aatttaccac catcacgtgg
      601 attaggatcg agtgcagctg tcgcggttgc ttttgttcgt gcaagttatg attttttagg
      661 gaaatcatta acgaaagaag aactcattga aaaggctaat tgggcagagc aaattgcaca
      721 tggtaaacca agtggtattg atacgcaaac gattgtatca ggcaaaccag tttggttcca
      781 aaaaggtcat gctgaaacgt tgaaaacgtt aagtttagac ggctatatgg ttgttataga
      841 tactggtgtg aaaggttcaa caagacaagc agtagaagat gttcataaac tttgtgagga
      901 ccctcagtac atgtcacatg taaaacatat cggtaagtta gttttacgtg cgagtgatgt
      961 gattgaacat cataactttg aagccttagc ggatattttt aatgaatgtc atgcggattt
     1021 aaaggcgttg acagttagtc atgataaaat agaacaatta atgaaaattg gtaaagaaaa
     1081 tggtgcgatt gctggaaaac ttactggcgc tggtcgtggt ggaagtatgt tattgcttgc
     1141 caaagattta ccaacagcga aaaatattgt aaaagctgta gaaaaagctg gtgcagcaca
     1201 tacttggatt gagaatttag gaggttaatg cgttgattaa aagtggcaaa gcacgtgcac
     1261 atacgaatat tgcacttata aaatattggg gtaaaaaaga tgaagcacta atcattccaa
     1321 tgaataatag catatctgtt acattagaaa aattttacac tgaaacgaaa gtcactttta
     1381 acgaccagtt aacacaggat caattttggt tgaatggtga aaaggttagt ggcaaagaat
     1441 tagagaaaat ttcaaaatat atggatattg tcagaaatag agctggcatc gattggtatg
     1501 cagaaattga aagcgacaat tttgtaccaa cagcagcagg gttggcttca tcggcaagcg
     1561 catatgcagc tttagcagca gcttgtaatc aagcgctaga catgcagctg tcagataagg
     1621 atttatcgag attggcgcga attggttcgg gttctgcgtc gcgtagtatt tatggtggat
     1681 ttgcagaatg ggaaaaaggg tatagtgatg agacgtcata tgccgttcca cttgaatcga
     1741 atcattttga agatgacctt gccatgatat ttgttgtgat taatcaacat tctaaaaagg
     1801 tacctagtcg atatggtatg tcattgacac gaaacacatc aaggttttat caatattggt
     1861 tagatcatat tgatgaagat ttagctgaag caaaagcagc gattcaagac aaagatttta
     1921 aacgccttgg tgaagtaatt gaagaaaatg gtttgcgtat gcatgccacg aatctaggat
     1981 caacaccgcc gttcacatat cttgtgcaag aaagttatga tgtcatggcg cttgttcacg
     2041 aatgccgaga agcggggtat ccgtgttatt ttacaatgga tgcgggacct aatgtgaaaa
     2101 tacttgtaga aaagaaaaac aagcaacaga ttatagataa attattaaca cagtttgata
     2161 ataaccaaat tattgatagt gacattattg ccacaggaat tgaaataatt gagtaaggaa
     2221 gagataaaat gattcaggtc aaagcacccg gaaaacttta tattgctgga gaatatgctg
     2281 taacagaacc aggatataaa tctgtactta ttgcgttaga tcgttttgta actgctacta
     2341 ttgaagaagc agaccaatat aaaggtacca ttcattcaaa agcattacat cataacccag
     2401 ttacatttag tagagatgaa gatagtattg tcatttcaga tccacatgca gcaaaacaat
     2461 taaattatgt ggtcacagct attgaaatat ttgaacaata cgcgaaaagt tgcgatatag
     2521 cgatgaagca ttttcatctg actattgata gtaatttaga tgattcaaat ggtcataaat
     2581 atggattagg ttcaagtgca gcagtacttg tgtcagttat aaaagtatta aatgaatttt
     2641 atgatatgaa gttatctaat ttatacattt ataaactagc agtgattgca aatatgaagt
     2701 tacaaagttt aagttcatgc ggagatattg ctgtgagtgt atatagtgga tggctagcgt
     2761 atagtacttt tgatcatgaa tgggttaagc atcaaattga agatactacg gttgaagaag
     2821 ttttaatcaa aaactggcct ggattgcaca tcgaaccatt acaagcacct gaaaatatgg
     2881 aagtacttat cggttggact ggctcaccgg cgtcatcacc acactttgtt agcgaagtga
     2941 aacgtttgaa atcagatcct tcattttacg gtgacttctt agaagattca catcgttgtg
     3001 ttgaaaaact tattcatgct tttaaaacaa ataacattaa aggtgtgcaa aagatggtgc
     3061 gtcagaatcg tacaattatt caacgtatgg ataaagaagc tacagttgat atagaaactg
     3121 aaaagctaaa atatttgtgt gatattgctg aaaagtatca cggcgcatct aaaacatcag
     3181 gcgctggtgg tggagactgt ggtattacaa ttatcaataa agatgtagat aaagaaaaaa
     3241 tttatgatga atggacaaaa catggtatta aaccattaaa atttaatatt tatcatgggc
     3301 aataaatgat ttgatagggg agcaatcatc taatttaatt agaagataaa tgctccattt
     3361 tttattgtca gccgtgttac gtatcttgaa aaaaatatta aaaagagtaa aatagataga
     3421 gttaacgaaa aaattaatga aatcgacgat atagatatga taaagaaagg tgggtagcaa
     3481 tatgaaaaat acattcctta tttgtgatga atgtcaggca gtcaatataa gaacgttaca
     3541 aaagaagttg gaaaaattag atcccgatgc tgaaatcgtg ataggttgtc aatcttattg
     3601 tggacctgga cgccgaaaaa cattcacttt tgttaataac cgcccactgg ctgcgcttac
     3661 tgaagaagaa ttaatcgaaa aagtttctca acaattaaag aaaccacgtg atcctgaaga
     3721 agaagagcgt ttaagaaaac gacatgaaga acgtaaacgt cgtaaagaag aacaagatag
     3781 aaagcttaaa gaaaaattag aaaagcgaaa agcacaacaa taaagcctga tggcagcatc
     3841 attcaatgcg tgccaccagg tttttatgtt ttgtctagaa attaaataaa tcattaaatg
     3901 attcggccat cgtaggatgc gtataaatat tatctcgtaa tacggtatat ggaatgtttt
     3961 gatcaatcgc aagtttaatt atattaatta attcttcaga ttgcttacca tataatgtag
     4021 cacctaaaat catattattt tcattattaa tgactacttt aaataaacct cttggatcat
     4081 tgttaatttt atgacgaggt atagcactta ctaaaagttg atgttcagtg taatcataat
     4141 gttgagcggc agcttcttta ctagttaatc caacacgtga taatggtgga tctataaata
     4201 ctgtataagg cacgctgcct ctattgtcag tcgtacgtga ctgattacca tataacgctg
     4261 atttgataat tcgataatca tctaaagata tatacgtaaa ttgaagtccg cctttaacat
     4321 cacctgcagc ataaatatgc ggcacagttg tttgaagatg agcattgact ttaatttcgc
     4381 ctctgtcgcc taattcgata tcagtatttt ctaaagctaa atccgtattc ggtttgcgcc
     4441 cgatagccaa aagtactgca tcagcctcaa agttaccaac gttggtatgg actgttgtat
     4501 gatgattgtc agatgacaat tcagtcgttt caacatttgt atgcaatgca atgcctttat
     4561 tttctaagtc agtaatacca tatgcaacga catcttgatc ttcgcgtggc ataaatgatt
     4621 cgccacgttc taatactgtt accttactac ctaaattcgc aaacattgaa gcaaattcta
     4681 aggcgatata accgccacct acaataacga ggtgcttagg ttgatagcta atgtttaata
     4741 aacctgtcga atcgaagacg tgtttagctt gatcaaggcc tttaatgtta ggaatgacag
     4801 aggtagcacc ggtattaata atgatatgag gtgcagtaat actatcgacg atatcgtcat
     4861 gttgatctaa taaattcact tcagtattag atttaaactg cgctttaaaa tccagtacat
     4921 caatgttgtt atcgtctgct aataagtggt aatttttatt gtttagcgca ttgacaacat
     4981 cgtttttacg gttataactt gcttcaaaag atttgccttc taatccatca tgtacaagtg
     5041 tcttcgaagg tatacatcct atgtttatac aagtgcctcc atacattttc ggagattgtt
     5101 cgataactgc gacgtgttga cctgttgatg cagcgtattt cgctaaagtt ttaccagctt
     5161 tcccaaatcc tattacaatt aaatcatatg ttttcatgac ataaatcctc cttttgaatg
     5221 tcttcaatga catctttgat tgtttttcca ttataaaaat taataatgat attctgttcg
     5281 tcttgctgat aatgtgacat ggtagttgca atattacgag caatttgaca gtgactgcct
     5341 tcgtcgccag taaatagacg tgtgtggtgt tctttctcta agacaaaatg tttatataat
     5401 gttgctagag agacatcagc actttgatca tttgctaaat aaccgccatc tttacctcgt
     5461 attgtgtcaa tcatttttaa atcgacaagt tgagtcgtca cgcgtcgtaa ttgaacagga
     5521 tttaaacaag ttaattctgc taatgaacta ctattgaatt tttctgaatg atgcttagtt
     5581 aaaaaagcta atacatgcac ggcaatgtta aattctaaat tcaatgtttc tcacctcata
     5641 attgtaactg tattagttac aattaaaaat cattttgctc aatgtgtcaa tacttatgtt
     5701 tgaaaagtgt tgaattaggt gtggttattg tgatgattta ttgtttgaaa gttgatgcat
     5761 taacggtgag attgcacaat tgaaatttta attatgtatg aactgataga tatgaaatga
     5821 atattgaaaa tgagagcaat aaaattttga atgatgatgt tgtgtgattg tataaactta
     5881 ttcgtatttt agatacgttt tgatatttcg catgatgagg aaatactttt aacaactgat
     5941 gacatatttt aatagtgaat atattttgag atattaatat atttaatgtt aagg
//
LOCUS       DAXWFK010000149         5977 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.150, whole genome shotgun sequence.
ACCESSION   DAXWFK010000149 DAXWFK010000000
VERSION     DAXWFK010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5977
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.150"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            128..892
                     /locus_tag="ACGEXS_002173"
     CDS             128..892
                     /locus_tag="ACGEXS_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFF8013164.1"
                     /translation="MDINSEEYKQEVLIKDVVMLAARILLESGAEGTRVEDTMTRIAK
                     KLGYSESNSFVTNTVIQFTLHSESFPRIFRITSRDTNLIKISQANKISRQITNNEISL
                     AEAKTQLEKIYVAKRDSSLPFKGFAAAMIAMSFLYLQGGRLIDVLTAILAGSLGYLVT
                     EILDRKLHAQFIPEFIGSLVIGIIAVIGHTLIPTGDLATIIIAAVMPIVPGVLITNAI
                     QDLFGGHMLMFTTKSLEALVTAFGIGAGVGSVLILV"
     gene            905..1357
                     /locus_tag="ACGEXS_002174"
     CDS             905..1357
                     /locus_tag="ACGEXS_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFF8013165.1"
                     /translation="MFWILNFIFSFLASMFFCVIFDAPRKLYLSCGFVGTCGWMVYTL
                     FFNGFNVHTIYSSFFGSLALGLLSHYMARKQKEPAIIFMVTGIIPLVPGGLAYDATKN
                     LVLLNFSTAINTMLEVTLIAGAIALGLLFADQISKLIVSGFVKSFKRL"
     gene            complement(1499..2983)
                     /locus_tag="ACGEXS_002175"
     CDS             complement(1499..2983)
                     /locus_tag="ACGEXS_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFF8013166.1"
                     /translation="MEKKNKQIDRGDLKQNLSEKFVWAIAYGSCIGWGAFILPGDWIK
                     QSGPIAASIGIVIGALLMILIAVSYGALVERFPVSGGAFAFSFLSFGRYVSFFSSWFL
                     TFGYVCVVALNATAFSLLVKFLLPDVLNNGKLYTIAGWDVYITEIIIATVLLLVFMLV
                     TIRGASVSGSLQYYFCVAMVIVVLLMFFGSFFGNNFALENLQPLAEPSKGWLVSIVVI
                     VSVAPWAYVGFDNIPQTAEEFNFAPNKTFKLIVYSLLAASLTYVVMILYTGWLSTSHQ
                     SLNGQLWLTGAVTQTAFGYIGLGVLAIAIMMGIFTGLNGFLMSSSRLLFSMGRSGIMP
                     TMFSKLHSKYKTPYVAIIFLVGVSLIAPWLGRTALTWIVDMSSTGVSIAYFITCLSAA
                     KLFSYNKQSNTYAPVYKTFAIIGSFVSFIFLALLLVPGSPAALTAPSYIALLGWLIIG
                     LIFFVIRYPKLKNMDNDELSRLILNRSENEVDDMIEEPEKEKTK"
     gene            complement(3104..3472)
                     /locus_tag="ACGEXS_002176"
     CDS             complement(3104..3472)
                     /locus_tag="ACGEXS_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFF8013167.1"
                     /translation="MKLFIILGALNAMMAVGTGAFGAHGLQGKISDHYLSVWEKATTY
                     QMYHGLALLIIGVISGTTSINVNWAGWLIFAGIIFFSGSLYILVLTQIKVLGAITPIG
                     GVLFIIGWIMLIIATFKFAG"
     gene            complement(3605..3985)
                     /locus_tag="ACGEXS_002177"
     CDS             complement(3605..3985)
                     /locus_tag="ACGEXS_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5327 family protein"
                     /protein_id="HFF8013168.1"
                     /translation="MLNRETLIARIEQELVQAEQAQHDHDFEKHMYAIHILTSLYAST
                     SNTPHIGEQQMNRRIANHNQMPQSQITQPTHQVTVAEIEAMGGKVNTHSAHHHNKSYS
                     QPSNQQQRLATDDDIGNGESIFDF"
     gene            complement(3986..4642)
                     /locus_tag="ACGEXS_002178"
     CDS             complement(3986..4642)
                     /locus_tag="ACGEXS_002178"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFF8013169.1"
                     /translation="MEWSQIFHDITTKHDFKAMHDFLEKEYSTAIVYPDRENIYQAFD
                     LTPFENIKVVILGQDPYHGPNQAHGLAFSVQPNAKFPPSLRNMYKELADDIGCVRQTP
                     HLQDWAREGVLLLNTVLTVRQGEANSHRDIGWETFTDEIIKAVSDYKEHVVFILWGKP
                     AQQKIKLIDTSKHCIIKSVHPSPLSAYRGFFGSKPYSKANAYLESVGKSPINWCESEA
                     "
     gene            4826..5656
                     /gene="thiD"
                     /locus_tag="ACGEXS_002179"
     CDS             4826..5656
                     /gene="thiD"
                     /locus_tag="ACGEXS_002179"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFF8013170.1"
                     /translation="MALKKVLTIAGSDTSAGAGMQADLKTFQELDTYGMVALTAIVTM
                     DKDTWSHDVTPLPMDVFEKQLETALSIGPDAIKTGMLGTEEIIKRAGEVYEASNAQYF
                     VVDPVMVCKGEDEVLNPGNTEAMIKYLLPKATVVTPNLFEAGQLSGLGKLNSIEDMKK
                     AATIIFDKGAQHVIIKGGKALDQDKSYDLYYDGQTFYQLTTDMFQQSYNHGAGCTFAA
                     ATTAYLANGKSPKEAVISAKAFVASAIKNGWKMNDFVGPVDHGAYNRIEHIDVEVTEV
                     "
BASE COUNT         1963 a         1076 c          975 g         1963 t
ORIGIN      
        1 tattattaca atagtatcaa atattagttt tttaaaatca ataacaactt atcaaaaagc
       61 tcatgtggtt attttatagt gtataaacta taatgagtat taaattctta taaacaatgg
      121 tgatgaaatg gacataaatt cagaagaata caaacaagag gtacttatca aagacgttgt
      181 catgcttgct gctcgcatac tattagaatc tggtgcagaa ggtacgcgtg tagaagatac
      241 catgacacgt attgcaaaaa aacttggtta cagtgaaagt aacagctttg ttacaaacac
      301 tgtcatccag tttacgttac attcggaatc gtttcctaga atatttagaa ttacctctcg
      361 agatacaaac ttaataaaaa tttctcaagc taataaaatt tcgcgtcaaa ttacaaacaa
      421 tgaaatttct ttagccgaag caaaaacgca acttgaaaaa atatatgttg ctaagcgtga
      481 cagcagtctt ccctttaaag gttttgctgc agcaatgatt gcaatgagtt tcttatattt
      541 acaaggtggt agattgattg atgttttaac tgcgatatta gcaggtagtc taggatacct
      601 agtcactgag attttagatc gtaagttaca cgcacagttt atcccagaat tcattggttc
      661 attagttatt gggattatcg ccgttattgg acatacactt attccaacag gtgacttggc
      721 aactattatc attgcggcag tcatgcctat tgttcctggt gtattaataa caaacgcaat
      781 acaagattta tttggtggac acatgttgat gttcacaacg aaatcattag aagcattggt
      841 tactgcgttt ggcatcggtg ctggcgttgg tagcgtatta attttagtat aggagtatca
      901 gactatgttt tggatcttaa actttatctt tagcttttta gcttcaatgt tcttctgcgt
      961 catttttgat gcacctagaa aattatatct atcatgtggt ttcgttggta cgtgtggatg
     1021 gatggtttac accttattct tcaacggctt taatgtgcac actatatact ctagtttctt
     1081 tggtagttta gcattaggct tgttaagtca ttatatggct cgtaaacaaa aagaacctgc
     1141 catcattttt atggtaacgg gtatcattcc attagtacct ggtggcttag catacgatgc
     1201 tacaaaaaat ttagtcttat taaatttcag tacagcaatc aataccatgc tagaggttac
     1261 acttattgca ggcgccatcg cattaggttt attattcgcc gaccaaattt ccaaattaat
     1321 tgtttctggg ttcgtgaaat cttttaaacg attataaata tttgttagaa atggctcagc
     1381 cctaactctt agcaattata tataaggttc agctgcctcg tcttaaatgt caatttataa
     1441 tacaaaaaat cgcacgatac gcctccgaat gaagaaggtt tattgtgcga ttcttttatt
     1501 atttagtttt ttctttttca ggttcttcaa tcatatcatc aacttcattt tcacttctat
     1561 ttaaaatcaa gcgacttaat tcatcattat ccatattttt caatttagga tatcgaatca
     1621 caaagaatat taaaccgatg attaaccatc caagtaatgc aatataagac ggtgcagtca
     1681 gtgctgcagg agaacctggc actaataaca acgctaagaa aatgaatgat acaaatgagc
     1741 cgataatagc aaacgttttg taaaccggtg catacgtatt actttgtttg ttataactga
     1801 ataatttcgc tgcagacaaa catgtaataa agtaggcaat ggatacacca gtagatgaca
     1861 tatctacaat ccaagtcaat gcagttcttc ctagccaagg tgcaattaac gacactccta
     1921 ctaggaatat gattgcgaca tatggtgttt tgtatttact atgtaattta ctaaacattg
     1981 ttggcataat acctgaacgt cccatagaaa ataacaagcg acttgaactc atcaagaatc
     2041 catttaaacc agtaaatata cccatcataa ttgcaattgc taatacacct aatccaatat
     2101 aaccaaatgc tgtttgtgta acagcacctg ttaaccacaa ctgcccattt aaactttgat
     2161 gacttgttga taaccaacca gtgtataaaa tcatgacaac ataagttaat gatgctgcta
     2221 ataaactgta cacgataagc ttaaatgtct tgtttggtgc aaagttaaac tcttctgctg
     2281 tttgtggaat attatcaaat ccaacatatg cccatggtgc cacggataca ataaccacaa
     2341 tagacactaa ccatcctttg ctaggttcag ctaacggttg taaattttca agtgcaaaat
     2401 tattaccaaa gaatgaacca aagaacatca ataatacgac gattaccatc gccacacaga
     2461 aataatattg taatgatcca gatacacttg cgccacgaat cgttactagc atgaatacaa
     2521 gtagtaatac ggtcgcaata atgatttccg taatataaac gtcccagccc gcaatggtgt
     2581 atagtttccc attatttaag acatctggca ataagaattt aactagtaaa ctgaatgcgg
     2641 tcgcatttaa agcaacgaca cagacataac caaaagttaa aaaccatgat gagaagaaac
     2701 tcacatatct gccgaaactt aagaaactaa aggcaaacgc gccccctgat actggaaatc
     2761 tctctactaa tgcgccataa ctaaccgcaa ttaatatcat taataatgca ccaataacta
     2821 taccaattga tgctgcaatc ggacctgact gcttaatcca gtctcctggt aagatgaatg
     2881 cgccccatcc gatacatgaa ccatatgcaa tcgcccatac aaacttttca gataggtttt
     2941 gttttaaatc gcctctatct atttgcttat tctttttttc cataaataaa aactcacctc
     3001 gaaggtattc tatacccaaa agatgagttt gtaactcttt ttcttgatta tttttattaa
     3061 aaatttaatg tttagttaca taggtaatct aaagttttaa aatttaacca gcaaatttga
     3121 atgtcgcaat gattaacatt atccatccaa tgatgaacaa tacgccacca attggcgtaa
     3181 tcgcacctaa aactttaatt tgagttaata ctaaaatata taatgatcca ctaaagaaaa
     3241 taataccagc aaatattaac cagccagccc agttaacatt gattgaagtt gtaccactaa
     3301 ttacacctat aattaataat gctaagccat ggtacatttg atacgtcgtt gctttttccc
     3361 atactgataa atagtgatca cttatttttc cttgtaaacc atgcgcacca aatgcacctg
     3421 taccgacagc catcatcgcg tttaatgcac ctaaaataat aaataatttc attgctacct
     3481 ctccttatat aattcagtat taataaatgt ttaaatccga acactcgaat aaaagtatat
     3541 gcgtcttgat tattaaccta catacttaac aagctattaa gtaattatgt ttcattgttg
     3601 ctttttaaaa atcaaatatg gattcaccat tgccaatgtc atcatctgtc gctaatcttt
     3661 gttgttggtt tgaaggttgt gaatatgact tattatgatg atgtgctgaa tgcgtattta
     3721 ctttaccacc catcgcttca atttcagcaa ctgtcacttg atgagttggc tgcgttattt
     3781 gtgattgtgg catttgatta tggttagcaa tacgacgatt catttgttgt tcaccaatat
     3841 gtggtgtatt tgatgttgaa gcatataaag atgttaatat atgtatggca tacatatgtt
     3901 tttcaaagtc atggtcatgc tgtgcctgct ctgcttgtac taattcttgc tcaattcgtg
     3961 ctattaaagt ttctctattc aacatctacg cctcactttc acaccaatta attggtgatt
     4021 ttcctactga ctctaaatag gcattcgctt tggaatacgg ttttgatcca aagaatcctc
     4081 tatatgcaga cagtggacta ggatgcactg attttataat acaatgttta gatgtatcga
     4141 taagctttat tttttgctgt gcaggtttcc cccacaaaat aaagacaaca tgttctttat
     4201 aatcagacac tgctttaata atttcatcag taaatgtttc ccaaccaata tcacgatgag
     4261 aatttgcttc accctgtctt acggttaaaa ctgtattcaa taacaagacg ccttctcttg
     4321 cccaatcttg taaatgcggt gtttgtctaa cgcatccaat atcatctgct aattctttat
     4381 acatattacg taaagatgga gggaattttg cgttaggttg cactgaaaat gctaatccat
     4441 gtgcttggtt tggaccatga tacgggtctt gtcctaatat aacaactttg atattttcaa
     4501 acggtgttaa atcaaacgct tgatatatat tttccctatc agggtatacg attgcagtcg
     4561 aatattcttt ttctaaaaaa tcatgcatag ctttaaagtc atgtttcgtt gttatgtcat
     4621 gaaaaatttg cgaccattcc attcaatctc acctcaggac atattttaac atatcacact
     4681 tatctaaagt gaatgacatt ttcaaaagtt acaatagtat gaatgattca tttcaacaat
     4741 catcaaatat tgaatgctta atatttttca attaggttaa aaatgatatg atgaatacaa
     4801 tattattatg taggagtgaa gctcaatggc tttaaagaaa gttttaacaa ttgccggttc
     4861 tgacacaagt gctggcgcag gtatgcaagc agatttgaaa acgttccaag aattagatac
     4921 gtatggcatg gtcgctttaa ctgccatcgt tactatggat aaagatacat ggtcacacga
     4981 tgttacacca ttaccaatgg atgtatttga aaaacaactt gaaacggctt tatcaatcgg
     5041 acctgatgct attaaaacag gtatgttagg tactgaggag ataatcaaac gtgctggaga
     5101 agtatatgag gcatctaatg cacaatattt tgtagtagat cctgtcatgg tttgtaaagg
     5161 cgaagatgaa gtgcttaatc ctggaaatac tgaggcgatg attaaatatt tactacctaa
     5221 agcaacggta gtgacaccaa acttatttga agcgggtcaa ttatcaggat taggtaaatt
     5281 aaattcaatt gaagatatga aaaaggctgc aacaattatc tttgataaag gcgcacagca
     5341 tgtcatcatt aaaggtggca aagctttaga tcaagataaa tcgtatgact tatactatga
     5401 tggtcaaaca ttttatcaac taacaacaga catgttccaa caaagttata accatggtgc
     5461 tggttgtaca tttgctgcgg caacaactgc atatttagct aacggtaagt caccgaaaga
     5521 agctgtgatt agcgcgaaag ctttcgtcgc ttctgctatt aaaaacggtt ggaaaatgaa
     5581 tgattttgtt ggtcctgtgg atcacggtgc atacaaccgt attgaacata tcgatgttga
     5641 agtaacagag gtttaacgat taggcggttc atctataaat caacattatt agccacacca
     5701 cttaaattgg gtatgaactc aatttatgtg atgtggttat ttttttataa tcatcatgct
     5761 ctgtatataa tttaaatttg aaagttacat ataagcgtta ttagctttta ttgaaaattt
     5821 catacatatc gaacaataca tacaaatatt tcctatacat ctacatctat gactaaataa
     5881 ccctttattt tcaagatgaa cttaaactca tctatatgct caatacctct atcattatca
     5941 ttcttttgcg atagatgcct ctctaatcat atatgtt
//
LOCUS       DAXWFK010000150         5850 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.151, whole genome shotgun sequence.
ACCESSION   DAXWFK010000150 DAXWFK010000000
VERSION     DAXWFK010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5850
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.151"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            307..1707
                     /locus_tag="ACGEXS_002180"
     CDS             307..1707
                     /locus_tag="ACGEXS_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8013171.1"
                     /translation="MDFNKENINMVDAKKAKKTVVATGIGNAMEWFDFGVYAYTTAYI
                     GANFFSPVENADIRQMLTFAALAIAFLLRPIGGVVFGIIGDKYGRKVVLTSTIILMAF
                     STLTIGLLPSYDQIGLWAPILLLLARVLQGFSTGGEYAGAMTYVAESSPDKRRNSLGS
                     GLEIGTLSGYIAASIMIAVLTFFLTDEQMASFGWRIPFLLGLFLGLFGLYLRRKLEES
                     PVFENDVATQPERDNINFLQIIRFYYKDIFVCFVAVVFFNVTNYMVTAYLPTYLEQVI
                     KLDATTTSVLITCVMAIMIPLALMFGKLADKIGEKKVFLIGTGGLTLFSIIAFMLLHS
                     QSFVVIVIGIFILGFFLSTYEATMPGSLPTMFYSHIRYRTLSVTFNISVSIFGGTTPL
                     VATWLVTKTGDPLAPAYYLTAISVIGFLVITFLHLSTAGKSLKGSYPNVDNEQDRAYY
                     AEHPKEALWWVKERKN"
     gene            2251..3627
                     /locus_tag="ACGEXS_002181"
     CDS             2251..3627
                     /locus_tag="ACGEXS_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFF8013172.1"
                     /translation="MNVILEQLKTHTQNKPNDIALHIDDETITYSQLNARITSAVESL
                     QKYSLNPVVAINMKSPVQSIICYLALHRLHKVPMMMEGKWQSTIHRQLIEKYGIKDVI
                     GDTGLMQNIDSPMFIDSTQLQHYPNLLHIGFTSGTTGLPKAYYRDEDSWLASFEVNEM
                     LMLKNENAIAAPGPLSHSLTLYALLFALSSGRTFIGQTTFHPEKLLNQCHKISSYKVA
                     MFLVPTMIKSLLLVYNNEHTIQSFFSSGDKLHSSIFKKIKNQANDINLIEFFGTSETS
                     FISYNLNQQAPVESVGVLFPNVELKTTNHDHNGIGTICIKSNMMFSGYVSEQCINNDE
                     WFVTNDNGYVKEQYLYLTGRQQDMLIIGGQNIYPAHVERLLTQSSSIDEAIIIGIPNE
                     RFGQIGVLLYSGDVTLTHKNVKQFLKKKVKRYEIPSMIHHVEKMYYTASGKIAREKMM
                     SMYLRGEL"
     gene            3629..4768
                     /locus_tag="ACGEXS_002182"
     CDS             3629..4768
                     /locus_tag="ACGEXS_002182"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HFF8013173.1"
                     /translation="MNQAVIVAAKRTAFGKYGGTLKHLEPEQLLKPLFQHFKEKYPEV
                     ISKIDDVVLGNVVGNGGNIARKALLEAGLKDSIPGVTIDRQCGSGLESVQYACRMIQA
                     GAGKVYIAGGVESTSRAPWKIKRPHSVYETALPEFYERASFAPEMSDPSMIQGAENVA
                     KMYDVSRELQDEFAYRSHQLTAENVKNGNISQEILPITVKGEIFNTDESLKSHIPKDN
                     FGRFKPVIKGGTVTAANSCMKNDGAVLLLIMEKDMAYELGFEHGLLFKDGVTVGVDSN
                     FPGIGPVPAISNLLKRNQLTIENIEVIEINEAFSAQVVACQQALNISNTQLNIWGGAL
                     ASGHPYGASGAQLVTRLFYMFDKETMIASMGIGGGLGNAALFTRF"
     gene            4743..5108
                     /locus_tag="ACGEXS_002183"
     CDS             4743..5108
                     /locus_tag="ACGEXS_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein VraC"
                     /protein_id="HFF8013174.1"
                     /translation="MQHYLLDSNQRLNVSFSKDSVAAYYQCFNQPYRKEVPPLMCASL
                     WPKFDLFKKYANSELILTKSAINQTQKIEVDTIYVGHLEDIECRQTRNITRYTMALTL
                     TKNDQHVITVTQTFIKAMK"
     gene            5111..5380
                     /locus_tag="ACGEXS_002184"
     CDS             5111..5380
                     /locus_tag="ACGEXS_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013175.1"
                     /translation="MKFNEIWINEYLALVNDDNPIHNEIVPGQLVSQMMLMAMSLETN
                     QCQINYVKPILINENIEFIEQHEHEIIAINDDGEIKIKISLSTKK"
     gene            complement(5581..5748)
                     /gene="vraX"
                     /locus_tag="ACGEXS_002185"
     CDS             complement(5581..5748)
                     /gene="vraX"
                     /locus_tag="ACGEXS_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1q-binding complement inhibitor VraX"
                     /protein_id="HFF8013176.1"
                     /translation="MIIYRQYHHEGAPVYEIITKTFQHVSIKCDDSFSDTEIFKLLSL
                     LQDDIDHMKVS"
BASE COUNT         2034 a          847 c         1077 g         1892 t
ORIGIN      
        1 agtaaattga gtataaacat ttaaatacga tgtcgaaaat ggcgatagca tatcacttac
       61 atgaagttgt gtgctatcgc tatttttagt tataattcca aaaagttaat cgttcgatga
      121 tttaagaatt attattgttt aattcaaatg tatgagggta taaaatcatt gaatttaatt
      181 cgataaagcg aaatttttga acaaacatac ttttgtattt atataaaagt ttaaattctt
      241 ataaatttga caaaactaat taactccgta taattatgaa acatacaaga gggagtgtat
      301 gaattcatgg attttaataa agagaatatt aacatggtgg atgcaaagaa agctaaaaaa
      361 accgttgttg caaccggtat cggtaatgca atggaatggt tcgattttgg tgtctatgca
      421 tatacaactg cgtacattgg agcgaacttc ttctctccag tagagaatgc agacattcga
      481 caaatgttga ctttcgcagc attagccatt gcgtttttat taagaccaat tggtggtgtc
      541 gtatttggta ttattggtga caaatatgga cgtaaagttg tattaacatc tacaattatt
      601 ttaatggcat tttcaacatt aaccattgga ttattgccaa gctatgatca aattggactt
      661 tgggcaccaa tactattatt gcttgcaaga gtactacaag ggttttcaac aggtggagag
      721 tatgcggggg caatgacata tgttgccgaa tcatctccag ataagcgtcg taactcatta
      781 ggtagtggac tagaaattgg gacattatca ggttacatag ctgcttcaat tatgattgct
      841 gtattaacat tctttttaac agatgaacaa atggcatcat ttggttggag aatcccattc
      901 ttactcggtt tattcctagg attattcggc ttatatttac gtcgtaagct ggaagaatca
      961 ccagttttcg aaaatgatgt tgcaacacaa ccagaaagag ataacattaa ctttttacaa
     1021 atcatcagat tttattacaa agatatattt gtatgttttg tagctgttgt attcttcaat
     1081 gttacaaact atatggtaac tgcatattta ccaacctatt tagaacaagt tattaaatta
     1141 gatgcaacga caacaagtgt attaattact tgtgtcatgg caataatgat tccattagca
     1201 ttaatgtttg gtaagttagc ggataaaata ggtgaaaaga aagtatttct aattggtact
     1261 ggtgggctaa cattattcag tatcatcgca tttatgttat tacattcaca atcatttgtt
     1321 gtaatagtaa tcggtatatt tatattagga tttttcttat caacttacga agcgacaatg
     1381 ccagggtcgt taccaacgat gttttacagt catataagat atcgaacttt atcagtaaca
     1441 tttaatatct ctgtttcgat atttggtggt acgacgccat tagttgcaac atggttagtt
     1501 acgaaaactg gagatccatt agcacctgcg tattatttaa cagcaatcag tgttattggc
     1561 tttttagtta ttacattctt acatttaagt acagcaggaa aatctctaaa aggttcgtat
     1621 ccaaatgtag ataacgagca agatagagct tattatgcag aacatccaaa agaagcatta
     1681 tggtgggtta aagaacgtaa gaattagaga ttttaataaa aagtataaat caatcgtata
     1741 taagcacttt aaagctagta ggttctgcta actttaaagt gctttttaaa ttgagaactg
     1801 taattagccg taataaagtt tttgtatata cataaacccc cactgcaatg attatcgcaa
     1861 tgggggaaag aggggactta aagcatatgt ttagctttga atacttaaaa ttctcttgct
     1921 attgaaatgt taggatgtaa atatgtctta gagtattttg tccaacgcaa ttaatattga
     1981 gactctaacc ttcaatatta ttatagagaa cacaaactta aatagattgg gtgacttatt
     2041 tgtgtcagtt attgcgattg cgataacttc ttttctctat atacatatag taacgtctta
     2101 tctaataaaa aacatggtac tacagtatca aatttatcta gggcttaagt ttgattttta
     2161 taataggcag gtttacctga taaaaatact tattcattat ataatgttaa caatatgtat
     2221 tttaaagttt acattgagtg agggatattg atgaacgtaa ttttagaaca gttgaaaaca
     2281 catactcaaa ataaacctaa tgacatagca ttacatatcg atgatgaaac aattacatat
     2341 agtcaactaa atgcccgcat cactagcgca gttgaatctt tgcagaaata ttcacttaac
     2401 cctgtcgttg ctattaatat gaaatcaccg gtgcaaagta ttatttgtta tttagctttg
     2461 catcgtttac ataaagtgcc tatgatgatg gaaggtaaat ggcaaagtac tatacatcgt
     2521 caattgattg aaaaatatgg tattaaagat gtaattggag atacaggtct catgcagaat
     2581 atagactcac cgatgtttat tgattcaacg caattacagc actaccccaa tttattacat
     2641 attggtttta cttcagggac aactggactg ccaaaagcat attatcgtga tgaagattca
     2701 tggttggctt cttttgaagt taatgaaatg ttgatgttaa aaaatgaaaa tgcaatagca
     2761 gcccctggac cactatcgca ctcgttaaca ttatatgcgt tattgtttgc tttaagttcc
     2821 ggtcgtactt ttataggaca gaccactttt catcctgaaa agttacttaa tcaatgtcat
     2881 aaaatatcat catacaaagt tgctatgttt cttgttccaa cgatgattaa atcattattg
     2941 ttagtttaca acaatgaaca tacaatccaa tcatttttta gcagtggaga taagctgcat
     3001 tcttctattt ttaaaaagat aaaaaatcaa gcaaatgaca taaatttgat tgaatttttt
     3061 ggtacatcgg aaaccagttt tatcagctat aacttgaatc agcaagcacc agttgaatca
     3121 gtaggtgtgc tatttccaaa tgtggaattg aaaacaacga atcacgatca caatggtata
     3181 ggaactattt gtataaaaag taatatgatg tttagtggct atgtaagtga acaatgtata
     3241 aataatgatg aatggtttgt tactaatgat aatggctatg taaaagagca gtatttatat
     3301 ttaacgggac gtcaacagga tatgttaatt attggtggtc aaaatatata tccagcacat
     3361 gttgaacgcc ttttaacgca atcttcgagc attgatgaag caattatcat cggtattcca
     3421 aatgagcgtt ttggtcaaat aggcgtattg ctttattctg gtgatgtgac acttacacat
     3481 aaaaatgtaa aacaattttt aaaaaagaaa gtgaaacgct atgaaattcc atcgatgatt
     3541 catcatgtag aaaagatgta ttacactgca agtggtaaaa ttgctagaga aaaaatgatg
     3601 tcgatgtatt tgagaggtga attataatat gaatcaagca gtcatagttg cagctaaacg
     3661 aactgcattt gggaaatatg gtggcacttt aaaacattta gagccagaac aattgcttaa
     3721 acctttattc caacatttta aagagaagta tccagaggta atatctaaaa tagatgatgt
     3781 agttttaggt aatgttgttg ggaatggtgg caatattgca agaaaagcat tgcttgaagc
     3841 ggggcttaaa gattcaatac ctggcgtcac aatcgatcgg caatgtgggt ctggacttga
     3901 aagtgttcaa tatgcatgtc gcatgatcca agccggagct ggcaaggtat atattgcagg
     3961 tggtgttgaa agtacaagtc gagcaccttg gaaaatcaaa cgaccgcatt ctgtgtacga
     4021 aacagcatta cctgagtttt atgagcgtgc atcatttgca cctgaaatga gcgacccatc
     4081 aatgattcaa ggtgctgaaa atgtggccaa gatgtatgat gtttcaagag aattacaaga
     4141 tgaatttgct tatcgaagtc atcaattgac agcggaaaat gtaaagaatg gaaatatttc
     4201 tcaggaaata ttacctataa ccgttaaagg agaaatattc aacactgatg aaagtctaaa
     4261 atcacatatt ccgaaagata actttggccg atttaagccc gtgatcaaag gtgggaccgt
     4321 taccgctgcg aatagttgta tgaaaaatga tggtgcagtt ttattgctta ttatggaaaa
     4381 agatatggca tacgaattag gtttcgagca tggtttatta tttaaagatg gtgttacggt
     4441 aggtgttgat tctaattttc ctggcattgg tccagtacca gccatttcca acttactaaa
     4501 aagaaatcaa ttaacgatag aaaatattga agtcattgaa attaacgaag cgttcagtgc
     4561 acaggtagtt gcctgccaac aagctttaaa tatttcaaat acgcaattaa atatatgggg
     4621 tggtgcatta gcatcaggtc atccatacgg tgcaagcggt gcccaattag tgactcgatt
     4681 attttatatg tttgacaaag agactatgat tgcatctatg gggatagggg gaggtctagg
     4741 aaatgcagca ttatttactc gattctaacc agcgattaaa tgtgtcattt tctaaggata
     4801 gtgtggctgc atattatcag tgttttaacc aaccttatag aaaagaagta ccaccattaa
     4861 tgtgtgcgtc attatggcca aaatttgatt tatttaaaaa atatgcaaat agcgaactga
     4921 ttttaacaaa atcagcaatt aatcaaactc aaaagataga agtagacaca atatatgtag
     4981 ggcatttaga agatattgaa tgccgacaga ctcgcaatat cacacgttat acaatggctt
     5041 taacattaac taaaaatgat caacatgtca taacggttac acaaactttt attaaggcga
     5101 tgaagtagag atgaagttta atgagatatg gataaatgaa tatttggcgc tcgtaaatga
     5161 tgataatcca atacataatg agattgtgcc aggacaatta gtgagtcaaa tgatgctgat
     5221 ggctatgtca ttagagacaa accagtgtca aattaactac gttaaaccta ttttaataaa
     5281 tgaaaatatc gaattcattg aacaacacga acacgaaatt atagcaatta atgacgatgg
     5341 agagattaaa ataaaaattt ctttgagcac aaaaaaataa ccgatattag ctgcatgaac
     5401 gcatattaat taggagatga aaggacagct aatatcagtt atgtattgtt attattattg
     5461 ggaacagaga tgaatatagg ttacgtttct ttctttgcac ggggatgcat taatctaaaa
     5521 taataataac aactatatca atgtttaata aattctggat tattggaacg attagtcaat
     5581 ttaactaact ttcatatgat ctatatcgtc ttgtaataaa gagagcaatt tgaatatttc
     5641 agtatcacta aatgaatcgt cacatttaat tgaaacatgc tgaaacgttt tggttataat
     5701 ttcataaact ggtgcgcctt catggtgata ctgtcgataa ataatcataa cctatattac
     5761 ctcctttgct actctatggt tatattataa ataacatttt tatgtgtgac atcaacctta
     5821 agtatcaact ttttatcaga catagaacgt
//
LOCUS       DAXWFK010000151         5818 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.152, whole genome shotgun sequence.
ACCESSION   DAXWFK010000151 DAXWFK010000000
VERSION     DAXWFK010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5818
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.152"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            143..445
                     /locus_tag="ACGEXS_002186"
     CDS             143..445
                     /locus_tag="ACGEXS_002186"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HFF8013177.1"
                     /translation="MHFTQREQDKLMIVVAAEVARRRKARGLKLNHPEALALISDELL
                     EGARDGKTVAELMSYGRQILNKEDVMDGVEHMITDIEIEATFPDGTKLITVHHPIV"
     gene            459..869
                     /locus_tag="ACGEXS_002187"
     CDS             459..869
                     /locus_tag="ACGEXS_002187"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HFF8013178.1"
                     /translation="MIPGEIITKSTEVEINNHHPETVIEVENTGDRPIQVGSHFHFYE
                     ANAALDFEREMAYGKHLDIPAGAAVRFEPGDKKEVQLVEYAGKRKIFGFRGMVNGPID
                     ESRVYRPTDENDEYAGVFGDNGAENVNKKGGKRS"
     gene            866..2581
                     /gene="ureC"
                     /locus_tag="ACGEXS_002188"
     CDS             866..2581
                     /gene="ureC"
                     /locus_tag="ACGEXS_002188"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HFF8013179.1"
                     /translation="MSFKMTQNQYTSLYGPTVGDSIRLGDTNLFAQIEKDYAVYGEEA
                     TFGGGKSIRDGMAQNPRVTRDDVNVADLVISNAVIIDYDKVVKADIGIKNGYIFAIGN
                     AGNPDIMDNVDIIIGSTTDIIAAEGKIVTAGGIDTHVHFINPEQAEVALESGITTHIG
                     GGTGASEGSKATTVTPGPWHIHRMLEAAEGLPINVGFTGKGQATNPTALIEQINAGAI
                     GLKVHEDWGATPSALSHALDVADEFDVQIALHADTLNEAGFMEDTMAAVKDRVLHMYH
                     TEGAGGGHAPDLIKSAAFSNILPSSTNPTLPYTHNTVDEHLDMVMITHHLNAAIPEDI
                     AFADSRIRKETIAAEDVLQDMGVFSMISSDSQAMGRVGEVITRTWQVAHRMKEQRGPL
                     DGDFEHNDNNRIKRYIAKYTINPAITHGISEYVGSIEPGKLADIVLWDPIFFGVKPEL
                     VVKGGLINSAVNGDANGSIPTSEPMKYRKMYGQYGGNLTSTSMTFVSKTAYENGINRA
                     LNLKRMVRPVKNIRQLSKADMKNNSATPKLDVDPQTYEVYVDGEKITSNAATELPLTQ
                     RYFLF"
     gene            2594..3046
                     /gene="ureE"
                     /locus_tag="ACGEXS_002189"
     CDS             2594..3046
                     /gene="ureE"
                     /locus_tag="ACGEXS_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HFF8013180.1"
                     /translation="MIVEEIQGNIANLSNSEKQKHVEKVYLENSDLVKRIQRVVTDHG
                     TEIGIRLKQPIDLQYGDILYADDHNMIIVDVNSEDLLVIQPRTLQEMGDIAHQLGNRH
                     LPAQFTETEMLVQYDYLVEDLLKSLGIPYVREDRKVNKAFRHIGHSHD"
     gene            3039..3728
                     /locus_tag="ACGEXS_002190"
     CDS             3039..3728
                     /locus_tag="ACGEXS_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HFF8013181.1"
                     /translation="MIDHTHLRLFQFCDSQFPTGAFSHSFGLETYIQRNIIHDDHTFI
                     AWLKMFLQEQLTYSDGLAMRLVYDALENDDTQKVLHIDKLMFVQNLPKETRVGAKQMG
                     TRMVKLALELYNSPWIAWYHQQMQDKKAKLNPAICFTMLGHHLGVDIETIIDYYLYQN
                     VSSLTQNAVRAIPLGQTAGQKIVTHMIPYIEGTRKQIFELKEADFGMTAPGLELNQMA
                     HENVNVRIFIS"
     gene            3741..4355
                     /gene="ureG"
                     /locus_tag="ACGEXS_002191"
     CDS             3741..4355
                     /gene="ureG"
                     /locus_tag="ACGEXS_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HFF8013182.1"
                     /translation="MANPIKIGIGGPVGAGKTQLIEKVVKRLSKEMSIGVITNDIYTK
                     EDEKILVNSGVLPESRIIGVETGGCPHTAIREDASMNFAAIDELLERHDDIELIFIES
                     GGDNLAATFSPELVDFSIYIIDVAQGEKIPRKGGQGMIKSDFFVINKTDLAPYVGASL
                     EQMAEDTKVFRGKRPFTFTNLKTDEGLDEVIDWIERDTLLKGLS"
     gene            4355..5191
                     /locus_tag="ACGEXS_002192"
     CDS             4355..5191
                     /locus_tag="ACGEXS_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HFF8013183.1"
                     /translation="MDEQQWTGQLDLTVFFDGNRSVSRDIFFEKALKVIRPVYLNQST
                     IPTFYIVNVGGGYLDGDRYRMNVNVEDNAKVTLTSQGATKIYKTPSNHVEQYQTFNLK
                     DNAYLEYVADPIIAYENAKFYQHNTFNLNNSSSLFYTDILTPGYSKTGEAFKYQYMHL
                     INEIYIEDELVTYDNLLLNPNKQSINEIGYMEHYSHYGSAYFIHEDVNQKLIDSVYET
                     ISSYSNTFDCRVAISQLPTHGFAVRIFAYRTQIIEKILGTIQSYIAENIYDRKLDFLR
                     KY"
     gene            complement(5380..5727)
                     /gene="sarR"
                     /locus_tag="ACGEXS_002193"
     CDS             complement(5380..5727)
                     /gene="sarR"
                     /locus_tag="ACGEXS_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarR"
                     /protein_id="HFF8013184.1"
                     /translation="MSKINDINDLVNATFQVKKFFRDTKKKFNLNYEEIYILNHILRS
                     ESNEISSKEIAKCSEFKPYYLTKALQKLKDLKLLSKKRSLQDERTVIVYVTDTQKANI
                     QKLISELEEYIKN"
BASE COUNT         1993 a          907 c         1071 g         1847 t
ORIGIN      
        1 attgtttgtt tcaatatcgg atatgctaaa aatataattt tctaattaca cattgaactt
       61 tgtatattac ttgtttatca caacaattag gattaatata aattttgtaa attgtatcta
      121 atatgaaatt gggggtttta atttgcattt tacacaacga gagcaagaca aattaatgat
      181 tgtagtggcg gcggaagttg cacgtcgtcg taaagcacgt ggtttgaaac taaatcatcc
      241 tgaggcatta gctttaatca gcgatgaatt attagaaggt gcacgcgatg gtaagaccgt
      301 tgcagagtta atgagttatg gtagacaaat tctaaacaaa gaagatgtca tggatggtgt
      361 cgaacacatg attacagata tcgaaatcga ggctacgttc cccgatggta ctaagttaat
      421 cacagtacat caccctattg tttaaaggag gcagtcaaat gataccagga gaaattatta
      481 caaaaagtac agaggttgaa attaataacc atcatcctga aacagttatc gaagttgaaa
      541 atacaggaga tcgaccaatt caagtgggct cacattttca tttttatgaa gcaaatgcag
      601 cattagattt cgaacgtgaa atggcatatg gaaaacattt agatattcca gctggagcag
      661 ctgttcgatt tgaacctggg gataaaaaag aagttcaatt agttgaatat gctggcaaac
      721 gtaaaatttt tggttttcgt ggtatggtca atggtcctat cgatgagtca cgtgtctatc
      781 gcccaactga tgaaaatgat gaatatgcag gtgtattcgg agataacggt gctgaaaacg
      841 tgaataaaaa aggaggaaaa agatcatgag ctttaaaatg acgcaaaatc aatatacgag
      901 cttatacggt ccaactgttg gagattccat tcgtttaggt gatacgaatc tatttgctca
      961 aatagaaaaa gactatgcgg tttatggtga agaagctact tttggtggtg gtaaatctat
     1021 tagagacggt atggcgcaaa atcctcgtgt aacacgtgat gacgtgaacg ttgcagacct
     1081 tgtcatttct aatgccgtta ttatcgatta cgataaagtg gttaaagctg atataggcat
     1141 taaaaatggt tatattttcg ccataggtaa tgccggcaac ccagatataa tggataatgt
     1201 cgacattatt ataggttcaa caacagatat cattgccgct gaaggtaaaa tcgtcactgc
     1261 tggtggtatt gatactcatg ttcattttat taatcctgaa caagcagagg tcgcattaga
     1321 aagtggtatt acgactcata ttggtggtgg tactggtgct tcagaaggtt ctaaagcaac
     1381 aactgtaact ccaggtccat ggcatattca tagaatgtta gaagctgccg aaggtttacc
     1441 gattaatgtc ggttttacag gtaaaggaca agcaacaaat ccaactgcac tcattgaaca
     1501 aatcaatgcc ggagcaattg gattaaaagt acatgaagac tggggtgcaa caccatctgc
     1561 tttgagtcat gcattagatg ttgctgatga atttgatgtt caaattgcat tacatgcaga
     1621 tactttaaat gaagcaggat ttatggaaga cacaatggct gctgttaaag accgtgtact
     1681 tcatatgtac catactgaag gtgctggtgg cggtcatgcg cctgatttaa ttaaatccgc
     1741 tgcattttca aatattttac cttcatctac aaatccaact ttgccttata cacataatac
     1801 tgtagatgaa catttagata tggtaatgat tactcaccat ttaaatgcgg ctattcctga
     1861 agatatcgca ttcgcagatt cacgtattcg taaagaaacg attgcagcag aagatgttct
     1921 gcaagatatg ggtgtattca gtatgattag ttccgattca caagcaatgg gccgtgtagg
     1981 tgaagtaatt acacgaacat ggcaagtagc acatcgcatg aaagaacaac gtggtccttt
     2041 agatggtgat tttgaacata atgataataa tcgcatcaaa cgttatatcg ctaaatatac
     2101 aattaaccca gcaattacac atggtatttc tgaatatgta ggatctatcg agccgggcaa
     2161 actagctgac attgtcttat gggacccaat tttctttggg gttaaacctg aattagttgt
     2221 aaagggcgga ttaattaact ctgccgtaaa tggcgatgca aatggttcta tacctacatc
     2281 tgaaccgatg aagtaccgta aaatgtatgg tcaatacggc ggaaacctta caagtacgtc
     2341 aatgacattc gtgtctaaaa ctgcttatga aaatggtatc aatcgtgcat taaatttaaa
     2401 acgcatggtg cgtccagtta aaaatattag acaattatct aaagcagata tgaaaaataa
     2461 cagtgcaaca cctaaattag acgttgatcc acaaacatat gaagtatatg tagatggaga
     2521 aaaaattaca agtaatgcag caactgagtt accattaact caaagatact tcttattcta
     2581 ggaggaacat agaatgattg ttgaagaaat ccaaggcaat attgccaatt tgtcaaattc
     2641 agaaaaacaa aagcacgtcg aaaaagtata ccttgaaaat tcagatcttg ttaaacgtat
     2701 tcaaagagtc gttacagacc atggcactga aataggcatt cgtttaaaac aacctattga
     2761 cttacaatat ggagatattt tatacgcaga tgaccataat atgattattg tggatgttaa
     2821 ttcagaagat cttttagtga ttcaaccaag aacattgcaa gaaatgggag atatagccca
     2881 tcaattagga aatcgtcatt tgccagcaca atttacagag actgaaatgc tagtacaata
     2941 tgattattta gttgaagatt tattaaaaag tttaggcatc ccatatgttc gagaagaccg
     3001 taaagttaat aaggctttca gacatatagg gcattctcat gattgatcat acacacttaa
     3061 gattatttca gttctgtgat tcacagtttc caacaggtgc tttcagtcat tcatttggtc
     3121 ttgaaacata tattcaacga aatataattc atgacgatca tacttttatt gcttggttaa
     3181 aaatgttttt acaagagcaa cttacctatt ctgatggttt agccatgcgt ttagtttatg
     3241 atgcattgga aaatgatgat acacaaaaag tattacacat agataaactg atgtttgttc
     3301 aaaacttacc taaagaaaca cgtgttggtg caaaacaaat ggggactcgc atggttaaat
     3361 tggctttaga actttataat agcccatgga ttgcttggta tcatcaacaa atgcaagata
     3421 agaaagcaaa gttaaatcca gctatttgtt ttactatgct aggccatcat ttaggtgtag
     3481 atattgaaac gattattgat tactatttat atcaaaatgt ttcaagttta actcaaaatg
     3541 ctgttcgtgc tattccactt ggacaaactg ctggtcaaaa gatagttact cacatgattc
     3601 cttatataga aggaacaaga aagcagattt tcgaattaaa agaagcagat tttggcatga
     3661 cagcgcctgg tttagaacta aatcaaatgg cgcatgagaa cgtcaatgtt agaattttca
     3721 tatcatagga ggttataatt gtggcaaatc cgattaaaat tggtattggt ggtcctgtag
     3781 gtgcaggtaa aacacaatta attgaaaaag ttgtaaaacg tctttcaaaa gaaatgagta
     3841 tcggcgttat tacaaatgat atatatacaa aagaagacga aaagatatta gtaaattcag
     3901 gagttctacc tgaaagtcgt atcattggtg ttgaaactgg tggatgtcct catactgcga
     3961 ttcgtgaaga tgcatctatg aactttgcag caatagacga attattagaa cgtcatgacg
     4021 atatagaact aattttcata gaatctggtg gcgataactt agcagcaaca tttagtccag
     4081 aacttgttga cttttcaata tatattatcg atgttgctca aggtgaaaag attccacgta
     4141 aaggtggtca aggtatgatt aagtcagatt tctttgtaat taacaaaact gatttagctc
     4201 cctatgtagg tgcatcatta gaacaaatgg ctgaagatac taaagtattt cgtggtaaac
     4261 gtccatttac ttttactaac ttaaaaaccg acgaaggttt ggatgaagtt atcgattgga
     4321 ttgaacgcga cactttactc aaaggattat cataatggat gaacaacaat ggactgggca
     4381 acttgattta acagtgtttt tcgatggcaa tcgatcagta tcaagagata ttttctttga
     4441 aaaagcactt aaagtgatac gtccagttta tctaaatcaa tctaccattc ctacatttta
     4501 tatagtaaat gtaggtggtg gctatttaga tggagatcgt taccgtatga atgtgaatgt
     4561 cgaagataac gctaaagtga cattgacatc tcaaggtgca acaaaaatat acaagacacc
     4621 ttccaatcat gttgagcagt atcaaacttt taatttgaaa gataacgcat atttagaata
     4681 tgtcgctgat ccaatcatcg catatgaaaa tgctaaattt tatcaacaca atacgttcaa
     4741 tctcaataat tctagttcat tattttatac tgatatttta actcctggtt attcaaaaac
     4801 tggcgaagcc ttcaagtatc aatatatgca tttaataaat gaaatttata ttgaagatga
     4861 gttagtcaca tatgataatt tattattgaa tcctaataaa caatcgatca atgaaatagg
     4921 ttatatggaa cattactctc attatggctc tgcttacttc atacacgaag atgttaacca
     4981 aaaactaatc gattcagttt atgaaaccat tagttcttat agcaatacat tcgattgtcg
     5041 tgttgctatt tctcaattgc ctacacatgg ttttgcagtt agaatttttg cttatcgcac
     5101 acaaattata gagaaaatac ttggaaccat ccaaagctat atcgcagaaa atatttatga
     5161 tcgtaaactt gattttctaa gaaaatatta aaataaaaag actagtgtac cttgtttcaa
     5221 gcttaataag ctgaaataag gtatggctag tctttaactt taattaaagc atcttcaata
     5281 tttaaatttt aagttaattt tatgtcccaa tgctgaataa ataacttcgt tttaataaat
     5341 tgttatcgtt caatgttatt aaacgcaatt aaccttgatt taatttttaa tgtattcttc
     5401 taattctgaa atcagttttt gaatatttgc tttttgtgta tctgtaacat aaacaataac
     5461 tgttctttcg tcttgtaaac ttcttttctt tgataacaat tttaaatctt ttagcttttg
     5521 taaagcttta gttaaatagt aaggtttgaa ctctgagcac ttagcaatct ctttagatga
     5581 gatttcgtta gactcacttc ttaaaatatg atttaaaata taaatttctt catagttcaa
     5641 attgaacttc ttttttgtat ctctgaaaaa cttcttaact tgaaatgttg cgttgactaa
     5701 atcattaatg tcattaattt tactcattat taaaccactc ctctgatgca catcttgttg
     5761 tagcaaggtt cacatgtaat ttattaaatc gaaactctat ccaactatgt tataaagt
//
LOCUS       DAXWFK010000152         5804 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.153, whole genome shotgun sequence.
ACCESSION   DAXWFK010000152 DAXWFK010000000
VERSION     DAXWFK010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5804
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.153"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(751..1194)
                     /locus_tag="ACGEXS_002194"
     CDS             complement(751..1194)
                     /locus_tag="ACGEXS_002194"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8013185.1"
                     /translation="MSIITRLFNNSDFEKLNQLCKLYDDLGYPTNENDLKKRLKKITN
                     HDDYFLLLLIKENKIIGLSGMCKMMFYEKNAEYMRILAFVIHSEFRKKGYGKRLLADS
                     EEFSKRLNCKAITLNSGNRNERLSAHKLYSDNGYVSNTSGFTKQL"
     gene            complement(1300..1737)
                     /locus_tag="ACGEXS_002195"
     CDS             complement(1300..1737)
                     /locus_tag="ACGEXS_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013186.1"
                     /translation="MFISLAIWSINSMGNKQCISLNLLLSDDTFAVISVMTTLGTAIL
                     GGIAPSIYGIFTSPIDKKSSFRAISDWEQEQRNNVKDLMMLLPFTLLSILIVIFIFQF
                     AYSKLFNTIAIIIFIIILVVMFFLILLICYKITVKICRYFSKE"
     gene            complement(2055..2645)
                     /locus_tag="ACGEXS_002196"
     CDS             complement(2055..2645)
                     /locus_tag="ACGEXS_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFF8013187.1"
                     /translation="MSELTAKQARFVNEYIRTLNVTQSAIKAGYNANSAHVTACRLLK
                     KPHIKQYIQEQKDKIIDENVLTAKELLHVLTNAAVGDETETKEVVVKRGEYKENPQSG
                     KVQLVYNEHVELIEVPIKPSDRLKARDMLGKYHKLFTDKHDINGNVPIFINIGEWDGG
                     DEGLDKAVKDVSNDNPNHTVIVDDIPLEDYESIRFL"
     gene            complement(2642..2854)
                     /locus_tag="ACGEXS_002197"
     CDS             complement(2642..2854)
                     /locus_tag="ACGEXS_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island family protein"
                     /protein_id="HFF8013188.1"
                     /translation="MTDDNVNDHIIKNHIEMIVDRLATDKEFYIFDSLIQGLSYQDIS
                     SALDCSEQSVILWYETILDKIVGVIK"
     gene            2915..3461
                     /locus_tag="ACGEXS_002198"
                     /pseudo
     CDS             2915..3461
                     /locus_tag="ACGEXS_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002476300.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(3556..5172)
                     /gene="groL"
                     /locus_tag="ACGEXS_002199"
     CDS             complement(3556..5172)
                     /gene="groL"
                     /locus_tag="ACGEXS_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500736.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFF8013189.1"
                     /translation="MVKQLKFSEDARQAMLRGVDQLANAVKVTIGPKGRNVVLDKEFT
                     APLITNDGVTIAKEIELEDPYENMGAKLVQEVANKTNEIAGDGTTTATVLAQAMIQEG
                     LKNVTSGANPVGLRQGIDKAVKVAVEALHENSQKVENKNEIAQVGAISAADEEIGRYI
                     SEAMEKVGNDGVITIEESNGLNTELEVVEGMQFDRGYQSPYMVTDSDKMVAELERPYI
                     LVTDKKISSFQDILPLLEQVVQSNRPILIVADEVEGDALTNIVLNRMRGTFTAVAVKA
                     PGFGDRRKAMLEDLAILTGAQVITDDLGLDLKDASIDMLGTASKVEVTKDNTTVVDGD
                     GDENSIDARVSQLKSQIEETESDFDREKLQERLAKLAGGVAVIKVGAASETELKERKL
                     RIEDALNSTRAAVEEGIVAGGGTALVNVYQKVSEIEAEGDIETGVNIVLKALTAPVRQ
                     IAENAGLEGSVIVERLKNAEPGVGFNAATNEWVNMLEEGIVDPTKVTRSALQHAASVA
                     AMFLTTEAVVASIPEKNNDQPNMGGMPGMM"
     gene            complement(5248..5532)
                     /gene="groES"
                     /locus_tag="ACGEXS_002200"
     CDS             complement(5248..5532)
                     /gene="groES"
                     /locus_tag="ACGEXS_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFF8013190.1"
                     /translation="MLKPIGNRVIIEKKEQEQTTKSGIVLTDSAKEKSNEGVIVAVGT
                     GRLLNDGTRVTPEVKEGDRVVFQQYAGTEVKRDNETYLVLNEEDILAVIE"
     gene            5707..>5804
                     /locus_tag="ACGEXS_002201"
     CDS             5707..>5804
                     /locus_tag="ACGEXS_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
                     /protein_id="HFF8013191.1"
                     /translation="MTRLWASLLTVIIYILSQFLPLLIVKKLPFVQ"
BASE COUNT         1880 a         1149 c          700 g         2075 t
ORIGIN      
        1 atgtcttatg tctttaatta agtaaaatgc acaagtacac ctttaaaaag atttactcaa
       61 aactaaacta tacctagtta accgcttgtg attaagaaat tttaaatgtg gtttctttta
      121 attatgtcaa ccacccataa ggattttaat caagcactca ttcattcctg cactcgcaat
      181 atgcattact tatagaagtg gatgcactct catattatgg caataccata actcccgcac
      241 cataatgatt taaataccat tatgaatctc aattacaaat tagcacataa aaaagacgcc
      301 tcccataagt ctagtcacca aatggaaaac gtctcgtaat taaaatatat ttgcgtgcat
      361 tctaatttgt acttagaata catcatactc gttcataatg taattttcta attagtcaaa
      421 actatataca gttttacatc attcccggca ttatgcacta ataaaaactt actttaatac
      481 acgctttaaa gattattaaa tcagcgtttt aatttttaga caacgctatt atttttagtt
      541 tttcaattct attatgtcat aattatgtca ctcaaaaact gttttccaac attgatttat
      601 tgttggaaaa tctcgaaaat ctagtctatt tctccaacaa tcgaagaatt atgccctttt
      661 ttctcccttt aaataagtca taatacgagg catacatgca acatttacaa taaaatatgt
      721 tcctacatcg tattatacga atgctcacac ttatagttgt ttagtaaacc cagatgtatt
      781 gctaacatac ccattatcac tatatagttt atgtgcagat agtctttcat ttctattacc
      841 actatttagt gttattgctt tacaattcaa ccgtttagaa aattcttcag aatcagctaa
      901 taatctcttt ccataacctt ttttcctaaa ttcagaatgt ataacaaacg caaggattct
      961 catatactct gcattttttt cgtaaaacat cattttacac ataccactta aaccaattat
     1021 tttattttct tttatcaaaa gcagtaggaa gtaatcatca tgattcgtta ttttctttag
     1081 tctctttttt aaatcattct catttgttgg ataacctaga tcatcatata atttacatag
     1141 ttgatttaat ttttcaaaat cactgttatt aaacaatctt gtaattatgc tcatataacc
     1201 caccctaaaa atattatgta cctattgtta catacaatag atacgcaaaa tcatttctca
     1261 ctgcaaatca ggacgtttct cagcgtaaac aaatcatttc tattcttttg aaaaatatct
     1321 gcatatcttc acagtaattt tataacagat taataaaatt aaaaaaaaca taacaacaag
     1381 tattataata aatattataa tagctatcgt attaaatagt ttagaatatg caaactgaaa
     1441 aataaaaatc acaattaaaa tactaagtaa tgtaaatggt aatagcatca tcagatcttt
     1501 aacattattt ctttgttctt gctcccaatc gctaatagcc ctaaaactac tttttttatc
     1561 tataggcgat gtaaaaatac catatattga aggtgctata cctcctaaaa tagcagttcc
     1621 caaagttgtc attactgaaa ttactgcgaa agtatcatcc gaaagcaata aattcaaact
     1681 aatgcattgt ttattaccca tcgaatttat tgaccaaata gctagagaaa taaacaaccc
     1741 aaaatttaaa ataaatgata tagtaatagc aattgtttac aaaacacgaa atttttcatt
     1801 tttatttata ttatccattt ttctcccttt ttcttaaatc attttattat atattacaat
     1861 aatcaatctg aaatgttgat gtaatttgaa aaaaatatca tacttttact cctgaaaacc
     1921 tccctaaatc atcaatatga aaatcagtat taggatattg cgtttacaac tcttttaaat
     1981 ctcactcatt cttctcatca tcttaaccga tactatcaat aaatactggc aacaccataa
     2041 aatatatacc caatttataa aaacctaatt gactcataat cttctaacgg tatatcatcc
     2101 acaatcacag tatgattagg attatcgtta gatacatctt tcactgcctt atctaacccc
     2161 tcatcacctc cgtcccattc accaatatta atgaatatag gtacattacc gtttatatca
     2221 tgcttatctg taaataattt atgatattta cccaacatat cacgagcttt taaacgatca
     2281 ctaggtttta ttggtacctc tatcagttca acatgttcat tatagactaa ttgtactttg
     2341 ccactttgtg gattttcttt atattctcca cgcttgacta caacttcttt cgtttctgtt
     2401 tcatcaccga ctgccgcatt cgttagcaca tgtaataact cttttgcggt taatacattc
     2461 tcatctataa tcttatcttt ttgttcttgt atatattgct tgatgtgcgg ctttttcaat
     2521 aacctacacg ctgtcacatg tgcgctattt gcgttatatc ctgcttttat ggcactttgt
     2581 gttacattca gtgttctaat atactcattc acaaaacgtg cttgctttgc agttaactca
     2641 ctcattttat cacccccaca attttatcta atatggtttc ataccataat attacagatt
     2701 gttctgaaca atctaaggca ctactaatat cttgataact aagtccttgt ataagggagt
     2761 caaaaatata aaactcttta tcggtcgcta atctgtcaac aatcatttct atgtgattct
     2821 ttataatatg atcattgaca ttatcgtctg tcatcaattc gtcagaatct tcatcaccta
     2881 ttaaaaagaa atcatcagta tttatttcat catcgccccg ttaactagct ttgaagtctt
     2941 tagcacactt gcatataccg gctgtcgtgc tggcgagata ctagcattga agtggtctga
     3001 tattgatttt gaaaacaaca cgattagtat tactaaaaca tattacaatc caaataataa
     3061 caagaagaaa tatcagatac ttccccctaa aactgaaagt tctatcggta agatttccgt
     3121 tgacccaaat gtaataaagg tgttgcgtga ttataagata aacgttcaaa ataattggaa
     3181 aaacgaatta tataatgata accattgtaa tgaaaaaact atcaatgtgg atacaaacca
     3241 ttatgtcacg taccagcata ctcaaaaata tctctacgca ctcattcaga tacacacatt
     3301 gcgcattgct catagaatca ggtgtacaca ttaaagaaat acaagaacga ttacggcata
     3361 aagatatcaa taccactatg aatatctatg ctaaaatcac aaattcatac aaaaaagacg
     3421 cctcccataa gtttagtcac caaatggaag acgtctcgta attaaaatat atttgcgtgc
     3481 attctaattt atacttagaa tgaatcatac tcgtgcataa tgtaattttc tagttagtca
     3541 aaactataaa cagttttaca tcattcctgg catgccaccc atgttaggtt ggtcattatt
     3601 tttttctgga attgatgcta caaccgcttc agtcgttaag aacattgctg caacacttgc
     3661 agcatgttgt aatgctgagc gtgttacttt agttggatca acgatacctt cttctaacat
     3721 attaacccac tcgtttgtag cagcgttaaa accaacaccc ggctctgcgt ttttcaaacg
     3781 ttctacaata acagaacctt ctaatcctgc attttcagca atttgacgaa ctggtgcagt
     3841 taatgcttta agtacaatat ttacacctgt ttcaatgtca ccttcagctt caatttcact
     3901 tactttttgg taaacattta ctaatgcagt accaccacct gcaacaatac cttcttcaac
     3961 tgctgcacgt gtagaattta atgcatcttc aatacgtaat ttacgttctt taagctctgt
     4021 ttcacttgct gcacctactt tgataactgc aacaccacct gctaatttag ctaagcgctc
     4081 ttgtaatttt tcacgatcaa agtcagattc agtttcttca atttgagatt tcaattggct
     4141 aacacgtgca tcaatgctgt tttcgtcacc gtcaccatca acaacagtgg tattatcttt
     4201 agttacttct actttacttg cagtacctaa catatcaatt gatgcatctt ttaaatctaa
     4261 gcctaaatca tcagtaatca cttgcgcacc agttaaaata gctaaatctt caagcatcgc
     4321 ttttctacga tcaccaaaac caggtgcttt tactgcaaca gctgtaaatg tgccacgcat
     4381 acggtttagc acgatatttg ttaatgcatc gccttcaact tcatcagcta caattaagat
     4441 tggacgatta gattgaacca cttgttctaa taaaggtaag atatcttgga aagacgagat
     4501 tttcttatct gttactaaaa tgtatgggcg ttctaattca gcaaccattt tatctgaatc
     4561 agtaaccata tacggtgatt gataaccacg atcaaattgc ataccttcaa ccacttctag
     4621 ttcagtgttt agtccatttg attcttcaat tgtaatgaca ccatcgttac ctactttttc
     4681 catagcttca gaaatataac gtccaatttc ttcatctgct gctgaaatcg cacctacttg
     4741 cgcaatttca tttttatttt caactttttg agaattttca tgtaacgctt caacagcaac
     4801 tttaactgct ttgtcgatac cttgtcgtaa accaactggg ttcgcaccac ttgtaacatt
     4861 tttcaagcct tcttgaatca ttgcttgagc taatactgtt gcagttgtcg taccgtcacc
     4921 agcaatttca tttgtcttat ttgcgacttc ttgaactagt ttagccccca tattttcata
     4981 tggatcttct aattcgattt ctttagcaat cgtcacacca tcattcgtaa ttaaaggtgc
     5041 tgtaaactct ttatctaata caacattacg tcctttagga ccaatcgtta ctttaactgc
     5101 atttgcaagt tggtcaacac cacgtaacat tgcttgacgt gcatcttcag agaatttcaa
     5161 ttgtttaacc ataaatgata aacctccatt tgtttagttg tgtttcattt tcgttctatt
     5221 taatttattt atgaattaag ttctgtatta ttcaataact gctaaaatat cttcttcatt
     5281 taataccaga tatgtttcat tatctcgttt aacttctgta ccagcatatt gttggaacac
     5341 gacacggtcc ccttctttca cttcaggagt cactcttgta ccatcattta ataggcgtcc
     5401 agttcctact gcaacgataa cgccttcgtt tgatttttct ttagcactat cagttaaaac
     5461 aataccactt ttagttgttt gttcttgttc ttttttctca ataatcacac gatttccaat
     5521 tggttttagc atgattgttc ctccttaaaa aacctaaagt ttagcactta acattaaaga
     5581 gtgctaacat acatttataa taatcaaatt tggtcaaaat ttcaagtcat aaccttttaa
     5641 ttaagttttg taacttcaag ttatttacga taaaataaat tataaacaaa tatttggagg
     5701 aaaattatga caagattatg ggcatcattg ctaactgtta ttatttatat attgtctcaa
     5761 tttttaccgc ttctcattgt aaaaaaatta ccatttgtac aata
//
LOCUS       DAXWFK010000153         5799 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.154, whole genome shotgun sequence.
ACCESSION   DAXWFK010000153 DAXWFK010000000
VERSION     DAXWFK010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5799
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.154"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..327)
                     /locus_tag="ACGEXS_002202"
     CDS             complement(<1..327)
                     /locus_tag="ACGEXS_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFF8013192.1"
                     /translation="MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVI
                     SITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDILDDFKGVDRNDIEIRQHRIILH
                     KGEEMIE"
     gene            complement(245..544)
                     /locus_tag="ACGEXS_002203"
     CDS             complement(245..544)
                     /locus_tag="ACGEXS_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGE"
                     /protein_id="HFF8013193.1"
                     /translation="MKSYKCKGSFLIDSMAGFLLIGLITLLLIPMMNQMQASINHKLQ
                     TIDASKVILTTVSKINKEELKKGVTIGKYDIKQSDQQICAISKNTTSYQKTCIQY"
     gene            complement(531..977)
                     /gene="comGD"
                     /locus_tag="ACGEXS_002204"
     CDS             complement(531..977)
                     /gene="comGD"
                     /locus_tag="ACGEXS_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFF8013194.1"
                     /translation="MEKQLQIRKQSAFTMIEMLVVMMLISIFLLLTMTSKGLSNLRVI
                     DDEANIISFITELNYIKSQAIANQGYINVRFYENSDTIKVIENNKIRFLKLKVGKIIN
                     VAKVDIIAFDKKGNINKFGSITIYNNNSIYRIIFHIEKGRIRYEKL"
     gene            complement(955..1266)
                     /gene="comGC"
                     /locus_tag="ACGEXS_002205"
     CDS             complement(955..1266)
                     /gene="comGC"
                     /locus_tag="ACGEXS_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFF8013195.1"
                     /translation="MFKFLKKTQAFTLIEMLLVLLIISLLLILIIPNIAKQTAHIQST
                     GCNAQVKMVNSQIEAYALKHNRNPSSIEDLIADGFIKEAQKTCKSGETITISNGEAVA
                     N"
     gene            complement(1280..2350)
                     /gene="comGB"
                     /locus_tag="ACGEXS_002206"
     CDS             complement(1280..2350)
                     /gene="comGB"
                     /locus_tag="ACGEXS_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFF8013196.1"
                     /translation="MKLQCINTFKLHSKKRQLSKAQQIDLLSNLCNLLKYGFTLYQSF
                     QFLNLQMTYKNKQLGTTILSEISNGAPCNQILSLIGYSDTIVMQVYLAERFGNIIDVL
                     EETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
                     SFQKTLSFFITSLPTIIVVMLIIVSMLAIIMKLIYNNLNMLNKINFVMKLPLISGYFQ
                     LFKTYFVTNELVLFYKNGITLQSIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQIL
                     EKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIKQTQFLQPILFLILG
                     LFIVAIYLVIMLPMFQMMQSIK"
     gene            complement(2322..3296)
                     /gene="comGA"
                     /locus_tag="ACGEXS_002207"
     CDS             complement(2322..3296)
                     /gene="comGA"
                     /locus_tag="ACGEXS_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFF8013197.1"
                     /translation="MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQ
                     IGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYLFNKIYFLRISTLPLSLGQESCVI
                     RIVPQFFQPQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
                     VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVI
                     QASLSGHLVLTTLHATDCKGAILRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLV
                     CEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTMHKYI"
     gene            complement(3348..3971)
                     /locus_tag="ACGEXS_002208"
     CDS             complement(3348..3971)
                     /locus_tag="ACGEXS_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFF8013198.1"
                     /translation="MRISSLTLGLVDTNTYFIENDKAVILIDPSGESEKIIKKLNQIN
                     KPLKAILLTHAHFDHIGAVDDIVDRFDVPVYMHEAEFDFLKDPVKNGADKFKQYGLPI
                     ITSKVTPEKLNEGSTEIEGFKFNVLHTPGHSPGSLTYVFDEFAVVGDTLFNNGIGRTD
                     LYKGDYETLVDSIQDKIFELEGDLPLFPGHGPYTTVDDEQLNPFLHG"
     gene            complement(3968..4297)
                     /locus_tag="ACGEXS_002209"
     CDS             complement(3968..4297)
                     /locus_tag="ACGEXS_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HFF8013199.1"
                     /translation="MAIVDVVVIPVGTEGPSVSKYIADIQKKLQEYKAMGKIDFQLTP
                     MNTLIEGELSDVLEVVQVIHELPFDKGLSRVCTNIRIDDRRDKSRKMNDKLTSVQKHL
                     ENSGENL"
     gene            complement(4297..5283)
                     /locus_tag="ACGEXS_002210"
     CDS             complement(4297..5283)
                     /locus_tag="ACGEXS_002210"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFF8013200.1"
                     /translation="MTKIILAADVGGTTCKLGIFTPELEQLHKWSIHTDTSDSTGYTL
                     LKGIYDSFVEKVNENNYNFSNVLGVGIGVPGPVDFEKGTVNGAVNLYWPEKVNVREIF
                     EQFVDCPVYVDNDANIAALGEKHKGAGEGADDVVAITLGTGLGGGIISNGEIVHGHNG
                     SGAEIGHFRADFDQRFKCNCGRSGCIETVASATGVVNLVNFYYPKLTFRSSILELIKE
                     NKVTAKAVFDAAKAGDQFCIFITEKVANYIGYLCSIISVTSNPKYIVLGGGMSTAGPI
                     LIENIKTEYHNLTFAPAQFETEIVQAKLGNDAGITGAAGLIKTYVLDKEGVK"
     gene            complement(5280..5483)
                     /locus_tag="ACGEXS_002211"
     CDS             complement(5280..5483)
                     /locus_tag="ACGEXS_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFF8013201.1"
                     /translation="MSRKINNFYDVQQLLKSYGFLIYFKNPEDMYEMIQQEISSLYQY
                     ELLSKEEYLKCTLIINQRRNEQK"
     gene            complement(5464..>5799)
                     /locus_tag="ACGEXS_002212"
     CDS             complement(5464..>5799)
                     /locus_tag="ACGEXS_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001652331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFF8013202.1"
                     /translation="DMTSGNYDNAQNIVKQTINKNYADDQTYYLSGMIMATINSKSEG
                     MTEWERGLRMFPKSGLLNFELAIANRSLNDDEKALKYVRKALNADPKNTDYINLEKEL
                     TKSNESKNK"
BASE COUNT         1878 a         1014 c          746 g         2161 t
ORIGIN      
        1 ttcgatcatt tcttcacctt tatgtaaaat gatacggtgt tgcctaattt caatatcgtt
       61 tctatctact cctttaaaat catctagaat atctcttgag aaaaattcga aatctacagt
      121 tgttaaatcc ctactttcaa ttagaaaagt tttactaagt ctaattaagt ctggaacaat
      181 tagtaaagtt atacttataa ccatcatcgc tactaacatt tcaatgagcg aaaaagcttt
      241 gacattaata ctgtatacat gtcttttgat aagaagtggt attttttgaa atagcacaaa
      301 tttgttggtc actttgctta atatcatact tccctatagt tacccccttc ttaagttctt
      361 ctttattaat tttagataca gtcgtcaaaa ttactttaga agcatcaatt gtttgtagtt
      421 tatggtttat actcgcttgc atttgattca tcattggtat caatagtaat gtaatcaatc
      481 caattagcaa aaatccagcc atactatcta ttaagaatga acctttacac ttatagcttt
      541 tcataacgaa ttcttccttt ttcaatatgg aatattattc tataaattga attgttattg
      601 taaattgtta tgctaccaaa tttattgata ttcccttttt tatcaaaggc aataatatca
      661 acttttgcaa cattaattat tttgcctact tttaatttta gaaatcgtat tttattattc
      721 tctattactt taatagtgtc actgttttca taaaatctaa cattgatata tccttgattt
      781 gctatagctt gcgacttaat ataattcaat tcagtaataa aagaaatgat atttgcctca
      841 tcatctatta ctctaagatt gcttaatcct ttagatgtca ttgtcaaaag tagaaatata
      901 ctgattaaca tcattaccac aagcatctca atcatagtaa atgctgactg ctttctaatt
      961 tgcaactgct tctccattac taattgtgat tgtctctcct gatttacatg tcttttgtgc
     1021 ttcttttata aaaccatctg caattaagtc ttcaatagac gatggatttc tattatgttt
     1081 caatgcatac gcttcaattt gactattaac catttttacc tgtgcattac aacctgttga
     1141 ttgtatgtga gcagtttgtt tagcaatatt tggaatgatt aaaattaata ataaactgat
     1201 gattaataac actaatagca tctctatcaa tgtaaacgct tgagttttct taagaaattt
     1261 aaacatatat taacctccat tattttatac tttgcatcat ttgaaacatt ggtaacataa
     1321 ttactaaata aattgcgaca ataaataaac ctaaaatcaa aaataaaata ggctgtaaaa
     1381 actgagtctg ttttatcgct ttatcttcta tttgttttac taatatttgc gaatataact
     1441 ttagttctac ttctagcttc cctctctttt caccttgtag cacaaactta attaattgag
     1501 gcttaaagca ttttagtttt tctaaaattt gaggcaaacc atatcccatt tctgaataag
     1561 ttaataagta tttacctaga aactgtctaa atggatcact actatggtta atataaacgt
     1621 ctactattga ttgaagtgta ataccatttt tataaaacaa cactaattca ttagttacaa
     1681 aataagtttt aaataattgg aaatagcctg atattagcgg tagtttcatc acaaagttta
     1741 tcttattgag catatttaaa ttgttataaa ttaatttcat aataatagcc aacatagata
     1801 ctattatgag cattactaca attatagtag gtaagctggt aatgaaaaaa gacaatgttt
     1861 tttgaaaaga agatagttga atattcatag aagtatataa ttgttgaaac tgtggaatta
     1921 ctgtgaggtt taatataata atcatagcaa taaagataga aactagtatt aaggggtatt
     1981 gcagtgtctt taacaatcgt tgttctgact ttctattcac tttcatataa tttacggttt
     2041 cttctagaac gtctataata ttgccaaatc tttctgccaa atatacttgc atgacgatag
     2101 tatcgctata acctatcagt gataatattt gattgcatgg tgcaccattt gaaatttcac
     2161 ttagaatggt ggtacctaat tgcttatttt tatatgtcat ttgaagattt aaaaattgaa
     2221 aactttgata cagagtgaaa ccatatttca acaaattaca taaatttgaa agtaagtcga
     2281 tttgttgggc cttacttaat tgtcgcttct tagaatgtag tttaaatgta tttatgcatt
     2341 gtagtttcac aaatgacacc tgcttttgtc atatcatcaa gtttatcttc taagttctta
     2401 aatgatgatg gtaatgaatg attatgggaa aagaaatatc ggagttgttg ctgagataga
     2461 atttcacata ctaattgtcg ctgttgctta atagtagtta caagtcgttg gtttataatt
     2521 aagttagttg cctgtatcaa ttcttgtaca gaaatgccca tttctaatag ccttaaaata
     2581 gcacctttac aatcagttgc atgcaatgta gtcagaacaa ggtgaccact taaactagcc
     2641 tgtataacac acttggcaac atctttatct ctgatttcac ctattaaaat aacatcagga
     2701 tcacatctta aaatagcttt aaacgaattt acatagttta tgccagcttt atcattcaca
     2761 ttaatttgga cgataccagg aatttgcatc tctacaggat cctctataga aattacattt
     2821 aaattcaagg ctttattcgc gtatgagacc atttgataca ttaatgtact ctttcctgaa
     2881 ccagttggtc cactaaacaa tagtaatcct tgtttcttat tcatgaggtg tttaaaatca
     2941 ttgaatttat aagttgattt ctgtggttga aaaaattgag gtacaattct gataacacaa
     3001 ctttcttggc caagtgacaa tggtaaagtt gatattctca aaaaatatat tttattgaaa
     3061 aggtaactat atcgaccgct ctgtgcgact tgctgtgtag aaacatcaag cccagcttga
     3121 aacttcatat aaactaataa cttttgataa atgctattcc caatttgttc atactgctcc
     3181 aagttatcat taattctaaa tttaatactt acttcatttt taactggaat aaaatgtaca
     3241 tcactcgctt tcatttctat cgctttatta attatttctt gaaatagaat cttcaaaaaa
     3301 acacctccta catataatca cgtaggaggt gtttttatta cttcaattta accgtgtaaa
     3361 aatggattta attgttcatc atcaaccgtc gtatatggac catgtccagg gaataaaggt
     3421 aaatcgcctt ctaattcaaa tattttatct tgaatagaat caactagcgt ttcataatca
     3481 cctttatata aatctgtacg tccgattcca ttattaaata atgtatctcc aacaactgcg
     3541 aattcatcga acacatatgt taaacttcct ggtgaatgtc caggtgtgtg taacacatta
     3601 aacttaaatc cttctatttc tgtgctacct tcgtttaact tttcaggagt taccttactt
     3661 gtaataattg gtaatccata ttgcttaaat ttatctgccc catttttaac gggatctttt
     3721 agaaaatcaa actctgcttc atgcatataa accgggacat cgaatcgatc aactatatca
     3781 tcgactgctc cgatatgatc aaagtgtgca tgtgttaata aaatagcttt taacggttta
     3841 tttatttggt ttaatttttt aataattttt tcactttcac ctgaagggtc aatcagaata
     3901 acagctttgt cattttcgat gaaatacgta ttagtatcaa ctaagcctaa agttaagctt
     3961 gaaatcctca taggttttca ccactatttt ctaaatgttt ttgtactgat gttagtttat
     4021 cattcatttt tctagatttg tctcgtcggt catcaatacg gatatttgta caaactctac
     4081 ttaaaccttt atcaaaaggt aattcatgta tcacttgcac aacttctaat acatcgctta
     4141 attcaccttc aattagagta ttcattggtg ttaattgaaa atcaatttta cccatcgctt
     4201 tatattcttg aagttttttc tgaatatctg caatatattt actaacactc ggaccttccg
     4261 ttccaactgg aataacaacc acatcaacaa tagccattat tttaccccct ctttatctaa
     4321 tacataggtc ttgattaatc ctgctgctcc tgtaatacct gcatcattac ctaatttcgc
     4381 ttgtacaatt tcagtttcaa attgagcagg tgcaaatgtt aaattatgat attctgtttt
     4441 aatattttca attaaaatag gtcctgcagt agacattcct ccacctagaa cgatatattt
     4501 cggattactt gtaacactaa taatactaca taaatatcca atatagtttg caaccttttc
     4561 agtaatgaaa atacagaatt ggtcaccagc ttttgccgca tcaaaaacag cttttgctgt
     4621 aaccttattt tctttaatca attctaatat agaagatcta aacgtcaact tcggatagta
     4681 gaagttaact aagttaacaa cgcctgtcgc tgaagcaact gtttcaatac atccagaacg
     4741 accacaatta catttaaatc gttgatcgaa gtctgctcta aaatgaccta tttctgcgcc
     4801 agagccatta tgaccatgta cgatttcacc atttgaaata attcctccac ctagacctgt
     4861 accaagtgtg atggcaacaa catcatcggc accttcacca gcacctttgt gtttctcccc
     4921 taaagcagct atgttagcat cattatctac atacactgga caatcaacga attgttcaaa
     4981 aatctcacgt acattaactt tttctggcca atataagttt actgctccat ttactgtacc
     5041 tttttcaaag tcaacaggac ctggtacacc aatacctacg ccaagtacat ttgaaaaatt
     5101 ataattattt tcatttactt tttcaacaaa cgaatcataa attcctttca aaagtgtata
     5161 tcctgtacta tcagatgtat cagtgtgaat agaccattta tgtaattgtt ctaattcagg
     5221 tgtgaaaata cctaatttac aagtcgtccc gcctacatca gctgctaaaa taatttttgt
     5281 catttctgtt cattccttct ctgattaatt atcaacgtac atttcaaata ttcttcctta
     5341 gataacagtt catattgata caatgatgaa atctcctgtt gaatcatttc gtacatatct
     5401 tctggatttt taaaatatat tagaaatccg taacttttca ataactgttg tacatcataa
     5461 aagttattta tttttcgact catttgattt agtcaactct ttttctaagt taatataatc
     5521 tgtattttta gggtctgcat ttaatgcttt acgcacatat tttaatgctt tttcatcatc
     5581 atttaatgaa cgatttgcta tcgctaactc aaaatttaat aaacctgatt taggaaacat
     5641 tctaagtcct cgctcccatt ctgtcatacc ttcggactta gaattgatag tagccataat
     5701 cataccactt aaataatatg tttgatcatc ggcataattt ttatttattg tctgcttcac
     5761 aatattttga gcattatcat aattaccact agtcatatc
//
LOCUS       DAXWFK010000154         5771 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.155, whole genome shotgun sequence.
ACCESSION   DAXWFK010000154 DAXWFK010000000
VERSION     DAXWFK010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5771
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.155"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..306)
                     /locus_tag="ACGEXS_002213"
     CDS             complement(<1..306)
                     /locus_tag="ACGEXS_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013203.1"
                     /translation="MKQTFITLGEGLTDLFEFMTMIEYNHQRIDKIIYFHSPQAENKK
                     SSVAIIMNPTTGNHFQAFYIMINAIKYPYPDSNKKFQMINDCAEKFDIPILGIDVQPP
                     "
     gene            376..918
                     /gene="rsmD"
                     /locus_tag="ACGEXS_002214"
     CDS             376..918
                     /gene="rsmD"
                     /locus_tag="ACGEXS_002214"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFF8013204.1"
                     /translation="MRVIAGKHKSKALESMEGRNTRPTMDKVKEGIFNSLYDVSGIGL
                     DLFAGSGALGIEALSRGMDKVIFVDQNFKAVKVIKSNLANLDLEAQSEVYKNNADRAL
                     KALSKRDIQFDVIFLDPPYNKGLIDKALKLISEFNLLKENGIIVCEFSNHEEIDYQPF
                     NMIKRYHYGLTDTLLLEKGE"
     gene            920..1402
                     /gene="coaD"
                     /locus_tag="ACGEXS_002215"
     CDS             920..1402
                     /gene="coaD"
                     /locus_tag="ACGEXS_002215"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFF8013205.1"
                     /translation="MEHTIAVIPGSFDPITYGHLDIIERSTDRFDEIHVCVLKNSKKE
                     GTFSLEERMDLIEQSVKHLPNVKVHQFSGLLVDYCEQVGAKTIIRGLRAVSDFEYELR
                     LTSMNKKLNNEIETLYMMSSTNYSFISSSIVKEVAAYRADISEFVPPYVEKALKKKFK
                     "
     gene            complement(1464..2603)
                     /locus_tag="ACGEXS_002216"
     CDS             complement(1464..2603)
                     /locus_tag="ACGEXS_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFF8013206.1"
                     /translation="MKSVGLITEYNPFHNGHQYHINQSKKLTNADVTIAIMSGNFVMR
                     GEPAIYNKFTRAKMALSTADLVIELPATASLSSGDHFAELAVKVADYMSVDTIAFGSE
                     NNDIKTLKQLAHSINEIEQSESFSQKVKEGKSYPRIISELLEHHEALASPNNILGISY
                     LKAIAKNAKNINAISIKRENAQHHDSLIQHHQFASGTSIRTSIISQDDHWHHVVPKDI
                     QHLYVTPHITLNQIFPYLKYQIIAMTTDSLKNIYTVTEGFENRLKSNIYEATDFHHFV
                     KLLKTKRYTYTHIQRLLMNVLLNIKPTDVTSNIHAVKVLAMNDRGRQYLKHLKTAFPE
                     RQYITNINKSNAHYFTNEIKATHIYNAISGQQQTDFNTPVIQQYR"
     gene            2730..3287
                     /locus_tag="ACGEXS_002217"
     CDS             2730..3287
                     /locus_tag="ACGEXS_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFF8013207.1"
                     /translation="MKWSITQLRKYQGKPFEFDQTVSFDNLKESLDLIDLSPITIQGQ
                     LTIKSTEVVADIHITGTYTMPCARTLVPVKVPLDVTTTEVFDLEGYNQYNDDQDDVDE
                     HYHIIKDGMVNLQDIVEDIVIIEKPMRAYSEQSDQMLTVGNGWEVIDEDQLDELAKQQ
                     EQDDSESRQVDPRLQKLQQLYDKEQ"
     gene            3367..3540
                     /gene="rpmF"
                     /locus_tag="ACGEXS_002218"
     CDS             3367..3540
                     /gene="rpmF"
                     /locus_tag="ACGEXS_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFF8013208.1"
                     /translation="MAVPKRRTSKTRKNKRRTHFKISVPGMTECPNCGEYKLSHRVCK
                     NCGSYNGEEVAAK"
     gene            complement(3694..5631)
                     /gene="isdB"
                     /locus_tag="ACGEXS_002219"
     CDS             complement(3694..5631)
                     /gene="isdB"
                     /locus_tag="ACGEXS_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme uptake protein IsdB"
                     /protein_id="HFF8013209.1"
                     /translation="MNKQQKEFKSFYSIRKSSLGVASVAISTLLLLMSNGEAQAAAEE
                     TGGTNTEAQPKTEAVASPTTTSEKAPETKPVANAVSVSNKEVEAPTSETKEAKEVKEV
                     KAPKETKEVKPAAKATNNTYPILNQELREAIKNPAIKDKDHSAPNSRPIDFEMKKKDG
                     TQQFYHYASSVKPARVIFTDSKPEIELGLQSGQFWRKFEVYEGDKKLPIKLVSYDTVK
                     DYAYIRFSVSNGTKAVKIVSSTHFNNKEEKYDYTLMEFAQPIYNSADKFKTEEDYKAE
                     KLLAPYKKAKTLERQVYELNKIQDKLPEKLKAEYKKKLEDTKKALDEQVKSAITEFQN
                     VQPTNEKMTDLQDTKYVVYESVENNESMMDTFVKHPIKTGMLNGKKYMVMETTNDDYW
                     KDFMVEGQRVRTISKDAKNNTRTIIFPYVEGKTLYDAIVKVHVKTIDYDGQYHVRIVD
                     KEAFTKANTDKSNKKEQQDNSAKKEATPATPSKPTPSPVEKESQKQDSQKDDNKQLPS
                     VEKENDASSESGKDKTPATKPTKGEVESSSTTPTKVVSTTQNVAKPTTASSKTTKDVV
                     QTSAGSSEAKDSAPLQKANIKNTNDGHTQSQNNKNTQENKAKSLPQTGEESNKDMTLP
                     LMALLALSSIVAFVLPRKRKN"
BASE COUNT         1753 a          808 c         1015 g         2195 t
ORIGIN      
        1 agggggctgt acatcgatac ctaaaattgg tatgtcgaat ttttcagcac aatcatttat
       61 catttgaaac tttttatttg aatctggata tggatattta atagcgttta tcatgatata
      121 aaatgcttgg aaatgattgc cagttgtagg gttcataatg attgctacag atgacttttt
      181 attttcagct tgtggtgaat gaaaatagat aattttatca atacgttgat ggttatattc
      241 aatcatcgtc atgaattcga acaaatctgt tagaccttca ccaagtgtaa taaatgtttg
      301 tttcattggt ttatcattct cctttttctc aataaattat attaaataac taaatgaatt
      361 ggaagtgtga acaacatgcg cgtcattgca ggtaaacata aaagtaaagc tttagaaagt
      421 atggaaggcc gtaatacgag accaactatg gataaagtta aagaaggtat ctttaatagt
      481 ttatatgatg tgtcaggtat aggtttagat ttatttgcag gaagcggggc gcttggaata
      541 gaagcactct ctcgaggtat ggataaggta atctttgttg atcaaaattt taaagctgta
      601 aaagttatta aatcaaatct tgcgaatttg gatttagagg cacaatctga agtttataaa
      661 aataatgcag atagagcttt aaaagcattg tcaaaacgtg atattcaatt tgatgtcatt
      721 ttcttagatc caccttataa taaaggtctc attgataaag ctttaaaact aatttcagag
      781 tttaatttat tgaaagaaaa tggtatcatc gtttgtgaat ttagcaatca tgaagaaata
      841 gattatcaac cgtttaatat gattaaacgt taccattatg ggttgacaga cacattgtta
      901 ttagaaaagg gagaatagca tggaacatac aatagcggtc attccgggta gttttgaccc
      961 cattacttat ggtcatttag acattattga gagaagtaca gatagatttg atgaaattca
     1021 tgtctgtgtt cttaaaaata gtaaaaaaga aggtacgttt agtttagaag agcgtatgga
     1081 tttaattgaa caatctgtta aacatttacc taatgtcaag gttcatcaat ttagtggttt
     1141 actagtcgat tattgtgaac aagtaggagc taaaacaatc atacgtggtt taagagcagt
     1201 cagtgatttt gaatatgaat tacgcttaac ttcaatgaat aaaaagttga acaatgaaat
     1261 tgaaacgtta tatatgatgt ctagtactaa ttattcattt ataagttcaa gtattgttaa
     1321 agaagttgca gcttatcgag cagatatttc tgaattcgtt ccaccttatg ttgaaaaggc
     1381 attgaagaag aaatttaagt aataaaaata acagtatttt aggtttatca tggtttacaa
     1441 tcctaaaata ctgttttcat ttgttaacga tattgctgta tgacaggcgt gttgaaatct
     1501 gtttgttgtt gcccgcttat tgcattgtat atgtgtgttg ctttgatttc atttgtgaag
     1561 taatgtgcat tgcttttgtt aatattggtt atatattgtc tttctgggaa cgctgttttt
     1621 aaatgcttta aatattgtct gccacggtcg ttcatcgcta atactttaac tgcgtgaatg
     1681 ttactcgtaa catctgtagg tttaatgttt aataatacat tcattaacag tctttggata
     1741 tgcgtatatg tataacgctt tgtttttagt aattttacaa aatgatgaaa atcagttgct
     1801 tcataaatgt tagatttcaa acgattttca aaaccttcag taacagtata aatatttttt
     1861 aatgaatctg tagtcatagc tatgatttga tatttcaaat atggaaatat ttgatttaat
     1921 gttatatgag gtgttacgta caagtgttga atatctttag gtaccacatg atgccaatga
     1981 tcatcttgac taatgattga tgttctaata gatgtaccac ttgcaaactg atggtgttga
     2041 attaatgaat catgatgttg agcattttct cgtttgatag aaattgcatt gatgttttta
     2101 gcatttttag caattgcttt caggtaacta ataccaagta tgttgttagg acttgctagt
     2161 gcttcatgat gctctaataa ttcgctaatg atacgagggt agcttttacc ttcttttact
     2221 ttttgtgaaa aggattcaga ttgttcaatt tcattaatgc tgtgtgctaa ttgctttaat
     2281 gttttgatat cattattttc actaccaaat gcaattgtat cgacactcat ataatctgcg
     2341 actttaactg ctagttcggc aaaatgatcg ccagatgata aactggcagt tgctggtagt
     2401 tcgataacta aatcagctgt tgataatgcc atttttgcac gagtaaactt attatagatt
     2461 gctggttcgc cacgcatgac aaagttacca ctcattattg caatagtaac gtcagcattt
     2521 gtaagttttt tagattgatt aatatgatat tgatgcccat tatgaaaggg attatattct
     2581 gtgattaagc caacgctttt catttgcaat tcgtcctttc ggtattcatt attaatattg
     2641 taacagaaca tgatatgtta agaaaaaatc ttgacaactt gttcttagaa agttaaaata
     2701 aattttgtgc ttgttagagg tgaagccata tgaaatggtc aattacgcaa ttaaggaaat
     2761 atcaaggtaa gccatttgaa tttgatcaaa cggtgagttt tgacaattta aaagaatcat
     2821 tagatttaat tgatttatct ccaattacaa tccaaggtca gttaaccatt aagtcaacag
     2881 aagtcgttgc ggatattcac attactggaa cgtatacaat gccttgtgca cgtactcttg
     2941 taccagtaaa agtcccacta gatgtaacta ctacagaagt atttgattta gaagggtaca
     3001 atcagtataa cgatgatcaa gatgatgtag atgaacacta tcacattatt aaagatggta
     3061 tggttaatct tcaggatatt gtcgaggata tagttattat tgagaaacca atgagagctt
     3121 attcagagca aagtgaccaa atgttgacag taggtaatgg ttgggaagta atcgatgagg
     3181 atcaattaga tgagcttgct aaacagcaag aacaagatga ttcagaatca cgacaagttg
     3241 atccaaggct tcaaaaatta caacaattat atgataaaga gcaataagta gtttaattaa
     3301 tgatataatg ttgttaatta aactaacatt tagtattttt cgtttataat ctaaggagga
     3361 tatatcatgg cagtaccaaa aagaagaact tctaaaacta gaaaaaacaa acgtcgtacg
     3421 catttcaaaa tttcagtacc aggtatgact gaatgcccaa actgtggcga atacaaatta
     3481 tcacaccgtg tatgtaaaaa ctgtggttct tacaatggcg aagaagtagc agctaaataa
     3541 ttttagttac tcaatataaa aagtcccgct taaaatgatt gttttaagtg ggacttttta
     3601 tattgcgaaa aataattggc gaacgaggta actggatacc tcatccgcca attaaaattt
     3661 gttaatttaa taattaaata taaagacgat ttattagttt ttacgttttc taggtaatac
     3721 gaatgcaacg atgctactta aagctaataa tgccattaat ggtaatgtca tatctttatt
     3781 tgattcttca ccagtttgtg gtaatgattt tgctttattt tcttgtgtat ttttattgtt
     3841 ttggctttga gtgtgtccat catttgtgtt tttaatgttt gctttttgta atggagcact
     3901 atcttttgct tcgctagaac ctgctgaagt ttgaacaaca tcttttgttg tttttgatga
     3961 agcagttgtt ggttttgcaa cattttgagt cgtagatact accttagttg gagttgtact
     4021 acttgattct acttcacctt tagttggttt tgtagcaggc gttttgtctt tacctgactc
     4081 actagatgcg tcattttctt tttcaacact tggtaattgt ttattgtcat ctttttggct
     4141 gtcttgtttt tgtgattctt tttcaacagg tgatggtgtt ggtttgctag gcgtagctgg
     4201 agtagcttcc ttcttagctg agttatcttg ttgttctttt ttgttagatt tatcggtatt
     4261 ggcttttgta aatgcttctt tatcaacgat tctgacatgg tattgtccat cataatcaat
     4321 cgtttttacg tgaactttaa cgatagcatc atatagagtt ttaccttcaa catatgggaa
     4381 aataattgtt ctagtattat ttttagcatc tttgcttata gttctaacac gttgaccttc
     4441 aaccatgaaa tctttccagt aatcgtcatt agtagtttcc atgaccatat attttttgcc
     4501 gttaagcata cctgttttaa tagggtgttt aacaaaagta tccatcatag attcgttatt
     4561 ctcaacactt tcataaacaa catattttgt atcttgtaaa tcagtcattt tttcatttgt
     4621 tggttgtaca ttttggaatt cagtaatagc tgatttcact tgctcatcta aagctttctt
     4681 tgtatcctct aatttcttct tgtactcagc ctttaatttt tcaggaagtt tatcttgaat
     4741 tttatttaat tcataaactt gtctttctag tgttttcgct tttttatatg gcgctaataa
     4801 tttttcagct ttataatctt cttcagtttt gaatttatct gcactgttat aaattggttg
     4861 tgcgaattcc attaatgtgt aatcgtattt ttcttctttg ttattgaagt gtgttgaact
     4921 aacaatttta acagcttttg ttccgtttga tacagagaag cgaatgtaag cataatcttt
     4981 aacagtatcg tatgatacta atttaattgg caactttttg tcaccttcat aaacttcaaa
     5041 ttttctccaa aattgacctg attgtaatcc taattcaatt tctggttttg aatcagtgaa
     5101 aataactcta gcaggtttaa cagaacttgc ataatgataa aactgttgag ttccatcttt
     5161 ctttttcatt tcaaaatcaa ttggacgaga gtttggtgcg ctatgatctt tgtcttttat
     5221 tgcagggttt ttaatcgctt ctctaagttc ctgattcaaa ataggatatg tattgttagt
     5281 ggcttttgct gctggtttaa cttcttttgt ttccttaggg gctttaactt ctttaacttc
     5341 tttagcttct tttgtttcag aagtaggggc ctcaacttct ttattagata ctgagacagc
     5401 attagctact ggtttagttt ctggagcttt ttcagatgtt gttgttggac ttgcaactgc
     5461 ttcagttttt ggttgtgctt ctgtatttgt accacctgtt tcttcagctg ctgcttgtgc
     5521 ttcgccattt gacattaata ataaaagtgt actaattgct acagatgcaa cgcctagtga
     5581 tgactttcta attgaataaa atgatttaaa ttctttttgc tgtttgttca tgttgtagaa
     5641 acaactccta attgtatatt atcaactgat aatcattatc aattgattac ttgtattgta
     5701 cattgaaatg ttagttaatt gcaacgttat tttataaaaa aataacctat gttatagatt
     5761 atgaaatatc t
//
LOCUS       DAXWFK010000155         5590 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.156, whole genome shotgun sequence.
ACCESSION   DAXWFK010000155 DAXWFK010000000
VERSION     DAXWFK010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.156"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            97..1488
                     /locus_tag="ACGEXS_002220"
     CDS             97..1488
                     /locus_tag="ACGEXS_002220"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HFF8013210.1"
                     /translation="MAKDMDTIVSLAKHRGFVFPGSDIYGGLSNTWDYGPLGVELKNN
                     VKKAWWQKFITQSPFNVGIDAAILMNPKVWEASGHLNNFNDPMIDNKDSKIRYRADKL
                     IEDYMQDVKGDENFIADGLSFEQMKKIIDDEGIVCPVSKTANWTEIRQFNLMFKTFQG
                     VTEDSTNEIFLRPETAQGIFVNYKNVQRSMRKKLPFGIGQIGKSFRNEITPGNFIFRT
                     REFEQMELEFFCKPGEEIEWQNYWKTFASDWLTSLNMSSENMRLRDHDEDELSHYSNA
                     TTDIEYKFPFGWGELWGIASRTDFDLRKHAEHSGEDFRYHDPETNEKYIPYCIEPSLG
                     ADRVTLAFLCDAYDEEGVEGSKDARTVLHFHPALAPYKAAILPLSKKLSGEAIKIFEQ
                     LSSKFSIDFDESQSIGKRYRRQDEIGTPYCVTFDFDSLEDNQVTVRDRDSMEQVRMPI
                     SELEAFLTEKTKF"
     gene            complement(1638..2384)
                     /gene="recO"
                     /locus_tag="ACGEXS_002221"
     CDS             complement(1638..2384)
                     /gene="recO"
                     /locus_tag="ACGEXS_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFF8013211.1"
                     /translation="MRQKGIIIKAVDYGESDKIITILNEHGAKVPLMARRAKKVKTGL
                     QAQTQLFVYGLFIYNQWRGMGTLNSVDVISQHYKLQMDLYVSSYAALAAETIERSMDE
                     GDIAPYNYQLLQFVLEKIESGTSAQLMSVVVMLKCMKRFGFTASFNRCAVSGNDTQAD
                     LIGYSFKFDGAISRQEASKDVHAVILSNKTLYLLDVLQKLPIDKMNSLNIHQEIIDEM
                     SDIILMLYREYAGMFFKSQKLINQLKRLEQ"
     gene            complement(2412..3311)
                     /gene="era"
                     /locus_tag="ACGEXS_002222"
     CDS             complement(2412..3311)
                     /gene="era"
                     /locus_tag="ACGEXS_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFF8013212.1"
                     /translation="MTEHKSGFVSIIGRPNVGKSTFVNRVIGHKIAIMSDKAQTTRNK
                     IQGVMTRDDAQIIFIDTPGIHKPKHKLGDYMMKVAKNTLSEIDAIMFMVNANEEIGRG
                     DEYIIEMLKNVKTPVFLVLNKIDLVHPDELMPKIEEYQSYMDFTEIVPISALEGLNVD
                     HFIDVLKTYLPEGPKYYPDDQISDHPEQFVVGEIIREKILHLTSEEIPHAIGVNVDRM
                     VKESEDRVHIEATIYVERDSQKGIVIGKGGKKLKEVGKRARRDIEMLLGSKVYLELWV
                     KVQRDWRNKVNFIRQIGYVEDQD"
     gene            complement(3312..3716)
                     /gene="cdd"
                     /locus_tag="ACGEXS_002223"
     CDS             complement(3312..3716)
                     /gene="cdd"
                     /locus_tag="ACGEXS_002223"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFF8013213.1"
                     /translation="MSYQPHYFQEVRKAQQESYSPYSQFKVGAYLKTKDGRTFYGTNV
                     ENASYPLSICAERASLVSAISQGYRPGDFESITVTVDADKPSSPCGACRQVLKELCDD
                     DMPVYMTNHKGDMVMMTVAELLPFGFSGKDLE"
     gene            complement(3727..4071)
                     /locus_tag="ACGEXS_002224"
     CDS             complement(3727..4071)
                     /locus_tag="ACGEXS_002224"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFF8013214.1"
                     /translation="MKRFKYALDGLKILIQKDYKFLLHVFAMIVAIVFGLVLNINRIE
                     WIFILIAIALVLTVEALNTAIEYVVDLVTVEYHDLAKYAKDIAAFSVLIVSILAFIIG
                     LIVFLPHFIALF"
     gene            complement(4074..4541)
                     /gene="ybeY"
                     /locus_tag="ACGEXS_002225"
     CDS             complement(4074..4541)
                     /gene="ybeY"
                     /locus_tag="ACGEXS_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFF8013215.1"
                     /translation="MFTIDFSDHTGLVKDAWYKQIEDLLEFAKKEEHIEDDAELSVTF
                     VDKQEIQEINRTYRDKDKVTDVISFALEEDEPEIDFSGLDIPRVLGDIIICTDVAQEQ
                     ANNYGHSFERELGFLALHGFLHLLGYDHMTEADEKEMFGRQDTILNAYGLTRD"
     gene            complement(4542..5489)
                     /locus_tag="ACGEXS_002226"
     CDS             complement(4542..5489)
                     /locus_tag="ACGEXS_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFF8013216.1"
                     /translation="MPGIIQIDDMNQSQALIGNNDEHLKAIEESFDVVIHARGQEVAV
                     KGTKIENVEKAESVLINLLKVIDLGNNITIKDVEAAIKMAHNNTIQHLLDLYDEEITK
                     DAFGKTIRAKTMGQRIYVNAMKNNDLVFGIGPAGTGKTFLAVVYAAKQLRKGAVKRIV
                     LTRPAVEAGESLGFLPGDLKEKVDPYLRPLYDGLYTVLGREQTERFIERGIIEIAPLA
                     YMRGRTLEDAFVILDEAQNTTHAQMKMFLTRLGFGSKMVVTGDQTQIDLPKGVKSGLK
                     EAVSRLHNVKGISILKLDQSDVVRHPLVSKIIEHYEGEN"
BASE COUNT         1787 a         1047 c          815 g         1941 t
ORIGIN      
        1 tacaatttaa aatattaact cgtcccatgg aagtaaagca tatttactat gctgttattt
       61 tccatggggc gttttcatgt atgaggagag gtaattatgg caaaagatat ggatacaatt
      121 gtttcattag caaaacacag aggttttgtg ttccctggta gtgatattta cggtggttta
      181 tcaaacacat gggattatgg tcctttaggt gttgaattaa agaataatgt taaaaaagct
      241 tggtggcaaa aattcattac acaatcaccg tttaacgttg gtatcgatgc tgcaatctta
      301 atgaatccaa aagtatggga agcttcagga cacttaaaca acttcaacga cccaatgatt
      361 gataataaag atagtaaaat tcgatatcgc gctgataaat taattgaaga ttatatgcaa
      421 gatgttaaag gtgatgaaaa cttcattgcc gatggtttaa gttttgaaca aatgaaaaaa
      481 attattgacg atgaaggtat tgtttgtcct gtaagtaaaa ctgctaactg gactgaaatt
      541 cgccaattca atttaatgtt taaaacattc caaggtgtaa ctgaagattc tacaaatgaa
      601 attttcttac gtcctgaaac agcacaaggt atttttgtaa actataaaaa cgtgcaacgt
      661 tcaatgcgta aaaaattacc atttggtatc ggtcaaattg gtaaatcatt ccgtaatgaa
      721 atcactccag gtaacttcat tttcagaaca agagaatttg aacaaatgga acttgaattc
      781 ttctgtaaac ctggagaaga aatcgaatgg caaaattatt ggaaaacttt tgcaagtgac
      841 tggttaacaa gcttaaatat gagcagtgaa aatatgcgtt tacgtgatca tgatgaagat
      901 gaattatctc attactcaaa tgcaacaact gatattgaat ataaattccc atttggttgg
      961 ggtgagttat ggggtatcgc aagtcgtaca gacttcgact tacgtaaaca tgctgaacac
     1021 tctggtgaag atttcagata ccatgatcca gaaacgaacg aaaaatatat tccatattgt
     1081 atcgagccat cacttggtgc agatcgtgta acattagctt tcttatgtga tgcatatgat
     1141 gaagaaggcg ttgaaggtag taaagatgca cgtacagttt tacacttcca tcctgcatta
     1201 gcaccatata aagcagcgat tttaccttta agtaagaaat tatctggcga agcgattaag
     1261 atttttgagc aattaagttc taaattctca atcgatttcg atgaatcaca atctatcggt
     1321 aaaagatacc gtcgtcaaga tgaaatcggt acaccttatt gtgtaacatt cgactttgat
     1381 tcattagaag ataatcaagt tacagtacgt gacagagatt caatggaaca agttcgtatg
     1441 ccaatctcag agttagaagc tttcttaact gaaaaaacaa aattctaatt aaaaagactt
     1501 tccaccatat gtggtggcta tataatttat aaaaagagat actggttagt aaaattgcca
     1561 gtatctcttt tctattcaaa aaggtattca aataaacaca tctatgtggc tacttgaata
     1621 ccttttatta ttttatgtta ttgttccaat ctttttaatt ggttgattag tttctgactt
     1681 ttaaaaaaca tacctgcata ttcacgatat aacattaaaa tgatatctga catttcatca
     1741 ataatttctt gatggatatt caatgaattc attttatcta tcggtaattt ttgtaataca
     1801 tctaataaat atagtgtttt attcgataat ataactgcat gtacatcttt agaagcctct
     1861 tgccttgaaa tcgcaccgtc aaacttaaaa ctataaccta ttaaatctgc ttgtgtgtca
     1921 ttaccactca cagcacagcg attaaatgat gcagtaaaac caaatcgttt catgcacttt
     1981 aacataacta cgactgacat taactgtgca gatgtacctg attctatttt ttcaagaaca
     2041 aattgtaata attgatagtt atatggtgca atgtcacctt catccattga gcgctcaata
     2101 gtttcagctg ccagagcggc ataactgctt acgtaaaggt ccatttgtaa tttataatgt
     2161 tgactaataa catctacaga atttaacgtt cccatacctc gccactgatt gtaaataaac
     2221 aaaccataaa caaacaattg cgtttgcgct tgtaaacccg tcttaacttt tttagcacgc
     2281 cttgccataa gtggtacttt tgcaccatgc tcatttaaaa tcgtgataat tttatcagat
     2341 tcaccataat caactgcttt gatgataatc cctttttggc gcattaacaa ttatcttcac
     2401 cacttttaag attaatcttg gtcttcaaca taaccaattt ggcgaataaa gttaactttg
     2461 tttcgccagt ctctttgaac tttgacccat aattctaagt aaactttaga gcctagaagc
     2521 atttctatat cacgtctcgc acgttttcct acttctttta actttttacc gccttttcca
     2581 atgacaattc ctttttgcga atctctttca acatatatag ttgcttcgat atgaacacga
     2641 tcttcgcttt ctttaaccat acggtccaca ttaacaccaa tcgcatgagg gatttcttca
     2701 cttgtaagat gaaggatttt ttcacgaatg atttcaccca ctacaaattg ttcaggatgg
     2761 tctgaaattt gatcatctgg ataatattta ggtccttcgg gtaaatacgt ctttaaaaca
     2821 tcaataaaat gatcgacatt tagcccttct aatgctgaaa taggtacaat ctctgtaaag
     2881 tccatataac tttgatattc ttcaatcttt ggcattaatt catctggatg cactaaatct
     2941 attttattta atactaaaaa tactggtgtc ttaacatttt tcaacatttc tataatatat
     3001 tcatcgcctc gtccaatttc ctcattggca ttaaccataa acatgattgc atctatctca
     3061 gataatgtat ttttagcgac tttcatcata tagtcaccta atttgtgttt aggtttatga
     3121 atacctggcg tatcaatgaa tataatttgc gcgtcatctc ttgtcataac accttgaatt
     3181 ttatttctag ttgtttgagc tttatcggac atgattgcta ttttatggcc gatcactcta
     3241 ttaacaaatg ttgactttcc tacatttggt ctacctataa ttgaaacaaa tcctgattta
     3301 tgttctgtca tttattctaa atcctttcct gaaaatccaa atggtagtaa ctctgcgact
     3361 gtcatcataa ccatatctcc tttatgattt gtcatataca caggcatatc atcatcacat
     3421 aattccttca aaacttgacg acatgcacca caaggtgatg acggtttatc tgcatctacg
     3481 gttacagtta ttgattcaaa atcacctggt ctgtatcctt gagaaattgc cgataccaaa
     3541 ctagctcgtt cagcacatat cgataatgga taagaagcat tttctacatt ggtaccataa
     3601 aaagttctac cgtctttcgt ttttaaataa gcccctactt taaattgact gtatggcgaa
     3661 tatgattctt gttgtgcttt tctaacttct tgaaaataat gaggttgata actcatatat
     3721 gcctccctaa aataacgcta taaaatgtgg taaaaatact attaaaccta taataaatgc
     3781 taatattgaa actataagta cactaaaagc cgcaatatct ttagcgtatt tagctaaatc
     3841 atgatattca acggtcacta aatcgacaac atattcaata gcagtgttta aagcttcaac
     3901 agtgagaact aatgcaatag caatgagtat aaatatccac tcaatccgat taatatttag
     3961 tacgagacca aagacaatag caacaatcat tgcaaacaca tgtaaaagaa atttatagtc
     4021 tttttgaatt aagattttca gcccatcaag tgcatattta aaccttttca taattagtct
     4081 cgtgttaatc catatgcgtt taatattgta tcttgtcgac caaacatttc cttttcatcc
     4141 gcttcagtca tatgatcata acctaataga tgcaaaaatc catgtaatgc taaaaatcct
     4201 aattctcgtt caaaagaatg tccgtaattg tttgcttgtt cttgcgctac atccgtacag
     4261 ataattatat cccctaaaac acgtggtata tcaagaccac taaaatcaat ctctggctca
     4321 tcttcttcta aagcaaatga gattacatct gtaactttat ctttatctct atatgttcga
     4381 ttaatttctt gtatttcttg tttatctaca aatgtaacag aaagctcagc atcgtcttct
     4441 atatgctctt cttttttagc aaattctaat aaatcttcaa tttgtttata ccaagcatct
     4501 ttaactaagc ctgtgtgatc gctaaaatct atcgtaaaca tttaattctc tccttcataa
     4561 tgttcaatga tcttacttac caatggatgt ctgactacat cgctctgatc taatttcaat
     4621 atacttatac ctttaacgtt gtgtaaccta ctgaccgctt ccttaagtcc acttttaaca
     4681 cctttaggta aatcgatttg agtttggtca ccagtaacta ccatttttga gccaaaacct
     4741 agtcttgtta aaaacatttt catttgcgca tgtgtcgtat tctgcgcctc atcaagaatt
     4801 acaaatgcat cttctaatgt tcgtccgcgc atatatgcaa gtggcgctat ttcgataatg
     4861 cctctttcaa taaatcgctc tgtttgttca cgcccaagaa cagtatatag accatcatat
     4921 aaaggtctta aatatggatc taccttttct ttcaaatctc ctggtaaaaa tccaagtgac
     4981 tctcctgctt caacagcagg tcttgttaat acaatacgtt taacagcacc tttacggagt
     5041 tgctttgctg cataaactac agctaagaat gtcttacctg taccagcagg acctatacca
     5101 aatactaaat cattattttt catggcatta acatatatac gttgccccat cgttttcgca
     5161 cgaatcgtct taccaaatgc atctttagtt atctcttcat catataaatc taacagatgt
     5221 tgaattgtgt tattatgcgc cattttaata gctgcttcaa catctttaat tgtaatatta
     5281 ttacctaaat caataacctt cagcaaattg attaatactg attccgcttt ttctacgttt
     5341 tctatttttg tacctttaac ggcaacttct tgtcctcttg catggatgac aacatcgaaa
     5401 ctctcttcaa ttgcttttaa atgttcatca ttatttccaa ttaaagcttg agattggttc
     5461 atatcgtcta tttgtataat tccaggcata cacgcgctcc ttttcatata tcatttatat
     5521 tcattctatg atgctttaat atctacttaa ttatatcatg caattgattg atttaaacat
     5581 ctttgcattg
//
LOCUS       DAXWFK010000156         5489 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.157, whole genome shotgun sequence.
ACCESSION   DAXWFK010000156 DAXWFK010000000
VERSION     DAXWFK010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5489
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.157"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            95..2713
                     /gene="mutS"
                     /locus_tag="ACGEXS_002227"
     CDS             95..2713
                     /gene="mutS"
                     /locus_tag="ACGEXS_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFF8013217.1"
                     /translation="MSNVTPMMQQYLKIKSEYQDCLLFFRLGDFYEMFYEDAKEASRV
                     LEITLTKRDAKKENPIPMCGVPYHSADSYIDTLVNNGYKVAICEQMEDPKQTKGMVRR
                     EVVRIVTPGTVMEQGGVDDKQNNYILSFVMNQPEIALSYCDVSTGELKVTHFNDEATL
                     LNEITTINPNEVVINDNISDNLKRQINMVTETITVRETLSSEIYSVNQTEHKLMYQAT
                     QLLLDYIHHTQKRDLSHIEDVVQYAAIDYMKMDFYAKRNLELTESIRLKSKKGTLLWL
                     MDETKTPMGARRLKQWIDRPLISKEQIEARLDIVDEFSAHFIERDTLRTYLNQVYDIE
                     RLVGRVSYGNVNARDLIQLKHSISEIPNIKALLNSMNQNTLVQVNQLEPLDDLLDILE
                     QSLVEEPPISVKDGGLFKVGFNTQLDEYLEASKNGKTWLAELQAKERQRTGIKSLKIS
                     FNKVFGYFIEITRANLQNFEPSEFGYMRKQTLSNAERFITDELKEKEDIILGAEDKAI
                     ELEYQLFVQLREEVKKYTERLQQQAKIISELDCLQSFAEIAQKYNYTRPSFSENKTLE
                     LVESRHPVVERVMDYNDYVPNNCRLDNETFIYLITGPNMSGKSTYMRQVAIISIMAQM
                     GAYVPCKEAVLPIFDQIFTRIGAADDLVSGKSTFMVEMLEAQKALTYATEDSLIIFDE
                     IGRGTSTYDGLALAQAMIEYVAETSHAKTLFSTHYHELTTLDQALPSLKNVHVAANEY
                     KGELIFLHKVKDGAVDDSYGIQVAKLADLPEKVISRAQVILSEFEASAGKKSSISNLK
                     MVENEPEINQENLNLSVEETTDTLSQKDFEQASFDLFENDQESEIELQIKNLNLSNMT
                     PIEALVKLSELQNQLK"
     gene            2726..4735
                     /gene="mutL"
                     /locus_tag="ACGEXS_002228"
     CDS             2726..4735
                     /gene="mutL"
                     /locus_tag="ACGEXS_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFF8013218.1"
                     /translation="MGKIKELQTSLANKIAAGEVVERPSSVVKELLENAIDAGATEIS
                     IEVEESGVQSIRVVDNGSGIEAEDLGLVFHRHATSKLDQDEDLFHIRTLGFRGEALAS
                     ISSVAKVTLKTCTDNANGNEIYVENGEILNHKPAKAKKGTDILVESLFYNTPARLKYI
                     KSLYTELGKITDIVNRMAMSHPDIRIALISDGKTMLSTNGSGRTNEVMAEIYGMKVAR
                     DLVHISGDTSDYHIEGFVAKPEHSRSNKHYISIFINGRYIKNFMLNKAILEGYHTLLT
                     IGRFPICYINIEMDPILVDVNVHPTKLEVRLSKEEQLYQLIVSKIQEAFKDRILIPKN
                     NLDYVPKKNKVLHSFEQQKIEFEQRQNTENNQEKTFSSEESNSKPFMEENQNDEIVIK
                     EDSYNPFVTKISESLIADDESSGYNNTREKDEDYFKKQQEILQEMDQTFDSNDGTTVQ
                     NYENKASDDYYDVNDIKGTKSKDPKRRIPYMEIVGQVHGTYIIAQNEFGMYMIDQHAA
                     QERIKYEYFRDKIGEVTNEVQDLLIPLTFHFSKDEQLVIDQYKNELQQVGIMLEHFGG
                     HDYIVSSYPVWFPKDEVEEIIKDMIELILEEKKVDIKKLREDVAIMMSCKKSIKANHY
                     LQKHEMSDLIDQLREAEDPFTCPHGRPIIINFSKYELEKLFKRVM"
     gene            4741..5283
                     /locus_tag="ACGEXS_002229"
     CDS             4741..5283
                     /locus_tag="ACGEXS_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFF8013219.1"
                     /translation="MNQVNNNILPAIRNIKDLEKLIKTDYKMCVLLDMHIGHIKSIME
                     LLKQNHIECFIHIDLIKGLSHDEFASEFIIQQYKPKGIVSTKSKVIKKAKSLNTLTIF
                     RVFIIDSQALKRSIDLIKKVEPDFVEVLPGVASKAIHHIQKETNTQVIAGGLINTIDE
                     VNEAVKNGAKYVTTSYDKLW"
BASE COUNT         2190 a          710 c          996 g         1593 t
ORIGIN      
        1 tgtataagtt aaataattaa taattaaaaa gcttacaaac gaatatattg taaagacaaa
       61 ttttaaaaag agacaagggt tgtgaagaaa taatatgtct aatgttacac caatgatgca
      121 gcaatattta aaaataaaat cagaatacca agattgctta ttatttttta gactaggtga
      181 tttctatgaa atgttttatg aagatgccaa ggaggcatca cgtgtacttg aaattacttt
      241 aactaaaaga gatgctaaaa aagaaaatcc aattccgatg tgtggtgttc cgtatcattc
      301 tgcagatagt tatatagata cacttgttaa taatggatat aaagtagcta tttgtgaaca
      361 gatggaagat ccgaaacaaa cgaaaggtat ggttagacgt gaggtagtaa gaattgtgac
      421 tccaggaact gtgatggagc aaggtggtgt agatgataaa caaaataact atattttaag
      481 ttttgttatg aatcaacctg aaattgcgct tagttactgt gatgtttcta ctggcgaatt
      541 aaaggttaca cattttaatg atgaagcgac tttattaaat gaaattacga cgataaaccc
      601 taacgaagtt gttatcaatg acaatatttc cgataattta aaaagacaaa ttaatatggt
      661 gacagaaaca ataacagtca gggaaacgtt atcatcagaa atctatagtg tgaatcaaac
      721 tgaacataaa ttaatgtatc aagcgacaca attattgcta gattatattc atcatacaca
      781 aaaacgtgat ttatcgcata tcgaggatgt tgttcaatat gcagctatag attatatgaa
      841 aatggatttt tatgctaaga gaaaccttga gttaacggaa agcattcgat taaaatcaaa
      901 aaaaggaacg ctactttggc taatggacga aacgaaaaca ccaatgggag cacgccgctt
      961 aaaacaatgg atagatagac cactaataag taaagaacaa attgaagcac gattagatat
     1021 cgttgatgaa tttagtgctc atttcataga aagagacacc ttaagaacat atcttaatca
     1081 agtgtatgat attgaacgtc ttgttgggcg tgttagttac ggaaatgtta atgcgagaga
     1141 tttaattcaa cttaaacatt ccatttctga aataccgaat attaaagcat tactaaattc
     1201 tatgaatcag aatactcttg tacaagttaa tcaactagaa ccccttgatg atttacttga
     1261 tatattagaa cagagtttag tagaagaacc accaatttca gttaaagatg gcggactatt
     1321 caaagttggt tttaatacgc aattagatga atatcttgaa gcttcaaaaa acggaaaaac
     1381 atggttagca gaattacaag ccaaagaaag acaacgtaca ggaataaaat cattgaaaat
     1441 aagctttaat aaagtgtttg gttattttat agaaataaca cgtgccaact tgcaaaattt
     1501 tgaaccaagt gaatttggtt atatgaggaa gcaaacgtta tcgaatgctg aacgttttat
     1561 aactgatgaa cttaaagaaa aagaagatat cattttaggt gcggaagaca aagccatcga
     1621 attagaatat caattatttg ttcagctacg tgaagaagtt aaaaaatata ctgaacgttt
     1681 acaacaacaa gctaaaatta tttcagagct agattgttta cagagctttg cagaaattgc
     1741 tcaaaaatat aattacacta ggccttcatt tagtgaaaat aaaacattag aattagtgga
     1801 atctaggcac ccagtagtgg aaagagtaat ggattataat gactatgtgc ctaataattg
     1861 tcgattagat aatgaaacat ttatatattt aattacaggt ccgaatatgt ctggtaaatc
     1921 gacatatatg agacaagttg ccataattag tataatggcc caaatgggag cttatgtccc
     1981 ttgtaaagag gcagtgttac ctatatttga tcaaatattc actagaatag gtgcggcaga
     2041 tgatttggtt tcaggtaaga gtacgtttat ggtagaaatg ctagaagcac aaaaggcatt
     2101 aacttatgca acagaggata gtttgattat tttcgatgaa attggacgtg gtacttcaac
     2161 gtatgacggt ttagctttag cacaggcaat gatagagtat gtagctgaaa catcacatgc
     2221 taaaacgtta ttttcaacac attatcatga attgacaaca ttagatcaag cattaccaag
     2281 tctaaaaaat gttcacgtcg ctgctaatga atataaaggt gaacttatat tcttgcataa
     2341 agtcaaagat ggtgcagttg acgatagtta tggtattcaa gttgcgaaat tagctgattt
     2401 acctgaaaaa gttattagca gagcacaagt gattctaagc gagtttgaag cgtctgctgg
     2461 taaaaaatca tcgatatcaa atttaaaaat ggtcgaaaat gaacctgaaa ttaatcaaga
     2521 aaatttaaac ttaagtgttg aagaaacaac tgatacttta tctcaaaaag actttgaaca
     2581 agcatcattt gatttgtttg aaaatgatca agaaagcgag attgaactac aaattaaaaa
     2641 tttgaattta tctaatatga caccaattga ggcattggtg aagttaagtg aattacaaaa
     2701 tcaattaaaa tagaggtgtt gcaaaatggg gaaaattaaa gaactccaaa cctcattagc
     2761 aaataaaatc gcagcaggtg aagtagttga aagaccgagt tctgttgtga aagaactgtt
     2821 ggaaaatgct atagatgcag gcgctacaga aataagcatt gaagtagagg aatctggcgt
     2881 ccaatctatt cgcgtagtcg ataatggaag cggaattgaa gcggaagact taggattagt
     2941 atttcataga catgcgacta gtaaattaga tcaagatgaa gatttatttc atattaggac
     3001 attaggattc cgtggtgaag cactagccag tatttcatca gttgctaaag taacattgaa
     3061 gacttgcacg gataatgcta atggaaatga aatatatgta gaaaatggtg aaatattaaa
     3121 tcataagcct gcaaaagcga aaaaaggaac agatatactt gtagaatcat tattttataa
     3181 tacaccagca cgtttaaaat atattaaaag tttatacact gaactaggta aaataacaga
     3241 tattgtcaac agaatggcaa tgagccatcc ggacattcga atagcactca tttcagatgg
     3301 caaaacaatg ttaagtacaa atggttcagg acgaactaat gaagtgatgg cagagattta
     3361 tgggatgaaa gttgcacgag atttagtaca tatatctgga gatacaagtg attatcacat
     3421 tgaaggtttt gttgcaaagc ctgaacattc tagaagtaat aagcactata tttctatttt
     3481 tattaatgga cgatacatta aaaactttat gctaaataaa gcgattttag aaggctatca
     3541 tacactctta acaataggta ggttcccgat ttgttatatt aatattgaaa tggatccaat
     3601 cttagtagac gtaaatgttc atccaacaaa actagaagtg cgtttatcaa aagaagagca
     3661 actataccaa ttgattgtga gcaaaataca agaagcattt aaagaccgta tattaattcc
     3721 taaaaataac ttggattatg tgccaaaaaa aaataaagta ttacattcat tcgaacaaca
     3781 aaaaatcgaa tttgaacaaa gacaaaacac agagaataac caagagaaga cgttttcatc
     3841 tgaagaaagt aacagtaagc catttatgga agaaaatcaa aacgatgaga tagttataaa
     3901 agaagattca tataatccat tcgtaacgaa aatatctgaa agtttaatag ctgatgatga
     3961 atcttctggt tataataata cacgtgaaaa agatgaagac tacttcaaaa aacaacaaga
     4021 aattttacaa gaaatggatc aaacatttga ttcaaatgat ggtacaactg tgcaaaatta
     4081 tgagaataaa gcgtctgatg attattatga tgtaaacgat attaaaggaa caaaaagtaa
     4141 agaccctaaa cgaagaattc catatatgga aattgttgga caagtacatg gaacgtatat
     4201 tattgctcaa aatgaatttg gcatgtacat gattgaccag catgcagctc aagaaagaat
     4261 aaaatatgaa tattttcgag ataaaatagg tgaggttacc aatgaagtac aagatttatt
     4321 aatcccgtta acatttcatt tttcaaaaga tgaacaatta gtcattgatc aatataaaaa
     4381 tgagcttcaa caagtaggta tcatgttaga acattttggt ggtcatgatt atattgtaag
     4441 tagctatcca gtttggttcc ctaaagatga agtagaagaa attattaaag atatgattga
     4501 gctaattttg gaagagaaaa aagtagatat caaaaaatta cgtgaagatg tagcaatcat
     4561 gatgtcatgt aaaaaatcca ttaaagcgaa tcattattta caaaaacatg aaatgtctga
     4621 tttaattgat caattaagag aagcggaaga tccatttaca tgtccacatg gtcgtccaat
     4681 aatcattaat ttttcaaaat acgaattaga aaaattattt aagcgtgtga tgtagagagg
     4741 atgaatcaag tgaataacaa catattgcct gctataagaa acattaaaga tttagagaaa
     4801 ctgattaaaa cagactataa aatgtgtgtg cttctagata tgcatatagg acatataaaa
     4861 agtattatgg aattgctgaa gcaaaatcat atagagtgtt ttattcatat agatttgata
     4921 aaaggtttaa gccacgatga atttgcaagt gaatttatta ttcagcaata caagccaaaa
     4981 ggtatcgtat cgactaaatc taaagtaata aaaaaagcta aatcattaaa tactttaacg
     5041 atttttagag tatttattat tgatagtcaa gcattgaaac gcagtataga tttgataaaa
     5101 aaagttgaac ctgattttgt tgaagtactt ccaggtgttg cgagtaaagc gattcatcat
     5161 attcagaaag aaacaaacac acaagtcatt gcaggtggcc taattaatac aatagatgaa
     5221 gtcaatgaag ctgttaaaaa tggagcgaaa tatgtaacta ctagttatga taaactttgg
     5281 taaatcataa gtctgcaaaa atttcatttt tcacgagatt tagtcatata acataataaa
     5341 aaagccgttt tcatatggga tatgcagtag gtatcaaaca aaaaataatc aaataaaata
     5401 attagcacct ctaaaacgat taaaatcatt aaagttttag aggtgtttat tagtaaaaaa
     5461 tgcaactagt cataaaaaat atggctgaa
//
LOCUS       DAXWFK010000157         5449 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.158, whole genome shotgun sequence.
ACCESSION   DAXWFK010000157 DAXWFK010000000
VERSION     DAXWFK010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5449
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.158"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(3..1565)
                     /locus_tag="ACGEXS_002230"
     CDS             complement(3..1565)
                     /locus_tag="ACGEXS_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HFF8013220.1"
                     /translation="MKNKSVSLVFWVSLVICTIFVAFGAIFPKQLEKLTQNITTFIAL
                     HFSWYYLLLVLVILFVCVYILFSRYANITLGEEGEDPEFSLPSWFAMLFSAGMGIGLV
                     FWTTAEPISHAFKLTPIHKAGTQSAINDAMQFSFFHWGIHAWAVYGIVALVFAYFSFH
                     KGYLGLVSATLTPLLGEKAMRGPLGGAIDVLAVIATVTGVAATLGFGALQINEGLHFL
                     FNVPSNFTMQVILIVIATILFTWSAWSGIDKGIKTLSNINMLLAFVVLIGLFIVGPTL
                     YILNTFTNGLGNYIANFFSMSLRIPTGGQKFQWLQNWTIFYWAWWISWAPFVGIFIAR
                     VSKGRTIKEFILGVLFVPALVCFIFFAVFGASAIYLQDNHIADIAKAATETATFATLQ
                     HYPLGFVLSLITLLVIMIFFVTSADSATYVLGMLSSSGDINPKSFVKVSWGIIMALFA
                     IIMIYTGGTQAIQNLLIIAALPFSVVIIAMIWSLIKSLSEEKPRNSNKVLIKHRDPDI
                     LEYRSSKHIDEN"
     gene            complement(1872..2874)
                     /locus_tag="ACGEXS_002231"
                     /pseudo
     CDS             complement(1872..2874)
                     /locus_tag="ACGEXS_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500913.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
     gene            complement(3108..4109)
                     /locus_tag="ACGEXS_002232"
     CDS             complement(3108..4109)
                     /locus_tag="ACGEXS_002232"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFF8013221.1"
                     /translation="MQNKQILFNKIPEGMPQEDTFKIEEIDTPKLESDGVLVQTLYIS
                     VDPYMRGRMTKADSYVQPFEIGKPIVSHVVAKVIDSTLADYKKGDVVVGMLPWRIINH
                     VQADQITKVPTTDVPLDLYLSVLGMPGQTAYHGLLDIGQPKAGDTVVVSAASGAVGSV
                     VGQIAKIKGCRVVGIAGGDKKVNYLTETLGFDAGIDYKKEDFAEALAQAVPDGIDVYF
                     ENVGGTVGDEVFKHLNRFARVPVCGAISSYNHPEADIGPRIQGTLIKKQVMMRGFLVS
                     EFANAFKEASEQLATWVQEGKIQSQVTIEDGFENAPHAFKNLFTGDNFGKQVIKVTE"
     gene            complement(4392..5261)
                     /locus_tag="ACGEXS_002233"
     CDS             complement(4392..5261)
                     /locus_tag="ACGEXS_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFF8013222.1"
                     /translation="MKKIFVIITTLLAVAIIIGSIIMVVFSQRQAQTFKIQQQQFVKK
                     PIPTLFLHGFGGSANSEKFMVKQAEKRGVTKDIITAYVSKDGAVTFKGKLRKDAVNPI
                     VKIELENNRQGYLDKNAAWFKNVLTKLQSEYNFDKFNFVGHSMGNLTFAQYMMTYGND
                     KSLPQLNKQVNIAGTFNGVLNMNEDVNEITVDKDGKPSRMNQPYQQLRVLKDIYKGKG
                     IEVLNIYGDLKDGTHSDGRVSNSSSKSLKYLLGNSPKSYRESKYEGEPAQHSQLHENE
                     NVANELIDFLWKK"
BASE COUNT         1732 a         1067 c          791 g         1859 t
ORIGIN      
        1 aattaatttt cgtcaatatg ttttgaagaa cgatattcta aaatatctgg gtcacgatgt
       61 ttaattaaaa ccttattact atttctcggt ttctcctcac tcaaagattt tataagcgac
      121 catatcatcg ctataatgac cacggaaaat ggtaacgcag caatgattaa taaattttga
      181 attgcttgag taccacctgt gtaaatcatg atgattgcaa ataatgccat aatgataccc
      241 caactcactt tgacaaatga cttcggatta atatcaccac ttgaactcaa catacctaaa
      301 acataagttg ccgaatccgc tgatgtaaca aagaaaatca taataacaag taaagtaatt
      361 aagcttaata caaaacctag cggataatgt tgtagcgtcg caaaagttgc tgtttctgtc
      421 gcagctttag caatatcggc aatatgatta tcttgtaagt aaattgctga cgcgccgaat
      481 accgcaaaga atataaagca aactaacgcc gggacaaaaa gtacacctag aataaattct
      541 ttaatcgtac gtccttttga cacacgtgca ataaatatac ctacaaatgg tgcccaagat
      601 atccaccatg cccagtaaaa gattgtccaa ttttgtaacc attggaattt ttgaccacct
      661 gtcggaatgc gtaaactcat actaaagaaa tttgcaatat aattacctag accattcgta
      721 aatgtattta aaatgtatag cgttggccca acaataaaaa gaccaataag tactacaaaa
      781 gcaagtaaca tgttgatatt actcaacgtt ttgatacctt tatcgatacc tgaccatgct
      841 gaccaagtaa ataatatggt tgcaatgaca atcaagatta cttgcatcgt gaagttactc
      901 ggtacattaa ataaaaaatg taaaccttcg tttatttgca atgcaccgaa acctaatgtt
      961 gcagcaacgc ctgttactgt agctatgact gccaatacat caatggcacc acctaaaggt
     1021 ccacgcattg ccttttcacc taataaaggc gtcaatgtag cgctgactaa gccaagatat
     1081 cctttatgaa agctaaaata tgcaaacact agcgcgacaa taccatagac tgcccatgca
     1141 tgaatccccc aatggaaaaa tgaaaactgc attgcatcat taattgcaga ttgcgtgcca
     1201 gctttatgaa taggcgttaa tttgaaggca tgactgattg gttctgccgt tgtccagaac
     1261 acaagtccta ttcccatacc agcactaaat aacatagcaa accaagacgg caatgagaat
     1321 tcaggatctt cgccttcttc acctaatgta atgtttgcgt atctcgaaaa taaaatatac
     1381 acacagacaa ataaaataac taaaacgagc aataaataat accaagaaaa atgtagcgca
     1441 ataaatgtag taatgttttg cgtgagtttt tctaactgtt tcggaaatat tgctccaaaa
     1501 gcaacaaata tcgtacatat cactaaagat acccaaaaca ctagacttac tgatttattt
     1561 ttcataaata caaacccttt ctgtgtaatg gtaagttcat acccataact gcaacatttt
     1621 aatcatttgt aattttatat agacacaatt aataatgcct catcttttaa aaatgatata
     1681 taaaacacac tcaaattatt tatcattgag caacaaagta ttttattgta tttaagtaat
     1741 gcctttctag tgcattattg atttgatacc tgcaaagttg ccatatttcc gtttagaatc
     1801 aatagtcgct agacacaaaa aataagtcgc ctatacagta ttttctgcat aaggcgactt
     1861 tacttactaa tctatatatt aattactaat tttccaatca ttgattgttt ttccaacaat
     1921 tgatgtgctt gatataagtt ttcaggtgat aaaccttcaa aaacttgtgt cgttgttggt
     1981 tggtaatgcc ctgattctat atttttcgta atatcttcta aatactcatg ttgtttaatc
     2041 atatcaggcg ttcgatgaat tggacgcgca aacataaatt catgtgtaaa tgttatactt
     2101 tttaatttta atgcatttaa atcttgatct tcattaaaag ctacgatagt cgtaatatgc
     2161 cctaatggtt ttatcagttc aatcatagta ttgtaataca agtctgtatt ataggtgcaa
     2221 aatatataat ctactaatgg aatttcttta aattgacgca ctaaatcctc tttatgattc
     2281 aatacgatat ctgcgcccat cttttcacac cactctgttg tttcttgtcg tgatgctgtt
     2341 gtaatgacag ttaaaccata ccgtttagca atttgagtgg ctatactgcc tacaccaccg
     2401 gcaccattaa tgattaagac agacttccct tcgttttcag caggattcgt agaaatttta
     2461 aatgtatcaa aaaaacgttt catatgccgt aataccagtt agcggtagac taaccgcttc
     2521 attagcactt atgttgtgtg gtgcttttgc aactatagct tctgacacca attgatatgt
     2581 cgcatttgat ccttgtctat ttggcgatcc agcataaaat acaacgtcac ccggactaaa
     2641 taatgtaacg tctggtccga tagcttcaac agtaccaata gcatcaaacc caagtacacg
     2701 aggtgcttga gtgacttcca tttgtcgttg ctttgtatct acaggattta cactaatgct
     2761 atttactttg actaagatat catcgttttc tggcgttggc tttctttgtt cgtatacttt
     2821 aaataaattt ccttcttcca atttaaaggg cttctcaaat cctatcattt tcatatcgtt
     2881 tcacctcatt tatgaactta tttcttatta tacaaaatag aagccatgtg tgcttatatc
     2941 gcagcatcat gactcctttt tcatttgaat atataaataa ttacagacga ctttcgtatt
     3001 aaattttaga cttatttcta ccatgttgct gaacaaattt actttagata aaaaattatt
     3061 aaattttggt caattaacaa agttagtttg ttaaaacgta tactttatta ttccgttact
     3121 ttaataactt gtttaccaaa gttatcgcca gtaaataaat ttttaaatgc atgtggcgca
     3181 ttttcaaaac catcttcaat ggttacttgt gactgaattt taccttcttg aacccatgtt
     3241 gcaagctgtt cactagcttc tttaaaagca ttagcgaatt cacttaccaa gaagcctctc
     3301 atcattactt gcttcttaat aagcgtacct tgaatacgtg gtccgatatc ggcttcagga
     3361 tgattatatg acgaaattgc gccacatact ggtacacgtg caaaacgatt taaatgctta
     3421 aatacttcat cgccaactgt tccaccaaca ttttcaaaat aaacatcaat accatctggt
     3481 actgcttgtg ctaacgcttc tgcaaaatcc tctttcttat aatcaatacc agcgtcaaag
     3541 cccagtgtct ctgttaaata atttactttt ttgtcgccac ccgcaatacc tactacacgg
     3601 caacctttaa tcttagcaat ttgacctaca actgaaccta cagcaccaga tgcagctgaa
     3661 accacaacag tatcaccggc tttaggttgt ccaatatcaa gcagaccatg atatgctgtt
     3721 tgtcctggca ttcctaaaac acttaaatat aaatcaagtg gtacatctgt cgttggaact
     3781 ttagtaattt gatccgcttg gacatgatta atgattcgcc aaggcaacat acctacaacg
     3841 acatctcctt ttttataatc tgcgagtgtc gaatcaatta cttttgcaac gacatggcta
     3901 acaatcggtt taccaatttc aaaaggctgt acatacgaat ctgccttagt catacgtcct
     3961 ctcatatatg gatccactga aatatacagc gtttgtacaa gtacaccatc gctctcaagt
     4021 ttaggcgtgt caatctcttc aattttgaat gtatcctctt gaggcatgcc ttctggtatt
     4081 ttgttaaaaa gaatttgttt attttgcatc attaatcacc tttctttatt tgaaactttt
     4141 acttatttgt tacttaagcg ttaagtttga attgtgtctt cgtgatgtct gtatgcaaat
     4201 acattcttag ttgttatatt ttgacttaag cactgattca ttcatgtaac ttcaaccaca
     4261 ttatatttgc tataatcata aatttaaaat gttacgactt agacatttta tggaaactct
     4321 caaacaatag ataatttttg aaaagctcta atattacaag cttttttgcc ccagaaaaac
     4381 tagcagttgc tttatttttt ccataagaag tcgattaact cattagcaac attttcattc
     4441 tcatgaagct gactatgttg tgcaggctca ccttcatatt tagattctcg ataacttttc
     4501 ggactatttc ccagtaaata ttttaatgat ttcgaagaac tattagacac tctgccgtct
     4561 gaatgtgtac catctttcaa atccccataa atatttagca cttcgatacc tttaccttta
     4621 taaatatctt ttagcacgcg taattgctga taaggttgat tcattcgact tggtttacca
     4681 tctttatcaa ctgtaatttc attgacatct tcattcatat ttaaaacacc attaaatgtc
     4741 cctgcaatat tcacttgttt gtttaactgt ggcagtgact tgtcgttacc atatgtcatc
     4801 atatattgtg caaatgttaa gttccccatt gagtgaccga caaagttgaa tttatcgaaa
     4861 ttgtattcag attgtaactt agtcagtaca tttttaaacc acgcagcatt cttatccaaa
     4921 tagccttgtc tgttattttc aagttcaatt ttcacaatag gattcactgc atcttttctt
     4981 agtttccctt taaatgtcac tgcaccatcc tttgaaacgt aagcagtgat gatatcttta
     5041 gttacccctc ttttttctgc ttgcttcacc ataaactttt cagaattggc actaccacca
     5101 aatccatgta agaacaatgt tggaattggc tttttaacaa attgctgttg ttgtatttta
     5161 aatgtttgtg cctgtcgttg actaaacacc accataataa tagagcctat aataatagcg
     5221 accgctaaca atgtcgtaat aattacaaaa attttcttca cacttttaac tcccattcat
     5281 gtcttttata taagtataaa ggatgtgatt aaaaatgtcc tttagttgat tttgaataca
     5341 tcattaactt ttaagatgac tttggaaagt tgtccgttaa cgtttgttaa ttgattgctt
     5401 ctttagcttt caatggtgtg tcacccattg attaatatat aaatatgta
//
LOCUS       DAXWFK010000158         5435 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.159, whole genome shotgun sequence.
ACCESSION   DAXWFK010000158 DAXWFK010000000
VERSION     DAXWFK010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5435
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.159"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            56..514
                     /locus_tag="ACGEXS_002234"
                     /pseudo
     CDS             56..514
                     /locus_tag="ACGEXS_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226788.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            501..803
                     /locus_tag="ACGEXS_002235"
     CDS             501..803
                     /locus_tag="ACGEXS_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013223.1"
                     /translation="MRTNDHITDLDAIELSSGWNYNSKLETGKNALLSDSLLRDIKVL
                     SSNAKGDADILETELRAVINSMSQNEGKSVDALKNSTASLKNNLTKLKEQLDKTTH"
     gene            957..1316
                     /locus_tag="ACGEXS_002236"
     CDS             957..1316
                     /locus_tag="ACGEXS_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013224.1"
                     /translation="MILRYATNLKLAKELYQASKPKLQEDKGMIELFEHTFGLQKELV
                     KYVGIAEATTAALYSASFINKNISRLASLSTIGILSVAAYKHFEAGQGKKGAQHALNL
                     LGLATLSLLDTFSCNKK"
     gene            complement(1401..2012)
                     /locus_tag="ACGEXS_002237"
     CDS             complement(1401..2012)
                     /locus_tag="ACGEXS_002237"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFF8013225.1"
                     /translation="MYTFDAQSLTARENYKLLIGSIIPRPIAFVTTLNQDASVNAAPF
                     SFFNIVNNHPPMIAIAVQRAGGKRKDTALNIERTGDFVVHITDEDNVQDINETAAPLA
                     YGDSELSRTELSLLTSTTIKTPGIKDAKMRFECKLSQMILLGDVLDGADLIIGEIVTY
                     HIDDSIYEGDFKINPHALQAVSRLAGNDYAKLGGIFTIKRPEK"
     gene            complement(2091..2567)
                     /locus_tag="ACGEXS_002238"
     CDS             complement(2091..2567)
                     /locus_tag="ACGEXS_002238"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HFF8013226.1"
                     /translation="MAVVYVAGGCLWGVEAFFATIPGIIHTEAGRANGRSSKLDGPYD
                     GYAECVKLHFDDRMLTITDIMNYLFEIIDPYSVNQQGNDIGQKYRTGLYSCVDDHLIA
                     ARQFIERRKDRDQIAVEVLPLSNYIKSAEEHQQHLEKYPEDMHMCHISKDLLNKYK"
     gene            complement(2570..3070)
                     /locus_tag="ACGEXS_002239"
     CDS             complement(2570..3070)
                     /locus_tag="ACGEXS_002239"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8013227.1"
                     /translation="MVLRKVIMQDLDQIIALENIGFSPEEAATREALKLRIEQIQETF
                     IVAEKNDEVIGYINGPVIKERYISDDLFKNVSTNNSEGGYISVLGLVVAPNYQGQGIA
                     GRLLNYFETLAKNHHRHGVTLTCRESLISFYEKYGYRNEGVSESCHGGIKWYNLVKNI
                     KKDDEV"
     gene            complement(3305..4072)
                     /locus_tag="ACGEXS_002240"
     CDS             complement(3305..4072)
                     /locus_tag="ACGEXS_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HFF8013228.1"
                     /translation="MIDIHNHILVDIDDGPKTIEKSIALLKQAKDEGVTSIVATPHHL
                     HPRYDNTFQQVLVKLAELRTHPEVQALDIKLFPGQEIRITDSILKGLDNGSIQGINRS
                     KYLLIEFPTGEVPHYTKQLFFEIQSRGYIPIIAHPERNRSIAKNPEILYELVANGALS
                     QLTSSSLVGGFGKNIQKLSLQFIECNLAHFVASDAHSCDQRPFLMQELFHNHKLKKYS
                     NDIEALLRNASSVINDNFVYLDRPTKPGKVKSFLKWF"
     gene            complement(4069..4761)
                     /locus_tag="ACGEXS_002241"
     CDS             complement(4069..4761)
                     /locus_tag="ACGEXS_002241"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HFF8013229.1"
                     /translation="MTNTRRSTSSLIVHEQPKSPISEKFRGIRSNIMFANPDSAVQSI
                     VITSEAPGAGKSTIAANLAVAYAQAGYKTLIVDGDMRKPTQHYIFNLPNNEGLSSLLL
                     NWSTYQDSIISTEIQDLDVLTSGPIPPNPSELITSRAFANLYDTLLMNYNFVIIDTPP
                     VNTVTDAQLFSKFTGNVVYVVNSENNNRDEVKKGKELIEATGAKLLGVVLNRMPKDKS
                     ASYYAYYGTDES"
     gene            complement(4778..>5435)
                     /locus_tag="ACGEXS_002242"
     CDS             complement(4778..>5435)
                     /locus_tag="ACGEXS_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFF8013230.1"
                     /translation="EKFDLVKLLNILKKNIKLLLILPAICLVVSAALTFFVMPDKYTA
                     STQILVNMKKSSSDLAFQNVQSSLQSVNTYTEIIKSPRILDKVSREFDGQYSTAELNS
                     FLKVTNQTNSQIITVSVTTGNKSESDKIVNKISKVFAHDMPKIMSVDNVTILSSAHDN
                     AVKVSPIVSVNLVISIIVGIVLAILIIFLKELLDKRIKTEEDVESQLGLPILGSIQKF
                     "
BASE COUNT         1813 a          971 c          780 g         1871 t
ORIGIN      
        1 tttgtaaatt taattaatta tagttacttc aatcatgatt agtttataat aataaagtga
       61 aattgaaaaa gacagctatt atgcgatgag cgaaaaactt caagtaaaac aagaatttaa
      121 cggcgttaca aggcaatact tgaatttcag aacagatagt atcgatgatc tggataagtt
      181 caaaaaagaa ttcaaacctg ttatgcactt aaaaggtgat gcattcaatc aacagttaca
      241 atctttgatt aacaaatatc cacaaataca aaaaaatatg aaatcagagt tcattgctta
      301 ttatgataaa gaaaaaaata gagaaacagt aaaaaactat gcttggaacc ttcaaaaatc
      361 tataaatgac attatgcaat catatcctag cacaaaattt gtacagtttt ataaagatga
      421 tgtttccccg tcaatggtag atggaaatgg ccgtttaaaa tcggatacta atgtaatttc
      481 catcgaggga ggaaagtatg atgagaacaa atgatcatat aaccgattta gatgctattg
      541 agttaagtag cggttggaat tacaattcga aattggagac tggcaaaaat gcactactat
      601 cagatagcct tttaagagat attaaagtat taagttctaa tgctaaaggc gacgctgaca
      661 tactagaaac agaacttcgt gctgtaataa actcaatgtc tcaaaacgaa ggtaaatctg
      721 ttgatgcact taaaaatagt accgcttctc taaaaaacaa tctaaccaaa ttaaaagagc
      781 aactcgacaa aactacacat taatttcata cattttgagt gaacagaatt atatttccat
      841 agcaaacatt cctaaactac acatacgtta cataattgat tcatttttat agaaacgggt
      901 aaaaatgata aagtaatcat ctttcacaaa aaatcaaata ttgggaggaa ttaaatatga
      961 ttttgcgtta cgcaacgaat ttaaaacttg cgaaagagct ataccaagcc agtaaaccta
     1021 aattgcaaga agataagggt atgattgaac tgtttgaaca tacttttggc ttgcaaaaag
     1081 aacttgtaaa atacgtcggc attgcagaag caactactgc tgctttatac tcagctagct
     1141 ttatcaataa aaacatttct cgccttgcat cactatcaac gataggcatt ttaagtgtag
     1201 ctgcttacaa acatttcgaa gctggccaag gtaaaaaagg tgcacaacat gcattaaatt
     1261 tattaggtct tgctacttta agtttactag atacattttc ttgtaataaa aaatagtctt
     1321 cattttagaa gcacatactt tagcacctgt gtacttttga ttcaattcat aaacggtata
     1381 ccgctatagc atgtgtttta ttatttttct ggtctcttta tcgtaaatat tccccctaat
     1441 ttagcataat catttccggc taaacgagaa acagcctgta atgcgtgtgg attgatttta
     1501 aaatcaccct cataaatact gtcatcaata tgataagtta caatttcacc tattattaaa
     1561 tcagccccat ctaatacatc tccaagcaat atcatttgcg atagtttaca ttcgaatctc
     1621 attttcgcat ctttaattcc tggcgtctta atcgttgtag atgttaaaag tgataattct
     1681 gtacgactca actcactgtc accatatgct aacggcgctg cagtctcatt aatatcttga
     1741 acattatctt cgtctgtaat atgcacaaca aagtctccag tccgttctat atttaatgca
     1801 gtatcttttc tcttacctcc tgcacgttga actgcaatag caatcattgg cggatgatta
     1861 ttaacaatat taaaaaagct aaatggtgct gcatttactg atgcatcttg atttaatgtt
     1921 gtaacaaaag ctataggtcg tggaataatt gaaccaatta ataatttata gttttctcta
     1981 gcagttaatg attgtgcatc aaacgtatac ataataccta cctcttttct aagtatatct
     2041 aggtatttct ccgattttgg ttaatttaaa catctattct cctctgaaaa tcacttgtat
     2101 ttatttagca aatcttttga aatatgacac atatgcatat cttctggata tttttctaaa
     2161 tgttgctgat gttcttcagc acttttaatg tagttagaca gcggtaagac ttccactgca
     2221 atttgatctc tgtctttacg tcgttcaatg aactgacgcg ctgcaattaa gtggtcatct
     2281 acacaactat ataaacccgt tcgatacttt tgtccaatat catttccttg ttgattcaca
     2341 ctgtaaggat caatgatttc aaataaataa ttcataatgt ctgtaattgt taacatacga
     2401 tcatcgaaat gaagtttgac acattcagca taaccatcat acggaccgtc taatttagag
     2461 cttcttccat ttgctcttcc tgcttctgta tgtataattc caggtattgt tgcaaaaaat
     2521 gcttcaacac cccataaaca tcctcctgct acataaacaa ctgccatatt tacacctcat
     2581 catccttttt tatattttta acaaggttat accatttaat accgccatga catgattctg
     2641 atacaccttc attacgatac ccatattttt cataaaatga aattaatgat tctcgacatg
     2701 ttaacgttac accatgtcga tgatgattct tagcaagagt ttcaaaatag tttagtaagc
     2761 gacctgcaat accctgacct tgataatttg gtgctacaac aagacctaac acactaatat
     2821 agccaccttc actattattt gtggagacat ttttaaataa atcatcgcta atgtaacgct
     2881 cttttatgac tggaccgttg atgtatccaa ttacttcatc attcttttca gctacaataa
     2941 aagtttcttg aatttgttca attctcaatt tcaatgcctc tcgtgttgca gcttcttccg
     3001 gtgaaaaacc aatattttca agtgcaataa tttgatctaa gtcctgcatg attacttttc
     3061 ttaataccat aaaaacactt ccttagccct atatatcaaa agtaatacct tcatctttat
     3121 attcattatt ttcaaactac cttcactata aaaaacgagt ctttttattg aaagccattc
     3181 gccttaatca attagacaag ttgtattata cgaattagta aataatcatg attataattc
     3241 attttaagca tactcaaaaa gactggtaca tgtataccag tctaattcga aaaaatatat
     3301 ttagttaaaa ccatttcaaa aacgacttca cttttcctgg cttagtcggt ctgtctaaat
     3361 aaacaaaatt atcattgatt actgaactgg catttcgaag taatgcttca atatcattcg
     3421 aatatttctt caatttatga ttgtgaaata attcttgcat caaaaatggt ctttggtcac
     3481 atgaatgtgc atctgaagct acaaaatgag ccaaattaca ttctataaat tgtaatgata
     3541 acttttgaat gtttttacca aatccaccaa ctaaagaact cgatgttaat tgactcagtg
     3601 ccccatttgc aaccaattca tataatattt ccggattttt ggcgatactt ctatttcttt
     3661 caggatgtgc aatgattggt atgtaacctc tcgattgtat ttcaaaaaac aattgttttg
     3721 tataatgtgg tacttcgccc gttggaaatt caattaataa atatttcgaa cgattaatac
     3781 cttgaatact accattatct aagcctttca gaatcgaatc tgtaattcta atttcttgcc
     3841 cgggaaataa tttaatatcc aatgcttgaa cttctggatg cgttcttaac tccgccaatt
     3901 tcacaagcac ttgttgaaat gtattatcat atctcggatg caaatgatga ggtgtcgcta
     3961 caatacttgt tacaccttca tccttagctt gctttaatag tgcaatactc ttttcaattg
     4021 ttttaggacc atcatctata tcaactaata tatggttatg aatatcaatc atgattcatc
     4081 agtcccataa tatgcatagt aactagcact tttatcttta ggcattctat ttaagactac
     4141 acctaataat ttagcacctg ttgcttcaat aagttctttt ccttttttaa cttcatctct
     4201 attattattt tccgaattaa ctacgtagac aacattgccg gtaaactttg aaaataattg
     4261 cgcatctgta actgtgttca ctggtggcgt atcgataatt acaaagttat aattcatcaa
     4321 taatgtgtca tacaaatttg caaatgccct tgatgtaatt aactctgacg gattcggtgg
     4381 gattggccca gacgtcaaga cgtctaaatc ttgaatttca gttgagataa tactgtcttg
     4441 ataagttgac caatttagca ataaacttga taggccttca ttgtttggca aattaaaaat
     4501 ataatgctgc gtaggtttac gcatatcccc gtctacgatt agtgttttat aacctgcttg
     4561 cgcatatgca actgctaaat ttgctgcaat tgtagactta cctgcgcctg gtgcctctga
     4621 agtgattaca atgctttgaa ctgcactgtc aggatttgca aacataatat ttgatcttat
     4681 gcctcgaaat ttctcgctaa taggtgactt tggttgttca tggacaatta aacttgatgt
     4741 acttcttcgt gtattcgtca tggtaattcc tcgtaaatta aaatttttgt attgaaccta
     4801 aaataggtaa tcctagttgc gattcaacat cttcttctgt cttaatacgc ttatctaata
     4861 attcttttaa gaaaataatc aatattgcta aaacaatacc aacaataatg ctgataacta
     4921 agttgacaga tactattgga gatactttta cagcattatc atgtgctgag gaaagtatcg
     4981 taacattatc aacactcata attttaggca tgtcatgagc aaaaacttta gatattttat
     5041 taacaatttt gtcagattca gatttattcc cagtggtaac tgatacagta ataatttgag
     5101 agtttgtttg attggttact tttaaaaatg aattcaactc agctgttgaa tactgaccat
     5161 caaattctct agatacttta tctagaattc taggactttt gataatttcc gtatatgtat
     5221 taacagactg caaactactt tgaacatttt ggaaagctaa atcacttgag gactttttca
     5281 tgttcactaa tatttgagta gaagcagtat atttgtcagg cataacaaaa aaggttaatg
     5341 ccgcacttac tacaagacat attgccggta aaataagcaa taatttaata ttcttcttta
     5401 gaatatttaa tagttttact aaatcaaact tttct
//
LOCUS       DAXWFK010000159         5345 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.160, whole genome shotgun sequence.
ACCESSION   DAXWFK010000159 DAXWFK010000000
VERSION     DAXWFK010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5345
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.160"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            149..1816
                     /locus_tag="ACGEXS_002243"
     CDS             149..1816
                     /locus_tag="ACGEXS_002243"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFF8013231.1"
                     /translation="MTHLSDLDIANQSTLQPIKDIAASVGISEDALEPYGHYKAKIDI
                     NKITPRENKGKVVLVTAMSPTPAGEGKSTVTVGLADAFHELNKNVMVALREPALGPTF
                     GIKGGATGGGYAQVLPMEDINLHFNGDFHAITTANNALSAFIDNHIHQGNELGIDQRR
                     IEWKRVLDMNDRALRHVNVGLGGPTNGVPREDGFNITVASEIMAILCLSRSIKDLKDK
                     ISRITIGYTRDRKPVTVADLKVEGALAMILKDAIKPNLVQSIEGAPALVHGGPFANIA
                     HGCNSILATETARDLADIVVTEAGFGSDLGAEKFMDIKAREAGFDPAAVVVVATIRAL
                     KMHGGVAKDNLKEENVEAVKAGIVNLERHVNNIKKFGVEPVVAINAFIHDTDAEVEYV
                     KSWAKENNVRIALTEVWDKGGKGGVDLANEVLEVIDQPNSFKPLYELELPLEQKIEKI
                     VTEIYGGSKVTFSSKAQKQLKQFKENGWDNYPVCMAKTQYSFSDDQTLLGAPSGFEIT
                     IRELEAKTGAGFIVALTGAIMTMPGLPKKPAALNMDVTDDGHAIGLF"
     gene            complement(2061..2150)
                     /locus_tag="ACGEXS_002244"
     CDS             complement(2061..2150)
                     /locus_tag="ACGEXS_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013232.1"
                     /translation="MLLTTPIRLYLIDNNYQYQLLVLIVAHPN"
     gene            2149..4836
                     /gene="harA"
                     /locus_tag="ACGEXS_002245"
     CDS             2149..4836
                     /gene="harA"
                     /locus_tag="ACGEXS_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haptoglobin-binding heme uptake protein HarA"
                     /protein_id="HFF8013233.1"
                     /translation="MNKHHPKLRSFYSIRKSTLGVASVIVSTLFLITSQHQAQAAENT
                     NTSDKISENQNNNATTTQPPKDTNQTQPATQPANTAKNYPAADESLKDAIKDPALENK
                     EHDIGPREQVNFQLLDKNNETQYYHFFSIKDPADVYYTKKKAEVELDINTASTWKKFE
                     VYENNQKLPVRLVSYSPVPEDHAYIRFPVSDGTQELKIVSSTQIDDGEETNYDYTKLV
                     FAKPIYNDPSLVKSDTNDAVVTNDQSSSVASNQTNTNTSNQNTSTINNANNQPQATTN
                     MSQPAQPKSSTNADQASSQPAHETNSNGNTNDKTNESSNQSDVNQQYPPADESLQDAI
                     KNPAIIDKEHTADNWRPIDFQMKNDKGERQFYHYASTVEPATVIFTKTGPIIELGLKT
                     ASTWKKFEVYEGDKKLPVELVSYDSDKDYAYIRFPVSNGTREVKIVSSIEYGENIHED
                     YDYTLMVFAQPITNNPDDYVDEETYNLQKLLAPYHKAKTLERQVYELEKLQEKLPEKY
                     KAEYKKKLDQTRVELADQVKSAVTEFENVTPTNDQLTDLQEEHFVVFESEENSESVMD
                     GFVEHPFYTATLNGQKYVVMKTKDDSYWKDLIVEGKRVTTVSKDPKNNSRTLIFPYIP
                     DKAVYNAIVKVVVANIGYEGQYHVRIINQDINTKDDDTSQNNTSEPLNVQTGQEGKVA
                     DTDVAENSSTATNPKDASDKADVIEPESDVVKDADNNIDKDVQHDVDHLSDMSDNNHF
                     DKYDLKEMDTQIAKDTDRNVDKDADNSVGMSSNVDTDKDSNKNKDKVIQLNHIADKNN
                     HTGKAAKLDVVKQNYNNTDKVTDKKTTEHLPSDIHKTVDKTVKTKEKAGTPSKENKLS
                     QSKMLPKTGETTSSQSWWGLYALLGMLALFIPKFRKESK"
     gene            4967..5059
                     /locus_tag="ACGEXS_002246"
     CDS             4967..5059
                     /locus_tag="ACGEXS_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013234.1"
                     /translation="MKIILLLFLIFGFIVVVTLKSEHQLTLFSI"
     gene            complement(5039..>5345)
                     /locus_tag="ACGEXS_002247"
     CDS             complement(5039..>5345)
                     /locus_tag="ACGEXS_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFF8013235.1"
                     /translation="DYNGANISGIASASYSLFGIPPKDLSIAQSAYLIGLLQSPYGYT
                     PYEKDGTLKSDKDLKYSIQRQHYVLKRMLIEDQITEKEYNDALKYDIKSHLLNRKKR"
BASE COUNT         2037 a          807 c          984 g         1517 t
ORIGIN      
        1 agtgaattta ttgcaaaaat gaaagcgcta acccgattta gtcgacaagt ttttaacagt
       61 tcgttattat atgaatgtaa gtaaaaattt cttagctaca acttacatat tataaatgca
      121 taaattaaac aaaaaggggc gaaaaaagtt gactcattta tcagatttag atattgcgaa
      181 tcaatcaaca ctacaaccaa ttaaggatat tgctgcatca gtaggtattt cagaggatgc
      241 attagaacct tatggtcatt acaaagctaa aatcgacatt aataaaatta cgccaagaga
      301 aaacaaaggg aaagttgttt tagtaactgc gatgagccca acaccagctg gtgaaggtaa
      361 atcaacggtt acagttggtt tagctgatgc attccatgag ttaaataaaa acgttatggt
      421 tgcattaaga gagcctgctt taggaccaac atttggtatc aaaggtggtg cgactggtgg
      481 tggttatgcg caagtcttac ctatggaaga tatcaactta catttcaacg gagatttcca
      541 tgcgattaca actgcaaata atgcattgtc tgcgtttatc gataatcata ttcaccaagg
      601 taacgaatta ggaatcgatc aaagacgtat tgagtggaaa cgtgtattag atatgaatga
      661 tcgtgcactt agacatgtaa acgttgggtt aggtggacct acaaatggtg taccacgtga
      721 agatggcttt aatattacag tagcgtctga aattatggcg attttatgtt taagtagaag
      781 tattaaagac ttaaaagata aaattagtcg tattactatt ggttacacta gagatcgcaa
      841 gccagttaca gttgcagatt taaaagtgga aggtgcactt gcaatgattt taaaagatgc
      901 aataaaacca aacttagtac aatcaattga aggggcacct gcattagttc atggtggacc
      961 atttgcgaat atcgcacacg gttgtaactc aattttagca actgaaacag cacgtgattt
     1021 agctgatatc gttgtaacgg aagctggatt tggttcagac ttaggcgctg aaaaattcat
     1081 ggacattaaa gcgcgtgaag caggatttga tccggcagct gtcgttgttg ttgcgacaat
     1141 tcgtgcgtta aaaatgcatg gtggtgtagc gaaagataat ttaaaagaag aaaatgtaga
     1201 agcagtaaaa gcaggaattg ttaatttaga gcgtcatgtt aataatatta aaaaattcgg
     1261 tgtagaaccg gttgttgcaa ttaatgcatt tatacatgat accgatgcag aagtagaata
     1321 tgtaaaatct tgggctaaag aaaataacgt acgaattgcc ttaactgaag tttgggataa
     1381 aggtggtaaa ggtggcgttg acttagcaaa tgaagtatta gaagtcattg atcaacctaa
     1441 ttcatttaaa cctttatatg aattagaatt accattagag caaaagattg aaaagattgt
     1501 gactgaaatc tatggcggtt caaaagtaac gtttagcagt aaagcgcaaa aacaattaaa
     1561 acaatttaaa gaaaatggtt gggataatta cccagtatgt atggcgaaaa cacaatattc
     1621 attctcagat gatcaaacgt tgttaggtgc accatcagga tttgaaatta caattcgtga
     1681 attagaagcg aaaacaggtg caggatttat cgtagcgttg acaggtgcaa tcatgactat
     1741 gcctggttta cctaaaaaac cagcagcatt aaacatggat gttactgatg atggtcatgc
     1801 aattgggtta ttctaataaa tcatgtcaat tgtttaataa agataagtaa atagtttaat
     1861 agaccggact gttggagatg cattatttca gcagttcggt tttttgctgt gctaaaaata
     1921 gattcaattt ggcgaatcta acgacaatgt ttgaaggtgg ttaattaatg tatatgaaga
     1981 taaaaagtgg gcttgaagaa taggaaagcg atgcaatgaa tattccatat taaaaaaaat
     2041 aataaaatag gttgcaatat ttaattggga tgcgctacaa ttaacactaa taattgatat
     2101 tgataattat tatcaattaa atataatctt ataggagttg ttaacaacat gaacaaacat
     2161 cacccaaaat taaggtcttt ctattctatt agaaaatcaa ctctaggcgt tgcatcggtc
     2221 attgtcagta cactattttt aattacttct caacatcaag cacaagcagc agaaaataca
     2281 aatacttcag ataaaatctc ggaaaatcaa aataataatg caactacaac tcagccacct
     2341 aaggatacaa atcaaacaca acctgctacg caaccagcaa acactgcgaa aaactatcct
     2401 gcagcggatg aatcacttaa agatgcaatt aaagatcctg cattagaaaa taaagaacat
     2461 gatataggtc caagagaaca agtcaatttc cagttattag ataaaaacaa tgaaacgcag
     2521 tactatcact ttttcagcat caaagatcca gcagatgtgt attacactaa aaagaaagca
     2581 gaagttgaat tagacatcaa tactgcttca acatggaaga agtttgaagt ctatgaaaac
     2641 aatcaaaaat tgccagtgag acttgtatca tatagtcctg taccagaaga ccatgcctat
     2701 attcgattcc cagtttcaga tggcacacaa gaattgaaaa ttgtttcttc gactcaaatt
     2761 gatgatggag aagaaacaaa ttatgattat actaaattag tatttgctaa acctatttat
     2821 aacgatcctt cacttgtaaa atcagataca aatgatgcag tagtaacgaa tgatcaatca
     2881 agttcagtcg caagtaatca aacaaacacg aatacatcta atcaaaatac atcaacgatc
     2941 aacaatgcta ataatcaacc gcaggcaacg accaatatga gtcaacctgc acaaccaaaa
     3001 tcgtcaacga atgcagatca agcgtcaagc caaccagctc atgaaacaaa ttctaatggt
     3061 aatactaacg ataaaacgaa tgagtcaagt aatcagtcgg atgttaatca acagtatcca
     3121 ccagcagatg aatcactaca agatgcaatt aaaaacccgg ctatcatcga taaagaacat
     3181 acagctgata attggcgacc aattgatttt caaatgaaaa atgataaagg tgaaagacag
     3241 ttctatcatt atgctagtac tgttgaacca gcaactgtca tttttacaaa aacaggacca
     3301 ataattgaat taggtttaaa gacagcttca acatggaaga aatttgaagt ttatgaaggt
     3361 gacaaaaagt taccagtcga attagtatca tatgattctg ataaagatta tgcctatatt
     3421 cgtttcccag tatctaatgg tacgagagaa gttaaaattg tgtcatctat tgaatatggt
     3481 gagaacatcc atgaagacta tgattatacg ctaatggtct ttgcacagcc tattactaat
     3541 aacccagacg actatgtgga tgaagaaaca tacaatttac aaaaattatt agctccgtat
     3601 cacaaagcta aaacgttaga aagacaagtt tatgaattag aaaaattaca agagaaattg
     3661 ccagaaaaat ataaggcgga atataaaaag aaattagatc aaactagagt agagttagct
     3721 gatcaagtta aatcagcagt gacggaattt gaaaatgtta cacctacaaa tgatcaatta
     3781 acagatttac aagaagagca ttttgttgtt tttgaaagtg aagaaaatag tgagtcagtt
     3841 atggacggct ttgttgaaca tccattctat acagcaactt taaatggtca aaaatatgta
     3901 gtgatgaaaa caaaggatga cagttactgg aaagatttaa ttgtagaagg taaacgtgtc
     3961 actactgttt ctaaagatcc taaaaataat tctagaacgc tgattttccc atatatacct
     4021 gacaaagcag tttacaatgc gattgttaaa gtcgttgtgg caaacattgg ttatgaaggt
     4081 caatatcatg tcagaattat aaatcaggat atcaatacaa aagatgatga tacatcacaa
     4141 aataacacga gtgaaccgct aaatgtacaa acaggacaag aaggtaaggt tgctgataca
     4201 gatgtagctg aaaatagcag cactgcaaca aatcctaaag atgcgtctga taaagcagat
     4261 gtgatagaac cagagtctga cgtggttaaa gatgctgata ataatattga taaagatgtg
     4321 caacatgatg ttgatcattt atccgatatg tcggataata atcacttcga taaatatgat
     4381 ttaaaagaaa tggatactca aattgccaaa gatactgata gaaatgtgga taaagatgcc
     4441 gataatagcg ttggtatgtc atctaatgtc gatactgata aagactctaa taaaaataaa
     4501 gacaaagtca tacagctgaa tcatattgcc gataaaaata atcatactgg aaaagcagca
     4561 aagcttgacg tagtgaaaca aaattataat aatacagaca aagttactga caaaaaaaca
     4621 actgaacatc tgccgagtga tattcataaa actgtagata aaacagtgaa aacaaaagaa
     4681 aaagccggca caccatcgaa agaaaacaaa cttagtcaat ctaaaatgct accaaaaact
     4741 ggagaaacaa cttcaagcca atcatggtgg ggcttatatg cgttattagg tatgttagct
     4801 ttattcattc ctaaattcag aaaagaatct aaataattaa ctaaatatag catatgtatg
     4861 attaactttg tagacaatgt gaaagcaatt aatttataaa ctattgattg gtttaatggc
     4921 tttcctttag agtaaataaa aagaacagca gtgagaaatt ttctaattga aaataatctt
     4981 actgctgttt ttaatatttg gattcattgt tgtggttact ttaaaaagtg agcatcaatt
     5041 aacgcttttt tcgatttaac aaatgtgatt taatatcata ttttaatgcg tcgttgtatt
     5101 ctttttcagt gatttgatct tcgattaaca tacgctttaa tacataatgt tgtctttgaa
     5161 tactatattt caaatcttta tccgatttta acgttccatc tttttcgtag ggtgtatagc
     5221 catatgggct ttgcaacaaa ccgataaggt atgcagattg tgcaattgat aaatcttttg
     5281 gtggaatacc aaatagacta tatgaagcgg atgcaattcc ggaaatatta gcgccattat
     5341 aatct
//
LOCUS       DAXWFK010000160         5277 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.161, whole genome shotgun sequence.
ACCESSION   DAXWFK010000160 DAXWFK010000000
VERSION     DAXWFK010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5277
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.161"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            8..784
                     /locus_tag="ACGEXS_002248"
     CDS             8..784
                     /locus_tag="ACGEXS_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HFF8013236.1"
                     /translation="MMIHSKRLRLWLYLVLLAVFIGACGMKKEESSKDKQIKENFNKT
                     LSLYPTKNLEDFYDKEGFRDQEFEKGDKGTWIIHSKMTIETNGKNMESRGLVLYVDRN
                     TRTTKGEFIVRELWEDKKGYSRSKEKEYPVKMEHNKIIPTKPIADDKLRKEIENFKFF
                     VQYGDFKDINDYKDGDISYNPNVPSYSAKYQLSNDDYNVKQLRKRYDIPTKKAPKLLI
                     KGDGDLKGSSIGHKNLEFTFIENKEENIYFTDSINFKPTE"
     gene            896..1680
                     /locus_tag="ACGEXS_002249"
                     /pseudo
     CDS             896..1680
                     /locus_tag="ACGEXS_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501248.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
     gene            1677..3001
                     /locus_tag="ACGEXS_002250"
                     /pseudo
     CDS             1677..3001
                     /locus_tag="ACGEXS_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3001..3327
                     /locus_tag="ACGEXS_002251"
     CDS             3001..3327
                     /locus_tag="ACGEXS_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013237.1"
                     /translation="MFTTYKNINELENAYDEERKQLNDAFNQIDELRHQTRKKCEQMY
                     DHFLYLKHKMNYSEDAMIRMTRIIESFDRETNQRIRHHEMKLEDYKDELRREYLKQSD
                     RIEGDE"
     gene            3330..3617
                     /locus_tag="ACGEXS_002252"
     CDS             3330..3617
                     /locus_tag="ACGEXS_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013238.1"
                     /translation="MGLDFSGLPDLAVLEQMKEKEQISEVIAPEHVRMHHDHQNKLKS
                     DEKILLDQMVSHFKKFEDDFKNAAQGAWVKNATDELKDISNDLEKIQDIKV"
     gene            3934..4041
                     /locus_tag="ACGEXS_002253"
                     /pseudo
     CDS             3934..4041
                     /locus_tag="ACGEXS_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014533130.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Csa1 family protein"
     gene            4153..4947
                     /locus_tag="ACGEXS_002254"
     CDS             4153..4947
                     /locus_tag="ACGEXS_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HFF8013239.1"
                     /translation="MIHSKKLTLGICLVLLIILIVGYVIMTKTNGRNAQIKDTFNQTL
                     KLYPTKNLDDFYDKEGFRDQEFKKGDKGTWIVNSEMVIEPKGKDMETRGMVLYINRNT
                     RTTKGYYFISEMTDDSNGRPKDDEKRYPVKMEHNKIIPTKPLPNDKLKKEIENFKFFV
                     QYGNFKDINDYKDGDISYNPNVPSYSAKYQLNNDDYNVQQLRKRYDIPTKQAPKLLLK
                     GDGDLKGSSVGSRSLEFTFVENKEENIYFTDSVQYTPSEDTRYESN"
     gene            complement(4951..5073)
                     /locus_tag="ACGEXS_002255"
     CDS             complement(4951..5073)
                     /locus_tag="ACGEXS_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013240.1"
                     /translation="MSYIIKFTFKFFEMATYISQLTLDIELVSSELLVILPGFI"
BASE COUNT         2170 a          669 c          962 g         1476 t
ORIGIN      
        1 atgtgaaatg atgattcact caaaaaggtt gaggctctgg ctgtatttag tattattagc
       61 tgtttttata ggggcttgcg gaatgaaaaa ggaagaaagc agtaaagata aacaaattaa
      121 agaaaacttc aacaaaacgt tgagcctgta tccaactaaa aatcttgaag acttttatga
      181 taaggaaggc tttcgagatc aagagtttga aaagggcgac aaaggcactt ggataattca
      241 ctctaaaatg acaatagaaa caaatggaaa gaatatggaa tccagaggat tggtcctgta
      301 tgtagaccgc aataccagaa cgacaaaagg tgaatttatt gtgagggaat tatgggaaga
      361 taaaaaagga tactcacgta gtaaagaaaa agaatatcca gtaaaaatgg aacataataa
      421 aattatccca accaaaccga tagctgatga taaattaaga aaagaaatcg aaaactttaa
      481 attctttgta caatacggtg actttaaaga tattaatgat tataaagatg gtgacatttc
      541 atataatccg aatgtgccaa gttattcagc aaagtatcaa ttaagtaatg atgactacaa
      601 tgtgaagcaa cttagaaaga ggtatgatat accaactaag aaagcaccta aattattaat
      661 aaaaggcgat ggtgatttaa aaggatcatc tataggacac aaaaatttag agtttacatt
      721 tatagaaaat aaagaagaaa acatctattt tacggatagt attaatttca aacctactga
      781 atagggttaa tagaagtttg aaatgaaaat ttcataaagt gttcaaaata atttttcgct
      841 ttagttgtgt aaattaaaga ttgagtcagt attaaaaata taaggtggag aaatgatgat
      901 tcattcaaag aaattaacgc tcggtatatg tttggtatta ctcattatat tgattggagg
      961 ttgtgtcatt atgacaaaaa caaatggtcg aaacgctcaa attaaagaaa atttcaacaa
     1021 aacattaagt gtatatccaa ccaagaatct cgatgatttt tacgataagg aaggttttcg
     1081 agatcaagaa tttgataaaa gagataaagg gacttggatt atttattctg aaatggttat
     1141 cgaaccaaaa gggaaaaata tggaatcgag aggaatggtg ctctatatca atcgcaatac
     1201 tagaacaacg aagggtaatt ttattgtcac cgaaataact gaagatagta aaggatattc
     1261 acgtagtaaa gaaaaaaata tcctgtcaag atggaaaata atcgaattat tccaacaaag
     1321 cctataccgg atgacaagtt aaaaaaagag attgaaaact ttaagttctt tgtacaatat
     1381 ggaaatttta aagattttaa agattataaa aatggtgata tttcatataa tcctaatgtg
     1441 ccaagttatt ctgcaaagta tcaattgaat aatgatgact ataatgttca acagttaaga
     1501 aaacgatatc atattccaac caaacaagcg cccgaattaa aattgaaagg atccggcaat
     1561 ttaaaaggct catccgtagg atctaaggat ctagaattta cgtttgtaga aaatcaagaa
     1621 gagaatatct atttttcaga ttcggtcgaa tttacaccta gcgaggatga taaatcatga
     1681 gtcaaacgga atatcaaata aaatctggca atataaaagg taactctgaa gaaacaagta
     1741 cagtatctaa tataagttat gaaatagaaa acgcaaataa cagtggttta aaacaaaata
     1801 aaattgataa acaaattaaa aagttacaag aaaaaaataa attccctaaa aatctttcat
     1861 atcttaaaag ttatacggac cccaaaacag gcacgactac aagcgccttt ttaaataaag
     1921 acactggcaa agttacttta ggtatgacag gtactaatgt acacaaagac gcaatattaa
     1981 aacaaacatt tggtgttcct tcttatcaag gatatataga tgtgagtgaa acgctaaagg
     2041 atattggggc cgatgtcaat attggccttc attccgtcac agataaagat ccacattata
     2101 aaaataccca agactttatc aaaaatatca aaaaagacta tgatattgat attattaccg
     2161 gacattcgct gggcggtaga gatgcgatga ttttaggtat gagtaatgat attaaacata
     2221 tcgttgtgta taatccagct ccattagcga tcaaagatgt gagcggcctt tatgccgatc
     2281 aagaagaact taaaaaattg attgaaaagt acgatggtca cattgtaaga tttgtgtctg
     2341 atgaagacga attagatgca ggtgtccgca atcatttata tgaaactgct ggagaaaaaa
     2401 tagtacttaa aaatggagaa ggccatgcaa tgagtggtat tttaatgagc agaacacagg
     2461 ctataatctt agctgaatta aacaaagtta aaggctacca agacgaaaat aataaagcat
     2521 taaaatccgt tcgtaaacaa acgaggcata gattacataa agtagagacg ttaagagcga
     2581 attggattca aacaacgggt ggatcactct cttcctccca acaacaatta ttagaagctt
     2641 taacagcact aaccattgcc gaaggcttaa atcaattagt gaatgaagaa agccaacatt
     2701 tgaaaaaatg tatcacgcga tggcacataa atttggagac aactggaaaa aagcgcaaga
     2761 agttggaaat gaaattggtg aaaaattaac ctctgaagag gttatagatg aattaagaaa
     2821 aggtggcgcg tatgaaagta aacttgaaac agatcccaaa agaaaaattg atgataagat
     2881 aaagaaatta aatgatgttt ataaaaattg taatggctat atcgcaaaaa ttaaacagag
     2941 tatcgaagca attgtttcta atgaccaaat gttagcgagc cagattgatg ggatgatgta
     3001 atgtttacta cgtataaaaa tattaatgaa cttgaaaatg cctatgatga agaaagaaaa
     3061 caattgaatg atgcattcaa tcaaattgat gaattaagac atcaaacacg caagaaatgt
     3121 gaacaaatgt atgatcattt cttatatctc aaacataaaa tgaattatag tgaagacgct
     3181 atgatcagga tgacacgtat tatagaatct ttcgatagag aaacgaatca acgtatccga
     3241 catcacgaaa tgaaattaga agattataaa gatgagttaa gaagagaata tctaaaacaa
     3301 tctgacagaa ttgaaggaga tgaataagcg tgggacttga ttttagtggt ttaccagatt
     3361 tagcagtatt ggaacaaatg aaggaaaaag aacagattag tgaggttatt gcgcctgaac
     3421 atgttcgtat gcatcatgat catcaaaata agctgaaaag tgatgagaaa atattacttg
     3481 accaaatggt aagtcatttc aaaaaatttg aagatgattt taaaaatgcg gcacaagggg
     3541 cttgggtgaa aaatgccaca gacgaattaa aagatattag taatgattta gaaaaaattc
     3601 aagatattaa agtataaaaa ggtattaaga aacacaataa gtatataatc catttaataa
     3661 taaatgaatt atatagttca taatttcgac tataagtggc tattagccat tatttttcgg
     3721 gatctatgtc aaatcggact aatgaattca ataatggaag ttaagcaacc aatctttgtt
     3781 taacttcttt tttatttttt ggaaaataaa gttttgaaca taataaaatt tgattatgtt
     3841 ttaacgaatt ttgatgtttc ttgaactata tatcatctag tcgtcattta caattggtaa
     3901 atatgacttc aaactgtatg aaggtgatgg cgattaaaag gctcatccgt aggttctaaa
     3961 gaactagaat ttaactttat aagaaataaa gaagaaaatg tgtatttttc tgatagtatt
     4021 aatttcaaac ctactgaata gggttaatag aagtttgaaa taaaaatttc ataaagtgtt
     4081 caaaataatt tttcgcttta gttgtaaata ttaaaaactg agtcaatatt aaaaatataa
     4141 ggtggagaaa tgatgattca ttcaaagaaa ttaacgctcg gtatatgctt ggtattactc
     4201 attatattga ttgtaggtta tgtcattatg acaaaaacaa atggtcgaaa cgcccaaatt
     4261 aaagacacat ttaatcaaac attaaaatta tatccaacca aaaatctcga tgatttttac
     4321 gataaagaag gctttcgaga tcaagaattt aaaaagggtg ataaaggtac ttggatagtt
     4381 aattctgaaa tggtaatcga gccaaaaggt aaggatatgg aaacgagagg aatggtgctc
     4441 tatatcaatc gcaatactag aaccacaaaa gggtattatt ttataagtga aatgacagat
     4501 gacagtaacg gcagaccaaa ggatgatgaa aaaaggtatc cggtaaaaat ggaacataat
     4561 aaaatcatac caacgaagcc actaccgaat gacaagttaa aaaaagagat tgaaaacttt
     4621 aagttctttg tacaatatgg caactttaaa gatattaatg attataaaga tggtgatatt
     4681 tcatataatc ctaatgtacc aagttattcg gcaaaatatc aattgaataa tgatgattat
     4741 aatgtccaac agttaagaaa aagatatgat attccaacca aacaagcgcc gaaactatta
     4801 ttgaaaggcg atggagattt aaaaggttca tccgtaggtt ctagaagtct tgaatttacc
     4861 tttgtcgaaa ataaagaaga aaacatttat tttacagatt ctgtacaata tactccaagt
     4921 gaggatacaa ggtatgagtc aaactgaata tcaaataaaa cctggtaata taacaagtaa
     4981 ctctgaagaa acaagttcga tatctaaagt gagctgtgaa atataggtag ccatttcaaa
     5041 aaatttaaag gtgaatttga taatgtagct caaggagatt gggttaaaaa ggcgaagaat
     5101 gaactggatg atattagtaa gaaattaaaa aatattcaaa gaacggaagt ttaatagctt
     5161 atatgattct tggagctaag acagcatgcg ttcattcatg ccattattaa tataagcacc
     5221 gcaacaaaaa agcttctaat gtgatacagg aaccttatat tccgtatcat gttagaa
//
LOCUS       DAXWFK010000161         5189 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.162, whole genome shotgun sequence.
ACCESSION   DAXWFK010000161 DAXWFK010000000
VERSION     DAXWFK010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.162"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(44..691)
                     /locus_tag="ACGEXS_002256"
     CDS             complement(44..691)
                     /locus_tag="ACGEXS_002256"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFF8013241.1"
                     /translation="MKFDNYIFDFDGTLADTKKCGEVATQSAFKACGLTEPSSKEITH
                     YMGIPIEESFLKLADRPLDEAALAKLIDTFRHTYQSIEKDYIYEFAGITEAITSLYNQ
                     GKKLFVVSSKKSDVLERNLSAIGLNHLITEAVGSDQVSAYKPNPEGIHTIVQRYNLNS
                     QQTVYIGDSTFDVEMAQRAGMQSAAVTWGAHDARSLLHSNPDFIINDPSEINTVL"
     gene            complement(804..1352)
                     /gene="hxlB"
                     /locus_tag="ACGEXS_002257"
     CDS             complement(804..1352)
                     /gene="hxlB"
                     /locus_tag="ACGEXS_002257"
                     /EC_number="5.3.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0043800 - hexulose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HFF8013242.1"
                     /translation="MAKFSDYQLMLDELKMTLSHVEADEFSTFASKILHAEHIFVAGK
                     GRSGFVANSFAMRLNQLGKQAHVVGESTTPAIKSNDVFVIISGSGSTEHLRLLADKAK
                     SVGADIVLITTNKDSAIGNLAGTNIVLPAGTKYDEQGSAQPLGSLFEQASQLFLDSVV
                     MGLMTEMNVTEQTMQQNHANLE"
     gene            complement(1354..1986)
                     /gene="hxlA"
                     /locus_tag="ACGEXS_002258"
     CDS             complement(1354..1986)
                     /gene="hxlA"
                     /locus_tag="ACGEXS_002258"
                     /EC_number="4.1.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043801 - hexulose-6-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019647 - formaldehyde assimilation via
                     ribulose monophosphate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hexulose-6-phosphate synthase"
                     /protein_id="HFF8013243.1"
                     /translation="MELQLAIDLLNKEDAAELANKVKDYVDIVEIGTPIIYNEGLPAV
                     KHMADNISNVKVLADMKIMDAADYEVSQAIKFGADVITILGVAEDASIKAAIEEAHKN
                     NKQLLVDMIAVQDLEKRAKELDEMGADYIAVHTGYDLQAEGQSPLETLRTVKSVIKNS
                     KVAVAGGIKPDTIKDIVAESPDLVIVGGGIANADDPVEAAKQCRAAIEGK"
     gene            complement(2063..2821)
                     /gene="nagB"
                     /locus_tag="ACGEXS_002259"
     CDS             complement(2063..2821)
                     /gene="nagB"
                     /locus_tag="ACGEXS_002259"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFF8013244.1"
                     /translation="MKVLNLGSKKQASFYVACELYKEMAFNQHCKLGLATGGTMTDLY
                     EQLVKLLNKNQLNVDNVSTFNLDEYVGLTASHPQSYHYYMDDMLFKQYPYFNRKNIHI
                     PNGDADDMNAEASKYNDVLEQQGQRDIQILGIGENGHIGFNEPGTPFDSVTHIVDLTE
                     STIKANSRYFKNEDDVPKQAISMGLANILQAKRIILLAFGEKKRAAITHLLNQEISVD
                     VPATLLHKHPNVEIYLDDEACPKNVAKIHVDEMD"
     gene            3097..3459
                     /locus_tag="ACGEXS_002260"
     CDS             3097..3459
                     /locus_tag="ACGEXS_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YojF family protein"
                     /protein_id="HFF8013245.1"
                     /translation="MLEPIKEQEVIDLLASFEHKPVYLHVETTNGAYANHFDQRVFNA
                     GTFLRNIQITYTHAQLKGGNKEPYRIGLKLSNGGWVYVQGLTHFEVNEHDEFLIAGFN
                     YEGQLAAALQISERPFNL"
     gene            3473..4138
                     /gene="bshB2"
                     /locus_tag="ACGEXS_002261"
     CDS             3473..4138
                     /gene="bshB2"
                     /locus_tag="ACGEXS_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB2"
                     /protein_id="HFF8013246.1"
                     /translation="MTDERHVLVIFPHPDDETFSSAGTLANYIQKGIPVTYACLTLGQ
                     MGRNLGNPPFATRESLPSIRERELEEACKVIGITDLRKMGLRDKTVEFEPYEHIDGMI
                     KSLIDDTNPSLIISFYPGYAVHPDHEATADAVIRTVERMPKEERPRLTLVAFSNDATE
                     ALGEPDIQNDITDFKELKIKAFEAHASQTGPFLKQLASPEIDGKQHSFLTVEPYWTYH
                     FKS"
     gene            4151..5029
                     /gene="folE2"
                     /locus_tag="ACGEXS_002262"
     CDS             4151..5029
                     /gene="folE2"
                     /locus_tag="ACGEXS_002262"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HFF8013247.1"
                     /translation="MTEFDLSTREGRWKHFGSVDPIEGTKPTTKNEMTDLQSTHKDFL
                     FEIEEVGIKNLVYPVLVDQYQTAGTFSFSTSLTKDEKGINMSRIIESVEKHYDNGIEL
                     EFNTLYQVLRTLQTNMKQNAAGVDVSGKWFFDRYSPTTNIKAVGNADVTYGLAIDGDK
                     VTRKELTIEATVTTLCPCSKEISEYSAHNQRGVVTVKTYINKDQDIVDDYKNKILDAM
                     EANASSILYPILKRPDEKRVTERAYENPRFVEDLIRLIAADLVEFDWLDGFDIECRNE
                     ESIHQHDAFAKLKYRK"
BASE COUNT         1669 a          940 c          822 g         1758 t
ORIGIN      
        1 tgaatatgca ttttaaccat ggtattctct gttttaacaa gttttataat acggtattaa
       61 tttctgatgg atcattaata ataaaatccg gatttgaatg aagtaatgac cttgcatcat
      121 gtgcacccca agtgacagct gcagattgca taccagcacg ttgtgccatc tcaacatcaa
      181 acgttgaatc accaatatac accgtttgtt ggctatttaa attgtagcgt tgcacaattg
      241 tgtgtatgcc ttcaggattt ggtttatatg cacttacttg atcggatcca acagcttcgg
      301 taatcaagtg atttaatcca atagccgata aatttctttc taatacatca ctcttcttac
      361 tagacaccac gaaaagtttt ttcccttggt tatacaaact tgtaatggct tcagttatac
      421 ccgcaaattc ataaatatag tccttttcaa tagattgata tgtatgtcta aatgtatcga
      481 ttaactttgc taatgctgct tcatctaatg gtcggtctgc taattttaaa aatgattctt
      541 caataggtat tcccatataa tgcgttattt ctttagatga tggttccgtt aagccacatg
      601 ctttaaatgc actttgtgtt gctacttcac cacatttttt cgtgtctgcc aacgtaccat
      661 caaaatcaaa aatataattg tcaaacttca atattaacac ccctattcgt ttattattga
      721 tattatacta taatttatta tttaaataaa aaaataatcg ataatatttc tgcctaggca
      781 tcatattatc gactatcttt attttattct aaattagcat gattttgttg catcgtttgt
      841 tccgtaacat tcatttcagt catcaatccc attacaacac tatctaaaaa taattgagat
      901 gcttgttcaa acaaacttcc taatggttgt gccgagcctt gttcatcata ttttgtacct
      961 gcaggcaaaa cgatgttcgt cccagctaga ttgcctattg cagaatcttt atttgtagta
     1021 attaatacga tgtcagcacc tactgatttt gctttgtctg ctaataatct taaatgttcc
     1081 gtggaacctg aaccagagat aattacaaat acatcattcg acttaatcgc aggtgtcgtt
     1141 gattctccaa caacatgtgc ctgtttgccg agctgattta agcgcattgc aaaactattc
     1201 gccacgaatc ctgaacgtcc tttgccagct acaaatatat gttcagcatg tagtattttg
     1261 gatgcaaaag ttgaaaactc atccgcttca acatgtgaca aagtcatctt taattcatct
     1321 agcattaatt gatagtcact aaatttagcc atattactta ccttcgattg cagcgcgaca
     1381 ttgttttgca gcttctactg gatcatctgc atttgcgatt ccgccaccaa caataacaag
     1441 atcaggactt tcagcgacaa tatctttaat tgtatctggt ttaattccac ctgctactgc
     1501 aactttagaa tttttaataa cagatttaac ggttcttaaa gtttctaatg gtgattgccc
     1561 ttctgcttgt aaatcataac cagtgtgtac tgcaatataa tcagcaccca tttcatctag
     1621 ttcttttgca cgtttttcta aatcttgaac agcaatcata tcaactagta attgtttatt
     1681 atttttatga gcttcttcaa tagctgcttt aattgatgca tcttctgcaa cacctagtat
     1741 tgtaattaca tccgcgccaa atttaattgc ttggctaact tcataatcag ctgcatccat
     1801 aattttcatg tctgctaata cttttacatt actaatgttg tctgccatat gtttaactgc
     1861 tggtaaacct tcgttgtaaa tgattggcgt accgatttct acgatatcta cataatcttt
     1921 tactttattt gctaactcag ccgcgtcttc tttgtttaat aaatcaattg ctaattgtaa
     1981 ttccactaaa aatcatcctt ccaatgcttt ttatctttct attggaacct ttcccgaggc
     2041 atttcttaat taaacattgc aatcaatcca tttcatcgac atgaattttc gcaacatttt
     2101 tcgggcaagc ttcgtcgtct aaatatatct caacattcgg gtgtttgtga agtaatgtgg
     2161 ctggaacatc aacagaaatt tcctgattta ataaatgtgt aatagcagca cgtttctttt
     2221 caccaaatgc gagtaaaatg atacgtttgg cttgaagaat attagcaagt cccatcgaaa
     2281 tggcttgctt tggaacatca tcttcgtttt taaaatatcg actattagcc ttaatagtac
     2341 tttcagtcaa atcaacgata tgagtaacgc tatcaaacgg cgtaccaggt tcattaaatc
     2401 caatatgacc attttcacca atacctaaaa tttgaatatc acgttgacct tgttgttcta
     2461 aaacgtcatt atattttgac gcttccgcat tcatatcatc ggcatctcca tttggaatat
     2521 gaatgttctt tctattaaaa taaggatatt gtttgaaaag catgtcatcc atataatagt
     2581 gataactttg cggatgtgat gcggttaaac ctacatattc gtctaaatta aacgtggata
     2641 cattgtctac gtttaactga tttttattta ataacttaac aagttgttca tacaaatctg
     2701 tcattgtacc accagttgct aaacctagtt tacagtgctg attaaatgcc atctctttat
     2761 ataactcaca tgcaacatag aatgatgctt gttttttcga tcctaagttt aatactttca
     2821 ttgtttacat gcctccacaa atttaataca ttttaaaaac ttatgaattc attcatacac
     2881 cattaatata gcatcatttt taatgtttaa cacttttcaa attccaacaa atttttgatt
     2941 tttaaaagta aaaaactaat gctgtatgat tacgtaatcc tgagtaatta cccttcatga
     3001 aaattaattt ataaataatt gagcaatcgt atacaattat cgaatagttt ttagtaaaat
     3061 agaaagataa acacattgat agtgtaagga gtgaacgtgt tggaaccaat taaagaacaa
     3121 gaagttatag atttacttgc ttcttttgaa cataaacctg tgtatctgca tgttgaaacg
     3181 acaaatgggg cctatgcgaa ccatttcgat caacgtgttt ttaatgctgg tactttttta
     3241 agaaacatac aaataacata tacgcatgca caactaaaag gtggtaataa agaaccttat
     3301 cgcattggtc ttaaactatc aaatggtggt tgggtttatg tacaaggtct aacgcatttt
     3361 gaggtcaatg agcatgatga atttttaatt gcaggtttta attacgaagg tcaactcgct
     3421 gcagcacttc aaattagtga gcgaccattt aatttataga ggaggcatca acatgactga
     3481 cgaaagacat gtattagtaa tatttccaca tcctgatgat gaaaccttct catctgcagg
     3541 cacattagca aattatatcc aaaaaggcat acctgtaaca tatgcatgtt taaccctagg
     3601 acaaatgggc agaaatttag gtaatccccc atttgcaaca cgcgaatcat taccaagtat
     3661 tcgtgagcgt gaactagaag aagcttgtaa agttatcggt attactgatt taagaaaaat
     3721 gggtttacgt gacaaaactg tcgaatttga accctatgaa catatagatg gtatgattaa
     3781 atctttaatc gatgatacaa atccatcatt gattatttca ttttatcctg gatatgcagt
     3841 acatcctgat cacgaagcaa cagcagacgc tgtaattcga acggttgaac gcatgcctaa
     3901 agaagaacgc cctcgtttaa ctttagttgc gttcagtaat gatgcaaccg aagctcttgg
     3961 agaacctgat attcaaaatg atattacaga ttttaaagaa cttaaaatta aggcatttga
     4021 agcacatgct tctcaaacag gtccattttt aaaacaatta gcaagtcctg aaatcgatgg
     4081 taaacaacac agctttttaa cagtcgaacc atattggaca tatcacttta aatcttaacg
     4141 gaggaaaaaa atgactgaat ttgatttatc gacacgagaa ggtcgttgga aacactttgg
     4201 ttccgtagat cctattgaag gcacgaaacc tactactaag aacgaaatga cagatttaca
     4261 aagtacacat aaagattttt tatttgaaat agaagaagtt ggtattaaaa acttagtgta
     4321 ccctgtactt gttgaccagt atcaaacagc tggcacattt agtttttcaa caagtttaac
     4381 aaaagacgaa aaaggtatta atatgagccg tataatcgaa agtgttgaaa agcactatga
     4441 taatggtatt gaacttgaat ttaatacact ttatcaggtg ttacgcactt tacaaacgaa
     4501 tatgaaacaa aatgcggcag gcgttgatgt ttctgggaaa tggttctttg atcgctatag
     4561 tccaaccaca aatattaaag cagttggtaa tgccgatgtc acttatggat tagcaattga
     4621 cggtgataaa gttacacgta aagaacttac tattgaagca actgtcacaa cgctatgccc
     4681 ttgttcaaaa gaaataagtg agtattcagc gcacaaccaa cgtggtgttg tcactgttaa
     4741 aacatatatt aataaagatc aagacatagt tgatgactat aaaaataaaa ttttagatgc
     4801 aatggaagct aatgcaagtt caattctata ccctatttta aaacgtcctg atgaaaaacg
     4861 tgtgacagag cgtgcatatg aaaatccacg ttttgtcgaa gacttaattc gtttaattgc
     4921 cgcggactta gttgaatttg attggttaga cggtttcgat atcgaatgtc gtaatgaaga
     4981 gtctatccat caacatgatg ctttcgctaa attaaagtat cgcaaataat atcatatgat
     5041 taattcaaac gcgtaacatt agttgcgcgt ttttattata ccttctagag ttgtatatct
     5101 cttttataaa tcttctcatt tgatgtgcct gagtcataaa tcaatatcct aagctctaca
     5161 atgatatatt ggtcgtagtt gattgaata
//
LOCUS       DAXWFK010000162         5173 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.163, whole genome shotgun sequence.
ACCESSION   DAXWFK010000162 DAXWFK010000000
VERSION     DAXWFK010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5173
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.163"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            18..2006
                     /gene="tkt"
                     /locus_tag="ACGEXS_002263"
     CDS             18..2006
                     /gene="tkt"
                     /locus_tag="ACGEXS_002263"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001661967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFF8013248.1"
                     /translation="MFNEKDQLAVDTLRALSIDTIEKANSGHPGLPMGAAPMAYTLWT
                     RHLNFNPQSKDYFNRDRFVLSAGHGSALLYSLLHVSGSLELEELKQFRQWGSKTPGHP
                     EYRHTDGVEVTTGPLGQGFAMSVGLALAEDHLAGKFNKEGYNVVDHYTYVLASDGDLM
                     EGISHEAASFAGHNKLSKLVVLYDSNDISLDGELNKAFSENTKARFEAYGWNYLLVKD
                     GNDLEEIDKAITTAKSQEGPTIIEVKTTIGFGSPNKAGTNGVHGAPLGEVERKLTFEN
                     YGLDPEKRFNVSEEVYEIFQNTMLKRANEDESQWNSLLEKYAETYPELAEEFKLAISG
                     KLPKNYKDELPRFELGHNGASRADSGTVIQAISKTVPSFFGGSADLAGSNKSNVNDAT
                     DYSSETPEGKNVWFGVREFAMGAAVNGMAAHGGLHPYGATFFVFSDYLKPALRLSSIM
                     GLNATFIFTHDSIAVGEDGPTHEPIEQLAGLRAIPNMNVIRPADGNETRVAWEVALES
                     ESTPTSLVLTRQNLPVLDVPEDVVEEGVRKGAYTVYGSEETPEFLLLASGSEVSLAVE
                     AAKDLEKQGKSVRVVSMPNWNAFEQQSEEYKESVIPSSVTKRVAIEMASPLGWHKYVG
                     TAGKVIAIDGFGASAPGDLVVEKYGFTKENILNQVMSL"
     gene            2284..2526
                     /locus_tag="ACGEXS_002264"
     CDS             2284..2526
                     /locus_tag="ACGEXS_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFF8013249.1"
                     /translation="MATWLAIIFIVAALILGLIGGFLLARKYMMDYLKKNPPINEEML
                     RMMMMQMGQKPSQKKINQMMTMMNKNMDQNMKSAKK"
     gene            2513..2611
                     /locus_tag="ACGEXS_002265"
     CDS             2513..2611
                     /locus_tag="ACGEXS_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013250.1"
                     /translation="MRKSKFAIDRGYFPDMEMASFYNQINKNKYVY"
     gene            2705..3172
                     /locus_tag="ACGEXS_002266"
     CDS             2705..3172
                     /locus_tag="ACGEXS_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdC family protein"
                     /protein_id="HFF8013251.1"
                     /translation="MLYLIFSIIVALFMGTIVIVIRMKAQNYPVNEKKIVLPPFFMAT
                     GALMYVVPYFRLTGSEMLEAFIIGLLFSTVLIWTSRFEVKGTEIYMKRSKAFPVILIS
                     LLIIRTVMKIFISNEIDPGELGGMFFLLAFCMIVPWRAAMLYKYKKLKKTLIN"
     gene            3296..4417
                     /gene="sbcD"
                     /locus_tag="ACGEXS_002267"
     CDS             3296..4417
                     /gene="sbcD"
                     /locus_tag="ACGEXS_002267"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFF8013252.1"
                     /translation="MKIIHTADWHLGKILNGKQLLEDQAYILDMFVEKMKEEEPDIIV
                     IAGDLYDTTYPSKDAIMLLEQAIGKLNLELRIPIIIISGNHDGKERLNYGASWFEHNQ
                     LFIRTDFTSINSPIEINGVNFYTLPYATVSEMKHYFEDDTIETHQQGITRCIETIAPE
                     IDEDAVNILISHLTVQGGKTSDSERPLTIGTVESVQKGVFDIFDYVMLGHLHHPFSIE
                     DDKIKYSGSLLQYSFSEAGQAKGYRRVTINDGIINDVFIPLKPLRQLEIISGEYNDVI
                     NEKVHVKNKDNYLHFKLKNMSHITDPMMSLKQIYPNTLALTNETFNYNEENNAIEISE
                     KDDMSIIEMFYKHITDKELSDIQSKKIKNILENELRKED"
     gene            4421..>5173
                     /locus_tag="ACGEXS_002268"
     CDS             4421..>5173
                     /locus_tag="ACGEXS_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8013253.1"
                     /translation="MKPLHLKLNNFGPFLKEEIDFSKIDNNELFLISGKTGSGKTMIF
                     DAMTYALFGKASTEQREENDLRSHFADGKQPMSVTFEFQLNHRIYKVHRQGPYIKEGN
                     TTKTNAKFDVFEMVDGKYEIRESKVISGTQFIIELLGVNADQFRQLFILPQGEFKRFL
                     ISNSREKQGILRTLFDSEKFEAIREILKEEVKKEKAQIENRYQQIDLLWQEIESFDDD
                     NIKGLLEVATQQIDKLIENIPLLQARSKEILAS"
BASE COUNT         1889 a          697 c          978 g         1609 t
ORIGIN      
        1 aagggaagat acacaagatg tttaatgaaa aagatcaatt agctgttgat acgctacgtg
       61 cactaagtat cgacacaatc gaaaaagcga attctggtca tccaggatta cctatgggag
      121 ctgccccaat ggcttacact ttgtggacac gtcatctgaa ttttaatcca caatctaaag
      181 attacttcaa tagagaccgt ttcgtattat ctgcagggca tggttcagca ttattgtata
      241 gcttgttaca tgtttctggt agtttagaat tagaagaatt aaagcaattt agacaatggg
      301 gttctaaaac accaggtcat cctgaataca gacatacaga tggtgtagaa gttactaccg
      361 gaccacttgg acaaggtttt gctatgtcag taggattagc tttagcagaa gatcacctag
      421 cagggaaatt taataaagaa ggatataatg ttgtagatca ttacacatat gtattagctt
      481 ctgacggtga tttaatggaa ggtatatcgc atgaagcagc ttcatttgct ggacataata
      541 aattaagtaa attagttgtt ttatacgatt caaatgatat ttcattagat ggcgaattaa
      601 acaaagcttt ttctgaaaac acaaaagctc gttttgaagc atatggttgg aattacttac
      661 tagttaaaga tggtaatgat ttagaagaaa ttgataaagc gattactaca gctaaatctc
      721 aagaaggacc aacgattatt gaagttaaaa caacaatcgg atttggttca ccgaataaag
      781 caggaactaa tggtgttcat ggggcacctt taggtgaagt tgaaagaaaa ttaacattcg
      841 aaaattacgg tttagatcct gaaaaacgtt ttaatgtttc agaagaggta tacgaaattt
      901 tccaaaatac tatgttaaaa cgtgctaatg aagatgaatc tcaatggaat tcattattag
      961 aaaaatatgc agaaacatat cctgaattag cagaagaatt taaattagcg attagtggta
     1021 aattgcctaa aaattataag gatgaattac cacgttttga actgggtcat aatggtgcat
     1081 ctcgtgctga ttctggtact gttattcaag caatcagtaa aactgtccct tcattctttg
     1141 gtggatcagc agaccttgct ggttcaaaca aatccaatgt aaatgatgca actgattata
     1201 gttctgaaac acctgaaggt aaaaatgtgt ggtttggtgt acgtgaattt gctatgggtg
     1261 ctgctgtaaa tggtatggct gcacatggag gtttacatcc atatggtgca acattcttcg
     1321 tatttagtga ttatttaaaa ccagcgttac gtttatcatc aattatggga ttaaatgcaa
     1381 cgttcatctt cacacatgat tcaattgcag taggtgaaga tggtcctact catgaaccaa
     1441 ttgagcaatt agctggatta agagccattc caaatatgaa tgttatccgt cctgctgatg
     1501 gtaatgaaac aagagtagca tgggaagttg ccttagaatc tgaatctaca cctacttcat
     1561 tagtattgac acgtcaaaac ttaccggtat tagatgtacc agaagatgta gttgaagaag
     1621 gcgttcgaaa aggtgcctat acagtttatg gctctgaaga gacaccagaa ttcctattat
     1681 tagcttcagg ttcagaagtt agtcttgcag ttgaagctgc taaagatctt gaaaaacaag
     1741 gtaaatcagt acgtgttgtt tcaatgccta actggaatgc atttgaacaa caatctgaag
     1801 aatataaaga atcagttatt ccatcaagcg taacaaaacg tgttgcgatt gaaatggctt
     1861 caccgcttgg atggcataaa tatgtaggta ctgcaggtaa agttattgct attgacggct
     1921 ttggcgcaag tgcacctggc gatttagtag ttgaaaaata tggatttaca aaagaaaata
     1981 tcttaaacca agttatgagc ttataagaat aatttataaa gcgagtatgt ttagaagtct
     2041 aggatgcata atcttaggct tcttttaaag tgttgaaatt tagagtatag cacttaaact
     2101 acatcataag tgataagtta tgaaagtata ctatttcaga ttaatcttta aaagctctgt
     2161 tataacagca tgatttttga tattattttt agtatcgata ttaaaatact tgaataaact
     2221 agttcttgaa ataatgtgat gaatttagta aaattcagta ggataaagaa agcgggtgaa
     2281 acaatggcaa cttggttagc aattattttt atagtagctg cattaatttt aggtttaatt
     2341 ggaggtttcc ttttagctag aaaatatatg atggactact tgaagaaaaa cccaccaatc
     2401 aacgaagaaa tgcttcgtat gatgatgatg caaatgggtc aaaaaccttc tcagaagaaa
     2461 attaatcaaa tgatgacgat gatgaataaa aatatggatc aaaatatgaa gagtgcgaaa
     2521 aagtaaattc gcaattgata gaggctattt tccagatatg gaaatggcct ctttttataa
     2581 tcaaattaat aagaataaat atgtttatta aaattaaagt taacaaaatg acgaatagac
     2641 tgagaaatgc tataattcat tttgtatgat ttacagagag tttatttaac gagaaggtgt
     2701 ctgcgtgctc tatttaatat tttcaatcat tgtagcttta tttatgggaa ctatagttat
     2761 agttattcgt atgaaagctc aaaattatcc ggtaaatgag aaaaaaatag ttttgccacc
     2821 gttttttatg gcgaccggtg cattgatgta cgtcgttcca tattttaggc taacaggatc
     2881 ggaaatgcta gaagccttta taattggttt gcttttttct acagttctaa tttggacttc
     2941 tcgatttgaa gtcaaaggta cagaaattta tatgaaacga tctaaagcat ttccagttat
     3001 tttgatttca ttacttatca ttcgtactgt gatgaaaata ttcattagta atgaaataga
     3061 tcctggagaa ttaggcggca tgttcttttt attagcattc tgtatgattg ttccttggag
     3121 agcagcaatg ctatataaat acaaaaaact aaagaaaaca ttaatcaatt aattactttt
     3181 aaaaccactt gtgatcgact tctaaatcag tcaatgagtg gttttaattt tacttgaaaa
     3241 ggtgaaatga ttatattaaa ataaaagcga acaaatgttc tatatggagg cagtaatgaa
     3301 aattatacat acagcagact ggcacttagg gaaaatatta aatggcaaac agcttttaga
     3361 agatcaagcg tatattttag atatgttcgt agaaaaaatg aaagaagaag aacctgatat
     3421 cattgtgata gctggagatt tatatgacac aacatatcca agtaaagatg caatcatgtt
     3481 attagaacaa gcgattggaa agttaaattt agaactgcgt ataccaataa ttattattag
     3541 tggaaatcac gatggtaaag agaggttaaa ctatggggcg agttggtttg aacataatca
     3601 gttatttata agaacagatt ttacatcgat taattcacca atagagataa atggggttaa
     3661 tttttataca ctcccttatg ctactgtgag cgaaatgaaa cactactttg aagatgacac
     3721 cattgaaaca catcaacagg gaattacgcg ctgtattgaa acaatagcac cggaaattga
     3781 tgaagatgcc gtcaatattt taattagtca tctgactgtt caaggtggaa agacatctga
     3841 ttctgaaaga ccattaacta ttggaacggt tgaatcagtt cagaaaggtg tttttgatat
     3901 atttgattat gtcatgctag gtcacttgca tcatccattt agtatagaag acgacaaaat
     3961 taaatatagt ggctccttat tgcagtattc attttcggaa gcgggtcaag ctaaagggta
     4021 tagacgtgta acaattaatg atggcattat taacgatgta tttattcctc ttaagccact
     4081 tagacaattg gaaattatct caggcgaata taatgatgtt attaatgaaa aagttcatgt
     4141 gaaaaataaa gataattatt tacattttaa acttaaaaat atgtctcata ttactgatcc
     4201 aatgatgagt ttaaaacaaa tttatcctaa tactttagcg ctgacgaatg aaacttttaa
     4261 ttacaatgaa gaaaataatg ctatagaaat aagtgaaaaa gatgacatgt caattatcga
     4321 aatgttttat aaacatataa ctgataaaga attatcggat atccaatcta aaaagataaa
     4381 aaatatttta gaaaacgaat tgagaaagga ggattaatga atgaaaccat tacatttaaa
     4441 gttgaataat ttcggcccct ttttaaaaga agaaattgat ttttctaaaa ttgataataa
     4501 tgaattgttt ttaataagtg gtaagactgg atcgggtaaa acaatgattt ttgatgcaat
     4561 gacttatgcc ttgtttggta aagcatcaac tgaacaaaga gaagaaaatg atttgagaag
     4621 tcatttcgct gatggtaaac agccgatgtc agtaacattt gaatttcaat taaatcatcg
     4681 aatttataaa gtgcatagac aaggccctta tatcaaagaa ggtaatacaa caaaaacgaa
     4741 cgctaaattt gatgtatttg agatggtgga tggcaagtat gaaattagag aaagtaaagt
     4801 aatttcaggt acccaattca ttattgaatt attaggagta aatgcagatc aattccgaca
     4861 attgtttatt ttgcctcaag gtgaattcaa acgcttttta atatcaaaca gtcgtgaaaa
     4921 gcaagggata ttaagaacac tgtttgacag tgaaaaattt gaagctatac gagaaatatt
     4981 aaaagaagaa gtaaaaaaag aaaaagctca aatcgagaat agatatcaac aaattgacct
     5041 tttatggcaa gaaattgaat catttgatga tgacaatata aaaggcttat tagaggttgc
     5101 cactcaacag atagacaaat tgattgaaaa tataccactt ttacaagcta ggtcgaaaga
     5161 aatactagca tct
//
LOCUS       DAXWFK010000163         5166 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.164, whole genome shotgun sequence.
ACCESSION   DAXWFK010000163 DAXWFK010000000
VERSION     DAXWFK010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5166
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.164"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            235..1332
                     /locus_tag="ACGEXS_002269"
     CDS             235..1332
                     /locus_tag="ACGEXS_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HFF8013254.1"
                     /translation="MYINMKDYGLTGINKTKDTRAIQRALNRGRCKPTTVYIPKGTYD
                     ICKPLTIYGNTTLLLDNETILRRCHSGPLLKNGRRFGFYRGYNGHSHIHIKGGKFDMN
                     GVSYPYNNTAMCIGHAEDIQLIGVTIKNVVSGHAIDACGINGLYIKSCSFEGFIDYSG
                     ERFYSEAIQLDIQVPGAFPKFGTTDGTITKNVIIEDCYFGPSELPEMGSWNRAIGSHA
                     SRHNRYYENIHIRNNIFEDIQGYALTPLKYKDAFIINNKFINCEGGIRYLGVRDGKNA
                     ADVMTGKDLGSQAGINMNIIGNEFKGSMSKDAIHVRNYNNVKHKDVLIVGNTFNNSTQ
                     SIHLEDIDTVFLSPVEAGIQVTTINVDEIKK"
     gene            1537..3099
                     /locus_tag="ACGEXS_002270"
     CDS             1537..3099
                     /locus_tag="ACGEXS_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFF8013255.1"
                     /translation="MAYFNQHQSMISKRYLTFFSKSKKKKPFSAGQLIGLILGPLLFL
                     LTLLFFHPQDLPWKGVYVLAITLWIATWWITEAIPIAATSLLPIVLLPLGHILTPEQV
                     SSEYGNDIIFLFLGGFILAIAMERWNLHTRVALTIINLIGASTSKILLGFMVATGFLS
                     MFVSNTAAVMIMIPIGLAIIKEAHDLQEANTNQTSIQKFEKSLVLAIGYAGTIGGLGT
                     LIGTPPLIILKGQYMQHFGHEISFAKWMIVGIPTVIVLLGITWLYLRYVAFRHDLKYL
                     PGGQTLIKQKLDELGKMKYEEKVVQTIFVLASLLWITREFLLKKWEVTSSVADGTIAI
                     FISILLFIIPAKNTEKHRRIIDWEVAKELPWGVLILFGGGLALAKGISESGLAKWLGE
                     QLKSLNGVSPILIVIVITIFVLFLTEVTSNTATATMILPILATLSVAVGVHPLLLMAP
                     AAMAANCAYMLPVGTPPNAIIFGSGKISIKQMASVGFWVNLISAIIIILVVYYVMPIV
                     LGIDINQPLPLK"
     gene            complement(3110..3217)
                     /locus_tag="ACGEXS_002271"
     CDS             complement(3110..3217)
                     /locus_tag="ACGEXS_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013256.1"
                     /translation="MVDFLLFVLFLHDAAVYVSLPLVQAIANVTFYFSF"
     gene            complement(3289..4083)
                     /locus_tag="ACGEXS_002272"
     CDS             complement(3289..4083)
                     /locus_tag="ACGEXS_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFF8013257.1"
                     /translation="MQLYYLGPKGTFSYLACRQYFSENEATFQPKSNLFEVIKAVADD
                     DTSIGVVPIENSIEGTINIVADALAQQDVFAHGEIRLDINFALYGNGTDSIPDIKKVY
                     SIAPAISQTTNYIHQHQFDYDYVDSTIQSLTKIENGVAAIAPLGSGEAYGFTPIDTHI
                     EDYPHNVTRFLVIKNQQQFDQNATSLMFLITPMHDKPGLLASVLNTFALFNINLSWIE
                     SRPLKTQLGMYRFFVQADSAITTDIKKVIAILETLDFKVEMIGAFN"
     gene            complement(4103..>5166)
                     /locus_tag="ACGEXS_002273"
     CDS             complement(4103..>5166)
                     /locus_tag="ACGEXS_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitric oxide synthase oxygenase"
                     /protein_id="HFF8013258.1"
                     /translation="AQAFIENMYKECHYETQIINKRLHDIELEIKETGTYTHTEEELI
                     YGAKMAWRNSNRCIGRLFWDSLNVIDARDVTDEASFLSSITYHITQATNEGKLKPYIT
                     IYAPKDGPKIFNNQLIRYAGYDNCGDPAEKEVTRLANHLGWKGKGTNFDVLPLIYQLP
                     NESVKFYEYPTSLIKEVPIEHNHYPKLRKLNLKWYAVPIISNMDLKIGGIVYPTAPFN
                     GWYMVTEIGVRNFIDDYRYNLLEKVADAFEFDTLKNNSFNKDRALVELNYAVYHSFKK
                     EGVSIVDHLTAAKQFELFERNEAQQGRQVTGKWSWLAPPLSPTLTSNYHHGYDNTVKD
                     PNFFYKKKESNANQCPFHH"
BASE COUNT         1731 a          771 c          939 g         1725 t
ORIGIN      
        1 cgcaatcaac aaataatata agaagttttt gcgatagttt taaaatagct gtaatagaat
       61 actaaatgtg acaaacttag aactaatatc aagtgttgat gttttgaata taaaaatgct
      121 aatagatggc atcacgagat ttatgactat ctgttaaatc aacctaacac gattataatt
      181 tgaataattg gttaatatat gagtaattag aaaatagaca aaggatgacg atttatgtat
      241 atcaatatga aagattatgg gttaacaggc ataaacaaaa ctaaagatac tcgagcaata
      301 caacgtgcgt taaatcgtgg aagatgtaaa ccaacgacag tttatatacc gaaagggacg
      361 tatgatattt gcaaaccatt aacgatatat ggcaatacaa cacttttgtt agataatgaa
      421 actattttac gccgatgtca ttctggtcct ttattaaaaa atggtcgtcg ctttggtttt
      481 tatcgtggtt ataatggaca cagtcatatt catattaaag gcggcaagtt tgatatgaat
      541 ggtgtatcgt atccttataa caatacagct atgtgcattg ggcatgctga agatattcaa
      601 ttaataggtg tgaccattaa gaatgtagtg agtggtcatg caattgatgc ttgtgggatt
      661 aacggactct atattaaaag ctgttcattt gaaggattca tagactatag tggcgaacgc
      721 ttttattctg aagcaataca attagacatt caagtacctg gtgcttttcc aaaattcgga
      781 acgacagatg gtacgataac gaaaaatgtc attatcgaag attgttattt tggaccttca
      841 gaattgcccg aaatgggaag ttggaatcgt gctattggct cacatgcaag tagacataat
      901 cgatactatg agaatattca tattagaaat aatatatttg aagatataca aggttatgca
      961 ttaactccct tgaagtataa agatgctttc attattaata ataagtttat taactgtgag
     1021 ggtggcatta gatatttagg agttagagat ggtaaaaatg cagcagatgt gatgacagga
     1081 aaagacttag gttcccaagc aggcataaat atgaatataa ttggaaatga atttaaagga
     1141 tcaatgtcta aagatgcgat acatgtacgt aattataata atgttaaaca taaagatgta
     1201 ttaatcgttg ggaatacatt caataattcg actcaatcaa ttcatttaga agatattgat
     1261 acagtgtttt taagtcctgt tgaagcgggt attcaagtta ctacaatcaa tgtagatgaa
     1321 ataaaaaagt aaaaagtttc gcatgacatt aggattaaga atagtagata atttttgaaa
     1381 gcgcattcat aaaacggtat aaatatgcta taataaaccc aattatctga taaaaggggt
     1441 attttgacgg taatgataat acaagataga caactttcta tactctaata tagtgagttg
     1501 aagtagcttg tcataatcat catgaggggg aaatttatgg cttatttcaa tcaacatcaa
     1561 tcaatgatat cgaaaaggta tttaacattc ttttcaaaat caaagaaaaa gaaaccgttt
     1621 agtgcgggac aacttattgg actaatatta ggtccattac ttttcctatt aacattatta
     1681 ttctttcatc cacaagactt accttggaaa ggcgtctatg ttttagcgat tactttatgg
     1741 attgcgactt ggtggattac tgaagcaatt cctattgcag caacgagctt attaccaatt
     1801 gtgttattac cattaggtca tatacttaca ccagaacaag tatcatccga atatggcaat
     1861 gatattatct ttttgttttt aggtggattt attttggcaa ttgcaatgga aagatggaat
     1921 ttacatacga gagtagcgtt gacaattatt aatttgattg gtgcaagcac ttcaaaaata
     1981 ttacttggat tcatggtggc aacaggattc ttatctatgt ttgtatcgaa cactgcagct
     2041 gtaatgatta tgattccgat tggtttagca attattaagg aagcacatga tttacaagaa
     2101 gccaatacga atcaaacaag tattcaaaag tttgaaaaat ctctagtttt agcaattggc
     2161 tatgcaggta cgattggtgg cttgggtaca ttaatcggaa ccccgccatt aattatttta
     2221 aaaggacaat acatgcaaca ttttggacat gaaattagtt ttgctaaatg gatgattgta
     2281 gggattccaa cggtcattgt tttgttaggt attacttggc tctatttaag atatgttgcg
     2341 tttagacatg atttgaaata tttacctggt ggtcagacgt taattaaaca aaagttagac
     2401 gagcttggca aaatgaagta tgaagaaaag gtagtacaaa ctatctttgt acttgctagc
     2461 ttattatgga ttacaagaga gtttcttctg aaaaaatggg aagttacgtc atctgttgca
     2521 gatggtacga ttgctatttt tatatcaata ttattattta ttattccagc taaaaatact
     2581 gaaaaacatc gccgtatcat tgactgggaa gttgcaaaag agctcccttg gggtgtatta
     2641 attttatttg gtggcggttt agcattagcg aaaggtattt ctgaaagtgg tttagcaaaa
     2701 tggttaggcg aacagttgaa atcattaaat ggtgttagtc cgattcttat tgtaattgtc
     2761 ataacaatct ttgtcttatt tttaactgaa gtgacatcta atactgcaac tgcaacgatg
     2821 attttaccga ttttagcaac gttgtctgtt gctgttggag tgcatccatt actacttatg
     2881 gcacctgcag ctatggcggc taactgtgca tacatgttac cagtagggac accaccgaat
     2941 gcaattatct ttggttctgg taaaatatct atcaaacaaa tggcatcagt aggattctgg
     3001 gtaaacttaa tcagtgcaat aattattatt ttagtcgtgt attatgtaat gcctatagtt
     3061 ttaggtattg atataaatca accactgcca ttgaaatagt aattgcagat tagaacgaaa
     3121 aataaaaggt tacattagca attgcttgga cgagtggtaa cgaaacgtat accgcagcat
     3181 cgtgtaaaaa caatacaaac aaaagaaagt caaccaagga tggattccta ttttaatcct
     3241 tggttgactc tttattttat ttaaattgta gaacctagaa aataaagttt aattaaaagc
     3301 accaatcatt tctactttga aatctaaggt ttctaaaata gcaatgactt tctttatatc
     3361 ggttgtaatt gcagaatcag cctgaacgaa aaatcgatac atacctaatt gtgtttttaa
     3421 aggacgagac tcaatccagg ataaattaat attaaacaaa gcaaatgtat taagcacact
     3481 tgctaacaac ccaggtttat catgcattgg tgtaattaaa aacatcaatg atgtcgcatt
     3541 ttgatcaaat tgctgctgat tttttataac taaaaaacgt gtcacgttat gtggatagtc
     3601 ttcaatatgt gtatcaatag gtgtaaaacc ataagcttcg ccactaccta aaggtgcaat
     3661 tgctgcaacg ccattttcaa ttttagtcaa actttgaatt gtactgtcga cataatcata
     3721 gtcaaattga tgttgatgta tgtaatttgt tgtttgactg attgctggtg caatagaata
     3781 cactttttta atatcaggaa tggaatctgt tccattacca tataatgcaa agttaatatc
     3841 taaacgtatt tcaccgtgtg caaagacatc ttgctgtgca agtgcatctg ccacaatgtt
     3901 gattgttcct tctatagaat tttcaatagg gacaacacca atcgatgtgt catcatctgc
     3961 aactgccttg atgacttcaa ataaatttga ctttggttga aaagttgctt cattttcaga
     4021 aaaatactga cgacaagcca aatatgaaaa tgtaccttta gggcctaaat aatataattg
     4081 catatgctac acctctacta acttaatgat ggaaagggca ctggttagca tttgattctt
     4141 tctttttata gaaaaagttt ggatctttta ctgtattgtc atatccgtga tgataatttg
     4201 acgtcaatgt tggagataat ggcggtgcta gccaagacca ttttccggta acttgacgac
     4261 cttgttgtgc ttcgttacgt tcgaatagtt cgaattgctt tgcagcggtc aaatgatcga
     4321 caattgatac gccttctttt ttaaaggaat gatacacagc atagttcaat tcaacaagtg
     4381 ctcgatcttt attaaatgaa ttatttttaa gtgtatcaaa ttcaaacgca tctgcaactt
     4441 tttctagtaa attgtaacgg taatcatcaa taaagttacg tacgccaatt tcagttacca
     4501 tataccaacc gttaaagggt gcagttggat atacaatgcc accgattttt aagtccatat
     4561 tggaaatgat agggactgca taccatttta agttcaattt tcttaatttt ggataatgat
     4621 tatgttcaat aggtacttct ttaattaatg aagtaggata ttcgtaaaat ttaactgact
     4681 cattaggtaa ttggtaaatc agtggtaaca cgtcaaaatt agtacctttt cctttccaac
     4741 ctaagtgatt tgctaagcgt gtaacttctt tttcagcagg atcaccacaa ttgtcatagc
     4801 cagcatagcg aattaattga ttgttgaaaa ttttaggtcc atcctttgga gcatatatag
     4861 taatatacgg ctttaattta ccttcatttg tagcctgtgt aatatgataa gtaattgatg
     4921 ataagaacga tgcttcgtca gtaacatctc ttgcatcaat gacatttaac gaatcccaaa
     4981 ataaacgacc aatgcaacga tttgaattac gccaagccat tttagcacca taaataagtt
     5041 cttcttctgt atgtgtatat gtcccagttt cttttatttc tagttcaatg tcatgtaaac
     5101 gtttattgat aatttgcgtt tcataatgac actctttata catgttttct atgaaagctt
     5161 gagcct
//
LOCUS       DAXWFK010000164         5164 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.165, whole genome shotgun sequence.
ACCESSION   DAXWFK010000164 DAXWFK010000000
VERSION     DAXWFK010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.165"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            212..835
                     /locus_tag="ACGEXS_002274"
     CDS             212..835
                     /locus_tag="ACGEXS_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8013259.1"
                     /translation="MKEDRRIRKTKSSIKQAFTKLLQEKDLEKITIRDITTRADINRG
                     TFYLHYEDKYMLLADMEDEYISELTTYTQFDLLRGSSIEDIANTFVNNILKNIFQHIH
                     DNLEFYHTILQLERTSQLELKINEHIKNNMQRYISINHSIGGVPEMYFYSYVSGATIS
                     IIKYWVMDKQPISVDELAKHVHNIIFNGPLRIMAENRLHKSNLDSLT"
     gene            881..1825
                     /locus_tag="ACGEXS_002275"
     CDS             881..1825
                     /locus_tag="ACGEXS_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFF8013260.1"
                     /translation="MITSFRHSEDIDKHIIKTPLDHTASWINVVEPDREEIENLMEQY
                     NIPEDFIRDPLDSEESSRIEYDEDTGYSLIIIDLPIVNSTNRSVLSFVTIPLGIIIGN
                     GIIVTVCDAENEFLENLPKRDINLKFHSRFALEILTTIADHYNRNLRLLNKSRIRIEK
                     ELKNNITNKQLFKLMEVEKSLVYFLAALKGNDTIIKKLFRLPAIKRFEEDEELLEDLI
                     IENNQAIETTELHQRILESITTSYASLLSNDMNTIMKTLTLFTVLLTLPTLVFSFFGM
                     NVPLPIDDHSYISWIIVVGISLILVVIVSIFLWRKQKL"
     gene            complement(1911..3353)
                     /locus_tag="ACGEXS_002276"
     CDS             complement(1911..3353)
                     /locus_tag="ACGEXS_002276"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFF8013261.1"
                     /translation="MNYKQSAEEILNAIGGEENLDAMAHCATRLRLVLNDESLVNEEA
                     LNNMDVVKGTFSTGGQYQIIIGSGTVNKVFSELEKLTGKEASTTSEVKAQSAKNMNPL
                     QRFVKMLSDIFVPIIPAIVAGGLLMGLNNILTAKDLFFSGKSLIDVYSQFAGLAEMIN
                     VFANAPFTLLPILIGFSAAKRFGGNPFLGAALGMILVHPSLMSAYDFPKAVEAGKAIP
                     YWDVFGLHINQVGYQGQVLPMLVAAYILASIEKGLRKVIPTVLDNLLTPLLSIFITAF
                     LTFSFVGPITRQLGYWLSDGLTWLYEFGGAIGGLIFGLLYAPIVITGMHHSFIAVETT
                     LIADATKTGGSFIFPIATMSNVAQGGAAIAAFFIIKQNKKLKGVASAAGISALLGITE
                     PAMFGVNLKLRYPFIGAIVGSGIGSAYIAFFKVKAIALGTAGLPGFISINPVHAGWLH
                     YFVGMTISFIIAITVTLILSKRKANKEVVE"
     gene            3536..3919
                     /locus_tag="ACGEXS_002277"
     CDS             3536..3919
                     /locus_tag="ACGEXS_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFF8013262.1"
                     /translation="MKERGYIEQLWREEKYHVLLHSQQSYQMIRNALKTDLSLHQVQQ
                     MIDVALLIEPSIGSVCNAFDHMWGYLKKCANEEERQQSKLLKADFINGKIDTQTLLDF
                     LAELANKYDVQYLLQSRVLNTKRKR"
BASE COUNT         1758 a          814 c          783 g         1809 t
ORIGIN      
        1 catagttgct attggtgcaa tccataataa tttattttta aatatattca ttttattaat
       61 tacctcctta ttagacaatg tgttgaatat ttcgcatgtt gtctattata atatgtaagt
      121 gggaataaac aatacacaat gttcatcaat acgttcatta atagacaaat acacccacat
      181 tgttcaataa cacaaggagg ttgatttatt tttgaaagaa gataggcgaa ttagaaagac
      241 aaagtcctcc attaaacaag cgttcactaa attactacaa gaaaaagatt tagaaaagat
      301 taccattcgc gacataacaa cacgcgctga tatcaataga gggacatttt acttacatta
      361 cgaggataaa tatatgttac tcgcagatat ggaagatgag tatatttcag aactaacgac
      421 atatactcaa tttgatttgt tacgcggttc ttcaattgaa gacattgcga atacttttgt
      481 aaataatata ctcaaaaata ttttccaaca tattcatgat aatttagagt tttatcatac
      541 tattttacaa ttggaacgca cgagtcaatt agaattgaaa atcaacgaac atattaaaaa
      601 taatatgcaa cgttacatta gtatcaatca ttctatcgga ggcgttccag agatgtattt
      661 ttacagttac gtttccggag caacaatttc aattattaaa tactgggtaa tggacaaaca
      721 gcccatttca gttgatgaat tagctaaaca tgtgcataat attattttta atggtccatt
      781 aagaataatg gcagaaaacc gtttgcataa atcaaacctt gattcactaa cttaagtaat
      841 actcgctttt cctaaatcct ttagaaagga actatatatc atgattactt cattcagaca
      901 ttctgaagat atagataaac atattataaa aacaccatta gatcatacag cgtcatggat
      961 taatgtagta gaaccagacc gagaagaaat tgaaaatctt atggaacaat ataatatacc
     1021 tgaagacttt atacgtgacc ctttggactc agaagaaagt tcccgtattg aatatgacga
     1081 agatactggt tactcattaa tcattattga tttacctatc gtcaattcaa ctaatcgtag
     1141 cgttctatct tttgtaacga ttccattagg cattattatt ggcaacggta ttatcgtaac
     1201 agtttgtgac gctgaaaatg aatttttaga aaatttacct aagcgtgata ttaatttaaa
     1261 atttcacagc agatttgcat tagaaatttt aactactatt gccgaccatt ataatcgtaa
     1321 cttacgatta cttaataaaa gtagaattcg tattgagaaa gaactaaaaa ataacattac
     1381 taacaaacaa cttttcaaat taatggaagt tgaaaaaagt ttagtatact ttttagctgc
     1441 cttaaaaggt aacgatacaa ttattaaaaa gttattccgt ttacctgcaa ttaaacgttt
     1501 tgaagaagat gaagaattac tagaagattt aatcattgaa aataaccaag ccatcgaaac
     1561 aacagaatta catcaacgta tcttagaaag tatcacaaca tcatacgctt ccttattatc
     1621 taatgatatg aatacgatta tgaagacatt aacacttttc acggtactat taacgttacc
     1681 aacactcgta tttagtttct tcggtatgaa tgtgccgttg ccaattgatg accatagtta
     1741 tatatcttgg attattgtgg taggtatttc attaattctt gtcgtaattg ttagtatttt
     1801 cttatggcgt aaacagaaat tataactaaa ttaaaaggta tgacagctta aagtttcatc
     1861 ctattgcatt tgcaatgact gatactttga ttactgtcat acctcttttt ttattctaca
     1921 acttctttat ttgcttttct tttagataaa attaaagtaa ctgttatagc aatgatgaat
     1981 gatattgtca taccaacaaa gtagtgtaac catcctgcat gtactggatt gattgaaata
     2041 aatcctggca atccagcagt tcctaatgcg attgctttaa ccttgaagaa agcaatatat
     2101 gctgaaccaa tacctgatcc aacgatagcg ccaataaatg gatatcttag ttttaagtta
     2161 acaccaaaca tagccggttc tgtaatacca agtaatgctg aaatacctgc ggcagatgcc
     2221 acacctttta acttcttatt ttgtttaata ataaagaacg ctgcaattgc tgcaccacct
     2281 tgtgcaacat tagacattgt cgcaatcggg aatataaatg atccacccgt tttagtggca
     2341 tctgcaatta atgtcgtttc tacagctata aagctatgat gcatacctgt aataacaatc
     2401 ggagcataca ataatccgaa tattaatcca ccaattgcac caccaaattc ataaagccat
     2461 gttaaaccat ctgataacca gtaacctaat tgtcgagtga ttggacctac aaatgaaaat
     2521 gttagaaatg ctgttataaa aatagataac aatggtgtta acaaattatc taacaccgtt
     2581 ggaataactt tgcgtaaccc tttttcaatt gaggctaaga tataagctgc tacaagcata
     2641 ggtaacactt gtccttgata acctacttga ttaatatgca aaccaaaaac atcccaatat
     2701 ggaatagcct tacctgcttc aactgctttt gggaaatcgt atgcgctcat tagcgatgga
     2761 tgaactagta tcatacctaa tgcagcacct aaaaatggat tgccaccaaa gcgttttgct
     2821 gcactaaatc caattaaaat tggtaataat gtaaatggtg cattcgcaaa aacatttatc
     2881 atttcagcta atccagcaaa ttgactatat acatctatca atgatttacc tgaaaagaat
     2941 aaatctttcg cagtcaaaat gttatttaac cccattaata aaccaccagc aacgatggct
     3001 ggtataatcg gaacaaagat atctgaaagc atttttacaa atcgctgtaa cggattcata
     3061 tttttagcag attgtgcttt gacttccgaa gtggttgatg cttcttttcc agttaatttt
     3121 tccagttcac taaatacttt attgactgta ccagacccaa taataatttg gtattgtccc
     3181 ccagtagaaa acgtcccttt aactacatcc atattgttta gcgcctcttc atttactaaa
     3241 ctttcatcat ttaaaactaa tcgtagtctc gttgcacaat gcgccattgc atctaaattc
     3301 tcttctccgc ctatcgcgtt caaaatttct tctgcggatt gcttataatt catatattta
     3361 gcctcctaat tagaaccggt tccatttatt ccactttatt gtaaccggtt ccaaaaatat
     3421 ttgcaacact ctattatcat tttatataaa cacctacacc atttttgata gcgcattcat
     3481 ttttattgct atactacatg catgtatatt aacattaatt atcggaggta attttatgaa
     3541 agaacgtggc tatatcgaac agctttggcg tgaggaaaaa tatcatgtat tgttacacag
     3601 tcaacagtct taccaaatga taaggaatgc tttaaaaacg gatttatcac ttcatcaagt
     3661 tcaacaaatg attgatgttg ctttgttaat tgaaccgagt ataggaagtg tatgtaatgc
     3721 ttttgatcat atgtggggat atttaaaaaa atgtgcgaat gaagaagaaa gacaacaatc
     3781 aaaactactt aaagctgatt ttattaatgg taaaatcgac acccaaactt tattagattt
     3841 tttagcagaa cttgctaata aatatgatgt tcaatattta ctacaaagtc gtgttttaaa
     3901 cacaaaaaga aagagataga ttgaaacttt agttgtaacg gacaaatagt agtcattgga
     3961 gttcagcaat tcatactttg tactaaagtt tactataatc tatctctcaa ggtttaaatg
     4021 tacatatcta tattaaaatg cgtttcatga tgttagaaga taccatacac atttaattag
     4081 ttacagcata aatcaattta tacccttaat tattacattg tttatatttt aaattcgggg
     4141 gagtaatttt aagtaatatc ttgttgctgc taattctatt gttaataaat tgatattaga
     4201 tgtagaaatt gagtgtgaaa gttaataata gatgatttag ctagtaacat aattaattca
     4261 tatatagtca aaaagttctg ttagatactg ttcttgatga atattctttg atgttaggtg
     4321 ttagctattc tagatgagtt tcttagatgt ggatgcctgt cttgatgaag ttatctatga
     4381 tgttaatata atacttgatg ttgattctta gatgttagtg cgatttcaag atgctgattc
     4441 tttgttgttg cactatcttg gtgacgtttc tagttgttta tagttatcgc atctcttgat
     4501 gtaaaatcta agatgtttat gcatttctag atgttaattc ttcgctgctt aagaattatc
     4561 ttgatggtaa atctagttgt gccatgcatt ttctcatgat gaatctaagg tgttaattca
     4621 ttgatgtgtg ctgtttcttg ttgcttcatt atcttgatgg tgaatttcgt tgtctaatgc
     4681 actttttcaa atgattattc taattgttac ggcaataact agatggattt tcttagatgt
     4741 tcacttttct agatgtttat acttatcttg atgttttcta aacagaactt ttaaacgctt
     4801 tattttttag tagattgttt aaaatttctt agttgatggt tattgttgtt gatttgaata
     4861 atttgtagcg aatattttgt aacagggcta ctaagaattt ttataacaag aagtgatttc
     4921 aatcgtacta attttcagcg tttgatgaca aaagtgtatg tccaaactta aataatggcg
     4981 ataaaattta agtcatgggt acatttaatg ttttttgatt caaacttcca ttttggtcaa
     5041 tttgcttttt ttataacata gttcaatatc aataaaagat tagcaagtta gttcaaaatg
     5101 gctatgtatt gttttttgaa atacataggt ttgaaatcta tattaattat gcaccaaata
     5161 attt
//
LOCUS       DAXWFK010000165         5086 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.166, whole genome shotgun sequence.
ACCESSION   DAXWFK010000165 DAXWFK010000000
VERSION     DAXWFK010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5086
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.166"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            27..1874
                     /gene="typA"
                     /locus_tag="ACGEXS_002278"
     CDS             27..1874
                     /gene="typA"
                     /locus_tag="ACGEXS_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFF8013263.1"
                     /translation="MTNKREDVRNIAIIAHVDHGKTTLVDELLKQSGIFRENEHVDER
                     AMDSNDIERERGITILAKNTAVDYKGTRINILDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLKPVVVVNKIDKPSARPEGVVDEVLDLFIELEANDEQ
                     LEFPVVYASAVNGTASLDPEKQDDNLQSLYETIIDYVPAPIDNSDEPLQFQVALLDYN
                     DYVGRIGIGRVFRGKMRVGDNVSLIKLDGTVKNFRVTKIFGYFGLKRLEIEEAQAGDL
                     IAVSGMEDINVGETVTPHDHQEALPVLRIDEPTLEMTFKVNNSPFAGREGDFVTARQI
                     QERLNQQLETDVSLKVSNTDSPDTWVVAGRGELHLSILIENMRREGYELQVSKPQVII
                     KEIDGVMCEPFERVQCEVPQENAGAVIESLGSRKGEMVDMTTTDNGLTRLIFNVPARG
                     MIGYTTEFMSMTRGYGIINHTFEEFRPRIKAQIGGRRNGALISMDQGSASTYAILGLE
                     DRGVNFMEPGTEVYEGMIVGEHNRENDLTVNITKTKHQTNVRSATKDQTQTMNRPRIL
                     TLEEALQFINDDELVEVTPESIRLRKKILNKNVREKEAKRIKQMMQENE"
     gene            complement(2014..2181)
                     /locus_tag="ACGEXS_002279"
     CDS             complement(2014..2181)
                     /locus_tag="ACGEXS_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2197 domain-containing protein"
                     /protein_id="HFF8013264.1"
                     /translation="MRQVQCIICDAKVFIDERTTESKRLKNNPIRTFMCDDCKSRLDT
                     PKQRAQHYPLD"
     gene            complement(2183..2665)
                     /locus_tag="ACGEXS_002280"
     CDS             complement(2183..2665)
                     /locus_tag="ACGEXS_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013265.1"
                     /translation="MIQIKGAVKFPITLDSTTWIFDDRKVSIEDLEKGVFDGSKPIDF
                     EDNHEWNRAILEGQTNPPTLNSEIKYKKRAMLEGSFVINMTPFFKHSEPHDSATTIRL
                     SNEHDHLDIPIDLLPYLFFQFAKDGKRLYSDNGVDSFIYNQEDGYSYRINHVTHIEVI
                     "
     gene            2806..3081
                     /locus_tag="ACGEXS_002281"
     CDS             2806..3081
                     /locus_tag="ACGEXS_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1507 family protein"
                     /protein_id="HFF8013266.1"
                     /translation="MAKQATMKNAALKQLTKDADEILHLIKVQLDNLTLPSCPLYEEV
                     LDTQMFGLQKEVDFAVKLGLVDREDGKQIMLRLEKELSKLHEAFTLV"
     gene            3395..4621
                     /gene="ftsW"
                     /locus_tag="ACGEXS_002282"
     CDS             3395..4621
                     /gene="ftsW"
                     /locus_tag="ACGEXS_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFF8013267.1"
                     /translation="MKNFRSILRYIGKTSKFIDYPLLVTYIVLSLIGLVMVYSASMVP
                     ATKGTLTGGIDVPGTYFYNRQLAYVIMSFIIVFFIAFLMNVKLLSNIKVQKGMIITIV
                     SLLLLTLVIGKDINGSKSWINLGFMNLQASELLKIAIILYIPFMISKKMPRVLSKPKL
                     ILSPIVLALGCTFLVFLQKDVGQTLLILIILVAIIFYSGIGVNKVLRFGIPAVLGFLV
                     VFVIALMAGWLPSYLTARFSTLTDPFQFESGTGYHISNSLLAIGNGGVFGKGLGNSAM
                     KLGYLPEPHTDFIFAIICEELGLIGGLLVITLEFFIVYRAFQFANKTSSYFYKLVCVG
                     IATYFGSQTFVNIGGISATIPLTGVPLPFISFGGSSMISLSIAMGLLLIVGKQIKVDQ
                     QRKKQQQKVDIRRQFN"
BASE COUNT         1745 a          705 c          929 g         1707 t
ORIGIN      
        1 acttaagaaa gaaggaaact atttatatga ctaataaaag agaagatgtc cgcaatatag
       61 caattattgc tcacgttgac catggtaaaa caactttagt agatgagttg ttaaaacaat
      121 ctggtatatt cagagaaaat gaacatgtcg atgaacgtgc aatggactct aacgatatcg
      181 aaagagagcg tggaattacg attctagcca aaaatacggc tgttgattat aaaggtacac
      241 gtattaatat tttggataca ccaggacatg cagactttgg tggagaagta gaacgtatta
      301 tgaaaatggt tgatggggtt gtcttagtag tagatgcgta tgaaggtaca atgcctcaaa
      361 cacgttttgt acttaaaaaa gcgctagaac aaaacctgaa acctgttgtt gttgttaata
      421 aaattgataa accatcagca cgtccagagg gtgttgtaga tgaagtttta gatttattta
      481 ttgaattaga agcaaacgat gaacaattag aattccctgt tgtttatgct tcagcagtaa
      541 atggtacagc tagcttagat cctgaaaagc aagatgataa tttacaatca ttatatgaaa
      601 caattattga ttatgtacca gctccaattg ataacagtga tgagccatta caattccaag
      661 tagcattgtt ggactacaat gattatgttg gacgtattgg tattggtcgt gtattcagag
      721 gtaaaatgcg tgtcggagat aatgtatcac taattaaatt agacggtaca gtgaaaaact
      781 tccgtgtaac taaaatcttt ggttactttg gattaaaacg tttagaaatt gaagaagcac
      841 aagctggaga tttaattgct gtttcaggta tggaagacat taatgttggt gaaactgtaa
      901 caccacatga ccatcaagaa gcattgccag ttctacgtat tgatgagcct actcttgaaa
      961 tgacatttaa agttaacaat tctccatttg ctggccgtga aggtgacttt gtaacagcac
     1021 gtcaaattca agaacgttta aatcaacaat tagaaacaga tgtatctttg aaagtttcta
     1081 acacagattc tccagataca tgggtagttg ctggtcgcgg tgaattgcat ttatcaatcc
     1141 ttattgaaaa tatgcgtcgt gaaggttatg aattacaagt ttcaaaacca caagtaatta
     1201 ttaaagaaat agatggtgta atgtgtgaac catttgaacg tgtgcaatgt gaagtgccac
     1261 aagaaaatgc aggtgctgtt attgaatcat taggttcacg taaaggtgaa atggttgata
     1321 tgactacaac tgataatgga cttacacgtt taatctttaa tgtaccggct cgtggtatga
     1381 ttggttatac gactgaattt atgtcaatga caagaggtta cggtattatt aaccatacat
     1441 ttgaagaatt tagaccacgt attaaagcac aaattggcgg tcgtcgtaat ggtgcattaa
     1501 tttcaatgga tcaaggttct gcaagtactt atgccatttt gggacttgaa gatagaggtg
     1561 taaacttcat ggaacctggt actgaagttt atgaaggtat gattgttggt gaacataatc
     1621 gtgaaaatga tttaactgtt aacatcacta aaacaaaaca tcaaactaac gtacgttctg
     1681 caacgaaaga ccaaacacaa acaatgaata gaccgcgtat tctaacattg gaagaagcgt
     1741 tacaattcat taatgatgat gaacttgttg aggttacacc agaaagtata cgtttaagaa
     1801 agaaaatttt aaacaaaaat gttcgtgaaa aagaagcaaa gcgtatcaaa caaatgatgc
     1861 aagaaaacga ataatttaac ataataaaag agaagctgag cgatgaaagt tgcttagctt
     1921 ctcttttggc atataatatt tgaaaaagtg tttaaatgct attatttcct gttactttca
     1981 cattttcaaa tttctaactt gtttattcag atattaatcg agcggatagt gttgcgcacg
     2041 ttgtttaggt gtgtctaatc gacttttaca atcatcacac ataaatgttc gaataggatt
     2101 gtttttaagt cgtttagact cagttgtgcg ctcatctata aatacttttg catcacagat
     2161 aatgcattgc acttgtctca aattaaatca cctcaatatg agttacatgg ttgattcgat
     2221 atgaataacc atcttcttga ttgtaaataa aactatctac accattatcg ctgtaaagtc
     2281 gtttaccgtc ttttgcaaat tggaaaaata aatagggtaa tagatctatc ggtatatcta
     2341 aatgatcatg ctcatttgat aatcgaatag tagttgcaga gtcatgtggt tcggaatgtt
     2401 tgaaaaatgg tgtcatatta atgacaaatg aaccttctaa catggcacgt tttttatatt
     2461 ttatttctga atttaaagta ggcggattag tttgtccttc taggatagca cgattccatt
     2521 catgattatc ttcaaagtcg attggttttg aaccatcaaa tacacctttt tctaaatctt
     2581 cgatgctaac ttttctatca tcgaaaatcc aagtcgtact atctaatgtt ataggaaact
     2641 ttacggctcc tttaatttgt atcattttcc cactccctat caatgtatat agcattattt
     2701 taacacaaat tgctaacgac acattttaaa tctacttgct ttttaatatt tagtaagata
     2761 aacttttagt aagacttgag aatttatata gagggggagc gtgtcatggc gaaacaagca
     2821 acaatgaaaa atgcagcttt gaaacaattg actaaagatg ctgatgaaat cttgcatctg
     2881 attaaagttc aactagataa tttaacatta ccttcatgcc cattatatga agaagtacta
     2941 gatacacaaa tgtttggact tcaaaaagaa gttgattttg ctgttaaatt aggtttagtt
     3001 gaccgcgaag atggcaaaca aattatgtta cgtcttgaga aagaactttc aaaattacat
     3061 gaagctttta cacttgttta aggtttaata tataagcata ttaaaatggc gtgagatatc
     3121 gacaattgag ctgcgccgat tttatattta tagtttaatt actgattaaa gtggctgtta
     3181 tgttgtcttc ttgatgacat gtcagtcact tttattgggt tttagcaatt cacgatatta
     3241 agtaaacaaa tattctataa attacctcaa gagacctgtt aatatttgtg attatgactg
     3301 tgtcatgtat ataataagta acatggattt ttaaaattaa agtataaaac gaaatcattt
     3361 gaagtataaa ttgtagaatt ggatgactga atatatgaag aattttagaa gtattttacg
     3421 gtatattggt aaaacctcaa agtttattga ttatccgtta ttagttacat atattgtatt
     3481 gagtttaatt ggattagtga tggtatatag tgcaagtatg gttccagcaa ctaaaggcac
     3541 attgactggt ggtatcgatg ttccaggaac gtatttttac aaccgacaat tagcatatgt
     3601 cataatgagt tttataattg tattttttat tgcattttta atgaatgtta aattactgag
     3661 taatattaaa gtgcaaaaag gtatgattat aactatcgtc tcactattat tactgacgtt
     3721 agtaataggt aaagatatta atggttctaa aagttggata aacttaggat ttatgaactt
     3781 acaggcatct gagttattaa aaattgcaat catattatat atcccattta tgatcagtaa
     3841 aaaaatgcct agagtattaa gtaaaccaaa attaatttta agtcctattg tattagcatt
     3901 aggttgtacg tttttagttt tcctacaaaa agacgtaggg caaacattac taatattaat
     3961 tattttagtt gcgatcattt tttattcagg aattggggta aacaaagtcc taagatttgg
     4021 tataccagca gtgctaggat tcttagtagt atttgtcatt gcattaatgg ctggttggtt
     4081 accaagttat ttaactgcca gatttagtac gctaacagat ccattccaat tcgaatcagg
     4141 aactggatac catatttcca attcattgct tgcgataggt aacggtggcg tatttggaaa
     4201 aggattagga aatagtgcaa tgaaattggg ctatttacca gaaccacata cagattttat
     4261 ttttgcaatt atttgcgaag aattaggtct aatcggagga ttgctagtta ttactttaga
     4321 gttctttatt gtatatcgtg ccttccagtt tgcaaataaa acatcatcat atttttataa
     4381 acttgtgtgt gttgggattg ccacatactt tggaagtcaa acgtttgtaa acattggcgg
     4441 tatttcggca acaattccat taactggtgt gccattgcca tttatcagct ttggtggatc
     4501 atcaatgatt agtttaagta ttgctatggg tttacttctg attgtaggta aacaaatcaa
     4561 agtagaccag caacgaaaga aacaacaaca aaaagttgat ataagaagac aatttaatta
     4621 aaaaaatact agccaatatt tagtacgaga tgacttctgt cattaagcaa tgctaaacat
     4681 tggctattta tatactcttt gtcaaattgg actgatgagt tcaataacgg aagttaggca
     4741 accaaacaac gcttaacttc ctttttgttt tgatattacg agattactca tgattatcaa
     4801 attttataaa caaaacaacg tataattata actaaatttt atgactaaaa attactaaat
     4861 gtggtggcga aattagaatc atgatgataa gataaagtta tcgctattga taataaatgt
     4921 tgatgttttg caattttatt tttaattttt tagagttgta gaaatcgaaa atgtttcaca
     4981 acaaaaacgt gaactacctg ttgttgcata gtcatttttg aaattattat ttctgaagcg
     5041 gaggttttaa ttgtctcaag aatgttaaat tttgaaaatt tgaaac
//
LOCUS       DAXWFK010000166         5068 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.167, whole genome shotgun sequence.
ACCESSION   DAXWFK010000166 DAXWFK010000000
VERSION     DAXWFK010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5068
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.167"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(146..1852)
                     /gene="acsA"
                     /locus_tag="ACGEXS_002283"
     CDS             complement(146..1852)
                     /gene="acsA"
                     /locus_tag="ACGEXS_002283"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFF8013268.1"
                     /translation="MKVEVYKGAQGKHNLKDYEETYNTFDWKDVEQAFSWSETGKMNM
                     AYECIDRHVDQGLGDKIALNYKDEHRKESYTYKDMQRLSNKAANVLSEHAEVDKGDRV
                     FIFMSRTPELYFALLGVLKIGAIVGPLFEAFMEKAVADRLENSEAKVLITNKALLPRV
                     PVDKLPNLKKIVVVDEDVEDNYIDFISLMETASDEFDIEWLKSDDGLILHYTSGSTGQ
                     PKGVLHVQQAMLVHYISGKYVLDLQEDDVYWCTADPGWVTGTSYGIFAPWLNGATNCI
                     AGGRFSPEQWYSMIEDFKVTIWYTAPTALRMLMSAGDDIVEKYDLSSLRSILSVGEPL
                     NPEVIKWAKKVYGLTVLDTWWMTETGGHMIVNYPTMDVKLGSMGKPLPGIQAAIIDDA
                     GNELPPNRMGNLAIKKGWPSMMYRIWKNPEKYKSYFIGDWYVSGDSAYKDEDGYFWFQ
                     GRVDDVIMTAGERVGPFEVESKLVEHEAVAEAGIIGKPDPVRGEIIKAFVALRKGYEP
                     TDELKEEIRIFVKEGLSAHAAPREIEFKDKLPKTRSGKIMRRVLKAWELNLDAGDLST
                     ME"
     gene            2021..2653
                     /locus_tag="ACGEXS_002284"
     CDS             2021..2653
                     /locus_tag="ACGEXS_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFF8013269.1"
                     /translation="MNHLKTYQSEDYYIHDKQFVIEGPLTHEDLKALTFDAHLTAFRD
                     AEDQYKALLEITTLPEGRIYVARQDQLIVGYVTFHYPDEIERWSTGKLPYLIELGAIE
                     VSINFRQLQLAEKLIQLSLSTPEFENYIVITTEYYWHWDLKNSKLDVFDYKKLMQRLM
                     ATGGLEIFATDDPEITSHPANCLMARIGKNITLEQQQAFDDIRYMNRFFF"
     gene            2678..3847
                     /locus_tag="ACGEXS_002285"
     CDS             2678..3847
                     /locus_tag="ACGEXS_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HFF8013270.1"
                     /translation="MQQHSSKTAYVYSDKLLQYRFHDQHPFNQMRLKLTTELLLNANL
                     LSPEQIVQPRIATDDELMLIHKYDYVEAIKHASHGIISEDEAKKYGLNDEENGQFKHM
                     HRHSATIVGGALTLADLIMSGKVLNGCHLGGGLHHAQPGRASGFCIYNDIAITAQYLA
                     KEYNQRVLIIDTDAHHGDGTQWSFYADNHVTTYSIHETGKFLFPGSGHYTERGEDIGY
                     GHTVNVPLEPYTEDASFLECFKLTVEPVVKSFKPDIILSVNGVDIHYRDPLTHLNCTL
                     HSLYEIPYFVKYLADSYTNGKVIMFGGGGYNIWRVVPRAWSHVFLSLIDQPIQSGYLP
                     LEWINKWKHYSSELLPKRWEDRLNDYTYVPRTKEISEKNKKLALHIASWYESTRQ"
     gene            complement(3945..4934)
                     /gene="ccpA"
                     /locus_tag="ACGEXS_002286"
     CDS             complement(3945..4934)
                     /gene="ccpA"
                     /locus_tag="ACGEXS_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFF8013271.1"
                     /translation="MTVTIYDVAREARVSMATVSRVVNGNQNVKAETKNKVNEVIKRL
                     NYRPNAVARGLASKKTTTVGVIIPDISNIYYSQLARGLEDIATMYKYHSIISNSDNDP
                     EKEKEIFNNLLSKQVDGIIFLGGTITEEMKELINQSSVPVVVLGTNGKDAHIASVNID
                     FTEAAKEITGELIEKGAKSFALVGGEHSKKAQEDVLEGLTEVLNKNGLQLGDTLNCSG
                     AESYKEGVKAFAKMKGNLPDAILCISDEEAIGIMHSAMDAGIKVPEELQIISFNNTRL
                     VEMVRPQLSSVIQPLYDIGAVGMRLLTKYMNDEKIEEPNVVLPHRIEYRGTTK"
     gene            complement(5007..>5068)
                     /locus_tag="ACGEXS_002287"
     CDS             complement(5007..>5068)
                     /locus_tag="ACGEXS_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013272.1"
                     /translation="VFISSFSLNFSKQKYVTVN"
BASE COUNT         1606 a          978 c          745 g         1739 t
ORIGIN      
        1 tcaaatgctt tctacgaaat gatatgacac aagttaacag ttctttatat tgcttatcgt
       61 aaagtaaacg attatttaaa cattgacatg aaatatgttg tcacccttta ttcttcgaaa
      121 aaaatcttca ataacattca tgtcattatt ccattgtact taaatcccca gcatctaaat
      181 ttaattccca agcttttaat acacgtctca taattttacc tgaccgtgtt ttaggtaatt
      241 tatctttaaa ttcgatttca cgtggtgctg catgtgccga caaaccttct ttaacaaata
      301 tacgaatttc ttcttttaat tcgtctgttg gttcatatcc ttttctcagt gcaacaaacg
      361 ccttaattat ttcaccgcga accggatcag gtttaccaat aattcctgct tcggcaactg
      421 cttcgtgttc aaccaattta gactcaacct caaatggtcc aactcgttca ccagctgtca
      481 taattacatc atcaacacgt ccttggaacc agaagtaacc atcttcatct ttatatgccg
      541 aatcaccaga tacataccag tctccaataa aatatgattt atatttttct ggattcttcc
      601 agatacgata catcattgat ggccagcctt tttttatagc aaggttgccc attcgatttg
      661 gtggtaattc attccctgca tcatcgataa ttgcagcttg aataccaggt aatggtttgc
      721 ccattgagcc aagcttgacg tccatcgttg gatagttaac aatcatatgt ccacctgttt
      781 ctgtcatcca ccaagtatct aacaccgtta aaccgtatac ttttttcgcc cattttataa
      841 cttcaggatt taaaggctca cctactgata gaatcgaacg taacgatgac aagtcatatt
      901 tctcaacaat atcgtcacca gcactcatta acattcttaa agctgttggt gccgtatacc
      961 aaatcgtcac tttaaaatct tcaatcatac tataccactg ttctggcgaa aagcgaccac
     1021 cagctataca atttgtagcg ccatttaacc atggtgcaaa aataccataa gatgttcctg
     1081 taacccaacc tggatctgct gtacaccaat aaacatcatc ttcttgtaaa tctaatacat
     1141 attttccaga aatatagtgc actaacattg cttgttgaac atgcaataca cctttaggtt
     1201 gcccagtaga acctgatgta taatgtaaaa tcaaaccatc atccgacttt aaccattcaa
     1261 tgtcaaattc atcgctagca gtttccatca aactaatgaa gtctatgtaa ttgtcttcta
     1321 catcctcatc tacgacaaca atttttttca agtttggtaa tttatctaca ggtactcgag
     1381 gtaacaatgc cttattagta attaacactt tagcttcact gttctctaat ctatccgcaa
     1441 ctgccttttc cataaatgct tcaaataacg gcccaacaat tgcaccaatt tttaaaacac
     1501 ctaacaacgc aaaatatagt tcaggtgtac gcgacataaa tataaatact ctgtcacctt
     1561 tgtcaacttc tgcatgttca gacaaaacat tcgctgcttt attagataac cgttgcatat
     1621 ctttataagt atacgattct tttctgtgct catctttgta atttaacgct attttatccc
     1681 ctaatccttg atctacatgg cgatctatgc attcatatgc catgttcatt tttccagttt
     1741 cactccaaga aaatgcttgt tctacgtctt tccaatcaaa agtattatat gtttcttcat
     1801 aatctttaag gttatgttta ccttgcgctc ctttataaac ttcgactttc atttgaaact
     1861 ccccctttgt tatgtgaaaa cgctttcttt ctcaatatta tacataacat ttctaattat
     1921 tcaaaatttt tacttttatc ctttttctag tattaattat tgtaaatttt ctatataatg
     1981 aatttaatac aatcacgacg tcgattgagg agttgaaaat atgaatcatt taaagacgta
     2041 tcaatccgaa gattattaca ttcatgacaa gcaatttgtt attgaaggtc ctttaacaca
     2101 cgaagatttg aaagcgctta ctttcgatgc gcatttaacc gcatttagag atgctgaaga
     2161 tcagtataaa gctttgttag aaattacaac attaccagaa ggtagaattt atgttgctcg
     2221 ccaagatcaa ctcattgtgg gttatgtcac tttccactat cctgatgaaa ttgagcgctg
     2281 gtctacaggt aagcttccat atttaatcga attgggggca attgaagtca gcatcaattt
     2341 taggcaatta caacttgcag aaaagctgat acaacttagc ctttctacac cagaattcga
     2401 gaattatatc gttataacta cagaatatta ctggcattgg gatttaaaaa attcaaagtt
     2461 agatgtattt gactataaaa aattaatgca gcggttaatg gcaactggtg gacttgaaat
     2521 attcgctaca gatgatccag aaataacaag tcatccagct aattgtttaa tggcaagaat
     2581 tggcaaaaac attacattag aacagcaaca agcgtttgat gatattcgtt atatgaatcg
     2641 gtttttcttt taatttttgg aatatcggag ggattatatg caacaacatt catcaaaaac
     2701 tgcatatgtt tattcagata agttattaca atatcgattt catgaccaac atcccttcaa
     2761 tcaaatgcgt ttaaaattaa caacagagct acttttgaat gcaaatttat tgtctccaga
     2821 acaaatagta caacctagaa ttgcaacaga tgacgaatta atgttaattc ataaatatga
     2881 ttacgtcgaa gctattaagc atgcttcaca tggcattatc agtgaagatg aggctaagaa
     2941 atatggatta aatgatgaag agaatggtca atttaagcat atgcaccgcc atagtgccac
     3001 aattgttgga ggcgctttaa ctttagcaga tcttattatg tcaggcaaag tattaaatgg
     3061 ttgtcactta ggtggtggtt tgcatcacgc tcaacctggt cgagcaagtg gtttttgtat
     3121 atacaatgat attgcaatta ccgcacaata cttagctaaa gaatacaatc aacgcgtttt
     3181 aatcatagat accgatgcac atcatggaga tggtacacaa tggagtttct atgccgataa
     3241 ccatgttact acttattcta tccatgaaac cggaaaattt cttttcccag gctctggtca
     3301 ctatactgag cgcggtgaag atatcggcta tggacacact gtaaatgtcc cacttgaacc
     3361 gtatacagaa gatgcatcat ttttggagtg ttttaaatta acagttgagc ctgtcgtaaa
     3421 gagttttaaa cctgatatta ttctaagcgt aaatggtgtc gatatacatt atcgtgatcc
     3481 actaactcat ctaaattgta cgttacattc attatatgaa attccatatt ttgtaaaata
     3541 tttagctgat tcttatacga atggaaaggt aattatgttt ggtggcggag gctacaatat
     3601 ttggagagtc gtaccacgtg catggagtca tgtattctta agtttaattg atcaaccaat
     3661 tcaaagtggt tatttaccgt tagaatggat taataaatgg aaacattatt catctgaatt
     3721 attacctaaa agatgggaag atcgtttaaa tgattatacc tatgtccccc gcacaaaaga
     3781 aattagtgaa aaaaataaaa aattagcttt acatatagcg agttggtacg aatctactcg
     3841 tcaataagta acctaatctg caacgatttt aaaagataaa taaaaggcat tcttccaaca
     3901 cccatataat ttgggtcgtt agatgaatgc ctaattttgt gaatttattt tgtagttcct
     3961 cggtattcaa ttctgtgagg taaaactaca tttggttctt ctatcttttc atcgttcata
     4021 tattttgtta ataagcgcat ccctactgca ccgatatcat ataatggttg aataacacta
     4081 gaaagttgtg gtctaaccat ctcaactaat cgtgtattat tgaaactaat aatttgtaat
     4141 tcctctggaa ctttaatacc agcatccatt gcactatgca taataccaat tgcttcttcg
     4201 tcgctgatac ataaaatggc atctggcaaa ttgcctttca ttttggcaaa agcttttacg
     4261 ccttctttat aactttcagc accagaacaa ttcaatgtat cacctaattg aaggccattt
     4321 ttatttaaca cttcagttaa accttctaaa acatcttctt gagctttttt agaatgttct
     4381 ccacctacta aagcaaatga tttagcgcct ttttcaatta attctcccgt aatttctttc
     4441 gcagcttcag taaaatcaat attaactgat gctatatgtg catccttacc atttgttcct
     4501 aatactacta caggtacaga tgattgattt atcaattctt tcatttcttc agtaattgta
     4561 ccaccaagga aaataatacc atcaacctgt ttacttaata agttattaaa aatttctttt
     4621 tccttttcag gatcgttatc tgaatttgaa ataattgagt gatatttata cattgttgca
     4681 atatcttcaa gtccacgagc aagttgtgaa taatagatat tagatatatc tggaatgatc
     4741 acacctactg ttgttgtctt tttactagct aaacctctag caacagcatt tggacgataa
     4801 ttcaaacgct taatgacttc gttaacttta tttttagttt ctgctttaac attttggttc
     4861 ccattaacaa cacgcgacac tgtggccata gagacacgcg cttctcttgc tacatcatat
     4921 atagtaactg tcataatttc ctccttgtaa acgttttatt cattataata acatgttttc
     4981 atttcatttt catagtttac catattttaa tttacagtaa catatttttg tttggaaaaa
     5041 tttagcgaaa agcttgaaat aaaaacaa
//
LOCUS       DAXWFK010000167         4941 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.168, whole genome shotgun sequence.
ACCESSION   DAXWFK010000167 DAXWFK010000000
VERSION     DAXWFK010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4941
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.168"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            31..591
                     /locus_tag="ACGEXS_002288"
     CDS             31..591
                     /locus_tag="ACGEXS_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFF8013273.1"
                     /translation="MTHRALLVVDYSYDFIADDGLLTCGKPGQNIEDFIVSRINDFNY
                     YQDHIFFLMDLHYLHDIHHPESKLFPPHNIVDTSGRELYGKVGKLYETIKAQPNVHFI
                     DKTRYDSFFGTPLDSLLRERSINQVEIVGVCTDICVLHTAISAYNLGYKISVPAEGVA
                     SFNQKGHEWALAHFKNSLGAEVEQHV"
     gene            644..1573
                     /locus_tag="ACGEXS_002289"
     CDS             644..1573
                     /locus_tag="ACGEXS_002289"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFF8013274.1"
                     /translation="MAKTYIFGHKNPDTDAISSAIIMAEFEQLRGNSGAKAYRLGDVS
                     AETQFALDTFNVPAPELLTDDLDGQDVILVDHNEFQQSSDTIASATIKHVIDHHRIAN
                     FETAGPLCYRAEPVGCTATILYKMFRERGFEIKPEIAGLMLSAIISDSLLFKSPTCTQ
                     QDVKAAEELKDIAKVDIQKYGLDMLKAGASTTDKSVEFLLNMDAKSFTMGDYVTRIAQ
                     VNAVDLDEVLNRKEDLEKEMLAVSAQEKYDLFVLVVTDIINSDSKILVVGAEKDKVGE
                     AFNVQLEDDMAFLSGVVSRKKQIVPQITEALTK"
     gene            complement(1768..1941)
                     /locus_tag="ACGEXS_002290"
     CDS             complement(1768..1941)
                     /locus_tag="ACGEXS_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013275.1"
                     /translation="MNVVAVNFKWSFELYMLKVENRAMNRVHNNIYNFNHNEKGRKKT
                     KIYTQHRKYFKKM"
     gene            1993..3372
                     /locus_tag="ACGEXS_002291"
     CDS             1993..3372
                     /locus_tag="ACGEXS_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFF8013276.1"
                     /translation="MNIIEQKFYDSKAFFNTQQTKDISFRKEQLKKLSKAIKSYESDI
                     LEALYTDLGKNKVEAYATEIGITLKSIKIARKELKNWTKTKNVDTPLYLFPTKSYIKK
                     EPYGTVLIIAPFNYPFQLVFEPLIGAIAAGNTAIIKPSELTPNVARVIKRLINETFDA
                     NYIEVIEGGIEETQTLIHLPFDYVFFTGSENVGKIVYQAASENLVPVTLEMGGKSPVI
                     VDETANIKVASERICFGKFTNAGQTCVAPDYILVHESVKDDLITALSKTLREFYGQNI
                     QQSPDYGRIVNLKHYHRLTSLLNSAQMNIVFGGHSDEDERYIEPTLLDHVTSDSAIMQ
                     EEIFGPILPILTYQSLDEAIAFIHQRPKPLSLYLFSEDENATQRVINELSFGGGAIND
                     TLMHLANPKLPFGGVGASGMGRYHGKYSFDTFTHEKSYIFKSTRLESGVHLPPYKGKF
                     KYIKAFFKN"
     gene            complement(3492..4520)
                     /locus_tag="ACGEXS_002292"
     CDS             complement(3492..4520)
                     /locus_tag="ACGEXS_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HFF8013277.1"
                     /translation="MTNGYIGSYTKKNGKGIYRFELNENQSRIDLLETGFELEASTYL
                     VRNNEVLYGINKEGEQCGVASLKIDDNGELHLLNKCLSSKAGTGCYVSISEDKRYLFE
                     AVYGAGIIRMYELNTHTGEIIRLIQELAHDFPTGTHERQDHPHAHYINQTPDGKYVAV
                     TDLGADRIVTYKFDDNGFEFYKESLFKDSDGTRHIEFHDNGKFAYVVHELSNTVSVAE
                     YNDGKFEELERHLTIPENFDGDTKLAAVRLSHDQQFLYVSNRGHDSIAIFKVLDNGQH
                     LELVTITESGGQFPRDFNIASSDDLLVCAHEQGDSVVTVFERNKETGKITLCDNTRVA
                     SEGVCVIF"
     gene            4790..4891
                     /locus_tag="ACGEXS_002293"
                     /pseudo
     CDS             4790..4891
                     /locus_tag="ACGEXS_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500634.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1694 a          824 c          765 g         1658 t
ORIGIN      
        1 tattttatta ttatatagga gagatgttga atgacacatc gcgcactatt agttgttgac
       61 tattcatatg actttatcgc agacgacggc ttactaacat gcggtaaacc tggacaaaat
      121 attgaagatt ttattgtttc tcgtatcaat gactttaatt attatcaaga ccatatattc
      181 tttttaatgg atttacatta tttacatgac attcatcatc ctgaaagtaa attattccca
      241 ccacacaata tcgtagatac aagtggtaga gaattatacg gtaaagtagg taaattatac
      301 gaaacaatta aagcgcaacc taatgtacat ttcattgata aaacgcgcta tgattcgttc
      361 tttggtaccc cgcttgatag tttattgaga gaaagaagta ttaatcaagt cgaaatcgtt
      421 ggtgtatgta ccgatatttg cgtgttacat acagcaattt ctgcatacaa cttaggttat
      481 aaaatttcag tacctgctga gggagtggct tcatttaatc aaaaagggca tgaatgggca
      541 cttgcacatt tcaaaaactc attaggtgca gaggtagaac aacacgttta aatcgtgcta
      601 aaataattat aaagaataca atttacaagg gagatatttg acaatggcta aaacatatat
      661 tttcggacat aagaatccag acactgatgc aatttcatct gcgattatta tggcagaatt
      721 tgaacaactt cgaggtaatt caggagccaa agcataccgt ttaggtgatg tgagtgcaga
      781 aactcaattc gcgttagata catttaatgt acctgctccg gaattattaa cagatgattt
      841 agatggtcaa gatgttatct tagttgatca taacgaattc caacaaagtt ctgatacgat
      901 tgcctctgct acaattaagc atgtaattga tcatcacaga attgcaaatt tcgaaactgc
      961 tggtccttta tgttatcgtg ctgaaccagt tggttgtaca gctacaattt tatacaaaat
     1021 gtttagagaa cgtggctttg aaattaaacc tgaaattgcc ggtttaatgt tatcagcaat
     1081 tatctcagat agcttacttt tcaaatcacc aacatgtaca caacaagatg ttaaagcagc
     1141 tgaagaatta aaagatattg ctaaagttga tattcaaaag tacggcttag atatgttaaa
     1201 agcaggtgct tcaacaactg ataaatcagt tgaattctta ttaaacatgg atgctaaatc
     1261 atttactatg ggtgactatg tgactcgtat tgcacaagtt aatgctgttg accttgacga
     1321 agtgttaaat cgtaaagaag atttagaaaa agaaatgtta gctgtaagtg cacaagaaaa
     1381 atatgactta tttgtacttg ttgttactga catcattaat agtgattcta aaattttagt
     1441 tgtaggtgct gaaaaagata aagttggcga agcattcaat gttcaattag aagatgacat
     1501 ggccttctta tctggtgtcg tttctcgaaa aaaacaaatc gtacctcaaa tcactgaagc
     1561 attaacaaaa taatactata ttactgtcta attatagaca tgttgtattt aactaacagt
     1621 tcattaaagt agaatttatt tcactttcaa tgaactgttt tttatttacg tttgactaat
     1681 ttacaaccct ttttcaatag tagtttttat tcctttagct accctaaccc acagattagt
     1741 gatttctata caattcccct tttgtcttta cattttctta aaatatttgc gatgttgagt
     1801 ataaattttt gttttcttcc tacctttttc gttatgatta aagttataaa tattattatg
     1861 tacacgattc atcgctctat tttcaacttt caacatatat aattcgaaag accatttaaa
     1921 attaacggcc acaacattca aatcaattaa tcgctttttc caaaataatc atataaggag
     1981 gttcttttca ttatgaatat cattgagcaa aaattttatg acagtaaagc ttttttcaat
     2041 acacaacaaa ctaaagatat tagttttaga aaagagcaat taaagaagtt aagcaaagct
     2101 attaaatcat acgagagcga tattttagaa gcactatata cagatttagg aaaaaataaa
     2161 gtcgaagctt atgctactga aattggcata actttgaaaa gtatcaaaat tgcccgtaag
     2221 gaacttaaaa actggactaa aacaaaaaat gtagacacac ctttatattt atttccaaca
     2281 aaaagctata tcaaaaaaga accttatgga acagttttga tcattgcacc atttaactat
     2341 ccttttcaac tagtgttcga acctttaatc ggtgctattg cagcaggtaa tacggcaatt
     2401 attaaaccat ctgagttgac gcctaatgtt gcacgagtga ttaaacggtt aatcaatgaa
     2461 acatttgatg caaattacat tgaagttatt gagggaggaa ttgaagaaac gcaaacgtta
     2521 attcacttac cttttgacta tgtcttcttt acaggaagtg aaaatgtagg caaaatcgtt
     2581 tatcaagctg ccagcgaaaa tttagttcct gtgacattag aaatgggcgg taaatctcca
     2641 gtcatcgttg atgaaacagc gaatattaaa gttgctagtg agcgcatttg ttttgggaaa
     2701 ttcactaatg ccggccaaac atgtgttgca ccagattaca ttttagtaca cgaatctgta
     2761 aaagatgatt taatcacagc cctatcaaaa acgttgcgtg aattttatgg tcaaaatata
     2821 caacaaagtc cagattatgg ccgcattgta aaccttaaac attatcatcg tctgacttca
     2881 ttacttaaca gtgcacaaat gaatattgta tttggtggtc atagtgatga ggatgaacgt
     2941 tatatagaac caacattgtt agatcacgtt acaagtgatt cagcaattat gcaagaagaa
     3001 atttttggtc ctatcttacc gattttaacg tatcagtcat tggatgaagc aatagccttt
     3061 attcaccaaa gaccaaaacc tttgagttta tatttattta gcgaagatga aaatgctaca
     3121 caacgtgtaa taaacgagct atcatttggc ggcggcgcta ttaatgatac attgatgcac
     3181 ctagcgaatc ctaaattacc atttggtggt gttggcgcct caggtatggg acgctatcat
     3241 ggtaaatatt cattcgacac ttttacacat gaaaaaagct acattttcaa atctacacga
     3301 ttagaatcag gtgtccattt accaccatat aaaggtaaat ttaaatacat caaagctttc
     3361 tttaaaaatt aatttttgag acaaaaataa accatgcttc cataatttga tgttcttgaa
     3421 aacaataaat ttcaaggtat acacaaattt tttggaaaca tggttttctt tttatcatga
     3481 ttaattaaag attaaaatat gacacataca ccttcagatg ctacacgagt gttatcacat
     3541 agcgtaattt tacctgtttc tttatttctt tcgaaaacag ttacaactga atctccttgc
     3601 tcatgagcac aaactaaaag gtcatctgat gaggcaatat taaaatctct tgggaattga
     3661 ccaccacttt cagtaattgt tactagttct aagtgttgac cattatcaag aactttaaaa
     3721 attgcaatgc tatcatgccc tctattagat acatataaga attgttgatc atgagataaa
     3781 cgcactgctg caagtttagt atctccatca aagttttcag gaattgttaa atgacgctcg
     3841 agctcttcaa atttaccgtc attatattct gcaacactca cagtatttga taattcgtgt
     3901 acgacataag caaattttcc attatcatga aattcaatat gtcttgtccc atcactatct
     3961 ttaaataaag attctttata aaattcaaac ccgttgtcat caaatttata agtaacgata
     4021 cgatcagcac ctaaatctgt tactgcaacg tacttaccat ctggagtttg attaatataa
     4081 tgtgcgtgtg gatgatcttg tctttcatgt gtacctgttg gaaaatcatg tgcaagttct
     4141 tgaattagac gtataatttc acctgtgtgc gtatttaatt catacatacg tatgatgcca
     4201 gcaccatata ccgcttcaaa taaatatcgt ttatcttctg aaatcgatac ataacaacct
     4261 gtaccagctt ttgaagacaa acatttattt aataaatgca attcaccatt gtcatcaatt
     4321 ttcaaacttg caacaccgca ttgttctcct tctttgttga ttccatataa aacttcatta
     4381 ttacgcacca aatatgtaga cgcttctaat tcaaatcctg tttctaataa atcaatacgt
     4441 gactgatttt cgtttaattc aaaacgataa atccctttac cattcttttt agtgtaagaa
     4501 ccaatatatc catttgtcat tacttttacc cctccatgtt agtcttattt tcattttatt
     4561 aaattttagc tagaattaac aatcatttat catttcctta aagctaaaac tatttaacta
     4621 ctttaaaaga actatttgct gtgatatgtc attattcgat tttaattaat ccctcttttt
     4681 tacttacacg agcattaatt attaacgcgt ttatgcttcc acaaaatttt tgttgcaaaa
     4741 tagaacacat gttcgtataa tgtaattatc agattggggt gaattaagga tgacaagtta
     4801 tgaagaagaa actgattatc gtaacgttcc aagagaatta ctagataaca atatacctat
     4861 gggaagaggc atgattaaat gggctccttt tattatatgt actaatctca taaaaaatct
     4921 attttaatag ggctcaaaca t
//
LOCUS       DAXWFK010000168         4862 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.169, whole genome shotgun sequence.
ACCESSION   DAXWFK010000168 DAXWFK010000000
VERSION     DAXWFK010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.169"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..1184)
                     /gene="sqr"
                     /locus_tag="ACGEXS_002294"
     CDS             complement(<1..1184)
                     /gene="sqr"
                     /locus_tag="ACGEXS_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II sulfide:quinone oxidoreductase Sqr"
                     /protein_id="HFF8013278.1"
                     /translation="MNKHYQIVIIGGGTAGVTVASRLLRKNQNLKEKIAIIDPADHHY
                     YQPLWTLVGAGVSSLKSSRKDMESVIPEGANWIKQAVSSFQPENNSVILGDNTVVYYD
                     FLVVAPGLQINWSSIKGLKENIGKNGVCSNYSPDYVNETWNQISNFKQGNAIFTHPNT
                     PIKCGGAPMKIMYLAEDYFRKHKIRSNANVIYATPKDALFDVGKYNKELERIVEERNI
                     TVNYNYNLVEIDGDKKVATFEHIKAYDRKTISYDMLHVTPPMGPLDVVKESTLSDSEG
                     WVDVNPTTLQHKSYSNVFALGDASNVPTSKTGAAIRKQAPIVANNLLQVMNNQMLTHH
                     YDGYTSCPIVTGYNRLILAEFDYNKNTKETMPFNQAKERRSMYIFKKDLLPKMYWYGM
                     LKG"
     gene            complement(1203..2537)
                     /gene="cstB"
                     /locus_tag="ACGEXS_002295"
     CDS             complement(1203..2537)
                     /gene="cstB"
                     /locus_tag="ACGEXS_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide dioxygenase-sulfurtransferase CstB"
                     /protein_id="HFF8013279.1"
                     /translation="MFFKQFYDNHLSQASYLVGCQRTGEAIIIDPVRDLSKYIEVADS
                     EGLTITQATETHIHADFASGIRDVAKRLNANIYVSGEGEDALGYKNMPSKTQFVKHGD
                     IIQVGNVKLEVLHTPGHTPESISFLLTDLGGGSSVPMGLFSGDFIFVGDIGRPDLLEK
                     SVQIKGSTEISAKQMYESVQNIKNLPDYVQIWPGHGAGSPCGKALGAIPISTIGYEKI
                     NNWAFNEIDETKFIESLTSNQPAPPHHFAQMKQVNQFGMNLYQSYDVYPSLDNKRVAF
                     DLRSKEAFHGGHTKGTINIPYNKNFINQIGWYLDFEKDIDLIGDKSTVEKAKHTLQLI
                     GFDKVAGYRLPKSGISTQSVHSADMTGKEEHVLDVRNDEEWNNGHLDQAVNIPHGKLL
                     NENIPFNKEDKIYVHCQSGVRSSIAVGILESKGFENVVNIREGYQDFPESLK"
     gene            complement(2568..3635)
                     /gene="cstA"
                     /locus_tag="ACGEXS_002296"
     CDS             complement(2568..3635)
                     /gene="cstA"
                     /locus_tag="ACGEXS_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide response sulfurtransferase CstA"
                     /protein_id="HFF8013280.1"
                     /translation="MKQYGEKFIDEFSKAELEKLAKQGQLIDVRTEEEYALGHINGSI
                     LHPVDEIESFNKEKNKTYYVICRSGNRSANASKYLAKQGYNVINLDGGYKAYEEENDS
                     YDTQEEYKSIEIKADRKQFNYRGLQCPGPIVKISQEMKNIEVGDQIEVKVTDPGFPSD
                     IKSWVKQTRHTLVKLDENNNGINAIIQKEKAKDLDINYSAKGTTIVLFSGELDKAVAA
                     LIIANGARAAGKDVTIFFTFWGLNALKKVQTVNVKKQGIAKMFDLMLPKKNIRMPLSK
                     MNMFGLGNMMMRYVMKKKNVDSLPTLINQAIEQNIKLIACTMSMDVMGIQKEELRDEV
                     EYGGVGTYIGATENANHNLFI"
     gene            3772..4032
                     /gene="cstR"
                     /locus_tag="ACGEXS_002297"
     CDS             3772..4032
                     /gene="cstR"
                     /locus_tag="ACGEXS_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide-sensing transcriptional repressor
                     CstR"
                     /protein_id="HFF8013281.1"
                     /translation="MNYDKKMINRINRIQGQLNGIIKMMEEGKDCKDVITQISASKSS
                     LQRLMGIIISENLIECVKAAADDEESSQELINEAVNLLVKSK"
     gene            4032..4787
                     /locus_tag="ACGEXS_002298"
     CDS             4032..4787
                     /locus_tag="ACGEXS_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFF8013282.1"
                     /translation="MDIVNIVIMLLIGVFGGFISGLVGVGGAIIIYPAILLLPPLFGA
                     PAYSAYIASGLTSSQVFFSTLSGSLKARKKTEFSPQLVLYMGGGMIIGSMLGAFLANL
                     FDATFVNTVYIIIALLALTLMFIKVKPSSEKSSFNKYLLVIIGLFIGIISGIVGAGGA
                     FIIIPILLVLFKLPMNTVVANSIVIAFISSIGAFVIKLIQGYIPLYDALFLIIGSIIF
                     APIGLKLSKKVPNIIQKWIISILIVFAIVQLIL"
BASE COUNT         1492 a          874 c          697 g         1799 t
ORIGIN      
        1 ccttttagca tgccgtacca atacatttta ggtaataaat ctttcttaaa tatatacata
       61 cttctacgtt ctttggcctg attaaacggc attgtttctt tagtattttt attataatca
      121 aactctgcaa gtattaacct attatatcca gtaacaatag ggcatgaagt ataaccatca
      181 taatgatgcg ttaacatttg attattcatc acttgcaata aattattagc gacgataggt
      241 gcttgcttac gaatagctgc gcctgttttt gaagtaggta catttgaagc atcaccaagt
      301 gcaaatacat tagagtagct tttatgctgt aatgtggttg ggttaacatc tacccaaccc
      361 tcactatctg aaagtgtact ttcttttact acatctaagg gacccatagg tggtgttaca
      421 tgtaacatat cataacttat tgtttttcta tcgtatgctt tgatatgttc gaatgtagcc
      481 acttttttgt caccgtcgat ttcaacaagg ttataattat aattgactgt tatatttctt
      541 tcttcaacaa tcctttctaa ttctttatta tattttccta cgtcaaataa agcatctttt
      601 ggcgttgcat atatcacatt agcgttagaa cggattttat gtttcctaaa ataatcttca
      661 gctaaataca taattttcat aggcgcacct ccacacttta taggagtgtt tggatgcgta
      721 aaaatggcat ttccttgttt aaaattagaa atttggttcc aagtttcgtt aacatagtca
      781 ggtgaatagt tagagcaaac accattttta cctatatttt cttttagtcc tttaattgaa
      841 gaccaattaa tctgtaatcc tggagctact actaaaaaat cataataaac gactgtatta
      901 tctcctaaaa taacgctatt attttcaggt tgaaaacttg aaacagcctg ttttatccag
      961 ttagcacctt caggtataac actttccata tctttacgag aacttttcaa actagatacc
     1021 cctgcaccaa ccaacgtcca taatggttga tagtaatgat ggtctgctgg atctataatt
     1081 gctattttct cttttaagtt ttgatttttt cttaatagtc ttgatgctac ggtaacacct
     1141 gctgtaccgc caccaataat aacaatttga taatgcttat tcattttttc cacatcctta
     1201 aattatttta atgattctgg aaaatcttga tagccttctc taatattcac cacattttca
     1261 aaacctttgc tttccaatat acccactgca attgaacttc taacacctga ctgacaatgt
     1321 acatatattt tatcctcttt attaaaagga atattttcat ttaataattt accatgcgga
     1381 atattaactg cttgatctaa gtgtccatta ttccactctt catcattacg tacgtctaat
     1441 acatgttctt ctttacctgt catatcagcg ctatgaacgg actgggttga aatgcctgat
     1501 tttggcaaac gatagcctgc taccttatca aacccaatta attgtaaagt gtgtttcgct
     1561 ttctcaacag tagatttatc tccaattaaa tctatatctt tttcaaaatc taagtaccaa
     1621 ccaatttgat taataaagtt tttgttgtat ggtatattga ttgttccttt tgtgtggcca
     1681 ccgtgaaagg cctctttgct acgaagatca aatgctactc tcttattatc taaactagga
     1741 taaacatcat atgattgata taaattcata ccaaactgat taacttgttt catttgtgca
     1801 aaatgatgcg gtggtgctgg ttgatttgat gttaatgatt caataaattt agtctcatca
     1861 atttcattaa atgcccagtt attaattttc tcataaccta ttgtagatat aggtatggca
     1921 cctaatgctt taccacaagg gcttccagca ccatgacccg gccagatttg aacatagtct
     1981 ggtaaatttt taatattttg aacggactca tacatttgtt tcgcgctaat ttctgtagaa
     2041 ccctttattt gaacagattt ttctaataaa tcaggtctac ctatatcacc aacaaaaata
     2101 aagtcaccac taaataatcc catcggaaca cttgaaccac cacctaaatc agtgagtaaa
     2161 aagctaatac tttcaggcgt gtgtcctgga gtatgcagaa cttctaattt aacattgcct
     2221 acttgaatga tatctccatg tttaacaaat tgtgtttttg atggcatatt tttataccct
     2281 aatgcatctt caccttcgcc agacacatat atatttgcat ttaagcgttt agccacatca
     2341 cgaattcctg aagcaaaatc agcatgaata tgtgtttctg tagcttgtgt aattgttaaa
     2401 ccttcagaat ctgcaacttc tatatatttc gataaatcac gaacagggtc tattattatt
     2461 gcctctcctg tacgttgaca acccactaaa tatgatgctt gagataaatg attatcgtaa
     2521 aactgtttaa aaaacatgat aacaactcct tttattaata gatttaatta gataaataaa
     2581 ttatgattcg cattttcagt agcaccaata taagtgccta caccaccgta ctcaacttca
     2641 tctctaagtt cttctttctg aatacccatg acatccatac tcatcgtaca agcgattaat
     2701 ttgatatttt gctcaatagc ttgattgata agtgttggta atgaatcaac atttttcttt
     2761 ttcattacgt agcgcatcat catatttcct aaaccaaaca tattcatttt ggaaagaggc
     2821 attcgtatat tctttttggg caacattaaa tcaaacattt ttgcaatacc ttgcttttta
     2881 acattaactg tttgcacttt ttttaatgca ttaagccccc aaaaagtaaa gaagatagtt
     2941 acatcttttc cagcagctct agcaccattt gcaataatca acgctgctac agctttgtct
     3001 aattctccac taaataatac aattgtagta cctttagcag aataatttat atctaaatct
     3061 tttgcttttt ctttttgaat aatcgcatta attccattgt tattttcatc aagcttaact
     3121 aaagtatgcc ttgtttgttt cacccaactt ttaatgtcac tagggaatcc agggtctgtg
     3181 actttgactt caatttggtc acctacttca atattcttca tttcttgact aatttttaca
     3241 attggccctg gacattgaag accacgatag ttaaattgtt tacgatctgc tttaatttct
     3301 atacttttat attcttcttg tgtatcataa ctatcgtttt cttcttcata agctttataa
     3361 ccaccatcaa gatttataac gttataacct tgtttagcta aatatttact agcattagca
     3421 cttctgttac cacttctaca gattacataa taggttttat ttttttcttt attgaatgac
     3481 tcaatctcat caacaggatg aagtatggaa ccattgatat gtcctaatgc atactcctct
     3541 tctgttctaa cgtcaattaa ttgcccttgc ttggctagtt tttccaattc tgctttacta
     3601 aattcatcga taaacttttc tccgtattgt ttcataataa cctccctaaa aatacctgta
     3661 tgggtatttt aaacagttgg atggaacttg tcaaatacct ctaggggtat ttgacacatt
     3721 tgtgtttatc tattaatata tttttattca attaaaagtg gaggtatgac tatgaattat
     3781 gataaaaaaa tgattaatcg tattaataga atacaagggc aactaaatgg aattattaaa
     3841 atgatggagg aaggaaaaga ctgtaaagat gtcattacac aaataagtgc atcaaagagt
     3901 tcactccaac gcttgatggg tatcattatt agtgagaatt taatagaatg tgtaaaagca
     3961 gctgcggatg atgaagaaag ctcccaagag ttaattaatg aagctgtaaa cttattggtg
     4021 aaaagtaagt aatggatatt gttaatatag ttatcatgtt actcattggg gtattcggtg
     4081 ggttcatatc tggtctagta ggcgtaggtg gtgctatcat tatttatcca gctattttat
     4141 tattaccacc attattcgga gcccctgcat atagcgcata tatcgcatca ggtcttactt
     4201 ctagccaagt ctttttcagt acattaagtg gctcattaaa agctcgaaaa aaaacagagt
     4261 tctcgccaca attagttctt tatatgggtg gcggcatgat tatagggagt atgttaggtg
     4321 catttctagc aaatttattt gatgctacat ttgtaaatac ggtatatatc attattgctt
     4381 tacttgcatt aacattaatg tttattaaag tgaaacctag ttccgaaaag tcttcattta
     4441 ataaatattt attagttatc attggccttt tcattggtat tatttcaggt atagtaggtg
     4501 caggtggtgc cttcattatt attcctatat tattggtttt attcaaatta ccaatgaata
     4561 ctgtagtcgc taatagtata gtaattgctt ttatatcttc aattggtgct tttgtcatca
     4621 aacttattca aggttatatc cctttatatg acgccttatt ccttataata gggagtatta
     4681 tatttgctcc aatagggtta aaattaagta aaaaagtccc taacataata caaaaatgga
     4741 ttatcagtat tttaatcgtt tttgctatag ttcaactcat tttataaaac taatcctttt
     4801 aactcagaca gcaatttccc gaaaaataat acctcttatt taaacaaata tgtatgcagt
     4861 tc
//
LOCUS       DAXWFK010000169         4856 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.170, whole genome shotgun sequence.
ACCESSION   DAXWFK010000169 DAXWFK010000000
VERSION     DAXWFK010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4856
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.170"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            273..866
                     /locus_tag="ACGEXS_002299"
     CDS             273..866
                     /locus_tag="ACGEXS_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HFF8013283.1"
                     /translation="MILVIDNNDSFTYNLIDYIKTQTKLTVQVVGIDNLLIEDVINMK
                     PKAIVISPGPGNPDDYPILNEVLEQFYQRVPILGVCLGFQCIVSYFGGNIIHGYHPVH
                     GHTTQLRHTNEGIFQGLPQNFNVMRYHSLIADGATFPNCLKITAKNDEAIIMAFEHIR
                     FPVFGVQYHPESILSEYGYRQVELFLSKVGDYCENRI"
     gene            850..2001
                     /locus_tag="ACGEXS_002300"
     CDS             850..2001
                     /locus_tag="ACGEXS_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HFF8013284.1"
                     /translation="MRIEYNYRYYLTENEYKQYHIQLKGFIKKYVATKLADVGEVIHF
                     AQAQQRQGRYVSLYLSYEAAKYFNHAMCTHSLAKDDIYAAAYSFEKAESINSTYEHQT
                     SYVSKYHFSFVESSEVMMTNIKRVQQAIVEGETYQVNYTARLTDNIYYPISTLYERLT
                     QFSNGNYTALLQTDEIQVASISPELFFQKGQFNNVDNVIISKPMKGTMPRGKTEAEDQ
                     QYYKTLQTSSKDRAENVMIVDLLRNDIGRISQSGSIKVYKLFFIEAYKTVFQMTSMVS
                     GTLKNNTDLTQILTSLFPCGSITGAPKLNTMKYIKQLESSPRGIYCGAIGLLLPTEDD
                     KMIFNIPIRTIEYKYGQAIYGVGAGITIDSKPKDEVNEFYAKTKILEML"
     gene            2001..2609
                     /locus_tag="ACGEXS_002301"
     CDS             2001..2609
                     /locus_tag="ACGEXS_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HFF8013285.1"
                     /translation="MQLFETMKIDNGHIPRLTYHTNRIKCSSERLNFKFDEHAWRNEL
                     NDVTTKYHSGQYRLKIVLNAESKFETIVSPLPEKSSFTAKFQVLPKVVNPTFIKNKTT
                     ERKHLAHNHETDLILLTSEDGKVLEFDIGNIVIEEDGKWYTPSYKDDFLKGCMRDYLI
                     DSDKLVEKDFNKNELIYKYHNNEIRLFLINSLREVADVHLCL"
     gene            2675..2881
                     /locus_tag="ACGEXS_002302"
     CDS             2675..2881
                     /locus_tag="ACGEXS_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013286.1"
                     /translation="MIIVYIVLLLILVYVNYRLVNRLLSENRIYVVRLIATITTVISF
                     ILVYALIHELMPFVVRAMDLMYHQ"
     gene            2969..3679
                     /locus_tag="ACGEXS_002303"
     CDS             2969..3679
                     /locus_tag="ACGEXS_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase subunit 1"
                     /protein_id="HFF8013287.1"
                     /translation="MKIYSQGDQAIVVAIEKEVSKSLTEDLLTLRSYLIEQNYPFIIE
                     IVPSESDMMIVYDARDMIKHHNIQSPFLYMKALIESIHLNIKHDFNQQDLIEIPIVYG
                     SKYGPDLESLLKHYKIKLETFIELHSKAQYFVSMMGYSPGFPYLTGLNKKLYINHTSK
                     QKKFIPAGSVVLEGKKCGIVTTDTINDWLVIGYTPLSLFNPKESDFARLKLGDNIKFR
                     PINENELEVGAFKDVNHN"
     gene            3663..4667
                     /locus_tag="ACGEXS_002304"
     CDS             3663..4667
                     /locus_tag="ACGEXS_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HFF8013288.1"
                     /translation="MSIIIEKSGLFSSFQDFGRRGYEHDGVIPCGALDTLAHEIANRL
                     VANDKNEATLEMTNKMATIRFTEPTLIALAGGNVKAYTEHMTISPYKLYLLDKGDVLK
                     FRETSYTSRVYLAVGGGFELDAWLGSNSTDFNVKIGGFKGRTLQDGDEIKLKRDYTAR
                     HHKLFENLAHTKQTDWGIDGYALSFNYMSDVFHVVKNKGTEDFKEDAIQRFVKHDYKV
                     TSKANRMGMMLEGEKIKAFYEDMPPYQTVKKGTIQIKRDGTPIILLNDHYTLGSYPQI
                     GTIASYHLTKLAQKPQGSRLKFQFIDILTAEKNLVKYSNWLNQLFHGIEYRMQLEMMK
                     "
BASE COUNT         1776 a          676 c          866 g         1538 t
ORIGIN      
        1 aaaaaaagcc tatctccata aaagatagac gaaagaaatg ggttgctcct ataatatata
       61 ttaaaggttt accaacgaat gtttgaaatt tcaatgtcta gttttttata gagggtttca
      121 gctaggaacc tctgatattc atttatgtac gatgaataga atataccata tattagttaa
      181 aatcaaggtc aggataatga caatgctttt aaagttttag ctacatccaa tacatattat
      241 aaaaataaaa cacatttaga acggagtata caatgattct agtcatagat aataatgatt
      301 catttacata taatttaata gactatatta agactcaaac gaaactaaca gttcaagttg
      361 ttggtattga taatctgctg atagaagacg tcattaatat gaagccaaaa gcaattgtta
      421 tttcgcctgg gccgggtaat ccggatgatt atcctatctt gaatgaagtg ttagaacaat
      481 tttatcagcg tgtacctata ctaggtgtat gtttaggatt tcaatgtatc gtgtcttatt
      541 ttggtggaaa tatcattcac ggctatcatc ctgtacacgg acatactaca cagttacgcc
      601 ataccaatga aggtattttt caaggactgc ctcaaaattt caatgtaatg cgttatcatt
      661 cattaattgc tgacggagcg acttttccaa attgcttaaa gattacagca aaaaacgatg
      721 aagcgattat tatggcattt gagcatatta gatttccggt ttttggtgtg caatatcatc
      781 ctgaatctat tttgagtgaa tacggttatc gacaagttga attattttta tcgaaggtag
      841 gtgattactg tgagaataga atataattat cgctactatt taactgaaaa tgaatataag
      901 caataccata ttcaattaaa gggatttata aagaagtatg ttgctactaa gttggctgat
      961 gtgggagaag tgatacactt tgcacaagcg cagcaacgac aaggtagata tgtctcgtta
     1021 tatttaagtt acgaagcggc aaagtatttt aatcatgcta tgtgtacaca ttcattagct
     1081 aaagatgata tttatgcagc agcttatagt tttgaaaaag cggaaagcat aaattcaaca
     1141 tatgaacatc aaacttctta tgtatcaaag tatcattttt catttgttga atcttctgag
     1201 gttatgatga ctaatattaa acgtgtccaa caagcaattg ttgaaggcga aacgtatcaa
     1261 gtgaactata cggcgcgctt aacggataac atttattatc ctattagtac tttatatgaa
     1321 cgattaactc aatttagtaa tggtaattat actgcgttat tacaaactga tgaaatccaa
     1381 gtagcgtcta tctcaccaga attatttttt caaaaaggac aatttaacaa tgtcgataac
     1441 gttatcataa gcaaaccgat gaaagggaca atgcctagag gtaaaacgga agctgaagat
     1501 caacagtatt ataaaacatt gcaaacttct tcgaaagatc gtgcagaaaa tgtcatgatt
     1561 gttgatttac taagaaacga tatagggaga atatcacaga gtggctcaat taaggtgtat
     1621 aaactatttt ttattgaggc atataaaact gtatttcaaa tgacttcgat ggtaagtgga
     1681 actttaaaaa ataatacaga cttaactcaa attttaacat cgttatttcc ttgtggttcg
     1741 attacaggtg caccgaaact gaatacaatg aaatatatta aacaattaga aagttcacct
     1801 cgtggtatat actgcggagc aattggacta ttacttccaa ctgaagatga taaaatgatt
     1861 tttaatattc cgattcgcac tattgagtat aaatatggac aagcgattta tggagtcgga
     1921 gcaggtatta caattgattc taagccaaaa gatgaagtga atgaatttta cgcaaaaacc
     1981 aagattttgg agatgttata atgcaattat ttgaaacaat gaaaattgat aatggacata
     2041 tccctagact tacttatcat actaatcgca taaaatgttc ttctgagcga ttaaacttta
     2101 aatttgatga acatgcatgg cgaaatgaat taaacgatgt aacaacaaag tatcacagtg
     2161 gtcaatatag acttaaaatc gtattaaatg ctgaaagcaa atttgaaacg atagtgtcac
     2221 ctttacctga gaaaagtagt tttacagcaa aatttcaagt gttgcccaaa gtagttaatc
     2281 caacttttat aaaaaataaa acgacagaac gaaagcattt agcacacaat catgaaacag
     2341 atttaatatt gctaacttca gaggacggca aggtccttga atttgatatt ggcaacattg
     2401 tcattgaaga ggatggaaaa tggtacacac caagttataa agatgatttc ttaaaaggat
     2461 gcatgcgtga ttatttaata gatagtgaca aacttgttga aaaagacttt aataaaaacg
     2521 aattgattta taaatatcat aacaatgaga tacgtttatt tttgataaat agtttacgag
     2581 aggttgccga tgtccacctt tgcctttaaa attagtatga taataaagta gaaaatgatg
     2641 tgaaaacaaa tttaaaaatt aaatggggcc aaatatgatt attgtttata ttgtgctgtt
     2701 gttaattctt gtatacgtaa attatcgatt agtgaatcga ttgctatctg aaaatagaat
     2761 atatgttgtt cgtttgatag caacaattac tactgttata agctttatcc ttgtatacgc
     2821 attaattcac gaacttatgc cttttgttgt gcgggcaatg gatttaatgt accaccagta
     2881 aatttagtga gtaggtaaaa tgaactattc aatcgattct ataattaaaa atgaaagtaa
     2941 agtaaccaaa tattgaagag gtgtacaaat gaaaatatat agtcaaggtg accaagccat
     3001 tgtagtcgca attgaaaaag aagtatctaa aagtttaacc gaagatttat taacacttcg
     3061 ctcatattta attgaacaaa attatccatt tattatagaa attgtgccat cagaatcaga
     3121 catgatgatt gtctatgacg caagggatat gattaaacac cataatatac aatcaccttt
     3181 tttatacatg aaagcactaa tagaatcgat tcatttaaac ataaaacatg attttaacca
     3241 gcaagatttg attgaaatac caattgtgta tggttcgaaa tatggtccgg atttagaatc
     3301 acttttaaaa cattacaaaa tcaagctaga aacttttatt gaattacatt ctaaggcgca
     3361 atattttgtt tcgatgatgg gatattcacc tgggtttcct tatttaactg gattaaataa
     3421 gaaattgtat attaatcaca cgagtaaaca gaaaaaattc attccagctg gttctgtagt
     3481 acttgaaggg aaaaaatgcg gtattgtaac tacggataca attaatgatt ggttagttat
     3541 tggttataca ccattatcac tttttaatcc gaaagaatca gatttcgcac gcttaaagtt
     3601 aggcgataat attaaattta gacctatcaa tgaaaatgaa ttagaagtag gagcgtttaa
     3661 agatgtcaat cataattgaa aaaagtggct tattcagtag ctttcaggac tttggcagaa
     3721 ggggatatga acatgatggt gtaattccat gtggtgcact tgatacttta gcacatgaaa
     3781 ttgctaatcg attagttgca aatgacaaga atgaagcaac tttggaaatg actaataaaa
     3841 tggcaacgat tcgttttaca gaacctacgc tgattgcatt agcagggggt aatgtcaaag
     3901 cttacactga gcatatgact atatctccat ataaattgta tttgttagat aaaggcgatg
     3961 ttttaaagtt tagagaaaca agttatacat cgcgagtgta tttagctgtg ggaggcggat
     4021 ttgaattaga tgcatggtta ggatctaact caaccgactt taatgtaaaa attggtggtt
     4081 ttaaaggtag aacattacaa gatggcgatg aaataaagct taagagagat tatacagctc
     4141 gtcatcataa gttatttgaa aaccttgctc acacgaaaca aacagattgg ggtattgatg
     4201 gatacgcctt gtcatttaat tatatgtctg atgtatttca tgtcgttaaa aataaaggta
     4261 cggaagattt taaagaagat gccattcaaa gatttgtgaa acatgattat aaagtaacga
     4321 gcaaagcaaa tcgcatgggg atgatgcttg aaggtgaaaa aatcaaagct ttttatgaag
     4381 atatgccacc gtatcagact gtcaaaaaag gaacgataca aattaagcgt gatggcacac
     4441 ctattatcct attaaatgat cattatacgc taggtagcta cccgcaaatc ggtacaatcg
     4501 caagttatca tttaacgaaa ttagcacaaa aaccgcaagg atcacgtttg aaatttcaat
     4561 ttatagatat tttaacggct gaaaagaacc ttgttaagta tagtaactgg ttaaaccaat
     4621 tattccatgg aatagaatat agaatgcaat tagaaatgat gaaataatat ttggtacgta
     4681 gctcatactc gagtccgatg caaataattt cctcctaatg tataatgaaa taatactgtg
     4741 ttttatctgc gaaatgtatc attttctaat tcgtttcaca gtaaaatgaa aagataaagt
     4801 gtgtttttac ttgaattttg actaaaatta ctctatattt attaattgag ctatgc
//
LOCUS       DAXWFK010000170         4825 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.171, whole genome shotgun sequence.
ACCESSION   DAXWFK010000170 DAXWFK010000000
VERSION     DAXWFK010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.171"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            18..344
                     /locus_tag="ACGEXS_002305"
     CDS             18..344
                     /locus_tag="ACGEXS_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HFF8013289.1"
                     /translation="MNLNIETTTQDKFYEVKVGGELDVYTVPELEEVLTPMRQDGTRD
                     IYVNLENVSYMDSTGLGLFVGTLKALNQNDKELYILGVSDRIGRLFEITGLKDLMHVN
                     EGTEVE"
     gene            346..825
                     /gene="rsbW"
                     /locus_tag="ACGEXS_002306"
     CDS             346..825
                     /gene="rsbW"
                     /locus_tag="ACGEXS_002306"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001190828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma B factor RsbW"
                     /protein_id="HFF8013290.1"
                     /translation="MQSKEDFIEMRVPASAEYVSLIRLTLSGVFSRAGATYDDIEDAK
                     IAVSEAVTNAVKHAYKENNNVGIINIYFEILEDKIKIVISDKGDSFDYETTKSKIGPY
                     DKDENIDFLREGGLGLFLIESLMDEVTVYKESGVTISMTKYIKKEQVRNNGERVEIS"
     gene            800..1570
                     /gene="sigB"
                     /locus_tag="ACGEXS_002307"
     CDS             800..1570
                     /gene="sigB"
                     /locus_tag="ACGEXS_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigB"
                     /protein_id="HFF8013291.1"
                     /translation="MAKESKSANEISPEQINQWIKEHQENKNTDAQDKLVKHYQKLIE
                     SLAYKYSKGQSHHEDLVQVGMVGLIGAINRFDMSFERKFEAFLVPTVIGEIKRYLRDK
                     TWSVHVPRRIKEIGPRIKKVSDELTAELERSPSISEIADRLEVSEEEVLEAMEMGQSY
                     NALSVDHSIEADKDGSTVTLLDIMGQQDDHYDLTEKRMILEKILPILSDREREIIQCT
                     FIEGLSQKETGERIGLSQMHVSRLQRTAIKKLQEAAHQ"
     gene            2004..4154
                     /locus_tag="ACGEXS_002308"
     CDS             2004..4154
                     /locus_tag="ACGEXS_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFF8013292.1"
                     /translation="MDNQLINSIIEKYQFSKKQIEAVLTLLEEKNTVPFIARYRKEQT
                     GGLDEVQIKQIDDEYQYMVNLQKRKEEVIKNIEQQGLLTEELKKDILKQNKLQRVEDL
                     YRPFKQKKKTRATEAKRKGLEPLAIWMKARKHEVSIEEKAQQFINEEVQSVEDAIKGA
                     QDIIAEQISDNPKYRTKILKDMYHQGVLTTSKKKNAEDEKGIFEMYYAYSEPIKRIAN
                     HRVLAVNRGEKEKVLSVKFEFDTTSVEDFIARQEINHNNVNRSYILEAIKDSLKRLIV
                     PSIEREIHADLTEKAENHAIDVFSENLRNLLLQPPMKGKQILGVDPAFRTGCKLAVIN
                     PFGTFIAKGVIYPHPPVSKKEAAEKDFVQMVKAYDVQLIAIGNGTASRETEQFVADLI
                     KKHQLPVQFIIVNEAGASVYSASEIARDEFPDFQVEERSAVSIGRRVQDPLSELVKID
                     PKSIGVGQYQHDVNQKALENALTFVVETAVNQVGVDVNTASSSLLQYVSGLSSQIAKN
                     IIAYREENGAIKHNKELSKIKRLGAKTFEQSIGFLRIVDGSEPLDNTSIHPESYKVTY
                     QLLDKLGFGGNDLGSDALKAKLNSLDMDELAIELQVGVPTLEDIIKSLKAPNRDPRDE
                     FETPILKSDVLSIEDLQEGMKLSGTVRNVVDFGAFVDIGVKQDGLVHVSKLSKKFVKN
                     PMDIVSVGDIVDVWIYSIDKNKDKVSLTMIDPHE"
     gene            4147..4602
                     /locus_tag="ACGEXS_002309"
     CDS             4147..4602
                     /locus_tag="ACGEXS_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFF8013293.1"
                     /translation="MNNDKLQRMVENLSEEKFGRTFRHCAYFNKRLRTTGGRYLLKSH
                     DIEINPKQYEHYGEDAVVKIILHELCHYHLHIAGKGYQHKDQDFKRLSQQVGAPRFCN
                     SIESYQQRANYEYYCTKCHAKYIRIRKVDTNRMRCGHCNGKLRMKRQLK"
BASE COUNT         1830 a          608 c          952 g         1435 t
ORIGIN      
        1 agtaggagtg taatgaaatg aatcttaata tagaaacaac cactcaagat aaattttacg
       61 aagttaaagt cggtggagaa ttagatgttt atactgtgcc tgaattagaa gaggttttaa
      121 cacctatgag acaagatgga actcgtgata tttatgttaa tttagaaaat gtgagttata
      181 tggattcgac aggtttaggt ttattcgtag gtacattaaa agcattaaac caaaatgata
      241 aagaactata cattttaggt gtgtcagatc gtatcggtag actatttgaa attactggtc
      301 ttaaggattt aatgcatgtt aatgaaggaa cggaggtcga ataacatgca atctaaagaa
      361 gattttatcg aaatgcgcgt gccagcatcg gcagagtatg taagtttaat tcgtttaaca
      421 ctttctggcg ttttttcgag agctggtgct acatatgatg atattgaaga tgccaagatt
      481 gcagttagtg aagctgtgac aaatgcagtt aaacatgcat acaaagaaaa taacaatgtg
      541 ggcattatta acatatattt tgaaatttta gaagataaaa ttaaaattgt tatttctgat
      601 aaaggtgaca gttttgatta tgaaacaact aaatcaaaaa taggtcctta cgataaagac
      661 gaaaatatag actttttacg cgaaggtggc ctaggtttat ttttaatcga atctttaatg
      721 gatgaagtca cagtatataa agaatctggt gtgacaatca gtatgactaa gtatataaaa
      781 aaagagcagg tgcgaaataa tggcgaaaga gtcgaaatca gctaatgaaa tttcacctga
      841 gcaaattaac caatggatta aagaacacca agaaaataag aatacagatg cacaggataa
      901 gttagttaaa cattaccaaa aactaattga gtcattggca tataaatatt ctaaaggaca
      961 atcacatcac gaagatttag ttcaagttgg tatggttggt ttaataggtg ccataaatag
     1021 attcgatatg tcctttgaac ggaagtttga agccttttta gtacctactg taatcggtga
     1081 aatcaaaaga tatctacgag ataaaacttg gagtgtacat gttccgagac gtattaaaga
     1141 aattgggcca agaatcaaaa aagtgagcga tgaactaacc gctgaattag agcgttcacc
     1201 ttctatcagt gaaatagctg atcgattaga agtctcagaa gaagaagtgt tagaagcaat
     1261 ggaaatggga caaagttata atgcgttaag tgttgatcat tccattgaag ctgataaaga
     1321 tggttcaact gttacgctat tagatattat ggggcaacaa gatgaccatt atgacttaac
     1381 tgaaaaacgt atgattttag aaaaaatatt acctatatta tctgatcgcg aacgagaaat
     1441 catacaatgt acgtttattg aaggattgag tcaaaaagag acaggtgagc gtatcggttt
     1501 aagtcaaatg catgtatcac gacttcagag aacggcaatt aagaaattac aagaagcagc
     1561 acatcaatag aatttgttta ttaatgatac gttttataat gaaaaatcca tataattatc
     1621 ccttgattat taaattgaaa tcgaggggta tttttaattt aattaagatt ttcgaattaa
     1681 tacattatta gtgtagttta atgtgtatcc acataaatgt cgcgatatag tattaataat
     1741 ttaagtgaag aagatatcta attgtcgttt taaataggtg ggttgctatt agaataaaaa
     1801 aagtagtctt agattatgaa atttagaaat gatggtgtgt cattttcaat aatcttagtg
     1861 cgttttaaaa tatagtatga cctaatcatt cgttttaaat gttttggaag tgaaaattac
     1921 attaagtatc ataccttaat agaagtattt tagaatatgt taaaataaat gagtaaattt
     1981 aagaaaaagt gtgggttaag taaatggaca atcaattgat taattcaatc atagagaaat
     2041 atcaatttag taaaaaacaa attgaagcag tattaacact gctagaagaa aaaaatacag
     2101 taccatttat tgcgaggtat cgaaaagagc aaactggtgg actagatgaa gttcaaataa
     2161 agcaaattga tgacgaatac caatatatgg tcaatttaca aaaacgtaaa gaagaagtta
     2221 tcaaaaatat agaacagcaa ggattactta ctgaggaatt aaagaaggat attttaaaac
     2281 agaacaaatt acaacgtgtt gaagacctat ataggccttt taaacaaaag aaaaagacaa
     2341 gggcaactga ggcgaaacgt aaagggttag agccattagc gatatggatg aaggcacgta
     2401 aacatgaagt ctcaattgaa gaaaaagcac aacaatttat aaatgaagaa gtgcaatcgg
     2461 ttgaagatgc tatcaaaggt gcacaagata ttattgcgga acaaatttca gataatccta
     2521 aatatagaac aaaaatttta aaagatatgt atcatcaagg tgtgttaact acatctaaaa
     2581 agaaaaatgc tgaagatgaa aaaggtattt ttgaaatgta ctatgcatat agtgagccaa
     2641 ttaaacgcat tgctaatcat agagttttag ctgttaatcg tggtgaaaaa gagaaagtat
     2701 tatctgtaaa gtttgaattc gatacgacat cagtagagga tttcattgca cgtcaagaaa
     2761 tcaatcataa taatgtaaat cgcagttata ttttagaggc gattaaagat agcttgaaac
     2821 gcttaattgt cccttcgata gagcgtgaaa tccatgctga tttaactgaa aaagctgaaa
     2881 atcatgcaat agatgttttt agtgaaaact taagaaatct attactgcaa cctccaatga
     2941 aaggtaaaca aatattaggc gtagatccag catttagaac aggttgtaaa ttagcagtca
     3001 ttaacccatt cggtactttt atagcaaaag gtgtgattta tccgcatcca ccagtttcta
     3061 aaaaagaggc agcagagaag gattttgtac aaatggttaa agcgtatgat gtgcaattaa
     3121 ttgcaattgg caatggtact gcaagtcgtg aaacagaaca atttgttgca gatttaatta
     3181 aaaagcatca gttgccagta caattcatca ttgtcaatga agcgggcgct tcagtatact
     3241 cagcatcaga aattgctaga gatgaatttc ctgattttca agtggaagaa agaagtgccg
     3301 tatcaattgg acgacgcgtt caagatccat taagtgaatt agttaaaatc gatcctaaat
     3361 ctattggtgt cggacaatac cagcatgatg ttaatcaaaa agcactagaa aatgcattaa
     3421 ctttcgttgt tgaaacagca gttaaccaag taggtgttga tgtcaataca gcatcatcat
     3481 cattattaca atatgtgtct ggtctaagtt cgcaaattgc taaaaatata attgcatata
     3541 gagaagaaaa tggcgcaata aagcataata aagagttaag caagattaag cgtttgggtg
     3601 ctaaaacttt tgagcagagt ataggatttt taagaatagt agacggttca gagccattgg
     3661 ataatacatc gattcatccc gaaagctata aagtaacgta tcagttatta gataaactcg
     3721 gatttggtgg caatgattta ggtagtgatg cattaaaagc taaattaaat tcattggata
     3781 tggatgaact ggcgattgaa ttacaagtcg gtgtacctac attagaggat attattaaat
     3841 ccttgaaagc acctaatcga gatccaaggg acgagtttga aacaccaatc cttaaatccg
     3901 atgtgctatc aattgaagat ttacaagaag gaatgaaatt gagtggtaca gttagaaatg
     3961 tagtagattt tggagcattt gtagatatag gtgttaaaca agatggactt gtccatgttt
     4021 cgaaactttc taagaagttc gtaaaaaatc caatggatat tgtcagtgtt ggtgatatcg
     4081 ttgacgtttg gatttacagt attgacaaaa ataaagacaa agtatcatta acgatgattg
     4141 atccacatga ataatgacaa acttcagcga atggttgaga atctttcgga agaaaaattt
     4201 ggacgtacgt ttcgacattg tgcatatttt aataaaaggt tacgtacaac aggcggacgt
     4261 tatcttttaa agtcccatga tattgaaatt aatccaaaac aatatgagca ttatggagaa
     4321 gatgctgttg taaaaattat actgcacgaa ttgtgtcatt atcacttgca tattgctggt
     4381 aaaggttatc aacataaaga tcaagatttt aaacgattga gccaacaggt tggagcgcct
     4441 agattttgta acagcattga aagctatcaa caacgggcaa attatgaata ttattgcact
     4501 aaatgtcatg caaaatatat acgtatccgt aaagttgata cgaatcgtat gcgttgcgga
     4561 cattgcaatg gaaaattaag aatgaaaaga caattgaaat aagtacaata caaatagagc
     4621 taattcgtgt atatgtaaga ttatttatga gttgtatgga atttatatag aatttatcag
     4681 tgagttattt tgattggtaa acatagtaat tgaataagaa gaaattgtgt ttataaaatg
     4741 tttaaagccg agattgtcgt aatttaatac ttcgcaatct cggcttttct acttatgata
     4801 taggtaatgg cataaattgt ggctt
//
LOCUS       DAXWFK010000171         4773 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.172, whole genome shotgun sequence.
ACCESSION   DAXWFK010000171 DAXWFK010000000
VERSION     DAXWFK010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.172"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..1252
                     /locus_tag="ACGEXS_002310"
     CDS             <1..1252
                     /locus_tag="ACGEXS_002310"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HFF8013294.1"
                     /translation="LGTVGSGVVKIIEENRQQIQDTLNKDIVIKHILVRDKSKKRPLN
                     ISQYHLTEDVNEILNDDSLDIIVEVMGGIEPTVDWLRTALKNKKHVITANKDLLAVHL
                     KLLEDLAEENGVALKFEASVAGGIPIVNAINNGLNANNISKFMGILNGTSNFILSKMT
                     KEQTTFEEALDEAKRLGFAEADPTDDVEGVDAARKVVITSYLSFNQVIKLNDVKRRGI
                     SGVTLTDINVADQLGYKIKLIGKGIYENGKVNASVEPTLIDKKHQLAAVEDEYNAIYV
                     IGDAVGDTMFYGKGAGSLATGSAVVSDLLNVALFFESDLHTLPPHFELKTDKTREMMD
                     SDAEINIKEKSNFFVVVNHVKGSIENFENELKAILPFHRSLRVANYDNQSYAAVIVGL
                     ESSPEELITKHGYEVDKVYPVEGV"
     gene            1258..2319
                     /gene="thrC"
                     /locus_tag="ACGEXS_002311"
     CDS             1258..2319
                     /gene="thrC"
                     /locus_tag="ACGEXS_002311"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFF8013295.1"
                     /translation="MRRWQGLVEEFKAHLPVNENTPKLTLNEGNTPLIHCENMSKILG
                     IDLYVKYEGANPTGSFKDRGMVMAVTKAKEQGKKIVICASTGNTSASAAAYAARAGLK
                     AIVVIPEGKIALGKLSQAVMYGAEIVSIEGNFDEALEIVKEIAKSGEIELVNSVNPFR
                     IEGQKTGSFEIVQQLDGEAPDILAIPVGNAGNITAYWKGFKEYHEAKGSQLPKMFGFQ
                     AEGASPIVQNKVIKNPETIATAIRIGNPASWDKATNALKESNGLIDSVTDDEILEAYQ
                     LMTTKEGVFSEPASNASIAGLIKLHRQGKLPQGKKVVAILTGNGLKDPDTAISLLDNP
                     IKPLPNDKDSIIDYIKGAL"
     gene            2321..3235
                     /gene="thrB"
                     /locus_tag="ACGEXS_002312"
     CDS             2321..3235
                     /gene="thrB"
                     /locus_tag="ACGEXS_002312"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFF8013296.1"
                     /translation="MSNVLELTIPASTANLGVGFDSIGMALDKFLHLSVKETSGTKWE
                     YIFHDDASKQLPTDETNFIYHVAQQVASKYSVDLPNLCIEMRSDIPLARGLGSSASAL
                     VGAIYIANYFGDIQLSKHEVLQLATEIEGHPDNVAPTIYGGLIAGYYNDVSKETSVAH
                     IDIPDVDVIVTIPTYELKTEASRRALPQKLTHSEAVKSSAISNTMICALAQHNYELAG
                     KLMQQDGFHEPYRQHLIAEFDEVKTIASQHNAYATVISGAGPTILIFSRKENSGELVR
                     SLNSQVVSCHSELVDINISGVKERIVYQ"
     gene            3293..4096
                     /locus_tag="ACGEXS_002313"
     CDS             3293..4096
                     /locus_tag="ACGEXS_002313"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFF8013297.1"
                     /translation="MTNYKVVVLDMDDTLLNSDNVISEETANYLTAIQDEGYYVVLAS
                     GRPTEGMIPTARDLKLPEHHSYIISYNGSKTINMTNEEVEVSKSIGKQDFDEIVDYCR
                     DRGFFVLTYHDGQIIYDSEHEYMNIEAELTGLPMKRVDDIKAYIQGDVPKVMGVDYVA
                     NITEARIDLNGVFNDNVDATTSKPFFLEFMAKDVSKGNAIKALCHKLGYSVDQVIAFG
                     DSMNDKSMFEVAGLAIAMGNASDELKQYANEVTLDHNENGIPHALKKLL"
     gene            complement(4389..4703)
                     /locus_tag="ACGEXS_002314"
     CDS             complement(4389..4703)
                     /locus_tag="ACGEXS_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013298.1"
                     /translation="MIGQTNLFDDVLKQDERFVIVVQALEEKNGQLLKRTLREYPGLN
                     HKQMNDLFMHLKELFSEESFAENQSAFSITVYTNLDYTADQIYAHVKRFRGKHDWTQT
                     AK"
     gene            complement(4678..>4773)
                     /locus_tag="ACGEXS_002315"
     CDS             complement(4678..>4773)
                     /locus_tag="ACGEXS_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013299.1"
                     /translation="FFILDILHIVYNIDNNNFSGVKFNDWTNKFI"
BASE COUNT         1714 a          654 c          932 g         1473 t
ORIGIN      
        1 attaggtact gtcggatctg gtgttgttaa aatcatcgaa gagaaccgac agcaaattca
       61 agatacatta aataaagata ttgtcataaa gcatattctt gttcgagata aatctaaaaa
      121 gagaccgcta aatattagcc aatatcattt aactgaagat gttaatgaaa ttttaaatga
      181 tgattcatta gatattatcg ttgaagtcat gggaggaatt gaaccaactg tagattggtt
      241 aagaacagca cttaaaaata aaaaacatgt tattaccgca aataaagatt tattagcagt
      301 acatcttaaa cttttagaag atttagcaga agaaaatggt gtagctttaa agtttgaagc
      361 gagtgtagca ggtggtattc cgatcgtaaa tgccataaat aatggtttga atgcgaataa
      421 tatttcaaaa tttatgggaa ttttaaatgg tacctctaat tttattttat ctaaaatgac
      481 taaagagcaa acgacatttg aggaagcact tgatgaagcg aaaagacttg gttttgctga
      541 agcggatcca actgatgatg tagaaggggt agatgcagcg cgtaaagttg tcattacatc
      601 atatttatca tttaaccaag tcattaaatt aaacgacgtt aaacgaagag gaattagtgg
      661 cgtaacttta actgatatta atgtagccga tcaactgggg tataaaatta aattgattgg
      721 taagggaata tatgaaaatg gcaaagttaa tgcatcggta gaaccaacgt taattgataa
      781 aaagcatcaa ttagcagctg tagaggatga atataacgcg atttatgtta ttggtgatgc
      841 cgttggtgac acgatgtttt atggaaaagg agcaggcagt ttagcaacag gtagtgccgt
      901 tgtcagtgat ttattgaatg tagcattatt ctttgaatca gatttacaca cattgccacc
      961 acattttgaa ttaaagacag ataaaacacg ggaaatgatg gattcagatg cagaaattaa
     1021 tattaaagaa aaatccaatt tctttgtagt agtgaatcat gtcaaaggtt caattgaaaa
     1081 ttttgaaaat gagttaaagg caatattacc atttcaccga tcattaagag ttgcaaatta
     1141 cgataatcaa tcatatgccg ctgttatagt tggattggaa tcatcaccgg aagaattaat
     1201 cactaagcat ggatacgaag ttgacaaagt atacccagta gaaggagttt aattataatg
     1261 agaagatggc aaggattagt agaagagttt aaagcacatt taccagtaaa tgaaaataca
     1321 ccaaaattaa cattgaacga gggaaataca ccactcattc attgtgaaaa tatgtctaaa
     1381 atactaggca tagatttata tgtgaagtat gaaggtgcca atccgacagg ttcatttaaa
     1441 gatcgcggta tggtaatggc tgtgacaaaa gcaaaagagc aaggtaagaa aattgtaata
     1501 tgcgcttcga ctggaaatac atcagcgtct gcagcagcat atgcagcgag agcaggttta
     1561 aaagctatcg tcgtaatacc agaaggtaaa attgcattag gtaaattgtc gcaagcagta
     1621 atgtatggtg cagaaatcgt ttctattgaa ggaaactttg atgaagcttt agaaattgta
     1681 aaagaaattg caaaaagtgg cgaaatcgag cttgtaaact ctgtcaatcc atttagaatc
     1741 gaaggacaaa agacaggctc atttgaaatt gtacaacaat tagacggtga agcacctgat
     1801 attttagcga ttcctgtagg taatgcaggt aatattactg catattggaa aggctttaaa
     1861 gaatatcatg aagctaaagg atcacaattg ccgaaaatgt ttggcttcca agctgaaggc
     1921 gcatcaccaa ttgttcaaaa taaagtcatt aaaaatcctg aaacgattgc aactgctatt
     1981 cgaattggta atcctgctag ttgggataag gcgactaatg ctcttaaaga atcaaatgga
     2041 ttaatagata gtgttactga tgatgaaatt ctagaagcat atcagttaat gacaactaaa
     2101 gaaggtgtct ttagtgaacc agcgagtaat gcttctattg caggtttaat taaattgcat
     2161 agacaaggta aattacctca aggtaaaaaa gtagttgcta ttttaactgg taatggatta
     2221 aaagatcctg atactgctat ttcactacta gataatccga taaagccatt gccaaatgat
     2281 aaagatagca ttatcgatta tattaaagga gctttataac atgtcgaatg ttttggagtt
     2341 aacaattcct gcatcaacag ccaaccttgg agttggcttt gattctatag gtatggcttt
     2401 agataaattt ttgcatctgt ctgtaaagga aacatcaggg acaaaatggg aatatatttt
     2461 ccatgatgat gcatctaagc aattgcctac tgacgaaaca aactttattt atcatgtagc
     2521 acaacaagtt gcttctaaat atagtgttga cttgcctaat ttatgtatcg aaatgagaag
     2581 tgatattcca ttggcaagag ggttaggttc gtcagcttct gctttagtag gagctatata
     2641 tatcgcaaat tattttggtg atatccaact gtctaaacat gaggtattac aattagcgac
     2701 tgaaatcgaa ggacatcctg ataatgttgc gccgaccatt tatggtggtt taatcgctgg
     2761 atattataat gatgtctcga aagaaacgtc agttgcacat atcgacatac cagacgtgga
     2821 tgtgattgta acgataccaa cttatgaact aaaaacagaa gcatcaagac gtgctttacc
     2881 acaaaaatta acacatagtg aagcggttaa aagtagtgca attagtaata caatgatttg
     2941 tgcattagca cagcacaatt atgaattagc aggtaaactc atgcaacaag atggctttca
     3001 tgaaccgtat cgtcagcatt taattgctga atttgatgaa gtgaaaacaa ttgctagtca
     3061 acataatgcc tatgcaactg taattagtgg tgctggacca actattttaa tatttagtcg
     3121 taaagaaaat agtggggaat tggttcgctc tttaaatagt caggtagtat catgccattc
     3181 tgaattggtc gatattaata tcagtggtgt taaagaacga attgtatacc aatagacgct
     3241 ttatattgta aaatagtatt aaatgcagaa tagagaggag atttaatgcg atatgacaaa
     3301 ttataaagtt gtcgttttag atatggatga cacattgcta aattcagata atgtgatatc
     3361 agaagaaact gcaaattatt taacagcaat tcaagatgaa ggttattatg ttgttctagc
     3421 atctggtaga cctactgaag gtatgattcc aactgctaga gatttaaaat tacctgaaca
     3481 tcatagctat attattagtt ataacggtag taaaacgatt aacatgacta atgaagaagt
     3541 agaagtaagt aaatcgattg gtaagcaaga tttcgatgaa attgtagatt attgtcgaga
     3601 tagaggcttt ttcgttctta catatcatga tggtcaaatt atttacgaca gcgaacatga
     3661 gtatatgaat attgaagcag aattaacagg tttaccgatg aaacgtgttg atgatatcaa
     3721 agcgtatatt caaggcgatg tacccaaggt catgggtgta gattatgtag cgaatattac
     3781 agaagctaga attgatttga atggtgtgtt caatgataat gtagatgcta cgacaagtaa
     3841 gccattcttc ttagaattta tggccaaaga cgtttcaaaa ggtaatgcaa ttaaagcgtt
     3901 atgtcacaaa ttgggatatt cggtggatca agtcattgct tttggtgata gtatgaatga
     3961 taaatcaatg tttgaagtcg caggtctagc tattgctatg gggaatgcat cagatgaact
     4021 taagcaatat gcaaatgaag ttacgttgga tcataatgaa aatggtattc cacatgcgct
     4081 caaaaaattg ttataaattt taaaataagc cttaacacat gatatttgaa taagatatct
     4141 tgtggttaag gctttttatt tttgtgaaaa tgacttcagt tatactatgg aggatttgaa
     4201 atacatattt tagattagta atgatatcaa acgaatagag taaatgtata ttttttgaat
     4261 aaatcaagta ttaactagtc acggaaggta gataaataaa tttgatcatt ctgaataata
     4321 acgaatgcat taaaaaatgc agtgacaatc taacattaat tgttaaaatc actgcaagca
     4381 tcttgatttt atttagctgt ttgtgtccag tcatgcttac ctctgaaacg ttttacatga
     4441 gcatatattt ggtcagcagt ataatctaaa tttgtataaa ctgtaatact gaacgctgat
     4501 tggttttcag caaatgattc ttcggaaaat aattccttta agtgcataaa taaatcattc
     4561 atttgtttat ggtttaaacc gggatattcc cttaaagttc tctttaataa ttgtccgttc
     4621 ttttcttcta atgcttgaac tacaataaca aatctttcat cttgctttag tacatcatca
     4681 aataaatttg tttgtccaat cattgaattt cacccctgaa aagttattgt tatctatatt
     4741 atacacgata tgcaaaatat ctaaaataaa gaa
//
LOCUS       DAXWFK010000172         4742 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.173, whole genome shotgun sequence.
ACCESSION   DAXWFK010000172 DAXWFK010000000
VERSION     DAXWFK010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4742
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.173"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            52..654
                     /locus_tag="ACGEXS_002316"
     CDS             52..654
                     /locus_tag="ACGEXS_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013300.1"
                     /translation="MLISLVIPVFALLVIGGIIWMIIEGIVHISKKNKAIDNFFNQVN
                     KVSETYKFATTFLFLILATAGISQFYLYYIVSAFLFWLVILTFGIAGIIFLMPYGLCF
                     LPFYKQKKKKQTFKKYMVYTTIGLSICLGLSLVLVHTTKIYMDEGGVRYYYGSFVMKQ
                     AGGYAYLALAVLSTLLIVAKKATNKNKEIETVDNTNITER"
     gene            921..4073
                     /locus_tag="ACGEXS_002317"
     CDS             921..4073
                     /locus_tag="ACGEXS_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFF8013301.1"
                     /translation="MSQLLNDTLSAWLLIESLSPGEVNFTAEDILSAEHFKNGAKQAQ
                     LQSFDEYFEIWNAERFIISEEKSETGELIFKFYRHCFRYNEINLKIQDIFDDYSEIHN
                     PNGTHCYGYTFNTDKHGKVIVDSIHIPMIMSALKEIEKNKNANIEEKFNDSVEKFVQK
                     VKEILADEPINEFKLKKMDKAYDEYFSVLNSKKDGLFGHYVAIEYVKDSDLPQPEFNS
                     FFISDIEKARKSPNQTLIDYIEGVEESQRIEVDENKEMFDKFLHPSRLPDGRWPSQTE
                     FRLSLMQQLAVNQITSGNERISSVNGPPGTGKTTLLKDIFAHLVVERGKELAKLNNPK
                     DAFVKTKIHETDDKYVYLLKESIAKYKMVVASSNNGAVENISKDLPKIEEIIRNPEKC
                     KFPKYEQNYANLAHELKDFAEIAEDLIGESAWGLFSGVFGKSTNINQVLSHMLKQDAN
                     DIGFAKLLQNENNRMSYNELMSEWQSHQRAFLEELRHVEMLKEESIRAYDVYKNCESF
                     SKIEQVINSEKTSIEEQVYHLDNETLRDNKEIEDLDNRINYIVKQIETLNELIKSIKE
                     SNKGFINKLKAMFNSEEDESYKDHNKEKQQLLTQQLELEKCKKNKHEDLVSKLKEKEK
                     LIKQLTKVQLQLDELNSQLQELEAYRIESKITIPEKDFWSDNNYDERQVTNLWTSDEL
                     QYRRAMLFLRAMILHKLLLIANNTTIYYAINDFKDRRKLIDANPDKVHNAWNVMHLIF
                     PVVSTTFASFKSMYGGIPKDFIDYLFIDEAGQAIPQAAVGALYRSKKVVAVGDPIQIE
                     PVVTLESHLIDNIRKNYHVPEYLVSKEASVQSVADNANQYGFWKSDATDSNQKTWIGI
                     PLWVHRRCLKPMFTIANQIAYNNKMVLPSNITKVGKTGWYDVKGNAVQKQFVKEHGEK
                     VVGLLADDWIEAIKEGKNEPSSFVISPFSAVQQQIKRMLKQQLPTRIDIERTKINQWV
                     DKSIGTVHTFQGKEAQKVYFVIGTDNTQDGAVNWSCEKPNLLNVAVTRAKKEFYVIGD
                     MQRIQMKPFYETIFKERNVK"
     gene            4130..4612
                     /locus_tag="ACGEXS_002318"
     CDS             4130..4612
                     /locus_tag="ACGEXS_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013302.1"
                     /translation="MEKFNNWILNAISGSQTDKNETTEELKGAKFIILYAYSMLVLLA
                     LVISNIFIHILEPKLSITTQIIIVLILIEALIGLRFLKAYDVKRGKDKENKKNSKDFV
                     KLKSILVAILFTSLALTAGTVADIYGFTDLGNTRSDLIVWSIGGIIFGLVCYTMEDKR
                     "
BASE COUNT         1813 a          588 c          868 g         1473 t
ORIGIN      
        1 gattcaattc aatcataatt gtttgaaaat agaggtgagc aggtgaacga tatgttaatt
       61 agtcttgtaa tcccagtttt tgctttgtta gttattggtg gtattatttg gatgattata
      121 gaaggtatag tacatatttc aaaaaagaat aaagcaattg ataacttttt taaccaagtt
      181 aataaagtaa gtgagacata taaattcgct actacttttt tatttctaat cttggctacg
      241 gctggtattt ctcaatttta tctatattat atagtatcag cgtttctttt ttggcttgtc
      301 atacttacct ttggcattgc aggtatcatt tttttaatgc catatggatt atgttttcta
      361 ccgttttata agcaaaaaaa gaaaaaacag acatttaaaa aatacatggt ttacactacg
      421 attggtttgt caatttgtct aggcttatct ctagttttgg ttcacactac gaaaatttat
      481 atggacgaag gtggcgtaag atactattac ggtagttttg taatgaaaca agcgggcggt
      541 tatgcttatt tagctttagc ggtactttca acgttgttaa ttgttgcgaa aaaagctaca
      601 aataaaaata aagaaatcga aaccgtcgac aatacaaata taacggaaag ataattaagg
      661 gagtgctcat tcaggagtgc tcttttttga tgtccaaatt tagttgcaaa tgaaggcata
      721 gaagaaattt ttatagtcat ttattggata atgaattatg gtcatatcga ttaattttat
      781 tagtgaggat tttacaaaca tttgttgaat aaatatggta atgataatat atgaatattt
      841 ttgaaaaaac actaatatga ttaaattatt tgttaaaata gcattaagta aaaaacaaat
      901 agggaacaag gtgggatttc atgagtcaat tgctaaatga tacgttatcg gcttggttgt
      961 taattgaatc tttaagtcca ggagaagtaa attttacagc ggaagatata ctctcagctg
     1021 aacattttaa aaatggtgca aagcaagcac aacttcaaag ttttgatgaa tattttgaaa
     1081 tatggaatgc tgaacgcttt attatatcag aagaaaaatc agagactggg gaacttatat
     1141 ttaaatttta tagacattgc ttccgctata atgaaattaa tttgaaaatt caggatattt
     1201 ttgatgatta ttctgagatt cataatccaa atgggacaca ctgttatggt tacacattta
     1261 acacagataa acacggcaaa gtgatagttg attctataca tattccgatg attatgagtg
     1321 cattaaaaga aattgaaaag aacaaaaatg ccaatataga agaaaaattt aatgattctg
     1381 ttgaaaaatt tgttcaaaaa gtaaaagaaa ttttagcaga tgaaccaatt aatgaattta
     1441 aattgaagaa gatggacaaa gcttatgatg agtacttttc tgtattaaat tcaaagaaag
     1501 atggattatt tggacattat gtagcaatag aatatgtgaa agatagtgat ttaccacagc
     1561 cggaatttaa cagcttcttc ataagtgata ttgagaaagc aagaaaatct cccaaccaaa
     1621 ctttaattga ttacattgaa ggtgtagaag aaagtcagcg catagaagta gatgaaaata
     1681 aagaaatgtt tgacaaattt ttacatcctt cacgtttgcc tgatggacga tggccatcac
     1741 agactgagtt tagattgtct ttaatgcaac aacttgctgt aaaccaaatt acgagtggta
     1801 atgaaagaat aagttcagtt aatgggccac caggtacagg taagactact ttattaaaag
     1861 atatatttgc tcatctagta gttgaaagag gtaaagagtt agctaaacta aataatccta
     1921 aagatgcatt tgtcaaaaca aaaattcatg aaacggatga taaatacgta tacttactaa
     1981 aggaatctat tgccaaatat aagatggtag tcgcatctag taataatgga gctgttgaaa
     2041 atatatctaa agatttaccg aaaattgaag aaattataag aaatcccgaa aaatgtaaat
     2101 tccctaaata tgaacagaat tatgcaaatt tagcacatga attaaaagat tttgctgaaa
     2161 tagctgaaga tttgattggt gaaagtgcct ggggcttatt ttctggagtt tttggtaaaa
     2221 gtactaatat taaccaagta ttgagtcata tgttaaaaca agatgcgaat gatattggct
     2281 ttgctaaatt actacaaaat gagaataatc gtatgagtta taacgagtta atgagtgaat
     2341 ggcaatcaca tcaacgtgca tttttagaag agttgaggca tgttgaaatg ttaaaagaag
     2401 aatctattag agcatatgat gtttataaaa attgtgagtc tttctctaag attgaacagg
     2461 ttattaatag tgaaaaaaca agtattgaag aacaggtata tcatttagat aatgaaacgt
     2521 tacgagacaa taaagaaata gaagatttgg ataatcgaat taattatatt gttaagcaaa
     2581 tagaaacttt aaatgagtta attaaatcca tcaaagaaag caacaaaggt tttattaaca
     2641 aactgaaagc gatgtttaat tcagaagaag atgaaagcta taaagatcat aataaagaga
     2701 agcaacaatt attaacacaa cagttagagt tagagaaatg taaaaaaaac aaacatgaag
     2761 accttgttag caaactaaaa gaaaaagaga aattaattaa acaattaact aaagtacagt
     2821 tgcaattaga cgagttaaat tcacagttac aagagttaga agcatatcgt attgagtcaa
     2881 aaattacaat tccagaaaaa gatttttgga gtgacaacaa ttatgatgag cgccaagtta
     2941 ctaatctgtg gacgagtgac gaacttcaat acagacgtgc catgctcttt ttaagagcaa
     3001 tgatattgca taaattatta ttgattgcta ataatacaac tatttattat gcgattaatg
     3061 attttaaaga tagaaggaaa ttaattgatg caaatccaga taaagtacac aacgcatgga
     3121 atgtgatgca tttaatattt ccagtagtta gtacgacgtt tgcaagcttt aaatctatgt
     3181 atgggggcat accaaaagat ttcatagact acttatttat tgatgaagca ggacaagcaa
     3241 tacctcaagc agctgtggga gcattatatc gttcaaaaaa agttgtagct gtaggtgatc
     3301 cgattcaaat agaaccggtt gtgactttag aaagtcattt aattgataac attcgtaaaa
     3361 attatcatgt tccggaatat ctagtttcta aagaagcttc tgtgcagtct gttgcagaca
     3421 acgccaatca atatggtttt tggaaatctg atgctactga tagtaatcaa aaaacctgga
     3481 taggcatacc tttatgggtg cacagacgat gtttaaaacc tatgttcacg atagctaacc
     3541 aaatcgctta taataataaa atggtgttgc caagtaatat tacaaaagta ggtaaaacag
     3601 gttggtatga cgttaaagga aacgcagttc aaaaacaatt tgtgaaagag catggtgaaa
     3661 aagtagtggg attattagct gatgattgga ttgaagcaat taaggaaggt aaaaatgaac
     3721 cgagctcatt tgtaatatcg cctttttcag cagtacagca acagattaaa cgtatgttaa
     3781 agcaacaact accgactaga attgatattg aacgtacaaa aattaatcaa tgggtcgata
     3841 aatccattgg tactgttcat acttttcaag gtaaagaggc tcagaaggtg tattttgtaa
     3901 taggtactga taatacccaa gatggtgctg tgaactggtc atgcgaaaaa ccaaacttgt
     3961 taaacgttgc agtgacaaga gctaagaaag agttttatgt aattggcgac atgcaaagaa
     4021 tacagatgaa accattttat gagacgattt ttaaagaaag aaatgtaaaa taacatacaa
     4081 aaaagtatat agaggaagtt atacatttta aaaggagcaa aattgaataa tggagaaatt
     4141 taacaactgg atattaaatg caataagtgg atctcaaaca gacaagaatg aaacaactga
     4201 agaattaaaa ggggcaaaat ttatcatttt atatgcatat tcaatgctcg ttttgcttgc
     4261 gttagtaatt tctaacatat tcattcacat tttggagcct aaactatcaa tcaccactca
     4321 aatcatcatc gttttgattt taattgaagc actaattgga ctgcgtttct tgaaagcgta
     4381 cgatgttaag cgtggcaaag ataaagaaaa taagaaaaat agtaaggatt tcgttaaact
     4441 aaaatcaatt ttagtagcaa ttttatttac atcattggcg ctgacagcag gtactgtagc
     4501 tgatatatac ggtttcactg acttaggaaa tactagaagt gatttaatcg tttggagcat
     4561 aggtggtatt atatttggcc tcgtatgtta cacaatggaa gataaaagat aacgataagg
     4621 agctggcgat tataaagcta gctccttttt taacttatat atgtaaagaa ctatcctaag
     4681 ggtttttaat catatgtcaa taatttctat aatacattat taaaacatca attaaataag
     4741 tt
//
LOCUS       DAXWFK010000173         4626 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.174, whole genome shotgun sequence.
ACCESSION   DAXWFK010000173 DAXWFK010000000
VERSION     DAXWFK010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4626
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.174"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(287..1933)
                     /locus_tag="ACGEXS_002319"
     CDS             complement(287..1933)
                     /locus_tag="ACGEXS_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HFF8013303.1"
                     /translation="MNSSSPENPNGKKYSPVFIYSAIVVAIVVLLGAFLPEQFNYVTN
                     NIKMWITEKLGWYYLILTTIIVFFCIFLIFSPIGKLKLGKPNDKPEFNTISWFAMLFS
                     AGMGIGLVFYGAAEPMAHFATPPTADPKTTEAYTEALRSTFFHWGFHAWAVYGVVALA
                     LAYSQFRKGEPGLLSRTLRPLLGDKVEGPIGIFIDVLSVFATIVGVAVSLGMGALQIN
                     GGLHYLFNVPNNTFVQAIIIIVVTILFIASAWSGLSKGIQYLSNLNIGLGTILMVAAL
                     IVGPTVLILNMLTSSTGSLLNTFLFNSFDTAALNPQKREWMSSWTLYYWGWWLSWSPF
                     VGVFIARVSKGRSIREFISGVLLVPAIVSFVWFSVFGVLGIETGKKHKEIFDMTPETQ
                     LFGVFNHVPFGIVLSLIALLLIASFFITSADSATFVLGMQTTFGSLNPSSMVKVVWGI
                     SQALIAFVLLLAGGGNGAEALNAIQSAAIISAFPFSFVVILMMVSFYKDANQERKFLG
                     LTLTPNKHRLQEYIKSQQEDYESDILEKRQSRRNIEKKDN"
     gene            2137..2499
                     /gene="mscL"
                     /locus_tag="ACGEXS_002320"
     CDS             2137..2499
                     /gene="mscL"
                     /locus_tag="ACGEXS_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFF8013304.1"
                     /translation="MLKEFKEFALKGNVLDLAIAVVMGAAFNKIISSLVENIIMPLIG
                     KIFGSVDFAKEWSFWGIKYGLFIQSVIDFIIIAFALFIFVKIANTLMKKEEAEEEAVV
                     EENVVLLTEIRDLLREKK"
     gene            complement(2570..>4626)
                     /locus_tag="ACGEXS_002321"
     CDS             complement(2570..>4626)
                     /locus_tag="ACGEXS_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HFF8013305.1"
                     /translation="IETKIANSKKDISKTNNKILNLDCDKRNIDKEKKMLEENGDLIE
                     SKISFIDKTRVLFNDINKYQQSYLNIERLRTEGEQLGDELNDLIKGLETVEDSIGNNQ
                     SDYEKIIELNNTITNINNEINIIKENEKAKAELDKLLGSKQELENQINEETSILKNLE
                     IKLDRYDKTKLDLNDKESFISEIKSAVNIGDQCPICGNEIQDLGHHIDFDSIAKRQNE
                     IKEIEANIHAIKSNIAVHNSEIKFVNEKISNINIKTQSDFSLEVLNKRLLENENALNN
                     QRDLNKFIEQMKEEKDNLTLQIHNKQLRLNKNESELKLCRDLITEFETLSKYNNITNF
                     EVDYKKYVQDVNQHQELSKEIEDKLMQLSQRKLIEQNNLNHYENQLETYNNDLELNEQ
                     SIEMEMSRLNLTDDNDIDEIIAWRGEQEELEQKRDTYKKRYHEFEMEIARLESLTKDK
                     ELLDSDKLKDEYELKKGKMNTLIDEYSAVHYQCQNNINKTQSIVSHINYLNQELKDQQ
                     EIFQLAEIVSGKNNKNLTLENFVLIYYLDQIIAQANLRLATMSDNRYQLIRREAVSHG
                     LSGLEIDVFDLHSNKSRHISSLSGGETFQSSLALALGLSEIVQQQSGGISLESIFIDE
                     GFGTLDQETLETALDTLLNLKSTGRMVGIISHVSELKNRIPLVLEVKSDQYQSSTRFK
                     RN"
BASE COUNT         1510 a          806 c          620 g         1690 t
ORIGIN      
        1 attgatttat gtcccagcct gagttaattt tctataaaag tatatttaat ttgcgtttat
       61 accgtcaaac ttcactttag ctttgtcaaa cccctttcta ttaagttttc agaaataaac
      121 ctatcttaaa atataaaaaa atcgagaatt cgtagtttaa taacgaaatt ctcgttctta
      181 tccttttgaa tatactcaat tttccacaaa aacaaacaag tagtatatct gttctagcta
      241 ctagaatgac atactacttg ttattaaaat acttaactaa actttattag ttatcttttt
      301 tctctatatt tctacgtgac tgacgctttt caagaatgtc agattcataa tcttcttgtt
      361 gactcttgat atattcttgt aagcgatgtt tattcggagt caatgttaaa cctaggaatt
      421 tacgttcctg gttcgcatcc ttgtagaaac ttaccatcat gagtatgacg acaaaggaga
      481 atgggaatgc acttataatt gcagcacttt gaatcgcatt taaagcttca gcgccgttac
      541 cgccaccagc taataaaagt acaaatgcta ttaaggcctg tgaaattccc caaacaactt
      601 ttaccatact agatggattt aatgaaccaa atgttgtttg cattcctaat acaaatgttg
      661 ctgagtcagc agatgtaata aagaatgatg caattaataa taatgcaatc aacgataaaa
      721 caatgccaaa tggcacatga ttaaacactc caaatagctg tgtttcagga gtcatatcaa
      781 aaatttcttt gtgtttctta cctgtctcga tgcctaatac accaaagaca ctaaaccaaa
      841 caaaactaac aattgctgga actagcaaga caccagaaat gaactctcta attgaacgtc
      901 cttttgaaac tcgtgcaata aacactccaa cgaatggact ccaacttaac caccaacccc
      961 agtaataaag tgtccatgaa gacatccatt cacgtttttg aggatttaaa gctgctgtat
     1021 caaaactatt aaacaagaat gtgtttagta aactacccgt agagctagtt aacatattta
     1081 aaataagaac agttggtcca acaattaaag cagctaccat taaaatagta cctaaaccaa
     1141 tgttcaagtt acttaagtat tgaatacctt tacttaatcc agaccatgca cttgctataa
     1201 ataagatagt aacaacaatg atgataatcg cttgtacaaa cgtattgttt ggaacattga
     1261 acaagtaatg taaaccacca ttaatttgta gagcacccat acctaacgaa acggctaccc
     1321 caacgattgt cgcaaataca gataaaacgt caataaaaat cccaatagga ccttctactt
     1381 tatcacctaa aagaggacgt aaagttctag ataataaacc tggttcacct ttacggaatt
     1441 gcgaatatgc caacgctaac gcaacaacac cataaacagc ccaagcatgg aatccccaat
     1501 ggaaaaatgt tgaacgtaga gcttcagtat aagcttcagt agttttggga tctgctgtag
     1561 gtggcgtagc aaagtgcgcc atcggttcag ctgcaccata aaacaccaaa cctatcccca
     1621 taccagcact aaacaacata gcaaaccatg aaattgtatt aaactcaggt ttgtcatttg
     1681 gtttacctag tttaagtttt ccaataggac taaaaataag gaatatacag aagaacacga
     1741 taatcgtagt aagaataaga taataccaac ctaacttttc tgtaatccac attttaatat
     1801 tattggtaac atagttgaat tgttcaggta aaaatgcacc aagtaatacg actatagcaa
     1861 caacaattgc actatagatg aagactggtg aatacttctt tccatttgga ttctctggtg
     1921 aagaagaatt cataattaat tactcccttc aattctatat ttaattttat gtagtagaat
     1981 aaaaatatta tctaaacatt ttattcaata actcacgcac ctttatagga taacaaaggt
     2041 ctttgcgata atcaaatttt cttgaataat ctattttcta atttttttat ttgttgtata
     2101 attgaggtta catttaaaaa agagaggttg agtgtaatgt taaaagaatt caaagagttc
     2161 gccttaaaag gtaacgtctt agatttagca attgctgttg tgatgggtgc agctttcaac
     2221 aagattatat cttcattagt agaaaatatc attatgccat taattggtaa aattttcgga
     2281 tcagttgatt ttgctaaaga atggtcattc tggggtatta aatacggttt atttatccaa
     2341 tctgttatcg actttattat catcgcgttt gctttattca tctttgttaa gattgcaaat
     2401 acattaatga agaaagaaga agccgaagaa gaagcagttg tggaagaaaa tgttgtgtta
     2461 ttaactgaaa tcagagattt attacgtgag aaaaaataat ttttaaataa aaagagatgg
     2521 gctaaggttg ttctatacat tccaaagtac catctctatt ttttatgttt taatttcttt
     2581 tgaatcttgt tgaactctga tattgatctg atttcacttc taaaactaaa ggtattctat
     2641 ttttcaattc gctcacatgt gaaataatcc caaccattct accagttgat ttaagattta
     2701 ataaagtgtc taacgctgtt tcaagcgttt cttgatctaa tgtaccgaat ccttcatcaa
     2761 taaatattga ttctagtgaa atacctcctg attgctgctg tacaatttcg cttaacccta
     2821 aagctaatgc aagcgacgat tggaaagttt ctccacctga taacgagcta atatgtctag
     2881 acttatttga atgcaaatca aatacatcaa tttctaggcc actaagacca tgagaaaccg
     2941 cttctcgcct aattagttgg tatcgattat ctgacattgt tgctaatctc agatttgctt
     3001 gagcaataat ttgatctaaa tagtaaatta agacaaagtt ttccaatgta agatttttgt
     3061 tattcttacc actgacaatt tcagccaatt gaaatatttc ttgttgatcc tttaattctt
     3121 gatttaagta attaatatgc gaaactatag attgtgtttt attaatatta ttttgacatt
     3181 gataatgaac agcagagtat tcatctatca gtgtattcat ctttcctttt tttagctcat
     3241 attcatcttt taatttatca gagtccaata actctttatc cttagttaat gattctaacc
     3301 tagctatttc catttcaaat tcatgataac gttttttata agtatccctt ttctgctcta
     3361 attcctcttg ctcgcccctc caggctatta tttcatctat atcattgtca tcagttaaat
     3421 tcagcctcga catttccatt tcaatagact gttcattcaa ttctaagtca ttattgtaag
     3481 tttctagttg attttcataa tgatttagat tattttgctc aattaacttt ctttgagaca
     3541 attgcattaa cttatcttca atctcctttg agagttcttg atgctgattc acatcttgaa
     3601 catacttctt ataatccacc tcaaaattag tgatattatt atatttcgag agtgtttcaa
     3661 attcagtgat gagatctcga cataatttca actcagattc atttttattt agacgcaatt
     3721 gtttattatg aatttgcaac gttagattat ctttttcttc tttcatttgt tctataaatt
     3781 tattaagatc tctttgatta ttcaatgcat tttcattttc tagcagacgc ttattcaata
     3841 cttcaagtga aaaatcactt tgcgttttaa tattaatatt cgatattttt tcattaacaa
     3901 atttaatttc agaattatgc acagcaatat tcgattttat agcgtggata tttgcttcaa
     3961 tttctttaat ttcattttga cgtttagcaa tactgtcaaa atcaatatga tgccctaaat
     4021 cctgaatttc attaccacat atcggacatt gatctccaat atttacagca gatttaattt
     4081 cacttataaa gctttcttta tcatttaagt ccaattttgt tttatcgtag cgatctaatt
     4141 ttatttcgag attcttcaat atagatgttt cttcattaat ttgattctct aactcttgct
     4201 tactacctaa tagtttatct aattcagctt tagctttttc attttcttta attatattaa
     4261 tttcattatt tatgttcgtt atcgtattat ttagttcgat aattttctcg taatcacttt
     4321 ggttattacc tattgaatct tcgaccgttt ctaaaccttt aattagatca tttaattcat
     4381 cacctaattg ttcaccctca gttctcaagc gttcaatatt gagataactt tgttgatact
     4441 tattaatatc gttaaataat actctagttt tatcaataaa agagatttta ctttcaatta
     4501 aatctccatt ttcttctaac atttttttct ctttgtctat gtttcgctta tcacaatcaa
     4561 gattcaatat tttattattg gttttagaaa tatctttttt gctattagca attttagttt
     4621 caattt
//
LOCUS       DAXWFK010000174         4585 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.175, whole genome shotgun sequence.
ACCESSION   DAXWFK010000174 DAXWFK010000000
VERSION     DAXWFK010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4585
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.175"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            373..1260
                     /locus_tag="ACGEXS_002322"
     CDS             373..1260
                     /locus_tag="ACGEXS_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8013306.1"
                     /translation="MKKSLIAFILIFMLVLSGCGMKDNDKQGSNDNGSSKSPYHRIVS
                     LMPSNTEILYELGLGKYTVGVSTVDDYPKDVKKGKKQFDALNLNKEELLKAKPDLILA
                     HESQKATANKVLSSLEKQGIKVVYVKDAQSIDETYNTFKQIGKLTHHDKQAEQLVEET
                     KDNIDKVIDSIPAHHKKSKVFIEVSSKPEIYTAGKHTFFNDMLEKLEAQNVYSDINGW
                     NPVTKESIIKKNPDILISTEAKTRSDYMDIIKKRGGFNKINAVKNTRIEVVNGDEVSR
                     PGPRIDEGLKELRDAIYRK"
     gene            1408..2358
                     /locus_tag="ACGEXS_002323"
     CDS             1408..2358
                     /locus_tag="ACGEXS_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFF8013307.1"
                     /translation="MTFNKVLLSWIVILIITTSIYLFWQLGDINDVFNQSILINVRLP
                     RLLEALLTGMILTVAGLIFQTVLNNALADSFTLGLASGATFGSGLALFLGLTTLWIPV
                     FSITFSLITLITVLVITSVLSQGYPVRILILSGLMIGALFNSLLYFLILLKPRKLNTI
                     ANYLFGGFGDAEYSNVSIIAITFIIALFGIFIILNQLKLLQLGELKSQSLGLNVQLIT
                     YIALCIASMITAINVAYVGIIGFIGMVIPQLIRKWQWKQSLGRQLALNIVTGGQIMVM
                     ADFIGSHILSPVQIPASIIIALIGIPVLFYMLISQSKRLH"
     gene            2413..3132
                     /locus_tag="ACGEXS_002324"
     CDS             2413..3132
                     /locus_tag="ACGEXS_002324"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFF8013308.1"
                     /translation="MDLNQIKAVVFDLEGTLLDRVKSREKFIEEQYERFHDYLIHVQL
                     ADFKKAFIELDDDEDNDKPDLYKEIIKRFHVDRLTWKDLFNDFEMHFYRYVFPYYDTL
                     YTLEKLSQKGFQIGVIANGKSKIKQFRLHSLGLMHVINYLSTSETVGFRKPHPKIFED
                     MIDQLGVLPEQIMYVGDDALNDVAPARAMGMVSVWYKQEDAEIEPLEEEVDFTITTVE
                     ELLTILPIKNDNKGENYGSIY"
     gene            3116..3916
                     /locus_tag="ACGEXS_002325"
     CDS             3116..3916
                     /locus_tag="ACGEXS_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFF8013309.1"
                     /translation="MDLFTRKDGTSIHYSTLGEGYPIVLIHTVLDNYSVFNKLAAELA
                     KSFQVVLIDLRGHGYSDKPRHIEIKDFSDDIVELLKYLYIEEVAFVCHEMGGIIGADI
                     SVRYPEFTSSLTLVNPTSIEGELPEERLFRKYAHIIRNWDPEKQDKFLNKRKYYRPRK
                     MNRFLKHVVDTNEISTKEEIQAVKEVFKNADISQTYRNVVVPTKIIAGEFGERTTRLE
                     AKEVADLIQNADFEVYQESSAFPFVEEQERFVEDTAAFINKHHDEKHV"
     gene            4052..4558
                     /locus_tag="ACGEXS_002326"
     CDS             4052..4558
                     /locus_tag="ACGEXS_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013310.1"
                     /translation="MKKLLTASIIACSVVMGVGLVNTSAEAASGNSIDTVKQLIKGDQ
                     SLENVKIGESIKDVLTKYKNPMYSYNEDGTEHYYEFHTKKGMLLVTTDGKKNNGKVTH
                     ISMMYNDANGPTYQAVKNYVGKAVTHTEYSKVAGNFGYIEKGKTTYQFASAPKDKNIK
                     LYRIDLEK"
BASE COUNT         1696 a          596 c          857 g         1436 t
ORIGIN      
        1 tgaagttaga tagatgagtt tatatgaaat ataaaaaata atttactatt ttcttttagt
       61 atgtggactt atataataaa tagaagcata taaagaaaaa aacagttgtt tgtttgtgca
      121 gcaactgcat aagagcccct aatcgctaaa gctcaagggg agtaaaggaa tacagttgtt
      181 tgtgcagcaa ctgcataaaa gcctctaatc actaaaggtg aagaggaacg cagttggatg
      241 ctaaggcaca actgcataaa agcctctaat cgctaaagat gaagaggaac gcagttggat
      301 gctaccgcac aactgcataa atccctctaa tcgctaaagc gaaaagtggg attaaaaagg
      361 agatgtgata gtgtgaagaa atcgttaatt gcttttattt tgatttttat gcttgtcctg
      421 agtggctgtg gtatgaaaga taatgataaa caaggtagca atgataatgg ctcgtctaaa
      481 tcgccgtacc atagaattgt ttcgttaatg cctagtaata ctgaaatttt atatgaatta
      541 ggattaggta aatacacagt tggtgtttca acggttgatg attatccaaa agatgtgaaa
      601 aagggtaaga aacaatttga tgctttgaat ctaaataaag aggaactttt aaaggcaaag
      661 ccagatctaa ttcttgcgca tgagtcgcaa aaggcaactg ctaataaagt attgtcatca
      721 ttagagaaac aaggcatcaa agtagtgtat gttaaagatg cacaatcaat tgatgaaact
      781 tacaacacat ttaagcaaat tgggaaatta acgcatcatg ataagcaggc tgaacaactt
      841 gttgaggaaa ctaaagataa tatcgataaa gtcatagatt caattcctgc tcatcataaa
      901 aaatcaaaag tatttattga ggtttcatca aagcctgaaa tatatacagc agggaagcat
      961 acatttttta atgatatgtt agaaaaatta gaagcccaaa atgtgtatag tgacattaat
     1021 ggttggaacc ctgtaacgaa ggaaagtatt attaaaaaga acccagatat attaatttcg
     1081 acggaagcta agacaagatc agattatatg gatatcatca aaaaaagagg tggattcaat
     1141 aaaattaatg ctgtcaagaa tacacgtatt gaagttgtaa atggtgatga agtatcaaga
     1201 ccaggtccac gtattgatga aggattaaaa gaattaagag atgcaattta tagaaaataa
     1261 accattctaa ttatgcccct tattgctaca tgtaaaaaat acatgtttga gataaggggt
     1321 ttttaaaata tatttagtga atgatagcaa cgcgagtatg tgattgctat aatgaatgta
     1381 attatcgatg aaagaaaaga gaatgctatg acatttaata aagtattatt gagctggata
     1441 gtcatattga ttataacaac tagcatatat ctattttggc agttgggcga tatcaatgat
     1501 gtatttaacc agtctatttt aatcaatgtt agattaccga gattattaga agcattgttg
     1561 acaggtatga tattaactgt tgcaggcctt atatttcaaa cagttttaaa taatgcattg
     1621 gcagatagct ttacattagg attggcaagc ggcgctacat ttggttcagg attagcatta
     1681 tttttaggtt taacaacgtt atggattcct gtattttcaa taacatttag tttgataaca
     1741 ttaataactg tattagtcat tacgtcggta ttgagccaag gctatccagt tagaatctta
     1801 atattaagtg gtttaatgat tggtgcgtta ttcaattcac ttctatattt tttgatttta
     1861 ttaaaacctc gcaaattaaa tacaattgcc aattatctgt ttggtggttt tggtgatgca
     1921 gaatactcaa atgtatctat aatagcaatc acatttatca ttgcattgtt tggtatattt
     1981 atcattctta atcaactaaa gttattgcaa ttaggagaac taaaaagtca gtcactaggc
     2041 ttaaatgttc aattgattac atatatcgcg ttatgtatag cttctatgat aacggcgata
     2101 aatgtcgcat atgttggcat cattggattc attggtatgg tgataccgca actcattaga
     2161 aaatggcagt ggaaacaatc attaggaaga caattggctt taaatattgt aactggagga
     2221 caaataatgg ttatggcaga ttttattggt agccatatat tgtcaccagt acaaataccg
     2281 gcaagtatta tcattgcatt aattggtata ccagtgttat tttacatgct aatatctcag
     2341 tcgaaacggt tacactagca cacgacattt gctaaaataa aaataactat aaacataaag
     2401 agggcataag cgatggattt gaatcaaatt aaagcagttg tatttgattt agaaggtacg
     2461 ttgttggaca gagttaaatc tcgagagaaa tttatcgaag agcaatatga acgatttcat
     2521 gactacttaa ttcatgttca actggcagat tttaaaaaag catttattga gctagatgac
     2581 gatgaagata atgataaacc tgatttatat aaagaaatca ttaaacgttt ccatgtagat
     2641 aggttaactt ggaaagactt atttaatgat tttgaaatgc atttttatcg ttatgtattt
     2701 ccttattacg atactttgta tacactagaa aagctatcgc aaaaaggctt tcaaattggt
     2761 gttatcgcaa atggtaaatc taagattaaa caatttcgat tacattcact tggtttgatg
     2821 catgttatta attatttatc aacatcagaa acagttggtt ttcgtaaacc acatcctaaa
     2881 atttttgaag atatgattga tcaactaggg gtattacctg agcaaattat gtatgttggc
     2941 gatgatgcgt taaatgatgt agctccagca cgagctatgg gcatggttag tgtatggtat
     3001 aaacaagaag atgctgaaat tgaaccactc gaagaagaag ttgattttac aattacaaca
     3061 gtggaagaat tattaaccat tttaccaata aaaaatgata ataaaggaga aaattatgga
     3121 tctatttact agaaaagatg gaacatcgat acattacagt acattaggtg aaggctatcc
     3181 tatcgtattg attcatactg tacttgataa ttattctgtg tttaataaat tagcagcaga
     3241 attagcaaaa tcatttcaag ttgtgttaat tgatttacgt ggacatggct attctgataa
     3301 acctcgtcac attgaaataa aagatttttc tgatgacatt gttgaattac ttaaatattt
     3361 atatattgaa gaagttgcat ttgtatgcca tgaaatgggt ggaatcattg gtgcggatat
     3421 ttcagtacgt tatcctgaat ttacatcatc acttacgttg gtaaatccaa catctattga
     3481 aggtgaatta ccggaagaac gtttatttag aaaatatgcc catattattc gaaactggga
     3541 tcctgaaaaa caagataaat ttttaaataa gcgtaagtat tatcgtccga gaaaaatgaa
     3601 tcgattcctc aaacatgtcg tagatacaaa tgaaatatca actaaagaag aaattcaagc
     3661 agttaaagag gtattcaaaa acgctgatat ttctcaaact tatagaaatg tcgtagtacc
     3721 gacaaaaatt attgcaggag aattcggtga aagaacaaca agattagaag ctaaagaagt
     3781 agctgattta atccaaaatg cggactttga agtatatcaa gaatcaagtg cattcccatt
     3841 tgttgaagag caagaaagat tcgtcgaaga tacagctgca tttatcaaca aacatcacga
     3901 tgaaaagcat gtttaatttt tagtgataag tgaaatataa aagtagtaat tattatttgt
     3961 aatataattg taatatgact gttgttttag aaatgattgt tgtataatgg ttgtgagcac
     4021 aagacgatat aaataaaagg agagagtgct tatgaaaaag ctactaacgg caagtataat
     4081 tgcatgttct gttgtaatgg gagtaggctt agtgaacact agtgccgaag cagcaagtgg
     4141 caactctatt gatactgtta aacaattaat taagggtgat cagtcattag aaaatgtgaa
     4201 aattggcgaa tctattaaag atgttttaac taagtacaaa aatccgatgt attcttacaa
     4261 tgaagatgga actgaacatt attacgaatt ccatactaaa aaaggtatgt tattagtaac
     4321 tactgatggt aagaaaaaca atggtaaagt aactcatatt tcaatgatgt ataatgatgc
     4381 taatggtcca acatatcaag ctgttaaaaa ttatgttggc aaagcagtaa cacatacgga
     4441 atatagcaaa gttgctggta attttggata tattgaaaaa ggcaaaacga cttatcaatt
     4501 tgcctcagca ccaaaagata aaaacataaa attatatcgt attgatttgg aaaaataata
     4561 attaaattaa tcgtgcttaa ccgac
//
LOCUS       DAXWFK010000175         4563 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.176, whole genome shotgun sequence.
ACCESSION   DAXWFK010000175 DAXWFK010000000
VERSION     DAXWFK010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4563
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.176"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            182..1591
                     /locus_tag="ACGEXS_002327"
     CDS             182..1591
                     /locus_tag="ACGEXS_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T7SS effector LXG polymorphic toxin"
                     /protein_id="HFF8013311.1"
                     /translation="MSIDMYLDRSRNQASSVGNLSQTMNSNYDALEKAITQFINDDAL
                     KGKAYTSAKQFFSTVLIPLSTSMKTLSDLTKQACDNFVSRYTSEVDSISLKESELEED
                     IRSLSQQITRYENLNNNLKKHASDNQQAISSNQQIIRTLGQQKHELEEKLRKLREFNQ
                     KSPEIFKEVEEFQKIVQQGLTQAQNFWNFSTNQFNIPSGKELDWAKASHEKYLKVAMG
                     KIEHKAEKETLNKADFAVIKAYAKEHPEDDIPKSILKYINDNKDSIKRDIGLDITSTL
                     LEQGGINASKFGVFINTAGGVKGPAGPNSFVEVKRTSGNVFIENGSKFAKGGKYLGKG
                     VAGVGFGIGMYDDLANDDKTFGEALSHNGMTLAAGSAGTAVGAGLATFVLGSNPVGWV
                     ILAGLAMSTVFALGTDLIYQNNIFGLKDKVDWVGHKIDNSIDVVKKTTEKSMDSVGNA
                     VSEAKNIISNHINPMKWAW"
     gene            1595..2221
                     /locus_tag="ACGEXS_002328"
     CDS             1595..2221
                     /locus_tag="ACGEXS_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFF8013312.1"
                     /translation="MLFNIKVINKNPRYKVVQYNDEYLLIDLVSTWLVYFFPFINWFI
                     PKRYAKLSEKELENLNVDKQNKNNIFWPVTGSSFLFVVILRKYVHTFEVQLDNKILIS
                     LCFIGFIGIAAFYIYLNKKLKLKIYDDNLDNENRVILVPTFKDGSFIVFTYLLLGGCS
                     ILFLIWLMTIKPQNLLVFIMWIIITIFFFLISMGSISNKKVYAKLKKQ"
     gene            2347..2988
                     /locus_tag="ACGEXS_002329"
     CDS             2347..2988
                     /locus_tag="ACGEXS_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HFF8013313.1"
                     /translation="MEILLCEVRVVNKNPRYRIIKYKNDYLMIDLVSTWLVLFFPFIN
                     WLIPKKYVQISREDFDNLNIVKPVKNKALWPAIGSILLFGTMFRDKIYIPDSHLEKNC
                     VIIICSVLLLSILVFYIYLNQKVKLSIYNNRSSNGKIMIFPSFKNLCFVLFSYFFCGG
                     LSIMFLDVLISLSIQNIIVFIAWVIMTMLFFFINMSSIIDKKIHVIYLRSYKY"
     gene            3076..3693
                     /locus_tag="ACGEXS_002330"
     CDS             3076..3693
                     /locus_tag="ACGEXS_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFF8013314.1"
                     /translation="MLCETEIINKNPKYRVIKYDDEYLMVDVIRTWLVYFFPFINWFI
                     PKRCAKISREEYEKLNTVKPVKNKNFWPVVGGTILLGATSRKYIHLLNIQLEKRSVIF
                     ICFVVFLCILIFFVLLNRKLKLKVFDNKKEEQKIILVPTLKNAVLILYGYLLIGGMSI
                     LALSMLLTLENQNLITFIAWGMGLMLFFLMNITLIVNKTVKVIKR"
     gene            3768..4397
                     /locus_tag="ACGEXS_002331"
     CDS             3768..4397
                     /locus_tag="ACGEXS_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HFF8013315.1"
                     /translation="MLCESRVINQNPKYRIIKYNNEYFMVDLVSTWIAYFLPMINWFI
                     PKKYAKISREEFESLNIVKPAKNNTFWPVAGFAVLLTTLTRKYIYLLNIHLEKEIVIL
                     TCCMILLGVFALFIYINTKLKLHIFDKNKSNNEKIILIPTFKNICLSLFAYILFGGLS
                     TMALSMFVTSSPQNIIEFLALIGMTACFFLLNMSSVLDKKIHVILKTNK"
     gene            4454..>4563
                     /locus_tag="ACGEXS_002332"
     CDS             4454..>4563
                     /locus_tag="ACGEXS_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013316.1"
                     /translation="MILNRGAILEQGNKTAKVIKSELITNYIMLATLQIK"
BASE COUNT         1700 a          531 c          779 g         1553 t
ORIGIN      
        1 tatatccata tgctaaaata agaaaaaagg cgacatgtta tctcaattta cctgcatcta
       61 gactccttta cataagaaat aatcatcgcc gagttttaaa attttacaac tttgtagatg
      121 taaaaagcat ttgattttaa atagtttgaa aatcatatat ttttgaaaaa ggagcatgcc
      181 gatgagtatt gacatgtatt tagacagatc tcgaaaccaa gcttcaagtg tggggaattt
      241 gagtcaaaca atgaattcaa attatgatgc gttggaaaaa gcaattactc aatttattaa
      301 tgatgatgcg cttaaaggga aagcgtatac gtcagctaag caatttttta gtacggtgtt
      361 aattccatta tcaacaagta tgaaaacatt gagtgattta acgaagcaag cttgcgataa
      421 ttttgtgtca cgttatacga gtgaggttga tagcatatct ttaaaagaat cagagcttga
      481 agaagatatc agatcattaa gtcaacaaat tacgcgatat gaaaatttga ataacaattt
      541 gaaaaagcat gcttccgata atcagcaagc catttcatcg aaccaacaaa taatacgaac
      601 attaggtcaa caaaaacatg aattagaaga gaagctacgc aaattgcgtg agtttaatca
      661 aaaatcacca gaaatattta aagaagttga agaatttcaa aaaattgtcc aacaaggact
      721 tacccaagcg cagaattttt ggaacttttc aacaaatcaa tttaatattc cttcaggtaa
      781 agaacttgat tgggccaaag caagtcatga aaaatatttg aaagttgcta tggggaaaat
      841 tgaacataaa gcagagaaag aaactttaaa taaagcagac tttgctgtta taaaggcata
      901 tgccaaagaa catccagaag acgatatccc gaaaagtata ttgaaatata taaatgacaa
      961 taaagacagt attaaaagag atataggatt agatattacg tcaacacttt tagagcaagg
     1021 cggtataaat gcaagtaaat tcggtgtatt tatcaataca gcaggtggag tgaaaggccc
     1081 agcaggtcca aattcatttg tggaagtcaa acgtacatca ggtaatgtgt ttatagaaaa
     1141 tggtagtaaa tttgcaaaag gcggaaaata cctaggtaaa ggtgttgctg gtgtaggatt
     1201 tggtataggt atgtatgatg accttgcaaa tgatgataaa acatttggag aggcgttgtc
     1261 gcataatggt atgacgcttg cagctggatc tgcagggaca gcagttggag ctggattagc
     1321 gacttttgtt ttaggaagta atccagtagg atgggtgatt ttagcaggtt tggctatgag
     1381 tacagtattt gcattaggaa cagatttaat ttatcaaaat aatatttttg gattaaaaga
     1441 taaagtagac tgggtagggc ataaaatcga taatagtata gatgttgtta aaaaaactac
     1501 agaaaaatcc atggatagtg ttgggaatgc tgttagtgaa gctaaaaata ttattagcaa
     1561 tcatataaat ccaatgaaat gggcgtggtg agttttgctt tttaatatta aggttattaa
     1621 taaaaatccg agatataaag tcgttcaata taatgatgag tatttgttaa ttgatttagt
     1681 aagcacttgg cttgtatact tttttccttt tattaattgg ttcattccca aaaggtatgc
     1741 aaaacttagt gagaaggaac ttgaaaattt aaatgttgat aaacaaaata aaaataatat
     1801 tttctggcca gttactggta gttcgttttt atttgtagtt attttacgga aatatgtaca
     1861 tacgtttgaa gttcaattag ataataagat tttaatatct ttatgtttta taggatttat
     1921 aggtattgca gcattttaca tttacttaaa taaaaagctt aaactaaaga tatatgatga
     1981 taatctagat aatgaaaata gggttatatt agtgcctact tttaaagatg gaagttttat
     2041 agttttcaca tatctattat taggaggttg ttctatatta tttttaatat ggctaatgac
     2101 tataaaacct caaaatctac tggtatttat tatgtggatt attattacaa tttttttctt
     2161 tctaataagc atgggctcaa ttagtaataa aaaggtttat gccaaactta aaaagcaata
     2221 gaaattacta aacttagatt gtagttcgta agttaagtaa taaacaggaa aatagtatgt
     2281 tactaaaata caaaacactt ataaagtatc tgaaagtgta ttagatataa aaccaacgag
     2341 acggaagtgg agatattgct ttgcgaggtt agagttgtaa acaaaaatcc cagatataga
     2401 attattaaat ataaaaacga ctatctgatg attgatttag taagcacctg gttagttttg
     2461 ttctttccgt ttattaattg gttaatacca aaaaaatatg tccaaatcag cagagaagat
     2521 tttgataatt taaatattgt caaaccagtt aaaaataaag ctttatggcc agctataggt
     2581 agcatacttt tatttggcac tatgtttaga gataaaatat atatacctga ttctcattta
     2641 gaaaaaaatt gcgttattat catttgttcg gttttattat taagtattct agtgttttat
     2701 atatatttaa atcagaaagt aaagttatct atctataata atcggagtag caatggaaaa
     2761 attatgattt ttccatcatt caagaatctt tgctttgtac tattttctta ttttttctgt
     2821 ggtggattat caatcatgtt cttagatgtt ttgattagtt tatctatcca aaatattata
     2881 gtatttattg cttgggttat tatgacaatg ctatttttct ttataaatat gtcttcaata
     2941 atagacaaaa agattcatgt tatatattta aggtcatata aatattaatt ttgatacaaa
     3001 agggcacaag tgttttgaga aacagaaacg ataatatagc tgtgtgaatt tgttattaat
     3061 aggaagtgat aattattgct ttgtgaaacc gaaattatca ataaaaatcc gaaatacaga
     3121 gttattaagt atgatgatga gtatttaatg gtcgatgtaa taagaacttg gcttgtatac
     3181 ttttttccat ttattaattg gttcattcca aaaaggtgcg ccaaaattag tagagaagag
     3241 tatgaaaaac tgaacactgt taagccagtt aaaaataaaa atttttggcc tgttgtagga
     3301 ggaacaatcc tactaggtgc tacttccaga aagtacatac acttacttaa tattcaatta
     3361 gaaaaaagat cggttatatt tatatgcttc gttgtatttc tatgtatttt aatctttttt
     3421 gtgcttctga atcgaaaatt aaaattgaaa gttttcgata ataaaaaaga agaacaaaaa
     3481 ataattttag taccaacatt aaaaaatgct gttcttatat tatatggcta tttgttgatt
     3541 ggaggcatgt caatattagc tttaagcatg ttgctgacat tagaaaacca gaatttaata
     3601 acttttatag cttggggtat ggggttaatg ttgtttttct tgatgaatat aacactcatt
     3661 gtcaataaaa cagtcaaagt tataaaaagg tgaataaata actaattata ttgtattggc
     3721 cgttcttcaa ataaaaaata atttgaataa ggtggaagtg ataaacagtg ctttgcgaat
     3781 ctagagtcat taatcaaaac cctaaatata gaattattaa atacaataat gaatatttca
     3841 tggttgattt agtaagtact tggattgctt attttttgcc catgattaat tggtttattc
     3901 ccaaaaagta cgcgaaaatt agtagagaag aatttgaaag tttaaatatt gtcaaacccg
     3961 ctaaaaataa tactttctgg cctgttgcag gatttgcagt gttattaaca accttaacaa
     4021 gaaaatatat ctatttgctt aacatccatt tagaaaaaga aatagttata ttaacatgct
     4081 gtatgatact tctaggtgtt ttcgcattgt ttatatatat aaatacaaaa ttgaagttac
     4141 atatttttga taaaaataaa agtaataacg aaaagatcat attaatacct acatttaaaa
     4201 atatttgttt atccttattt gcttatatat tatttggtgg attgtcaaca atggctctga
     4261 gtatgtttgt aacttcatcc cctcaaaata taatagaatt tcttgcttta attggcatga
     4321 ctgcatgctt ctttctactg aatatgtcat cggttctaga taaaaaaatt catgttattt
     4381 taaaaacaaa taagtagtaa aattgattaa cttaggtagt atcggatact taaatgttgg
     4441 ttcataaaaa gcaatgattt taaatcgagg agctatctta gaacagggaa ataaaacagc
     4501 caaagttata aaaagtgaat taataactaa ttatattatg ttagccacgc ttcaaataaa
     4561 aaa
//
LOCUS       DAXWFK010000176         4536 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.177, whole genome shotgun sequence.
ACCESSION   DAXWFK010000176 DAXWFK010000000
VERSION     DAXWFK010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4536
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.177"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(153..326)
                     /locus_tag="ACGEXS_002333"
     CDS             complement(153..326)
                     /locus_tag="ACGEXS_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013317.1"
                     /translation="MWNFIKCVFKFVFSLVAITTLVAGVGVVAFAYIFKKDFEDIERK
                     TKEIISDIESKNN"
     gene            777..1817
                     /locus_tag="ACGEXS_002334"
     CDS             777..1817
                     /locus_tag="ACGEXS_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HFF8013318.1"
                     /translation="MQQVTSDIMTFRGSHFELGVKTGKWLQQTPLLKNREKEWKKRVP
                     RFDIDVNETYQIFQTYAPQIWEELMGLQSILKMPTRQIILNFGHYRFTDLKESGCTVF
                     QGKDFMVRNYDYHPATYDGRYLLYQPTDSGLAQIGPVSRVTGRMDGMNESGLTMGYNF
                     MHRKKPANGFVCYMIGRLILENCRNVTEAIQLLKEIPHRSSFSYILMDKSLNHAIVEV
                     TPRSIDVRYDNICTNHFEILTHENRNYTKESKERLARTISQTNDNLDMTTAFKLFNNP
                     QYEIYSKLFKSWSGTIHTSMYQPETLTAYFTLGENAPPEIIDFKSWLDGQDLNITHFT
                     GNIDTDLTFANK"
     gene            complement(1874..2716)
                     /locus_tag="ACGEXS_002335"
     CDS             complement(1874..2716)
                     /locus_tag="ACGEXS_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFF8013319.1"
                     /translation="MKKFFFIGLLVFVVFFTAATIIWFSYDKNKYGTKQYDKTFKDDA
                     FDNVSINLDSTELRIKRGNQFRVKYDGDNDILINIVDKTLKISDKRSKTRGYAIDMNP
                     FHENKKTLTIEMPDKMIKRLNLSSGAGSVRISDVDLENTSIQSINGEVVIKNSNLDAL
                     DSKTNNSSTYISKSNIKNSNIKVVIGTLQIDKSQIKQSIFLNDHGDIEFKNMPSKVDA
                     KASTKQGDIRFKYDSKPEDTILKLNPGTGDSVVKNKTFTNGKVGKSDNVLEFYTIDGN
                     IKVE"
     gene            complement(2713..3270)
                     /locus_tag="ACGEXS_002336"
     CDS             complement(2713..3270)
                     /locus_tag="ACGEXS_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001669025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFF8013320.1"
                     /translation="MNKITYLNELETALDALPRHLRDQKMYEYERYFYEQELNGVDEG
                     EILKKLKDPQDVAAETKARSVIDYAESKPTFENISRAVAASLSLGILSIFVILIPVSI
                     VGLFVLALFLISLLLLFCPIILLASAISRGIVDSISNVFFAISYSGLGLVFIIVIFKI
                     LEYIYRLILKYLLWYIKTVKGSVRK"
     gene            complement(3330..3854)
                     /locus_tag="ACGEXS_002337"
     CDS             complement(3330..3854)
                     /locus_tag="ACGEXS_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013321.1"
                     /translation="MLYLNKYKERVTSVRIQNRWVVFILFLICSFGVLIGLYQYRHTK
                     TVDLSNLEINDIKLNEKFDKKGYEVNKKIKFDRFKFYNSKAHPDLTVKVREKDNIVKG
                     IILVRDEKIHTNFDGGIGSPINNAIENLGFGYKRTKVGNDFSSVKYIDRDNHLKLNLL
                     YQDLEIKRIEFFSK"
     gene            3975..>4536
                     /locus_tag="ACGEXS_002338"
     CDS             3975..>4536
                     /locus_tag="ACGEXS_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFF8013322.1"
                     /translation="MTNLETYFKNSQPLNEYIDGMKTNQDTVLSIYQSFELPEDDSRI
                     KKIKEMNYSKVLVITEDWCGDAMMNLPILKHISEALNLEVRVFHRDDDTKLIDQYLTN
                     GKSRAIPIFVFLNDQFEQETVWGPRANEVQKFVTDVRADKLPSKDHPDYADLEKETHL
                     VISNRYKTDASFWKAVYNSILNKLENK"
BASE COUNT         1628 a          774 c          594 g         1540 t
ORIGIN      
        1 gtcgaagacc aaggctgaga cggcacccta ggaaagcgaa gccattcaat acgaagtatt
       61 gtataaatag agaacagcag taagatattt tctaattgaa aattatctta ctgctgtttt
      121 ttagggattt atgtcccagc ctctaaatgt tattagttat ttttactttc aatatcagaa
      181 ataatttctt tagtttttct ttcaatatct tcaaaatctt ttttaaagat ataagcaaat
      241 gctactacac caacaccagc aactaatgtt gtaatagcaa ctaagctaaa tacgaattta
      301 aacacacatt taataaaatt ccacatctca atttcacctc tgttaaatta aatacctatc
      361 tacatttcat tatactatca tatatagaat aatcattaaa taaataccca ctttcacgaa
      421 atatattcat caaattatat agtgagcaag ctaatgatta ttttcaaaaa tgccgaggaa
      481 cctctgtttc aacgtgacat gaccttatat gctaatgtta acaatgacta ttatcgtgaa
      541 tgctaactta agattgatat ttatgataag atgattttaa gtttttaact caatatcccc
      601 accatctata aaaatatgtt ttaactgatt gataagtctt atacaccgag aaatcacctc
      661 aaacaatttt atatttcatt tagtaaaata taactttatt taacatgaca ttcaatatac
      721 atacgattac tatttattta caatatatta caactaaaaa gaaaggggcc gatgatatgc
      781 aacaagtgac atcagatatc atgactttta gaggttcaca tttcgagtta ggtgttaaaa
      841 ctggtaaatg gcttcaacaa acacctcttc taaaaaatcg agaaaaagaa tggaaaaaac
      901 gtgtcccacg tttcgatata gatgtaaatg aaacttatca aatattccaa acatacgcac
      961 ctcaaatatg ggaagaatta atgggactac aaagtatact caaaatgcca acacgccaaa
     1021 ttattttaaa ctttggacat tatcgtttta ccgatttaaa agaaagtggt tgcacggtct
     1081 ttcaaggcaa agacttcatg gttagaaatt atgattacca ccctgcaact tatgatggtc
     1141 gctacttact ttatcaacct acagatagcg gtttagctca aattggtcct gtatcaaggg
     1201 taactggaag aatggatggt atgaatgagt caggattaac tatgggctat aactttatgc
     1261 accggaagaa accagcaaat ggttttgtat gttatatgat tggtcgtcta attcttgaaa
     1321 attgccgaaa tgtaacagaa gcaatccaat tattgaaaga aataccacac agaagctcat
     1381 ttagctacat actgatggac aaatcattga accatgcaat tgttgaagtg acaccgagat
     1441 caatagatgt tcgttatgat aatatatgta caaatcattt tgaaatatta acacatgaaa
     1501 atcgtaatta tacaaaagaa tctaaagaaa gactggcgcg taccatttca caaactaatg
     1561 ataatttaga tatgactaca gcatttaagt tattcaataa tcctcagtat gaaatttaca
     1621 gtaaactatt caaaagctgg tctggcacca tccatacatc aatgtatcaa cctgaaacat
     1681 taactgcata ttttacattg ggagaaaatg cgccaccaga aataatagat ttcaaatcat
     1741 ggttagatgg tcaagacctt aatatcactc actttactgg caatatagat accgacttaa
     1801 cttttgccaa caaataaata acaccgatat tagagaaatt atgcttccta atatcggtgc
     1861 ttacatcctt tatttattca actttgatat taccatcaat cgtataaaat tctaaaacat
     1921 tgtcgctttt cccaacttta ccattagtaa atgttttatt tttaactacg ctatcacccg
     1981 ttcccggatt tagctttagt atagtgtctt caggtttact atcatactta aaacgaatat
     2041 ctccttgttt agtagaagct tttgcatcta cttttgatgg catgttttta aattcaatgt
     2101 caccatgatc gtttaaaaat atggattgtt taatttgact cttgtcgatt tgtagcgtac
     2161 caataacaac tttaatattg ctatttttaa tgttactttt actaatataa gtagaactat
     2221 tatttgtctt tgaatctagt gcgtcaagat ttgagttttt aataactact tcaccgttaa
     2281 tgctttgaat acttgtgttc tctaaatcaa catcactgat tctaacactt cctgctccag
     2341 atgatagatt taaacgttta atcattttat caggcatttc aatcgttaac gttttcttat
     2401 tctcatgaaa aggattcata tcaattgcat atcctcttgt cttagacctt ttatcactaa
     2461 tcttcaacgt cttatctact atattaatta atatatcatt gtcaccatca tatttaactc
     2521 taaattgatt cccccgtttt atacgaagtt ctgtactatc caaatttata gatacattgt
     2581 caaaagcatc gtctttgaat gttttatcat attgtttagt accatatttg tttttatcat
     2641 agctgaacca aataatggtt gctgctgtaa aaaagacaac aaacactaaa agcccaataa
     2701 aaaagaattt cttcattttc taacgcttcc tttgacagtt ttaatatacc aaagtaaata
     2761 ttttaagatt aaacgataaa tgtattctaa aatcttaaat atgacaatga taaatactaa
     2821 tcctaatcct gaatatgata tggcaaaaaa tacattacta attgagtcca caattcctct
     2881 ggatattgct gatgctaata aaataattgg acaaaacagc agcaaaagtg atattaaaaa
     2941 taatgctaat acaaataatc caactataga tactggtata aggatgacaa aaatagatag
     3001 aatgcctaaa cttaatgaag cagcaacagc tcttgaaata ttttcaaatg ttggtttaga
     3061 ttcagcataa tcaataacac ttctagcttt tgtttcagct gcaacatctt gtgggtcttt
     3121 taacttcttt aaaatttccc cttcatcaac gccattaagt tcttgctcat aaaaataacg
     3181 ttcatattca tacatttttt gatctcttaa atgcctaggt agcgcatcaa gtgctgtttc
     3241 taattcattt aaataagtaa tcttgttcat ataggtcact catcccaata aaattattca
     3301 ttcgtttttt ataactttaa gacctaaagc tatttactaa aaaattcaat acgtttaatt
     3361 tctaaatctt gataaagtaa gtttaatttt aaatggttat ctctatcaat atactttacc
     3421 gatgagaaat cattgccaac ttttgttctt ttatatccga atccaagatt ttcaatcgcg
     3481 ttatttatcg gcgaaccaat tcccccatca aaattagtat gtatcttttc atctcttact
     3541 aatattatcc ccttaacgat gttatccttt tctctcactt taacggtaag atcagggtga
     3601 gctttactgt tataaaattt aaaacgatca aacttaattt ttttatttac ttcatagcct
     3661 tttttatcaa acttctcatt taatttaata tcgtttattt caagattaga caaatccaca
     3721 gttttcgtat gacgatattg atataaacca attaatacac caaaagaaca gattaaaaat
     3781 aatataaaca caacccagcg attttgaata cgcactgatg tcaccctttc tttgtactta
     3841 tttaaatata gcataaggtg cttcccgttt catccttaac aaaaattaac aaagaatttg
     3901 caattaattt atttcattac cttttattac tataaattgg tataatgttg taaatgctaa
     3961 aggaggtttt tacaatgaca aatttagaaa cttatttcaa aaatagccaa cccttaaatg
     4021 aatacattga tggaatgaag acaaatcaag ataccgttct cagtatctat caatcatttg
     4081 agttaccaga agatgattca cgcatcaaaa aaataaaaga gatgaattat tctaaagtat
     4141 tagtaattac tgaagactgg tgtggagatg caatgatgaa tctcccaatt ctaaaacaca
     4201 tcagtgaagc attaaattta gaagtacgcg tgttccatag agatgatgat acaaaactga
     4261 tcgatcaata tttaacaaat ggtaaatcac gtgcaattcc tatcttcgta ttcttgaatg
     4321 atcaattcga acaagaaaca gtttggggac caagagcaaa tgaagtccaa aaatttgtaa
     4381 ctgatgttcg tgctgataaa ttaccgagca aagatcatcc agactatgca gatttagaaa
     4441 aagaaacaca cttagttata tcaaaccgct ataaaactga cgcttcattt tggaaagcag
     4501 tttataattc catcctaaat aaattagaaa ataaat
//
LOCUS       DAXWFK010000177         4520 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.178, whole genome shotgun sequence.
ACCESSION   DAXWFK010000177 DAXWFK010000000
VERSION     DAXWFK010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4520
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.178"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..682
                     /locus_tag="ACGEXS_002339"
     CDS             <1..682
                     /locus_tag="ACGEXS_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="superantigen-like protein SSL7"
                     /protein_id="HFF8013323.1"
                     /translation="LAKATLALGLLTTGVITSEGQAVQAKEKQERVQHLYDIKDLHRY
                     YSSESFEFSNISGKVENYNGSNVVRFNQENQNHQLFLLGKDKEKYKEGIEGKDVFVVK
                     ELIDPNGRLSTVGGVTKKNNKSSETNTHLFVNKVYGGNLDASIDSFSINKEEVSLKEL
                     DFKIRQHLVKNYGLYKGTTKYGKITINLKDGEKQEIDLGDKLQFERMGDVLNSKDINK
                     IEVTLKQI"
     gene            1025..1723
                     /locus_tag="ACGEXS_002340"
     CDS             1025..1723
                     /locus_tag="ACGEXS_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL8"
                     /protein_id="HFF8013324.1"
                     /translation="MKFTVIAKAIFILGILTTSVMITENQSVNAKGKYEKMNRLYDTN
                     KLHQYYSGPSYELTNVSGQSQGYYDSNVLLFNQQNQKFQVFLLGKDENKYKEKTHGLD
                     VFAVPELVDLDGRIFSVSGVTKKNVKSIFESLRTPNLLVKKIDDKDGFSIDEFFFIQK
                     EEVSLKELDFKIRKLLIKKYKLYEGSADKGRIVINMKDENKYEIDLSDKLDFERMADV
                     INSEQIKNIEVNLK"
     gene            2103..2801
                     /locus_tag="ACGEXS_002341"
     CDS             2103..2801
                     /locus_tag="ACGEXS_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL9"
                     /protein_id="HFF8013325.1"
                     /translation="MKLTTIAKATLALGILTTGVFTAESQTGHAKVELDETQRKYYIN
                     MLHQYYSEESFEPTNISVKSEDYYGSNVLNFKQRNKAFKVFLLGDDKNKYKEKTHGLD
                     VFAVPELIDIKGGIYSVGGITKKNVRSVFGFVSNPSLQVKKVDAKNGFSINELFFIQK
                     EEVSLKELDFKIRKLLIEKYRLYKGTSDKGRIVINMKDEKKHEIDLSEKLSFERMFDV
                     MDSKQIKNIEVNLN"
     gene            3167..3850
                     /locus_tag="ACGEXS_002342"
     CDS             3167..3850
                     /locus_tag="ACGEXS_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL10"
                     /protein_id="HFF8013326.1"
                     /translation="MKFTELAKATLALGILTTGTLTTEVHSGHAKQNQKSVNKHDKEA
                     LYRYYTGKTMEMKNISALKHGKNNLRFKFRGIKIQVLLPGNDKSKFQQRSYEGLDVFF
                     VQEKRDKHDIFYTVGGVIQNNKTSGVVSAPILNISKEKGEDAFVKGYPYYIKKEKITL
                     KELDYKLRKHLIEKYGLYKTISKDGRVKISLKDGSFYNLDLRSKLKFKYMGEVIESKQ
                     IKDIEVNLK"
     gene            complement(3935..4036)
                     /locus_tag="ACGEXS_002343"
     CDS             complement(3935..4036)
                     /locus_tag="ACGEXS_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013327.1"
                     /translation="MENFDVGGGNFTFMEIGIGFMTHKPKRLNDDID"
     gene            4114..>4520
                     /locus_tag="ACGEXS_002344"
     CDS             4114..>4520
                     /locus_tag="ACGEXS_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HFF8013328.1"
                     /translation="MSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFY
                     RFLSEKAEQEYADALSGEDITYQEAWADEEYREDLKAELIDQVGYFIEPEDLFSAMIR
                     EIETQDFDIEHLATAIRKVETSTLGEESENDFIG"
BASE COUNT         1820 a          545 c          800 g         1355 t
ORIGIN      
        1 gttagctaaa gcaacattgg cattaggctt attaactact ggtgtgatta catcagaagg
       61 ccaagcagtt caagcaaaag aaaagcaaga gagagtacaa catttatatg atattaaaga
      121 cttacatcga tactactcat cagaaagttt tgaattcagt aatattagtg gtaaggttga
      181 aaattataac ggttctaacg ttgtacgctt taaccaagaa aatcaaaatc accaattatt
      241 cttattaggt aaagataaag agaaatataa agaaggcatt gaaggcaaag atgtctttgt
      301 ggtaaaagaa ttaattgatc caaacggtag attatctact gttggtggtg tgactaagaa
      361 aaataacaaa tcttctgaaa ctaatacaca tttatttgtt aataaagtgt atggcggaaa
      421 tttagatgca tcaattgact cattttcaat taataaagaa gaagtttcac tgaaagaact
      481 tgatttcaaa attagacaac atttagttaa aaattatggt ttatataaag gtacgactaa
      541 atacggtaag atcactatca atttgaaaga tggagaaaag caagaaattg atttaggtga
      601 taaattgcaa ttcgagcgca tgggtgatgt gttgaatagt aaggatatta ataagattga
      661 agtgactttg aaacaaattt aaagtaagta atcaatgact ctaaagtaat aaatttgaag
      721 cagcttaacg atgaaatgtt gaatagatac gtacacctta cattaaggag cgtatttaaa
      781 acaaccttgt cgttaggctt tttttacgtt ttataacgca ggttatgagt gagcatacta
      841 aaaatttaca ttgctcttga aagtgatgcc cattgaatat taattagctc ttcattaaca
      901 atgatttaag tttaattaaa cgagtgttaa tgtcagtctg tctcaatgcc ctttataata
      961 aaggtgtatt attcaaatta cgtaataaaa gcaatccaat atattaagat tggagcacat
     1021 gaatatgaaa tttacagtga tagctaaagc gatatttata ttaggaatat taacaacaag
     1081 tgtaatgata acagaaaatc aatcggttaa tgcaaaagga aagtatgaaa aaatgaaccg
     1141 tttatatgat acaaacaagt tacatcaata ctattcagga cctagttatg agttaacaaa
     1201 tgttagtggc caaagtcaag gttattatga ctctaacgtt ttgcttttta accaacaaaa
     1261 tcaaaagttc caagtgtttt tattgggaaa agatgaaaat aaatacaaag aaaaaacaca
     1321 tggtttagat gtctttgcgg taccagaatt agtagattta gatggaagaa tatttagtgt
     1381 tagtggtgta acaaagaaaa acgtaaaatc aatatttgag tctctaagaa cgccgaactt
     1441 actagttaaa aaaatagacg ataaagacgg tttttctatt gatgaatttt tctttattca
     1501 aaaggaagaa gtgtcattga aggaacttga ttttaaaata agaaaactgt tgattaaaaa
     1561 atacaaactg tatgaagggt cagctgataa aggtagaatt gttattaata tgaaagatga
     1621 aaataagtat gaaattgatt taagtgataa attagatttc gagcgtatgg cagatgtcat
     1681 taatagtgaa caaattaaaa acatcgaagt gaatttgaaa taatcaatga tatatataga
     1741 atgaaagctt aagaagcggt ttaataatcc catgtttaat gattttgata cgtgttttta
     1801 taataaaaac atatcgaaca ttgactacgt tattaagctg cttttttgta cactttataa
     1861 ccaatagctt aagatttaaa actaatcgga aagaacaatg attcaccaaa aaaatattta
     1921 tgttgctatt aaaaatcagt taatacgaat gttaaaatac gtttgatttt cattaataat
     1981 gattcaagtt tatttaaatg agcgttaatg tcagtctgtt ttgatgcacc ttataataaa
     2041 gacagatagt tcaaattacg taataataac aatccaatac attaagattg gagcaaaaaa
     2101 atatgaaatt aacaacgata gctaaagcaa cattagcatt aggaatatta actacaggtg
     2161 tgtttacagc agaaagtcaa actggtcacg cgaaagtaga acttgatgag acacaacgca
     2221 aatattatat caatatgcta catcaatact attctgaaga aagttttgaa ccaacaaaca
     2281 ttagtgttaa aagcgaagat tactatggct ctaacgtttt aaactttaaa caacgaaata
     2341 aagcttttaa agtattttta cttggtgacg ataaaaataa atataaagaa aaaacacatg
     2401 gccttgatgt ctttgcagta cctgaattaa tagatataaa aggtggcata tatagcgttg
     2461 gcggtataac aaagaaaaat gtgagatcag tgtttggatt tgtaagtaat ccaagtctac
     2521 aagttaaaaa agttgatgct aaaaatggct tttcgataaa cgagttgttt tttattcaaa
     2581 aggaagaagt atcattgaag gaactggact ttaaaataag aaaactctta atcgaaaaat
     2641 atagattgta taaaggaacg tctgataaag gtagaattgt tatcaatatg aaagacgaaa
     2701 agaagcatga aattgattta agtgaaaaat taagttttga acgtatgttt gatgtaatgg
     2761 atagtaagca aattaaaaat attgaagtga atttgaatta gttcgagtta atagcataat
     2821 agcttaagaa gcggcttaac gacaaaatgt gaattggcat ttgtgtcctt atataaggaa
     2881 ctgtgttaaa tacattactg ttgttaagtt gtttttgtaa ttcaaagagc agaacagagt
     2941 aacatcatca gttgtagtaa acgataatcc ggtaaaacaa ctaaatgaaa taatgaaagt
     3001 catttaacct gaacattaaa atatatttgt ttttcattaa gaataattca agtatattta
     3061 aatcgaggtt aattatcgta tgaaacgatg cacgttataa taaaaatgta tgattcaaat
     3121 tacgtaatga aaacaatcca atatattaag attggagcaa ataaatatga aatttacaga
     3181 attagcaaaa gcgacattag ctttaggaat tttaacaaca ggaactttaa caacagaagt
     3241 tcattcaggt catgcaaaac aaaatcaaaa gtcagtaaat aaacatgaca aggaagcatt
     3301 ataccgatac tacactggaa agactatgga aatgaaaaat attagtgctt tgaaacatgg
     3361 taaaaacaac ttacgtttta agtttagagg tattaagatt caagttttac tgcctggaaa
     3421 tgataaaagt aaatttcaac agcgtagtta tgaggggtta gatgttttct ttgttcaaga
     3481 aaaaagagat aagcacgata tattttatac tgttggtggt gtaatacaga ataataaaac
     3541 atctggagtt gtcagtgcac caatattaaa tatttcaaaa gaaaagggtg aagatgcttt
     3601 tgtgaaaggt tacccttatt acattaaaaa agaaaaaata acactaaaag aactggatta
     3661 taagttgaga aagcatctaa ttgaaaaata cggactttat aaaacaatct caaaagatgg
     3721 tagggtcaaa attagcttga aagatggcag tttttataac cttgatttaa gatctaaatt
     3781 aaaatttaaa tatatggggg aagtcataga aagcaaacaa attaaagata ttgaagttaa
     3841 cttaaagtaa atcattacga ataattaaaa gtaattgaag cggcttaacg gtgaaatgta
     3901 aattggtgcg catagcttat acaaaaagga tgcatcaatc gatatcgtcg ttaagccgtt
     3961 ttggtttgtg tgtcatgaat cctatcccaa tctccataaa ggtaaaattt ccaccaccaa
     4021 catcaaaatt ctccacatcg caacataacc aaatgttata ataaatctat tacacaaaga
     4081 gataaattac ttattcaaag gcggaggaat cacatgtcta ttactgaaaa acaacgtcag
     4141 caacaagctg aattacataa aaaattatgg tcgattgcga atgatttaag agggaatatg
     4201 gatgcgagtg aattccgtaa ttacatttta ggcttgattt tctatcgctt cttatctgaa
     4261 aaagcggaac aagaatatgc agatgccttg tcaggtgaag acatcacgta tcaagaagca
     4321 tgggcagacg aagaataccg tgaagactta aaagcagaat taattgacca agtcggttac
     4381 ttcattgagc cagaagattt attcagtgcg atgattcgtg aaattgaaac gcaagatttc
     4441 gatatcgaac acctggcgac ggcaattcgt aaagttgaaa catcaacatt aggtgaagaa
     4501 agtgaaaatg actttatcgg
//
LOCUS       DAXWFK010000178         4441 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.179, whole genome shotgun sequence.
ACCESSION   DAXWFK010000178 DAXWFK010000000
VERSION     DAXWFK010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4441
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.179"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            40..981
                     /gene="pde2"
                     /locus_tag="ACGEXS_002345"
     CDS             40..981
                     /gene="pde2"
                     /locus_tag="ACGEXS_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pApA hydrolase Pde2"
                     /protein_id="HFF8013329.1"
                     /translation="MISTLNEIMKCIEDNDTIIIHRHVRPDPDAYGSQLGLKYYIQQK
                     FPQKQVFAVGEAESSLSFIGELDNIDDKTYQDALVIVCDTANAPRIDDERYSTGRKLI
                     KIDHHPAVDQYGDINLVNTNASSTSEIIYDLISHFNDEAIVNKDIASVLYLGIVGDTG
                     RFLFNNTSEHTMEIAGKLIGHDIDHNALLNKMMEKDPKMLPFQGYVLQHFELMDDGFC
                     QVKITEDVLEQFGIQPNEASQFVNTIADIKGLKIWVFAVDEGNEIRCRLRSKGQLIIN
                     DIAQDFGGGGHPNASGVSVDSWDEFEQLATALRTKLN"
     gene            1002..4199
                     /locus_tag="ACGEXS_002346"
     CDS             1002..4199
                     /locus_tag="ACGEXS_002346"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFF8013330.1"
                     /translation="MVAYLNIHTAYDLLNSSLKIEDAVRLAVSENVDALAITDTNVLY
                     GFPKFYDACIANNIKPIFGMTIYVTNGLNTVETVVLAKNNDGLKDLYQLSSEIKMNAL
                     EHVSFELLKRFSNNMIIIFKKVGDQHRDIVQVFETHNDTYMDHLSISIQGRKHVWIQN
                     VCYQTRQDADTISALAAIRDNTKLDLIHDQEDFGAHFLTEKEINQLDINQEYLTQVDV
                     IAQKCDAELKYHQSLLPQYETPNDESAKKYLWRVLVTQLKKLELNYDVYLERLKYEYK
                     VITNMGFEDYFLIVSDLIHYAKTNDVMVGPGRGSSAGSLVSYLLGITTIDPIKFNLLF
                     ERFLNPERVTMPDIDIDFEDTRRERVIQYVQEKYGELHVSGIVTFGHLLARAVARDVG
                     RIMGFDEVTLNEISSLIPHKLGITLDEAYQIDDFKKFVHRNHRHERWFSICKKLEGLP
                     RHTSTHAAGIIINDHPLYEYAPLTKGDTGLLTQWTMTEAERIGLLKIDFLGLRNLSII
                     HQILTQVKKDLGINIDIEKIPFDDQKVFELLSQGDTTGIFQLESDGVRSVLKKLKPEH
                     FEDIVAVTSLYRPGPMEEIPTYITRRHDPSKVQYLHPHLEPILKNTYGVIIYQEQIMQ
                     IASTFANFSYGEADILRRAMSKKNRAVLESERQHFIEGAKQNGYHEDISKQIFDLILK
                     FADYGFPRAHAVSYSKIAYIMSFLKVHYPNYFYANILSNVIGSEKKTAQMIEEAKKQG
                     ITILPPNINESHWFYKPSQEGIYLSIGTIKGVGYQSVKVIVDERYQNGKFKDFFDFAR
                     RIPKRVKTRKLLEALILVGAFDAFGKTRSTLLQAIDQVLDGDLNIEQDGFLFDILTPK
                     QMYEDKEELPDALISQYEKEYLGFYVSQHPVDKKFVAKQYLTIFKLSNAQNYKPILVQ
                     FDKVKQIRTKNGQNMAFVTLNDGIETLDGVIFPNQFKKYEELLSHNDLFIVSGKFDHR
                     KQQRQLIINEIQTLATFEEQKLAFAKQIIIRNKSQIDMFEEMIKATKENANDVVLSFY
                     DETIKQMTTLGYINQKDSMFNNFIQSFNPSDIRLI"
BASE COUNT         1592 a          602 c          814 g         1433 t
ORIGIN      
        1 cttaaattaa gcaacttttc taaaaaaggg agtatgaaaa tgattagtac tttgaatgaa
       61 attatgaaat gtatcgaaga caacgataca attattatac acagacatgt tagaccagat
      121 cctgatgcat atggttcaca acttggttta aaatactata ttcagcagaa atttccgcaa
      181 aagcaagtat ttgcagtggg tgaagcggaa tcatcattaa gttttattgg agagttagat
      241 aacattgatg ataaaacata tcaagatgcg cttgtaattg tatgtgatac tgccaatgct
      301 ccacgaattg acgatgaacg ttatagtaca ggtcgtaaac ttattaaaat agatcatcat
      361 cctgcagttg atcagtatgg tgatattaat ttagttaata cgaacgcgtc atctacaagt
      421 gaaatcattt acgatttaat ctcacatttt aatgatgaag caattgttaa taaagatatc
      481 gcgagcgttt tatatcttgg tatcgtcggt gatactgggc gattcctttt taacaatacc
      541 tcagaacata ctatggaaat tgctggaaag ttaattgggc atgatattga tcataatgcg
      601 cttttaaata aaatgatgga gaaggaccca aaaatgttgc cgtttcaagg ttatgtttta
      661 caacatttcg aacttatgga tgatggattc tgccaagtta aaataactga agatgtattg
      721 gagcaattcg gtattcagcc aaatgaagca tctcagtttg ttaatacaat tgctgacatc
      781 aaaggcttga aaatatgggt atttgcagtc gatgaaggta atgaaatcag atgtcgatta
      841 cgttctaaag ggcaattgat tattaatgat attgcgcaag attttggtgg cggtggtcat
      901 ccgaatgcgt caggagtttc agtggacagc tgggatgaat ttgagcaact tgctacagct
      961 ttacgcacaa aacttaacta atagaaaggg gccattcaat catggtggca tatttaaata
     1021 ttcatacggc ttatgatttg ttaaattcaa gcttaaaaat agaagatgcc gtaagacttg
     1081 ctgtgtctga aaatgttgat gcacttgcca taactgacac caatgtattg tatggttttc
     1141 ctaaatttta tgatgcatgt atagcaaata acattaaacc gatttttggt atgacaatat
     1201 atgtgacaaa tggattaaat acagtcgaaa cagttgttct agctaaaaat aatgatggat
     1261 taaaagattt gtatcaacta tcatcggaaa taaaaatgaa tgcattagaa catgtgtcgt
     1321 ttgaattatt aaaacgattt tctaacaata tgattatcat ttttaaaaaa gtcggtgatc
     1381 aacatcgtga tattgtacaa gtgtttgaaa cccataatga cacatatatg gaccacctta
     1441 gtatttcgat tcaaggtaga aaacatgttt ggattcaaaa tgtttgttac caaacacgtc
     1501 aagatgccga tacgatttct gcattagcag ctattagaga caatacaaaa ttagacttaa
     1561 ttcatgatca agaagatttt ggtgcacatt ttttaactga aaaggaaatt aatcaattag
     1621 atattaacca agaatattta acgcaggttg atgttatagc tcaaaagtgt gatgcagaat
     1681 taaaatatca tcaatctcta cttcctcaat atgagacacc taatgatgaa tcagctaaaa
     1741 aatatttgtg gcgtgtctta gttacacaat tgaaaaaatt agaacttaat tatgacgtct
     1801 atttagagcg attgaaatat gagtataaag ttattactaa tatgggtttt gaagattatt
     1861 tcttaatagt aagtgattta atccattatg cgaaaacgaa tgatgtgatg gtaggtcctg
     1921 gtcgtggttc ttcagctggc tcactggtca gttatttatt gggaattaca acgattgatc
     1981 ctattaaatt caatctatta tttgaacgtt ttttaaaccc agaacgtgta acaatgcctg
     2041 atattgatat tgactttgaa gatacacgcc gagaaagggt cattcagtac gtccaagaaa
     2101 aatatggcga gctacatgta tctggaattg tgactttcgg tcatctgctt gcaagagcag
     2161 ttgctagaga tgttggaaga attatggggt ttgatgaagt tacattaaat gaaatttcaa
     2221 gtttaatccc acataaatta ggaattacac ttgatgaagc atatcaaatt gacgatttta
     2281 aaaagtttgt acatcgaaac catcgacatg aacgctggtt cagtatttgt aaaaagttag
     2341 aaggtttacc aagacataca tctacacatg cggcaggaat tattattaat gaccatccat
     2401 tatatgaata tgccccttta acgaaagggg atacaggatt attaacgcaa tggacaatga
     2461 ctgaagccga acgtattggg ttattaaaaa tagattttct agggttgaga aacttatcga
     2521 ttattcatca aatcttaaca caagtcaaaa aagatttagg tattaatatt gatatcgaaa
     2581 agattccgtt tgatgatcaa aaagtgtttg aattgttgtc gcaaggagat acgactggca
     2641 tattccaatt agagtctgac ggtgtaagaa gtgtattaaa aaaattaaag ccggaacact
     2701 ttgaagatat tgttgctgta acttctttgt atagaccagg tccaatggaa gaaattccaa
     2761 cttacattac aagaagacat gatccaagca aagttcaata tttacatccg catttagaac
     2821 ctatattaaa aaatacttac ggtgttatta tttatcaaga gcaaattatg caaatagcga
     2881 gcacatttgc aaacttcagt tatggtgaag cggatatttt aagaagagca atgagtaaaa
     2941 aaaatagagc tgttcttgaa agtgagcgtc aacattttat agaaggtgca aagcaaaatg
     3001 gttatcacga agacattagt aagcaaatat ttgatttgat tctgaaattt gctgattatg
     3061 gttttcctag agcacatgct gtcagctatt ctaaaattgc atacattatg agctttttaa
     3121 aagtccatta tccaaattat ttttacgcaa atattttaag taatgttatt ggaagtgaga
     3181 agaaaactgc tcaaatgata gaagaagcaa aaaaacaagg tatcactata ttgccaccga
     3241 atattaacga aagtcattgg ttttataaac cttcccaaga aggcatttat ttatcaattg
     3301 gtacaattaa aggtgttggt tatcaaagtg tgaaagtgat tgttgatgaa cgttatcaga
     3361 acggcaaatt taaagatttc tttgattttg ctagacgtat accgaagaga gtcaaaacga
     3421 gaaagttact tgaagcactg attttagtgg gagcgtttga tgcttttggt aaaacacgtt
     3481 caacgttgtt gcaagctatt gatcaagtgt tggatggcga tttaaacatt gaacaagatg
     3541 gttttttatt tgatatttta acgccaaaac agatgtatga agataaagaa gaattgcctg
     3601 atgcacttat tagtcagtac gaaaaagaat atttaggatt ttatgtttcg caacacccag
     3661 tagataaaaa gtttgttgcc aaacaatatt taacgatatt taaattgagt aacgcgcaga
     3721 attataaacc tatattagta cagtttgata aagttaaaca aattcgaact aaaaatggtc
     3781 aaaatatggc attcgtcaca ttaaatgatg gcattgaaac tttagatggt gtgattttcc
     3841 ctaatcagtt taaaaagtac gaagagttgt tatcacataa tgacttgttt atagttagcg
     3901 ggaaatttga ccatagaaag caacaacgtc aactaattat aaatgagatt cagacattag
     3961 ccacttttga agaacaaaaa ttagcatttg ccaaacaaat tataattaga aataaatcac
     4021 aaatagatat gtttgaagag atgattaaag ctacgaaaga gaatgctaat gatgttgtgt
     4081 tatcctttta tgatgaaacg attaaacaaa tgactacttt aggctatatt aatcaaaaag
     4141 atagtatgtt taataatttt atacaatcct ttaaccctag tgatattagg cttatataac
     4201 ttttatcaac taataaatta tgatatagta aactgatggt tagatatttt ttaaccatca
     4261 gtttgcgttt atattagttt tttatgctta ttatttttat gagtttcact ttacattatt
     4321 gattaatcca taagaataat tagcacaaaa agcagtatac ataaattgag taaagaattt
     4381 tgtcgatata tcgtgcggaa aaataataac tttttgatat attggttttt gctatccatt
     4441 a
//
LOCUS       DAXWFK010000179         4406 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.180, whole genome shotgun sequence.
ACCESSION   DAXWFK010000179 DAXWFK010000000
VERSION     DAXWFK010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.180"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            66..749
                     /locus_tag="ACGEXS_002347"
     CDS             66..749
                     /locus_tag="ACGEXS_002347"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFF8013331.1"
                     /translation="MEWILFDKDGTLIEFDRSWEKIGVRFVQSLLETFPVHNKEAALR
                     QLGVIKESIDPKSVMGSGSLQQIIQAFNDVTGQDTTDWSKSTSQKLVDERIPEINWVE
                     GVKEALIDLKAKGYQLGIVTSDTKKGVEQFLAHTNATSLFDLIISTEADAYEKPNPKV
                     LSPLFEQYNVDPQKVAIVGDTANDMKTASNANLGMAIGVLTGIATKEELHEADIILNS
                     AADILEALN"
     gene            complement(865..1527)
                     /locus_tag="ACGEXS_002348"
     CDS             complement(865..1527)
                     /locus_tag="ACGEXS_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFF8013332.1"
                     /translation="MNNYGIPQNAIITIAGTVGVGKSTLTQALADKLNFKTSFENVEH
                     NPYLDKFYSDFERWSFHLQIYFLAERFKEQKRMFEYGGGFVQDRSIYEDVDIFAKMHE
                     EEGTMSKEDFKTYSDLFNAMVMTPYFPKPDVMIYLECNYDEVIDRIIERGREMEINTD
                     PEYWKKLFKRYDDWINSFNACPVVRININEYDIHKDPESLNPMINKIARIIQTYRQVD
                     TR"
     gene            complement(1520..2137)
                     /locus_tag="ACGEXS_002349"
     CDS             complement(1520..2137)
                     /locus_tag="ACGEXS_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFF8013333.1"
                     /translation="MNKPFIAIEGPIGVGKSSLAHKLSQTLDFYEEKEIITENPFLSD
                     FYEDISKWSFQTEMFFLCNRYKQFQDVTQLNQGVVSDYHIHKNKIFAKNTLSSVEFQK
                     FSKIYDILTEDMIMPNMIIFLDADLDVLKSRIAKRNRSFEHQIEDEYLLKLKKDYREY
                     YESLQSNGSNVVLIDTTSIDFLKNEQDYEDILHIILPMIGDITNE"
     gene            2204..2674
                     /gene="tadA"
                     /locus_tag="ACGEXS_002350"
     CDS             2204..2674
                     /gene="tadA"
                     /locus_tag="ACGEXS_002350"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFF8013334.1"
                     /translation="MTNDIYFMTLAIEEAKKAAQLGEVPIGAIITKDDEVIARAHNLR
                     ETLQQPTAHAEHIAIERAAKVLGSWRLEGCTLYVTLEPCVMCAGTIVMSRIPRVVYGA
                     DDPKGGCSGSLMNLLQQSNFNHRAIVDKGVLKEACSTLLTTFFKNLRANKKSTN"
     gene            2821..3690
                     /locus_tag="ACGEXS_002351"
     CDS             2821..3690
                     /locus_tag="ACGEXS_002351"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFF8013335.1"
                     /translation="MIKLIATDMDGTLLNGAHEISQPNIDAIKYAQEQGITVVIATGR
                     AFYEAQAPVADTDLTVPYICLNGAEVRDETFNVMSTSHLNKSLVHKITNVLKDAGIYY
                     QVYTSRAIYTEDPQRDLDIYIDIAERAGQHANVERIKNGIQRRIDNGTLKVVDNYDAI
                     ENIPGELIMKILAFDGNLEKIDKASKILAESPNLAISSSSRGNIEITHSDAQKGIALE
                     TIAERLGIEMKEVMSIGDNLNDLSMLEKVGYPVAMENGAEEVKKIAKYVTDTNENSGV
                     GKAIMKLLREQQV"
     gene            3711..4277
                     /locus_tag="ACGEXS_002352"
     CDS             3711..4277
                     /locus_tag="ACGEXS_002352"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFF8013336.1"
                     /translation="MKGLIIIGSAQVNSHTSALARYLTEHFKTHDIEAEIFDLAEKPL
                     NQLDFSGTTPSIDEIKQNMKDLKEKAMAADFLILGTPNYHGSYSGILKNALDHLNMDY
                     FKMKPVGLIGNSGGIVSSEPLSHLRVIVRSLLGIAVPTQIATHDSDFAKNEDGSYYLN
                     DSEFQLRARLFVDQIVSFVNNSPYEHLK"
BASE COUNT         1610 a          624 c          766 g         1406 t
ORIGIN      
        1 caggggataa cgaatatcat agaatcagca taatacgaaa taaataaata ggagtattga
       61 tatcaatgga atggatatta tttgataaag atggtacgtt aattgaattt gatagaagtt
      121 gggaaaaaat aggggtacga tttgtacaat cattgcttga gactttccca gtacataata
      181 aagaagctgc tttaagacaa ctcggtgtca ttaaagaatc tattgatcca aaatcagtga
      241 tgggttcagg atctttacaa caaattatcc aggcatttaa tgatgtgacg ggacaagata
      301 caaccgactg gtccaagtca acaagtcaaa agctggtaga tgaacgtatt cctgaaatta
      361 attgggtaga aggtgttaaa gaagcactta tcgatttgaa agcaaaaggc tatcaacttg
      421 gtattgttac gagtgatact aaaaaaggtg tagaacaatt tttagcacat accaatgcta
      481 cctcgttgtt cgatttgatc atttctaccg aagcggatgc ctatgagaag ccaaatccta
      541 aagtattatc gcctttattt gagcaatata atgtagatcc tcagaaagta gctatagtag
      601 gagacactgc taatgatatg aagacagcaa gtaatgcaaa tttaggtatg gcaataggtg
      661 tattaacagg tattgcaaca aaagaagaat tacatgaagc tgatattatt ttaaatagtg
      721 cggcagatat tttagaagct ttaaattaaa aagaaagaca tagtgtatat gtgaattaat
      781 gtattcgata gaattgataa aataatcgat aatatctatg gaatatacat tgaatataaa
      841 catatacgct atgtctttag tcttttatcg tgtatctact tgtcgatatg tttgaataat
      901 tcgagcaatt ttgtttatca taggatttaa agattcgggg tccttatgga tatcatattc
      961 attaatattg atacgtacaa ctggacatgc attaaagcta ttaatccaat cgtcatagcg
     1021 tttaaatagc tttttccagt attcagggtc tgtattaatt tccatttcgc gaccacgttc
     1081 aataatacga tcaatgacct catcatagtt acattctaaa taaatcatta catcaggttt
     1141 aggaaaataa ggtgtcatga ccatggcatt aaataagtct gaatatgttt tgaaatcttc
     1201 tttactcatt gtgccttctt cttcatgcat ttttgcaaaa atatcaacat cttcataaat
     1261 tgatcgatct tggacaaagc caccaccata ttcaaacata cgcttttgtt ctttaaaacg
     1321 ttcagctaag aagtaaattt gcaaatggaa actccatcgt tcaaaatcgc tgtaaaattt
     1381 atctaaatat ggattatgtt cgacattttc aaaagacgtt ttaaagttta atttatctgc
     1441 aagtgcttgc gttagtgttg attttccaac accaactgta cctgcaatgg ttataatggc
     1501 attttgtgga ataccgtaat tattcattgg taatatctcc tatcataggt aatataatat
     1561 gtaatatatc ttcgtaatct tgttcatttt taagaaaatc aatagaagtt gtatcgatta
     1621 aaactacatt tgaaccatta ctttgtaagg actcataata ctcacgataa tcttttttta
     1681 actttaacag atattcatct tctatttgat gctcaaaact acggttacgt ttagcaattc
     1741 tagattttaa cacatcaagg tctgcatcta aaaagataat catattcggc ataatcatat
     1801 cttcagttaa aatatcataa attttactga atttctgaaa ttcaacagaa ctcaaagtat
     1861 ttttagcaaa tatcttattt ttatgtatat gataatcact aactacacct tgatttagtt
     1921 gtgttacatc ttgaaattgc ttatatctat tgcataaaaa gaacatttca gtttgaaaac
     1981 tccatttaga gatatcttca taaaagtctg ataaaaatgg attttctgtg atgatttctt
     2041 tttcttcata aaaatctaaa gtttgactta atttgtgtgc aagtgaagat ttacctacgc
     2101 caataggacc ttcaattgct ataaaaggtt tgttcatatt cacatctcca aatgttaaaa
     2161 tagacattga gtattgtacc ataagtgagg tatctggaca taaatgacaa atgatatata
     2221 ttttatgaca ttagcgattg aagaagctaa aaaagcagct caactaggcg aagtacctat
     2281 aggtgctatc atcactaaag atgatgaagt tatcgctaga gcacataatt taagagaaac
     2341 actacaacaa ccaacggcgc atgctgaaca tattgcaatt gaacgtgcag ccaaagtgtt
     2401 aggtagttgg cgtttagaag gttgcacatt atatgtaacc ttagaaccat gtgtcatgtg
     2461 cgcaggaaca attgtaatga gtcgcattcc aagagtcgtc tatggcgcag atgatcctaa
     2521 aggtggttgt agtggcagtt taatgaattt attgcaacaa tctaatttta atcatcgtgc
     2581 aattgttgat aaaggtgtac ttaaagaagc atgtagcaca ttattaacaa cattttttaa
     2641 aaacttaaga gccaataaga aatccaccaa ttagacgata gtacatacta attaaaattt
     2701 gttagaatta cattatatga taaattaatg acaatataaa tgttaataga aaagtatgtg
     2761 cgctgagtat atattcttat ttaagaacaa gattttaaat ttacgaaacg aggtaacata
     2821 atgataaaac taatagccac tgatatggat ggcacgctac ttaatggagc acatgaaatt
     2881 tctcaaccta atattgatgc gattaaatac gctcaagaac aagggataac ggttgttatc
     2941 gcgacaggtc gagcatttta tgaagcacaa gcaccagttg ctgacacaga tttaacagta
     3001 ccatatattt gtttgaatgg tgctgaagta cgtgatgaaa ctttcaatgt aatgagcact
     3061 tcacacctta ataaatcgtt agtacacaaa attacaaatg ttttaaaaga tgcaggtatt
     3121 tattatcaag tatacacgag tcgtgcgatt tatactgaag atccacaaag agatttagac
     3181 atttacatag atattgctga gcgtgcaggt caacatgcaa acgttgagcg tattaaaaat
     3241 ggtattcaaa gacgcataga taatggtacg ttgaaagttg ttgataatta tgatgctatt
     3301 gaaaacatac ctggtgaatt aattatgaaa atattagcat ttgatggaaa tttagaaaaa
     3361 attgacaaag ctagtaaaat tttagctgaa tctccgaatt tagctatatc atcatcttcg
     3421 agaggaaata tagaaataac gcattcagat gcacaaaaag gtattgcgct agaaacaatt
     3481 gccgaaagat tagggattga aatgaaagaa gtcatgtcaa taggtgacaa tttaaatgac
     3541 ttatcaatgt tagagaaagt tggctatcca gttgcgatgg aaaatggtgc agaagaagtt
     3601 aaaaaaatag cgaaatatgt cacagatacg aatgaaaata gtggtgttgg aaaagctatt
     3661 atgaaattat tacgtgaaca acaagtttaa taaaaaaaga ggggtcaaat atgaaaggat
     3721 taattattat tggcagtgca caagtgaatt cacatacaag tgcactagca agatacttaa
     3781 ctgagcattt taaaacacat gatattgaag cggaaatatt cgatttagca gaaaaaccgt
     3841 taaatcaatt agatttttca ggaacaacac cgtctattga tgaaatcaaa caaaatatga
     3901 aagatttaaa agagaaagca atggcggcgg actttttaat attaggaacg ccaaactatc
     3961 atggttcata ttctggaata ttgaaaaatg cattagatca tctaaatatg gattatttta
     4021 aaatgaaacc tgtaggctta ataggaaata gtggtggtat tgttagttca gagccattgt
     4081 cacatttaag agtaatcgtc agaagtttac taggcattgc tgtaccaact caaatagcaa
     4141 cacatgattc tgattttgct aaaaatgaag atggttcata ttacttaaat gatagtgaat
     4201 tccaattacg agcaagatta tttgtcgatc aaattgtatc ttttgtgaat aatagtccat
     4261 atgaacattt aaaataatat taaaaaatat gtaaatatac attaaaaata ggtatgtagc
     4321 ttaaggtatt tcattgagaa tgcttttaag tcacatgcct attttttgtt ctaatgcacc
     4381 aatagtaagc ctttgatatt actgtt
//
LOCUS       DAXWFK010000180         4296 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.181, whole genome shotgun sequence.
ACCESSION   DAXWFK010000180 DAXWFK010000000
VERSION     DAXWFK010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.181"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            42..503
                     /gene="tsaE"
                     /locus_tag="ACGEXS_002353"
     CDS             42..503
                     /gene="tsaE"
                     /locus_tag="ACGEXS_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFF8013337.1"
                     /translation="MIKINNLDEMNQFAIFLVEQLKSGDLILLNGDLGAGKTTLTQFI
                     GKALGVRRTINSPTFNIIKSYRGKNLKLHHMDCYRLEDSDEDLGFDEFFEDQAITVIE
                     WSQFIKDLLPATHLSINISTISENTRQIELFAQGEHYEQIKEAIIHEFAAH"
     gene            484..1146
                     /gene="tsaB"
                     /locus_tag="ACGEXS_002354"
     CDS             484..1146
                     /gene="tsaB"
                     /locus_tag="ACGEXS_002354"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFF8013338.1"
                     /translation="MNSLLIDTSNQPLSVALMQNDEVLAEITTDSKQNHSVQLMPAIS
                     QLFEQSKIAKQQLDAIIVAEGPGSYTGLRIGVTVAKTLAYALDVKLYGVSSLKALAAT
                     IDHTDKLLVPVFDARRQAVYTGIFQWQNGQLVTILEDQYMSIEALRAFLEDLNQPFIY
                     IGKDTVKLQDELQGEVIAQLPNASVMYQLLDKPSDVHTFTPKYHKLAEAERNWINSQK
                     NN"
     gene            1119..1583
                     /gene="rimI"
                     /locus_tag="ACGEXS_002355"
     CDS             1119..1583
                     /gene="rimI"
                     /locus_tag="ACGEXS_002355"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFF8013339.1"
                     /translation="MDQQSKEQLNIREMTKEDVPQVFDLERRSFNDSSWTIDAFYHEI
                     EQNNFAKYFVLEFEQQIIGYLGLWIVIDQAQITTVAIDDNYRGYGLGQMLLKYGINYA
                     SHTCDVMSLEVRVNNKVAQHVYENLGFQYGGKRKNYYGEGEDAMVMWVNLND"
     gene            1576..2601
                     /gene="tsaD"
                     /locus_tag="ACGEXS_002356"
     CDS             1576..2601
                     /gene="tsaD"
                     /locus_tag="ACGEXS_002356"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFF8013340.1"
                     /translation="MTKDILILAVETSCDETSVSVIKNGRDILSNTVLSQIESHKRFG
                     GVVPEVASRHHVEGITATINEALGDADVSIEDIDAIAVTEGPGLIGALLIGVNAAKAL
                     AFAYDKPLIPVHHIAGHIYANHIEEPLTFPLIALIVSGGHTELVYMKDHLSFEVIGET
                     RDDAVGEAYDKVARTIGLNYPGGPQVDRLAAEGEDTYSFPRVWLDKDSYDFSFSGLKS
                     AVINQLHNQRQKNIPIIEANVATSFQNSVVEVLTFKAIQACKEYGVQRLIVAGGVASN
                     KGLRQSLADQCKVNDIQLTIPSPKLCTDNAAMIGVAGHYLYQQGRFADLALNGHSNID
                     LEEYSAE"
     gene            complement(2703..>4296)
                     /locus_tag="ACGEXS_002357"
     CDS             complement(2703..>4296)
                     /locus_tag="ACGEXS_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MutS family DNA mismatch repair protein"
                     /protein_id="HFF8013341.1"
                     /translation="FLIFVIAIILLTSVIGIVGRYMSRQRLLKSMETLWQTISPLEAF
                     IRPNSHFDYEYKLYKEKFESHSLVDDKTWSDLNMNAIFHKMNYNLTAIGEMKLYACLR
                     GMLSITNKSLLSLFNDNAEFRKNVTYHLALIGKTVYPTFPDQITPVKRQNILFLCPFL
                     PVISFAVIFINSQVGILLFLMSCLFNIILSATLKRTYEDDLKSIFYASNVLKQGYTIS
                     KIKHAPQPEVNFKQFRTARHLTSVLAEVNDEDIGAMVIKLVKLIFMLDYVLFHSIQKS
                     YTTHMNELKNCFDYIAELDNHYALAMYRRTLECYTEPQIDDSNDGIVFSELTHPLIAD
                     AVANDFSLSQNILLTGSNASGKSTFMKSIAINIILASAIQTVTASKFVYQPGIVFTSM
                     ANADDVLSGDSYFMAELKSIKRIVEIPDNQKIYCFIDEIFKGTNTTERIAASESVLSF
                     LHEKSNFRVIAATHDIELAELLKQRYENYHFNEVIENNNIHFDYKIKPGKANTRNAIE
                     LLKITSFPAKIYERAKDNVPKI"
BASE COUNT         1465 a          615 c          804 g         1412 t
ORIGIN      
        1 tcatttttat gaatgaaaag cataatatag gagaatcaac attgataaag ataaataatt
       61 tagatgaaat gaatcaattt gctatatttt tagttgagca attgaaaagt ggtgatttga
      121 ttttacttaa cggagattta ggagcaggta aaacaacgtt aacgcaattt ataggaaaag
      181 ctcttggtgt aagacgtacg attaattccc cgacatttaa catcattaaa tcatataggg
      241 gtaaaaattt aaaattgcat catatggatt gttatcgctt agaagattct gatgaagatt
      301 taggatttga tgaatttttc gaagatcagg caattactgt tattgaatgg agtcaattta
      361 taaaagattt acttccagcg acgcatttat ctattaacat ttcaacaata tctgaaaata
      421 caagacaaat tgagttgttc gcgcaaggag aacattatga acaaattaag gaggcaatta
      481 tccatgaatt cgctgctcat tgatacatcg aatcaaccat tatcagtagc attgatgcaa
      541 aacgatgaag ttttagctga aataactacg gattctaaac aaaatcattc agtgcaatta
      601 atgcctgcga ttagtcagct atttgagcag agcaaaatag ctaaacagca attagatgcg
      661 attattgttg ctgaaggtcc tggttcatat actggcttac gtatcggtgt tactgttgct
      721 aaaacattag cgtatgcgtt agatgttaaa ttatatggtg tttcatcatt aaaggcattg
      781 gcagcaacaa tcgatcatac tgataaatta ttggttcctg tgtttgatgc aagacgacaa
      841 gcagtttata caggcatatt ccagtggcaa aatggtcaat tagtgactat tttagaagat
      901 caatatatgt cgatagaggc attgcgagct tttttagaag atttaaacca accatttatt
      961 tatattggga aagatactgt aaaattacaa gatgaattac aaggtgaagt gattgcgcag
     1021 ctaccaaatg catcagtcat gtatcaattg ctagataaac cttcagacgt acatacattt
     1081 actcctaaat accataaatt agctgaggcg gaacgaaatt ggatcaacag tcaaaagaac
     1141 aattaaacat tagagagatg actaaagaag atgtgccaca agtctttgat cttgagcgtc
     1201 ggagtttcaa tgacagttct tggacaattg acgcatttta tcatgaaata gagcaaaata
     1261 actttgcgaa atattttgtc ttagaatttg aacaacaaat tattggttat ttaggtttat
     1321 ggattgttat cgatcaagct caaattacaa cagttgcaat cgatgataat tatagaggct
     1381 atggtttagg tcaaatgctt ttaaaatacg gaataaacta tgcaagtcac acttgtgatg
     1441 tgatgagttt agaagtaaga gttaataata aagtggcaca acatgtatat gaaaatttag
     1501 gatttcaata tggtggtaaa cgtaaaaatt attatggtga aggtgaggac gcaatggtta
     1561 tgtgggtgaa tttaaatgac taaagatata ttaatactag ctgttgaaac aagttgtgat
     1621 gaaacaagcg ttagtgttat aaaaaatggc agagatattt tatcaaatac agttttaagt
     1681 cagattgaaa gtcataaacg atttggcggt gtcgttcccg aagtggcaag tagacatcac
     1741 gttgaaggta taacagcaac aataaacgag gctctagggg atgccgatgt atcaatagaa
     1801 gatattgatg ccatagcggt tacagaaggc cctggactaa ttggtgcgtt actaataggt
     1861 gttaatgcag ccaaagcatt ggcatttgct tacgataagc cacttattcc tgttcatcat
     1921 attgcaggac atatatatgc taatcacata gaagagccat taacattccc gctaattgca
     1981 cttattgttt caggtggaca tactgaatta gtttatatga aggatcattt atcatttgaa
     2041 gtcattggtg aaacacgaga tgacgcagta ggtgaggctt atgataaagt ggcacgaaca
     2101 attggtttaa attatccagg tggtccacaa gttgatcggt tggctgctga aggtgaagat
     2161 acttattcat tccctcgtgt ttggttggat aaagatagtt atgattttag ttttagtggg
     2221 ttgaaaagtg ccgtaatcaa tcaacttcac aatcaacgac aaaaaaatat tccaatcatt
     2281 gaagctaacg tagcaacgag ctttcaaaac agtgttgtag aggtgctcac gtttaaagct
     2341 attcaagctt gtaaagaata tggtgttcag cgattaattg ttgctggtgg cgtggcgagt
     2401 aataaaggat tacgtcaatc tttagcggat caatgcaaag tcaatgacat tcaattaact
     2461 atcccaagtc ctaaattatg cacagataat gctgcaatga taggcgttgc cggccactat
     2521 ttgtatcagc aaggtcgatt tgctgattta gcattaaatg ggcacagcaa tatagattta
     2581 gaagagtatt ctgcagaata aaataaacgt cagttattaa catgattact atgaacatta
     2641 ttgaaactgt tcatagtctt ctgtcatgtg atagctgacg tttttatgtt taaagttaaa
     2701 tgctaaattt tcgggacatt atcttttgct cgttcatata tttttgctgg aaatgaagtg
     2761 atttttaata attcgatggc attacgtgta tttgctttgc caggcttaat tttgtaatca
     2821 aaatgtatgt tattattttc tattacctca ttgaaatggt aattttcata acgttgtttt
     2881 aagagttcag ctaactcaat atcatgtgtt gctgcaataa ctctaaagtt agatttttca
     2941 tgtaaaaatg atagtactga ttctgaagcg gcaattcgtt cagttgtgtt ggtaccttta
     3001 aaaatttcat ctataaagca gtatattttt tgattatctg gaatttcaac aatacgctta
     3061 atagatttta attcagccat aaaataacta tcgcctgata acacatcatc ggcatttgcc
     3121 attgacgtga acacaattcc aggttgatat acaaatttgc ttgcggtaac tgtttgtata
     3181 gccgatgcaa gaataatgtt tattgctatg gatttcataa atgttgattt acccgaagca
     3241 ttagacccag ttaacagtat attttgtgat agtgagaaat cattggctac tgcatctgct
     3301 atgagtggat gtgttaactc agagaatact atgccatcat ttgaatcatc aatttgtggt
     3361 tcagtataac attctagcgt tctacgatac attgctaatg cataatgatt atctaattca
     3421 gctatgtaat cgaaacaatt tttaagctca ttcatatgtg ttgtatagct tttttgaatt
     3481 gaatgaaata aaacataatc aagcatgaaa ataagtttaa ctagtttaat taccatagct
     3541 ccgatatctt catcattaac ttcagctaaa acactcgtta aatgacgtgc tgttctaaat
     3601 tgcttaaaat tgacttcagg ttgtggagca tgtttaattt ttgagatagt atagccttgt
     3661 tttaaaacat tggatgcata aaagatagat tttaagtcat cctcatatgt acgctttaaa
     3721 gttgctgata aaatgatatt aaataggcaa ctcataagaa ataataaaat accaacttgt
     3781 gaattaataa atataactgc aaatgagata actggtaaaa acgggcatag aaacaatata
     3841 ttttgacgtt ttaccggtgt gatttggtct ggaaatgttg gataaacagt tttaccaatc
     3901 aaagctaaat gatatgttac gttttttcta aattcagcat tatcattaaa taaactaagt
     3961 aatgatttgt tcgtaattga aagcattcca cgtaaacagg catatagctt catttcacca
     4021 atagctgtta aattataatt catcttatga aagattgcat tcatatttaa gtcggaccaa
     4081 gttttatcat caactaatga atgtgattca aatttttcct tgtagagctt atactcatag
     4141 tcgaaatgtg agttcggtct gataaaagct tctaatggag aaatcgtttg ccataatgtt
     4201 tccatagatt ttaatagacg ttgacgactc atgtatcgtc caacaattcc tattacagat
     4261 gtaagtagaa taattgctat aacaaatatt aaaaat
//
LOCUS       DAXWFK010000181         4227 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.182, whole genome shotgun sequence.
ACCESSION   DAXWFK010000181 DAXWFK010000000
VERSION     DAXWFK010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.182"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(104..4147)
                     /gene="sdrD"
                     /locus_tag="ACGEXS_002358"
     CDS             complement(104..4147)
                     /gene="sdrD"
                     /locus_tag="ACGEXS_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin SdrD"
                     /protein_id="HFF8013342.1"
                     /translation="MLNRENKTAITRKGMVSNRLNKFSIRKYTVGTASILVGTTLIFG
                     LGNQEAKAAESTNKELNEATTSASDNQSSDKVDMQQLNQEDNTKNDNQKEMVSSQGNE
                     TTSNGNKLIEKESVQSTTGNKVEVSTAKSDEQASPKSTNEDLNTKQTISNQEALQPDL
                     QENKSVVNVQPTNEENKKVDAKTESTTLNVKSDAIKSNDETLVDNNSNSNNENNADII
                     LPKSTAPKRLNTRMRIAAVQPSSTEAKNVNDLITSNTTLTVVDADKNNKIVPAQDYLS
                     LKSQITVDDKVKSGDYFTIKYSDTVQVYGLNPEDIKNIGDIKDPNNGETIATAKHDTA
                     NNLITYTFTDYVDRFNSVQMGINYSIYMDADTIPVSKNDVEFNVTIGNTTTKTTANIQ
                     YPDYVVNEKNSIGSAFTETVSHVGNKENPGYYKQTIYVNPSENSLTNAKLKVQAYHSS
                     YPNNIGQINKDVTDIKIYQVPKGYTLNKGYDVNTKELTDVTNQYLQKITYGDNNSAVI
                     DFGNADSAYVVMVNTKFQYTNSESPTLVQMATLSSTGNKSVSTGNALGFTNNQSGGAG
                     QEVYKIGNYVWEDTNKNGVQELGEKGVGNVTVTVFDNNTNTKVGEAVTKEDGSYLIPN
                     LPNGDYRVEFSNLPKGYEVTPSKQGNNEELDSNGLSSVITVNGKDNLSADLGIYKPKY
                     NLGDYVWEDTNKNGIQDQDEKGISGVTVTLKDENGNVLKTVTTDADGKYKFTDLDNGN
                     YKVEFTTPEGYTPTKVTSGSDIEKDSNGLTTTGVINGADNMTLDSGFYKTPKYNLGNY
                     VWEDTNKDGKQDSTEKGISGVTVTLKNENGEVLQTTKTDKDGKYQFTGLENGTYKVEF
                     ETPSGYTPTQVGSGTDEGIDSNGTSTTGVIKDKDNDTIDSGFYKPTYNLGDYVWEDTN
                     KNGVQDKDEKGISGVTVTLKDENDKVLKTVTTDENGKYQFTDLNNGTYKVEFETPSGY
                     TPTSVTSGNDTEKDSNGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQ
                     DSTEKGIKDVKVTLLNEKGEVIGTTKTDENGKYCFDNLDSGKYKVIFEKPAGLTQTGT
                     NTTEDDKDADGGEVDVTITDHDDFTLDNGYYEEETSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDRDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDAGKHTPVKPMSTTKDHHNKAKALPETGNENSGS
                     NNATLFGGLFAALGSLLLFGRRKKQNK"
BASE COUNT         1105 a          763 c          645 g         1714 t
ORIGIN      
        1 gttatttttt taaaaaaatt gcactattta atctcaaaca taagatacat ttcataaatt
       61 ttgaaataaa aaaccaggcc tcacggacct ggtataagct gtattattta ttttgttttt
      121 tacgacgacc gaataacaat aatgatccta atgctgcgaa taatccgcca aataacgttg
      181 cgttatttga gccgctattt tcattacctg tttctggtaa tgcttttgct ttattgtgat
      241 ggtctttagt agtactcatt ggtttaacag gtgtgtgctt acctgcatct gagtctgagt
      301 cgctgtctga gtctgaatcg ctgtctgagt ctgagtcgct atctgaatct gaatcgctat
      361 ctgagtctga gtcgctatct gagtctgaat cactatccga gtctgagtcg ctgtctgaat
      421 ctgagtcgct atccgaatct gagtcgctgt ctgaatctga gtcgctatct gagtctgaat
      481 cactatctga atctgagtcg ctatctgagt ccgaatcgct atctgaatct gagtcgctat
      541 ctgagtctga atcgctgtcc gaatctgagt cgctatctga atctgagtca ctatctgagt
      601 ctgagtcgct gtctgagtct gagtcgctgt ctgaatctga gtctctgtct gaatctgaat
      661 cgctatctga atctgaatcg ctgtctgaat ctgaatcgct atccgagtct gaatcactat
      721 ctgagtctga gtcgctgtct gaatctgagt cgctgtccga atctgagtcg ctatctgatg
      781 tttcttcttc gtagtagcca ttatcaagtg tgaaatcatc atgatccgta attgttacgt
      841 caacttcgcc accatctgca tctttatcat cttcagttgt atttgtacct gtttgtgtta
      901 agccagcagg cttttcaaaa ataactttgt atttaccgct atctaaatta tcaaagcagt
      961 atttaccatt ttcatctgtt ttagttgttc caattacttc gcctttttca tttaataaag
     1021 taactttaac atctttgata cctttttcag ttgaatcttg tttgccgtct ttattactgt
     1081 cgtaccaaac ataatcacct aaactatatt ttggtgtttt atagaaacca ctgtctaatg
     1141 tcatgttatc tgcatcttta atgacacctg ttgttgttaa accattagaa tctttttcag
     1201 tatcatttcc agaagttact gaagttggtg tataacctga tggtgtctcg aattcaactt
     1261 tataagttcc attgtttaaa tcagtgaatt gatatttacc attttcatct gttgtaactg
     1321 tttttaaaac tttgtcgttt tcatctttta acgtaactgt tacacctgaa atgccctttt
     1381 catctttatc ttgaacaccg tttttatttg tatcttccca tacatagtca cctaagttgt
     1441 aagtcggttt gtagaaacca gagtcaatag tatcgttatc tttatcttta atgacacctg
     1501 ttgttgatgt accatttgaa tctatacctt catcagttcc tgaacctact tgtgttggtg
     1561 tgtaacctga tggtgtttcg aattcaactt tataagttcc attttctaat ccagtaaatt
     1621 gatatttacc atctttatct gttttagttg tttgtaaaac ttcaccgttt tcatttttca
     1681 atgtaactgt tacgcctgaa ataccttttt cagttgaatc ctgcttacca tctttatttg
     1741 tatcttccca tacataatta cctaaattat attttggtgt tttgtagaat ccactatcta
     1801 atgtcatgtt atcagcacca ttaataacac ctgttgttgt taaaccatta gagtcttttt
     1861 caatgtcgct accagatgtt actttagtcg gtgtatagcc ttctggtgta gtaaattcaa
     1921 ctttataatt accattatct aaatcagtaa atttatattt gccatcagcg tctgttgtaa
     1981 ctgtttttaa cacgttaccg ttttcatctt ttaatgttac cgttacgcca gatatacctt
     2041 tttcatcttg gtcttggata ccatttttat ttgtatcttc ccagacatag tcacctaagt
     2101 tgtatttagg tttgtaaata cctaagtctg cagataagtt atctttgcca ttaactgtaa
     2161 taactgaaga taagccgttt gaatctaatt cttcgttatt accttgtttt gaaggggtta
     2221 cttcataacc ttttggtaag tttgaaaatt ctacacggta atctccatta ggtaagtttg
     2281 gaatcaagta tgacccatct tctttagtaa ctgcttctcc tacttttgta tttgtattat
     2341 tatcaaatac agttacagtt acattgccaa cgcctttttc tcctaattct tgaacaccgt
     2401 ttttattagt atcttcccat acgtagttac caattttata tacttcttga ccagctccgc
     2461 cactttggtt attagtaaat cctaaagcat tgccagtaga aacggattta ttacctgttg
     2521 aagataaagt agccatttga acaagtgttg ggctttcgct atttgtatat tggaattttg
     2581 tattaaccat tacaacataa gcagaatctg catttccaaa atcaataaca gcgctattgt
     2641 tgtcgccata tgtaattttc tgcaagtatt gatttgttac atctgtaagc tctttagtat
     2701 tcacatcgta tcctttattt aatgtataac ctttaggaac ttgatatatt tttatatctg
     2761 ttacatcttt atttatttgc ccgatattat taggataact tgagtggtaa gcttgaactt
     2821 ttagtttggc atttgttaaa gaattttccg atggatttac ataaatcgtt tgtttatagt
     2881 accctggatt ttctttattt ccaacatgtg aaactgtttc agtgaacgct gatccaattg
     2941 aatttttctc atttacaaca taatctggat attgaatgtt agcagttgtt tttgttgtag
     3001 tattacctat cgtaacatta aactcaacat cgtttttact aacaggaatt gtatcagcat
     3061 ccatataaat tgaataatta attcccattt gtacagaatt aaatcgatca acataatctg
     3121 taaatgtata tgtaattaaa ttatttgcag tatcatgttt tgcagtcgca attgtttcac
     3181 cattatttgg atctttaata tcaccaatat ttttaatatc ttccggattc aatccatata
     3241 cttgtactgt atctgagtat ttaattgtga aataatcacc tgatttaact ttgtcatcaa
     3301 ctgtaatttg tgattttaat gataaataat cttgggctgg tacgatttta ttgtttttat
     3361 ctgcatcaac gacagttaat gttgtatttg atgtgattaa atcattaaca tttttagcct
     3421 ctgttgatga tggctgtact gctgctatac gcattcttgt attcaaacgt ttaggtgctg
     3481 tactttttgg caaaatgata tctgcattat tttcattatt tgaattacta ttgttatcaa
     3541 caagagtttc atcattactc ttgatagcat cacttttaac atttaatgta gttgattcag
     3601 ttttggcatc tacctttttg ttttcctcat tagttggttg aacatttacc actgatttat
     3661 tctcttgcaa atcaggttgt aacgcttctt gattacttat agtttgttta gtgtttaaat
     3721 cttcattcgt agattttggt gaagcttgct catctgattt ggcagttgaa acttcaactt
     3781 tatttccagt ggtagattgt acactttctt tttctattaa tttattccca tttgaagtcg
     3841 tttcattacc ttgagatgat accatttctt tttgattatc atttttagta ttgtcttctt
     3901 gatttagttg ctgcatatca actttatcac tcgattgatt atcacttgct gaagttgtcg
     3961 cttcgttcaa ttctttatta gtactttctg cagcctttgc ttcttggttc cccagaccaa
     4021 aaattaatgt tgtacctact aaaattgatg ctgttcccac tgtgtacttt ctaatcgaaa
     4081 atttatttaa tcgattggat accatgcctt tccttgttat tgccgtttta ttttctctgt
     4141 ttagcattag attactccta attcatcaaa tttttaaata atacaattgt tttaaataca
     4201 aaaatgtata tcaatatagt atcacat
//
LOCUS       DAXWFK010000182         4206 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.183, whole genome shotgun sequence.
ACCESSION   DAXWFK010000182 DAXWFK010000000
VERSION     DAXWFK010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4206
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.183"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            218..3040
                     /gene="fnbB"
                     /locus_tag="ACGEXS_002359"
     CDS             218..3040
                     /gene="fnbB"
                     /locus_tag="ACGEXS_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein FnbB"
                     /protein_id="HFF8013343.1"
                     /translation="MKSNLRYGIRKHKLGAASVFLGTMIVVGMGQEKEAAASEQNNTT
                     VEESGSSATESKASETQTTTNNVNTIDETQSYSATSTEQPSQSTQVTTEEAPKTVQAP
                     KVETSRVDLPSEKVADKETTGTQVDIAQPSNVSEIKPRMKRSTDVTAVAEKEVVEETK
                     ATGTDVTNKVEVEEGSEIVGHKQDTNVVNPHNAERVTLKYKWKFGEGIKAGDYFDFTL
                     SDNVETHGISTLRKVPEIKSTDGQVMATGEIIGERKVRYTFKEYVQEKKDLTAELSLN
                     LFIDPTTVTQKGNQNVEVKLGETTVSKIFNIQYLGGVRDNWGVTANGRIDTLNKVDGK
                     FSHFAYMKPNNQSLSSVTVTGQVTKGNKPGVNNPTVKVYKHIGSDDLAESVYAKLDDV
                     SKFEDVTDNMSLDFDTNGGYSLNFNNLDQSKNYVIKYEGYYDSNASNLEFQTHLFGYY
                     NYYYTSNLTWKNGVAFYSNNAQGDGKDKLKEPIIEHSTPIELEFKSEPPVEKHELTGT
                     IEESNDSKPIDFEYHTAVEGAEGHAEGTIETEEDSIHVDFEESTHENSKHHADVVEYE
                     EDTNPGGGQVTTESNLVEFDEDSTKGIVTGAVSDHTTIEDTKEYTTESNLIELVDELP
                     EEHGQAQGPIEEITENNHHISHSGLGTENGHGNYGVIEEIEENSHVDIKSELGYEGGQ
                     NSGNQSFEEDTEEDKPKYEQGGNIVDIDFDSVPQIHGQNNGNQSFEEDTEKDKPKYEQ
                     GGNIIDIDFDSVPHIHGFNKHTEIIEEDTNKDKPNYQFGGHNSVDFEEDTLPQVSGHN
                     EGQQTIEEDTTPPIVPPTPPTPEVPSEPETPTPPTPEVPSEPETPTPPTPEVPTEPGK
                     PIPPAKEEPKKPSKPVEQGKVVTPVIEINEKVKAVVPTKKAQSKKSELPETGGEESTN
                     NGMLFGGLFSILGLALLRRNKKNHKA"
     gene            3220..4086
                     /gene="galU"
                     /locus_tag="ACGEXS_002360"
     CDS             3220..4086
                     /gene="galU"
                     /locus_tag="ACGEXS_002360"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFF8013344.1"
                     /translation="MKKIKKAIIPAAGLGTRFLPATKAMPKEMLPILDKPTIQYIVEE
                     AARAGIEDIIIVTGRHKRAIEDHFDSQKELEMVLKEKGKSELLEKVQYSTELANIFYV
                     RQKEQKGLGHAISSARQFIGNEPFAVLLGDDIVESEVPAVKQLIDVYEETGHSVIGVQ
                     EVPEADTHRYGIIDPLTKNGRQYEVKKFVEKPAQGTAPSNLAIMGRYVLTPEIFDYLK
                     TQKEGAGNEIQLTDAIERMNNDNQVYAYDFEGERYDVGEKLGFVKTTIEYALKDDSMR
                     EELTRFIKALGL"
BASE COUNT         1660 a          638 c          837 g         1071 t
ORIGIN      
        1 aaatacaagc ctgttatatc aaggtgttca agagctttgt atgcaatata tatgtgagtt
       61 tcaaataata aaaaactcat tatacataaa gttaacaaca atcttaactt tttattaact
      121 cgcttttttt cattgctttt aaaaaccgaa caatatagaa ttgcatttat tgagttttaa
      181 aaataaatga attttgcatt taagggagaa tattatagtg aaaagcaatc ttagatacgg
      241 cataagaaaa cacaaattgg gagcggcctc agtattctta ggaacaatga tcgttgttgg
      301 aatgggacaa gaaaaagaag ctgcagcatc ggaacaaaac aatactacag tagaggaaag
      361 tgggagttca gctactgaaa gtaaagcaag cgaaacacaa acaactacaa ataacgttaa
      421 tacaatagat gaaacacaat catacagcgc gacatcaact gagcaaccat cacaatcaac
      481 acaagtaaca acagaagaag caccgaaaac tgtgcaagca ccaaaagtag aaacttcgcg
      541 agttgatttg ccatcggaaa aagttgctga taaggaaact acaggaactc aagttgacat
      601 agctcaacca agtaacgtct cagaaattaa accaagaatg aaaagatcaa ctgacgttac
      661 agcagttgca gagaaagaag tagtggaaga aactaaagcg acaggtacag atgtaacaaa
      721 taaagtggaa gtagaagaag gtagtgaaat tgtaggacat aaacaagata cgaatgttgt
      781 aaatcctcat aacgcagaaa gagtaacctt gaaatataaa tggaaatttg gagaaggaat
      841 taaggcggga gattattttg atttcacatt aagcgataat gttgaaactc atggtatctc
      901 aacactgcgt aaagttccgg agataaaaag tacagatggt caagttatgg cgacaggaga
      961 aataattgga gaaagaaaag ttagatatac gtttaaagaa tatgtacaag aaaagaaaga
     1021 tttaactgct gaattatctt taaatctatt tattgatcct acaacagtga cgcaaaaagg
     1081 taaccaaaat gttgaagtta aattgggtga gactacggtt agcaaaatat ttaatattca
     1141 atatttaggt ggagttagag ataattgggg agtaacagct aatggtcgaa ttgatacttt
     1201 aaataaagta gatgggaaat ttagtcattt tgcgtacatg aaacctaaca accagtcgtt
     1261 aagctctgtg acagtaactg gtcaagtaac taaaggaaat aaaccagggg ttaataatcc
     1321 aacagttaag gtatataaac acattggttc agacgattta gctgaaagcg tatatgcaaa
     1381 gcttgatgat gtcagcaaat ttgaagatgt gactgataat atgagtttag attttgatac
     1441 taatggtggt tattctttaa actttaataa tttagaccaa agtaaaaatt atgtaataaa
     1501 atatgaaggg tattatgatt caaatgctag caacttagaa tttcaaacac acctttttgg
     1561 atattataac tattattata caagtaattt aacttggaaa aatggcgttg cattttactc
     1621 taataacgct caaggcgacg gcaaagataa actaaaggaa cctattatag aacatagtac
     1681 tcctatcgaa cttgaattta aatcagagcc gccagtggag aagcatgaat tgactggtac
     1741 aatcgaagaa agtaatgatt ctaagccaat tgattttgaa tatcatacag ctgttgaagg
     1801 tgcagaaggt catgcagaag gtaccattga aactgaagaa gattctattc atgtagactt
     1861 tgaagaatcg acacatgaaa attcaaaaca tcatgctgat gttgttgaat atgaagaaga
     1921 tacaaaccca ggtggtggtc aggttactac tgagtctaac ctagttgaat ttgacgaaga
     1981 ttctacaaaa ggtattgtaa ctggtgctgt tagcgatcat acaacaattg aagatacgaa
     2041 agaatatacg actgaaagta atctgattga actagtagat gaactacctg aagaacatgg
     2101 tcaagcgcaa ggaccaatcg aggaaattac tgaaaacaat catcatattt ctcattctgg
     2161 tttaggaact gaaaatggtc acggtaatta tggcgtgatt gaagaaatcg aagaaaatag
     2221 ccacgtggat attaagagtg aattaggtta cgaaggtggc caaaatagcg gtaatcagtc
     2281 atttgaggaa gacacagaag aagataaacc gaaatatgaa caaggtggca atatcgtaga
     2341 tatcgatttc gatagtgtac ctcaaattca tggtcaaaat aatggtaacc aatcattcga
     2401 agaagataca gagaaagaca aacctaagta tgaacaaggt ggtaatatca ttgatatcga
     2461 cttcgacagt gtgccacata ttcacggatt caataagcac actgaaatta ttgaagaaga
     2521 tacaaataaa gataaaccaa attatcaatt cggtggacac aatagtgttg actttgaaga
     2581 agatacactt ccacaagtaa gtggtcataa tgaaggtcaa caaacgattg aagaagatac
     2641 aacacctcca atcgtgccac caacgccacc gacaccagaa gtaccaagcg agccggaaac
     2701 accaacacca ccgacaccag aagtaccaag cgagccggaa acaccaacac cgccaacgcc
     2761 agaggtacca actgaacctg gtaaaccaat accacctgct aaagaagaac ctaaaaaacc
     2821 ttctaaacca gtggaacaag gtaaagtagt aacacctgtt attgaaatca atgaaaaggt
     2881 taaagcagtg gtaccaacta aaaaagcaca atctaagaaa tctgaactac ctgaaacagg
     2941 tggagaagaa tcaacaaaca acggcatgtt gttcggcgga ttatttagca ttttaggttt
     3001 agcgttatta cgcagaaata aaaagaatca caaagcataa tcaatccaaa attgacaggt
     3061 ttatttcata aattatatga agtaagcctg ttttttaaaa ttaaaacaaa tttcccaaga
     3121 aataattaca tactcaatga cactatgaag gcgttctaat tagtgttaaa atgacgttga
     3181 tacatagatt taatacttag gaaaaggagc acattaactt tgaaaaaaat aaaaaaggca
     3241 atcattcccg ctgctggttt agggactaga tttttaccag caactaaagc gatgccaaag
     3301 gaaatgcttc ctatcttaga taaacccaca atacaatata tcgttgaaga agctgcaaga
     3361 gctggaattg aagatattat tatagtgaca ggtcgccaca aacgcgcgat tgaagatcat
     3421 tttgatagtc aaaaagaatt agaaatggtg ttaaaagaaa aaggtaaatc tgaattacta
     3481 gagaaagttc agtattcaac ggaacttgcg aatatttttt atgtaaggca gaaagaacaa
     3541 aaaggtttag ggcatgcgat tagttctgcg cgtcaattta tcggcaatga accatttgcg
     3601 gtattattag gtgacgatat tgttgaatct gaagtgccag cagtgaaaca gttgattgac
     3661 gtttatgaag aaacagggca ctcagttata ggtgtccaag aagttccgga agcagatact
     3721 catcgttatg gaattattga cccattaact aaaaacggac gtcagtatga agtgaaaaag
     3781 tttgttgaaa agccagcaca aggtacggca ccatcaaacc tagctattat gggacgttat
     3841 gtattaacgc ctgaaatttt cgattattta aaaacgcaaa aagaaggcgc aggtaacgaa
     3901 attcagttaa cagatgcgat tgaacgtatg aataatgata accaagtgta tgcatatgac
     3961 ttcgaaggcg aacgttatga cgttggagaa aaattaggct tcgttaaaac taccatagaa
     4021 tatgcattga aagatgacag tatgcgagaa gaattaacac gatttattaa agcattaggt
     4081 ttataacatt aaaagcatga tactcagtaa aattggaaca cgaaatggtg aactaaattg
     4141 aatgagtgtc atgcttaatt tatgtgttgt gatatatagt agggtattga aataatataa
     4201 aaacaa
//
LOCUS       DAXWFK010000183         4125 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.184, whole genome shotgun sequence.
ACCESSION   DAXWFK010000183 DAXWFK010000000
VERSION     DAXWFK010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.184"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            122..883
                     /gene="sufC"
                     /locus_tag="ACGEXS_002361"
     CDS             122..883
                     /gene="sufC"
                     /locus_tag="ACGEXS_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFF8013345.1"
                     /translation="MASTLEIKDLHVSIEDKEILKGVNLTINTDEIHAIMGPNGTGKS
                     TLSSAIMGHPSYEVTKGEVLLDGVNILELEVDERAKAGLFLAMQYPSEITGVTNADFM
                     RSAINAKREEGQEINLMQFIKKLDKNMDFLDIDKDMAQRYLNEGFSGGEKKRNEILQL
                     MMLEPKFAILDEIDSGLDIDALKVVSKGINQMRGENFGALMITHYQRLLNYITPDKVH
                     VMYAGQVVKSGGPELAKRLEEEGYEWVKEEFGSAE"
     gene            981..2288
                     /gene="sufD"
                     /locus_tag="ACGEXS_002362"
     CDS             981..2288
                     /gene="sufD"
                     /locus_tag="ACGEXS_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFF8013346.1"
                     /translation="MTTDILNISEEQLVDYSKAHNEPSWMTELRKKALKLTETLEMPK
                     PDKTKLRKWDFDSFKQHDVKGDVYQSLSQLPESVREIIDVDHSKNLVIQHNNTIAYTQ
                     VDDNASKDGVIVEGLADALMNHSDLVQKYFMKDAVTVDEHRITALHTALVNGGVFVYV
                     PKNVVVEHPVQYVVLHDDENASFYNHVIIVTEESAEVTYVENYLSNASGEGNQLNIIS
                     EVIAGANSNITYGSVDYMDKGFTGHIIRRGITEADASINWALGLMNEGSQIIDNTTNL
                     FGDRSTSSLKSVVVGTGEQKINLTSKIVQYGKETDGYILKHGVMKEHASSVFNGIGYI
                     KHGGTKSIANQESRVLMLSEHARGDANPILLIDEDDVQAGHAASVGRVDPDQLYYLMS
                     RGISQREAERLVIHGFLDPVVRELPIEDVKRQLREVIERKVSK"
     gene            2394..3644
                     /locus_tag="ACGEXS_002363"
     CDS             2394..3644
                     /locus_tag="ACGEXS_002363"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFF8013347.1"
                     /translation="MNEVAEHSFDVNEVIKDFPILDQKVNGKRLAYLDSTATSQTPVQ
                     VLNVLEDYYKRYNSNVHRGVHTLGSLATDGYENARETVRRFINAKYFEEIIFTRGTTA
                     SINLVAHSYGDANVEEGDEIVVTEMEHHANIVPWQQLAKRKNATLKFIPMTADGELNI
                     EDIKQTINDKTKIVAIAHISNVLGTINDVKTIAEIAHQHGAIISVDGAQAAPHMKLDM
                     QEMNADFYSFSGHKMLGPTGIGVLFGKRELLQKMEPIEFGGDMIDFVSKYDATWADLP
                     TKFEAGTPLIAQAIGLAEAIRYLERIGFDAIHKYEQELTIYAYEQMSAIEGIEIYGPP
                     KDRRAGVITFNLQDVHPHDVATAVDTEGVAVRAGHHCAQPLMKWLNVSSTARASFYIY
                     NTKEDVDQLINALKQTKEFFSYEF"
     gene            3634..4098
                     /gene="sufU"
                     /locus_tag="ACGEXS_002364"
     CDS             3634..4098
                     /gene="sufU"
                     /locus_tag="ACGEXS_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFF8013348.1"
                     /translation="MNFNNLDQLYRSVIMDHYKNPRNKGVLDNGSMTVDMNNPTCGDR
                     IRLTFDIEDGIIKDAKFEGEGCSISMASASMMTQAVKGHSLGEAMQMSQEFTKMMLGE
                     DYVITEEMGDIEALQGVSQFPARIKCATLAWKALEKGTVAKEGKAEGTTEEE"
BASE COUNT         1483 a          587 c          834 g         1221 t
ORIGIN      
        1 atattaaggt ataagtaagt tataattaac tgaacgcatt attacaaagt ctttttgact
       61 acaaattaaa attattataa actagttaag aaaactttat attttacgga gggaatataa
      121 aatggcatca acattagaaa tcaaagacct acatgtgtct attgaggata aagaaatctt
      181 aaaaggtgtt aacttgacaa ttaacactga tgaaatacat gcgattatgg gaccaaacgg
      241 gacaggtaaa tcaactttat catctgcaat tatgggacac ccaagctatg aagtaactaa
      301 aggagaagta cttttagacg gtgtaaatat tttagaatta gaagttgatg aaagagcaaa
      361 agcaggatta ttcttggcaa tgcaatatcc atcagaaatt acaggtgtta caaatgctga
      421 tttcatgcgt tcagcaatca atgcgaaacg tgaagaagga caagaaatca acttaatgca
      481 atttattaag aaattagata aaaacatgga ttttctagac atagataaag acatggcaca
      541 acgttattta aatgaaggtt tctcaggtgg agagaagaaa cgtaacgaaa tcttacaatt
      601 aatgatgtta gaacctaagt ttgcaatctt agatgaaatc gattcagggt tagacatcga
      661 tgcattaaaa gttgtatcta aaggtattaa ccaaatgcgt ggggaaaact ttggtgcatt
      721 aatgattaca cactatcaac gattattaaa ttacattact cctgataaag tacatgtaat
      781 gtatgctggt caagtcgtta aatctggtgg tccagaatta gcaaaacgtc ttgaagaaga
      841 aggatatgaa tgggttaaag aagagttcgg ttcagctgaa taatcttatt aatacagtat
      901 ccatgagatg ttcatctata tatgatgaaa atgaacattt atacgaaata gtaaatttca
      961 tcaagtagga ggaaaaagtt atgacaactg atattttgaa catttctgaa gaacaacttg
     1021 ttgattattc taaagcccac aatgaacctt cttggatgac agaattacgt aaaaaagctt
     1081 tgaaattaac agaaacttta gaaatgccaa aacctgataa aacaaaatta agaaaatggg
     1141 attttgattc ttttaaacaa cacgatgtaa aaggtgatgt ttatcaatct ttatcacaat
     1201 tacctgagtc agtaagagaa attattgacg tagatcattc taaaaactta gtaattcaac
     1261 ataataatac gattgcgtac acacaagttg atgataatgc atcgaaagat ggcgttatcg
     1321 ttgaaggttt agcagacgct cttatgaacc atagtgattt agtacaaaag tactttatga
     1381 aagatgcagt aacagtagat gaacatcgta tcacagcgct acacacggca ttagttaatg
     1441 gtggcgtatt tgtttatgtt cctaaaaatg tagttgtaga acatccagta caatacgttg
     1501 tgttgcacga cgacgaaaat gcaagctttt ataaccatgt tatcatcgtt actgaagaaa
     1561 gcgccgaagt cacatatgtt gaaaattact tatcaaatgc atctggtgaa ggaaatcaat
     1621 taaatattat ttctgaagtg attgctggtg caaattcaaa tatcacatat ggctcagtgg
     1681 actatatgga taaaggcttt acaggtcata tcattcgacg tggtattact gaagcggatg
     1741 cctcaattaa ttgggcacta ggtttaatga atgagggtag ccaaattatt gataatacaa
     1801 caaatttatt tggtgatcgt tcaacaagtt cacttaaatc agtagttgta ggtacaggcg
     1861 aacaaaaaat taatctaaca tctaaaatcg tacaatatgg taaagaaaca gatggttata
     1921 tccttaaaca tggtgttatg aaagaacatg catcgtctgt atttaatggt atcggctaca
     1981 ttaagcatgg tggaactaaa tcaattgcta atcaggaatc acgtgtatta atgttatctg
     2041 aacatgctcg tggtgacgcg aatcctattt tattaattga tgaagatgat gtacaagctg
     2101 gtcatgctgc atcagtaggt cgtgttgatc cagatcaact ttactattta atgagtcgtg
     2161 gtatttctca aagagaagcg gaacgtcttg ttatacatgg tttcttagat ccagtagtac
     2221 gtgaattacc tatcgaagac gttaaacgtc aattgagaga agtaattgaa cgcaaagttt
     2281 ctaaataata ttttgaaaat aaaagtttgt aatagatata gactgtcgat attggtataa
     2341 gactaataca acgtcagtat ttaaatgatt aggattttta tttaagaaag gtcgtgaatg
     2401 aagtggccga acactcattt gacgttaatg aagtaatcaa ggattttccg atattagatc
     2461 aaaaagtcaa tggcaaacgt ttagcatatc ttgattcaac agcgacaagt caaacgcctg
     2521 tgcaagtgtt aaatgtttta gaagattact acaagcgtta taattcaaac gttcatcgtg
     2581 gtgttcatac attaggatca ttggcaactg atggttatga aaatgcccgt gaaaccgttc
     2641 gtcgttttat taatgcgaag tattttgaag aaatcatttt tacacgcgga acaactgcgt
     2701 cgattaacct tgtagcacat agctatggtg atgcaaatgt tgaagagggc gatgaaattg
     2761 ttgtcactga aatggaacat catgccaata ttgttccttg gcaacagtta gcaaagcgta
     2821 aaaatgcgac attgaaattt ataccaatga cagctgacgg tgaattaaac atcgaggata
     2881 ttaagcaaac gattaatgat aaaacaaaga tcgttgctat tgcacatata tctaatgtgc
     2941 tcggtacaat taatgatgtt aaaaccattg cagaaatagc tcatcaacat ggtgcaatta
     3001 tcagtgttga tggggcgcaa gcagcaccac atatgaaact tgatatgcaa gaaatgaatg
     3061 ctgattttta tagttttagt ggtcataaaa tgcttggacc aacaggtatt ggcgtattat
     3121 ttggtaaacg tgagttacta caaaaaatgg aaccgattga gttcggtggc gacatgattg
     3181 attttgtaag taagtatgat gcaacatggg ctgatttacc tactaaattt gaggcgggta
     3241 ctccattaat tgctcaagca attgggcttg cagaagctat tcgctattta gaacgcatag
     3301 gttttgatgc aattcataaa tatgaacaag aattaacgat atatgcttat gagcaaatgt
     3361 ctgcaattga aggaattgaa atttatggcc cgccaaagga tcgtcgtgca ggtgtaataa
     3421 cgtttaattt acaagatgta catccacacg atgttgctac agccgtagat acagaaggtg
     3481 tagcggttag agctgggcat cattgtgcgc aaccgttaat gaaatggtta aatgtgtctt
     3541 caacagctag agcgagtttt tatatataca acacgaaaga agacgttgat cagttaataa
     3601 atgccttgaa acaaacgaag gagtttttct cttatgaatt ttaataatct agatcaatta
     3661 tatagatctg tcattatgga tcattataaa aatcctagaa ataaaggtgt attagataac
     3721 gggtctatga cagtagatat gaataacccg acatgcggtg accgtatacg actaacattt
     3781 gatatagaag acggcattat aaaagatgct aagtttgaag gtgaaggttg ttcgatttca
     3841 atggcaagtg catcgatgat gacacaagct gttaaaggtc attcacttgg agaagcaatg
     3901 caaatgagcc aagaatttac gaaaatgatg cttggtgaag actatgtgat tacagaagaa
     3961 atgggagata ttgaagcatt gcaaggtgta tctcaattcc cagctcgtat taaatgtgcc
     4021 acattagctt ggaaagcatt ggaaaaaggt actgttgcta aagaaggtaa agcagaaggt
     4081 acgactgaag aagaatagaa tgctgttaat catagataat tttga
//
LOCUS       DAXWFK010000184         4034 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.185, whole genome shotgun sequence.
ACCESSION   DAXWFK010000184 DAXWFK010000000
VERSION     DAXWFK010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.185"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            39..1670
                     /locus_tag="ACGEXS_002365"
     CDS             39..1670
                     /locus_tag="ACGEXS_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFF8013349.1"
                     /translation="MKKLTTILFQYKIFPVLMFLVSTGLGIIVITQNILIADFLAKII
                     RHQFQGLWIVLFILLGVLLLRATVQFLNQWLGDTLAFKVKHMLRQRVIYKNNGHPIGE
                     QMTILTENIDGLAPFYKSYLPQVFKSMMVPLIIIIAMFFIHFNTALIMLITAPFIPLF
                     YIIFGLKTRDESKDQMTYLNQFSQRFLNIAKGLVTLKLFNRTEQTEKHIYDDSTQFRT
                     LTMRILRSAFLSGLMLEFISMLGIGLVALEATLSLVVFHNIDFKTAAIAIILAPEFYN
                     AIKDLGQAFHTGKQSEGASDVVFEFLEQPNYNNEFLLKYEENQKPFIQLTDISFRYDD
                     SDRLVLNDLNLEIFKGDQIALVGPSGAGKSTLTHLIAGVYQPTIGTISTNQRDLNIGI
                     LSQQPYIFSASIKENITMFKDIENNTIEEVLDEVGLLDKVQSFTKGINTIIGEGGEML
                     SGGQMRRIELCRLLVMKPDLVIFDEPATGLDIQTEHMIQNVLFQHFKDTTMIVIAHRD
                     NTIRHLQRRLYIENGRLIADDRNISVNITENGDDL"
     gene            1667..3340
                     /locus_tag="ACGEXS_002366"
     CDS             1667..3340
                     /locus_tag="ACGEXS_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding/permease
                     protein"
                     /protein_id="HFF8013350.1"
                     /translation="MKTRLKFQVDKDLLLAIVVGVCGSLVALAMFFLSGYMVTQSALG
                     APLYALMILVVTVKLFGFLRAITRYVERLISHKATFTMLRDIRVQFFGKLVNVIPNVY
                     RKLSSSDLISRMISRVEALQNIYLRVYYPPVVIGLTALVTVIVLAFISIGHALLIMVS
                     MLFTLLIVPWLSSKKARTLKKHAANEQARFLNHFYDYKAGMDELRRFNQINHYRDNLM
                     AKLNHFDKLQLKEQRFLTIYDFILNIIAMLSIFGSLVLGLIQINAGQLNIIYMTSIVL
                     MVLTLFEQAVPMTNVAYYKADTDQALHDINEVISVPSTNGKKRLNDKYDATNIYEVKD
                     ASFKYWNQQTYVLSDINFNVNRGEKIAIVGPSGSGKSTLLQIMAGLYQLDSGSVRFEN
                     MDMFEIDDKDKFESLNVLLQSQQLFDGTIRQNLFTDEKDEAVQAIFKQLDLEHLALER
                     QIDLDGHTLSGGEIQRLAITRMLLKDTASTWILDEPTTALDKQNSLKVMDLIEAHAET
                     LIVATHDLTLLSRFETIIVMINGKIVEKGNYQQLLANQGALWNMIQYNA"
     gene            complement(3467..3910)
                     /gene="mgrA"
                     /locus_tag="ACGEXS_002367"
     CDS             complement(3467..3910)
                     /gene="mgrA"
                     /locus_tag="ACGEXS_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MgrA"
                     /protein_id="HFF8013351.1"
                     /translation="MSDQHNLKEQLCFSLYNAQRQVNRYYSNKVFKKYNLTYPQFLVL
                     TILWDESPVNVKKVVTELALDTGTVSPLLKRMEQVDLIKRERSEVDQREVFIHLTDKS
                     ETIRPELSNASDKVASASSLSQDEVKELNRLLGKVIHAFDETKEK"
BASE COUNT         1337 a          575 c          722 g         1400 t
ORIGIN      
        1 aaggaaaacc ttttataata ggtttaggtg atataattgt gaaaaaatta acaacaatac
       61 tgtttcaata taaaattttt ccggtactca tgttcttggt cagtactggt ctcggcataa
      121 tcgttataac gcaaaatatt ttaatagcag attttttagc taaaattata agacatcaat
      181 ttcaaggttt atggattgta ttatttattt tattaggtgt tttactttta agagcaactg
      241 tgcaatttct aaatcaatgg ttaggtgata cattagcatt taaagttaag catatgctta
      301 gacagcgggt tatttataaa aataatggtc atccaatcgg tgaacaaatg actatactca
      361 cagaaaacat tgatggtcta gcaccttttt ataagagtta tttgcctcaa gtgttcaaat
      421 caatgatggt tccgctcatc ataatcattg caatgttttt catccatttc aataccgcat
      481 taattatgtt aataactgca ccatttattc ctttgtttta tattattttc ggtttgaaaa
      541 cgcgagatga gtcaaaagat caaatgactt atttgaatca atttagtcaa cggtttttaa
      601 atattgctaa aggtttagtg acgttaaagc tatttaatcg tacagagcaa acagagaagc
      661 atatttacga cgatagtact cagtttagaa ctttaacaat gcgcatttta cgcagtgctt
      721 ttttatcggg attaatgctc gaatttataa gtatgttagg tattggattg gttgcattgg
      781 aagcaacgct aagcttagta gtatttcata atattgattt taaaactgcg gcaattgcga
      841 ttattttagc gcctgaattt tataatgcaa ttaaggactt agggcaagcg ttccatactg
      901 gaaaacaaag tgaaggtgcc agtgacgttg tgtttgagtt tttagaacaa ccgaactata
      961 ataatgaatt tctattaaag tatgaggaaa accaaaagcc atttattcag ttaacagaca
     1021 tatcatttcg atatgatgat tctgatagat tggtattaaa tgatttaaat ttggaaatat
     1081 ttaaaggtga tcaaattgca cttgtaggtc caagcggggc aggtaaatcc actttgacac
     1141 atcttattgc aggtgtttat cagccaacaa taggtactat aagtacaaac cagcgtgatt
     1201 taaatatagg aatacttagt caacagccat atattttcag tgcttctata aaagagaata
     1261 ttacgatgtt taaagatata gaaaataata ctattgaaga agtgctagac gaagtaggtt
     1321 tattagacaa agtgcaatct ttcacaaaag gcattaacac aataataggt gaaggaggcg
     1381 aaatgttatc tggtggacag atgagacgca tagaactttg ccgtctttta gttatgaagc
     1441 cagatctcgt tatatttgat gagcctgcaa ctggtttaga tattcaaaca gaacacatga
     1501 ttcagaacgt tctgtttcaa cattttaaag atacaacgat gattgtcatt gcacatagag
     1561 ataatacaat tcgccattta caacgacgct tgtatataga aaatggaaga ctgattgctg
     1621 atgatcgcaa tatttcagta aatataacag aaaatggtga tgacttatga aaacacgact
     1681 aaaatttcaa gtagataagg atttattgtt agctatagtt gttggtgttt gtggaagttt
     1741 agttgcgctc gccatgtttt tcttaagtgg ttatatggtg acacaaagtg cacttggtgc
     1801 gccactatac gctctgatga ttttagtcgt tacagtaaaa ttgtttgggt ttttaagagc
     1861 tattactcga tacgtagagc gccttatttc tcataaagct acatttacaa tgctacgtga
     1921 tattcgggta cagtttttcg gtaaattagt aaatgtcatt cctaatgttt accgtaaact
     1981 gagttctagt gatttaattt cacgtatgat tagtcgtgtt gaggcattac aaaatatata
     2041 tttacgtgtt tattatccac cagtcgtcat cggtttgaca gcgctagtta cagtcatagt
     2101 tttggcgttc atttcaatcg gccatgcgct attgattatg gttagcatgt tgttcacttt
     2161 actcattgtt ccttggttaa gctcaaaaaa agcacgtact ttaaagaaac atgcagctaa
     2221 tgaacaggcc cgatttttaa atcattttta tgattataaa gctggtatgg atgaactacg
     2281 tcgatttaat caaattaatc attatcgaga taatttgatg gctaaattaa atcattttga
     2341 taaattacaa cttaaagagc aacgcttttt aacgatttat gattttatat taaatattat
     2401 tgctatgctt tcgatttttg gtagtttagt tctaggatta attcaaatta atgcaggcca
     2461 actaaatatt atttatatga cgagtatagt tttaatggtc ttaactttat ttgaacaagc
     2521 tgtaccaatg acaaatgtcg cgtattataa agcggatact gaccaagcat tgcacgatat
     2581 taatgaagtg atatctgtac cttctactaa tggaaaaaaa cgtcttaatg ataagtatga
     2641 tgcaacgaac atttatgaag ttaaggatgc tagttttaag tattggaatc agcaaacgta
     2701 tgtgttgtcg gatattaatt ttaatgttaa tagaggcgaa aagattgcga ttgtgggtcc
     2761 ttctggttca ggaaaaagta cattactaca aattatggca gggttatatc aattagatag
     2821 tggctctgtt cgtttcgaaa atatggatat gtttgaaata gatgacaaag ataagtttga
     2881 atcgttaaat gtcttgctac aatctcaaca attatttgat ggtacaatac gtcaaaattt
     2941 atttaccgat gaaaaagatg aagcggtgca agcaatattt aagcaattag atttagaaca
     3001 tttggcacta gaacgtcaaa ttgacttaga tggtcataca ttatctggcg gagaaattca
     3061 gcgtttagcg attacgagga tgttattaaa agatactgca tcaacatgga ttttagatga
     3121 accaacaact gcattagata aacaaaatag tttaaaagtt atggatttaa ttgaagcaca
     3181 tgcagaaaca ttaattgttg ctacacacga tttaacttta ttgtcacgtt ttgagaccat
     3241 cattgtgatg ataaatggta aaatagttga aaagggaaac tatcaacaat tactagctaa
     3301 tcaaggtgct ttatggaata tgattcaata taatgcataa aaaaactgct tgataggcta
     3361 agaaaccttt caagcagttt tttaactaac aagcttacgc gtgctaattg tttttctatt
     3421 cttaataaat tctaaacatt actttaattg tcatgacaaa agttaattat ttttcctttg
     3481 tttcatcaaa tgcatgaatg actttaccta ataagcgatt aagttcttta acttcatctt
     3541 gcgataaaga agaagctgaa gcgactttgt cagatgcatt acttaattct ggtctaatag
     3601 tttcactttt gtcagtcaag tgaataaata cttcacgttg atcgacttcg gaacgttcac
     3661 gcttaattaa gtctacttgt tccattcgtt ttaataatgg tgatactgta ccagtatcga
     3721 gtgctaattc agttacgact ttcttgacgt ttacaggaga ttcatcccat aaaattgtta
     3781 agacaagaaa ttgtgggtat gttagattgt acttcttaaa aactttgtta gagtagtagc
     3841 gattaacttg tctttgagca ttgtacaaac taaagcatag ctgttctttt aaattatgtt
     3901 gatcagacat taaagttctc ctccagacat actatccgtt tttttctctt ttcggattgg
     3961 taatcattaa aaagttgatt gtttattaat tcacaacttt ctttgattca atgccatgct
     4021 aaaattaaag tatg
//
LOCUS       DAXWFK010000185         3998 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.186, whole genome shotgun sequence.
ACCESSION   DAXWFK010000185 DAXWFK010000000
VERSION     DAXWFK010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3998
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.186"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            22..198
                     /locus_tag="ACGEXS_002368"
     CDS             22..198
                     /locus_tag="ACGEXS_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013352.1"
                     /translation="MKHLTKIFVVLAIILFIIGYYLQATNHESQGIKLLLAAIMFMIC
                     AFISRSNDRRKNSK"
     gene            457..837
                     /locus_tag="ACGEXS_002369"
     CDS             457..837
                     /locus_tag="ACGEXS_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8013353.1"
                     /translation="MKIILKNNSDFPIYEQIKQQVKQNILKGHVAPGEHLPSMRELAK
                     DLQVSLITTKRAYEDLEKDGFVTTIRGKGTFVKEQDSSILKEKQFFTIENLVKELVNE
                     AQAIEMSLEELQDILTFIYEEESS"
     gene            834..1730
                     /gene="pmtA"
                     /locus_tag="ACGEXS_002370"
     CDS             834..1730
                     /gene="pmtA"
                     /locus_tag="ACGEXS_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     ATP-binding protein PmtA"
                     /protein_id="HFF8013354.1"
                     /translation="MNAIELSNVNYSSDQFNLKNISFKVPQGFVTGFIGRNGAGKTTI
                     IRLIMDLYQPQTGVIRVLEEDMALNPIELKNRIGFVYSENYFNERWTTKQLEKMIAPF
                     YRKWDHQVFEFYLEKFDLPINKSIKTFSTGMKMKLSLAVAFSHHAELYIFDEPTSGLD
                     PLARNELLEIIQQELIDENKTIFMSTHIISDLEKIADYIIHLSDGEVILNGSKEQLLQ
                     RYQVVSGAIEDLDDELASLLIYEEHKRTGFIGLTEHAQVFKEILGHKVNITTPSIENL
                     MVYLEKRKPKYHENIKLMEEGF"
     gene            1731..2411
                     /gene="pmtB"
                     /locus_tag="ACGEXS_002371"
     CDS             1731..2411
                     /gene="pmtB"
                     /locus_tag="ACGEXS_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtB"
                     /protein_id="HFF8013355.1"
                     /translation="MKALLIRNFKLRRYTLIIYVLLLTLYPFYIMLDSTKFFYLLQSF
                     ISPTILIIWILDAGHLFRLNRRLGGNDSYYFYMSLPVSKKQLLNANYITCIVLTLIGT
                     LVISLYAYEADVIEPNSIYFSTAYAFVISNFLSIPIAFSQFTELRRVKVPYGIYVFTI
                     IILVPFLFSIAIVLVNYFVLSQSSFPDLYSYILNIGFLIISIVILIVNYFKQLNKINT
                     RKFKGGSR"
     gene            2408..3280
                     /gene="pmtC"
                     /locus_tag="ACGEXS_002372"
     CDS             2408..3280
                     /gene="pmtC"
                     /locus_tag="ACGEXS_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     ATP-binding protein PmtC"
                     /protein_id="HFF8013356.1"
                     /translation="MKLEHITKKYGSNVVLNDIDFDFGDSRIVGLIGKNGVGKTTVMK
                     VMNGNIIKFDGKVDIDNADNIGFLIEHPKLYDNKSGLYNLKLFAQVLGKGFDKAYTDK
                     IIDAFGMRPYIKKKVKKYSMGMKQKLAIAVSLMNKPKFLILDEPTNGMDPDGSIDVLT
                     TIKSLVNELDMRILISSHKLEDIELICDRAVFLRDGHFVQDVNMEEGVASDTTIVTVD
                     HKDFDRTEKYLAEHFQLQNVDKADGHLMINAQKNYQVILKALSELDIYPKYIETRKSS
                     LRDTYFNINQRGDK"
     gene            3280..>3998
                     /gene="pmtD"
                     /locus_tag="ACGEXS_002373"
     CDS             3280..>3998
                     /gene="pmtD"
                     /locus_tag="ACGEXS_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtD"
                     /protein_id="HFF8013357.1"
                     /translation="MRILNLVKYDFYSIFKSPLTYLAILVVSSLIATQSILMANSMDN
                     PKHIIVYGSVFAAAKWLLLIIGLMFVVKTITRDFSQGTIQLYMSKVKTRVGYIISKTI
                     SIILISILFALIHYVILIVVQASSNGKNLAFSKYVDNLWFFLIFLLFFGLFLFLITLA
                     SQKTAMIFSLGVFLVLIVPFIKPFITFIPRYGEKVLDAFDYIPFAYLTDKMISSNFDF
                     SNWQWVISLGSIVIFFILNIL"
BASE COUNT         1413 a          487 c          672 g         1426 t
ORIGIN      
        1 tcgatgttga ggtgaaataa attgaaacat ctaacaaaaa tatttgtagt acttgcaatt
       61 attttattta ttattggtta ttatttacaa gcaactaatc atgaaagcca aggtataaaa
      121 ttattattag cagcgattat gtttatgatt tgtgctttta taagtagaag taatgatcga
      181 cgtaaaaata gtaaatagaa ttgactgttt aaaacgattg tttatgttga cgctacggtt
      241 gtcacgtaga gattaaagac attcgtttta gcagtctttt ttattgaagt agacaatgca
      301 agattaaatg gtagtgtcat ttcattttat tcaactgcta gtaatacaat actcagtgtt
      361 atttctgaag actggttgat aatgagacat gcatatttac aaaactgtgt atattgtgta
      421 tattgtatat atacagaagt tgataggggg acgttgatga aaataatttt aaaaaacaat
      481 agtgattttc cgatttatga acagattaag caacaagtaa aacaaaatat tttaaaggga
      541 catgttgctc ctggagagca tttgccgtca atgagagaac ttgccaaaga tcttcaagta
      601 agtttgatta ctaccaaacg tgcttatgaa gatttagaga aagacggttt tgttacaaca
      661 attagaggaa aagggacctt tgttaaggag caagatagtt ctattttaaa agagaaacaa
      721 ttttttacca ttgaaaattt ggttaaagaa ttggttaatg aagcgcaagc catcgaaatg
      781 tcacttgagg aactgcagga tattttaacg ttcatttatg aggaggaatc atcatgaatg
      841 ccatagaatt aagtaatgtt aattattcaa gtgatcagtt taatttaaag aatatctctt
      901 tcaaagttcc tcaaggattc gtcactggtt ttattggtag aaatggcgct ggtaaaacca
      961 caataataag gttaattatg gatttgtatc aaccacaaac tggtgttatt agagtattag
     1021 aagaggatat ggctctcaat ccgatagagt taaagaatag aattgggttt gtttactctg
     1081 aaaattattt taatgagaga tggactacga agcaacttga aaaaatgatt gccccttttt
     1141 atcgtaaatg ggaccatcaa gtatttgaat tttatcttga aaagttcgat ttaccaatca
     1201 ataaatcaat aaaaacattt tcgactggga tgaaaatgaa attatcacta gcagttgcat
     1261 tttcacatca tgctgaattg tatatttttg atgagcctac ttcaggtctt gatcccttag
     1321 ctagaaatga gctattggag ataattcagc aagaattaat tgatgaaaat aaaacaatat
     1381 tcatgtcgac acacattatc tcagatttag aaaaaatagc tgattatatc attcacttaa
     1441 gtgacggtga agttatctta aatggttcaa aagaacaact gttacaaaga tatcaagttg
     1501 tgagtggtgc tattgaggac cttgatgatg aattagcatc tctacttatt tatgaagaac
     1561 ataagcgtac gggatttata ggattaacag aacatgcgca agtatttaaa gaaattttag
     1621 gtcataaagt gaatatcact acaccttcta ttgagaactt aatggtttat cttgaaaaga
     1681 gaaaacctaa atatcatgaa aacatcaaat tgatggaaga aggtttttaa atgaaggcat
     1741 tgttaattag gaattttaaa ttacgacgat atacacttat catctatgtt ctattattaa
     1801 ccttatatcc tttttatata atgcttgact caacaaagtt cttttactta ttgcaatctt
     1861 ttataagtcc aacaatattg attatttgga ttttagatgc tggtcactta tttagactca
     1921 atcgtagatt aggtggtaat gattcatatt atttttatat gagtttacca gtgtcaaaaa
     1981 aacaattgct taatgccaac tatattacat gcattgtttt aacattaatt ggaacgcttg
     2041 ttattagttt atatgcttat gaagcagatg tgattgaacc aaattcaatc tatttttcaa
     2101 ctgcatatgc atttgtcata tctaacttct tgtctatacc aattgcattt agtcaattta
     2161 cagaattgcg tagagtcaaa gtgccatatg gtatatacgt gtttactatt atcattttag
     2221 ttccattttt attttcaatt gcaatagtat tggtgaatta ttttgttcta agtcaatcat
     2281 cattcccaga tttatattca tatattttaa atattggttt tctaattata agcattgtta
     2341 tacttattgt taattatttt aaacagctca ataaaataaa tactagaaaa tttaaaggag
     2401 gcagtcgatg aaattagaac atattacaaa aaaatacggc tcaaatgtcg ttttaaatga
     2461 tattgatttt gactttggcg atagtagaat tgtcggatta ataggaaaaa acggtgttgg
     2521 taaaacaacc gttatgaaag taatgaatgg taatattatt aaatttgatg gaaaagtaga
     2581 tattgataat gcagataata tcggtttttt aattgagcat cctaaattat atgataataa
     2641 atcaggattg tataacttga aattatttgc acaagtatta ggtaagggtt ttgataaagc
     2701 atacacagac aaaattatag atgcatttgg tatgagacct tatattaaaa agaaagttaa
     2761 gaaatattca atggggatga agcaaaagtt agcaattgca gtatctttaa tgaataaacc
     2821 taaattttta atcttggatg agcctacaaa tggtatggat ccagatggct caattgatgt
     2881 gctgactaca attaagtctt tagtaaatga acttgatatg agaattctaa tatcaagtca
     2941 taagttagaa gatattgaat taatttgtga tagagctgta tttttaagag acggacattt
     3001 tgttcaagat gtaaacatgg aggaaggtgt tgcatctgac acaacgatag ttactgttga
     3061 tcataaagac tttgatagaa ctgaaaaata tcttgcagag catttccaat tacaaaatgt
     3121 cgacaaagca gacggacatt taatgatcaa tgcacaaaaa aattatcaag ttatactaaa
     3181 agcattatct gaattagata tttatccgaa atatattgaa acacgtaaaa gttcattgcg
     3241 tgatacgtac ttcaatataa atcaaagagg tgataaataa tgagaatttt aaatttagtt
     3301 aagtatgatt tttatagtat atttaaaagt cctttaacat atttagcgat actagtcgta
     3361 tctagtttga ttgcaactca aagtatactt atggcaaatt cgatggataa cccgaaacat
     3421 attattgtct atggatctgt atttgctgca gcaaaatggt tattgttaat aattggatta
     3481 atgtttgttg ttaagacaat tacgcgtgat ttttcacaag gtacaattca actatatatg
     3541 agtaaagtta aaacacgcgt tggatacatt atttcgaaaa caatttcaat tattttaatt
     3601 tcaatattat ttgcattaat tcattatgtg attttgattg ttgtgcaggc atctagtaat
     3661 ggaaaaaatt tggcgttttc taaatatgta gataatttat ggttcttcct aatcttttta
     3721 ctattctttg gcttgttttt attcttaatc acacttgcat cacaaaaaac agcaatgata
     3781 ttttcattag gtgtattttt agtactcatt gtaccgttta ttaaaccttt tattacattt
     3841 atcccaagat acggtgaaaa agttttagat gcttttgatt atatcccttt tgcttactta
     3901 actgataaaa tgattagctc taactttgat tttagcaatt ggcaatgggt aatttcatta
     3961 ggttctatag tgatattctt cattttgaat atcttata
//
LOCUS       DAXWFK010000186         3940 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.187, whole genome shotgun sequence.
ACCESSION   DAXWFK010000186 DAXWFK010000000
VERSION     DAXWFK010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.187"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            6..815
                     /locus_tag="ACGEXS_002374"
     CDS             6..815
                     /locus_tag="ACGEXS_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFF8013358.1"
                     /translation="MCGLRSITLGTTNIEQTKHFIVDILGLNYEELLENSIRFGDADI
                     SPGTRLQFIQVPSEQLEESHFVGIGLRTPTDSGLEEYAEILSNKDIPFTTVKELNGNK
                     YFSLEDNNGHIFSIYSNENNYGVGLGMPSFESAVNPLHQVQGLGPVILKVNHVDITGQ
                     ILTNIFGLEVFAEYQPFDNADYHVQVFKVGTGGLGGEIHLMPVETEMTMPEYGAVDQV
                     EFETKDADFFNQAKSRLDEVEIPYQTLEQDDIESIRITENSGLSFIFTLQK"
     gene            844..1047
                     /locus_tag="ACGEXS_002375"
     CDS             844..1047
                     /locus_tag="ACGEXS_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6501 family protein"
                     /protein_id="HFF8013359.1"
                     /translation="MLHETWKERTPIKKVEVINTDAKKFTVSDMLTVGKQYDVINETE
                     EYYQIIDNSGLVGGYYKTYFKEV"
     gene            1228..2019
                     /locus_tag="ACGEXS_002376"
     CDS             1228..2019
                     /locus_tag="ACGEXS_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8013360.1"
                     /translation="MALKHYKNSDSTVFNDAKALFDLNKNILLKGPTGSGKTKLAETL
                     SEVVDTPMHQVNCSVDLDTESLLGFKTIKTNAEGQQEIVFVDGPVIKAMKEGHILYID
                     EINMAKPETLPVLNGVLDYRRQITNPYTGEVIKAVPGFNVIAAINEGYVGTLPMNEAL
                     KNRFVVIHVDYIDGDILKNVIKEQSLLQDDKQIEQIIKFNEDLRTMSKQGQISEEAAS
                     IRALLDLCDLITVMPVERAIKRTIIDKLEDEREQQAIYNAVELNF"
     gene            2033..3919
                     /locus_tag="ACGEXS_002377"
     CDS             2033..3919
                     /locus_tag="ACGEXS_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013361.1"
                     /translation="MSDRFIKFNDEQLDAKQVMMLQDLARLLLKNEQTQVKIQKFPYY
                     NPVQNVLITSWFWSHRPSHIEMAGLKTDVMLAAYGYHMMDVQIVNEVVQDKTFKHPKF
                     YQQLFKLLEDMRVLNSIKVERPSTAKLIDLRLDTRISYTESQIKVYRTKTQYTDLLFL
                     YLEHAFLSQDFFDIPSIHSDLDDILVNMFLYLPNFFQNQNSEDNMYLAQRIMYQVDDI
                     LKEDMLNEYYYLPKTLYNTLASPEFDDLKRTDASQVDGQDDTSEDDDNESEKADSKSA
                     DSESKGGAYLEMELHEGQNSETLGNDEAREGDATDDMTDMMTKKGKGSNDTLNREEGD
                     AVGQSQAFQLDGVNKNVEIKWQIPEIEPQYVLEYQESKQDVQYEIKDLIQIIKKTIER
                     EQRDARFNLTKGRLQKDLINWFIDDQYKLFYKKQDLSKSFDATFTLLIDASASMHDKM
                     AETKKGVVLFHETLKALNIKHEILSFSEDAFDSDEHAQPNIINEIINYDYSTFEKDGP
                     RIMALEPQDDNRDGVAIRVASERLMRRNQHQRFLIVFSDGEPSAFNYSQDGIIDTYEA
                     VEMSRKFGIEVFNVFLSQDPITEDVEQTIHNIYGQYAIFVEGVAHLPGHLSPLLKKLL
                     LKSL"
BASE COUNT         1480 a          542 c          710 g         1208 t
ORIGIN      
        1 ctataatgtg tggacttaga agtataacat taggtacaac aaatatagaa cagacaaaac
       61 atttcatagt tgacatatta ggattaaatt atgaagaact tcttgaaaac tcaattcgtt
      121 tcggcgatgc agatataagc ccaggaacaa gacttcaatt tatacaagtt ccaagtgagc
      181 aattagaaga atctcacttt gtgggtattg gattacgtac accaactgac tcaggtttag
      241 aggagtatgc ggaaatatta tcgaataagg atattccatt tacaacagtt aaagaattaa
      301 atggcaataa atatttcagt ctcgaagata acaatggtca tattttctca atatattcaa
      361 acgagaataa ttatggcgtt ggtttaggta tgccttcttt tgagagtgcg gtcaatccgt
      421 tacatcaagt gcaaggttta ggaccagtga ttcttaaagt gaatcatgta gatattacag
      481 gtcaaatttt aacaaatata ttcggacttg aagtatttgc agaataccaa cccttcgaca
      541 atgctgacta tcatgtccaa gtattcaaag ttggaacggg tggtctaggt ggcgaaatac
      601 atttaatgcc tgtagaaacc gaaatgacaa tgccggaata tggcgcagtc gatcaagttg
      661 agtttgaaac gaaagatgca gatttcttta atcaagcgaa atcgcgctta gatgaagtgg
      721 aaataccata tcaaacgctt gagcaagatg atattgaatc aattagaatt actgaaaaca
      781 gtggattatc gtttatattc actttacaaa aataattttt ttacgatagc gaggacaaaa
      841 tttatgttac atgaaacttg gaaagaacgt acaccaatca agaaagtaga agtcattaat
      901 acagatgcaa agaaatttac agtttctgac atgttaacgg ttggcaaaca atatgatgtt
      961 atcaacgaaa ctgaagaata ttatcaaata atagataatt ctggattagt tggcggttac
     1021 tataaaacat atttcaaaga agtttaaacg cataattatt gaaatgaagc ctaatatgag
     1081 attttcaatt agtaaatttg gtaaaatata aacaattcgc atactaagca ttgcgtttta
     1141 tatcagaata gaatagtaaa ctaaggcatt tggagaactt attccatgtc ttagtttttt
     1201 aatttattta aaggagaacg ttttaaaatg gcactaaaac attataagaa ttcagattca
     1261 acagttttca atgatgcgaa ggcattattt gatttaaata aaaatatttt acttaaaggt
     1321 ccaacaggtt cagggaaaac aaagttggca gaaacattaa gtgaagttgt tgatacaccc
     1381 atgcatcaag tcaattgttc tgttgattta gatacagaaa gcttattagg ctttaaaaca
     1441 attaaaacaa atgcggaagg tcaacaagaa attgtctttg tagatggtcc agttattaaa
     1501 gctatgaaag aggggcatat tttatatatt gatgaaataa atatggctaa acctgaaaca
     1561 ttgcctgtat taaatggggt cttagattat cgtcgtcaaa ttacgaatcc atacactggt
     1621 gaagtaatca aagctgtacc aggatttaac gttatagcag cgataaatga aggttatgtt
     1681 ggtactttgc caatgaatga agcactaaaa aatcgctttg ttgttattca cgttgattat
     1741 attgatgggg acattttaaa aaatgtgatt aaagagcaaa gtttattaca agatgataaa
     1801 caaatcgaac aaattattaa gtttaatgaa gatttacgta ctatgtctaa gcagggacaa
     1861 atttctgaag aagccgctag tatccgtgca ttattagact tgtgtgattt aatcactgta
     1921 atgccagttg aacgtgcaat taaacgtaca attattgata aattggaaga tgaacgtgaa
     1981 caacaagcaa tatataatgc tgtagaacta aacttttaaa gagggataaa acatgagtga
     2041 tcgtttcata aaatttaatg acgaacagtt agatgccaag caagtaatga tgttgcaaga
     2101 cttagctcga ctcttattaa aaaatgaaca aacacaagtt aaaatccaaa aattccctta
     2161 ctataatcct gttcaaaatg ttttgattac gagttggttt tggtcacatc gaccaagcca
     2221 tatcgaaatg gctggtttaa aaacagatgt tatgttagct gcttacggtt atcatatgat
     2281 ggatgtacaa attgtaaatg aagtagtaca agataaaact tttaaacacc ctaaatttta
     2341 tcaacaactt tttaaattat tagaagatat gcgcgtatta aattctatta aagttgagcg
     2401 cccaagtact gctaaattaa ttgatcttcg cttagataca cgtatatcat atacggaatc
     2461 tcaaattaaa gtgtatcgta ctaaaacaca atatacagat ttattatttt tgtatttaga
     2521 acatgcattt ttaagccaag atttctttga tataccatct atacattcag atttagatga
     2581 tattttagta aatatgttct tatatttacc taattttttc caaaatcaaa attctgaaga
     2641 taatatgtat ctggctcaaa gaattatgta tcaagtagat gatattttaa aagaagatat
     2701 gctaaacgaa tattattact tacctaaaac actatataat acattagcta gtccagagtt
     2761 tgatgattta aagagaactg atgcaagtca agtcgatggg caagatgata cttcagaaga
     2821 tgatgataac gaaagtgaaa aagctgactc taaatctgca gatagtgagt caaaaggtgg
     2881 cgcctattta gaaatggagc ttcacgaagg acaaaatagt gaaacattag gtaatgatga
     2941 agcacgtgaa ggcgatgcta cagatgatat gactgatatg atgaccaaaa agggcaaagg
     3001 atcaaacgat acgctaaatc gtgaagaagg cgatgctgtt ggtcaaagcc aagcatttca
     3061 acttgatggt gtgaacaaaa atgtagaaat aaaatggcaa attcctgaaa ttgagccaca
     3121 atatgtttta gaatatcaag aatcgaaaca agacgtacaa tatgaaatca aagacttaat
     3181 tcaaattata aagaaaacga ttgaacgaga acaacgagat gcacgtttta atttaacaaa
     3241 agggcgatta caaaaggatt taattaattg gtttatcgac gatcaatata aattgtttta
     3301 caaaaaacaa gatttaagta aatcatttga tgcgacattt acattgttaa ttgatgcgtc
     3361 agcaagtatg catgataaaa tggctgaaac gaagaaaggt gtagtgttat tccacgagac
     3421 gttaaaagca ttgaacatta aacatgaaat tttatcattc agtgaggatg cctttgattc
     3481 agacgagcat gcacaaccaa atatcattaa tgaaattatt aactatgatt actcaacctt
     3541 tgaaaaagat ggcccacgta ttatggcact tgaacctcaa gatgataatc gtgatggtgt
     3601 tgctattaga gttgcaagtg aacgattaat gcgtcgaaat caacatcaac gatttttaat
     3661 cgtattttca gacggcgaac cgtctgcatt taattatagt caagatggta ttattgatac
     3721 gtatgaagct gtagaaatgt cacgtaaatt tggtattgaa gtatttaatg tatttttgag
     3781 tcaagatcca ataactgaag atgttgaaca aacaattcat aatatttatg gtcaatatgc
     3841 tattttcgtt gaaggtgttg cgcatttacc tggacatctc tctccattat taaaaaaatt
     3901 actacttaaa tctttataaa taaatttaaa atatgagttt
//
LOCUS       DAXWFK010000187         3752 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.188, whole genome shotgun sequence.
ACCESSION   DAXWFK010000187 DAXWFK010000000
VERSION     DAXWFK010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.188"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..534
                     /gene="mroQ"
                     /locus_tag="ACGEXS_002378"
     CDS             <1..534
                     /gene="mroQ"
                     /locus_tag="ACGEXS_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic endopeptidase
                     MroQ"
                     /protein_id="HFF8013362.1"
                     /translation="ELEVKEPKKYIIPWALLGFALVMIYQMVVSIVLTQIYGGQQVSP
                     NTEKLIIIARKIPIFIFFVSIIGPLLEEYVFRKVIFGELFNAIKGNRIVAFIIATTVS
                     SLIFALAHNDFKFIPVYFGMGVIFSLAYVWTKRLAVPIIIHMLQNGFVVIFQLLNPEA
                     LKKATEQANFIYHIFIP"
     gene            complement(559..1740)
                     /locus_tag="ACGEXS_002379"
     CDS             complement(559..1740)
                     /locus_tag="ACGEXS_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrH family protein"
                     /protein_id="HFF8013363.1"
                     /translation="MSYHWFKKMLLSTSILILSSSSLGLATHTVEAKDNLNGEKPTTN
                     LNHNITSPSVNSEMNNNETGTPHESNQTGNEGTGSNSRDANPDSNNVKPDSNNQNPST
                     DSKPDPNNQNPSPNPKPDPDNPKPKPDPKPDPDKPKPNPDPKPDPDKPKPNPNPKPDP
                     NKPNPNPSPDPDQPGDSNHSGGSKNGGTWNPNASDGSNQGQWQPNGNQGNSQNPTGND
                     FVSQRFLALANGAYKYNPYILNQINKLGKDYGEVTDEDIYNIIRKQNFSGNAYLNGLQ
                     QQSNYFRFQYFNPLKSERYYRNLDEQVLALITGEIGSMPDLKKPEDKPDSKQRSFEPH
                     EKDDFTVVKKQEDNKKSASTAYSKSWLAIVCSMMVVFSIMLFLFVKRNKKKNKNESQR
                     R"
     gene            1937..2563
                     /locus_tag="ACGEXS_002380"
     CDS             1937..2563
                     /locus_tag="ACGEXS_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFF8013364.1"
                     /translation="MGLFKKDKKAMTFDNAMEERRSIYNLKDSISISDDELESMIAHA
                     VKHVPSSFNSQSTRIVLLLNDNNNKFWDNTKAILKEVMGENRDFEPTEQKIDNFKHSY
                     GTILFYEDQDVVSGLQEQMPNYYDNFAIWSTQTNAMHQFAIWTALATKGIGASLQHYN
                     PLVDEMTSNEFNIPKSWKLIAQMPFGDIREAAGEKTFNPVEDRFVIKK"
     gene            2924..3709
                     /locus_tag="ACGEXS_002381"
     CDS             2924..3709
                     /locus_tag="ACGEXS_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen family hydrolase"
                     /protein_id="HFF8013365.1"
                     /translation="MKVQIYQLPIVFGDSSKNETQITQWFEKNMNAEVDVVVLPEMWN
                     NGYDLEHLNEKADNNLGQSFSFIKHLAEKYKVDIVAGSVSNIRNNQIFNTAFSVNKSG
                     QLINEYDKVHLVPMLREHEFLTAGEYVAEPFQLSDGTYVTQLICYDLRFPELLRYPAR
                     SGAKIAFYVAQWPMSRLQHWHSLLKARAIENNMFVIGTNSTGFDGNTEYAGHSIVINP
                     NGDLVGELNESADILTVDLNLNEVEQQRENIPVFKSIKLDLYK"
BASE COUNT         1200 a          564 c          652 g         1336 t
ORIGIN      
        1 gaattagaag ttaaagaacc taaaaaatat atcattccat gggcattgct tggatttgca
       61 ttggtaatga tttatcaaat ggtagtgagc attgtattaa cgcaaattta tggtggacaa
      121 caagtaagtc ctaatacaga aaagctaatt attattgctc gaaaaatacc tatatttatc
      181 ttctttgtat ctattattgg tcctttatta gaagaatatg tattcagaaa agtaatcttt
      241 ggagaattat ttaatgcgat taaaggtaat cgtatcgtgg catttattat tgctacaaca
      301 gtaagttcat taatatttgc attagcacat aatgatttca aatttattcc agtttatttt
      361 ggtatgggtg tcattttttc attagcatat gtttggacaa aacggcttgc tgttccaatt
      421 attatccata tgttacaaaa cggatttgtc gttatattcc aattacttaa tcctgaggct
      481 ttgaaaaaag ccacggaaca agcgaatttt atatatcaca tttttattcc ataataaaaa
      541 aaacgaatca cacacggatt atcgtcgctg tgattcgttt ttatttttct ttttatttcg
      601 ctttacaaat aagaatagca tgattgaaaa taccaccatc atagaacata caattgctag
      661 ccaactttta ctatatgcag ttgacgcact tttcttatta tcttcttgtt ttttaactac
      721 tgtaaaatcg tctttttcat gcggttcaaa tgagcgttgt tttgaatccg gcttatcttc
      781 gggctttttc aaatctggca ttgatccaat ttcaccagta attaatgcga gtacttgttc
      841 atctaaatta cgatagtacc tttctgattt caatggattg aaatattgga atctaaagta
      901 attcgattgc tgttgtaatc catttaaata tgcatttccg ctgaaatttt gttttcgaat
      961 aatattataa atgtcttcat cagtaacttc tccataatct ttgcccaact tattaatttg
     1021 atttaaaata tacggattat acttgtaagc cccatttgcc aaggctaaaa atcgttggga
     1081 tacaaaatca ttaccagtag gattttgtga gtttccttga ttcccatttg gttgccattg
     1141 accttgatta gatccatctg aagcatttgg gttccatgtc cccccatttt tcgagccacc
     1201 agaatgattg gaatccccag gttgatcggg atctggtgac gggttaggat ttggcttatt
     1261 agggtctggt tttggattcg gatttggctt tggtttatct ggatctggtt ttggatccgg
     1321 atttggcttt ggtttatctg ggtctggttt tggatccggt tttggtttcg ggttatctgg
     1381 atctggttta ggattcggac ttgggttttg gttatttggg tctggttttg aatctgtact
     1441 tgggttttgg ttgtttgagt ctggcttcac attattcgaa tcaggattag catcacgact
     1501 attcgaacct gttccttcat tacccgtttg atttgattcg tgaggtgtcc cagtctcatt
     1561 attattcatt tcactattta ctgatggtga agttatatta tgattcaaat tagtagttgg
     1621 tttttctcca tttaagttat cctttgcttc aactgtgtgc gttgcaagcc ctaaactact
     1681 actacttaaa attaaaatac ttgttgaaag taacattttc ttaaaccaat gatatgacat
     1741 ttcacgagac acctcccttt ttcatgtaag tattaatcta agtataataa atatgcgttg
     1801 aaaatgcatt tatatttgga atttgaaaat gaatattaat attcttgaaa ttttcgtaaa
     1861 tattgcttta caagtacgag tataaatttt atacttagtc tattaagtat tctttactaa
     1921 agaaggagca ataattatgg gcttatttaa aaaagataaa aaagcaatga cttttgacaa
     1981 tgcaatggaa gaaagacgtt caatatataa tttgaaagat tcgatatcaa ttagcgatga
     2041 cgaattagaa tcaatgattg cacatgcagt taaacatgtt ccttcatcat ttaattctca
     2101 atcaactaga attgttctct tgttaaatga caacaataat aaattttggg ataacacaaa
     2161 agcaatttta aaagaagtca tgggagagaa tcgtgatttc gagccaactg aacaaaaaat
     2221 tgataacttc aaacattcat acggtacaat tttgttttat gaagatcaag atgttgtaag
     2281 tggtttacaa gaacaaatgc caaactacta tgataatttt gcaatatggt caactcaaac
     2341 aaatgccatg catcaatttg caatttggac tgcattagct actaaaggca taggtgcttc
     2401 attacaacac tataatccac ttgttgatga gatgacttca aatgaattta atattcctaa
     2461 aagttggaaa cttattgctc aaatgccatt tggtgatatt cgtgaagctg ctggcgaaaa
     2521 aacatttaat ccagtggaag atcgtttcgt aataaaaaaa taatcaatat attgttagga
     2581 atgggacaga aatgataaag aatcattagt tattcattat gtagtagttc ttacacatta
     2641 gccgctgcta atgtgcactt aagaatagga atacatgagt aaaactcatg tataagaaat
     2701 actaatttct aaagaaaaag tatttcttta tgttagagcc caccccaact tgcattgtct
     2761 gtagaaattg gtgttccaat ttctctatgt tggggcccca tcaactactg tcaatataac
     2821 tttgtagagc ctaggacttt gattttatgt cttgtgctcg ttttttgcgt ttcaacataa
     2881 cttttagtaa gttatatata agatctatca aggatgtgat gttatgaaag tccaaattta
     2941 tcaattaccg attgtttttg gagatagttc taaaaatgaa actcaaataa cgcaatggtt
     3001 cgaaaaaaat atgaatgctg aagtagatgt agtcgtttta cctgaaatgt ggaataatgg
     3061 ctatgattta gagcatttaa atgaaaaagc agataataat ctaggacaaa gtttttcatt
     3121 tattaaacat ttagctgaaa aatataaagt cgatatcgta gctgggtcag tttctaacat
     3181 tagaaacaat caaatattta atacagcttt tagtgtgaat aaaagtggcc agctgattaa
     3241 tgaatacgac aaagttcatt tagttcctat gttaagagag catgaatttt taaccgctgg
     3301 cgaatacgta gctgaaccct ttcaattgtc tgacggtact tatgtgaccc aacttatctg
     3361 ttatgattta cgttttccag aattattaag atatcctgct cgtagtggtg ctaaaattgc
     3421 attttatgtg gcgcaatggc ctatgtcacg tcttcaacat tggcattcat tactaaaggc
     3481 acgtgcaatt gaaaataata tgtttgtcat tggaactaat agcactggat tcgatggtaa
     3541 cacagaatat gctggtcatt cgatagttat aaacccaaat ggagatttag ttggagaatt
     3601 aaatgaatcc gcagatattt tgactgtaga tttaaactta aatgaagtag aacagcaacg
     3661 cgaaaatata cctgtattta aaagtattaa attagatttg tataaataag aagcgcccga
     3721 aataatattt aacacatgtc atgactaaac at
//
LOCUS       DAXWFK010000188         3691 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.189, whole genome shotgun sequence.
ACCESSION   DAXWFK010000188 DAXWFK010000000
VERSION     DAXWFK010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.189"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            113..643
                     /locus_tag="ACGEXS_002382"
     CDS             113..643
                     /locus_tag="ACGEXS_002382"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8013366.1"
                     /translation="MQIRQIHQHDFAQVDQLIRTAFENSEHGYGNESELVDQIRLSDT
                     YDNTLELVAVLQNEVVGHGLLSEVYLDNEAQREIGLVLAPVSVDIHHQNKGIGKRLIQ
                     ALEREAILKGYNFISVLGWPTYYANLGYQRASMYDIYPPYDGIPDEAFLIKELKVNSL
                     AGKTGTINYTSAFEKI"
     gene            906..3689
                     /gene="clfA"
                     /locus_tag="ACGEXS_002383"
     CDS             906..3689
                     /gene="clfA"
                     /locus_tag="ACGEXS_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin clumping factor ClfA"
                     /protein_id="HFF8013367.1"
                     /translation="MNMKKKEKHAIRKKSIGVASVLVGTLIGFGLLSSKEADASENSV
                     TQSDSASNESKSNDSSSVSAAPKTDDTNVSDTKTSSNTNNGETSVAQNPAQQETTQSS
                     STNATTEETPVTGEATTTTTNQANTPATTQSSNTNAEELVNQTSNETTSNDTNTVSSV
                     NSPQNSTNAENVSTTQDTSTEATPSNNESAPQSTDASNKDVVNQAVNTSAPRMRAFSL
                     AAVAADAPAAGTDITNQLTNVTVGIDSGTTVYPHQAGYVKLNYGFSVPNSAVKGDTFK
                     ITVPKELNLNGVTSTAKVPPIMAGDQVLANGVIDSDGNVIYTFTDYVNTKDDVKATLT
                     MPAYIDPENVKKTGNVTLATGIGSTTANKTVLVDYEKYGKFYNLSIKGTIDQIDKTNN
                     TYRQTIYVNPSGDNVIAPVLTGNLKPNTDSNALIDQQNTSIKVYKVDNAADLSESYFV
                     NPENFEDVTNSVNITFPNPNQYKVEFNTPDDQITTPYIVVVNGHIDPNSKGDLALRST
                     LYGYNSNIIWRSMSWDNEVAFNNGSGSGDGIDKPVVPEQPDEPGEIEPIPEDSDSDPG
                     SDSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDSASDSDSASDSDSD
                     NDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSESDSDSDSDSDSDSDSDSDSDSDSASDSDSGSDSDSSSDSDSESDSNSD
                     SESGSNNNVVPPNSPKNGTNASNKNEAKDSKEPLPDTGSEDEANTSLIWGLLASIGSL
                     LLFRRKKENKDKK"
BASE COUNT         1324 a          630 c          744 g          993 t
ORIGIN      
        1 attgcgaaaa atatcattca attttaatga aataatggcg tcattactat aaaatattac
       61 tttatgttgt aatgcatttt tctataagat agaactaaaa ggaggggcaa agatgcaaat
      121 tagacaaata catcaacatg actttgctca agtggaccag ttaattagaa cggcatttga
      181 aaatagtgaa catggttatg gtaatgaatc agagctagta gaccaaattc gtctaagtga
      241 tacgtatgac aataccttag aattagtagc tgttcttcaa aatgaagttg tagggcacgg
      301 tttactaagt gaagtttatc ttgataacga ggcacaacgg gaaattggat tagtgttagc
      361 acctgtatct gttgatattc atcatcaaaa taaaggtatt gggaagcgat tgattcaagc
      421 attagaacga gaagcaatat taaaaggata taattttatc agtgtattag gatggccgac
      481 gtattatgcc aatctaggat atcaacgcgc aagtatgtac gacatttatc caccatatga
      541 tggtatacca gacgaagcgt ttttaattaa agaattaaaa gtgaacagtt tagcgggaaa
      601 aacaggtacc ataaattaca catctgcttt tgaaaaaata tgatttcaag ctaggattac
      661 attaggtaga gttcatatta ataataaaaa atgtttgcaa tcaaatcgta cgttgtcgtt
      721 tgtaattctt aaaatagcaa taaataaaat gtttgttagt aaagtattat tgtggataat
      781 aaaatatcga tacaaattaa ttgctataat gcaattttag tgtataattc cattaacaga
      841 gattaaatat atctttaaag ggtatatagt taatataaaa tgacttttta aaaagaggga
      901 ataaaatgaa tatgaagaaa aaagaaaaac acgcaattcg gaaaaaatcg attggcgtgg
      961 cttcagtgct tgtaggtacg ttaatcggtt ttggactact cagcagtaaa gaagcagatg
     1021 caagtgaaaa tagtgttacg caatctgata gcgcaagtaa cgaaagcaaa agtaatgatt
     1081 caagtagcgt tagtgctgca cctaaaacag acgacacaaa cgtgagtgat actaaaacat
     1141 cgtcaaacac taataatggc gaaacgagtg tggcgcaaaa tccagcacaa caggaaacga
     1201 cacaatcatc atcaacaaat gcaactacgg aagaaacgcc ggtaactggt gaagctacta
     1261 ctacgacaac gaatcaagct aatacaccgg caacaactca atcaagcaat acaaatgcgg
     1321 aggaattagt gaatcaaaca agtaatgaaa cgacttctaa tgatactaat acagtatcat
     1381 ctgtaaattc acctcaaaat tctacaaatg cggaaaatgt ttcaacaacg caagatactt
     1441 caactgaagc aacaccttca aacaatgaat cagctccaca gagtacagat gcaagtaata
     1501 aagatgtagt taatcaagcg gttaatacaa gtgcgcctag aatgagagca tttagtttag
     1561 cggcagtagc tgcagatgca ccggcagctg gcacagatat tacgaatcag ttgacgaatg
     1621 tgacagttgg tattgactct ggtacgactg tgtatccgca ccaagcaggt tatgtcaaac
     1681 tgaattatgg tttttcagtg cctaattctg ctgttaaagg tgacacattc aaaataactg
     1741 tacctaaaga attaaactta aatggtgtaa cttcaactgc taaagtgcca ccaattatgg
     1801 ctggagatca agtattggca aatggtgtaa tcgatagtga tggtaatgtt atttatacat
     1861 ttacagacta tgtaaatact aaagatgatg taaaagcaac tttgaccatg cccgcttata
     1921 ttgaccctga aaatgttaaa aagacaggta atgtgacatt ggctactggc ataggtagta
     1981 caacagcaaa caaaacagta ttagtagatt atgaaaaata tggtaagttt tataacttat
     2041 ctattaaagg tacaattgac caaatcgata aaacaaataa tacgtatcgt cagacaattt
     2101 atgtcaatcc aagtggagat aacgttattg cgccggtttt aacaggtaat ttaaaaccaa
     2161 atacggatag taatgcatta atagatcagc aaaatacaag tattaaagta tataaagtag
     2221 ataatgcagc tgatttatct gaaagttact ttgtgaatcc agaaaacttt gaggatgtca
     2281 ctaatagtgt gaatattaca ttcccaaatc caaatcaata taaagtagag tttaatacgc
     2341 ctgatgatca aattacaaca ccgtatatag tagttgttaa tggtcatatt gatccgaata
     2401 gcaaaggtga tttagcttta cgttcaactt tatatgggta taactcgaat ataatttggc
     2461 gctctatgtc atgggacaac gaagtagcat ttaataacgg atcaggttct ggtgacggta
     2521 tcgataaacc agttgttcct gaacaacctg atgagcctgg tgaaattgaa ccaattccag
     2581 aggattcaga ttctgaccca ggttcagatt ctggcagcga ttctaattca gatagcggtt
     2641 cagattcggg tagtgattct acatcagata gtggttcaga ttcagcgagt gattcagatt
     2701 cagcaagtga ttcagactca gcgagtgatt cagattcagc aagcgattcc gactcagcga
     2761 gcgattccga ctcagacaat gactcggatt cagatagcga ttctgactca gacagtgact
     2821 cagattccga cagtgactca gattcagata gcgattctga ctcagacagt gactcagatt
     2881 cagatagcga ttcagattca gatagcgatt cagattccga cagtgattcc gactcagaca
     2941 gcgattctga ctccgacagt gattccgact cagacagcga ttcagattcc gacagtgatt
     3001 ccgactcaga tagcgattcc gactcagata gcgactcaga ttcagacagc gattcagatt
     3061 cagacagcga ttcagattca gatagcgatt cagattccga cagtgactca gattccgaca
     3121 gtgactcgga ttcagatagc gattcagatt ccgacagtga ctcagattcc gacagtgact
     3181 cagactcaga cagtgattcg gattcagcga gtgattcgga ttcagatagt gattccgact
     3241 ccgacagtga ctcggattca gatagcgact cagactcgga tagcgactcg gattcagata
     3301 gcgattcgga ctcagatagc gattcagaat cagacagcga ttcagattca gacagcgact
     3361 cagacagtga ctcagattca gatagtgact cggattcagc gagtgattca gactcaggta
     3421 gtgactccga ttcatcaagt gattccgact cagaaagtga ttcaaatagc gattccgagt
     3481 caggttctaa caataatgta gttccgccta attcacctaa aaatggtact aatgcttcta
     3541 ataaaaatga ggctaaagat agtaaagaac cattaccaga tacaggttct gaagatgaag
     3601 caaatacgtc actaatttgg ggattattag catcaatagg ttcattacta cttttcagaa
     3661 gaaaaaaaga aaataaagat aagaaataag t
//
LOCUS       DAXWFK010000189         3688 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.190, whole genome shotgun sequence.
ACCESSION   DAXWFK010000189 DAXWFK010000000
VERSION     DAXWFK010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.190"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(39..1481)
                     /gene="lmrS"
                     /locus_tag="ACGEXS_002384"
     CDS             complement(39..1481)
                     /gene="lmrS"
                     /locus_tag="ACGEXS_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter LmrS"
                     /protein_id="HFF8013368.1"
                     /translation="MAKVELTTRRRNFIVAVMLISAFVAILNQTLLNTALPSIMRELN
                     INESTSQWLVTGFMLVNGVMIPLTAYLMDRIKTRPLYLAAMGTFLLGSIVAALAPNFG
                     VLMLARVIQAMGAGVLMPLMQFTLFTLFSKEHRGFAMGLAGLVIQFAPAIGPTVTGLI
                     IDQASWRVPFIIIVGIAILAFVFGLVSISSYNEVKYTKLDKRSVMYSTIGFGLMLYAF
                     SSAGDLGFTSPIVIGALILSMVIIYLFIRRQFNITNALLNLRVFKNRTFALCTISSMI
                     IMMSMVGPALLIPLYVQNSLSLSALLSGLVIMPGAIINGIMSVFTGKFYDKYGPRPLI
                     YTGFTILTITTIMLCFLHTDTSYTYLIVVYAIRMFSVSLLMMPINTTGINSLRNEEIS
                     HGTAIMNFGRVMAGSLGTALMVTLMSFGAKIFLSTSPSHLTATEIKQQSIAIGVDISF
                     AFVAVLVMAAYVIALFIREPKEIESNRRKF"
     gene            complement(1803..2276)
                     /gene="sepA"
                     /locus_tag="ACGEXS_002385"
     CDS             complement(1803..2276)
                     /gene="sepA"
                     /locus_tag="ACGEXS_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter SepA"
                     /protein_id="HFF8013369.1"
                     /translation="MIVNYLKHKFYNLLTTMIVLFIFVLSGAIFLTFLGFGLYGLSRI
                     LIYFRLGDFTYNRSMYDNLLYYGSYIIFGYFIIFAVEHLMDYFRKMLPENAYFRGATF
                     HLISYTVATTLFYFIIHLNYVYINIDFWVIMVIIGFLYVCKLQFYPESKNLNNRK"
     gene            complement(2375..>3688)
                     /gene="sdrM"
                     /locus_tag="ACGEXS_002386"
     CDS             complement(2375..>3688)
                     /gene="sdrM"
                     /locus_tag="ACGEXS_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter SdrM"
                     /protein_id="HFF8013370.1"
                     /translation="ALILIMFMSAIESSIISLALPTIKQDLNAGNLISLIFTAYFIAL
                     VIANPIVGELLSRFKIIYVAIAGLLLFSIGSFMCGLSTNFTMLIISRVIQGFGSGVLM
                     SLSQIVPKLAFEIPLRYKIMGIVGSVWGISSIIGPLLGGGILEFATWHWLFYINIPIA
                     IIAIILVIWTFHFPEEETVAKSKFDTKGLTLFYVFIGLIMFALLNQQLLLLNFLSFIL
                     AIVVAMCLFKVEKHVSSPFLPVVEFNRSITLVFITDLLTAICLMGFNLYIPVYLQEQL
                     GLSPLQSGLVIFPLSVAWITLNFNLHRIEAKLSRKVIYLLSFTLLLVSSIIISFGIKL
                     PVLIAFVLILAGLSFGYIYTKDSVIVQEETSPLQMKKMMSFYGLTKNLGASIGSTIMG
                     YLYAIQSGIFGPNLHNVLSAVAVISIGLIVLWVVFFKEQSSQSKE"
BASE COUNT         1405 a          664 c          480 g         1139 t
ORIGIN      
        1 agcctattat tttaaataga ccaactacta taattatttt aaaatttcct tctattactt
       61 tctatttctt taggttctct tataaaaagt gctatcacat aagctgccat aacaagcaca
      121 gctacaaaag caaatgagat atccacccct atagcaatgg attgctgttt aatttcagtt
      181 gcagttaaat gcgatggcga tgtagataaa aatatttttg caccaaaact cattaatgta
      241 accattaaag ctgtgcctag tgaaccagcc attacacgac caaagttcat aatagccgtg
      301 ccatgtgaga tttcttcatt tctcaaagaa ttaattcctg tagtatttat cggcatcatg
      361 agtaaagaaa ctgaaaacat tctaattgca tatacgacta ttaaatacgt ataagatgtg
      421 tctgtatgca agaaacacaa cataattgta gtaattgtta aaattgtaaa accagtataa
      481 atcaatggtc taggaccata cttatcataa aatttacctg taaaaactga cataatacca
      541 tttattattg caccaggcat gataacaagt cctgataaca aggcagataa agataaactg
      601 ttttgaacat atagcggtat aagcagcgca ggtccaacca ttgacatcat tataatcatt
      661 gaactaatcg tacataatgc aaatgttcta tttttaaaaa cccttaaatt taaaagtgca
      721 ttagtaatat taaattgtct acgtataaat aaatagataa taaccatact taatatcaac
      781 gcacctatta ctattggact tgtaaatcct aaatctcctg cactactaaa tgcgtatagc
      841 attaacccga acccaatagt tgaatacatt actgaacgct tatctaattt cgtatatttc
      901 acttcattgt aactcgagat tgaaaccaaa ccgaaaacaa aggcaagtat agcaattcct
      961 acaattataa taaatggaac tcgccaactc gcttgatcaa taattaatcc tgtaactgta
     1021 ggtcctattg ctggtgcaaa ttgaattact aaacctgcta gtcccattgc aaaacctcga
     1081 tgttctttac tgaacaatgt aaataacgta aattgcatta agggcataag tacgcctgca
     1141 cccatcgctt gaattacacg agctaacatt aaaactccaa aattcggagc taaggctgca
     1201 acaatagaac ctaataaaaa tgtccccatc gccgctaagt ataaaggtct agttttaatt
     1261 ctatccatta gatatgccgt cagaggtatc atgacgccat taacaagcat aaacccagta
     1321 actagccatt gcgatgtact ttcattgata tttaattctc tcattatact aggtaacgct
     1381 gtatttaata atgtttgatt taaaatagct acaaacgcac taatcaacat aacagcaact
     1441 atgaagtttc gtctccgggt tgttaattca actttagcca tcataattcc tcctttttaa
     1501 aacagatata cacttgtccc tttaatacgt atagccatga acataatggg agtagtactc
     1561 aaataataaa gaagcactac ataataaatc attattggtt cttacacatt aggcacagct
     1621 aatgtgtact taaaaactaa caatataaca ttgtagggcc taataattta ttttatgtct
     1681 cagactcata caatggttat ttaacttatt taccacaaat tgagcactga gactcactat
     1741 tatcgattct tctattgaac aaaaagaagt tggaacacat atgtgccctc aacttcagat
     1801 atctattttc tattatttaa atttttactt tcagggtaaa attgaagctt acaaacatat
     1861 aagaacccta taataaccat gatgacccaa aaatcgatat tgatatacac atagttcaga
     1921 tgaataataa aataaaataa cgttgttgca actgtgtaag aaatcaaatg aaaagtcgcg
     1981 cctctaaaat atgcattttc aggcaacatt tttctaaaat aatccattaa atgctcgact
     2041 gcaaatatga tgaagtaccc aaagattata taactgccat aatataatag gttatcgtac
     2101 atacttctat tatatgtaaa gtcacccaac ctaaaataaa taagtattct acttaatcca
     2161 tataaaccaa accctagaaa tgttaaaaaa atcgcaccag aaagtacaaa aataaagaga
     2221 acaatcattg tagttaacaa gttataaaac ttatgtttaa gataattcac aatcattggc
     2281 ctccttatta ataaaatatt gaacgattta tctagtatct aggaaattta ttatttctta
     2341 ctttaattac actacatcaa ttttaatcat aattctattc ttttgattga gatgactgtt
     2401 ctttaaaaaa tacgacccat aaaacaataa gcccaatgct aattacagca acagcactta
     2461 acacattgtg taagtttgga ccaaagattc ctgattgtat cgcataaaga tatcccatta
     2521 ttgttgaacc tattgatgcc ccaagatttt tagttaatcc ataaaatgac atcattttct
     2581 tcatttgtaa tgggctagtt tcctcttgga caatcacact atctttcgta taaatatatc
     2641 caaaacttaa tcctgccaaa attaacacaa atgctataag taccggcaat ttaataccaa
     2701 atgaaatgat aatactactt actagtagca atgtaaatga tagtaagtaa ataactttcc
     2761 ttgatagttt tgcttcaatt cgatgtaaat taaaattcaa tgtaatccaa gctacagata
     2821 aaggaaaaat aaccaatcca ctttgcaatg gagataatcc tagttgttct tgaaggtaga
     2881 ctggaatata taaattgaat cccattaaac aaatagctgt tagaaggtca gttataaaaa
     2941 ctaaagtaat cgaacgatta aattcaacca caggtaaaaa tggtgaagaa acatgttttt
     3001 ctactttaaa taaacacatt gccacaacga tagctaagat aaaactcaag aaatttaaaa
     3061 gtaacagctg ttggtttaat aaagcaaaca taatcaatcc aataaataca taaaatagcg
     3121 tgagcccttt agtatcaaat tttgatttag caactgtttc ttcctctgga aaatgaaatg
     3181 tccatataac taatataatg gcaatgatcg caatcggtat atttatgtag aataaccaat
     3241 gccatgttgc aaattctaat ataccaccac ctagaagtgg cccaatgata ctcgaaatac
     3301 cccaaacact gccaactatg cccataattt tatatcgtaa cggaatttca aatgctaact
     3361 ttggaacaat ttgcgataat gacatcaata cacctgatcc aaaaccttgt atcacgcgtg
     3421 atataattag catagtaaaa ttagtactta aaccacacat aaaactacct atactaaata
     3481 aaagtaatcc tgcaattgcc acataaataa ttttaaatct cgaaagtaat tcgcccacta
     3541 taggattggc aattactaat gcgataaaat acgctgtgaa aattaatgaa attaaatttc
     3601 ctgcatttaa atcttgttta attgtaggta atgcaagcga aataatagat gattcaatcg
     3661 ctgacataaa cataattaat attaaagc
//
LOCUS       DAXWFK010000190         3636 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.191, whole genome shotgun sequence.
ACCESSION   DAXWFK010000190 DAXWFK010000000
VERSION     DAXWFK010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.191"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            64..1590
                     /gene="spa"
                     /locus_tag="ACGEXS_002387"
     CDS             64..1590
                     /gene="spa"
                     /locus_tag="ACGEXS_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal protein A"
                     /protein_id="HFF8013371.1"
                     /translation="MKKKNIYSIRKLGVGIASVTLGTLLISGGVTPAANAAQHDEAQQ
                     NAFYQVLNMPNLNADQRNGFIQSLKDDPSQSANVLGEAQKLNDSQAPKADAQQNNFNK
                     DQQSAFYEILNMPNLNEAQRNGFIQSLKDDPSQSTNVLGEAKKLNESQAPKADNNFNK
                     EQQNAFYEILNMPNLNEEQRNGFIQSLKDDPSQSANLLSEAKKLNESQAPKADNKFNK
                     EQQNAFYEILHLPNLNEEQRNGFIQSLKDDPSQSANLLAEAKKLNDAQAPKADNKFNK
                     EQQNAFYEILHLPNLTEEQRNGFIQSLKDDPSVSKEILAEAKKLNDAQAPKEEDNNKP
                     GKEDNNKPGKEDNNKPGKEDNNKPGKEDGNKPGKEDNKKPGKEDGNKPGKEDNKKPGK
                     EDGNKPGKEDGNKPGKEDGNGVHVVKPGDTVNDIAKANGTTADKIAADNKLADKNMIK
                     PGQELVVDKKQPANHADANKAQALPETGEENPFIGTTVFGGLSLALGAALLAGRRREL
                     "
     gene            complement(1919..3511)
                     /locus_tag="ACGEXS_002388"
     CDS             complement(1919..3511)
                     /locus_tag="ACGEXS_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFF8013372.1"
                     /translation="MTLLTVNPFDNVGLSALVAAVPIILFLLCLTVFKMKGIYAALTT
                     LVVTLIVALFVFELPARVSAGAITEGVVAGIFPIGYIVLMAVWLYKVSIKTGQFSIIQ
                     DSIASISVDQRIQLLLIGFCFNAFLEGAAGFGVPIAICAVLLIQLGFEPLKAAMLCLI
                     ANGAAGAFGAIGLPVSIIDTFNLSGGVTTLDVARYSALTLPILNFIIPFVLVFIVDGM
                     KGIKEILPVILTVSGTYTGLQLLLTIFHGPELADIIPSLATMVVLAFVCRKFKPKNIF
                     RLEASEHKIQKRTPKEIVFAWSPFVILTAFVLVWSAPFFKKLFQPGGALESLVIKLPI
                     PNTVSDLSPKGIALRLDLIGATGTAILLTVIITILITKLKWKSAGALLVEAIKELWLP
                     ILTISAILAIAKVMTYGGLTVAIGQGIAKAGAIFPLFSPVLGWIGVFMTGSVVNNNTL
                     FAPIQATVAQQISTSGSLLVAANTAGGVAAKLISPQSIAIATAAVKKVGEESALLKMT
                     LKYSIIFVAFICVWTFILTLIF"
BASE COUNT         1372 a          709 c          568 g          987 t
ORIGIN      
        1 gtattgcaat acataattcg ttatattatg atgactttac aaatacatac agggggtatt
       61 aatttgaaaa agaaaaacat ttattcaatt cgtaaactag gtgtaggtat tgcatctgta
      121 actttaggta cattacttat atctggtggc gtaacacctg ctgcaaatgc tgcgcaacac
      181 gatgaagctc aacaaaatgc tttttatcaa gtcttaaata tgcctaactt aaatgctgat
      241 caacgcaatg gttttatcca aagccttaaa gatgatccaa gccaaagtgc taacgtttta
      301 ggtgaagctc aaaaacttaa tgactctcaa gctccaaaag ctgatgcgca acaaaataac
      361 ttcaacaaag atcaacaaag cgccttctat gaaatcttga acatgcctaa cttaaacgaa
      421 gcgcaacgta acggcttcat tcaaagtctt aaagacgacc caagccaaag cactaacgtt
      481 ttaggtgaag ctaaaaaatt aaacgaatct caagcaccga aagctgataa caatttcaac
      541 aaagaacaac aaaatgcttt ctatgaaatc ttgaatatgc ctaacttaaa cgaagaacaa
      601 cgcaatggtt tcatccaaag cttaaaagat gacccaagcc aaagtgctaa cctattgtca
      661 gaagctaaaa agttaaatga atctcaagca ccgaaagcgg ataacaaatt caacaaagaa
      721 caacaaaatg ctttctatga aatcttacat ttacctaact taaacgaaga acaacgcaat
      781 ggtttcatcc aaagcctaaa agatgaccca agccaaagcg ctaacctttt agcagaagct
      841 aaaaagctaa atgatgctca agcaccaaaa gctgacaaca aattcaacaa agaacaacaa
      901 aatgctttct atgaaatttt acatttacct aacttaactg aagaacaacg taacggcttc
      961 atccaaagcc ttaaagacga tccttcagtg agcaaagaaa ttttagcaga agctaaaaag
     1021 ctaaacgatg ctcaagcacc aaaagaggaa gacaataaca agcctggcaa agaagacaat
     1081 aacaagcctg gcaaagaaga caacaacaag cctggtaaag aagacaacaa caagcctggc
     1141 aaagaagacg gcaacaagcc tggtaaagaa gacaacaaaa aacctggtaa agaagatggc
     1201 aacaagcctg gtaaagaaga caacaaaaaa cctggtaaag aagacggcaa caagcctggc
     1261 aaagaagatg gcaacaaacc tggtaaagaa gatggtaacg gagtacatgt cgttaaacct
     1321 ggtgatacag taaatgacat tgcaaaagca aacggcacta ctgctgacaa aattgctgca
     1381 gataacaaat tagctgataa aaacatgatc aaacctggtc aagaacttgt tgttgataag
     1441 aagcaaccag caaaccatgc agatgctaac aaagctcaag cattaccaga aactggtgaa
     1501 gaaaatccat tcatcggtac aactgtattt ggtggattat cattagcctt aggtgcagcg
     1561 ttattagctg gacgtcgtcg cgaactataa aaacaaacaa tacacaacga tagatatcat
     1621 tttatccaaa ccaattttaa cttatatacg ttgattaaca cattcttatt tgaaatgata
     1681 agaatcatct aaatgcacga gcaacatctt ttgttgctca gtgcattttt tattttactt
     1741 acttttctaa acaacttctg aaacgcctca acactttcta ctctgattac atatatgaca
     1801 tttttaggca ttaaaaaatc gaactagaca agatgctcat tgcatttcgt actagttcga
     1861 ttcatgaata attagattta aaatgtcatt tgaatccaag tgacaacatt atttatattt
     1921 agaatattaa cgttagtata aacgtccaaa cacaaataaa agcaacaaat ataatactgt
     1981 attttaacgt catttttaat aatgcagatt cttcaccaac ttttttaaca gctgcagtcg
     2041 caatggcaat tgattgtggt gaaataagtt tcgctgctac accacctgca gtgttagctg
     2101 ccacaagtaa tgaaccgctt gttgaaattt gttgtgccac tgtcgcttga ataggtgcga
     2161 ataaagtatt gttatttaca actgaaccag tcataaacac accaatccaa cctaatactg
     2221 gagagaataa tgggaaaatt gctcccgctt tagcaatacc ttgtccaatt gctacagtca
     2281 aaccaccgta tgtcataact ttagcaatag ctaggatagc tgaaattgta aggatcggta
     2341 accataattc tttaattgct tcgaccaata aagcacctgc acttttccat tttaacttcg
     2401 taattaaaat tgtaataatt actgttaata aaatcgctgt cccagttgca ccaattaaat
     2461 cgagacgcaa cgcaattcct ttaggcgata aatcactcac agtatttgga attggcaatt
     2521 ttattactaa actttcaagt gcacctccag gttggaataa ttttttgaag aatggtgcac
     2581 tccatactaa tacaaaggca gttaaaatta cgaacggact ccaagcaaag acaatttctt
     2641 taggcgttcg tttttgaatt ttatgttcag acgcttccaa tctgaaaatg tttttcggtt
     2701 taaatttacg acaaacaaat gctaacacca ccattgttgc tagtgatgga ataatgtctg
     2761 ctagttctgg accatggaat attgttaata ataattgtaa tccagtatat gtaccactca
     2821 ctgttaaaat gacaggtaaa atttctttaa tacctttcat accatctaca atgaatacta
     2881 aaacaaatgg aataataaag tttaaaattg gaagtgttaa tgctgagtat ctcgcaacat
     2941 ctaatgttgt aacgcctcca cttaagttaa acgtatcaat aatactaact ggtaaaccaa
     3001 ttgcaccaaa ggcacccgcc gcaccattag caattaaaca taacatcgct gcttttaatg
     3061 gttcaaatcc aagttgaatt aataatactg cacaaatcgc aattggcaca ccaaatcctg
     3121 ctgcaccttc taaaaatgcg ttgaaacaaa atccaattaa taatagttgg attctttggt
     3181 ccactgaaat acttgcaata ctatcttgaa taatagaaaa ttgtcctgtt ttaatagaaa
     3241 ctttatataa ccaaactgcc attaaaacga tatatcctat tgggaaaata ccggcaacaa
     3301 cgccttctgt aatcgcacct gctgatacac gcgctggtaa ttcaaataca aataaagcca
     3361 caatcaatgt aacaaccaaa gttgtcaatg ctgcataaat gcctttcatt ttaaaaacgg
     3421 ttaagcataa taaaaataaa ataataggta ctgctgcaac taaggctgat aatccgacat
     3481 tatcgaatgg atttacagta agtagtgtca taatgactcc ctctctttat ataaaatatt
     3541 tatcattctg attaatctac aacctatttc aacttatatt ttgcgatgat cacatattta
     3601 aaatgtaaca ctcctatatg tgacaggcaa tcgaat
//
LOCUS       DAXWFK010000191         3636 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.192, whole genome shotgun sequence.
ACCESSION   DAXWFK010000191 DAXWFK010000000
VERSION     DAXWFK010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.192"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(100..1899)
                     /gene="dnaG"
                     /locus_tag="ACGEXS_002389"
     CDS             complement(100..1899)
                     /gene="dnaG"
                     /locus_tag="ACGEXS_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFF8013373.1"
                     /translation="MRIDQSIINEIKDKTDILDLVSEYVKLEKRGRNYIGLCPFHDEK
                     TPSFTVSEDKQICHCFGCKKGGNVFQFTQEIKDISFVEAVKELGDRVNVAVDIEATQS
                     NSNVQIASDDLQMIEMHELIQEFYYYALTKTVEGEQALTYLQERGFTDALIKERGIGF
                     APDSSHFCHDFLQKKGYDIELAYEAGLLSRNEENFSYYDRFRNRIMFPLKNAQGRIVG
                     YSGRTYTGQEPKYLNSPETPIFQKRKLLYNLDKARKSIRKLDEIVLLEGFMDVIKSDT
                     AGLKNVVATMGTQLSDEHITFIRKLTSNITLMFDGDFAGSEATLKTGQNLLQQGLNVF
                     VIQLPSGMDPDEYIGKYGNDAFTAFVKNDKKSFAHYKVSILKDEIAHNDLSYERYLKE
                     LSHDISLMKSSILQQKALNDVAPFFNVSPEQLANEIQFNQAPANYYPEDEYGGYIEPE
                     PIGMAQFDNLSRQEKAERAFLKHLMRDKDTFLNYYESVDKDNFTNQHFKYVFEVLHDF
                     YAENDQYNISDAVQYVNSNELRETLISLEQYNLNDEPYENEIDDYVNVINEKGQETIE
                     SLNHKLREATRIGDVELQKYYLQQIVAKNKERM"
     gene            complement(1960..2778)
                     /locus_tag="ACGEXS_002390"
     CDS             complement(1960..2778)
                     /locus_tag="ACGEXS_002390"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFF8013374.1"
                     /translation="MEKIKIIVASDSIGETAELVARAGISQFNPKQCKNELLRYPYIE
                     SFEDVDEVIQVAKDTNAIIVYTLIKPEMKQYMSEKVAEFQLKSVDIMGPLMDLLSASV
                     EEKPYNEPGIVHRLDDAYFKKIDAIEFAVKYDDGKDPKGLPKADIVLLGISRTSKTPL
                     SQYLAHKSYKVMNVPIVPEVTPPDGLYDINPKKCIALKISEEKLNRIRKERLKQLGLG
                     DTARYATEARIQEELNYFEEIVSEIGCPVIDVSQKAIEETANDIIHYIEQNKSK"
     gene            complement(2789..3412)
                     /locus_tag="ACGEXS_002391"
     CDS             complement(2789..3412)
                     /locus_tag="ACGEXS_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8013375.1"
                     /translation="MELSQRQERIIEIVKTKEPITGEQIADKLNLTRATLRPDLAILT
                     MSGFLEARPRVGYYYSGKSKGKFFNEKLRQFEVKDYMSQPVVLRENTTVYDAICTIFL
                     EDVSTLFIINEDNDFVGVCSRKDLLRASMIGADIHKVPISVNMTRMPNVTYLEESELV
                     IYAADRMIEKEIDSIPIVRKKDNQKYEVIGRISKTTIAKLLVALYKE"
BASE COUNT         1112 a          669 c          471 g         1384 t
ORIGIN      
        1 tatttatttt tcaatagtgt tgtttaaatg cggtaactat catttaccct tatacatttt
       61 gacataatca ttattcgtat tattctttaa aatcacatgc tacatgcgtt ctttattctt
      121 agcaacaatt tgctgtaaat agtatttttg taattctaca tcgccaatcc ttgtagcttc
      181 ccttaattta tgattcaatg actcaattgt ttcttgtcct ttttcattaa taacattgac
      241 ataatcatca atttcatttt catatggttc gtcattcaaa ttatattgtt ctaagctaat
      301 tagtgtttct ctcaactcat ttgaattaac atactgcaca gcatcactga tattatattg
      361 atcattttcc gcataaaaat catgtaagac ttcgaataca tatttaaaat gctgatttgt
      421 gaagttatcc ttatcaacac tttcataata atttaaaaat gtatctttat ctctcattaa
      481 atgttttaaa aatgctcgct ccgctttttc ttgacggctc aaattgtcaa attgtgccat
      541 accaattggc tcaggttcaa tgtaaccgcc atactcatct tctggataat aattggctgg
      601 tgcttgattg aattgtattt cgttagctaa ttgctcagga ctaacattga aaaatggtgc
      661 aacatcattt aaagcctttt gttgcaaaat cgatgatttc ataagcgaaa tatcatgact
      721 tagttctttc aaataacgtt catatgaaag gtcattatgt gcaatttcat cttttaatat
      781 actcacttta taatgtgcaa atgacttttt gtcatttttt acaaaagcag taaatgcatc
      841 gttgccatac ttaccaatgt attcatccgg atccatgcct gatggcaatt gtataacaaa
      901 tacatttagc ccttgctgta acaaattttg acctgtttta agtgttgctt cactacccgc
      961 aaaatcccca tcaaacatta atgttatatt tgatgttaac tttcgtataa aagtaatatg
     1021 ttcatctgac aactgtgtac ccattgttgc aacaacgttt ttcaagccag cagtatcaga
     1081 ttttataaca tccataaaac cttctagtaa tacgatttca tctaattttc taattgattt
     1141 acgcgcttta tctaagttgt ataacaactt tcttttttga aagataggtg tttcaggact
     1201 atttaagtat tttggttctt gaccggtata tgttcgacct gaatatccaa caattcttcc
     1261 ttgcgcattt ttcaaaggaa acataatacg atttcgaaat ctatcgtaat aactgaaatt
     1321 ttcttcgtta cgtgataata atccggcttc atatgctaat tcaatatcgt aacccttttt
     1381 ttgaagaaaa tcatgacaaa aatgtgagct atcgggtgca aagccaatgc ctcgctcttt
     1441 aataagcgca tctgtaaaac cacgttcttg taagtacgtt aatgcttgtt cgccttcgac
     1501 tgtctttgtt aaagcgtaat aataaaattc ttgtattaac tcatgcattt caatcatttg
     1561 taaatcatca gaagcaattt gaacatttga gttagattgt gttgcctcaa tatctacagc
     1621 aacattaact ctatcaccta attctttaac cgcttcaaca aatgatatgt ctttaatttc
     1681 ttgagtaaat tgaaaaacat tgccaccttt tttacaacca aaacaatgac aaatttgttt
     1741 atcttcagaa actgtaaatg aaggtgtctt ttcatcatga aaaggacaca aacctatata
     1801 attgcgtcct ctcttttcta attttacata ttcacttacc aagtctaaaa tgtcggtttt
     1861 atcttttatt tcattaatga tcgattgatc tattcgcaaa ttaaatcacc tattaagcat
     1921 tagttatact attataccta attttcgaca aaaatgaaat catttcgatt tattttgttc
     1981 aatataatgg attatatcgt ttgctgtttc ttcgattgct ttttgagaaa catcaatgac
     2041 aggacatcca atttcactta cgatttcttc aaagtaattc aattcttctt gaattcgtgc
     2101 ttctgttgca tatcgagctg tgtcacctag tcctaattgt tttagtcgct cttttctaat
     2161 gcgatttaat ttttcttcac ttattttaag tgcgatacat ttctttggat taatatcata
     2221 taagccatct ggcggtgtca cttctggtac aatcggtaca ttcataactt tgtaactctt
     2281 atgcgctaaa tactgagata atggtgtctt tgaagttctc gaaataccaa gtaaaacaat
     2341 atcagcttta ggtaatcctt taggatcttt accatcatca tatttaactg caaactctat
     2401 cgcatcaatt ttcttgaaat atgcatcatc taatctatga acgatacctg gctcattata
     2461 aggtttttct tcaaccgaag cagataataa atccattaat ggccccatga tatcgacaga
     2521 cttcaattgg aattctgcta ctttctcact catatattgc ttcatttcag gtttaataag
     2581 tgtataaaca atgatagcat ttgtatcttt tgcaacttga atcacttcat caacatcttc
     2641 aaaagattca atatatggat atcttaataa ttcattttta cattgcttag gattgaattg
     2701 tgaaatacct gccctagcaa ctaactctgc cgtttcacct atagaatctg aagctacgat
     2761 aattttaatt ttttccatta cttctcacct attctttata taatgctact aataacttag
     2821 ctattgttgt tttggaaatt cttccaatta cttcatactt ttgattatct ttttttctta
     2881 caattggaat cgaatcaatt tctttttcaa tcattctatc tgctgcgtat atgactaatt
     2941 cgctttcctc taaataagtg acattaggca tacgtgtcat atttacactg ataggtactt
     3001 tatgaatatc tgctccaatc attgaagctc ttaataaatc ttttcttgaa cacacaccaa
     3061 caaaatcgtt atcttcatta ataataaata atgtacttac atcttctaaa aaaattgtac
     3121 aaatagcgtc ataaactgtt gtattctctc ttagcacaac aggttgagac atatagtcct
     3181 taacctcaaa ttgtcgaagt ttttcattaa aaaatttgcc ttttgattta cctgaataat
     3241 aatatccaac tcggggacgc gcttctaaaa aacctgacat tgttaatatc gctaaatctg
     3301 gtctaagcgt tgctcttgtt aaattcaact tatctgctat ttgttcacca gtaatgggtt
     3361 ctttagtttt aacaatttcg atgattcgtt cttgtctttg actgagttct ataggtcttc
     3421 accccttttt tattcaccta ttattatacc gatatttctt tcatgtcaca aacacattaa
     3481 ttttacttgc ctttaaataa tctatcaatt agaataaacg tacagcgagt taaggatagt
     3541 gtaagcttaa caataagatt ggcgcaacga atcattttaa aataaaagcg agtgactaca
     3601 ctaatttggg tggaaccgcg ggttaaatac gtaact
//
LOCUS       DAXWFK010000192         3615 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.193, whole genome shotgun sequence.
ACCESSION   DAXWFK010000192 DAXWFK010000000
VERSION     DAXWFK010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.193"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(189..1097)
                     /locus_tag="ACGEXS_002392"
     CDS             complement(189..1097)
                     /locus_tag="ACGEXS_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8013376.1"
                     /translation="MKKLLLPLIIMLLVLAACGNQGEKNNKAETKSYKMDDGKTVDIP
                     KDPKRIAVVAPTYAGGLKKLGANIVAVNQQVDQSKVLKDKFKGVTKIGDGDVEKVAKE
                     KPDLIIVYSTDKDIKKYQKVAPTVVVDYNKHKYLEQQEMLGKIVGKEDKVKAWKKDWE
                     ETTAKDGKEIKKAIGQDATVSLFDEFDKKLYTYGDNWGRGGEVLYQAFGLKMQPEQQK
                     LTAKAGWAEVKQEEIEKYAGDYIVSTSEGKPTPGYESTNMWKNLKATKEGHIVKVDAG
                     TYWYNDPYTLDFMRKDLKEKLIKAAK"
     gene            complement(1312..2466)
                     /locus_tag="ACGEXS_002393"
     CDS             complement(1312..2466)
                     /locus_tag="ACGEXS_002393"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HFF8013377.1"
                     /translation="MNDMDNSFLITTEIQRKWIEKFKVIRDTFKAKAEYNDQHSQFPY
                     KNIEWLIKEGYGKLTLPKAYGGEGATIEDMVILQSFLGELDGATALSIGWHVSVVGQI
                     YEQKLWSQDMLEQFAVEINNGALVNRAVSEAEMGSPTRGGRPSTHAVKADDGYILNGV
                     KTYTSMSKALTHIIVAAYIEELESVGFFLVDRNLPGVEIADNWDVLGMRATESHDLIL
                     NDVKVPLKHLVETEKSKAPNGWILHIPSCYLGIAQAARNYAVDFAIQHSPNSIEGTIA
                     TLPTVQQNLGKMETLLLCARQFLWSTAKGYQQYKDDSQIRNPTSASKVMVMNQGLEVI
                     DLAMRIVGAKSLEMNRPLQRYYRDMRAGLHNPPMEDAAYTNIAKSITDTF"
     gene            complement(2683..3588)
                     /gene="yut"
                     /locus_tag="ACGEXS_002394"
     CDS             complement(2683..3588)
                     /gene="yut"
                     /locus_tag="ACGEXS_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016266007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015204 - urea transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HFF8013378.1"
                     /translation="MKIIDVLLKNISQVVLISNKWTGLFILIGLFVADWTIGLAAIVG
                     SIIAYTFARFINYSEAEINDGLAGFNPVLTAIALTIFLDKSGLDIVITMIATLLTLPV
                     AAAVREVLRPYKVPMLTMPFVIVTWFTILLSGQVKFVDTSLKLMPQNIETVNFSNNDR
                     IHFIQSLFEGFSQVFIEASVIGGVCILIGILIASRKATLLAVIASLLSFIIVALLGGN
                     YDDINQGLFGYNFVLMAIALGYTFKTAINPYISTFLGVLLTVVVQLGTTTLLEPFGLP
                     ALTLPFIIVTWILLFAGIKHDKVDA"
BASE COUNT         1144 a          727 c          432 g         1312 t
ORIGIN      
        1 ttctcaggta tactacttta gctataataa tactaatcta tcattaatta aattgaacga
       61 taacaacggc acttattatt taaaaaaata caaatatcta actttctagc tataaaaata
      121 agagcctaat acaatatcaa aagattgata tgtttcagac tctcatttca ctaacttata
      181 tagctgaatt attttgcagc tttaattaat ttttctttta aatctttacg catgaaatct
      241 aatgtataag gatcgttgta ccagtatgta ccagcatcaa ctttaacaat atgtccttct
      301 ttagtagctt tcaaattctt ccacatgttt gttgattcgt atcctggtgt aggtttacct
      361 tcacttgtac tcacaatgta atcaccagca tatttttcaa tttcttcttg tttcacttca
      421 gcccaacctg cttttgcagt taacttttgt tgttctggtt gcattttcaa accaaatgct
      481 tgatataata cttctccacc acgaccccag ttatcgccgt aagtgtataa ttttttatca
      541 aattcatcaa acaatgacac tgttgcatct tgtccaattg cttttttaat ttctttaccg
      601 tctttagcag ttgtttcttc ccaatctttc ttccaagctt ttactttatc ttctttacca
      661 acaattttcc ctaacatttc ttgttgttct aaatatttat gcttattata gtcaacaact
      721 actgttggtg ctactttttg atatttttta atatctttgt cagtagagta tacaataatt
      781 aaatctggct tttctttagc aactttttct acatcgccat caccaatttt tgtaacacct
      841 ttaaatttat cttttaatac tttgctttga tcgacttgtt gatttacagc tacaatgttt
      901 gcacctaatt ttttaagtcc accagcatat gttggcgcaa ctactgcaat gcgtttaggg
      961 tctttcggaa tatctaccgt tttgccatcg tccattttat aagatttagt ttcagctttg
     1021 ttattttttt caccttggtt cccacacgca gctaacacta ataacataat tattaatggt
     1081 aataatagtt ttttcataat ttcctcctat tgaaaatgat tatcaatcat tcaaaaatta
     1141 tacaagattg catatacatt tacaatagct agtgttattt attttttatt ttcgcaaaaa
     1201 atttaacaaa atatcttaac atccctataa atccccacaa aaaaaagggt aaatccgaac
     1261 gtcaaatttc atctctttat gccaatcaac gatcgattta ccttttaaaa tttaaaatgt
     1321 atctgtaata ctcttagcaa tatttgtata tgctgcatct tccataggtg gattgtgtaa
     1381 tcctgcacgc atgtcacgat agtaacgttg taatggtcga ttcatttcta agcttttagc
     1441 accaactatt ctcatcgcta aatcaatcac ttcaagccct tgattcatta ccatcacttt
     1501 actcgcactt gttggatttc ttatctgact gtcatcttta tattgttgat acccttttgc
     1561 tgtactccat aaaaattgtc tcgcacataa caatagcgtt tccattttcc ctaaattttg
     1621 ttgcacagtt ggcaatgttg caatcgttcc ttcaatacta ttagggctat gttggattgc
     1681 aaagtctaca gcataatttc tagcagcttg tgctatacct aaataacagc ttggtatatg
     1741 caatatccaa ccatttggcg ctttactttt ctctgtttcc actaaatgtt ttaatggtac
     1801 ttttacatca tttaatatta aatcatgact ttctgtcgct cgcataccta atacgtccca
     1861 attatcggca atttcaacac caggcaaatt cctgtctact aagaaaaaac caacactttc
     1921 taattcttct atataagcag caacaataat atgtgttagt gctttactca ttgatgtata
     1981 tgtcttcaca ccatttaaaa tatacccatc atcagcttta acagcatgtg tacttggtct
     2041 tccccctctt gttggactac ccatttcagc ttcactaact gctctattaa ctaatgcacc
     2101 attattaatt tcaacagcaa attgctccaa catatcttga gaccataatt tctgttcata
     2161 aatttgtcct acgacactca catgccaacc aatagataat gctgtggcac catcaagttc
     2221 gcctaaaaat gattgcaaaa taaccatgtc ttctatggtc gcaccttcac caccatatgc
     2281 ttttggtaac gttaattttc cataaccttc tttaattaac cattcaatat ttttatatgg
     2341 aaattggcta tgttgatcat tatattcagc tttagcctta aatgtatctc taattacttt
     2401 gaatttttca atccattttc tttgaatttc cgttgttatt aaaaaggaat tatccatatc
     2461 attcatatta gtcaccccac acaacacaca atataaatta tttattccta gtttaacact
     2521 cgactttaaa aataaaaagg aacgtgctgt gaacaattaa aagaaaactg ataattcgat
     2581 taagtatgca cttaatttta tccataaaaa tacaccttaa actatacaat tcgctgtata
     2641 gtttaaggtg atattcaaac aatattaggt ttgatttaac tatcaagcat ctactttgtc
     2701 atgtttaata ccagcaaata ataaaatcca tgtcacgata ataaatggca atgttaatgc
     2761 aggtaagcca aacggttcaa gcaatgttgt tgtacctagt tgcaccacta ctgttaataa
     2821 cacacctaaa aaagtcgaaa tataagggtt aatcgctgtt ttaaatgtat atcctagtgc
     2881 gattgccatt aatacaaagt tataaccgaa taatccctga ttaatatcat cataattacc
     2941 acctaataga gctacaatga taaagcttaa caaactagct ataacagcta agagtgttgc
     3001 ttttcttgat gctatcaata tgccgattaa aatacatacg ccaccaatta cactcgcttc
     3061 gataaatact tgactgaatc cttcaaataa tgactgaatg aaatgtattc tatcattgtt
     3121 gctaaaatta accgtttcaa tgttttgagg cattaacttt aacgatgtat ctacaaattt
     3181 aacctgtcct gaaagtaaaa ttgtaaacca agtcacaatg acaaaaggca tcgtcagcat
     3241 cggaacttta tatggtctta aaacttctct cactgcagca gcaactggta acgttaataa
     3301 agttgctatc attgttataa caatatctaa tcctgactta tctaaaaaga ttgttaacgc
     3361 aatggcagtt agcactggat taaatccagc taacccatca ttaatctctg cctcactata
     3421 atttataaaa cgcgcaaaag tataggcgat gatgctacct acaatagccg ctaatccaat
     3481 tgtccaatcg gctacaaata atcctattaa gataaataat cctgtccatt tattactaat
     3541 taacacaacc tgagatatat ttttcaaaag cacatctatt atcttcaaat ctttcacctt
     3601 tcctttgaat catta
//
LOCUS       DAXWFK010000193         3538 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.194, whole genome shotgun sequence.
ACCESSION   DAXWFK010000193 DAXWFK010000000
VERSION     DAXWFK010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.194"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(395..3451)
                     /gene="fnbA"
                     /locus_tag="ACGEXS_002395"
     CDS             complement(395..3451)
                     /gene="fnbA"
                     /locus_tag="ACGEXS_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein FnbA"
                     /protein_id="HFF8013379.1"
                     /translation="MKNNLRYGIRKHKLGAASVFLGTMIVVGMGQDKEAAASEQKTTT
                     VEENGNSATDNKTSETQTTATNVNHIEETQSYNATVTEQPSNATQVTTEEAPKAVQAP
                     QTAQPANIETVKEEVVKEEAKPQVKETTQSQDNSGDQRQVDLTPKKATQNQVAETQVE
                     VAQPRTASESKPRVTRSADVAEAKEASNAKVETGTDVTSKVTVEIGSIEGHNNTNKVE
                     PHAGQRAVLKYKLKFENGLHQGDYFDFTLSNNVNTHGVSTARKVPEIKNGSVVMATGE
                     VLEGGKIRYTFTNDIEDKVDVTAELEINLFIDPKTVQTNGNQTITSTLNEEQTSKELD
                     VKYKDGIGNYYANLNGSIETFNKANNRFSHVAFIKPNNGKTTSVTVTGTLMKGSNQNG
                     NQPKVRIFEYLGNNEDIAKSVYANTTDTSKFKEVTSNMSGNLNLQNNGSYSLNIENLD
                     KTYVVHYDGEYLNGTDEVDFRTQMVGHPEQLYKYYYDRGYTLTWDNGLVLYSNKANGN
                     GKNGPIIQNNKFEYKEDTIKETLTGQYDKNLVTTVEEEYDSSTLDIDYHTAIDGGGGY
                     VDGYIETIEETDSSAIDIDYHTAVDSEAGHVGGYTESSEESNPIDFEESTHENSKHHA
                     DVVEYEEDTNPGGGQVTTESNLVEFDEESTKGIVTGAVSDHTTVEDTKEYTTESNLIE
                     LVDELPEEHGQAQGPVEEITENNHHISHSGLGTENGHGNYDVIEEIEENSHVDIKSEL
                     GYEGGQNSGNQSFEEDTEEDKPKYEQGGNIVDIDFDSVPQIHGQNKGNQSFEEDTEKD
                     KPKYEHGGNIIDIDFDSVPHIHGFNKHTEIIEEDTNKDKPSYQFGGHNSVDFEEDTLP
                     KVSGQNEGQQTIEEDTTPPIVPPTPPTPEVPSEPETPTPPTPEVPSEPETPTPPTPEV
                     PSEPETPTPPTPEVSAEPGKPVPPAKEEPKKPSKPVEQGKVVTPVIEINEKVKAVAPT
                     KKPQSKKSELPETGGEESTNKGMLFGGLFSILGLTLLRRNKKNHKA"
     gene            complement(3435..3536)
                     /locus_tag="ACGEXS_002396"
     CDS             complement(3435..3536)
                     /locus_tag="ACGEXS_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013380.1"
                     /translation="MLLKTEQYRLAFIKFKKLMNFAFKGRYYSEKQS"
BASE COUNT          877 a          694 c          535 g         1432 t
ORIGIN      
        1 atcgcctgta caatgcatca ttaacaagtc actgaaacgc ctttcattgt attaataacg
       61 tcactataat ttttatatcg ttcggttttt gtttgatttt aatgattatt tatacaaaaa
      121 cagccgtatt tcaagccgac attttaaatt taactaaatt tgcatctagt taataattgc
      181 atttatcaaa tttgtcttat tgatccaatc taatttgtac tcacaaacta gtttaaaatt
      241 ctaactttat ctctcagttc gttatcaatc atcagacata aaccaatgaa gcaatcagaa
      301 aacactctaa ttttctatta gaaatttgat ttaatataaa aaaacaggct tacttcatat
      361 aatttatgaa ataaacccgt caatttttgt ttaattatgc tttgtgattc tttttatttc
      421 tgcgtaataa tgttaaacct agaatgctga ataatccgcc gaacaacata cctttgtttg
      481 ttgattcttc tccacctgtt tcaggtagtt cagatttctt agattgtggt tttttagttg
      541 gtgccactgc tttaaccttt tcattgattt caataacagg tgttactact ttaccttgtt
      601 ccactggttt agaaggcttt ttaggttctt ctttggcagg tggtactggt ttaccaggtt
      661 cagctgatac ctctggtgtt ggcggtgttg gagtttctgg ctcactcggc acttctggtg
      721 tcggtggtgt tggtgtttcc ggctcacttg gtacttctgg tgttggtggc gttggtgttt
      781 ccggctcact tggtacttct ggtgtcggtg gcgttggtgg cacgattgga ggtgttgtat
      841 cttcttcaat cgtttgttga ccttcatttt ggccgcttac ttttggaagt gtatcttctt
      901 caaagtcaac actattgtgt ccaccgaatt gataacttgg tttatcttta tttgtatctt
      961 cttcaataat ttcagtgtgc ttattgaatc cgtgaatatg tggcacactg tcgaagtcga
     1021 tatcaatgat gttaccgcca tgttcatact taggtttgtc tttttctgta tcttcctcga
     1081 atgactgatt acctttattt tgaccatgaa tttgaggtac actatcaaaa tcgatatcta
     1141 cgatattgcc accttgttca tatttaggtt tgtcttcttc tgtgtcttcc tcgaatgact
     1201 ggttaccgct attttggcca ccttcataac ctaattcact cttaatatca acgtggctat
     1261 tttcttcgat ttcttcaatc acgtcataat tcccgtgacc attttcagtt cctaaaccag
     1321 aatgagaaat atgatgattg ttttcagtaa tttcctcgac tggtccttgt gcttgaccat
     1381 gctcttcagg taattcatcc actaattcaa tcagattact ttcagttgta tattctttcg
     1441 tatcttcaac tgttgtatga tcgctcactg cgccagttac aatacctttt gtagactctt
     1501 cgtcaaattc aactaagtta gactcagtag taacctgacc accacctggg tttgtatctt
     1561 cttcatattc aacaacatca gcgtgatgtt ttgaattttc atgtgtagat tcttcaaagt
     1621 caattggatt tgattcctca gaggactcag tgtatcctcc aacgtgacct gcttcgctat
     1681 ccacagcagt atggtaatcg atatcaatag ctgatgaatc cgtttcttct attgtttcaa
     1741 tgtatccatc aacatatcca cctccaccat ctatagctgt gtggtaatca atgtcaagag
     1801 ttgatgaatc atattcctct tcaacagtag ttactaaatt cttatcatat tgacctgtaa
     1861 gagtttcttt aattgtatct tctttatatt caaatttatt attttgaata atcggaccat
     1921 ttttcccatt tccgttcgct ttattactgt ataaaactaa accattatcc caagttaagg
     1981 tatatcctct atcataataa tacttataaa gttgctctgg atgtcctacc atttgtgttc
     2041 taaaatcaac ttcatcagta ccatttaaat actctccatc atagtgaaca acataagttt
     2101 tatctagatt ttctatattc aatgaatagc ttccattatt ttgtaaattc aaattcccac
     2161 tcatattact tgtgacttct ttaaatttag aagtatctgt cgtatttgca tatacactct
     2221 tcgctatgtc ttcattatta cccaagtatt caaatatcct aacttttggt tgatttccat
     2281 tctgattact acctttcatt aaagttccag taacagtcac acttgtcgtt ttaccattat
     2341 taggtttaat aaatgcaaca tgcgaaaatc tattattcgc tttattaaat gtctcaatcg
     2401 atccatttaa attggcataa taattcccaa taccatcttt atatttaaca tctaattcct
     2461 ttgaagtttg ttcttcattt agtgttgaag ttatagtttg atttccatta gtttgtacag
     2521 ttttaggatc aataaataaa ttaatttcta gttcagccgt tacatcaacc ttatcttcaa
     2581 tatcatttgt aaatgtatat ctaatctttc caccttctaa aacttcacct gtcgccatta
     2641 cgactgaacc atttttaatt tctggtactt ttctagcagt tgatacgcca tgcgtattta
     2701 cattatttga taaagtaaag tcaaagtagt caccttgatg taaaccattc tcaaatttca
     2761 acttatattt tagtaccgct cgttgtcctg catgaggttc tactttattt gtattgttat
     2821 gcccctcaat agaaccaatt tctactgtaa ctttacttgt tacatctgta cccgtttcca
     2881 ctttcgcgtt actagcttcc ttagcttccg ctacatctgc tgatcttgtc acacgtggct
     2941 tactttctga tgccgttctt ggctgtgcca cttcaacttg tgtttctgcg acttgatttt
     3001 gtgtagcctt tttaggtgtt aaatctactt gtctttgatc tccgctattg tcttgagatt
     3061 gtgttgtttc cttaacttga ggtttcgctt cttccttaac tacctcttct ttaactgttt
     3121 ctatatttgc tggttgtgca gtttgtggtg cttgtactgc ttttggtgct tcttcagttg
     3181 ttacttgtgt tgcgtttgac ggttgttctg ttactgttgc gttatatgat tgagtttctt
     3241 ctatatgatt aacgttagtt gcagttgttt gtgtttcact tgttttatta tcagtagctg
     3301 aattcccatt ttcttctact gtagttgtct tttgttctga tgctgcagct tctttgtctt
     3361 gtcccatccc aacaacgatc attgttccta agaatactga tgctgctccc aatttatgtt
     3421 ttctaatgcc gtacctaaga ttgtttttca ctataatatc tccctttaaa tgcaaaattc
     3481 attaattttt taaacttaat aaatgcaagt ctatattgtt cggtttttaa aagcaata
//
LOCUS       DAXWFK010000194         3518 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.195, whole genome shotgun sequence.
ACCESSION   DAXWFK010000194 DAXWFK010000000
VERSION     DAXWFK010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.195"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            66..1463
                     /gene="sufB"
                     /locus_tag="ACGEXS_002397"
     CDS             66..1463
                     /gene="sufB"
                     /locus_tag="ACGEXS_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFF8013381.1"
                     /translation="MAKKAPDVGDYKYGFHDDDVSIFRSERGLTENIVREISNMKNEP
                     EWMLDFRLKSLKLFYKMPMPQWGGDLSELNFDDITYYVKPSEQAERSWDEVPEEIKRT
                     FDKLGIPEAEQKYLAGVSAQYESEVVYHNMEKELEEKGIIFKDTDSALQENEELFKKY
                     FASVVPAADNKFAALNSAVWSGGSFIYVPKNIKLDTPLQAYFRINSENMGQFERTLII
                     ADEGASVHYVEGCTAPVYTTSSLHSAVVEIIVHKDAHVRYTTIQNWANNVYNLVTKRT
                     FVYENGNMEWVDGNLGSKLTMKYPNCVLLGEGAKGSTLSIAFAGKGQVQDAGAKMIHK
                     APNTSSTIVSKSISKNGGKVIYRGIVHFGRKAKGARSNIECDTLILDNESTSDTIPYN
                     EVFNDQISLEHEAKVSKVSEEQLFYLMSRGISEEEATEMIVMGFIEPFTKELPMEYAV
                     EMNRLIKFEMEGSIG"
     gene            complement(1531..2580)
                     /locus_tag="ACGEXS_002398"
     CDS             complement(1531..2580)
                     /locus_tag="ACGEXS_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8013382.1"
                     /translation="MPVYKNDNTGKWYFSIRYKDVYGNNKRKMQRGFSTKREAKRAEA
                     IFLNDVNEGYSDSKTFDYVFHHYLENSDLRPKTKRRKQNEYHKHFKAKFGHIKMNKIT
                     QNQCQEFRKYLIENVASTNSARTIWSGFKVVINYAKKYFGLRTDPTISIKPIPRVKPK
                     PKFMMREEFEERIKDIEEQDYRELFTLMFYTGLRIGEAMALVWTDYNKYKKEISINKT
                     MDISNRTIYPRPKTDSSEDIVPLPKFINTMLTERHQREKELNKYFDERCYFIFGGMAP
                     KHYSHVQKKFQKAFPHYNIHALRHSYASYLANNGVDIFVLQSLMRHAQITETMGTYSH
                     LYTQKKHDAIAIFDK"
     gene            2693..2872
                     /locus_tag="ACGEXS_002399"
     CDS             2693..2872
                     /locus_tag="ACGEXS_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFF8013383.1"
                     /translation="MCVKFTDAEIAYIKESVENYSSEFDIYDDEQELKLKIYEQIMLK
                     IESEYKDIYLFRLIN"
     gene            complement(2852..>3518)
                     /locus_tag="ACGEXS_002400"
     CDS             complement(2852..>3518)
                     /locus_tag="ACGEXS_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013384.1"
                     /translation="TSYIYDSKKYVEEQSGLLDFNNYIYYTSIYYGKYIGTVIIQNNL
                     DLFFNYGKRLANDHYNTLISKSKEKLINKAHDEIQPFNHLDLNSMKKIVDDITFSYQI
                     EQGLQAYKRELYLPAAATFAVAIETFLIKLKKVNKIKHKDTDSTMYTKLLGELTKEGK
                     VNYRTKKRVEIAYSMRNIINHSQAGAVAKGDCDFLLNTLKDIVDENERILTEYSKSIN
                     KTE"
BASE COUNT         1136 a          537 c          600 g         1245 t
ORIGIN      
        1 ggtttacatc atgaattaaa aacttacgca cgccgttgta aatatatttt taaggagtga
       61 ttgaaatggc taaaaaagca cctgatgttg gggattataa atatggattc cacgacgatg
      121 atgtgtccat tttcagatca gaacgtggtt taactgagaa tatcgttaga gaaatttcta
      181 acatgaaaaa tgagccggaa tggatgttag atttccgtct taaatcatta aaattgtttt
      241 ataaaatgcc aatgcctcaa tggggtggcg acttatcaga attgaatttc gatgacatta
      301 cttactatgt aaagccttca gaacaagctg aacgttcatg ggatgaagtg ccagaagaaa
      361 ttaaaagaac tttcgataaa ttaggaattc ctgaagctga acaaaaatat ttagctggtg
      421 tttctgctca atatgaatct gaagttgttt accataatat ggaaaaagaa cttgaagaaa
      481 aaggtattat ctttaaagat acagatagtg ctttacaaga aaatgaagaa ttattcaaaa
      541 aatactttgc ttctgtagta cctgcagcag ataacaaatt tgcggcgtta aactcagcag
      601 tatggtcagg tggttcgttc atttatgtac ctaaaaatat caaactagat acgccactac
      661 aagcttattt ccgtattaac tctgagaaca tgggtcaatt tgaacgtaca ttaatcattg
      721 ctgatgaagg tgcttctgta cattacgtag aaggttgtac tgcaccagtt tatacaacta
      781 gttctttaca ctctgctgtt gtggaaatca ttgtgcataa agatgcgcac gttcgttata
      841 ctacgattca aaactgggcg aacaatgtat acaatttagt tacaaaacgt acttttgttt
      901 atgaaaacgg aaatatggaa tgggtagatg gtaacttagg ttctaagtta acgatgaaat
      961 atccaaactg tgttctttta ggtgaaggtg caaaaggtag tacattatct attgcatttg
     1021 ctggtaaagg acaagttcaa gatgccggtg ctaaaatgat tcataaagca cctaatacat
     1081 cttctacaat tgtttctaaa tctatttcta aaaatggtgg taaagttatt tatcgcggta
     1141 ttgttcattt tggacgtaaa gcaaaaggtg ctcgttcaaa tattgaatgt gatacattaa
     1201 tcttagataa cgaatcaaca tcagatacaa ttccatataa cgaagtattc aacgatcaaa
     1261 tatcattaga acatgaagcc aaggtttcaa aagtttctga agaacaatta ttctatctaa
     1321 tgagtcgtgg tatttctgaa gaagaagcga cagaaatgat tgttatggga ttcatcgaac
     1381 catttacaaa agaacttcca atggaatacg cggtcgaaat gaaccgttta atcaagttcg
     1441 aaatggaagg tagtattgga tagctttaaa ccgcgttgtt aagccattct taacttccgg
     1501 aaatggctat tgataccatt ttgataccat ttacttgtca aaaatggcta ttgcatcgtg
     1561 ttttttctga gtatataaat ggctgtaagt gcccatcgtt tcagtgattt gagcatgtct
     1621 catgagtgac tgtaaaacga aaatatctac accattattt gcaagataag atgcataaga
     1681 atgtcttaac gcgtgaatgt tataatgggg gaaagctttt tgaaatttct tttgaacatg
     1741 actgtaatgt ttgggagcca ttcctccgaa aataaaataa caacgttcat caaaatattt
     1801 gtttaactct ttttcacgtt gatgtcgttc agttaacatt gtattgatga atttaggtaa
     1861 aggaacaata tcctctgaac tatctgtttt tggtctcgga tatatagttc tattagagat
     1921 gtccattgtt ttatttatgg atatctcttt tttgtattta ttgtagtctg tccaaacaag
     1981 agccatagct tcgccaatcc ttaaacctgt ataaaacatt aatgtaaata actctctgta
     2041 atcttgctct tcaatgtctt tgattctttc ttcaaattct tcacgcatca taaacttagg
     2101 ttttggcttt acacgcggaa taggtttaat tgatattgtt ggatctgtac gtaatccaaa
     2161 gtatttttta gcataattaa ttacaacttt aaaacctgac caaattgtac gagcagaatt
     2221 tgttgatgct acattctcta ttagatattt acgaaactct tggcattgat tttgtgttat
     2281 cttattcatt tttatgtgcc cgaacttagc tttaaagtgt ttatgatatt cattttgttt
     2341 gcgtcgtttt gttttaggtc tcaaatcgct attttctaaa tagtgatgaa aaacataatc
     2401 aaatgttttc gaatcactat atccttcgtt tacgtcattc aaaaaaatag cctctgctct
     2461 cttagcttca cgcttagttg aaaaaccgcg ttgcatctta cgtttgttat taccgtatac
     2521 atctttatat ctaatggaaa aataccattt acctgtatta tcattcttat atactggcat
     2581 tttgcttctc cctcctcaaa attggcaaaa aaataataag ggtaggcgag ctacccgaaa
     2641 tttagtacta ggtactaaat gtgatataat aaaataaaaa gtaggtgatg aaatgtgcgt
     2701 aaagtttact gacgcagaaa tagcttatat aaaagaatca gttgaaaatt atagtagtga
     2761 atttgatatt tatgacgatg aacaagaact taaattaaaa atttatgaac aaattatgtt
     2821 aaaaatagag tccgaataca aggatatcta tttattccgt cttattaatt gatttactgt
     2881 attcggttaa tattctttcg ttttcatcaa cgatgtcctt tagtgtgttt aaaaggaagt
     2941 cgcaatcacc tttggctact gcaccggctt gtgaatggtt gattatattt ctcatactat
     3001 aagcaatttc tacccgtttt ttggttctat aattcacttt accctcttta gttaattctc
     3061 ctaataattt ggtgtacata gttgaatcgg tgtctttatg tttgatttta ttcacttttt
     3121 ttaatttgat taaaaacgtc tctatagcaa cagcaaaggt tgctgcagct ggcaaataca
     3181 actccctttt ataagcttgt aatccttgtt ctatttgata agaaaaagtt atatcatcaa
     3241 caatcttttt catgctattt aaatctaagt ggttgaacgg ttgtatttca tcatgtgctt
     3301 tgtttatcaa tttctctttc gacttcgata tcaatgtatt gtaatgatcg ttagctaatc
     3361 gtttgccata attaaaaaat aaatctaaat tgttttgtat tattacagtc ccgatatatt
     3421 ttccgtagta aatagacgtg taataaatgt agttattaaa atctaataat ccggattgtt
     3481 cttctacata ctttttagaa tcatatatgt atgaagta
//
LOCUS       DAXWFK010000195         3499 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.196, whole genome shotgun sequence.
ACCESSION   DAXWFK010000195 DAXWFK010000000
VERSION     DAXWFK010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.196"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            164..1393
                     /locus_tag="ACGEXS_002401"
     CDS             164..1393
                     /locus_tag="ACGEXS_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HFF8013385.1"
                     /translation="MSLRDEALEMHKRNQGKLEVKPNVKVTNKEELSLAYSPGVAEPC
                     KDIYEDKRKVYDYTIKGNTVAVITDGTAVLGLGNIGPEASIPVMEGKAVLFKSFAGIN
                     GVPIALNTTDTEEIIKTVKLLEPNYGGINLEDISAPRCFEIEERLKKETNIPVFHDDQ
                     HGTAIVTLAGLVNALRVVNKDIAKIKVVLNGAGAAGIAIVKLLYAYGVRNMVMCDSRG
                     AIFEGRSYGMNPTKDVVAKWTNKDKIEGSLEEVVKDADVFIGVSVANALSQDMVKSMA
                     DNPIIFAMANPNPEIIPDDAKAAGARVVGTGRSDYPNQINNVLAFPGIFRGALEVEAT
                     HINEEMKKAAVEAIADLIDSSELNEDYCIPGPFDKRVAPSVARNVAKAAMESGVARIE
                     VDPQDVYDKTMKLTDLQ"
     gene            1588..2445
                     /gene="accD"
                     /locus_tag="ACGEXS_002402"
     CDS             1588..2445
                     /gene="accD"
                     /locus_tag="ACGEXS_002402"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFF8013386.1"
                     /translation="MFKDFFNRTKKKKYLTVQDSKNNDVPAGIMTKCPKCKKIMYTKE
                     LAENLNVCFNCDHHIALTAYKRIEAISDEGSFTEFDKGMTSANPLDFPSYLEKIEKDQ
                     QKTGLKEAVVTGTAQLDGMKFGVAVMDSRFRMGSMGSVIGEKICRIIDYCTENRLPFI
                     LFSASGGARMQEGIISLMQMGKTSVSLKRHSDAGLLYISYLTHPTTGGVSASFASVGD
                     INLSEPKALIGFAGRRVIEQTINEKLPDDFQTAEFLLEHGQLDKVVHRNDMRQTLSEI
                     LKIHQEVTK"
     gene            2445..3389
                     /locus_tag="ACGEXS_002403"
     CDS             2445..3389
                     /locus_tag="ACGEXS_002403"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HFF8013387.1"
                     /translation="MLDFEKPLFEIRNKIESLKESQDKNDVDLQEEIDMLEASLERET
                     KKIYTNLKPWDRVQIARLQERPTTLDYIPYIFDSFMELHGDRNFRDDPAMIGGIGFLN
                     GRAVTVIGQQRGKDTKDNIYRNFGMAHPEGYRKALRLMKQAEKFNRPIFTFIDTKGAY
                     PGKAAEERGQSESIATNLIEMASLKVPVIAIVIGEGGSGGALGIGIANKVLMLENSTY
                     SVISPEGAAALLWKDSNLAKIAAETMKITAHDIKQLGIIDDVISEPLGGAHKDIEQQA
                     LAIKSAFVAQLDSLESLSRDEIANDRFEKFRNIGSYIE"
BASE COUNT         1242 a          471 c          698 g         1088 t
ORIGIN      
        1 tttataaata ttattttatt atcgtttatc gctatttgtg ttacataatc aaaaccataa
       61 attcttactc attcagattt acccaatatt tttactttta taatgtaatg cgttttatcc
      121 aagttatttt ttaaaaataa atattgaatt aggggctgat ttcatgtcat taagagatga
      181 agcattggaa atgcacaaac gtaatcaagg taaattagaa gttaaaccaa atgtaaaagt
      241 tactaataaa gaggaattaa gtttagcata ctcacctggc gttgctgaac cgtgtaaaga
      301 tatttatgaa gataaaagaa aagtatatga ttacacaatt aaaggaaata cagttgcagt
      361 tattactgat ggaacagcgg tattaggttt aggtaacatt ggacctgaag caagtattcc
      421 tgtaatggaa ggtaaagcag tattattcaa aagcttcgct ggtatcaatg gggtgcctat
      481 tgcgttaaat acaactgata ccgaagaaat cattaaaaca gttaagttgt tagaacctaa
      541 ttatggtggt attaatttag aggatatttc ggcaccacgt tgttttgaaa ttgaagaacg
      601 attgaaaaaa gaaactaata ttccggtatt ccatgacgat caacatggta cagcaattgt
      661 aacattggca ggtttggtaa atgcattgag agttgttaac aaagatattg ctaaaataaa
      721 agttgtacta aatggtgctg gtgcagcagg aatagccatt gttaaattac tatacgcgta
      781 tggtgtaaga aatatggtta tgtgtgactc aagaggcgca atttttgaag gacgttcata
      841 tggtatgaat cctacgaaag atgttgtagc aaaatggaca aataaagata agattgaagg
      901 gtctttagaa gaagtcgtaa aagacgcaga tgtatttatc ggggtttctg tagctaatgc
      961 gctgtcacaa gatatggtta agagtatggc agataatcca attatatttg caatggctaa
     1021 tccaaatcct gaaataatac ctgatgatgc caaagcggca ggtgcacgag ttgttggtac
     1081 aggacgttca gactatccta accaaattaa taatgtatta gctttccctg gtatttttag
     1141 aggtgcatta gaggttgaag ctacacatat aaatgaagaa atgaaaaagg cagctgtaga
     1201 agcgattgct gatttaatcg atagttctga attaaatgaa gactactgta tcccaggacc
     1261 gtttgataaa cgtgtagcgc catcagttgc tcgtaatgtt gctaaagcgg caatggaatc
     1321 tggagtagct aggattgaag ttgatccgca agatgtgtat gataaaacaa tgaaacttac
     1381 agatttacaa taaaaataat taacgtttaa ttaaattgct taaaagttag caataaagag
     1441 tcattaaaat gatgaaaatg aaagtttatg ataaacattc aacagtcaaa cgaatataaa
     1501 tcaaataaat ttaaacccgt ttttaactgg tcaagttcag tttaaggctc taaatggtta
     1561 gaacagaggt tatttggagg ttttcctatg tttaaagatt tttttaatcg aacaaagaaa
     1621 aagaaatatc ttacagtaca agactctaaa aataatgatg tgcctgcagg tattatgact
     1681 aagtgtccaa agtgtaagaa aattatgtac acaaaagaat tagctgaaaa tttaaatgtg
     1741 tgctttaatt gtgatcatca tattgcttta actgcgtata aacgtataga agcaatttct
     1801 gatgaaggat catttacaga attcgataag ggaatgacct ctgcgaatcc attagatttt
     1861 ccaagttatt tagaaaaaat tgaaaaggac caacaaaaga caggtcttaa agaagcagtt
     1921 gtgactggta cagcacaact agatggtatg aaatttggcg ttgctgtcat ggattcacgt
     1981 tttagaatgg gaagtatggg atcggttatc ggtgaaaaga tatgtcgcat cattgattac
     2041 tgcactgaga accgtttacc atttattctt ttctctgcaa gtggtggtgc acgtatgcaa
     2101 gaaggtatta tttccttgat gcaaatgggt aaaaccagtg tatctttaaa acgtcattct
     2161 gacgctggac tattatatat atcatattta acacatccaa ctactggtgg tgtatctgca
     2221 agttttgcat cagttggtga tataaattta agtgagccaa aagcgttgat aggttttgca
     2281 ggtcgtcgag ttattgaaca gacaataaac gaaaaattgc cagatgattt ccaaactgca
     2341 gaatttttat tagagcatgg acaattggat aaagttgtac atcgtaatga tatgcgtcaa
     2401 acattgtctg aaattctaaa aatccatcaa gaggtgacta aataatgtta gattttgaaa
     2461 aaccactttt tgaaattcga aataaaattg aatctttaaa agaatctcaa gataaaaatg
     2521 atgtggattt acaagaagaa attgacatgc ttgaagcgtc attggaacga gaaactaaaa
     2581 aaatatatac aaatctaaaa ccatgggatc gtgtgcaaat tgcgcgtttg caagaaagac
     2641 ctacgaccct agattatatt ccatatatct ttgattcgtt tatggaacta catggtgatc
     2701 gtaattttag agatgatcca gcaatgattg gtggtattgg ctttttaaat ggtcgtgctg
     2761 ttacagttat tggacaacaa cgtggaaaag atacaaaaga taatatttat cgaaattttg
     2821 gtatggcgca tccagaaggt tatcgaaaag cattacgttt aatgaaacaa gctgaaaaat
     2881 tcaatcgtcc tatctttaca tttatagata caaaaggtgc atatcctggt aaagctgctg
     2941 aagaacgtgg acaaagtgaa tctatcgcaa caaatttgat tgagatggct tcattaaaag
     3001 taccagttat tgcgattgtc attggtgaag gtggcagtgg aggtgctcta ggtattggta
     3061 ttgccaataa agtattgatg ttagagaata gtacttactc tgttatatct cctgaaggtg
     3121 cagcggcatt attatggaaa gacagtaatt tggctaaaat tgcagctgaa acaatgaaaa
     3181 ttactgccca tgatattaag caattaggta ttatagatga tgtcatttct gaaccacttg
     3241 gcggtgcaca taaagatatt gaacagcaag ctttagctat taaatcagcg tttgttgcac
     3301 agttagattc acttgagtca ttatcacgtg atgaaattgc taatgatcgc tttgaaaaat
     3361 tcagaaatat cggttcttat atagaataat caacttgagc atttttatgt taaatcgata
     3421 ctgggtttta ccataaattg aagtacatta aaacaataat ttaatattta gatactgaat
     3481 tttaactaag attagtagt
//
LOCUS       DAXWFK010000196         3493 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.197, whole genome shotgun sequence.
ACCESSION   DAXWFK010000196 DAXWFK010000000
VERSION     DAXWFK010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.197"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            73..1542
                     /locus_tag="ACGEXS_002404"
     CDS             73..1542
                     /locus_tag="ACGEXS_002404"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFF8013388.1"
                     /translation="MYQLEDDSLMLHNDLYQINMAESYWNDNIHEKMAVFDLYFRKMP
                     FNSGYAVFNGLKRVIDFIEHFGFSESDLEYLKSIGYKDDFLSYLKDLKFTGSIRSMQE
                     GELCFGNEPLLRVEAPLIQAQLIETILLNIVNFHTLITTKASRIRQIASNDKLMEFGT
                     RRAQEIDAALWGARAAYIGGFDSTSNVRAGKLFGIPVSGTHAHAFVQTYGDEYVAFKK
                     YAERHKNCVFLVDTFHTLKSGVPNAIKVAKELGDKINFVGIRLDSGDIAYLSKEARRM
                     LDEAGFTETKIIASNDLDEETITSLKAQGAKVDSWGVGTKLITGYDQPALGAVYKLVA
                     IENENGSYSDRIKLSNNAEKVTTPGKKNVYRIINKKTGKAEGDYITLENENPYDEQPL
                     KLFHPVHTYKMKFIKSFEAIDLHHNIYENGKLVYQMPTEDESREYLALGLQSIWDENK
                     RFLNPQEYPVDLSKACWDNKHKRIFEVAEHVKEMEEDNE"
     gene            1535..2356
                     /gene="nadE"
                     /locus_tag="ACGEXS_002405"
     CDS             1535..2356
                     /gene="nadE"
                     /locus_tag="ACGEXS_002405"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFF8013389.1"
                     /translation="MSKLQDVIVQEMKVKKRIDSAEEIMELKQFIKNYVQSHSFIKSL
                     VLGISGGQDSTLVGKLVQMSVNELREEGIDCTFIAVKLPYGVQKDADEVEQALRFIEP
                     DEIVTVNIKPAVDQSVQSLKEAGIVLTDFQKGNEKARERMKVQFSIASNRQGIVVGTD
                     HSAENITGFYTKYGDGAADIAPIFGLNKRQGRQLLAYLGAPKELYEKTPTADLEDDKP
                     QLPDEDALGVTYEAIDNYLEGKPVTPEEQKVIENHYIRNAHKRELAYTRYTWPKS"
     gene            2627..3229
                     /locus_tag="ACGEXS_002406"
     CDS             2627..3229
                     /locus_tag="ACGEXS_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFF8013390.1"
                     /translation="MSFVTENPWLMVLTIFIINVCYVTFLTMRTILTLKGYRYIAASV
                     SFLEVLVYIVGLGLVMSNLDHIQNIIAYAFGFSIGIIVGMKIEEKLALGYTVVNVTSA
                     EYELDLPNELRNLGYGVTHYAAFGRDGSRMVMQILTPRKYERKLMDTIKNLDPKAFII
                     AYEPRNIHGGFWTKGIRRRKLKDYEPEELESVVEHEIQSK"
     gene            3210..3383
                     /locus_tag="ACGEXS_002407"
     CDS             3210..3383
                     /locus_tag="ACGEXS_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NETI motif-containing protein"
                     /protein_id="HFF8013391.1"
                     /translation="MKFKVNENETIADCLSRMKMQGYMPVKRIEKPVFQEQKDGTVEV
                     SHQEIVFVGKKIQ"
BASE COUNT         1272 a          453 c          688 g         1080 t
ORIGIN      
        1 gctcttatgt atataaaaaa atgaattatg ataaaatgta agaaaatatt taggtcgatt
       61 ggagagatac aagtgtacca attagaagac gacagtttaa tgttacataa tgacttatat
      121 caaataaata tggctgaaag ttattggaat gataatattc atgaaaaaat ggctgtattt
      181 gatttgtatt ttagaaaaat gccatttaat agtggctatg ctgtttttaa tggtttaaaa
      241 cgcgtcattg attttataga acattttgga ttttcagaat ctgatttgga atatttaaag
      301 tctattggct acaaggatga tttcttatca tatttaaaag atttaaaatt cacaggcagc
      361 atccgttcga tgcaagaagg cgaattatgc tttggtaacg aaccattgtt acgcgtagaa
      421 gcaccattga ttcaagcgca attaatagaa acaattttat taaacattgt aaatttccat
      481 acattaatta caacaaaggc tagcagaatt cgtcaaattg catcaaatga taaattaatg
      541 gagtttggta cacgtcgtgc gcaagaaatt gatgcagcat tgtggggcgc tagagctgct
      601 tacatcgggg gctttgattc tacaagtaat gttagggcgg ggaaattatt tggtatacct
      661 gtgtctggta cacatgcaca tgcatttgtc caaacttatg gagacgaata tgttgccttc
      721 aaaaaatatg ctgaaagaca taaaaattgt gtgttcctag tagatacatt ccatacttta
      781 aaatctggcg tgccaaatgc aataaaagtt gcaaaagaat taggtgacaa aattaacttt
      841 gtaggtattc gattagattc tggagatatc gcttatttat ctaaagaggc aagacgtatg
      901 cttgatgaag caggatttac tgaaactaaa attatcgcgt ctaatgattt ggatgaagaa
      961 acgattacga gtttgaaagc acaaggtgca aaagtagatt cttggggcgt tggtacaaag
     1021 ctgattacag gatacgatca accagcatta ggtgcagtat ataaacttgt agctattgaa
     1081 aatgaaaatg gttcatatag tgatcgtatt aaattatcaa ataacgctga aaaggttacg
     1141 acgccaggta agaaaaatgt atatcgcatt ataaacaaga aaacaggtaa ggcagaaggc
     1201 gattatatta ctttggaaaa tgaaaatcca tacgatgaac aacctttaaa attattccat
     1261 ccagtgcata cttataaaat gaaatttata aaatctttcg aagccattga tttgcatcat
     1321 aatatttatg aaaatggtaa attagtatat caaatgccaa cagaagatga atcacgtgaa
     1381 tatttagcac taggattaca atctatttgg gatgaaaata agcgtttcct gaatccacaa
     1441 gaatatccag tcgatttaag caaggcatgt tgggataata aacataaacg tatttttgaa
     1501 gttgcggaac acgttaagga gatggaagaa gataatgagt aaattacaag acgttattgt
     1561 acaagaaatg aaagtgaaaa agcgtatcga tagtgctgaa gaaattatgg aattaaagca
     1621 atttataaaa aattatgtac aatcacattc atttataaaa tctttagtgt taggtatttc
     1681 aggaggacag gattctacat tagttggaaa actagtacaa atgtctgtta acgaattacg
     1741 tgaagaaggc attgattgta cgtttattgc agttaaatta ccttatggag ttcaaaaaga
     1801 tgctgatgaa gttgagcaag ctttgcgatt cattgaacca gatgaaatag taacagtcaa
     1861 tattaagcct gcagttgatc aaagtgtgca atcattaaaa gaagccggta ttgttcttac
     1921 agatttccaa aaaggaaatg aaaaagcgcg tgaacgtatg aaagtacaat tttcaattgc
     1981 ttcaaaccga caaggtattg tagtaggaac agatcattca gctgaaaata taactgggtt
     2041 ttatacgaag tacggtgatg gtgctgcaga tatcgcacct atatttggtt tgaataaacg
     2101 acaaggtcgt caattattag cgtatcttgg tgcgccaaag gaattatatg aaaaaacgcc
     2161 aactgctgat ttagaagatg ataaaccaca gcttccagat gaagatgcat taggtgtaac
     2221 ttatgaggcg attgataatt atttagaagg taagccagtt acgccagaag aacaaaaagt
     2281 aattgaaaat cattatatac gaaatgcaca caaacgtgaa cttgcatata caagatacac
     2341 gtggccaaaa tcctaattta attttttctt ctaacgtgtg acttaaatta aatatgagtt
     2401 agaattaata acattaaacc acattcagct agactacttc agtgtataaa ttgaaagtgt
     2461 atgaactaaa gtaagtatgt tcatttgaga ataaattttt atttatgaca aattcgctat
     2521 ttatttatga gagttttcgt actatattat attaatatgc attcattaag gttaggttga
     2581 agcagtttgg tatttaaagt gtaattgaaa gagagtgggg cgccttatgt cattcgtaac
     2641 agaaaatcca tggttaatgg tactaactat atttatcatt aacgtttgtt atgtaacgtt
     2701 tttaacgatg cgaacaattt taacgttgaa aggttatcgt tatattgctg catcagttag
     2761 ttttttagaa gtattagttt atatcgttgg tttaggtttg gttatgtcta atttagacca
     2821 tattcaaaat attattgcct acgcatttgg tttttcaata ggtatcattg ttggtatgaa
     2881 aatagaagaa aaactggcat taggttatac agttgtaaat gtaacttcag cagaatatga
     2941 gttagattta ccgaatgaac ttcgaaattt aggatatggc gttacgcact atgctgcgtt
     3001 tggtagagat ggtagtcgta tggtgatgca aattttaaca ccaagaaaat atgaacgtaa
     3061 attgatggat acgataaaaa atttagatcc gaaagcattt atcattgcgt atgaacctcg
     3121 aaacatacat ggtggattct ggactaaagg cattcgtcgt agaaagctta aagattatga
     3181 accagaagaa ctggaaagtg tagtagaaca tgaaattcaa agtaaatgag aatgaaacaa
     3241 ttgctgattg tttgtcacga atgaaaatgc aagggtatat gccggtaaaa cgtattgaaa
     3301 aacccgtgtt tcaagagcaa aaagatggca cggttgaagt atcacatcaa gaaatcgttt
     3361 ttgtaggtaa gaaaatccaa taacataatc caatttaaat aaagactatt tgaagaggaa
     3421 aggctattca aagtttgagt aattttactt tgaatagcct atttgtttat acatgcaaga
     3481 tgctcgatcc ata
//
LOCUS       DAXWFK010000197         3485 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.198, whole genome shotgun sequence.
ACCESSION   DAXWFK010000197 DAXWFK010000000
VERSION     DAXWFK010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.198"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            285..638
                     /locus_tag="ACGEXS_002408"
     CDS             285..638
                     /locus_tag="ACGEXS_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HFF8013392.1"
                     /translation="MFSIGSAILHFVIGGIAVALASIIADKVGGKLGGIIATMPAVFL
                     AAIIALALDHRGTQLVEMSMNLSTGAIVGILSCILTVFLTSLYIKHKGYRKGAIFTVV
                     CWFVISLAIFSIRHL"
     gene            706..1101
                     /locus_tag="ACGEXS_002409"
     CDS             706..1101
                     /locus_tag="ACGEXS_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HFF8013393.1"
                     /translation="MKLTLMKFFVGGFAVLLSYIVSVTLPWKEFGGIFATFPAVFLVS
                     MFITGMQYGDKVAVHVSRGAVFGMTGVLVCILVTWMMLHMTHMWLISIVVGFLSWFIS
                     AVCIFEAVEFIAQKRLEKHSWKAGKSNSK"
     gene            1361..1930
                     /locus_tag="ACGEXS_002410"
     CDS             1361..1930
                     /locus_tag="ACGEXS_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8013394.1"
                     /translation="MNLSKQIKKYRERDGYSQEYLAEKLYVSRQSISNWENDKSLPDI
                     HNLLMMCELFNVTLDDLVKGTIPFVPDIKAQRSLNLWTYVMLIFMTLAAILMGPLVVY
                     WNWTWGVTVAIILGIGFYASMKIEDLKKVHKMDNYDRIVAFMNGKDPSEVQTTKARNT
                     MTNALSIISVIGILSLIIFLSVYLANKFL"
     gene            complement(2057..2257)
                     /locus_tag="ACGEXS_002411"
     CDS             complement(2057..2257)
                     /locus_tag="ACGEXS_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFF8013395.1"
                     /translation="MNNGTVKWFNAEKGFGFIEQENGGDVFVHFSGIASDGYKTLEEG
                     QKVTFEITEGQRGDQAVNVQTV"
     gene            complement(2650..2841)
                     /gene="vraH"
                     /locus_tag="ACGEXS_002412"
     CDS             complement(2650..2841)
                     /gene="vraH"
                     /locus_tag="ACGEXS_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter activity
                     modulator VraH"
                     /protein_id="HFF8013396.1"
                     /translation="MKFRELVKQSYEDLKNLTVTWYNVIALTILVIVLSSFTTPMIGI
                     PAGLLGGSYYLKRREEKGK"
     gene            complement(2870..3196)
                     /locus_tag="ACGEXS_002413"
                     /pseudo
     CDS             complement(2870..3196)
                     /locus_tag="ACGEXS_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            3211..3297
                     /locus_tag="ACGEXS_002414"
                     /pseudo
     CDS             3211..3297
                     /locus_tag="ACGEXS_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_039645025.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin C-terminal domain-containing protein"
     gene            3299..>3485
                     /locus_tag="ACGEXS_002415"
     CDS             3299..>3485
                     /locus_tag="ACGEXS_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFF8013397.1"
                     /translation="MIINLETQQIHFDLEHEIPYFQAPEKNRIRLDIDVLNKKQISNI
                     INVIFNNQSKTKCTFLSE"
BASE COUNT         1143 a          495 c          637 g         1210 t
ORIGIN      
        1 agttgactga acgaaaatgc gcttgtaaca agcttttttc aattctagtc aaccttgctg
       61 gggtgggacg acgaaataaa ttttgcgaaa atatcatttc tgtcccactc cctaatttga
      121 gctggatata ctttcatttg aaccctttat tgctagttta tgaaagtatc atgaaagctt
      181 tatgaacatc gcttgagttg cctttacagt agaaaattta agttttacac tttgtgtgaa
      241 tgatacgttt tgtattgaat taattataga aaggtacgtt gaagatgttt tcaattggaa
      301 gtgcaattct tcattttgtc attggtggta tcgctgttgc attagcttca attattgctg
      361 ataaggtagg tggtaagtta ggaggtatta tagctactat gccggcagtc tttcttgcgg
      421 ctattatcgc attagcttta gatcatcgtg gtacgcaatt agtggagatg tcgatgaatc
      481 ttagtactgg agcaattgtc ggtattctgt cttgtatatt aactgtattt ttgacatctc
      541 tctacattaa gcataaaggt tatcggaaag gcgcaatatt cacagttgtt tgttggtttg
      601 tcatttccct cgcaatattc agtattagac atttatagtt tggaaaatgc gtgataatta
      661 gttgtattca gttattaagt aataaattat tggaggcaga acatcatgaa attaacatta
      721 atgaaatttt ttgtgggggg atttgcagta ttattaagtt atattgtatc tgtaacacta
      781 ccttggaaag aatttggcgg tatatttgca acgtttccgg cagtattttt agtgtctatg
      841 tttattacag gtatgcaata tggtgataaa gtcgctgtgc atgtaagtcg tggcgcagtg
      901 tttggtatga caggggtatt agtttgtatt ttagttacat ggatgatgtt acatatgacg
      961 cacatgtggt tgattagcat tgttgttggt ttcctaagct ggttcatcag tgcagtatgt
     1021 atttttgaag cggtagaatt tatagcacaa aaaagattag aaaagcatag ttggaaagct
     1081 ggaaaatcga atagtaaata gtgtgaacgt aatctcttaa ctaggactaa ctttgcaagc
     1141 attgaatagc atggaaaagt tgcatcatta ataagtgaaa ttcaagttgg cattgagaaa
     1201 attacaagcg cgtaatcata caggtctgtc ttaagggagt cttcgaaccc cgatgttgtc
     1261 gtatgtcaaa acatttagtc aatcataaag gtgacttgat ttaactttat ctgatagtct
     1321 gattgtaatg attgtactaa ttgactggag gcgtatgtaa ttgaatctga gtaaacaaat
     1381 taaaaagtat agggaacgag atggttattc acaagaatat cttgctgaaa agttatatgt
     1441 atctaggcag agtatttcta attgggaaaa tgacaaaagc ttaccagaca tacataactt
     1501 attaatgatg tgtgaattgt tcaatgtaac tttagatgat ttagtaaaag ggaccattcc
     1561 atttgtacct gatattaaag cgcaacgaag tcttaactta tggacatatg tgatgcttat
     1621 tttcatgaca ttagctgcaa ttttaatggg acctttagtt gtttattgga attggacttg
     1681 gggtgtaacg gtggcaatca ttttgggaat aggtttttat gcatctatga aaatagaaga
     1741 tttaaaaaaa gtgcataaaa tggacaacta cgatcgaatt gttgctttta tgaatggaaa
     1801 agatcctagt gaagtacaaa cgactaaagc tagaaatacg atgacaaatg cgctttctat
     1861 tatatcagta attggtatac tcagcctcat aattttcctt agtgtgtatt tggcaaataa
     1921 gtttttataa atcatcgtgg tatcgtctca tattatttat attatccaaa atagcataaa
     1981 aaaataccaa caagatttag aaccttgttg gtaatcaaag cgattcattt ataatgagtc
     2041 gttttatgtt gtaagattaa acagtttgta cgttaactgc ttggtctcca cgttgacctt
     2101 cagtgatttc gaaagtaact ttttgacctt cttctaaagt tttgtagcca tcgctagcga
     2161 tacctgagaa atgtacgaat acgtctccgc cattttcttg ttcgatgaaa ccaaaacctt
     2221 tttctgcgtt aaaccatttt actgtaccgt tattcatata gaaaacctcc gtgtgctttt
     2281 gcacttaata tttgtaacaa attcataact aaaaaagagg atattctaaa caaatacact
     2341 acaatttaat tcacgagctt ttattacgta agaccaacta tacgctcata ttggcataat
     2401 gtacagtgtt ttttgaaaat aaattaaaaa agatttttaa aaaccttaga aacgttgatt
     2461 taaaggggtt tataaaaata aaaaaattgt agtcttttat ggtgtttgct agttttcaaa
     2521 gtgacatatc gtttaaacat gatgatttta taagcaatcc ataaaaaaca agcagcgata
     2581 aacgctactt gttgatatta aaatctgact tgaaaggtca tagcaatgtt ctataccgat
     2641 ggaatgtgct tacttgcctt tttcttcacg acgttttaaa taataagagc cacctaataa
     2701 accagctgga atgcctatca ttggtgttgt gaatgagctt aatacaataa caagtattgt
     2761 taaagcaatg acgttatacc aagttacagt caaatttttc aaatcctcat atgattgttt
     2821 tactaattct ctaaatttca tgattcaatc tctccttttt tataaatctt tagattgtca
     2881 aattaagctg gacaagttta tcatcttcga tgaaactcat aaatacttcc aatggtgttc
     2941 tataacctaa agattttcta ggatgatgat tgattttttg agcaacactg atgatatgat
     3001 catccgttac taaattgaaa tctagttctt tatctaatcc actgcgtcta attattccgt
     3061 tagaatgttc atttaaacct cgctgtgatg gcgtgcctgg atcagcaaaa aatatagata
     3121 tatcgtgaca gttacatatt gttttccagt ttgagaactc cttaccacaa tcaaaagtaa
     3181 ttgatttaaa tatatgagaa gataaaatga aaaggtgcaa gcagtattaa gaatttaggt
     3241 agcaagaaaa caagaagtgg tacatacgat gctaacctaa aaagaattgg agattaaaat
     3301 gattattaac cttgaaacac aacaaataca ttttgattta gaacatgaaa ttccctattt
     3361 tcaagcacct gagaaaaata ggatacgctt agatattgat gttctcaata aaaagcaaat
     3421 ttctaatatt attaatgtta tatttaataa ccaatcaaag acgaaatgta catttttgtc
     3481 tgaat
//
LOCUS       DAXWFK010000198         3461 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.199, whole genome shotgun sequence.
ACCESSION   DAXWFK010000198 DAXWFK010000000
VERSION     DAXWFK010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.199"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(90..791)
                     /locus_tag="ACGEXS_002416"
     CDS             complement(90..791)
                     /locus_tag="ACGEXS_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     ATP-binding protein"
                     /protein_id="HFF8013398.1"
                     /translation="MIELKHVTFGYNKKQMVLQDINITIPDGENVGILGESGCGKSTL
                     ASLVLGLFKPVKGEIYLSDNAVLPIFQHPLTSFNPDWTIETSLKEALYYYRGLTDNTA
                     QDQLLLQHLSTFELNAQLLTKLPSEVSGGQLQRFNVMRSLLAQPRVLICDEITSNLDV
                     IAEQNVINILKAQTITNLNHFIVISHDLSVLQRLVNRIIVLKDGMIVDDFAIEELFNV
                     DRHPYTKELVQAFSY"
     gene            complement(784..1557)
                     /locus_tag="ACGEXS_002417"
     CDS             complement(784..1557)
                     /locus_tag="ACGEXS_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFF8013399.1"
                     /translation="MSLIDIQNLTIKNTSEKSLIKGIDLKIFSQQINALIGESGAGKS
                     LIAKALLEYLPFDLSCTYDSYQFDGENVSRLSQYYGHTIGYISQNYAESFNDHTKLGK
                     QLTAIYRKHYKGSKEEALSKVDKALSWVNLQSKDILNKYSFQLSGGQLERVYIASVLM
                     LEPKLIIADEPVASLDALNGNQVMDLLQHIVLEHGQTLFIITHNLSHVLKYCQYIYVL
                     KEGQIIERGNINHFKYEHLHPYTERLIKYRTQLKRDYYD"
     gene            complement(1544..2374)
                     /gene="nikC"
                     /locus_tag="ACGEXS_002418"
     CDS             complement(1544..2374)
                     /gene="nikC"
                     /locus_tag="ACGEXS_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel transporter permease"
                     /protein_id="HFF8013400.1"
                     /translation="MHKIFSKNNLIFFVFVAFIFVVIVLQFFVSSENATKVNLSQTFE
                     PISWLHLLGTDDYGRDLFTRIIIGARSTLFVTVLTLIAIVVIGVTLGLFAGYKKGWIE
                     RLVLRFIDVGLSIPEFIIMIALASFFQPSLWNLVISITLIKWMNYTRLTRSIVNSEMN
                     KPYIKMAQLFHVPTRTILIRHLTPKIIPAIIVLMVVDFGKIILYISSLSFIGLGAQPP
                     TPEWGAMLQQGRDFISSHPIMLIAPASVIAITILIFNLTGDALRDRLLKQRGEYDESH
                     "
     gene            complement(2367..3353)
                     /gene="nikB"
                     /locus_tag="ACGEXS_002419"
     CDS             complement(2367..3353)
                     /gene="nikB"
                     /locus_tag="ACGEXS_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease"
                     /protein_id="HFF8013401.1"
                     /translation="MFIIKSMLYRLMQMIVVLFVISTLTFILMKLSPGNPVDKILHLD
                     VAQVSTEQINATKDKLGLNDSLLVQWWHWMNHLLHFNLGKSFESKEPVTQILFNYAPI
                     TLLISFSTLVVSLCISIPLGIIAAKRFHKWTDKVIRVISTLSISLPAFFIGIILLFIV
                     TNLMNIDSVILSQFILPVITLSLGMCAYIIRLVRSNLLMLLQSNIVQASRLRGMNERY
                     ILIHDLLKPTILPIIPLLGISLGSLIGGTVVIENLFDIPGIGYLLMDSIKSRDYPVIQ
                     GCVLFIGFFVVIINTIADLLTLLLDPKQRLQLGNPKIKTNTPLISESSDRHA"
BASE COUNT         1214 a          607 c          491 g         1149 t
ORIGIN      
        1 aagaaaataa aatagataag tgtctagatt aaaatacttg atttatctat attttataac
       61 aagtctagaa ttatcgcatt cttaaataac taatatgaaa atgcttgcac taattctttt
      121 gtataagggt gtctatcaac attaaataat tcctctattg caaaatcatc gactatcatg
      181 ccatccttaa gaacgataat tctattaact aagcgttgta acacggataa atcatgagaa
      241 ataacgataa aatgatttaa gttcgtaatc gtttgcgctt ttaatatatt gattacattt
      301 tgttcagcta taacatctaa atttgaagtt atctcatcac atattaaaac gcgaggctgt
      361 gctaataacg aacgcatgac attaaatctt tgtaattgtc cgccactcac ttcgcttggt
      421 aatttagtca ataattgcgc gtttaactca aaagtagata aatgttgtaa taataattga
      481 tcctgtgcag tattatcagt tagacctctg taataatata acgcttcttt taatgaggtc
      541 tcaatcgtcc aatcagggtt aaagctagtt aaagggtgtt ggaaaatcgg taacacagca
      601 ttgtcactta agtaaatctc tcctttaaca ggtttaaaca agccaagaac caatgaagcg
      661 agcgtacttt taccacagcc actttcgcct aaaataccaa cattttctcc atcaggtata
      721 gtaatattga tatcttgtag caccatctgc tttttattat aaccaaaagt cacatgtttt
      781 aactcaatca tagtaatccc tctttaattg tgttctatat ttaattagac gttcagtata
      841 cggatgcaaa tgctcatact tgaaatgatt aatattacct cgttcaatga tttgaccttc
      901 ttttaaaaca taaatgtact gacaatattt caatacatga cttaagttat gtgtgataat
      961 aaataatgtt tgaccatgtt ctaatacaat atgctgtaat aaatccatca cttgattacc
     1021 gttcaaagca tccaatgatg caactggttc gtctgcaatg attaatttag gctccaacat
     1081 gagaacgctt gctatgtata cgcgttcaag ttggccccca gaaagttgga aactatattt
     1141 atttaatata tctttgcttt gtaaattaac ccacgacaaa gccttatcaa ctttggacaa
     1201 agcctcttct ttactacctt tataatgctt acgataaatc gcagttaact gtttacctaa
     1261 tttagtatgg tcgttaaaac tttctgcata attttgagaa atatagccaa ttgtatgacc
     1321 ataatattga ctcaatctac taacattttc cccatcaaat tggtacgaat catacgtgca
     1381 gcttaaatca aatggtaaat attcaagtaa agctttagca atcaaacttt ttccagcgcc
     1441 gctctctcca atcaaggcat taatctgttg actaaaaatt ttcaaatcaa tccctttaat
     1501 aagagatttc tcactagtat tctttattgt taaattttgt atatcaatga gactcatcat
     1561 attcaccccg ttgtttcagc aatctatctc ttagtgcatc accggttaaa ttaaaaatta
     1621 aaatagttat agcaatgact gaagcaggtg caatcaacat aattggatga gacgaaataa
     1681 aatcacgacc ttgttgcaac atagcgcccc actctggtgt tggcggttgt gcacctaacc
     1741 caataaatga tagtgaactt atatatagaa tgattttacc gaaatcaacg accatcaaaa
     1801 cgataatagc cggtataatt ttaggtgtta aatgacgtat taatattgtt cttgttggta
     1861 catgaaataa ttgtgccatt tttatataag gcttattcat ttcgctatta actatacttc
     1921 tagtcaacct tgtgtaattc atccatttta ttaatgtaat tgagataact aaattccata
     1981 aagatggttg aaaaaaactt gctaaagcaa tcatgatgat aaattctgga atacttagac
     2041 caacatcaat aaaccttaac actaatcgtt caatccaccc ttttttgtat ccggcaaata
     2101 gacctagtgt aacacctatg acaacgatag ctattaatgt taaaacagta acaaacaatg
     2161 ttgaacgtgc accgataata attcgggtaa ataaatctct cccataatca tcagttccta
     2221 ataaatgcaa ccaactaata ggttcaaaag tttgtgataa attgactttg gttgcatttt
     2281 cactactgac aaagaattgc agtacaatta ccacaaaaat aaatgcaacg aatacaaaaa
     2341 atatcaggtt attctttgaa aatattttat gcatgacggt cactactttc tgatatcaat
     2401 ggtgtattgg ttttgatttt tggatttcct aattgtaaac gctgcttcgg atcaagtaat
     2461 aacgttaata aatcagcaat cgtattgata ataacaacga agaagccaat aaataacacg
     2521 catccttgaa taacaggata atctcgagat ttaatactat ccattaatag ataaccaata
     2581 ccaggtatat caaataaatt ttcaatcact acagtaccac ctattagact gccaagtgaa
     2641 atccctagta atgggataat cggcaaaatt gttggtttta gtaaatcatg aattaaaata
     2701 taacgttcat tcataccgcg taatcttgat gcttgtacga tattactttg caataacatc
     2761 aataaattag aacgcactaa acgaatgatg tatgcacaca tacctaaaga tagcgtgatt
     2821 acaggtaata taaactgact tagtataacg ctatctatat tcattaaatt tgtgacaata
     2881 aataataaaa taataccgat aaagaacgct ggtaaactaa tcgatagtgt tgagatcact
     2941 ctaatcactt tatccgtcca cttatgaaat cgtttggctg ctataatgcc gagcggtata
     3001 gatatgcata acgacactac taatgttgaa aatgatatga gtaatgttat gggtgcatag
     3061 ttgaataata tctgtgttac cggttctttt gattcaaaac tttttcctaa attaaaatgt
     3121 aataaatgat tcatccaatg ccaccactgt accaataaag aatcatttaa tcccaattta
     3181 tctttggttg catttatttg ttccgtcgac acttgtgcta catcaagatg taatatttta
     3241 tcaacaggat tgcctggtga taatttcatt aaaatgaatg taagtgtaga aataacaaat
     3301 aaaacaacta tcatttgcat cagtctatac aacatagact ttattatgaa cataatagtc
     3361 cccctccttg tgtaagttac taacactttc tttttacatg agaatggcgc atgtatatgc
     3421 aacttacata ttaagaacta acgttcatta tagtattatc c
//
LOCUS       DAXWFK010000199         3441 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.200, whole genome shotgun sequence.
ACCESSION   DAXWFK010000199 DAXWFK010000000
VERSION     DAXWFK010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.200"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..1118
                     /gene="vwb"
                     /locus_tag="ACGEXS_002420"
     CDS             <1..1118
                     /gene="vwb"
                     /locus_tag="ACGEXS_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="von Willebrand factor binding protein Vwb"
                     /protein_id="HFF8013402.1"
                     /translation="EFLKEIEDIKKDNPELKDFNEEEQLKCDLELNKLENQILMLGKT
                     FYQNYRDDVESLYSKLDLIMGYKDEERANKKAVNKRMLENKKEDLETIIDEFFSDIDK
                     TRPNNIPVLEDEKQEVKNHKNMAQLKSDTEAAKSDESKRSKRSKRSLNTQNHKPASQE
                     VSEQQKAEYDKRAEERKARFLDNQKIKKTPVVSLEYDFEHKQRIDNENDKKLVVSAPT
                     KKPTSPTTYTETTTQVPMPTVERQTQQQIIYNAPKQLAGLNGESHDFTTTHQSPTTSN
                     HTHNNVVEFEETSALPGRKSGSLVGISQIDSSHLTEREKRVIKREHVREAQKLVDNYK
                     DTHSYKDRINAQQKVNTLSEGHQKRFNKQINKVYNGK"
     gene            1469..2491
                     /gene="emp"
                     /locus_tag="ACGEXS_002421"
     CDS             1469..2491
                     /gene="emp"
                     /locus_tag="ACGEXS_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular matrix protein-binding adhesin
                     Emp"
                     /protein_id="HFF8013403.1"
                     /translation="MKKKLLVLTMSTLFATQIMNSNHAKASVTESVDKKFVVPESGIN
                     KIIPAYDEFKNSPKVNVSNLTDNKNFVASEDKLNKIADSSAASKIVDKNFVVPESKLG
                     NIVPEYKEINNRVNVATNNPASQQVDKHFVAKGPEVNRFITQNKVNHHFITTQTHYKK
                     VITSYKSTHVHKHVNHAKDSINKHFIVKPSESPRYTHPSQSLIIKHHFAVPGYHAHKF
                     VTPGHASIKINHFCVVPQINSFKVIPPYGHNSHRMHVPSFQNNTTATHQNAKVNKAYD
                     YKYFYSYKVVKGVKKYFSFSQSNGYKIGKPSLNIKNVNYQYAVPSYSPTHYVPEFKGS
                     LPAPRV"
     gene            2826..3296
                     /locus_tag="ACGEXS_002422"
     CDS             2826..3296
                     /locus_tag="ACGEXS_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013404.1"
                     /translation="MKRKVLVLTMGVICATQLWHSNHANALVTESGANDTKQFTEIVS
                     EEKVITVEHAQINIFQSNSNSNLMEFNILTMGGKSGAMVGYSEIDSSHFTDRDKRVIR
                     RDHVKEAQSLVENYKDTQSADARMKAKQKVNTLSKPHQNYFNKQIDKVYNGLQR"
BASE COUNT         1430 a          506 c          524 g          981 t
ORIGIN      
        1 aggagttctt aaaagaaatt gaagatataa aaaaagataa ccctgaattg aaagacttta
       61 atgaagagga gcaattaaag tgcgacttag aattaaacaa attagaaaat cagatattaa
      121 tgttaggtaa aacattttat caaaactata gagatgatgt tgaaagttta tatagtaagt
      181 tagatttaat tatgggatat aaagatgaag aaagagcaaa taaaaaagca gttaacaaaa
      241 ggatgttaga aaataaaaaa gaagacttag aaaccataat tgatgaattt tttagtgata
      301 tagataaaac aagacctaat aatattcctg ttttagaaga tgaaaaacaa gaagtgaaaa
      361 atcataaaaa tatggctcaa ttaaaatctg acactgaagc agcaaaaagt gatgaatcaa
      421 aaagaagcaa gagaagtaaa agaagtttaa atactcaaaa tcacaaacct gcatctcaag
      481 aagtttctga acaacaaaaa gctgaatatg ataaaagagc agaagaaaga aaagcgagat
      541 ttttggataa tcaaaaaatt aagaaaacac ctgtagtgtc attagaatat gattttgagc
      601 ataaacaacg tattgacaac gaaaacgaca agaaacttgt ggtttctgca ccaacaaaga
      661 aaccaacatc accgactaca tatactgaaa caacgacaca ggtaccaatg cctacagttg
      721 agcgtcaaac tcagcaacaa attatttata atgcaccaaa acaattggct ggattaaatg
      781 gtgaaagtca tgatttcaca acaacgcatc aatcaccaac aacttcaaat cacacgcata
      841 ataatgttgt tgaatttgaa gaaacgtctg ctttacctgg tagaaaatca ggatcactgg
      901 ttggtataag tcaaattgat tcttctcatc taactgaacg tgagaagcgt gtaattaagc
      961 gtgaacacgt tagagaagct caaaagttag ttgataatta taaagataca catagttata
     1021 aagaccgaat aaatgcacaa caaaaagtaa atactttaag tgaaggtcat caaaaacgtt
     1081 ttaataaaca aatcaataaa gtatataatg gcaaataatt aatgcatggc tgcaaagcaa
     1141 ataatgagtt tgtcgtaaaa ataacaacat tttaaactag caataaataa tatcaaagtc
     1201 atcatttcaa tgatgcaatc tagtatagtc cacattctaa acaggtgtgg actattactt
     1261 ttttcacttt atattacgaa aaaattatta tgcttaacta tcaatatcaa taattaattt
     1321 taagctgaaa aacaataaaa atgttaagac aacgtttact tcaagttaat tattatactg
     1381 aaaattctgg tatataatgc tgttagtgaa tataacaggg aaattatatt ggttataata
     1441 ttgagtctat ataaaggaga aataacagat gaaaaagaaa ttattagttt taactatgag
     1501 cacgctattt gctacacaaa ttatgaattc aaatcacgct aaagcatcag tgacagagag
     1561 tgttgacaaa aaatttgtag ttccagaatc aggaattaat aaaattattc cagcttacga
     1621 tgaatttaag aattcgccaa aagtaaatgt tagtaattta actgacaata aaaactttgt
     1681 agcttctgaa gataaattga ataagattgc agattcatcg gcagctagta aaattgtaga
     1741 taaaaacttt gtcgtaccag aatcaaagtt aggaaacatt gtgccagagt acaaagaaat
     1801 caataatcgc gtgaatgtag caacaaacaa tccagcttca caacaagttg ataagcattt
     1861 tgttgctaaa ggcccagaag taaatagatt tattacgcaa aacaaagtaa accaccactt
     1921 cattactacg caaacccact acaagaaagt tattacttca tacaaatcaa cacatgtaca
     1981 taaacatgta aatcatgcaa aggattctat taataaacac tttattgtta aaccatcaga
     2041 atcgcctaga tatacacatc catctcaatc tttaattatc aagcatcatt ttgcagttcc
     2101 tggatatcac gcgcataaat ttgttacacc agggcatgct agcattaaaa ttaatcactt
     2161 ttgtgttgtg ccacaaataa atagtttcaa ggtaattcca ccatatggtc acaattcaca
     2221 tcgtatgcat gtaccaagtt tccaaaataa cacaacagca acacatcaaa atgctaaagt
     2281 aaataaagca tatgactata aatacttcta ttcttataaa gtagttaaag gtgtgaagaa
     2341 atatttctca ttttcacaat caaatggtta taaaattggg aaaccatcat taaatatcaa
     2401 aaatgtaaat tatcaatatg ctgttccaag ttatagccct acacactacg ttcctgaatt
     2461 taagggtagc ttaccagcac cacgagtata aaaattggca ctaagtttac gagatatgat
     2521 aaatacctat tattttaaat atagtctaca atctatgtgg ttgtaggctg tattttttgc
     2581 agtttatcaa taaacaccca tcaacaaatt ataccgtttt tctactttga aagttggaag
     2641 taacataatc ttaaataaat atattattaa ttaagataaa tataatactc aagattattg
     2701 ttaatagttt gttcatcgca agttaattat tgtttctaaa atattggtat ataattttca
     2761 atggcgaaga aaacagggtg aaaaagtcgg tttttatatc aaagcaaata agggagcata
     2821 aacaaatgaa aaggaaagta ttagtactaa caatgggtgt aatttgtgca actcaattat
     2881 ggcattctaa tcacgcaaac gcattagtaa cagagagtgg cgcaaatgat actaagcaat
     2941 ttactgaaat tgtatcggaa gagaaagtta taacagttga acatgctcaa attaatattt
     3001 ttcaatctaa tagcaattca aacttgatgg agttcaacat attaacaatg ggcggtaaat
     3061 caggagctat ggttggttat agtgaaattg actcatcaca tttcacagac cgtgacaaac
     3121 gcgttattag acgtgatcat gttaaagaag cacaaagctt agtagagaac tataaagata
     3181 cacaaagtgc tgatgctagg atgaaagcca aacaaaaagt taacacatta agcaaaccgc
     3241 atcaaaacta tttcaataaa caaattgata aggtttataa tggattacaa cgctaatcca
     3301 aagtaaatta taagttatac atctcgtttt taaatgacaa tttatccccg taaatattat
     3361 aaataatctt ttcaaattcc acatagatat agagacacta ataaacctct ttgtctcgat
     3421 atgatagtct gcaacgattc a
//
LOCUS       DAXWFK010000200         3404 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.201, whole genome shotgun sequence.
ACCESSION   DAXWFK010000200 DAXWFK010000000
VERSION     DAXWFK010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.201"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(403..507)
                     /locus_tag="ACGEXS_002423"
     CDS             complement(403..507)
                     /locus_tag="ACGEXS_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013405.1"
                     /translation="MLTNRLTSVNYVKKRLIQNIQRILSKEVLYGEMQ"
     gene            602..1882
                     /gene="sbi"
                     /locus_tag="ACGEXS_002424"
     CDS             602..1882
                     /gene="sbi"
                     /locus_tag="ACGEXS_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin-binding protein Sbi"
                     /protein_id="HFF8013406.1"
                     /translation="MKNKYISKLLVGAATITLATMISNGEAKASENTQQTSTKHQTTQ
                     NNYVTDQQKAFYQVLHLKGITEEQRNQYIKTLREHPERAQEVFSESLKDSKNPDRRVA
                     QQNAFYNVLKNDNLTEQEKNNYIAQIKENPDRSQQVWVESVQSSKAKERQNIENADKA
                     IKDFQDNKAPHDKSAAYEANSKLPKDLRDKNNRFVEKISIEKAIVRHDERVKSANDAI
                     SKLNEKDSIENRRLAQREVNKAPMDVKEHLQKQLDALVAQKDAEKKVAPKVEAPQIQS
                     PQIEKPKVESPKVEVPQSKLLGYYQSLKDSFNYGYKYLTDTYKSYKEKYDTAKYYYNT
                     YYKYKGAIDQTVLTVLGSGSKSYIQPLKVDDKNGYLAKSYAQVRNYVTESINTGKVLY
                     TFYQNPTLVKTAIKAQETASSIKNTLSNLLSFWK"
     gene            2418..3347
                     /gene="hlgA"
                     /locus_tag="ACGEXS_002425"
     CDS             2418..3347
                     /gene="hlgA"
                     /locus_tag="ACGEXS_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgAB subunit A"
                     /protein_id="HFF8013407.1"
                     /translation="MIKNKILTATLAVGLIAPLANPFIEISKAENKIEDIGQGAEIIK
                     RTQDITSKRLAITQNIQFDFVKDKKYNKDALVVKMQGFISSRTTYSDLKKYPYIKRMI
                     WPFQYNISLKTKDSNVDLINYLPKNKIDSADVSQKLGYNIGGNFQSAPSIGGSGSFNY
                     SKTISYNQKNYVTEVESQNSKGVKWGVKANSFVTPNGQVSAYDQYLFAQDPTGPAARD
                     YFVPDNQLPPLIQSGFNPSFITTLSHERGKGDKSEFEITYGRNMDATYAYVTRHRLAV
                     DRKHDAFKNRNVTVKYEVNWKTHEVKIKSITPK"
BASE COUNT         1333 a          605 c          482 g          984 t
ORIGIN      
        1 tttatgagct agaaaggcgc ccaactctcc ctgtttgtta actttcgcct cgaaagtttc
       61 tatgttggga ccctatgcat tagtagcgaa gctatctaat gtgttccttt attttctgcg
      121 aattttatga gctagaaagg cttatgcagt tgacgtttta cgtccaactg cgttcctccg
      181 tcttcttcaa atttatttgt tagaaaggca cccaactctc cctgtttgtt aactttcgcc
      241 tcgaaagttt ctatgttaga accctatgca tgagttgcga agctatctaa tgtcgtgaac
      301 taattatata gaagaaaaag tgcatcaatg acaaattaaa tgagatttct actctaccaa
      361 actctcttcg aaagacaatt ttctcctcta tttattagca aactattgca tttctccata
      421 tagtacttcc ttacttaaaa tacgctgaat gttctgaatt aaacgctttt ttacatagtt
      481 aacactagtt aatctattag ttaacattag ttaataatta gttaatttcc atttgtattc
      541 tcatgtgata aattctaaaa gcatacaata aatttaatat gtaaaaagaa agggaataca
      601 catgaaaaat aaatatatct cgaagttgct agttggggca gcaacaatta cgttagctac
      661 aatgatttca aatggggaag caaaagcgag tgaaaacacg caacaaactt caactaagca
      721 ccaaacaact caaaacaact acgtaacaga tcaacaaaaa gctttttatc aagtattaca
      781 tctaaaaggt atcacagaag aacaacgtaa ccaatacatc aaaacattac gcgaacaccc
      841 agaacgtgca caagaagtat tctctgaatc acttaaagac agcaagaacc cagaccgacg
      901 tgttgcacaa caaaacgctt tttacaatgt tcttaaaaat gataacttaa ctgaacaaga
      961 aaaaaataat tacattgcac aaattaaaga aaaccctgat agaagccaac aagtttgggt
     1021 agaatcagta caatcttcta aagctaaaga acgtcaaaat attgaaaatg cggataaagc
     1081 aattaaagat ttccaagata acaaagcacc acacgataaa tcagcagcat atgaagctaa
     1141 ctcaaaatta cctaaagatt tacgtgataa aaacaaccgc tttgtagaaa aaatttcaat
     1201 tgaaaaagca atcgttcgtc atgatgagcg tgtgaaatca gcaaatgatg caatctcaaa
     1261 attaaatgaa aaagattcaa ttgaaaacag acgtttagca caacgtgaag ttaacaaagc
     1321 acctatggat gtaaaagagc atttacagaa acaattagac gcattagttg ctcaaaaaga
     1381 tgctgaaaag aaagtggcgc caaaagttga ggctcctcaa attcaatcac cacaaattga
     1441 aaaacctaaa gtagaatcac caaaagttga ggttcctcaa tctaaattat taggttacta
     1501 ccaatcatta aaagattcat ttaactatgg ttacaagtat ttaacagata cttataaaag
     1561 ctataaagaa aaatatgata cagcaaagta ctactataat acgtactata aatacaaagg
     1621 tgcgattgat caaacagtat taacagtact aggtagtggt tctaaatctt acatccaacc
     1681 attgaaagtt gatgataaaa acggctactt agctaaatca tatgcacaag taagaaacta
     1741 tgtaactgag tcaatcaata ctggtaaagt attatatact ttctaccaaa acccaacatt
     1801 agtaaaaaca gctattaaag ctcaagaaac tgcatcatca atcaaaaata cattaagtaa
     1861 tttattatca ttctggaaat aatcaatcaa aaatatcttc tctagtttta catcattttt
     1921 taaataattt tcgtaacaaa ccgtgattaa aaagaaccgt tgattctcaa tcgaatctac
     1981 ggttcttttt tcattttcca tcaattaaat gcttcttcgc tatttgtcag cccacttttt
     2041 tacctgcaac ttgttaaata atccttacat cgttaacgaa tagttcatca tttagttgaa
     2101 tcagctcaac tttattaact tcatattttc acaaactatt gcgcaattca ttccttttcc
     2161 actacaagca ccataattaa acaacaattc aataaaataa gacttgcaaa gcatagttat
     2221 gtagctatat aaacgcctgc gaccaataaa tcttttaaac ataacataat gcaaaaacat
     2281 catttaacaa tgctaaaaat gtctcttcaa tacatgttga tagtaattaa cttttaacga
     2341 acagttaatt cgaaaacgct tacaaatgga ttattatata tatgaactta aaattaaata
     2401 gaaagaaagt gatttctatg attaaaaata aaatattaac agcaacttta gcagttggtt
     2461 taatagcccc tttagccaat ccatttatag aaatttctaa agcagaaaat aagatagaag
     2521 atatcggcca aggtgcagaa atcatcaaaa gaacacaaga cattactagc aaacgattag
     2581 ctataactca aaacattcaa tttgattttg taaaagataa aaaatataac aaagatgccc
     2641 tagttgttaa gatgcaaggc ttcattagct ctagaacaac atattcagac ttaaaaaaat
     2701 atccatatat taaaagaatg atatggccat ttcaatataa tatcagtttg aaaacgaaag
     2761 actctaatgt tgatttaatt aattatcttc ctaaaaataa aattgattca gcagatgtta
     2821 gtcagaaatt aggctataat atcggcggaa acttccaatc agcgccatca atcggaggca
     2881 gtggctcatt caactactct aaaacaatta gttataatca aaaaaactat gttactgaag
     2941 tagaaagtca gaactctaaa ggtgttaaat ggggagtgaa agcaaattca ttcgttacac
     3001 cgaatggtca agtatctgca tatgatcaat acttatttgc acaagaccca actggtccag
     3061 cagcacgaga ctatttcgtc ccagataatc aactacctcc tttaattcaa agtggcttta
     3121 atccatcatt tattacaaca ttgtcacacg aaagaggtaa aggtgataaa agcgagtttg
     3181 aaatcactta cggcagaaac atggatgcta catatgctta cgtgacaaga catcgtttag
     3241 ccgttgatag aaaacatgat gcttttaaaa accgaaacgt tacagttaaa tatgaagtga
     3301 actggaaaac acatgaagta aaaattaaaa gcatcacacc taagtaaaca gttcaatcat
     3361 cttaaaaaat cctgggacac ttcatacttg tctcaggatt tttt
//
LOCUS       DAXWFK010000201         3387 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.202, whole genome shotgun sequence.
ACCESSION   DAXWFK010000201 DAXWFK010000000
VERSION     DAXWFK010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.202"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..464
                     /locus_tag="ACGEXS_002426"
     CDS             <1..464
                     /locus_tag="ACGEXS_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HFF8013408.1"
                     /translation="STNNQSSKTQLTQKDENKNEDKPEEKSETATDEDLQSTEEVPAN
                     ENTENNQHEIDEITTKDQSDDDINTPNVAEDKSQDDLKDDLKEKQQSSNHHQSTQPKT
                     SPSTETNTQQSFANCKQLRQVYPNGVTADHPAYRPHLDRDKDKRACEPDKY"
     gene            545..1540
                     /locus_tag="ACGEXS_002427"
     CDS             545..1540
                     /locus_tag="ACGEXS_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFF8013409.1"
                     /translation="MSNQFKSEEERRQWEQFQAFQNQQNQQNQQYGQKKSKKGWFWGC
                     GGCLVLFILIIIGISACTAGITGNLGGNSSKETNKTHKIGETVKNGDLEVTVNSVETM
                     KSVGPSLAPTNAKGIFVVADVTIKNKGKEALTIDSSMFKLKSGDKTFEADNTGSMSAN
                     QSDNGSIENSFFLQRINPDSTAQGKIVFDVSENIANAKDKKLEVISSLFSVKKITFDL
                     SDAKKTSKAKKDKQDTEVAVASSNSDNVSYEASATTPATTSSADTDSEDSEKSSKDED
                     KQNASKSDKSSVEKSESNEETAPVEPMPHSKPTTSEAPPSQNIHNEDSMYDASTE"
     gene            1616..2242
                     /locus_tag="ACGEXS_002428"
     CDS             1616..2242
                     /locus_tag="ACGEXS_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013410.1"
                     /translation="MKFKAIVAITLSLSLLTACGANQHKENSSKSNDTNKKTQQTDNT
                     TQSNTEKQMTPQEAEDIVRNDYKARGVNEYQTLNYKTNLERSNEHEYYVEHLVRDAVG
                     TPLKRCAIVNRHNGTIINIFDDMSEKDKEEFEAFKKRSPKYNPGMNNHDETDGESEDI
                     QHHDIDNNKAIQNDIPDQKVDDKNDKNAVNKEEKHDNGANNSEETKVK"
     gene            2283..2627
                     /locus_tag="ACGEXS_002429"
     CDS             2283..2627
                     /locus_tag="ACGEXS_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3969 family protein"
                     /protein_id="HFF8013411.1"
                     /translation="MITFENIQQLEKYTLMTMHGLFNQLKLGIISIENAEHTLFTPYM
                     LETLSSLGVKDSIVDLIHKGTELEDFAAFNLSIEDTVTVCLQRTEELLKQYKNVEFND
                     KILINWRIIQEK"
     gene            2725..3276
                     /locus_tag="ACGEXS_002430"
     CDS             2725..3276
                     /locus_tag="ACGEXS_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013412.1"
                     /translation="MEKNEYIAKYNEYSQLLDATYSQAVAYLLSKYGAVTDDYYKEKS
                     YTRFLNGEIKSISKGKYTRASEGLYCHHISEDKFQNLSDLRFISKFKYSYDVQKKENL
                     VYCDLIEHLILHAIITKESHGQFGVAGLCQMIKPTVIDWYIGEYNPKPAWMQATKARA
                     YLPGILVEKLLIKIDDMLKGIEI"
BASE COUNT         1386 a          539 c          571 g          891 t
ORIGIN      
        1 attccactaa taatcaatca tcaaaaacgc aattaactca aaaagatgaa aataaaaatg
       61 aagataagcc tgaggaaaaa tcagaaacag caacagatga ggatttacaa tcaaccgaag
      121 aagtacctgc aaatgaaaat actgaaaata atcaacatga aattgatgaa ataacaacaa
      181 aagatcaatc agacgatgat attaacacac caaacgttgc agaagataaa tcacaagacg
      241 acttgaaaga tgatttaaaa gaaaagcaac aatcaagtaa ccatcatcaa tccacgcaac
      301 ctaagacctc accatcaact gaaacaaaca cgcaacaatc atttgctaat tgtaagcaac
      361 ttagacaagt atatccgaat ggtgtcactg ccgatcatcc agcatatcga ccacatttag
      421 atagagataa agataaacgt gcatgtgaac ctgataaata ttaaacaaca agcgaattga
      481 attcaaattg tatttagctt tatgcactaa tcacatagta aataatgagg gagatttttt
      541 aggcatgagc aatcaattca aaagcgaaga agagcgaaga caatgggaac aattccaagc
      601 tttccaaaat caacaaaacc aacagaacca gcaatacgga caaaagaaat ctaaaaaagg
      661 atggttctgg ggctgtggtg gttgtctagt attatttatt ttaattatca tcggtatttc
      721 agcttgtaca gctggtatta caggtaacct tggcggaaat agttctaaag aaacgaacaa
      781 aacccataaa atcggtgaaa ctgttaaaaa tggcgacctt gaagtcactg taaattcagt
      841 ggaaactatg aaatctgtag gaccatctct tgcaccaaca aacgctaaag gtatatttgt
      901 cgttgctgat gtgacgatta aaaacaaagg taaagaagcg ttaacaattg atagttcaat
      961 gtttaagcta aaatccggtg ataaaacatt tgaagcagat aatacaggtt caatgtctgc
     1021 taatcaaagt gacaatggta gtatagaaaa ttcatttttc ttacagcgta taaatccaga
     1081 tagcactgct caaggtaaaa ttgttttcga tgtgtcagaa aacatagcca acgcaaaaga
     1141 taaaaaatta gaagttattt ctagtttatt tagcgtcaag aagattacat ttgatttatc
     1201 cgatgctaaa aaaacatcaa aagctaaaaa agacaagcaa gatacagaag tagctgttgc
     1261 gagttcaaat agcgataatg taagttatga agcttcggct actacacctg ctacaacttc
     1321 tagtgcggat actgattctg aagatagcga aaagtctagt aaagatgagg ataagcagaa
     1381 tgcgtctaaa agtgataaat ctagtgtaga aaaaagtgaa tctaatgagg aaactgctcc
     1441 tgtagagccc atgccccata gcaaacctac cactagtgaa gcaccaccta gccaaaatat
     1501 tcacaatgaa gatagcatgt acgacgcttc aacagaataa aatttcaggg tagctcggct
     1561 acccttcttt tacggaaaaa ttaattatac ataatcaaat caaggagata aaaaaatgaa
     1621 attcaaagct atcgttgcaa tcacattatc attgtcacta ttaactgcct gtggtgctaa
     1681 tcaacataaa gaaaatagta gtaaatcaaa tgacactaat aaaaagacgc aacaaactga
     1741 caacactaca cagtcaaata cagaaaagca aatgacacca caagaagccg aagatatagt
     1801 tcgaaacgat tacaaagcaa gaggcgttaa tgaatatcaa acattaaatt ataaaacaaa
     1861 tcttgaacga agcaatgaac atgaatatta tgttgaacat ctagtccgcg atgcagttgg
     1921 cacaccatta aaacgttgtg ctattgttaa tcgacacaat ggcacaatta ttaatatttt
     1981 tgatgatatg tcagaaaaag acaaagaaga atttgaagca tttaaaaaga gaagccctaa
     2041 atacaatcca ggtatgaaca atcatgatga aacagatggt gagtcagaag acattcaaca
     2101 tcatgacatt gataataaca aagcaattca aaatgacata ccagatcaaa aagtcgacga
     2161 taaaaatgat aaaaatgctg ttaataaaga agaaaaacat gataatgggg caaataattc
     2221 tgaagaaact aaagttaaat aatggcatac tttgattaat cgtaattttt aggagaaacg
     2281 ctatgataac atttgagaat atacaacaac ttgagaaata caccttaatg actatgcatg
     2341 gtctttttaa tcaacttaaa ctcggcatta tttcaatcga aaacgcagag catacgcttt
     2401 ttacacctta tatgttggaa acgctctctt ccctaggcgt gaaagacagc attgtcgatt
     2461 taattcataa agggactgaa ttagaagact ttgcggcatt taatttatca attgaagaca
     2521 cagttacagt ctgtttacaa agaactgaag aactattaaa acaatacaaa aatgtggaat
     2581 tcaatgacaa aatattaatc aattggcgta ttatacaaga gaaatagaca tataaaagtc
     2641 gaatgtaact acgtgagtat tgattttatt ctttgtaaat tacaagcatt tcatattata
     2701 aagtttgaaa agaggtatat tgaaatggag aaaaatgaat atatagctaa atataatgaa
     2761 tatagtcaat tattagacgc tacatactcg caagctgtag catacctttt aagtaaatat
     2821 ggcgctgtaa ccgatgatta ttataaagaa aaatcataca cgcgattttt aaatggagaa
     2881 atcaaaagta tttcaaaagg aaaatacact agagctagcg aaggattata ttgccatcat
     2941 ataagcgagg acaaattcca aaatctatct gatctaagat tcatttccaa atttaagtac
     3001 tcatacgacg ttcaaaagaa agaaaactta gtgtactgtg atctaatcga gcatttaatt
     3061 ttacatgcaa ttattacaaa agaatcccat ggccaatttg gtgtagctgg attatgtcaa
     3121 atgatcaaac caacagtcat tgattggtac attggcgaat ataatccaaa accagcatgg
     3181 atgcaagcca ccaaagcacg tgcctatttg cctggaatat tagtagagaa attactcatt
     3241 aaaattgacg atatgttaaa aggaatagaa atataagatt tccttgagtc tagataaatg
     3301 attaatgtag atttattttt tgctgttgag attttgttat agatgtttaa acctgtaatt
     3361 aaatatattt tataaaatag accacgc
//
LOCUS       DAXWFK010000202         3347 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.203, whole genome shotgun sequence.
ACCESSION   DAXWFK010000202 DAXWFK010000000
VERSION     DAXWFK010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.203"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(402..1223)
                     /locus_tag="ACGEXS_002431"
     CDS             complement(402..1223)
                     /locus_tag="ACGEXS_002431"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFF8013413.1"
                     /translation="MKFKIPENFNDLSLRDIFQQLKVPKKDLHHLNMSKDITINDKPA
                     RLMDKVHTGDDVFVPTIDEKSNYVPSYRYAQIKYEDDDMAIVMKPKGVKTHPNDLKES
                     NTLMNHVIYTIDSDYVEPIHRLDQETVGLLIVAKNPLMKKILDRMLEDNDITRIYKAN
                     VKALLPLKPQTIDMPIGKDKFHSNKRRVSPTGQRAITHILTSKMIKEAVCQLEIKLDT
                     GRTHQIRVHLAEIGHPVIGDPLYGDSTLRQLELESYKIEFVHPLTKEVISVSLDD"
     gene            1386..2498
                     /locus_tag="ACGEXS_002432"
     CDS             1386..2498
                     /locus_tag="ACGEXS_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HFF8013414.1"
                     /translation="MEQRTRLALLKEIAEFLNEETEMYSMTQGALKYLIEGSNFTTGW
                     IFFINSVGEHELVSHVALPQSLTADHCHYIKDGSCWCVKAFNQRRLMKASNIVNCSRI
                     NLASKAFPSQNDNITHHATVPLKSGQEQFGILNVASPNTEIYSDEDLELLESVAFQLG
                     SAIKRIYLTDREKEAAKINERNRLARDLHDSVNQMLFSVKLTAHAAYGMSNESIAKQA
                     FKTIEETSQNAVNEMRALIWQLKPVGLEQGLIHALTAYSKLMHIQLNVNVEGLIDLSN
                     EIEENIYRALQECINNVKKHADTNKMDLTLKQMNDILYIDVIDYGQGFEIDNVQIASS
                     HGINNIKQRVKLLRGKVTFHSQPTKGTQIQFTIPIK"
     gene            2520..3143
                     /locus_tag="ACGEXS_002433"
     CDS             2520..3143
                     /locus_tag="ACGEXS_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFF8013415.1"
                     /translation="MNKVILVDDHYIVRQGLRFLLSTIENIEVLQDFADGETFLEYLK
                     EHEHPDIVLLDLVMPGMNGIEITEYIKAHYPDIKVLVLTSYVDDEHVISAINKGADGY
                     EMKDVEPQQLIETIRRVMNGEKMIHPKAQDVFETVSQKPHYTNKLSKREIEVLREMVK
                     GKTNKEIAETLFVSEKTIKTHVSHIFSKLQVSDRTQAAIYAMENKLI"
BASE COUNT         1204 a          495 c          561 g         1087 t
ORIGIN      
        1 tttttgtcta tgtcaaattg aacttataag tctgatttga tatagagcca tgaaaaggaa
       61 aaacttttca tggagtccgt tttattatag aacctatttt ttatatgatg aattgttgta
      121 catgtattgg ccaaacaacc tgtgataaaa atcttatgat gtcatgtatt gtccatagat
      181 acacgtaacg cagttgaaaa agactacaag tcattatcca aggatgttat aaaatgaata
      241 attactaaaa tatcaaaata taaaatccta agcatcgtaa aaaacaaaaa aaccgaacat
      301 ctgaaaaaat taataatttt ccatctatcc gatttaagtt gatacttgat ttagttacaa
      361 tgcggtattg catgtttaaa tatcatgcaa aactaatcaa attagtcatc caaagaaacg
      421 gaaatgactt ccttagtcaa gggatgcaca aactctattt tgtaactttc aagttctaat
      481 tgtcttaacg ttgaatcacc atataaagga tcaccaataa cagggtgacc aatttcagct
      541 aaatgcacac gaatttgatg agtacgtcca gtatccaact tgatttcaag ttggcacaca
      601 gcttctttta tcatttttga agttaaaatg tgtgtaattg cacgctgtcc agtaggagac
      661 acacgtcgtt tattcgaatg gaatttatct ttaccaattg gcatatcaat cgtttgtggt
      721 tttaaaggta aaagtgcctt aacatttgct ttgtatatcc gcgtaatatc attgtcttct
      781 aacatgcgat caagaatttt tttcattaaa ggatttttag caacaattaa taatcctact
      841 gtttcctggt ccagtcgatg aattggttcg acatagtcac tatcaatagt gtaaatcaca
      901 tgattcatta aagtattgct ttcttttaaa tcattagggt gagtcttaac acctttaggt
      961 ttcattacga ttgccatatc atcgtcttcg tatttaattt gtgcataacg ataacttgga
     1021 acataattac ttttttcatc gatggttgga acaaatacat cgtcgccagt atgcacttta
     1081 tccattaatc gcgcaggttt atcattaata gtaatatctt tagacatatt taaatgatgt
     1141 aaatcttttt taggtacctt aagttgttgg aaaatatctc gtaaacttaa gtcattaaag
     1201 ttttctggta ttttaaattt cataaaatcc tcctatagtc tttaatatca tatcacaaat
     1261 cagttctccg aaatataact attgagtaat caaggccatt ttatcatgtt tacgtctaag
     1321 atttaaaaat ataataactc aatgtataat agttaataca cgtaaaaatg aattggagcg
     1381 atttgatgga acaaaggacg cgactagctt tattaaaaga gattgccgaa tttttaaacg
     1441 aagaaactga aatgtatagc atgacccaag gtgcgttaaa atatttaatt gagggcagta
     1501 atttcacgac aggctggata tttttcatca atagcgtagg tgaacatgaa cttgtatcac
     1561 atgtggctct accacaatct ttgacagcag accactgtca ctatattaaa gatgggtctt
     1621 gttggtgtgt gaaagcattt aatcaacgta ggctaatgaa agcgtcgaat atcgtcaatt
     1681 gttctcgtat taacttagcc tctaaagcat ttcctagcca aaatgacaat attacacatc
     1741 atgcaacggt accacttaaa tcgggtcaag aacaattcgg cattttaaat gtagcgtctc
     1801 caaatactga aatatatagc gatgaagatt tagagttgct agagtctgtc gcatttcaat
     1861 tagggtcagc catcaaacgt atttatttaa cagaccgtga aaaagaagcg gcaaaaatta
     1921 atgaacgtaa tcgtttagct agagatttac atgattcagt aaatcaaatg ttattttctg
     1981 taaaactaac agcacatgct gcgtatggca tgtcaaacga atccatcgct aaacaagcat
     2041 tcaagacgat tgaggaaacg agtcaaaatg ccgttaatga aatgcgtgca ttgatttggc
     2101 aacttaagcc agttggactt gagcaaggtt taattcatgc tttgacagca tatagtaaat
     2161 taatgcatat acaattaaat gtaaatgtag aaggtttaat cgatttatct aatgaaatcg
     2221 aagaaaacat atacagagca ttacaagagt gtattaataa tgttaagaaa catgctgata
     2281 cgaataaaat ggatcttaca ttaaaacaaa tgaacgatat actatatatt gacgtgattg
     2341 attatggtca aggatttgag atcgataatg ttcagatagc ttcatcacac ggtattaata
     2401 acataaaaca aagagttaaa ttattaagag gtaaagtgac ctttcattct cagccaacaa
     2461 agggtacaca aatacaattc acaattccta taaaatagct tgatttaagg agataaccca
     2521 tgaacaaagt aatattagta gatgaccatt atattgtgcg acaaggattg cgatttttat
     2581 tatccacgat tgaaaacata gaagttttac aagactttgc agatggagaa acatttttag
     2641 aatatttaaa agagcatgag caccctgata ttgtgctatt agatttagtg atgcctggca
     2701 tgaatggtat tgaaattacg gaatatatta aggcacatta tccggatatt aaagttttgg
     2761 tattaacaag ttatgttgat gatgaacatg taatttcagc aatcaataaa ggtgctgatg
     2821 gttatgaaat gaaagacgtt gagcctcagc aattaattga aactattaga cgagttatga
     2881 acggtgaaaa aatgatacat cctaaggcac aagatgtatt cgaaacagtt agccaaaaac
     2941 cacactacac gaataagttg tcaaagagag aaattgaagt gttacgtgaa atggttaaag
     3001 gtaaaacaaa taaagagatt gcagaaactt tatttgtatc tgaaaaaaca attaaaacac
     3061 atgtcagtca tatatttagt aaattacaag ttagcgatcg tacacaagca gcaatttatg
     3121 caatggaaaa taagttgatt tagaatgagc ttttaaatat ttgtcgaaaa atagacaatt
     3181 tttaatctga ttttgatgaa ttttttatag cgatagggtt tattatacag ttttctttgt
     3241 aaatttgata tcattataaa agtgtaagtt ttatcaaatc tagtgattat gcataaaatt
     3301 ttacttcatt tactttcgct tcattataaa gtaaacttaa ctcttgt
//
LOCUS       DAXWFK010000203         3290 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.204, whole genome shotgun sequence.
ACCESSION   DAXWFK010000203 DAXWFK010000000
VERSION     DAXWFK010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.204"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(117..467)
                     /gene="rbfA"
                     /locus_tag="ACGEXS_002434"
     CDS             complement(117..467)
                     /gene="rbfA"
                     /locus_tag="ACGEXS_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFF8013416.1"
                     /translation="MSSMRAERVGEQMKKELMDIINNKVKDPRVGFITITDVVLTNDL
                     SQAKVFLTVLGNDKEVENTFKALDKAKGFIKSELGSRMRLRIMPELMYEYDQSIEYGN
                     KIERMIQDLHKQDR"
     gene            complement(853..2970)
                     /gene="infB"
                     /locus_tag="ACGEXS_002435"
     CDS             complement(853..2970)
                     /gene="infB"
                     /locus_tag="ACGEXS_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFF8013417.1"
                     /translation="MSKQRIYEYAKELNLKSKEIIDELKSMNIEVSNHMQALEDDQIK
                     ALDKKFKKEQKNDNKQSTQNNHQKSNNQNQNKGQQKDNKKNQQQNNKGNKGNKKNNRN
                     NKKNNKNNKPQNQPAAPKEIPSKVTYQEGITVGEFADKLNVESSEIIKKLFLLGIVAN
                     INQSLNQETIELIADDYGVEVEEEVVINEEDLSIYFEDEKDDPEAIERPAVVTIMGHV
                     DHGKTTLLDSIRHTKVTAGEAGGITQHIGAYQIENDGKKITFLDTPGHAAFTTMRARG
                     AQVTDITILVVAADDGVMPQTIEAINHAKEAEVPIIVAVNKIDKPTSNPDRVMQELTE
                     YGLIPEDWGGETIFVPLSALSGDGIDDLLEMIGLVAEVQELKANPKNRAVGTVIEAEL
                     DKSRGPSASLLVQNGTLNVGDAIVVGNTYGRIRAMVNDLGQRIKTAGPSTPVEITGIN
                     DVPQAGDRFVVFSDEKQARRIGESRHEASIIQQRQESKNVSLDNLFEQMKQGEMKDLN
                     VIIKGDVQGSVEALAASLMKIDVEGVNVRIIHTAVGAINESDVTLANASNGIIIGFNV
                     RPDSGAKRAAEAENVDMRLHRVIYNVIEEIESAMKGLLDPEFEEQVIGQAEVRQTFKV
                     SKVGTIAGCYVTEGKITRNAGVRIIRDGIVQYEGELDTLKRFKDDAKEVAKGYECGIT
                     IENYNDLKEGDVIEAFEMVEIKR"
     gene            complement(2975..>3290)
                     /locus_tag="ACGEXS_002436"
     CDS             complement(2975..>3290)
                     /locus_tag="ACGEXS_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YlxQ family RNA-binding protein"
                     /protein_id="HFF8013418.1"
                     /translation="SIDQILNFLGLAMRAGKVKTGESVIVNEIKKGNLKLVIVANDAS
                     DNTAKLITDKCKSYKVPFRKFGNRNELGIALGKGERVNVGITDPGFAKKLLSMIDEYH
                     KE"
BASE COUNT          967 a          639 c          420 g         1264 t
ORIGIN      
        1 ttcataaaaa aactggaaaa tgttaactat ttctacacat ctatgtatta actgtttaat
       61 gttaatatac cgatattaag aaattatcaa acattttcca gatacctaac actaaattat
      121 ctatcttgtt tgtgtaaatc ttgaatcatt cgttcaattt tattaccata ttcgattgat
      181 tgatcatatt catacattaa ttccggcata atacgtaatc gcattctaga acctaattca
      241 gacttaatga agccttttgc tttatcaagt gctttaaatg tattttctac ttctttatcg
      301 ttacctaata cagttaaaaa tactttagcc tgcgataaat catttgttaa aacaacatct
      361 gtaattgtaa taaaaccaac tcgaggatct ttgactttat tgttgatgat atccattaat
      421 tccttcttca tttgttcacc aacacgctct gctctcatac tgctcattct tgtcacctct
      481 ttatctttaa gttcaattgt tatttgcatt ttttagtgta acaatgctta aacaattatt
      541 ttagctcaca cgttatttta ataaattaag cctataaaat caaataaagt ttgccgtaag
      601 ataggcattt caacagaaaa ttttccaata atagtcgaca ttatattttg tcattaaaaa
      661 tcattctagg ccaccacaaa attttataca aaatcatgtc catataacgt aacttttaat
      721 aggtaatcta ttctaataaa aaaccaaaag ctagtatcaa atatgctccc tataaagtag
      781 acacttaaaa agtgcaatat ttatagggag catatcaatc ttaactatac ttttagcttg
      841 taatttattt aattaacgct taatttcaac catttcaaat gcttcgataa catcgccttc
      901 tttaaggtca ttgtagtttt caattgtaat accacattca taaccttttg caacttcctt
      961 agcatcatct ttgaaacgtt taagtgtatc taattcgcct tcatattgaa caataccatc
     1021 acgaataata cgtacaccag catttcgcgt aattttacct tcagtaacat aacatccagc
     1081 aatagtacca actttagaaa ctttgaatgt ttgacgaact tcagcttgtc cgataacttg
     1141 ttcttcaaat tctggatcaa gtaaaccttt catcgctgat tcaatttctt cgataacatt
     1201 ataaataact ctgtgtaaac gcatatcaac attttcagct tctgcagcac gttttgcacc
     1261 actgtctgga cgaacattga aaccaatgat aataccattt gaggcattag caagtgtcac
     1321 gtctgactca ttaattgcac caaccgctgt atgaatgata cgaacattta cgccttcaac
     1381 atcaattttc attaatgatg cagctaaagc ttcaacagaa ccttgaacat cacctttaat
     1441 aataacgttt aaatctttca tttcaccttg tttcatttgt tcaaacaggt tatctaatga
     1501 aacattttta ctttcttgac gttgttgtat aatgctagct tcgtgtcttg attcaccaat
     1561 acgacgagct tgtttttcat cactaaatac aacaaagcga tccccagctt gtggcacatc
     1621 attaatacct gtaatttcaa caggcgttga tggaccagcc gttttgattc tttgacctaa
     1681 gtcattaacc attgcacgga tacggccgta agtattacca actacaatcg catcaccaac
     1741 atttaatgta ccgttttgta ctaataatga tgcagaagga ccacgtgatt tatctaattc
     1801 agcttcgata actgtaccaa cagcacggtt tttaggattt gctttaagtt cttgaacttc
     1861 tgcaactaat cctatcattt ctaataaatc gtcgatacca tcaccactta atgcagaaag
     1921 tggaacgaaa attgtttcgc cgccccaatc ttcaggaatt aaaccatatt cagttaattc
     1981 ttgcataact cgatcaggat ttgaagttgg tttatcaatt ttatttactg caacaataat
     2041 tggtacttct gcttctttag catggttaat tgcttcaatt gtttgtggca taacaccatc
     2101 gtcagctgct actactaaaa tagtaatatc tgttacttgt gcaccacgcg cacgcatcgt
     2161 tgtaaatgca gcatgtcccg gtgtatctaa gaaagtgatt tttttgccat cgttttcaat
     2221 ttgatatgca ccaatatgtt gagtgattcc gcctgcttca cctgctgtaa cttttgtatg
     2281 acgaattgaa tctaataaag tcgttttacc atggtcaaca tgtcccataa ttgttacaac
     2341 tgctggtctc tcaattgcct ctggatcatc tttttcgtct tcgaaataga ttgataagtc
     2401 ttcttcatta atcacaactt cttcttcaac ctcaacgcca taatcatcgg caattaattc
     2461 gattgtttct tgatttaatg attgattgat attagcaaca ataccaagta agaataattt
     2521 tttgataatt tctgatgatt caacatttaa tttatccgca aattcgccta ctgtaatacc
     2581 ttcttgatat gtcacttttg atggtatttc ttttggagca gctggttgat tttgtggttt
     2641 attattcttg ttatttttct tattatttct attattcttt ttattgcctt tgttgccttt
     2701 attattttgt tgttgattct ttttgttatc tttttgttgc cctttatttt ggttttgatt
     2761 gtttgatttt tggtgattat tttgagtgct ttgtttattg tcgttctttt gttctttttt
     2821 gaacttttta tctaatgctt taatttggtc atcttccaaa gcttgcatat gatttgaaac
     2881 ctcaatattc atgcttttta actcatctat aatctcttta ctctttagat ttaattcttt
     2941 cgcatattcg taaattcttt gtttactcat ataatcactc cttatgatat tcatctatca
     3001 ttgatagcaa ctttttagca aagcctgggt cagtaatccc tacattaaca cgctcacctt
     3061 ttccaagtgc tattcccaat tcatttcgat ttccaaactt tctgaatgga actttgtaac
     3121 tcttacattt atctgtaatt aatttagctg tattatcaga cgcatcattt gcaacaataa
     3181 cgagcttcaa atttcctttt ttaatctcat taacaatgac tgattcacct gtttttactt
     3241 taccagctct cattgctaat cctaaaaagt ttaatatttg atctatactc
//
LOCUS       DAXWFK010000204         3279 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.205, whole genome shotgun sequence.
ACCESSION   DAXWFK010000204 DAXWFK010000000
VERSION     DAXWFK010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.205"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            54..1436
                     /locus_tag="ACGEXS_002437"
     CDS             54..1436
                     /locus_tag="ACGEXS_002437"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFF8013419.1"
                     /translation="MKVSKFGGSSVSNATQIKKVLNIVNSDPERKIVIVSAPGKRHDN
                     DIKTTDLLIRLYEKVINHLDYHDKKREIIQRYDDIVKELQMDESILRTIDVTLEHYIN
                     QLKNEPKRLLDALLSCGEDFNAQLIALYNNSQGIPTKYISPKEAGIFVTDVPQEAQIL
                     DSAYSEIKKLRDYDEKLIIPGFFGVSHEGYIVTFPRGGSDITGAIISSGVRATIYENF
                     TDVSGIYKANPNIIKDPELIEEITYREMRELSYAGFSVFHDEALQPLYKDRIPVVIKN
                     TNRPQDKGTFIVHDREINAKNVISGISCDKDFTVINIKKYLMNRQVGFTRKILGVLED
                     NNISFDHMPSGIDTISIVMRSKQIQNKETKVLNEIRQKCDVDELSIEHDLAILMIVGE
                     GMNKVIGTANKITHALAESKINLKMINQGASEISMMFGIHVDDAEKAVLSTYEFCYHG
                     VALKNLCDKN"
     gene            complement(1490..2065)
                     /locus_tag="ACGEXS_002438"
     CDS             complement(1490..2065)
                     /locus_tag="ACGEXS_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013420.1"
                     /translation="MNAKEQLVDNLMKTSSQLFKFHGEVAMQLFLNDEFKLPSIVEIC
                     VERKRLSDIVKVIPQSYALLYIDKQDQAIAKEDLSLSKIAKVYVQYDDTTIMSIFVYD
                     VVNDEWIFRLDPNIRIPKSNIYFHSLNWDVDYIKPEIVLMYDLMQHHQYHHYSNYKRV
                     IDALSYYQFFILKFVVGEQRIKDAIQRTINN"
     gene            2396..3250
                     /locus_tag="ACGEXS_002439"
     CDS             2396..3250
                     /locus_tag="ACGEXS_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013421.1"
                     /translation="MSFYVVLIIIIVALIGILVLNQRYSNSKIDTEVYARKQLIKKNK
                     ALSAENAELRSQMLSSNNDVGHHAYKNAKRELRKILDSYLENGKLKYYDIIVTSNLAT
                     KHPFFEYARSFDFIIVSDIGLINVDVKSWGEKTFYHFDVPDEHDTEISNSNIEKVVGH
                     YISQQYHDQFNSSRKSIYTFTETVQPNRVIYDFYDYDPYQLAANNAKALKDHIEQNFN
                     FKVQSTGVIYFSDGTVNIIQGSEERDKYVDTVSTKSSLRRIISEAIELSKHPLNKEQV
                     DQITAIFK"
BASE COUNT         1209 a          472 c          514 g         1084 t
ORIGIN      
        1 acttacaaaa ctgaatacaa ttttctgatt ttttagaaag gttggcatgc actatgaaag
       61 tttccaaatt cggtggtagc tccgtctcca atgctactca aattaaaaag gttttaaata
      121 ttgttaattc tgatccagag cgaaagattg ttatcgtttc tgctccaggt aaaagacatg
      181 ataatgatat taaaacaact gatttgttaa tcagattata tgaaaaggtc attaatcatc
      241 ttgattatca tgataaaaaa agagaaatta ttcagcgtta tgatgatatt gtaaaagaat
      301 tgcaaatgga tgaaagtatt ttacggacga tagatgtgac tttggaacat tatattaatc
      361 aattaaaaaa tgaaccaaag agactattag atgcattact ttcttgtggt gaagatttta
      421 atgcgcaatt gatagcttta tataataata gtcaaggtat accaacaaaa tatatatcac
      481 cgaaggaagc gggtattttt gttactgacg tcccacaaga agcacaaata ttagatagtg
      541 cttactctga aattaaaaaa ttaagagatt atgatgaaaa actaattatc ccaggatttt
      601 ttggcgtatc tcatgaagga tatatagtta catttccacg tggcggatca gatataactg
      661 gcgccatcat atcaagtggt gttagagcca caatttatga aaatttcacc gatgtgtctg
      721 gtatttataa agctaatcca aatatcatca aagatcctga attaatcgaa gaaatcactt
      781 atcgagaaat gcgtgaatta tcttatgctg gttttagtgt tttccatgat gaagccttac
      841 aaccacttta taaagatcgc atccctgtgg tgattaaaaa tacgaaccgc cctcaagata
      901 agggtacatt tatagtgcat gatagagaaa taaatgcgaa aaatgtgatt agcggtataa
      961 gttgtgataa agattttacc gttattaata tcaaaaagta tttaatgaat agacaagtcg
     1021 gctttactag aaagatatta ggtgtcttag aggataataa tatttcattt gatcatatgc
     1081 cttcaggtat tgatactatc agtatcgtta tgcgttcaaa acaaattcaa aataaagaaa
     1141 caaaagtatt aaatgaaatt cgtcaaaaat gtgatgttga tgagctaagt attgagcatg
     1201 atttagcaat attaatgatt gtcggcgaag gtatgaataa agtcatcggt actgcaaata
     1261 aaattacaca tgccttagct gaatcaaaaa ttaacttaaa aatgattaac caaggtgctt
     1321 cggaaatctc gatgatgttt ggaattcatg tagatgatgc tgaaaaagct gtattatcta
     1381 catatgagtt ttgctatcac ggggttgcat taaaaaactt gtgtgataaa aactaatata
     1441 aaagcaattc gtaacacatg tattcaactg cgttatgaat tgctttttct taattattta
     1501 ttgttctctg gattgcatcc ttaatacgtt gctcacctac tacaaatttt aaaataaaaa
     1561 attgatagta gcttaatgca tctatgactc gtttataatt ggaataatga tgatactgat
     1621 ggtgttgcat tagatcatac attagaacga tctccggttt aatataatcc acatcccaat
     1681 ttaaactatg gaagtatatg ttactcttag gtatacgtat attcggatcc aatctaaaaa
     1741 tccattcatc gtttactaca tcataaacga aaatactcat tattgttgta tcatcatatt
     1801 gcacataaac ttttgcaatt tttgaaagtg ataaatcttc tttagctatt gcttgatctt
     1861 gcttatctat gtatagtaac gcatatgatt gcggaataac tttcacaata tcacttaaac
     1921 gcttacgttc cacgcatatt tcaacaatag aaggtaattt aaattcatca tttaagaaaa
     1981 gctgcatggc aacttcaccg tgaaatttaa ataattgcga tgatgttttc attaaattgt
     2041 ccactaattg ttctttagca ttcatttcat tcactctcaa atacacctcc taagcaagcg
     2101 ctttcacaaa tatcattata cattttaaaa ttaatgaatt caatcttcaa aatactatga
     2161 gtgacctaat tctgtcattt aaatgaccgt tataacataa attcgacata aacttgaatt
     2221 cacgaatctt tcacgtcata ttcattgaac atcaatatct gtcagtttat aatttgatta
     2281 taacaatgat gaactaaaat caactaataa aaatgtggga aattgaataa caattaatct
     2341 ttattaatta ttattgtata caaagttaaa gaagtcaaaa aggattggtg aaaaaatgtc
     2401 tttttatgtt gtacttatca ttatcattgt agcattaatt ggtatattag ttttaaatca
     2461 aaggtatagt aatagtaaaa tagataccga agtttatgca agaaagcaac ttattaaaaa
     2521 gaacaaagca ttaagtgcag aaaatgctga attaagaagt caaatgctga gttctaataa
     2581 tgatgttggc caccatgcat acaaaaatgc taaaagagaa ttacgcaaga ttttagatag
     2641 ctatctcgaa aatggtaaat taaaatatta cgatattatc gttacaagca atttagctac
     2701 taaacatccg tttttcgagt atgcacgttc atttgatttt atcattgttt cagatatcgg
     2761 tttaataaac gttgatgtaa aaagctgggg tgaaaaaaca ttttatcact ttgatgtacc
     2821 tgatgagcat gatacagaaa taagcaatag taatattgaa aaagttgtcg gtcattacat
     2881 tagccagcaa tatcatgatc agtttaattc atcaagaaag tctatctata cttttacaga
     2941 aacagttcaa ccaaatcgtg ttatttatga tttttatgat tatgatccgt atcaattagc
     3001 agcaaataat gcgaaagcat taaaggatca tattgaacaa aactttaact ttaaagtcca
     3061 aagtaccggt gttatttact ttagtgatgg tacagttaat atcattcaag gttctgaaga
     3121 aagagataaa tatgttgata cagtttcaac aaaatcttcg ttacgacgta ttattagcga
     3181 agccatagag ctatctaaac accctttaaa taaagaacag gttgatcaaa tcacagctat
     3241 attcaagtaa aatcaaaggc ataataatcc attacgctc
//
LOCUS       DAXWFK010000205         3169 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.207, whole genome shotgun sequence.
ACCESSION   DAXWFK010000205 DAXWFK010000000
VERSION     DAXWFK010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.207"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(97..1233)
                     /locus_tag="ACGEXS_002440"
     CDS             complement(97..1233)
                     /locus_tag="ACGEXS_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFF8013422.1"
                     /translation="MTENKSFKESHPLDDFISDKELSNTTIQKEKLTIEQQKQVDTIS
                     KQINPLDNEGLLAFGSDLQKQISQFSHQMLDEVQSKDVGPIGDTLSDLMSKLKSVNPN
                     ELNTDKPSMLKRIFSRAKSSINEIFSRMQSVSAQVDRITIQLQKHQTHLTRDIELLDT
                     LYDKNKQYFDDLSLHIIAAQQKKLQLENEKLPQLQQQAQQSTNQMDIQQVADMQQFID
                     RLDKRIYDLQLSRQIALQTAPQIRMIQNVNQALAEKIQSSILTSIPLWKNQMAIALTL
                     MRQRNAVAAQRAVTDTTNDLLTANAEMLKQNAIETATENERGIVDLDTLKRTQRNIIE
                     TIEETLIIQQHGREERQLAEKELQQLEQDLKSHLVNIKGPNKQS"
     gene            complement(1265..1894)
                     /locus_tag="ACGEXS_002441"
     CDS             complement(1265..1894)
                     /locus_tag="ACGEXS_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFF8013423.1"
                     /translation="MTVRYNISHIFGVLVGIPVAFLTSIFGMIALDVSFLIDMSIGIV
                     GFLMTYLPIQKLTSRKYLNEIGLTRKDYRYIRNQLNHTHQKLRGILKTYVNIRSIKDF
                     RQINDIYQISRSIYTTVRQRPASFYKVEGFFYSHIDNALNLVDAYTRLAKMPKKSINE
                     QQKLEQTRITLDEVKRTLIADLKRLNEDDYERLDIEMELNKLHQKHHQD"
     gene            complement(1913..2182)
                     /locus_tag="ACGEXS_002442"
     CDS             complement(1913..2182)
                     /locus_tag="ACGEXS_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFF8013424.1"
                     /translation="MRHIHLQVFGRVQGVGFRYFTQRIAMNYNIVGTVQNVDDYVEIY
                     AQGDDADIERFIQGVIEGASPASNVTSHQLEELELNQKLSDFRSI"
     gene            2345..2653
                     /gene="msaA"
                     /locus_tag="ACGEXS_002443"
     CDS             2345..2653
                     /gene="msaA"
                     /locus_tag="ACGEXS_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein MsaA"
                     /protein_id="HFF8013425.1"
                     /translation="MWTVTKIRADYEGWWLFSDWPENIVEKYQYQDFDDMFKHYQQLI
                     NQCKVQFDNYVTGKYNIYAFYNNCDMNYCEDCEEDLQIFYSFIVLQNNEVYYKLPIID
                     "
     gene            2824..3024
                     /gene="cspA"
                     /locus_tag="ACGEXS_002444"
                     /gene_synonym="msaB"
     CDS             2824..3024
                     /gene="cspA"
                     /locus_tag="ACGEXS_002444"
                     /gene_synonym="msaB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspA"
                     /protein_id="HFF8013426.1"
                     /translation="MKQGTVKWFNAEKGFGFIEVEGENDVFVHFSAINQDGYKSLEEG
                     QAVEFEVVEGDRGPQAANVVKL"
BASE COUNT          978 a          481 c          503 g         1207 t
ORIGIN      
        1 atcaaataaa taatagaaat cagctttttt acattgccta agaactttaa ggaacttaat
       61 gtcccaagct cttttttgtt atatctaatt cgtaatttat gattgtttat tcggtccttt
      121 gatgttcact aaatgtgact ttaaatcttg ttctaattgt tgtaattctt tttcagctaa
      181 ttgtcgttct tcgcgaccgt gttgttgaat aattaatgtt tcttcaattg tctcaataat
      241 gttacgctgt gtacgtttca atgtatcaag atcaacaatg ccacgctcat tttctgttgc
      301 agtttcaatc gcattttgtt tcaacatttc agcatttgct gttaataaat cattagttgt
      361 atcagtgaca gctcgttgtg cagcaactgc attacgctgt ctcattaatg taagcgcaat
      421 ggccatttga tttttccata gtggaatact tgtcaaaatt gaactttgta tcttctcggc
      481 aagtgcttga ttaacatttt gaatcatacg aatttgtggc gcagtttgca aagctatttg
      541 tcttgaaagc tgtaagtcat atatgcgttt atctagtcta tctataaatt gctgcatatc
      601 tgcaacttgt tgaatatcca tttgattagt ggattgctgc gcttgctgtt gcaattgtgg
      661 tagcttttca ttttctaatt gcaacttttt ttgctgtgca gcaatgatat gcaatgataa
      721 gtcatcaaag tattgtttgt ttttatcata tagcgtatct aataattcaa tatctcttgt
      781 taaatgtgtt tgatgtttct gcagttgaat cgttatgcga tcgacttgag cactaactga
      841 ttgcattctt gaaaagattt cattgataga cgactttgct ctgctaaaaa ttctttttaa
      901 catagatggt ttatcagtat ttaactcatt tggattaact gactttagtt ttgacattag
      961 atctgacaaa gtatctccaa taggaccaac atctttactt tgtacttcat ccaacatttg
     1021 atgtgaaaat tgagatattt gtttctgtaa atcagaacca aacgctaata aaccttcatt
     1081 gtctaaaggg ttaatttgtt tactgattgt gtctacctgt ttttgttgtt caattgttaa
     1141 cttttctttt tgaatagtag tattcgataa ttctttatcg cttataaaat catctagtgg
     1201 atggctttct ttgaaacttt tattttcagt catcaatcat ctctccaata gttgctattt
     1261 tacatcaatc ttgatgatgt ttttgatgta acttatttaa ttccatttca atatctaaac
     1321 gttcataatc atcttcgttg agacgcttta aatcagcgat taatgttcgt ttgacctcat
     1381 ccaaagtaat tcgtgtttgt tctaactttt gctgttcatt aattgatttt ttgggcattt
     1441 ttgctagacg tgtatatgca tcaaccaaat ttaaagcatt atcaatatga gaataaaaaa
     1501 agccttcaac tttataaaat gatgcaggtc tctgtctaac tgtcgtataa atagaacgtg
     1561 aaatttggta tatatcatta atctgcctaa aatctttaat tgatcttata ttgacatacg
     1621 tttttaaaat acctctaagt ttttggtgtg tatgatttaa ctgatttcga atatagcgat
     1681 agtcttttct agtcaaacca atttcgttta aatatttgcg tgaagtgagt ttttgtatcg
     1741 gtaggtatgt cattaaaaag ccaacaatac caatagacat atcaattaaa aaagatacat
     1801 caagtgcaat catcccaaat atgcttgtta aaaacgctac aggaattccc actaacaccc
     1861 caaatatatg agaaatatta tatctcactg tcatcttcct ttatttagca ttttatattg
     1921 atcgaaaatc cgataatttt tgatttagtt ctaactcttc aagttgatgg cttgttacat
     1981 ttgatgctgg tgaggcacct tcaattacac cttgaataaa tctctctata tctgcgtcat
     2041 ccccttgtgc atatatctct acatagtcat ctacattttg aacagtaccg acaatgttat
     2101 agttcattgc aatgcgttgt gtaaaatatc taaatccgac gccttgaacg cgtccgaata
     2161 cttgtaaatg tatatgtctc atttttacca cctcataatg ttattatacg tagttttact
     2221 taaaaaaact aataattact atagttacta ctttgtttgt ttcaagtcgt caaacttgat
     2281 tttcagagga taaaggtata aaaataagta tagagttttt gaagtatgga aggggtcttt
     2341 aataatgtgg acagttacca aaattagagc cgattatgag ggatggtggt tattcagtga
     2401 ctggccagaa aacattgttg aaaaatatca atatcaagat tttgatgaca tgtttaagca
     2461 ctatcaacaa ttgattaatc aatgtaaagt tcagttcgat aactatgtca caggcaaata
     2521 taatatttat gcattttata ataattgtga catgaactat tgcgaagatt gtgaagagga
     2581 tttacaaata ttttatagtt tcattgtttt acaaaataat gaagtatatt ataaactacc
     2641 aataattgat taaatgaata ttttttatta ttgtattgca atttggtaat aactgcacta
     2701 taatacaagt taagcagatg attattccat attgcaaaga atatttgagt gaaatttgca
     2761 tgtatttttt gtaatatgcg aataagcata ttgaatgaat tttagtcttg gaggtttcag
     2821 attatgaaac aaggtacagt taaatggttt aacgctgaaa aaggattcgg ctttatcgaa
     2881 gttgaaggag aaaatgacgt attcgtacat ttttcagcaa ttaaccaaga tggttacaaa
     2941 tcattagaag aaggtcaagc tgttgagttt gaagtagttg aaggcgaccg cggtccacaa
     3001 gctgcaaacg ttgttaaact ataattctta gatttgaatc attgatttta actaacacct
     3061 taccaagaag taaggtgttt ttatatgctt attacaaata cttaacgcga tgagtttctc
     3121 aaccaaattg agaactcttt ttttatatca ccaacattgt ttgcaatag
//
LOCUS       DAXWFK010000206         3167 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.208, whole genome shotgun sequence.
ACCESSION   DAXWFK010000206 DAXWFK010000000
VERSION     DAXWFK010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.208"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            114..1082
                     /gene="pfkA"
                     /locus_tag="ACGEXS_002445"
     CDS             114..1082
                     /gene="pfkA"
                     /locus_tag="ACGEXS_002445"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002462395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFF8013427.1"
                     /translation="MKKIAVLTSGGDSPGMNAAVRAVVRTAIYNEIEVYGVYHGYQGL
                     LNDDIHKLELGSVGDTIQRGGTFLYSARCPEFKEQEVRKVAIENLRKRGIEGLVVIGG
                     DGSYRGAQRISEECKEIQTIGIPGTIDNDINGTDFTIGFDTALNTIIGLVDKIRDTAS
                     SHARTFIIEAMGRDCGDLALWAGLSVGAETIVVPEVKTDIKEIADKIEQGIKRGKKHS
                     IVLVAEGCMTAQDCQKELSQYINVDNRVSVLGHVQRGGSPTGADRVLASRLGGYAVDL
                     LMQGETAKGVGIKNNKIVATSFDEIFDGKDHKFDYSLYELANKLSI"
     gene            1104..2861
                     /gene="pyk"
                     /locus_tag="ACGEXS_002446"
     CDS             1104..2861
                     /gene="pyk"
                     /locus_tag="ACGEXS_002446"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFF8013428.1"
                     /translation="MRKTKIVCTIGPASESEEMIEKLINAGMNVARLNFSHGSHEEHK
                     GRIDTIRKVAKRLDKIVAILLDTKGPEIRTHNMKDGIIELERGNEVIVSMNEVEGTPE
                     KFSVTYENLINDVQVGSYILLDDGLIELQVKDIDHAKKEVKCDILNSGELKNKKGVNL
                     PGVRVSLPGITEKDAEDIRFGIKENVDFIAASFVRRPSDVLEIREILEEQKANISVFP
                     KIENQEGIDNIAEILEVSDGLMVARGDMGVEIPPEKVPMVQKDLIRQCNKLGKPVITA
                     TQMLDSMQRNPRATRAEASDVANAIYDGTDAVMLSGETAAGLYPEEAVKTMRNIAVSA
                     EAAQDYKKLLSDRTKLVETSLVNAIGISVAHTALNLNVKAIVAATESGSTARTISKYR
                     PHSDIIAVTPSEETARQCSIVWGVQPVVKKGRKSTDALLNNAVATAVETGRVSNGDLI
                     IITAGVPTGETGTTNMMKIHLVGDEIANGQGIGRGSVVGTTLVAETVKDLEGKDLSDK
                     VIVTNSIDETFVPYVEKALGLITEENGITSPSAIVGLEKGIPTVVGVEKAVKNISNNM
                     LVTIDAAQGKIFEGYANVL"
BASE COUNT         1116 a          423 c          659 g          969 t
ORIGIN      
        1 ttcaatattt aaaacgttta catcaattca atacattagt tttgatggaa tgacatatca
       61 atttgtggta atttagagtt aaagataaat cagttataga aaggtatgtc gtcatgaaga
      121 aaattgcagt tttaactagt ggtggagatt cacctggaat gaatgctgcc gtaagagcag
      181 ttgttcgtac agcaatttac aatgaaattg aagtttatgg tgtgtatcat ggttaccaag
      241 gattgttaaa tgatgatatt cataaacttg aattaggatc agttggggat acgattcagc
      301 gtggaggtac attcttgtat tcagcaagat gtccagagtt taaggagcaa gaagtacgta
      361 aagttgcaat cgaaaactta cgtaaaagag ggattgaggg ccttgtagtt attggtggtg
      421 acggtagtta tcgcggtgca caacgcatca gtgaggaatg taaagaaatt caaactatcg
      481 gtattcctgg tacgattgac aatgatatca atggtactga ttttacaatt ggatttgaca
      541 cagcattaaa tacgattatt ggcttagtcg acaaaattag agatactgcg tcaagtcacg
      601 cacgaacatt tatcattgaa gcaatgggcc gtgattgtgg agatctagca ttatgggctg
      661 gattatcagt tggtgctgag acaattgtag ttccagaagt gaaaacagat attaaagaaa
      721 tagctgataa aattgaacaa ggtattaaac gtggtaagaa acactcaatc gttcttgtag
      781 cagaaggttg tatgactgcg caagattgtc aaaaagaatt atcacaatac atcaatgttg
      841 ataatagagt gtctgtgtta ggtcacgttc aacgtggtgg tagcccaaca ggtgcggata
      901 gagttttagc atcacgttta ggtggatatg cggtagactt attaatgcaa ggtgaaacag
      961 ctaagggtgt tggaattaag aacaataaaa ttgtagcaac atcttttgat gaaatttttg
     1021 atggtaaaga tcataaattt gattatagtc tatatgaact tgctaacaag ttatctatat
     1081 aagatttcag gaggaattat aaaatgagaa aaactaaaat tgtatgtaca attggaccag
     1141 cttcagaatc agaagaaatg attgagaaat taatcaatgc tggtatgaac gttgcacgat
     1201 taaacttttc acatggtagt catgaagagc ataaaggtag aattgataca attcgtaaag
     1261 tagctaaaag attagacaaa attgtagcaa ttttattaga tacaaaaggt ccagaaattc
     1321 gtacgcataa tatgaaagac ggtatcattg aacttgaacg tggcaacgaa gttattgtta
     1381 gcatgaatga agttgaagga acacctgaaa agttctcagt aacatatgaa aacttaatta
     1441 acgatgttca agtaggttca tacattttac ttgatgatgg cttaattgaa ttacaagtta
     1501 aagatattga ccatgctaaa aaagaagtta aatgtgatat tttaaactct ggtgagctta
     1561 aaaacaaaaa aggtgttaac ttacctggcg taagagtaag tttacctggt attacagaaa
     1621 aagatgctga agatatccgt ttcggtatta aagaaaatgt tgacttcatt gcagcaagtt
     1681 tcgtacgtcg tcctagtgat gttttagaaa ttcgtgaaat tttagaagaa caaaaagcta
     1741 acatttcagt attccctaaa attgaaaacc aagaaggtat tgataatatt gcggaaattc
     1801 ttgaagtgtc tgatggttta atggttgcac gtggtgacat gggtgttgaa attccacctg
     1861 aaaaagtacc aatggttcaa aaagatttaa tcagacaatg taacaaatta ggtaaaccag
     1921 ttattacagc tacacaaatg ttagattcta tgcaacgtaa cccacgtgct acacgtgcag
     1981 aagctagtga cgttgccaac gcaatctatg atggtacaga tgcagtaatg ttatctggtg
     2041 aaactgctgc tggtttatat cctgaagaag ctgttaaaac aatgagaaat attgctgtat
     2101 cagctgaagc agcccaagat tacaaaaagt tattgtcaga tcgtactaaa ttagttgaaa
     2161 cttcattagt gaatgctatc ggtatttcgg ttgcacatac agctttaaac ttaaatgtta
     2221 aagcaattgt agctgctact gaaagtggtt caacggcacg tactatctcc aaatatcgtc
     2281 cacattcaga cattattgcg gtgactccaa gtgaagaaac tgcacgtcaa tgttcaattg
     2341 tttggggagt tcaacctgta gttaaaaaag gacgtaagag tacagatgca ttgttaaaca
     2401 atgcagttgc aacagctgtt gaaactggta gagtatctaa tggtgattta atcattatta
     2461 ctgctggtgt accaactggt gaaactggaa ctactaatat gatgaaaatc cacctagttg
     2521 gtgacgaaat tgctaatggt caaggtattg gacgtggatc agttgttggt actacgttag
     2581 ttgctgaaac tgttaaagat ttagaaggta aagatttatc tgacaaagtt atcgttacta
     2641 actcaatcga tgaaacgttt gtaccttatg tagaaaaagc tttaggctta attacagaag
     2701 aaaatggtat tacatcacca agtgcaattg ttggtttaga aaaaggtatt ccaacagttg
     2761 taggtgtaga aaaagctgtt aaaaacataa gcaataacat gttagttacg attgatgctg
     2821 ctcaaggtaa aatctttgaa ggatatgcaa acgtactata atttataaaa aaacgtcttt
     2881 ccatttatca acaatggaaa ggcgtttttt ggttcatctg gtattttatg acgtaattaa
     2941 taggttattt gataatgata gtgtatgaat ggcaatctat ataaatgttt atatctttta
     3001 tacatgtaca ttatcacctt caaactttca ctcatattac tttggaaatt tattataaaa
     3061 tagaagtatg gatgtatttc tgaaatgata cattattaaa tagatgagaa agtaaaagtt
     3121 ttgagccaag tacgcaattt aatattataa gttgcatata aaacagg
//
LOCUS       DAXWFK010000207         3142 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.209, whole genome shotgun sequence.
ACCESSION   DAXWFK010000207 DAXWFK010000000
VERSION     DAXWFK010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.209"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(105..941)
                     /locus_tag="ACGEXS_002447"
     CDS             complement(105..941)
                     /locus_tag="ACGEXS_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HFF8013429.1"
                     /translation="MDLASKYFNGVNWRYIDHSSGLEPMQSFAFDDTFCESVGKDISD
                     NVVRTWIHQHTVILGIHDSRLPFLKDGIDYLTNEIGYNAIVRNSGGLGVVLDQGVLNI
                     SLMFKGQTETTIDEAFTVMYLLISKMFENENVDIDTMEIEHSYCPGKFDLSIDGKKFA
                     GISQRRVRGGIAVQIYLCVEGSGSERALMMQTFYEHALKGEVTKFKYPEIEPSCMASL
                     ETLLNKTITVQDVMFLLLYAIKDLGGVLNMTPITQEEWQRYDTYFDKMIERNKKMIDQ
                     MQ"
     gene            complement(944..1930)
                     /gene="pta"
                     /locus_tag="ACGEXS_002448"
     CDS             complement(944..1930)
                     /gene="pta"
                     /locus_tag="ACGEXS_002448"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFF8013430.1"
                     /translation="MADLLNVLKDKLSGKNVKIVLPEGEDERVLTAATQLQATDYVTP
                     IVLGDETKVQSLAQKLDLDISNIELINPATSELKAELVQSFVERRKGKATEEQAQELL
                     NNVNYFGTMLVYAGKADGLVSGAAHSTGDTVRPALQIIKTKPGVSRTSGIFFMIKGDE
                     QYIFGDCAINPELDSQGLAEIAVESAKSALSFGMDPKVAMLSFSTKGSAKSDDVTKVQ
                     EAVKLAQQKAEEEKLEAIIDGEFQFDAAIVPGVAEKKAPGAKLQGDANVFVFPSLEAG
                     NIGYKIAQRLGGYDAVGPVLQGLNSPVNDLSRGCSIEDVYNLSIITAAQALQ"
     gene            2098..2850
                     /gene="hemQ"
                     /locus_tag="ACGEXS_002449"
     CDS             2098..2850
                     /gene="hemQ"
                     /locus_tag="ACGEXS_002449"
                     /EC_number="1.3.98.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006785 - heme B biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="HFF8013431.1"
                     /translation="MSQAAETLDGWYSLHLFYAVDWASLRIVPKDERDALVTEFQSFL
                     ENTATVRSSKSGDQAIYNITGQKADLLLWFLRPEMKSLNHIENEFNKLRIADFLIPTY
                     SYVSVIELSNYLAGKSDEDPYENPHIKARLYPELPHSDYICFYPMNKRRNETYNWYML
                     TMEERQKLMYDHGMIGRKYAGKIKQFITGSVGFDDFEWGVTLFSDDVLQFKKIVYEMR
                     FDETTARYGEFGSFFVGHIINTNEFDQFFAIS"
BASE COUNT         1003 a          581 c          477 g         1081 t
ORIGIN      
        1 gttcaattta aatattaggt caataacata tttatgcttt ttatggatac tttcaaaaat
       61 aacagcccca aacgataact tgaaaggggc tgttaaatat ttaactattg catttgatct
      121 atcattttct tgtttctttc aatcatttta tcaaaatacg tatcgtatct ttgccattct
      181 tcttgagtaa ttggcgtcat atttaataca ccgccaagat ctttgattgc atataatagt
      241 aaaaacatta catcttgaac agtaatcgtt ttgttaagca atgtctctaa tgaggccata
      301 caagatggtt caatttcagg atatttaaat ttagtcactt caccttttaa agcatgttca
      361 taaaatgttt gcatcatcaa tgcacgttct gaaccagagc cttcaacaca aagataaatt
      421 tgtacagcaa taccgcctct aactcttcgt tgcgatatgc ctgcaaattt cttaccatcg
      481 atacttaagt caaattttcc tgggcaataa gaatgttcaa tttccatcgt atcaatatca
      541 acattctcat tttcgaacat tttgctaatt aagaggtaca tcacagtaaa cgcttcatca
      601 atcgttgttt ctgtttgtcc tttgaacatc agcgatatat ttaatacacc ttgatctaga
      661 acgacaccta agccaccaga atttctaaca atggcattat aaccaatctc attcgttaaa
      721 taatcaatgc catcttttaa aaacggcaat cttgaatcat gaataccaag aataacagta
      781 tgttgatgaa tccaagtacg cacaacatta tctgatatat ctttgcccac actttcgcaa
      841 aatgtatcat cgaatgcgaa agattgcata ggttctaatc cagaagaatg atcgatatat
      901 cgccagttga cgccattaaa atatttactc gctaaatcca tcgttattgt aaggcttgcg
      961 ctgctgtaat aattgaaaga ttgtatacat cttcaattga gcagccacgt gataagtcat
     1021 ttactggaga atttaaacct tgtaatactg gaccaactgc atcatatcca cctaaacgtt
     1081 gtgcaatttt gtaaccaata ttaccagctt ctaaacttgg gaatacaaag acatttgcat
     1141 caccttgtaa tttagcacct ggcgcttttt tctcagcaac acctggtaca atcgcagcat
     1201 caaattggaa ttcgccatca atgattgctt ctaatttttc ttcttcagct ttttgttgtg
     1261 ctaatttgac agcttcttga acttttgtca cgtcgtctga tttagcagac ccttttgttg
     1321 aaaagcttaa cattgcaact tttggatcca tgccaaagct taatgctgat tttgcacttt
     1381 ctactgcaat ttctgcaagt ccttgtgaat caagttctgg attgattgca caatcaccaa
     1441 agatgtattg ttcatcacct ttaatcataa agaagatacc tgatgttctt gatacacctg
     1501 gtttcgtttt gatgatttgt aaagctggac gcacagtgtc gcctgttgaa tgtgctgcac
     1561 cactaactaa accatctgct ttaccagcat aaacaagcat tgtaccgaag tagttcacat
     1621 tgtttaataa ttcttgtgct tgttcttcag tcgctttacc tttacgtcgt tcaacaaatg
     1681 attgaactaa ttcagctttc aattcacttg tcgcaggatt aattaattca atattagaaa
     1741 tatcaagatc aagtttttgc gctaaagatt gaaccttagt ctcatcacct aacacgattg
     1801 gtgtaacata atctgttgct tgtaattgtg ttgcagctgt tagaacacgt tcgtcctctc
     1861 cttcaggtaa tacgatttta acgtttttac cagaaagttt gtcttttaat acatttaata
     1921 aatcagccat aatgtcctcc tgtaatataa atcttattaa tcattcacgg tataattata
     1981 cgccatttta tttcataatg ccacgtcacc aatttgtaat cataatttta tcgttacccc
     2041 tttgctttat gataaaattt ataaagaact gatgattttt gaaaaggagc gataaacatg
     2101 agtcaagcag ccgaaacatt agatggttgg tatagtctac atttatttta tgcagttgat
     2161 tgggcatcat tacgtatagt tccaaaggac gaacgcgatg cacttgtcac tgaatttcaa
     2221 tcatttttag aaaatacagc aactgtaaga tcatcaaaat ctggtgatca agctatttat
     2281 aatataactg gtcaaaaagc agatttgtta ttatggttct tacgtcctga aatgaagtct
     2341 ttaaatcata ttgaaaatga atttaacaaa ttgcgcattg ctgacttcct aatccctaca
     2401 tattcatatg tatcagtcat tgaattgagc aattatttag ctggtaaatc tgatgaagat
     2461 ccttatgaga accctcatat caaagcaaga ttatacccag aattaccaca ttctgattat
     2521 atttgtttct atccaatgaa caaacgtcgt aatgaaactt ataactggta catgttaact
     2581 atggaagaac gccaaaaatt aatgtatgac catggtatga ttggtagaaa atatgctggc
     2641 aaaatcaaac aatttattac tggttctgta gggtttgatg atttcgaatg gggcgtaaca
     2701 ttgttctcag atgacgtatt acaattcaaa aaaattgtat acgaaatgcg ctttgatgaa
     2761 acaacagcac gatacggtga attcggtagt ttctttgtag gacatattat taacacaaac
     2821 gaattcgatc aattctttgc gatttcttaa tacattggta cgtttataaa ttaataaaaa
     2881 aattccaagc ttatcggttt aagcttggaa tttttcgttt atcttcagta tattcccgta
     2941 tacataagac gtgatttggt aaatagttga aatctgtatg tttaaactta tatatatgtg
     3001 ctaatgtatt atcaataaca aagtacactt tgctcatagc aaagtgtacc cgagtagtct
     3061 tccttgggag aactttaact actatcacta catataaacg ttaacctcaa tagaaattat
     3121 acagtcgcta ctctatacaa tt
//
LOCUS       DAXWFK010000208         3117 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.210, whole genome shotgun sequence.
ACCESSION   DAXWFK010000208 DAXWFK010000000
VERSION     DAXWFK010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.210"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            66..1172
                     /gene="rpoD"
                     /locus_tag="ACGEXS_002450"
     CDS             66..1172
                     /gene="rpoD"
                     /locus_tag="ACGEXS_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFF8013432.1"
                     /translation="MSDNTVKIKKQTIDPTLTLEDVKKQLIEKGKKEGHLSHEEIAEK
                     LQNFDIDSDQMDDFFDQLNDNDISLVNEKDSSDTDEKLNPSDLSAPPGVKINDPVRMY
                     LKEIGRVNLLSAQEEIELAKRIEQGDEVAKSRLAEANLRLVVSIAKRYVGRGMLFLDL
                     IQEGNMGLIKAVEKFDFNKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LIRVQRQLLQDLGRDPAPEEIGEEMDLPAEKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDQEAQSPSDHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVGK
                     VFGVTRERIRQIEAKALRKLRHPSRSKRLKDFMD"
     gene            1303..1980
                     /locus_tag="ACGEXS_002451"
     CDS             1303..1980
                     /locus_tag="ACGEXS_002451"
                     /EC_number="2.1.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFF8013433.1"
                     /translation="MISLNNRLTTVSRFLKQGTIADIGSDHAYLPIYAIQNHLCECGI
                     AGEVIQGPFQAAVKNVAANQLVDRIDVRLGDGLSVIQPEDVIDNITICGMGGPLIAKI
                     LKDGQDKLSQHPRLILQSNIQTENLRQTLQQLNYEIIDEIIMEEKGHIYEIVVAEYST
                     ELIELSSDELKFGPKLLNNKNEYFIKKWQRELEALYHIKSKLNTEQHHQRLAQINDEI
                     AVIERVL"
     gene            1983..3083
                     /locus_tag="ACGEXS_002452"
     CDS             1983..3083
                     /locus_tag="ACGEXS_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFF8013434.1"
                     /translation="MKIADLMTLLDHHVPFSTAESWDNVGLLIGDEDVEVTGVLTALD
                     CTLEVVNEAIEKGYNTIISHHPLIFKGVTSLKANGYGLIIRKLIQHDINLIAMHTNLD
                     VNPYGVNMMLAKAMGLKNISIINNQQDVYYKVQTYIPKDNVGPFKDKLSENGLAQEGN
                     YEYCFFESEGRGQFKPVGEANPTIGQIDKIEYVDEVKIEFMIDAYQKSRAEQLIKQYH
                     PYETPVFDFIEIKQTSLYGLGVMAEVDNQMTLEDFAADIKSKLNIPSVRFVGESNQKI
                     KRIAIIGGSGIGYEYQAVQQGADVFVTGDIKHHDALDAKIHGVNLIDINHYSEYVMKE
                     GLKALLMNWFNTEKINLDVEASTINTDPFQYI"
BASE COUNT         1174 a          399 c          593 g          951 t
ORIGIN      
        1 ttagtttaaa atagcaatat tgaataaatt tcgaatgttc atatttaaaa tcgggaggcc
       61 gtttcatgtc tgataacaca gttaaaatta aaaaacaaac aattgatccg acattaacat
      121 tagaagatgt taagaagcaa ttaattgaaa aaggtaaaaa agagggtcat ttaagtcatg
      181 aagaaattgc tgaaaaactt cagaattttg atatcgactc tgatcaaatg gatgatttct
      241 ttgatcaatt aaatgataat gatatttcac tagttaatga aaaagatagt tcagatactg
      301 acgagaaact gaatccaagt gatcttagtg cccctccagg tgttaaaata aatgacccag
      361 ttcgtatgta ccttaaagaa attgggcgtg ttaacttatt aagtgcacaa gaagaaatcg
      421 aattagccaa acgtattgaa caaggtgatg aagtagcaaa atcaagactt gcagaagcga
      481 acttacgttt agttgtaagt attgctaaaa gatacgtagg tcgtggtatg ttattccttg
      541 atttaatcca agaaggtaat atgggtctta ttaaagctgt tgaaaaattt gactttaaca
      601 aaggatttaa gttttcaaca tatgcaacat ggtggattag acaagcaatc actcgtgcaa
      661 ttgctgacca agcacgtacg attcgtatcc ctgtgcatat ggtagaaaca attaataaat
      721 taattcgtgt tcaacgtcaa ttattacagg acttaggtcg agatccagca ccagaagaaa
      781 ttggtgaaga aatggattta ccagcagaaa aagttcgtga aattttaaaa attgcgcaag
      841 aacctgtttc attagaaaca ccaattggtg aagaagatga tagtcattta ggagacttta
      901 ttgaggatca ggaagcacaa agtccttcag atcatgctgc ttatgaatta ttaaaagagc
      961 aattagaaga tgtgcttgat acattaactg atagagaaga aaatgtatta cgattaagat
     1021 ttggtcttga tgacggcaga acaagaacac ttgaagaagt tggtaaagtt ttcggtgtta
     1081 cacgtgaacg tattcgacaa attgaagcaa aagcacttag aaaattaaga catccaagtc
     1141 gtagtaaacg tttgaaagac tttatggatt aatttatatc aattttaatg attgatacca
     1201 agacatgaat aagcgcttat ttatttttag attaagattt aacttgagtg aggttggtac
     1261 attgaataat gttcaacctc ttttttcata aaggagaata aaatgatttc gttaaataac
     1321 cgattaacga cggtaagtcg atttttaaaa caaggtacaa tcgcggatat tggctcagat
     1381 catgcttatt tgccaatcta tgcaattcaa aaccatttat gcgaatgcgg tattgctgga
     1441 gaagttattc aaggcccttt tcaagctgct gtgaaaaatg tagctgcaaa ccaattagta
     1501 gatagaattg atgtacgttt aggggatggc ttaagtgtta ttcaacctga ggacgtaatc
     1561 gataatatca caatttgtgg tatgggcgga ccacttattg ctaaaatttt aaaagatggg
     1621 caagataagt taagtcaaca tccaaggttg attttacaaa gtaatatcca aactgaaaat
     1681 ttaagacaaa cattacaaca attgaattat gaaataattg atgaaataat aatggaagaa
     1741 aaaggccata tttatgaaat tgttgtagca gaatattcta cagaattaat agagctatca
     1801 tctgatgaat tgaaattcgg accaaaattg ctgaacaaca aaaatgaata ttttataaaa
     1861 aaatggcaaa gagagttaga agcactttat catattaaat cgaaactcaa tactgaacaa
     1921 catcatcaac gtttagctca aattaatgac gaaattgctg taattgaaag ggtgttatag
     1981 taatgaaaat agctgattta atgacattgt tagatcatca tgtgccattt agtactgctg
     2041 aatcttggga taatgtagga ttgttaatag gtgatgaaga tgttgaagtt actggtgttt
     2101 taacagcatt agactgtacg ttggaagtag taaatgaagc aatcgaaaaa ggttataata
     2161 ctattattag tcatcatcct ctaatcttta aaggcgtaac atcattaaaa gctaatggtt
     2221 atggtttgat cattagaaaa ctaattcaac atgacattaa tttaatagcg atgcatacaa
     2281 atttagatgt aaatccgtat ggtgtcaata tgatgttggc gaaggcgatg ggtttgaaga
     2341 acatttcaat aataaataat caacaagatg tatactataa agttcaaaca tatataccta
     2401 aggataatgt tggaccattt aaagataagc ttagtgaaaa tggattagcg caagaaggta
     2461 attatgaata ttgtttcttt gaaagtgaag gaagagggca attcaaacca gttggtgaag
     2521 ctaatccaac aataggacaa attgataaaa ttgaatatgt agatgaagtt aaaattgaat
     2581 ttatgataga tgcatatcaa aagtcaaggg ctgagcaatt aattaaacaa taccatccat
     2641 atgaaacacc ggtatttgat tttattgaga taaaacaaac atccctttat ggacttggcg
     2701 ttatggcaga agtggataat caaatgacat tggaagattt cgcagctgat attaaatcta
     2761 aattaaatat cccaagtgtc cgttttgttg gtgagtctaa tcagaaaatt aaacgtattg
     2821 caattattgg tggttcaggt attggatatg aatatcaagc tgtccaacaa ggcgcagatg
     2881 tctttgttac aggtgatatt aaacatcatg atgccttaga tgctaaaatt catggtgtga
     2941 atttaattga tattaatcat tacagcgaat atgtgatgaa agaaggttta aaagcgttac
     3001 taatgaattg gttcaataca gaaaaaataa atttagatgt tgaagcatct acaattaata
     3061 cagatccatt tcaatatatt taaactaaaa tctcgggatt tctaaatttt gaaattt
//
LOCUS       DAXWFK010000209         3043 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.211, whole genome shotgun sequence.
ACCESSION   DAXWFK010000209 DAXWFK010000000
VERSION     DAXWFK010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.211"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            55..483
                     /locus_tag="ACGEXS_002453"
     CDS             55..483
                     /locus_tag="ACGEXS_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013435.1"
                     /translation="MTRSALKPFKNKRVMVTGRIQRVLFKNYLDRHSTFKPNVRILLK
                     DVFVSGVSIDHLWLYETNKYYALAMELIHQRVKFSANVVPYYKINRNNNLFVQDYGIK
                     RKGRLITEEAYNQNNQYQDKIYEKLPDIDFRLEDFYSKEN"
     gene            complement(692..1198)
                     /locus_tag="ACGEXS_002454"
     CDS             complement(692..1198)
                     /locus_tag="ACGEXS_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFF8013436.1"
                     /translation="MAHIIRRVSIKDVENFISMLANIYDESPYMFYTPGEYDPSVTSA
                     SKQLEEYITSPHKVIFVAESDEQLVGFAFVNTTPFQRIKHVAKIDLGVKKLYQHRGIG
                     QALLDAIMAWCLNNQIHRIEANVPLNNQPALELFKSADFQIEGVLKDKLFIDGKYYDD
                     YMMAKILN"
     gene            1311..2234
                     /gene="graX"
                     /locus_tag="ACGEXS_002455"
                     /gene_synonym="apsX"
     CDS             1311..2234
                     /gene="graX"
                     /locus_tag="ACGEXS_002455"
                     /gene_synonym="apsX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="auxiliary protein GraX/ApsX"
                     /protein_id="HFF8013437.1"
                     /translation="MKPKVLLAGGTGYIGKYLSEVIENDAELFAISKYPDNKKTDDVE
                     MTWIQCDIFHYEQVVAAMNQIDIAVFFIDPTKNSAKITQSSARDLTLIAADNFGRAAA
                     INQVKKVIYIPGSRYDNETIERLGAYGTPVETTNLVFKRSLVNVELQVSKYDDVRSTM
                     KVVLPKGWTLKNVVNHFIAWMGYTKGTFVKTEKSHDQFKIYIKNKVRPLAVFKIEETA
                     DGIITLILLSGSLVKKYTVNQGKLEFRLIKESAVVYIHLYDYIPRLFWPIYYFIQAPM
                     QKMMIHGFEVDCRIKDFQSRLKSGENMKYTK"
     gene            2250..2924
                     /gene="graR"
                     /locus_tag="ACGEXS_002456"
                     /gene_synonym="apsR"
     CDS             2250..2924
                     /gene="graR"
                     /locus_tag="ACGEXS_002456"
                     /gene_synonym="apsR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor
                     GraR/ApsR"
                     /protein_id="HFF8013438.1"
                     /translation="MQILLVEDDNTLFQELKKELEQWDFNVAGIEDFGKVMDTFESFN
                     PEIVILDVQLPKYDGFYWCRKMREVSNVPILFLSSRDNPMDQVMSMELGADDYMQKPF
                     YTNVLIAKLQAIYRRVYEFTAEEKRTLTWQDAVVDLSKDSIQKGDQTIFLSKTEMIIL
                     EILITKKNQIVSRDTIITALWDDEAFVSDNTLTVNVNRLRKKLSEISMDSAIETKVGK
                     GYMAHE"
     gene            2917..>3043
                     /locus_tag="ACGEXS_002457"
     CDS             2917..>3043
                     /locus_tag="ACGEXS_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFF8013439.1"
                     /translation="MNNLKWVAYFLKSRMNWIFWILFLNFLMLGISLIDYDFPIDS"
BASE COUNT         1118 a          377 c          557 g          991 t
ORIGIN      
        1 aaattaaaat atagattgag cacgaagtga tttgaaataa ggttgtgaaa gggaatgaca
       61 aggtcagcat taaaaccatt taaaaataaa cgcgttatgg ttactggacg tatacaacgt
      121 gttttgttta aaaattattt agatagacat agcacattta agccgaatgt aaggatatta
      181 ttaaaagatg tatttgtttc aggtgtatca atagatcatt tatggttata tgagacaaat
      241 aaatactatg cattggcaat ggaacttatt catcaacgag taaaatttag tgcgaatgtt
      301 gtaccgtatt acaaaataaa tagaaataat aatttattcg tacaagatta tggaattaag
      361 cgtaaaggta ggttaattac tgaagaagct tacaatcaaa acaatcagta tcaggataag
      421 atatatgaaa aattaccgga tatagatttt agactcgaag atttttatag taaggaaaac
      481 taaacaacat atataaagca ccactaatcg tagttgatag ttaatcaatt acacattagt
      541 ggtgttttat tttgaattta aatttgataa atgaataaga gtgtacgtat ttatttgaga
      601 ttagataaca ttgataagta tgagtaatgt attaggtaac cattaaattc gttgttatac
      661 gattctgata caagattatg ataaaatagc tttaattaag aattttagcc atcatatagt
      721 catcataata tttaccatcg ataaataact tatcttttaa aacgccttcg atttgaaaat
      781 cggcactttt aaaaagctcg agggcaggtt ggttattgag tggtacattt gcttcaattc
      841 ggtgtatttg attgtttaaa caccaagcca taatggcatc aagaagtgct tggccaattc
      901 cacgatgttg atataatttc tttacaccta aatcaatttt agcaacatgt ttaatgcgtt
      961 gaaatggtgt cgtattaaca aaggcaaagc caacgagttg ttcatcactt tcagcaacga
     1021 agatgacttt atgcggagaa gtgatatatt cttctaattg tttactagcc gatgtgacgc
     1081 taggatcata ttctcctggt gtgtagaaca tatacggaga ttcgtcgtat atgttcgcta
     1141 acattgaaat gaaattttct acatctttga tactaactct acgtataata tgggccataa
     1201 aaagcctcca gtattttgaa attttaaaac atttgctact attataatat atatgtaact
     1261 aaaaggtgga gtaatatgtt ttttggttac agtttaaagg agtattttag atgaaaccta
     1321 aagttttatt agcaggtgga acaggatata ttggtaagta tttaagtgaa gtgattgaaa
     1381 atgatgctga actttttgct atatcaaaat atccagacaa taaaaaaaca gatgatgttg
     1441 aaatgacttg gattcagtgt gatatatttc attacgaaca ggttgttgca gcaatgaatc
     1501 aaatagatat tgctgtattc tttatcgacc caacaaagaa ttctgccaaa ataacacaat
     1561 catcagcaag agatttaaca ttaatcgcag cagataattt tggtcgagca gcggctatca
     1621 atcaagtaaa aaaagtaatc tacatacctg ggagtcgtta tgataatgaa acaattgaac
     1681 gcctaggtgc atatggtaca cctgtagaaa caacaaattt agtttttaaa cgttctttag
     1741 ttaatgtaga attacaagtt tcaaagtatg atgatgttag atcaacgatg aaggtagttt
     1801 taccaaaggg atggacatta aagaacgttg taaaccattt tattgcatgg atgggttaca
     1861 ctaaaggaac ttttgtgaaa acagaaaaat cacatgatca atttaagata tatattaaga
     1921 ataaggtgcg accgctcgca gtatttaaaa tagaagaaac agctgatgga ataataactt
     1981 taattttatt gagtggaagt ttagtgaaaa aatatacagt taatcaaggg aagttagaat
     2041 ttagattaat caaagagtcg gcagtcgttt atatacatct atacgattat atccctcgat
     2101 tattttggcc gatttattac tttatacaag caccaatgca aaaaatgatg attcatggct
     2161 ttgaagttga ctgccggatt aaagattttc aaagtcgatt aaaatcagga gaaaatatga
     2221 aatatactaa atgatattgg gtgatatgga tgcaaatact actagtagaa gatgacaata
     2281 ctttgtttca agaattgaaa aaagaattag aacaatggga ttttaatgtt gctggtattg
     2341 aagatttcgg caaagtaatg gatacatttg aaagttttaa tcctgaaatt gttatattgg
     2401 atgttcaatt acctaaatat gatgggtttt attggtgcag aaaaatgaga gaagtttcca
     2461 acgtaccaat attattttta tcatctcgtg ataatccaat ggatcaagtg atgagtatgg
     2521 aacttggcgc agatgattat atgcaaaaac cgttctatac caatgtatta attgctaaat
     2581 tacaagcgat ttatcgtcgt gtctatgagt ttacagctga agaaaaacgt acattgactt
     2641 ggcaagatgc tgtcgttgat ctatcaaaag atagtataca aaaaggtgat cagacgattt
     2701 tcctgtccaa aacagaaatg attatattag aaattcttat taccaaaaaa aatcaaatcg
     2761 tttcgagaga tacaattatc actgcattat gggatgatga agcatttgtt agtgataata
     2821 cgttaacagt aaatgtgaat cgtttacgaa aaaaattatc tgaaattagt atggatagtg
     2881 caatcgaaac aaaagtagga aaaggatata tggctcatga ataatttgaa atgggtagct
     2941 tattttttga aatctcgcat gaactggata ttttggatat tgtttttaaa cttccttatg
     3001 ttaggcatta gtctaatcga ttatgatttt ccaatagaca gtt
//
LOCUS       DAXWFK010000210         2982 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.212, whole genome shotgun sequence.
ACCESSION   DAXWFK010000210 DAXWFK010000000
VERSION     DAXWFK010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.212"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..196
                     /locus_tag="ACGEXS_002458"
     CDS             <1..196
                     /locus_tag="ACGEXS_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFF8013440.1"
                     /translation="IEMNQGISFLPLYITTNSDYNISVINTKILQAPISFTYIYSKKE
                     SPEILVFIKSFKKYIANEQL"
     gene            complement(255..2864)
                     /gene="clpB"
                     /locus_tag="ACGEXS_002459"
     CDS             complement(255..2864)
                     /gene="clpB"
                     /locus_tag="ACGEXS_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFF8013441.1"
                     /translation="MDINKMTYAVQSALQQAVELSQQHKLQNIEIEAILSAALNESES
                     LYKSILERANIEVDQLNKAYEDKLNTYASVEGDNIQYGQYISQQANQLITKAESYMKE
                     YEDEYISMEHILRSAMDIDQTTKHYINNKVEVIKEIIKKVRGGNHVTSQNPEVNYEAL
                     AKYGRDLVEEVRQGKMDPVIGRDEEIRNTIRILSRKTKNNPVLIGEPGVGKTAIVEGL
                     AQRIVKKDVPESLLDKTVFELDLSALVAGAKYRGEFEERLKAVLKEVKESDGRIILFI
                     DEIHMLVGAGKTDGAMDAGNMLKPMLARGELHCIGATTLNEYREYIEKDSALERRFQK
                     VAVSEPDVEDTISILRGLKERYEVYHGVRIQDRALVAAAELSDRYITDRFLPDKAIDL
                     VDQACATIRTEMGSNPTELDQVNRRVMQLEIEESALKNESDNASKQRLQELQEELANE
                     KEKQAALQSRVESEKEKIANLQEKRAQLDESRQALEDAQTNNNLEKAAELQYGTIPQL
                     EKELRELEDNFQDEQGEDTDRMIREVVTDEEIGDIVSQWTGIPVSKLVETEREKLLHL
                     SDILHKRVVGQDKAVDLVSDAVVRARAGIKDPNRPIGSFLFLGPTGVGKTELAKSLAA
                     SLFDSEKHMIRIDMSEYMEKHAVSRLIGAPPGYIGHDEGGQLTEAVRRNPYSVILLDE
                     VEKAHTDVFNVLLQILDEGRLTDSKGRSVDFKNTIIIMTSNIGSQVLLENVKETGEIT
                     ESTEKAVMTSLNAYFKPEILNRMDDIVLFKPLSIDDMSMIVDKILTQLNIRLLEQRIS
                     IEVSDDAKAWLGQEAYEPQYGARPLKRFVQRQIETPLARMMIKEGFPEGTTIKVNLNS
                     DNNLTFNVEKIHE"
BASE COUNT          856 a          586 c          393 g         1147 t
ORIGIN      
        1 aatcgaaatg aatcaaggta tatcattcct gcctctttat ataacaacaa atagtgatta
       61 caatatttcg gttattaaca cgaaaatttt acaagcacct atttcattta catatatata
      121 cagcaaaaaa gaaagcccag aaatattggt gtttattaaa tcatttaaaa agtatattgc
      181 caatgaacaa ttataataaa tttcaaatct aaaaaaccaa gaatgcgatt aatcatcaca
      241 ttcttggttc aattttattc atgaattttt tcaacattaa acgttaagtt attgtctgaa
      301 tttaaattaa ctttaatcgt tgtaccttct gggaatccct ctttaatcat catacgtgct
      361 aatggtgttt caatttggcg ttgtacaaaa cgttttaatg gtcttgcacc gtattgaggt
      421 tcataagctt cttgacctag ccaagcttta gcatcatcag aaacttcaat tgagattcgt
      481 tgttctaata atcttatatt taattgcgtt aagattttat ctacaatcat actcatgtca
      541 tcaatagata atggtttaaa taatacgata tcatccatac gattcaaaat ttctggtttg
      601 aaatatgcat ttaaacttgt cataacagct ttttctgttg attctgtaat ttcaccagtc
      661 tcttttacgt tttctaataa aacttgagat ccaatattac ttgtcataat aataatagta
      721 tttttaaaat caacgctacg tcctttagaa tcagttaaac ggccttcatc taaaatttgc
      781 aataatacat taaagacgtc agtatgcgct ttttcaacct catctaataa aataactgag
      841 tatggattac gacgaaccgc ttcagttaat tgaccccctt catcatgtcc aatatatcct
      901 ggaggtgccc ctatcaatct tgatactgca tgtttttcca tatattcact catatcaata
      961 cgaatcatat gtttttcaga atcaaataat gatgcagcta atgatttagc taattcagtt
     1021 ttacctactc cagttggacc taggaataag aaactaccaa taggtctgtt tggatcttta
     1081 atacctgctc ttgctctaac tactgcatct gaaaccaggt caaccgcttt atcttgacct
     1141 acaacacgtt tatgcaagat gtcacttaag tgaagtaatt tttcacgttc tgtttcaact
     1201 aattttgaaa ctggtatgcc tgtccattgg ctgacaatat cgccaatttc ttcgtctgtt
     1261 acaacttcac gaatcattcg atctgtatct tcaccttgct catcttggaa attatcctct
     1321 aattctctaa gttctttttc caattgagga attgttccat attgtagttc agcagctttt
     1381 tctaaattgt tatttgtttg tgcatcttcc aacgcttgtc tactttcatc tagttgcgca
     1441 cgtttttctt gtaaatttgc tattttttct ttttctgatt ctacacgaga ttgaagtgct
     1501 gcttgtttct ctttttcatt ggcaagctct tcttgtagtt cttgtaatct ctgtttgctc
     1561 gcattgtcag attcattttt aagtgcgctt tcttcaattt ctaattgcat gacacgtcta
     1621 ttaacttgat ccaattcagt tggatttgat cccatttccg tacgaattgt tgcacatgct
     1681 tggtcaacta aatcaatcgc tttatctggt aaaaaacgat cagtgatgta acgatcagac
     1741 aattcagcgg cagcaactaa ggctctatct tgaatacgca caccatgata cacttcatat
     1801 cgttctttta aaccacgtaa aattgaaatt gtatcttcaa catcaggctc actaactgct
     1861 actttttgga aacgacgctc taatgccgaa tctttttcaa tatattctcg atattcattt
     1921 aaagttgttg caccaataca atgtaactct cctcgtgcta acattggttt tagcatgttg
     1981 cctgcatcca tggcaccatc tgttttacca gcacctacaa gcatatggat ttcatcaata
     2041 aataatataa ttctaccatc agactcttta acttctttta ggactgcttt taatctctct
     2101 tcaaattcac cacgatattt agcgcccgct actaatgcgc ttaaatctaa ctcaaaaaca
     2161 gttttatcta ataatgattc tggcacatct ttcttaacta tacgttgcgc taatccttca
     2221 acaattgcag ttttaccaac acctggttca ccaatgagca cagggttgtt tttagtttta
     2281 cgacttaaaa tacgaatcgt atttcgaatt tcttcatctc ttcctataac aggatccatt
     2341 ttaccttgtc taacttcttc tactaagtcg cggccatatt tagctaatgc ttcgtaatta
     2401 acttctggat tttgtgatgt cacgtgattt ccccctctta cttttttaat aatttctttg
     2461 ataacttcta ctttattatt tatataatgt tttgttgttt gatcaatgtc cattgccgaa
     2521 cgtaaaatat gctccattga aatatattca tcttcatatt ctttcatgta tgattcagcc
     2581 ttagttatca attggtttgc ttgttggcta atatattgac catattgtat attgtcacct
     2641 tctacagatg catacgtgtt tagtttgtct tcataagctt tgtttaattg atctacctca
     2701 atatttgctc gttctaaaat acttttatat aagctttcac tttcatttaa ggcagcgctt
     2761 aaaattgcct caatttctat attttgtaat ttatgttgct gactcagttc aactgcttgt
     2821 tgtaaagcac tttgaacagc atatgtcatt ttatttatat ccatataatt tcacctctaa
     2881 tttgtagagt tggtcaagtt gcacttttga ctttgacttt ctttgacctt aattatatta
     2941 tagtcccaat ataatcattt atcaactctt ttacacttaa at
//
LOCUS       DAXWFK010000211         2970 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.213, whole genome shotgun sequence.
ACCESSION   DAXWFK010000211 DAXWFK010000000
VERSION     DAXWFK010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.213"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            219..2324
                     /gene="rsp"
                     /locus_tag="ACGEXS_002460"
     CDS             219..2324
                     /gene="rsp"
                     /locus_tag="ACGEXS_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator Rsp"
                     /protein_id="HFF8013442.1"
                     /translation="MTCQLKIHHTLSTIPSRNINNIMVLFSLTSKLNITINGETKDVS
                     NYIILINHGDIYNINHGENIIELMIPVFYFYQQDDDFFNGYLDRHLLQSSNYIKSLIA
                     DLISTPTSSSLMGKNIGQSIIDTLLKEAFIRIDHEYLPNIALSNPVFIDCVNYIHDNI
                     DAHLSLKDIAMHCNISESYCSNLFVRYLSMNFKDYFTSIKLVNAINLLLSTKHSITTV
                     SELAGFNSHTNFANQFKNYLHFSPKQFRSLVSKITEPPQIHFQQDNVSQFTELISTID
                     LTAQLATNTTDIHIDDFNPKDRSQRAKVFVRFSNFNELFQFIFNEYYDINFEHLPKPV
                     VFIDDIHDIEISQTNYNLLNRCFEKLFEKNIGLAIAIKSTQQFETMKQLILTFLQGNQ
                     DYKTSKKLVKFMLVFCSNSMTAEEIHLCHLKIKNKNKEIKYSVTVDGFLETYSTVEQV
                     YDVMQRLKFHYYFIDIENSKTATHLITKNQHYHQTDTHFEQYKKFILDSGISSTQFVY
                     NNLSVSGFKYTNDGKNPIQLSDIVYHLIALLRYGGGISYQLLDDHSNYISLYNKYGSP
                     LPLMHLYKMFRPFVNEDIEITNNYVLSRKDNNYHFLLFNKINDRYMSDVKQDFIFHNE
                     LPQDSLMIIKTLNHEHGSIQHLLPISDQLVYIEKEILDELDKTNYPKTELAVQEETGR
                     TFELKLNHDEVKYICFKPS"
     gene            2395..2817
                     /locus_tag="ACGEXS_002461"
     CDS             2395..2817
                     /locus_tag="ACGEXS_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HFF8013443.1"
                     /translation="MSNSQAIQAIENVLATSKVGVLSTAYNNKPNSRYMVFYNDGLTL
                     YTKTNIHSAKVKEIKDNPAAYVLLGYNDTTNRSFVEMEATIEIVTEQEVIDWLWETQD
                     KSFFSSKEDPELCVLRVVPQSIKLMNDKSLDTPIKIDL"
BASE COUNT         1103 a          450 c          395 g         1022 t
ORIGIN      
        1 aaattttgct tactatcttt ctaattttac ttttatattg tgcgcaatgc ataaaaacaa
       61 gcgttttcct aggagttttc attaatttct tttatttttc actattacct tttcaaaaat
      121 attcaactat aataggtata ctatccatga ttttaaaaat ttccatgttt tacatttaaa
      181 tgatagattt caatatatat taggcaggag agagaaatat gacatgccaa cttaaaatac
      241 atcatacatt atcgacaata ccaagtcgta atattaataa tattatggtg ctattttcac
      301 ttacaagtaa actcaatatt acgattaatg gtgaaaccaa agacgtaagt aattatatta
      361 tcttaattaa ccatggtgat atttataata ttaaccacgg cgaaaatata atcgaattaa
      421 tgatacccgt tttttatttt tatcaacaag atgatgattt ctttaatggt tatttagatc
      481 gtcatttatt acaatcaagt aattatatta agtcattaat tgccgattta atcagtacac
      541 caacaagttc ttctttaatg gggaaaaata taggtcaaag tattatagac actttactta
      601 aagaagcttt tataagaata gatcatgaat atttaccaaa tattgcttta agcaatccgg
      661 tgttcataga ctgtgtaaac tatattcatg acaatattga tgcgcacttg tctttaaaag
      721 atatagcgat gcattgcaac atctctgaat catattgttc taatcttttt gttcgatatt
      781 tgagtatgaa ttttaaagat tattttacaa gtattaaact tgttaacgcc atcaatttat
      841 tactttctac aaaacattct attacaactg tttctgaatt ggccggtttt aacagtcata
      901 caaattttgc aaatcaattt aagaattact tgcattttag tcctaaacag ttccgttcac
      961 tcgtttccaa gattactgaa ccaccacaaa tacacttcca acaagataac gtatcacaat
     1021 tcactgagtt aatttcaaca attgatttaa cagctcaact ggctacaaat acaacagata
     1081 ttcatataga tgattttaat ccgaaagatc gaagtcagcg tgccaaagta tttgttcgat
     1141 ttagtaattt caatgaacta tttcaattta ttttcaatga atattacgat attaactttg
     1201 agcacttacc aaaacctgtc gttttcattg acgatattca cgatattgag attagccaaa
     1261 cgaattacaa cctgttaaat agatgttttg aaaagctttt cgaaaaaaat ataggtttag
     1321 caatcgctat taaatcaaca caacaattcg aaacgatgaa gcaattaata ctaacatttt
     1381 tacaaggcaa tcaagactat aagacaagca agaaattagt taaatttatg ctcgtatttt
     1441 gctctaattc tatgacagca gaagaaattc atttatgtca tcttaaaatt aaaaacaaaa
     1501 ataaagaaat taaatatagt gtgacagtcg atggcttttt agaaacttat tcaaccgtgg
     1561 aacaagttta tgatgtcatg cagcgtctta aattccatta ttactttatt gatattgaaa
     1621 attcaaaaac tgcaacacat cttatcacta aaaaccaaca ctatcatcaa actgacactc
     1681 attttgaaca gtataagaaa tttattttag attcaggtat atcatcaaca caatttgtat
     1741 ataataacct gtctgtaagc ggatttaaat atactaatga tggtaagaat ccaattcaat
     1801 tatctgacat agtgtatcac ttaatcgcat tattacgata tggcggtggc attagctatc
     1861 aattattaga tgaccattca aattatattt ccttgtacaa caaatatggt agccccctac
     1921 cattaatgca tttatataaa atgtttagac cttttgttaa tgaagatatt gaaattacaa
     1981 ataattatgt attgagtcgt aaagataata actaccattt cttattattc aataaaatta
     2041 atgatcgata tatgtcagac gtaaaacaag atttcatttt ccataatgaa ttacctcaag
     2101 actctttgat gattattaaa acattgaatc atgaacatgg ttcaattcaa catttgcttc
     2161 caataagcga tcaacttgtt tatatagaaa aagaaatttt agatgaatta gacaaaacga
     2221 attaccctaa aacggagctt gcagttcaag aagaaactgg tcgtacattt gaactcaagt
     2281 taaatcacga cgaggttaaa tatatttgct ttaaaccaag ctaaatacta acagtcctct
     2341 tgtgtttagt ttcttacgtt aaaggctatt tatatcataa ggagatgatt tgtaatgagt
     2401 aactcacaag caattcaagc aattgaaaac gtgttagcaa cgtcaaaagt tggtgtatta
     2461 tcaactgcat ataataataa acctaatagt agatatatgg tcttttataa tgatggtctt
     2521 actttatata ctaaaacgaa tatccattct gctaaggtca aagaaattaa agataatcca
     2581 gcagcatatg ttttgttagg ctataacgac acaactaatc gtagtttcgt tgagatggaa
     2641 gcgacaatcg aaatcgttac agaacaagaa gtgattgatt ggctatggga aacacaagac
     2701 aaaagctttt tcagttcaaa agaagaccca gagttatgtg ttttaagagt agttccgcaa
     2761 tccattaagc taatgaatga taaatcatta gatacaccta tcaaaatcga tttataacac
     2821 aaagtgtata taggaaataa cttttatgaa ttctagatat aacaatgtta aatacttaaa
     2881 ataactcgct ataattaaag tgtttaatat gtttacaatt caatttgcaa tatattgttt
     2941 tttagacaca ctttattcac atttaacgtg
//
LOCUS       DAXWFK010000212         2944 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.214, whole genome shotgun sequence.
ACCESSION   DAXWFK010000212 DAXWFK010000000
VERSION     DAXWFK010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.214"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(180..2789)
                     /gene="adhE"
                     /locus_tag="ACGEXS_002462"
                     /gene_synonym="adhC"
     CDS             complement(180..2789)
                     /gene="adhE"
                     /locus_tag="ACGEXS_002462"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFF8013444.1"
                     /translation="MLTIPEKENRGSKEQEVAIMIDALADKGKKALEALSKKSQEEID
                     HIVHQMSLAAVDQHMVLAKLAHEETGRGIYEDKAIKNLYASEYIWNSIKDNKTVGIIG
                     EDKEKGLTYVAEPIGVICGVTPTTNPTSTTIFKAMIAIKTGNPIIFAFHPSAQESSKR
                     AAEVVLEAAMKAGAPKDIIQWIEVPSIEATKQLMNHKGIALVLATGGSGMVKSAYSTG
                     KPALGVGPGNVPSYIEKTAHIKRAVNDIIGSKTFDNGMICASEQVVVIDKEIYKDVTN
                     EFKAHQAYFVKKDELQRLENAIMNEQKTGIKPDIVGKSAVEIAELAGIPVPENTKLII
                     AEISGVGSDYPLSREKLSPVLALVKAQSTKQAFQICEDTLHFGGLGHTAVIHTEDETL
                     QKDFGLRMKACRVLVNTPSAVGGIGDMYNELIPSLTLGCGSYGRNSISHNVSATDLLN
                     IKTIAKRRNNTQIFKVPAQIYFEENAIMSLTTMDKIEKVMIVCDPGMVEFGYTKTVEN
                     VLRQKTEQPQIKIFSEVEPNPSTNTVYKGLEMMVDFQPDTIIALGGGSAMDAAKAMWM
                     FFEHPETSFFGAKQKFLDIGKRTYKIGMPENATFICIPTTSGTGSEVTPFAVITDSET
                     NVKYPLADFALTPDVAIIDPQFVMSVPKSVTADTGMDVLTHAMESYVSVMASDYTRGL
                     SLQAIKLTFEYLKSSVEKGDKVSREKMHNASTLAGMAFANAFLGIAHSIAHKIGGEYG
                     IPHGRANAILLPHIIRYNAKDPQKHALFPKYEFFRADTDYADIAKFLGLKGNTTEALV
                     ESLAKAVYELGQSVGIEMNLKSQGVSEEELNESIDRMAELAFEDQCTTANPKEALISE
                     IKDIIQTSYDYKQ"
BASE COUNT          839 a          560 c          463 g         1082 t
ORIGIN      
        1 aatgagtatc aatcttaata ttttctttat ttaaaaaaat gattgtctag tttgtatctc
       61 tctgaagatt tggcaataaa taaaagccga taaccgtata atgattatcg acttaaagtt
      121 tatgtggcat tttttacttt tgtaatttca ggtgagttag atgattatta tcagatagat
      181 tattgcttat aatcatatga tgtttgaatg atatctttga tttcactgat tagtgcttct
      241 ttaggattag cagttgtaca ttgatcttca aatgcgagct ctgccattct atcaattgac
      301 tcatttaatt cttcttcaga cacaccttgt gatttcaaat tcatttcaat tccgactgat
      361 tgacctaatt cgtagacagc tttagctaat gattctacga gtgcttctgt cgtattacct
      421 tttaatccta agaatttggc aatatctgca taatctgtat ctgctctgaa gaactcatat
      481 ttagggaata atgcatgttt ttgcgggtct ttggcattat aacggataat atgcggtagt
      541 aatatcgcat tcgctctacc atgcggaata ccatattcgc caccaatttt atgcgcaatt
      601 gagtgtgcaa tgcctaagaa tgcatttgca aatgccatac cagccaaagt tgatgcgtta
      661 tgcattttct ctcttgaaac tttatcaccc ttttcaacag atgattttaa atattcgaac
      721 gtcaatttaa tcgcttgtag actcaaacct cttgtgtagt ctgaagccat tacagataca
      781 tatgattcca ttgcatgcgt tagtacatcc attcctgtat ctgctgtaac gctttttggc
      841 acactcatca caaattgagg gtcaataatt gcaacgtcag gtgttaaagc aaaatcagcc
      901 aacggatatt ttacatttgt ttcactatct gtgataactg caaatggtgt tacttctgaa
      961 cctgtacctg atgtcgtagg gatacaaatg aacgtcgcat tttcaggcat gcctatttta
     1021 taagtacgtt taccgatgtc taggaacttt tgtttagcac cgaagaatga tgtctcaggg
     1081 tgttcaaaga acatccacat tgcttttgca gcatccatcg ctgaaccacc accaagtgca
     1141 atgattgtat ccggttggaa atcaaccatc atttccagac ctttatatac tgtattagtt
     1201 gatgggttcg gttcgacttc gctaaatatt ttaatttgag gctgttccgt tttttgtctt
     1261 aatacattct caactgtttt tgtataaccg aattctacca taccagggtc acagacaatc
     1321 atcacttttt caatcttgtc cattgttgtt agactcatga ttgcattttc ttcaaaataa
     1381 atttgagcag gcaccttgaa aatttgagta ttattacgtc gtttagcaat cgttttaatg
     1441 tttaataaat ctgtcgcact aacattatgt gaaattgagt ttctaccgta ggaaccacaa
     1501 cctaatgtta aagacggaat caattcgtta tacatatcac caatacctcc aaccgctgat
     1561 ggtgtattta caagtacacg acaagctttc attcttagtc caaaatcttt ttgtaatgtt
     1621 tcatcttctg tatggataac ggctgtgtgt cctaatccac caaaatgtag tgtgtcttca
     1681 caaatttgaa atgcttgttt tgtagattgg gcttttacta aggctaatac tggagataat
     1741 ttttcacgag ataacggata gtctgaacct acaccgctaa tttcggctat gataagtttt
     1801 gtattttcgg ggacaggtat acctgctaat tcagctattt caactgcaga tttaccgaca
     1861 atatcaggct taatacctgt tttttgttca ttcataattg cattttctaa gcgttgtaat
     1921 tcatcttttt taacaaagta tgcttgatgt gctttaaatt cattagtaac atctttataa
     1981 atttctttat caatgactac aacttgttca gaagcacaaa tcataccatt atcaaatgtt
     2041 tttgaaccaa tgatatcatt tactgcacgt ttaatgtgtg ctgttttttc aatgtaagac
     2101 ggcacgttac ctggtcccac acctaatgcc ggtttgccag ttgaatatgc agacttaacc
     2161 atgcccgaac cacctgttgc tagaactaat gcaatacctt tgtgattcat taattgtttt
     2221 gttgcttcga tagaaggcac ttcaatccac tgaataatat ctttaggtgc acctgccttc
     2281 attgccgctt ctaatacaac ttctgctgca cgcttcgacg attcttgtgc acttggatgg
     2341 aatgcaaaaa tgattggatt tcctgtctta attgcaatca tcgctttaaa aatagttgtc
     2401 gacgtaggat ttgttgttgg cgtaacacca caaataacac caattggttc cgctacatac
     2461 gttaatcctt tttctttatc ttcaccaata atccctactg tcttattgtc ttttattgaa
     2521 ttccatatat attcagaagc gtataaattt ttaatcgctt tatcttcgta tatacctctt
     2581 ccagtttctt catgtgctaa ttttgctagc accatatgtt gatcaacagc tgctaagctc
     2641 atttgatgaa caatatgatc aatttcttct tgtgactttt tagataatgc ttctaatgct
     2701 tttttccctt tgtcagctag agcatcaatc ataattgcca cttcttgttc tttcgatcca
     2761 cgattttctt tttcaggtat agttaacata tacaaccact cctttatact ttgtgaatta
     2821 tttcacaaac attatagtac atgtctctca ggatataaag aaaattctat acaaaaaagt
     2881 ttaatttcga atattatttg aacaaatatc aaattttaaa ataaatgttt tcatgaaatc
     2941 attg
//
LOCUS       DAXWFK010000213         2941 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.215, whole genome shotgun sequence.
ACCESSION   DAXWFK010000213 DAXWFK010000000
VERSION     DAXWFK010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.215"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..>2941
                     /gene="sdrC"
                     /locus_tag="ACGEXS_002463"
     CDS             <1..>2941
                     /gene="sdrC"
                     /locus_tag="ACGEXS_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MSCRAMM family adhesin SdrC"
                     /protein_id="HFF8013445.1"
                     /translation="TATNRKGMIPNRLNKFSIRKYSVGTASILVGTTLIFGLSGHEAK
                     AAEHTNGELNQSKNETTAPSENKTTKKVDSRQLKDNTQTATADQPKVTMSDSATVKET
                     SSNMQSPQNATANQSTTKTSNVTTNDKSSTTYSNETDKSNLTQAKDVSTTPKTTTIKP
                     RTLNRMAVNTVAAPQQGTNVNDKVHFSNIDIAIDKGHVNQTTGKTEFWATSSDVLKLK
                     ANYTIDDSVKEGDTFTFKYGQYFRPGSVRLPSQTQNLYNAQGNIIAKGIYDSTTNTTT
                     YTFTNYVDQYTNVRGSFEQVAFAKRKNATTDKTAYKMEVTLGNDTYSEEIIVDYGNKK
                     AQPLISSTNYINNEDLSRNMTAYVNQPKNTYTKQTFVTNLTGYKFNPNAKNFKIYEVT
                     DQNQFVDSFTPDTSKLKDVTDQFDVIYSNDNKTATVDLMKGQTSSNKQYIIQQVAYPD
                     NSSTDNGKIDYTLDTDKTKYSWSNSYSNVNGSSTANGDQKKYNLGDYVWEDTNKDGKQ
                     DANEKGIKGVYVILKDSNGKELDRTTTDENGKYQFTGLSNGTYSVEFSTPAGYTPTTA
                     NVGTDDAVDSDGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQDSTEK
                     GIKGVKVTLQNEKGEVIGTTETDENGKYRFDNLDSGKYKVIFEKPAGLTQTGTNTTED
                     DKDADGGEVDVTITDHDDFTLDNGYYEEETSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDNDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDND
                     SDSDSDSDSDAGKHTPAKPMSTVKDQHKTAKALPETGSENNNSNNGTLFGGLFAALGS
                     LLLFGRR"
BASE COUNT         1154 a          503 c          544 g          740 t
ORIGIN      
        1 agacagcaac aaatagaaaa ggcatgatac caaatcgatt aaacaaattt tcgataagaa
       61 agtattctgt aggtactgct tcaattttag tagggacaac attgattttt gggttaagtg
      121 gtcatgaagc taaagcggca gaacatacga atggagaatt aaatcaatca aaaaatgaaa
      181 cgacagcccc aagtgagaat aaaacaacta aaaaagttga tagtcgtcaa ctaaaagaca
      241 atacgcaaac tgcaactgca gatcagccta aagtgacaat gagtgatagt gcaacagtta
      301 aagaaactag tagtaacatg caatcaccac aaaacgctac agctaatcaa tctactacaa
      361 aaactagcaa tgtaacaaca aatgataaat catcaactac atatagtaat gaaactgata
      421 aaagtaattt aacacaagca aaagatgttt caactacacc taaaacaacg actattaaac
      481 caagaacttt aaatcgcatg gcagtgaata ctgttgcagc tccacaacaa ggaacaaatg
      541 ttaatgataa agtacatttt tcaaatattg acattgcgat tgataaagga catgttaatc
      601 agactactgg taaaactgaa ttttgggcaa cttcaagtga tgttttaaaa ttaaaagcaa
      661 attacacaat cgatgattct gttaaagagg gcgatacatt tacttttaaa tatggtcaat
      721 atttccgtcc aggatcagta agattacctt cacaaactca aaatttatat aatgcccaag
      781 gtaatattat tgcaaaaggt atttatgata gtacaacaaa cacaacaaca tatactttta
      841 cgaactatgt agatcaatat acaaatgtta gaggtagctt tgaacaagtt gcatttgcga
      901 aacgtaaaaa tgcaacaact gataaaacag cttataaaat ggaagtaact ttaggtaatg
      961 atacatatag cgaagaaatc attgtcgatt atggtaataa aaaagcacaa ccgcttattt
     1021 caagtacaaa ctatattaac aatgaagatt tatcgcgtaa tatgactgca tatgtaaatc
     1081 aacctaaaaa tacatatact aaacaaacgt ttgttactaa tttaactgga tataaattta
     1141 atccaaatgc aaaaaacttc aaaatttacg aagtgacaga tcaaaatcaa tttgtggata
     1201 gtttcacccc tgatacttca aaacttaaag atgttactga tcaattcgat gttatttata
     1261 gtaatgataa taaaacagct acagtcgatt taatgaaagg ccaaacaagc agcaataaac
     1321 aatacatcat tcaacaagtt gcttatccag ataatagttc aacagataat ggaaaaattg
     1381 attatacttt agacactgac aaaactaaat atagttggtc aaatagttat tcaaatgtga
     1441 atggctcatc aactgctaat ggcgaccaaa agaaatataa tctaggtgac tatgtatggg
     1501 aagatacaaa taaagatggt aaacaagatg ccaatgaaaa agggattaaa ggtgtttatg
     1561 tcattcttaa agatagtaac ggtaaagaat tagatcgtac gacaacagat gaaaatggta
     1621 aatatcagtt cactggttta agcaatggaa cttatagtgt agagttttca acaccagccg
     1681 gttatacacc gacaactgca aatgtaggta cagatgatgc tgtagattct gatggactaa
     1741 ctacaacagg tgtcattaaa gacgctgaca acatgacatt agatagtgga ttctacaaaa
     1801 caccaaaata tagtttaggt gattatgttt ggtacgacag taataaagat ggtaaacaag
     1861 attcgactga aaaaggaatt aaaggtgtta aagttacttt gcaaaacgaa aaaggcgaag
     1921 taattggtac aactgaaaca gatgaaaatg gtaaataccg ctttgataat ttagatagtg
     1981 gtaaatacaa agttatcttt gaaaaacctg ctggcttaac tcaaacaggt acaaatacaa
     2041 ctgaagatga taaagatgcc gatggtggcg aagttgatgt aacaattacg gatcatgatg
     2101 atttcacact tgataatggc tactacgaag aagaaacatc agatagcgac tcagattctg
     2161 acagcgattc agactcagat agcgactcag attcagacag cgattcagac agcgactcag
     2221 actcagatag cgattcagat tcagacagcg actcagactc agacagcgat tcagattcgg
     2281 atagcgactc agactcagat agcgactcag attcggatag cgactcagac tcagatagcg
     2341 attcagattc agatagcgat tcggactcag acagtgattc agattcagac tcagatagcg
     2401 actcagattc tgacagcgat tcagactcag acagcgactc agactcagac agtgattcag
     2461 attcagacag cgactcagat tcagatagcg actcagactc agatagcgac tcagattcag
     2521 atagcgattc ggactcagac aacgactcag attcagatag cgattcagat tcagatagcg
     2581 actcagattc ggacagcgat tcagactcag atagcgattc agactcagac agcgattcag
     2641 attcagatag cgactcagac tcagatagcg actcagactc ggatagcgat tcagattcag
     2701 acagcgactc agattcagat agcgattcgg actcagacaa cgactcagat tcagatagcg
     2761 attcagattc agatgcaggt aaacatactc cggctaaacc aatgagtacg gttaaagatc
     2821 agcataaaac agctaaagca ttaccagaaa caggtagtga aaataataat tcaaataatg
     2881 gcacattatt cggtggatta ttcgcggcat taggatcatt attgttattc ggtcgtcgta
     2941 a
//
LOCUS       DAXWFK010000214         2902 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.216, whole genome shotgun sequence.
ACCESSION   DAXWFK010000214 DAXWFK010000000
VERSION     DAXWFK010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.216"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            277..741
                     /gene="accB"
                     /locus_tag="ACGEXS_002464"
     CDS             277..741
                     /gene="accB"
                     /locus_tag="ACGEXS_002464"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFF8013446.1"
                     /translation="MNFKEIKELIEILDKSTLTEINIEDTKGKVTLKKEKETEIITPQ
                     ISQMPVEAAAMPMPQAQSTDSNKTEAPKPTSDNHKTINAPMVGTFYKSPSPDEEAYVQ
                     VGDTVSNETTVCILEAMKLFNEIQAEISGEIVEILVEDGQMVEYGQPLFKVK"
     gene            741..2096
                     /gene="accC"
                     /locus_tag="ACGEXS_002465"
     CDS             741..2096
                     /gene="accC"
                     /locus_tag="ACGEXS_002465"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFF8013447.1"
                     /translation="MKKVLIANRGEIAVRIIRACRDLGIQTVAIYSEGDKDALHTQIA
                     DEAYCVGPTLSKDSYLNIPNILSIATSTGCDGVHPGYGFLAENADFAELCEACQLKFI
                     GPSYQSIQKMGIKDVAKAEMIKANVPVVPGSDGLMKDVSEAKKIAKKIGYPVIIKATA
                     GGGGKGIRVARDEKELETGFRMTEQEAQTAFGNGGLYMEKFIENFRHIEIQIVGDSYG
                     NVIHLGERDCTIQRRMQKLVEEAPSPILDDETRREMGNAAVRAAKAVNYENAGTIEFI
                     YDLNDNKFYFMEMNTRIQVEHPVTEMVTGIDLVKLQLQVAMGDVLPYKQEDIKLTGHA
                     IEFRINAENPYKNFMPSPGKIEQYLAPGGYGVRIESACYTNYTIPPYYDSMVAKLIIH
                     EPTRDEAIMAGIRALSEFVVLGIDTTIPFHIKLLNNDIFRSGKFNTNFLEQNSIMNDE
                     G"
     gene            2111..2473
                     /locus_tag="ACGEXS_002466"
     CDS             2111..2473
                     /locus_tag="ACGEXS_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFF8013448.1"
                     /translation="MVKVTDYSNSKLGKVEIAPEVLSVIASIATSEVEGITGHFAELK
                     ETNLEKVSRKNLSRDLKIESKEDGIYIDVYCALKHGVNISKTANKIQTSIFNSISNMT
                     AIEPKQINIHITQIVIEK"
     gene            2533..>2902
                     /gene="nusB"
                     /locus_tag="ACGEXS_002467"
     CDS             2533..>2902
                     /gene="nusB"
                     /locus_tag="ACGEXS_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFF8013449.1"
                     /translation="MSRKESRVQAFQTLFQLEMKDSDLTINEAISFIKDDNPDLDFEF
                     IHWLVSGVKDHEPVLDETISPYLKDWTIARLLKTDRIILRMATYEILHSDTPAKVVMN
                     EAVELTKQFSDDDHYKFINGV"
BASE COUNT         1085 a          417 c          554 g          846 t
ORIGIN      
        1 tatatatcta catatcatat tgttgaggat aaaacataat agctaataaa aataacattg
       61 atatgtttaa ctgaatgcat aaaattttta atcaagagtt aagatgagtc aatgttaagt
      121 aataaataca caagtgtttt taatttaaaa tgtggtttta ttataacttt gggatagact
      181 tataatgtcg ccagcaatat gaacatgctt gaattgaaga gttgtctcaa gtaaaataga
      241 cgggtagatg aaaacaaact gaaggagtca gtaataatga actttaaaga aatcaaagaa
      301 ttaattgaaa ttctggataa atcaacttta acggaaatca atattgaaga tactaaaggc
      361 aaagtgacgc ttaagaaaga aaaagaaact gagattatca cgccacaaat ctcacaaatg
      421 ccagttgaag ctgcggcaat gcctatgcct caagcacaat caactgatag caataaaact
      481 gaagctccaa agccaacttc agataatcac aaaacaatta atgcacctat ggtaggtaca
      541 ttttacaaat cgccatctcc agacgaagaa gcatatgtgc aagttgggga cactgtttca
      601 aatgaaacaa cagtgtgtat tttagaggca atgaaactat ttaatgaaat tcaagcagaa
      661 atttcaggtg aaattgttga aatcttagta gaagacggac aaatggtaga gtatggccaa
      721 ccgttattta aggtgaaata atgaaaaagg ttttaattgc aaaccgcggt gaaatcgcag
      781 ttaggattat tcgcgcttgt cgtgatttag gcatccaaac tgttgcaatc tattctgaag
      841 gggataaaga tgcgctacat actcaaattg ctgatgaagc atattgcgta ggtcccactt
      901 tgtctaaaga ttcatattta aatattccga acatcttatc tattgcaact tctacaggtt
      961 gtgatggcgt tcatccgggt tatggctttt tagctgaaaa tgctgatttt gcagaattat
     1021 gcgaagcatg ccaattgaag ttcattggac caagttatca atctatccaa aaaatgggta
     1081 tcaaagatgt tgctaaggca gaaatgatca aagccaatgt tccagttgtt cctggtagtg
     1141 acggtttaat gaaagacgtc tcagaagcta agaaaatcgc caaaaaaatt ggctatccgg
     1201 tcatcattaa agctactgct ggcggtggcg gaaaaggtat ccgtgttgct cgtgatgaaa
     1261 aagaacttga aactggcttc cgaatgacag aacaagaagc tcaaactgca tttggtaatg
     1321 gtggacttta tatggagaaa ttcatcgaaa acttccgcca tattgaaatc caaattgttg
     1381 gggacagcta tggtaatgta attcatttag gagaacgtga ttgtacaatt caaagacgta
     1441 tgcagaaatt agtggaagaa gcaccttccc caattttaga tgatgaaaca cgtcgtgaaa
     1501 tgggaaatgc cgcagttcgt gcagcgaaag ctgtaaatta tgaaaatgcg ggaacaattg
     1561 agtttatata tgatttaaat gataataaat tttattttat ggaaatgaat acacgtattc
     1621 aagtagaaca tcctgtaact gaaatggtaa caggaattga tttagttaaa ttacaattac
     1681 aagttgctat gggtgacgtg ttaccgtata aacaagaaga tattaaatta acaggacacg
     1741 caattgaatt tagaattaat gctgaaaatc cttacaagaa ctttatgcca tcaccaggta
     1801 aaattgagca atatcttgca ccaggtggat atggtgttcg aatagagtca gcatgttata
     1861 ctaattatac gataccgcca tattatgatt cgatggtagc gaaattaatc atacatgaac
     1921 cgacacgaga tgaagcgatt atggctggca ttcgtgcact aagtgaattt gtggttcttg
     1981 gtattgatac aactattcca ttccatatta aattattgaa taacgatata tttagaagcg
     2041 gtaaatttaa tacaaacttt ttagagcaaa atagcattat gaatgatgaa ggttaatagg
     2101 aggtcaatcc atggtcaaag taactgatta ttcaaattca aaattaggta aagtagaaat
     2161 agcgccagaa gtgctatctg ttattgcaag tatagctact tcggaagtcg aaggcatcac
     2221 tggccatttt gctgaattaa aagaaacaaa tttagaaaaa gttagtcgta aaaatttaag
     2281 ccgtgattta aaaatcgaga gtaaagaaga tggcatatat atagatgtat attgtgcatt
     2341 aaaacatggt gttaatattt caaaaactgc aaacaaaatt caaacgtcaa tttttaattc
     2401 aatttctaat atgacagcga tagaacctaa gcaaattaat attcacatta cacaaatcgt
     2461 tattgaaaag taatgtcata cctaattcag taattaaata aagaaaaata caaacgtttg
     2521 aaggagttaa aaatgagtcg taaagaatcc cgagtgcaag cttttcaaac tttatttcaa
     2581 ttagaaatga aggacagtga tttaacgata aatgaagcga taagctttat taaagacgat
     2641 aatccagatt tagacttcga atttattcat tggctagttt ctggcgttaa agatcacgaa
     2701 cctgtattag acgagacaat tagtccttat ttaaaagatt ggactattgc acgtttatta
     2761 aaaacggatc gtattatttt aagaatggca acatatgaaa tattacacag tgatacacct
     2821 gctaaagtcg taatgaatga agcagttgaa ttaacaaaac aattcagtga tgatgatcat
     2881 tataaattta taaatggtgt at
//
LOCUS       DAXWFK010000215         2900 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.217, whole genome shotgun sequence.
ACCESSION   DAXWFK010000215 DAXWFK010000000
VERSION     DAXWFK010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.217"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            263..532
                     /gene="rpsN"
                     /locus_tag="ACGEXS_002468"
     CDS             263..532
                     /gene="rpsN"
                     /locus_tag="ACGEXS_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFF8013450.1"
                     /translation="MAKKSKIAKERKREELVNKYYELRKELKAKGDYEALRKLPRDSS
                     PTRLTRRCKVTGRPRGVLRKFEMSRIAFREHAHKGQIPGVKKSSW"
     gene            689..1666
                     /gene="guaC"
                     /locus_tag="ACGEXS_002469"
     CDS             689..1666
                     /gene="guaC"
                     /locus_tag="ACGEXS_002469"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFF8013451.1"
                     /translation="MKIFDYEDIQLIPNKCIVESRSECDTTIQFGPKKFKLPVVPANM
                     QTVMNEKLAKWFAENDYFYIMHRFDEEARIPFIKHMQNSGLFASISVGVKKAEFDFIE
                     KLAQEKLIPEYITIDIAHGHSDSVINMIKHIKTHIPDSFVIAGNVGTPEGVRELENAG
                     ADATKVGIGPGRVCITKIKTGFGTGGWQLAALNICSKAARKPLIADGGIRTHGDIAKS
                     IRFGASMVMIGSLFAAHEESPGETVELDGKQYKEYFGSASEFQKGEHKNVEGKKMFVE
                     HKGSLMDTLKEMQQDLQSSISYAGGKDLKSLRTVDYVIVRNSIFNGDRD"
     gene            1688..2704
                     /locus_tag="ACGEXS_002470"
     CDS             1688..2704
                     /locus_tag="ACGEXS_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017174663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFF8013452.1"
                     /translation="MKKFIVTVVAFLSIIIIAPITEFKPFIHLQNEVRQYIDIHINKE
                     TISAENKLDTPKKQQFAFNNIQMNMSKSDVEKTLNKPKRVTFNEYGTKWYTYYDDDYN
                     NFIMISYMKDKVNALYTNQNIITSKSKIKYNTPKSVVRQRLGEPETEIVKGRVRYEQN
                     NKEYDVFHKNHIYTTVFYDKHRRNNVTAVLQVSDAMENRLKEQYGAPSKSLADSFELQ
                     NFDLVNAERKQHQLSTLKYSKQNSETARKHSKDMANNHYFDHTNLKGQSPFDRLKKDG
                     ITFNSAGENLAYGQVSSIYAHQGLMNSIGHRKNILNDTFKILGVGVDFNDEKQPFWTE
                     NYTG"
BASE COUNT         1138 a          348 c          522 g          892 t
ORIGIN      
        1 attttataaa gtcactttgt tagcgaatgc ttgaaagagt atttaatata gtagaattta
       61 aaatttcaaa gcggaattta ataagtacga agtaattctg ggtatgtttt ataaatgttc
      121 gataatacac tttaatctta aatatgatgg tttagaaaat gatttaacaa agaaatgaaa
      181 ctttactgtt gaattatgtg aggattgtgt tattatataa atcgtaataa ttacgatttg
      241 ataaaaagtg aggtaactat atatggctaa gaaatctaaa atagcaaaag agagaaaaag
      301 agaagagtta gtaaataaat attacgaatt acgtaaagag ttaaaagcaa aaggtgatta
      361 cgaagcgtta agaaaattac caagagattc atcacctaca cgtttaacta gaagatgtaa
      421 agtaactgga agacctagag gtgtattacg taaatttgaa atgtctcgta ttgcgtttag
      481 agaacatgcg cacaaaggac aaattccagg tgttaaaaaa tcaagttggt aaaatcaaat
      541 ttcgtacttt agcccattta caatatcaat aaaacaatgt acagtatata cgaatgctat
      601 aaactgaatg ttttctcata ttaataagga aacattcggt ttttaatttg catttaaaaa
      661 taattagttt ttaaaggggc tatttaaagt gaaaatattt gattacgaag atattcaatt
      721 aatacctaat aaatgcatag ttgaaagtag gtctgaatgt gatacaacta tccaatttgg
      781 tccgaaaaaa ttcaagctac ctgtagttcc tgcaaatatg caaacagtta tgaatgagaa
      841 attagcgaaa tggtttgctg aaaatgatta cttttatatc atgcatcgtt ttgatgaaga
      901 agcaagaata ccttttataa aacatatgca aaattcaggc ttatttgcat ctatttcagt
      961 tggtgtaaag aaagcggaat ttgattttat tgaaaagtta gctcaagaaa aattaatccc
     1021 cgaatatatt acaatagata ttgcgcatgg tcactcagat tcagtgataa acatgattaa
     1081 acatataaaa acccatatac ctgatagttt tgttattgct ggtaatgttg gtacgccaga
     1141 aggtgttaga gaattagaaa atgctggtgc tgatgctacc aaagtcggta taggtcctgg
     1201 tagagtttgt attacaaaga ttaaaacagg ttttggtact ggtggttggc agttagcggc
     1261 attaaacata tgtagtaaag cagctcgtaa acctttgatt gccgatggtg gtataagaac
     1321 gcatggcgac attgctaaat caattagatt tggtgcatca atggtcatga ttggttcatt
     1381 atttgcggca cacgaagaat cacctggtga aactgtagaa cttgatggta aacagtataa
     1441 agaatatttt ggtagtgcat ctgaatttca aaaaggcgaa cataaaaatg tagaaggtaa
     1501 aaaaatgttt gtagaacata agggttcatt aatggatacc ttaaaagaaa tgcaacaaga
     1561 tttacaaagc tcaatttcat atgccggtgg aaaagacttg aaatcattac gtactgtaga
     1621 ttatgttatt gttagaaact ctattttcaa cggtgataga gattaatatt tatagtaggt
     1681 gatgtaaatt aaaaaattca tagtaactgt tgttgcgttt ttatcaatta ttatcattgc
     1741 gccaataaca gaatttaaac cattcattca tttacaaaat gaagtaagac aatatattga
     1801 cattcacatc aataaagaaa caatttctgc ggaaaataaa ttggatacac cgaagaaaca
     1861 acaatttgcc tttaataata tacaaatgaa catgtcgaaa tcagatgttg agaaaacatt
     1921 aaataaacca aaaagagtga catttaatga atatggtacg aagtggtata cgtattatga
     1981 tgacgattac aataatttta taatgataag ttacatgaaa gataaagtta atgcgttata
     2041 tacaaatcaa aatataatca cttcaaaatc aaaaattaaa tacaatacac ctaaatcggt
     2101 tgtaaggcaa agattaggcg aaccagaaac agagattgtt aaaggtagag tgcgttacga
     2161 acaaaataat aaagaatatg atgttttcca taaaaatcac atttatacga cggtatttta
     2221 tgataagcat cgacgtaata atgtaacagc tgttttacaa gtaagtgatg ctatggaaaa
     2281 tagattaaaa gaacaatatg gagcaccatc gaaatcgctt gcagatagtt ttgaactaca
     2341 aaattttgat ttagttaatg ctgaaagaaa acaacatcaa ttatctacat tgaagtattc
     2401 taaacagaat tctgaaactg cacgtaagca tagtaaagat atggccaaca atcattattt
     2461 tgatcataca aatttaaaag gtcaatcacc atttgatcga ttgaaaaaag atggtattac
     2521 atttaactca gccggagaga atttagcata tggtcaagtt agtagtatct atgcacatca
     2581 aggattaatg aattctattg gtcacagaaa aaatatttta aatgatacgt ttaaaatatt
     2641 aggtgttggt gttgatttta atgatgaaaa acaacctttt tggacagaaa attatactgg
     2701 ttaatgtttt tggcatgaga ttaaagggta atgtttgtct gtaaggaagt agataaacat
     2761 gatgacaaca gataaaccaa aagatgcgga tatacttgaa cgcgtcaaag atatacttaa
     2821 taaaaaagaa agaaaaaaat aataatggct cgctcctgta aattattacg ggggcgagtt
     2881 ttaatttgtg taacattttg
//
LOCUS       DAXWFK010000216         2846 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.218, whole genome shotgun sequence.
ACCESSION   DAXWFK010000216 DAXWFK010000000
VERSION     DAXWFK010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.218"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(182..265)
                     /locus_tag="ACGEXS_002471"
     tRNA            complement(182..265)
                     /locus_tag="ACGEXS_002471"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(229..231),aa:Leu,seq:caa)
     gene            complement(304..378)
                     /locus_tag="ACGEXS_002472"
     tRNA            complement(304..378)
                     /locus_tag="ACGEXS_002472"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(343..345),aa:Gly,seq:tcc)
     gene            complement(482..556)
                     /locus_tag="ACGEXS_002473"
     tRNA            complement(482..556)
                     /locus_tag="ACGEXS_002473"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(521..523),aa:Gly,seq:tcc)
     gene            complement(564..637)
                     /locus_tag="ACGEXS_002474"
     tRNA            complement(564..637)
                     /locus_tag="ACGEXS_002474"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(603..605),aa:Cys,seq:gca)
     gene            complement(643..714)
                     /locus_tag="ACGEXS_002475"
     tRNA            complement(643..714)
                     /locus_tag="ACGEXS_002475"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(680..682),aa:Gln,seq:ttg)
     gene            complement(726..798)
                     /locus_tag="ACGEXS_002476"
     tRNA            complement(726..798)
                     /locus_tag="ACGEXS_002476"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(763..765),aa:His,seq:gtg)
     gene            complement(801..874)
                     /locus_tag="ACGEXS_002477"
     tRNA            complement(801..874)
                     /locus_tag="ACGEXS_002477"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(840..842),aa:Trp,seq:cca)
     gene            complement(890..970)
                     /locus_tag="ACGEXS_002478"
     tRNA            complement(890..970)
                     /locus_tag="ACGEXS_002478"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(934..936),aa:Tyr,seq:gta)
     gene            complement(976..1051)
                     /locus_tag="ACGEXS_002479"
     tRNA            complement(976..1051)
                     /locus_tag="ACGEXS_002479"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1016..1018),aa:Thr,seq:tgt)
     gene            complement(1059..1131)
                     /locus_tag="ACGEXS_002480"
     tRNA            complement(1059..1131)
                     /locus_tag="ACGEXS_002480"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1096..1098),aa:Phe,seq:gaa)
     gene            complement(1150..1225)
                     /locus_tag="ACGEXS_002481"
     tRNA            complement(1150..1225)
                     /locus_tag="ACGEXS_002481"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1190..1192),aa:Asp,seq:gtc)
     gene            complement(1235..1308)
                     /locus_tag="ACGEXS_002482"
     tRNA            complement(1235..1308)
                     /locus_tag="ACGEXS_002482"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1272..1274),aa:Met,seq:cat)
     gene            complement(1323..1415)
                     /locus_tag="ACGEXS_002483"
     tRNA            complement(1323..1415)
                     /locus_tag="ACGEXS_002483"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1377..1379),aa:Ser,seq:tga)
     gene            complement(1454..1529)
                     /locus_tag="ACGEXS_002484"
     tRNA            complement(1454..1529)
                     /locus_tag="ACGEXS_002484"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1494..1496),aa:Asp,seq:gtc)
     gene            complement(1540..1629)
                     /locus_tag="ACGEXS_002485"
     tRNA            complement(1540..1629)
                     /locus_tag="ACGEXS_002485"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1591..1593),aa:Ser,seq:tga)
     gene            complement(1639..1712)
                     /locus_tag="ACGEXS_002486"
     tRNA            complement(1639..1712)
                     /locus_tag="ACGEXS_002486"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1676..1678),aa:Ile,seq:cat)
     gene            complement(1741..1814)
                     /locus_tag="ACGEXS_002487"
     tRNA            complement(1741..1814)
                     /locus_tag="ACGEXS_002487"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1778..1780),aa:Met,seq:cat)
     gene            complement(1836..1911)
                     /locus_tag="ACGEXS_002488"
     tRNA            complement(1836..1911)
                     /locus_tag="ACGEXS_002488"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1876..1878),aa:Ala,seq:tgc)
     gene            complement(1929..2002)
                     /locus_tag="ACGEXS_002489"
     tRNA            complement(1929..2002)
                     /locus_tag="ACGEXS_002489"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1966..1968),aa:Pro,seq:tgg)
     gene            complement(2012..2085)
                     /locus_tag="ACGEXS_002490"
     tRNA            complement(2012..2085)
                     /locus_tag="ACGEXS_002490"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2049..2051),aa:Arg,seq:acg)
     gene            complement(2104..2192)
                     /locus_tag="ACGEXS_002491"
     tRNA            complement(2104..2192)
                     /locus_tag="ACGEXS_002491"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2156..2158),aa:Leu,seq:taa)
     gene            complement(2203..2277)
                     /locus_tag="ACGEXS_002492"
     tRNA            complement(2203..2277)
                     /locus_tag="ACGEXS_002492"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2243..2245),aa:Gly,seq:gcc)
     gene            complement(2281..2362)
                     /locus_tag="ACGEXS_002493"
     tRNA            complement(2281..2362)
                     /locus_tag="ACGEXS_002493"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2326..2328),aa:Leu,seq:tag)
     gene            complement(2366..2441)
                     /locus_tag="ACGEXS_002494"
     tRNA            complement(2366..2441)
                     /locus_tag="ACGEXS_002494"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2406..2408),aa:Lys,seq:ttt)
     gene            complement(2447..2522)
                     /locus_tag="ACGEXS_002495"
     tRNA            complement(2447..2522)
                     /locus_tag="ACGEXS_002495"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2487..2489),aa:Thr,seq:tgt)
     gene            complement(2539..2614)
                     /locus_tag="ACGEXS_002496"
     tRNA            complement(2539..2614)
                     /locus_tag="ACGEXS_002496"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2579..2581),aa:Val,seq:tac)
     gene            complement(2626..2740)
                     /gene="rrf"
                     /locus_tag="ACGEXS_002497"
     rRNA            complement(2626..2740)
                     /gene="rrf"
                     /locus_tag="ACGEXS_002497"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          799 a          709 c          655 g          683 t
ORIGIN      
        1 ttaaacaatg catagttgct taacttccaa tattgaactc atcattacaa tttgacatag
       61 agtctattaa agcgtgtgcc atttgagtcc acttttattt gtattgtata gagagaaata
      121 aaaagaaacc ttgttttaca aggtttctaa tacgttatgt tatgtaaata acagttaatt
      181 ataccggtgg tcggggtcga accgacactc cacaagtgga acgggatttt gagtcccgcg
      241 cgtctgccaa ttccgccaca ccggcttaat ggtaaacaaa aaacttccct ttggaagcaa
      301 ttatggagcg gaagatagga tttacaccta tacctcgttc cgggaaggaa cgtgttctaa
      361 aagttgaact actcccgcaa atattaaatt atggagcgga agataggatt tacacctata
      421 cctcattcca ggaaggaatg tattctaaga gttgaaatac tcccgcatta ttattaaatt
      481 atggagcgga agataggatt tgcacctata cctcgttccg ggaaggaacg tgttctaaaa
      541 gttgaactac tcccgcataa acctggaggc ggcaaccgga tttgaaccgg tgataaaggt
      601 tttgcagacc tctgccttac cacttggcta tgccgccaat aactgggcta gctggattcg
      661 aaccaacgag tgacggagtc aaagtccgtt gccttaccgc ttggctatag cccattaata
      721 ataagggcgg ctgaagggga tcgaaccctc gaatgtcgga accacaatcc gatgtgttaa
      781 ccacttcacc acagccgcca tggcaggggc agtaggaatc gaacccacac caaaggtttt
      841 ggagacctct attctaccgt tgaactatgc ccctattaaa aataataaat ggaggggggc
      901 agattcgaac tgccgaaccc gaaggagcgg atttacagtc cgccgcgttt agccacttcg
      961 ctacccctcc ataaatggtg ccggccagag gacttgaacc cccaacctac tgattacaag
     1021 tcagttgctc taccaattga gctaggccgg ctaagaaatg gttcaggaca gagtcgaact
     1081 gccgacacat ggagcttcaa tccattgctc taccaactga gctactgaac cataataaaa
     1141 atgtaatgat ggcggtctcg acgggaatcg aacccgcgat ctcctgcgtg acaggcaggc
     1201 gtgttaaccg ctacactacg agacctataa aatattgcgg gaggcggatt tgaaccaccg
     1261 accttcgggt tatgagcccg acgagctacc gaactgctcc atcccgcgat aataaaaaat
     1321 aatggcggag gaagagggat tcgaaccccc gcggcccgtt aaggccctgt cggttttcaa
     1381 gaccgatccc ttcagccgga cttgggtatt cctccattat tataggtaaa tcgctattaa
     1441 ttataaaatt aaatggcggt ctcgacggga atcgaacccg cgatctcctg cgtgacaggc
     1501 aggcgtgtta accgctacac tacgagacca ttagtaaaac ggaggaagag ggattcgaac
     1561 ccccgcgagc cgttaagccc ctgtcggttt tcaagaccga tcccttcagc cggacttggg
     1621 tattcctcca aaattatatg gaccttgcag gactcgaacc tgcgaccgaa cggttatgag
     1681 ccgttagctc taaccaactg agctaaaggt cctaaatata attttacaac taataaatag
     1741 tggcggtgga ggggatcgaa cccccgacct cacgggtatg aaccgtacgc tctagccagc
     1801 tgagctacac cgccttatat agtttgtaaa taatatggtg gagactagcg ggatcgaacc
     1861 gctgacctcc tgcgtgcaaa gcaggcgctc tcccagctga gctaagcccc cataataatt
     1921 acagtatatc gggaagacag gattcgaacc tgcgacccct tggtcccaaa ccaagtgctc
     1981 taccaagctg agctacttcc cgtataatta acgcgcccga taggagtcga acccataacc
     2041 tcttgatccg tagtcaaacg ctctatccaa ttgagctacg ggcgcatatg tttttattga
     2101 aaatggtgcc gaggaccgga atcgaaccgg tacggtgatc actcaccgca ggattttaag
     2161 tcctgtgcgt ctgccagttc cgccaccccg gcactataaa aatggagcag aagacgggat
     2221 tcaaacccgc gaccccaacc ttggcaaggt tgtattctac cgctgaacta cttctgcata
     2281 tgcgggtgaa gggagtcgaa cccccacgcc gtaaggcgct agatcctaag tctagtgcgt
     2341 ctgccaattc cgccacaccc gcaaatggtg agccatagag gattcgaacc tctgaccctc
     2401 tgattaaaag tcagatgctc taccaactga gctaatggct cttccatggt gccggccaga
     2461 ggacttgaac ccccaaccta ctgattacaa gtcagttgct ctaccaattg agctaggccg
     2521 gcaatatgta agaataaatg gtggagaatg acgggttcga accgccgacc ctctgcttgt
     2581 aaggcagatg ctctcccagc tgagctaatt ctccgattta aaactgcctg gcaacgttct
     2641 actctagcgg aacgtaagtt cgactaccat cgacgctaag gagcttaact tctgtgttcg
     2701 gcatgggaac aggtgtgacc tccttgctat agtcaccaga catatgaatg taatttatac
     2761 attcaaaact agatagtaag taaaagtgat tttgcttcgc aaaacattta ttttgattaa
     2821 gtcttcgatc gattagtatt cgtcag
//
LOCUS       DAXWFK010000217         2837 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.219, whole genome shotgun sequence.
ACCESSION   DAXWFK010000217 DAXWFK010000000
VERSION     DAXWFK010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.219"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(49..2049)
                     /locus_tag="ACGEXS_002498"
     CDS             complement(49..2049)
                     /locus_tag="ACGEXS_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFF8013453.1"
                     /translation="MKLRLLWHIVRRQFITQRLVIIPFILAVSVLFMIEYTLVSIGLN
                     SYIKQKNDFLVPFIIIANFFMALLTFIFIFYANHFMMSQRRKEFSIFMTLGMTKKSMR
                     LIVVMETILQFVIISVVSIAGGYLLGAIFFLFIQKIMGSEVATLRYYPFDSVAMFITL
                     IIIAVLMGMLLIFNLFSINFQRPITYQHRSDSSVISRWLRYVLIVIGSAALYLGYFIA
                     LQQDTTFGAFFKIWIVIGLVIIGTYAFFVGISEIIISILQQVSKVYYHPRYFFVVVGM
                     RVRLKMNAVSLATITLLCTFLIVTLTMTLTTYRDMNHTITKLITNDYDLSFSDNSKSQ
                     IERQQTIENIKRDIQGSVNAKDFKVYETTLFRASLEKNRAYYKLKQASDDIPIDFIGN
                     EGAIFSRQSVMITAFTAQDYNRYHQNKLHLDNQSIGMITNVPIFKKQSKVGLNNEVFK
                     VKQLDAHALNLMMIQDSMVLIVKDNNQLQKVKGFYAHEQKNSTISINFNVFGKTKLTT
                     SQIQKLSKKYDASINSKSEMLMVWDRLTGGLIFVGGVVSIVLVIGIFLMMYYKQVSEA
                     YANQHNYDIMKKLGLDNGRIAKITRNQMTFLFAIPITVALIHTLISSNIVYTLLNMIG
                     INNHHIFLTSYVLAVIIISFLYMAMYKITSYIYAKVIHQQRN"
     gene            complement(2046..2801)
                     /locus_tag="ACGEXS_002499"
     CDS             complement(2046..2801)
                     /locus_tag="ACGEXS_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8013454.1"
                     /translation="MLLEVKHVKKVYGKGLNATTALNQMNLSVGAGEFVAIMGESGSG
                     KSTLLNLIASFDGLTEGDIIVDGAHLNNMKNKSKALYRQQMVGFVFQDFNLLPTMTNK
                     ENIMMPLILAGAKRKDIEQRVHQLAVQLHLEGFLNKYPSEISGGQKQRIAIARALVTK
                     PTILLADEPTGALDSKTSKALMMLFQEIHQLEQTILMVTHSNIDASYAERVIFIKDGR
                     LYHEIYRGEESQLAFQQRITDSLALVNGGSVNI"
BASE COUNT          941 a          509 c          407 g          980 t
ORIGIN      
        1 atatttcagt gaaaatacat gattgattgt gatttcactg aaacatggtt aattgcgttg
       61 ttgatgaata actttagcat aaatatagga agttattttg tacatcgcca tatatagaaa
      121 cgaaattata atgacagcta gtacgtaact tgttaaaaat atatgatggt tattaatacc
      181 tatcatattt agtaacgtat atacaatgtt actagaaatt aatgtgtgaa tcagtgctac
      241 tgttattggt attgcgaaca agaaagtcat ttgatttcgt gttatctttg ctattcttcc
      301 attatctaaa ccaagttttt tcataatgtc ataattgtgc tgatttgcat aggcttctga
      361 gacttgttta taatacatca ttagaaatat tccaatgaca agtacaattg atactacgcc
      421 acctacaaat attagtccac ctgtcaaacg atcccaaacc attagcattt ctgatttaga
      481 atttattgac gcatcgtatt ttttcgatag cttttgtatt tgtgatgttg ttaatttcgt
      541 ctttccaaat acattaaagt ttatcgatat tgtcgaattc ttttgttcat gtgcataaaa
      601 tccttttact ttttgtaatt gattgttatc tttaacaatt aaaaccatac tatcttgaat
      661 catcatcaaa tttaacgcat gtgcgtcaag ttgtttcact ttaaagactt cattattaag
      721 accgacctta gactgctttt taaaaatagg tacattcgta atcataccta tactttgatt
      781 gtctaagtgt aatttgtttt ggtgatatcg attataatct tgtgcagtaa aagctgtaat
      841 cattactgat tgtcttgaaa atatcgcgcc ttcattacca atgaagtcta ttggaatatc
      901 atcactagcc tgttttaact tgtaataagc acgattcttt tctaaagaag ctctaaataa
      961 tgtcgtttca tatactttga agtcttttgc attaaccgaa ccctgtatat cacgtttaat
     1021 attctcaatt gtttgttgac gttctatttg tgacttagaa ttgtcgctaa atgacaaatc
     1081 ataatcattc gtaatcaatt tcgtaatggt atgattcata tcacgatagg ttgttaatgt
     1141 cattgtgagc gttacaatca aaaatgtaca cagcaaagtg attgttgcaa gactgactgc
     1201 attcatttta agacgtacac gcatcccaac taccacaaaa aaataccgtg gatgatagta
     1261 aacttttgat acctgctgca atatactaat aattatttca cttataccta caaaaaatgc
     1321 ataagtaccg ataataacta atcctatgac aatccatatt ttaaaaaagg caccaaacgt
     1381 cgtatcttgt tgtaatgcaa taaagtaacc taaatatagt gctgcgcttc ctataacaat
     1441 taaaacgtaa cgcaaccatc gtgatatgac actagaatcg gaacgatgtt gataagttat
     1501 cggccgttga aaattaatac taaacaagtt gaatataagt agcatgccca ttaatacagc
     1561 aatgataatc aaagtaataa acatcgctac agagtcaaat ggataatacc ttaacgtcgc
     1621 aacttcactg cccattattt tctgtataaa caagaaaaat atcgcaccaa gtaagtatcc
     1681 gccggcaata ctaacgactg aaattatcac aaattgtaag atagtttcca tcactacaat
     1741 taaacgcata cttttcttgg tcatgcccaa tgtcataaaa atgctaaact cttttcgtct
     1801 ttgtgacatc ataaagtgat ttgcatagaa aataaaaata aaagttaaaa gcgccataaa
     1861 aaaattagct atgataataa atggtactag gaagtcattc ttctgtttta tgtagctatt
     1921 taacccaatt gacacaagcg tatattcaat catgaatagt acacttaccg ctaaaatgaa
     1981 tggaatgatt acaagtcgct gcgtaataaa ttgacgtcgc actatgtgcc ataacaatct
     2041 taacttcata tattgacact tcctccattc acaagtgcta agctatctgt tattcgttgt
     2101 tgaaaagcta attgactttc ttcaccacga tatatttcat gatatagacg cccatcttta
     2161 ataaaaatga ctcgctctgc ataagacgca tcgatatttg aatgagttac cattaaaatt
     2221 gtctgttcca attgatgaat ctcttgaaat aacatcatca atgcctttga tgtttttgaa
     2281 tcaagtgcac ctgtaggttc atcggctagt aaaatcgtcg gcttagtaac taatgcacgt
     2341 gcaatggcaa tgcgttgctt ctgaccccca gagatttcag aaggatactt gtttaagaat
     2401 ccctctaaat gtaattgtac tgccaactga tgtacccttt gttctatatc ttttcgttta
     2461 gcaccagcta aaattaatgg catcattata ttttctttat tcgtcattgt tggtaaaaga
     2521 ttaaaatctt gaaaaacaaa acctaccatt tgttgacgat acaatgcttt acttttattt
     2581 ttcatattat ttaaatgtgc gccatccaca ataatgtcac cttcagttag tccatcaaaa
     2641 gaagcaatta aatttagtag tgtagacttc cctgacccag actcacccat aattgcaaca
     2701 aattcaccag ctccaactga taaattcatt tgattaagtg ctgtcgtagc attcaaacct
     2761 ttaccataaa ccttttttac atgtttcact tctagcaaca tggcatgcac ctcatttctt
     2821 tgttagttct atattaa
//
LOCUS       DAXWFK010000218         2802 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.220, whole genome shotgun sequence.
ACCESSION   DAXWFK010000218 DAXWFK010000000
VERSION     DAXWFK010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.220"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            15..2288
                     /gene="recJ"
                     /locus_tag="ACGEXS_002500"
     CDS             15..2288
                     /gene="recJ"
                     /locus_tag="ACGEXS_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFF8013455.1"
                     /translation="MIKPKYKWKLTKPAEYISDELTSKLKLTPIVKKILESKSIIDEQ
                     AIESIISDTDINHDALQLSDMTKTIERIKRAIANDEKILVYGDYDADGVTATTILVIT
                     LQLLGAQVGWHIPNRFTEGYGPNELAFRNAHDEGITLIITVDNGIQGHNEIKMVQDLG
                     VDVIVTDHHEIGSTLPEAYAIVHPMHPSFNYPFQQLCGAGVAYKLAQALIENVPDYFK
                     ALVAIGTIADLVSLTDENRSLVKQGLKVLNDQCPTSVKALLKEAGYNDNIDEETIGFI
                     IGPRLNAVGRLDDASLACELLMTDVEEEAAFLAEQVEHFNRERKDIVATITEEAMAMA
                     ETKVKKGDLFLLLAKENWHEGVLGIVASKIVETFALPTLILNIDREQNHAKGSARSID
                     QVSMFEILSAHQELIAKFGGHHMAAGMTMDIENIESLAEGLNKWMKELSKTTSLDPVK
                     QVDVLLTENDITIKNIRDMNRLRPFGTDFSRPIFEMDDLSVSSVKAIGQQKNHLKLTL
                     GESNIAALFWQNGHLEPELQDEQPINILGSVQINEWNGNQSPQIIIQDIAMNEQQILD
                     YRSKRKSLPFTENDENIVVLIHPKSDKVNANEYYYGEEIKQQTDKVVLRDLPTSMEDL
                     SNSLQQLQFSQLYIVLQHNHSIYFDGIPNMDIFKKCYKALITKQETNIQKEGMLLCQH
                     LSVKPDTLKFMLKVFLDLKFVTQEDGLIRINQQPDKRSIDSSKVYQLRQQRMDVEKQL
                     LYQDFSEIKNWIKSQLS"
     gene            2310..>2802
                     /locus_tag="ACGEXS_002501"
     CDS             2310..>2802
                     /locus_tag="ACGEXS_002501"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009559512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFF8013456.1"
                     /translation="MDLKQYVSEVQDWPKPGVSFKDITTIMDNGEAYGYATDKIVEYA
                     KDRDVDIVVGPEARGFIIGCPVAYSMGIGFAPVRKEGKLPREVIRYEYDLEYGTNVLT
                     MHKDAIKPGQRVLITDDLLATGGTIEAAIKLVEKLGGIVVGIAFIIELKYLNGIEKIK
                     DYDV"
BASE COUNT         1055 a          383 c          534 g          830 t
ORIGIN      
        1 aggcgtaaat atatatgatt aaaccgaagt ataagtggaa attaactaaa ccagctgaat
       61 acataagtga tgaattaaca tcaaaattaa agttaacgcc aatcgtaaaa aaaatattag
      121 aaagtaaatc gattattgat gaacaagcaa tagaatccat catttcagat acagatatca
      181 atcatgatgc actgcaattg agcgatatga ctaagactat tgaaagaatt aaaagagcga
      241 tagcgaatga tgagaaaatc ctagtttatg gtgattacga tgccgacggt gttaccgcaa
      301 ctacaatttt agtgataaca ttacaacttt taggtgcaca agtaggatgg catattccta
      361 accgctttac cgaaggatat ggaccaaacg aactagcatt tagaaatgct catgatgaag
      421 gcataacatt aatcataact gttgataatg gcatacaagg tcataatgaa attaaaatgg
      481 ttcaagattt aggagttgat gtcattgtaa ctgatcatca tgaaattggt agtacattac
      541 cagaagcata tgcaatcgta catccaatgc atccatcgtt taattatccg ttccaacaat
      601 tgtgtggtgc aggtgttgca tacaaattag cacaagcatt gattgaaaat gtacctgatt
      661 attttaaagc attagttgcg ataggtacca ttgcggattt agtatcctta actgatgaaa
      721 atcgatcatt agtgaaacaa ggattaaaag tcttaaatga tcaatgtccg acctctgtta
      781 aagcattgct aaaagaagcg ggatacaatg acaatattga tgaagaaaca attggtttta
      841 ttattggacc tagacttaat gctgtaggtc gtttagatga cgcatcatta gcttgcgaat
      901 tgttgatgac tgacgtcgaa gaagaagctg catttttagc agaacaagtt gaacatttta
      961 accgtgaaag aaaagatatt gtagcaacta tcacagaaga agcaatggct atggcagaaa
     1021 cgaaagttaa aaagggagat ttatttttac ttttagcaaa agaaaattgg catgaaggtg
     1081 tcttaggtat tgttgcttcg aaaattgtcg aaacatttgc tttgccaaca ttaattttaa
     1141 atattgatag ggagcaaaat catgcaaaag gctccgccag atcaattgat caagtgtcga
     1201 tgtttgaaat actatcagca catcaagagt taatcgctaa atttggcggc catcatatgg
     1261 ctgcaggtat gacgatggat attgaaaata tagaatcgtt agctgaaggt ttaaataaat
     1321 ggatgaaaga attatctaaa acaacttcgt tagatcccgt aaagcaagta gatgtattat
     1381 taacagaaaa tgatataacg attaaaaata ttcgtgatat gaatcgttta agacccttcg
     1441 gaacagattt ttctagacct atttttgaaa tggatgattt atcagtttct tcagttaagg
     1501 cgataggtca acaaaaaaat catctaaaat tgacacttgg tgaaagtaat atcgccgcgc
     1561 tattttggca aaatggacac ttagaacctg agttacaaga tgaacagcca attaatatat
     1621 taggatctgt tcaaatcaac gaatggaatg gtaatcaatc accgcaaata attattcaag
     1681 atattgcgat gaatgaacag caaatattag attatagaag taagcgaaaa agtttacctt
     1741 ttacagaaaa tgatgaaaat attgtcgtgc ttattcatcc taaaagtgat aaagtaaatg
     1801 cgaatgaata ttattatggt gaagaaatta aacaacaaac tgataaagta gtattaagag
     1861 atttaccaac gtcaatggaa gacttgtcta attccttgca acaactgcaa ttttctcaac
     1921 tttatatagt tttgcaacat aatcattcga tttacttcga tggtatacct aatatggata
     1981 tttttaaaaa gtgttataaa gcattaataa ctaaacaaga aacaaatatc cagaaagagg
     2041 gtatgttatt gtgtcaacat ttaagtgtga aaccagatac acttaaattc atgttgaaag
     2101 ttttcttaga cttaaaattt gtaacacaag aagatggttt aattcgaatc aatcaacaac
     2161 ctgataaaag atcgattgat tccagcaaag tatatcaatt aagacaacaa cgtatggatg
     2221 ttgaaaagca attattatat caagattttt cagaaataaa aaattggata aagtcacaat
     2281 tgtcgtgagc aatttaggag gaaatattaa tggatttaaa gcaatacgta tcagaagttc
     2341 aagattggcc gaaaccaggt gttagtttca aggatattac tacaattatg gataatggtg
     2401 aagcatatgg ctatgcaaca gataaaattg tagaatacgc aaaagacaga gatgttgata
     2461 tcgttgtagg acctgaagcg cgtggcttta tcattggctg tcctgtagct tattcaatgg
     2521 ggattggctt tgcacctgtt agaaaagaag ggaaattacc tcgtgaagtc attcgttatg
     2581 agtatgacct agaatatggt acaaatgttt taacaatgca caaagatgca attaaaccag
     2641 gtcaacgtgt gttaattaca gatgatttat tagctactgg tggtacgatt gaagcagcaa
     2701 taaaattagt tgaaaaatta ggcggtatcg tagtaggtat tgcatttata attgaattga
     2761 aatatttaaa tggtattgaa aaaattaaag attacgatgt ta
//
LOCUS       DAXWFK010000219         2783 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.221, whole genome shotgun sequence.
ACCESSION   DAXWFK010000219 DAXWFK010000000
VERSION     DAXWFK010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.221"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            178..381
                     /locus_tag="ACGEXS_002502"
     CDS             178..381
                     /locus_tag="ACGEXS_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HFF8013457.1"
                     /translation="MTFYNFIMGFQNDNTPFGILAEHVSEDKAFPRLEERHQVIRAYV
                     MSNYTDHQLIETTNRAISLYMAN"
     gene            complement(378..614)
                     /locus_tag="ACGEXS_002503"
     CDS             complement(378..614)
                     /locus_tag="ACGEXS_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013458.1"
                     /translation="MDNHNQCNYVNPQNVSLDWECFIISKSEMLLDGVPNELINTWLD
                     KDIITPFSIRNDEINFKTKDIWDALIHHNWYYSN"
     gene            839..1420
                     /locus_tag="ACGEXS_002504"
     CDS             839..1420
                     /locus_tag="ACGEXS_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFF8013459.1"
                     /translation="MAKTLYLMRHGQTLFNFKGLIQGFGDSPLTELGIAQAQKARSYY
                     ETKGINFDLYASSTQERASDTLENVAPNQSYQRFKGLKEWHFGIFEGESVYLFDNLYK
                     PEDLFGDRIVPFKGEARQQVEERIVKTLHDIMSQTKNNALVVSHGTIMGVFLRYCLKL
                     DEALKHNIGNCNILKFEYDNGTFKFVELIDPNL"
     gene            complement(1493..2110)
                     /locus_tag="ACGEXS_002505"
     CDS             complement(1493..2110)
                     /locus_tag="ACGEXS_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE/ArgO family amino acid transporter"
                     /protein_id="HFF8013460.1"
                     /translation="MVTAIIHGFILAIGLILPLGAQNVFIFNQGANQPKYRYVLPAII
                     TAGLSDSLLIIIAVVGVSIIIMSLPVLQAIIYIVGLIFLMYMAWTIWHDKPSTDGETQ
                     IMSPMKQVSFALSVSLLNPHAILDTIGVIGSSAALYSGSNKIAFTIACISVSWLWFFL
                     LAILGKMVGSIDKTGKLLTIINKISSIIIIIVALMILQKLIQLLF"
     gene            complement(2265..2759)
                     /locus_tag="ACGEXS_002506"
     CDS             complement(2265..2759)
                     /locus_tag="ACGEXS_002506"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8013461.1"
                     /translation="MIEIKTLTNNDFNEYKRLVSTVNEEFTQDSHYSQTMTDTLIHDI
                     LNQGSPKCIVFGCYENETLIATAALEQIRYVGKEHKSLIKYNFVTNNDKSINSELINF
                     IINYARQNNYESLLTSIVSNNIGAKVFYSALGFDILGFEKNAIKIGNTYFDEHWLFYD
                     LINK"
BASE COUNT         1008 a          416 c          416 g          943 t
ORIGIN      
        1 tgaaaagaat atttacatac taaaaactct taaagttgta taattcgtta atatttttag
       61 aatcagatat caaaaaaatg tgcaaaatca cttttgcaca tttacaaata ctagttttct
      121 gataagatta ataataacac tttaaatagc gcttaataaa tgaagggggc aagtcttatg
      181 acgttttaca atttcatcat gggttttcaa aatgataaca caccatttgg tatattggcc
      241 gaacacgtta gtgaagataa agcattccct cgattagaag aaagacacca agtaattaga
      301 gcatatgtga tgtctaatta cacagatcat caattaattg aaactacaaa tagagctatt
      361 agcttatata tggcaaatta atttgagtag taccaattat gatgtattag tgcatcccaa
      421 atatcttttg ttttaaagtt tatttcatca tttcttatcg aaaatggtgt aataatgtct
      481 ttatctaacc aagtgttgat aagttcattt ggtacaccat ctaacaacat ttcactttta
      541 ctaattataa aacattccca gtcaagtgaa acattttgtg gattcacata attacattga
      601 ttatgattat ccataaacac tcactccttt aaaattctgt actcttcatt gcgttttacc
      661 ccgtcacatt atcttttaaa ctaaaatcat cattacttat gaaaaaaatg tacatcaaaa
      721 gcaaaggttt tcgctaccga aaaagtttaa ataatggttt aatatatttg gtactcattt
      781 taataaaaag agaatacatt ttgagctatc aatacttttt attgaagagg tgttattgtt
      841 ggctaaaacg ttatatttaa tgcgccacgg acaaactttg tttaatttta agggactaat
      901 tcagggattt ggagattcgc cgctaacaga acttggaatt gctcaagctc aaaaggcacg
      961 tagttattat gaaactaagg ggataaactt cgatttatat gcatcatcaa cgcaagaacg
     1021 cgcaagtgac acacttgaaa atgttgcacc taaccaatcg tatcaacgtt ttaagggact
     1081 gaaggaatgg cattttggaa tatttgaagg tgagtcagtc tatctatttg ataatctata
     1141 caagcctgag gacttattcg gagatcgaat tgttcctttc aaaggagagg caaggcaaca
     1201 agttgaagaa cgcattgtga aaactttaca tgacattatg tctcaaacaa agaataatgc
     1261 attagtcgtg agtcatggaa caataatggg agtattttta agatattgcc ttaaactaga
     1321 tgaagcatta aagcataata tcggtaattg taatatcctg aaatttgaat atgacaatgg
     1381 aacatttaaa tttgttgagt taattgatcc aaatttataa taaataaagc gttatacata
     1441 gacacaagta tgtcttttgt ataacgcttt taaaatacaa ctacataacg ctttagaata
     1501 ataattgaat aagtttttgt aatatcatca gagcaacaat aataattatt atgcttgata
     1561 ttttattgat tattgttaat aattttccgg ttttatcaat tgatccaacc atttttccta
     1621 aaattgcgag taaaaagaac cataaccatg aaacgctaat acaagcgatt gtaaacgcaa
     1681 ttttattgct gccactatat aatgcagcac tactaccaat tactccaatt gtatctaaaa
     1741 tagcatgtgg attgagtaat gaaactgata aagcaaaact tacttgtttc attggagaca
     1801 taatttgagt ttctccatct gttgagggtt tatcatgcca aatggtccaa gccatataca
     1861 tcaagaaaat taaaccaact atataaataa ttgcttgaag tacaggtaaa gacataataa
     1921 tgatagatac tcctaccact gcaataataa taagtaagct gtctgacaac ccggctgtaa
     1981 ttattgcagg caatacatat ctatattttg gttgattagc tccttggtta aaaataaata
     2041 cattttgtgc acctaaaggt aaaattagac caatcgctaa gataaatcca tgaataattg
     2101 cggttaccat taagttttga tacccctaaa acatagaata ttctgaaaaa acttattgtt
     2161 atcatatcaa ataaaaaaca actactcaat agtagagtag ttgtatgaaa aatagaacct
     2221 atatgatttg aatttatgag aaatgtagaa tatataaaac tgtattactt attaatcaaa
     2281 tcataaaaaa gccaatgttc atcgaaatag gtatttccga ttttaattgc atttttctca
     2341 aaaccaagaa tgtcgaatcc tagtgcacta tagaaaactt tagcacctat gttgtttgac
     2401 acaattgatg taagtaaaga ttcgtaattg ttctgccgtg cataattaat aatgaaatta
     2461 atgagctcgc tattaatcga tttatcgtta ttagtaacaa agttgtattt aattaatgat
     2521 ttatgttctt ttccaacgta tcgaatttgt tctaaggcag ctgttgcgat aagtgtttcg
     2581 ttttcataac agccaaatac aatacatttc ggtgaacctt gatttaaaat gtcatgtatt
     2641 aaggtgtcag tcattgtttg actataatgt gaatcttgag tgaattcttc attgactgtc
     2701 gaaacaagtc tcttatactc attaaaatca ttattcgtta atgttttaat ttcaatcatt
     2761 actgacctcc ttaatcatta tta
//
LOCUS       DAXWFK010000220         2775 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.222, whole genome shotgun sequence.
ACCESSION   DAXWFK010000220 DAXWFK010000000
VERSION     DAXWFK010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.222"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(29..106)
                     /locus_tag="ACGEXS_002507"
     CDS             complement(29..106)
                     /locus_tag="ACGEXS_002507"
                     /inference="COORDINATES: protein motif:HMM:NF028245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HFF8013462.1"
                     /translation="MLSFGMFIVTFIGIVVAIINLSNKK"
     gene            complement(407..1009)
                     /locus_tag="ACGEXS_002508"
     CDS             complement(407..1009)
                     /locus_tag="ACGEXS_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFF8013463.1"
                     /translation="MNKISKALTWFIISFIIFHLILFIMWGEHQEYWYLYTGIMLIAG
                     ISYVFYQRDIESKRLLTSIGVGIITAIILIMLQLLFSLITSNLSYSSLIKELARTGVN
                     WKWQMLVTLLFVIPCHELYMRTVLQKELTHFSIPKWVAILITAICSSSLFIYLDNWWI
                     VTFIFVAQVILSLSYEYTRRIATTSVAQIVAIILLLIFNA"
     gene            complement(1024..1200)
                     /locus_tag="ACGEXS_002509"
     CDS             complement(1024..1200)
                     /locus_tag="ACGEXS_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkvS family protein"
                     /protein_id="HFF8013464.1"
                     /translation="MTVAEVGNIVEFMDGLRGRVEKINDNSVIVDLTIMENFNDLDLP
                     EKTVINHKRYKIVE"
     gene            1399..2385
                     /locus_tag="ACGEXS_002510"
     CDS             1399..2385
                     /locus_tag="ACGEXS_002510"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFF8013465.1"
                     /translation="MKFISNNNITDPTLNLAMEEYVLKNLPAEESYFLFYINRPSIIV
                     GKNQNTIEEVNQTYIDAHNIDVVRRISGGGAVYHDTGNLNFSFITDDDGNSFHNFQKF
                     TEPIVQALQSLGVNAELTGRNDIQVGQAKISGNAMVKVKNRMFSHGTLMLNSDLDEVQ
                     NALKVNPAKIKSKGIKSVRKRVANIQEFLNDPLEIEEFKKIILKTIFGETEVEEYKLT
                     DEDWENIEKLSNDKYRTWEWNYGRNPKYNFEREEKFEKGFVQIKFDVKRGKIEHAKIF
                     GDFFGVGDVTDLENALVGCLHDFEHIEEALSEYDLYHYFGDIDRHELIRLMS"
     gene            complement(2466..2684)
                     /locus_tag="ACGEXS_002511"
     CDS             complement(2466..2684)
                     /locus_tag="ACGEXS_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HFF8013466.1"
                     /translation="MKYNTNVKHTTLEAFVTTVNDLGIELIINEALREVRKRQLIELI
                     DDALVNKDEAAFNQYTAEYKNLEAFLGE"
BASE COUNT         1048 a          406 c          400 g          921 t
ORIGIN      
        1 attctatgtc aaagttttga aatgatggtt attttttatt gcttaaattt attattgcta
       61 ctactatacc aatgaaagtg acgataaaca taccgaaact taacattaaa tgtaatgcgt
      121 ttgcaataga tatcacaata ggcttttcct ttcaaaagtg ttaagtaatg ccataggcac
      181 cacccccctt tactcagata gccaccatca attcaacttg ctcaaaaata ttataccatt
      241 tttataaaat atttagtaat aaaaactgac taaaacctaa tctcaataat aaccgcttgc
      301 tgttaaaaaa tattaccact ataatgaagc atgcctcgaa tcttcgtatt gctattctta
      361 agcaaaaaag cttttcttag aacacatacg tctaagaaaa gcttatttaa gcattaaaaa
      421 ttaataacaa tataatagct acaatttgtg caacggatgt tgttgctata cgtcgagtat
      481 actcataact taatgataat atcacttgtg caacaaatat aaatgttact atccaccaat
      541 tatctaaata aataaataat gaactagaac aaattgcagt tattaatata gctacccatt
      601 tcggtattga aaaatgtgtt aattcttttt gtaaaacagt tctcatatat aactcgtgac
      661 atggaatgac aaaaagtaaa gttactaaca tttgccactt ccaattgaca cctgttcttg
      721 ctaattcttt aattaatgaa ctataactta aattagaagt tataagtgag aataaaagtt
      781 gaagcataat taaaataatt gccgtaataa taccaacacc aattgatgta agcaaccgct
      841 tagattcaat atctctttga taaaatacat aactgatacc agcaattagc attatacctg
      901 tatataaata ccagtattct tggtgttcgc cccacataat aaataatatg agatgaaata
      961 taatgaaact tataataaac caagttaaag ccttcgagat tttattcatt ataacttacc
     1021 ttcttattca acaatcttat atcgtttatg attgataaca gttttttccg gtaaatcaag
     1081 gtcattaaaa ttttccataa ttgttaagtc aacaataaca gagttatcgt tgattttttc
     1141 aacacgacct cttaatccat ccataaactc aacaatgtta cccacttctg caacagtcat
     1201 tttaagtcct ccttaaatca taactaaatt atattgtact aaaattcact taacaaataa
     1261 actattaaca atcaaaaata ataaaatgta actttaagcc gatatttaga atgatttgtg
     1321 agtatataat tggcattttg agcaaagtta aggcataaat aagtagagaa tatatataga
     1381 tataagggag agattgtaat gaaattcatt agtaataata atattacaga tccaacttta
     1441 aacttagcaa tggaagaata tgttttaaaa aatttaccag cagaagaaag ttacttttta
     1501 ttttacataa atagaccatc tatcattgtt ggaaagaatc aaaatacgat agaggaagta
     1561 aatcagacat atatcgatgc tcacaacatc gatgtagtaa gaagaatttc tggtggtggc
     1621 gctgtttatc atgatactgg caatttaaac tttagtttta taacagatga tgatggtaac
     1681 agtttccaca atttccaaaa attcactgaa ccaattgttc aagcattaca atctttaggc
     1741 gtaaatgctg aattaactgg tcgtaacgat attcaagttg gacaagcgaa aatctcaggg
     1801 aatgctatgg ttaaagttaa aaatagaatg tttagtcatg gtacgttgat gttaaatagt
     1861 gatttagacg aagttcagaa tgcactaaaa gttaatccag ctaagattaa atcaaaaggt
     1921 attaaatctg ttcgtaaacg ggtagcaaac attcaagaat ttttaaatga ccctttagaa
     1981 attgaagaat ttaaaaaaat catcttgaaa actatctttg gagaaactga agtagaagaa
     2041 tataaattaa cggatgaaga ttgggaaaat attgaaaagt taagtaacga caaatataga
     2101 acttgggaat ggaattatgg tagaaaccct aaatataact ttgaacgcga ggaaaaattt
     2161 gaaaaaggat ttgtacaaat taagtttgat gttaaacgag gtaaaatcga acatgcaaaa
     2221 atattcggtg atttctttgg tgtcggagat gtcactgatc ttgaaaatgc attagttggc
     2281 tgtctacacg actttgagca catagaagaa gcactatcag aatatgattt atatcattat
     2341 ttcggtgaca tagatagaca tgaattaatt agattaatgt cataaacgat taaaggacta
     2401 ttcttacatg tatgagatgt taagaatagt ccttttatga ttgcgttatg gaattcgaat
     2461 caatgttatt caccgagaaa tgcctccaaa tttttgtatt ctgccgtata ttgattaaat
     2521 gctgcttcat ctttattgac gagtgcgtca tctataagtt ctatgagctg tcgttttctt
     2581 acctctcgaa gtgcttcatt gataattaat tcaataccca aatcattgac agttgtgaca
     2641 aacgcttcta aagttgtatg tttaacatta gtattgtatt tcatagaaac cactaccttt
     2701 ctttaattta taataagtat acatttttct aattttgaaa tcaatgaata ttctgactat
     2761 tagttaaaaa ttatt
//
LOCUS       DAXWFK010000221         2731 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.223, whole genome shotgun sequence.
ACCESSION   DAXWFK010000221 DAXWFK010000000
VERSION     DAXWFK010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.223"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            21..1031
                     /gene="sspA"
                     /locus_tag="ACGEXS_002512"
     CDS             21..1031
                     /gene="sspA"
                     /locus_tag="ACGEXS_002512"
                     /EC_number="3.4.21.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-specific serine endopeptidase SspA"
                     /protein_id="HFF8013467.1"
                     /translation="MKGKFLKVSSLFVATLTTATLVSSPAANALSSKAMDNHPQQTQS
                     SKQQTPKIQKGGNLKPLEQREHANVILPNNDRHQITDTTNGHYAPVTYIQVEAPTGTF
                     IASGVVVGKDTLLTNKHVVDATHGDPHALKAFPSAINQDNYPNGGFTAEQITKYSGEG
                     DLAIVKFSPNEQNKHIGEVVKPATMSNNAETQVNQNITVTGYPGDKPVATMWESKGKI
                     TYLKGEAMQYDLSTTGGNSGSPVFNEKNEVIGIHWGGVPNEFNGAVFINENVRNFLKQ
                     NIEDIHFANDDQPNNPDNPDNPNNPDNPNNPDEPNNPDNPNNPDNPDNGDNNNSDNPD
                     AA"
     gene            1113..2294
                     /gene="sspB"
                     /locus_tag="ACGEXS_002513"
                     /pseudo
     CDS             1113..2294
                     /gene="sspB"
                     /locus_tag="ACGEXS_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease staphopain B"
     gene            2332..2661
                     /gene="sspC"
                     /locus_tag="ACGEXS_002514"
     CDS             2332..2661
                     /gene="sspC"
                     /locus_tag="ACGEXS_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphostatin B"
                     /protein_id="HFF8013468.1"
                     /translation="MYQLQFINLVYDTTKLTHLEQTNINLFIGNWSNHQLQKSICIRH
                     GDDTSHNQYHILFIDTAHQRIKFSSIDNEEIIYILDYDDTQHILMQTSSKQGIGTSRP
                     IVYERLV"
BASE COUNT         1068 a          468 c          428 g          767 t
ORIGIN      
        1 aattttttgg aggtttttag atgaaaggta aatttttaaa agttagttct ttattcgttg
       61 caactttgac aacagcgaca cttgtgagtt ctccagcagc aaacgcgtta tcttcaaagg
      121 ctatggacaa tcatccacaa caaacgcagt caagcaaaca gcaaacacct aagattcaaa
      181 aaggcggtaa ccttaaacca ttagaacaac gtgaacacgc aaatgttata ttaccaaata
      241 acgatcgtca ccaaatcaca gatacaacga atggtcatta tgcacccgta acttatattc
      301 aagttgaagc acctactggt acatttattg cttccggtgt agttgtaggt aaagatactc
      361 ttttaacaaa taaacacgtc gtagatgcta cgcacggtga tcctcatgct ttaaaagcat
      421 tcccttctgc aattaaccaa gacaattatc caaatggtgg tttcactgct gaacaaatca
      481 ctaaatattc aggcgaaggt gatttagcaa tagttaaatt ctcccctaat gagcaaaaca
      541 aacatattgg tgaagtagtt aaaccagcaa caatgagtaa taatgctgaa acacaagtta
      601 accaaaatat tactgtaaca ggatatcctg gtgataaacc tgtagcaaca atgtgggaaa
      661 gtaaaggaaa aatcacttac ctcaaaggcg aagctatgca atatgattta agtacaactg
      721 gtggtaattc aggttcacct gtatttaatg aaaaaaatga agtgatcgga attcattggg
      781 gcggtgtacc aaatgaattt aatggtgcgg tatttattaa tgaaaatgta cgcaacttct
      841 taaaacaaaa tattgaagat atccattttg ccaacgatga ccaacctaat aacccagata
      901 atcctgataa ccctaacaat cctgataacc ctaacaaccc agatgaacca aataaccctg
      961 acaaccctaa caaccctgat aatccagaca atggcgataa caataattca gacaatccag
     1021 atgcagctta attaacaatt aagttcgtct agataaaata gaagcaaagt cttagcaacg
     1081 taaaatattt tgatttaaat ggagggtatt atatgaatag ttcatgtaaa tctagagtat
     1141 tcaatattat aagcatcata atggtttcaa tgcttatttt atcactaggc gcatttgcta
     1201 ataacaataa agccaaagcc gattcacact ctaaacagct agaaattaat gttaagagtg
     1261 acaaagtacc tcaaaaagta aaagatctag cacaacaaca atttgctggt tatgctaaag
     1321 cattagataa acaaagtaat gcaaaaactg gtaaatatga acttggcgaa gcttttaaaa
     1381 tttataaatt taatggtgaa gaagataata gttactatta tccagttata aaagacggta
     1441 aaattgttta tactttaaca cttagtccta aaaataaaga tgatttaaac aaatcaaaat
     1501 aagacatgaa ttacagtgtt aaaatttcaa acttcatcgc taaagattta gaccaaatta
     1561 aagataaaaa ttcaaatatc actgttctta ctgatgaaaa agggttttat tttgaagaag
     1621 atggcaaagt tagattagta aaagctacgc ctctacctgg taatgtaaaa gaaaaagaaa
     1681 gtgctaaaac agtttcagca aaattgaaac aagagttaaa aaatacagta acacctacta
     1741 aagttgaaga aaacgaagcg atacaagaag atcaagttca atatgaaaat acattaaaaa
     1801 acttcaaaat tagagaacaa caattcgata actcatggtg tgcaggattc agtatggcag
     1861 cattattaaa tgcaactaaa aatacagaca cttataatgc acatgatatt atgcgtacat
     1921 tataccctga agtaagtgag caagaccttc ctaattgtgc aacattccct aatcaaatga
     1981 ttgaatacgg taaatcacaa ggcagagata ttcattatca agaaggcgta ccatcatatg
     2041 aacaagttga tcaacttaca aaagataatg taggaattat gatccttgca caaagtgtat
     2101 ctcaaaaccc taatgaccca catttaggac atgcgctagc agttgttggt aatgctaaaa
     2161 ttaatgacca agaaaaactt atttactgga atccttggga tacagaatta tcaatccaag
     2221 atgcagattc aagcctatta catttatcat tcaatcgtga ttataactgg tatggttcaa
     2281 tgataggtta ctaaaaagta atatagatat tgattaaagg caggtaaaac tatgtatcaa
     2341 ctacaattta taaatttagt ttacgacaca accaaactca cacatctaga acaaaccaat
     2401 atcaatttat tcattggtaa ttggagtaat catcaattac aaaaatcaat ttgtatacgt
     2461 catggcgatg atacaagtca caatcaatat catattcttt ttatagatac ggcacatcaa
     2521 cgcattaaat tttcatctat tgataatgaa gaaatcattt atattcttga ttatgatgat
     2581 acacagcata tcctcatgca aacgtcatcc aaacaaggta ttggcacttc gcgaccaatc
     2641 gtttatgagc gcttagtata actaatttaa atgatttcac ttcataaagc gggttggcga
     2701 gaattcaatt tctcaccagc tcgttttttc a
//
LOCUS       DAXWFK010000222         2729 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.224, whole genome shotgun sequence.
ACCESSION   DAXWFK010000222 DAXWFK010000000
VERSION     DAXWFK010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.224"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            24..731
                     /gene="splA"
                     /locus_tag="ACGEXS_002515"
     CDS             24..731
                     /gene="splA"
                     /locus_tag="ACGEXS_002515"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplA"
                     /protein_id="HFF8013469.1"
                     /translation="MNKNVMVKGLTALTILTSLGFAENISNQPHSIAKAEKNVKEITD
                     ATKEPYNSVVAFVGGTGVVVGKNTIVTNKHIAKSNDIFKNRVSAHHSSKGKGGGNYDV
                     KDIVEYPGKEDLAIVHVHETSTEGLNFNKNVSYTKFADGAKVKDRISVIGYPKGAQTK
                     YKMFESTGTINHISGTFMEFDAYAQPGNSGSPVLNSKHELIGILYAGSGKDESEKNFG
                     VYFTPQLKEFIQNNIEK"
     gene            856..1578
                     /gene="splB"
                     /locus_tag="ACGEXS_002516"
     CDS             856..1578
                     /gene="splB"
                     /locus_tag="ACGEXS_002516"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplB"
                     /protein_id="HFF8013470.1"
                     /translation="MNKNVVIKSLAALTILTSVTGIGTTLVEEVQQTAKAENNVTKVK
                     DTNIFPYTGVVAFKSATGFVVGKNTILTNKHVSKNYKVGDRITAHPNSDKGNGGIYSI
                     KKIINYPGKEDVSVIQVEERAIERGPKGFNFNDNVTPFKYAAGAKAGERIKVIGYPHP
                     YKNKYVLYESTGPVMSVEGSSIVYSAHTESGNSGSPVLNSNNELVGIHFASDVKNDDN
                     RNAYGVYFTPEIKKFIAENIDK"
     gene            1636..2355
                     /gene="splC"
                     /locus_tag="ACGEXS_002517"
     CDS             1636..2355
                     /gene="splC"
                     /locus_tag="ACGEXS_002517"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplC"
                     /protein_id="HFF8013471.1"
                     /translation="MNKNIVIKSMAALAILTSVTGINAAVVEETQQIANAEKNVTQVK
                     DTNIFPYNGVVSFKDATGFVIGKNTIITNKHVSKDYKVGDRITAHPNGDKGNGGIYKI
                     KSISDYPGDEDISVMNIEEQAVERGPKGFNFNENVQAFNFAKDAKVDDKIKVIGYPLP
                     AQNSFKQFESTGTIKRIKDNILNFDAYIEPGNSGSPVLNSNNEVIGVVYGGIGKIGSE
                     YNGAVYFTPQIKDFIQKHIEQ"
     gene            2476..>2729
                     /gene="splF"
                     /locus_tag="ACGEXS_002518"
     CDS             2476..>2729
                     /gene="splF"
                     /locus_tag="ACGEXS_002518"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplF"
                     /protein_id="HFF8013472.1"
                     /translation="MNKNIIIKSIAALTILTSITGVGTTMVEGIQQTAKAENTVKQIT
                     NTNVAPYSGVTWMGAGTGFVVGNHTIITNKHVTYHMKVGD"
BASE COUNT         1075 a          380 c          503 g          771 t
ORIGIN      
        1 ataaatttgg aaggaggtaa aatatgaata aaaatgtaat ggttaaaggt ttaactgctt
       61 taactatttt aacatctctt ggttttgctg aaaatatttc taatcagcct cattcaattg
      121 ccaaagcaga aaagaatgtc aaagaaatta ccgatgcaac taaggaacca tacaattcag
      181 tggtagcatt tgtgggtggt actggtgtag ttgttggtaa aaatacaatc gtaactaaca
      241 aacatatcgc taaaagtaat gatattttta aaaatagagt atcagcacat cattcgagta
      301 aaggtaaagg cggaggaaac tacgacgtta aagacattgt agaatatccc ggaaaagaag
      361 accttgcgat agttcatgtt catgaaacaa gtacagaagg tttgaatttt aataagaacg
      421 ttagttatac aaaatttgca gacggagcaa aagtgaaaga tagaatttct gttattggtt
      481 atccaaaggg tgcacaaaca aaatataaaa tgtttgaatc gacaggaacg attaaccata
      541 tcagtggaac gtttatggaa tttgatgcgt atgcacaacc aggtaattca ggatctcctg
      601 tattgaattc taaacatgaa ctgattggta ttttatatgc aggtagtgga aaagatgaat
      661 ctgaaaagaa tttcggtgtt tatttcacac cacaattaaa agaatttatt caaaataata
      721 ttgaaaaata agtatcatca attcattcgt gaagttgatt ttttaaagag tgattaagaa
      781 aacggttaca taattaacta aatatactga attatgtatc tagatatcaa aataattaaa
      841 agagaggaac ttaaaatgaa caaaaacgta gtcatcaaga gtttagcagc attaacaatt
      901 ttaacatctg taacaggtat tggaacaaca ttggttgagg aagtacaaca aactgccaaa
      961 gcagaaaata atgtcacaaa agttaaagat actaatattt ttccatatac tggtgtagtt
     1021 gcttttaaaa gtgcaactgg atttgtagtt ggaaagaata ctattttaac aaataaacat
     1081 gtgtcgaaaa attacaaagt gggcgatcgt attactgcac atccaaatag tgataaaggt
     1141 aatggtggta tttattcgat taaaaagatt attaattatc caggtaaaga agatgtatca
     1201 gtcattcaag ttgaagagcg tgcaatagaa cgtggaccaa aaggctttaa ttttaatgat
     1261 aatgtaacgc cattcaaata tgcggcaggg gctaaagctg gtgagcgaat taaagtgatt
     1321 ggttatccac acccatacaa aaataaatat gttttatatg agtcaactgg ccctgtgatg
     1381 tcagtagaag gtagcagtat tgtatattca gcgcatactg aaagcggaaa ctctggatca
     1441 cctgtattaa acagcaacaa cgaattagtt ggtattcatt ttgcttctga tgtaaaaaat
     1501 gatgataaca gaaatgcata tggcgtctac tttacaccag aaattaaaaa attcattgca
     1561 gaaaacatag ataaataaac aaattgactt taaacgagcg ttgcaacata tctcgaattg
     1621 taaaggagct tgaaaatgaa taaaaatata gtcattaaaa gcatggcagc attagccatt
     1681 ctaacctcag taactggaat aaatgctgca gtcgttgaag agacacaaca aatagcaaat
     1741 gcagagaaga atgttacgca agttaaagat acaaatattt ttccatataa tggcgtcgtt
     1801 tcatttaaag atgcgacagg ttttgtaatt ggaaaaaata caattatcac caataaacat
     1861 gtatcaaaag attataaagt tggcgataga attactgccc atccaaacgg tgacaaagga
     1921 aatggtggta tatataaaat taaaagcatt tctgattatc cgggtgatga agacatctct
     1981 gtcatgaata ttgaagaaca agcagtcgaa cgtggaccaa aaggctttaa ttttaatgaa
     2041 aatgtccaag cattcaattt tgcgaaagat gctaaagttg atgacaaaat taaagttatt
     2101 ggttacccat tacctgctca aaatagtttt aaacagtttg aatctacagg aactataaaa
     2161 agaatcaaag acaatatttt aaattttgat gcatacattg aacccgggaa ttcaggatca
     2221 ccagttctaa attctaacaa tgaggtcata ggtgtggtgt atggcggtat tggaaaaatt
     2281 ggttctgaat ataatggtgc cgtatacttt acgcctcaaa tcaaagattt tattcaaaag
     2341 cacattgaac aataaacaaa tttaaatata caccatgagc atgtgttcaa taattttaat
     2401 gaaaaacatc ggtcgaatat aacataaaaa aacgtctata tcaaaagcat catgaataaa
     2461 cagaggagca gaaaaatgaa taaaaatata atcatcaaaa gtattgcagc attgacgatt
     2521 ttaacatcaa taactggtgt cggcacaaca atggttgaag gtattcaaca aacagccaaa
     2581 gccgaaaata ctgttaaaca aattacaaat acaaatgttg caccatacag tggtgttaca
     2641 tggatgggcg ctggaacagg atttgtagtt ggaaatcata caatcattac caataaacat
     2701 gttacctatc acatgaaagt cggtgatga
//
LOCUS       DAXWFK010000223         2714 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.225, whole genome shotgun sequence.
ACCESSION   DAXWFK010000223 DAXWFK010000000
VERSION     DAXWFK010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.225"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            29..949
                     /gene="lukE"
                     /locus_tag="ACGEXS_002519"
     CDS             29..949
                     /gene="lukE"
                     /locus_tag="ACGEXS_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000916699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukED subunit E"
                     /protein_id="HFF8013473.1"
                     /translation="MLAATLSVGLIAPLASPIQESRANTNIENIGDGAEVIKRTEDVS
                     SKKWGVTQNVQFDFVKDKKYNKDALIVKMQGFINSRTSFSDVKGSGYELTKRMIWPFQ
                     YNIGLTTKDPNVSLINYLPKNKIETTDVGQTLGYNIGGNFQSAPSIGGNGSFNYSKTI
                     SYTQKSYVSEVDKQNSKSVKWGVKANEFVTPDGKKSAHDRYLFVQSPNGPTGSAREYF
                     APDNQLPPLVQSGFNPSFITTLSHEKGSSDTSEFEISYGRNLDITYATLFPRTGIYAE
                     RKHNAFVNRNFVVRYEVNWKTHEIKVKGHN"
     gene            951..1934
                     /gene="lukD"
                     /locus_tag="ACGEXS_002520"
     CDS             951..1934
                     /gene="lukD"
                     /locus_tag="ACGEXS_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukED subunit D"
                     /protein_id="HFF8013474.1"
                     /translation="MKMKKLVKSSVASSIALLLLSNTVDAAQHITPVSEKKVDDKITL
                     YKTTATSDNDKLNISQILTFNFIKDKSYDKDTLVLKAAGNINSGYKKPNPKDYNYSQF
                     YWGGKYNVSVSSESNDAVNVVDYAPKNQNEEFQVQQTLGYSYGGDINISNGLSGGLNG
                     SKSFSETINYKQESYRTTIDRKTNHKSIGWGVEAHKIMNNGWGPYGRDSYDPTYGNEL
                     FLGGRQSSSNAGQNFLPTHQMPLLARGNFNPEFISVLSHKQNDTKKSKIKVTYQREMD
                     RYTNQWNRLHWVGNNYKNQNTVTFTSTYEVDWQNHTVKLIGTDSKETNPGV"
     gene            2176..2319
                     /locus_tag="ACGEXS_002521"
     CDS             2176..2319
                     /locus_tag="ACGEXS_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gallidermin/nisin family lantibiotic"
                     /protein_id="HFF8013475.1"
                     /translation="MEKVLDLDVQVKGNNNTNDSAGDERITSHLFCSFGCEKTGSFNS
                     FCC"
BASE COUNT         1035 a          374 c          461 g          844 t
ORIGIN      
        1 agaaagtgaa acttatgttt aagaaaaaat gttagctgca actttgtcag taggactgat
       61 tgcaccttta gcatctccga ttcaagaatc tagagcaaat actaatattg aaaatattgg
      121 tgatggtgcc gaagtaatca aacgtacgga ggatgtaagt agtaagaaat ggggcgttac
      181 tcaaaatgtc caattcgact ttgtaaaaga taaaaaatat aacaaagacg ctttaattgt
      241 taaaatgcaa ggttttatta attccagaac ttcattttca gatgtgaagg gtagtggata
      301 tgaattaact aaacgaatga tttggccatt ccaatataat ataggactga cgactaaaga
      361 tccaaatgtt agcttaatca attaccttcc taaaaacaaa atagaaacta ctgatgttgg
      421 tcaaacatta ggatataaca ttggaggtaa tttccagtca gcaccatcta taggtggcaa
      481 tggctcattt aattattcta aaacaattag ttatacccaa aagagttatg tcagtgaagt
      541 agacaagcaa aactcaaaat ctgttaaatg gggtgttaaa gcaaacgaat ttgttacgcc
      601 tgatggaaaa aaatctgcgc atgatagata tttattcgta caaagtccaa atggtccaac
      661 aggttcagca agagaatatt ttgctcctga taatcaattg ccacctttag ttcaaagtgg
      721 ctttaatcca tcgtttatca ctacactatc acatgaaaaa ggttcaagtg atacgagtga
      781 atttgaaatt tcatatggta gaaacttaga tattacatat gcgactttat tccctagaac
      841 tggtatttac gcagaaagaa agcataatgc atttgtaaat agaaactttg tagttagata
      901 tgaagttaat tggaaaacac acgaaattaa agtgaaagga cataattaat atgaaaatga
      961 aaaaattagt caaatcatca gttgcttcat caattgcact gcttttgcta tcgaatacag
     1021 ttgatgcagc tcaacatatc acacctgtaa gcgagaaaaa agtagatgac aaaatcactt
     1081 tatacaaaac aacagcaaca tctgataatg ataaattgaa tatttctcaa attttaacgt
     1141 ttaatttcat taaggataaa agttatgaca aagatacgtt agtacttaag gcagccggaa
     1201 acattaattc aggttataaa aagcctaatc caaaagatta caattactca cagttttatt
     1261 ggggcggtaa gtataatgtt tcggttagtt cagaatcaaa tgatgctgta aatgttgttg
     1321 actatgcacc taaaaatcaa aatgaagaat tccaagttca acaaacatta ggttattctt
     1381 atggcggaga tattaatata tctaatggct tatcaggtgg attaaatgga tcaaaatcat
     1441 tttcagaaac gataaattat aaacaagaaa gttacagaac tacgattgat agaaaaacaa
     1501 atcataaatc aattggctgg ggtgttgagg cgcacaaaat tatgaataat ggttggggac
     1561 catatggtag agatagttat gacccaacat atggtaatga actgttttta ggtggtagac
     1621 aaagtagttc aaatgctggt caaaatttct tgccaacaca tcaaatgcct ttattggcgc
     1681 gtggtaactt taacccagaa tttataagcg tactttctca taaacaaaat gatacaaaaa
     1741 aatctaaaat caaagtaact taccaaagag aaatggatag atatactaat caatggaatc
     1801 gactacactg ggttggtaat aactacaaaa atcaaaatac agtaacgttt acatctactt
     1861 atgaagttga ctggcaaaac catactgtta aattaatcgg tacggattct aaagaaacta
     1921 atcctggagt ataactgtta gaaatagagg aagttaaacc cgaaatttaa gagttaaaca
     1981 aaaaatattc acaacatatt aaaaaaagaa gttacattat ttgtttaaac taacaatagt
     2041 accataaaat taatgacgat ttacaaatta ataacaaata attattatta aattcattga
     2101 tttattgtgt ggtaatgatt aatatgacaa ttgaagtggg tcttaacttc aacttttaaa
     2161 tagggggttt tatctttgga aaaagttctt gatttagacg tgcaagttaa aggaaataac
     2221 aacactaatg attcagcggg tgacgaaaga ataactagcc atcttttttg tagctttggt
     2281 tgtgaaaaga cgggtagttt taacagcttc tgttgttaag gatacagcaa atagagcctg
     2341 aaagggctct atttgtcttt ttaaatatat taagtgctaa aaaacggaaa cagtttatta
     2401 atcgccaggt atttttataa ttagccttct atattatcag ttgagtgttt caataaattc
     2461 tgactttggt gatggaaagt tatactttta gttgtaatat agttttcatg acttcctgtt
     2521 aaataagtta ggattatgta attagcctct atctttacat ataagtaatg acaagctttg
     2581 gttattatat tgaccctctt agaacatgtc atttaagaaa ataatcaagt cattgaaatt
     2641 atatgtttat atcctcatct ttaaaataac ttcaattatt atttgctaaa ttttgtataa
     2701 atagaacaaa actg
//
LOCUS       DAXWFK010000224         2691 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.226, whole genome shotgun sequence.
ACCESSION   DAXWFK010000224 DAXWFK010000000
VERSION     DAXWFK010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.226"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..679)
                     /locus_tag="ACGEXS_002522"
     CDS             complement(<1..679)
                     /locus_tag="ACGEXS_002522"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFF8013476.1"
                     /translation="MAKLNVEVFADGADIEEMKAAYKNKQVDGFTTNPSLMAKAGVTD
                     YKAFAEEAVKEIPDASISFEVFADDLETMEKEAAILKQYGENVFVKIPIVNTKGESTI
                     PLIKKLSADNVRLNVTAVYTIEQVKEITEAVTEGVPTYVSVFAGRIADTGVDPLPLMK
                     EAVKVTHSKDGVKLLWASCRELFNVIQADEIGADIITCPADVVKKVNTNLGRDINELS
                     VDTVKGFA"
     gene            939..1241
                     /locus_tag="ACGEXS_002523"
     CDS             939..1241
                     /locus_tag="ACGEXS_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013477.1"
                     /translation="MSRSKKYFYLSSLMIILSFFFNTNNVFLSGLFNSFIKLILFCSV
                     INSIVLILSIIFADRSIKSLKPDADWIRIASKSLPWIILIVILVHIFSIVRTFGFI"
     gene            1497..1862
                     /gene="crcB"
                     /locus_tag="ACGEXS_002524"
     CDS             1497..1862
                     /gene="crcB"
                     /locus_tag="ACGEXS_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFF8013478.1"
                     /translation="MQYVYIFIGGALGALLRYLISFLNTDGGFPIGTLIANLTGAFVM
                     GLLTALTIAFFSNHPTLKKAITTGFLGALTTFSTFQLELIHMFDHQQFITLLLYAVTS
                     YVFGILLCYVGIKLGGGLS"
     gene            1859..2212
                     /locus_tag="ACGEXS_002525"
     CDS             1859..2212
                     /locus_tag="ACGEXS_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HFF8013479.1"
                     /translation="MISIILVMIGGGFGAIARSAITDYFNHKFTSKLPIATLIVNLVG
                     SFLIGLTIGLSISISWFPAFFVTGFLGGLTTFSTLAKELTLMMTPKFNINLFLNYSLL
                     QFIIGFIACYISYHI"
BASE COUNT          823 a          471 c          368 g         1029 t
ORIGIN      
        1 tcgcaaagcc tttaactgta tctactgata attcgttaat atcgcgacct aagtttgtat
       61 tcactttttt cacaacatct gctgggcatg taataatatc tgcaccaatt tcatcagctt
      121 gaatcacatt gaataattcg cggcaacttg cccataataa tttaacgccg tctttactat
      181 gcgtaacttt tacagcctct ttcattaatg gtaatggatc tacgcctgta tctgcaatac
      241 gtcctgcaaa tactgaaaca tatgttggca caccttcagt tactgcttca gttatttctt
      301 taacttgttc aattgtgtaa acagccgtaa cgtttaatct cacattgtca gctgaaagtt
      361 ttttaattaa aggaatcgtt gattcacctt ttgtatttac aataggaatt ttaacaaata
      421 cattttcgcc atattgtttt aaaattgctg cttctttttc catagtttct aaatcgtctg
      481 caaatacttc aaatgaaatt gaagcatctg gaatttcttt cacagcttct tcagcaaaag
      541 ctttgtaatc tgttacgccc gctttagcca ttaaactagg atttgttgta aaaccatcca
      601 cttgtttgtt tttataagct gctttcattt cttcaatatc tgcaccgtcc gcaaatactt
      661 ctacatttag tttagccata taatatagcc tccttgattc ttattaaaat tttaacaaca
      721 tctgcatgtc tttttcttac aaccatttgt aaaaaatgat ttttatttct ttgttatata
      781 aaacatttat atcacgcttt tataatatat agctaatttt ttgcctatat tttttaaaaa
      841 tgactttagc aaacattgtg tctacaagtt aataaaacta ttgttttatg attgtttatg
      901 atacgatatg taaatgataa tcatccagga ggtctagcat gtctcgttca aaaaaatact
      961 tttacttatc tagcttaatg attattttaa gctttttctt taatacaaat aacgttttcc
     1021 taagtggact ttttaattct tttattaaat taatactttt ctgcagtgtt attaactcaa
     1081 ttgtactaat tttgtctata atttttgcag atcgttcaat taaatcacta aagcctgatg
     1141 cagattggat tagaattgcg agtaaaagtt tgccttggat tattctaatt gttattttag
     1201 tacatatctt ttcaattgtt cgtacattcg gttttattta aaaaagttaa tatgtcattg
     1261 tagccttaat acaaaacaat acaatgtatc atgctataat gagtaaaaca atttgataac
     1321 gttgttgcgt ataaaaaata ttagattttc gaaatcataa ctatgcatct aatcgctata
     1381 gttatacaac aagatataac atataatgag gtttgataat gcatcgacaa tttttgtcgt
     1441 cgcgttgcca aaacctcttt tttaaattca aactacttct tttcgaggtg aaccaaatgc
     1501 aatatgtata tatttttatc ggtggtgctt taggcgcttt attacgttac ctcatttctt
     1561 ttctgaatac tgacggaggt tttccaatcg gaacactgat agccaatttg actggtgcct
     1621 ttgtaatggg attgctaaca gccttaacaa ttgcattttt ttcaaaccat ccgaccctaa
     1681 aaaaagctat tacgactggt tttcttggtg ctttaacgac tttttcaaca tttcaattag
     1741 aattaataca tatgtttgat catcaacaat ttataacttt actactatat gctgtaacaa
     1801 gttatgtctt tggtattttg ttatgttacg tcggtataaa actaggtggt ggtttatcat
     1861 gatatcaatc attttagtca tgattggcgg cggttttggc gcaattgcta gaagtgccat
     1921 tactgattat tttaatcata aatttacttc aaagttacct atcgcaacat tgatagtaaa
     1981 tctagttggt agttttttaa ttggattaac tataggctta tcaatttcaa tctcatggtt
     2041 ccctgcgttc tttgttaccg gttttttagg tggcttaaca actttctcaa cgttagccaa
     2101 agaacttaca ctaatgatga cgccaaaatt taatattaac ctttttctca attattcact
     2161 tttacaattc atcattggat ttatagcttg ttatattagc tatcatattt aaaaataaaa
     2221 tgcttcattc agcaaatagg taaattacga caccttcctg aacgaagcat tttttaattt
     2281 tcatgcaaat ttttaagcac catataatgc ctaccaaatt tcaataatct ttgttgccgt
     2341 ttaaataatg tgaatgtcaa taaattctcc aaactagtcg aaaataaagg gagtgggaca
     2401 taaatcccta aaaaaacagc agtaagataa ttttcaatta gaaaatatct tactgctgtt
     2461 ctctatttat acaatacttc gtattgaatg gcttcgcttt cctagggtgc cgtctcagcc
     2521 tcggtcttcg actggcactg ctccctcagg agtctcgcca ttaatactac gtattaacat
     2581 gtaattttac ttttacatac tttaaaaaat aagacacttt gcccaactta cactaccaat
     2641 agaaacctct gttagaattc ctcaaaatga tatttcgcga tatgttaatg a
//
LOCUS       DAXWFK010000225         2671 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.227, whole genome shotgun sequence.
ACCESSION   DAXWFK010000225 DAXWFK010000000
VERSION     DAXWFK010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.227"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(139..699)
                     /gene="icaR"
                     /locus_tag="ACGEXS_002526"
     CDS             complement(139..699)
                     /gene="icaR"
                     /locus_tag="ACGEXS_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ica operon transcriptional regulator IcaR"
                     /protein_id="HFF8013480.1"
                     /translation="MKDKIIDNAITLFSEKGYDGTTLDDIAKSVNIKKASLYYHFDSK
                     KSIYEQSVKCCFDYLNNIIMMNQNKSNYSIDALYQFLFEFIFDIEERYIRMYVQLSNT
                     PEEFSGNIYGQIQDLNQSLSKEIAKFYDESKIKMTKEDFQNLILLFLESWYLKASFSQ
                     KFGAVEESKSQFKDEVYSLLNIFLKK"
     gene            863..2101
                     /gene="icaA"
                     /locus_tag="ACGEXS_002527"
     CDS             863..2101
                     /gene="icaA"
                     /locus_tag="ACGEXS_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase
                     IcaA"
                     /protein_id="HFF8013481.1"
                     /translation="MQFFNFLLFYPVFMSIYWIVGSIYFYFTREIRYSLNKKPDINVD
                     ELEGITFLLACYNESETIEDTLSNVLALKYEKKEIIIINDGSSDNTAELIYKIKENND
                     FIFVDLQENRGKANALNQGIKQASYDYVMCLDADTIVDQDAPYYMIENFKHDPKLGAV
                     TGNPRIRNKSSILGKIQTIEYASLIGCIKRSQTLAGAVNTISGVFTLFKKSAVVDVGY
                     WDTDMITEDIAVSWKLHLRGYRIKYEPLAMCWMLVPETLGGLWKQRVRWAQGGHEVLL
                     RDFFSTMKTKRFPLYILMFEQIISILWVYIVLLYLGYLFITANFLDYTFMTYSFSIFL
                     LSSFTMTFINVIQFTVALFIDSRYEKKNMAGLIFVSWYPTVYWIINAAVVLVAFPKAL
                     KRKKGGYATWSSPDRGNTQR"
     gene            2065..2370
                     /gene="icaD"
                     /locus_tag="ACGEXS_002528"
     CDS             2065..2370
                     /gene="icaD"
                     /locus_tag="ACGEXS_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular adhesion protein IcaD"
                     /protein_id="HFF8013482.1"
                     /translation="MVKPRQREYPTLKSSLNIVRETALIAISCVFWIYCLVVLLVYIG
                     TIFEIHDESINTIRVALNIENTEILDIFETMGIFAIIIFVFFTISILIQKWQRGRES"
     gene            2367..>2671
                     /locus_tag="ACGEXS_002529"
     CDS             2367..>2671
                     /locus_tag="ACGEXS_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intercellular adhesin biosynthesis
                     polysaccharide N-deacetylase"
                     /protein_id="HFF8013483.1"
                     /translation="MKYRKFIILVLSILIILPVSTLDGHHIANADDDSPKKLKYKENS
                     ALALNYHRVRKANFLNNFIYFFSSSKEIKNYSVSQSQFESQIKWLKSHDAKFLTLKE"
BASE COUNT          920 a          399 c          408 g          944 t
ORIGIN      
        1 catttaattt ataaaattca tatacatctt aatattctca atatcgattt gtattgtcaa
       61 ctttatatag atttaaaaaa ataatctcat gtcttttttt acaaaagtaa gttaattatt
      121 acaaactagt aacaaaaatt atttcttcaa aaatatattt agtagcgaat acacttcatc
      181 tttgaattga cttttacttt cttccactgc tccaaatttt tgcgaaaagg atgctttcaa
      241 ataccaactt tcaagaaaca gcaatattaa attctgaaag tcttcttttg tcatctttat
      301 ctttgattca tcatagaatt ttgctatctc tttacttaat gattgattta aatcttgtat
      361 ttgtccgtaa atatttccag aaaattcctc aggcgtatta gataattgaa cgtacattct
      421 aatatacctt tcttcgatgt cgaaaataaa ctcaaataag aattgatata aagcatcaat
      481 tgaatagttc gatttatttt gattcatcat aataatatta ttaaggtaat caaaacaaca
      541 tttaacactt tgttcgtaaa tacttttttt cgagtcaaaa tggtaatata aactcgcttt
      601 ctttatattt acacttttag ctatatcatc aagtgttgta ccgtcatacc ccttctctga
      661 aaataaggtt attgcgttat caataatctt atccttcaat ttttataacc ccctactgaa
      721 aattaatcac actatgttac aggaaaatta agttgcaatt acaaatattt ccgtttaatt
      781 ataacaacaa tctattgcaa attaaaatac tatcaattac catatggctt acaacctaac
      841 taacgaaagg taggtaaaga aattgcaatt ttttaacttt ttgctttttt atcctgtatt
      901 tatgtctatt tactggattg tcggttcaat ttatttctat tttaccagag aaattagata
      961 ttcattgaac aagaagcctg acataaatgt ggatgaatta gaaggcatta catttttact
     1021 tgcctgttat aacgaaagtg aaacgattga agatacgttg tctaatgttc ttgcactcaa
     1081 atacgagaag aaagaaatta ttatcattaa tgatggaagt tcagataata cagcagaact
     1141 catctataaa atcaaagaaa ataatgactt tattttcgtc gatttacaag aaaacagagg
     1201 taaagccaac gcactcaatc aaggcattaa acaggcttca tatgattatg taatgtgctt
     1261 ggatgcagat actatcgttg atcaagatgc accatattat atgattgaga atttcaaaca
     1321 tgatccaaaa cttggtgcag ttacaggtaa tcctagaatt cgaaataaga gttctatttt
     1381 aggtaaaatt caaacgatag aatatgcaag tttaattggc tgtattaagc gaagtcagac
     1441 acttgctggc gcagtcaata ctatttcggg tgtcttcact ctatttaaaa aaagtgcagt
     1501 tgtcgacgtt ggctactggg atactgatat gattaccgaa gatattgcag tttcttggaa
     1561 attgcattta cgtggatatc gtattaagta tgaaccgctt gccatgtgtt ggatgttggt
     1621 tccagaaaca ttgggaggtc tttggaagca acgcgtgaga tgggctcaag ggggacacga
     1681 agtattacta cgagactttt ttagcacaat gaaaacgaaa aggtttcctt tatatatttt
     1741 gatgtttgag caaatcatct cgattttatg ggtatatata gtgcttctat atttaggcta
     1801 tttgttcata acagcaaact tcttagacta tacatttatg acatatagtt tttcaatatt
     1861 tctactatca tcatttacta tgacttttat aaacgttatt caatttacag tcgcactctt
     1921 tattgatagt cgctacgaga aaaagaatat ggctggactc atatttgtaa gttggtatcc
     1981 gacagtatac tggattatta acgcagcagt agttcttgtc gcatttccaa aagcattaaa
     2041 acgtaagaaa ggtggttacg caacatggtc aagcccagac agagggaata cccaacgcta
     2101 aaatcatcgc taaatattgt aagagaaaca gcacttatcg ctatatcttg tgtcttttgg
     2161 atatattgtt tagttgttct actcgtttat attggtacta tatttgaaat tcatgacgaa
     2221 agtatcaata caatacgtgt tgctttaaac attgaaaata ctgaaatttt agatatattt
     2281 gaaactatgg gcattttcgc gattatcatt tttgtatttt ttacaattag catattgatt
     2341 caaaaatggc agagaggaag agaatcgtga agtatagaaa atttataatt ttagtgttga
     2401 gtatcttgat catattgcct gtaagcacac tggatggtca tcatattgca aatgcagatg
     2461 acgattcacc taaaaaactg aaatataaag aaaatagtgc tctggcatta aattatcacc
     2521 gtgtaagaaa agcgaatttt ctgaataatt ttatttactt cttttctagt agtaaagaaa
     2581 ttaaaaatta tagtgttagt caatcacaat ttgaatctca aataaaatgg ctaaaatcac
     2641 atgatgctaa atttttaacc ttgaaagaat t
//
LOCUS       DAXWFK010000226         2651 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.228, whole genome shotgun sequence.
ACCESSION   DAXWFK010000226 DAXWFK010000000
VERSION     DAXWFK010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.228"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..969)
                     /locus_tag="ACGEXS_002530"
     CDS             complement(<1..969)
                     /locus_tag="ACGEXS_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HFF8013484.1"
                     /translation="MLVALNEEKERVLATTALRKTQYFCPVCGKQVILKRGLKVISHF
                     AHKHLAEQKCFNNETIKHYKSKLILAQMIQQQGCKVEIEPFLKEIKQIPDILINNKYV
                     IELQYSPIPYKQILQRTEGLKKMGYKVSWLLNDVDYCHNKVKFNHFQSMFINPFTRKL
                     HTFNLEKKQIMMFQQIQYLGGHKYVAEKRNAKISELFNEAPCDYHAVYKLSKFAINQY
                     IKYCRWQNSVLEPTLSAMYQLQLTDQEVVHNYGYIFPEQIYIKNHPIEWQLQVDLWLK
                     NGKSKLVNDNLNYFKLKKFIVALESKTAIIEKLINNYLNICSDKGND"
     gene            complement(1090..1809)
                     /gene="mecA"
                     /locus_tag="ACGEXS_002531"
     CDS             complement(1090..1809)
                     /gene="mecA"
                     /locus_tag="ACGEXS_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFF8013485.1"
                     /translation="MRIERVDDTTVKLFITYSDIEARGFSREDLWTNRKRGEEFFWSM
                     MDEINEEEDFVVEGPLWIQVHAFEKGVEVTISKSKNEDMMNMSDDDATDQFDEQVQEL
                     LAQTLEGEDQLEELFEQRTKEKEAQGSKRQKSSARKNTRTIIVKFNDLEDVINYAYHS
                     NPITTEFEDLLYMVDGTYYYAVYFDSHVDQEVINDSYSQLLEFAYPTDRTEVYLNDYA
                     KIIMSHNVTAQVRRYFPETTE"
     gene            complement(2180..2575)
                     /gene="spxA"
                     /locus_tag="ACGEXS_002532"
     CDS             complement(2180..2575)
                     /gene="spxA"
                     /locus_tag="ACGEXS_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFF8013486.1"
                     /translation="MVTLFTSPSCTSCRKAKAWLQEHDIPYTERNIFSEHLTIDEIKQ
                     ILKMTEDGTDEIISTRSKTYQKLNVDIDSLPLQDLYSIIQDNPGLLRRPIILDNKRLQ
                     VGYNEDEIRRFLPRKVRTFQLQEAQRMVD"
BASE COUNT          867 a          445 c          330 g         1009 t
ORIGIN      
        1 gtcattacct ttatctgaac aaatatttaa ataattgtta ataagttttt ctataattgc
       61 tgttttactt tctagagcaa caataaattt tttcagttta aaataattaa gattgtcatt
      121 tactaatttg ctttttccat tctttaacca taaatcaact tgtaattgcc actcaatagg
      181 atgattttta atataaattt gctctggaaa aatataacca taattgtgca ctacttcttg
      241 atcagttaac tgtaattgat acattgcact taaagtgggt tctaaaacag aattttgcca
      301 gcgacaatat ttgatatatt gattaattgc gaactttgat aatttataaa cagcatgata
      361 atcacaaggc gcctcattaa acaactcact aattttggca tttctttttt cagcgacata
      421 tttgtgcccg cctaaatatt gtatttgttg aaacatcatt atttgttttt tctctaaatt
      481 gaacgtatga agttttcgag tgaatggatt aataaacata ctttgaaaat gattgaactt
      541 cactttatta tgacaataat caacatcatt taataaccaa cttactttat atcccatttt
      601 ctttaaacct tccgttcgtt gaagaatctg tttataagga attggcgaat actgtagctc
      661 aataacatat ttattattaa tcaaaatatc cggaatttgt tttatttctt ttaaaaatgg
      721 ctctatctct actttacatc cttgttgctg tatcatctgt gctaaaatca atttactttt
      781 ataatgttta atcgtttcat tattaaaaca tttttgttcc gctaaatgtt tatgtgcaaa
      841 atgactaatt actttgagcc cacgctttaa aataacttgc ttgccacaca ccggacaaaa
      901 atattgtgtc tttctcaatg cagtagttgc taatacgcgt tccttttctt catttaaagc
      961 tactaacata tcttcacctc aaaatattac acgtaagttt aactactatt actttttcag
     1021 cttttgaaat caataaaaaa agagactcca aagagtctcc tttcatagtg cgttgcttaa
     1081 ttaatatcgt tattcagttg tctctggaaa ataacgtcga acttgagctg ttacgttatg
     1141 actcataatt attttagcat agtcatttaa ataaacttct gttctgtctg ttggataagc
     1201 aaattcaagc aattgactgt aactatcatt aatgacttct tgatcaacat gactatcaaa
     1261 atatacagca taataataag taccatcaac catatataac aaatcttcaa actctgtagt
     1321 tattggattg ctatgatatg cataattaat aacatcttct aaatcgttaa atttcacaat
     1381 gattgttctt gtatttttac gtgctgaaga cttttgacgt ttagaacctt gagcttcttt
     1441 ttcttttgtt cgttgctcga ataattcttc taattgatct tcaccttcta atgtttgagc
     1501 taacaattct tgaacttgtt catcaaattg atcagttgca tcatcatcag acatattcat
     1561 catatcttca tttttagatt tagaaattgt gacttcgaca cctttttcaa aggcatgtac
     1621 ttgaatccat aatggacctt ctacaacaaa atcttcttct tcgttaattt catccatcat
     1681 tgaccaaaag aattcttcgc cacgtttgcg atttgtccat aaatcttcac gactaaatcc
     1741 acgggcctcg atatcgctat atgttataaa caattttaca gttgtatcat ctactcgttc
     1801 tattctcata tcatctcact ccttacagtc gatgaatagt atccacattg tattaaagca
     1861 tcaaacaaaa tacaatttat ttgtttgatt tctttctcta aaatattttg gtaacttata
     1921 ataacatatt cgttgtagca aaactattta aatgatttat acagttaaag cattaaagca
     1981 ctttcttaat ttaattttac cttatcatag caaatgtttc atccaaaaca ctctaatata
     2041 gagctatatt aacaattgca catttgattt aaaagatact aagcaattat tgtaaaatta
     2101 aaaccaaaaa agcaacacta tagaaatagt gttgcttgac atatctttta gtaaagtatg
     2161 acacatagtt aaatggttat tagtcaacca tacgttgtgc ttcttgtaat tggaacgtac
     2221 gaacttttct aggtaagaaa cgtcgaatct cgtcctcatt ataaccaact tgtagtcgtt
     2281 tattatctaa aataattgga cgacgtaata agccaggatt atcttgaatg attgaatata
     2341 agtcttgtaa tggtagtgaa tcaatatcaa catttaattt ttggtatgtt ttagaacgtg
     2401 tagaaatgat ttcatcagta ccgtcttcag tcatttttaa tatttgctta atttcatcaa
     2461 ttgttaaatg ttcagaaaaa atattacgct ccgtatacgg aatgtcatgt tcttgtaacc
     2521 atgctttcgc tttacggcaa gatgtgcaac ttggtgaagt aaataatgtt accatacatc
     2581 tcactctcct atttgaatga ataaaattca ttgcttaaaa tttagttata gatcaagaaa
     2641 aaacattttt t
//
LOCUS       DAXWFK010000227         2638 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.229, whole genome shotgun sequence.
ACCESSION   DAXWFK010000227 DAXWFK010000000
VERSION     DAXWFK010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.229"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(90..1133)
                     /gene="recA"
                     /locus_tag="ACGEXS_002533"
     CDS             complement(90..1133)
                     /gene="recA"
                     /locus_tag="ACGEXS_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFF8013487.1"
                     /translation="MDNDRQKALDTVIKNMEKSFGKGAVMKLGDNIGRRVSTTSTGSV
                     TLDNALGVGGYPKGRIIEIYGPESSGKTTVALHAIAEVQSNGGVAAFIDAEHALDPEY
                     AQALGVDIDNLYLSQPDHGEQGLEIAEAFVRSGAVDIVVVDSVAALTPKAEIEGEMGD
                     THVGLQARLMSQALRKLSGAISKSNTTAIFINQIREKVGVMFGNPETTPGGRALKFYS
                     SVRLEVRRAEQLKQGQEIVGNRTKIKVVKNKVAPPFRVAEVDIMYGQGISKEGELIDL
                     GVENDIVDKSGAWYSYNGERMGQGKENVKMYLKENPQIKEEIDRKLREKLGISDGDVE
                     ETEDAPKSLFDEE"
     gene            complement(1298..2449)
                     /locus_tag="ACGEXS_002534"
     CDS             complement(1298..2449)
                     /locus_tag="ACGEXS_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HFF8013488.1"
                     /translation="MSIAIIAVGSELLLGQIANTNGQFLSKVFNEIGQNVLEHKVIGD
                     NKKRLESSVRHALEKYDTVILTGGLGPTKDDLTKHTVAQIVGKDLVIDEPSLKYIESY
                     FEEQGQEMTPNNKQQALVIEGSTVLTNHHGMAPGMMVNFENKQIILLPGPPKEMQPMV
                     KNELLSHFINHNRIIHSELLRFAGIGESKVETILIDLIDKQTNPTIAPLAGSHEVYIR
                     LTANADSKEQAQSLIQPVKQEILDRIGEYYYGSDDTLIEQAVIKKIHEPFVIYDGITN
                     GALYHRLKEVDLNDVLKGMINHNENFVDINKPIEQQLKDAVQFVNKLFNVSSAIILLE
                     YDGVVHIGYDNNFEFKTEQFKMSKSRNLLKNRSQNYALIRLLNWLRTTN"
BASE COUNT          816 a          505 c          347 g          970 t
ORIGIN      
        1 tattattcta gctttatgag atgaatatca ctttcaatca atcctataag gatatttgct
       61 aagtttaact atagatataa atttgtgtac tattcttcgt caaataatga ctttggtgca
      121 tcttctgttt cttcaacatc accatcagat atacctaatt tttctctcaa tttacgatca
      181 atttcttctt taatttgtgg attttctttc aagtacattt taacattttc cttaccttga
      241 cccattcgtt cgccattgta agaataccat gctcctgatt tatcaacgat gtcgttttca
      301 acacctaaat caataagttc accctcttta gaaatacctt gtccatacat aatatcaact
      361 tcagctactc taaatggtgg tgccacttta tttttaacga ctttaatttt agttctatta
      421 cctacaattt cttgtccttg tttaagctgt tctgcacgac gtacttctag tcttactgaa
      481 ctatagaatt ttaatgcacg tccacctggt gtagtctctg gattaccgaa cataacacca
      541 actttttcac gaatttggtt gatgaaaata gcagttgtat ttgatttaga aatagcacct
      601 gaaagtttac gtaacgcttg tgacattaaa cgagcttgta aaccaacgtg agtgtctccc
      661 atttctcctt caatttcagc tttaggtgtt aaagcagcaa ctgagtctac aactacaata
      721 tcaactgcac cacttctaac aaatgcttcg gcgatttcaa gaccttgttc accatgatcc
      781 ggttgcgata aatataaatt atcgatatct acgcctaatg cttgagcata ttctggatct
      841 aaagcatgtt cagcatcgat aaatgctgcc accccgccat tactttgtac ttcagcaata
      901 gcgtgaagcg ctactgttgt cttaccagaa ctttcaggac cataaatttc aataattcgt
      961 cctttaggat agccacctac acctagcgca ttatctaatg taactgaacc agttgatgta
     1021 gttgaaactc ggcgacctat attgtcaccc aacttcatta cggcaccttt accgaaagat
     1081 ttctccatat ttttaattac tgtatctaaa gctttttgac gatcgttatc caaagcgaga
     1141 cctcctaatt gaaattgcta tcttttaatt atcttaccaa tactatacaa ggattcaaaa
     1201 taaaatacaa gtgttttgcg aatatttgtt cgtataaaat cttgaaatca aaacgcaaaa
     1261 acgaacaaac gtgcttatat ttttatcgat aatacaatta atttgttgtt ctaagccaat
     1321 ttaataatct tatgagcgca taattttgac ttctgttctt taataaattt ctagatttag
     1381 acattttaaa ttgctcagtt ttaaattcaa agttattatc atagcctata tgaactacac
     1441 catcatactc taatagaata attgctgatg acacattaaa caatttatta acaaattgca
     1501 ctgcatcttt taattgctgc tcaataggtt tattaatatc aacaaagttt tcattgtgat
     1561 taatcatacc ctttagaaca tcgtttaaat ccacttcttt caatcgatga tataaagcac
     1621 cattagtaat accatcatat attacaaaag gttcatgaat tttctttatt acagcttgct
     1681 caattaatgt gtcatctgaa ccataataat attctccaat acgatcaaga atttcttgtt
     1741 taacaggttg aatcaatgat tgtgcttgtt cttttgagtc ggcatttgca gtcaatctaa
     1801 tatatacttc atgacttccc gccaaaggcg caatcgtagg attagtctgt ttatcgataa
     1861 gatctattaa tattgtttct actttagatt cacctattcc cgcaaatctt aatagttcag
     1921 aatgtataat tcgattatgg tttataaaat gtgacaacaa ttcatttttc accattggtt
     1981 gcatttcttt cggtggacct ggtaataaaa taatttgttt gttttcaaaa ttcaccatca
     2041 ttcctggagc catgccatga tgatttgtta atacagttga accttcaatt actaaagcct
     2101 gttgtttatt attaggtgtc atttcttgtc cttgttcctc aaaatagctt tcaatatatt
     2161 ttaaagaagg ctcatcaata actaaatctt taccaacaat ctgggccact gtatgcttcg
     2221 ttaagtcatc tttcgtagga cctaagccac ctgttaaaat aacagtatca tatttttcta
     2281 gcgcatgacg tacacttgat tctaaacgtt ttttattatc tccaataact ttatgttcta
     2341 atacattttg tccaatttca ttaaatactt tagatagaaa ttgtccgttg gtattagcga
     2401 tttgacctag caatagttct gagcctacag caataatggc aattgacatt tatatccccc
     2461 ttgtatcgtt taattcattt tttcatgtat ttcatttaaa aattcacatc aattttagat
     2521 tcaatgtatc agatgctact acagattttc cctaaaaaaa tgaacaaata ttgttttaca
     2581 atatatcatt ataaacaatt gcgtggctaa tttctaaaat tgggattaaa aaaactag
//
LOCUS       DAXWFK010000228         2635 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.230, whole genome shotgun sequence.
ACCESSION   DAXWFK010000228 DAXWFK010000000
VERSION     DAXWFK010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.230"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(330..1538)
                     /gene="cozEb"
                     /locus_tag="ACGEXS_002535"
     CDS             complement(330..1538)
                     /gene="cozEb"
                     /locus_tag="ACGEXS_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell elongation protein CozEb"
                     /protein_id="HFF8013489.1"
                     /translation="MNENEKNIRKNFLKFTESRYMKFVGGNDLVFSLIALVLLGIVIF
                     IFEKVSYVFDPFIIVFKTIAAPIIVSLILFYLFNPIVNMMERYRIPRVAGISIIYLAV
                     VGVITLIVNLLIPIIGSQVDSLVKNSPQYLEKLINSIDKIANNTFFSSYYSQINDWLN
                     SLPKKIPSMLSEFTDGFGSKIATFAETIANIGVVIVTTPFVLFFMLKDGHHFKEFSTN
                     IMPPKFRKDFHDLLEKMSVQVGSYIQGQIIVSFCIGILLFIGYSVIGLKYSLVLASIA
                     AVTSVVPYLGPTIAISPAIVIAAITSPWMLLKLAVVWTLVQFVEGHFISPNIMGKTLK
                     IHPLTIIFILLCAGKLLGIVGVILGIPGYAILKVLVTHLFQLFKRRYNRFYGNDVGEY
                     DIKESNKIVE"
     gene            complement(1531..1698)
                     /locus_tag="ACGEXS_002536"
     CDS             complement(1531..1698)
                     /locus_tag="ACGEXS_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013490.1"
                     /translation="MKKGLINPKLSVLHGRFVTVKGICMVSLMLVILHKWTVFIQHLM
                     NILSGGESIIE"
     gene            complement(1673..2524)
                     /locus_tag="ACGEXS_002537"
     CDS             complement(1673..2524)
                     /locus_tag="ACGEXS_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFF8013491.1"
                     /translation="MGEYIVTKTLNNNVVVCTNNDQEVILIGKGIGFNKKEGMALNDQ
                     TITIEKIYKLESEQQKAHYKSLVEIADDNVLQVIIDSLNFISNTAMNVDSKQLVVSLT
                     DHIIFAYKRLKQNQVISNPFVMETMQLYSDAYHIAKQVIDQLNAALDVHFPEDEIGFI
                     ALHIASNTEDLSMHEMTLINNVIKKGIDIIESDLVTTVDKESLQYQRFIRHVQFLIRR
                     LRRKEYIHAQDDFVSMIKNHYPICYNTAYKILTMIQKQFDVNISESEIIYLTLHIHHF
                     EERINQS"
BASE COUNT          963 a          430 c          327 g          915 t
ORIGIN      
        1 acagaacaat atggttatct atactgattc atatttaaca taaagaaaat gatgaatcaa
       61 tacttatgaa aaatattatt aattaacagc cgattatgag tgcttcttaa tatattaaaa
      121 ataccactta tttttatttt cgacataata atgaagtact aatcaagtgg caaatactcg
      181 tttcattatg catgtgcttt ataaacggct aaactttcgt aaaaatttat aagctaaaca
      241 tgtgctgata atcataaatg taactgccca acattataag caccataaaa aataccaaca
      301 aacatatagt cattggtatt ttttattact tattcaacta ttttattact ttctttaata
      361 tcatattcac ctacatcatt accatagaaa cgattgtatc gacgtttaaa taattggaat
      421 aaatgagtaa ctaatacttt taaaatagca tatcccggaa tacctaaaat aacgcctaca
      481 ataccaagca atttgcctgc acacagtaaa atgaaaatga ttgtaagtgg atgaatctta
      541 agtgttttac ccatgatatt tggtgaaatg aagtgccctt caacaaattg tactaaagtc
      601 catactactg ctaattttaa gagcatccac ggcgatgtta tagcagctat tacaatagct
      661 ggagaaatcg ctatagtagg ccctaaatat ggtacaacac ttgtaactgc cgcaatacta
      721 gctaatacta agctatattt caacccgata accgaataac cgataaacaa cagtataccg
      781 atacagaatg aaacgataat ttgtccttga atgtatgaac caacttgaac actcattttt
      841 tcaagtagat catgaaaatc ttttcggaat ttcggtggca taatattcgt tgaaaattct
      901 ttgaagtgat gtccatcttt aagcataaag aatagtacaa atggtgttgt gacaatcaca
      961 acgccaatat tagcaatcgt ttctgcaaac gttgcaattt tagacccaaa gccatctgta
     1021 aattcactta acatagatgg tattttctta ggtaaagaat ttaaccaatc attaatttga
     1081 ctataatacg aagagaaaaa cgtattattt gctattttat caatagaatt aattaatttt
     1141 tctagatatt gcggtgaatt tttaactaaa ctatctactt gcgaaccaat aataggtatc
     1201 aataaattaa caattaacgt aataacacct actacagcta gataaataat agaaatacct
     1261 gcaactcttg gtattctata acgttccatc atatttacga ttgggttaaa tagatagaat
     1321 agaattaaag agacgatgat aggtgctgct atcgtcttaa aaacgatgat aaaaggatca
     1381 aaaacatatg atactttttc gaaaataaaa ataacaatac ccaacaatac tagcgctatt
     1441 aatgagaaga ctaaatcatt cccaccaaca aacttcatat accgtgattc ggtaaatttt
     1501 aaaaaattct ttcttatatt cttttcattt tcattcaatt atagattcac caccacttaa
     1561 aatattcata agatgttgta taaaaacagt ccacttatgt aaaattacta acatcaatga
     1621 taccatacaa atacccttta cagtaacgaa acgcccgtgt aatacactta atttaggatt
     1681 gattaatcct ttcttcaaaa tgatgaatgt gtaatgttaa atatataatt tcagactcac
     1741 tgatattaac atcaaattgt ttttgtatca tagttaaaat tttatatgct gtgttatagc
     1801 aaatcggata gtgattttta atcatagaca caaaatcatc ttgtgcatgt atatattctt
     1861 ttcttcttaa tcggcgaatt aaaaattgta cgtgccttat aaaacgttgg tattgtaatg
     1921 attccttatc aacagttgtc acaaggtctg attcaatgat atctatacct tttttaataa
     1981 cattattgat caaggtcatc tcatgcatag ataaatcttc tgtattagat gcaatatgta
     2041 atgcaataaa tcctatctca tcttcaggaa aatgtacatc taatgctgca tttaactgat
     2101 caatcacctg tttagcaata tgatatgcat cactatataa ctgcatagtt tccataacaa
     2161 atggattgct aataacttga ttttgtttta agcgtttata agcaaatata atatgatccg
     2221 ttaatgaaac tacaagttgt tttgaatcaa cattcatcgc agtattagaa ataaaattca
     2281 acgaatcaat aattacttgt aatacattat catcagcgat ttcaactaaa cttttataat
     2341 gtgctttttg ttgctcactt tctaatttat aaattttctc tattgtaata gtttggtcgt
     2401 ttaacgccat tccctctttt ttgttaaaac caataccttt accgattaaa ataacttctt
     2461 gatcattatt agtacatact acgacattgt tgttcaatgt tttagtaaca atatattctc
     2521 ccattatcat cacctatttt tctattatta agattatata tcggaaatgt ctaacttgaa
     2581 agtataataa tttaaatact tgtgttctat tacacgccta tcctatatga tatat
//
LOCUS       DAXWFK010000229         2629 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.231, whole genome shotgun sequence.
ACCESSION   DAXWFK010000229 DAXWFK010000000
VERSION     DAXWFK010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.231"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(112..810)
                     /locus_tag="ACGEXS_002538"
     CDS             complement(112..810)
                     /locus_tag="ACGEXS_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HFF8013492.1"
                     /translation="MSVGILIFVISVIISIITTMRENSHKDRQNQKPPQKTSTDNEPK
                     KGGFFEEIERTFKEISEELNEEEKKSSKRKYDDTLPPLFDELPKEEPKSKPVVEPMAP
                     KKQQETKPMTEKPITVPKAEPVEQKHRPSRQDNSDEIRRQLEKSLRDDIKTIRTDIDR
                     EKEKQIAKMEKRARDIIEDKYLSERTKRLKLKQLLNSQNVEKDLTKSAFQFDKDEVIN
                     GMIWSEILAKPKQL"
     gene            complement(827..1816)
                     /gene="floA"
                     /locus_tag="ACGEXS_002539"
     CDS             complement(827..1816)
                     /gene="floA"
                     /locus_tag="ACGEXS_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500186.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HFF8013493.1"
                     /translation="MFSLSFIVIAVIIVVALLILFSFVPIGLWISALAAGVHVGIGTL
                     VGMRLRRVSPRKVIAPLIKAHKAGLALTTNQLESHYLAGGNVDRVVDANIAAQRADID
                     LPFERAAAIDLAGRDVLEAVQMSVNPKVIETPFIAGVAMNGIEVKAKARITVRANIAR
                     LVGGAGEETIIARVGEGIVSTIGSSKHHTEVLENPDNISKTVLSKGLDSGTAFEILSI
                     DIADVDISKNIGADLQTEQALADKNIAQAKAEERRAMAVATEQEMKARVQEMHAKVVE
                     AESEVPLAMAEALRSGNISVKDYYNLKNIEADTGMRNAINKRTDQSDDESPEH"
     gene            complement(1834..2517)
                     /locus_tag="ACGEXS_002540"
     CDS             complement(1834..2517)
                     /locus_tag="ACGEXS_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFF8013494.1"
                     /translation="MTTILESMAGDTWVEQVSNIIVQPIFTLILTCLTFLGFVYQLYS
                     KKINAAGIIATLSLLILFLGFLIQGNVNMHSILIFSIGVILVVIELFVVGAVIGIIGM
                     ILITISITTLGDNLLFMLANVIVALILTIVEWVILVKIFNRKIPFLDKVILKDSTNSE
                     SGYNSHDNRSHLVGKTAQTVTDLRPAGIIFCENERIDAVSDGNFILRNKTVKILEVEG
                     TRVVVREVD"
BASE COUNT          800 a          504 c          380 g          945 t
ORIGIN      
        1 ctgtaattta aataactaat atttctcgaa aaattctctt tgttttttaa tttttcttaa
       61 cgtaaaaaaa caagcaacta ccattacgat agtgcttgtt ttcaaaaaat tttataattg
      121 ttttggttta gctaaaattt ctgaccatat cataccattg attacttcat ctttatcaaa
      181 ttggaacgct gatttagtca aatctttttc gacattttga gaattaagca gctgctttaa
      241 tttcaaacgt tttgtacgtt cagataagta tttatcctca ataatatctc tagcacgttt
      301 ttccatttta gcaatttgct tttctttttc tctatcaatg tcagtacgaa tcgttttaat
      361 atcatctcta agtgattttt ctaattgacg tctaatttcg tcagaattat cttgtctaga
      421 aggtctatgt ttctgctcca ccggttctgc tttaggcact gtgattggtt tctctgtcat
      481 cggttttgtt tcttgttgtt ttttaggtgc cataggttct acaacaggtt tcgatttagg
      541 ctcttccttt ggaagttcat cgaataaagg tggtaacgta tcatcatatt ttcgtttcga
      601 tgatttcttt tcttcttcat ttaattcttc acttatttct ttaaacgttc gctcaatttc
      661 ttcaaaaaag ccaccttttt ttggttcatt atcggtagat gttttttgag gtggcttttg
      721 attttgtcta tctttatgac tattttcgcg catagtagta atgatagaaa tgatcactga
      781 tatgacaaaa attagaatac cgacactcat ttaatcacct ctcgacttaa tgttcaggtg
      841 actcatcatc actttgatca gttcgtttat taattgcatt tctcatgcct gtatcagctt
      901 cgatattttt caaattataa taatctttaa cactgatatt acctgaacgt aatgcttcag
      961 ccatagctaa tggtacttca gattcggctt caactacttt agcatgcatt tcttgtacac
     1021 gcgctttcat ttcttgctca gttgctacag ccatagctct acgttcttca gcttttgctt
     1081 gtgcaatatt tttgtctgct aatgcttgtt cagtttgtaa gtctgcacca atatttttac
     1141 taatatcaac gtcagcaata tcaattgata aaatttcaaa tgcagtacct gaatctaaac
     1201 ctttgcttaa aactgtttta gaaatattat ctgggttttc aagtacttct gtatgatgct
     1261 tactagaacc aattgttgaa acgatacctt caccaacacg tgcgatgatt gtttcttcac
     1321 cagcaccacc aacaagtcga gcaatattag ctctaactgt gatacgagct ttggctttca
     1381 cttcaatacc gttcattgct acacctgcga taaatggtgt ttcaatgact ttaggattaa
     1441 cagacatttg aaccgcttct aatacgtcac gtcctgcaag gtcaattgca gcagcacgtt
     1501 cgaaaggaag atcaatgtca gcacgttgtg cagcaatatt agcgtcaaca actctgtcaa
     1561 catttcctcc tgctagataa tgcgattcta attggtttgt tgttaatgct agtcctgctt
     1621 tgtgcgcttt aattaatggc gctataactt ttcttggaga tacacgacgt aaacgcatac
     1681 caaccaatgt acctatacca acatgaacgc cagctgctaa cgctgaaatc cataaaccaa
     1741 tgggtacaaa tgagaataaa ataagtaatg caactactat aataactgct attacgataa
     1801 aacttaaact aaacatggta tcgctccttt taattaatct acttccctca caactactct
     1861 tgttccttca acttcaagga tttttaccgt tttattgcgc aaaataaagt tgccatctga
     1921 aacagcatca atacgttcat tttcacaaaa aataatccct gcaggtcgaa gatctgtaac
     1981 tgtttgagca gtctttccta cgaggtgcga gcggttatca tgagaattgt aacctgactc
     2041 agaattagtt gaatctttta agataacttt atccaaaaac ggaatctttc tgttgaaaat
     2101 cttcactaat atcacccatt ctacaatcgt taaaatcaag gcaacgataa cattcgcaag
     2161 cataaatagc aaattatcac cgagcgttgt tatgcttata gttatcagta tcatgccaat
     2221 aataccaatt actgcaccaa ctacaaataa ttcaattaca actaatataa cgccaattga
     2281 gaatattaag atagaatgca tattgacatt tccttggatt agaaatccca aaaataaaat
     2341 aagtaatgat aatgtggcga taataccagc tgcattgatt tttttagagt aaagttgata
     2401 tacaaatcct aagaatgtca aacaggttaa tattaacgta aaaataggtt gaacaattat
     2461 attgctaact tgttcaaccc aagtatctcc agccattgat tccaagatag ttgtcatttg
     2521 taaaaaatta ttataactca caatttcacc tcgccctcac attaattata catgaaattg
     2581 aatatacttt atagttataa gaacctatag aatatcaatt tgattattt
//
LOCUS       DAXWFK010000230         2569 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.232, whole genome shotgun sequence.
ACCESSION   DAXWFK010000230 DAXWFK010000000
VERSION     DAXWFK010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.232"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            39..431
                     /locus_tag="ACGEXS_002541"
     CDS             39..431
                     /locus_tag="ACGEXS_002541"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HFF8013495.1"
                     /translation="MKKVINVVGAIIFSDNKILCAQRSEKMSLPLMWEFPGGKVEKNE
                     TEKDALIREIREEMKCDLIVGDKVITTEHEYDFGIVRLTTYKCTLNKELPTLTEHKSI
                     EWLSINELDKLNWAPADIPAVNKIMTEG"
     gene            433..>2569
                     /locus_tag="ACGEXS_002542"
     CDS             433..>2569
                     /locus_tag="ACGEXS_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFF8013496.1"
                     /translation="MSRLLNDFNQSLHKGFIDKHISHKGNYTPKLLVNNKNEKVLSTI
                     IDELQKCETFYFSVAFITESGLASLKAQLLDLSNKGVKGKILTSNYLGFNSPKMYGEL
                     LKLKNVEVRLTDIAGFHAKGYIFEHKDYSSMVIGSSNLTSNALKVNYEHNVLLSTMKN
                     GDLVDSVKSEFDLLWQKSTPLTEQWINSYKESFEYRSLEKLAEVEQTQMLLADKVKKS
                     VEIVPNLMQAEALRSLKAIRDKAKDKALIISATGTGKTILCALDVREVNPNKFLFIVH
                     NEGILNRAKEEFKKVLPIKNDSDFGLLTGKHRDVDAKYLFATIQTLSRDDNFKQFDEN
                     EFDYIVFDEAHRSAASTYQRVFNYFKPKFMLGMTATPERSDELSIFELFDYNIAYEIR
                     LQAALESDILCPFHYFGVTDYVHQGIKEDDVTKLRYLTSDERVNYIIQKTDYYGYSGE
                     ILQGLIFVSSKKEAYDLADKLSSKGIKSVALTGDDSVNYRQIVIEKLKEGKINYIITV
                     DLFNEGIDIPEVNQVVMLRPTESSIIFIQQLGRGLRKSSNKEYVTVIDFIGNYKTNYL
                     IPIALSGDQSQNKDNYKKFLTNNDSINGVSTINFEEVAKKQIYNSLDAVSLNQNKLIL
                     KAYEEVENRLGHMPLLMDFIQQHSIDPSVIFSKFSNYYEFLVRYKKIDTLLTENESKN
                     LVFFSRQIAPGLKRIDSLVLEELLKNELTY"
BASE COUNT          997 a          281 c          465 g          826 t
ORIGIN      
        1 taacaattaa gatgatttta aattgaaggg tgagtcttat gaaaaaagta atcaatgtag
       61 taggagctat tattttttct gataacaaaa ttctttgtgc acagagaagt gaaaaaatga
      121 gtctgccttt aatgtgggaa tttcctggcg gtaaggttga aaagaatgaa actgaaaaag
      181 acgctttgat tagagaaatt agagaagaaa tgaaatgtga tttaattgtt ggagacaaag
      241 ttataactac agaacatgaa tatgattttg gaattgttag gttaacaaca tacaaatgta
      301 ctttaaacaa agagttacca actctaactg aacataagag tattgaatgg ttgtcaataa
      361 acgaacttga taaattaaat tgggccccag cggatatacc agccgttaat aaaattatga
      421 ctgagggata atatgagtag attactaaat gatttcaatc aatctttaca taaagggttt
      481 atagacaaac atatttctca taaagggaat tatacaccga agcttttagt aaataataaa
      541 aatgaaaaag ttctttcaac aattatagat gaacttcaaa aatgtgaaac gttttatttt
      601 tctgtggctt ttataacaga aagcggttta gccagcttaa aggctcaact attagattta
      661 agcaataagg gtgttaaagg gaaaatatta acatctaatt acttagggtt taatagtcct
      721 aaaatgtatg gagaattact taaattaaaa aatgtagagg taagattaac tgatattgct
      781 ggattccatg ccaaaggata tatttttgag cataaggatt atagttctat ggtaatagga
      841 agctctaatt taacatctaa tgcgttaaag gttaattatg agcataatgt tttactgtct
      901 actatgaaaa atggcgattt agttgatagt gtaaaaagtg aatttgactt gttatggcaa
      961 aagagtaccc cactgactga gcaatggatt aattcatata aagaatcatt tgagtaccgt
     1021 tccttagaaa aattagcgga ggttgaacaa actcaaatgc tgctagctga taaggtgaaa
     1081 aaatcagtgg aaattgtacc caatttaatg caagcagaag cattaaggtc attaaaagct
     1141 attagggata aagccaaaga taaggcgtta ataatatctg caactggtac aggtaaaacg
     1201 attttatgtg cattagatgt tagagaagta aaccctaata aatttttatt tattgttcat
     1261 aatgagggga ttttaaatag agctaaggaa gaatttaaaa aagtattacc tataaaaaat
     1321 gatagtgatt ttggattgtt gacaggaaaa catagagatg tcgatgccaa atatttattt
     1381 gcaaccattc aaacattatc tagagatgat aattttaagc aatttgatga aaatgaattt
     1441 gattatattg tttttgatga agcacatcgt tctgctgcat ctacttatca acgagtattt
     1501 aactacttca aacctaagtt catgctggga atgactgcta caccagaaag atcagatgaa
     1561 ttaagtatat ttgaactgtt tgattataat attgcatatg aaataaggtt acaagcagca
     1621 ttagaaagtg atattttatg tccatttcat tattttggtg tgactgatta tgtacatcaa
     1681 ggtattaaag aagatgatgt aactaaactg agatatttaa cttctgatga aagagttaat
     1741 tatattattc aaaagacaga ttactatgga tattcaggtg aaattttaca aggattgatt
     1801 tttgtaagta gcaaaaaaga agcttatgat ttagcggata aactaagttc aaaagggatt
     1861 aaaagtgttg ctttaacagg agatgatagt gttaattatc gccaaattgt aatagaaaaa
     1921 ttaaaagaag gtaagattaa ctatattata actgtagatc tttttaacga aggtattgat
     1981 attccagaag tcaatcaagt tgtaatgttg agaccaacag agtctagtat tatattcatt
     2041 cagcaacttg gtagaggatt aagaaaaagt tcaaataaag agtatgttac agtaattgat
     2101 tttattggta attataagac aaattattta attccgattg cgctttctgg ggatcaatcg
     2161 caaaataaag ataattataa gaagttttta acgaataacg attcgattaa tggagtatct
     2221 acaattaatt ttgaagaagt tgctaaaaaa cagatttaca attcattgga tgcagtatca
     2281 ttaaatcaaa ataaattaat attaaaagct tatgaagaag ttgaaaatag attgggacac
     2341 atgccgttac taatggattt catacaacaa cattctatag atccaagcgt tatattttct
     2401 aaatttagta attattacga gttcttagtg agatataaaa aaatagatac attattgacg
     2461 gaaaatgaat ctaaaaatct ggttttcttt tcaaggcaaa tagcacctgg attgaaaaga
     2521 atcgatagtt tagttttaga agaactttta aaaaatgaat taacatatg
//
LOCUS       DAXWFK010000231         2558 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.233, whole genome shotgun sequence.
ACCESSION   DAXWFK010000231 DAXWFK010000000
VERSION     DAXWFK010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.233"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            72..1037
                     /locus_tag="ACGEXS_002543"
     CDS             72..1037
                     /locus_tag="ACGEXS_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFF8013497.1"
                     /translation="MKKIMITGALGQIGTELVVKCREIYGTDNVLATDIREPEADSPV
                     QNGPFEILDVTDRDRMFELVRDFEADSLMHMAALLSATAEKNPILAWDLNMGGLMNAL
                     EAARTYNLHFFTPSSIGAFGDSTPKVNTPQVTIQQPTTMYGVNKVAGELLCQYYFKRF
                     GVDTRSVRFPGLISHVKEPGGGTTDYAVEIYFKAVREGHYTSFIDKGTYMDMMYMDDA
                     IEAIIKLMEADDAKLETRNGYNLSAMSFDPEMVKEAIQEYYPNFTLDYDVDPIRQGIA
                     NSWPDSIDTSCSRGEWGFDPKYDLASMTKLMLEAIEQKDTVKNNN"
     gene            1372..2448
                     /locus_tag="ACGEXS_002544"
     CDS             1372..2448
                     /locus_tag="ACGEXS_002544"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFF8013498.1"
                     /translation="MSQAVKVERRETLKQKPNTSQLGFGKYFTDYMLSYDYDADKGWH
                     DLKIVPYGPIEISPAAQGVHYGQSVFEGLKAYKRDGEVALFRPEENFKRLNNSLARLE
                     MPQVDEAELLEGLKQLVDIERDWIPEGEGQSLYIRPFVFATEGALGVGASHQYKLLII
                     LSPSGAYYGGETLKPTKIYVEDEYVRAVRGGVGFAKVAGNYAASLLAQTNANKLGYDQ
                     VLWLDGVEQKYIEEVGSMNIFFVENGKVITPELNGSILPGITRKSIIELAKNLGYEVE
                     ERRVSIDELFESYDKGELTEVFGSGTAAVISPVGTLRYEDREIVINNNETGEITQKLY
                     DVYTGIQNGTLEDKNGWRVVVPKY"
BASE COUNT          905 a          337 c          507 g          809 t
ORIGIN      
        1 ttatgaaaat gaatattatg tccgcttgtg atggactata gctatataga agagacaaag
       61 gagatatttc tatgaaaaaa attatgatta ctggtgcatt aggacaaatt ggtacagaat
      121 tagttgttaa gtgcagagaa atttatggga cagataatgt tcttgctaca gatattaggg
      181 aacctgaagc agactcacct gtacaaaatg gaccatttga aatcttagac gtaacagatc
      241 gtgaccgtat gtttgagtta gttagggact ttgaagcgga tagtctaatg catatggcag
      301 cattattatc agcaactgct gagaaaaatc caattctagc ttgggattta aatatgggtg
      361 gattaatgaa tgcattagaa gctgcaagaa cttataattt gcactttttc acaccaagtt
      421 caattggtgc atttggagac tcaactccta aagttaatac gccacaagta acgattcagc
      481 aacctacgac aatgtatggt gtaaataaag tagctggaga attattgtgt caatactatt
      541 tcaaacgttt tggtgtagat acaagaagtg ttagattccc aggtttaatc tcgcatgtta
      601 aagagccagg tggcggtact acagactatg ctgttgaaat atacttcaaa gcagtaagag
      661 agggtcatta tacaagcttc atagataaag gcacgtatat ggatatgatg tatatggatg
      721 atgcaattga agcaattatt aaacttatgg aagcagacga cgctaaatta gaaactagaa
      781 atggttataa tttgagcgca atgagttttg atccagagat ggtaaaagaa gcaattcaag
      841 aatactatcc caattttaca ttagattacg atgttgatcc tattagacaa ggtatcgcta
      901 atagttggcc ggattctatt gatacaagct gttcacgtgg cgaatgggga tttgatccta
      961 aatatgattt agcgagcatg actaaattaa tgttagaagc tattgaacaa aaagatactg
     1021 ttaaaaataa taactaatca tttccattca ctttaataca cggaatgata ttttaaatta
     1081 ctctttattt taataaacta gtgcatgaat tctaatatta ttcattatac ttattgaatt
     1141 cgcgagctta gttcatttta aagtaaagag ttttttgtat gtaaagtata ttagtaaaac
     1201 acagattact tgtctctgtg aaattaatac gtttatacta aaagatatag tctttctaga
     1261 aatctgttaa ttaattttga atttttagaa aatttgttga acagcaaaat atggattgtt
     1321 ataatttaag ttaaacaaat tcttatacaa tattattagg aggcaatcac catgtcacaa
     1381 gcagttaaag ttgaacgacg agaaacatta aaacaaaaac caaatacatc tcaactaggt
     1441 tttggtaaat attttactga ttatatgttg agttatgatt atgatgcaga taaaggatgg
     1501 catgatttga agatagtacc ttatggtcct attgaaattt cacctgctgc acaaggtgtt
     1561 cattatggtc aatcggtatt cgaaggatta aaagcatata aaagagatgg ggaagttgca
     1621 cttttccgtc ctgaagaaaa ttttaagcgt cttaataact cgttagcacg attagaaatg
     1681 cctcaagtag acgaagcaga attgttagag gggctaaaac aattagttga tattgaaaga
     1741 gattggattc ctgaagggga aggtcaatca ttatatattc gtccatttgt ttttgcaaca
     1801 gaaggggcac ttggcgttgg tgcatcacat cagtataaat tattaattat tttatctcct
     1861 tcaggtgcat attatggtgg tgaaacttta aaaccaacta aaatctatgt agaagatgaa
     1921 tatgtgcgtg ctgttcgtgg cggtgtaggc tttgcaaaag ttgcaggtaa ctatgcggca
     1981 agtttattag cacaaactaa tgcaaataaa ttaggttatg accaagtatt atggcttgat
     2041 ggtgttgaac agaaatatat cgaagaagtt ggtagcatga acattttctt cgttgaaaat
     2101 ggcaaagtaa ttacaccaga gttgaatggc agtattttac ctggtattac acgtaaatct
     2161 attatcgaat tagctaaaaa cttaggatat gaagtcgaag agcgccgcgt ttcaatcgat
     2221 gaattattcg aatcatatga taaaggtgag ttaacagaag tatttggtag tggtactgca
     2281 gcagttattt cacctgtggg tacattgaga tacgaagatc gtgaaatcgt tattaataat
     2341 aatgagactg gtgaaattac tcaaaaatta tacgacgtct atactggtat tcaaaatggt
     2401 actttagaag ataaaaatgg ttggagagtc gttgtaccaa aatattaata aaaattgaat
     2461 ataatcatga aaacaatatg caaaagttaa tcatgactaa aatccctttc aatgaagcga
     2521 agcatggtaa taaattaagt ttacaatgtt tattgtta
//
LOCUS       DAXWFK010000232         2503 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.234, whole genome shotgun sequence.
ACCESSION   DAXWFK010000232 DAXWFK010000000
VERSION     DAXWFK010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.234"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            58..813
                     /gene="sep"
                     /locus_tag="ACGEXS_002545"
     CDS             58..813
                     /gene="sep"
                     /locus_tag="ACGEXS_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type P"
                     /protein_id="HFF8013499.1"
                     /translation="MLLSFIALTLITSPFVNCSEKSEEINGKDLQKKSELQGTALSNL
                     RQTYYHNGSAIIENKESNDQFLKNTILFNDFFTGHQWYNDLLVDLGSKDTANIYKGKK
                     VDLYGVYYGYQCTGGTPFKTACMYGGVTLHDNNQLEEEKKVPINLWIDGKQNTVPLGT
                     VKTNKKEVTVQELDLQSRHYLHETYNLYNTDAFNGKIQRGLIEFHPSSGDSVGYDLFG
                     AQGQYPDTQLRIYRDNKTIKSKNMHIDIYLYTT"
     gene            1058..1192
                     /gene="pepG1"
                     /locus_tag="ACGEXS_002546"
     CDS             1058..1192
                     /gene="pepG1"
                     /locus_tag="ACGEXS_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin PepG1"
                     /protein_id="HFF8013500.1"
                     /translation="MVALLKSLERRRLMITISTMLQFGLFLIALIGLVIKLIELSNKK
                     "
     gene            complement(1245..1352)
                     /locus_tag="ACGEXS_002547"
     CDS             complement(1245..1352)
                     /locus_tag="ACGEXS_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013501.1"
                     /translation="MIFILWCVNLYIEKGQHAQTCYPSEPVKKTVAILD"
     gene            1404..1658
                     /locus_tag="ACGEXS_002548"
     CDS             1404..1658
                     /locus_tag="ACGEXS_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFF8013502.1"
                     /translation="MINWKIRMKQKSFWVAILSAIFLFAQNIAKAIGYDIQVYTEQLT
                     DGLNAILGFLVLTGVIQDPTTKGIGDSHQALEYEEPRRKY"
     gene            1670..2425
                     /locus_tag="ACGEXS_002549"
     CDS             1670..2425
                     /locus_tag="ACGEXS_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HFF8013503.1"
                     /translation="MKTYSEARARLRWYQGRYIDFDGWYGYQCADLAVDYIYWLLEIR
                     MWGNAKDAINNDFKNMATVYENTPSFVPQIGDVAVFTKGIYKQYGHIGLVFNGGNTNQ
                     FLILEQNYDGNANTPAKLRWDNYYGCTHFIRPKYKSEGLMNKITNKVKPPAQKAVGKS
                     ASKITVGSKAPYNLKWSKGAYFNAKIDGLGATSATRYGDNRTNYRFDVGQAVYAPGTL
                     IYVFEIIDGWCRIYWNNHNEWIWHERLIVKEVF"
BASE COUNT          909 a          309 c          475 g          810 t
ORIGIN      
        1 taacaagttt atatggaatt atgctttaga ggtgagtaaa ataaaaaaac aacatttata
       61 ctactttcat ttattgccct aacgttgata acaagtccat ttgtaaattg tagcgagaaa
      121 agcgaagaaa taaatggaaa agatttgcaa aaaaagtctg aattgcaggg aactgcttta
      181 agcaatctta gacaaaccta ttatcataat ggaagtgcta taattgaaaa taaagagagt
      241 aatgatcaat ttttaaagaa cactatattg tttaatgatt tttttacagg tcatcaatgg
      301 tataatgatt tattagtaga ccttggttca aaagacaccg ccaatatata caaagggaaa
      361 aaagtggatt tatatggtgt ttattatggt tatcaatgta ctgggggtac accattcaaa
      421 acagcttgta tgtatggcgg tgtaacgtta catgataata atcaattgga ggaagaaaag
      481 aaagtaccga ttaacttatg gatagatggt aaacaaaata cagtaccttt gggaacggtt
      541 aaaactaata aaaaagaagt aactgttcag gagctagacc ttcagtcaag acattattta
      601 catgaaacat ataatttata taacacagat gcatttaatg gaaaaataca aagaggatta
      661 attgagtttc acccttcttc tggtgattcg gttggttatg atttatttgg tgctcaagga
      721 caatatccag atacacagtt gaggatatat agagataata aaacgattaa gtctaaaaat
      781 atgcatattg atatatattt atatacaact tgagtgaaat tttggctgaa aaaatttaaa
      841 atctataaaa agtaccataa gttattttat tactatactt aaaacattca cttttttact
      901 gctgtttgta agttttataa aaaatttcta tagatagttt tgaacaaagc gagaataatc
      961 tgaaagtata caagcagtaa aaaaagtata tgtgctataa tatgctttga gcaagttgga
     1021 tagatggtgg ctatctgagt ataaggaggt ggtgcctatg gtggcattac tgaaatcttt
     1081 agaaaggaga cgcctaatga ttacaattag taccatgttg cagtttggtt tattccttat
     1141 tgcattgata ggtctagtaa tcaagcttat tgaattaagc aataaaaaat aaccatcgct
     1201 aactttggct ggtttcgatg gttaaatggt tattaattta atctttaatc taaaatagcc
     1261 accgtctttt taacgggctc actagggtaa catgtttgcg catgttgccc tttttctata
     1321 tataaattaa cacaccataa tataaatatc aaatagacgg cttattggtc gtctttttat
     1381 tttggataaa aggagataag aatatgatta attggaaaat tagaatgaaa caaaaatcat
     1441 tttgggtagc gatattgtca gctatctttt tatttgctca aaacatcgcc aaagctattg
     1501 ggtatgatat tcaagtttat acagagcaat taacagacgg tttaaacgct atattaggat
     1561 ttttagtatt aactggtgtg attcaagacc cgactactaa aggtataggt gatagccacc
     1621 aagctttaga atatgaagaa ccaagaagaa aatactagga ggtaaaataa tgaaaacata
     1681 cagtgaagca agagcaaggt tacgttggta tcaaggtaga tatattgatt ttgacggttg
     1741 gtatggttac caatgtgcag atttagcagt tgattacatt tattggttgt tagaaattag
     1801 aatgtgggga aatgcaaaag atgcaatcaa taacgatttt aaaaacatgg caacagtata
     1861 tgaaaacaca ccatcgtttg ttccacaaat aggtgatgtg gctgtattta ccaaaggaat
     1921 atataaacaa tacggtcata ttggtttagt gtttaatggt ggtaatacaa atcaattttt
     1981 aattttggaa cagaactatg acggtaacgc aaatacgcct gcaaagttac gttgggataa
     2041 ttattacggc tgtactcact ttattagacc taagtataaa agtgagggct taatgaataa
     2101 gatcacaaat aaagttaaac cacctgctca aaaagcagtc ggtaaatctg caagtaaaat
     2161 aacagttgga agtaaagcgc cttataacct taaatggtca aaaggtgctt attttaatgc
     2221 gaaaatcgac ggcttaggtg ctacttcagc cactagatac ggtgataatc gtactaacta
     2281 tagattcgat gttggacagg ctgtatacgc gcctggaaca ttaatatatg tgtttgaaat
     2341 tatagatggt tggtgtcgca tttattggaa caatcataat gagtggatat ggcatgagag
     2401 attgattgtg aaagaagtgt tttaattctt aggttaaaat gttaaatatt tgttaattat
     2461 ttttaatgta agtttagttt cttttaatat tttattgatt ttt
//
LOCUS       DAXWFK010000233         2442 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.235, whole genome shotgun sequence.
ACCESSION   DAXWFK010000233 DAXWFK010000000
VERSION     DAXWFK010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.235"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..128
                     /locus_tag="ACGEXS_002550"
     CDS             <1..128
                     /locus_tag="ACGEXS_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013504.1"
                     /translation="GYFIASIISPLNLNKLNNLYVEEEFYHTYNELIVKYTEVKE"
     gene            134..1615
                     /locus_tag="ACGEXS_002551"
     CDS             134..1615
                     /locus_tag="ACGEXS_002551"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFF8013505.1"
                     /translation="MKKIFMMVHELDVNKGGMTSSMFNRSKEFYDADIPADIVTFDYK
                     GNYDEIIKALKKQGKMDRRTKMYNVFEYFKQISNNKHFKSNKLLYKHISERLKNTIEI
                     EESKGISRYFDITTGTYIAYIRKSKSEKVIDFFKDNKRIERFSFIDNKVHMKETFNVD
                     NKVCYQVFYDEKGYPYISRNINANNGAVGKTYVLVNKKEFKNNLALCVYYLEKLIKDS
                     KDSIMICDGPGSFPKMFNTNHKNAQKYGVIHVNHHENFDDTGAFKKSEKYIIENANKI
                     NGVIVLTEAQRLDILNQFDVENIFTISNFVKIHNAPKHFQTEKIVGHISRMVPTKRID
                     LLIEVAELVVKKDNAVKFHIYGEGSVKDKIAKMIEDKNLERNVFLKGYTTTPQKCLED
                     FKLVVSTSQYEGQGLSMIEAMISKRPVVAFDIKYGPSDFIEDNKNGYLIENHNINDMA
                     DKILQLVNNDVLAAEFGSKARENIIEKYSTESILEKWLNLFNS"
     gene            complement(1704..2060)
                     /locus_tag="ACGEXS_002552"
     CDS             complement(1704..2060)
                     /locus_tag="ACGEXS_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFF8013506.1"
                     /translation="MKLGMLLIRWMLATSFLVHGTLKFVDLDGTIHFLGSLGLPPTIA
                     VLMSIGEVVGGLALIIGFLTPYVNVFYICVMLGSIFTLKLTRGYVSGYEFEIIHIVMN
                     LACISSYSWKKWLQFY"
BASE COUNT          986 a          273 c          393 g          790 t
ORIGIN      
        1 atggatattt tattgcgtca attataagcc cattaaattt aaataaactt aataatttat
       61 atgttgaaga agaattttat catacatata acgaattaat tgttaaatat actgaggtaa
      121 aggaataatt ataatgaaaa aaatatttat gatggtacat gagttagatg tgaataaagg
      181 tggtatgacc tcttcgatgt tcaatagaag taaagagttt tatgatgcgg acatacctgc
      241 tgatattgtt actttcgatt acaaaggaaa ctatgatgaa attattaaag ctttgaaaaa
      301 acaaggtaaa atggatcgaa gaacgaaaat gtataatgta tttgagtatt ttaaacaaat
      361 ttcaaataat aaacatttta agtctaataa attgttatat aaacatattt cagaaagact
      421 aaaaaatacg attgaaattg aagagagtaa aggtatttca agatattttg atataacgac
      481 tggtacatat attgcctaca ttagaaaaag taaatctgaa aaagtgattg atttctttaa
      541 agataataaa cgaattgaac ggtttagttt tatagataat aaagtgcata tgaaggaaac
      601 atttaatgta gataataaag tttgttatca agtattttat gatgaaaagg gatacccata
      661 tatttcaagg aatattaatg ctaataatgg tgctgtaggt aaaacttatg tgttagttaa
      721 taaaaaagaa tttaaaaaca atttagcact gtgtgtttac tatttagaaa aactaataaa
      781 agattctaaa gatagtatta tgatttgtga tggaccaggg agttttccaa aaatgtttaa
      841 tacaaatcat aaaaatgctc agaaatatgg cgttattcat gttaatcatc atgaaaattt
      901 cgatgatacg ggtgcattta aaaaaagtga gaaatatatt attgagaatg cgaataaaat
      961 taacggtgta attgtattaa cagaggcaca aagattagat attcttaatc aatttgatgt
     1021 agaaaatatt ttcactatta gcaattttgt taagatacat aatgctccaa aacattttca
     1081 aactgaaaaa atcgtaggtc atatttctag aatggtacca acgaagcgaa ttgatttgct
     1141 tattgaagtg gctgagttag tcgtaaaaaa agataatgct gttaaatttc atatatatgg
     1201 agaaggatct gtcaaagata aaatagctaa aatgattgaa gataaaaatt tagaaagaaa
     1261 tgtttttctt aaaggatata caacaactcc acaaaaatgc ttggaagatt ttaaattagt
     1321 cgtttctaca tctcaatatg aaggtcaagg gttaagtatg atagaagcaa tgatttctaa
     1381 aaggcctgtt gttgcctttg acatcaaata cggaccaagt gattttatag aagataataa
     1441 aaatggttat ttaatagaaa accataatat taacgacatg gctgataaaa tacttcagct
     1501 tgttaataat gatgtattag cagcggagtt tggttcgaaa gcgagagaaa acattataga
     1561 aaaatattca acggaatcaa tattagaaaa atggttaaat cttttcaata gctaaatgaa
     1621 aaaatcctag gtattcatga gtacctagga tttttacgca tgataaatac aaaaaaagag
     1681 aggatagtcc tctcaaacaa tagttaataa aattgtagcc atttcttcca gctgtaacta
     1741 ctaatacaag ctaagttcat tacgatatgt atgatttcaa attcataacc acttacatat
     1801 cctcttgtaa gtttaagtgt gaagattgag cctaacataa cacaaatata aaaaacatta
     1861 acataaggcg ttaaaaagcc gataataagt gctaaacctc caacaacttc accaattgac
     1921 atcaatactg cgatggtagg tggtaaacct aaagagccta agaagtgaat tgttccatct
     1981 aaatcaacaa atttcaaagt accatgcact aagaatgatg ttgctaacat ccatctaatt
     2041 aaaagcatac ctaatttcat catgtcacct ccaacattca tatttttaaa acatagtcca
     2101 aattatattt gtaaacatgt ggaaagtaaa gattttttat aagattaatt attaaatatt
     2161 tattaaatat tctattgtat tgaacaattg ttgtagaaag atgtttaagt catcagttac
     2221 tttttgtatt tataaataaa gattagtaca gcatagtgca aagattatag ggaaatgaat
     2281 taactattgc atcgctattt atgaatcgta aacactatta tgaaattgcg agagtagtat
     2341 ggacaattag aatgatggtt tgagataaat gagtgttatt aaccagtgcg tcattttcaa
     2401 aattagatgc catttgctgt ttataataag aagatttgat aa
//
LOCUS       DAXWFK010000234         2438 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.236, whole genome shotgun sequence.
ACCESSION   DAXWFK010000234 DAXWFK010000000
VERSION     DAXWFK010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.236"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..335)
                     /locus_tag="ACGEXS_002553"
     CDS             complement(<1..335)
                     /locus_tag="ACGEXS_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001147166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFF8013507.1"
                     /translation="MKHFAQLTLPIETAVRIPQNDISRYVNEIVKKIADSEFDEFRHH
                     RGATSYHLKMMLKITSYSYTQSEFSGRRIEKLLHNSIRMMWLAQDQTPSYKTINLFRV
                     NPNTDALIES"
     gene            complement(532..1224)
                     /locus_tag="ACGEXS_002554"
     CDS             complement(532..1224)
                     /locus_tag="ACGEXS_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8013508.1"
                     /translation="MNKAERQNLIITAIQQNKKMTALELAKYCNVSKRTILRDIDDLE
                     NQGVKIYAHYGKNGGYQIQQAQSKIALNLSETQLSALFLVLNESQSYSTLPYKSEINA
                     IIKQCLSLPQTRLRKLLKRMDFYIKFDDTQHMTLPMLFSDILIYCTERNVMLVDHRVD
                     DNIKAENVIFIGLLCKHGHWHAVIYDIAQDKTAELEIENIIDISYSFGKTIQTRDISI
                     DNYHQFLNPIDS"
     gene            1609..2406
                     /locus_tag="ACGEXS_002555"
     CDS             1609..2406
                     /locus_tag="ACGEXS_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HFF8013509.1"
                     /translation="MTDKTLQQIDKLICSWLKQIDNVIPQLIMEMTTETKRHRFDLVT
                     NVDKQIQQQFQQFLATHFPEHQLLAEEKSNEMITNEINHLWIMDPIDGTANLVKQQED
                     YCIILAYFYEGKPMLSYVYDYPHKKLYKAIRGEGAFCNGIKMEEPPSLKLEDAIISFN
                     AQVMNLDTVQDLFDASFSYRLVGACGLDSMRVAKGQFGAHINTNPKPWDIAAQFLFAE
                     LLNLKMTTLDGKAIDHLKGAPFIISNKACHETVLKILNANGGYQKYR"
BASE COUNT          838 a          340 c          434 g          826 t
ORIGIN      
        1 gattcaatta gcgcatcagt attaggattc actctaaaaa gattaatagt tttataagaa
       61 ggtgtttgat cttgagctaa ccacatcatt cgaatactgt tatgaagtaa tttttctatt
      121 ctacggccag aaaattcaga ttgagtatat gaatatgagg tgatttttaa catcattttt
      181 agatgatagg atgttgcgcc acgatgatgt ctgaattcat cgaattcgct atcagctatc
      241 tttttaacaa tttcattaac atatcgcgaa atatcatttt gaggaattct aacagcagtt
      301 tctattggta gtgtaagttg ggcaaagtgt tttatttttt taagtatttc aaagtaaaat
      361 tacatgttaa tacgtagtat taatggcgag actcctgagg gagcagtgcc agtcgaagac
      421 caaggctgag acggcaccct aggaaagcga agccattcaa tacgaagtat tgtataaata
      481 gagaacagca gcaagatatt ttctaattga aaatcatctt actgctgttt tttaggaatc
      541 gatggggttt aaaaattgat gatagttatc aatggatatg tctctggttt gaatcgtctt
      601 accgaatgaa tacgaaatat ctataatatt ttcaatttcg agttcggcag ttttgtcttg
      661 agcaatgtca taaatgactg catgccaatg tccatgttta cacaaaaggc caataaatat
      721 aacgttttca gctttaatat tatcatcaac cctatgatct actaacatca catttcgttc
      781 tgtacaataa attaaaatgt cggaaaacag cattgggagt gtcatatgtt gtgtgtcatc
      841 aaatttaata taaaagtcca tgcgtttaag caattttctt aagcgtgttt gtggaagact
      901 taaacattgt tttataattg cgttgatttc gcttttatat ggtaatgtcg agtacgactg
      961 actttcatta agcactaaaa ataaggctga taattgtgtt tcagataagt ttaatgcaat
     1021 tttagattgt gcttgttgta tttggtaacc accatttttc ccataatgcg cataaatttt
     1081 aacaccttga ttttctaaat catcaatatc tcttaaaatt gtgcgtttgg atacgttgca
     1141 atatttagct aattctaaag cggtcatttt tttattttgt tgaattgcag taattattaa
     1201 attttgcctt tcagctttat tcatgacagc cacctcctaa aagatgatat attacacaaa
     1261 gtataacata agagtgacaa tgaggacatt ttcaatatct tttaaaaaaa atcgtaaaaa
     1321 ttttgtggaa aacgcttaca ttttaaaaaa gattgtgcta tgatattact tgtaacaggg
     1381 ggagcgatta aacaaagggg tagagctata taacagatag cttttaatcg ttcaagttac
     1441 atcacaacat cttcaatatt tattacttac tttcctttct atttgtcggc tagcacgtga
     1501 ctagccgatt tttttatgca taaaatgaag aagtcttcag ttgtattgtt gattctttta
     1561 atatagggta ataataaaat gataatgata tgagaagtgg tggacgaaat gacagataaa
     1621 acattacagc aaatagataa gttaatatgt tcgtggttga aacaaataga caatgttatt
     1681 ccgcaattaa ttatggaaat gactacagaa actaagcgtc atcgatttga tttagttaca
     1741 aatgtagata aacagattca acaacaattc caacaatttt tagcaacaca ttttccggaa
     1801 caccaactat tagcggaaga aaaaagtaat gaaatgatta cgaatgaaat taatcattta
     1861 tggattatgg atcccattga tggaactgcc aatttagtaa aacagcaaga agattattgt
     1921 attatattgg cgtattttta tgaaggtaaa ccaatgttat cgtacgtata tgattatccg
     1981 cataaaaagc tttataaagc aatacgagga gaaggtgctt tttgtaatgg aattaagatg
     2041 gaagaaccac catcgttaaa attggaagac gctattatat cgtttaatgc acaagtgatg
     2101 aatctagata cggtgcaaga tttatttgac gcctcattta gttatcgttt agttggtgct
     2161 tgcggattag attctatgcg agtggcaaaa ggtcaatttg gggcacatat taatacaaat
     2221 cctaaacctt gggacattgc tgcacagttt ttatttgcag aactattgaa tctaaaaatg
     2281 acgactttag atggtaaggc aattgatcat ttaaagggtg caccttttat tataagtaat
     2341 aaagcatgtc atgaaacggt acttaaaatt ttaaatgcaa atggtggtta tcaaaaatac
     2401 agatagatat tctctagaga taaaaaatga taaaaaat
//
LOCUS       DAXWFK010000235         2425 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.237, whole genome shotgun sequence.
ACCESSION   DAXWFK010000235 DAXWFK010000000
VERSION     DAXWFK010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.237"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(56..1300)
                     /gene="fabF"
                     /locus_tag="ACGEXS_002556"
     CDS             complement(56..1300)
                     /gene="fabF"
                     /locus_tag="ACGEXS_002556"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFF8013510.1"
                     /translation="MSQNKRVVITGMGALSPIGNDVKTTWENALKGVNGIDKITRIDT
                     EPYSVHLAGELKNFNIEDHIDKKEARRMDRFTQYAIVAAREAVKDAQLDINENTADRI
                     GVWIGSGIGGMETFEIAHKQLMDKGPRRVSPFFVPMLIPDMATGQVSIDLGAKGPNGA
                     TVTACATGTNSIGEAFKIVQRGDADAMITGGTEAPITHMAIAGFSASRALSTNDDIET
                     ACRPFQEGRDGFVMGEGAGILVIESLESAQARGANIYAEIVGYGTTGDAYHITAPAPE
                     GEGGSRAMQAAMDDAGIEPKDVQYLNAHGTSTPVGDLNEVKAIKNTFGEAAKHLKVSS
                     TKSMTGHLLGATGGIEAIFSALSIKDSKVAPTIHAVTPDPECDLDIVPNEAQDLDITY
                     AMSNSLGFGGHNAVLVFKKFEA"
     gene            complement(1312..2253)
                     /locus_tag="ACGEXS_002557"
     CDS             complement(1312..2253)
                     /locus_tag="ACGEXS_002557"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFF8013511.1"
                     /translation="MNVGIKGFGAYAPEKIIDNAYFEQFLDTSDEWISKMTGIKERHW
                     ADDDQDTSDLAYEASLKAIADAGIQPEDIDMIIVATATGDMPFPTVANMLQERLGTGK
                     VASMDQLAACSGFMYSMITAKQYVQSGDYHNILVVGADKLSKITDLTDRSTAVLFGDG
                     AGAVIIGEVSDGRGIISYEMGSDGTGGKHLYLDKDTGKLKMNGREVFKFAVRIMGDAS
                     TRVVEKANLTSDDIDLFIPHQANIRIMESARERLGISKDKMSVSVNKYGNTSAASIPL
                     SIDQELKNGKIKDDDTIVLVGFGGGLTWGAMTIKWGK"
BASE COUNT          718 a          519 c          360 g          828 t
ORIGIN      
        1 aagaaactga aaaaatcttc agtaactaaa acaacgttac tgaagatttt tatagttatg
       61 cttcaaattt cttgaatact aatactgcgt tatgtccacc gaatcctaag ctattactca
      121 ttgcataagt aatatcaagg tcttgcgctt catttggaac aatatccaaa tcacattctg
      181 gatctggtgt taccgcatga attgtcggtg cgactttaga gtctttaatt gaaagcgctg
      241 agaagattgc ttcaattcca cctgttgcac caagtaagtg accagtcatt gattttgttg
      301 agctaacttt taagtgttta gctgcttcac caaatgtatt tttaatagct ttaacttcat
      361 ttaagtcacc aacaggagta cttgtaccat gggcatttaa gtattgtaca tctttaggtt
      421 caataccagc atcatccata gctgcttgca ttgctctaga accaccttca ccttctggag
      481 ctggcgctgt aatatgataa gcatcacctg tagtaccata gccaactatc tcagcataaa
      541 tattggcacc tcgagcttgt gctgattcta aagattcaat tactaaaata ccagcacctt
      601 cacccataac aaaaccatct ctaccttctt ggaatggacg acatgctgtt tcaatgtcat
      661 catttgtaga aagcgctcga cttgcactga aaccagcaat tgccatatga gtaattggtg
      721 cttctgtacc accagtaatc attgcatctg catcaccgcg ttgcacaatt ttaaatgctt
      781 ctccgattga atttgtacct gttgcacatg ctgtaactgt tgcaccattt ggtccttttg
      841 cacctaagtc aattgatact tgcccagttg ccatatcagg aattaacatt ggtacgaaaa
      901 atggactcac acgtcttggg cctttatcca ttaattgttt atgtgcaatt tcaaatgttt
      961 ccataccacc gataccagaa ccaatccata caccgattcg atctgcagta ttttcattga
     1021 tatctaattg cgcatcttta acagcctctc tagctgctac aattgcatat tgagtaaatc
     1081 tatccatacg acgcgcttct tttttgtcga tatgatcttc aatattaaag tttttaagtt
     1141 ctcctgctaa gtgaacgcta taaggttcag tatcgatacg tgtaatttta tcgataccat
     1201 ttacgccttt tagagcattc tcccatgttg ttttgacatc attaccgatt ggagaaaggg
     1261 ctcccatacc tgtaataact actcttttat tttgactcat tcgttatcct cctattttcc
     1321 ccattttatt gtcattgcgc cccaagttag gccgccaccg aatccgacaa gaacaattgt
     1381 atcatcatct ttgattttac cattttttaa ttcttgatcg atacttaaag gtattgacgc
     1441 agctgaagta tttccatatt tatttacaga aacactcatt ttgtcttttg aaatacctaa
     1501 gcgttctcta gctgattcca taattctaat attagcttga tgaggaataa ataaatctat
     1561 atcatctgat gttaaattcg ctttttcaac tacacgtgtt gatgcatcac ccataattct
     1621 aacagcaaat ttaaatactt ctcgaccatt cattttcagt ttaccagtat ctttatctaa
     1681 atataaatgt ttaccacctg tgccatcaga acccatttca taacttataa tacctctgcc
     1741 atctgaaact tcaccgatga taaccgcacc tgcaccatct ccaaatagaa ctgcagtaga
     1801 acggtcagtt aaatctgtta ttttagataa tttatctgca ccgacaacta aaatgttatg
     1861 ataatctcca gattgaacat attgtttagc tgtaatcatt gaatacataa atccagaaca
     1921 tgctgcaagt tgatccatag aggcaacttt gcccgtccct aaacgttctt gcaacatatt
     1981 tgcgacagtt ggaaatggca tatctccagt tgctgtggca acaattatca tatctatatc
     2041 ttcgggctga ataccagcgt cagcgattgc ttttaaactt gcttcatatg ctaaatctga
     2101 agtatcttga tcatcatctg cccaatgtct ttctttaatt ccagtcatct tagaaatcca
     2161 ttcatcagat gtatctaaaa attgctcaaa ataggcattg tcaataatct tttctggcgc
     2221 atatgcacca aaacctttaa tacccacgtt catagttgaa caccttctta ataaaatttt
     2281 taataccagg tattaattta acatagatta ctttttaaac tcaagtcaga ggtaggaata
     2341 ttgactagtt aataaaattt taatgcttta ttatcgtcaa atttcagtat gaaattggtt
     2401 ttaatgcgct taatttagaa tagcc
//
LOCUS       DAXWFK010000236         2390 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.238, whole genome shotgun sequence.
ACCESSION   DAXWFK010000236 DAXWFK010000000
VERSION     DAXWFK010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.238"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            2..268
                     /gene="scrA"
                     /locus_tag="ACGEXS_002558"
     CDS             2..268
                     /gene="scrA"
                     /locus_tag="ACGEXS_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SaeRS system activator ScrA"
                     /protein_id="HFF8013512.1"
                     /translation="MKGSKQILLIMGIISLIVLFIFTLFIMAQYAKHYEQKSDSSNAH
                     TLNSSSAIIEQHTMSNLASLDLYAPVRNITSSRDIHPIYFMTKD"
     gene            412..720
                     /locus_tag="ACGEXS_002559"
     CDS             412..720
                     /locus_tag="ACGEXS_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013513.1"
                     /translation="MTVNIVFSIVFCISMVILGIYVAITKDFTLISFINQTAIADKHK
                     NQIAYIFTLCISLSAVFLMSSILSFEYDFIALAFLFLTIALLLIALFYVCFYKITKYP
                     "
     gene            complement(828..1061)
                     /locus_tag="ACGEXS_002560"
     CDS             complement(828..1061)
                     /locus_tag="ACGEXS_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013514.1"
                     /translation="MLRSLCKHYRILINAIKVGIEMKYKISLAYNLAIIIGSLIILCI
                     LISRGYDIYVILIPILTILASLINLFCDIKKHK"
     gene            complement(1042..2235)
                     /locus_tag="ACGEXS_002561"
     CDS             complement(1042..2235)
                     /locus_tag="ACGEXS_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8013515.1"
                     /translation="MLYLEVLKNRNFTYLLIGNFLRRSCFVLFSLQIIWFTVELTNQS
                     SLKLSMMVMSQTLPFIIFGIFGGAYSDKHNKKKILYLSDLILSLIIIIIPLLAITSNL
                     NYLTLLTISTAITIINCYTDPAFRAILPEIIDEEHLATSNALIDSLQRGSNIILPALI
                     GVIVILVGNVGVFFICSILLFLGFIFNALLKYTNNNMIDRHSKEDFSETWEFLKQSKE
                     IPFIIIIQFACILINTGLWRVVLPLFISNILKEGVGVYGLATSCLGIASLLMSLIMGL
                     LSEKRLIFKFSIGVLVWGIGLSIINVFPSVAILYIGATLLGLGQSIEGLTRSVAIQIK
                     TPNHLMGKVFSISSTSNYAADTLSLGLISIIIPLLSLSNIFILGGVIISILSLAGLKF
                     VKKSM"
BASE COUNT          896 a          403 c          285 g          806 t
ORIGIN      
        1 aatgaaaggc tctaaacaaa tacttttgat tatgggcatt atatctctta ttgttttatt
       61 tatttttaca ctattcatca tggcgcaata tgcaaaacat tatgaacaaa aatccgacag
      121 ttccaacgca catactttaa attcatcatc agccataatt gagcaacata ctatgtcgaa
      181 tttagcgtct ctagatttat acgctcctgt tcgtaacatt acttctagtc gtgatattca
      241 tcccatttat ttcatgacaa aagattaaaa cttccttttt cataacccca ttcattcaac
      301 taataaatta aaaaatgata aaaacgtact gttttttaga aacatacata tataagaaac
      361 gcatccatta ctaaaagaca gtacattata ttctatcagg aaggtgcaac aatgaccgtc
      421 aacatcgtat ttagtatcgt attctgcata agtatggtta tattaggcat ttatgtcgca
      481 ataactaaag atttcacact aatctctttt ataaatcaaa cggccattgc agataaacac
      541 aagaaccaaa ttgcatatat atttacgcta tgtataagtt tgagtgcagt atttttaatg
      601 tcttcaattc taagctttga atatgatttt attgcattag cattcttatt tttaacgata
      661 gcattattac ttatcgcatt attctacgtt tgcttttata aaattacaaa atacccataa
      721 atgtttgtga tagtgtgttg gttagattta ttgatgcgct gtcagtaagt aattgtttgc
      781 gaaaaaaata caaccttgta cgaatacaaa gttgcattaa tatagaatca tttatgcttt
      841 ttaatatcac aaaacaaatt gattaaacta gctaaaatag tcaaaattgg aatcaagatt
      901 acataaatat cataacctct agatattagt atacataata taattagcga ccctattata
      961 attgctaaat tataagccaa actaatttta tatttcattt caattcccac cttaatagca
     1021 tttattaaaa ttctataatg tttacataga cttcttaaca aactttaacc cagctaagct
     1081 aagtattgaa ataattacac ctcctaatat aaatatatta cttaagctta gtaaaggtat
     1141 aattatactt atcagtccta aagacaatgt atccgctgca taattcgatg tagatgagat
     1201 actaaatact ttccccatca aatgatttgg cgtttttatt tgaatggcaa ctgatcttgt
     1261 tagtccctct atagattgtc caagtcccaa taatgttgca cctatatata atatcgccac
     1321 acttggaaac acattaataa tcgataagcc aattccccaa actaaaacac caatactaaa
     1381 tttaaagatt aatcgctttt ctgacagcaa acccataatc aatgacatta ataaagatgc
     1441 tatacctaaa catgatgtag ctagtccata tacaccaacg ccctctttta atatattgga
     1501 aataaacaat ggtaatacaa cacgccaaag gccagtatta atcaatatgc aggcaaattg
     1561 gatgattata ataaatggaa tttctttaga ttgtttcaag aattcccaag tttcagaaaa
     1621 atcttctttt gagtgtctat caatcatgtt gttatttgta tattttaaaa gtgcattaaa
     1681 aataaatcct aaaaatagca atatactaca aataaaaaag acgccaacat taccaactag
     1741 tattacaatg acaccaatta aagcaggtaa aataatattt gagcctcttt gcaaactatc
     1801 gattaacgca ttacttgttg ctaaatgctc ctcatcaata atttcaggaa gaattgccct
     1861 aaacgcagga tccgtatagc agttaataat ggtgatagct gtagatatgg ttagaagcgt
     1921 cagataattt aaatttgatg ttattgcaag taaaggaata attattataa tcaaacttag
     1981 tataagatca gatagataaa gtattttctt tttattatgt ttatcagaat atgcgccacc
     2041 gaaaatacca aatataataa atggaagtgt ttgactcata accatcattg ataattttaa
     2101 agatgattgg tttgtcaatt caacagtaaa ccaaattatt tgtaacgaaa acagcacaaa
     2161 acaactccga cgtaagaaat taccaatcaa taaatatgta aagtttctat ttttcaaaac
     2221 ttctaaatac aacatattta tcacctctca taaaaataat tgaatgcatc caccagcttt
     2281 tttagacctt cttctaaact ctctttatcc aaagcgcaat taattctaat ataatttagt
     2341 cagttaaata tcaattattt cgaaatatac atactacttg aaacaccata
//
LOCUS       DAXWFK010000237         2387 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.239, whole genome shotgun sequence.
ACCESSION   DAXWFK010000237 DAXWFK010000000
VERSION     DAXWFK010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.239"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..2031
                     /locus_tag="ACGEXS_002562"
     CDS             <1..2031
                     /locus_tag="ACGEXS_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8013516.1"
                     /translation="SKIIEDDIYIINHLDVFSIKNNKKTIMLYLSSDWFAELGFTFFN
                     YHYTAKLIKSSYNLKCLLLKLTYRYLDNQPLNDADIRKLQDIIKIIAKEASMDKKIAQ
                     NQYRYAYYGDLRDELEYIYQNVNQRLTLKSVADKLFVSKSNLSSQFHLLMGMGFKKYI
                     DTLKIGKSIEILLTTDSTISNISEHLGFSSSSTYSKMFKSYMDITPNEYRNLSKYNKC
                     LMLKPEPLVGKMVQEVKEIILNYIEHYKNHLTDVIHIDEDKFETPKLFQTVIQINTYT
                     EMKLVFLEGIFKTLLNKNSQVVFFIMPSILKSKNTMSEEEKFTIIKTIIESDLKIAFN
                     INNIETTYFVEEAFMSVFRQISPNELSNHNNYEVHFVFDLSLMEIRTIYRMILKLHNI
                     MLNVKLGLNITCLFEKPSVFKSLVSQIKRLKFDSLIIDNANLSSPYLMGESDELLLKN
                     ILHFKNLKQVINELDIEQEKLIFLNVENHKLLNNKERDLSNSAPLIYKTLSALYHNFD
                     GFGLNIFDNHQAFNAMHLYDKNGFKTTLGLILEKFIEYVSKPKYENSYYSIFDIENYY
                     CLVIYDWRVIESETIMSNFEDSQVYINFKNNVLNDKYLIVIETLDENSGNINHLISKE
                     LRDKYEWNPSLLSKIDNYLKPAIEIKEHNFSDNSLNINVTFNALYIIKIGKK"
     gene            2177..>2387
                     /gene="sarX"
                     /locus_tag="ACGEXS_002563"
     CDS             2177..>2387
                     /gene="sarX"
                     /locus_tag="ACGEXS_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarX"
                     /protein_id="HFF8013517.1"
                     /translation="MNTEKLETLLGFYKQYKALSEYIDKKYKLSLNDLAVLDLTMKHC
                     KDEKVLMQSFLKTAMDELDLSRTKLL"
BASE COUNT          955 a          275 c          358 g          799 t
ORIGIN      
        1 agtaaaataa ttgaagatga tatatatatt attaatcatc tggatgtatt tagtattaag
       61 aataataaaa aaacgatcat gttgtatttg agtagcgatt ggtttgcgga attaggcttt
      121 actttcttta attaccacta tacagcaaag ttgattaaat catcctataa tttgaaatgt
      181 ctactattaa aattgacata tcgatacctt gataatcagc ctcttaatga cgctgatatt
      241 agaaaattac aggatattat taaaatcatt gcaaaagaag caagtatgga taaaaagatt
      301 gcacaaaatc aatatcgata tgcgtattat ggtgatttgc gtgatgagct cgaatatatt
      361 tatcaaaatg taaatcaacg attgacatta aaaagtgtcg ctgataaatt atttgtctca
      421 aagtcaaatt tgtcatcaca attccactta cttatgggca tgggttttaa aaaatatatt
      481 gatactttga aaattggtaa atcgattgaa attctactta ctactgatag tactattagc
      541 aacataagtg aacatttagg ttttagtagt agctccactt actctaaaat gtttaaaagt
      601 tatatggata taacaccgaa tgaatatcgt aatttatcaa aatataataa atgtttaatg
      661 ctaaagccag aaccactagt aggcaaaatg gtgcaagaag taaaagaaat catattgaat
      721 tatattgaac attataaaaa ccacctaact gatgttatac atattgatga agacaaattt
      781 gaaacaccta aattgtttca aacggttatt caaataaata cttatacaga aatgaaatta
      841 gttttcttag aaggaatctt taaaacctta ttgaataaga acagtcaagt tgtctttttc
      901 atcatgccat cgattctaaa aagtaaaaat accatgtccg aagaagaaaa attcacaatc
      961 attaaaacaa taattgaaag tgatctaaag atagcattta atataaataa tattgaaaca
     1021 acttattttg ttgaagaagc ttttatgagt gttttcagac aaatatctcc aaacgaatta
     1081 agtaatcata ataattacga agtgcatttt gtttttgatt tatcattgat ggaaattaga
     1141 acaatttatc gaatgatatt aaaattacat aacatcatgt tgaatgtgaa attgggattg
     1201 aacattacct gtttatttga aaaaccttca gtttttaaat cactagtatc acaaataaag
     1261 cgacttaaat tcgattcgtt aataatagat aatgcaaatt taagtagccc ttatttgatg
     1321 ggggaaagtg atgagttact attgaaaaat attttgcatt ttaaaaattt aaaacaagta
     1381 attaatgaat tggatattga acaagaaaag ctgatttttc taaatgttga aaatcataaa
     1441 ctgcttaata ataaagaacg agatctaagt aatagtgctc cattaattta taagacatta
     1501 agtgcgctgt atcacaactt tgatggcttt ggattaaaca tttttgacaa tcatcaagca
     1561 tttaatgcga tgcatctata tgacaaaaat ggatttaaaa caacactagg tcttatatta
     1621 gaaaagttta tcgaatatgt ctcgaaacca aaatacgaaa acagttatta ttctattttt
     1681 gatatagaga attattattg tcttgttatt tatgattggc gagtgataga gagcgagaca
     1741 attatgagta attttgagga tagtcaagtt tatataaatt ttaaaaacaa tgttttaaac
     1801 gataaatatc taattgtaat agaaacattg gacgaaaata gtggcaacat taatcatttg
     1861 atttctaaag aattaagaga taaatatgaa tggaacccta gtttactatc taaaattgac
     1921 aactacctta aaccagcaat agagattaaa gagcataatt ttagtgataa ttccttgaat
     1981 attaacgtta cttttaatgc gttatacata attaaaatag gaaaaaaata acaccttgat
     2041 atgtattgca aaatttaatt tgcatttgtt ggtaattttg acaacgtatt aaaaacaaat
     2101 gagaaagaca atggctgaaa aatttaagat aataatgaca gaagcattgt ctttatatat
     2161 ttgggggtgc aacattttga atactgagaa attagaaaca ttgcttggct tctataaaca
     2221 atataaagca ttatctgaat atattgataa aaaatataag ttgtcgctaa atgatttagc
     2281 agtcttagat ttaacgatga agcattgcaa agatgaaaaa gtacttatgc aatcattttt
     2341 aaaaactgca atggatgagc tagatttaag taggacaaaa ttattag
//
LOCUS       DAXWFK010000238         2374 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.240, whole genome shotgun sequence.
ACCESSION   DAXWFK010000238 DAXWFK010000000
VERSION     DAXWFK010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.240"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            35..409
                     /gene="sarA"
                     /locus_tag="ACGEXS_002564"
     CDS             35..409
                     /gene="sarA"
                     /locus_tag="ACGEXS_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="global transcriptional regulator SarA"
                     /protein_id="HFF8013518.1"
                     /translation="MAITKINDCFELLSMVTYADKLKSLIKKEFSISFEEFAVLTYIS
                     ENKEKEYYLKDIINHLNYKQPQVVKAVKILSQEDYFDKKRNEHDERTVLILVNAQQRK
                     KIESLLSRVNKRITEANNEIEL"
     gene            complement(660..1448)
                     /locus_tag="ACGEXS_002565"
     CDS             complement(660..1448)
                     /locus_tag="ACGEXS_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFF8013519.1"
                     /translation="MNKVTINPQIQLTYQIEGKGDPIILLHGLDGNLAGFEDLQHQLA
                     SSYKVLTYDLRGHGKSSKSESYDLNDHVEDLKILMEKLNIHEAHILGHDLGGVVAKLF
                     TDKYAYRVKSLTTIASKKDDLIHSFTQLLIQYQDDIAGFNKSEAYILLFSKLFRNQEK
                     TMKWYQKQRIYSIKSEDDSAVAIRSLILHKDEPMYLKKRTCVPTLLINGEHDPLIKDK
                     NHFKLEAHFLNVTKKIFEHSGHAPHIEEPEAFMNYYLKFLKSVS"
     gene            complement(1617..2333)
                     /locus_tag="ACGEXS_002566"
     CDS             complement(1617..2333)
                     /locus_tag="ACGEXS_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013520.1"
                     /translation="MRINDKILLENIEDYFNHKGLSPHLIDDIKEKVITDIKNSEKKD
                     QDYIEYKRKSPAQIILMIQRNLFALQMNPVIFFIINFILISYLYDKQYVQFQAITGMS
                     LFYCLVIFPMTIVVYLRVSQKNYLRSNKIEMIMGTIIAIISLLLIILQAFNITWGVIP
                     ITNFGHQFFFFIGIILVIAGIFYKRLEFSGIGLLFCQKTVDAMIHNPQSAQIFSLIIW
                     ILLVVLVIYFTIRLSSRTRL"
BASE COUNT          870 a          364 c          302 g          838 t
ORIGIN      
        1 cgagcaagat gcatcaaata gggaggtttt aaacatggca attacaaaaa tcaatgattg
       61 ctttgagttg ttatcaatgg tcacttatgc tgacaaatta aaaagtttaa ttaaaaagga
      121 attttcaatt agctttgaag aattcgctgt attgacatac atcagcgaaa acaaagagaa
      181 agaatactat cttaaagata ttattaatca tttaaactac aaacaaccac aagttgttaa
      241 agcagttaaa attttatctc aagaagatta cttcgataaa aaacgtaatg agcatgatga
      301 aagaactgta ttaattcttg ttaatgcaca acaacgtaaa aaaatcgaat cattattgag
      361 tcgagtaaat aaacgaatca ctgaagcaaa caacgaaatt gaactataat tttgtttagc
      421 gcaatttggt gaagtttgat agatgataca ttctattaaa cttccttttt ttatgctctt
      481 tttacctaat tgttaagagg ttttgcacta atggcactta atataaggaa gatagccgca
      541 taacgagcag tatatatcaa atacatttca aaaaaagctg atttctatca aataatgaat
      601 agtaatcagc tttttcacat tgcctaagga ctttatgccc taggtttata tatcacatat
      661 tatgatacgc tttttaaaaa ttttaaataa taattcataa atgcttctgg ttcttcaata
      721 tgcggtgcat gtcctgaatg ttcgaagatt tttttcgtaa catttaaaaa atgcgcttcc
      781 aatttaaaat gattcttatc tttaatcaaa ggatcatgtt ccccattaat taacaaagta
      841 ggtacacatg tacgtttttt taaatacata ggttcatctt tatgcaaaat taatgaacga
      901 attgccaccg cactatcatc ctcagactta atgctatata ttctttgttt ttgataccat
      961 ttcatcgtct tctcttgatt tctaaacaat ttagaaaata aaagaatata cgcttcagac
     1021 ttattaaaac ccgctatatc atcttgatat tgtattaaca attgagtaaa gctgtgtatt
     1081 aagtcatctt tcttcgatgc aatggtagtt aatgatttta cacgataagc atatttatct
     1141 gtaaataact tagcaactac cccacctaaa tcatgtccta gaatatgtgc ctcatgaata
     1201 tttaacttct ccattagaat ttttaaatcc tcaacgtgat cgtttaaatc gtatgattca
     1261 cttttagaag acttgccatg acctcttaaa tcgtaagtaa gtactttata tgatgatgct
     1321 agttgatgtt gcaaatcttc aaatccagct aaattaccat ccaatccatg aagtaatatt
     1381 ataggatccc ctttaccttc aatttgataa gttaattgga tttgaggatt aattgtgact
     1441 ttattcattg tgacacctcc ctcaataaac tatataccca accttcattc tatatacgct
     1501 tccattttat aaacaatttt tttaaattat ttataaatta ccaaaagtta ttccaacaat
     1561 aaaaaaacta ttcatctcaa ttaatacaat taatcaagtg aatagtttta tcatatttat
     1621 aatcttgtac gtgaagataa tctaattgtg aaatatataa ctagaactac taataatatc
     1681 catataatta atgaaaaaat ctgggctgat tgtggattat gaatcattgc atcgacggtt
     1741 ttttgacaaa ataataaccc gattcccgaa aactcaagtc gcttataaaa tatgccggca
     1801 attactaaaa taataccaat gaaaaagaaa aattgatgtc caaaatttgt aattggtata
     1861 acgccccaag taatattaaa tgcttgtaat ataattaata acaaggatat gatagcgata
     1921 attgtaccca taatcatttc tattttatta ctacgcaagt aattcttttg agacaccctt
     1981 aggtatacaa caatagtcat tggaaaaatc actaaacaat aaaatagact cattccagta
     2041 atagcttgaa actgaacata ctgtttatca tataaatagg atatgagaat gaagtttata
     2101 ataaagaaaa taactggatt catttgtaaa gcaaacaaat tcctttgaat cattaatatg
     2161 atttgtgctg gagattttct tttatattca atataatctt gatctttctt ttcagaattt
     2221 tttatatcag taattacttt ctctttaata tcatcaatca aatgcggtga taaaccttta
     2281 tgattaaagt aatcttctat attttctagt aaaattttat cgttaatacg cattggtagt
     2341 actcctttaa aatgaaacat ctttatttat tgta
//
LOCUS       DAXWFK010000239         2346 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.241, whole genome shotgun sequence.
ACCESSION   DAXWFK010000239 DAXWFK010000000
VERSION     DAXWFK010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.241"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            27..505
                     /locus_tag="ACGEXS_002567"
                     /pseudo
     CDS             27..505
                     /locus_tag="ACGEXS_002567"
                     /inference="COORDINATES: protein motif:HMM:NF037202.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type ISP restriction/modification enzyme"
     gene            566..1309
                     /locus_tag="ACGEXS_002568"
     CDS             566..1309
                     /locus_tag="ACGEXS_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6414 family protein"
                     /protein_id="HFF8013521.1"
                     /translation="MDLLQIEKKGNLTKTVQLMHDEKGSASGESKVNMEFGKQSAVKQ
                     IFDSLLGMSASASASAGINGNITGERVAKTILENTLIYDFLDTVEFRKKKTIIDISEG
                     FKLNIQQDTMTYFATITPIASMFEGSKHLDEEELSFDVSKMNEAIRSIKGYFELIGTR
                     GNENRVFRFNIESFKNNYRIQDLKFMNLTLYSVLVGEISKENLNYDKEFDLNQGAKPS
                     GIGKFKGIRSEATKEDKYQVYDVILAGVK"
     gene            1310..>2346
                     /locus_tag="ACGEXS_002569"
     CDS             1310..>2346
                     /locus_tag="ACGEXS_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8013522.1"
                     /translation="MEIHVYTGPLCGFNEYINSEVKEYHRFMELIREYNIIVRASDTF
                     GTHDVDGIFDTEIENLVIFSNDFASVTTHVITNFINIVTLGRNIKKIYIQNPPKRVFQ
                     SLNSFHDSEIIEINYEYTTLKKDDVINFYKEQMAGSKIIGQTDAKKQMSIDLYKHTSL
                     ENNKPTVMLYYGPSGVGKTELAKEIAKKYKGNITRIQFSMMQNDESFNYIFGDEHAKV
                     SLSKDLVDRETNVVLIDEFDKVLPIYYNVFYQMFDEGIIEDANYKVDVSNCIFILTSN
                     FNSIEEAIVNIGEPVFSRIDSCIKFLYLTQEEKQKVIENKLNEILSSLNEKEKRIVTA
                     YNLLEKYKETE"
BASE COUNT          942 a          260 c          410 g          734 t
ORIGIN      
        1 atgcaatttt gcatcataaa tattatgtga acaaatattc aagtgatttg agtaaaggtt
       61 ttccgcgaat accgatatta aaggatgtat atggatttgt tgaaattggt agggagttag
      121 tggaattaca tttaaattat gaaaaacaac tcaattggga tggtgtagaa ataatttata
      181 ataatatgaa tccgaattat aaagttgaaa aatgaagcac cctaaaaagg gcgtgttgga
      241 tacagtaata tacaataccg atattacagt tactaacatt ccaaataaag tatataatta
      301 tattgtgaat ggcaagcctg ctattgaatg gattatagat cagtacaacg taagcatcga
      361 caagaaatct ggaatattag atgatcctaa tgaatttagt gaagatccga attacatttt
      421 aaatctgctt ttatcagtta ttactgtaag tatgaaaact cttggattaa tagataaatc
      481 accagatttt gaagtattag attaaggaga ggatatattg acaaatacta tcaaaaaaat
      541 aatttatttt gatgaactat cagcaatgga tttattacag atagagaaga aaggtaacct
      601 tactaaaaca gttcagttga tgcatgatga aaaaggtagt gcaagtggtg aatctaaagt
      661 gaacatggaa tttggtaagc aaagcgctgt aaaacaaatt tttgattctt tacttgggat
      721 gtcagcgagt gcttcggcta gtgcaggtat aaatggaaat ataactggag agcgagtagc
      781 caaaacaatt ttagagaata cattaatata tgattttttg gatacagtag agtttagaaa
      841 gaaaaaaact attattgata tttcagaggg gtttaaacta aatattcaac aagatacgat
      901 gacatatttc gcgactatta cccccatagc aagtatgttt gaaggatcta aacatctaga
      961 cgaggaggag ctatcatttg atgtttcaaa aatgaatgaa gcgattagaa gtattaaagg
     1021 ctattttgaa ttaataggaa caagagggaa tgaaaacaga gtttttagat ttaatataga
     1081 atcttttaaa aataactata gaatacaaga tttaaaattt atgaatttaa cactctactc
     1141 agtacttgtt ggtgaaataa gcaaagaaaa tctaaattac gataaagagt ttgatctaaa
     1201 tcaaggtgcc aaacctagtg gaattggaaa gtttaaggga attagatcag aagcaactaa
     1261 agaagataaa tatcaagtgt atgatgttat tcttgcagga gtgaaataaa tggaaattca
     1321 tgtttatacg ggaccgcttt gtggatttaa tgaatatatt aattctgaag ttaaagaata
     1381 tcaccgcttc atggaattaa tcagagaata caatatcata gtgcgagcaa gtgatacatt
     1441 tggaactcat gatgttgatg gtatttttga tactgagata gaaaatcttg tgatattttc
     1501 aaacgacttt gcttctgtaa caactcatgt tattactaat tttataaata tcgttacttt
     1561 aggtagaaat attaagaaaa tctatattca gaatcctcca aaaagagtat ttcaatcact
     1621 aaatagtttt cacgattctg aaataataga aataaactat gaatatacaa cattaaaaaa
     1681 agatgatgta ataaattttt ataaagagca gatggctggt tctaaaataa taggtcaaac
     1741 agacgctaaa aaacaaatga gcatagattt atataaacat acttccttag aaaataataa
     1801 accgaccgta atgctttact atggtccatc aggtgttgga aaaacagaat tagccaaaga
     1861 aatagcaaaa aaatataaag gtaatatcac tagaatacaa ttttctatga tgcagaatga
     1921 tgaatctttt aattatatct ttggagatga acatgccaaa gtatcattgt caaaagattt
     1981 agtagatcgt gaaactaatg ttgttttaat tgatgaattt gataaggtat taccaatcta
     2041 ttacaatgtt ttctatcaaa tgtttgatga aggtattatt gaagatgcca attataaagt
     2101 agacgtttca aattgtattt tcattctaac atctaatttt aatagtattg aagaagcaat
     2161 agttaatatt ggagaaccgg tattttctag aatagattcc tgtattaaat ttttatattt
     2221 aacacaggaa gaaaagcaga aagtaataga aaataaatta aatgaaatac tatcatctct
     2281 aaatgaaaaa gaaaaaagaa tagttaccgc atataacttg ttagagaaat ataaagagac
     2341 agaaat
//
LOCUS       DAXWFK010000240         2323 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.242, whole genome shotgun sequence.
ACCESSION   DAXWFK010000240 DAXWFK010000000
VERSION     DAXWFK010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.242"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            5..412
                     /locus_tag="ACGEXS_002570"
     CDS             5..412
                     /locus_tag="ACGEXS_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013523.1"
                     /translation="MSSIIGKIAIWIGIVAQIYFSVVFVRMISINIAGGSDYETIFLL
                     GLILALFTVLPTIFTAIYMESYSVIGGALFIVYAIIALCLYNFLSSILWLIGGILLIW
                     NKYSKDESTDENEKVDIESTENQFESKDKITKE"
     gene            462..1088
                     /locus_tag="ACGEXS_002571"
     CDS             462..1088
                     /locus_tag="ACGEXS_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8013524.1"
                     /translation="MEKNVEKSFIKIGLYFQIAYIVLMAITLCGFVICYGLIFGLFYL
                     LSGSRADYLIVTIVISAIISIFVIILSIVPVIVLASDLFKERISKGVILIVLAIIALV
                     LCNFVSAILWFVSAISILGRKKLVAAADTTTIQKSKGNANQASHKDTCKKELDSQDMM
                     EHPEVKNPTTKNLEGFNEEIHKDEATTKVVSDNTEPPIESKDHVSKKD"
     gene            1300..2076
                     /locus_tag="ACGEXS_002572"
     CDS             1300..2076
                     /locus_tag="ACGEXS_002572"
                     /EC_number="1.1.1.304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019152 - acetoin dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0045150 - acetoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
                     /protein_id="HFF8013525.1"
                     /translation="MTNNKVALVTGGAQGIGFKIAERLVEDGFKVAVVDFNEEGAKAA
                     ALKLSSDGTKAIAIKADVSNRDDVFNAVRQTAAQFGDFHVMVNNAGLGPTTPIDTITE
                     EQFKTVYGVNVAGVLWGIQAAHEQFKKFNHGGKIINATSQAGVEGNPGLSLYCSTKFA
                     VRGLTQVAAQDLASEGITVNAFAPGIVQTPMMESIAVATAEEAGKPEAWGWEQFTSQI
                     ALGRVSQPEDVSNVVSFLAGKDSDYITGQTIIVDGGMRFR"
     gene            2220..>2323
                     /locus_tag="ACGEXS_002573"
     CDS             2220..>2323
                     /locus_tag="ACGEXS_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013526.1"
                     /translation="MHQLKALLVLTHPRYYKTSQKHHYLIYLNKNSQS"
BASE COUNT          830 a          338 c          442 g          713 t
ORIGIN      
        1 gagtatgagc tcaattatag gaaaaatagc aatttggata ggcatcgtag ctcaaatata
       61 ttttagtgtc gtttttgtta ggatgatatc tattaatatt gctggaggat ctgattacga
      121 aacaattttt ttattaggat taatattggc tcttttcact gttttaccaa ccatctttac
      181 tgcgatttat atggaaagtt actctgtaat cggaggtgca ctttttattg tttatgctat
      241 tattgcactg tgtttatata atttcctttc gtcaatttta tggctgattg gtggtatttt
      301 gctgatttgg aataaatact caaaagatga atcgacagac gaaaatgaaa aagttgatat
      361 tgaaagtaca gagaatcaat ttgaatctaa agataaaatc actaaagaat aaagagaata
      421 tttaaggtaa agtataaatt ttaaataaat ggggaataga catggaaaaa aatgtagaaa
      481 aatcattcat aaagataggt ttatattttc aaatagctta tatagtactc atggctataa
      541 ctttatgtgg gtttgtaatt tgctatggac taattttcgg ccttttctat ttattatcag
      601 gtagcagagc tgattattta atagtaacaa tagttatatc ggcaataatt tctatatttg
      661 taattatact ttcaatcgta cctgtcatcg tattggcatc tgacttattt aaagaaagga
      721 tttcaaaagg tgtcatatta attgtattgg ctattatcgc tttagtatta tgcaactttg
      781 tatctgcaat actctggttt gtttcagcca tatctatttt aggtagaaaa aaattagtag
      841 ctgcagcaga tactaccact attcaaaaaa gtaaagggaa cgcaaatcaa gcatcacata
      901 aagacacgtg taaaaaggaa cttgatagtc aagacatgat ggaacatcct gaggttaaaa
      961 atcccacgac taaaaacctt gaaggattta acgaagaaat acataaagat gaagctacaa
     1021 ctaaagttgt cagtgataac acggaaccgc ctattgaatc aaaagaccat gtctcgaaaa
     1081 aagattgatg acaaactaat cgagagactt aaaaaaataa tattcaacat aagaactttt
     1141 aaaacgacat ttaaacgcat tgccaatcac taatggtagt gcgtttaact ataccttaaa
     1201 tatctgaata ttttgttaaa tggagctacc tttgttgtac tattcaaatg aagaggagta
     1261 aaatgtaatt aaaggaaaga aatttgagga gtgatcttta tgacaaacaa caaagtagca
     1321 ttagtaactg gcggagcaca agggattggt tttaaaattg cagaacgttt agtggaagat
     1381 ggtttcaaag tagcagttgt tgatttcaat gaagaagggg caaaagcagc tgcacttaaa
     1441 ttatcaagtg atggtacaaa agctattgct atcaaagcag atgtatcaaa ccgtgatgat
     1501 gtatttaacg cagtaagaca aactgccgcg caatttggcg atttccatgt catggttaac
     1561 aatgccggcc ttggaccaac aacaccaatc gatacaatta ctgaagaaca gtttaaaaca
     1621 gtatatggcg tgaacgttgc aggtgtgcta tggggtattc aagccgcaca tgaacaattt
     1681 aaaaaattca atcatggcgg taaaattatc aatgcaacat ctcaagcagg cgttgagggt
     1741 aacccaggct tgtctttata ttgcagtaca aaattcgcag tgcgaggttt aacacaagta
     1801 gccgcacaag atttagcgtc tgaaggtatt actgtgaatg cattcgcacc tggtatcgtt
     1861 caaacaccaa tgatggaaag tatcgcagtg gcaacagccg aagaagcagg taaacctgaa
     1921 gcatggggtt gggaacaatt tacaagtcag attgctttgg gcagagtttc tcaaccagaa
     1981 gatgtttcaa atgtagtgag cttcttagct ggtaaagact ctgattacat tactggacaa
     2041 acaattattg tagatggtgg tatgagattc cgttaataat catccactaa tgataaataa
     2101 atccttattg ttaagtttaa tcacttagca gtaaggattt tttagtgcac ttagaaggga
     2161 gtgtattggt agaaaattaa taagcgaagt tcttaagtga gttatgatgt cacagtctaa
     2221 tgcatcagtt gaaagcatta ttagtattaa cacacccaag atattataaa acatcacaaa
     2281 aacaccacta tctaatttat ctcaataaaa attcacaaag tta
//
LOCUS       DAXWFK010000241         2315 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.243, whole genome shotgun sequence.
ACCESSION   DAXWFK010000241 DAXWFK010000000
VERSION     DAXWFK010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.243"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..746
                     /locus_tag="ACGEXS_002574"
     CDS             <1..746
                     /locus_tag="ACGEXS_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFF8013527.1"
                     /translation="IICTIITYLLYNNYFVAMMVYYVVISLIMLDNFKKMKNREYQKE
                     IAEKNRHINTLIAEQERHRIGQDLHDTLGHVFASLSLKSELAYKLIDADVEKVKAELL
                     AINKLSRESLNKVREIIDDVKLPSFIEEIDSIRKVLKDADIDFTFENKELAQVLSPTK
                     QSMLVMITREAINNVIKHANASKVHGKLKTVNNHKLLLMIEDDGKGIDSDCEVKSISQ
                     RVQHLNGTLAVDSTNGTKIIIEISTGGIA"
     gene            743..1345
                     /locus_tag="ACGEXS_002575"
     CDS             743..1345
                     /locus_tag="ACGEXS_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFF8013528.1"
                     /translation="MTSLIIAEDQNMLRQAMVQLIKLHGDFEILADTDNGLDAMKLIE
                     EYNPNVVILDIEMPGMTGLEVLAEIRKKHLNIKVIIVTTFKRPGYFEKAVVNDVDAYV
                     LKERSIEELVETINKVNNGEKEYSATLMTSFFVDKNPLTPKEQIVLREIGNGLSSKEI
                     SEKLFLTDGTVRNYTSVIIDKLFADNRFDAWKKANEKGWI"
     gene            complement(1458..1646)
                     /locus_tag="ACGEXS_002576"
     CDS             complement(1458..1646)
                     /locus_tag="ACGEXS_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HFF8013529.1"
                     /translation="MNISNENTVTLIAVIIAIIIGIFLQIFFKLPLIVTAVLSILLGI
                     FVGFIVYLIVSFYNKRKN"
     gene            1785..>2315
                     /locus_tag="ACGEXS_002577"
     CDS             1785..>2315
                     /locus_tag="ACGEXS_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFF8013530.1"
                     /translation="MKSNKSLAMIVVAIIIVGVLAFQFMNHTGPFKKGTNHETVQDLN
                     GKDKVHVQRVVDGDTFIANQNGKEIKVRLIGVDTPETVKPNTPVQPFGKEASNYSKKT
                     LTNQDVYLEYDKEKQDRYGRTLAYVWISKDRMYNKELVEKGLAREKYFSPNGKYRNVF
                     IEAQNKAKQQKLNIWSK"
BASE COUNT          927 a          260 c          414 g          714 t
ORIGIN      
        1 tgattatatg tacaataata acgtacttat tgtataacaa ctattttgtt gcaatgatgg
       61 tttattatgt cgttatatcg ttaataatgc tagataattt taaaaaaatg aaaaaccgtg
      121 aatatcaaaa agaaatagca gaaaaaaata gacatattaa tacattaatt gctgaacaag
      181 agcgacatag aattggtcaa gacttacatg atacgttagg gcatgtgttt gcaagtttat
      241 cattaaaatc agaattagct tataaactaa tagatgctga tgtagaaaaa gtaaaagctg
      301 aattattagc aattaataaa ttatctcgtg aatcattgaa caaagttcga gaaattattg
      361 atgatgtaaa attaccatca tttattgaag agattgatag tatacgtaaa gttttaaaag
      421 atgctgatat tgattttaca tttgaaaata aagaattagc gcaagtatta agtcctacta
      481 aacaatctat gttagttatg attacgcgtg aagcgataaa taatgttatt aaacatgcaa
      541 atgcttcaaa agttcatggt aaattaaaaa ctgtaaacaa tcataaatta ctgcttatga
      601 ttgaagatga tggcaaaggt atcgatagtg attgtgaggt gaaaagtatt tcacagcgtg
      661 tacaacattt aaatggaact ttagcagtcg actcaacaaa tggaactaaa ataatcattg
      721 aaatctcaac aggaggaata gcatgacatc tttaattatt gcagaagatc aaaatatgtt
      781 acgacaggca atggttcaat taattaaact acatggtgat tttgaaattt tagcagatac
      841 tgataatggt ctcgatgcaa tgaaacttat tgaagaatat aatcctaacg ttgttatttt
      901 agatatagaa atgccaggca tgactggact tgaagtttta gcggaaatta gaaaaaagca
      961 tttgaatatt aaagtgatta ttgtaacaac ttttaaaaga ccgggatact ttgaaaaagc
     1021 agttgtgaat gatgtggatg catatgtttt aaaagaacgt tctatagaag aattggtgga
     1081 aaccattaat aaagtaaata acggagagaa agaatatagc gccacattga tgacttcatt
     1141 ttttgtagat aaaaacccat taacgcccaa agaacaaatt gtattaaggg aaattggcaa
     1201 tggtttaagt agtaaagaaa taagtgaaaa attatttttg acagatggaa cagttagaaa
     1261 ttatacatct gttataattg ataaattatt tgcagataat cgttttgatg cttggaaaaa
     1321 ggcaaatgaa aaaggctgga tctaaataca aacaaaaaag tattgagtgt tttgatttaa
     1381 gagacttgga aagtgagtca ataatcaaaa tcttactcaa tacttttatt ttggtactcg
     1441 caatttagat ttgttaacta attttttcgc ttattataaa acgaaacaat caagtaaacg
     1501 ataaagccta caaagatacc caataaaata gatagtactg ccgtcactat taatggtaat
     1561 ttgaaaaata tttgtaggaa aataccaatg ataattgcga taattactgc aattaatgtg
     1621 actgtatttt catttgaaat gttcatttat tttcactcct taacaataac attatatcat
     1681 gctatagctt tccaaaatat tgaaatatgt agatatggct attgacgata tttcttaact
     1741 attatatgat taatcggaat gaaaaaagag aagtaggtgg caatatgaag tcaaataaat
     1801 cgcttgctat gattgtggta gccatcatta ttgtaggtgt attagcattt caatttatga
     1861 atcatacggg tcctttcaaa aaggggacga atcatgaaac tgtacaagat ttaaatggta
     1921 aagataaagt acatgttcaa agagttgtgg atggtgatac atttattgca aatcaaaatg
     1981 gtaaagaaat taaagttagg cttatagggg ttgatacgcc agaaacggtg aaaccgaata
     2041 cgcctgtaca accatttggc aaagaagcat caaattatag taagaagaca ttaacaaatc
     2101 aagatgttta tttagaatat gataaagaaa aacaagatcg ctatggtaga acattggcgt
     2161 atgtatggat aagtaaagat cgtatgtaca ataaggaatt agtggaaaag ggacttgcta
     2221 gagagaagta tttttcacca aatggcaaat atagaaatgt atttatagaa gcacaaaata
     2281 aagctaaaca acagaaatta aatatttgga gtaaa
//
LOCUS       DAXWFK010000242         2282 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.244, whole genome shotgun sequence.
ACCESSION   DAXWFK010000242 DAXWFK010000000
VERSION     DAXWFK010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.244"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            174..1733
                     /gene="rny"
                     /locus_tag="ACGEXS_002578"
     CDS             174..1733
                     /gene="rny"
                     /locus_tag="ACGEXS_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFF8013531.1"
                     /translation="MNLLNLLLILLGIILGVVGGYVVARNLLLQKQSQARQTAEDIVN
                     QAHKEADNIKKEKLLEAKEENQILREQTEAELRERRSELQRQETRLLQKEENLERKSD
                     LLDKKDEILEQKESKIEEKQQQVDAKESSVQTLIMKHEQELERISGLTQEEAINEQLQ
                     RVEEELSQDIAVLVKEKEKEAKEKVDKTAKELLATAVQRLAADHTSESTVSVVNLPND
                     EMKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARTALVNLVSDG
                     RIHPGRIEDMVEKARKEVDDIIREAGEQATFEVNAHNMHPDLVKIVGRLNYRTSYGQN
                     VLKHSIEVAHLASMLAAELGEDETLAKRAGLLHDVGKAIDHEVEGSHVEIGVELAKKY
                     GENETVINAIHSHHGDVEPTSIISILVAAADALSAARPGARKETLENYIRRLERLETL
                     SESYDGVEKAFAIQAGREIRVIVSPEEIDDLKSYRLARDIKNQIEDELQYPGHIKVTV
                     VRETRAVEYAK"
     gene            complement(2030..2245)
                     /locus_tag="ACGEXS_002579"
     CDS             complement(2030..2245)
                     /locus_tag="ACGEXS_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013532.1"
                     /translation="MSINIDPEKFAELVVMSNPSKFEDAEDIAKESLKLYINAYRLAE
                     RYSTIATNCYDTAEVIKELKKTDLQLK"
BASE COUNT          854 a          324 c          426 g          678 t
ORIGIN      
        1 aaaactttag aaaatagtag aaatagcatt caatatagtg caaaagtgca aattgataac
       61 ttgacactta tctcctataa accgtacaat taatttgtat gatttatata taatttcata
      121 aagtcatatt gaatttcata taaagagcaa accctagaaa aggaggtgtt tgtgtgaatt
      181 tattaaacct cctactcatt ttgctgggga tcattctagg agttgttgga gggtatgttg
      241 ttgcccgaaa tttgttgctt caaaagcaat cacaagctag acaaactgcc gaagatattg
      301 taaatcaagc acataaagaa gctgacaata tcaaaaaaga gaaattactt gaggcaaaag
      361 aagaaaacca aatcctaaga gaacaaactg aagcagaact acgagaaaga cgtagcgaac
      421 ttcaaagaca agaaacccga cttcttcaaa aagaagaaaa cttagagcgc aaatctgatc
      481 tattagataa aaaagatgag attttagagc aaaaagaatc aaaaattgaa gaaaaacaac
      541 aacaagtaga tgcaaaagag agtagtgttc aaacgttaat aatgaagcat gaacaagaat
      601 tagaacgcat ctccggtctc actcaagaag aagctattaa tgagcaactt caaagagtag
      661 aggaagaact gtcacaagat attgcagtac ttgttaaaga aaaagaaaaa gaagctaaag
      721 aaaaagttga taaaacagca aaagaattat tagctacagc agtacaaaga ttagcagcag
      781 atcacacaag tgaatcaacg gtatcagtag ttaacttacc taatgatgag atgaaaggtc
      841 gaatcattgg acgagaagga cgaaacatcc gcacacttga aactttaact ggcattgatt
      901 taattattga tgacacacca gaagcggtta tattatctgg ttttgatcca ataagaagag
      961 aaattgctag aacagcactt gttaacttag tatctgatgg acgtattcat ccaggtagaa
     1021 ttgaagatat ggtcgaaaaa gctagaaaag aagtagacga tattattaga gaagcaggtg
     1081 aacaagctac atttgaagtg aacgcacata atatgcatcc tgacttagta aaaattgtag
     1141 ggcgtttaaa ctatcgtacg agttacggtc aaaatgtact taaacattca attgaagttg
     1201 cgcatcttgc tagtatgtta gctgctgagc taggcgaaga tgagacatta gcgaaacgag
     1261 ctggactttt acatgatgtt ggtaaagcaa ttgatcatga agtagaaggt agtcatgttg
     1321 aaatcggtgt agaattagcg aaaaaatatg gtgaaaatga aacagttatt aatgcaatcc
     1381 attctcatca tggtgatgtt gaacctacat ctattatatc tatccttgtt gctgctgcag
     1441 atgcattgtc tgcggctcgt ccaggtgcaa gaaaagaaac attagagaat tatattcgtc
     1501 gattagaacg tttagaaacg ttatcagaaa gttatgatgg tgtagaaaaa gcatttgcga
     1561 ttcaggcagg tagagaaatc cgagtgattg tatctcctga agaaattgat gatttaaaat
     1621 cttatcgatt ggctagagat attaaaaatc agattgaaga tgaattacaa tatcctggtc
     1681 atatcaaggt gacagttgtt cgagagacta gagcagtaga atatgcgaaa taatttttgt
     1741 ctccctcaca aattagtgag ggagcttttt taagttgtag tcttaatcta gttagacagc
     1801 actttatcgg taataactat attaaacagt agttatttga aagtaagacg gaccttatat
     1861 taaataagaa gttattgctt ttaataaaaa tgttttaggc ttcgtaatta ctatatttat
     1921 attatgtaaa cctataaaga tgattgcttt tctatccaat aaaaaagaag agaagatgta
     1981 acacatcttc tcttctgcaa tattaattag gatttatttc taagttgagt tattttaatt
     2041 gtaaatctgt tttctttaat tcttttataa cttctgcagt atcataacaa tttgttgcaa
     2101 ttgttgaata tctctctgct aaacgatatg cattaatgta aagctttaaa ctttctttag
     2161 ctatatcctc tgcatcttcg aattttgatg ggttagacat aaccactaat tctgcaaatt
     2221 tttctggatc aatattaata gacatgtatt tatttacaac tcctatttat tttgatgtct
     2281 ta
//
LOCUS       DAXWFK010000243         2267 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.245, whole genome shotgun sequence.
ACCESSION   DAXWFK010000243 DAXWFK010000000
VERSION     DAXWFK010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.245"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            156..464
                     /gene="spn"
                     /locus_tag="ACGEXS_002580"
     CDS             156..464
                     /gene="spn"
                     /locus_tag="ACGEXS_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myeloperoxidase inhibitor SPIN"
                     /protein_id="HFF8013533.1"
                     /translation="MKFKKVLVATAMVGVLATGVVGYGNQADAKVYSQNGLVLHDDAN
                     FLEHELSYIDVLLDKNADQATKDNLRSYFADKGLHSIKDIINKAKQDGFDVSKYEHVK
                     "
     gene            complement(568..2076)
                     /locus_tag="ACGEXS_002581"
     CDS             complement(568..2076)
                     /locus_tag="ACGEXS_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKLRK protein"
                     /protein_id="HFF8013534.1"
                     /translation="MRENFKLRKMKVGLVSVAITMLYIMTNGQAEASENQNALISNIN
                     VDNQEKQNNVNQAVQPQNNTNETSKVPANFVKLNDIKPGDTSIQGTTLPNQFILLTID
                     KKDVSSVEDSDSSFVMSDKDGNFKYDLNGRKIVHNQEIEVSSSDPYLGDDEEDEEVEE
                     TSTEEVGAEEESTEAKATYTTPRYEKAYEIPKEQLKEKDGHHQVFIEPITEGSGIIKG
                     HTSVKGKVALSINNKFINFETNANGGPNKEEAKSGSEGIWMPIDDKGYFNFDFKTKRF
                     DDLELKKNDEISLTFAPDDEDEALKSLIFKTKVTSLEDIDKAETKYDHTKVEKVKVLK
                     DVKEDLHVDEIYGSLYHTEKGKGILDKEGTKVIKGKTKFANAVVKVDSELGEGQEFPD
                     LQVDEKGEFSFDVDHAGFRLQNGETLNFTVVDPITGELLSGNFVSKNIDIYESPEEKA
                     DREFDERMENTPAYHKLHGDKIVGYDTNGFPITWFYPLGEKKVERKAPKLEK"
     gene            complement(2098..>2267)
                     /locus_tag="ACGEXS_002582"
     CDS             complement(2098..>2267)
                     /locus_tag="ACGEXS_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="superantigen-like protein"
                     /protein_id="HFF8013535.1"
                     /translation="IDKHNLYKTEPKDSKIRITMKDGGFYTFELNKKLQTHRMGDVID
                     GRNIEKIEVNL"
BASE COUNT          689 a          402 c          306 g          870 t
ORIGIN      
        1 aaaaatcctc aagcatcgta ttaaagatta attgcattta aacaatattt gtcactttaa
       61 ctatttatta aaaattatta aatcgactta ataaacgtaa ttttcttaat attcagtgtt
      121 atgatggagt tacaattttt agaggagtga tagatatgaa atttaaaaag gttttagtag
      181 ctacggcaat ggtaggtgtt ttagcaactg gcgttgttgg atatggtaat caagcagatg
      241 cgaaagttta ttctcaaaat ggactcgtac tacatgatga tgcaaacttc ttagaacatg
      301 aattaagtta tattgatgtg ttattagata agaatgctga ccaagctaca aaagacaact
      361 taagatcata cttcgccgat aaaggactac attcaatcaa agacattatc aacaaagcta
      421 aacaagacgg ctttgatgtt tcaaaatatg aacacgtaaa ataaattcaa taatgctttg
      481 taacaaagca caaaaaaaca gctcaatgac gtagaagtca gtgattaact cactaatttc
      541 attacatcat taagctgttt tatttaatta tttttctaat tttggtgcct tacgttcaac
      601 tttcttttca cctaatggat aaaaccaggt aatcgggaat ccgttagtat cgtagccgac
      661 aattttatca ccatgtaatt tatgatatgc aggtgtgttt tccattcttt catcaaactc
      721 acgatctgct ttttcttcag gagattcata aatatctatg tttttagaaa caaaatttcc
      781 acttaataat tcacctgtaa taggatcaac tactgtgaag tttagtgttt ctccattttg
      841 taatctaaat ccagcatgat ctacatcaaa gctgaattca cctttttcat cgacttgcaa
      901 atcagggaat tcttgacctt cacctagttc agagtctacc ttcacaactg catttgcgaa
      961 tttagtctta cctttaatta ctttagtacc ttctttatca agaatacctt tacctttttc
     1021 tgtatgatat aagcttccgt aaatttcatc tacatgtaaa tcttctttaa catctttcaa
     1081 tacttttact ttttccactt tagtatggtc atatttagtt tctgctttat caatatcttc
     1141 taaactcgtt accttagttt tgaaaattaa tgacttcaat gcctcatctt cgtcatcagg
     1201 tgcaaatgtt aatgagatct catcattttt ctttaactct aaatcatcga aacgtttcgt
     1261 tttgaagtca aaattaaagt atcctttgtc atcaataggc atccagattc cttctgatcc
     1321 agatttcgct tcttctttat ttggaccacc attagcattt gtctcaaagt taataaattt
     1381 attattaata gatagagcaa ctttaccttt tacagaggta tggcctttaa taatacctga
     1441 accttcagta ataggttcga taaaaacttg gtgatgtcca tctttttctt ttagctgttc
     1501 tttcggtatt tcatacgctt tttcatatcg cggtgttgta tatgtagctt tagcttctgt
     1561 actttcttcc tcagcaccaa cttcttcagt tgaagtttct tctacttctt catcttcttc
     1621 atcgtcacct aaatagggat ctgatgaaga cacttcaatt tcttgattat gaacaatttt
     1681 gcgaccattt aagtcatact taaaattccc atctttatca gacataacaa agctgctgtc
     1741 agaatcttca actgagctca catctttttt atcaatagtt aatagtataa attgatttgg
     1801 taaagttgtt ccttgtatag aagtatcacc tggtttaata tcattcaatt tgacaaaatt
     1861 agccggtact tttgatgttt cattagtatt attttgaggc tgaacagctt gatttacatt
     1921 attctgtttt tcctgattgt ctacatttat attagagatt aaagcgtttt gattttcaga
     1981 tgcttctgct tgtccgtttg tcataatata taacattgta attgcaacag atactaaacc
     2041 gactttcatt ttacgtaact taaaattttc cctcatgata tgctccctcg aataatttta
     2101 taaattcact tcaatttttt ctatatttct gccatcaata acatcaccca tacggtgtgt
     2161 ttgtaacttt ttattcaatt caaatgtgta gaacccacca tctttcatag taattcgaat
     2221 tttactgtct ttaggttctg tcttataaag gttatgttta tcaatta
//
LOCUS       DAXWFK010000244         2197 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.246, whole genome shotgun sequence.
ACCESSION   DAXWFK010000244 DAXWFK010000000
VERSION     DAXWFK010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.246"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..230
                     /locus_tag="ACGEXS_002583"
     CDS             <1..230
                     /locus_tag="ACGEXS_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFF8013536.1"
                     /translation="DGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVRRREKIIRIFPN
                     QTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
     gene            287..679
                     /locus_tag="ACGEXS_002584"
     CDS             287..679
                     /locus_tag="ACGEXS_002584"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8013537.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPNGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            680..2119
                     /locus_tag="ACGEXS_002585"
                     /note="aph(2'')-Ia"
     CDS             680..2119
                     /locus_tag="ACGEXS_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HFF8013538.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          910 a          191 c          375 g          721 t
ORIGIN      
        1 atgatggatt cgaagacgcc tttcaatata ccgtacaagg aaattcccac aatcgactaa
       61 agagtaccaa tctaattgaa cgactgaatc aagaagtacg cagaagagaa aagattattc
      121 gcatcttccc caatcaaaca tcagccaatc gcttaattgg agccgttctt atggacctac
      181 atgatgaatg gatttattct tcaagaaaat acatcaattt tgataagtag aaatggtaaa
      241 aacattgtat agcattttac acaggagtct ggacttgact gagtttatgg aagaagtttt
      301 aattgatgat aatatggttt ttgatattga taatttaaaa ggatttctta atgataccag
      361 ttcatttggg tttatagcta aagaaaataa taaaattata ggatttgcat attgctatac
      421 acttttaaga cctaatggaa aaacaatgtt ttatttacac tcaataggaa tgttacctaa
      481 ctatcaagac aaaggttatg gttcaaaatt attatctttt attaaggaat attctaaaga
      541 gattggttgt tctgaaatgt ttttaataac tgataaaggt aatcctagag cttgccatgt
      601 atatgaaaaa ttaggtggta aaaatgatta taaagatgaa atagtatatg tatatgatta
      661 tgaaaaaggt gataaataaa tgaatatagt tgaaaatgaa atatgtataa gaactttaat
      721 agatgatgat tttcctttga tgttaaaatg gttaactgat gaaagagtat tagaatttta
      781 tggtggtaga gataaaaaat atacattaga atcattaaaa aaacattata cagagccttg
      841 ggaagatgaa gtttttagag taattattga atataacaat gttcctattg gatatggaca
      901 aatatataaa atgtatgatg agttatatac tgattatcat tatccaaaaa ctgatgagat
      961 agtctatggt atggatcaat ttataggaga gccaaattat tggagtaaag gaattggtac
     1021 aagatatatt aaattgattt ttgaattttt gaaaaaagaa agaaatgcta atgcagttat
     1081 tttagaccct cataaaaata atccaagagc aataagggca taccaaaaat ctggttttag
     1141 aattattgaa gatttgccag aacatgaatt acacgagggc aaaaaagaag attgttattt
     1201 aatggaatat agatatgatg ataatgccac aaatgttaag gcaatgaaat atttaattga
     1261 gcattacttt gataatttca aagtagatag tattgaaata atcggtagtg gttatgatag
     1321 tgtggcatat ttagttaata atgaatacat ttttaaaaca aaatttagta ctaataagaa
     1381 aaaaggttat gcaaaagaaa aagcaatata taatttttta aatacaaatt tagaaactaa
     1441 tgtaaaaatt cctaatattg aatattcgta tattagtgat gaattatcta tactaggtta
     1501 taaagaaatt aaaggaactt ttttaacacc agaaatttat tctactatgt cagaagaaga
     1561 acaaaatttg ttaaaacgag atattgccag ttttttaaga caaatgcacg gtttagatta
     1621 tacagatatt agtgaatgta ctattgataa taaacaaaat gtattagaag agtatatatt
     1681 gttgcgtgaa actatttata atgatttaac tgatatagaa aaagattata tagaaagttt
     1741 tatggaaaga ctaaatgcaa caacagtttt tgagggtaaa aagtgtttat gccataatga
     1801 ttttagttgt aatcatctat tgttagatgg caataataga ttaactggaa taattgattt
     1861 tggagattct ggaattatag atgaatattg tgattttata tacttacttg aagatagtga
     1921 agaagaaata ggaacaaatt ttggagaaga tatattaaga atgtatggaa atatagatat
     1981 tgagaaagca aaagaatatc aagatatagt tgaagaatat tatcctattg aaactattgt
     2041 ttatggaatt aaaaatatta aacaggaatt tatcgaaaat ggtagaaaag aaatttataa
     2101 aaggacttat aaagattgat tatataatat atgaaaagct attataaaag acattagtat
     2161 taaatagttt aaaaaaatga aaaataataa aggaagt
//
LOCUS       DAXWFK010000245         2167 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.247, whole genome shotgun sequence.
ACCESSION   DAXWFK010000245 DAXWFK010000000
VERSION     DAXWFK010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.247"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(311..733)
                     /locus_tag="ACGEXS_002586"
     CDS             complement(311..733)
                     /locus_tag="ACGEXS_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFF8013539.1"
                     /translation="MAIHYETKATNVGGRKGHVYTDDRALDIDIVPPAQADGKATNPE
                     QLFAAGYASCFNGAFDLILKQNKVRDAHPEVTLTVRLEDDSDSESPKLSVSIDATIKN
                     VISQEEAEKYLQMAHEFCPYSKATQGNINVDLNVNVVD"
     gene            881..1597
                     /gene="aroD"
                     /locus_tag="ACGEXS_002587"
     CDS             881..1597
                     /gene="aroD"
                     /locus_tag="ACGEXS_002587"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFF8013540.1"
                     /translation="MTHVEVVATIAPQLSIEETLIQKINHRIDAIDVLELRIDQIENV
                     TVDQVAEMITKLKVMQDSFKLLVTYRTKLQGGYGQFTNDSYLNLISDLANINGIDMID
                     IEWQADIDIEKHQRIITHLQQYNKEVVISHHNFESTPPLDELQFIFFKMQKFNPEYVK
                     LAVMPHNKNDVLNLLQAMSTFSDTMDCKVVGISMSKLGLISRTAQGVFGGALTYGCIG
                     VPQAPGQIDVTDLKAQVTLY"
     gene            1680..>2167
                     /locus_tag="ACGEXS_002588"
     CDS             1680..>2167
                     /locus_tag="ACGEXS_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HFF8013541.1"
                     /translation="MELQQAIANRRSVKKFKRDMHIDDALLYQAIEKAADAPNHGMRE
                     PWRVVHVPKDRLGDMSKDISKFAFPNELDKQQCHYDAVTKLGGMLLLILKTDPRQRQN
                     DENYFAFGAYAQNLMLLLYEAGIGTCWKSPLYIYDPKVRKTLGIKKDEVLAGFLYLTD
                     LE"
BASE COUNT          750 a          314 c          389 g          714 t
ORIGIN      
        1 tcactgattt atatgcacat tcttgtaaat ggattatcaa tttaattata tttattttta
       61 agttcaggaa attatggaat tggttattgg tcgacactgg acttttattt gtagacggta
      121 tgcgaagaag tcaagccagt attggaaccg gtcacattta tgatgactaa ggtacaatca
      181 ttttcagtta aatattgcca gaaatttcaa tcacgaatag aaaaaataac caaccaaaaa
      241 tttttggttg gcaaatttga attttatgtg aattttatta ataacgttga ataatctctt
      301 taagttaatg ctaatctaca acatttacat ttaaatcgac attaatattt ccttgagtcg
      361 cttttgaata tggacaaaat tcatgagcca tttgtaaata tttttcagct tcttcttgag
      421 atataacatt tttaattgtc gcatcaattg aaacacttaa tttaggactt tctgagtctg
      481 aatcatcttc tagtctcact gttagtgtta cttctggatg agcatcacgc actttgtttt
      541 gctttaaaat taggtcgaaa gcaccgttga agcaagatgc ataacctgct gcaaataatt
      601 gttcggggtt agtagcttta ccatctgctt gagcaggcgg aacgatatca atatctaatg
      661 ctcgatcatc agtataaaca tgtcctttac gtccgccaac attcgtcgct ttagtttcat
      721 aatgtattgc catacttata acctcctaat ttaaaataca tttatgcttt acccattcga
      781 aaatacctta atcattttca tttatagcat tgtttgattg aaggataaaa agttgttgtt
      841 tacaataaaa ataatgagta tctgaaatga gggattcact atgacacatg tggaagtagt
      901 agcgactatc gcgccacaat tatctatcga agaaacttta attcaaaaaa ttaatcatcg
      961 tattgatgca atagacgtat tagaattacg aattgatcaa attgaaaatg tcacagttga
     1021 tcaagtggca gaaatgatta caaagctgaa ggttatgcaa gattcattca aattattagt
     1081 tacgtatcgt acaaagttac aaggtggcta tgggcaattt acaaatgact cgtatcttaa
     1141 tttaatatca gacttagcaa atatcaatgg catagatatg attgatatag aatggcaagc
     1201 agatattgac attgaaaaac atcaacgaat cattacacat ttgcaacagt ataataaaga
     1261 ggtggttata tcacatcata atttcgaaag tacgcctcca ttagatgaat tgcaatttat
     1321 attttttaaa atgcaaaaat tcaacccaga atacgttaaa ttagcagtaa tgccacataa
     1381 taaaaatgat gtgttaaatt tattgcaggc aatgtctaca ttttcagata ctatggactg
     1441 caaagttgtt ggtatttcaa tgtctaaact tggactaata agtagaacgg ctcaaggcgt
     1501 ttttggtggt gcgttgactt atggttgtat cggagtacca caagctccag gacagattga
     1561 tgttactgat ttaaaagcac aagtgacttt atactaataa aatgaattag gttgcttttg
     1621 tgtaagtgct gtcttataat tgagaagtat tatcattgtt cgtggaggag cgattttaga
     1681 tggaattaca acaagcaata gctaatagaa gaagtgtgaa aaaatttaaa agagatatgc
     1741 acatagatga cgcattgcta tatcaagcaa ttgagaaagc tgctgatgct ccaaatcacg
     1801 gaatgaggga accatggaga gttgtgcatg ttccgaaaga cagattagga gatatgagta
     1861 aggatatttc taaatttgca tttcctaatg aattagataa gcaacaatgt cattatgatg
     1921 cagttacgaa actaggtggc atgttattgc ttattttaaa aacagatcca agacaacgtc
     1981 aaaatgatga aaactacttt gcatttggtg catatgcaca aaatcttatg ttgttacttt
     2041 atgaagcggg aataggtaca tgttggaaat cgccattata tatctatgat cctaaagtaa
     2101 gaaaaacact tggtataaag aaagatgaag ttcttgctgg attcttatat ttaacggatt
     2161 tagaaga
//
LOCUS       DAXWFK010000246         2123 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.248, whole genome shotgun sequence.
ACCESSION   DAXWFK010000246 DAXWFK010000000
VERSION     DAXWFK010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.248"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            23..1579
                     /locus_tag="ACGEXS_002589"
     CDS             23..1579
                     /locus_tag="ACGEXS_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFF8013542.1"
                     /translation="MENTINESEKKKRFKLKMPGAFMILFILTVVAVIATWVIPAGAY
                     SKLSYEPSSQELKIVNPHNQVKKVPGTQQELDKMGVKIKIEQFKSGAINKPVSIPNTY
                     ERLKQHPAGPEQITSSMVEGTIEAVDIMVFILVLGGLIGVVQASGSFESGLLALTKKT
                     KGHEFMLIVFVSILMIIGGTLCGIEEEAVAFYPILVPIFIALGYDSIVSVGAIFLASS
                     VGSTFSTINPFSVVIASNAAGTTFTDGLYWRIGACIVGAIFVISYLYWYCKKIKNDPK
                     ASYSYEDKDAFEQQWSVLKDDDSAHFTLRKKIILTLFVLPFPIMVWGVMTQGWWFPVM
                     ASAFLIFTIIIMFIAGTGKSGLGEKGTVDAFVNGASSLVGVSLIIGLARGINLVLNEG
                     MISDTILHFSSSLVQHMSGPLFIIVLLFIFFCLGFIVPSSSGLAVLSMPIFAPLADTV
                     GIPRFVIVTTYQFGQYAMLFLAPTGLVMATLQMLNMRYSHWFRFVWPVVAFVLIFGGG
                     VLITQVLIYS"
     gene            1887..2114
                     /locus_tag="ACGEXS_002590"
     CDS             1887..2114
                     /locus_tag="ACGEXS_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013543.1"
                     /translation="MTHLTKVLDTLTGICVVLLFSKYFVAYANMVFDWNLRWYLLENI
                     PHLPIILFILMFIFGVPSEMIKDRQRKNNGV"
BASE COUNT          666 a          292 c          419 g          746 t
ORIGIN      
        1 aaatgtcgaa ggagatgaaa ccgtggaaaa tacaattaat gaaagtgaaa agaaaaaacg
       61 atttaaatta aaaatgccag gtgcatttat gattttattc attttaacgg ttgttgcagt
      121 tatagcaaca tgggttattc ctgctggtgc atattctaaa ctttcttacg aaccttcatc
      181 ccaagaacta aagatagtta accctcataa ccaagtgaaa aaggttccgg gtacgcaaca
      241 ggaactagac aaaatggggg ttaaaattaa gattgaacaa tttaaatcag gtgcaattaa
      301 taagccggta tcaattccga atacttatga aagattaaag caacatccag ctggaccaga
      361 acaaataaca agtagcatgg ttgaaggtac gatagaagcg gtcgatatca tggtattcat
      421 tcttgtacta gggggactta ttggcgtagt tcaagccagt ggttcttttg aatcgggatt
      481 gttagcttta acgaagaaaa caaaagggca tgaatttatg ctaattgtgt ttgtatcaat
      541 actaatgatt atcggcggga cattatgtgg tattgaagaa gaagctgtag cattttatcc
      601 gattttagtc cctatattta tagcgttagg atacgattct atcgtttcag ttggtgccat
      661 attccttgcc agctctgtcg gtagtacatt ttcaactatt aacccgttct cggttgtaat
      721 tgcctctaat gccgctggta caacttttac ggatggcttg tattggagaa taggtgcttg
      781 tattgtcggt gcgatttttg ttattagtta tttatattgg tactgtaaaa aaattaaaaa
      841 cgatcctaaa gcgtcatatt cttatgaaga caaagatgct tttgaacagc aatggtctgt
      901 attaaaagat gacgatagtg cccattttac tttgcgtaag aagataatcc ttacattatt
      961 tgtactacca tttccaatta tggtatgggg agttatgacg caaggttggt ggttcccagt
     1021 tatggcttca gcatttttaa tatttacaat tataataatg tttattgctg ggacaggtaa
     1081 atctggattg ggagaaaaag gaactgtaga tgcatttgtc aatggtgcat caagtttagt
     1141 aggtgtatct ttaattattg gtttagctcg aggtattaat ttagtgttga atgaaggtat
     1201 gatttcagat acaatcttac acttttcatc atctttagtt caacatatga gtggaccatt
     1261 atttatcatc gtattactat ttattttctt ctgtttaggt tttatcgtgc catcatcttc
     1321 tggcttagcg gtattatcaa tgcctatctt tgcaccacta gctgatacag taggtatacc
     1381 aagattcgtc atcgttacga catatcaatt tggtcaatat gcgatgttat tcttagcgcc
     1441 gactggactt gttatggcca cactacaaat gttaaacatg cgatattcac attggttccg
     1501 atttgtatgg ccggtagttg cttttgtatt gattttcggt ggcggagtac taattacgca
     1561 agtactaatt tattcataat ttgaaatgct atattataaa aaatactaat ggggttttat
     1621 gcatctcgta ggtttgtaga aatactaaac taagcgaggt gcattattat ttttgattaa
     1681 gaaaataatg acggtaatga taacactagt aagtaattga tacaatgctc tatttaataa
     1741 tgatattttt aaaatttgtt tttaatgtaa tgttagatct atggtatatt atatttatcg
     1801 tggtaaatat gtatttgctg taaacagttt ttgtaacaca tgatacatat ggttgtgaaa
     1861 atagcatata taaaggatgg ctataaatga cacatttgac aaaggtttta gatacactaa
     1921 ctggaatatg cgtagtatta ttatttagta aatattttgt ggcgtatgca aatatggtgt
     1981 ttgattggaa tttaagatgg tatttgctag aaaacatacc acatttgcca attatattat
     2041 ttattctgat gtttattttc ggagtacctt ctgaaatgat aaaagatagg caaaggaaaa
     2101 ataacggtgt ttaatttatc gat
//
LOCUS       DAXWFK010000247         2100 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.249, whole genome shotgun sequence.
ACCESSION   DAXWFK010000247 DAXWFK010000000
VERSION     DAXWFK010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.249"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            304..480
                     /locus_tag="ACGEXS_002591"
                     /pseudo
     CDS             304..480
                     /locus_tag="ACGEXS_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066885.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(458..559)
                     /locus_tag="ACGEXS_002592"
     CDS             complement(458..559)
                     /locus_tag="ACGEXS_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013544.1"
                     /translation="MQGFKEKHQELKKALCQIGLMRSISEVKQLNIA"
     gene            complement(682..777)
                     /gene="pepA1"
                     /locus_tag="ACGEXS_002593"
     CDS             complement(682..777)
                     /gene="pepA1"
                     /locus_tag="ACGEXS_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin
                     PepA1"
                     /protein_id="HFF8013545.1"
                     /translation="MMLIFVHIIAPVISGCAIAFFSYWLSRRNTK"
     gene            980..1123
                     /locus_tag="ACGEXS_002594"
     CDS             980..1123
                     /locus_tag="ACGEXS_002594"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFF8013546.1"
                     /translation="MTRERRSFSSEFKLQMVRLYKNGKPRNEIIREYDFTPSTFVNGG
                     YKM"
     gene            1727..>2100
                     /locus_tag="ACGEXS_002595"
     CDS             1727..>2100
                     /locus_tag="ACGEXS_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013547.1"
                     /translation="MSNQNLFDELEKKGYKLEDIFTKEEIKKYKAEDQLRAGKTQYVE
                     TGKDTATLYLSSAYTKTIAALGAGAISVISALTGGLVGAGVGGFLGSIAASNIDTSKG
                     IYIKLKTKKNAAGEYVLTGEKWG"
BASE COUNT          809 a          288 c          359 g          644 t
ORIGIN      
        1 ttcaatttga taccgatatt tcttatacag atgaattggg tagaaaattt aataaaaatc
       61 ctaagtcagt ttctgaaata aaaaaagagc aaaatacgtc caataaataa ttgttcatat
      121 tgtgatgaaa caaaaatata agtcattaga tgagtttaac tatgttataa atatttgtag
      181 tatctataaa aatctcgaca ctattaaaat gataaagtgc cgaggttttc ttacttattt
      241 agttaattca aagtttatgc cagattcata agaatttgtg acacttttaa tagtgtaacc
      301 attgattatt acaatttatc aaatggtcct ttagaaggta taaataacaa aataaaatta
      361 attaaacgta catcttttgg ttatggaagt tacaatcatt tgcgcaatcg aatattatta
      421 tgttcaaaac tttacgctcc aaaaagtaaa aaggaagtta agcaatgttt agttgcttaa
      481 cttcggatat tgaacgcatc agtccaattt gacatagagc cttttttagt tcttgatgtt
      541 tctctttaaa accttgcata ttttacaaaa agaaaaatta gcagtataat taagacaacg
      601 aaaataagta tttacttata caccaatccc ctcactattt gcggtagtga ggggattttt
      661 attggtgcgg ctatatgtca cctattttgt attgcgtcta cttagccaat aagaaaaaaa
      721 cgcaatggca cagccactga tgactggtgc tatgatgtga acgaaaataa gcatcacctt
      781 atacacctcc tctctgcgtc taaattgacg cctgagagat aggcgactct actattatat
      841 catcggcaaa tatacaagca cagtcacttg cttctgataa gttatatgat tctagctgat
      901 agattgaatc gtctacactt aattggacaa attctatgag aatagatatt gttaatttaa
      961 gaaagtaggc gattttatta tgacaagaga aagaagatca tttagttcag agtttaagtt
     1021 acaaatggtt agattatata aaaatggtaa gcctaggaat gaaattatac gcgagtatga
     1081 tttcacacct tcgacgtttg taaatggcgg ttataaaatg taggaaaatg gataaagcaa
     1141 catcaaaaca cgggtacatt caatcaccaa gataacttat cggatgaaga aaaagagctg
     1201 attaaattac gcaaagaagt tcaacattta aaaatggaga acgatatttt aaagcaagta
     1261 gcgctgatta tggggcaaaa atagaagtca ttcaaaagaa tgcacatcaa tattcagtat
     1321 cagcaatgtg taaagtcctg ataatactaa gaagtaccta ttatgattct ataaaaagaa
     1381 aagataataa aatcactaaa gatgattcaa acatagaaca tgccgtcata aatattttta
     1441 attctaatag aaaagtcttt ggtacaagac gaattaaaaa tcatttaaat gacaagggtc
     1501 tcactgtatc tggacaaaag ataggtcgat catgaaaaaa tctagtttct gtttatacga
     1561 aatctaaata caaaaatcat ctaaaagaaa ctaatgaaaa acgaattaaa aatctttatt
     1621 attagctgct ggtgtattat ttgttagtcc aatttcatta tcttataatt cagatgtagc
     1681 tcatgctgaa gataagttag accattctca agcaaaggta atatatttga gtaaccaaaa
     1741 tttatttgat gaacttgaga aaaaaggtta taaactggaa gatatattta caaaagaaga
     1801 aataaaaaaa tataaagctg aagaccaatt gagagcgggt aaaactcaat atgtagaaac
     1861 aggtaaagat actgcaacat tatatctttc ttctgcatat acaaaaacaa tagctgcttt
     1921 aggtgcgggg gctattagcg ttatttctgc actaacagga ggtttagttg gcgcaggtgt
     1981 tggtggtttc cttggatcta tagctgcatc gaatattgat actagtaagg gaatatatat
     2041 aaaattaaaa actaaaaaga atgcagccgg ggaatacgtt ctgacaggag aaaaatgggg
//
LOCUS       DAXWFK010000248         2098 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.250, whole genome shotgun sequence.
ACCESSION   DAXWFK010000248 DAXWFK010000000
VERSION     DAXWFK010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.250"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            64..255
                     /locus_tag="ACGEXS_002596"
     CDS             64..255
                     /locus_tag="ACGEXS_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013548.1"
                     /translation="MRKEIEALIFSDVSSYDIYVNTGVNQGLIGDIKDGYLTIDSMPY
                     IDAERLYHFAMERKSLVTN"
     gene            complement(394..579)
                     /locus_tag="ACGEXS_002597"
     CDS             complement(394..579)
                     /locus_tag="ACGEXS_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013549.1"
                     /translation="MLYILITALNVILNKLKMKDSNIISFIYSRHDRILYVNLVGNRF
                     KNNISMTKATSNIGRGF"
     gene            complement(515..796)
                     /locus_tag="ACGEXS_002598"
     CDS             complement(515..796)
                     /locus_tag="ACGEXS_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFF8013550.1"
                     /translation="MTYHERVLALRAESKRTAFDFRFEDLFSKEEWLSMSLAERQKAE
                     KAFRHEVKNMDDVRMPFSSVHDAQVKLYNVVYSYNGIKRNFKQVENEGF"
     gene            1085..1780
                     /locus_tag="ACGEXS_002599"
     CDS             1085..1780
                     /locus_tag="ACGEXS_002599"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFF8013551.1"
                     /translation="MTVLTDKVAVVTGAGSGIGEAIATLLHEEGAKVVLAGRNKEKLQ
                     NVANQLSQDSVKVVPTDVTNKEEVDELIKIAQQTFGGLDIVINSAGQMLSSKITDYQV
                     DEWDSMIDVNIKGTLYTAQAALPTMLEQSSGHLINIASISGFEVTKSSTIYSATKAAV
                     HTITQGLEKELAKTGVKVTSISPGMVDTAITAAYNPTDRKKLEPQDIAEAVLYALTQP
                     KHVNVNEITVRPV"
     gene            2031..>2098
                     /locus_tag="ACGEXS_002600"
     CDS             2031..>2098
                     /locus_tag="ACGEXS_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013552.1"
                     /translation="MNKKHVFIIIGVILCICIVASV"
BASE COUNT          726 a          310 c          381 g          681 t
ORIGIN      
        1 ttaataaata gagagagacg agagagttta tataaatact atataaacat tggagtgatg
       61 attatgagaa aagagattga agcgcttatt ttctcagacg taagtagcta tgatatttac
      121 gtgaacactg gtgtaaatca aggattaatt ggtgacatca aagatggtta cctaactatt
      181 gattctatgc cttacattga tgctgagcgt ttgtatcact ttgctatgga acgtaaatcg
      241 ttagtcacta actagttctt attgccaatg attactaccc ctagtcggcg gcaattgaag
      301 tgtgattgat gtaacttgcc ctcgttggtg agcaattgag ggcagacccc tttaattaag
      361 taaaccctaa ctccccacaa atctggaacg atactaaaag ccacgtccta tattggatgt
      421 ggctttagtc atacttatat tatttttaaa acgattacct acaagattta catataaaat
      481 tctatcatgt ctgctatata taaacgaaat gatattagaa tccttcattt tcaacttgtt
      541 taaaattacg tttaatgccg ttataagaat atacaacatt atataatttt acttgggcgt
      601 catggacact tgagaagggc attcttacat cgtccatatt tttaacttcg tgtcgaaatg
      661 ctttttcagc tttttgtctt tctgcaagag acatacttag ccattcttct ttgctaaata
      721 aatcttcgaa tcgaaaatca aatgcggttc ttttactttc tgctcttaat gctaaaacac
      781 gctcatgata tgtcacgttt tattcctccc tctaatcatt caatcgaatg ataaatcaca
      841 gtattaactt tattactaat ataatgtttt tacaataaag aagtgacgat ttttcaacat
      901 ttttaaaaat aaaaaatata agcaacgatt aattgtatgt ttcgaatggc ttgtaatgta
      961 atttatctat gaaaatgaag cgagttatta tgaccgttat atgaattgtg atacgctttt
     1021 taaaagtaat atgtatttat gatttaccct gaaaaaataa aaagaatcaa aggagtcgat
     1081 cattatgaca gtattaacag ataaagtagc agtagttaca ggtgcaggta gtggtattgg
     1141 agaagcaatt gcaacattac tacatgaaga aggggcaaaa gttgtcttag caggtagaaa
     1201 taaagaaaaa ttacaaaacg tagcgaatca attgtcacaa gatagtgtga aggtagtgcc
     1261 aacagatgta acgaataaag aagaagtcga tgaattgata aaaattgcac aacaaacatt
     1321 cggtggtttg gatattgtta tcaatagtgc ggggcaaatg ttgtcgtcta agattactga
     1381 ttatcaagta gatgagtggg atagtatgat tgatgtgaat atcaaaggca ctttatatac
     1441 ggcacaggct gcattaccaa ctatgttaga acaatcaagt ggccatctta ttaacattgc
     1501 atctatttct ggctttgaag taacgaaaag tagtacgatt tatagtgcga cgaaagcagc
     1561 agttcacact attactcaag gattagaaaa agagttggca aagacaggcg ttaaagtaac
     1621 aagcatttct ccaggaatgg tagatacagc cataactgcc gcatacaatc caacagatcg
     1681 taaaaaactt gaaccacaag atattgcaga agcagtatta tatgcattaa cacaaccaaa
     1741 gcacgttaac gtgaatgaaa ttacagtgcg tcctgtataa aaaggtgttt gaggtctggg
     1801 gtataggcat ttaatgtaat agctatcgat tttgttaatg ttagtaaggc aataattaca
     1861 tgagggcata ataggcaatt attccttgta caattctcag taattgctga ttttaacaat
     1921 caagttaatg aaggcgacga tgttagtgtt atggtttata atctgtaatg ttcataatta
     1981 tagattataa aaattttttc attttccacc acattttagg agttacttta atgaacaaaa
     2041 aacatgtttt tataattatt ggtgtcattt tatgtatatg tatagttgca tcagtcat
//
LOCUS       DAXWFK010000249         2081 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.251, whole genome shotgun sequence.
ACCESSION   DAXWFK010000249 DAXWFK010000000
VERSION     DAXWFK010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.251"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            43..399
                     /locus_tag="ACGEXS_002601"
     CDS             43..399
                     /locus_tag="ACGEXS_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4889 domain-containing protein"
                     /protein_id="HFF8013553.1"
                     /translation="MKKKKGFGLGISLIAIMLIVCIVLVIMMMTGGKKDTYYGIMKDN
                     TTIEKMISEKDESIEKNVKLPSDSDVKVKKGDFVIVYKLADSDKIVKVKKVDHDDVPH
                     GLMMKIHDMGKMHMKH"
     gene            complement(803..2080)
                     /locus_tag="ACGEXS_002602"
     CDS             complement(803..2080)
                     /locus_tag="ACGEXS_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HFF8013554.1"
                     /translation="MALFKRKISLPMQVVIALVLGVVVGLLLYGQENVANYIKPFGDV
                     FLNLIKMIVIPVVFCSLALSISNVGESKTVGRYGWKTILYFEIITTIAIGLGIIFGNL
                     FKPGAGLDPTKLPKGDISKYQSTAHAAEQSTYGNHFIDTIVHIIPTNFFEALNKGELL
                     PIIFFAVFFGLGLAAVGKKAEPVKEFLSGSLEAVFWMINKILKLAPLGVFAFICTTII
                     TFGASALLPLLKLVLVVVFAMVFFVFAILGLVAWMCGINIMNIIRILKSELLLAFSTS
                     SSEAVLPVMMKKMENFGSPKEITSFVIPIGYTFNLDGSALYQSIAALFVAQMYGMHLT
                     LSEQIVLMLTLMITSKGMAAVPGTSIVVLLTTLGAMGLPAQGLALIIGVDRILDMVRT
                     CVNVIGNALSTIVIAKWENVYDKAKGQEYLKSI"
BASE COUNT          721 a          369 c          339 g          652 t
ORIGIN      
        1 aggtataaca gaattaacta catttaagga gattgatgaa ccatgaaaaa gaaaaaaggt
       61 tttggtcttg gtattagttt aatcgccatc atgttaattg tatgtattgt attagtaatc
      121 atgatgatga ctggcggaaa gaaagataca tactatggaa ttatgaaaga taatactact
      181 attgaaaaaa tgattagtga aaaagatgaa agtattgaaa aaaatgttaa attaccttca
      241 gattcagatg ttaaagttaa aaaaggtgat tttgtaattg tttataaatt agcagattca
      301 gataaaattg ttaaagttaa aaaagttgac catgacgatg taccacatgg tttaatgatg
      361 aaaattcatg acatgggcaa aatgcacatg aaacactaat tgtaatttaa attacaaatt
      421 ttagttgcca tcaaggtata tacgagtaaa agcagcggta agttgatttc caatttggaa
      481 tcattttact gctgcttttt atatttgaaa tactttcata ttgaatagct ccacttgccg
      541 ttcgcctgcg ctttgcgcat gcataaaagc ccctaacaac ctgaggtcac tgcgctccgg
      601 ttcgcctgcg ctttagcgca tgcataaaag cccctaacaa cctgaggtca ctacgcttcg
      661 gttcgcctgc gctttagcgc atgcataaaa gtccctaaca acctgaggtc actacgcttc
      721 cgttcgcctg cgctttagcg catgcataaa agcccctaac aaccaaaggt tgtaaggggc
      781 tttaaatgtc agatagtatt ttttaaattg attttaaata ttcttgacct tttgctttgt
      841 catatacgtt ttcccattta gctataacga ttgttgataa tgcattacca ataacgttta
      901 cacatgtacg taccatatct aagatacggt caacaccaat aattaatgct aaaccttgtg
      961 ccggtaagcc catggcacct aatgttgtta ataaaacaac gattgaagtt cctggtactg
     1021 ccgccatacc tttagacgta atcattaatg ttaacatcaa cacaatttgc tctgataatg
     1081 ttaagtgcat tccatacatc tgtgcaacga ataatgctgc aatagattga taaagtgctg
     1141 atccatctaa gttaaacgta taaccaattg gtataacaaa agaagtaatt tcttttggag
     1201 aaccgaagtt ttccattttc ttcatcatta caggaagtac agcttccgaa cttgatgtag
     1261 aaaatgctaa aagcaattca cttttcaaga ttctaataat attcatgata ttaataccac
     1321 acatccatgc aactagtcct agtatagcga atacaaagaa caccattgca aagacaacaa
     1381 ctaatactaa ttttaatagt ggtaataatg cggatgcacc aaatgtaata attgtagtac
     1441 aaatgaatgc aaacactcca agtggtgcta attttaaaat tttattaatc atccagaaca
     1501 cagcttcaag cgatccgctt aaaaattctt taactggttc tgctttttta cctacagcag
     1561 ctaatcctaa tccaaagaat actgcgaaga agataatagg taataattca cccttattta
     1621 aagcttcaaa aaagtttgtc ggaataatat gtacaatggt atcaataaaa tgatttccat
     1681 atgtagattg ttctgctgca tgtgcagttg attgatattt agaaatatca cctttaggta
     1741 attttgttgg gtctaatcca gcacctggtt taaataggtt accgaagata atccctaaac
     1801 ctattgcgat tgttgtaata atttcaaagt ataaaattgt tttccagcca taacgcccta
     1861 cagttttcga ttccccaacg ttcgaaatag aaagcgctag tgagcaaaat acaactggta
     1921 taacgatcat tttaattaaa tttaaaaata cgtcaccaaa tggtttaatg taatttgcta
     1981 cgttttcttg tccatataat aaaagtccta cgacaacacc taacactaat gcaataacaa
     2041 cttgcatagg taggctaatt tttctcttga atagagccat a
//
LOCUS       DAXWFK010000250         2064 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.252, whole genome shotgun sequence.
ACCESSION   DAXWFK010000250 DAXWFK010000000
VERSION     DAXWFK010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.252"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(6..1217)
                     /locus_tag="ACGEXS_002603"
     CDS             complement(6..1217)
                     /locus_tag="ACGEXS_002603"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFF8013555.1"
                     /translation="MSNTQKKNVPELRFPGFEGEWEEKQLGDLTDRVIRKNKNLESKK
                     PLTISGQLGLIDQTEYFSKSVSSKNLENYTLIKNGEFAYNKSYSNGYPLGAIKRLTRY
                     DSGVLSSLYICFSIKSEMSKDFMEAYFDSTHWYREVSGIAVEGARNHGLLNVSVNDFF
                     TILIKYPSLEEQQKIGKFFSKLDRQIELEEQKLELLQQQKKGYMQKIFSQELRFKDEN
                     GEDYPDWENSKIEKYLKERNERSDKGQMLSVTINSGIIKFSELDRKDNSSKDKSNYKV
                     VRKNDIAYNSMRMWQGASGKSNYNGIVSPAYTVLYPTQNTSSLFIGYKFKTHRMIHKF
                     KINSQGLTSDTWNLKYKQLKNINIDIPVLEEQEKIGDFFKKMDILISKQKMKIEILEK
                     EKQSFLQKMFL"
     gene            complement(1210..>2064)
                     /locus_tag="ACGEXS_002604"
     CDS             complement(1210..>2064)
                     /locus_tag="ACGEXS_002604"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFF8013556.1"
                     /translation="GSLLLRVGKETQVYRYFGQERNNTTYNLARMNMLLHDVRYENFD
                     IRNDDTLENPAFLGNTFDAVIANPPYSAKWTADSKFENDERFSGYGKLAPKSKADFAF
                     IQHMVHYLDDEGTMAVVLPHGVLFRGAAEGVIRRYLIEEKNYLEAVIGLPANIFYGTS
                     IPTCILVFKKCRQQDDNVLFIDASNDFEKGKNQNHLSDAQVERIIDTYKRKETIDKYS
                     YSATLQEIADNDYNLNIPRYVDTFEEEAPIDLDQVQQDLKNIDKEIAEIEQEINAYLK
                     ELGVLKDE"
BASE COUNT          580 a          391 c          284 g          809 t
ORIGIN      
        1 caaagttata agaacatttt ttgtaaaaag gattgtttct ctttttctaa tatttcaatt
       61 ttcattttct gtttacttat caatatatcc atttttttaa agaaatcacc tatcttttct
      121 tgttcctcca atacaggtat atctatattt atatttttta attgtttata ttttaagttc
      181 catgtatctg atgttaatcc ttgtgaatta attttaaatt tatgaatcat tctatgtgtt
      241 ttaaacttat atccaataaa taatgagcta gtattttgtg ttggataaag cacagtatat
      301 gcagggctaa caatcccatt ataatttgat ttaccactag ccccttgcca cattctcata
      361 gaattatatg caatatcatt tttcctaact actttataat tacttttatc tttacttgaa
      421 ttatcttttc tatccaattc actaaatttt ataatgccac tatttatagt tactgaaagc
      481 atttgccctt tgtcagaacg ttcgtttctc tcttttaaat atttttctat tttgctattt
      541 tcccaatctg gataatcttc accattctca tctttgaatc gcagttcctg tgagaaaatt
      601 ttctgcatat agcctttttt ctgttgttga agtaattcaa gcttttgttc ttctaattca
      661 atttgtcggt cgagtttgct gaagaacttg cctatttttt gctgttcttc taaacttgga
      721 tatttaatta gaatagtaaa aaaatcattc acagaaacat ttaataatcc gtgatttctt
      781 gcaccctcaa ctgcaattcc agaaacttct ctataccagt gtgtcgaatc aaaatatgct
      841 tccatgaagt ctttagacat ttcactttta atagaaaaac aaatatacaa agaggacaat
      901 acaccactat catatctagt taatctttta atagccccta atgggtatcc attagaataa
      961 cttttgttat acgcgaattc tccattcttt attagtgtat aattttctag atttttcgac
     1021 gaaactgatt tactaaaata ttctgtttga tcaattaaac ctaactgtcc ggatattgtt
     1081 aaaggctttt tcgattctaa gtttttattt ttcctaatta ctctatctgt aagatcccct
     1141 aactgcttct cttcccattc gccttcaaac cctgggaacc tcaattctgg cacatttttc
     1201 ttttgtgtat tactcatctt tcaacacccc aagttctttc aggtatgcat tgatttcttg
     1261 ctcaatttct gcgatttctt tatcgatatt tttcaaatct tgttggactt gatctaaatc
     1321 aatcggtgct tcttcttcga atgtatcgac atatctcggt atatttaggt tgtaatcgtt
     1381 atcggcaatc tcttgtagtg tcgcgctgta gctatattta tcaattgttt ccttacgctt
     1441 atatgtgtct ataatacgtt cgacttgggc atcgcttaaa tgattttgat tttttccttt
     1501 ttcaaaatca ttggatgcat cgataaatag tacgttgtcg tcttgttggc gacatttttt
     1561 aaatactaaa atacatgttg gaatacttgt cccatagaaa atattcgctg gcaaaccaat
     1621 cacagcttct aagtagttct tttcttcaat taaataacga cgaatgacac cttctgcagc
     1681 acctcggaat aatacaccat gtgggagtac aacggccatg gtaccttcat cgtctaggta
     1741 atgtaccatg tgttgaataa aggcaaagtc tgctttagac ttaggcgcaa gtttgccgta
     1801 accactgaat cgttcgtcat tttcaaactt tgaatctgca gtccatttcg cactatacgg
     1861 tgggttcgca ataaccgcat caaatgtatt gcctaaaaag gctgggtttt ccaatgtgtc
     1921 atcattacgg atatcgaagt tctcataacg cacatcatgt aataacatat tcatgcgtgc
     1981 taagttgtat gtagtattgt tacgttcttg accgaaataa cgatacactt gtgtttcttt
     2041 accaacacgt aacaacagtg aacc
//
LOCUS       DAXWFK010000251         2056 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.253, whole genome shotgun sequence.
ACCESSION   DAXWFK010000251 DAXWFK010000000
VERSION     DAXWFK010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.253"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(253..>2056)
                     /gene="pepF"
                     /locus_tag="ACGEXS_002605"
     CDS             complement(253..>2056)
                     /gene="pepF"
                     /locus_tag="ACGEXS_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFF8013557.1"
                     /translation="QQLSREEQERKYPEYTWDLTTIFKDDEAFEAAFKEVENELGKEE
                     QFKGHIGDSAETLYNALELEDTLGTKLEKVYVYAHLKQDQDTTNDKYTGMESRAHQLI
                     IKFSSAWSFLVPEILQIDEDKIQSFVNSYDKLQKFAFDLKLINEKRPHILDAETEKLL
                     TEAQDALSTPSNVYGMFSNADLVFEDAIDKDGNAHPLTQGTFIKYLETDDRKLRESAF
                     RNVYKAYGAHNNTLGATLAGEVKKNVFNARTHNYKTAREKALSNNHIPENVYDNLVKT
                     VHKYLPLLHRYTELRKELLGLDDLKMYDLYTPLIKDIKFEMPYEEAKEWMLKALEPMG
                     EEYLNVVKEGLNNRWVDVYENKGKRSGGYSSGAHLTNPFILLNWSNTISDLYTLVHEF
                     GHSAHSYFSRKFQPSNSSDYTIFVAEVASTCNEALLSDYMDKHLDDEKRLLLLNQELE
                     RFRATLFRQTMFAEFEHKIHAIEEAGEPLTPTRMNEEYAKLNKLYFGDSVETDEDISK
                     EWSRIPHFYMNYYVYQYATGYSAAQSLSHQILTEGKPAVDRYINEFLKKGSSNYPIEI
                     LKNAGVDMTTPEPIEQACEVFEQKLNAFEKLMKA"
BASE COUNT          617 a          367 c          285 g          787 t
ORIGIN      
        1 atatttaata catttttata caacttcact tccctcttat taaaaaagaa cataaaaaaa
       61 cgtcgtgtct gaatacacga cgctaaacaa aggggatggg agaaattttt cacttcaaac
      121 aaaggggtat gtttgttatg tgattaattt catgttcata atataacatg ctatctctac
      181 catttcaacc ttttaatagt acttaattta aattgtcaca atctgttcat aatgtaagcg
      241 aatacaatag tactaagctt tcattaattt ttcaaaagcg ttcaattttt gttcaaaaac
      301 ttcacaagct tgttcaattg gttcaggtgt tgtcatatct acaccagcat tttttaatat
      361 ctcaattgga taatttgagc tacctttttt caagaattca ttaatatatc tatctactgc
      421 tggcttacct tctgttaaaa tttgatgact taagctttga gctgcactgt aaccagttgc
      481 gtattgatat acataataat tcatatagaa gtgtggaata cgtgaccatt ccttactaat
      541 atcttcatca gtttctacag aatcaccgaa gtataattta tttaatttgg catattcttc
      601 attcattcta gttggcgtta atggttcacc tgcttcttca attgcatgaa ttttatgctc
      661 aaattctgcg aacattgttt gtcggaataa tgtagctctg aaacgttcta attcttggtt
      721 taataataat aagcgttttt catcatcaag atgtttatcc atataatcac ttaaaagtgc
      781 ttcgttacaa gttgatgcaa cttcagcgac aaaaatagtg tagtcactag aatttgacgg
      841 ttggaatttt ctactgaagt aactatgtgc tgaatgccca aattcatgaa ctaatgtgta
      901 taagtctgaa atagtattag accagttaag tagaataaat ggattagtta aatgtgcacc
      961 tgatgaatag ccacctgaac gtttaccttt attctcatag acatcgaccc aacgattgtt
     1021 taagccttct ttaactacat ttaaatattc ttcacccatt ggctctaatg cttttaacat
     1081 ccactcttta gcttcttcat aaggcatttc aaacttaata tctttaatta atggtgtata
     1141 taaatcatac attttcaagt catctaaacc tagcaattct ttgcgcaatt cagtatatct
     1201 atgtagcaat ggtaaatatt tatgtacagt ttttactaga ttgtcatata cattttctgg
     1261 aatatgatta ttactcaatg ctttttctct tgcagttttg taattgtgtg tacgagcatt
     1321 aaatacattt ttcttcactt cacctgctag cgtagcgcca agcgtattat tatgagcacc
     1381 atatgcttta tatacatttc taaaagcact ttctcttagt ttgcgatcat ctgtttctaa
     1441 atacttaata aatgtacctt gtgttaacgg gtgtgcattt ccatctttat ctatcgcatc
     1501 ttcaaatact aaatcagcgt tgctaaacat accgtataca tttgatggcg ttgataacgc
     1561 gtcctgcgct tctgttaata acttttcagt ttcagcatct aaaatatgag gacgtttttc
     1621 attaatcaac ttcaaatcaa atgcgaattt ttgtaattta tcatatgaat ttacaaatga
     1681 ttgaatttta tcttcatcaa tttgtaaaat ctctggcact aagaaactcc atgccgagct
     1741 aaatttaata attaattgat gtgctcttga ctccatacca gtatacttgt cgttcgttgt
     1801 atcttggtct tgttttaggt gcgcgtatac atatactttt tctaatttag tacctaatgt
     1861 atcttctaat tctaacgcat tgtataatgt ctcagcacta tcaccaatgt gtcctttaaa
     1921 ttgttcttct ttgcctaact cattttcaac ttctttaaat gcagcctcaa aagcttcatc
     1981 atctttgaaa attgttgtta agtcccatgt atattcagga tatttacgtt cctgttcttc
     2041 tctcgataat tgttga
//
LOCUS       DAXWFK010000252         2034 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.254, whole genome shotgun sequence.
ACCESSION   DAXWFK010000252 DAXWFK010000000
VERSION     DAXWFK010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.254"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..996
                     /gene="argF"
                     /locus_tag="ACGEXS_002606"
     CDS             <1..996
                     /gene="argF"
                     /locus_tag="ACGEXS_002606"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFF8013558.1"
                     /translation="NLRNRSFLTLLDFSRQEVEFLLTLSEDLKRAKYIGTEKPMLKNK
                     NIALLFEKDSTRTRCAFEVAAHDQGANVTYLGPTGSQMGKKETTKDTARVLGGMYDGI
                     EYRGFSQRTVETLAEYSGVPVWNGLTDEDHPTQVLADFLTAKEVLKKDYADINFTYVG
                     DGRNNVANALMQGAAIMGMNFHLVCPKELNPTDELLNRCKNIAAENGGNILITDDIDQ
                     GVKGSDVIYTDVWVSMGEPDEVWKERLELLKPYQVNKEIMDKTGNPNVIFEHCLPSFH
                     NADTKIGQQIFEKYGIREMEVTDEVFESKASVVFQEAENRMHTIKAVMVATLGEF"
     gene            1019..1951
                     /gene="arcC"
                     /locus_tag="ACGEXS_002607"
     CDS             1019..1951
                     /gene="arcC"
                     /locus_tag="ACGEXS_002607"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFF8013559.1"
                     /translation="MAKIVVALGGNALGKSPQEQLELVKNTAKSLVGLITKGHEIVIS
                     HGNGPQVGSINLGLNYAAEHNQGPAFPFAECGAMSQAYIGYQLQESLQNELHSIGMDK
                     QVVTLVTQVEVDENDPAFNNPSKPIGLFYNKEEAEQIQKEKGFIFVEDAGRGYRRVVP
                     SPQPISIIELESIKTLIKNDTLVIAAGGGGIPVIREQHDGFKGIDAVIDKDKTSALLG
                     ANIQCDQLIILTAIDYVYINFNTENQQPLKTTNVDELKRYIDENQFAKGSMLPKIEAA
                     ISFIENNPKGSVLITSLNELDAALEGKVGTVIKK"
BASE COUNT          755 a          284 c          397 g          598 t
ORIGIN      
        1 aatttacgaa acagaagttt tttaacttta ttagactttt cacgacaaga ggtagaattc
       61 ttattaacac tctccgagga tttaaaacgt gctaaatata ttggcactga aaagcctatg
      121 ttaaaaaata aaaatattgc actgttattt gaaaaagatt ctacaagaac gcgatgtgca
      181 tttgaagttg cagcgcatga tcaaggtgca aatgtaactt atttaggccc aactggatca
      241 caaatgggta aaaaagaaac aactaaagat actgcacgtg tgcttggtgg aatgtatgat
      301 ggcattgaat accgtggttt ttcacaaaga acagtagaaa ctttagctga gtattcaggc
      361 gtaccagtgt ggaatggttt aactgatgaa gatcatccta ctcaagttct tgctgatttc
      421 ttaacagcaa aagaagtctt aaaaaaagat tatgcagata ttaactttac atatgttgga
      481 gatggtcgta ataacgttgc aaatgcatta atgcaaggtg ctgccattat gggtatgaac
      541 ttccatttag tttgtccaaa agaattaaat ccaacagatg aattattaaa tcgctgtaaa
      601 aatattgccg ctgaaaatgg tggcaacata ttaatcacag atgatattga ccaaggtgta
      661 aaaggttcgg atgtaattta cactgatgtt tgggtatcaa tgggtgaacc tgatgaagta
      721 tggaaagaac gacttgaatt attgaaacca tatcaagtaa ataaagaaat aatggataaa
      781 actggtaatc caaatgttat ttttgagcat tgcttaccat ctttccataa tgctgatacg
      841 aaaataggtc aacaaatttt tgaaaaatat ggtattcgag aaatggaagt tacagatgaa
      901 gtattcgaaa gtaaagcttc agttgtattc caagaagctg agaacagaat gcatacaatc
      961 aaagcagtca tggttgctac attgggtgaa ttttaaatga tataaggaag tgaatatgat
     1021 ggcgaaaata gtagtagcat taggtggtaa tgctttagga aaatcacctc aagaacaact
     1081 cgagcttgtt aaaaatactg cgaaatcatt agtaggatta ataacaaaag gacatgagat
     1141 tgttattagt catggtaatg gaccacaggt tggaagcatt aatttgggac ttaactatgc
     1201 tgcagaacat aaccaaggtc cggcatttcc atttgctgaa tgtggcgcaa tgagtcaagc
     1261 ttacatcggc tatcaattac aagaaagctt acaaaatgaa ttgcattcta ttggaatgga
     1321 taaacaagtg gtaacactag tgacacaagt tgaagttgat gaaaatgatc cggcatttaa
     1381 caatccttca aaaccaattg ggttatttta caacaaagaa gaagctgaac aaattcaaaa
     1441 agaaaaagga tttatatttg ttgaagatgc tggaagagga tatagacgcg ttgttccttc
     1501 accacaaccc atctctatta ttgaattaga gagtattaaa acacttatta aaaatgatac
     1561 actcgttatt gctgctggtg gtggaggtat accagtaatt agagagcaac atgatggttt
     1621 taaaggtatt gatgcagtta tagacaaaga taaaacaagt gcgttgttgg gtgctaatat
     1681 tcaatgcgat caattgatta ttttaacagc aattgattat gtatatatta attttaacac
     1741 tgaaaaccaa cagcctttga aaacaacaaa tgttgatgaa ttaaaacgat atatagacga
     1801 aaatcaattt gcaaaaggaa gtatgttacc aaaaattgaa gcagccatat catttattga
     1861 aaacaatcca aaaggaagtg tgcttataac atcattaaat gaattagatg ctgccttaga
     1921 gggtaaagta ggtactgtga ttaaaaagta attgaattga aacgcttttc aattactata
     1981 tgtcaaatgc atgattttta ttatttatgt gcaccccctg aaaataatgc ctct
//
LOCUS       DAXWFK010000253         2029 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.255, whole genome shotgun sequence.
ACCESSION   DAXWFK010000253 DAXWFK010000000
VERSION     DAXWFK010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.255"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            22..213
                     /locus_tag="ACGEXS_002608"
     CDS             22..213
                     /locus_tag="ACGEXS_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5325 family protein"
                     /protein_id="HFF8013560.1"
                     /translation="MKQKKSKNIFWVFSILAVVFLVLFSFAVGASNVPMMILTFILLV
                     ATFGIGFTTKKKYRENDWL"
     gene            complement(367..1194)
                     /locus_tag="ACGEXS_002609"
     CDS             complement(367..1194)
                     /locus_tag="ACGEXS_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HFF8013561.1"
                     /translation="MALYGFAQGLIQEAGIRIKQLMEQNLTIETKSNPNDLVTNVDKA
                     TEDFIFDTILETYPNHQVLGEEGHGHDIDTSKGTVWIVDPIDGTLNFVHQQENFAISI
                     GIYIDGKPYAGFVYDVMADVLYHAKVGEGAYRGSQPLKPLNDSNLRQSIIGINPNWLT
                     KPILGEIFKEIVNDSRSARAYGSAALEIVSVATGNLEAYMTPRLQPWDFAGGLVILYE
                     VNGQASNLLGEPLTISGPNSILVGNRGLHQEISNDYLEPHHDALIQLHEQRFKRKSK"
     gene            1351..1965
                     /locus_tag="ACGEXS_002610"
     CDS             1351..1965
                     /locus_tag="ACGEXS_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YktB family protein"
                     /protein_id="HFF8013562.1"
                     /translation="MTKYTFKPKDFKAFNVEGLDARMEALNEYIRPQLRELGEYFSDF
                     FTSQTGETFYPHVAKHARRSVNPPKDTWVAFATNKRGYKMLPHFQIGMFEDQLFVMFG
                     IMHEAKDKATRAKVFERKFKAIQQLPDDYRVCLDHMKPDKPFIKDLTDDDLIEAIQRA
                     INVKKGEFFIARAITPQDKRLKSDKAFIAFLEETFDQFLPFYSA"
BASE COUNT          687 a          340 c          315 g          687 t
ORIGIN      
        1 taagcaaaag gtggggatag gatgaaacaa aaaaaatcta aaaatatctt ttgggtattt
       61 tctatattag ctgttgtttt cttagtatta tttagttttg ctgttggtgc atcaaatgta
      121 ccaatgatga ttttaacatt tatattactc gttgcaacct ttggaattgg atttactaca
      181 aagaaaaaat atcgagaaaa cgattggcta taagttatat cgacaataac acgcgttgat
      241 ttaagaccac acatgttcat gatgaacatt taactggttt tatattggcg cgtgttttta
      301 ttatttaagg tataaaagcg taatatgtta cctaattgtt aggactacat attacgcttt
      361 tggtaattat tttgattttc ttttaaatcg ttgttcatgt aattgtatta acgcatcatg
      421 gtgaggctct aaataatcat tgctaatttc ttgatggaga ccacgatttc caactaagat
      481 tgaatttgga ccactaattg ttaatggttc tcctagtaaa ttggaagctt gtccatttac
      541 ttcatataaa ataaccaatc cgccagcaaa atcccatggt tgaagtcttg gcgtcatata
      601 tgcttctaaa ttacctgtag caactgaaac gatttcaagc gctgcactac catatgccct
      661 tgcacttcta gaatcattaa caatttcttt aaagatttct cctaaaattg gtttagttaa
      721 ccagttcgga ttgatcccaa taatgctttg tcttagatta gaatcattca atggtttcaa
      781 gggttggcta ccacgatatg caccttcccc tactttagca tgatataaga catcagccat
      841 aacatcatat acaaaacctg cataaggttt accatcgata taaataccaa ttgaaattgc
      901 gaaattttct tgttgatgaa caaaattcaa tgtaccgtct attgggtcaa caatccatac
      961 cgtacctttg gaagtatcga tgtcatgacc atgcccttct tcacctaata cttgatgatt
     1021 gggatatgtt tctaaaattg tatcaaaaat gaaatcttct gttgctttat ctacatttgt
     1081 aacaaggtca ttcggatttg actttgtttc aattgttaaa ttttgctcca tcaattgttt
     1141 aattctaatt cctgcttctt gaataagtcc ttgggcaaat ccataaagtg ccataacatc
     1201 atctcctaac atctttatta tacatcaaca ttttataaaa ataacttctt atgataaaaa
     1261 tgaaaatact aattttaatg agagcgtctt aagccgcaat tgataaaaca tatgctacaa
     1321 tatttttaaa cactatacag gaggtactct atgacaaaat atacatttaa acctaaagat
     1381 ttcaaagcgt tcaacgtaga aggcttagac gcacgaatgg aagctttaaa cgaatacata
     1441 cgaccacaac tccgtgaatt aggagaatat tttagtgact tctttacaag tcaaactggt
     1501 gaaacatttt atcctcacgt agcaaagcat gctagaagaa gtgtgaatcc tcctaaagat
     1561 acatgggttg cttttgcaac aaacaaaaga ggctataaaa tgttacctca tttccaaatt
     1621 ggtatgtttg aagatcaact gtttgttatg tttggaatca tgcatgaagc aaaagataaa
     1681 gcaacacgtg caaaagtttt tgaaagaaaa tttaaagcta ttcaacaatt acctgatgat
     1741 tatcgtgttt gcttagatca tatgaaacct gataaaccat ttattaaaga tttaacggat
     1801 gatgatttaa tagaagcgat acaaagagcc atcaatgtga aaaaaggtga attctttata
     1861 gcgcgtgcaa tcacaccaca agataaaaga ttaaaaagtg acaaagcatt tattgcattt
     1921 ttagaagaaa ccttcgatca gttcttacca ttttattctg cataaataac tttgtttaaa
     1981 taatagagca cttaatcaca tccatgattt cgtgctcttt tttcttaat
//
LOCUS       DAXWFK010000254         2022 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.256, whole genome shotgun sequence.
ACCESSION   DAXWFK010000254 DAXWFK010000000
VERSION     DAXWFK010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.256"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            103..1962
                     /locus_tag="ACGEXS_002611"
     CDS             103..1962
                     /locus_tag="ACGEXS_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HFF8013563.1"
                     /translation="MPKNKILIYLLSTTLVLPTLVSPTAYADTPQKDTTAKTTSHDSK
                     KSNDDETSKDTTSKDIDKADKNNTSNQDNNDKKFKTIDDSTSDSNNIIDFIYKNLPQT
                     NINQLLTKNKYDDNYSLTTLIQNLFNLNSDISDYEQPRNGEKSTNDSNKNSDNSIKND
                     TDTQSSKQDKADNQKAPKSNNTKPSTSNKQPNSPKPTQPNQSNSQPASDDKANQKSSS
                     KDNQSMSDSALDSILDQYSEDAKKTQKDYASQSKKDKNEKSNTKNPQLPTQDELKHKS
                     KPAQSFNNDVNQKDTRATSLFETDPSISNNDDSGQFNVVDSKDTRQFVKSIAKDAHRI
                     GQDNDIYASVMIAQAILESDSGRSALAKSPNHNLFGIKGAFEGNSVPFNTLEADGNQL
                     YSINAGFRKYPSTKESLKDYSDLIKNGIDGNRTIYKPTWKSEADSYKDATSHLSKTYA
                     TDPNYAKKLNSIIKHYQLTQFDDERMPDLDKYERSIKDYDDSSDEFKPFREVSDSMPY
                     PHGQCTWYVYNRMKQFGTSISGDLGDAHNWNNRAQYRDYQVSHTPKRHAAVVFEAGQF
                     GADQHYGHVAFVEKVNSDGSIVISESNVKGLGIISHRTINAAAAEELSYITGK"
BASE COUNT          811 a          364 c          284 g          563 t
ORIGIN      
        1 cgattgatac ctatcaacta ttttcagcat acgataaatt ataacaaatc atagtttatt
       61 atcacactta attattatat ttttcaaggg agaatacgaa atatgcctaa aaataaaatt
      121 ttaatttatt tgctatcaac tacgctcgta ttacctactt tagtttcacc taccgcttat
      181 gctgatacac ctcaaaaaga tactacagct aagacaacat ctcatgattc aaaaaaatct
      241 aatgacgatg aaacttctaa ggatactaca agtaaagata ttgataaagc agacaaaaat
      301 aatacaagta accaagacaa taacgacaaa aaatttaaaa ctatagacga cagcacttca
      361 gactctaaca atatcattga ttttatttat aagaatttac cacaaaccaa tataaaccaa
      421 ttgctaacca aaaataaata cgatgataat tactcattaa caactttaat ccaaaactta
      481 ttcaatttaa attcggatat ttctgattac gaacaacctc gtaatggcga aaagtcaaca
      541 aatgattcga ataaaaacag tgacaatagc atcaaaaatg acactgatac gcaatcatct
      601 aaacaagata aagcagacaa tcaaaaagca cctaaatcaa acaatacaaa accaagtaca
      661 tctaataagc aaccaaattc gccaaagcca acacaaccta atcaatcaaa tagtcaacca
      721 gcaagtgacg ataaagcaaa tcaaaaatct tcatcgaaag ataatcaatc aatgtcagat
      781 tcggctttag actctatttt ggatcaatac agtgaagatg caaagaaaac acaaaaagat
      841 tatgcatctc aatctaaaaa agacaaaaat gaaaaatcta atacaaagaa tccacagtta
      901 ccaacacaag atgaattgaa acataaatct aaacctgctc aatcattcaa taacgatgtt
      961 aatcaaaagg atacacgtgc aacatcatta ttcgaaacag atcctagtat atctaacaat
     1021 gatgatagcg gacaatttaa cgttgttgac tcaaaagata cacgtcaatt tgtcaaatca
     1081 attgctaaag atgcacatcg cattggtcaa gataacgata tttatgcgtc tgtcatgatt
     1141 gcccaagcaa tcttagaatc tgactcaggt cgtagtgctt tagctaagtc accaaaccat
     1201 aatttattcg gtatcaaagg tgcttttgaa gggaattctg ttccttttaa cacattagaa
     1261 gctgatggta atcaattgta tagtattaat gctggattcc gaaaatatcc aagcacgaaa
     1321 gaatcactaa aagattactc tgaccttatt aaaaatggta ttgatggcaa tcgaacaatt
     1381 tataaaccaa catggaaatc ggaagccgat tcttataaag atgcaacatc acacttatct
     1441 aaaacatatg ctacagatcc aaactatgct aagaaattaa acagtattat taaacactat
     1501 caattaactc agtttgacga tgaacgcatg ccagatttag ataaatatga acgttctatc
     1561 aaggattatg atgattcatc agatgaattc aaacctttcc gtgaggtatc tgatagtatg
     1621 ccatatccac atggccaatg tacttggtac gtatataacc gtatgaaaca atttggtaca
     1681 tctatctcag gtgatttagg tgatgcacat aattggaata atcgagctca ataccgtgat
     1741 tatcaagtaa gtcatacacc aaaacgtcat gctgctgttg tatttgaggc tggacaattt
     1801 ggtgcagatc aacattacgg tcatgtagca tttgttgaaa aagttaacag tgatggttct
     1861 atcgttattt cagaatccaa tgttaaagga ttaggtatca tttctcatag aactatcaac
     1921 gcagctgccg ctgaagaatt atcatatatt acaggtaaat aagtattata ttaaacccgt
     1981 aaaatttata agtataaaca aggagttcgg acttaaacat at
//
LOCUS       DAXWFK010000255         2010 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.257, whole genome shotgun sequence.
ACCESSION   DAXWFK010000255 DAXWFK010000000
VERSION     DAXWFK010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.257"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(215..1588)
                     /locus_tag="ACGEXS_002612"
     CDS             complement(215..1588)
                     /locus_tag="ACGEXS_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HFF8013564.1"
                     /translation="MTAETNYFWLNCGYNRWNHNEPLVGQTALFESGAHFNPSQGFRA
                     FKKAKVGDQVIFYQVQTDTGLLGCGEIISVETGAQNKIRVQFRFNEQLKPLTADYLKR
                     SEALEFRISNMKETLFNQITAEEFDLISGLGKGEIKIPRYFFLAETEEFEPGNQYTIY
                     THTYNGIKRNGYHFYTQLEEGDNIIFYNRTKNQSVVGIGEVSKHIHEKPPIPGRTNST
                     VIEVSYEKDITPITLSTLNKHPKLKNLYFLQENAKQAIASMSQAQYDAIIEMSDNNGL
                     KSPFEMVQKPDMLESEKEEALKPFILLVVDRKEEGLKAANDLLQKANANPVITTGHPD
                     FSEDMLYGKYLPNETGALYYREGFITQLMPKKDKSYLVIDNFNRIDTDIFQTYINVLE
                     GYEVTLPRYNKDGNMIKWSRQKDSFYYFNPNWHIVGITYDSLEEIKEKYSEQFLKYTR
                     IVKVKHD"
BASE COUNT          621 a          368 c          253 g          768 t
ORIGIN      
        1 ctcatataaa gttttaattc tgtaacataa attcttttat attcatatac tgtctttatt
       61 atttattaaa ataaaaacga gtaatctatc ataagctctt aattagctct tatggttgat
      121 tacccgtcat tttttcttta tgcggttatt caaatatgca attatgcaat ttttaatcct
      181 atccttactt cttttcaaaa actaccatat atcattagtc atgtttgact ttgactatac
      241 gtgtgtattt taaaaactgc tctgaatatt tctccttaat ttcctctaaa ctgtcatatg
      301 taatacctac aatatgccaa ttaggattga aataatagaa agaatctttt tgtcgtgacc
      361 atttaatcat attaccgtct ttattataac gtggtaatgt tacttcatag ccttctaaca
      421 cattaatata cgtttggaaa atgtctgtat ctatacgatt aaaattatca ataactaaat
      481 aacttttatc ctttttaggc attaattgag taataaaccc ttcacgatag tataatgccc
      541 ctgtttcatt tggtaaatac ttgccataaa gcatatcttc tgaaaaatca ggatgacctg
      601 ttgttataac aggatttgca ttcgcttttt gcaataagtc attagccgct tttaagcctt
      661 cttcttttcg atctacaaca agtaaaataa atggctttaa cgcttcttct ttttcacttt
      721 caagcatatc tggtttttga accatttcaa atggagattt caatccatta ttatcgctca
      781 tttcaataat tgcatcatac tgtgcttgtg acatacttgc aatagcctgt tttgcatttt
      841 cttgaaggaa atataagttt ttcaatttag gatgcttatt taatgtactt aatgtaatcg
      901 gtgtaatgtc tttctcataa gacacttcaa tcactgtact atttgttcta ccaggaattg
      961 gtggtttttc atgaatatgc tttgatactt ctccaattcc aacgacagat tgatttttcg
     1021 ttcgattata aaaaataata ttgtcgcctt cttctaactg agtataaaaa tgataaccat
     1081 tacgtttaat accgttgtac gtgtgcgtat aaatcgtata ttggtttcca ggttcaaatt
     1141 cttcagtttc agctaaaaag aaataacgcg gtatcttaat ttcgccttta ccaagaccac
     1201 ttattaaatc aaactcttct gcagtgattt gattgaacaa tgtctctttc atattactta
     1261 tacgaaattc caaagcttca ctacgcttta aataatctgc tgttaatggt ttcaattgtt
     1321 cattaaaacg aaactgtaca cgtattttat tttgtgcacc tgtttcaaca ctaataattt
     1381 caccacatcc aagtagtcca gtatccgtct gaacttgata aaagatgact tgatctccta
     1441 ctttagcctt tttaaacgct ctaaatcctt gagatgggtt aaaatgtgcg cctgattcaa
     1501 ataaagctgt ttgtcctact aacggttcat tatgattcca acggttatat ccacaattca
     1561 accaaaaata attcgtttct gctgtcatct taatactcct taacctgaat aaattttaga
     1621 aacactatga attacattct tttagtgttt cttatgcagt tggacgcgta tgcgaacaac
     1681 tgtataccct ttgttcactg cgattttaat cgcatttcct ataacattgt agcgcccagg
     1741 acattaattt acgtcccaga cccttatcgt tttcacttct aagtaagtcg aactattttg
     1801 ctttacaaca agtgcgactc taaatacagt tggacacaca tacgagcaac tgtatacctt
     1861 ttaatcagtt ttctatattt tatttattat atctgtctta atgataaaaa ttgttacaaa
     1921 cagtttaaca tatttagcta cctattttgc atataaaaaa catgcttgca tacactatgc
     1981 aataaaaatt acaaatttat atatgatacc
//
LOCUS       DAXWFK010000256         1923 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.260, whole genome shotgun sequence.
ACCESSION   DAXWFK010000256 DAXWFK010000000
VERSION     DAXWFK010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.260"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            36..704
                     /gene="queC"
                     /locus_tag="ACGEXS_002613"
     CDS             36..704
                     /gene="queC"
                     /locus_tag="ACGEXS_002613"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFF8013565.1"
                     /translation="MESVLNNEKAIVVFSGGQDSTTCLFYAKKHFKEVELVTFNYGQR
                     HDTEIEVAKQIAQDQGMKHHVLDMSLLSQLTPNALTQHDMEITNNEDGIPNTFVPARN
                     LLFLSFAGALAYQIGAKHIITGVCETDFSGYPDCRDSFIKSMNVTLSLAMDKDFVIHT
                     PLMWLNKAETWKLSDELEVLDYIRTKTLTCYNGIIGDGCGECPACHLRQRGLNQYLES
                     KGAL"
     gene            706..1125
                     /gene="queD"
                     /locus_tag="ACGEXS_002614"
     CDS             706..1125
                     /gene="queD"
                     /locus_tag="ACGEXS_002614"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFF8013566.1"
                     /translation="MLQQIYPSTTHPYQFELNKDFNFSAAHHIPCEEAGICQNVHGHT
                     YFVNLTIVGDKLDDTGFLVNFSHLKKMIHGKFDHQLLNNLPAFKNKIPSTEIVAETIY
                     QIVKENLASLEHQPKCIQVFVRETPTSYVVFRPKEQV"
     gene            1129..1842
                     /gene="queE"
                     /locus_tag="ACGEXS_002615"
     CDS             1129..1842
                     /gene="queE"
                     /locus_tag="ACGEXS_002615"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFF8013567.1"
                     /translation="MAKIPVLEIFGPTIQGEGRVIGRKTMFVRTAGCDYRCSWCDSAF
                     TWDGSAKGDIKLMTAEEIYDELKRIGGDLFNHVTISGGNPALIKGIQELVDLFQDKGI
                     FSALETQGSKFQPWMTQIDDLTISPKPPSSTMTPDLKKLDEVITQCVPSSLNLKVVVF
                     DDKDYDFAKMIHHRYPDIPFYLQVGNPYLSDSVDNHTEKLLERYEQLVDLVMQSNDMN
                     HVYVLPQLHTLLWSNKKGV"
BASE COUNT          686 a          309 c          341 g          587 t
ORIGIN      
        1 ttattaatat tattaatgat aggagcaatt acaaaatgga aagtgtatta aataatgaaa
       61 aagccatcgt tgtctttagt ggtggtcaag acagtacaac atgtctcttt tatgcaaaaa
      121 aacatttcaa agaagttgaa ctcgtaacgt ttaattatgg ccaaagacat gatactgaaa
      181 ttgaagtcgc aaaacaaatt gcacaagatc aaggaatgaa acatcacgtt ttagatatgt
      241 cattattatc acaacttact ccaaacgcat taacacaaca tgatatggaa attactaata
      301 atgaagatgg tatacctaat acatttgttc cagctagaaa tttacttttc ttgtcgtttg
      361 caggcgctct agcttatcaa attggggcta agcatattat tacaggcgta tgtgaaacag
      421 acttttcagg ctacccagac tgtcgcgata gttttataaa atcaatgaac gtaacattaa
      481 gcctagctat ggacaaagat tttgtcattc atactccttt aatgtggtta aacaaagcag
      541 aaacgtggaa attaagtgat gaactcgaag ttttagatta tattcgtaca aaaacattaa
      601 catgctataa cggtatcatt ggggatggct gtggtgaatg tccagcttgt catttacgtc
      661 aacgtggact aaatcaatat cttgaaagta aaggagcgct ttaaaatgtt acaacaaatc
      721 tatcctagta caacgcatcc atatcaattc gaattaaata aagattttaa tttttcggct
      781 gcacatcaca ttccatgtga agaagcaggt atttgtcaaa atgtccatgg tcatacttac
      841 tttgttaatt taacaattgt cggtgataaa ctagatgaca ctggcttctt agtgaacttt
      901 agccatttga aaaagatgat acacggtaaa tttgaccatc aactgttaaa taacttacct
      961 gcttttaaaa acaaaatccc ttcaactgaa atcgtagcgg aaacaattta tcaaattgtt
     1021 aaagaaaatt tggcatcgct cgaacaccaa ccaaaatgta ttcaagtatt tgtaagagaa
     1081 acaccaacaa gttatgtcgt atttagacca aaggaacagg tgtaactaat ggctaaaatt
     1141 cctgtactcg aaatattcgg tccaaccatt cagggtgaag gtcgagtaat cggtagaaaa
     1201 acgatgtttg tgagaacagc tggatgtgat tatcgctgta gctggtgtga ttctgcattt
     1261 acttgggatg gtagtgctaa aggcgatata aaactcatga ccgctgaaga aatttatgat
     1321 gaattaaaac gaattggtgg cgatttattt aaccacgtta caatttctgg tggtaaccca
     1381 gcattaatta aaggtatcca agagttagtt gacttatttc aagataaagg tattttcagc
     1441 gcactggaga cacaaggcag taaattccaa ccttggatga cacaaattga tgatttaacg
     1501 attagtccaa aaccaccaag ttcaactatg acacctgatt taaaaaagtt agatgaagtg
     1561 attacacaat gcgtaccttc atcattaaac ttaaaagtag ttgtattcga cgataaagat
     1621 tatgattttg ccaaaatgat acaccaccgt tatccagata ttcccttcta tttacaagtt
     1681 ggtaacccat atttatcaga cagcgtagat aatcataccg aaaagttgtt agaacgctac
     1741 gagcagttgg tggatttagt tatgcaaagt aatgatatga atcacgttta tgtgttacca
     1801 caacttcata cattactttg gagcaataaa aaaggtgtat aaaggataac ttattttact
     1861 ttttgcaagt catatctaat gaataacaat atattaattg atataatagt tgattgaagt
     1921 ata
//
LOCUS       DAXWFK010000257         1915 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.261, whole genome shotgun sequence.
ACCESSION   DAXWFK010000257 DAXWFK010000000
VERSION     DAXWFK010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.261"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..1777
                     /locus_tag="ACGEXS_002616"
     CDS             <1..1777
                     /locus_tag="ACGEXS_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8013568.1"
                     /translation="DWSLSNLFNTGAVKLNKIAKFFNEVYPNLNSLLDCDINTLEKHW
                     FNWLTENNIPIKRRSSTIVFGDYEYKSGLASFLKNMYINLIKFIDKREEWEKDKWDIR
                     NLEKYGLSYNKTLTGNYLNFEKIESIKMRELAKKYLKNRLITGDIGFATARFYIRVLT
                     RFFQNISKNKETRNSLNELDRCHIEAYIEFLFEYAANKNLQSTKNFVREELKTIRRFL
                     NDIITQNYAIAPYQDIRFLIYPQDLPKHEKKNSSQIDYIPDFVLEQLFEHINDLHKDL
                     IPVVWIAFKTGLRISDVLTLQNNCLAKVNGKYSIITDIAKTFVKGHRIPIDNKLADII
                     AVLIADSKSKSTKDNNPNNYIFAIYKGKRKGMPFTQHMVRAHLNHLSKTKNIIDEQGE
                     IFHFKTHQFRHTYAVKLLNGGADILTIQELLAHSSPEMTLRYAKLLDDTKRKAFESVI
                     DQGAFSFDVDGKIKNIQHSSELSEKALNSLWQEHKLNAMDNPYGTCHARLSGDCPYME
                     APPCLTCNSGKPCKDLAIGFSDLDVEKYELHIKSTVKSIELAKNNNRQDMVEKHINIL
                     NKYEEILGNIKDGNIIFGRSNRIKV"
     gene            1786..>1915
                     /locus_tag="ACGEXS_002617"
     CDS             1786..>1915
                     /locus_tag="ACGEXS_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6262 family protein"
                     /protein_id="HFF8013569.1"
                     /translation="MEKFNRQEQLKQLHAERKVKTEKKVDKAINDLVQKNKAINFNI"
BASE COUNT          799 a          220 c          309 g          587 t
ORIGIN      
        1 ggattggtcc ttatctaatt tatttaatac tggagcagta aagcttaata agattgctaa
       61 gttctttaat gaagtttatc caaatttaaa ttctttactt gattgtgata taaatacatt
      121 agaaaagcac tggtttaatt ggctgactga aaataatata ccaataaaaa gaagatcatc
      181 aacaatagtt tttggtgatt atgaatacaa aagcgggctg gcttcttttt taaagaatat
      241 gtacattaat ctaattaagt tcattgataa gcgagaagaa tgggaaaaag ataaatggga
      301 tattagaaat ttagaaaaat atggattgag ttataataaa acactaactg gcaattattt
      361 aaactttgaa aaaattgagt caattaaaat gcgagaatta gcaaaaaaat atttaaaaaa
      421 tcgtttgata actggtgata taggatttgc tacagcaagg ttttatataa gagttttaac
      481 taggtttttt caaaatatat ctaaaaataa agaaacaagg aatagtttaa atgaactaga
      541 tagatgtcat atcgaagcat atatagaatt tttgtttgaa tatgcagcta ataaaaattt
      601 gcaaagtact aagaactttg tgagagaaga attaaaaaca attagaaggt tcttaaacga
      661 tattataact caaaactatg ctatagcacc gtaccaagat atacgatttt taatttatcc
      721 tcaagactta cctaaacacg aaaagaaaaa tagtagccaa attgattata tcccagattt
      781 tgtattggaa cagctttttg agcatataaa tgatttgcat aaagatttaa tacctgtagt
      841 ttggatagct tttaaaacag gattaagaat ttctgatgta ttgacattac aaaataattg
      901 ccttgcaaaa gttaatggaa agtattcaat aatcacagat attgctaaaa cttttgttaa
      961 aggacataga ataccaattg ataataaatt agctgatata atagcagttt taatagccga
     1021 ttctaaaagt aaaagtacaa aagataataa tcctaataat tatatttttg ctatttataa
     1081 aggaaaaaga aagggaatgc cctttacaca acatatggtc agagcacact taaatcattt
     1141 atcaaagact aaaaatatta ttgatgaaca aggagaaatc ttccatttca aaactcatca
     1201 atttagacat acctatgcag taaaattgtt aaatggtgga gcagatatat taacgataca
     1261 agaattatta gcacactcct caccagaaat gactctaaga tatgctaagt tacttgatga
     1321 tacaaaaaga aaggcttttg aatcagttat tgatcaagga gcttttagtt ttgatgttga
     1381 cggtaaaatt aaaaatatac agcatagtag tgaactatct gaaaaagcat tgaattcttt
     1441 atggcaagaa cataaattaa acgcaatgga caatccttat ggcacttgtc atgctagatt
     1501 gagtggtgat tgcccataca tggaagctcc gccatgcctg acttgtaatt ctgggaaacc
     1561 atgtaaggat ttagctattg gattttctga tttagatgtt gaaaaatatg aattacatat
     1621 caaatcaacg gtcaaatcta tagaattagc taaaaataat aatagacaag atatggttga
     1681 aaagcatata aatatattaa ataaatatga agaaatacta ggtaatataa aagatggaaa
     1741 cattatattc gggagaagta atagaataaa agtatagagg ttaagatgga aaaatttaat
     1801 agacaagaac aattaaaaca gttgcatgca gaacgaaaag ttaaaaccga gaaaaaagtt
     1861 gataaagcaa taaatgattt agttcaaaaa aataaagcaa ttaactttaa catcg
//
LOCUS       DAXWFK010000258         1888 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.262, whole genome shotgun sequence.
ACCESSION   DAXWFK010000258 DAXWFK010000000
VERSION     DAXWFK010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.262"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(83..>1888)
                     /locus_tag="ACGEXS_002618"
     CDS             complement(83..>1888)
                     /locus_tag="ACGEXS_002618"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFF8013570.1"
                     /translation="HFHTHLLYNTCEKYCLGAVFFYFWGVFLYNSLHKCKGFSFHVLL
                     SLTEILKGCLATMDKELWIERANDSLVKHFYEQQSDIEQREGFESKLTFGTAGIRGKF
                     GLGEGRLNKFTIEKLALGLARYLNAQTNSPTIVIHYDIRHLSTEFAQIIANVLANHQI
                     TVYLPDTYKTTPELSFAVRNLNTTAGIMITASHNPKDYNGIKVYGSDGAQLSTDASEL
                     ASRYIEEVGDPLQIDIPISKQNTSYIKPFPKSVTDDYMKHIQNMIGYIPKSDLQVVFT
                     SLHGTSVPIVPELLQSLNFNQFNLVEAQCKPDPNFSSVQSANPEDHRAFDQAVELANK
                     SHADLLISTDPDADRLGIAERDAHGHITYFNGNQIGALLLNYRIQQTSQLRHRLMIQS
                     IVSSELTKSLARYNNVEYKEVLTGFKFIAQEIRQLDDHQNMIFAFEESYGFLSEPFVR
                     DKDAVQIVPLIIKYASELKLYGKTLKDELEQIYQTVGRHEDTLFSHTLEGFEGKKKIN
                     AIMTKFRSNPPQEIQGLKVKAIEDYLTSEVYHLDKDTTSQINSPKSNVIRVLFDEGFI
                     ALRPSGTEPKIKLYVSLKCPNFDDVAQKINAMIFS"
BASE COUNT          621 a          331 c          305 g          631 t
ORIGIN      
        1 cgcgaaaaca taaggattct agtttcaaaa gggctgataa gcataaaatg aaatgtaata
       61 tttcgatgta taaaattttt aattagctaa aaatcatcgc attaattttt tgagctacat
      121 catcaaaatt cggacatttt aacgacacat ataatttaat tttaggttca gtaccagaag
      181 gacgtaaagc gataaatcct tcgtcaaata agacacgaat aacatttgat ttaggagaat
      241 taatctgcga cgttgtatct ttatccaaat gataaacctc gctagttaaa taatcttcaa
      301 ttgctttcac tttgagtcct tgaatctctt gcggtggatt tgaacggaat ttggtcatta
      361 ttgcattaat tttctttttc ccttcaaatc cttctagcgt atgcgagaat aatgtatcct
      421 catgtctacc aacagtttga taaatctgtt ctaattcatc tttcaatgtt ttgccatata
      481 attttaactc agaagcgtat tttataatga gtggcacaat ttgtacggca tctttatcac
      541 gtacaaaagg ctctgataga aaaccgtaac tctcttcaaa tgcgaaaatc atattttgat
      601 gatcatccag ttgtcttatt tcctgagcaa taaatttaaa gcccgtcagc acctctttgt
      661 attcaacatt attatagcgt gctaatgact ttgttaactc actacttaca atagattgaa
      721 tcattaagcg gtgacgtaat tgagacgttt gttgaatacg ataattaagt aacagcgcac
      781 caatttggtt gccattaaaa tatgtgatat gaccgtgtgc atcacgttca gcaataccaa
      841 gtcgatctgc atcagggtca gttgatatta ataagtctgc atgactttta tttgcaagtt
      901 cgactgcttg atcaaaagcg cgatgatctt caggattagc actttgaact gagctgaaat
      961 tcggatcagg tttacattgt gcttctacaa gattaaattg attgaaattc aaagattgta
     1021 ataactcagg tacaatcgga acacttgtac catgcaaact agtgaacaca acttgtaaat
     1081 cagatttagg aatatagcca atcatatttt gaatgtgttt catatagtca tcagttacag
     1141 atttcggaaa aggcttgata taagatgtat tttgtttaga aataggtata tcaatctgta
     1201 atggatcgcc aacttcttcg atataacgac ttgcaagctc agatgcatca gtcgataatt
     1261 gcgcaccatc agaaccatat actttgatgc cgttatagtc tttcggatta tgacttgctg
     1321 taatcataat gccagcagta gtattaagat tgcgcaccgc gaacgataat tccggtgtcg
     1381 ttttatatgt atcaggtaaa taaactgtta tttgatggtt tgctaataca ttagcaatta
     1441 tttgggcgaa ttcagttgaa agatgtctaa tatcataatg aatgactatt gttggactgt
     1501 ttgtttgggc atttaaataa cgcgctaaac ctaatgccaa tttttcaata gtaaacttat
     1561 taagtcgacc ttcaccaaga ccgaattttc cgcgtatacc cgcagtacca aatgttaatt
     1621 tactttcaaa accttctcgc tgttcaatgt cagattgctg ctcataaaaa tgtttaacta
     1681 aactatcatt agctcgttct atccataatt ctttatccat tgttgctaaa catcctttca
     1741 aaatctcagt tagacttaat aaaacatgaa aactaaagcc cttacattta tgtaatgaat
     1801 tataaagaaa tacgccccaa aagtaaaaaa acacagcccc aagacaatac ttttcacaag
     1861 tattatataa tagatgtgta tgaaaatg
//
LOCUS       DAXWFK010000259         1884 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.263, whole genome shotgun sequence.
ACCESSION   DAXWFK010000259 DAXWFK010000000
VERSION     DAXWFK010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.263"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(140..1426)
                     /locus_tag="ACGEXS_002619"
     CDS             complement(140..1426)
                     /locus_tag="ACGEXS_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFF8013571.1"
                     /translation="MNPLAQSLNEQLQQSNATAFTMLSDLGQNMFYPKGILSQSAEAK
                     STTYNATIGMATNKDRKMFASSLDAMFNDLTPDEIFPYAPPQGIEELRDLWQQKMLRD
                     NPELSIDNMSLPIVTNALTHGLSLVGDLFVNQGDTILLPEHNWGNYKLVFNTRNGANL
                     QTYPIFDKDGHYTTDSLVEALQSYNKDKVIMILNYPNNPTGYTPTHKEVTTIVDAIKA
                     LADKGTKVIAVVDDAYYGLFYEDVYTQSLFTALSNLNSNAILPVRLDGATKEFFAWGF
                     RVGFMTFGTSDQTTKEVLEAKVKGLIRSNISSGPLPTQSAVKHVLKNNKQFDKEIEQN
                     IQTLKERYEVTKEVVYADQYHSHWQAYDFNSGYFMAIKVHDVDPEELRKHLIEKYSIG
                     VIALNATDIRIAFSCVEKEDIPHVFDSIAKAIDDLR"
     gene            1626..1724
                     /locus_tag="ACGEXS_002620"
     CDS             1626..1724
                     /locus_tag="ACGEXS_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013572.1"
                     /translation="MMWWQEGMMTLITGGLLIIFRLWLELKWKNKK"
BASE COUNT          605 a          287 c          323 g          669 t
ORIGIN      
        1 tgtataaaac taattaggta tagtgtttga caagttgctg aataaaatat atcttatgat
       61 actttgaaag aaggaataat tttaaaaata aaaaaccaat aatccgagtc atactcatca
      121 gattattggt tgaaattaat tatcttaagt catcaatcgc tttagcaatt gaatcaaata
      181 catgtgggat atcttctttt tctacacagc taaacgcaat acgaatatct gttgcattaa
      241 gtgcaataac accaattgaa tatttttcaa ttaaatgttt acgaagttct tcagggtcaa
      301 catcatgaac ttttattgcc ataaagtatc cagagttaaa gtcataggct tgccaatgtg
      361 aatgatattg atcagcatag acaacttcct tagtgacttc atagcgttct tttaatgttt
      421 gaatattttg ttcgatttct ttatcgaatt gtttattatt ttttaataca tgcttaacag
      481 cgctttgtgt tggaagaggt ccactagaaa tgttacttcg tataagacct tttactttgg
      541 cttctaatac ttctttagtt gtttgatcag acgttccaaa tgtcataaac ccaacacgga
      601 atccccatgc gaaaaattct tttgttgcac catctaaacg aacaggtaat attgcatttg
      661 aatttaaatt agataatgca gtaaataatg attgagtata cacatcttca tagaataaac
      721 catagtatgc gtcatccaca acagctataa cttttgtacc tttatcagct aatgctttaa
      781 ttgcatcgac gatggtagtc acttctttat gcgtaggtgt gtaacctgtc ggattattag
      841 gataatttaa aatcataatg actttatctt tattgtatga ttgtaaagct tctacaagtg
      901 aatcagtagt ataatgcccg tctttatcaa agataggata tgtttgaagg tttgcaccat
      961 ttctcgtatt gaaaacaagt ttgtaattac cccaattatg ctctggtaac aagatagtgt
     1021 caccttgatt tacaaataaa tcgccaacta aagataaacc atgtgttaat gcattcgtaa
     1081 caattggtag tgacatgttg tcgattgata gctctggatt gtcacgcaac attttttgtt
     1141 gccataaatc acgtaattct tcgatgcctt gtggtggcgc ataagggaat atttcatctg
     1201 gagttaaatc attaaacatt gcatctaaag atgatgcaaa catttttctg tctttgtttg
     1261 tcgccatacc tatagttgca ttatatgttg tactctttgc ttcagcagat tgagataaaa
     1321 tgccttttgg ataaaacata ttttgaccta agtcagataa cattgtaaag gcagttgcat
     1381 ttgactgttg aagttgttca tttaagcttt gggctaaagg attcattatt gcttaacctc
     1441 tctttaaaaa aattgtatgc ctatatatta tcttcaatta taccaaagat aaagtgttgt
     1501 tggtgaatta acgaataata tgtaaaatct gctgccaaaa tacgaacata tgttctgttt
     1561 ttatgttaga ataggtgtag gtcatccttc agtatgatac tttagtcgaa cggaggtgta
     1621 gtgtaatgat gtggtggcaa gaagggatga tgacacttat tacaggtggc ttactaatta
     1681 tttttcgact ttggttagaa ctgaaatgga aaaataaaaa atgatcctac acacataaat
     1741 aaccccaagt ctgctggtac cagacttggg gttgtgtagt ataattctgt ggatacactt
     1801 aaataatatc attgaacaca atatatcgca atcaataatc attttgattg aatggttttg
     1861 ttgcgctatt ttagttgaaa gtga
//
LOCUS       DAXWFK010000260         1881 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.264, whole genome shotgun sequence.
ACCESSION   DAXWFK010000260 DAXWFK010000000
VERSION     DAXWFK010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.264"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..1515
                     /locus_tag="ACGEXS_002621"
     CDS             <1..1515
                     /locus_tag="ACGEXS_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HFF8013573.1"
                     /translation="SQQDKKLTGVFGHPVSDRENSMTAGPRGPLLMQDIYFLEQMSQF
                     DREVIPERRMHAKGSGAFGTFTVTKDITKYTNAKIFSEIGKQTEMFARFSTVAGERGA
                     ADAERDIRGFALKFYTEEGNWDLVGNNTPVFFFRDPKLFVSLNRAVKRDPRTNMRDAQ
                     NNWDFWTGLPEALHQVTILMSDRGIPKDLRHMHGFGSHTYSMYNDSGERVWVKFHFRT
                     QQGIENLTDEEAAEIIATDRDSSQRDLFEAIEKGDYPKWTMYIQVMTEEQAKNHKDNP
                     FDLTKVWYHDEYPLIEVGEFELNRNPDNYFMDVEQAAFAPTNIIPGLDFSPDKMLQGR
                     LFSYGDAQRYRLGVNHWQIPVNQPKGVGIENICPFSRDGQMRVVDNNQGGGTHYYPNN
                     HGKFDSQPEYKKPPFPTDGYGYEYNQRQDDDNYFEQPGKLFRLQSEDAKERIFTNTAN
                     AMEGVTDDVKRRHIRHCYKADPEYGKGVAKALGIDINSIDLETENDETYENFEK"
     gene            1606..1755
                     /gene="rpmG"
                     /locus_tag="ACGEXS_002622"
     CDS             1606..1755
                     /gene="rpmG"
                     /locus_tag="ACGEXS_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFF8013574.1"
                     /translation="MRVNVTLACTECGDRNYITTKNKRNNPERIEMKKYCPRLNKYTL
                     HRETK"
BASE COUNT          667 a          286 c          380 g          548 t
ORIGIN      
        1 tcacaacaag acaaaaagtt aactggtgtt tttgggcatc cagtatcaga ccgagaaaat
       61 agtatgacag cagggcctag gggacctctt ttaatgcaag atatttactt tttagagcaa
      121 atgtctcaat ttgatagaga agtaatacca gaacgtcgaa tgcatgccaa aggttctggt
      181 gcatttggga catttactgt aactaaagat ataacaaaat atacgaatgc taaaatattc
      241 tctgaaatag gtaagcaaac cgaaatgttt gcccgtttct ctactgtagc aggagaacgt
      301 ggtgctgctg atgcggagcg tgacattcga ggatttgcgt taaagttcta cactgaagaa
      361 gggaactggg atttagtagg gaataacaca ccagtattct tctttagaga tccaaagtta
      421 tttgttagtt taaatcgtgc ggtgaaacga gatcctagaa caaatatgag agatgcacaa
      481 aataactggg atttctggac gggtcttcca gaagcattgc accaagtaac gatcttaatg
      541 tcagatagag ggattcctaa agatttacgt catatgcatg ggttcggttc tcacacatac
      601 tctatgtata atgattctgg tgaacgtgtt tgggttaaat tccattttag aacgcaacaa
      661 ggtattgaaa acttaactga tgaagaagct gctgaaatta tagctacaga tcgtgattca
      721 tctcaacgcg atttattcga agccattgaa aaaggtgatt atccaaaatg gacaatgtat
      781 attcaagtaa tgactgagga acaagctaaa aaccataaag ataatccatt tgatttaaca
      841 aaagtatggt atcacgatga gtatcctcta attgaagttg gagagtttga attaaataga
      901 aatccagata attactttat ggatgttgaa caagctgcgt ttgcaccaac taatattatt
      961 ccaggattag atttttctcc agacaaaatg ctgcaagggc gtttattctc atatggcgat
     1021 gcgcaaagat atcgattagg agttaatcat tggcagattc ctgtaaacca acctaaaggt
     1081 gttggtattg aaaatatttg tccttttagt agagatggtc aaatgcgcgt agttgacaat
     1141 aaccaaggtg gaggaacaca ttattatcca aataaccatg gtaaatttga ttctcaacct
     1201 gaatataaaa agccaccatt cccaactgat ggatacggct atgaatataa tcaacgtcaa
     1261 gatgatgata attattttga acaaccaggt aaattgttta gattacaatc agaggacgct
     1321 aaagaaagaa tttttacaaa tacagcaaat gcaatggaag gcgtaacgga tgatgttaaa
     1381 cgacgtcata ttcgtcattg ttacaaagct gacccagaat atggtaaagg tgttgcaaaa
     1441 gcattaggta ttgatataaa ttctattgat cttgaaactg aaaatgatga aacatacgaa
     1501 aactttgaaa aataaatttg atatgtagtt tctatattgc gtagttgagc agtttatgat
     1561 atcataataa atcgtaaaga ttcctaacaa gagagggtgt ttaacgtgcg cgtaaacgta
     1621 acattagcat gcacagaatg tggcgatcgt aactatatca ctactaaaaa taaacgtaat
     1681 aatcctgagc gtattgaaat gaaaaaatat tgcccaagat taaacaaata tacgttacat
     1741 cgtgaaacta agtaattctt atcattcaaa tacgacgatt tgaaaataaa gcgggcttac
     1801 ctattatatt ggggagctcg cttttttatg aaatttttgt gaagagtgat taatggattg
     1861 agtttcatcg gtagaacaat a
//
LOCUS       DAXWFK010000261         1878 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.265, whole genome shotgun sequence.
ACCESSION   DAXWFK010000261 DAXWFK010000000
VERSION     DAXWFK010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.265"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            187..>1878
                     /gene="eap"
                     /locus_tag="ACGEXS_002623"
                     /gene_synonym="map"
     CDS             187..>1878
                     /gene="eap"
                     /locus_tag="ACGEXS_002623"
                     /gene_synonym="map"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular adherence protein Eap/Map"
                     /protein_id="HFF8013575.1"
                     /translation="MKFKSLITTTLALGVLASTGANFNNNEASAAAKPLDKSSSSLHH
                     GYSKVHVPYAITVNGTSQNILSSLTFNKNQNISYKDLEDRVKSVLKSDRGISDIDLRL
                     SKQAKYTVYFKNGTKKVIDLKAGIYTADLINTSEIKAININVDTKKQVEDKKKDKANY
                     QVPYTITVNGTSQNILSNLTFNKNQNISYKDLEDKVKSVLESNRGITDVDLRLSKQAK
                     YTVNFKNGTKKVIDLKSGIYTANLINSSDIKSININVDTKKHIENKAKRNYQVPYSIN
                     LNGTSTNILSNLSFSNKPWTNYKNLTSQIKSVLKHDRGISEQDLKYAKKAYYTVYFKN
                     GGKRILQLNSKNYTANLVHAKDVKRIEITVKTGTKAKADRYVPYTIAVNGTSTPILSD
                     LKFTGDPRVGYKDISKKVKSVLKHDRGIGERELKYAKKATYTVHFKNGTKKVININSN
                     ISQLNLLYVQDIKKIDIDVKTGTKAKADSYVPYTIAVNGTSTPILSKLKISNKQLISY
                     KYLNDKVKSVLKSERGISDLDLKFAKQAKYTVYFKNGKKQVVNLKSDIFTPNLFSA"
BASE COUNT          823 a          235 c          275 g          545 t
ORIGIN      
        1 aaaccaaaca acattttagg tattgtaaat taattagtta atcaaaatta aaagaacttt
       61 taacagtgtt aaaaaattat tgttaaagat aatttattat taatattcag ttaattcaaa
      121 aaatagagaa agtctggcta taattaagtt gcaaatacga attacacaaa aaaggagaga
      181 taatttatga aatttaagtc attgattaca acaactttag cattaggtgt tttagcatca
      241 acaggtgcaa actttaataa taatgaagcg tctgccgcag ctaagccatt agataaatca
      301 tcaagttcgt tacaccatgg atattctaaa gtccatgttc catatgcaat cactgtgaac
      361 ggtacaagcc aaaatatttt atcaagctta acatttaata agaatcaaaa tattagttat
      421 aaagatttag aggatagagt taaatcagtt ttaaaatcag acagaggtat tagtgatata
      481 gatttaagac tatcgaagca agcgaaatat actgtttact ttaaaaatgg aacaaagaaa
      541 gttatcgatt tgaaagcagg tatttacaca gctgatttaa ttaatacaag tgaaattaaa
      601 gctattaata ttaacgtaga tactaaaaag caagttgaag ataaaaagaa agataaagca
      661 aattaccaag ttccatacac aatcactgtg aacggtacaa gccaaaatat tttatcaaac
      721 ttaacattta ataagaatca aaatattagt tacaaagatt tagaggataa agttaaatca
      781 gttttagaat caaatagagg tattaccgat gttgatttaa gattatcgaa gcaagcgaaa
      841 tatacagtta attttaaaaa tggaacgaag aaagttatcg atttgaaatc aggtatttac
      901 acagcgaatt taataaattc aagtgatatt aaaagtatca atattaacgt agatacaaaa
      961 aaacatatcg aaaataaagc taaaagaaac tatcaagttc catattcaat taatttaaat
     1021 ggtacatcta caaacatttt atcgaatctt tcattttcaa ataaaccttg gacaaattac
     1081 aaaaatttaa ctagtcaaat aaaatcagta ctgaagcatg atagaggtat tagtgaacaa
     1141 gatttaaaat atgctaagaa agcttattat actgtttatt ttaaaaatgg tggtaaaaga
     1201 atcttacagt tgaattcaaa aaattacaca gcaaacttag ttcatgcgaa agatgttaag
     1261 agaattgaaa ttactgttaa aacaggaact aaagcgaaag cagacagata tgtaccatac
     1321 acaattgcag taaatggcac atcaacacca attttatcag atttaaaatt tacaggtgac
     1381 ccacgtgtag gctacaaaga tatctctaaa aaagttaaat cagtattgaa gcatgataga
     1441 ggtatcgggg aacgtgaatt aaaatatgca aaaaaagcaa cttacacagt acattttaaa
     1501 aatggaacga aaaaagtgat taacataaat tcaaatatta gccaactgaa tctgctttat
     1561 gttcaagata ttaaaaagat agatattgat gttaaaacag gaactaaagc gaaagcggat
     1621 agctatgtac catatacaat tgcagtaaat ggcacatcaa caccaatttt atcaaaactt
     1681 aaaatttcga ataaacaatt aattagttac aaatatttaa atgacaaagt gaaatctgta
     1741 ttaaaaagtg aaagaggcat cagtgatctt gacttaaaat ttgcgaaaca agcaaaatat
     1801 acagtatatt tcaaaaatgg aaagaaacaa gtagtgaatt taaaatcaga catctttaca
     1861 cctaatttat ttagtgcc
//
LOCUS       DAXWFK010000262         1874 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.266, whole genome shotgun sequence.
ACCESSION   DAXWFK010000262 DAXWFK010000000
VERSION     DAXWFK010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.266"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(75..1073)
                     /locus_tag="ACGEXS_002624"
     CDS             complement(75..1073)
                     /locus_tag="ACGEXS_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8013576.1"
                     /translation="MKKVSIKDVAREAGVSVTTVSHILNHNDSRFSATTIKNVHAVSE
                     RLGYAPNKHAKQLRGSKIQTIGVILPSLTNPFFSALMQSIHDHKPSDVDLCFLTSTAT
                     DLYDNIKHLIDRGIDGLIIAQYISSPDALNNYLKKHHVPYVVLDQNDHQGYTDFVRTN
                     EYQGGQLAAQHLIELGHNHMIIVAPYDMMANMSTRVAGFVDTLRANQLPEPQIVHTEL
                     SKRGGLTIVDDIMVQSATAIFAINDELAIGILRGLIEHGISIPKDISLIGYDDIDYAA
                     YVSPPLTTVAQPITDIGKTSLTLLLQRLQHLDKSIDMIELPTTLKIRATTGYHLSN"
     gene            1224..1403
                     /locus_tag="ACGEXS_002625"
     CDS             1224..1403
                     /locus_tag="ACGEXS_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFF8013577.1"
                     /translation="MRRENILLRLIPFNDYDNKNFKMFIVIKNTKVIILKNKIFVFEQ
                     SIKKHNEMMYIELCD"
     gene            1453..1845
                     /locus_tag="ACGEXS_002626"
     CDS             1453..1845
                     /locus_tag="ACGEXS_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013578.1"
                     /translation="MQFKLKEEEIISFLELKYPEKEFEYGRLLVGQHKRDDLDVYYFG
                     DTFLMCTIISFKTFEIKETVELSYDAVNRIVLKDGWLFRKMRIETMQKVLKYGTSKLM
                     LTDFQKENYNKYIQGQKQRVIFENGHFV"
BASE COUNT          645 a          250 c          351 g          628 t
ORIGIN      
        1 atgattaaaa accctcagca ttaaagcaac gctaagggcc taacaatgat gagtatattt
       61 cggaagatac gtagttagtt tgaaagatga tagccagttg ttgcacgaat ttttaaagtc
      121 gttggtaatt caatcatatc aatggattta tctaagtgct gtaatcgttg aagtaataag
      181 gttaaagatg ttttgccaat atcagttata ggttgtgcca cagtagttaa aggtggcgag
      241 acgtacgctg cataatcaat gtcgtcataa cctattaatg agatatcttt cgggatactg
      301 atgccatgtt caattagtcc tcgtaaaatg ccaatagcga gttcatcgtt aatagcgaag
      361 attgcagtgg cagattgaac catgatgtca tcaacaatgg ttagcccacc gcgcttagat
      421 aattcagtat ggacgatttg tggttctggc aattgattcg cgcgcaaagt atcgacaaat
      481 ccagcgacac gagtcgacat attcgccatc atgtcatatg gtgcaacaat tatcatatgg
      541 ttgtgaccga gttctattaa atgttgtgct gcaagttgtc caccttgata ttcatttgtc
      601 cgaacaaaat ctgtatagcc ttgatggtca ttttgatcca gtacgacata aggtacatga
      661 tgtttcttta gatagttatt tagggcgtcc ggggatgata tgtattgtgc gataattaat
      721 ccgtcaatac ctcgatcaat taaatgttta atattgtcat acaaatcagt tgctgtagat
      781 gttaaaaagc ataaatcaac atcagatggt ttatggtcat gaatactttg catcagtgct
      841 gagaaaaacg gatttgttaa gctaggcaaa atgacgccaa tagtttgaat tttactgccg
      901 cgcaattgtt ttgcatgttt attaggggca tagcctaaac gttctgaaac agcatgtacg
      961 ttttttatcg ttgttgcgga aaaacgacta tcattatgat ttaaaatatg tgacacagtt
     1021 gtaactgata caccagcttc tctagcaaca tctttaattg acactttttt catattatat
     1081 tccttctctt tattgtgtaa ttaatcatac agttatatac ccgattatta tattcatcat
     1141 atcatgataa acatttgatg acatatgaag acgtgaatga cttttattgt ctacaaacag
     1201 acgtctgaag tatacgatta aggttgagac gagagaatat attattaagg ttaatacctt
     1261 ttaatgatta tgataacaag aattttaaaa tgtttattgt gattaaaaat acaaaagtca
     1321 ttattttaaa gaataaaata tttgtttttg aacaaagtat taaaaaacat aacgaaatga
     1381 tgtatattga actatgtgat tgaaaaaatt tttaattaag gcttgttaaa acttaagaga
     1441 ggatgttttt aaatgcaatt caaattaaaa gaagaagaga ttattagttt tttagaattg
     1501 aaatatccag aaaaagagtt cgaatatggt cgtttgttag ttggacaaca taaacgtgat
     1561 gatttagatg tttattactt tggtgatacg tttttaatgt gcacgattat ttcattcaag
     1621 acatttgaaa ttaaagaaac agtagaatta tcatatgatg ctgttaatcg tattgtgtta
     1681 aaagatggat ggttatttag aaaaatgaga atagaaacaa tgcaaaaagt gttaaaatac
     1741 ggtacatcta aattaatgtt aactgatttt caaaaagaga attataataa atatattcaa
     1801 ggtcagaaac aacgcgtgat atttgaaaat ggccattttg tctaattgat agtgaatata
     1861 attagagtaa gagg
//
LOCUS       DAXWFK010000263         1851 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.267, whole genome shotgun sequence.
ACCESSION   DAXWFK010000263 DAXWFK010000000
VERSION     DAXWFK010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.267"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..464)
                     /locus_tag="ACGEXS_002627"
     CDS             complement(<1..464)
                     /locus_tag="ACGEXS_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS257 family transposase"
                     /protein_id="HFF8013579.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRERGVNVHH
                     STVYRWVQEYAPVLYQIWKKKHKKAYYKWRVDETYIKIKGQWCYLYRAIDADGHTLDI
                     WLRKQRDNHSAYAFIKRLIKQFGKPQKVITDQAPSTKVAMAKVIKAFKLKPD"
     gene            complement(735..830)
                     /locus_tag="ACGEXS_002628"
     CDS             complement(735..830)
                     /locus_tag="ACGEXS_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFF8013580.1"
                     /translation="MLEILVHITTTVISGCIIALFTHWLRNRNNK"
     gene            995..1668
                     /locus_tag="ACGEXS_002629"
     CDS             join(995..1601,1601..1668)
                     /locus_tag="ACGEXS_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015311.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFF8013581.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRERGVNVHH
                     STVYRWVQEYAPVLYQIWKKKHKKAYYKWRVDETYIKIKGQWCYLYRAIDADGHTLDI
                     WLRKKRDHQSAYAFIKRLIKQFGKPQMIITDQAPSTKVAMAKIIKVFKLKPNCHCTSK
                     YLNNFIEQDHRHIKVRKTRYQSVNTAKNTLKGIECIYGLYKKNRRSLQIYGFSPCHEI
                     SYMLAG"
     gene            1729..1851
                     /locus_tag="ACGEXS_002630"
                     /pseudo
     CDS             1729..1851
                     /locus_tag="ACGEXS_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          628 a          323 c          323 g          577 t
ORIGIN      
        1 cagtccggtt taagtttaaa agctttaatt actttagcca ttgctacctt cgttgaaggt
       61 gcctgatctg taattacctt ttgaggttta ccaaattgtt taatgagacg tttgataaac
      121 gcatatgctg aatgattatc tcgttgctta cgcaaccaaa tatctaatgt atgtccatct
      181 gcatcaatcg cgcgatacag ataacaccac tgtcctttaa ttttgatata tgtctcatca
      241 acacgccact tataatacgc ttttttatgt tttttcttcc aaatttgata caaaacggga
      301 gcatattctt gaacccaacg gtagaccgtt gaatgatgaa cgtttacacc acgttccctt
      361 aatatttcag atatatcacg ataactcaat gcatatctta gatagtagcc aaccgctaca
      421 gtgataacat ccttgttaaa ttgtttatat ctgaaatagt tcatacagaa gactcctttt
      481 tgttaaaatt atactataaa ttcaactttg caacagaacc agttaaacaa tgttatgcca
      541 agttacaatg tttttatccg taaagacgag ttagattata gatagaaata atattcaaaa
      601 gagaaatgaa tgaggacttt gcatttctcc actcaccgat gttataattt agttaacaag
      661 gaaactagca tagatgcacc aatcccctca ctacggcaat agtgagggga ttttttaggt
      721 gtggctatgg tcgcctattt attattgcga ttacgtagcc aatgcgtaaa taacgcaata
      781 atacaaccac tgatgactgt ggtcgtgatg tgaacaagga tttctagcat agattagcac
      841 ctcctctctg cgtcggaatt gacgcctgag agatagacga cttgtatatt atatcatcta
      901 cgaatatacg atgataggta gcttctgata agtgatctgg ttctgttgca aagttgattt
      961 tgtagtataa ttttaacaaa aaggagtatg ctatatgaac tatttcagat ataaacaatt
     1021 taacaaggac gtcatcactg tagccgttgg ctactatcta agatatgcgc ttagttatcg
     1081 tgatatatct gaaatactaa gagaacgtgg tgtcaacgtt catcattcaa cggtctaccg
     1141 ttgggttcaa gaatatgctc ccgttttgta tcaaatttgg aagaaaaaac ataaaaaagc
     1201 gtattataag tggcgtgttg atgagacata tatcaaaatt aaaggacagt ggtgttatct
     1261 gtatcgcgcg attgatgcag atggacatac attagatatt tggttacgca aaaaacgaga
     1321 tcatcaatca gcatatgcgt ttattaagcg ccttattaaa caattcggta aacctcaaat
     1381 gataattaca gatcaagcac cttcaacgaa ggtagccatg gctaaaataa ttaaagtgtt
     1441 taagttgaaa cctaactgtc attgcacatc taaatatctc aataacttca ttgaacaaga
     1501 tcatcgtcac attaaggtga gaaagacaag atatcaaagt gtcaatacgg caaaaaatac
     1561 actcaaaggc attgaatgta tttacggatt atataaaaaa accgtaggtc tcttcagatc
     1621 tacggatttt caccatgcca cgaaattagt tatatgttag ctggttaggc gaagactgac
     1681 acaataaaat ggtgattagc tatatttttc taactttgca acagaacccc taaaaaaaat
     1741 tcaaaatctt tcagagaagc tgttaaagga atttttaagt tcacagatat taacttagcg
     1801 cgtgaggcta aaaatcgatt gattcatgat tatatcgatc aaccaaaata t
//
LOCUS       DAXWFK010000264         1820 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.268, whole genome shotgun sequence.
ACCESSION   DAXWFK010000264 DAXWFK010000000
VERSION     DAXWFK010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.268"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(252..1187)
                     /gene="trxB"
                     /locus_tag="ACGEXS_002631"
     CDS             complement(252..1187)
                     /gene="trxB"
                     /locus_tag="ACGEXS_002631"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFF8013582.1"
                     /translation="MTEIDFDIAIIGAGPAGMTAAVYASRANLKTVMIERGIPGGQMA
                     NTEEVENFPGFEMITGPDLSTKMFEHAKKFGAVYQYGDIKSVEDKGEYKVINFGNKEL
                     TAKAVIIATGAEYKKIGVPGEQELGGRGVSYCAVCDGAFFKNKRLFVIGGGDSAVEEG
                     TFLTKFADKVTIVHRRDELRAQRILQDRAFKNDKIDFIWSHTLKSINEKDGKVGSVTL
                     TSTKDGSEETHEADGVFIYIGMKPLTAPFKDLGITNDVGYIVTKDDMTTSVPGIFAAG
                     DVRDKGLRQIVTATGDGSIAAQSAAEYIEHLNDQA"
     gene            complement(1254..>1820)
                     /locus_tag="ACGEXS_002632"
     CDS             complement(1254..>1820)
                     /locus_tag="ACGEXS_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013583.1"
                     /translation="LGEEDESHYYWDKLKQISKVEIGHAPWVIENSKEVFDQHILPLL
                     QSDDSHYRLYGIFLLDQLNGKEIVMTESIWQVLENLNNYEKLYLTYLVQGLTLNKLDF
                     IHRGLLTLYHNELFVSENDVMVAWINQGELIIAEKVDLTDVEPYIGAFIYLYFKNQPR
                     NVTKKQITTWLGITQYKLNKMIEFLLSI"
BASE COUNT          577 a          373 c          230 g          640 t
ORIGIN      
        1 taagaaccac tacataataa atcattagtg gctctttatc atttctgtcc cactcccata
       61 aagttataag tttaatataa catataaaaa aactagaact cattgaaacg cacaaatgat
      121 acgtactaag ttctagtttt ctatataatt tgctttaatt taaaagtaag acattatttt
      181 aacgctgtga tgacactatt aaaataaata tccaaataat ttacagctca acatcttaat
      241 tcgacttcga attaagcttg atcgtttaaa tgttcaatat attccgctgc actttgcgct
      301 gcaatactac catcgccagt agcagtgaca atttggcgta aacctttgtc gcgaacatct
      361 cctgctgcaa aaatacctgg tactgatgtt gtcatatcat cttttgttac aatataacca
      421 acatcatttg taatacctaa gtctttaaat ggcgctgtta atggtttcat accaatatag
      481 atgaatacac catcagcctc gtgtgtttct tctgaaccat cttttgtaga cgttaatgtc
      541 acagaaccca ctttgccgtc tttttcatta attgatttca aagtatgact ccaaataaag
      601 tcgattttat catttttgaa tgctctatct tgtaaaatac gctgtgcacg taactcatca
      661 cgacggtgaa cgattgttac tttgtcagca aatttagtta agaatgttcc ctcttctact
      721 gctgaatcac caccaccgat aacgaatagg cgtttatttt taaagaatgc accatcacat
      781 actgcacaat aacttacacc gcgtccacca agttcttgtt cacccggaac accaattttc
      841 ttgtattctg cacctgtagc aataataacc gctttcgctg ttaattcttt attaccaaag
      901 ttaatcactt tatattcgcc tttatcttct acagatttaa tatctccata ttgataaact
      961 gcaccaaact ttttagcgtg ttcaaacatt tttgtagata aatctggacc tgtaatcatt
     1021 tcgaaaccag ggaagttctc tacttcttct gtattagcca tttgaccgcc tggaatacct
     1081 ctttcaatca taactgtttt taaattagca cgtgatgcgt atactgcagc agtcatacca
     1141 gctggacctg caccgataat tgctatatca aaatctattt cagtcatttt attaacgcct
     1201 cctcattatt aatcattatg cgcattatat aataaatcta acttttcata aatctatatg
     1261 ctcaagagaa attcaatcat tttgttcagt ttatattgtg ttatgcctaa ccatgttgta
     1321 atttgcttct ttgtaacgtt tcgaggttga tttttaaaat acaaataaat aaacgcaccg
     1381 atatatggct caacatcagt taaatctact ttttcagcaa ttatgagttc accttgatta
     1441 atccatgcaa ccattacatc attttcactt acaaataatt cattatggta aagcgttaat
     1501 aagccgcgat gaatgaagtc taatttattg agcgttaaac cttgaactaa atacgttaaa
     1561 tacaatttct cataattatt tagattttcc aaaacctgcc aaatactttc cgtcatcaca
     1621 atttctttac catttaattg atccaataaa aaaataccat ataaacgata atgactgtca
     1681 tcactttgaa gtaatggcaa aatatgttgg tcaaaaactt ctttgctatt ttcaattacc
     1741 caaggcgcat gtccaatttc cactttagaa atttgcttca atttatccca gtagtaatga
     1801 ctttcgtctt cttcacctaa
//
LOCUS       DAXWFK010000265         1806 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.269, whole genome shotgun sequence.
ACCESSION   DAXWFK010000265 DAXWFK010000000
VERSION     DAXWFK010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.269"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..907
                     /locus_tag="ACGEXS_002633"
     CDS             <1..907
                     /locus_tag="ACGEXS_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron-hydroxamate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFF8013584.1"
                     /translation="RLIGILLCNLFILTACSASVDKTSNSTKTTDYKIENGETLKVPE
                     KPKRVAVLTGFYVGDFIKLGIKPIAVSDITKDSSILKPYLKGVDYIGENDVERVAKAK
                     PDLIVVDAMDKNIKKYQKIAPTIPYTYNKYNHKEILKEIGKLTNNEDKAKKWIEEWDD
                     KTRKDKKEIQSKIGQATASVFEPDEKQIYIYNSTWGRGLDIVHDAFGMPMTKQYKDKL
                     QEDKKGYASISKENISKYAGDYIFLSKPSYGKFDFEKTHTWQNIEAVKKGHVISYKAE
                     DYWFTDPITLEHLRSKLKKEILNKK"
     gene            969..>1806
                     /locus_tag="ACGEXS_002634"
     CDS             969..>1806
                     /locus_tag="ACGEXS_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8013585.1"
                     /translation="MKQLVGIPESMLIPLIARAKEYENEKPIIKDALSKKIFDGLDDM
                     YKNVTCDDMSQIGISIRTVIIDCVTKRLIKDNKDLIVVNIGCGLDTRFQRFNKEKISW
                     IDLDVPESIEIRKTFFKESNSYKMISKSMLDYSWIDDVKNYNFFNSKSDILFIIEGVL
                     MYFDESVMTQLLDTIIKKMGDHNLTFAIEFCSKTIANNTKRHQSVSKLSSQPVFKYGY
                     NDLKKLNEILPNTIRVIHEYNYFDYYKNRWGLFGYCRFIPYLKKRLNNKIVLMKYKVP
                     KRQ"
BASE COUNT          761 a          186 c          303 g          556 t
ORIGIN      
        1 aagattaatt ggaattttat tatgtaattt atttattttg actgcgtgtt cagcatctgt
       61 cgataaaaca agtaactcga caaagactac agactataaa attgaaaatg gtgaaacact
      121 gaaagtacca gagaaaccta aaagagttgc tgtattaact ggattttatg ttggtgattt
      181 tataaagtta ggaatcaaac caattgctgt ttcagatata actaaagatt cttcaatttt
      241 aaaaccttat ttaaaagggg ttgattatat tggagaaaat gatgttgaaa gagttgctaa
      301 agcaaaacca gatttaattg ttgtagatgc tatggataaa aatataaaaa aataccaaaa
      361 aatagcgcca acaatcccat atacatacaa taaatacaat cataaagaaa tattaaaaga
      421 aataggcaag ttgactaata atgaagataa agctaaaaaa tggattgaag agtgggacga
      481 taaaactaga aaagataaaa aagaaattca aagtaaaatt ggtcaagcaa cagcatctgt
      541 gtttgaacca gatgaaaagc aaatatacat atataactct acatggggtc gtggtttgga
      601 tattgttcat gatgcattcg gtatgccaat gacaaagcaa tataaagata aattacaaga
      661 agataaaaaa ggctatgctt ccatttcaaa agaaaatatt agtaaatatg ctggtgatta
      721 tatattttta agtaaacctt cgtacggaaa atttgatttt gaaaaaacac atacatggca
      781 gaatattgaa gctgtaaaaa aaggacatgt aatttcatat aaagcagaag attattggtt
      841 cacagatcct attacattag aacatttgag aagtaaatta aaaaaagaaa ttttaaataa
      901 aaaataatag aaataagttg taaaaatatt cttatgcatt ggtactaatg tttttaagga
      961 gtgattaaat gaagcaactg gttggaattc ccgaatcaat gttaattcct ttgatagctc
     1021 gagcaaaaga gtacgaaaac gaaaaaccaa taataaaaga cgcactatca aaaaaaatat
     1081 ttgatggttt agatgatatg tacaaaaatg ttacatgtga tgacatgtct caaattggaa
     1141 ttagtatacg tactgtgata atagattgtg ttactaagag gcttatcaag gataataaag
     1201 atttaatcgt ggtcaatata ggttgtggct tagatacaag gtttcaaaga tttaataaag
     1261 aaaaaatatc atggatagat ttagatgtac cagaatcaat agaaatacga aaaacatttt
     1321 ttaaagaaag taatagttat aagatgatat ctaaatctat gctagattac agttggattg
     1381 atgatgtcaa aaattataat ttttttaata gtaagtcaga tatattgttt attattgaag
     1441 gtgtattgat gtattttgat gagagtgtaa tgactcaatt attggacact attatcaaaa
     1501 agatgggaga tcataatttg acatttgcga ttgaattttg ctcaaaaaca attgcgaata
     1561 atacaaagag acatcaatcg gtatcaaagt tatcctcaca acctgttttt aaatatggtt
     1621 ataatgattt aaaaaaattg aatgaaattt taccaaatac aataagagta atacatgaat
     1681 ataattattt tgactattat aagaatcgat ggggcttatt tggatattgt agatttatac
     1741 cttatttaaa aaaaaggtta aacaataaaa tagtacttat gaaatataaa gttccaaaga
     1801 gacaga
//
LOCUS       DAXWFK010000266         1797 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.270, whole genome shotgun sequence.
ACCESSION   DAXWFK010000266 DAXWFK010000000
VERSION     DAXWFK010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.270"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(1..950)
                     /locus_tag="ACGEXS_002635"
                     /pseudo
     CDS             complement(1..950)
                     /locus_tag="ACGEXS_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500433.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            complement(943..>1797)
                     /locus_tag="ACGEXS_002636"
     CDS             complement(943..>1797)
                     /locus_tag="ACGEXS_002636"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFF8013586.1"
                     /translation="GSLLLRVGKETQVYRYFGQERNNTTYNLARMNMLLHDVRYENFE
                     IRNDDTLENPAFLGNTFDAVIANPPYSAKWTADSKFENDERFSGYGKLAPKSKADFAF
                     IQHMVHYLDDEGTMAVVLPHGVLFRGAAEGVIRRYLIEEKNYLEAVIGLPANIFYGTS
                     IPTCILVFKKCRQQDDNVLFIDASNDFEKGKNQNHLSDAQVERIIDTYKRKATIDKYS
                     YSATLQEIADNDYNLNIPRYVDTFEEEAPIDLDQVQQYLKNIDKEIAEIEQEINAYLK
                     ELGVLKDE"
BASE COUNT          477 a          353 c          272 g          695 t
ORIGIN      
        1 atgatcatta cttttaacta catgcgcatg attatttacc cagtattgcc cattagcaat
       61 aaagctactc gtctcaaact gcccccattt tgcaccatct tctcctatta gtaatcgttc
      121 ttcattattg aataaataat cttttacgta atcaataatt ccagttgcac catagtaagg
      181 gtataacccc ttttctctta atgaagaagt aattggtttt cttctattat tttcaaagat
      241 aaagatgtct tttatgaatt tgttttccca ctctggatat tcttcaccat tctcatcttt
      301 gaatcgcaat tcttgtgtga agattttctg catatagcct tttttctgtt gttgaagcaa
      361 ttcaagtttt tgttcttcta attcaatttg tcggtcgagt ttgctgaaga acttgcctat
      421 tttttgctgt tcttcaaata tagttggggt gaagattttt aaattagcaa tttctttgaa
      481 gtttagacct tctcgactac ctccactttg tgcaaggaaa attttccttt taccttttct
      541 tgataataga tactgtccaa aaaaattata ataatactct tttttcaatc taataataca
      601 tgttgattta aattagcatg cgtttcaact atcgaattaa tggctgttct acctattgat
      661 gctcctgtaa tatttaaaag aacatcacca tagtacgttc tactattttt catctcatca
      721 tctatatctt tactaatata aactaagtca ttaagattta atttaccatt tctaatattt
      781 tgactcctta aaaatggtat gcctttgttt gtatagtttt cacttccacc tttgggagtc
      841 tttccactac ctattttggt agtaagattc cctaacttct tctcttccca ttcgccttca
      901 aatcctggga atctcaactc tggcacattt ttcgtttgtg tattactcat ctttcaacac
      961 cccaagttct ttcaggtatg cattgatttc ttgttcaatt tctgcgattt ctttgtcgat
     1021 atttttcaaa tattgttgga cttgatctaa atcaattggt gcttcttctt cgaatgtatc
     1081 aacatatcgc ggtatgttta agttgtaatc gttatcggcg atctcttgta atgtcgcgct
     1141 gtagctatat ttatcaatcg ttgctttacg cttatatgtg tctataatac gttcgacttg
     1201 ggcatcgctt aaatggtttt gattttttcc tttttcaaaa tcattggatg catcgataaa
     1261 tagtacgttg tcgtcttgtt ggcgacattt tttaaatact aaaatacatg ttggaatact
     1321 tgtcccatag aaaatattgg ctggtaaccc aatcacggct tctaagtagt tcttttcttc
     1381 tattaaatag cgacgaatca caccttctgc ggcaccacgg aataatacac catgtgggag
     1441 tacgactgcc atggtacctt catcgtctag gtaatgtacc atgtgttgaa taaaggcaaa
     1501 gtctgctttg gattttggcg caagcttgcc gtaaccactg aatcgttcat cattttcaaa
     1561 ttttgaatct gctgtccatt tcgcactgta tggtgggttc gcaataaccg catcaaatgt
     1621 attgcctaaa aaggctggat tttccaatgt gtcatcatta cggatctcga agttctcata
     1681 acgcacatca tgtaataaca tattcatgcg tgctaagttg tatgtagtat tgttacgttc
     1741 ttgtccgaaa taacgataca cttgcgtttc tttaccaaca cgtaacaaca atgaacc
//
LOCUS       DAXWFK010000267         1791 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.271, whole genome shotgun sequence.
ACCESSION   DAXWFK010000267 DAXWFK010000000
VERSION     DAXWFK010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.271"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..322
                     /locus_tag="ACGEXS_002637"
     CDS             <1..322
                     /locus_tag="ACGEXS_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFF8013587.1"
                     /translation="GNASQREELAKQLSQNGGKVSLDMLQTTMGALAIILLISTLYGI
                     FATICIKGRRKLSIILFVIAIIVSLMALNLIAIVLWVIVMIMLISKKESKETTHKDDE
                     YIYH"
     gene            complement(404..1756)
                     /locus_tag="ACGEXS_002638"
     CDS             complement(404..1756)
                     /locus_tag="ACGEXS_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFF8013588.1"
                     /translation="MNNKRHSTNEQLSLDEINNTIKFDHRSSNKQKFLSFLGPGLLVA
                     VGYMDPGNWITSMQGGAQYGYTLLFVILISSLSAMLLQSMTVRLGIATGMDLAQMTRH
                     YLSRPIAIIFWIIAELAIIATDIAEVIGSAIALNLLFNIPLIVGALITVLDVFLLLFI
                     MKYGFRKIEAIVGTLIFTVLFIFIFEVYISSPQLNAVLNGFIPHSEIITNNGILYIAL
                     GIIGATIMPHNLYLHSSIVQSRTYSRHNNEEKAQAIKFATIDSNIQLSIAFVVNCLLL
                     VLGASLFFNSNADDLGGFYDLYHALKTEPVLGATMGAIMSTLFAVALLASGQNSTITG
                     TLAGQIVMEGFLRLHIPNWLRRLITRSLAVIPVIVCLIIFKGNAAKIEQLLVFSQVFL
                     SIALPFCLIPLQLATSNKDLMGPFYNKTWVNIISWTLIIILSILNVYLIVQTFQELQS
                     "
BASE COUNT          630 a          268 c          291 g          602 t
ORIGIN      
        1 tggtaatgca tcacaacgtg aagaattagc gaagcaatta tctcagaacg gtggcaaggt
       61 ttctttagat atgcttcaga caacaatggg tgcattagca attattttat taatttcaac
      121 actttatggt atatttgcga caatttgtat taaaggacgt agaaaattat cgattatact
      181 ttttgttatc gcgataattg taagtttgat ggctcttaat ttaattgcaa ttgtcttatg
      241 ggttatcgtg atgattatgt tgatttctaa aaaagaatca aaagaaacaa cacataagga
      301 cgatgagtat atttatcatt aatatgttca tagcaaaaga aaaaccatta aaatgttaag
      361 ttgttaatta ttagatacaa ccaacatttt aatggtttta tttttaactt tgtagttctt
      421 ggaatgtttg aacgattaaa tagacattta aaatacttag aataataata agtgtccatg
      481 aaatgatatt aacccatgtt ttattataga aaggtcccat caagtcttta ttactagtag
      541 ccaattgtaa tggtattaaa cagaatggca gagcaatact taaaaatact tgagagaata
      601 caagtagttg ctcaatttta gctgcattac ctttaaaaat gattaagcat acgataacag
      661 gaatgacagc aagagaacgt gtaattaaac gtcttaacca atttggtatg tgtaatctta
      721 aaaatccttc cattacaatt tgtcctgcta aagtaccagt aatcgttgaa ttttgacctg
      781 acgctaataa tgcaactgca aataatgtac tcatgattgc acccattgtt gcacctagta
      841 caggttcagt ttttaaggcg tgatataaat catagaaacc acctaaatcg tcagcatttg
      901 agttgaaaaa tagtgatgct cctaacacta ataataagca attgactaca aatgcgattg
      961 ataactgaat gttcgaatct atcgtagcaa atttaatcgc ttgcgctttt tcttcattgt
     1021 tatgtcttga gtatgttcta gattgtacaa ttgatgaatg taagtacaaa ttatgaggca
     1081 taattgtagc gccaataata cctaatgcaa tatagagaat gccgttattt gtaatgattt
     1141 cactatgtgg tataaatcca tttaacacag cattcaactg tggtgatgaa atatagactt
     1201 caaatataaa gatgaataac actgtgaaaa ttaatgtacc aacaatagct tcaatttttc
     1261 taaaaccata tttcattata aaaagtagta aaaatacatc aagtacagtt attagtgcac
     1321 cgacgattaa aggtatgtta aataggagat taagagcaat agcactacca ataacctcag
     1381 caatatctgt agcgataatt gctagttctg caatgatcca aaagattata gcaataggtc
     1441 ttgataaata atgtcgtgtc atttgagcta agtccatacc tgttgctatt cctaatctca
     1501 ctgtcatgct ttgaagtaac attgctgata aacttgaaat aagaattacg aatagcaaag
     1561 tatagccata ttgggcgcca ccttgcattg atgttatcca gtttccggga tccatgtaac
     1621 caacagcgac taataaccca ggtccaagaa atgataaaaa tttctgttta tttgaactgc
     1681 gatgatcgaa tttaattgta ttgtttattt cgtctaaact taattgttca tttgttgaat
     1741 gtcgtttatt attcattttg actcacctct acttcttatg gacaatataa t
//
LOCUS       DAXWFK010000268         1785 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.272, whole genome shotgun sequence.
ACCESSION   DAXWFK010000268 DAXWFK010000000
VERSION     DAXWFK010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.272"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..520
                     /gene="lpl2"
                     /locus_tag="ACGEXS_002639"
     CDS             <1..520
                     /gene="lpl2"
                     /locus_tag="ACGEXS_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl2"
                     /protein_id="HFF8013589.1"
                     /translation="LDNEGMILNLDRNTRTAKGYYFVDTIYDNHENSYSKNYRVEMKN
                     NKIILLDKVEDQKLKERIENFKFFGQYADFKSLKSYNNGDVSINSNVPSYDAKFKMSN
                     KDENVKQLRSRYNIPTEKAPILKMHIDGDLKGSSVGYKKLEIDFSKEENSELSVVDSL
                     NFQPAKKNKDDE"
     gene            539..1346
                     /locus_tag="ACGEXS_002640"
                     /pseudo
     CDS             539..1346
                     /locus_tag="ACGEXS_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000269700.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
     gene            1392..>1785
                     /gene="lpl4"
                     /locus_tag="ACGEXS_002641"
     CDS             1392..>1785
                     /gene="lpl4"
                     /locus_tag="ACGEXS_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl4"
                     /protein_id="HFF8013590.1"
                     /translation="MMGYLKRLVLYIVIMVMSVFIIGCDKSSDTAEKSKEDSKETQIK
                     KSFAKTLDMYSIKNLEELYDKEGYRDGEFEKGDKGMWTIYTDFAKSNKPGELSNEGMV
                     LYLDRNTRTAKGYYFVRTFYRKDKLPDRK"
BASE COUNT          759 a          184 c          352 g          490 t
ORIGIN      
        1 attagataat gaaggtatga ttttaaattt ggatagaaat acacgtacgg ccaagggata
       61 ttattttgta gatactatat atgacaatca tgaaaactct tatagtaaaa attatagagt
      121 tgagatgaaa aacaataaaa ttattttatt agacaaggtg gaagatcaaa aacttaaaga
      181 aagaatagaa aactttaaat ttttcggaca atatgccgat ttcaagagtt tgaaaagtta
      241 caacaatggc gacgtttcaa ttaatagtaa tgttccaagt tatgacgcga aatttaaaat
      301 gagtaataaa gatgaaaatg ttaagcaatt aagaagtcgt tataacattc ctactgaaaa
      361 agctccaata ttaaaaatgc atattgatgg ggacttaaaa ggcagttccg ttggatataa
      421 aaagttagaa atagactttt caaaagaaga aaatagcgaa ttatcagtag tcgattcatt
      481 aaattttcag cctgccaaaa aaaataaaga tgatgaatga tagggaggat gtgaaacaat
      541 gtggtcaata aaaagaattg gaatgtacac aaatgttata attttgagcg tttttataat
      601 aggttgtgat agttcaagca ataatgcaga aaatcaaaga gaagactcaa aagaagaaca
      661 aatcaaaaag agctttgcga aaacgttaga tatgtaccct attaagaatc tcgaggattt
      721 atatgacaaa gaaggatatc gtgatggtga gtttgaaaaa ggcgataagg ggacgtgggt
      781 attgtattca gctatggtat cacaaccaaa aggcgagagt ttaaaatcac gaggaatgat
      841 attaaagtta gatagaaata agagaactgc taaaggaagt tatattatta gagaattgaa
      901 agaagataaa aatcatgatg ttcaaaaaaa tgaaaagaaa tatccagtga aattggtgaa
      961 taataggata gttttgataa aagatgttaa agacaaaaag ttaaaaaaat gaaatagagt
     1021 cgtttgaatt attttcacaa tatggaaact ttaatcattt tgatcggaat gagattacta
     1081 atatttcata taatcctaat gctcccaatt actctgcaga atataaaatg aagaaaaatg
     1141 acagaaacat tcaacagttg aaaaagagat ttaatctaaa aactagcaag acaccaaaat
     1201 tattgtttaa gggatctgga gatataaagg ggtcttctgt aggatataag gaaatagaaa
     1261 tcatatttag tagaagtaaa gaagaaagca tttattatgt tgacagcgtt gagttcgttc
     1321 caagtaacaa aataacaaaa agttgatatt agtcaacaaa agcaaatatt gagaataaag
     1381 agagttgtga aatgatgggt tatttaaaaa gacttgtatt gtatatagtt attatggtta
     1441 tgagtgtttt tataataggt tgtgataaat caagcgatac tgcagaaaaa tcaaaagaag
     1501 actcgaaaga aacacaaatc aaaaagagtt ttgcgaaaac gttagatatg tattcaatta
     1561 agaatctcga ggaactatat gacaaagaag gctatcgaga tggcgaattt gaaaaaggtg
     1621 acaaagggat gtggacgata tatacagatt tcgccaaaag taataaaccg ggtgaactaa
     1681 gtaatgaagg catggtcttg tacttagata gaaacacacg cacagctaag gggtactact
     1741 ttgtcaggac gttttatcgc aaggataaat tacctgatag aaaaa
//
LOCUS       DAXWFK010000269         1765 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.273, whole genome shotgun sequence.
ACCESSION   DAXWFK010000269 DAXWFK010000000
VERSION     DAXWFK010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.273"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(298..633)
                     /locus_tag="ACGEXS_002642"
                     /pseudo
     CDS             complement(298..633)
                     /locus_tag="ACGEXS_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500032.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
     gene            complement(1284..>1765)
                     /gene="sak"
                     /locus_tag="ACGEXS_002643"
     CDS             complement(1284..>1765)
                     /gene="sak"
                     /locus_tag="ACGEXS_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="staphylokinase"
                     /protein_id="HFF8013591.1"
                     /translation="SLLFLTVLLLLFSFSSITNEVSASSSFDKGKYKKGDDASYFEPT
                     GPYLMVNVTGVDGKGNELLSPHYVEFPIKPGTTLTKEKIEYYVEWALDATAYKEFRVV
                     ELDPSAKIEVTYYDKNKKKEETKSFPITEKGFVVPDLSEHIKNPGFNLITKVVIEKK"
BASE COUNT          604 a          320 c          264 g          577 t
ORIGIN      
        1 gatttggaaa gcgcaaaaat aaatttaata tactagcccg aaggggagca atacataaaa
       61 aatgaaaggc gctccttgaa aacgcccaag gtaatattaa cataaaatgg ctactattgc
      121 attatctaat tttatttaat taaaacaaat atatagcaca aaaaaactag cccgaaggct
      181 agcttatgaa tagatgaaaa tttgaacaca ttgctgtgtc taaaatgatt atagcataat
      241 ttcaaactat tctataataa attaattaac ttcatatgaa actttacaca ctttttttca
      301 aagtgcgcta aaattaccaa aactgcttat tctattacct gccttgtcta cctctcctgt
      361 cgctatataa caacgttgtc cattattagc aatataagta atccatctat agccattaat
      421 gcaatatgcg ccgtcatatt tgatcgttgc gttattgggt aatacacctg taattcttga
      481 attagttgaa tagccatccc tcacgttatt acctttaaca ttggcaactg tgtaataacc
      541 agtctctttt ttatacggta cattgttttt atcgagtgta taacctgctg gcactggtga
      601 attcttttca tttttagctg gtgttttaac attactgata cctgatacac acttccaata
      661 aaaataacca caccatttaa gatgcggtgt agcgactgta atatttctat gttgttgaga
      721 tatatgtatc gagtgaaggg caaaaaggat atcaattgca ggataagtgt taggttacta
      781 ggccacttaa caggctatat agttcactcc tactatatac acttaattat aacataaaaa
      841 gcaccccgta aactattata cgggaatgct aaagtcatat atactacggg ggagtagtat
      901 gaaaaccatg ctctctatca taagaaaaaa acacccagtg acatgcttgg gtgaacaagg
      961 atagatgtaa atagttgatg catgtgtaac acatcatagc aaaaaactag cccgaaagct
     1021 agctataaca tacaatctaa aaagacgtcc tttgaatacg tctagaaaga ttataacata
     1081 aaaaaatagg caagtaccga agtacctgcc taaaaaagga ttatccactt tttcatccta
     1141 actgatttct ccccataagt cacctaatat ctgattaggt ggggaagaac cattcgtgca
     1201 tgaatgagaa tttgatgaaa gataattttc actacacata ttcaatcaag acattgcttt
     1261 ctataataaa caactatttt gttttatttc ttttctataa caacctttgt aattaagttg
     1321 aatccagggt ttttaatatg ctctgataaa tctgggacaa caaaaccttt ttctgttata
     1381 gggaaagact tcgtttcttc ttttttctta ttcttatcat aataagtgac ttcgatcttt
     1441 gcgcttggat ctaattcaac tactctaaac tctttatatg ctgtcgcatc taatgcccat
     1501 tcgacatagt attcaatttt ttcttttgta agtgtagtcc caggtttaat aggaaactcg
     1561 acataatgag gggatagcaa ttcatttcct ttaccatcaa ctccagtcac atttaccatc
     1621 aaatacgggc ctgttggttc aaaataactc gcgtcatcgc cttttttata ttttcctttg
     1681 tcgaatgaac ttgatgcact tacctcatta gtaattgaag aaaatgagaa taataacaat
     1741 aaaacagtta aaaataataa acttc
//
LOCUS       DAXWFK010000270         1760 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.274, whole genome shotgun sequence.
ACCESSION   DAXWFK010000270 DAXWFK010000000
VERSION     DAXWFK010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.274"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..111
                     /locus_tag="ACGEXS_002644"
     CDS             <1..111
                     /locus_tag="ACGEXS_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein"
                     /protein_id="HFF8013592.1"
                     /translation="NNYTIDLSKRLPSTDANRYVKKPQNAKIEVILEKSN"
     gene            219..935
                     /locus_tag="ACGEXS_002645"
     CDS             219..935
                     /locus_tag="ACGEXS_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL12"
                     /protein_id="HFF8013593.1"
                     /translation="MSKNITKNIILTTTLLLLGTVLPQNQKPVFSFYSEAKAYSIGQD
                     ETNINELIKYYTQPHFSFSNKWLYQYDNGNIYVELKRYSWSAHISLWGAESWGNINQL
                     KDRYVDVFGLKDKDTDQLWWSYRETFTGGVTPAAKPSDKTYNLFVQYKDKLQTIIGAH
                     KIYQGNKPVLTLKEIDFRAREALIKNKILYNENRNKGKLKITGGGNNYTIDLSKRLHS
                     DLANVYVKNPNKITVDVLFD"
     gene            complement(1492..1689)
                     /locus_tag="ACGEXS_002646"
     CDS             complement(1492..1689)
                     /locus_tag="ACGEXS_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013594.1"
                     /translation="MTELNNIINSLQSLFESESGYKISKNSGVPYQTVQDLRNGKTKL
                     EDARFRTIIKLYSYYVSLKEH"
     gene            complement(1673..>1760)
                     /locus_tag="ACGEXS_002647"
     CDS             complement(1673..>1760)
                     /locus_tag="ACGEXS_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013595.1"
                     /translation="EIIHTLTVLITTLYIVNSRKGIKNDRVE"
BASE COUNT          612 a          278 c          272 g          598 t
ORIGIN      
        1 aataactaca caatagattt aagtaaaagg ttgccatcaa ctgatgcaaa tagatatgtt
       61 aaaaaacctc aaaatgcaaa aattgaagtt atcctcgaaa aatcaaacta acaataataa
      121 tggagttaat aaaaataatc gcaaatacta tattgacttc gctcacattt aaatttctta
      181 ttcctcgtat catgattcct ctgaaaggag atgttctaat gagtaagaac atcacgaaaa
      241 atataatttt aacgacaaca ttattactat taggtactgt attacctcaa aatcaaaaac
      301 cagtatttag tttttactct gaagctaaag cttatagcat tggtcaagat gaaactaaca
      361 tcaatgaatt aattaaatat tacacacagc ctcatttttc attttcaaat aaatggctat
      421 atcaatatga taatggaaac atttatgttg aacttaagag atattcatgg tcagcacata
      481 tatctttatg gggcgctgaa agttggggaa atattaatca gttaaaagat cgttacgtag
      541 atgtgtttgg actaaaagac aaagatactg atcagttatg gtggtcttat agagagacat
      601 ttacaggtgg cgttacacca gccgcaaaac cttctgataa aacttataat ctttttgtgc
      661 aatacaaaga taaactacaa acgattattg gtgcgcataa aatataccaa ggcaataaac
      721 cagtattaac attgaaagaa atcgatttcc gtgcacgaga agcgttaata aaaaataaaa
      781 tattatataa cgaaaatcgt aataaaggta agcttaagat caccggtggc ggtaataact
      841 acactattga tttaagcaaa agattacatt cagatctagc aaatgtttat gttaaaaatc
      901 ctaataaaat aactgttgac gtcctctttg attagtatat gaaggtgact tatacttcat
      961 gcactttaat tccaaatcag attatttaaa tgataatttt taaagtgtat gatgtatata
     1021 ataggtaaaa ttttctatat atttaaatgg aattgggagt aggaatgtga cagaaatagt
     1081 attttataaa atttatttcg ttgtcactcc ccaacttgca cattattgta agctgacttt
     1141 ccgccagctt ctatgttggg gccccgccaa cttgcattgt ctgtagaaat tgggaatcca
     1201 atttcactat gttggggccc cgccaacttg cacattattg taagctgact tttcgtcagc
     1261 ttctgtgttg gggcccaccc caacttgcat tgtctgtaga atttcttttt gaaattctct
     1321 atgttggggc cccgcctata attgaaaaat gcttgttaca tgggcatttt cattcggtca
     1381 actactacca atataatatt gtagagccta agacattgat ttattatgtc ttaggctcta
     1441 ttccttcatt taatgattaa attattatag caatacttta ttgtcccatg attagtgttc
     1501 ttttaatgag acatagtaac tataaagttt aataatcgtt ctaaatctag catcttctag
     1561 tttggttttc ccatttctta aatcttgtac tgtttgatat ggaactcctg aatttttcga
     1621 aattttatag cctgattcag actcaaacaa agattgaaga gagtttataa tattattcaa
     1681 ctctgtcatt tttaatcccc tttcttgaat taacaatata caatgttgtt attaaaacag
     1741 tcagtgtatg gatgatttca
//
LOCUS       DAXWFK010000271         1757 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.275, whole genome shotgun sequence.
ACCESSION   DAXWFK010000271 DAXWFK010000000
VERSION     DAXWFK010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.275"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            46..>1757
                     /gene="sasG"
                     /locus_tag="ACGEXS_002648"
     CDS             46..>1757
                     /gene="sasG"
                     /locus_tag="ACGEXS_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored surface protein SasG"
                     /protein_id="HFF8013596.1"
                     /translation="MRDKKGPVNKRVDFLSNKLNKYSIRKFTVGTASILIGSLMYLGT
                     QQEAEAAENNIENPTTLKDNVQSKEVKIEEVTNKDTAPQGVEAKSEVTSNKDTIEHEP
                     SVKAEDISKKEDTPKEVADVAEVQPKSSVTHNAETPKVRKARSVDEGSFDITRDSKNV
                     VESTPITIQGKEHFEGYGSVDIQKKPTDLGVSEVTRFNVGNESNGLIGALQLKNKIDF
                     SKDFNFKVRVANNHQSNTTGADGWGFLFSKGNAEEYLTNGGILGDKGLVNSGGFKIDT
                     GYIYTSSMDKTEKQAGQGYRGYGAFVKNDSSGNSQMVGENIDKSKTNFLNYADNSTNT
                     SDGKFHGQRLNDVILTYVASTGKMRAEYAGKTWETSITDLGLSKNQAYNFLITSSQRW
                     GLNQGINANGWMRTDLKGSEFTFTPEAPKTITELEKKVEEIPFKKERKFNPDLAPGTE
                     KVTREGQKGEKTITTPTLKNPLTGEIISKGEPKEEITKDPINELTEYGPETIAPGHRD
                     EFDPKLPTGEKEEVPGKPGIKNPETGDVVRPPVDSVTKYGPVKGDSIVEKEEIPFEKE
                     RKFNP"
BASE COUNT          725 a          244 c          363 g          425 t
ORIGIN      
        1 attcactgta agtaaagtgg aaaatatgga aaaaggagta tgcaaatgag agataagaaa
       61 ggaccggtaa ataaaagagt agattttcta tcaaataaat tgaataaata ttcaataaga
      121 aaatttacag ttggaacagc atctatttta attggctcac taatgtattt gggaactcaa
      181 caagaggcag aagcagctga aaacaatatt gagaatccaa ctacattaaa agataatgtc
      241 caatcaaaag aagtgaagat tgaagaagta acaaacaaag acactgcacc acagggtgta
      301 gaagctaaat ctgaagtaac ttcaaacaaa gacacaatcg aacatgaacc atcagtaaaa
      361 gctgaagata tatcaaaaaa ggaggataca ccaaaagaag tagctgatgt tgctgaagtt
      421 cagccgaaat cgtcagtcac tcataacgca gagacaccta aggttagaaa agctcgttct
      481 gttgatgaag gctcttttga tattacaaga gattctaaaa atgtagttga atctacccca
      541 attacaattc aaggtaaaga acattttgaa ggttacggaa gtgttgatat acaaaaaaaa
      601 ccaacagatt taggggtatc agaggtaacc aggtttaatg ttggtaatga aagtaatggt
      661 ttgataggag ctttacaatt aaaaaataaa atagatttta gtaaggattt caattttaaa
      721 gttagagtgg caaataacca tcaatcaaat accacaggtg ctgatggttg ggggttctta
      781 tttagtaaag gaaatgcaga agaatattta actaatggtg gaatccttgg ggataaaggt
      841 ctggtaaatt caggcggatt taaaattgat actggataca tttatacaag ttccatggac
      901 aaaactgaaa agcaagctgg acaaggttat agaggatacg gagcttttgt gaaaaatgac
      961 agttctggta attcacaaat ggttggagaa aatattgata aatcaaaaac taatttttta
     1021 aactatgcgg acaattcaac taatacatca gatggaaagt ttcatgggca acgtttaaat
     1081 gatgtcatct taacttatgt tgcttcaact ggtaaaatga gagcagaata tgctggtaaa
     1141 acttgggaga cttcaataac agatttaggt ttatctaaaa atcaggcata taatttctta
     1201 attacatcta gtcaaagatg gggccttaat caagggataa atgcaaatgg ctggatgaga
     1261 actgacttga aaggttcaga gtttactttt acaccagaag cgccaaaaac aataacagaa
     1321 ttagaaaaaa aagttgaaga gattccattc aagaaagaac gtaaatttaa tccggattta
     1381 gcaccaggga cagaaaaagt aacaagagaa ggacaaaaag gtgagaagac aataacgaca
     1441 ccaacactaa aaaatccatt aactggagaa attattagta aaggtgaacc aaaagaagag
     1501 attacaaaag atccgattaa tgaattaaca gaatacggac ctgaaacaat agcgccaggt
     1561 catcgagacg aatttgatcc gaagttacca acaggagaga aagaggaagt tccaggtaaa
     1621 ccaggaatta agaatccaga aacaggagac gtagttagac cgccggtcga tagcgtaaca
     1681 aaatatggac ctgtaaaagg agactcgatt gtagaaaaag aagagattcc attcgagaaa
     1741 gaacgtaaat ttaatcc
//
LOCUS       DAXWFK010000272         1753 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.276, whole genome shotgun sequence.
ACCESSION   DAXWFK010000272 DAXWFK010000000
VERSION     DAXWFK010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.276"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..77
                     /locus_tag="ACGEXS_002649"
     CDS             <1..77
                     /locus_tag="ACGEXS_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013597.1"
                     /translation="SLLIFIYLIIIFYFTRREQHENKN"
     gene            61..576
                     /locus_tag="ACGEXS_002650"
     CDS             61..576
                     /locus_tag="ACGEXS_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HFF8013598.1"
                     /translation="MKIKTEFKGNNIPYEYAAGADVSDSINGNPIKSFPFEVIELPEG
                     TKYLAWSLIDYDAIPVCGFAWIHWSVANVSVSGNSISIKADLSRTKGDYVQGKNSFTS
                     GLLAEDFSEIENHYVGPTPPDQDHQYELTVYALDHSLNLKNGFYLNEFLKEVNQHKID
                     QTSINLIGRKI"
     gene            887..1321
                     /locus_tag="ACGEXS_002651"
     CDS             887..1321
                     /locus_tag="ACGEXS_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HFF8013599.1"
                     /translation="MKLKSFITVTLALGMIATTGATVAGNEVSAAEKDKLPATQKAKE
                     MQNVPYTIAVDGIMAFNQSYLNLPKDSQLSYLDLGNKVKALLYDERGVTPEKIRNAKS
                     AVYTITWKDGSKKEVDLKKDSYTANLFDSNSIKQIDINVKTK"
     gene            complement(1578..>1753)
                     /locus_tag="ACGEXS_002652"
     CDS             complement(1578..>1753)
                     /locus_tag="ACGEXS_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFF8013600.1"
                     /translation="VTFFWALLLMQMVNFVLNSLVGGDSLNVVNPIIMAVLFTIFTAI
                     FAAVIKPPKDSSQ"
BASE COUNT          678 a          226 c          282 g          567 t
ORIGIN      
        1 tttcgttatt aatttttata tatttaataa taatatttta ttttacaagg agagagcaac
       61 atgaaaataa aaactgaatt taaagggaac aatataccat atgaatacgc agcaggtgca
      121 gatgtgagtg attctattaa cgggaatcca attaagtcat ttccatttga agtaattgaa
      181 ttaccggaag ggactaaata tcttgcttgg tctttaattg actatgatgc aattcctgta
      241 tgtggctttg cttggattca ttggagtgta gctaatgtaa gtgttagtgg caattcaatt
      301 tctataaaag cagatttatc aagaacaaag ggcgactatg tacaaggtaa aaatagcttt
      361 actagtgggt tgttggctga agatttttca gaaatagaaa atcactatgt aggacctaca
      421 ccacctgatc aagatcatca atatgaatta acagtttatg cgttagatca ttctttaaat
      481 ttgaagaatg ggttctactt gaatgaattt ttaaaagaag taaatcaaca taaaattgat
      541 caaacaagta ttaaccttat aggaagaaaa atttaatact aaatatctca tcaatataaa
      601 attgttcaat taaaagtaca aagaaacaaa ggttttaatt tatatattag gtacggcgtt
      661 cgctataatg caaagaagta attaaattta agaaatgtaa acttagttat tgtaatgtga
      721 atttatttga aaaaatagaa agtattaaca attatagctt ttacattaat taaaatttat
      781 ttttaaaaac aagtaaacaa tttacatact tataattttt gaaacttttc aatttgtgtt
      841 atattgattt tgtaagatac tttaactcac aaaggagaga gattatatga aattaaaatc
      901 atttataact gtaactttgg cactgggcat gatcgcaacg actggcgcta ctgtggcagg
      961 taatgaggta tctgcagcag aaaaggacaa actaccggca actcaaaaag ctaaagaaat
     1021 gcaaaatgtt ccatatacaa ttgcagtaga tggcattatg gctttcaatc aatcttactt
     1081 aaatttacca aaagatagcc aattatcata tttagattta ggaaataaag ttaaagcttt
     1141 gttatatgat gaacgcggtg taacacctga gaagattcga aatgcaaaat ctgccgttta
     1201 cacgattact tggaaagatg gtagtaaaaa agaagtggat cttaagaaag atagctacac
     1261 agcaaacttg tttgattcaa attcaattaa acaaattgat attaatgtaa aaactaaata
     1321 aacacttaaa attatcaaat tcaacatgaa gcatttaatt ttacagtgat gattataaaa
     1381 taattgcctt gatacaaaga ttactcgtaa atgacatctt tgtattaagg ctttttctaa
     1441 atttaaaagt gatgggttag aggtcattga gctttaaaat attcaaaata caaaacatta
     1501 atggccaaaa ataaaagccg cctttatctg ggcagcttca ataataagaa agacatattt
     1561 cattttatac taaatagtta ttgtgatgaa tctttcggcg gtttaattac tgcagcaaaa
     1621 attgctgtga aaatcgtgaa caatactgcc atgataattg gattcactac atttaagctg
     1681 tctccaccta ctaggctatt aagtacaaag ttaaccattt gcattaataa taatgcccaa
     1741 aagaatgtta cga
//
LOCUS       DAXWFK010000273         1743 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.277, whole genome shotgun sequence.
ACCESSION   DAXWFK010000273 DAXWFK010000000
VERSION     DAXWFK010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.277"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            120..353
                     /locus_tag="ACGEXS_002653"
     CDS             120..353
                     /locus_tag="ACGEXS_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013601.1"
                     /translation="MVFNDWEINVVYSGEHEVVTNEKSLFVIDEFYDVAIAIYYLDGK
                     LKVAHVNYGSDFTIDVANKVFELNIQNPNVDEH"
     gene            975..1160
                     /locus_tag="ACGEXS_002654"
     CDS             975..1160
                     /locus_tag="ACGEXS_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013602.1"
                     /translation="MNKHINKKAAKLLEDYKKVQQRKSKETDRGCLKSVIMLIVLFFL
                     IIFGIVACSTNVNFFFQ"
     gene            complement(1367..1552)
                     /locus_tag="ACGEXS_002655"
     CDS             complement(1367..1552)
                     /locus_tag="ACGEXS_002655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013603.1"
                     /translation="MGPQQREIGSPIPTDNASWRGPNIEKLDHQFQQTMQVGVGPNTE
                     AGEKSAYKNVQVGGAQT"
BASE COUNT          624 a          236 c          260 g          623 t
ORIGIN      
        1 acatataatt gaaaaagatt tctagaaata caattaatgt tcatctagat attgtacgaa
       61 gttgtatgaa taagttaaac ttaagttgaa tcaatttaaa tgttcaagga ggttaacaaa
      121 tggtttttaa tgattgggaa attaacgttg tatactcagg agagcatgag gttgtaacaa
      181 acgaaaagag tttatttgtc attgacgaat tttacgatgt tgcaatcgca atctattatt
      241 tagatggaaa attaaaagta gctcatgtca actatggttc tgattttaca attgacgtag
      301 ctaataaagt ttttgaattg aatattcaaa atccaaatgt tgatgagcat taaatattaa
      361 aaagcattta gaatcataaa gagcatacta tatatagcct tttacatgta aagcaaatga
      421 aaattagata atctaattat ttcaacatta aacaatatat cattataaag aagtttttaa
      481 aatagaagta taaggtggtg aacaaaaatt aacattgtgt ataacattat agtatatata
      541 gcatgcatga ttaatttcgg attaattata gtcccattat taacaacaaa tgatgcggat
      601 tttgtatgga cttcttcatc attaatatat atgattcttc ttacgtcgat tatcctaata
      661 gtttatatta aaactaaaga tagcttacag ttaacgatat ttatacttaa tatagcctta
      721 attattctat actgtgcacc attactattc atttatgtat tctagtgctt cattaatatt
      781 ttaatgcatc agtttaagta aattccattt ggaaaataaa aagcaaatat catcatcaaa
      841 ttaacatatt taacgttata ctttcataaa atttgaaaca attgaacatc cttactttag
      901 ctttatatct ttatttcaag tcatgttttc aactatttta atgaattaaa catatattat
      961 aaggagtcaa catcatgaat aaacatataa ataaaaaagc cgcaaaacta cttgaagatt
     1021 ataaaaaggt tcaacaaaga aaatcgaaag aaactgatag aggttgtttg aaaagtgtca
     1081 tcatgcttat cgtcttattt ttcttaatta tttttggtat tgtggcatgt tctacaaacg
     1141 taaacttttt cttccagtaa aagagtgaga tagaaatgat aaagaaccac taatgattta
     1201 ttatgtagtg gttctttatc attagctgta gctaatgtgt acttaaaaaa tgaatacatg
     1261 agtaaagctc atgcataaga aatactaatt gctaaagaaa aagtatttct ttatcgatgt
     1321 cctaccccaa cttgcattgt ctgtagaaat tggggttcca atttctctat gtttgggccc
     1381 cgccaacttg cacatttttg taagctgact tttcgccagc ttctgtgttg ggccccaccc
     1441 caacttgcat tgtctgttga aattggtgat ccaatttctc tatgttgggg ccccgccaac
     1501 ttgcattgtc tgttggaatt ggtgatccta tttctctctg ttgtggcccc acgagtattg
     1561 aaaaaacttg ttataagctt attttcattt agtcaactac taccaatata gcactagaac
     1621 tggaaatgat aatttaatat tgtacacttt tgattgttta aacatgtaca tattttaaaa
     1681 aataggagag caaagtaaat aattgatata gttattttga gagtaatcct aggaactatt
     1741 gta
//
LOCUS       DAXWFK010000274         1735 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.278, whole genome shotgun sequence.
ACCESSION   DAXWFK010000274 DAXWFK010000000
VERSION     DAXWFK010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.278"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            117..656
                     /locus_tag="ACGEXS_002656"
     CDS             117..656
                     /locus_tag="ACGEXS_002656"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFF8013604.1"
                     /translation="MTKNEIRKYILHKMKNFNKAEKRKADTWLRNQFFATEEYKEANA
                     IALVLSFNHEVDTFSIIEQALMDHKRIFVPKMDYLNHQMTFKEIFNLKDIDVDNKGIY
                     YPTSKGETTNNLDLIVVPGVGFQDDGYRIGYGGGYYDRFLANYQTKTISLLYDFQITS
                     FEPESFDQPVDKLIIYQAA"
     gene            668..>1735
                     /locus_tag="ACGEXS_002657"
     CDS             668..>1735
                     /locus_tag="ACGEXS_002657"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001652331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFF8013605.1"
                     /translation="MNIDKQFWKTIYYWIRYLNFDIVSREKDDQEIWLAHKRKKQVVI
                     FKQHIKSTQEIRFDKAKVLEHKDEIANFISFEPQSFEFYYFTESEFSEEQLNEVSPIR
                     IKFNVIRHTKDLIKHMPNIFLARLISEDNDKKTYMFYKRKVLTDNFLDKYMQKFSPAT
                     YTIIFVNVLIWLCMILYLNNFSDVKLLDVGGLVHFNVVHGEWYRIVTSMFLHFSFEHI
                     LMNMLSLFIFGKIVEAIIGSWRMLTVYFIAGLFGNFVSLSFNTTTISVGASGAIFGLI
                     GSIFAMMYVSKTFNKKMLGQLLIALVILVGVSLFMSNINIVAHIGGFIGGLLITLIGY
                     YYKVNRNIFWILLIGMLVIFIA"
BASE COUNT          633 a          197 c          295 g          610 t
ORIGIN      
        1 aaaagatttt caattgaaat aataaatttt agaattgtcg cataattcat catgacaaca
       61 taatgacatg tattgttatt aacgatataa tagaattgaa taaagtgggt gataatgtga
      121 ctaaaaatga gattagaaaa tacattttac ataaaatgaa gaattttaat aaagctgaaa
      181 agcgaaaagc agacacatgg ttaagaaatc aattttttgc aactgaagaa tacaaagaag
      241 caaacgcaat tgcgctagtt ctttctttta atcatgaagt agatactttt tctattattg
      301 aacaagcctt aatggatcat aaacgtattt ttgtaccgaa aatggattat ttaaatcatc
      361 aaatgacttt taaagagata tttaatctca aagatattga tgtcgataat aaggggattt
      421 actatccaac ttcaaaaggt gaaacaacga ataacctaga tttaattgtt gttcctggtg
      481 ttggatttca agacgatgga tatagaattg ggtatggtgg tggctattac gacaggtttt
      541 tagctaatta tcagacaaag acaataagct tattatacga ttttcaaata acatcatttg
      601 aaccggaatc attcgatcaa ccagtcgata aattgattat atatcaagca gcatagtgga
      661 ggactaaatg aacatagaca aacaattttg gaaaacaata tattattgga ttaggtactt
      721 gaattttgat attgtcagta gggaaaaaga tgaccaagaa atttggttag cccataaaag
      781 aaaaaaacaa gtagtcattt ttaaacaaca tatcaagtct acccaagaaa tacgttttga
      841 caaagcgaaa gtgcttgaac acaaagatga aatagcaaat tttatttctt tcgaaccaca
      901 aagttttgaa ttttattatt ttacagaatc ggaattttca gaagaacaat taaatgaagt
      961 ttcgccaatt agaattaaat tcaatgttat aagacacaca aaagatttga taaagcatat
     1021 gccgaatata tttttggcta gacttatttc agaagataat gataaaaaga catatatgtt
     1081 ctataaacgc aaagtattaa ccgataactt tttagataaa tatatgcaga aattttcacc
     1141 ggcaacatac acaataatat ttgtaaatgt cttaatatgg ttatgtatga ttttatattt
     1201 aaataatttt tcggatgtaa aattattaga tgttggcggg ttagtgcatt ttaatgtcgt
     1261 acatggtgaa tggtatcgaa ttgttacatc gatgttttta cattttagtt ttgaacatat
     1321 acttatgaat atgctttcat tatttatttt tggtaaaata gtcgaagcaa ttattggttc
     1381 atggcggatg ttaactgtat actttattgc agggttgttt ggaaactttg tatcactatc
     1441 atttaatacg actacaattt cagttggggc tagtggtgct atatttggtc tgattggatc
     1501 aatttttgcg atgatgtatg tttcaaaaac atttaacaaa aaaatgttag gacagttatt
     1561 aattgcatta gtgatattag ttggtgtttc tctgtttatg tcaaatataa atattgtggc
     1621 gcatattgga ggattcattg gtggtttatt aataacttta attggctatt actataaagt
     1681 gaatcgtaat attttttgga ttttactaat tggtatgctt gttatattta ttgca
//
LOCUS       DAXWFK010000275         1711 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.279, whole genome shotgun sequence.
ACCESSION   DAXWFK010000275 DAXWFK010000000
VERSION     DAXWFK010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.279"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..324)
                     /locus_tag="ACGEXS_002658"
     CDS             complement(<1..324)
                     /locus_tag="ACGEXS_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HFF8013606.1"
                     /translation="MFKKAKLILIATLLLSGCSAMNNESKKDTNTETNTETKSVPEEM
                     EASKYVGQGFQPPAEKDAIEFVKKHRKEFEKVGEQFFKDNFGLKVKATNVVGKDDGVE
                     VYVHCE"
     gene            complement(381..1298)
                     /locus_tag="ACGEXS_002659"
     CDS             complement(381..1298)
                     /locus_tag="ACGEXS_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HFF8013607.1"
                     /translation="MFKRTKLILIATLLLSGCSTTNNESNKETKSVPEEMEASKYVGQ
                     GFQPPAEKDVVEFAKKHKDKIAKRGEQFFMDNFGLKVKATNVVGSGKGVEVFVHCDDH
                     DIVFNASIPFDKSIIESDSSLRSEDKGDDMSTLVGTVLSGFEYRTQKEKYDNLYKFFK
                     DNEEKYQYTGFTKEAINKTQNVGYKNEYFYITYSSRSLKEYRKYYEPLIHKNDKEFKE
                     GMEQARKEVNYAANTDTVTTLFSTKENFTKDNTVDDVIELSDKLYNFKNKPEKSTITI
                     QIGKPTINTKKAFYDDNDPIEYGVYRKDE"
     gene            complement(1389..>1711)
                     /locus_tag="ACGEXS_002660"
     CDS             complement(1389..>1711)
                     /locus_tag="ACGEXS_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013608.1"
                     /translation="LGKWEPFLNDLKAVSPILDNHLDDIVKNMKPLIVQMIFEPSHYD
                     DMFILNTQAHARLDQMAQQFEVVCNGLNENEGQAIQTMDQSASLIRSNLIQVKEQLEK
                     LAVY"
BASE COUNT          482 a          304 c          223 g          702 t
ORIGIN      
        1 ttcgcaatgc acataaactt ctacaccatc atctttacct acaacatttg tagctttaac
       61 ttttagtcca aagttatctt taaagaattg ttcacctact ttttcaaatt ctttacgatg
      121 cttcttcaca aattcaatcg catctttttc tgcaggtggt tgaaagcctt gtcctacata
      181 ttttgaagct tccatttctt ctggtactga ttttgtttct gtatttgttt ctgtatttgt
      241 gtcttttttt gattcattat tcatcgctga acatcctgat aacagtagcg ttgctattaa
      301 gattaatttc gcctttttaa acatagctca tcacccattt atgtgtttat atagaatatt
      361 aaaaagcatt ttaaaaaaat ttattcatct ttacgataca ctccatattc tattggatca
      421 ttatcatcat aaaaggcttt tttagtatta atagtaggct ttcctatttg aattgtaatt
      481 gtagattttt ctggcttatt tttaaaatta tatagtttat cactcagttc aattacatca
      541 tcaactgtat tgtctttagt aaaattttcc tttgtactaa acaatgttgt tactgtatct
      601 gtattagcag cgtaattcac ttcttttctg gcttgttcca ttccttcttt aaattcttta
      661 tcatttttat gaatcaatgg ttcgtaatac ttacgatatt cttttaagct tcttgacgag
      721 taagtgatat aaaaatattc atttttatat ccgacatttt gcgtcttgtt tattgcttct
      781 tttgtaaagc ctgtatattg gtatttctct tcattatctt tgaaaaattt atataaattg
      841 tcatactttt ctttttgtgt tcgatattca aagccactca acactgtacc aactaaagta
      901 ctcatatcat cgcctttgtc ctcacttctt aatgagctat cactctcaat tattgattta
      961 tcaaatggaa tactcgcatt aaatacgata tcgtggtcat cacaatgcac gaatacttct
     1021 acgccttttc cactacctac aacattggta gctttaactt ttagaccgaa attatccata
     1081 aaaaattgtt cgcctcgttt ggcaatttta tctttatgct ttttcgcaaa ttcaaccaca
     1141 tctttttctg caggtggttg gaagccttga cctacatatt ttgaagcttc catttcttct
     1201 ggcactgatt ttgtttcttt attagattcg ttattggtag ttgaacatcc tgatagcagt
     1261 aatgttgcta ttaagattag tttagttctt ttaaacatag ctcatcaccc atttatgtgt
     1321 ttgtaaaact tttatgtttg aaaaagctac ttattctcaa tgaaaacaag tagcatttaa
     1381 taaattaatt agtatacagc tagtttttct aattgttctt taacttgaat taagtttgac
     1441 cgtattagag aggcagattg atccatcgtt tgaattgctt gtccttcatt ttcgttcaag
     1501 ccattacaaa caacttcaaa ctgttgtgcc atttgatcaa gacgcgcatg agcttgtgtg
     1561 tttaaaataa acatatcgtc ataatgtgat ggttcaaata tcatttgtac aatcaaaggc
     1621 ttcatgttct taacaatatc atctaaatgg ttatctaaaa ttggtgacac tgcttttaaa
     1681 tcattaagaa aaggctccca tttgcctaaa g
//
LOCUS       DAXWFK010000276         1681 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.280, whole genome shotgun sequence.
ACCESSION   DAXWFK010000276 DAXWFK010000000
VERSION     DAXWFK010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.280"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..390
                     /locus_tag="ACGEXS_002661"
     CDS             <1..390
                     /locus_tag="ACGEXS_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013609.1"
                     /translation="FIGYISLAISAVIIYGIIQFITIDFGSFFLMGIILILISSIFQL
                     FIVRLLFRKKNVDLTEVVVLEHLIQWFLVYFAIYQAVNEKMDINDINIDNFQSVFFDV
                     SNLNLVILPTLIISWVTIFNYRMRSYK"
     gene            649..1146
                     /gene="efb"
                     /locus_tag="ACGEXS_002662"
     CDS             649..1146
                     /gene="efb"
                     /locus_tag="ACGEXS_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement convertase inhibitor Efb"
                     /protein_id="HFF8013610.1"
                     /translation="MKNKLIAKSLLTLAAIGITTTTIASTADASEGYGPREKKPVSIN
                     HNIVEYNDGTFKYQSRPKFNSTPKYIKFKHDYNILEFNDGTFEYGARPQFNKPAAKTD
                     ATIKKEQKLIQAQNLVREFEKTHTVSAHRKAQKAVNLVSFEYKVKKMVLQERIDNVLK
                     QGLVK"
     gene            1300..1650
                     /gene="scb"
                     /locus_tag="ACGEXS_002663"
     CDS             1300..1650
                     /gene="scb"
                     /locus_tag="ACGEXS_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN-B"
                     /protein_id="HFF8013611.1"
                     /translation="MKFKKYILTGTLALLLSSTGIATIEGNKADASSLDKYLTESQFH
                     DKRIAEELRTLLNKSNVYALAAGSLNPYYKRTIMMNEYRAKAALKKNDFVSMADAKVA
                     LEKIYKEIDEIINR"
BASE COUNT          660 a          199 c          272 g          550 t
ORIGIN      
        1 tttataggat atatttcact ggcaatatct gccgtaatta tttatggtat tattcaattt
       61 atcacaattg attttggtag ttttttctta atgggtatta tattgatctt gatttcaagt
      121 atattccaat tatttatagt tagattactt tttagaaaaa agaatgtcga tttgacagag
      181 gttgtcgttt tagagcattt aattcaatgg ttcttagttt actttgcgat ctatcaagca
      241 gtaaatgaaa aaatggacat taatgatatt aatatcgaca atttccaatc tgtctttttt
      301 gacgtgtcta atttgaattt agtaattcta ccaacgttaa tcattagctg ggtcacaata
      361 tttaactata gaatgagaag ttacaaataa aatctatgag attatacctt cagacaccaa
      421 cattcaaatg gtgtcttttt tgttgtgtgg ttttattttt gaaattcgaa aaagtagagg
      481 catgaatttt ttgactagtg tataagtgct gatgagtcac aagatagata gctatatttt
      541 gtctatatta taaagtgttt atagttaatt aataattagt taatttcaaa agttgtataa
      601 ataggataac ttaataaatg taagataata atttggagga taattaacat gaaaaataaa
      661 ttgatagcaa aatctttatt aacattagcg gcaataggta ttactacaac tacaattgcg
      721 tcaacagcag atgcgagcga aggatacggt ccaagagaaa agaaaccagt gagtattaat
      781 cacaatatcg tagagtacaa tgatggtact tttaaatatc aatctagacc aaaatttaac
      841 tcaacaccta aatatattaa attcaaacat gactataata ttttagaatt taacgatggt
      901 acattcgaat atggtgcacg tccacaattt aataaaccag cagcgaaaac tgatgcaact
      961 attaaaaaag aacaaaaatt gattcaagct caaaatcttg tgagagaatt tgaaaaaaca
     1021 catactgtca gtgcacacag aaaagcacaa aaggcagtca acttagtttc gtttgaatac
     1081 aaagtgaaga aaatggtctt acaagagcga attgataatg tattaaaaca aggattagtt
     1141 aaataaaact tcaatcgttg ctgttatctg gaaataatta attaaatgtt atgttaattt
     1201 ttgttaatga aaaaagtaat ctatttaatg acaggttaat gtaattgtcc tgaaattgac
     1261 tatatactca gtaagtatca attttaagga gagcttataa tgaaatttaa aaaatatata
     1321 ttaacaggaa cattagcatt acttttatca tcaactggga tagcaactat agaagggaat
     1381 aaagcagatg caagtagtct ggacaaatat ttaactgaaa gtcagtttca tgataaacgc
     1441 atagcagaag aattaagaac tttacttaac aaatcgaatg tatatgcatt agctgcagga
     1501 agcttaaatc catattataa acgtacgatt atgatgaatg aatatagagc taaagcggca
     1561 cttaagaaaa atgatttcgt atcaatggct gatgctaaag ttgcattaga aaaaatatac
     1621 aaagaaattg atgaaattat aaatagataa taaataaaac aggttgagac aaaaaatggt
     1681 c
//
LOCUS       DAXWFK010000277         1680 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.281, whole genome shotgun sequence.
ACCESSION   DAXWFK010000277 DAXWFK010000000
VERSION     DAXWFK010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.281"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(101..1669)
                     /locus_tag="ACGEXS_002664"
     CDS             complement(101..1669)
                     /locus_tag="ACGEXS_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFF8013612.1"
                     /translation="MIEKLVTFLNEVVWSKPLVYGLLITGVLFTLRMRFFQVRHFKEM
                     IRLMFQGEKSPNGISSFQAIAMSLAGRVGTGNIVGVSTAIFIGGPGAVFWMWITAFLG
                     ASSAFIESTLGQIFKRVENNEYRGGPAYYIEYGIGGKFGKIYGIIFAIVTIISVGLLL
                     PGVQSNAIASSMHNAIHVPQWLMGGIVVVILGLIIFGGVRSIANVATAVVPFMAIIYI
                     LMAVIIICINIQEVPALFALIFKSAFGLQSAFGGIVGAMIEIGVKRGLYSNEAGQGTG
                     PHAAAAAEVSHPSKQGLVQAFSVYIDTLFVCTATALIILISGTYNVTDGTVNANGTPH
                     LIKDGGIYVENATGKDYSGTAMYAQAGIDKAFHGSGYQFDPTFSGVGSYFIAFALFFF
                     AFTTILSYYYITETNVAYLTRNQNNQVSSIFINIARVIILFATFYGAVKTADVAWAFG
                     DLGVGLMAWLNIIAIWILHKPAVNALKDYEIQKKRLGNGYNAVYQPDPNKLPNAVFWL
                     KTYPERLKQARAKK"
BASE COUNT          602 a          355 c          235 g          488 t
ORIGIN      
        1 gacactatat ttttaaaatt caaatttctg agcacctaaa attctcaata cttttctttt
       61 ttatcaaatg atcactacat atactataaa caaaagtaga ttactttttg gcacgtgctt
      121 gttttaaacg ttctggatac gtcttcaacc aaaagacagc attaggtaat ttattcggat
      181 caggttgata aactgcatta taaccgttgc ctaaacgttt cttttgaatt tcataatctt
      241 ttaaagcatt tacggcaggc ttatgtaaaa tccaaatcgc aatgatattt aaccaagcca
      301 ttagacctac acctaaatca ccgaatgccc atgctacatc agctgtttta actgcaccgt
      361 aaaatgtagc gaacaaaata atcacacgag caatattaat aaatatcgat gaaacttgat
      421 tattttgatt acgcgttaaa taagcaacat ttgtttctgt aatgtagtag tacgacaaaa
      481 ttgtagtaaa tgcaaagaag aataaagcaa atgcaataaa gtacgaacct acgccagaga
      541 aagtaggatc aaattgataa ccactgccat ggaacgcttt atcaatgccg gcttgtgcat
      601 acatcgcagt acctgaataa tctttacctg ttgcattttc aacataaata ccgccatctt
      661 taattaaatg cggtgtgcca ttcgcattaa ccgtaccatc agtcacatta tatgtaccag
      721 aaataagtat aatcagagca gttgcagtac atacaaataa tgtatcaata taaactgaaa
      781 atgcttgtac tagaccttgt ttacttggat gtgatacttc tgctgccgct gctgcgtgtg
      841 gacctgtacc ttgaccagcc tcatttgaat ataatccacg tttaacacca atctctatca
      901 ttgcgccaac gataccacca aaagcagatt gtaatccaaa tgctgatttg aaaattaatg
      961 caaataacgc tggcacttct tgtatattga tacaaatgat aatgacagcc atcagtatgt
     1021 aaataattgc cataaatggt acaacggctg ttgcaacatt ggcaatacta cgtacaccac
     1081 caaaaataat taatcccaaa ataactacaa caataccacc cattaaccat tgtggaacat
     1141 gaatcgcatt atgcatagaa cttgctatag cgttagattg cacaccagga agcaatagac
     1201 ctactgagat aatcgtaaca atagcaaaga taattccgta aattttacca aatttaccac
     1261 caataccata ttcaatataa tacgctggtc caccacggta ttcattattt tcaactctct
     1321 tgaatatttg accaagtgta gattcaataa aagcactact tgcacctaaa aacgcagtaa
     1381 tccacatcca aaatactgca ccaggtcctc ctataaatat tgcagtagat acaccgacaa
     1441 tattacctgt accaaccctg cctgctaaag acatggctat cgcttgaaaa cttgaaatac
     1501 cattaggaga cttctctcct tgaaacatta atcgaatcat ttctttaaaa tgtctaactt
     1561 gaaaaaatcg catacgcaat gtaaatagca caccagtaat tagcaaacca taaactaatg
     1621 gcttactcca aacaacctca tttaaaaagg ttactaattt ttcaatcata agattccccc
//
LOCUS       DAXWFK010000278         1677 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.282, whole genome shotgun sequence.
ACCESSION   DAXWFK010000278 DAXWFK010000000
VERSION     DAXWFK010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.282"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..173)
                     /locus_tag="ACGEXS_002665"
     CDS             complement(<1..173)
                     /locus_tag="ACGEXS_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFF8013613.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRGRGVNVHH
                     STVYRWVQEYAPI"
     gene            217..363
                     /locus_tag="ACGEXS_002666"
     CDS             217..363
                     /locus_tag="ACGEXS_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001807584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication domain protein"
                     /protein_id="HFF8013614.1"
                     /translation="MQQNQKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNY
                     FIKE"
     gene            523..1293
                     /gene="aadD1"
                     /locus_tag="ACGEXS_002667"
     CDS             523..1293
                     /gene="aadD1"
                     /locus_tag="ACGEXS_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-nucleotidyltransferase
                     ANT(4')-Ia"
                     /protein_id="HFF8013615.1"
                     /translation="MRIVNGPIIMTREERMKIVHEIKERILDKYGDDVKAIGVYGSLG
                     RQTDGPYSDIEMMCVMSTEEAEFSHEWTTGEWKVEVNFDSEEILLDYASQVESDWPLT
                     HGQFFSILPIYDSGGYLEKVYQTAKSVEAQTFHDAICALIVEELFEYAGKWRNIRVQG
                     PTTFLPSLTVQVAMAGAMLIGLHHRICYTTSVSVLTEAVKQSDLPSGYDHLCQFVMSG
                     QLSDSEKLLESLENFWNGIQEWTERHGYIVDVSKRIPF"
     gene            complement(1354..>1677)
                     /locus_tag="ACGEXS_002668"
     CDS             complement(1354..>1677)
                     /locus_tag="ACGEXS_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS257 family transposase"
                     /protein_id="HFF8013616.1"
                     /translation="KRLIKQFGKPQKVITDQAPSTKVAMVKVIKAFKLKPDCHCTSKY
                     LNNLIEQDHRHIKVRKTRYQSINTAKNTLKGIECIYALYKKNRRSLQIYGFSPCHEIS
                     IMLAS"
BASE COUNT          526 a          268 c          362 g          521 t
ORIGIN      
        1 aaaattgggg catattcttg aacccaacgg tagaccgttg aatgatgaac gtttacacca
       61 cgtcccctta atatttcaga tatatcacga taactcaatg catatcttag atagtagcca
      121 acggctacag tgataacatc cttgttaaat tgtttatatc tgaaatagtt catacagaag
      181 actccttttt gttaaaatta tactataaat tcaactttgc aacagaacca aaaattaaac
      241 cttgatgaca cagaagaagg cgatttgatt catacagatg atgacgaaaa agccgatgaa
      301 gatggatttt ctattattgc aatgtggaat tgggaacgga aaaattattt tattaaagag
      361 tagttcaaca aacgggccag tttgttgaag attagatgct ataattgtta ttaaaaggat
      421 tgaaggatgc ttaggaagac gagttattaa tagctgaata agaacggtgc tctccaaata
      481 ttcttattta gaaaagcaaa tctaaaatta tctgaaaagg gaatgagaat agtgaatgga
      541 ccaataataa tgactagaga agaaagaatg aagattgttc atgaaattaa ggaacgaata
      601 ttggataaat atggggatga tgttaaggct attggtgttt atggctctct tggtcgtcag
      661 actgatgggc cctattcgga tattgagatg atgtgtgtca tgtcaacaga ggaagcagag
      721 ttcagccatg aatggacaac cggtgagtgg aaggtggaag tgaattttga tagcgaagag
      781 attctactag attatgcatc tcaggtggaa tcagattggc cgcttacaca tggtcaattt
      841 ttctctattt tgccgattta tgattcaggt ggatacttag agaaagtgta tcaaactgct
      901 aaatcggtag aagcccaaac gttccacgat gcgatttgtg cccttatcgt agaagagctg
      961 tttgaatatg caggcaaatg gcgtaatatt cgtgtgcaag gaccgacaac atttctacca
     1021 tccttgactg tacaggtagc aatggcaggt gccatgttga ttggtctgca tcatcgcatc
     1081 tgttatacga cgagcgtttc ggtcttaact gaagcagtta agcaatcaga tcttccttca
     1141 ggttatgacc atctgtgcca gttcgtaatg tctggtcaac tttccgactc tgagaaactt
     1201 ctggaatcgc tagagaattt ctggaatggg attcaggagt ggacagaacg acacggatat
     1261 atagtggatg tgtcaaaacg cataccattt tgaaggttct gttgcaaagt aaaaaaatat
     1321 agctaaccac taatttatca tgtcagtgtt cgcttaactt gctagcatga tgctaatttc
     1381 gtggcatggc gaaaatccgt agatctgaag agacctgcgg ttctttttat atagagcgta
     1441 aatacactca atacctttta aagtattctt tgctgtattg atactttgat accttgtctt
     1501 tcttacttta atatgacggt gatcttgctc aatgaggtta ttcagatatt tcgatgtaca
     1561 atggcagtca ggtttaagtt taaaagcttt aattacttta accattgcta ccttcgttga
     1621 aggtgcctga tctgtaatta ccttttgagg tttaccaaat tgtttaatga gacgttt
//
LOCUS       DAXWFK010000279         1676 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.283, whole genome shotgun sequence.
ACCESSION   DAXWFK010000279 DAXWFK010000000
VERSION     DAXWFK010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.283"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..>1676
                     /locus_tag="ACGEXS_002669"
     CDS             <1..>1676
                     /locus_tag="ACGEXS_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013617.1"
                     /translation="QVDSYNDDKNSNLNGAADIKIYELLDDKSKPTGQQTIIYQGTSN
                     EAINPNNPLKSSGFGDDWLQNAKLMNNDNESTDYLKQTDQLSNQYKIKLEDADRLSNS
                     DFLKKYRMESSNFKNKTIVADGGNSEGGAGAKYQGAKHPNEKVVATDSAMIPYAAWQK
                     FARPRFDNMISFNSTNDLLTWLQDPFIKDMPGKRVNINDGVPRLDTLIDSHVGYKRKL
                     NRKDNTYDTVPLIKIKSVKDTEIKNGKKVKKTINITLDMDGRIPINVWTGDSIARSGR
                     GTLIKLNLENLDALSKLITGETSGMLAECVIFLNESFNISENENKNFADRKKQLSEGF
                     KDKINLFQLEEMERTLISKINSLEEVADETIESISAVKHLLPDFALDALKERINELFK
                     GIKSFIEKVYDSIDNEILEIFKNIDHDFRDGVSEEMMKHLKVVKQNIDQIKNQNDIYG
                     RQIADIRSIMKQQDATILDGNFQINCSGENMVQGLVIPSNYLGRKMKILKDHIDDGIK
                     KIADYVQGIYDEYASKIVDVIKYLINTIPKIRKNLRHAIEMLNVKKKEFL"
BASE COUNT          713 a          180 c          305 g          478 t
ORIGIN      
        1 acaagtagac agttataatg atgataagaa tagtaatttg aatggtgctg ctgatattaa
       61 aatatatgag ttattagatg ataaaagtaa accaactggt caacagacaa taatttatca
      121 aggaacatct aatgaggcaa ttaatccaaa taatccatta aaatcatcgg ggtttggaga
      181 tgattggctc caaaatgcta aattaatgaa taatgataat gaaagcacag attatttaaa
      241 gcaaacagat caattatcaa atcaatataa aataaagtta gaagatgcag atagattatc
      301 aaatagtgat tttttaaaaa aatatagaat ggaatcaagt aacttcaaaa acaaaaccat
      361 tgtggcggat ggcggtaatt cggaaggcgg tgcaggagca aaatatcaag gagcgaaaca
      421 tccgaatgaa aaagttgttg ctactgactc agcaatgatt ccttatgctg cttggcagaa
      481 atttgctaga ccacgctttg ataatatgat tagttttaat agtaccaacg atttattaac
      541 atggttacaa gatccattca tcaaagatat gccaggaaaa cgcgttaaca ttaatgatgg
      601 tgtgcccagg ttagatactt taatagacag ccatgtaggt tataaaagga agttaaatag
      661 aaaagataac acatacgata ctgtaccgct aatcaaaata aagtcggtaa aagatacaga
      721 aattaaaaat ggaaaaaaag taaaaaagac tattaacata acattagata tggatgggcg
      781 aattccaata aatgtttgga caggagattc gattgcacgt tctggaagag gaactttaat
      841 taaacttaat ttagaaaatc ttgatgcgtt gagtaaactg attactggtg aaacaagtgg
      901 tatgttagca gaatgcgtaa tctttttaaa tgaaagtttt aacatctcag aaaatgaaaa
      961 taaaaatttt gcagatagaa agaaacaatt atcagaagga tttaaggata agattaactt
     1021 atttcagtta gaagaaatgg aaagaacttt aattagtaaa ataaactcac ttgaagaagt
     1081 tgcagatgaa acaatagaaa gtattagtgc tgttaaacac ttattacctg attttgcatt
     1141 ggatgcatta aaagaaagaa ttaatgagtt gtttaaaggt ataaaatctt ttatagaaaa
     1201 agtgtatgat agtatagata atgaaatttt agaaattttc aaaaatatag atcacgactt
     1261 cagagatgga gtatctgaag aaatgatgaa acatttgaaa gtagtgaaac agaatataga
     1321 ccaaataaaa aatcaaaatg atatttatgg taggcaaatt gcagatatta gaagtattat
     1381 gaaacaacaa gatgcaacaa ttttagatgg aaattttcaa attaattgta gcggcgaaaa
     1441 tatggtacag ggtctagtta taccttctaa ttatttagga agaaaaatga aaatattaaa
     1501 agaccatatc gatgatggta ttaaaaaaat agcagactat gttcaaggta tatatgatga
     1561 atatgcatcg aaaattgtcg atgtaataaa atatttgatt aatacaattc ccaaaatacg
     1621 taagaattta agacatgcaa ttgaaatgtt aaatgtaaaa aagaaagaat ttttgt
//
LOCUS       DAXWFK010000280         1670 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.284, whole genome shotgun sequence.
ACCESSION   DAXWFK010000280 DAXWFK010000000
VERSION     DAXWFK010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.284"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..588)
                     /locus_tag="ACGEXS_002670"
     CDS             complement(<1..588)
                     /locus_tag="ACGEXS_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1963 domain-containing protein"
                     /protein_id="HFF8013618.1"
                     /translation="MLNIQDVSHLSKKEQKAYNRFVESVENGNLPVLPCIEMDLKEMQ
                     EETLNQSKIGGMPFLKSFKDIPLDENNVPMVLLAQINLDDLPEQQELFPVKEGILQFW
                     ISSEDQMYGMSENLKGNNINSRLVYIKEPITDLSLENIQAHLKSLDADNEDIPFSGAF
                     SIEFRLSKQTITCTDYKYDEDVLALWNKVNPSFALK"
     gene            complement(767..1147)
                     /gene="gcvH"
                     /locus_tag="ACGEXS_002671"
     CDS             complement(767..1147)
                     /gene="gcvH"
                     /locus_tag="ACGEXS_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFF8013619.1"
                     /translation="MAVPNELKYSKEHEWVKVEGNVAIIGITEYAQSELGDIVFVELP
                     ETDDEINEGDTFGSVESVKTVSELYAPISGKVVEVNEELEDSPEFVNESPYEKAWMVK
                     VEISDESQIEALLTAEKYSEMIGE"
     gene            complement(1305..1661)
                     /locus_tag="ACGEXS_002672"
     CDS             complement(1305..1661)
                     /locus_tag="ACGEXS_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFF8013620.1"
                     /translation="MIKFYQYKNCTTCKKAAKFLDEYGVSYEPIDIVQHTPTINEFKT
                     IIANTGVEINKLFNTHGAKYRELDLKNKLQTLSDDEKLELLSSDGMLVKRPLAVMGDK
                     ITLGFKEDQYKETWLA"
BASE COUNT          506 a          317 c          197 g          650 t
ORIGIN      
        1 ttttagcgcg aaggatggat tgactttatt ccacaatgca agcacgtcct catcgtactt
       61 ataatcagta catgtaatag tttgtttcga caatctaaat tctatagaaa atgctccact
      121 gaacgggata tcctcattat cagcatctaa tgacttcaaa tgcgcttgaa tattttcgag
      181 tgataaatct gtaattggct cttttatata aacaagcctt gagtttatat tgtttccctt
      241 taaattttca gacattccat acatttgatc ttctgaacta atccaaaact gcaatatccc
      301 ttcttttaca ggaaataatt cttgttgttc tggaagatca tccaaattaa tctgtgctag
      361 caataccatt ggtacattat tttcatctaa tggtatatct ttaaaagatt ttaaaaatgg
      421 cattccacca atcttactct ggtttaatgt ttcttcttgc atctctttta gatccatttc
      481 aatacatggt agtactggta aattaccgtt ttctacagat tctacgaaac ggttatatgc
      541 tttttgctcc tttttagaaa gatgactaac gtcttgaatg tttagcatat taacttcccc
      601 caatttttat ttttaaaata gctaaattca ttatttccat cattcttgaa taataaaatg
      661 tagcaatact ccaaataata gtacctaata atttttcttt catatcattt attttctcag
      721 actaaaaata aactcctcaa tcttagatta aggagttaca cggtgattat tcaccaatca
      781 tttctgaata tttttcagct gttaataaag cttcaatctg actttcatca ctaatttcta
      841 cttttaccat ccatgctttt tcgtatggag attcatttac aaattcggga ctatcttcta
      901 gttcttcgtt gacttcaact actttaccag agattggtgc atataattct gatacagttt
      961 taactgattc tacgctacca aacgtatccc cttcattaat ttcatcatct gtttctggta
     1021 attcaacgaa aacaatatca cctaactcgc tttgtgcgta ttctgtgatt ccaattattg
     1081 ctacattacc ttcaacttta acccattcat gctcttttga atatttcaat tcatttggta
     1141 ctgccaagag aatcccctcc taattaatct ttacatcatt aatgatgcca taagtctaga
     1201 tgctattcaa ctatatttat aaaaattaac aattgtatag cttatttcat tttacatgta
     1261 atgaatctaa atatcgagat atttagtaaa tgcttacatt tcacttacgc taaccaagtc
     1321 tctttatatt gatcttcttt aaatcctaat gttatcttat cgcccattac tgctagagga
     1381 cgctttacta acataccatc agatgataac aactctaact tttcatcatc tgataaagtt
     1441 tgtaatttat ttttcaaatc aagctcacga tatttcgcgc cgtgtgtatt aaacaattta
     1501 ttaatttcta cgcctgtatt tgcaattatt gttttaaatt catttattgt aggtgtatgt
     1561 tgaacgatat caattggttc ataacttacg ccatattcat ctaaaaactt tgctgccttt
     1621 ttacaagttg tacaattctt atattggtaa aatttaatca tacgactgca
//
LOCUS       DAXWFK010000281         1653 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.285, whole genome shotgun sequence.
ACCESSION   DAXWFK010000281 DAXWFK010000000
VERSION     DAXWFK010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.285"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            22..210
                     /locus_tag="ACGEXS_002673"
     CDS             22..210
                     /locus_tag="ACGEXS_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013621.1"
                     /translation="MSLHFAILFWLALIFLVAATFILVLMKKTSKESKKESYLSFTVI
                     LYIFGFAILIYTFIFGVL"
     gene            398..967
                     /gene="lnsA"
                     /locus_tag="ACGEXS_002674"
     CDS             398..967
                     /gene="lnsA"
                     /locus_tag="ACGEXS_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein N-acylation protein LnsA"
                     /protein_id="HFF8013622.1"
                     /translation="MNYIKRTIILLILFVVVSPINSPKTIADNKYSEIQDDKFQLQPG
                     DIIVTKGPVMWGFFGHCSIAIDDKTILQIEGPGDKPTTQSFESFKYNYASGKNDWMKV
                     YRCSYPGAGKKAAEWVKKNYENTNHRYLVTLNLNSKKFTYCTKIIYQAYKFGVSEKSV
                     KSYGLHIISPYAIKDNFIDPYKLRLVKAY"
     gene            1055..1339
                     /locus_tag="ACGEXS_002675"
     CDS             1055..1339
                     /locus_tag="ACGEXS_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013623.1"
                     /translation="MNELTKEQKYTIAKFYKLYIERSNKGETETVANFFGDAKDAREN
                     YFCDRDYQDFLTNCQILIQNKYLTGEVLDDNIYNISILNKTFIEFEQNFG"
     gene            1401..1619
                     /locus_tag="ACGEXS_002676"
     CDS             1401..1619
                     /locus_tag="ACGEXS_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013624.1"
                     /translation="MSEWHINNQSTKPFLIQQAEKQLSIVKPLPNGSFQILTEINLEN
                     GNISNIDHSLLVSVDPKALEISIFDAVN"
BASE COUNT          599 a          242 c          241 g          571 t
ORIGIN      
        1 ggtatataag gagggtttca catgtcttta cattttgcaa ttctgttttg gctagcatta
       61 attttcttag ttgccgctac gtttatactc gtattaatga aaaaaactag caaagaatct
      121 aaaaaagagt cctatttaag tttcactgtc attctctata tttttggatt cgctatatta
      181 atatacacat ttatatttgg tgtgctataa atgaaattca ttttatatcc agtctgaaac
      241 gtctcaatga tgtttcagac tgttttttat tcttaataga catttcacac tctcatactt
      301 taatatttaa gatatgtttt agtcttacaa gcttattaaa gattacaatt ctaatttaag
      361 actatttaca tatatttctt caacaaggag cttttaagtg aactatatta aacgaacgat
      421 aattttactt atattattcg ttgtcgtatc cccaataaac tcaccaaaaa caattgcaga
      481 taataagtat tcagaaattc aagatgacaa attccaatta caacctggtg atattattgt
      541 tacaaaaggc cctgtcatgt ggggattttt tggtcattgt agtatcgcga ttgatgataa
      601 aacgatttta caaattgagg ggcctggcga caaaccaact acacaatcct tcgaatcttt
      661 taaatataat tatgcaagtg gcaaaaatga ttggatgaaa gtttatcgtt gcagctatcc
      721 tggtgcaggt aaaaaagcgg ctgaatgggt taagaaaaac tatgagaata caaatcatcg
      781 ttatctagtt acattaaatt tgaacagcaa aaaatttacg tattgtacta aaattatcta
      841 ccaagcttat aaatttggtg taagtgaaaa atcagttaaa agttatggac tacatattat
      901 ttcaccctat gcaattaaag ataattttat agatccatac aaactaaggc ttgttaaagc
      961 ttattaacac agtttcatca aatagttaaa ttagtttgtg ttttgcaata aattgggtat
     1021 agattacaaa tgatatttca ggaggcccaa tattttgaat gaactaacta aagaacaaaa
     1081 gtatacaata gctaaatttt acaaattata tattgaacgt tcgaataaag gagaaacaga
     1141 aacagtcgct aacttttttg gcgatgctaa agatgcacgt gaaaactatt tttgcgatcg
     1201 tgattatcaa gacttcttaa ctaactgcca aattttaatt caaaacaaat acttaactgg
     1261 tgaagttttg gacgataaca tttacaatat ttctatttta aacaagacat ttattgaatt
     1321 cgaacaaaat tttggttgaa catgaatgtc tcaactataa actttctaac cctattttaa
     1381 agcaggagtg aaaaacatac atgagtgaat ggcatattaa taatcaatct acaaaacctt
     1441 ttttaataca acaagcggaa aagcaattgt ctattgttaa accgttgcct aatggtagct
     1501 tccaaatact cacagaaata aatttggaga atggcaatat tagtaacatc gatcattctt
     1561 tacttgtttc agtagacccc aaagcattag aaataagtat atttgacgct gtaaattaaa
     1621 cgcaatattt ttatttaatt attttaatgt ttc
//
LOCUS       DAXWFK010000282         1615 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.286, whole genome shotgun sequence.
ACCESSION   DAXWFK010000282 DAXWFK010000000
VERSION     DAXWFK010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.286"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..1478)
                     /gene="cls"
                     /locus_tag="ACGEXS_002677"
     CDS             complement(<1..1478)
                     /gene="cls"
                     /locus_tag="ACGEXS_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFF8013625.1"
                     /translation="MRFTFSNDLGTLFTIILAIGFIINLVLAFIIIFLERNRRTASST
                     WAWLFVLFVLPLIGFILYLFFGRTVSARKLNKNNGNVLTDFDGLLKQQIESFDKGNYG
                     TDNKQVQKHHDLVRMLLMDQDGFLTENNKVDHFIDGNDLYDQVLKDIKNAKEYIHLEY
                     YTFALDGLGKRILHALEEKLKQGLEVKILYDDVGSKNVKMANFDHFKSLGGEVEAFFA
                     SKLPLLNFRMNNRNHRKIIVIDGQLGYVGGFNIGDEYLGLGKLGYWRDTHLRIQGDAV
                     DALQLRFILDWNSQAHRPQFEYDVKYFPKKNGPLGNSPIQIAASGPASDWHQIEYGYT
                     KMIMSAKKSVYLQSPYFIPDNSYINAIKIAAKSGVDVHLMIPCKPDHPLVYWATFSNA
                     SDLLSSGVKIYTYENGFIHSKMCLIDDEIVSVGTANMDFRSFELNFEVNAFVYDENLA
                     KDLRVAYEHDITKSKQLTKESYANRPLSVKFKESLAKLVSPI"
     gene            complement(1536..>1615)
                     /locus_tag="ACGEXS_002678"
     CDS             complement(1536..>1615)
                     /locus_tag="ACGEXS_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013626.1"
                     /translation="KEIEERTFPNNEEEFNAILTLSVNK"
BASE COUNT          544 a          293 c          203 g          575 t
ORIGIN      
        1 aaaattggcg aaactaattt tgctaacgat tctttgaatt taacagacag cggtctattg
       61 gcatatgatt ctttggttag ttgttttgat tttgtaatat catgttcata agccaccctt
      121 aaatctttag caagattttc atcatataca aaggcattta cttcaaaatt taattcaaaa
      181 cttctaaagt ccatatttgc tgtgcccact gatacgattt catcatcaat taagcacatt
      241 ttagaatgta taaatccatt ttcatacgta taaattttaa caccacttga taataagtca
      301 gaggcatttg aaaatgtcgc ccaatatact aatggatgat ctggcttaca tggaatcatt
      361 aaatgtacat ctacacctga tttagcagca attttaatgg catttatata tgaattatcc
      421 ggaatgaaat atggtgattg taaatataca gatttctttg cactcataat catttttgta
      481 taaccgtatt caatttgatg ccagtcacta gccgggccac ttgcagctat ttgaattggt
      541 gaattgccca atggtccgtt ctttttaggg aaatacttaa catcatattc aaattgtgga
      601 cggtgcgctt gcgaattcca gtctaaaata aatcgcaact gcagtgcatc aaccgcatcc
      661 ccttgtatac gtaaatgcgt atctctccaa tatcctaatt ttcctaatcc tagatattca
      721 tcaccaatgt taaatcctcc gacataacct agttgaccat cgattacgat gatttttcta
      781 tgatttctat tattcattct gaaattcaat aacggtaatt ttgaagcaaa aaatgcttca
      841 acttctccac ctaacgattt aaaatgatca aaatttgcca tcttaacatt tttagatcca
      901 acatcatcat ataatatttt tacttctaga ccttgtttca atttttcttc taaagcatgt
      961 aaaattcttt tacctaaacc atctaaagcg aaagtatagt actctaaatg gatatattct
     1021 tttgcatttt taatatcttt taaaacttga tcatataaat catttccatc aatgaaatga
     1081 tcaactttat tattttcagt taaaaaacca tcttgatcca tcaaaagcat acgtactaaa
     1141 tcatgatgtt tttgaacttg tttgttatca gtaccataat tacctttatc aaagctttct
     1201 atttgttgtt ttaaaagtcc atcgaaatcc gttaacacgt taccattgtt tttattcaat
     1261 ttgcgtgccg aaacggttct accaaaaaac aagtaaagaa taaaaccaat caatggtaag
     1321 acaaaaagta caaatagcca tgcccaagtt gaactcgctg tacgcctatt tctttctaaa
     1381 aagataataa taaaagccaa tactaaatta atgatgaatc caatggctaa aataatagta
     1441 aataacgttc ctaaatcgtt tgaaaatgta aatcgcatag tcttttctcc tataaagaaa
     1501 ggcacaaaaa aacattttgc acctttcacg tcatattatt tattcacaga taaagttaaa
     1561 attgcattga attcttcttc attatttggg aatgttcttt cttctatttc tttaa
//
LOCUS       DAXWFK010000283         1611 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.287, whole genome shotgun sequence.
ACCESSION   DAXWFK010000283 DAXWFK010000000
VERSION     DAXWFK010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.287"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            140..1594
                     /locus_tag="ACGEXS_002679"
     CDS             140..1594
                     /locus_tag="ACGEXS_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFF8013627.1"
                     /translation="MEDNKMNRSLNSRHISMIAIGGAIGTGLFVATGNIISQAGPGGA
                     ILAYLVIGVMLYFLMSSIGELATFYPVSGSFSSYSTRFIDSSLGFTMGWLYWALWSLV
                     TSVDVIVASNVLYFWDTFKFFHPITWSLIFITILLLLNIFSVKSFGETEFWLSLIKVL
                     TIIVFVIFGFLMIFGILGGHTYGFENYTKGQAPFVGGISGFLGVLLVAGFSVGGTEVV
                     AVTAGESDDPKKSMPKAIKQVFWRILLFYVLSIAVIGAIIPYTDPSLLRASSSISQSP
                     FTIVFDRVGIAFAASVINAVILTSLLSAANSGVYTTGRMLYSLSSDKKAPQFLSKLNK
                     TTKLPLRALLTTYAVVVIVIIYANFNSNAVFNLLEIIGSMIIVVWGSSIWSQIRLRQA
                     IKKQGQDPNKVLPYKAPFYPLGPIIVITTLLFLLFGGSVEYILKDQWLNAFKNFLPLI
                     ILALIYFIHKIIHKTKFVKLETINLKPHDYDNQK"
BASE COUNT          510 a          255 c          266 g          580 t
ORIGIN      
        1 attacaatta aacatgctca agatgattaa aaaatcagaa aattttaatt ttttcgcttt
       61 acagtactaa atagttattg aatagtacac ttaaaaagct tacaataatg ggtatattta
      121 aatagatggg agactaaata tggaagataa taaaatgaac cgtagtctta actcaagaca
      181 catttccatg attgctatag gtggtgcaat tgggactggt ctatttgtag ctactggtaa
      241 tatcatttct caagctggtc ctggaggcgc tatactcgct tatcttgtta ttggtgtcat
      301 gctatatttc ttaatgtcat caattggaga gttggcaaca ttttatccag tatcaggttc
      361 attcagctct tattcaacac gctttattga ctcatctctt ggctttacca tgggatggtt
      421 gtattgggca ttgtggtcat tagttacaag tgttgatgtc atagtagcgt caaatgtgct
      481 ttatttctgg gacacattta aatttttcca ccccattact tggagcttaa tctttattac
      541 aattttacta ttattaaaca ttttttctgt aaaatcattt ggagaaactg agttttggtt
      601 atcattgatt aaagtgttaa caattatcgt attcgttatt tttggctttt taatgatttt
      661 cggtatctta ggtggtcata catatggatt tgaaaactat acaaaaggcc aagcaccgtt
      721 tgttggtggt atctctggtt tcttaggcgt attattagtc gccggatttt cggttggtgg
      781 tacagaagta gtagcagtaa ctgctggtga atcagatgac cctaaaaagt ctatgcctaa
      841 ggcaattaaa caagtatttt ggcgtattct tttattctat gtcttatcaa ttgcagtaat
      901 tggtgcaatt attccgtaca cagatccatc attattaaga gcaagtagtt caataagtca
      961 aagcccattt acaattgtat tcgatagagt aggcatagcc tttgcagcat cagtaatcaa
     1021 cgcggttatt ttaacttcat tattatccgc tgcaaattca ggtgtttata caacaggcag
     1081 aatgttgtat tccttaagtt cagacaaaaa agcaccccaa tttttaagta aattaaacaa
     1141 gacaactaag ttacctttaa gagcattatt aactacttat gcagtcgttg ttattgttat
     1201 tatttatgca aactttaatt caaatgccgt ttttaattta cttgaaatta ttggttcaat
     1261 gattatagtt gtttggggat caagcatttg gtcacaaata cgattgcgac aagctattaa
     1321 aaaacaaggt caagacccta ataaggtcct accatataaa gcaccttttt atccattagg
     1381 accaatcatt gtcatcacta cactattatt cttgctattt ggtggctcag ttgaatatat
     1441 tttaaaagat caatggttaa atgcttttaa aaacttttta cctttaatca ttctagcgtt
     1501 gatttacttt attcataaaa tcattcacaa aacaaaattt gtaaagctag aaacaattaa
     1561 tttaaaacca cacgattatg acaatcaaaa ataaataaat tctcatacgg c
//
LOCUS       DAXWFK010000284         1608 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.288, whole genome shotgun sequence.
ACCESSION   DAXWFK010000284 DAXWFK010000000
VERSION     DAXWFK010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.288"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..229
                     /locus_tag="ACGEXS_002680"
     CDS             <1..229
                     /locus_tag="ACGEXS_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFF8013628.1"
                     /translation="LVIKKLYGYSTLRANYQYLLINNIVVIFIGILTNVILHSHYIMM
                     LFATILVVQILLQICSLYYHGRRFNEVIKEF"
     gene            232..552
                     /locus_tag="ACGEXS_002681"
     CDS             232..552
                     /locus_tag="ACGEXS_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFF8013629.1"
                     /translation="MKFIIAILLGLILSITIAFTIIHHPILDRFNPFLKTEYSYAKVP
                     KGTQQYVNITAYSERGEKLDYKLTFNGFSPSRTYVEIKHKGQYVISITYVEKEDTPKE
                     VRQE"
     gene            549..1190
                     /locus_tag="ACGEXS_002682"
     CDS             549..1190
                     /locus_tag="ACGEXS_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8013630.1"
                     /translation="MIELKDLTIQKGNTHILKKLNLKFQCGKSYALIGKSGCGKSTLL
                     NTIAGLEKTGKQYVYFNGQLEQFKSNFYRDKLGYLFQNYGLIDNLTVNENLDIGLAYK
                     KISKKEKEQIKIRYIEQFGLSNSLKRKVHTLSGGEQQRVALIRMMLKDPIVMLADEPT
                     GALDPKTGQMIIQSLFGLVDENKVLILATHDMAIANQCDEIIDLEQYRKVAYM"
     gene            complement(1278..1568)
                     /locus_tag="ACGEXS_002683"
     CDS             complement(1278..1568)
                     /locus_tag="ACGEXS_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013631.1"
                     /translation="MFYACHFKVKIGGVILTRTYNIIGILSCLISFIIMALPMIWYTA
                     SALWFFPGAIMILLLSLVIVFCYIKTKNQLHLLLIVLNIIILLFFSLPLLLS"
BASE COUNT          650 a          200 c          253 g          505 t
ORIGIN      
        1 actcgtaatc aaaaagctat atggttattc aacattaaga gccaattacc aatacttatt
       61 aataaataat atagttgtta tttttattgg aatattgacg aatgtaattt tacattctca
      121 ctatataatg atgttatttg caacgattct tgtcgttcaa atattattgc agatttgcag
      181 cctatactat catggccgac gttttaatga agttatcaag gagttttaac tatgaaattt
      241 atcattgcaa tattattagg attaatatta tccattacta ttgcttttac aatcatacat
      301 catcctatac ttgatcgttt taatcctttc ttaaaaacgg agtatagtta tgccaaagtg
      361 ccaaaaggta cgcaacaata tgttaatatt acggcttata gtgaaagagg ggaaaagctt
      421 gattataaat taacatttaa tggattttca cctagtagaa cgtatgtcga aataaagcat
      481 aaagggcaat atgtcatatc gatcacatat gttgaaaaag aggatacacc aaaagaggta
      541 agacaagaat gatagaatta aaagatttaa ccatacaaaa aggtaataca catattttga
      601 agaaacttaa tttgaaattt caatgtggaa aatcatatgc acttattggt aaaagtggat
      661 gcggtaaatc tacattatta aatactattg ctggacttga aaaaacagga aaacaatatg
      721 tctattttaa tggtcaatta gaacaattta aatctaattt ttacagagat aaattaggat
      781 atttatttca aaattatgga ttaatcgata atttgacagt aaatgaaaat ttagatattg
      841 gattagcata taaaaaaata agtaagaaag aaaaagaaca aataaagata cgttatatag
      901 aacagtttgg tctgtcaaac agtttaaaaa gaaaagttca tacgctaagt ggaggtgaac
      961 aacaacgtgt cgctttaatt agaatgatgt taaaagatcc gattgttatg ttagctgatg
     1021 aaccaacggg tgcgttagat cctaaaacag gacagatgat tattcaatca ttatttggtt
     1081 tggtcgatga aaataaagtg ctgattttag caacacatga tatggctatt gcaaatcaat
     1141 gtgacgaaat aatagattta gaacagtata gaaaagtagc atatatgtga tatccaaaag
     1201 ataaaattaa aagccaatca atgggaaatg ttgacttcaa cactccgttt gattggcttt
     1261 tatactgtgt tactaagtta tgaaagtaac aacggcaaac tgaaaaaaag caatataata
     1321 atatttaata cgattagtaa taaatgtaat tgatttttcg ttttaatata gcaaaaaaca
     1381 atgactaaag ataacaacaa tatcatgatt gcacctggga agaaccataa tgctgaagct
     1441 gtgtaccaaa tcattggtaa cgccataata ataaaagata taagacaaga aaggatacca
     1501 ataatattat aagttctagt caaaattaca cctccaattt tcactttaaa atgacatgcg
     1561 taaaacaatc attttaaata cattatacaa taaaattaaa ttgtgcgg
//
LOCUS       DAXWFK010000285         1594 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.289, whole genome shotgun sequence.
ACCESSION   DAXWFK010000285 DAXWFK010000000
VERSION     DAXWFK010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.289"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..351
                     /locus_tag="ACGEXS_002684"
     CDS             <1..351
                     /locus_tag="ACGEXS_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HFF8013632.1"
                     /translation="SEDLNYYTDIPKEYNVSVQVFDDLWMDLYDLFKNLRNLFKEEGL
                     EPWTSCEFDFTRDGKLNVSFDYIDWANSEFGQMGREHYYMYKKFGIWPEKEYAINWVK
                     KIKDYVKEQDEAEL"
     gene            362..862
                     /locus_tag="ACGEXS_002685"
     CDS             362..862
                     /locus_tag="ACGEXS_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HFF8013633.1"
                     /translation="MTFEEKLSQMYNEIANEISGMIPVEWENIYTIAYVTDQGGEVIF
                     NYTKPGSDELNYYTYIPREYNVSEKVFYDLWTDLYRLFKKLRETFKEEGLEPWTSSEF
                     DFTSEGKLKVSFDYIDWINTEFDQLGRENYYMYKKFGVLPEMEYEMEEVKEIEQYIKE
                     QDEAEL"
     gene            873..1361
                     /locus_tag="ACGEXS_002686"
     CDS             873..1361
                     /locus_tag="ACGEXS_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin YezG family protein"
                     /protein_id="HFF8013634.1"
                     /translation="MTFEEKLSQMYNEIANEISGMIPVEWEKVYTIAYVDDEGGEVVF
                     NYTKPGSEDLNYYSDIPKDCNVSKDIFKNSWFKVYRMFDELRETFKKEDLEPWTSCEF
                     DFTRKGNLKVSFDYIDWIKLGFGPSGKENYYMYKKFGILPDMEYEMEEIRAVEKYVKE
                     QE"
BASE COUNT          630 a          154 c          318 g          492 t
ORIGIN      
        1 agtgaagatt tgaattatta taccgatata cctaaggagt ataatgtttc tgtgcaagta
       61 tttgatgatt tatggatgga tttatatgat ttgtttaaga atttaagaaa tttatttaaa
      121 gaagaaggac ttgaaccatg gacatcatgt gaatttgact ttacaagaga cggcaaattg
      181 aatgtttcat ttgattatat tgattgggcg aattcagagt ttggacaaat gggaagagaa
      241 cattattaca tgtataaaaa atttggaatt tggcctgaaa aagaatatgc cataaattgg
      301 gtaaaaaaaa taaaagatta tgttaaagag caagatgaag ctgaactata ggggcgataa
      361 tatgactttc gaagaaaaac taagtcaaat gtacaatgaa attgcaaatg aaatcagtgg
      421 aatgatacca gttgaatggg aaaatatata tacaattgcc tatgtaactg atcaaggtgg
      481 agaggtcatt tttaattata ctaaaccagg tagcgatgaa ttgaattatt acacatatat
      541 ccctagagag tataatgtct ctgaaaaagt attttatgat ttgtggacgg atttatatag
      601 attgtttaag aagttaagag aaacttttaa agaagaaggg cttgaaccat ggacatcaag
      661 tgaatttgac tttacaagcg aaggtaaatt aaaagtttca tttgattata ttgattggat
      721 aaatacagag tttgatcaat taggccgtga aaactattat atgtataaaa agtttggtgt
      781 tttaccagaa atggaatacg aaatggaaga agttaaagaa atcgagcaat atattaaaga
      841 gcaagatgaa gctgaactat aggggcgata acatgacttt cgaagaaaag ctaagtcaaa
      901 tgtacaatga aattgcaaat gaaatcagtg gaatgatacc agtagaatgg gaaaaagtat
      961 atacaattgc ctacgtagat gatgaaggtg gagaggttgt ttttaattat actaaaccag
     1021 gaagtgaaga tttgaattat tattcagata ttcctaaaga ttgcaatgtc tcaaaagata
     1081 tttttaagaa ttcatggttt aaagtttatc gaatgtttga tgagttaaga gaaactttta
     1141 aaaaagaaga tttagaaccg tggacatcat gtgaatttga ctttacaaga aagggaaatt
     1201 taaaagtatc atttgattat atagattgga ttaaattagg ttttggccca tcaggaaagg
     1261 aaaactacta tatgtacaaa aaatttggta ttttaccaga tatggaatat gaaatggaag
     1321 aaattcgagc agtagagaag tatgttaaag agcaagagta gcagacatgt tataaaagac
     1381 tgtgcaaaat caccctcgtt ttacatttga ttcaaagaag aaggtaaaag ataagattat
     1441 ttgcaactta aaaagtcaat tagcttatcg gtatccatac atcatggata aatgagttca
     1501 actaattaac aaatcacgat ataaatttaa aattgtcatt aattgaaaag gttattgtag
     1561 tgtattttat aaagttgatg tacatcgact tatt
//
LOCUS       DAXWFK010000286         1566 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.290, whole genome shotgun sequence.
ACCESSION   DAXWFK010000286 DAXWFK010000000
VERSION     DAXWFK010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.290"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            242..967
                     /locus_tag="ACGEXS_002687"
     CDS             242..967
                     /locus_tag="ACGEXS_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL14"
                     /protein_id="HFF8013635.1"
                     /translation="MKKNIMNKLVLSTALLLLETTSTQLPKTPISFSSEAKAYNISEN
                     ETNINELIKYYTQPHFSLSGKWLWQKPNGSIHATLQTWVWYSHIQVFGSESWGNINQL
                     RNKYVDIFGTKDEDTVEGYWTYDETFTGGVTPAATSSDKPYRLFLKYSDKQQTIIGGH
                     EFYKGNKPVLTLKELDFRIRQTLIKNKKLYNGEFNKGQIKITADGNNYTIDLSKKLKL
                     TDTNRYVKNPRNAEIEVILEKSN"
     gene            1062..>1566
                     /locus_tag="ACGEXS_002688"
     CDS             1062..>1566
                     /locus_tag="ACGEXS_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein"
                     /protein_id="HFF8013636.1"
                     /translation="MNNNITKKIILSTTLLLLGTASTQFPNTPINSSSEAKAYYINQN
                     ETNVNELTKYYSQKYLTFSNSTLWQKDNGTIHATLLQFSWYSHIQVYGPESWGNINQL
                     RNKSVDIFGIKDQETIDSFALSQETFTGGVTPAATSNDKHYKLNVTYKDKAETFTGGF
                     PVYEGNKP"
BASE COUNT          603 a          247 c          226 g          490 t
ORIGIN      
        1 caattatcaa acaatttaat acaattgatt agagaataat tctaacgtat cgtttttaat
       61 atataactcc cgcctacttt attagatact gtttctgtcc aaaacttaaa aatgataagt
      121 tttgcttaaa taacactact aactgtttaa gtttatttaa catagtttta gcttttattt
      181 aattccgaat cggtgtaata gcttatatac tttgggtaat tcacgcaaag gagattttca
      241 tatgaaaaag aacatcatga ataaattagt tttatcaaca gcattgttac ttttagaaac
      301 tacatcaaca caacttccta aaacaccaat cagtttttca tctgaagcaa aagcctataa
      361 tatcagtgaa aacgagacta atatcaatga actaatcaaa tattacactc agccgcattt
      421 ttcattatct ggaaaatggt tatggcaaaa gcccaatggt agcattcatg caacattgca
      481 aacgtgggtt tggtatagtc atattcaagt gtttggatcc gagagttggg gaaacattaa
      541 tcagttaaga aataaatacg ttgatatatt tggaactaaa gatgaggaca cagttgaagg
      601 ttactggact tatgatgaaa catttactgg tggtgttacg ccagcagcta cttcatctga
      661 taagccttat agactatttt taaaatatag tgataaacaa caaactatca tcggtggaca
      721 tgaattttac aaaggaaata aaccagtatt aactttaaaa gaattagatt tccgtattcg
      781 tcaaacatta ataaaaaata aaaagttata taacggagaa tttaataaag gtcaaattaa
      841 gataactgct gatggaaata attacacgat tgatttaagt aaaaagttaa aattaactga
      901 cacaaaccgt tatgttaaaa atcctcgtaa tgcagaaatt gaagtcatac tcgaaaaatc
      961 taactaacct attacctttt gtaaatgcgg ataatttcaa ttatctaatt aacccctttt
     1021 tataattaaa cattccaaca atactcaaag gagaaattcg aatgaacaat aacatcacga
     1081 aaaaaattat tttatcaaca acattgttac tattaggtac agcatctaca caatttccta
     1141 atacacctat caattcttca tctgaagcga aagcttatta tataaatcaa aacgaaacta
     1201 acgttaatga gttaactaaa tattactcgc aaaaatattt aaccttctct aacagtacgt
     1261 tatggcaaaa agataacggt acgattcatg caacgttgtt acagttttct tggtatagtc
     1321 atattcaagt ttatggacct gaaagttggg gcaatatcaa ccaattaaga aataaaagcg
     1381 ttgatatttt tggcataaaa gaccaagaaa ccattgattc ttttgcatta tctcaagaaa
     1441 cgtttactgg tggtgttact cctgcagcaa catctaacga taaacactat aaactgaatg
     1501 taacatataa agataaagca gaaacgttta ctggcggatt tccagtttat gaaggcaata
     1561 agcctg
//
LOCUS       DAXWFK010000287         1552 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.291, whole genome shotgun sequence.
ACCESSION   DAXWFK010000287 DAXWFK010000000
VERSION     DAXWFK010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.291"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(262..335)
                     /locus_tag="ACGEXS_002689"
     tRNA            complement(262..335)
                     /locus_tag="ACGEXS_002689"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(299..301),aa:Arg,seq:tct)
     gene            699..833
                     /locus_tag="ACGEXS_002690"
     CDS             699..833
                     /locus_tag="ACGEXS_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class phenol-soluble modulin"
                     /protein_id="HFF8013637.1"
                     /translation="MEGLFNAIKDTVTAAINNDGAKLGTSIVSIVENGVGLLGKLFGF
                     "
     gene            890..1024
                     /locus_tag="ACGEXS_002691"
     CDS             890..1024
                     /locus_tag="ACGEXS_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class phenol-soluble modulin"
                     /protein_id="HFF8013638.1"
                     /translation="MTGLAEAIANTVQAAQQHDSVKLGTSIVDIVANGVGLLGKLFGF
                     "
     gene            1148..>1552
                     /locus_tag="ACGEXS_002692"
     CDS             1148..>1552
                     /locus_tag="ACGEXS_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HFF8013639.1"
                     /translation="MGKLGYKNILIDFDDTIVDFYDAEEWAFHYMANVFNHKATKDDF
                     LTFKKINHQHWEAFQQNKLTKSEVLSERFVNYFKHHQMEVDGHRADVLFRNGLAEAKV
                     KYFDQTLETIVELSKRHDLYIVTNGVTETQKRR"
BASE COUNT          563 a          209 c          284 g          496 t
ORIGIN      
        1 tagttcaggt gcagtataga tgaaatttga gcacaaaatt acttaattaa aaatgtataa
       61 acaagtgtca gacgcgtgtc aattgtgtgt caaaaaattc tataacgaat aagcatgctt
      121 gttttaatta ctgtattttc ggggcttatg cgggggacgt tttggtgacg catcatacta
      181 tattactgaa attcaaaaca aaaagagccc cgtaatcacg gaactctttt gtttggtaat
      241 gcgtataaaa atacctataa acgtcctggg agggattcga acccccgacc gatggcttag
      301 aaggccattg ctctatccag ctgagctacc aggacacgtt taacaacaca agaattatta
      361 tatctaaatg aacttaaatt agcaatacct tgtaaataaa aaatgtttat atttttcact
      421 attatagagc tatttatcta aaaaggttca ataagactta aatacgaatt caggcaactt
      481 aattgtgtta aatacagttt tgaatgccta actgtatttc ttttctcttt aaaatacagt
      541 taagtacatt ataagatgtt gtgcggataa acaaactaat tgcatcaaat ttattttaaa
      601 ataacaacaa caaaacgtta agcgaataac atttcggtga tttaaaagct acgcacgttt
      661 ttgttatctt caaatttaaa ttttaaggag tgttttcaat ggaaggttta tttaacgcaa
      721 ttaaagatac cgtaactgca gcaattaata atgatggcgc aaaattaggc acaagcattg
      781 tgagcatcgt tgaaaatggc gtaggtttat taggtaaatt attcggattc taatttcaat
      841 atgttatgta agtaatcagt attatttcaa aggtgaggga gagatttaaa tgactggact
      901 agcagaagca atcgcaaata ctgtgcaagc tgcacaacaa catgatagtg tgaaattagg
      961 cacaagtatc gtagacatcg ttgctaacgg tgtgggttta ctaggtaaat tatttggatt
     1021 ctaatataat aactaatatt ctttaaaata aactgggtga gcatacttta atgttatgca
     1081 ctcagtttat tttatttgca gaaatttgag cctctgttaa gatttagata catagacaat
     1141 ataggagatg gggaaattgg gatataaaaa tattttgata gactttgatg atacaattgt
     1201 tgatttttat gatgcagaag aatgggcgtt tcactatatg gcgaatgttt ttaatcataa
     1261 agcaacaaag gatgattttt taacatttaa aaaaatcaat caccaacatt gggaagcttt
     1321 tcaacaaaat aaattaacga agtctgaagt attatcagaa cgatttgtga attacttcaa
     1381 acatcatcaa atggaagttg atgggcatcg tgcagatgtg ttatttagaa atggattagc
     1441 agaagctaaa gttaaatact ttgatcaaac attagaaaca attgtcgaat tatcgaaaag
     1501 acatgattta tatattgtta ctaatggtgt aaccgaaacg caaaagagaa gg
//
LOCUS       DAXWFK010000288         1545 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.292, whole genome shotgun sequence.
ACCESSION   DAXWFK010000288 DAXWFK010000000
VERSION     DAXWFK010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.292"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(297..1388)
                     /locus_tag="ACGEXS_002693"
     CDS             complement(297..1388)
                     /locus_tag="ACGEXS_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase"
                     /protein_id="HFF8013640.1"
                     /translation="MSNKLESYRSEIVSLNHQILDLLSKRGELAQKIGEEKLKQGTRI
                     YDPQREKEMLNDLIDSNKGPFNDNTIKQLFKEIFKASTDLQKSENEKHLYVSRKLKPE
                     DTIVTFDNGGIIGDGNKSFVFGPCSVESFEQVEAVAKNLHAKGEKFIRGGAFKPRTSP
                     YDFQGLGVEGLKILKQIKDKYDLNVVSEIVNPNDFEVADEYLDVFQIGARNMQNFELL
                     KEAGRTKKPILLKRGLSATIEEFVYAAEYIASQGNQNIILCERGIRTYEKATRNTLDI
                     SAVPILKQGTHLPVMVDVTHSTGRKDIMLPTAKAALAVGADGVMAEVHPDPSVALSDA
                     GQQMDLDEFQAFYDELKPLADLYNAKKLK"
BASE COUNT          493 a          272 c          210 g          570 t
ORIGIN      
        1 atgttcacat ttcagcaaca aatgtgtgaa tctattatat tttacagata attcatagtt
       61 ataattttaa ttactgctat cagtcataat ctttaataat tgtgaaattg aacaagtgtc
      121 gaagtattat aacaatcgtt aagtgcagta tacctttatt tctatacaaa aaataatagc
      181 cactctaaaa gtaatatact cttagaatgg ctgtaagttt aaaattgtcg attcaaagat
      241 aatattctac ttcaacatga catattagtt aagtagtcta tagtttcctt ggaatattat
      301 tttaactttt tagcgttata taaatcagct aaaggcttta attcatcata aaatgcttgg
      361 aattcatcta aatccatttg ttgacccgca tcactaagtg caacagatgg atctggatgc
      421 acctcagcca taactccatc agcaccaact gctaatgctg ctttcgcagt tggtaacatg
      481 atatctttac gacctgtact atgcgtaaca tctaccatga ctggtaagtg tgtaccttgt
      541 tttaaaattg gtactgctga aatatctaaa gtgttacgtg tcgccttttc ataagttcgg
      601 attccacgtt cacataaaat aatgttttga ttaccttgtg aagcaatgta ttcagctgca
      661 taaacaaact cttcgattgt agcagataaa ccacgtttta atagaatagg ctttttcgta
      721 cggccagctt cttttaataa ctcgaagttt tgcatattac gtgcaccaat ttggaatacg
      781 tctaaatact catcagccac ttcaaaatca tttggattta cgatttcgct gacaacattt
      841 aaatcatatt tatctttaat ctgtttaagt attttaagtc cttcaacacc taggccttgg
      901 aaatcatatg gtgatgtacg tggtttaaat gcaccgccac gaataaattt ttcaccttta
      961 gcatgtaagt ttttagcaac agcttcaact tgttcaaatg attcaactga acatggccca
     1021 aatacaaatg atttattgcc gtctccaata atgcccccat tatcaaatgt tacaatcgta
     1081 tcttcaggtt tcaacttacg tgatacatat aaatgttttt cattttcaga tttttgtaaa
     1141 tctgtagagg ctttgaaaat ttctttaaat aattgcttaa tagtattatc gttgaatggt
     1201 cctttgttac tatcgattaa gtcgttaagc atttcttttt cacgttgtgg atcataaata
     1261 cgtgtacctt gttttaattt ttcttcccca attttttgtg ctagttcacc acgtttagat
     1321 aataagtcta aaatttgatg attcagtgat acaatctcac ttctgtatga ttctaattta
     1381 ttactcatcc ttactcacct ctactaatta tttcaagtat attccacttt aaagcaataa
     1441 aatataatgt taaaaaatat tctaacaata tcatatgtat agttcaaccc taaattgaca
     1501 gaaaataatc aattttctaa ttatatgatt cgagactgac atttc
//
LOCUS       DAXWFK010000289         1536 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.293, whole genome shotgun sequence.
ACCESSION   DAXWFK010000289 DAXWFK010000000
VERSION     DAXWFK010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.293"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            65..1129
                     /locus_tag="ACGEXS_002694"
     CDS             65..1129
                     /locus_tag="ACGEXS_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HFF8013641.1"
                     /translation="MLTVDQVKELVGEIKDPIIDVPLKETEGIVEVSIKEEKEHVSVK
                     LAMAQLGGAPQLDLQMAVVNALKENGAKTVGIRFETLPEEKVNQFKPKEENKPKTIEG
                     LLSQNNPVEFIAIASGKGGVGKSTVAVNLAVALAREGKKVGLVDADIYGFSVPDMMGI
                     DEKPGIKGKEVIPVERHGVKVISMAFFVEENAPVIWRGPMLGKMLTNFFTEVKWGDIE
                     YLILDLPPGTGDVALDVHTMLPSSKEIIVTTPHPTAAFVAARAGAMAKHTDHSILGVI
                     ENMSYFESKETGNKEYVFGKGGGTKLADELNTQLLGELPLEQPSWNPKDFAPSIYQSD
                     DRLGKIYSSIAQKVIAATNK"
     gene            1218..1403
                     /locus_tag="ACGEXS_002695"
     CDS             1218..1403
                     /locus_tag="ACGEXS_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013642.1"
                     /translation="MLSNKRVEKEAGCEHKKFLKKLLTLNSKVDNMIKSSKTTQLFLK
                     KNFKSVKLTIEITIKTI"
BASE COUNT          573 a          195 c          317 g          451 t
ORIGIN      
        1 gactaagcaa aaattgttat ttgctatgat gtagatgtct taaaatgatt aagggggatt
       61 tgctttgtta acggtagatc aagtgaaaga attggtagga gaaattaaag atcctattat
      121 agatgtgcct ttaaaagaaa cagaaggtat tgttgaagtt tctattaagg aagaaaaaga
      181 acatgtgagt gttaaattag caatggcaca attaggtggt gcaccgcaat tagatttaca
      241 gatggctgtt gttaatgcat taaaagaaaa cggtgcgaaa acggtcggta tacgatttga
      301 aacattgccg gaagaaaaag taaatcaatt taaaccaaaa gaagaaaata aacctaaaac
      361 gatagaaggc ctattatctc aaaataatcc agtcgaattt attgcaatag cctccggtaa
      421 aggtggtgtc ggtaaatcta ctgttgcagt aaatttagcc gttgccttag ctcgtgaagg
      481 gaaaaaagtc ggattagtag atgccgatat atatggattt agtgtaccag atatgatggg
      541 tattgatgaa aagcctggaa ttaaagggaa ggaagtaatt ccagttgaac gtcatggcgt
      601 taaagttata tcaatggcct tttttgtgga agaaaatgcg ccagttatat ggagagggcc
      661 aatgttaggt aaaatgttga cgaatttctt tacagaagtt aaatggggag acattgaata
      721 tttaatactc gatcttccac ctggaacagg agatgtagct ttagatgttc atacgatgtt
      781 accttcaagt aaggaaatta ttgtaacgac acctcatcct acagcagcat ttgttgcagc
      841 tcgcgcaggt gcgatggcaa aacatacgga tcattctatt cttggcgtaa ttgaaaacat
      901 gagttatttt gaaagtaaag agacgggtaa taaagaatat gtctttggca aaggtggcgg
      961 tactaagtta gctgatgaac ttaatactca attacttggg gaattacctt tagagcaacc
     1021 atcttggaat ccaaaagatt ttgcaccgtc aatatatcaa tcagatgatc gtctaggtaa
     1081 aatttatagc tcgattgcac aaaaagttat agctgctaca aataaatagg tataatatat
     1141 gctaaacgta caaaattact tttattttgt acgtttttat gttgatttgt tttggaatgc
     1201 tggtaaaata agttattgtg ttaagcaata aacgcgttga aaaagaagcg ggttgtgaac
     1261 acaaaaagtt tttaaaaaag ttattgactt taaattccaa agttgataat atgataaagt
     1321 cgtcaaaaac gacgcagttg tttttaaaga agaatttcaa aagtgtaaaa cttaccattg
     1381 aaataactat aaaaacaata tagaataaat aaagtaatcg ttcaaaaagt tattgacttc
     1441 aagaaataaa tgaagtagaa taaaagagat tgaacattaa tgaacattga aaactgaatg
     1501 acaatatgtc aacgttaatt ccaaaaacgt aactat
//
LOCUS       DAXWFK010000290         1523 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.294, whole genome shotgun sequence.
ACCESSION   DAXWFK010000290 DAXWFK010000000
VERSION     DAXWFK010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.294"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(308..>1523)
                     /locus_tag="ACGEXS_002696"
     CDS             complement(308..>1523)
                     /locus_tag="ACGEXS_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFF8013643.1"
                     /translation="FSEKEKIQLLADIVELQTENNNEIDVCNYLTDLFDKYDIKSEIL
                     KVNEHRANIVAEIGNGSPILALSGHMDVVDAGNQDNWSYPPFQLTEKDGKLYGRGTTD
                     MKGGLMALVVSLIELKEQNELPHGTIRLLATAGEEKEQEGAKLLADKGYLDDVDGLII
                     AEPTGSGIYYAHKGSMSCKVTATGKAVHSSVPFIGDNAIDTLLEFYNLFKEKYSELKQ
                     QDTKHELDVAPMFKSLIGKEISEEDANYASGLTAVCSIINGGKQFNSVPDEASLEFNV
                     RPVPEYDNDFIESFFQNIINDVDSNKLSLDIPSNHRPVTSDKNSKLITTIKDVASSYV
                     EQDEIFVSALVGATDASSFLGDNKDNVDLAIFGPGNPLMAHQIDEYIEKDMYLKYIDI
                     FKEASIQYLKEK"
BASE COUNT          465 a          278 c          229 g          551 t
ORIGIN      
        1 atgtatttga taatttacag ctcgttgagc tgctgttttc tagggattta tgtcccattc
       61 cttttacgag aaggttttta cgagttttat gtaactatta gaagctatct ttttatatga
      121 ccattagtca atatatgtat gacaaatcag cacaataatt tcagttttac atcacttaat
      181 caatgaaaat gtgcttgttg atatttgaat atttacattc aaacaatatt catcgctatt
      241 tctatcaact taaaattcgc aataaaaaac tgccagcacg cgaatagggc agctgacagc
      301 atcgttctta tttttctttt aaatattgaa tggaagcctc tttaaaaata tcaatatatt
      361 tcagatacat atctttttca atatattcat cgatttgatg tgccattaat ggattacctg
      421 gtccaaaaat ggctaaatca acattgtcct tattatctcc taagaaacta gaggcatctg
      481 ttgcacctac aagcgctgaa acaaatattt cgtcttgttc tacataacta gaagctacat
      541 ctttaatcgt agtaattaat ttgctatttt tatcgcttgt tacaggtcgg tgattgcttg
      601 gaatatcgag tgaaagctta ttgctatcca catcattaat gatattttgg aaaaacgatt
      661 ctataaagtc gttatcatac tcaggaactg gtcttacgtt aaattcaagt gaagcttcat
      721 ctggtacaga gttaaattgt ttgccgccat ttataatcga acatacagct gtaagaccag
      781 atgcataatt tgcatcctct tcagaaattt cttttccaat caatgatttg aacataggcg
      841 caacatctaa ttcatgttta gtatcttgtt gtttaagctc tgaatatttt tctttaaata
      901 gattataaaa ttcaagcagt gtatcaattg cattgtcacc aataaatgga actgagctat
      961 ggacagcttt accagttgca gttactttac atgacataga ccccttatgt gcataataaa
     1021 ttccagatcc agttggttca gcaataatta agccatcgac atcgtctaaa tagcctttat
     1081 cagctaataa tttggcacct tcttgttctt tctcttcgcc agcagtagcc agtaatctaa
     1141 tcgttccatg aggcaattca ttttgttctt ttaattcgat tagagatacg accaaagcca
     1201 ttaaaccgcc tttcatatct gtagtgcctc ggccatataa tttgccatct ttttctgtca
     1261 gttgaaaagg gggatatgac caattatctt gatttcctgc atcaacaaca tccatatgac
     1321 cactcaatgc gagtataggt gagccgttac cgatttctgc aacgatattg gcgcggtgtt
     1381 cattaacttt caaaatttca gatttaatat cgtacttgtc gaataaatct gttaaataat
     1441 tacaaacgtc tatttcatta ttattttcag tttgtagttc aacaatatct gctagtaatt
     1501 gaattttttc tttttcacta aaa
//
LOCUS       DAXWFK010000291         1518 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.295, whole genome shotgun sequence.
ACCESSION   DAXWFK010000291 DAXWFK010000000
VERSION     DAXWFK010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.295"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..1126
                     /gene="scpA"
                     /locus_tag="ACGEXS_002697"
     CDS             <1..1126
                     /gene="scpA"
                     /locus_tag="ACGEXS_002697"
                     /EC_number="3.4.22.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine protease staphopain A"
                     /protein_id="HFF8013644.1"
                     /translation="FSLSVTPIANAESNSNIKAKDKKHVQVNVEDKSVPTDVRNLAQK
                     DYLSYVTSLDKIYNKEKASYTLGEPFKIYKFNKKSDGNYYFPVLNTEGNIDYIVTISP
                     KITKYSSSSSKYTINVSPFLSKVLNQYKDQQITILTNSKGYYVVTQNHKAKLVLKTPR
                     LEDKKLKKTESIPTGNNVTQLKQKASVTMPTSQFKSNNYTYNEQYINKLENFKIRETQ
                     GNNGWCAGYTMSELLNATYNTNKYHAEAVMRFLHPNLQGQRFQFTGLTPREMIYFGQT
                     QGRSPQLLNRMTTYNEVDNLTKNNKGIAVLGSRVESRNGMHAGHAMAVVGNAKLDNGQ
                     EVIIIWNPWDNGFMTQDAKNNVIPVSNGDHYRWYSSIYGY"
     gene            1157..1483
                     /locus_tag="ACGEXS_002698"
     CDS             1157..1483
                     /locus_tag="ACGEXS_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphostatin A"
                     /protein_id="HFF8013645.1"
                     /translation="MEQIELFSIDKFKCNSEAKYYLNIIEGEWHPQDLNDSPLKFILS
                     TSDDSDYICKYINTEHKQLTLYNKNNSSIVIEIFIPNDNKILLTIMNTEALGTSPRMT
                     FIKHKS"
BASE COUNT          596 a          223 c          231 g          468 t
ORIGIN      
        1 attttcttta agtgttaccc ctattgcaaa cgctgagagc aattcaaata tcaaagcaaa
       61 agataagaag catgtgcaag ttaatgtcga ggacaagagt gttcctactg acgtacgtaa
      121 tttagcgcaa aaagactatc tttcttatgt tacttcactt gataaaatct ataataaaga
      181 gaaagcctcg tatacacttg gtgaaccttt taaaatttat aaattcaaca aaaagagtga
      241 tggtaattat tattttccag ttttgaacac tgaaggtaat atcgattata tagttacaat
      301 ttcaccaaaa atcactaagt attctagttc atcttctaaa tacacaatca atgtttctcc
      361 atttttatct aaagtcttaa atcaatataa agatcaacaa attactattt tgacaaactc
      421 aaaaggttac tatgtggtta ctcaaaatca taaagctaaa ctagtactaa aaacaccacg
      481 tctagaagat aaaaagttaa aaaagactga gagtatacct actggcaata atgtaacgca
      541 actaaagcaa aaagcttctg tcactatgcc aactagtcaa tttaaaagta ataattatac
      601 ttacaacgag caatatataa ataaacttga gaatttcaag ataagagaaa ctcaaggtaa
      661 caatggttgg tgtgcaggct atacgatgtc tgaattacta aatgcgactt ataatactaa
      721 caaatatcat gcagaagctg tgatgagatt tttacaccca aacttacaag gacaacgatt
      781 ccaatttact ggtttaacgc caagagagat gatttatttt ggacaaactc agggaagaag
      841 tccacaactt cttaatagaa tgactacata taatgaagtt gacaatctta ctaaaaataa
      901 taaaggaatt gctgttttag gtagtagagt agaatcaaga aatggtatgc acgcagggca
      961 tgccatggca gttgttggga acgctaaatt agataatgga caagaggtta taattatatg
     1021 gaatccatgg gataatggtt ttatgactca agatgctaaa aacaatgtta ttccagtatc
     1081 caatggcgac cattatcgat ggtattcatc tatttatggt tattaaaatt tgtattttag
     1141 aaaggtaaat aatattatgg aacaaattga attatttagt attgataaat tcaaatgtaa
     1201 ttcagaggct aagtattatc ttaatattat tgagggagaa tggcatcctc aagatttaaa
     1261 tgatagtcct ttaaaattta ttctcagtac ctcagacgat tctgattaca tttgcaaata
     1321 tataaataca gaacacaaac aactcacatt atataataaa aataatagct caattgttat
     1381 tgaaatattt ataccaaatg ataataaaat actactaaca attatgaaca cagaagcttt
     1441 gggaacttct cctagaatga ctttcattaa gcataagtca taagactatc tagatatgat
     1501 taagtaaact attaatct
//
LOCUS       DAXWFK010000292         1502 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.296, whole genome shotgun sequence.
ACCESSION   DAXWFK010000292 DAXWFK010000000
VERSION     DAXWFK010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.296"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            113..529
                     /locus_tag="ACGEXS_002699"
     CDS             113..529
                     /locus_tag="ACGEXS_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFF8013646.1"
                     /translation="MKTKEVVALMNISQDTLRYYEKVGVIPPVNRDENGYRIYNDSDL
                     NWIYLVENLRNAGVSIESLIEFCRLAQLPKNENIQAQQKQILNKQLEELNENLKTIHD
                     ARDLLQYKIDNYDNHIAKINASDNYDDNVERLWERK"
     gene            599..1447
                     /locus_tag="ACGEXS_002700"
     CDS             599..1447
                     /locus_tag="ACGEXS_002700"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFF8013647.1"
                     /translation="MNHIEISKDVKIPVLGFGVFQIPQEQTAEAVKEAIKAGYRHIDT
                     AQSYLNETEVGQGIEASGIDRSELFITTKVWIENVNYEDTIKSIERSLQRLNLDYLDL
                     VLIHQPYNDVYGSWRALEELKENGKIKAIGVSNFGVDRIVDLGIHNQIQPQVNQIEIN
                     PFHQQEEQVAALQQENVVVEAWAPFAEGKNQLFQNQLLQAIADKYNKSIAQVILRWLV
                     ERDIVVLAKSVNPERMAQNLDIFDFELTEEDKQQIATLEESNSQFFSHADPEMIKALT
                     SRELDV"
BASE COUNT          598 a          210 c          271 g          423 t
ORIGIN      
        1 aaagagacaa atttaaattt gagtaataaa ataagaagta ttcaaacgaa gatgacaata
       61 tataaagcaa cattacttta gacgaccaaa cataactgaa aggatagtcg caatgaaaac
      121 taaagaagtc gtagcgctca tgaatatatc tcaagacact ttaagatatt atgaaaaggt
      181 tggtgtgatt ccaccagtta atcgagatga aaatggatat agaatatata atgatagtga
      241 tttaaattgg atttatttag tggaaaattt gcgaaatgca ggcgtcagta ttgaatcgct
      301 tatcgaattt tgcaggttag cgcagcttcc taaaaatgaa aatattcaag cacagcaaaa
      361 gcaaatttta aataagcaac tcgaagaatt aaatgaaaac ttaaagacaa ttcatgatgc
      421 gagagattta ctacaatata aaattgataa ttatgataat catattgcta aaatcaatgc
      481 tagtgataat tatgatgaca atgttgaacg tctttgggag agaaagtaaa cctatggctt
      541 gctatagagt tcgctccatg gtttatagtg gacatacaag ataaaggagg ttcattttat
      601 gaatcatatt gaaataagta aagatgtaaa gataccagta ttaggtttcg gagtatttca
      661 aattcctcaa gaacaaacag cagaagcggt taaagaagca attaaagcgg gttatagaca
      721 tattgataca gcacaaagtt atttaaatga aactgaagta ggtcaaggta ttgaagcttc
      781 aggtattgat agaagtgaac tattcattac gactaaagta tggattgaaa atgttaacta
      841 tgaagatact atcaaatcaa tcgaacgatc attacaacga ttaaatttgg actatcttga
      901 tctagtgctt attcaccaac cttacaatga cgtatatggc tcatggcgtg cattagaaga
      961 gttaaaagaa aatggtaaaa ttaaagcaat cggtgtatct aacttcggcg ttgatcgaat
     1021 tgtagattta ggtattcaca atcaaattca accacaagtg aaccaaatag aaattaatcc
     1081 attccatcaa caagaagaac aagttgcagc acttcaacaa gaaaatgttg tggtagaagc
     1141 ttgggcacca tttgcagaag gtaaaaatca attattccaa aatcaattgt tgcaggccat
     1201 tgcagataaa tacaacaaat caattgcaca agttatttta cgctggttag tagaacgtga
     1261 cattgttgta ctcgcaaaat cagtgaatcc agaacgtatg gctcaaaact tagatatatt
     1321 tgattttgaa cttacagaag aagataaaca acaaattgcg acattagaag aaagtaatag
     1381 ccaatttttc tcacatgctg atccagagat gattaaagca cttacaagca gagaattaga
     1441 tgtttaatca taatataaca aagtaaatca tttacattac actaacctga aacttggatg
     1501 tt
//
LOCUS       DAXWFK010000293         1501 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.297, whole genome shotgun sequence.
ACCESSION   DAXWFK010000293 DAXWFK010000000
VERSION     DAXWFK010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.297"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..436
                     /gene="fmhC"
                     /locus_tag="ACGEXS_002701"
     CDS             <1..436
                     /gene="fmhC"
                     /locus_tag="ACGEXS_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhC"
                     /protein_id="HFF8013648.1"
                     /translation="ENPNSKKNKSKLNQLNMQLSSINNRISKTEELIFEDGPVLDLAA
                     ALFICTDDEVYYLSSGSNPKYNQYMGAYHLQWHMIKYAKSHNINRYNFYGITGVFSNE
                     ADDFGVQQFKKGFNAHVEELIGDFIKPVRPILYKFAKLIYKV"
     gene            609..1481
                     /gene="dprA"
                     /locus_tag="ACGEXS_002702"
     CDS             609..1481
                     /gene="dprA"
                     /locus_tag="ACGEXS_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFF8013649.1"
                     /translation="MIKLFLLKLYWAHFSTKQIHQFLMAYPNVIKEEGRKKDSYLCEW
                     VNREENVHLLRKYYAFIKLDHNDIIKELQKLKVSYITYMDSEYPVLLKEIYQFPLRLF
                     YKGNIKLINNMHHLAVVGARDSTSYTQQSLEFLLSNDKSKYLTIVSGLAQGADAMAHQ
                     IALKYNLPTIAVLAFGHQTHYPKSTLALRNKIEEKGLVISEYPPHTPIAKYRFPERNR
                     IISGLSKGVLITEAKEQSGSHITIDFALEQNRNVYVLPGSMFNPMTKGNLLRIQEGAK
                     VVLNANDIFEDYYI"
BASE COUNT          579 a          192 c          230 g          500 t
ORIGIN      
        1 tgaaaatcca aattctaaaa aaaataaatc aaaattaaat cagttaaata tgcaattatc
       61 tagtattaat aatagaatta gtaaaaccga agaactaata tttgaagatg gacctgtttt
      121 ggatttagct gctgctttat ttatatgtac tgatgatgaa gtttattatc tatcaagtgg
      181 atcaaatccg aaatataatc agtatatggg tgcatatcat ctacaatggc atatgataaa
      241 atatgcaaaa tcacataata ttaataggta taatttttat ggaataacag gcgtctttag
      301 taatgaggcg gatgattttg gtgttcaaca atttaaaaag ggttttaatg cacatgttga
      361 agaattaatt ggtgatttca tcaaaccagt aagaccaatt ctatataaat ttgcaaaact
      421 tatttataag gtttaattat aaagtatgtt ggaaattgaa attttaaatt ctttccaaca
      481 tacttttcac tttttaagtc agtattttta ctccttaaaa taacattaca tcgacaatgt
      541 ttttgtatta gaaatgtaaa acaaaaatag aatatgattt tgtatgatta caaaaaggag
      601 ttgatttatt gattaaacta tttttgctta agttatactg ggcacacttt tcgactaaac
      661 aaattcatca atttttaatg gcatatccta atgtaattaa agaggaggga agaaaaaaag
      721 atagttattt atgtgaatgg gtgaataggg aagaaaatgt tcatttatta cgtaaatact
      781 atgcttttat aaaacttgat cataacgata ttattaaaga actgcagaaa ttaaaagtaa
      841 gttacattac atatatggat tctgaatacc cagtgctatt aaaagaaata tatcaatttc
      901 cattacgtct tttctataaa gggaacatca aattaataaa taatatgcat catttggcag
      961 tagtaggtgc aagagattct acaagttata cccaacagtc tttagaattt ttattatcaa
     1021 atgataaaag caaatattta acaattgttt ccggccttgc tcaaggagct gatgcaatgg
     1081 cacatcaaat agctttaaaa tacaatctcc ctacaattgc agttttagcc tttggccatc
     1141 aaacacatta tcccaaaagt acattagcat taagaaataa aatagaagaa aaaggtttag
     1201 ttatatctga atatccacca catacaccaa tcgctaaata tagatttcct gagcgcaata
     1261 gaattatcag cggtttgtca aaaggggttt taattactga ggctaaggaa caaagtggca
     1321 gtcacatcac gatagatttt gcattagagc aaaatagaaa tgtttatgtt ttacctggat
     1381 ctatgtttaa tcctatgaca aaaggtaatt tattacgtat ccaagaaggt gctaaggtag
     1441 tattaaacgc taatgatata tttgaagact actatattta aaactaaaaa taacgagttg
     1501 c
//
LOCUS       DAXWFK010000294         1489 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.298, whole genome shotgun sequence.
ACCESSION   DAXWFK010000294 DAXWFK010000000
VERSION     DAXWFK010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.298"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            2..961
                     /locus_tag="ACGEXS_002703"
     CDS             2..961
                     /locus_tag="ACGEXS_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8013650.1"
                     /translation="MNRNIVKLVVFMLILVVAVAGCGQKDTEEKTEMTTIKDELGTEK
                     IKKNPKRVVVLEYSFADYLAALDMKPVGIADDGSTKNITKSVRDKIGAYESVGSRPQP
                     NMEVISKLKPDLIIADVSRHKKIKSELSKIAPTIMLVSGTGDYNANIEAFKTVAKAVG
                     KEKEGEKRLEKHDKILAEIRKKIEQSTLKSAFAFGISRAGMFINNEDTFMGQFLIKMG
                     IQPEVTKDKTTHVGERKGGPYIYLNNEELANINPKVMFLATDGKTDKNRTKFIDPAVW
                     KSLKAVKDNKVYDVDRNKWLKSRGIIASESMAEDLEKIAEKAK"
     gene            complement(1007..1138)
                     /locus_tag="ACGEXS_002704"
     CDS             complement(1007..1138)
                     /locus_tag="ACGEXS_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAR1012 family small protein"
                     /protein_id="HFF8013651.1"
                     /translation="MRYVTMRQFIKRTVKTILVGYVIKFIRNKLSGKSSHPTDNKHN"
     gene            complement(1202..1417)
                     /locus_tag="ACGEXS_002705"
     CDS             complement(1202..1417)
                     /locus_tag="ACGEXS_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HFF8013652.1"
                     /translation="MKVNKAIYVIVALFLGGLGIHKFYADKFGQGLFHLLFFWTGIPT
                     IISIIHAIFVIFTKKADKDGYIIFPKN"
BASE COUNT          569 a          197 c          277 g          446 t
ORIGIN      
        1 tgtgaatagg aatatcgtta aactagttgt gttcatgcta attttagttg tagcagtagc
       61 gggttgtggt caaaaagata ctgaagagaa aactgaaatg acgacaataa aagatgaatt
      121 aggaactgaa aaaattaaga aaaatcctaa acgtgttgtt gtattagaat atagttttgc
      181 tgattattta gcagcattag atatgaaacc tgttggtatt gcagatgatg gcagcactaa
      241 aaatataaca aagtcagtaa gagataagat tggggcatat gaatcggttg gatctagacc
      301 gcaaccgaat atggaagtga taagtaaatt aaaaccggat ttgatcattg cagatgttag
      361 cagacataag aaaatcaaat cagaattgag caaaattgct ccgacaatca tgttagttag
      421 cggtacggga gattataatg caaatattga agcatttaaa acagtcgcta aagcagtagg
      481 caaagagaaa gaaggcgaga agcgtctgga aaagcatgat aaaatattag cggagattag
      541 aaagaaaatt gaacagagta cgttaaaatc tgcatttgca ttcggtatct caagagcagg
      601 tatgtttatt aataatgaag atacatttat gggacaattc ttaattaaaa tgggtattca
      661 acctgaagtc acaaaagaca aaactacgca tgttggtgaa cgcaagggtg gtccttatat
      721 atatttaaat aatgaagaac ttgccaatat caatccaaaa gttatgtttt tagccactga
      781 cggaaaaacg gacaaaaata gaacgaaatt cattgatcct gcagtttgga aatcattaaa
      841 agctgtgaaa gataacaaag tttatgacgt tgaccgaaat aagtggttga aatcaagggg
      901 gattatcgca agtgaaagta tggcagaaga tttagaaaaa attgcagaaa aagcaaaata
      961 aaaatacagc gctactcgta aatcatataa gagtggcgct gtatttttaa ttatgtttat
     1021 tatctgtcgg atgtgatgat ttacctgaaa gtttatttcg aataaattta attacataac
     1081 cgacaaggat tgttttaaca gttcttttaa tgaattggcg catcgttaca tacctcattt
     1141 ctctatatct tacgaactat atacccattc atatatgctt tttaaacgtc attgtcacaa
     1201 tttaattttt agggaatata atataaccat ctttatctgc ttttttagta aaaatgacaa
     1261 aaattgcatg tattattgag atgatggtag ggatacctgt ccagaaaaat aataagtgaa
     1321 aaagaccttg tccaaattta tcagcataaa atttatgaat acctaaacct ccaagaaata
     1381 atgcaacaat aacataaatg gctttattga ctttcatttg taaatcctcc ttaactataa
     1441 ttctacttaa attcgttgtg aaaaccaata tttctaactt tagaatttt
//
LOCUS       DAXWFK010000295         1486 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.299, whole genome shotgun sequence.
ACCESSION   DAXWFK010000295 DAXWFK010000000
VERSION     DAXWFK010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.299"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            59..253
                     /locus_tag="ACGEXS_002706"
     CDS             59..253
                     /locus_tag="ACGEXS_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFF8013653.1"
                     /translation="MADESKFEQAKGNVKETVGNVTDNKNLENEGKEDKASGKAKEFV
                     ENAKEKATDFIDKVKGNKGE"
     gene            complement(486..1337)
                     /locus_tag="ACGEXS_002707"
     CDS             complement(486..1337)
                     /locus_tag="ACGEXS_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFF8013654.1"
                     /translation="MQQFKWINILKGFAMGTSDLVPGVSGGTIALLLGIYNQFIASIS
                     GIFSRRFWPSFTFLIPIIIGMLLAMGSLSNLFNYLLSQHHIPTMFFFGGLIIGIVPYL
                     LKISNYKTSFTTKHYMMVIAGIAILIVITLMNNGDKHAGETLTLSTSLIIKYFIAGMC
                     ASSAMLLPGISGSFMLLVFGVYGTVMLAISEVVKLNFAGLPILLAVGFGVLAGFIISS
                     KIIQYFLTHHKLMTFALIIGFVVGSLFAVFPGLPTNIVMWFVSLVVFIIGFIVSLTLG
                     RITAENE"
BASE COUNT          600 a          223 c          230 g          433 t
ORIGIN      
        1 atttttcagt gggtaagtat tatatataac atttaattat tccgaggagg catttattat
       61 ggcagacgaa agtaaatttg aacaagcaaa aggtaatgtt aaagaaacag taggtaatgt
      121 tactgataat aaaaatttag aaaacgaagg taaagaagat aaagcttctg gtaaagcgaa
      181 agaattcgtt gaaaatgcaa aagaaaaagc aactgatttt attgataaag taaaaggtaa
      241 caaaggcgag taattttact ttttattcct aatacactgc acatgccact tagtcggtat
      301 aaccatcggc taggtggttt ttgtattaaa aaagtggata ccaaaattta ttaataatta
      361 ttttaatgtt agaaaaaaac taaataaaaa ctcgctaatg atatccaata atatgtatac
      421 aaaacgagac atatattgca tatgattaac gagatactga aaatatttta tcacccctaa
      481 aatgattatt cattttcagc ggtaattcga cctaaagtca aacttacaat aaaaccgatg
      541 ataaatacta ctaatgaaac gaaccacatc acgatattag ttggtaaacc tggaaatact
      601 gcaaagaggg agccaacaac aaaaccaatg attaatgcaa aagtcattag tttatgatgt
      661 gttaggaaat actggataat tttgcttgaa ataatgaatc cagcaagcac gccaaatccg
      721 actgcaagta atataggaag acctgcaaag ttaagtttaa caacttcaga tattgctagc
      781 atgaccgtac catagacgcc aaatactaat aacataaatg accctgaaat acctgggagt
      841 aacatagcac tagatgcaca catacctgca ataaaatatt taataataag actagttgat
      901 agagtaagtg tttctccagc atgtttatca ccattattca ttaatgtaat aacaattaag
      961 atagcgatac cagctataac catcatgtaa tgtttagttg taaatgacgt tttatagtta
     1021 gaaattttca ataaatatgg aacgatacca atgattaatc caccaaagaa aaacatagtt
     1081 ggaatatggt gttggcttaa taaataatta aaaagattac ttagtgatcc cattgccagt
     1141 aacattccaa ttataatggg gattaaaaat gtaaaacttg gccaaaaacg tcgtgagaat
     1201 atgccgctaa ttgaagcgat aaattgattg taaataccta acaataatgc gatagtccca
     1261 ccgctaacac caggtaccaa gtcactcgtt cccatagcaa aaccttttag aatattaatc
     1321 catttaaatt gttgcatgaa taactccttt caaacgattg gaataaaatc ataaatagca
     1381 tcataccata ttacaaatgt cctagtgaaa tgataacata ttttaaattc ataaaatcca
     1441 ttgagaaatt atgtgcactt attatcattt atatttttaa agagag
//
LOCUS       DAXWFK010000296         1481 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.300, whole genome shotgun sequence.
ACCESSION   DAXWFK010000296 DAXWFK010000000
VERSION     DAXWFK010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.300"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..428
                     /locus_tag="ACGEXS_002708"
     CDS             <1..428
                     /locus_tag="ACGEXS_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5080 family protein"
                     /protein_id="HFF8013655.1"
                     /translation="HDLSNFLMFMDTGSYIFMYFAIKCLWVKPKVVNYLIAKELGESK
                     EVIEEQELDLQTSKIRGIYFFIISVVMLIITKLRMQPELQADAISMNPVFIFVGVIII
                     LIWLVLDIYRKKKYGIFLFKTIVPLVVTTWIIIATIILS"
     gene            complement(571..1464)
                     /locus_tag="ACGEXS_002709"
     CDS             complement(571..1464)
                     /locus_tag="ACGEXS_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HFF8013656.1"
                     /translation="MKKTILLTMTTLTLFSMSPNSAQAYTNDSKTLEEAKKAHPNAQF
                     KVNKDTGAYTYTYDKNNTPNNNHQNQSRTNDNHQHANQRDLNNNQYHSSLSGQYTHIN
                     DAIDSHTPPQTSPSNPLTPAIPNVEDNDDELNNAFSKDNKGLITGIDLDELYDELQIA
                     EFNDKAKTADGKPLALGNGKIIDQPLITSKNNLYTAGQCTWYVFDKRAKDGHTISTFW
                     GDAKNWAGQASSNGFKVDRHPTRGSILQTVNGPFGHVAYVEKVNIDGSILISEMNWIG
                     EYIVSSRTISASEVSSYNYIH"
BASE COUNT          416 a          220 c          289 g          556 t
ORIGIN      
        1 atcatgattt aagcaacttt ttaatgttta tggatactgg atcatatatt tttatgtact
       61 ttgcaattaa gtgtttatgg gtgaaaccta aggtagtaaa ttatttgatt gcaaaagaat
      121 tgggtgaatc taaagaggtc attgaagagc aggaattaga tttacagaca tcaaagataa
      181 gaggtatcta ctttttcatt atttcagtag tgatgttaat tatcactaaa ttaagaatgc
      241 aacctgagtt acaggcagat gctatatcga tgaatcctgt atttattttt gttggtgtta
      301 taatcatttt aatttggcta gtactggata tatatcgcaa gaaaaaatac ggtatattct
      361 tattcaagac gatagtgcca ctagttgtta cgacttggat tattatagct acaattatac
      421 tttcgtaaac gattttgatt aatggggaat gtagagaaca tttctttgta attttataat
      481 cgaataacat acaaaataaa gcgaccaatt ttgcagtgtt acgaattgta aactgcgaac
      541 tggtcgcttt ttattgatgt catgattaat ttaatggatg taattatatg atgaaacttc
      601 tgaagcagag atggttcttg atgaaacgat atattcacca atccagttca tttctgaaat
      661 tagaatactt ccatcaatat taactttttc aacgtaggct acatgaccaa atggaccatt
      721 tactgtttgt aaaattgatc ctcgtgttgg gtgtctatct actttgaagc cattgcttga
      781 agcttggcct gcccagtttt tagcatctcc ccaaaatgta ctaatcgtgt gtccatcttt
      841 ggcacgttta tcaaagacat accatgtaca ttgtccagca gtatataagt tgttcttact
      901 tgtgataaga ggctgatcaa tgattttacc gttacctaat gctaaaggtt taccgtcagc
      961 ggtctttgct ttgtcattaa attcggcgat ttgtaattcg tcatacaatt cgtctaaatc
     1021 gatgcctgta ataagccctt tgttatcttt tgaaaaagcg ttatttaatt catcgtcatt
     1081 gtcttcgaca ttcggtattg ctggtgtcaa aggattgctt ggtgacgttt gaggcggtgt
     1141 gtgtgaatca attgcgtcat taatgtgcgt atactgacca cttaatgaag aatggtactg
     1201 attgttgtta agatcacgtt gatttgcgtg ttgatgattg tcgtttgtac gtgactggtt
     1261 ttgatgattg ttgtttggcg tgttgttttt gtcatatgta taagtatacg cgccggtgtc
     1321 tttattcact ttgaactgtg cgtttgggtg tgctttcttt gcttcttcta atgttttgct
     1381 atcattcgta tatgcttgag ccgagttagg cgacatacta aataaagtaa gagttgtcat
     1441 cgtcagtaaa attgttttct tcataataac catttaatcc t
//
LOCUS       DAXWFK010000297         1478 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.301, whole genome shotgun sequence.
ACCESSION   DAXWFK010000297 DAXWFK010000000
VERSION     DAXWFK010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.301"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            117..1460
                     /gene="brnQ"
                     /locus_tag="ACGEXS_002710"
     CDS             117..1460
                     /gene="brnQ"
                     /locus_tag="ACGEXS_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFF8013657.1"
                     /translation="MNKNTWVIGFTLFAMFFGAGNLIFPPNLGLDSGQFFWPAILAFV
                     LTGIGLPLLGVIVGALDKEGYIGALNKISPKFSILFLIIIYLTIGPLFAIPRTASTSF
                     EMTITPIIHSNSSIALFIFTIIYFIVVLYICLNPSKLIDRIGSLLTPLLLITILAMII
                     KGYLDFSGNSAGKGNEALYHSNFSSFAEGFTQGYLTMDAIAAIAFSMIVVNAVKLTGI
                     TKTNQIFKQTLTAGLIAAVALIFIYISLGYIGNHMPVSDMTLDQLKSKDRNIGTYLLT
                     TMASTGFGSFGKYLLGIIVALACLTTACGLIVAVSEYFHRIVPKVSYKAFVLVFILMS
                     FIIANQGLNAVISMSIPVLSIVYPVAITVVLLILIAKFIPTKRISQQIPVIIVFILSI
                     FSVISKLGWLKINFIESLPLRAYSLEWFPVAIIATILGYLVGIFVKQDPIKYQQE"
BASE COUNT          461 a          225 c          241 g          551 t
ORIGIN      
        1 acacatgtgt tttgtttctg ctatgtacaa catatttcat atagactctt tattattttt
       61 tgtgctaaaa tatttacttg taaattattt cgtgttttaa aaaaaggagt aactctatga
      121 ataaaaatac atgggtcatt gggtttacgc tcttcgctat gttttttggc gcaggcaatc
      181 taatttttcc tcctaatctc ggattggata gtggtcaatt tttctggccc gccattttag
      241 cgtttgttct aactgggatt ggtttaccat tattaggtgt gattgtaggt gcacttgata
      301 aagaaggata tattggcgca ttaaataaaa tttcacctaa attttcaata ttgttcttaa
      361 tcatcattta tttgactata ggaccacttt ttgcaatacc tagaactgca tctacatctt
      421 ttgaaatgac aattacacca attatacata gcaatagtag tatcgcttta tttatattta
      481 cgattatcta cttcatagtc gttttgtata tttgtttaaa tccatctaag ttaatcgatc
      541 gtattggttc attattaaca ccattattat tgattactat tttagcgatg attattaaag
      601 gatacttaga ctttagcggt aatagtgctg gaaagggcaa tgaagcacta tatcattcta
      661 atttttcaag ttttgctgaa ggctttacac aaggctattt aacaatggat gccattgcag
      721 caattgcttt ttcaatgatt gttgttaatg cagtaaaact aacaggcatt actaaaacaa
      781 atcaaatatt caaacaaact ttgactgctg gtttaattgc agccgtagct ttaattttca
      841 tatatatttc attaggttat attggtaatc atatgccagt aagtgacatg acgttagatc
      901 aattgaaatc caaagatcga aacattggga catatttatt aacgacaatg gcttcaacag
      961 gatttggttc attcggaaaa tatttattgg gcatcattgt ggcgctggca tgtctaacta
     1021 cagcatgcgg gcttattgtt gcagtttctg aatatttcca tagaatcgta cctaaagtat
     1081 catacaaagc atttgtatta gttttcattt taatgagttt tattattgct aaccaaggtt
     1141 taaatgctgt tatctcaatg tcaattccgg tattaagcat tgtataccca gtagcaataa
     1201 ctgttgtatt attaatttta attgccaaat tcataccgac aaaacgcatt tcacaacaaa
     1261 ttccagttat tatcgtattt atattgtcga ttttcagtgt tattagtaag ttaggttggc
     1321 tgaaaattaa ctttatagaa tcattgcctc taagagcgta ttctttagag tggttcccag
     1381 tagcaattat tgcaacgata ttaggctatc tagtcggcat atttgtaaaa caagatccaa
     1441 ttaaatatca acaggaataa cgaataatat aaaagagg
//
LOCUS       DAXWFK010000298         1476 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.302, whole genome shotgun sequence.
ACCESSION   DAXWFK010000298 DAXWFK010000000
VERSION     DAXWFK010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.302"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(31..270)
                     /locus_tag="ACGEXS_002711"
     CDS             complement(31..270)
                     /locus_tag="ACGEXS_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HFF8013658.1"
                     /translation="MSNSDLNIERINELAKKKKEVGLTQEEAKEQTALRKAYLESFRK
                     GFKQQIENTKVIDPEGNDVTPEKIKEIQQKRDNKN"
     gene            complement(406..639)
                     /gene="sosA"
                     /locus_tag="ACGEXS_002712"
     CDS             complement(406..639)
                     /gene="sosA"
                     /locus_tag="ACGEXS_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-induced cell division inhibitor SosA"
                     /protein_id="HFF8013659.1"
                     /translation="MFYNKYKNVSTYIIIFLVSSAAFAIFLLSANISAHSEQVYEMTD
                     HQIKNNTINKAYEHKDPTNNSEQRDGKVFALIN"
     gene            782..1405
                     /gene="lexA"
                     /locus_tag="ACGEXS_002713"
     CDS             782..1405
                     /gene="lexA"
                     /locus_tag="ACGEXS_002713"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFF8013660.1"
                     /translation="MRELTKRQSEIYNYIKQVVQTKGYPPSVREIGEAVGLASSSTVH
                     GHLSRLEEKGYIRRDPTKPRAIEIVSDQTNDNINMEETIHVPVIGKVTAGVPITAVEN
                     IEEYFPLPEHLTSTHNSDIFILNVVGDSMIEAGILDGDKVIVRSQTIAENGDIIVAMT
                     EEDEATVKRFYKEKNRYRLQPENSTMEPIYLDNVAVIGKVIGLYREM"
BASE COUNT          492 a          228 c          230 g          526 t
ORIGIN      
        1 atgtcagaaa attctttaca gatttgtgat ttaattttta ttatctcttt tttgttgtat
       61 ctctttaatt ttttcaggtg ttacatcatt accttctgga tcaattactt tagtattttc
      121 aatttgttgt ttaaaccctt ttctaaaact ctcaagataa gcttttctta aggctgtttg
      181 ctcctttgct tcttcttgag ttaatcctac ttcttttttc tttttagcta actcgttaat
      241 tctttcgata ttcaaatcag aattactcaa ttaaaaaacc taccttcaat ttgtttattc
      301 aatcattata atactttaac aaaatataac aaatttaaga tcagcaaaaa agtaataaaa
      361 aacgctattt cgcaaaaata ggcaaaataa cgttgtgaca atgtatcaat ttattaaagc
      421 gaacactttc ccatctcttt gttcgctatt gtttgtaggg tctttatgtt cgtatgcttt
      481 atttatcgta ttgttcttaa tttgatggtc agtcatttcg tacacttgtt ccgagtgagc
      541 actaatgttc gcacttaaca agaatattgc aaaggctgca cttgaaacta aaaatatgat
      601 gatatatgtt gatacgtttt tatatttatt gtaaaacatc attttcactc ctagaacatt
      661 tgtttgtatt agtaatttat cagaacttat gttctgtgtc aatattttaa cgaacaaatg
      721 tttgttttgg tttcaaatta atgatatact gtatttaaat taatttaggg agtgcctata
      781 tatgagagaa ttaacaaaac gacaaagcga aatatataac tatattaaac aagttgttca
      841 aacgaaaggt tatccgccta gtgttcgcga aattggtgaa gcagttggct tagcatccag
      901 ttcaactgtt catggtcacc tttcacgtct tgaagaaaaa ggctatataa gaagagatcc
      961 aacgaaacca cgtgctatag aaattgtaag tgatcaaaca aatgataata ttaatatgga
     1021 agaaacgatt catgtgccag ttattggtaa agtcacagca ggtgttccta ttaccgcagt
     1081 agaaaatatt gaagaatatt ttccattacc tgaacactta acatcgacac acaatagcga
     1141 catattcata ttaaacgtcg taggcgacag tatgattgag gctggtatat tagacggaga
     1201 caaagtaatt gttcgcagtc aaaccatagc agaaaatgga gacattattg ttgctatgac
     1261 tgaggaagat gaagcaactg tcaaacgctt ctataaagaa aaaaatcgtt atcgattaca
     1321 acctgaaaat agtacaatgg agccaattta cctagacaat gttgctgtaa ttgggaaagt
     1381 aattggtttg taccgcgaaa tgtaatattt taaaccgtta tatattatcg taattgttaa
     1441 gccctcattt ttataaattt tggacctctt gaaaaa
//
LOCUS       DAXWFK010000299         1454 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.303, whole genome shotgun sequence.
ACCESSION   DAXWFK010000299 DAXWFK010000000
VERSION     DAXWFK010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.303"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            462..563
                     /locus_tag="ACGEXS_002714"
     CDS             462..563
                     /locus_tag="ACGEXS_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013661.1"
                     /translation="MKFAANKLTLTIFRKFCINNEKEIWIKYEEVFL"
     gene            585..845
                     /locus_tag="ACGEXS_002715"
     CDS             585..845
                     /locus_tag="ACGEXS_002715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HFF8013662.1"
                     /translation="MLYLTLALGLSTAAYASTEYAEGGTWSHGVGSKYVWSYYYHGHK
                     GHGATAIGKYRSFSGYTRAGVKAKASATKHNWWVNRAYYNIY"
     gene            889..>1454
                     /locus_tag="ACGEXS_002716"
     CDS             889..>1454
                     /locus_tag="ACGEXS_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HFF8013663.1"
                     /translation="MKWFKLILDVTIFILIAILLFVYTYKENEEILPDTKYPIAVTDW
                     NKKYSKNEIYKRIDQFAKNENVAIYKSTSNYTNKNVDKDIYVFNKSKAATITPFNAKY
                     NIHYLSDDEVLKKDIKGSYFVKDKNFDVSKFINFLKEYGVTAESYKIDHMMIAVGVIK
                     QMNIVVPLSSLLIVYFIYYIFEKNINFKA"
BASE COUNT          559 a          186 c          219 g          490 t
ORIGIN      
        1 taggcgacat gttcctgcat gtcgtctttt tacattttta tagtaacata tcttttattt
       61 aattgtctat ttttattaga tgtgtaacaa aattttgaag tattttccgt atattgcctg
      121 ctaaacatgt cagatttgaa aggaaaatca tactgtggta gaaatcaatt atgattatat
      181 ggtagtactt ataaataagt gatttctatt gaatatgtaa tgaagttcat atattaatat
      241 aactttcatt gatatagaag tcgcaatgat taatttaaac aataaaaaac aaccatcatt
      301 tccaaacttt gacaacgaat gatggttaaa gaaaatctca tttagtcacc gtctttttaa
      361 cgggctcaat aggcgacatg ttcctgcata tcgtctattt atacactgca caatttaata
      421 ataatacaat tatactttga tgtccattta acataaagtg gttgaaattt gcagcaaata
      481 aattaacctt gactattttc agaaaattct gtataaataa cgaaaaagag atatggatta
      541 aatatgaaga ggtgttttta tagaagaata aaatttttgg cactatatta tatttaactt
      601 tagcacttgg attatcaaca gcagcttatg catctacaga atacgcagaa ggaggcactt
      661 ggagtcatgg tgtcggcagt aagtatgttt ggtcgtatta ttatcatggt cataaaggac
      721 atggtgcaac agctattgga aaatatagat catttagtgg ttatacaaga gctggtgtaa
      781 aagcaaaagc atcagctact aaacataatt ggtgggtcaa tagagcgtat tataacattt
      841 attaaatact aaaaaatcat agaaaataaa caacgcaaga ggtacaacat gaaatggttc
      901 aaattaatac ttgatgttac aatcttcatt ctgattgcta tattgttatt tgtttataca
      961 tataaagaaa acgaagaaat attgcctgat actaaatacc ctatagcggt aactgactgg
     1021 aataaaaaat atagtaaaaa tgagatttat aaacgtatag atcaattcgc taaaaatgag
     1081 aacgtagcaa tctataaatc aacttcaaat tatacaaaca aaaacgtaga taaagatata
     1141 tatgtattta ataaatcaaa agcagcaact atcactcctt ttaatgctaa atataacatt
     1201 cattatttaa gtgacgacga agtattaaaa aaagatatca aagggagtta ttttgtaaaa
     1261 gacaaaaatt ttgacgtgtc taaattcata aattttttaa aagaatatgg tgttactgct
     1321 gaatcataca aaatagatca tatgatgatt gccgttggtg tcattaaaca aatgaatata
     1381 gtagttccat tatcctcact tttaatcgtt tattttattt attatatttt cgaaaagaac
     1441 attaatttca aagc
//
LOCUS       DAXWFK010000300         1453 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.304, whole genome shotgun sequence.
ACCESSION   DAXWFK010000300 DAXWFK010000000
VERSION     DAXWFK010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.304"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..444
                     /gene="splD"
                     /locus_tag="ACGEXS_002717"
     CDS             <1..444
                     /gene="splD"
                     /locus_tag="ACGEXS_002717"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplD"
                     /protein_id="HFF8013664.1"
                     /translation="FYNNGGGLYKVTKIVDYPGKEDIAVVQVEEKSTQPKGRKFKDFT
                     SKFNIASEAKENEPISVIGYPNPNGNKLQMYESTGKVLSVNGNIVTSDAVVQPGSSGS
                     PILNSKREAIGVMYASDKPTGESTRSFAVYFSPEIKKFIADNLDK"
     gene            602..1318
                     /gene="splE"
                     /locus_tag="ACGEXS_002718"
     CDS             602..1318
                     /gene="splE"
                     /locus_tag="ACGEXS_002718"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplE"
                     /protein_id="HFF8013665.1"
                     /translation="MNKNIIIKSIAALTILTSVTGVGTTVVEGIQQTAKAEHNVKLIK
                     NTNVAPYNGVVSIGSGTGFIVGKNTIVTNKHVVAGMEIGAHIIAHPNGEYNNGGFYKV
                     KKIVRYSGQEDIAILHVEDKAVHPKNRNFKDYTGILKIASEAKENERISIVGYPEPYI
                     NKFQMYESTGKVLSVKGNMIITDAFVEPGNSGSAVFNSKYEVVGVHFGGNGPGNKSTK
                     GYGVYFSPEIKKFIADNTDK"
BASE COUNT          574 a          214 c          241 g          424 t
ORIGIN      
        1 ttttataata acggtggtgg actttataaa gttactaaga ttgtagatta tcctggtaaa
       61 gaagatattg cggttgtaca agttgaagaa aaatcaacac aaccaaaagg tagaaaattc
      121 aaagatttca ctagcaaatt taatatagca tcagaagcta aagaaaatga acctatatca
      181 gtcattggtt atccaaatcc taatggaaat aaactacaaa tgtatgaatc aactggtaaa
      241 gtactatcag tgaatggaaa tatagtgaca tctgatgcgg ttgtccaacc tggcagctct
      301 ggttcaccta tattaaatag taagcgagaa gcaattggtg ttatgtatgc tagtgataaa
      361 ccaacaggtg aaagtacaag gtcatttgct gtttatttct ctcctgaaat taagaaattt
      421 attgcagata atttagataa ataaatcatc catccataca ttgataaatg atttttagaa
      481 attaacaaca aaatcaacaa ttttaaacat ctctgtgatt ctatttattc gaaatgattt
      541 aaaaaataaa acttcaaaaa cctaacctta tatttatacg aatacttaga ggagcacaaa
      601 aatgaataaa aatataatca tcaaaagtat tgcagcattg acgattttaa catcagtgac
      661 tggcgtcggc acaacagtgg ttgagggtat tcaacaaacg gctaaagctg aacataatgt
      721 gaaactaatc aaaaatacta atgtagcacc atacaatggt gtcgtttcga taggatctgg
      781 aacaggtttc attgtcggta aaaatacaat tgttaccaac aagcatgtcg ttgcaggtat
      841 ggaaattggt gcacatatta tagcgcatcc caatggtgaa tataataatg gcggatttta
      901 taaagttaaa aaaattgtcc gttattcagg tcaagaagat attgccattc tacatgtgga
      961 agataaagct gttcatccaa aaaacaggaa ttttaaagat tacacaggca ttttaaaaat
     1021 agcatcagaa gctaaagaaa atgaacgcat ttcaattgtt ggctatccag aaccatatat
     1081 aaataaattt caaatgtatg agtcaacagg aaaagtgctg tcagttaaag gcaacatgat
     1141 tattactgat gctttcgtag aaccaggcaa ctcaggttca gctgtattta acagtaaata
     1201 cgaagttgta ggtgttcact ttggtggaaa cggccctgga aataaaagta caaaaggata
     1261 tggtgtttat ttctctcctg aaattaagaa attcattgca gataacacag ataaataaat
     1321 ccttacatag ataaatgatt ttaaaaatta acaacaaact caacaattca aatcatctct
     1381 gtgattccat ttattcgaaa tgattaaaaa aaataaaact tcaaaaagct aacattataa
     1441 ttatacaaat act
//
LOCUS       DAXWFK010000301         1447 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.305, whole genome shotgun sequence.
ACCESSION   DAXWFK010000301 DAXWFK010000000
VERSION     DAXWFK010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.305"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            295..753
                     /locus_tag="ACGEXS_002719"
     CDS             295..753
                     /locus_tag="ACGEXS_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFF8013666.1"
                     /translation="MKREVILKLQQFIIERENADKRRQYKKENEELDLSLTQFHIIEL
                     IDNNDKVNNKFLSEMLNVSKPAITKSIKKLLAKDLVVESHNEFNKREVNYSLTQKGKK
                     LSYIHDELHEKSVKKYEEVLKVFDDDEMAVIIEFLNRSIEELKKEDGLND"
     gene            746..>1447
                     /locus_tag="ACGEXS_002720"
     CDS             746..>1447
                     /locus_tag="ACGEXS_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8013667.1"
                     /translation="MINSNIRLYILGFLAFFASLIQNIYTPIIPRLYDDFQVPLFWIN
                     ATVGGFIFIVAVMQIVLGRSIDSRDSKKVLLTGLGIVIISSFICAVTHNFILFAISRL
                     FQAIGCGIIPLVTLTLLAKLSTDNGRAQAMANYQIFLSCAPALAPILGSTLGARWDYI
                     GIFSFLLVISIVLFLIIFFIDIPNVEKGIVKLTEKIEEKYLTDKVFITLVTLGFLIFF
                     TYFSILVYLPTLLNNT"
BASE COUNT          540 a          156 c          250 g          501 t
ORIGIN      
        1 ttttgcagca tgcaaatggc attgctacat ttattaaaat gcgcaatttg ttatgagtaa
       61 cttttttgga tagtaggcac attaaagtgt cgaaaacaat ttgtgtggaa gacagggtaa
      121 attctgctta tttaaaatga tagtttgtat tataggtgga tttgtgaaat gcggaataaa
      181 tggtgcataa ctaagtaaat atatgttaat tacaacaata tttgacaaaa atgggagtga
      241 caacaataat atagttaacc ggttaactaa tgaagaggag gaataatagt ggatatgaag
      301 agagaagtta ttttaaaatt acaacaattt attatagaaa gagaaaatgc cgataaaaga
      361 agacagtata aaaaggaaaa tgaggaacta gatttatctc taacacaatt tcatatcata
      421 gagttgattg ataataatga taaagtgaat aataaatttt tgtctgaaat gttaaatgta
      481 tcaaaaccag caattactaa atcgataaaa aaacttttag cgaaagactt agtagttgaa
      541 tcgcacaatg aatttaacaa aagagaagtt aattattcat taacacaaaa aggaaaaaaa
      601 ttatcttata tacatgatga attacatgaa aaatcggtaa aaaaatatga agaggtactt
      661 aaagtctttg atgatgatga aatggcagtt attatagagt ttttaaaccg tagtatagaa
      721 gaattaaaaa aagaagatgg tttaaatgat taatagtaat atcagattat atattctagg
      781 atttttagca tttttcgcat cattgattca aaacatatac acgcctatta ttccgagact
      841 atatgacgat tttcaagtac cactattttg gataaatgcg acagttggcg gttttatatt
      901 tatcgttgca gttatgcaaa ttgtacttgg tagaagtata gattctcgtg attcgaaaaa
      961 agtactttta acgggattgg gaatagtaat catatctagc tttatttgtg cagtgacaca
     1021 taactttata ttatttgcta tatcaaggtt atttcaagca attggttgcg gaattattcc
     1081 tctcgtgact ttaactttat tagccaaact aagtacagat aatggtagag ctcaagctat
     1141 ggcgaactat caaatatttt tatcatgtgc accagctttg gcaccaatct tagggagtac
     1201 tttaggtgca agatgggatt atattggtat ttttagtttt ttgctcgtta tatctatcgt
     1261 attatttttg attatctttt ttattgatat tccaaatgtt gaaaaaggga ttgtaaaatt
     1321 aactgaaaaa attgaagaaa aatatttgac cgataaagtg tttattactt tagtgacatt
     1381 gggattttta atatttttta cgtacttttc aatattagtt tatttaccaa cattattaaa
     1441 taatacc
//
LOCUS       DAXWFK010000302         1435 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.306, whole genome shotgun sequence.
ACCESSION   DAXWFK010000302 DAXWFK010000000
VERSION     DAXWFK010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.306"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            44..580
                     /gene="amaP"
                     /locus_tag="ACGEXS_002721"
     CDS             44..580
                     /gene="amaP"
                     /locus_tag="ACGEXS_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFF8013668.1"
                     /translation="MKRLKNFILGLLIVVIVGFLLFMYIQDGRITEYQDYFLQFEWFQ
                     PLLISLAALLILIGLILVFSIFKPTHRKPGLYKDFDDGHVYVSRKAVEKTAFDTVAKY
                     DQVRQPNVVAKLYNKKNKSYIDIKTDFFVPNNVQVQSLTEAIRSDIKKNVEYFTEMPV
                     RKLEVNVRDQKTSGPRVL"
     gene            593..832
                     /locus_tag="ACGEXS_002722"
     CDS             593..832
                     /locus_tag="ACGEXS_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFF8013669.1"
                     /translation="MANNHNQNGQDSTQQVINFLKVFKWRIVGFLAFLLIAILFLTLG
                     FWKTVLIIVLCLIGVGIGYMKDRKQDFMNFLNRWS"
     gene            895..1404
                     /locus_tag="ACGEXS_002723"
     CDS             895..1404
                     /locus_tag="ACGEXS_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFF8013670.1"
                     /translation="MTVDNNKAKQAYDNQTGVNEKEREERQKQQEQNQEPQFKNKLTF
                     SDEVVEKIAGIAAREVKGILDMKGGLTDTFTNAFSSGNNVTQGVSVEVGEKQAAVDLK
                     VILEYGESAPKIFRKVTELVKEQVKYITGLDVVEVNMQVDDVMTQKEWKQKHEKNNEN
                     NNQERQGLQ"
BASE COUNT          551 a          198 c          257 g          429 t
ORIGIN      
        1 atatgttttt aaattaactt taactaagga ggtgtcgtta ggggtgaaac gacttaaaaa
       61 ctttatcctt ggcttattaa tagtagtaat cgtggggttc ctgttattca tgtatatcca
      121 agatggacgt ataactgaat atcaagacta cttccttcaa tttgaatggt tccaaccatt
      181 acttatttca cttgctgcac tattgatatt aataggtctt atattagtat ttagtatctt
      241 caaacctacg catcgaaaac ctggattata caaagacttc gatgatggtc atgtttacgt
      301 atctcgtaaa gctgttgaaa agacggcatt cgatacggtt gcaaaatatg atcaagtaag
      361 acaaccaaat gtagttgcga aactatataa caaaaaaaat aaatcttata tcgacattaa
      421 aactgacttt ttcgtaccaa ataatgttca agttcaatct ttaactgaag caattcgttc
      481 ggatattaaa aagaatgtag aatatttcac agaaatgcca gtacgtaagt tagaagttaa
      541 tgtgagagat cagaaaactt ctggtccacg agtgttgtaa gggaggtata tcatggctaa
      601 caatcataac caaaacggac aagactctac acaacaagtg attaattttt taaaagtatt
      661 taaatggaga atcgttggtt tcctagcgtt tctattaatc gcgatattat tcttaacgtt
      721 aggtttttgg aaaacagtac ttatcatcgt tttatgttta attggtgtag gtattgggta
      781 tatgaaagac cgtaagcaag attttatgaa ttttttaaat cgatggagtt aattcatcaa
      841 tttagaattt aattaaaaaa atataaaaat taacaagtaa aaggagaatc tattatgact
      901 gtagataaca ataaagcaaa acaagcatac gacaatcaaa ctggtgttaa cgaaaaagaa
      961 agagaagagc gtcaaaaaca acaagaacaa aatcaggagc ctcaattcaa aaacaaatta
     1021 acattctctg atgaagttgt tgaaaaaatt gctggtatcg ctgcacgtga agttaaaggt
     1081 atcttagata tgaaaggtgg cttaactgat acattcacta atgcattctc aagtggcaac
     1141 aatgttactc aaggtgtatc tgttgaagtt ggtgaaaaac aagctgctgt agacttaaaa
     1201 gtaatcttag aatatggtga atcagcacca aaaatcttcc gtaaagtaac tgaattagtt
     1261 aaagaacaag ttaaatatat tactggttta gatgtagttg aagttaacat gcaagttgac
     1321 gatgtaatga ctcaaaaaga gtggaaacaa aaacacgaaa aaaataacga aaacaataac
     1381 caagaaagac aaggtttaca ataagttatt cgataaaaag ggcttgggac attaa
//
LOCUS       DAXWFK010000303         1418 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.308, whole genome shotgun sequence.
ACCESSION   DAXWFK010000303 DAXWFK010000000
VERSION     DAXWFK010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.308"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(444..1373)
                     /locus_tag="ACGEXS_002724"
     CDS             complement(444..1373)
                     /locus_tag="ACGEXS_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFF8013671.1"
                     /translation="MLLFYIIGVLVGMLIPIQTSVNSRLSLYTKSPFYTSFISFSVGT
                     ICLIILNIIINPEVFTIHFYNNQSFNYTWVVGGLLGVSFLTGNLLLLPKLGATLTVIA
                     TVAGQIIMGVIIDTFGLFGATIHDFNLIKAIGVLLLIVGIVIMNQFNKNNLLLTDQKY
                     LLFWLLLGFIFGFFPPIQTTINSALASHTHSPAFASLVSFTIGSIALLILTAIFNRSL
                     KLKTSHLKFGKLKPIYFTGGILGMAFVTANIILMPHMGAALTTLIGMFGQILMGILID
                     HFGLFGSPKIAMTSRKTIGLLCILTGIILLRLF"
BASE COUNT          568 a          200 c          205 g          445 t
ORIGIN      
        1 taagaaaaac acattaattt agtgattaaa accaatctat agcacgttaa agtgtctata
       61 cgttattccg attgataacg ggtaacaaat aatagaaagt acgataattg atcatgcatt
      121 aaagcacttt tatttttcaa actcgactca atgaattata aaaagttgtg cattttgact
      181 tatttttaat taattgaata accaactatg aaatattagc acgaataaca taatttttgc
      241 tattaatttg gtgtaaatta ttaaaaaata acattaattt aggtcttaag aatgagttga
      301 ctatcgaatt tgctaaatta aaatttgttg tgtcaaagca tactacccaa atcaacgctt
      361 ttctaaagat ttttagacca caaaaaaata gcttatcact tttaaaaata attacaagtt
      421 aaaatgataa gctaaaagtt aatttaaaat aatcttaaaa gtataatgcc tgtcaaaata
      481 cataatagac caatagtttt tctggatgtc attgctattt taggtgaacc aaataatcca
      541 aagtgatcta tcaatatgcc cattagaatc tggccaaaca tcccaataag tgttgttaat
      601 gctgcaccca tatgaggcat taagataatg ttagctgtta caaaagccat gccaagtata
      661 ccgccagtaa aatagatagg ctttaattta ccgaatttta aatgacttgt ttttagtttt
      721 aaagaacgat taaaaatagc ggttaaaatc aatagcgcta ttgacccaat tgtaaatgat
      781 actaatgatg caaaggctgg tgaatgagta tgactagcta aagcactatt aattgtcgtt
      841 tgaataggtg gaaagaaacc aaaaataaat cctaatagaa gccaaaacag taaatacttt
      901 tgatcagtta gtaataaatt attcttgtta aattgattca ttatgacgat gccgacaatg
      961 agtaacaata ctccaattgc tttaattaaa ttaaaatcat gaattgtagc gccaaataat
     1021 ccaaatgtat caataatgac acccataata atttgacccg caactgttgc aattacagtt
     1081 aatgttgcac ctaattttgg caataacaat aaattgccag ttaaaaagct aaccccaagc
     1141 aaaccaccga ctacccatgt gtagttaaat gattgattat tgtaaaagtg aatagtaaat
     1201 acttctggat tgataatgat atttaaaata attaaacaaa ttgttccaac tgaaaatgaa
     1261 atgaatgaag tatagaaagg cgatttagta tacagtgata gtcttgaatt gacagatgtt
     1321 tggataggaa taagcattcc aactaggaca ccaatgatat agaaaagaag catatgaata
     1381 aagtctcact ttccaaagtt aataataaaa tcatatat
//
LOCUS       DAXWFK010000304         1383 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.309, whole genome shotgun sequence.
ACCESSION   DAXWFK010000304 DAXWFK010000000
VERSION     DAXWFK010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.309"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(29..1156)
                     /locus_tag="ACGEXS_002725"
     CDS             complement(29..1156)
                     /locus_tag="ACGEXS_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFF8013672.1"
                     /translation="MKSKYEPLFDKVELPNGVELRNRFVLAPLTHISSNDDGTISDVE
                     LPYIEKRSQDVGITINAASNVSDVGKAFPGQPSIAHDSNIEGLKRLATAMKKNGAKAL
                     VQIHHGGAQALPELTPDGDVVAPSPISLKSFGQKQEHSAREMTNEEIEQAIKDFGEAT
                     RRAIEAGFDGVEIHGANHYLIHQFVSPYYNRRNDVWANQYKFPVAVIEEVLKAKEAYG
                     NKDFIVGYRLSPEEAESPGITMEITEELVNKISHMPIDYIHVSMMDTHATTREGKYAG
                     QERLPLIHKWINGRMPLIGIGSIFTADEALDAAENVGVDLVAIGRELLLDYQFVEKIK
                     DGREDEIINYFDPEREDNHHLTPNLWHQFNEGFYPLPRKDK"
BASE COUNT          404 a          279 c          202 g          498 t
ORIGIN      
        1 aatatcatta gtggtaacag ttaaacactt atttatcttt acgtggtaat ggataaaacc
       61 cttcattaaa ttgatgccat aaattaggag ttaagtgatg attatcttct ctctctggat
      121 caaagtaatt aataatttca tcttcccgtc catctttaat tttttcaaca aattgataat
      181 ccagtagtag ctctctacca atggctacta agtcaacacc aacattttca gctgcatcta
      241 aagcttcgtc agctgtgaaa attgaaccaa taccgataag tggcatacga ccatttatcc
      301 atttgtgaat taaaggcagt ctttcttgtc cagcgtattt accttcacgt gtcgttgcat
      361 gcgtatccat cattgaaaca tgaatatagt cgattggcat atggctaatt ttattaacga
      421 gttcctctgt aatttccatt gtgattcctg gagactccgc ttcctctgga gataatctgt
      481 atccaactat aaagtcttta ttgccatacg cttctttcgc tttaagtact tcttcaatca
      541 cagcgaccgg gaatttatat tgatttgccc atacatcatt tcttctatta tagtatggtg
      601 atacaaattg atgaattaag taatgattcg cgccatgtat ttcaacacca tcaaaccctg
      661 cttcaattgc acgtcgcgtt gcttcaccaa aatccttgat tgcttgttca atctcttcat
      721 tcgtcatttc tctagcacta tgttcttgtt tctgaccaaa actttttaaa gaaattggac
      781 ttggtgctac gacgtctcca tcaggtgtta attcaggcaa tgcttgtgca ccgccatgat
      841 gtatttgtac gagtgctttg gcaccgtttt tcttcattgc tgtagctaat cgttttagtc
      901 cttcaatatt actgtcatgc gcgattgatg gctgtcctgg aaatgctttt ccgacatcac
      961 tcacattact cgcagcatta attgtaatac caacatcttg tgaacgcttt tcaatataag
     1021 gaagttctac atctgaaata gtaccatcat catttgaaga aatatgtgtt aaaggggcta
     1081 acacaaatcg atttctcaac tctactccat ttggtaattc tactttatca aacaatggtt
     1141 cgtatttact tttcattact taattcctcc ttaaaattat tgagttgact ataacatgta
     1201 attttttcat tctccaatta tctgtttata tagttgcaat tcgtttaata ctttaagttg
     1261 tagttctcat ttctaaaata aataaaaaaa acaaatccac atacaatatt gcaaatcaac
     1321 gattctatct aacaaagata atggatatgt caccttaaac catttgtacg tgtaaataga
     1381 atc
//
LOCUS       DAXWFK010000305         1337 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.310, whole genome shotgun sequence.
ACCESSION   DAXWFK010000305 DAXWFK010000000
VERSION     DAXWFK010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.310"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(136..636)
                     /locus_tag="ACGEXS_002726"
     CDS             complement(136..636)
                     /locus_tag="ACGEXS_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HFF8013673.1"
                     /translation="MKKLVTATTLTAGIGTALVGQAHHADAAENYTNYNNYNYNTTQT
                     TTTTTTTTTTSSISHSGNLYTAGQCTWYVYDKVGGEIGSTWGNANNWAAAAQGAGFTV
                     NHTPSKGAILQSSEGPFGHVAYVESVNSDGSVTISEMNYSGGPFSVSSRTISASEAGN
                     YNYIHI"
     gene            complement(873..1295)
                     /locus_tag="ACGEXS_002727"
     CDS             complement(873..1295)
                     /locus_tag="ACGEXS_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HFF8013674.1"
                     /translation="MTTEMNDVTNHQPNRKSNDENIMAMLIYLLSLFTSIIGPLIIWL
                     LKKDESKLVDRAGKNYLNYTISYIIWSIVLVVITLIGVFLIATDIDFIIIIGFIITFI
                     GILSIFAFSILSFVFTIIACVKYYNGQEYIIPLTIRFI"
BASE COUNT          463 a          195 c          222 g          457 t
ORIGIN      
        1 gaggaaatat aaataagtag gtggcaacga aaaatagcaa aaaaagagct tctcctataa
       61 aggaaagctc aaagtttttt gatgacatat gtactagaat taagtttcaa gacaatatgt
      121 atcatcgtgt ttatattaaa tatggatgta gttgtagtta cctgcttcac ttgcagaaat
      181 agttctagaa cttactgaga aaggtccgcc actataattc atttctgaaa ttgtaactga
      241 accatcactg tttacacttt ctacatatgc aacgtgacca aatggtcctt cagaagattg
      301 taggatagcg cctttagaag gtgtatgatt tactgtgaat ccagcacctt gtgcagcagc
      361 agcccaatta ttagcatttc cccaagtaga accgatttct ccgccaactt tatcatatac
      421 ataccaagta cattgtcctg cagtgtataa gttaccagaa tgtgaaattg atgatgtagt
      481 tgtcgtagtt gtcgtagtcg ttgtagtttg agtcgtgttg tagttatagt tgttgtaatt
      541 tgtataattt tcagcagcat ctgcatgatg tgcttgacct actaatgctg tgccgattcc
      601 tgctgttaac gtagttgctg ttactaattt tttcatgaat aaagtcctcc aaagttctat
      661 atcttttttt ataaataaaa cgtagcgact gttttattct cacatctcga attgatgaca
      721 atagttactt taacaaaatt aatgcttctt gtggggaatg ttattgattt gtaaaagaat
      781 aaaaaaactt tgactaattt tgtaataaaa attagtcaaa gttacaatga gattaacaga
      841 taattaatag gaaatattta tttgtaatat gtttaaataa atcgaattgt taaaggtatt
      901 atatattctt ggccattata atatttgaca cacgcaataa ttgtgaatac aaaagataat
      961 attgagaaag cgaatatgga taaaataccg ataaacgtaa tgatgaaacc tataataata
     1021 atgaaatcaa tatctgtagc aattaggaaa acgcctatta aagtgataac gactaaaacg
     1081 atagaccaaa taatataaga aatcgtatag ttaagataat tttttccagc acgatcaact
     1141 agtttcgatt catctttttt caataaccat attatcagtg gaccaataat agatgtgaat
     1201 aaacttaata aatagataag catcgccata atgttctcat cattggattt gcgattcggt
     1261 tgatgatttg ttacgtcgtt catttcagtt gtcatattag acactccttt gaaaattgta
     1321 atattatctt taactat
//
LOCUS       DAXWFK010000306         1316 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.311, whole genome shotgun sequence.
ACCESSION   DAXWFK010000306 DAXWFK010000000
VERSION     DAXWFK010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.311"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            120..872
                     /gene="sel26"
                     /locus_tag="ACGEXS_002728"
     CDS             120..872
                     /gene="sel26"
                     /locus_tag="ACGEXS_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001804768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type 26"
                     /protein_id="HFF8013675.1"
                     /translation="MGEFEVKYLTGFILILLLEGIFTNSASAIEYSDLHHKSKFDSKR
                     LSNAKMSFINPTQLENKNTNDRLLKHDLLFHDMFVNDDWKKDFKVEFENEALSKKFIN
                     KDIDIFAGNYGYGCHGGATNKTQCSYGGVTLSDNNKYDDYKNIPCNLWIDGHQTEIEL
                     TAVKTKKKIVTIQELEVQLRNYLNEKYKLYEQGGDIVKGYVKYYNDDEQNVEYDFYNL
                     NGEYGREVLKMYADNKTINSDKLHLDIYLFKS"
     gene            complement(1074..>1316)
                     /locus_tag="ACGEXS_002729"
     CDS             complement(1074..>1316)
                     /locus_tag="ACGEXS_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013676.1"
                     /translation="VTKIATYGSFIAIASFVILFISIFLKFSFNIEFTATIMNICRYT
                     LILGFILMSLPDVVDKNIKKIIFDAFIIIVFIFFLL"
BASE COUNT          527 a          147 c          220 g          422 t
ORIGIN      
        1 aaaagtatta tgagcgtgat acataatcaa aattgtaggc tcaagaacca ctacataata
       61 aaccataagc ggttctttat catttatgtc tcgctctcaa atgtaaatta ataattgttt
      121 tgggggagtt tgaagttaaa tatttaacag gatttatttt aatattattg ttagaaggaa
      181 tttttacaaa ttcagcgagt gcaatcgaat attcagactt acatcataaa agtaagtttg
      241 attcaaagcg tctaagtaat gctaagatgt cattcatcaa tccaactcag cttgaaaata
      301 aaaacacaaa cgatagactg ttgaagcatg atttgttatt tcatgacatg ttcgtaaatg
      361 atgattggaa aaaggatttt aaagttgaat ttgaaaatga ggcactttca aagaaattta
      421 taaataagga tatcgatata tttgctggaa attatggata cggatgtcat gggggagcaa
      481 ccaataaaac gcaatgtagt tatggtggtg ttactttaag tgacaataat aaatacgatg
      541 attataagaa tataccttgt aatttatgga ttgacggaca tcaaacagaa atagaactaa
      601 ctgcagtaaa aacgaaaaag aaaattgtta ctattcaaga attagaggtt caattaagaa
      661 attatttgaa tgagaagtat aagttgtacg aacaaggtgg cgacattgtt aaagggtatg
      721 ttaaatatta taatgatgat gaacaaaatg tagaatatga tttttataat ttaaatggtg
      781 agtatggtcg tgaggtatta aaaatgtatg ctgataataa aactatcaat agtgacaaat
      841 tgcatttaga tatctattta ttcaaatcat aatttaatta agttaatcat taataatctt
      901 tgacagtgaa atataaagta cctgtcatta tgttgttaca aataaaaaca gcagtaagga
      961 tgctaacact tagaaaagtc ttactgctgt tttctattag aattatcttt aatgcacaaa
     1021 ggaattcgat acgaaacaaa tatgattaac tgtgtaatta caaaataaaa ctactatagt
     1081 aaaaagaaga taaagacgat gataataaat gcatcgaata taattttctt gatgttttta
     1141 tcaacaacat caggtaagga cataagtata aatcctagaa ttagagtgta gcgacatatg
     1201 ttcataattg ttgctgtaaa ctcgatatta aatgaaaact ttaaaaatat acttataaat
     1261 aaaatgacaa acgacgcaat tgcaataaaa ctgccgtatg tagctatctt tgttac
//
LOCUS       DAXWFK010000307         1302 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.312, whole genome shotgun sequence.
ACCESSION   DAXWFK010000307 DAXWFK010000000
VERSION     DAXWFK010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.312"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            255..>1302
                     /locus_tag="ACGEXS_002730"
     CDS             255..>1302
                     /locus_tag="ACGEXS_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFF8013677.1"
                     /translation="MSNANNADNKQFFSKILNSIGAGVVIALVPNALLGEVLKIFKSG
                     NEILELTYQLVILIQSFMAFIIGVLAAHQFKFNGAGAAIVGTSAMIGSGAVVYSNNSF
                     MLKGIGDIINTSLVVIIACLIYMVLQNKLGSFELIILPVLVPIVSGGIGLITLPYIRK
                     ITQAIGNVIHSFTDLNPLLMSILISVAFSLLMVTPISLVAIATAISLNGLGSGAANLG
                     IVAACVTFLFGSLRVNSIGVNAVLLIGAAKMMIPVYLKNLIISIPLTINGIITGIIAY
                     VLQVKGTPLSAGFGYTGLVGPINAFNRMSGDPTMNIILLALGYFVIPFVSAFIVHELC
                     KKFIPIYSNDIYKFE"
BASE COUNT          408 a          158 c          228 g          508 t
ORIGIN      
        1 accaagtcga ttcaatttat ctatcttaat agtaatatgg ctattttaat atttacaatt
       61 tattacatta ttagtcacag gatgtaatta gtagtatttt aaaattaaca tgacattgat
      121 tagtgagtag attgtttaaa tttgctttat tcaatttgtg gattgttata tttagattaa
      181 attagccaac taatcattgt taaagttaat tttctataat atgatgagtt taatgttcaa
      241 attggaggtc gtttatgagt aacgcaaata atgctgataa taaacagttt ttcagtaaga
      301 ttttaaattc tattggtgct ggtgttgtta ttgcacttgt tccgaatgct ttattaggtg
      361 aagtattaaa aatatttaaa agtggtaatg aaattttaga attaacgtat cagttagtga
      421 tactaattca atcatttatg gcatttatta ttggggtttt agctgctcat caatttaaat
      481 ttaatggtgc aggtgctgct atagtaggta catcagctat gattggttca ggtgctgttg
      541 tatatagcaa taatagtttt atgttaaaag gtataggtga cattataaat acgagtttgg
      601 ttgttattat tgcatgtctt atatatatgg tattacaaaa caaacttgga tcttttgaat
      661 taattatatt accagtttta gtacctatcg ttagtggggg tatcggtcta atcacattac
      721 cttacattcg aaaaattact caagcaattg gaaatgtaat tcattctttt acagacttaa
      781 atccgttgtt aatgtctata cttattagtg tggcattttc attattaatg gtaacaccaa
      841 tatcgttagt agcgattgca actgcgatta gtttaaacgg actaggaagt ggagcagcaa
      901 acctaggtat agtggctgcc tgtgttactt tcttatttgg ctcattgcgt gtcaattcta
      961 ttggtgtaaa tgcagtttta ctcataggag ctgcaaaaat gatgatccca gtttacttga
     1021 aaaatttaat tatttcgata ccattgacta taaatggaat aattactgga atcattgctt
     1081 acgttcttca ggttaaagga acaccattgt ctgctggatt tggatataca ggattagttg
     1141 gcccaataaa tgcatttaac cgaatgtcag gtgatccaac aatgaatatt attttattag
     1201 cacttggtta ttttgtcata ccatttgtat cggcatttat tgttcatgaa ttgtgtaaaa
     1261 aattcattcc tatatattct aatgatattt ataaatttga ag
//
LOCUS       DAXWFK010000308         1297 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.313, whole genome shotgun sequence.
ACCESSION   DAXWFK010000308 DAXWFK010000000
VERSION     DAXWFK010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.313"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            183..497
                     /locus_tag="ACGEXS_002731"
     CDS             183..497
                     /locus_tag="ACGEXS_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013678.1"
                     /translation="MYFVLAIFTIISASVSLGYSIQACASSHNINAYYALSRSLPLFL
                     LAIFSLVIHSAIFLITISIAMILVQFLDAIVGYKSEDVFKTYGPLAISVVNLILLIVF
                     LF"
     gene            complement(503..640)
                     /locus_tag="ACGEXS_002732"
     CDS             complement(503..640)
                     /locus_tag="ACGEXS_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFF8013679.1"
                     /translation="MMVDKATTQIQHDCGIRVLVFIKLIESYIKYSLDNSILVRLRFV
                     V"
BASE COUNT          461 a          187 c          180 g          469 t
ORIGIN      
        1 tgtttgttat taataaattt gcataatgaa aaatttcaac aaattcagtt tatgacatag
       61 ttataatcat ttgaaaccat tataatgata acaaccagtt tctcataaat ttattctaat
      121 tatcttttaa ataactttag acaagtactt gtttatatcc aaatatagaa agaaggatta
      181 acatgtattt tgttttagca atatttacaa taatcagtgc cagtgtaagt ttaggttatt
      241 caattcaagc atgtgcatct agccataata taaatgcata ttatgcactt agtcgaagct
      301 tacctttatt tttattagct attttttctt tagtcattca tagtgctata tttttgataa
      361 ctatatccat tgcaatgatc ttagttcaat ttttagatgc gattgttggt tataaaagtg
      421 aagatgtctt taaaacttat ggtccattag caatatctgt agtgaactta atattattaa
      481 tagttttctt attttaactc acttatacaa cgaatcttaa tcgaactaat atcgaattat
      541 ctaaagaata tttgatgtag ctttcaatta atttaataaa gaccagcact ctaatgccac
      601 aatcatgttg tatttgtgtt gtcgctttat ccaccatcaa tgattatttt ttacaccaat
      661 caaaaaatcg aactgatata aataagtaca aagcttatct atcaatccga tttagttata
      721 aaacaaaaaa agccacagta atgtggcttt ttgttatatt cagtatcaaa atggtatcaa
      781 tagccatttt cggaagtcaa gaatggctta acaacgcggt ttaaagctat ccaatactac
      841 cttccatttc gattgaaaaa actaattttt aaggacgttt ttttatagaa acgttgattt
      901 aatgcgattt aaaatgaagt tatttctctt gaaattttga ggctattatt ttttggtatc
      961 aaaaatggta tcatttatag ttattttagc ttcacatata aaaaaccaca ctcctaaatt
     1021 aataggtggt gtggtttgat catttataat ataacataaa aaacaaccac ccagtaacta
     1081 gtatgggtgg tttaaatatg cagtcagctt cttactgctt tacgcaagta agtcctatgc
     1141 atagccggat tgactaccgg aaatgtggtt ttaagccaga ttggttactg gtaatgtaat
     1201 tacattataa caaaaaaaat aggcaagtac cgaagtacct gcctaaataa caacaagatt
     1261 aacatgtgaa taatggaaat aaaaagtcag cccgaag
//
LOCUS       DAXWFK010000309         1270 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.314, whole genome shotgun sequence.
ACCESSION   DAXWFK010000309 DAXWFK010000000
VERSION     DAXWFK010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.314"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            189..1214
                     /locus_tag="ACGEXS_002733"
     CDS             189..1214
                     /locus_tag="ACGEXS_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HFF8013680.1"
                     /translation="MISFENDYLEGAHEKVLKRLVDTNLVQASGYGFDQFTAQAIEKI
                     KDTIDCPNATIRFLVGGTQTNQVVINSMLESYEGVISADTGHVAVHEGGAIEYSGHKV
                     ITIPSKEGKVSASDVETYMETFKSDFKKDHMVFPGMIYISHPTEYGTLYSKSELEELC
                     KVCKQYQLPLFMDGARLGYGLMSDQSDMTIKDIAKYCDVFYIGGTKIGALCGEAIVFT
                     KNNEPKQFTTRIKHHGALLAKGRLTGIQFLELFTDNLYFNISRHAIEMANKMKDGFKN
                     KGYRLYFDSPTNQQFFILSNEKIAELEQKVKFAVWEKYDDQHRVVRFATSWATTEENL
                     NKLLELI"
BASE COUNT          460 a          166 c          237 g          407 t
ORIGIN      
        1 tgaaaagtcg ctgtgataaa aaaataatta attttcataa tgtattgttt gatgtataat
       61 atatttattc aacttcgcga taatgcgata caaactttaa ccattcataa tacattttaa
      121 atatcgaact agttttgaaa ttggtttatc ttattaaaat gttgatatta gttttagggg
      181 gaaatgtagt gatttcattc gaaaatgatt atttagaagg tgctcatgaa aaagtattaa
      241 aacgattagt agatacaaat cttgtacaag cttcgggata tggctttgat caattcacag
      301 ctcaggcaat tgaaaagatt aaagatacaa ttgactgtcc aaatgctaca attcgttttt
      361 tagtaggtgg aacacaaacc aatcaggttg ttattaattc aatgttagaa agttacgaag
      421 gggtcatttc agcagacact ggtcatgttg ctgtgcatga gggcggtgca attgaatata
      481 gtggacataa agtaattact ataccctcta aggaaggaaa agttagtgct tcagatgttg
      541 aaacttacat ggagacattt aaaagtgatt ttaaaaagga ccatatggta ttcccaggaa
      601 tgatatatat ttctcatcct acagaatatg gcacattata ctctaaatca gaattggaag
      661 agttatgcaa agtatgtaag caatatcagc ttccattatt tatggatggt gcacgattag
      721 ggtatggatt gatgagtgac caatcagata tgactattaa agacatcgct aaatattgtg
      781 atgtatttta tataggtggt acgaagatag gtgcattatg cggggaagcc attgttttta
      841 cgaaaaataa tgaaccgaaa caatttacca ctcgaatcaa gcatcacggt gcccttttag
      901 caaaaggacg gttaacaggc atacaatttt tagaattatt tacggacaat ttatatttta
      961 atataagcag acatgctatc gaaatggcaa ataagatgaa agacgggttt aaaaataaag
     1021 gctatcgtct ttactttgat tctccaacca atcaacaatt ttttatttta agtaacgaga
     1081 aaatagcaga gttagaacaa aaagttaaat ttgctgtttg ggaaaaatac gatgatcaac
     1141 atcgtgttgt tagattcgca accagttggg cgacaaccga agaaaattta aataaactat
     1201 tagaattaat ctaataatgc tgttatatgt catctggtaa atcatttatt aattaattaa
     1261 gatcagttga
//
LOCUS       DAXWFK010000310         1269 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.315, whole genome shotgun sequence.
ACCESSION   DAXWFK010000310 DAXWFK010000000
VERSION     DAXWFK010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.315"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            66..551
                     /locus_tag="ACGEXS_002734"
     CDS             66..551
                     /locus_tag="ACGEXS_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFF8013681.1"
                     /translation="MRKFLSKTHHHTNPLWRVYRLVKFSKVFKNVIIIEFSKFIPSMV
                     LKRHIYKQLLNINIGNQSSIAYKVMLDIFYPELITIGSNSVIGYNVTILTHEALVDEF
                     RYGPVTIGSNTLIGANATILPGITIGDNVKVAAGTVVSKDIPDNGFAYGNPMYIKMIR
                     R"
     gene            559..>1269
                     /locus_tag="ACGEXS_002735"
     CDS             559..>1269
                     /locus_tag="ACGEXS_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFF8013682.1"
                     /translation="MAQKNNNVIPMTFDDAFYRKMAKQKFKQREYKRAAEYFEKVLEL
                     SPDDLEIQIDYAQCLVQLGIAKKAEHLFYDNIIYNRHLEDSFYELSQLNIEVNEPNKA
                     FLFGINYVIVSDDQDYRDELDQMFDVKYQSEEQIELEAQLFVVQILFQYLFSQGRLKD
                     AKNYVLHQPQEVQDHRVVRNLLAMCYLYLGEYDTAKALYEALLQEDSTDIYALCHYTL
                     LLYNTKENEQYQKYLKILN"
BASE COUNT          460 a          170 c          225 g          414 t
ORIGIN      
        1 tccaccgttg tatagcaaag ttggggcgct tccatggcca acaaaaaaag tgaagtagtg
       61 atagtttgag gaaattttta tcaaaaacac atcatcatac aaacccttta tggcgtgtat
      121 accgtcttgt taaattttcg aaagttttta agaatgtaat tatcattgaa ttttcgaaat
      181 ttattccaag tatggtactg aaaagacata tatataaaca acttttaaat attaatatcg
      241 gtaatcaatc gtcgatagct tataaagtaa tgttagatat tttttaccca gaactgatta
      301 cgattggtag taacagtgtt attggttaca atgtaacaat tttgacgcat gaagcattag
      361 ttgatgaatt tcgttatgga ccagtgacga taggatctaa cactttgatt ggtgcaaatg
      421 ctaccatttt acccggtata acgattggtg acaatgtaaa agttgcagct ggtacggttg
      481 tttcaaaaga tataccggat aatggatttg catatggcaa ccctatgtat ataaaaatga
      541 ttaggaggtg acaattttat ggcgcaaaag aataataatg taattccaat gacttttgat
      601 gatgcatttt atcgtaaaat ggctaaacag aagtttaaac aaagagaata taaacgagct
      661 gctgaatact ttgaaaaagt gttagaattg tcacctgatg atctggaaat tcaaattgat
      721 tatgcacaat gtctagtgca acttggtatt gctaaaaaag cagaacattt attttatgac
      781 aatattattt ataataggca tctagaagat agcttttatg aattgagtca gctcaacatt
      841 gaagttaacg aaccaaacaa ggcattcttg tttggtatta attatgttat tgttagcgac
      901 gaccaagatt atagagatga attagatcaa atgtttgatg tgaaatatca aagtgaagaa
      961 caaattgaac ttgaagctca attgtttgta gttcaaatac tattccaata tcttttttct
     1021 caaggtcgat taaaagatgc aaagaattat gtcttacatc aaccacaaga agttcaagat
     1081 catcgtgtag tacgtaattt attggcaatg tgttatttat atctcggtga atatgatacg
     1141 gctaaagcat tgtacgaagc actattacaa gaggatagta cagatatata tgcattatgc
     1201 cattatactt tgctacttta taacactaag gaaaatgaac aatatcaaaa atatttaaaa
     1261 atattaaac
//
LOCUS       DAXWFK010000311         1263 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.316, whole genome shotgun sequence.
ACCESSION   DAXWFK010000311 DAXWFK010000000
VERSION     DAXWFK010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.316"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..>1263
                     /locus_tag="ACGEXS_002736"
     CDS             <1..>1263
                     /locus_tag="ACGEXS_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFF8013683.1"
                     /translation="LYFYLMLFFSTTIIGALLLYLPFTGKKPISFLDALFIASSAFTV
                     TGLSPVDIGSQFNILGEIVILLLIQIGGLGIVTVTLLTLVFLNRKISMKNRFLIMVTW
                     NIDEPGGVIKLIKHLAIYSLVTELIGMICLCLSFIPKFGIGKGLFLSLFTSVSAFNNA
                     GFALFKNNLIDYSSDPIVIITISILIIFGGIGHFVVIDFINCKKLSKLSLHSKLVLTT
                     TSILIIIGAITFFLLEQFNTMQHMGLVEKIGNSFFQSVTTRTAGFNSIDIASINKSTA
                     LMLMLLMFIGGAPLSAAGGIKITTFAVAFIFVLNYIRKENNVSVFNKEISDKHIKLSI
                     VTINISFLFISIITFILSIINPNISLIKLLFEVVSAFGTVGLSMNLTTEYHGITKIII
                     IFVMLCGKVGLLTLLRTFIPPKSPKNYR"
BASE COUNT          418 a          181 c          182 g          482 t
ORIGIN      
        1 tttatatttt tatttaatgc ttttcttctc tacaactatc ataggtgcac ttttattgta
       61 tttacctttt actggtaaaa agccaatatc ttttttagat gccctattta tagcttcaag
      121 tgcatttaca gttactggct tgtccccagt tgatatagga tcacagttta atatacttgg
      181 tgagatagta atactattat taattcaaat aggtggtctg ggtatcgtga ccgtaaccct
      241 attgacacta gtatttttaa atagaaagat atcaatgaaa aatagattct tgattatggt
      301 tacatggaat attgacgaac ctggtggtgt tattaagcta attaaacact tggctattta
      361 tagtttagtc actgaattaa ttggtatgat ttgtttgtgt ttatctttta taccaaaatt
      421 tggtataggc aaaggtttat ttttaagctt attcacatca gtatcagctt ttaataatgc
      481 tggatttgcc ctttttaaga ataacttaat agattattct agtgatccaa ttgtcattat
      541 tacaatctca atactcataa tatttggagg tattggacat tttgtcgtga tagactttat
      601 taattgtaaa aaattgagta aattatcttt acattctaaa ttagtcttaa ctacaactag
      661 tatcctaata attataggag ctattacatt ctttttatta gaacagttta atactatgca
      721 acatatggga ctagttgaaa aaatcggaaa ttcttttttc caatcagtaa caacacgaac
      781 agcgggtttt aacagtatag atatagcaag cattaacaaa tctaccgcat taatgttaat
      841 gctacttatg tttattggtg gtgcccctct cagtgcagct ggaggaatta aaataactac
      901 ttttgcagtt gcgtttattt ttgtactaaa ttatatacgt aaagaaaata atgtttcagt
      961 attcaataaa gaaatatctg acaaacatat aaaactatct attgttacca ttaatatctc
     1021 atttctattt atcagcatca ttacttttat attatcgata attaatccga acatatcatt
     1081 aatcaagtta ttattcgaag tggtttctgc attcggaaca gtagggttaa gtatgaacct
     1141 taccacagaa tatcatggta ttactaaaat aattattata ttcgttatgc tttgcggtaa
     1201 agtaggacta ttaactttat taagaacatt tataccacca aaaagtccta aaaattaccg
     1261 cta
//
LOCUS       DAXWFK010000312         1260 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.317, whole genome shotgun sequence.
ACCESSION   DAXWFK010000312 DAXWFK010000000
VERSION     DAXWFK010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.317"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            9..926
                     /gene="truB"
                     /locus_tag="ACGEXS_002737"
     CDS             9..926
                     /gene="truB"
                     /locus_tag="ACGEXS_002737"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFF8013684.1"
                     /translation="MYNGILPVYKERGLTSHDVVFKLRKILKTKKIGHTGTLDPEVAG
                     VLPVCIGNATRVSDYVMDMGKAYEATVSIGRSTTTEDQTGDTLETKGVHSADFNKDDI
                     DRLLESFKGIIEQIPPMYSSVKVNGKKLYEYARNNETVERPKRKVNIKDIGRISELDF
                     KENECHFKIRVICGKGTYIRTLATDIGVKLGFPAHMSKLTRIESGGFVLKDSLTLEQI
                     KELHEQDSLQNKLFPLEYGLKGLPSIKIKDSHIKKRILNGQKFNKNEFDNKIKDQIVF
                     IDDDSEKVLAIYMVHPTKESEIKPKKVFN"
     gene            941..>1260
                     /locus_tag="ACGEXS_002738"
     CDS             941..>1260
                     /locus_tag="ACGEXS_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFF8013685.1"
                     /translation="MKVIEVTHPIQSKQYITEDVAMAFGFFDGMHKGHDKVFDILNEI
                     AEARSLKKAVMTFDPHPSVVLNPKRKRTTYLTPLSDKIEKISQHDIDYCIVVNFSSRF
                     ANVSV"
BASE COUNT          480 a          167 c          250 g          363 t
ORIGIN      
        1 gtgactatat gtataatggg atattaccag tatataaaga gcgcggttta acaagtcatg
       61 acgttgtatt caaattgcgt aaaatattaa aaactaaaaa aataggtcac acgggtacgc
      121 ttgatcccga agttgcaggc gtgttaccgg tatgtatagg taatgcaacg agagttagtg
      181 attatgttat ggatatgggc aaagcttatg aagcaactgt atcgatagga agaagtacaa
      241 cgactgaaga tcaaacgggt gatacattgg aaacaaaagg tgtacactca gcagatttta
      301 ataaggacga tattgaccga ttgttagaaa gttttaaagg tatcattgaa caaattccgc
      361 cgatgtactc atccgtcaaa gtaaatggta aaaaattata tgaatatgcg cgtaataatg
      421 aaacagttga aagaccaaag cgtaaagtta atattaaaga cattgggcgt atatctgaat
      481 tagattttaa agaaaatgag tgtcatttta aaatacgcgt catctgtggt aaaggtacat
      541 atattagaac gctagcaact gatattggtg tgaaattagg ctttccggca catatgtcga
      601 aattaacacg aatcgagtct ggtggatttg tgttgaaaga tagccttaca ttagaacaaa
      661 taaaagaact tcatgagcag gattcattgc aaaataaatt gtttccttta gaatatggat
      721 taaagggttt gccaagcatt aaaattaaag attcgcacat aaaaaaacgt attttaaatg
      781 ggcagaaatt taataaaaat gaatttgata acaaaattaa agaccaaatt gtatttattg
      841 atgatgattc agaaaaagta ttagcaattt atatggtaca ccctacaaaa gaatcagaaa
      901 ttaaacctaa aaaagtcttt aattaaagga gatagaattt atgaaagtca tagaagtgac
      961 acatcctata caatctaaac agtatattac agaggatgtt gcaatggcat tcggattttt
     1021 cgatggcatg cataaaggtc atgacaaagt ctttgatata ttaaacgaaa tagctgaggc
     1081 acgcagttta aaaaaagcgg tgatgacatt tgatccgcat ccgtctgtcg tgttgaatcc
     1141 taaaagaaaa cgaacaacgt atttaacgcc actttcagat aaaatcgaaa aaattagcca
     1201 acatgatatt gattattgta tagtggttaa tttttcatct aggtttgcta atgtgagcgt
//
LOCUS       DAXWFK010000313         1248 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.318, whole genome shotgun sequence.
ACCESSION   DAXWFK010000313 DAXWFK010000000
VERSION     DAXWFK010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.318"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..223
                     /locus_tag="ACGEXS_002739"
     CDS             <1..223
                     /locus_tag="ACGEXS_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HFF8013686.1"
                     /translation="LEPWTSCEFDFTRDGKLNVSFDYIDWVNSEFGPMGREHYYMYKK
                     FGIWPEKEYAINWVEKIKDYVKEQDEAEL"
     gene            234..734
                     /locus_tag="ACGEXS_002740"
     CDS             234..734
                     /locus_tag="ACGEXS_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin YezG family protein"
                     /protein_id="HFF8013687.1"
                     /translation="MTFEGKLSQMYNEIANEISGMIPVEWEQVFTIAYVTDQAGEVIF
                     NYTKPDSDELNYYSDIPKDCNVSKDIFKNSWFKVYRMFDELRETFKEEGLEPWTSCEF
                     DFTRDGKLNVSFDYIDWINTEFDQLGRQNYYMYKKFGVIPEMEYEMEEVKEIEQYIKE
                     QEEAEQ"
     gene            745..1245
                     /locus_tag="ACGEXS_002741"
     CDS             745..1245
                     /locus_tag="ACGEXS_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HFF8013688.1"
                     /translation="MTFEEKISKLYNEIANEISSMIPVEWEKVYTMAYIDDGGGEVFF
                     NYTKPGSDDLNYYTDIPKEYNISVQVFDDLWMDLYDLFEELRDLFKEEGLEPWTSCEF
                     DFTSEGKLKVSFDYIDWINTEFDQLGRENYYMYKKFGVLPEMEYEMEEIKEIDQYIKE
                     QDEAEI"
BASE COUNT          491 a          117 c          268 g          372 t
ORIGIN      
        1 gcttgaacca tggacatcat gcgaatttga ctttacaaga gatggcaaat tgaatgtatc
       61 ttttgattat attgattggg tgaattcaga atttggacca atgggaagag aacattatta
      121 tatgtataaa aaatttggaa tttggcctga aaaagaatat gccataaatt gggttgaaaa
      181 aataaaagat tatgttaaag agcaagatga agctgaacta taggggagat aatatgactt
      241 tcgaaggaaa actaagtcaa atgtacaacg agattgcgaa tgagattagt gggatgatac
      301 cggtagaatg ggagcaagta tttacaatag cttatgtaac tgatcaagct ggagaagtca
      361 tctttaatta tactaaacca gatagtgatg aattaaatta ttattcagac atacctaaag
      421 attgcaatgt ctcaaaagat atttttaaga attcatggtt taaagtttat cgaatgtttg
      481 atgagttaag agaaactttt aaagaagaag ggcttgaacc atggacatca tgcgaatttg
      541 actttacaag agatggcaaa ttgaatgtat cttttgatta tatagattgg ataaatacag
      601 agtttgatca attgggccgt caaaattatt atatgtacaa aaaatttggg gttataccag
      661 aaatggaata tgaaatggaa gaagttaaag aaatcgaaca atatattaaa gagcaagaag
      721 aagctgaaca atagaggtga taacatgact ttcgaagaga aaataagcaa attatataat
      781 gagattgcga atgagattag cagtatgata ccggtagagt gggaaaaagt atatacaatg
      841 gcttatatag atgatggagg aggtgaagta ttctttaatt atactaaacc aggtagtgat
      901 gacttgaatt attacaccga tatacctaag gagtataaca tctctgtgca agtatttgat
      961 gatttatgga tggatttata tgatttgttt gaggaattaa gagatttatt taaagaagaa
     1021 ggacttgaac catggacatc atgtgaattt gactttacaa gcgaaggtaa attaaaagtt
     1081 tcatttgatt atatagattg gataaataca gagtttgatc aattaggccg tgaaaattat
     1141 tatatgtata aaaaatttgg ggttttacca gaaatggaat atgaaatgga agaaattaaa
     1201 gaaatcgatc aatatattaa agagcaagat gaagctgaaa tatagggg
//
LOCUS       DAXWFK010000314         1241 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.319, whole genome shotgun sequence.
ACCESSION   DAXWFK010000314 DAXWFK010000000
VERSION     DAXWFK010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.319"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..359
                     /gene="lpl5"
                     /locus_tag="ACGEXS_002742"
     CDS             <1..359
                     /gene="lpl5"
                     /locus_tag="ACGEXS_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl5"
                     /protein_id="HFF8013689.1"
                     /translation="LLDKVEDPNLKKRIENFKFFGQYANFKDLENYNNGDVSINWNVP
                     SYDVEYKMSNKDENVKQLRSRYNIPTDKAPMLKMHIDGDLKGSSVGYKRLEIDFSKEG
                     RDISVIDYLSYKPAKK"
     gene            391..1182
                     /gene="lpl6"
                     /locus_tag="ACGEXS_002743"
     CDS             391..1182
                     /gene="lpl6"
                     /locus_tag="ACGEXS_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000456139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl6"
                     /protein_id="HFF8013690.1"
                     /translation="MEYLKRLALLISVIILTIFIMGCDSQSDTAENPKEGSKEAQIKK
                     SFSKTLDMYPIKNLEDFYDKEGYRDGEFKKDDKGTWLIRSEIVKQPKGKVMKTRGMQL
                     YINRNTETAKGFFVLKEISENNNRVNKDKEEKYEVKMVGNKIIPTEQINDEKIKKEIE
                     NFKFFVQYGNFKNFEKYNNGEFSYNPEAPIYSAKYQLHNDDYNVRQLRKRYDISTKET
                     PKLLLKGGGDLKNSSVGQNDIEFTFVERKGENIYFNDSVEFIPSK"
BASE COUNT          530 a          127 c          232 g          352 t
ORIGIN      
        1 tcttattaga caaggtagaa gatccaaatc taaaaaagag aatagaaaac tttaaatttt
       61 tcggacaata tgcaaatttt aaggatttgg aaaattacaa caatggcgac gtgtcaataa
      121 attggaatgt tccaagttat gacgtggaat ataaaatgag caataaagat gaaaatgtta
      181 aacaattaag aagtcgttat aacattccta ctgataaagc tccaatgtta aaaatgcata
      241 ttgacgggga cttaaaaggt agttctgttg gatataaaag gttagaaata gatttttcaa
      301 aagaaggtag ggatatttca gtcattgatt atttaagtta taagccagcg aaaaaatagt
      361 actgataatt aaatattagg gtgtgaagga atggaatatc taaaaaggct tgcattgtta
      421 ataagtgtta ttattttgac catttttata atgggttgtg atagtcaaag cgatactgca
      481 gaaaatccaa aagaaggttc aaaagaagca caaattaaaa agagtttttc gaaaacgtta
      541 gatatgtatc caattaagaa tctcgaggat ttttatgaca aagaaggata tcgagatggc
      601 gaatttaaaa aagatgataa aggtacttgg ctaattagat ccgaaatagt taaacagcca
      661 aagggcaaag tgatgaaaac aagaggtatg caattatata ttaatagaaa taccgaaaca
      721 gccaaaggtt tctttgtttt gaaagaaata agtgaaaata ataatcgtgt aaataaagat
      781 aaggaggaaa aatacgaagt gaaaatggta ggaaataaaa ttattcctac tgaacaaatt
      841 aatgacgaga aaataaaaaa agaaattgaa aacttcaagt tttttgtgca atatggaaac
      901 tttaaaaatt tcgaaaaata caacaatggt gagttttcat ataatcctga agcaccaatt
      961 tattcggcta aatatcaatt acacaacgat gattacaatg taaggcaact acgtaaaaga
     1021 tatgacattt caacaaaaga aacaccgaag ttacttttga aaggtggagg agatttaaaa
     1081 aattcctcag ttggtcaaaa cgatattgaa tttacttttg ttgaaagaaa aggtgagaat
     1141 atttatttta acgatagtgt tgaattcata ccaagtaagt aaagttaaat taaaatgtgt
     1201 ggttagatga aatgatcaaa tatcaaaaaa tgtgcaaaga g
//
LOCUS       DAXWFK010000315         1222 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.320, whole genome shotgun sequence.
ACCESSION   DAXWFK010000315 DAXWFK010000000
VERSION     DAXWFK010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.320"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..748)
                     /locus_tag="ACGEXS_002744"
     CDS             complement(<1..748)
                     /locus_tag="ACGEXS_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFF8013691.1"
                     /translation="MRLQKAPLVTSGLVLGLLGLGNLLKDLSLTLNAVCGIFAFLIWI
                     HLLCTMIKYFNNVKEQLNSPLVSSVFTTFFMSGFLGTTYLNTFFSNITFINSLITPIW
                     ILCLVGIMTHMIIFSIKYLKDFSLENVYPSWTVLFIGIAIAGLTAPVSGYFFIGQLTV
                     IYGFVATCIVLPIVFKRLKAFPLQTSIKPNTSTICAPFSLVAAAYVIAFPKANAFIVI
                     IFLLLAQIFYFYIIIQLPKLLKEPFSPVFSA"
     gene            997..1185
                     /locus_tag="ACGEXS_002745"
     CDS             997..1185
                     /locus_tag="ACGEXS_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFF8013692.1"
                     /translation="MRNQIQKLLDSDLSSLHISKQTGVPQSTIHRMRKNERSLDNMSL
                     KNAELLYKFANSIFSNEN"
BASE COUNT          493 a          162 c          202 g          365 t
ORIGIN      
        1 aagctgaaaa tacgggcgaa aaaggttctt ttagtaattt aggcaattgt ataatgatat
       61 aaaaataaaa tatttgagct aatagtaaaa atataattac gataaaagca ttcgccttag
      121 gaaaagctat aacatatgct gcagcgacta aagaaaatgg tgcacaaatt gtcgatgtgt
      181 tcggtttgat tgacgtctgc aatggaaatg cttttaatcg cttgaaaact ataggtaaga
      241 caatacaagt agctacaaag ccatatatta ctgttaattg acctatgaaa aaatatccgc
      301 taacgggtgc cgtcaatcct gcgatagcaa taccaataaa aagtacagtc cacgaaggat
      361 aaacattttc aagtgaaaaa tcttttaaat attttattga aaaaataatc atatgcgtca
      421 taattcccac aaggcataaa atccaaatag gcgttattaa gctattgata aaagttatgt
      481 tactaaaaaa tgtatttaaa taagtagtac ctaaaaagcc agacatgaaa aatgttgtga
      541 acactgatga aactagagga ctgtttaatt gttctttcac attattaaaa tatttgatca
      601 tagtacataa aaggtgaatc caaatcaaga aagcaaagat tccgcaaaca gcgtttaaag
      661 taagagataa gtcttttaat agattaccca ggcctaataa tcctaagact agtcctgacg
      721 ttactagagg tgctttttga agtctcatga tttacaacct ttcttatgtg attttcttca
      781 ctaattatat catgatagct atggccaatt aataagaaga gtgtgtacta ttacgttatt
      841 agagtatgta tattggattg atagacacat aattgacatt taaatctcaa aattaatgat
      901 atttgaggta tgctttgcaa cctaaatatt ggaatatgtg gaaaagtaat tatttaattt
      961 aaatgatgat tgaagattta caagggggtg tacaaaatga gaaatcaaat tcaaaaacta
     1021 ttagacagtg atttgagcag tttgcatata tcgaaacaaa caggagttcc acaaagcaca
     1081 atacacagaa tgagaaaaaa tgaaagatca ttagacaata tgtcattgaa aaacgctgaa
     1141 ctactttata aatttgccaa tagtatattt agcaatgaaa attaagaaga ataattagtg
     1201 agttaaataa ttaaaaaacc ac
//
LOCUS       DAXWFK010000316         1213 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.321, whole genome shotgun sequence.
ACCESSION   DAXWFK010000316 DAXWFK010000000
VERSION     DAXWFK010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.321"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..444
                     /gene="splF"
                     /locus_tag="ACGEXS_002746"
     CDS             <1..444
                     /gene="splF"
                     /locus_tag="ACGEXS_002746"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplF"
                     /protein_id="HFF8013693.1"
                     /translation="FYNNGGGLYKVTKIVDYPGKEDIAVVQVEEKSTQPKGRKFKDFT
                     SKFNIASEAKENEPISVIGYPNPNGNKLQMYESTGKVLSVNGNIVSSDAIIQPGSSGS
                     PILNSKHEAIGVIYAGNKPSGESTRGFAVYFSPEIKKFIADNLDK"
     gene            509..643
                     /locus_tag="ACGEXS_002747"
     CDS             509..643
                     /locus_tag="ACGEXS_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013694.1"
                     /translation="MHYLICIKANQRLIFTNSIVDWFYLCMNEQLFDIIKNINDFESI
                     "
     gene            807..>1213
                     /locus_tag="ACGEXS_002748"
     CDS             807..>1213
                     /locus_tag="ACGEXS_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HFF8013695.1"
                     /translation="MSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFY
                     RFLSEKAEQEYADALSGEDITYQEAWADEEYREDLKAELIDQVGYFIEPQDLFSAMIR
                     EIETQDFDIEHLATAIRKVETSTLGEESENDFIG"
BASE COUNT          464 a          188 c          203 g          358 t
ORIGIN      
        1 ttttataata acggtggtgg actttataaa gttactaaga ttgtagatta tcctggtaaa
       61 gaagatattg cggttgtaca agttgaagaa aaatcaacac aaccaaaagg tagaaaattc
      121 aaagatttca ctagtaaatt taatatagca tcagaagcta aagaaaatga acctatatca
      181 gtcattggtt atccaaatcc taatggaaat aaactacaaa tgtatgaatc aactggtaaa
      241 gtattatcag tgaatgggaa tatagtgtca tcggatgcaa ttattcagcc tggtagctct
      301 ggttcaccta tattaaatag taaacacgaa gctattggtg taatctatgc cggtaataag
      361 ccatcaggtg aaagcacaag aggatttgct gtttatttct ctcctgaaat taagaaattc
      421 attgcagata atttagataa ataattaaaa cttagacatt cacccaatcc tgacaaaata
      481 tactataact aacatttatt aatatatatt gcattattta atatgcatca aagccaatca
      541 acgattgatt ttcaccaact caattgttga ttggttttat ttatgtatga atgaacaact
      601 ttttgacatc attaagaata taaatgattt tgaaagcatt tgaaagctac aacatttcta
      661 taaaattttt caataacaat tgcgccacta aaactcaaaa tttccaccac caacatccaa
      721 attatcaaca tcgcaacata accaaatgtt ataataaatc tattacacaa agagataaat
      781 tacttatgca aaggcggagg aatcacatgt ctattactga aaaacaacgt cagcaacaag
      841 ctgaattaca taaaaaatta tggtcgattg cgaatgattt aagagggaac atggatgcga
      901 gtgaattccg taattacatt ttaggcttga ttttctatcg cttcttatct gaaaaagccg
      961 aacaagaata tgcagatgcc ttgtcaggtg aagacatcac gtatcaagaa gcatgggcag
     1021 atgaagaata tcgtgaagac ttaaaagcag aattaattga tcaagtcggt tacttcattg
     1081 agccacaaga tttattcagt gcgatgattc gtgaaattga aacgcaagat ttcgatatag
     1141 aacatctagc gacggcgatt cgcaaagttg aaacatctac attaggtgaa gaaagtgaaa
     1201 atgactttat cgg
//
LOCUS       DAXWFK010000317         1195 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.322, whole genome shotgun sequence.
ACCESSION   DAXWFK010000317 DAXWFK010000000
VERSION     DAXWFK010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.322"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(222..>1195)
                     /locus_tag="ACGEXS_002749"
     CDS             complement(222..>1195)
                     /locus_tag="ACGEXS_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013696.1"
                     /translation="NVTLFHCIILILAFLYEFTANRTLNYKLLFSFMIATIGTIIMFS
                     NPNYRKILFEGSEYQQVSNNQGIFSKFAEMISTSLPYGVIFSQIIILSMIAALIIYLL
                     LRSDRYVHLTILKRRIIMIGFITLPLYYLLFYNQFLLNKNTDIGLVGFVNVIVCGYFA
                     FSIFVGIYLSINDRKTQITLYSLLIAICVSASTLVIVTPIEPGNFLIVYTIHVIILII
                     LLKEFRKYKSINVNFIKGASLVLAVIYLSAFIYVHYEHEKRVQLLKQQIKDHPRQEVY
                     TVESLPFEHYMHHPSPINKNYQEWFNNYEELPKMTKVKYIPFGSDDS"
BASE COUNT          460 a          168 c          160 g          407 t
ORIGIN      
        1 tctcatagag tatttataca cgacctaatt tcaaataaaa caagtgccta tatagactta
       61 gctaaaaaat aaaaaagaca taatcatgat tgttcttgat gagcactcca taaagtcagg
      121 ttaaaagtcc aacttttgag atgcacatta tcatttcatg attatgtcta ctttttaata
      181 tttttatgct gctattttaa atttttatag gtaagaataa gttatgaatc gtctgaacca
      241 aatggaatat atttaacttt ggtcattttc ggtaattctt cataattatt aaaccactct
      301 tggtaatttt tattgattgg tgacggatga tgcatataat gttcaaacgg caatgattcc
      361 actgtataaa cctcttgtcg tggatgatct ttaatttgtt gttttaataa ctgaactctt
      421 ttttcatgtt cataatgaac ataaataaat gcactaagat atatcacagc taaaactagt
      481 gatgcacctt taataaaatt aacattgatt gacttatatt tccgaaattc ttttaataaa
      541 ataattaaaa tgattacatg tatcgtataa acaatcaaaa aattacctgg ttctattgga
      601 gtaacaatga ctagtgtcga cgccgaaaca catattgcaa taagtaagga atataaagtg
      661 atttgtgttt tacgatcatt aatagacaaa taaataccta caaatatcga aaacgcaaag
      721 taaccacata caattacgtt cacaaaacca accaatccaa tatctgtatt tttatttaat
      781 aaaaactgat tgtaaaatag taaataataa agcggtaaag tgataaatcc tatcataatg
      841 atacgacgct ttaatattgt taaatgtaca taccgatcac ttctaagcag tagataaata
      901 ataagcgctg caatcatact taaaattata atctgactga aaatgacacc atatggaagt
      961 gacgttgata tcatttctgc aaattttgaa aagatacctt gattatttga aacttgttga
     1021 tattctgatc cttcaaataa aattttgcga taatttggat tggaaaacat aatgattgta
     1081 cctatagtag caatcataaa tgaaaacaat aatttataat taagtgttcg atttgcagta
     1141 aattcataca gaaaagctaa aattaaaatg atacagtgga ataatgtcac attct
//
LOCUS       DAXWFK010000318         1173 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.323, whole genome shotgun sequence.
ACCESSION   DAXWFK010000318 DAXWFK010000000
VERSION     DAXWFK010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.323"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..>1173
                     /locus_tag="ACGEXS_002750"
     rRNA            <1..>1173
                     /locus_tag="ACGEXS_002750"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          297 a          267 c          361 g          248 t
ORIGIN      
        1 cactgccgag aaaagcctct agatagaaaa taggtgcccg taccgcaaac cgacacaggt
       61 agtcaagatg agaattctaa ggtgagcgag cgaactctcg ttaaggaact cggcaaaatg
      121 accccgtaac ttcgggagaa ggggtgctct ttagggttaa cgcccagaag agccgcagtg
      181 aataggccca agcgactgtt tatcaaaaac acaggtctct gctaaaccgt aaggtgatgt
      241 ataggggctg acgcctgccc ggtgctggaa ggttaagagg agtggttagc ttctgcgaag
      301 ctacgaatcg aagccccagt aaacggcggc cgtaactata acggtcctaa ggtagcgaaa
      361 ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa cgatttgggc actgtctcaa
      421 cgagagactc ggtgaaatca tagtacctgt gaagatgcag gttacccgcg acaggacgga
      481 aagaccccgt ggagctttac tgtagcctga tattgaaatt cggcacagct tgtacaggat
      541 aggtaggagc ctttgaaacg tgagcgctag cttacgtgga ggcgctggtg ggatactacc
      601 ctagctgtgt tggctttcta acccgcacca cttatcgtgg tgggagacag tgtcaggcgg
      661 gcagtttgac tggggcggtc gcctcctaaa aggtaacgga ggcgctcaaa ggttccctca
      721 gaatggttgg aaatcattca tagagtgtaa aggcataagg gagcttgact gcgagaccta
      781 caagtcgagc agggtcgaaa gacggactta gtgatccggt ggttccgcat ggaagggcca
      841 tcgctcaacg gataaaagct accccgggga taacaggctt atctccccca agagttcaca
      901 tcgacgggga ggtttggcac ctcgatgtcg gctcatcgca tcctggggct gtagtcggtc
      961 ccaagggttg ggctgttcgc ccattaaagc ggtacgcgag ctgggttcag aacgtcgtga
     1021 gacagttcgg tccctatccg tcgtgggcgt aggaaatttg agaggagctg tccttagtac
     1081 gagaggaccg ggatggacat acctctggtg taccagttgt cgtgccaacg gcatagctgg
     1141 gtagctatgt gtggacggga taagtgctga aag
//
LOCUS       DAXWFK010000319         1159 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.324, whole genome shotgun sequence.
ACCESSION   DAXWFK010000319 DAXWFK010000000
VERSION     DAXWFK010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.324"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..699
                     /locus_tag="ACGEXS_002751"
     CDS             <1..699
                     /locus_tag="ACGEXS_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFF8013697.1"
                     /translation="KFVLYTSALLFISTIQSPIVKGLSMWLAILCLSLLLTFICYLVW
                     TVSYRHRSYNKEVDIIIVLGAGIFTEFVTPMLAARLDRALDIYQQQASATKIIVSGGQ
                     GPDEPISEALAMQRYLIAHGVDKTDILMESHSINTYTNFLYSKQIINNLYHEAVKIVC
                     VTSQFHVLRALRLAQKLGLSIDGVGSHTPYHFFLHVLIKDYLGVMYQYRLLLTLYCSS
                     SWFICLYAAFIYNS"
BASE COUNT          360 a          215 c          181 g          403 t
ORIGIN      
        1 aaatttgtac tctacacatc agcacttcta tttataagta cgattcagtc acctattgtg
       61 aaaggtttgt ctatgtggtt agctatatta tgtctttccc tgttactaac ctttatttgt
      121 tatctcgttt ggacagtatc atatagacat agatcataca acaaagaagt tgatatcata
      181 attgtgttag gtgctggtat ttttaccgaa tttgtcacac caatgcttgc agcgcgttta
      241 gatcgtgcac ttgatatcta tcaacaacaa gcatcagcaa caaagatcat tgtatctggc
      301 ggacaaggac cagacgaacc aatttctgaa gctttagcta tgcaacgtta tttgattgca
      361 catggtgttg ataaaacaga tatactaatg gaatctcact ctataaatac atacacaaac
      421 tttttatatt ctaaacaaat catcaacaat ttgtatcatg aagcagttaa aattgtttgt
      481 gtaacaagtc agtttcatgt attgcgcgcc ttacgacttg cccaaaaatt aggtttatct
      541 atcgatggtg tcggaagtca tacaccatat cactttttct tacatgtgct catcaaagat
      601 tatctaggcg taatgtatca gtatagattg ttattaacgt tatattgttc aagtagttgg
      661 tttatatgtc tttacgccgc ttttatttat aatagttaat ccacaaacaa actattatta
      721 taggttgttt ctaatattgc gtcaaactat ctttaaatat tcgttaatcc ttgattagac
      781 aattgttata ttgaaaataa ataaactaaa gagtctggga cacattaatc cctaaaaaaa
      841 acagcagtaa gataattttc aattagaaaa tatcttactg ctgttctcta tttatacaat
      901 acttcgtatt gaatggcttc gctatgccca tctggcacat tactgtaaaa ttctataaat
      961 agaatttttg atgacgggtc ccttcctagg gtgccgtctc aaccacccca accggcacat
     1021 tgttgtaagc tgactatatg tcagcttctg tgttggggcc cctgtcttcg actggcactg
     1081 ctccctcagg agtctcgcca ttaatactac gtattaacat gtaattttac ttttaaatac
     1141 tttaaaaaaa taagacact
//
LOCUS       DAXWFK010000320         1156 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.326, whole genome shotgun sequence.
ACCESSION   DAXWFK010000320 DAXWFK010000000
VERSION     DAXWFK010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.326"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..1090
                     /gene="sasG"
                     /locus_tag="ACGEXS_002752"
     CDS             <1..1090
                     /gene="sasG"
                     /locus_tag="ACGEXS_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG-anchored surface protein SasG"
                     /protein_id="HFF8013698.1"
                     /translation="GPETITPGHRDEFDPKLPTGEKEEVPGKPGIKNPETGDVVRPPV
                     DSVTKYGPVKGDSIVEKEEIPFEKERKFNPDLAPGTEKVTREGQKGEKTITTPTLKNP
                     LTGEIISKGESKEEITKDPVNELTEFGGEKIPQGHKDIFDPNLPTDQTEKVPGKPGIK
                     NPDTGKVIEEPVDDVIKHGPKTGTPETKTVEIPFETKREFNPKLQPGEERVKQEGQPG
                     SKTITTPITVNPLTGEKVGEGQPTEEITKQPVDKIVEFGGEKPKDPKGPENPEKPSRP
                     THPSGPVNPNNPGLSKDRAKPNGPVHSMDKNDKVKKSKIAKESVANQEKKRAELPKTG
                     LESTQKGLIFSSIIGIAGLMLLARRRKN"
BASE COUNT          507 a          195 c          237 g          217 t
ORIGIN      
        1 cggaccagaa acgataacac caggtcatcg agacgaattt gatccgaagt taccaacagg
       61 agagaaagag gaagttccag gtaaaccagg aattaagaat ccagaaacag gagatgtagt
      121 tagaccaccg gtcgatagcg taacaaaata tggacctgta aaaggagact cgattgtaga
      181 aaaagaagaa attccattcg agaaagaacg taaatttaat cctgatttag caccagggac
      241 agaaaaagta acaagagaag gacaaaaagg tgagaagaca ataacgacgc caacactaaa
      301 aaatccatta actggagaaa ttattagtaa aggtgaatcg aaagaagaaa tcacaaaaga
      361 tccagttaat gaattaacag aattcggtgg cgagaaaata ccgcaaggtc ataaagatat
      421 ctttgatcca aacttaccaa cagatcaaac ggaaaaagta ccaggtaaac caggaatcaa
      481 gaatccagac acaggaaaag tgatcgaaga gccagtggat gatgtgatta aacacggacc
      541 aaaaacgggt acaccagaaa caaaaacagt agagataccg tttgaaacaa aacgtgagtt
      601 taatccaaaa ttacaacctg gtgaagagcg agtgaaacaa gaaggacaac caggaagtaa
      661 gacaatcaca acaccaatca cagtgaaccc attaacaggt gaaaaagttg gcgagggtca
      721 accaacagaa gagatcacaa aacaaccagt agataagatt gtagagttcg gtggagagaa
      781 accaaaagat ccaaaaggac ctgaaaaccc agagaagccg agcagaccaa ctcatccaag
      841 tggcccagta aatcctaaca atccaggatt atcgaaagac agagcaaaac caaatggccc
      901 agttcattca atggataaaa atgataaagt taaaaaatct aaaattgcta aagaatcagt
      961 agctaatcaa gagaaaaaac gagcagaatt accaaaaaca ggtttagaaa gcacgcaaaa
     1021 aggtttgatc tttagtagta taattggaat tgctggatta atgttattgg ctcgtagaag
     1081 aaagaattaa aataattcat aatttaaata atagttgatt tgcattcact atatttagtt
     1141 tgttaaaaac aaccta
//
LOCUS       DAXWFK010000321         1135 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.327, whole genome shotgun sequence.
ACCESSION   DAXWFK010000321 DAXWFK010000000
VERSION     DAXWFK010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.327"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            10..>1135
                     /locus_tag="ACGEXS_002753"
     CDS             10..>1135
                     /locus_tag="ACGEXS_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFF8013699.1"
                     /translation="MKLSLNSNSKYLRAPSIRQFSNRMNNLDDCVNLTIGQPDFPMPD
                     VVKKAYIDAINNDKTSYSHNKGLLETREAISQYFKNRYHFSYDPEEIIVTNGASEAID
                     TTLRSIIEPGDEIIIPGPIYAGYIPLIEVLGGKPIYIDTTATQFKITPDALESHISPK
                     TKAVLLNYPTNPTGVVLKRNEVLNIVNVLKKYPLFIISDEIYAENTFSGKHVSFAEFE
                     DIRDQLILIGGLSKSHSATGIRIGFLLGPQYLIDKLTFMHAYNCICANVPAQIACITA
                     LNEGLEAPKYMNEAYVERRNYLVSELTKLGFEITAQPEGAFYIFPSIKHITDDDFEFC
                     VDLLESTHLAIVPGSSFTEFGKGFVRISYAYEMDVLKEGMK"
BASE COUNT          403 a          181 c          187 g          364 t
ORIGIN      
        1 gtgtcacaaa tgaaactttc tttaaattct aattctaaat atttaagagc accaagtatt
       61 cgccaatttt caaatcgtat gaataatttg gatgattgtg ttaatttaac tattggccaa
      121 ccagacttcc cgatgcctga tgttgtgaaa aaggcatata ttgatgctat taacaatgac
      181 aagacatcat actctcacaa taaagggtta ttagaaactc gcgaagcaat tagtcaatac
      241 ttcaaaaatc gttatcattt ttcctatgat cctgaagaaa ttattgtgac aaatggagca
      301 agtgaagcaa tagacacaac gttacgttct atcatagagc ctggagatga aattataata
      361 ccgggaccaa tttatgcagg ctacatacca ctcatcgaag tactaggtgg taaaccaatt
      421 tatattgata caacagcaac acaatttaaa attacacctg atgcattaga aagtcatatt
      481 tctccaaaga caaaagctgt cttgttaaat tatccgacta atccaactgg cgtagtttta
      541 aaaagaaatg aagttttaaa tatcgtaaat gtattaaaaa aatatccgtt atttattatt
      601 agcgatgaga tttatgctga aaatacattt agtggtaaac atgtatcctt cgctgaattt
      661 gaagacattc gtgatcaatt aatattaatt ggtggtttaa gtaaatcaca ctcagcaacc
      721 ggaataagaa ttggttttct attaggacct caatatttga ttgataaatt aacatttatg
      781 cacgcctata attgtatttg tgccaatgtt ccagctcaaa tagcatgtat aacagcactt
      841 aacgaaggat tagaagctcc taaatatatg aacgaagctt atgtagaacg aagaaattat
      901 ttagtatctg aattaactaa attaggtttt gagattaccg ctcaacctga aggcgcgttt
      961 tatattttcc caagtattaa acatatcacc gatgacgatt tcgaattttg tgtcgattta
     1021 cttgaatcaa cacatttagc aatcgttcca ggctcatcct ttacagaatt cggtaaagga
     1081 tttgtaagga tttcctatgc ttatgaaatg gatgttttaa aagaaggtat gaaga
//
LOCUS       DAXWFK010000322         1130 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.328, whole genome shotgun sequence.
ACCESSION   DAXWFK010000322 DAXWFK010000000
VERSION     DAXWFK010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.328"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(135..1121)
                     /locus_tag="ACGEXS_002754"
     CDS             complement(135..1121)
                     /locus_tag="ACGEXS_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol-specific phospholipase C"
                     /protein_id="HFF8013700.1"
                     /translation="MKKCIKTLFLSIILVVMSGWYHSAHASDSLSKSPENWMSKLDDG
                     KHLTEINIPGSHDSGSFTLKDPVKSVWAKTQDKDYLTQMKSGVRFFDIRGRASADNMI
                     SVHHGMVYLHHELGKFLDDAKYYLSAYPNETIVMSMKKDYDSDSKVTKTFEEIFREYY
                     YNNPQYQNLFYTGSNANPTLKETKGKIVLFNRMGGTYIKSGYGADTSGIQWADNATFE
                     TKINNGSLNLKVQDEYKDYYDKKVEAVKNLLAKAKTDSNKDNVYVNFLSVASGGSAFN
                     STYNYASHINPEIAKTIKANGKARTGWLIVDYAGYTWPGYDDIVSEIIDSNK"
BASE COUNT          353 a          227 c          132 g          418 t
ORIGIN      
        1 tcaaaaattt cacaaacaat ctgttcaatt tataatattg ttgaattatt acttattttc
       61 aaaataaaaa cgagtagtga aaataattag aatctaacta ttttcatact cgtcttaata
      121 tcattattga atccttattt attactatct ataatttcac ttacgatatc atcatatcca
      181 ggccacgtat atcctgcata gtcaacaatc agccaacccg ttctagcttt cccatttgct
      241 ttaatcgttt ttgcaatttc aggatttata tgtgatgcat agttataagt actattaaat
      301 gcgctgcctc cagacgctac actcaagaaa ttcacatata cattgtcttt gttactatcc
      361 gttttagctt tagccaataa atttttaaca gcttcaactt ttttatcata gtaatcttta
      421 tactcatctt gtacttttaa atttaagcta ccattattaa ttttcgtttc aaatgtcgca
      481 ttgtctgccc attgaatacc tgacgtgtca gcaccataac cactttttat gtacgtaccc
      541 cccattctat tgaataggac aattttacct ttcgtttctt ttaaagtagg attcgcatta
      601 cttcctgtgt aaaaaagatt ctgatattgc gggttattat aataatattc tctaaaaatt
      661 tcttcaaatg tcttcgtaac tttagaatcg ctatcgtagt cctttttcat agacatcaca
      721 attgtttcgt ttggataagc actcaagtaa tatttagcat catcgagaaa ttttcctaat
      781 tcatgatgca aataaaccat gccgtgatga actgaaatca tattatcagc acttgctcta
      841 cctctaatat caaaaaacct gactcccgac ttcatttggg taaggtaatc tttatcttga
      901 gtctttgccc aaactgattt tactggatcc tttaaagtga atgagccact atcatgtgaa
      961 cccggtatat taatctcagt taaatgtttt ccatcatcaa gtttactcat ccaattttct
     1021 ggacttttac tcaacgaatc tgacgcatgt gctgaatgat accaaccact catcactact
     1081 aaaatgatac ttaaaaacaa agtcttaata cactttttca taatatcaac
//
LOCUS       DAXWFK010000323         1105 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.329, whole genome shotgun sequence.
ACCESSION   DAXWFK010000323 DAXWFK010000000
VERSION     DAXWFK010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.329"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..293)
                     /locus_tag="ACGEXS_002755"
     CDS             complement(<1..293)
                     /locus_tag="ACGEXS_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013701.1"
                     /translation="MINETKYMRQQITNLIQIIDTIKYNTLMTDWNIQTHIYKFNQIV
                     TNELNKFKGFETSYIINENQVSYYEIITLLNKRPLRQVDYGNKIQYLNFYHTEL"
     gene            342..1079
                     /gene="sdpB"
                     /locus_tag="ACGEXS_002756"
     CDS             342..1079
                     /gene="sdpB"
                     /locus_tag="ACGEXS_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic endopeptidase
                     SdpB"
                     /protein_id="HFF8013702.1"
                     /translation="MENEKKKYTFKDIAWRDLLIIPIIGVIFYALTIAAYIGPILFGY
                     YEIDSNLIFVATITQALAYTIGIGAFYLFHLKVMPSRLRAGFAYIKKYWLRLSITYII
                     ALILIYTYEYMTQFLPKHLQYSETANELELNKMFEVPAFLPVAFLLIVIVGPIVEEIV
                     FRHILIGELGKKFNFIAMSIVSVFLFAFIHVTDAKSPFEFGPYLILSIILVFTYLKSG
                     RNLGSTIALHIANNFVSFVISVISIYA"
BASE COUNT          362 a          138 c          183 g          422 t
ORIGIN      
        1 agttctgtat gataaaaatt aagatattga attttgttac catagtcgac ttgtcggagg
       61 ggacgtttat taagtagtgt tataatttca taataggaaa cttgattttc gtttattata
      121 tatgatgttt caaagccttt gaacttattc aattcattag taactatttg gttaaattta
      181 taaatatgcg tttgaatgtt ccaatctgtc attaaagtat tatatttaat cgtatcaatg
      241 atttgaatca aattagtaat ttgttgtctc atatacttag tttcgtttat catgtagaag
      301 tacctccaaa aatcaattat taaacaggaa aggatataac agtggaaaat gaaaaaaaga
      361 aatacacgtt taaagatata gcatggagag atttgcttat aattccaatt ataggtgtta
      421 tattttatgc actcacaatt gcagcttaca taggaccaat tttgtttggt tattatgaaa
      481 tcgatagtaa tctgatattt gtagcgacta tcacacaagc tttagcatat acgattggga
      541 ttggcgcatt ttatttattt catttaaaag tgatgccatc aaggcttcga gctggttttg
      601 cgtatatcaa aaaatattgg cttagattgt caataacgta tattattgct ctcatcttaa
      661 tttatacata tgaatatatg acacagtttc tacctaaaca tttgcaatat agtgaaacag
      721 ccaatgaact tgagctaaat aaaatgtttg aagtgcctgc atttttacct gtagcattct
      781 tattaattgt gattgtaggt ccaatagttg aagaaatcgt atttagacat attttaattg
      841 gagaattagg taagaagttt aattttatag ctatgagtat tgtttcggtg ttcttatttg
      901 catttattca tgtgactgat gcaaagtcgc cgtttgagtt tggtccatat ctcatactat
      961 ccatcatatt agttttcact tatttaaaat ctggaagaaa cctaggttca acaattgcat
     1021 tacatatagc aaataatttt gtttctttcg ttatatcagt aataagtatt tatgcgtaga
     1081 taaagaggtc gggacattaa gtttt
//
LOCUS       DAXWFK010000324         1100 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.330, whole genome shotgun sequence.
ACCESSION   DAXWFK010000324 DAXWFK010000000
VERSION     DAXWFK010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.330"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            59..1018
                     /gene="hyl"
                     /locus_tag="ACGEXS_002757"
     CDS             59..1018
                     /gene="hyl"
                     /locus_tag="ACGEXS_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hemolysin"
                     /protein_id="HFF8013703.1"
                     /translation="MKTRIVSSVTTTLLLGSILMNPVANAADSDINIKTGTTDIGSNT
                     TVKTGDLVTYDKENGMHKKVFYSFIDDKNHNKKLLVIRTKGTIAGQYRVYSEEGANKS
                     GLAWPSAFKVQLQLPDNEVAQISDYYPRNSIDTKEYMSTLTYGFNGNVTGDDTGKIGG
                     LIGANVSIGHTLKYVQPDFKTILESPTDKKVGWKVIFNNMVNQNWGPYDRDSWNPVYG
                     NQLFMKTRNGSMKAADNFLDPNKASSLLSSGFSPDFATVITMDRKASKQQTNIDVIYE
                     RVRDDYQLHWTSTNWKGTNTKDKWIDRSSERYKIDWEKEEMTN"
BASE COUNT          418 a          164 c          196 g          322 t
ORIGIN      
        1 attgacatat tgattattgt ttcctcaaat cgttttaaaa aatagaagga tgatgaaaat
       61 gaaaacacgt atagtcagct cagtaacaac aacactattg ctaggttcca tattaatgaa
      121 tcctgtcgct aatgccgcag attctgatat taatattaaa accggtacta cagatattgg
      181 aagcaatact acagtaaaaa caggtgattt agtcacttat gataaagaaa atggcatgca
      241 caaaaaagta ttttatagtt ttatcgatga taaaaatcat aataaaaaac tgctagttat
      301 tagaacgaaa ggtaccattg ctggtcaata tagagtttat agcgaagaag gtgctaacaa
      361 aagtggttta gcctggcctt cagcctttaa ggtacagttg caactacctg ataatgaagt
      421 agctcaaata tctgattact atccaagaaa ttcgattgat acaaaagagt atatgagtac
      481 tttaacttat ggattcaacg gtaatgttac tggtgatgat acaggaaaaa ttggcggcct
      541 tattggtgca aatgtttcga ttggtcatac actgaaatat gttcaacctg atttcaaaac
      601 aattttagag agcccaactg ataaaaaagt aggctggaaa gtgatattta acaatatggt
      661 gaatcaaaat tggggaccat atgatagaga ttcttggaac ccggtatatg gcaatcaact
      721 tttcatgaaa actagaaatg gctctatgaa agcagcagat aacttccttg atcctaacaa
      781 agcaagttct ctattatctt cagggttttc accagacttc gctacagtta ttactatgga
      841 tagaaaagca tccaaacaac aaacaaatat agatgtaata tacgaacgag ttcgtgatga
      901 ctaccaattg cactggactt caacaaattg gaaaggtacc aatactaaag ataaatggat
      961 agatcgttct tcagaaagat ataaaatcga ttgggaaaaa gaagaaatga caaattaatg
     1021 taaattattt gttcatgtac aaataaatat aatttataac tttagccgat aacttcagaa
     1081 atgatgtttt tcggctaatt
//
LOCUS       DAXWFK010000325         1100 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.331, whole genome shotgun sequence.
ACCESSION   DAXWFK010000325 DAXWFK010000000
VERSION     DAXWFK010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.331"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            13..960
                     /locus_tag="ACGEXS_002758"
     CDS             13..960
                     /locus_tag="ACGEXS_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFF8013704.1"
                     /translation="MKRSSKSKMSLPKKIFLWVFGILVILAIVAVVYVAAKIFITGNK
                     IHNPLDRNHSELRDKKVSLNDGDPFTIALFGVDSDADRKKKGGGERSDSIMILSINPK
                     TKKTEIVSIPRDTRAEIVGRGTTEKIAHAYAYGGPNMAVKSLEKLMNVPIDHYATIDM
                     DGLHNMIDSIGGVDVVSNDTFTVDGVRFTKGQQTHVNGDQALKFIRSRKEEGAGGDFG
                     RQQRQQIVLEAMANKIASPSSITHFNSLMNEIQNNVKTDLTLGDLNTIRSNYKDANDT
                     INKHQLSGQGGIQSDGLYYFIPSEQSKAESTKLLKDNLE"
BASE COUNT          397 a          142 c          235 g          326 t
ORIGIN      
        1 ggagtggtgg gattgaaacg ttcgtctaaa agtaaaatga gcctaccgaa aaaaatattt
       61 ttatgggtat tcggtatttt agtaatctta gcgattgtag ctgttgtgta tgtagctgct
      121 aaaattttta ttactggtaa taagatacac aatccgttag atcgcaatca ttcagaatta
      181 agagataaaa aagtgagttt aaacgatggt gatccattca caatcgcttt atttggtgtc
      241 gattcagatg ctgatcgtaa gaaaaagggt ggcggagagc gtagtgatag tattatgatt
      301 ttatctatca accctaaaac gaagaaaact gaaatagtaa gtataccgcg tgatacaaga
      361 gcggaaattg ttggccgtgg tactactgaa aaaattgcac atgcctatgc atatggtgga
      421 ccaaatatgg ctgttaaatc acttgaaaaa ttaatgaatg tgccaattga tcattatgcg
      481 acaatcgata tggatggttt gcataatatg atagatagca tcggcggtgt tgatgtagta
      541 agtaatgata cattcactgt tgatggtgtt cgttttacaa aaggacagca gacacatgta
      601 aatggtgatc aagcattgaa gtttattaga agtcgtaaag aagaaggtgc tggtggtgac
      661 tttggtcgac aacaacgtca gcaaattgtg cttgaagcga tggccaataa gattgcaagc
      721 ccatcatcga ttacacattt taatagtcta atgaatgaaa ttcaaaataa tgttaaaaca
      781 gatttaacat taggtgattt gaatacgatt agaagcaatt ataaagatgc taatgacacg
      841 attaataaac atcaattgag tggtcaaggc ggcattcaaa gtgacggatt gtactacttc
      901 attcctagtg agcagtcaaa agctgaaagt actaagttat taaaagataa tctagagtag
      961 taaactaagg gaaaataagt tatcaaaatt gtacacaaat taattagata gaatgatctg
     1021 taaatagaat aacgaataga actaatagcc cgtaaataga aggtgataac actttgtatt
     1081 tatgggctat tttatttaat
//
LOCUS       DAXWFK010000326         1097 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.332, whole genome shotgun sequence.
ACCESSION   DAXWFK010000326 DAXWFK010000000
VERSION     DAXWFK010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.332"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            65..862
                     /locus_tag="ACGEXS_002759"
     CDS             65..862
                     /locus_tag="ACGEXS_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFF8013705.1"
                     /translation="MKKLAFAITATSGAAAFLTHHDAQASTQHTVQSGESLWSIAQKY
                     NTSVESIKQNNQLDNNLVFPGQVISVGGSDAQNTSNTSPQAGSASSHTVQAGESLNII
                     ASRYGVSVDQLMAANNLRGYLIMPNQTLQIPNGGSGGTTPTATTGSNGNASSFNHQNL
                     YTAGQCTWYVFDRRAQAGSPISTYWSDAKYWAGNAANDGYQVNNTPSVGSIMQSTPGP
                     YGHVAYVERVNGDGSILISEMNYTYGPYNMNYRTIPASEVSSYAFIH"
BASE COUNT          382 a          194 c          175 g          346 t
ORIGIN      
        1 tactatataa attgtcttaa taatttttaa aagtagtaaa acataatttt aaggaggagt
       61 ccctttgaaa aaattagcat ttgcaataac agcaacatct ggtgcagctg catttttaac
      121 gcatcatgat gcacaagctt ctacacaaca tacagtacaa tctggtgaat cattatggag
      181 tattgctcaa aaatacaaca cttcagtaga gagtattaaa caaaataacc aattagataa
      241 caacttggta ttccctggtc aagttatctc agtaggtgga agtgatgcac aaaatacgtc
      301 aaacacttct ccacaagctg gttcagcatc atctcatact gtacaagctg gtgaatcatt
      361 aaatatcatt gctagcagat atggtgtttc agttgatcaa ttaatggcag ccaataactt
      421 acgtggttat ttaattatgc ctaaccaaac attacaaatt cctaatggtg gatcaggtgg
      481 tacaacacca acagctacaa caggtagcaa tggcaatgca tcatctttta atcaccaaaa
      541 tttatacact gctggtcaat gtacatggta cgtatttgac cgtcgtgctc aagctggtag
      601 tccaattagc acatattggt cagacgctaa gtattgggct ggtaacgcag ctaatgatgg
      661 ttaccaagta aacaacacac catcagttgg ttcaattatg caaagcacac ctggtccata
      721 tggtcatgtt gcttatgttg aacgtgtcaa tggtgatggt agtatcttga tttctgaaat
      781 gaattacaca tatggtccat acaatatgaa ctaccgtaca attccagctt cagaagtttc
      841 tagctatgca ttcatccatt aattaaataa attgtactga tatatactag caattcacat
      901 catgtgagat tgctagtttt ttatttttga aaaaaatttt cattttggta caaaaaatta
      961 tctcaccctt ccctatcata catatttata ttttgtatga atggtagtta ggtaaaaatt
     1021 aacaacctac ctatttgatt aaattaaatg cataatatat taaaaatcaa aataatgcaa
     1081 ctaagttgac tatataa
//
LOCUS       DAXWFK010000327         1073 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.333, whole genome shotgun sequence.
ACCESSION   DAXWFK010000327 DAXWFK010000000
VERSION     DAXWFK010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.333"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..685
                     /locus_tag="ACGEXS_002760"
     CDS             <1..685
                     /locus_tag="ACGEXS_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013706.1"
                     /translation="SQEAQEVQEAKKEAQAEKDSDTLTKDASAAKVEVSKPESQAERL
                     ANAAKQKQAKLTPGSKESQLTEALFAEKPVAKNDLKEIPQLVTKKNDVSETETVNIDN
                     KDTVKQKEAKFENGVITRKADEKTTNNTAVDKKSGKQSKKTTPSNKRNASKASTNKTS
                     GQKKQHNKKSSQGAKKQSSSSKSTQKNNQTSNKNSKTTNAKSSNASKTPNAKVEKAKS
                     KIEKRTFND"
     gene            820..924
                     /locus_tag="ACGEXS_002761"
     CDS             820..924
                     /locus_tag="ACGEXS_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013707.1"
                     /translation="MRSFHVFMLFETSLDNNSDIAHFKLKMEKYSIIK"
BASE COUNT          479 a          145 c          178 g          271 t
ORIGIN      
        1 atcacaagag gcacaagagg ttcaagaagc taaaaaagaa gcacaagcag agaaagacag
       61 tgacacatta actaaagatg caagtgcagc aaaggtagaa gtatcaaaac cagagtcaca
      121 agctgaaaga ttagcaaacg ctgcaaaaca gaagcaagct aaattaacac caggttcaaa
      181 agagagtcaa ttaactgaag cgttatttgc agaaaaacca gttgctaaaa atgacttgaa
      241 agaaattcct caattagtta ctaaaaagaa tgatgtatca gagacagaga cggttaatat
      301 agataataaa gacactgtta aacaaaaaga agctaaattt gaaaatggtg ttattacacg
      361 taaagctgat gaaaaaacaa ctaataatac agctgttgac aagaaatcag gtaaacaatc
      421 taaaaaaaca acaccttcaa ataaacgaaa tgcatcaaaa gcatctacaa ataaaacttc
      481 aggtcagaaa aagcaacata ataagaaatc atcacaaggt gcaaagaaac aaagtagttc
      541 aagtaagtca actcaaaaga ataatcaaac tagtaataag aattcaaaaa caacaaatgc
      601 taaatcatcc aatgcatcaa aaacgccaaa tgctaaagtt gagaaagcta aaagtaaaat
      661 agagaaacgt acattcaatg actaagatta ctaaaatcgt tgaataatca atcatagcag
      721 taagctatgt aatatatttt taaaaattga atttcaaaat aaaaggtagg cattcaggtg
      781 cttacctttt ttgatatcat cagtttattt tgaaaggcaa tgcgatcatt tcatgtattt
      841 atgttgtttg aaacatcgtt agataacaat agtgatattg cacattttaa gctgaagatg
      901 gaaaaatatt cgataatcaa ataaacaatg aattttagaa ggtacaatga cgttcactaa
      961 tttaaatata gctgaatgtg ttggtgagtg atgttcacta tagatttata ttaatataca
     1021 aagacaaagg ttgttaattt ttattaagcg ttaggttgaa tgtatgagaa ttt
//
LOCUS       DAXWFK010000328         1067 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.334, whole genome shotgun sequence.
ACCESSION   DAXWFK010000328 DAXWFK010000000
VERSION     DAXWFK010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.334"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..862)
                     /locus_tag="ACGEXS_002762"
     CDS             complement(<1..862)
                     /locus_tag="ACGEXS_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HFF8013708.1"
                     /translation="MEEFKQHYKGLIDESLTCQDKVELIKKCEKYTDEVIRKDVLPED
                     IVDIHKNYILTLNLTREDVFKTLDVLQEIVKGFGYSYRDYQRLVDKLQVHDKEIDLAS
                     SLQQTMLKTDIPQFDSIQIGVISVAAQKVSGDYFNLIDHNDGTMSFAVADVIGKGIPA
                     ALAMSMIKFGMDSYGHSQLPSDGLKRLNRVVEKNINQNMFVTMFYGLYEEMNHLLYCS
                     SAGHEPGYIYRAEKEEFEEISVRGRVLGISSQTRYQQQEIPIYLDDLIIILTDGVTEA
                     RNSEGTFIDKQ"
BASE COUNT          339 a          208 c          135 g          385 t
ORIGIN      
        1 tttgtttatc tataaaggta ccttcactat ttctagcttc agtcacacca tccgttaaaa
       61 tgataattaa atcatcaagg tatattggaa tttcttgttg ttgatatcgt gtttgtgaac
      121 tgattcctaa cactctacct ctaactgaaa tttcttcaaa ttcttctttt tcagcgcgat
      181 aaatatatcc aggctcatga ccagctgaac tacaatacaa taaatggttc atttcttcat
      241 ataaaccata aaacattgtg acgaacatat tttgattaat attcttttca acaacacgat
      301 ttaaacgttt taaaccatca ctcggtaatt gtgagtgtcc ataagaatcc atgccaaact
      361 ttatcatact cattgctaaa gcagctggta taccttttcc aatgacatct gcaacagcaa
      421 agctcattgt gccatcgtta tggtcaatta aattaaaata atctccactt actttttgtg
      481 ccgccactga aataacgcca atttgaatac tatcaaattg tggaatatct gttttaagca
      541 ttgtttgttg taagctagaa gctaagtcta tctctttatc gtgaacttga agtttatcta
      601 ccaatctttg ataatctcga taactataac caaagccttt aacgatttct tgtaagacat
      661 ctaatgtctt gaacacatct tcacgcgtta agtttaacgt caatatatag tttttgtgaa
      721 tatcgacaat gtcttcaggc aagacgtcct tacgaatcac ttcgtcagtg tatttctcac
      781 acttttttat caattctact ttatcttggc acgttaaact ttcatcaatt aaacccttat
      841 aatgttgctt aaattcttcc acgattagtt gcctcctcac ttcccttaca aagcaaatgc
      901 ttaagcaagg ataattatga ttcacttact aacatttatt taattgcact tatcaatttt
      961 gtcgtttgca attttgctgt agatgatact tttaagctaa aaaaacttta ggatggaata
     1021 cctaaagcct aaaaaactta agacatcctt ttataataaa aaatatg
//
LOCUS       DAXWFK010000329         1066 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.335, whole genome shotgun sequence.
ACCESSION   DAXWFK010000329 DAXWFK010000000
VERSION     DAXWFK010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.335"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..292
                     /gene="lpl8"
                     /locus_tag="ACGEXS_002763"
     CDS             <1..292
                     /gene="lpl8"
                     /locus_tag="ACGEXS_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl8"
                     /protein_id="HFF8013709.1"
                     /translation="LKNYNNGDVSINENVPSYDAKFKMSNKDENVKQLRSRYNIPTDK
                     APVLKMHIDGNLKGSSVGYKKLEIDFSKGGKSDLSVIDSLNFQPAKVDEDDE"
     gene            311..>1066
                     /gene="lpl9"
                     /locus_tag="ACGEXS_002764"
     CDS             311..>1066
                     /gene="lpl9"
                     /locus_tag="ACGEXS_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl9"
                     /protein_id="HFF8013710.1"
                     /translation="MKSIKRIGLCISLLILIIFVTSCDGDNKIIGDSKEEQIKKSFAK
                     TLDIYPIKNLEDLYDKEGYRDGEFKKDDKGTWLIRSEMKIQLKGENLESRGAVLEINR
                     NTRTAKGHYIVREVVEDSDGMTHNHTKRYPVKMENNKMIPLKPIDDEKVKKEIEEFNF
                     FVQYGNFKELENYKEDEVSYNPEVPIYSAKYQLKNSDYNVEQLRKRYNIPTQKAPKLL
                     LKGSGNLKGSSVGYKNIEFTFIENKEENIYFTDS"
BASE COUNT          442 a          123 c          209 g          292 t
ORIGIN      
        1 attgaaaaac tacaacaatg gtgatgtctc aattaatgag aatgttccaa gttatgacgc
       61 aaaatttaaa atgagcaata aagatgaaaa tgttaagcaa ttaagaagtc gttataatat
      121 tcctactgat aaagcaccgg tattaaaaat gcatattgat ggtaatttga aaggaagttc
      181 tgtgggttat aaaaagttgg aaattgactt ttcaaaaggt ggaaaaagcg atttgtcagt
      241 aatagattct ttgaatttcc agccggcgaa ggtagatgaa gatgatgaat gatgaggatg
      301 gtgtgtaaca atgaagtcta taaaaaggat tggattgtgc attagtttgt tgattttaat
      361 catctttgtt acatcttgtg atggtgataa taagatcatt ggagattcaa aagaagaaca
      421 aatcaaaaag agctttgcga aaacgttaga tatctaccct attaagaatc tcgaggattt
      481 atacgacaaa gaaggatatc gagatggcga atttaaaaaa gatgataaag gtacttggct
      541 aattagatct gaaatgaaaa tccaattaaa aggagaaaat ctggaatcta gaggagcagt
      601 tttagaaatt aacagaaata ctagaacggc taaagggcat tatattgtta gagaagttgt
      661 tgaagatagc gatggaatga cacacaatca tacaaaaaga tatcctgtga aaatggaaaa
      721 taataaaatg attccattaa aaccaatcga tgacgaaaaa gtaaaaaaag aaatcgaaga
      781 atttaacttc tttgtacaat acgggaattt caaagaattg gaaaactata aagaggacga
      841 agtgtcatat aacccagaag taccaattta ctctgcaaaa tatcaattga aaaacagtga
      901 ttacaatgtt gaacaattaa ggaagcgata taatatcccg acgcaaaaag cgcccaaatt
      961 attattgaaa ggctcaggta atttaaaagg ttcatcagtc ggatataaaa atattgaatt
     1021 tacctttatt gaaaacaagg aagaaaatat ttacttcaca gatagt
//
LOCUS       DAXWFK010000330         1036 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.336, whole genome shotgun sequence.
ACCESSION   DAXWFK010000330 DAXWFK010000000
VERSION     DAXWFK010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.336"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(135..223)
                     /locus_tag="ACGEXS_002765"
     tRNA            complement(135..223)
                     /locus_tag="ACGEXS_002765"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(186..188),aa:Ser,seq:gct)
     gene            complement(226..300)
                     /locus_tag="ACGEXS_002766"
     tRNA            complement(226..300)
                     /locus_tag="ACGEXS_002766"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(266..268),aa:Asn,seq:gtt)
     gene            complement(442..1011)
                     /locus_tag="ACGEXS_002767"
     CDS             complement(442..1011)
                     /locus_tag="ACGEXS_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HFF8013711.1"
                     /translation="MYSQNIYVIRKGDMVIRPAFDDDDQRNGSEIIRFDKTRIQNPFK
                     VQKIIERSCKFYGNTYLGKKAETNRITGISSKPPILLTPLFPTYFFPTHSDRQNENIW
                     LNMHYIESIKELKNRKCKVTFINNESIILHVSYHSLWHQYNNSIFYYYMVDKQSRMIS
                     KNPDQPIDYNKATLNVFEALTRYSLFEDK"
BASE COUNT          349 a          164 c          171 g          352 t
ORIGIN      
        1 acctttctta gagaggatta tctaggaaag gtttttagaa ttttaaaatt gatataaaag
       61 aacagtgaaa ttctcattca ttatatataa gtaaacaaaa aagctaaaat ggtatatacc
      121 attttagcaa gtaacggaaa cggagggatt cgaaccctcg cgccgctttc gcgacctaca
      181 cccttagcag gggcgcctct tcagccaact tgagtacgtt tccaatggct ccacaggtag
      241 gactcgaacc tacgaccgat cggttaacag ccgatagctc taccactgag ctactgtgga
      301 ttaatttgta tgtgtttgaa tatcaagcac aaatattatt ataacaagat taagttaatt
      361 tgcaatagat accttaaaaa aatatgcaga acatttacac tatatttata aaacattccg
      421 catattttaa aaataaacaa tttatttatc ttcaaataaa gaatagcgtg tcaatgcttc
      481 aaacacattc aatgtggctt tattataatc tattggttgg tcgggatttt ttgatatcat
      541 gcgagattgt ttatctacca tgtaatagta aaaaatggaa ttgttatatt gatgccataa
      601 actgtggtat gaaacatgaa gaatgattga ttcattatta ataaatgtca ctttacattt
      661 acgatttttt aattctttaa tactttcgat ataatgcata tttaaccaaa tattttcatt
      721 ttgtctgtca gaatgtgttg ggaaaaaata agttggaaat aatggtgtta gtaaaatagg
      781 tggtttacta gaaatgccag taatgcggtt tgtctctgct ttcttgccaa gataagtatt
      841 accataaaat ttgcaagagc gttcaatgat tttctggact ttaaaaggat tttgaatacg
      901 cgttttgtca aaccgaatta tttcactacc gtttctttga tcgtcatcat caaatgctgg
      961 tcgaataacc atgtctcctt tgcgtatcac ataaatattt tgagaataca taagcaaaac
     1021 ctcgctttat taagtt
//
LOCUS       DAXWFK010000331         1033 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.337, whole genome shotgun sequence.
ACCESSION   DAXWFK010000331 DAXWFK010000000
VERSION     DAXWFK010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.337"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..>1033
                     /locus_tag="ACGEXS_002768"
     CDS             <1..>1033
                     /locus_tag="ACGEXS_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HFF8013712.1"
                     /translation="SKKCTYWVTLIITQILLTTSVLWILNYTGNLDLFILRIVLLSCL
                     FILTISVINLWTFLMLLNLNIANMTKGKQHFKTIRFINTVCKSILLVLIANVMIENTS
                     VIKDLNKIKETEKYWNVLDDYYTIEFAPYHETKQSLIDNMVRSEQLVKASEAENNAIL
                     FKPKGDSVDNDNFSPDEGNVILVNNQFWSIYYKQFQPDIPIKNQKNNVEVIIPQKFHA
                     MRNEINQAYHSWFEFVQNKNNKENKLSIQFINKNYCRIFSFDARDSRHLSFIEAPIIV
                     NVQASDLSNDFYYAMISQGGYLFKNYDALVKNIEKYHLDGEISGITNYKDSVMEMYHE
                     NNLKLTVLNF"
BASE COUNT          392 a          129 c          167 g          345 t
ORIGIN      
        1 tcaaagaaat gcacgtattg ggtaacgtta atcataactc aaattctttt aactacaagt
       61 gtactttgga tattaaatta cacgggtaat ttagatttat tcatattaag aatagttctg
      121 ctttcatgtc ttttcatttt aacgattagt gttattaatt tatggacttt cttaatgtta
      181 ctaaatttaa atattgcaaa tatgactaaa ggtaagcagc actttaaaac aattcgtttt
      241 attaatacag tttgtaaaag tattctctta gtactaatag ctaatgtaat gatagaaaat
      301 actagtgtta ttaaagattt gaataaaata aaagaaactg agaaatattg gaatgtatta
      361 gatgattatt acacgattga atttgcacct tatcacgaaa caaaacaaag tttgattgat
      421 aatatggtgc gatcagaaca attagtaaag gctagtgaag cagaaaataa tgcgatttta
      481 ttcaaaccaa agggtgactc cgttgacaat gacaactttt cgcctgatga ggggaatgta
      541 atattagtaa ataatcagtt ctggtcgatt tattacaagc agtttcaacc tgatattccg
      601 ataaaaaatc aaaaaaataa tgtcgaagta attattccac aaaagtttca tgcaatgcgt
      661 aatgaaatca atcaagcata tcattcatgg tttgaatttg tacaaaataa aaataataaa
      721 gagaataagt tatctataca gtttatcaac aaaaattatt gtcgaatttt ttcatttgat
      781 gcacgagata gtcgccattt gtcatttata gaggcgccaa tcattgtgaa tgttcaggca
      841 tcagatttat cgaatgattt ttattatgcc atgatcagtc aaggcgggta tttattcaaa
      901 aattatgacg cgctagtaaa aaatattgaa aagtatcatc ttgatgggga aatcagtgga
      961 ataaccaatt ataaagatag cgtgatggaa atgtatcatg aaaacaattt gaaattaaca
     1021 gtactcaact ttt
//
LOCUS       DAXWFK010000332         1028 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.338, whole genome shotgun sequence.
ACCESSION   DAXWFK010000332 DAXWFK010000000
VERSION     DAXWFK010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.338"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..584
                     /locus_tag="ACGEXS_002769"
     CDS             <1..584
                     /locus_tag="ACGEXS_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HFF8013713.1"
                     /translation="FGKFGKGNMTVLQEYDAFNTTIVSKQEIENEKISTTSIRQDLIN
                     GELQKANDALGYIYSIKGTVVQGEKRGRTIGFPTANIQPSDDYLLPRKGVYAVSIEIG
                     TENKLYRGVANIGVKPTFHDPNKAEVVIEVNIFDFEDNIYGERVTVNWHHFLRPEIKF
                     DGIDPLVKQMNDDKSRAKYLLAVDFGDEVAYNI"
     gene            699..968
                     /gene="rpsO"
                     /locus_tag="ACGEXS_002770"
     CDS             699..968
                     /gene="rpsO"
                     /locus_tag="ACGEXS_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFF8013714.1"
                     /translation="MAISQERKNEIIKEYRVHETDTGSPEVQIAVLTAEINAVNEHLR
                     THKKDHHSRRGLLKMVGRRRHLLNYLRSKDIQRYRELIKSLGIRR"
BASE COUNT          362 a          158 c          191 g          317 t
ORIGIN      
        1 cttttggtaa atttggaaaa ggtaatatga ctgtacttca agaatatgat gcgtttaata
       61 cgacaattgt gagtaaacaa gaaattgaaa atgaaaaaat ttctacaact tctattcgtc
      121 aagatttaat caatggtgag ttgcaaaaag cgaatgatgc tttaggctat atatattcta
      181 ttaaaggcac tgtagtgcaa ggtgaaaaaa ggggaagaac tattggcttc ccaacagcta
      241 acattcaacc tagtgatgat tatttgttac ctcgtaaagg tgtttatgct gttagtattg
      301 aaatcggcac tgaaaataaa ttatatcgag gggtagctaa cataggtgta aagccaacat
      361 ttcatgatcc taacaaagca gaagttgtca tcgaagtgaa tatctttgac tttgaggata
      421 atatttatgg tgaacgagtg accgtgaatt ggcatcattt cttacgtcct gagattaaat
      481 ttgatggtat cgacccatta gttaaacaaa tgaacgatga taaatcgcgt gctaaatatt
      541 tattagcagt tgattttggt gatgaagtag cttataatat ctagagttgc gtatagttat
      601 ataaacaatc tataccacac ctttttctta gtaggtcgaa tctccaacgc ctaactcgga
      661 ttaaggagta ttcaaacatt ttaaggagga aattgattat ggcaatttca caagaacgta
      721 aaaacgaaat cattaaagaa taccgtgtac acgaaactga tactggttca ccagaagtac
      781 aaatcgctgt acttactgca gaaatcaacg cagtaaacga acacttacgt acacacaaaa
      841 aagaccacca ttcacgtcgt ggattattaa aaatggtagg tcgtcgtaga catttattaa
      901 actacttacg tagtaaagat attcaacgtt accgtgaatt aattaaatca cttggtatcc
      961 gtcgttaatc ttaatataac gtctttgagg ttggggcata tttatgttcc aaccttaatt
     1021 tatattaa
//
LOCUS       DAXWFK010000333         1025 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.339, whole genome shotgun sequence.
ACCESSION   DAXWFK010000333 DAXWFK010000000
VERSION     DAXWFK010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.339"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..>1025
                     /locus_tag="ACGEXS_002771"
     CDS             <1..>1025
                     /locus_tag="ACGEXS_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8013715.1"
                     /translation="NLEQSVNNYLELEEEFEEKKKYLSEIYELIDEINIDPYIFLTEH
                     NECNIYKISENEKLELQTYMSKIEKVLLKYQTYLKETCKECLFYPYLLVQGEAGIGKS
                     HLLAHLSKKLRDENHIIYLFLGQFFTKNEDPWHQILNDLEVTNSVDNFLRSISNKAKE
                     TKKRAFIIIDALNEGEGKRLWGNYFQSFINHIKKYSNIALIFSIRTPFEDVILPKNAI
                     QDNNIVVFQHEGFSKEENYNPIVSFCDFYGLELPKLPILNPEFNNPLFLKLMCEYCVN
                     KFKEFDQTISVAELFTNVLKTVNINLSKEDKFDFDKNINVVQKVIKGLVELMNDSEFN
                     QLNYEES"
BASE COUNT          420 a          109 c          153 g          343 t
ORIGIN      
        1 taatttagaa caatcagtca ataattattt ggaactggaa gaagagtttg aagaaaagaa
       61 aaaatactta agtgaaattt atgaattgat agacgaaatt aatattgacc cttatatttt
      121 tctgacagaa cataatgaat gtaatatata caaaataagt gagaatgaaa aattagaact
      181 tcaaacttat atgagtaaaa tcgaaaaagt attattaaaa tatcaaacgt atttaaaaga
      241 aacatgtaaa gaatgcttgt tttatcctta tttgctagtt caaggtgaag ctggcatagg
      301 aaaatcacac cttttagcgc atctatctaa aaaacttaga gatgaaaatc atattattta
      361 tctgtttttg ggacaatttt ttactaaaaa tgaagatcca tggcatcaaa tacttaatga
      421 tttagaagtt acaaattctg ttgataattt tttaaggtca ataagtaata aggccaaaga
      481 aacaaaaaaa agagctttta ttattattga tgcgcttaat gaaggtgaag gtaaaaggtt
      541 atggggaaat tattttcaaa gctttatcaa ccatataaag aagtattcta atatagcttt
      601 aatattttct attagaaccc cttttgaaga tgttatatta cccaaaaacg caatacaaga
      661 taacaatatt gtagtatttc agcatgaagg ttttagtaag gaagaaaact ataatccaat
      721 tgtatctttt tgtgattttt atggattaga gctacctaag ttacctatat taaatccaga
      781 attcaacaat ccattatttt taaaactaat gtgtgagtac tgtgtaaata aatttaagga
      841 atttgaccaa actataagtg ttgcagagct atttacaaat gttcttaaga cagtaaatat
      901 taatttatct aaggaagata aatttgattt tgataaaaat attaatgttg tacaaaaagt
      961 aataaaagga ttagttgaat taatgaacga ctcagaattt aatcaactaa attatgagga
     1021 atcct
//
LOCUS       DAXWFK010000334         1024 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.340, whole genome shotgun sequence.
ACCESSION   DAXWFK010000334 DAXWFK010000000
VERSION     DAXWFK010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.340"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..310
                     /locus_tag="ACGEXS_002772"
     CDS             <1..310
                     /locus_tag="ACGEXS_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HFF8013716.1"
                     /translation="IYYKEQQARITLYLKHNTKEPNTTKSVHFTNSETSPMGSAVIEG
                     YINENKKDNFTAYATPEHNYQFGGAMIESEKLSELLKPANQLKSPDDIKKELNKKKSH
                     "
     gene            328..747
                     /locus_tag="ACGEXS_002773"
     CDS             328..747
                     /locus_tag="ACGEXS_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HFF8013717.1"
                     /translation="MSKKHVFIIIGVILCICTVSTVMHFKMKYDEKEKQKAIYYKEQQ
                     ERITLYLKHNTKETNTIKSVHFTNLETSPMGSAVIEGYINENKEDDFTAYASPEHNYQ
                     FGGAMIKSEGVDKLLKPAHERKSPEKIKEELDKKEGH"
BASE COUNT          422 a          153 c          160 g          289 t
ORIGIN      
        1 aatatactat aaagaacaac aagcgcgcat tacactttat ctcaagcata acactaaaga
       61 accgaataca accaaatctg tacatttcac aaactcggaa acaagtccta tgggaagtgc
      121 tgtgattgaa ggttacataa atgaaaacaa aaaagataat tttactgctt atgctacacc
      181 agaacataat tatcaatttg gtggtgctat gatagaaagt gaaaaattaa gcgagttact
      241 aaagccagcc aatcagttaa aatcaccaga tgatataaaa aaagaactaa ataaaaagaa
      301 gagtcactaa agttaggagt tactttaatg tccaaaaaac atgtttttat aattattggt
      361 gtcatattgt gtatatgtac agtttctacg gtcatgcatt ttaaaatgaa atatgatgaa
      421 aaagaaaaac aaaaagcgat ttactacaaa gaacaacaag aacgtattac actctatctt
      481 aagcataata ctaaagaaac gaacacgatt aaatctgtac atttcacaaa cttggaaaca
      541 agtcctatgg gaagtgctgt gattgaagga tacatcaatg aaaataaaga agatgatttt
      601 actgcttatg catcgcctga acataattat caatttggtg gcgctatgat aaaaagtgaa
      661 ggagtagata aattattaaa accagcacat gaaagaaaat caccagaaaa aatcaaagaa
      721 gaattagata aaaaagaagg ccactagggt cttctttatt tttgatttaa tcttccaata
      781 atctatgtca ttgctatcga aggtgtttcg caattaatat aaatcacttc atcatcacca
      841 atacttcccc agttttgtac agtacattaa cacaaacgaa ccacgttaat ttaaatggaa
      901 taatagtttg gccattataa gaacaatata tatcgaataa caatgtcgat gacgtgattt
      961 attgacatta tcaaattttc tcaaatgtgt gtgatttttt ataaaaaagg gtagagcaag
     1021 gtat
//
LOCUS       DAXWFK010000335         1010 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.341, whole genome shotgun sequence.
ACCESSION   DAXWFK010000335 DAXWFK010000000
VERSION     DAXWFK010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.341"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..721
                     /gene="sarS"
                     /locus_tag="ACGEXS_002774"
     CDS             <1..721
                     /gene="sarS"
                     /locus_tag="ACGEXS_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarS"
                     /protein_id="HFF8013718.1"
                     /translation="FIIIEAYMFRFKKKVKPEVDMTIKEFILLTYLFHQQENTLPFKK
                     IVSDLCYKQSDLVQHIKVLVKHSYISKVRSKIDERNTYISISEEQREKIAERVTLFDQ
                     IIKQFNLADQSESQMIPKDSKEFLNLMMYTMYFKNIIKKHLTLSFVEFTILAIITSQN
                     KNIVLLKDLIETIHHKYPQTVRALNNLKKQGYLIKERSTEDERKILIHMDDAQQDHAE
                     QLLAQVNQLLADKDHLHLVFE"
BASE COUNT          385 a          158 c          122 g          345 t
ORIGIN      
        1 ttttataatc attgaagcat atatgtttcg ttttaagaaa aaagtcaagc ctgaagtcga
       61 tatgactata aaagaattta tattactgac ttatttattt catcagcaag aaaacacact
      121 tccatttaag aagattgttt cagatttatg ttataaacaa tcggatttag tacagcatat
      181 aaaagtactt gtgaaacatt catatattag taaagttcga agtaaaattg atgagcgtaa
      241 tacttacatt tcaatatctg aagaacaacg agagaaaatt gcagaacgtg ttacattgtt
      301 tgatcaaatc attaaacaat ttaaccttgc agatcaaagt gaatcacaga tgataccaaa
      361 agatagtaaa gaatttctaa acttgatgat gtatacaatg tatttcaaga atattatcaa
      421 aaaacatcta acattaagtt ttgtagaatt cacaattcta gctattatca cttctcaaaa
      481 taaaaacatc gttcttctta aagatttaat tgaaacaatc caccataaat accctcaaac
      541 tgttagagct ctcaataatt taaaaaagca aggctatcta ataaaagaac gctcaactga
      601 agatgaaaga aaaattttaa ttcatatgga tgacgcgcag caagaccatg ctgaacaatt
      661 attagctcaa gtgaatcaat tattagcaga taaagatcat ttacatcttg tttttgaata
      721 atatctctat tacgcaagtg tgctgtattc taaagtgcac ttgtgttttc tattttttaa
      781 taaaacctca gcacataatg aacaactttc tattttctat atcacttaaa accatttccg
      841 aaattaaacc tcagcacatt caaagcccca ctttattctt aaaaatattt tttaactcat
      901 atgtattaaa ccgctttcat tataaaaaat atctctatat tttatctgtt tttattaatc
      961 gaaatagcgt gattttgcgg ttttaagcct tttacttcct gaataaatct
//
LOCUS       DAXWFK010000336         1008 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.342, whole genome shotgun sequence.
ACCESSION   DAXWFK010000336 DAXWFK010000000
VERSION     DAXWFK010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.342"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..359
                     /locus_tag="ACGEXS_002775"
     CDS             <1..359
                     /locus_tag="ACGEXS_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HFF8013719.1"
                     /translation="EYFYITYLSRNLKEYRKYYEPLIHKNDKEFKEGMQRARKELNYT
                     ANTNTVATLFSTNDERNRKEKINNVIDLSEKIERTKDMPIKNTITTQLGNKLIGTKKA
                     RFDDKKVVSFGAFEDE"
     gene            352..477
                     /locus_tag="ACGEXS_002776"
                     /pseudo
     CDS             352..477
                     /locus_tag="ACGEXS_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            573..>1008
                     /locus_tag="ACGEXS_002777"
     CDS             573..>1008
                     /locus_tag="ACGEXS_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HFF8013720.1"
                     /translation="MLKKAKFILMATILLSGCSTTNNESNKETKSVPEEMDASKYVGQ
                     GFQPPAEKDAIEFAKKHKDKIAKRGEQFFMDNFGLKVKATNVIGSGDGVEVFVHCDDH
                     DIVFNASIPFDKSIIDSDSSLRSKDKGDDMSTLVGAVLSGFEY"
BASE COUNT          430 a          115 c          188 g          275 t
ORIGIN      
        1 atgaatattt ttatattaca tatttatcaa gaaatttaaa agaatataga aaatattacg
       61 aaccattgat acataaaaat gataaagagt ttaaagaagg tatgcaacga gctagaaaag
      121 agctaaacta tactgctaat acaaatactg tagcaacgtt gtttagtacg aatgatgaaa
      181 ggaatagaaa agaaaagata aataatgtaa tagatttatc cgagaaaatt gaaagaacaa
      241 aagatatgcc aatcaagaat actataacta ctcaattagg aaataaactt attggcacaa
      301 aaaaagctcg ttttgatgat aagaaagtag tgtcgtttgg agcatttgaa gatgaataaa
      361 ataaatgata gagatttaac agaattgagt agctataggg tttatcaaga catcaataaa
      421 gataatgact ttacagttaa cgaaaaacga tttaagcagg cagatgtatt tgaagattta
      481 tatagagaga aactaaaaga cacaaataaa ttaagagagt ataattattt acaaaatgaa
      541 acttttaaaa gcgcataaat aggtgatgag atatgcttaa aaaagcaaaa tttatcttaa
      601 tggcaacgat actactatca ggatgttcaa ctaccaataa cgaatccaac aaagaaacaa
      661 aatctgtacc agaagaaatg gatgcttcaa aatatgtagg acaaggattc caaccacctg
      721 cagaaaaaga tgcgattgaa tttgcaaaga agcataaaga taaaattgct aagcgaggcg
      781 aacaattttt tatggataac ttcggtctaa aagttaaagc tacaaatgtt ataggtagtg
      841 gcgatggtgt agaagtattc gtgcattgtg atgaccacga catcgtattt aatgcgagta
      901 ttccatttga taaatcaatt attgacagtg atagctcatt aagaagtaag gataaaggtg
      961 atgatatgag tactttagtt ggtgcagtac tcagtgggtt tgaatatc
//
LOCUS       DAXWFK010000337         1002 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.343, whole genome shotgun sequence.
ACCESSION   DAXWFK010000337 DAXWFK010000000
VERSION     DAXWFK010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.343"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            3..899
                     /locus_tag="ACGEXS_002778"
     CDS             3..899
                     /locus_tag="ACGEXS_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8013721.1"
                     /translation="MQISNINKSFNKRCVLKNISFDIEQGKCIALIGKNGAGKSTLID
                     ILIGNVNANSGEIFDKDKLLQSENRSIMFQKTMFPDQLKVIEIINLYQSFYENPLPLE
                     EIIELTKFDSSQLNQFVNKLSGGQQRLLDFVLSLIGQPQLILLDEPTSTMDIEIREYF
                     WSIIENLKEDNRTILYTSHYIEEVERMSDKIILIENGEIILNDSTSHIRTNQQSQITL
                     SDEYIRKLKLDKDDLVIQKNHNGTIKIITSNVNDTILYLQQLHINLDDIEIQKVSIVD
                     SYFNNKKQRGSNYDTKLLENRI"
     gene            868..>1002
                     /locus_tag="ACGEXS_002779"
     CDS             868..>1002
                     /locus_tag="ACGEXS_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8013722.1"
                     /translation="MILSYLKIEFKVIMRKKTTLILSILFPVIFYILFTSILELPEDV
                     K"
BASE COUNT          402 a          127 c          139 g          334 t
ORIGIN      
        1 tgattcaaat atctaatatc aacaagtcat ttaataaaag atgtgttcta aaaaatattt
       61 cgttcgatat tgaacaaggt aaatgtatcg ctttaattgg aaaaaatggt gctggaaagt
      121 caacgttaat tgatatatta attggtaatg ttaatgctaa ttctggtgag atatttgata
      181 aagacaagtt attacaaagt gaaaatcgca gtataatgtt ccaaaaaacg atgtttccag
      241 atcaattaaa agttattgag attatcaact tatatcaatc attttacgaa aatccattac
      301 cattggaaga aataatagaa ctgacgaaat ttgattctag tcaactgaac caatttgtaa
      361 ataaactttc tggtggtcaa caacgattac tcgattttgt attatcttta atcggacaac
      421 cacaattgat cttattagat gaaccaacat cgactatgga tatagaaatt agagaatatt
      481 tttggtcaat tattgaaaat ttaaaagaag ataatcgaac gatactctat acatcgcact
      541 atattgaaga agtcgaacgt atgtcagaca aaattattct cattgaaaat ggagaaataa
      601 tacttaatga ttcaacgtca catattagaa ccaatcagca atctcagatt acgttatccg
      661 atgaatatat aagaaagtta aaactagata aagatgattt agttattcaa aaaaatcata
      721 atggcactat caaaattatt acttcaaatg taaatgatac gattttatat cttcaacaac
      781 ttcatattaa tttggatgat attgaaatac aaaaagtctc aattgttgat tcatacttca
      841 acaataaaaa gcaaagggga tctaattatg atactaagtt acttgaaaat cgaatttaaa
      901 gttataatgc gtaaaaaaac aacattaata ttatctattt tatttcctgt tatattctat
      961 atattattta cttcgatatt ggaattgccg gaagatgtta aa
//
LOCUS       DAXWFK010000338         1001 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.344, whole genome shotgun sequence.
ACCESSION   DAXWFK010000338 DAXWFK010000000
VERSION     DAXWFK010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.344"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            389..790
                     /locus_tag="ACGEXS_002780"
     CDS             389..790
                     /locus_tag="ACGEXS_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl peptide receptor-like 1 inhibitory
                     protein"
                     /protein_id="HFF8013723.1"
                     /translation="MKKNITKTIIASTVIAAGLLTQTNDAKAFFSYEWKGLEIAKNLA
                     DQAKKDDERIDKLMKESDKNLTPYKAETVNDLYLIVKKLSQGDVKKAVVRIKDGGPRD
                     YYTFDLTRPLEENRKNIKVVKNGEIDSITWY"
     gene            750..842
                     /locus_tag="ACGEXS_002781"
     CDS             750..842
                     /locus_tag="ACGEXS_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013724.1"
                     /translation="MLKTVKSTRLLGIKKHTELNSCTPNVRKQC"
BASE COUNT          376 a          144 c          142 g          339 t
ORIGIN      
        1 attacttctc tactatgaat catttctttg ttttataaaa aatatatatc aaaacagcta
       61 tatgtataac aatttggagt gtttgtctat ccaacttagc aaacaaaatg tagagaaatg
      121 taaatatcgt tttagcaata aaaattattc aatcatagtt gagtgacgtt tactactatt
      181 attatactat caacaaattc aataatacct caaataaaaa taataccatc aaattcatca
      241 ttattctttt tcgattgcat taaaccttat taatttgcac ttaattttaa ttaatgaagt
      301 gttaactggt aaatgtatga aaccgtttgt aaaatagcag tgtactcgca ttagtaagta
      361 caaatttatt ttacagggag aacttattat gaaaaaaaat atcacaaaaa ctattatcgc
      421 atctacagta atcgctgcag gtcttttaac tcaaactaat gatgctaaag ctttctttag
      481 ttatgaatgg aaaggtttag aaatcgcaaa aaatttagca gatcaagcta aaaaggacga
      541 tgaacgtatt gataagttaa tgaaagagtc tgataaaaat ctaactcctt acaaagctga
      601 aactgttaat gatctgtacc ttattgttaa aaaattaagc caaggtgatg tgaagaaagc
      661 agttgtcaga attaaagatg gtggtcctag agattactat acttttgact taactcgtcc
      721 tttagaagag aacagaaaaa atattaaagt tgttaaaaac ggtgaaatcg actcgattac
      781 ttggtattaa aaaacatact gaattaaata gttgtacgcc aaacgttaga aaacaatgct
      841 aacgtattgg cgtgcttttt tattttaagt aacttccaat ttatttagca tctttacgac
      901 tgtttaataa agtacgtatg attaacacgg ttgccactat atccgttaca atttttatga
      961 ttgttaatac aatcgatttc cttttcacaa tagccacctc g
//
LOCUS       DAXWFK010000339          995 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.345, whole genome shotgun sequence.
ACCESSION   DAXWFK010000339 DAXWFK010000000
VERSION     DAXWFK010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.345"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            89..295
                     /locus_tag="ACGEXS_002782"
     CDS             89..295
                     /locus_tag="ACGEXS_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013725.1"
                     /translation="MNEIETIISEIEKLLTNNTPYSISKNSGVPRQTVTDLKVGKTKI
                     KEAKFKTIIKLYEYQRTLENKTEC"
BASE COUNT          393 a           93 c          151 g          358 t
ORIGIN      
        1 ggcaactaga aaaaaacaat gtattgctaa acgtagttac tgcattagga ttattataat
       61 tataaataga aagagagtgt gattaataat gaatgagatt gaaactatta taagtgaaat
      121 agaaaagtta ttaactaaca atacaccata tagtatttca aaaaactcag gtgtaccacg
      181 tcaaacagtt actgatttaa aggtaggtaa aactaaaata aaagaagcta aatttaaaac
      241 gataatcaag ttatatgaat atcaaagaac attagaaaat aaaacagaat gttaacaaaa
      301 atattttgtt aggttaggtg gacaagaaat taaatttcta atgatttcat ggttgtctgt
      361 ccacgttttg tccacctcaa taagataatc tcttagtttt agaaattgta catggttaaa
      421 taatcaaaaa tgcatattat gaaattatca ttgccattaa atcttgaatg atctttaagc
      481 gatgatataa aaggctgaac acagtaaata agttaacttt aatgcagtat tctatactat
      541 acatattttg taataatgtt tatgcacata atttttcatt gacaattttt tatattaaat
      601 tgtgtaattg gcatatatga tgatttgtga taattatatt gtaatatagc atttggtttt
      661 atgattctaa aattaataga tttgaaaagt atactttatt tcatgaaagt ttttggtaca
      721 tttatttgaa aatgttttta ttacaaagtt acatggctaa atgtttctta acaaaattaa
      781 tttttcgagt gtaaagaatc gtgtggtaaa cataatttat atagattata aggaggtgaa
      841 aatggtcgag gaagttaaaa cgataaaata gtttgaatgt tttgatttca attctggtag
      901 gactattgaa tttgcaatct aataagaatg tgaggtttga tttaatatga agtcatattc
      961 tttgaataaa atattacttt ctcaccatta gtaaa
//
LOCUS       DAXWFK010000340          972 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.346, whole genome shotgun sequence.
ACCESSION   DAXWFK010000340 DAXWFK010000000
VERSION     DAXWFK010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.346"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..>972
                     /locus_tag="ACGEXS_002783"
     CDS             <1..>972
                     /locus_tag="ACGEXS_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8013726.1"
                     /translation="TKSLVKLFKTFPTKIIGLLKLFENNNDPYIVERLYASVLGATLR
                     IDICEIHIEIANYIYEEIFDKEMVYPHILMRDYARQTIEYISLSKDISNINLEKIRPP
                     YKSNWYKKEYSNLNIDDYIKSLKNKLDSHLHFSIDKIKNSMTTEYGRGTGAYGDFGRY
                     VFGYAVRNWVKGFKSDQDLSNIALMRIFEMGYDAKLHGEFDMWVNRYDNFNNSIERIS
                     KKYQWIAYYEILAKLVDKFPDVQYSGLWDDYIRDIDPTLLLLEIDKESKILVPSPLPS
                     HQSNEWVKNTKVFDETKLFLEIDIDNHRYICLSSKFNFEKREKEIPFE"
BASE COUNT          392 a          102 c          152 g          326 t
ORIGIN      
        1 actaaatctt tagtaaaatt attcaaaaca tttccaacta aaattattgg tttattaaaa
       61 ttatttgaaa ataacaatga tccatatata gtcgaaagat tatatgcttc tgttttaggg
      121 gcgacacttc gaatcgacat ttgtgaaata catatagaga ttgctaatta tatttatgaa
      181 gaaatatttg ataaagaaat ggtatatcct catattttaa tgagagatta tgctaggcaa
      241 acaattgaat atattagttt atcaaaagat atatctaata taaatttaga aaaaattagg
      301 ccaccttata aaagtaattg gtataaaaaa gaatattcga atttaaatat agacgattac
      361 ataaaatctt taaaaaacaa attagatagc catttacatt tttctatcga taaaattaaa
      421 aactcaatga caacggaata tggaagagga actggggcat atggcgattt tggcagatat
      481 gtatttggtt atgctgtacg aaactgggta aagggtttta aaagtgatca agacttaagt
      541 aatatagcat taatgaggat ttttgaaatg ggttatgatg caaaattaca tggtgaattt
      601 gatatgtggg ttaatcgtta tgataacttt aataattcaa ttgaaagaat tagcaaaaaa
      661 tatcaatgga ttgcttacta tgaaatttta gctaaacttg tagataaatt tcctgatgtt
      721 caatattctg gactatggga tgattatatt agagatattg atcctacttt actattactt
      781 gaaattgata aagaatcaaa aatactagta ccctcaccac ttccttcgca tcaatcgaat
      841 gaatgggtaa aaaataccaa agtttttgat gaaacaaaat tatttttaga aatagatata
      901 gacaatcata gatatatatg tttaagctca aagtttaatt ttgaaaagag agagaaagaa
      961 ataccatttg aa
//
LOCUS       DAXWFK010000341          969 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.347, whole genome shotgun sequence.
ACCESSION   DAXWFK010000341 DAXWFK010000000
VERSION     DAXWFK010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..969
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.347"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(164..856)
                     /locus_tag="ACGEXS_002784"
     CDS             complement(164..856)
                     /locus_tag="ACGEXS_002784"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFF8013727.1"
                     /translation="MNYSPKQGVRSHGLPHDPFKSSTVPRPIGWISTVSKDGKDNLAP
                     YSQYQNLTWDPPMVMFAANQSVLGDHERKDTVKNAEETGWFVWNMATYDLREAVNLSS
                     KALPPEEDEFNFAGVTKEKCIEAPGYRVKESPVHFECEYVQTIRIPTGDPVSTVDIVI
                     GRVAQVHIDDKVILDNGKLDIKSIKPIARLGYYDYTVVNEIFEMKAPAASKEELAGLE
                     GRNFDNQSDEKI"
BASE COUNT          298 a          194 c          136 g          341 t
ORIGIN      
        1 aacctagtgt aatatatgtg taattctaaa agattcttct ttaaaaatat aaataccacg
       61 acatattgct ttaacatttt catttataaa gcgaaaaaat gcatcgctac taagttgaat
      121 gtttagtaag gatgcattga attcactaaa atgattaaat tacttatatc ttttcatctg
      181 attgattatc gaaatttctt ccttctaaac ctgctaactc ttctttagaa gctgcaggtg
      241 ctttcatttc aaatatctca ttcactactg tgtaatcgta atatcctaat ctggcaatag
      301 gtttaatcga cttaatgtcc aatttaccat tatcaagaat aaccttatcg tcaatatgaa
      361 cttgggcaac tcttcctata acaatatcta cggtagatac tggatctcca gttggaatac
      421 gaatcgtttg aacgtactca cattcaaaat gaactggcga ttcttttaca cgatatcctg
      481 gagcttctat acatttttcc tttgttacac ctgcaaaatt aaattcatcc tcttctggtg
      541 gcaatgcttt cgatgataaa ttaactgctt ctcttaaatc atacgttgcc atattccaca
      601 caaaccaacc tgtctcttca gcatttttca ctgtatcttt acgttcgtga tcaccaagaa
      661 cggattgatt tgctgcgaac ataaccatag gcggatccca agttaagttt tgatactgac
      721 tataaggcgc taaattatct ttcccatctt tcgatacagt agagatccac cctattggac
      781 gtggtactgt actactttta aatgggtcgt gcggtaaacc atgacttctt acaccttgtt
      841 ttggcgaata attcatacta tcttcacccc ttataagtaa ttacatttaa ggttacgccc
      901 tctttacata agcgtctaat ataaataaac aatttattta taagtagaaa ctatatatga
      961 cgtggttgc
//
LOCUS       DAXWFK010000342          930 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.349, whole genome shotgun sequence.
ACCESSION   DAXWFK010000342 DAXWFK010000000
VERSION     DAXWFK010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..930
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.349"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            271..771
                     /locus_tag="ACGEXS_002785"
     CDS             271..771
                     /locus_tag="ACGEXS_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HFF8013728.1"
                     /translation="MTFEEKLSEIYNKIANEISGMIPVEWDQVFTIAYVNDRGGEIVF
                     NYTKPGSDELNYYTYIPREYNVSEKVFYDLWTDLYRLFKKLRNAFKEEDLEPWTSCEF
                     DFTRDGKLNVVFDYVDWMNSEFGPIAKENYYMYKKFGVLPETEYEINKVKEIDQYVKE
                     QDEAEL"
     gene            783..>930
                     /locus_tag="ACGEXS_002786"
     CDS             783..>930
                     /locus_tag="ACGEXS_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein YezG family protein"
                     /protein_id="HFF8013729.1"
                     /translation="MTFEEKLSQMYNKIASEISGMIPVEWEQVFTIAYVTDQAGEVIF
                     NYTKP"
BASE COUNT          363 a           90 c          189 g          288 t
ORIGIN      
        1 aatagttaaa taaatagaaa gacaaaataa tgagtaggag ttttagggct attatttaca
       61 aataatataa tactgcaaag actcgaaatt atttagaatt ccagttatcg taacaatagt
      121 tgcagttatt ttagttccat tagtgtattt aggtttaaaa tgagtaatat taatttgaag
      181 aagcattaag cgacgtagag gtgttgttta gtgcttttta tttaacggaa atgaaaccaa
      241 tatctatttt gaaaatatag gggcgataac atgactttcg aagaaaaact aagtgaaata
      301 tacaacaaga ttgcgaatga gattagtggg atgataccag tagaatggga ccaagtattt
      361 acgatagctt atgtaaatga tagaggtgga gagatcgttt ttaattatac taaaccaggt
      421 agcgatgaat tgaattatta cacatatatc cctagagagt ataatgtctc tgaaaaagta
      481 ttttatgatt tgtggacgga tttatataga ttgtttaaga agttaagaaa cgcatttaaa
      541 gaagaagatt tagaaccatg gacatcatgt gaatttgact ttacaagaga tggtaaattg
      601 aatgttgtat ttgattatgt tgattggatg aattcagaat ttggtccaat agcaaaagaa
      661 aattattata tgtacaaaaa gtttggtgtt ttaccagaaa cggaatatga aattaataaa
      721 gttaaagaaa tcgatcaata tgttaaagag caagatgaag ctgaactata gggggagata
      781 acatgacttt cgaagagaaa ctaagtcaaa tgtacaataa aattgcaagt gagatcagtg
      841 gaatgatacc agtagaatgg gagcaagtat ttacaatagc ttatgtaact gatcaagctg
      901 gggaagtcat ttttaattat actaaaccag
//
LOCUS       DAXWFK010000343          923 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.350, whole genome shotgun sequence.
ACCESSION   DAXWFK010000343 DAXWFK010000000
VERSION     DAXWFK010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.350"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            437..>923
                     /locus_tag="ACGEXS_002787"
     CDS             437..>923
                     /locus_tag="ACGEXS_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFF8013730.1"
                     /translation="MLLCDVRVIYKNPKYKVIQHNGEYLLVDLVSTWFVYFFPFINWF
                     IPKKYAIISEEEFENLNVVKPNKNNVFWSVIGSSVLFGVTLRKYIHVFDVQLDKLVVM
                     ILCALALICVIVFYFNLNRKLKLKVFDTNIEKNKRVILIPTFKLGCFLVFGYIFAGSF
                     SI"
BASE COUNT          326 a           94 c          170 g          333 t
ORIGIN      
        1 tggtagtatc gcatatttaa acggtggttc aaaaaaatat aatcatatct taaatcagga
       61 aaatagatag taatgtttga atataccaag tgaaaatgcc cattgtgatt gataaaggaa
      121 aaaactgttt taatttaaaa atgaaatata gagcgtgtta ttttgtatga tagctcttat
      181 aacattggcg tttttaatcc aatagtatgg acggttttag ctggttttgt tgcggaatct
      241 gtttagacaa ttcttaaaaa ttggactttt catactaata aatttggatt aagagataaa
      301 actaattgga taagacatta aatagacgct gtttaaaaat accacagaaa aagctgtaga
      361 tagtgttgga acgatagttg gtgaaggtac aaaaagtatt ggggatcatt taaaactaat
      421 gaaacgggag tggtaaatgt tgctttgcga tgtcagagtc atttataaaa atccgaaata
      481 caaagtcatt caacataacg gtgaatactt attagtcgat ttagtaagca cttggttcgt
      541 gtactttttt cctttcatta attggttcat tccaaaaaag tacgcgataa ttagcgaaga
      601 agaatttgaa aatttaaatg ttgttaaacc aaataaaaat aatgttttct ggtcagttat
      661 aggaagttcg gttttgtttg gagttacttt aaggaaatac atacatgttt ttgatgttca
      721 attagataag ctagttgtaa tgatattgtg tgctctcgct ttaatttgtg ttatagtttt
      781 ttattttaac ttaaatagaa agcttaagtt aaaagtgttt gatacaaata ttgaaaaaaa
      841 taagagagtt atattaatac caacgtttaa acttggctgt tttttagttt tcggatatat
      901 tttcgctgga agtttttcaa tat
//
LOCUS       DAXWFK010000344          914 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.351, whole genome shotgun sequence.
ACCESSION   DAXWFK010000344 DAXWFK010000000
VERSION     DAXWFK010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.351"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..681)
                     /locus_tag="ACGEXS_002788"
     CDS             complement(<1..681)
                     /locus_tag="ACGEXS_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HFF8013731.1"
                     /translation="MNTIYEPSSICMIRTPLLSVEFFNLFLNTEQIKYSDLQLNAQMK
                     ESILTTTFNLYCTLQEINFDGDNKKVRDAKESLLKYLIRMSTRPTPFGLLSGINLGHF
                     VNEPTRLKVGNSIQKYVKVDGEWLYKLVSYIESIDEYYQNLKVIWNSKAHIINDRIYL
                     NEQSAIYLNNNKDTSFSIKNSELLVFIKTTVTNNNITFSNLAEKINQEFEINDITKVK
                     VYIHNLVSK"
     gene            complement(746..889)
                     /locus_tag="ACGEXS_002789"
     CDS             complement(746..889)
                     /locus_tag="ACGEXS_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gallidermin/nisin family lantibiotic"
                     /protein_id="HFF8013732.1"
                     /translation="MEKVLDLDVQVKANNNSNDSAGDERITSHSLCTPGCAKTGSFNS
                     FCC"
BASE COUNT          305 a          144 c          114 g          351 t
ORIGIN      
        1 ctttgagaca agattatgga tatatacttt cactttagta atatcattaa tttcaaattc
       61 ttgatttatc ttttcagcta aatttgaaaa agtaatatta ttattcgtca cagtggtttt
      121 gatgaatacg agtaattcgc tatttttaat ggaaaaagat gtatctttat tgttgtttaa
      181 ataaatagca ctttgttcat ttaaatatat tcgatcattg ataatatgtg ctttactatt
      241 ccaaataact ttcaaatttt gataatattc atcaatactc tcaatataac taaccagttt
      301 atacaaccat tcgccatcga ctttcacata tttttgaata ctatttccaa ctttaagacg
      361 tgttggttca tttacaaaat gccccaaatt aataccactt aataatccaa atggagttgg
      421 acgtgttgac attctaatta aatattttaa taaactttct ttagcatctc taaccttctt
      481 gttatcgcca tcaaaattta tttcttgtaa agtacaatat aagttaaatg ttgttgttag
      541 gattgattct ttcatctgag catttaattg taagtcgcta tatttaattt gttcagtatt
      601 taaaaataaa ttgaaaaatt caactgataa taaaggtgtt ctaatcatac aaatacttga
      661 cggttcgtaa atagtgttca tttgtaagca cctcacatat ttgaatagac aaatagagcc
      721 ctttcaggct ctatttgctg taatcttaac agcagaagct attaaaacta ccagtcttag
      781 cacaaccagg agtacataaa ctatgacttg taatacgttc gtcacctgct gaatcatttg
      841 agttattgtt tgctttaact tgcacgtcta aatcaagaac tttttccata gataaaacct
      901 cctattatta gttg
//
LOCUS       DAXWFK010000345          855 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.352, whole genome shotgun sequence.
ACCESSION   DAXWFK010000345 DAXWFK010000000
VERSION     DAXWFK010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..855
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.352"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..679
                     /locus_tag="ACGEXS_002790"
     CDS             <1..679
                     /locus_tag="ACGEXS_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="superantigen-like protein SSL6"
                     /protein_id="HFF8013733.1"
                     /translation="AKATLVLGLLATGVITTESQTVKAAESTQGQHNYKSLKYYYSKP
                     SIELKNLDGLYRQKVTDKGVYVWKDRKDYFVGLLGKDIEKYPQGEHDKQDAFLVIEEE
                     TVNGRQYSIGGLSKTNSKEFSKEVDVKVTRKIDESSEKSKDSKFKITKEEISLKELDF
                     KLRKKLMEEEKLYGAVNNRKGKIVVKMEDDKFYTFELTKKLQPHRMGDTIDGTKIKEI
                     NVELEYK"
BASE COUNT          346 a           90 c          177 g          242 t
ORIGIN      
        1 agctaaagca acattagtat tgggattgtt agctactggt gtaataacaa cagaaagtca
       61 aacagtaaaa gcggcagaat caactcaagg tcaacacaat tataaatcgt taaaatacta
      121 ctatagcaag ccaagtatag agttaaaaaa tcttgatggt ttgtatagac agaaagtgac
      181 agataaagga gtatatgttt ggaaggatcg aaaagattat tttgttggct tgcttggtaa
      241 agatattgaa aaataccctc aaggtgagca tgataagcaa gatgcatttt tagtcatcga
      301 ggaggaaact gttaatggaa gacaatattc aattggtggt ttaagtaaga caaatagtaa
      361 agaatttagt aaagaagtcg atgttaaagt aacaagaaaa attgatgaat catcggaaaa
      421 gtctaaagat agtaaattta aaattactaa agaagaaatc tcgttaaaag agttggactt
      481 taaattaaga aaaaaattga tggaagaaga aaaattatat ggtgctgtta ataatagaaa
      541 aggtaaaatt gtagttaaaa tggaagatga taagttttat actttcgaac ttacaaaaaa
      601 actacaaccg catcgcatgg gtgacacgat agatggtacc aaaatcaaag aaattaatgt
      661 tgagctagaa tataaataat ctttggacaa gcagactagt aattgtaggg aagttaagcg
      721 ataacatatt gcttagcttc ttttttattt tgttatgatg aaaaaaggag cgggtttatg
      781 atcaagtttt tggaaaaacg gttgatactt atagtcgcgc gttgtccttt tcgtgacatg
      841 aaacaatgtg gaaaa
//
LOCUS       DAXWFK010000346          848 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.353, whole genome shotgun sequence.
ACCESSION   DAXWFK010000346 DAXWFK010000000
VERSION     DAXWFK010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..848
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.353"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            38..724
                     /locus_tag="ACGEXS_002791"
     CDS             38..724
                     /locus_tag="ACGEXS_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFF8013734.1"
                     /translation="MTEYLLSAGICMAIVSILLIGMAISNVSKGQYAKRFFYFATSCL
                     VLTLVVVSSLSSSANASQTDNGVNRSGSEDPTVYSATSTKKLHKEPATLIKAIDGDTV
                     KLMYKGQPMTFRLLLVDTPETKHPKKGVEKYGPEASAFTKKMVENAKKIEVEFDKGQR
                     TDKYGRGLAYIYADGKMVNEALVRQGLAKVAYVYKPNNTHEQLLRKSEAQAKKEKLNI
                     WSEDNADSGQ"
BASE COUNT          329 a          109 c          163 g          247 t
ORIGIN      
        1 aatattaaaa ttaactaaaa agaaagaggt gttagttatg acagaatact tattaagtgc
       61 tggcatatgt atggcaatcg tttcaatatt acttataggg atggctatca gtaatgtttc
      121 gaaagggcaa tacgcaaaga ggtttttcta tttcgctact agttgtttag tgttaacttt
      181 agttgtagtt tcaagtctaa gtagctcagc aaatgcatca caaacagata atggcgtaaa
      241 tagaagtggt tctgaagatc caacagtata tagtgcaact tcaactaaaa aattacataa
      301 agaacctgcg acattaatta aagcgattga tggtgatacg gttaaattaa tgtacaaagg
      361 tcaaccaatg acattcagac tattattggt tgatacacct gaaacaaagc atcctaaaaa
      421 aggtgtagag aaatatggtc ctgaagcaag tgcatttacg aaaaaaatgg tagaaaatgc
      481 aaagaaaatt gaagtcgagt ttgacaaagg tcaaagaact gataaatatg gacgtggctt
      541 agcgtatatt tatgctgatg gaaaaatggt aaacgaagct ttagttcgtc aaggcttggc
      601 taaagttgct tatgtttata aacctaacaa tacacatgaa caacttttaa gaaaaagtga
      661 agcacaagca aaaaaagaga aattaaatat ttggagcgaa gacaacgctg attcaggtca
      721 ataatgctca ttgtaaaagt gtcactgctg ctagtggcac ttttataatt tttagatcac
      781 gatatgattt attatcaatt cagaattaaa aaagtaaata gtatcaaaag taagtgtatt
      841 taatatta
//
LOCUS       DAXWFK010000347          844 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.354, whole genome shotgun sequence.
ACCESSION   DAXWFK010000347 DAXWFK010000000
VERSION     DAXWFK010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.354"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            358..788
                     /gene="tnpA"
                     /locus_tag="ACGEXS_002792"
                     /pseudo
     CDS             358..788
                     /gene="tnpA"
                     /locus_tag="ACGEXS_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500412.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          325 a          116 c          172 g          231 t
ORIGIN      
        1 acgaaaatgc gcttgtaata agcttttttc aattctagtc aggggcccca acacagagaa
       61 tttcgaaaag aaattctaca ggcaatgcga gttggggcgg ggccccaaca aagagaaatt
      121 ggattcccaa tttctacaga caatgcaagt tggggtggga cggcgaaata aattttgcga
      181 aaatatcatt tctgtcccac tccccagaaa aggttctacc attgtcaaaa aaatgcatct
      241 ctacatgtta gagtaaatat tggtcagcca accaaaataa tcaacacgag gagatgctat
      301 ttaatgtcat ctgacacaaa cagtttagca catacgaaat ggaattgtaa gtaacatatt
      361 gtctttgcac ctaaatacag aagacaagcg atatatggaa aaataaaaaa agatataggg
      421 attatattac gtcaattata tgaaagaaaa ggtgtagaga taattgaagc agaggtatgt
      481 aaagatcata tccatatgtt agtaagtata ccacccaaac ttggggtatc atcatttgtt
      541 ggctatttaa aaggaaaagt aatttaatga tatttgatag acatgctaac ttaaagtata
      601 gatatggaaa tagaaagttt tggtgtaaag gattttatgt ggatacagta ggtagaaata
      661 aaaaagtgat tgaaaattat attcgtaatc aattacaaga ggatatcgtt gcagatgaaa
      721 tttcaatgga agaatattta gatcctttca ctggagagaa aaataaaaaa agaaagaaaa
      781 aagagtaaac ctttaggatt gctggaatag tagtgcagtt ggctgacttg tcagtgccct
      841 ttta
//
LOCUS       DAXWFK010000348          824 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.355, whole genome shotgun sequence.
ACCESSION   DAXWFK010000348 DAXWFK010000000
VERSION     DAXWFK010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..824
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.355"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..541
                     /locus_tag="ACGEXS_002793"
     CDS             <1..541
                     /locus_tag="ACGEXS_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8013735.1"
                     /translation="SLLTFPLDIINEKQNEWRQRLMVTPFTFTSYYISKVVKTMLQFA
                     IAILVIFMVGHFYKGVAMSAVQWLESGIFLWLGASLLITFGILFSLLNDIQKTSALAN
                     IVTIGLAVLGGLWFPINTFPNWLQHVAHVLPSYHLRKLGVDIASNHHINLISFAIILL
                     YALGSIIAVYCISHFKRAE"
     gene            545..>824
                     /locus_tag="ACGEXS_002794"
     CDS             545..>824
                     /locus_tag="ACGEXS_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFF8013736.1"
                     /translation="MKFLKDTSIAEISSILYLIFPIAGIFFNEVYGPKWLYIISVIVF
                     SLSYLILVIVNNRLNTLMFYILLIIHYFIICYFVFSVHPMLSLFFFYSA"
BASE COUNT          250 a           98 c          130 g          346 t
ORIGIN      
        1 tagtttacta acttttccat tagatattat taatgaaaaa caaaatgaat ggcgccaaag
       61 attaatggta acaccattta cttttactag ttattatatt tcaaaagtag tgaaaactat
      121 gctgcaattt gcaatagcga tattagttat ttttatggtt ggacattttt ataaaggtgt
      181 tgcaatgagt gcagttcaat ggttagagtc aggaatattt ttatggttag gtgcgtctct
      241 attaataact tttggcatat tattttcttt gttaaatgat attcaaaaaa caagtgcttt
      301 agctaatatc gtaacaattg gtttagcagt attaggtgga ttgtggtttc cgataaacac
      361 atttccaaat tggcttcaac atgttgctca tgttttaccg agctatcatt tgcgtaaact
      421 aggtgtagat attgcttcaa atcatcatat caatttaata tcatttgcta taatactctt
      481 gtatgcttta gggagtataa tagcagtata ttgtattagt cattttaaaa gggcggaata
      541 aaatatgaaa tttttaaaag atacttcaat tgctgaaata tcgtctatac tttatctgat
      601 ttttcctatt gccggtatat tttttaatga agtatatggt cccaaatggt tgtatattat
      661 atcagtcatt gtcttttcgt tgtcgtatct tatattagtt atagtaaata atagacttaa
      721 tacattaatg ttttacattt tgttgattat tcattatttt attatttgtt attttgtttt
      781 cagtgtacat ccaatgctaa gtttgttttt cttttatagt gctt
//
LOCUS       DAXWFK010000349          819 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.356, whole genome shotgun sequence.
ACCESSION   DAXWFK010000349 DAXWFK010000000
VERSION     DAXWFK010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.356"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            8..>819
                     /locus_tag="ACGEXS_002795"
     CDS             8..>819
                     /locus_tag="ACGEXS_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HFF8013737.1"
                     /translation="MGSVLATTLILGGCSTMENESKKDTKTETKSVPEEMEASKYVGQ
                     GFQPPAEKNVIEFAKKHRKEFEKVGEQFFKDNFGLKVKATNVVGKDDGVEVYVHCEDH
                     GIVFNASLPLYKDAIHQKGSMRSNDNGDDMSMMVGTVLSGFEYRAQKEKYDNLYKFFK
                     ENEKKYQYTGFTKEAINKTQNVGYKNEYFYITYSSRSLKEYRKYYEPLIRKNDKEFKE
                     GMERARKEVNYAANTDAVATLFSTKKNFTKDNTVDDVIELSDKLYNLKNKPD"
BASE COUNT          347 a          101 c          155 g          216 t
ORIGIN      
        1 aaaattcatt ggatcagttt tagctacgac attaatttta gggggatgtt ccacgatgga
       61 aaatgaatca aaaaaagaca caaaaacaga aacaaaatct gtaccagaag aaatggaagc
      121 ttcaaaatat gtaggccaag gcttccaacc gcctgcagaa aaaaatgtga ttgaatttgc
      181 gaagaagcat cgtaaagaat ttgaaaaagt aggtgaacaa ttctttaaag ataactttgg
      241 actaaaagtt aaagctacaa atgttgtagg taaagatgat ggtgtagaag tttatgtgca
      301 ttgtgaagat catggcattg tatttaatgc aagtctacct ttgtacaaag atgccatcca
      361 tcaaaaagga tcaatgcgca gtaatgacaa tggtgatgat atgagtatga tggtgggtac
      421 agtgctgagt ggctttgaat atcgagcgca aaaagaaaag tatgataact tatataaatt
      481 cttcaaagaa aatgaaaaga aatatcaata tacaggcttt acaaaagagg caattaacaa
      541 gacacaaaat gtcggatata aaaatgaata tttttatatt acatactctt ctagaagttt
      601 aaaagaatat cgaaagtatt atgaaccact gattcgaaaa aatgataaag aatttaaaga
      661 aggaatggaa cgagcaagaa aagaagtgaa ttacgctgca aatacagatg ctgttgctac
      721 acttttttct actaagaaaa actttactaa agacaataca gtagatgatg taatcgaact
      781 aagtgataaa ttatataatt taaaaaataa accagataa
//
LOCUS       DAXWFK010000350          817 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.357, whole genome shotgun sequence.
ACCESSION   DAXWFK010000350 DAXWFK010000000
VERSION     DAXWFK010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..817
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.357"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            517..725
                     /locus_tag="ACGEXS_002796"
                     /pseudo
     CDS             517..725
                     /locus_tag="ACGEXS_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          328 a          115 c          111 g          263 t
ORIGIN      
        1 atattagtac aatagattat agcgaattag atgaaaagat aggtttattg atttaatata
       61 tatagacgtg ataatatcaa tgtttatatt aaatgaaacg aatttgaaaa tttcgaaaca
      121 gcttaagata gcaaattgaa tagccttatt gataatgcag aatcgtctac acttagttga
      181 acaaattcta tgagaataga tattgttaaa ctatttgggt aggtgatttt attttgacaa
      241 gagtcagaag attatttagt tcatagttta agttacaaat ggctaaactt tatgaaaatg
      301 gtatgactag aatcgaaatt atactcgaat acgatttaac actctcaatc ttctcgaact
      361 gaataaatac tgggtcattc aatcatcaag acaacttaat aagtgatgat aaatgttcat
      421 accattttgc aaatataacc agatactctc aacaaaatgt agagttcaaa attaaaaatg
      481 tccctaattg gcacattgct gtatgataat aaattcaact tagatttcaa caatataact
      541 aatgatgctt atatttaccg cgatatcaaa ccaaacagcc catccacaaa caaattagaa
      601 tatatcatgt taataatagt aaatgatata tcttccaaag accgcaaact gtagcagaac
      661 atgagaacct tgcgattttg aaatggtcta ataaatcaaa tatcattgaa actttaatca
      721 cataaattaa cagtagtcac ctgttcgcag ttctataaat tacaaatgca ataaaggcta
      781 aagtaaacta attactttag cctttattaa taattta
//
LOCUS       DAXWFK010000351          816 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.358, whole genome shotgun sequence.
ACCESSION   DAXWFK010000351 DAXWFK010000000
VERSION     DAXWFK010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.358"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            40..>816
                     /locus_tag="ACGEXS_002797"
     CDS             40..>816
                     /locus_tag="ACGEXS_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HFF8013738.1"
                     /translation="MRYLKKLAWFISVIILGIFIIGCDSSSDTGEKAKEDSKEEQIKK
                     SFAKTLDMYPIKNLEDLYDKEGYRDSEFKKGDKGMWTIYTDFAKSNKPGELDDEGMVL
                     NLDRNTRTAKGHYFVTTFYRNGKLPDEKNYKIEMKNNKIILLDEVKDDKLKQKIENFK
                     FFGQYANLKELRKYNNGDVSINENVPSYDVEYKMSNKDEIVKELRSRYNISTEKSPIL
                     KMHIDGDLKGSSVGYRKLEIDFSKRENSKLSVIEFLSYKPA"
BASE COUNT          341 a           80 c          161 g          234 t
ORIGIN      
        1 aaaaaatagt attgataagg aatattaggg tgtgaaaaaa tgcgatatct aaaaaagctt
       61 gcatggttca taagtgttat tattttgggc atttttataa taggttgtga tagttcaagc
      121 gatactgggg aaaaagcaaa agaagattca aaggaagaac aaatcaaaaa gagctttgcg
      181 aaaacattag atatgtatcc aattaagaat ctcgaggact tatatgacaa agaaggatat
      241 cgagatagcg aatttaaaaa aggtgacaaa gggatgtgga cgatatatac agatttcgcc
      301 aaaagtaata aaccgggtga attggatgat gaaggtatgg ttttaaatct ggataggaat
      361 actagaaccg ctaaaggcca ttattttgtt actacatttt accggaatgg taaactacca
      421 gatgaaaaga attataaaat tgaaatgaaa aataataaga ttattttatt agatgaagta
      481 aaagatgata agctcaagca gaaaatagaa aactttaaat ttttcggtca atatgcaaat
      541 cttaaagaat tgagaaaata taataatggt gatgtttcaa ttaatgaaaa tgttcctagt
      601 tatgatgttg aatacaaaat gagcaataaa gatgaaatag ttaaggaatt aagaagtcgt
      661 tataatattt cgactgaaaa atcacctata ttaaaaatgc atattgatgg ggacctgaaa
      721 ggcagttctg taggttatag aaagttagaa attgactttt caaaacgtga aaacagcaaa
      781 ttatcagtca ttgaattttt aagttataaa ccagcg
//
LOCUS       DAXWFK010000352          781 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.360, whole genome shotgun sequence.
ACCESSION   DAXWFK010000352 DAXWFK010000000
VERSION     DAXWFK010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.360"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(356..>781)
                     /gene="sdpA"
                     /locus_tag="ACGEXS_002798"
     CDS             complement(356..>781)
                     /gene="sdpA"
                     /locus_tag="ACGEXS_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic endopeptidase
                     SdpA"
                     /protein_id="HFF8013739.1"
                     /translation="LTLSQVIYIIGGALLIYGLDNLYLYFHDVPANEQQLEQAIRNTP
                     FYISIFTVTIIPAIVEEIVFRGMIIRVIFRKHLFLGLIVSSLVFASLHESDTWIGYLP
                     YLYSGLIFGLIYIKTKRLEVVIFMHFLNNLLALLFIIWR"
BASE COUNT          311 a          102 c          117 g          251 t
ORIGIN      
        1 tcagctaagt aaaagctcga gttataaaaa agtctaaatt gattcaacag gagtagaaaa
       61 ggaaattagt cttgcatatt ttagaaaatg aataaaaaca gttactatat gcaactcaag
      121 aattaaatat tatttaagcc acatacctat tagagtacaa aatatacaag ttgtacttat
      181 atctaggtat gtgacttttg attttaagcg taaagtaaaa gtttttatgt ttgtattagt
      241 tctaaattat tttgcacttg atgacacgta tttttgagtt taagtggtga ctcaagttca
      301 ataaaataaa tatcagatag ataatttgaa aataatatga aaggttatct caaaattatc
      361 tccatattat aaagagcaga gctaacaagt tatttaagaa gtgcatgaat attacaactt
      421 ctaatcgttt tgtttttata taaattaggc caaaaatcaa accagaatat aagtaaggta
      481 aataaccaat ccaagtgtca gattcgtgta atgatgcaaa aactaaacta gacacaatta
      541 accctaaaaa caagtgtttt ctgaagataa cccttattat cataccgcga aaaacaattt
      601 cttccacaat agcggggatg atggtaacag tgaaaataga aatatagaat ggtgtatttc
      661 gtattgcttg ctctaattgt tgttcatttg ccggtacgtc atgaaaatat aaataaagat
      721 tatctaaccc ataaattaat agtgcaccgc ctataatata aataacttga gacaatgtta
      781 a
//
LOCUS       DAXWFK010000353          780 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.361, whole genome shotgun sequence.
ACCESSION   DAXWFK010000353 DAXWFK010000000
VERSION     DAXWFK010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.361"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(11..484)
                     /locus_tag="ACGEXS_002799"
     CDS             complement(11..484)
                     /locus_tag="ACGEXS_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HFF8013740.1"
                     /translation="MSKIRSFTILSLLIYLAMMCYTVVTYPKLPTKVPIHYNLAGDAD
                     NFADKWVLLLINSAFIVIWLIFFIAGRYYERFAKWSHYNHTPREIRAIKLFLSTLNLE
                     IMSYMSIFTVLEIWQIQHHHQFNLLWFNMIFIIIIGLTLVIFCLLPTIHKMRDSQ"
     gene            complement(674..>780)
                     /locus_tag="ACGEXS_002800"
     CDS             complement(674..>780)
                     /locus_tag="ACGEXS_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013741.1"
                     /translation="YGSKEKSIRLSFAFESNENIKHALYKIYTYVKKV"
BASE COUNT          272 a          124 c          105 g          279 t
ORIGIN      
        1 gtttatattt ttattgagaa tctctcattt tatgaattgt aggaagtaaa caaaatatga
       61 caagcgtcaa accaatgata atgataaata tcatattaaa ccatagtaaa ttgaattgat
      121 gatggtgttg tatttgccaa atttctaata ctgtgaagat agacatatag ctcataatct
      181 ctaaatttaa cgtacttaaa aataatttaa tcgctcgaat ttcacgtggt gtatgattat
      241 aatgtgacca tttggcaaat cgttcatagt atctacctgc aatgaaaaat ataagccaaa
      301 tcactataaa tgcgctatta atcaaaagca gcacccattt atcagcaaaa ttatcagcat
      361 cccctgctaa attataatga ataggcactt tggttggtaa ttttggatag gtcactactg
      421 tatagcacat catagctaag taaataagta gacttaatat tgtaaaagac ctgattttag
      481 acattctatc gcctctttct ttacatttta tgtataacac tctgcctatt ttacctttta
      541 attacattac cccaaacgat taaacaatat gtaatgatac tataattgcg tcaggagtat
      601 ccgcttgtta aatgtgcata gcttatattt agctgtttaa catgccacat aatgattcga
      661 attattgttt taattaaacc tttttcacat atgtataaat tttatagagc gcatgcttaa
      721 tattttcatt tgattcaaag gcaaaagata gccttatact cttttcttta ctgccataaa
//
LOCUS       DAXWFK010000354          778 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.362, whole genome shotgun sequence.
ACCESSION   DAXWFK010000354 DAXWFK010000000
VERSION     DAXWFK010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.362"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            75..728
                     /locus_tag="ACGEXS_002801"
     CDS             75..728
                     /locus_tag="ACGEXS_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HFF8013742.1"
                     /translation="MELKVDDFVKNIKRPYLTVLGVFVVAVSLFFDAIMFFYAKLYDK
                     LPMYLLVFMAFTAVILIMMYIQEKNENYKVEKRYVVRYLTLNVIVGYTLPLLFVSIYV
                     FGVVGFGFDVFNYCLGIILMLFISWLGLFLFYKNEFDSENPNPAVNAIAIIIKLFAFG
                     GIFYISLIVPVTQQEEIFIGLSIFINIIVDALLVRSYFNYALYKSIKKDIENEGKTM"
BASE COUNT          257 a           72 c          139 g          310 t
ORIGIN      
        1 atgcattttt ttggcgaaga agatttattt aacttataaa aatattgaag taagattggg
       61 gagattatga atttatggaa ttgaaagtcg atgattttgt aaagaatata aaaagaccat
      121 acttgactgt attgggagta tttgtagttg cagtttcatt attttttgac gcgattatgt
      181 ttttctacgc taaactatat gataaattgc ctatgtattt actggtgttt atggccttta
      241 cagctgtaat tttgattatg atgtacatac aagagaaaaa tgaaaattac aaagttgaaa
      301 aaagatatgt ggttagatat ctcacactta acgttattgt gggatatact ttgccattgc
      361 tttttgtatc tatttacgtt tttggtgtag tcggttttgg atttgatgtt ttcaattatt
      421 gtctaggtat tatcttgatg ttatttattt cttggttagg tttattttta ttttataaaa
      481 acgaatttga tagtgaaaat cctaatcctg cagttaatgc catagcaatt attataaaat
      541 tatttgcttt tggaggaata ttttatataa gtctaatagt tcctgtaaca cagcaagaag
      601 agatttttat agggttaagt atattcatca atataattgt tgatgctctt cttgttaggt
      661 catattttaa ttacgcgtta tataagagta ttaagaaaga tatcgaaaat gaaggtaaga
      721 caatgtaaag gaaagacata aaaaataata ggagtctaac catgataatt tttatttt
//
LOCUS       DAXWFK010000355          774 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.363, whole genome shotgun sequence.
ACCESSION   DAXWFK010000355 DAXWFK010000000
VERSION     DAXWFK010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.363"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            140..667
                     /gene="isaB"
                     /locus_tag="ACGEXS_002802"
     CDS             140..667
                     /gene="isaB"
                     /locus_tag="ACGEXS_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunodominant staphylococcal antigen IsaB"
                     /protein_id="HFF8013743.1"
                     /translation="MNKTSKVCVAATLALGTLIGVTVVENSAPTSKQAQAAITPYYTY
                     NGYIGNNANFILDKNFINAIKYDNVKFNGIKLAKTNTIKKVEKYDQTFKGVSAKGNEA
                     SQLQFVVKNNISLKDIQKAYGKDLKKENGKTKEADSGIFYYQNAKKTLGIWFVVDHNR
                     VVEVTVGHTPYKTSK"
BASE COUNT          309 a           84 c          139 g          242 t
ORIGIN      
        1 tcattgtttt ttgtgtactt caattagtat aacaaaactg ttatgtattt ataaatataa
       61 tcattagtat ttttatggct gaaaaagtta taataaaagt gtaaggaata aaatatttgt
      121 atggaaaaga gagataatta tgaataaaac cagtaaagtt tgtgtagcag caacattagc
      181 attgggcaca ctgattggag taacagttgt tgaaaattcg gcgccaacaa gtaaacaggc
      241 acaggcagca ataaccccat attatactta taatggttat attggtaata atgctaattt
      301 tattctggat aagaatttta ttaacgcgat taagtatgat aatgtgaaat tcaatggtat
      361 taaattagct aaaacgaata cgataaaaaa agtagagaaa tatgatcaaa cttttaaagg
      421 tgtttctgca aaaggaaacg aagcaagtca attgcaattt gtagttaaaa ataatatttc
      481 attaaaagat atccaaaaag cttatggcaa ggacttgaaa aaagaaaatg gtaaaacaaa
      541 ggaagctgat agcggtattt tttactatca aaatgctaaa aagacattag gcatctggtt
      601 tgtcgttgat cataatagag ttgtcgaagt aacagttgga catacaccat acaaaacaag
      661 taaataaaat aatggcatat taaggctaga gtgtgaggag tgataccgca ttctagtctt
      721 ttttattaaa taataacgat tattgcgtct tacatagttg tttgaaattg ataa
//
LOCUS       DAXWFK010000356          772 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.364, whole genome shotgun sequence.
ACCESSION   DAXWFK010000356 DAXWFK010000000
VERSION     DAXWFK010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.364"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(396..587)
                     /locus_tag="ACGEXS_002803"
     CDS             complement(396..587)
                     /locus_tag="ACGEXS_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LSM domain protein"
                     /protein_id="HFF8013744.1"
                     /translation="MNLQSYIGKLVKLTLTNNKILIGKVIDFDDKVDNFDGYNSIEID
                     TGRITYDISENKIKTIVLQ"
     gene            complement(594..>772)
                     /locus_tag="ACGEXS_002804"
     CDS             complement(594..>772)
                     /locus_tag="ACGEXS_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin type 50 domain-containing
                     protein"
                     /protein_id="HFF8013745.1"
                     /translation="IDIPVLFDDEGNFRIKQVIDYKKIIGKSYVNGKYIETKLGKVHY
                     SKTGFHVVPYIKKE"
BASE COUNT          254 a           94 c          101 g          323 t
ORIGIN      
        1 tcttgtttta ttttatatat ttaaaaatgt taatttgtgc tgtatttact ttaatttgac
       61 gacatttgag aagtattatg atggttgtag ttattttatg aaagtaatgt attaacaatc
      121 gaattactaa gtctatattg gtaggaatat cgatgtttag tttaaatgga atgcactatt
      181 taagttttta atatggagat gtttgtgact tttcgatgat taagattttt ataggtgtgc
      241 atcatttcaa ataaactttg tgtttaaaat tgagcttagg aaatcgatag gtttagatga
      301 ggatattgtt gaagttatgt gtcttgtatc cttagttgtt ataaaagcgc aaaaaatagc
      361 accgctttct ctttatctgt gtagaaagga tgctattatt gtaaaacaat agttttaatt
      421 ttattttctg atatatcata tgtaattcta cctgtatcaa tttctatcga attataacca
      481 tcaaaattat caactttatc atcaaagtct atcacttttc caattaatat tttattatta
      541 gtaagcgtta atttgactaa tttgcctatg tatgactgta aattcatatt taatcactcc
      601 tttttaatat acggaactac atgaaaacca gttttagaat aatgaacctt acctaatttc
      661 gtttcaatat atttaccatt cacataagat ttacctatta tctttttata atctataact
      721 tgttttattc taaaattccc ttcatcatca aacaaaactg gaatatcaat aa
//
LOCUS       DAXWFK010000357          755 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.365, whole genome shotgun sequence.
ACCESSION   DAXWFK010000357 DAXWFK010000000
VERSION     DAXWFK010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.365"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..>755)
                     /locus_tag="ACGEXS_002805"
     rRNA            complement(<1..>755)
                     /locus_tag="ACGEXS_002805"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          156 a          228 c          163 g          208 t
ORIGIN      
        1 aaggatgtca agatttggta aggttcttcg cgttgcttcg aattaaacca catgctccac
       61 cgcttgtgcg ggtccccgtc aattcctttg agtttcaacc ttgcggtcgt actccccagg
      121 cggagtgctt aatgcgttag ctgcagcact aaggggcgga aaccccctaa cacttagcac
      181 tcatcgttta cggcgtggac taccagggta tctaatcctg tttgatcccc acgctttcgc
      241 acatcagcgt cagttacaga ccagaaagtc gccttcgcca ctggtgttcc tccatatctc
      301 tgcgcatttc accgctacac atggaattcc actttcctct tctgcactca agttttccag
      361 tttccaatga ccctccacgg ttgagccgtg ggctttcaca tcagacttaa aaaaccgcct
      421 acgcgcgctt tacgcccaat aattccggat aacgcttgcc acctacgtat taccgcggct
      481 gctggcacgt agttagccgt ggctttctga ttaggtaccg tcaagatgtg cacagttact
      541 tacacatatg ttcttcccta ataacagagt tttacgatcc gaagaccttc atcactcacg
      601 cggcgttgct ccgtcaggct ttcgcccatt gcggaagatt ccctactgct gcctcccgta
      661 ggagtctgga ccgtgtctca gttccagtgt ggccgatcac cctctcaggt cggctatgca
      721 tcgttgcctt ggtaagccgt taccttacca actag
//
LOCUS       DAXWFK010000358          755 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.366, whole genome shotgun sequence.
ACCESSION   DAXWFK010000358 DAXWFK010000000
VERSION     DAXWFK010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.366"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..204
                     /locus_tag="ACGEXS_002806"
     CDS             <1..204
                     /locus_tag="ACGEXS_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFF8013746.1"
                     /translation="FCFTIFYYFLFGGLSIMALSMLLTLNPQNIIGFIGWLVMTAGFF
                     LLNMSSIIDKKIYVLSKTNTVEK"
     gene            221..438
                     /locus_tag="ACGEXS_002807"
                     /pseudo
     CDS             221..438
                     /locus_tag="ACGEXS_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020813.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="T7SS effector LXG polymorphic toxin"
     gene            439..>755
                     /locus_tag="ACGEXS_002808"
     CDS             439..>755
                     /locus_tag="ACGEXS_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFF8013747.1"
                     /translation="MLLCESKIINKNPKYRIIKYNDEYLMVDIISTWISLFFPFINWF
                     IPKEYVKISREEFENLNIVKPAKKNVFWPVAGSSALLGVALRKYTHLLDIQLDKKLVI
                     AIC"
BASE COUNT          271 a           91 c          131 g          262 t
ORIGIN      
        1 ttttgtttca caatatttta ttatttttta tttggcggtc tttcaataat ggctctaagt
       61 atgttattaa ctttaaatcc tcaaaatata ataggcttta ttggttggtt ggtaatgact
      121 gcaggtttct ttctgttaaa catgtcatcg attattgaca aaaaaattta tgtattatct
      181 aaaactaaca cggtggaaaa atgatggttt agctggattt actgcaggtt ctatttcggc
      241 aatacttgta tattggacca atcaaaaaaa tgaatttgga ataaaagata aaaacgattg
      301 gataggacat aaactagacg ttggtataga tgctgtagaa aaatctgcag aaaaaacagt
      361 agatggtgtt gaaaatgtca tggtgaagct tcaaaaagta tttctaatca tataagccct
      421 aagaaatgga gctggtaaat gttgctatgc gaatctaaaa tcatcaataa aaacccaaaa
      481 tatagaatta ttaaatataa tgatgaatac ttaatggtcg atataataag cacttggatt
      541 agtttatttt ttccttttat taattggttc atcccaaaag aatacgtcaa aattagtaga
      601 gaagagtttg aaaacttaaa tattgttaaa cctgctaaaa agaatgtttt ttggccagtt
      661 gcaggtagct ctgctttgct gggagttgca ttaagaaaat atacacattt acttgacatt
      721 caacttgata aaaaattagt tattgccata tgttg
//
LOCUS       DAXWFK010000359          728 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.368, whole genome shotgun sequence.
ACCESSION   DAXWFK010000359 DAXWFK010000000
VERSION     DAXWFK010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.368"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..719
                     /locus_tag="ACGEXS_002809"
     CDS             <1..719
                     /locus_tag="ACGEXS_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFF8013748.1"
                     /translation="QILDESKNFGSNSGSFVMYSMKKDKYYIYNEKESRKRYSPDSTY
                     KIYLALFGLDRHIISDKNSRMSWNHNHYPFDSWNKDQDLNTAIQNSVNWYFERISNQI
                     SKNYTSDQLKQLNYGNKNLGSYKAYWLEDSLKISNLEQVIVLKNMMEQNNHFSKNEKK
                     QLSSSLLIRKNENYELYGKTGTGIVNGKYNNGWFVGYVITNHDKYYFSTHLSDEKASG
                     ENAKLINEKILKEMGVLNGQ"
BASE COUNT          301 a           77 c          120 g          230 t
ORIGIN      
        1 accaaatttt agatgaaagt aaaaattttg gttcaaatag tggatctttc gttatgtaca
       61 gcatgaagaa agacaaatac tatatttata atgaaaaaga aagtagaaaa agatattctc
      121 ctgattcaac ttacaaaatt tatttagcac tgtttggact tgaccgtcat attataagtg
      181 ataaaaattc acgtatgtca tggaatcata atcattatcc tttcgattct tggaataagg
      241 atcaagattt aaatacggca atacaaaact cagttaattg gtacttcgaa cgtattagta
      301 atcaaatatc aaagaactat acttctgatc aactcaagca attaaattat ggtaataaaa
      361 atttgggaag ttataaagcc tattggttgg aagatagttt gaaaatatct aatcttgaac
      421 aagtaatagt tttgaaaaat atgatggaac aaaataatca ttttagtaaa aatgaaaaga
      481 aacaattatc ttcttcgtta ttgattagaa aaaatgaaaa ttatgaattg tatgggaaaa
      541 caggtacagg tatagtaaac gggaagtaca ataatgggtg gtttgtaggt tacgtaatta
      601 caaatcatga taagtattat ttttctacac atttatcaga tgaaaaagcc tctggtgaaa
      661 atgctaaatt aataaatgaa aaaatattaa aagaaatggg tgttttaaat ggccaataag
      721 caagttga
//
LOCUS       DAXWFK010000360          727 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.369, whole genome shotgun sequence.
ACCESSION   DAXWFK010000360 DAXWFK010000000
VERSION     DAXWFK010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.369"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            43..168
                     /locus_tag="ACGEXS_002810"
     CDS             43..168
                     /locus_tag="ACGEXS_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013749.1"
                     /translation="MRYIHFSIITLITGIIMHITMYFVPFESRKMPLFMTIIFTI"
     gene            168..416
                     /locus_tag="ACGEXS_002811"
                     /pseudo
     CDS             168..416
                     /locus_tag="ACGEXS_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669339.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAR2788 family putative toxin"
     gene            430..>727
                     /locus_tag="ACGEXS_002812"
     CDS             430..>727
                     /locus_tag="ACGEXS_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013750.1"
                     /translation="MCFDINNLDIKKLNFRKVKNAIHLSLSGDKYQFLEDGKTINNTY
                     FLAVYDNAINIFEDLFSPSDSINLVHVVYVYSEPYKKTNVIKKFTSLTIDELYYF"
BASE COUNT          286 a           75 c          118 g          248 t
ORIGIN      
        1 cttggaatta attatttaat aagttgtata gcaggtggcg atatgagata tatacacttt
       61 tctattatta cattaattac aggtatcatt atgcatatta caatgtactt tgtaccattt
      121 gaatctcgga aaatgccact atttatgacg ataattttta caatctaaaa gcagtaaaga
      181 ctcataatat aaccaaaaac attaataaaa gtttgttaaa tgataagggg atgagtattg
      241 gtgaatttac tcaaaaggtt taagggaaaa atcctacctg gagagattca aaaactaaat
      301 ggactatttc caaaaataaa gggcaatcac atggtggttc ttattggaag ttaataaata
      361 acaaaggaaa aagaatagct tctttaacta aagaaggaaa aatcttaagg gaataaggtg
      421 ttgtaaatta tgtgctttga tataaataat ttggatataa aaaagttgaa ttttagaaag
      481 gtaaaaaacg caattcattt aagtttaagt ggcgataaat accagttttt ggaggacggt
      541 aaaactatca ataatactta ttttttagct gtatatgata atgcaataaa catttttgaa
      601 gatttgtttt cgccttctga ttctataaat ttggttcatg tagtatatgt ttatagtgaa
      661 ccttataaga aaacaaatgt tataaaaaaa tttacatcgt taacaataga tgaattgtat
      721 tacttta
//
LOCUS       DAXWFK010000361          718 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.370, whole genome shotgun sequence.
ACCESSION   DAXWFK010000361 DAXWFK010000000
VERSION     DAXWFK010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.370"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            123..331
                     /locus_tag="ACGEXS_002813"
                     /pseudo
     CDS             123..331
                     /locus_tag="ACGEXS_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(349..654)
                     /locus_tag="ACGEXS_002814"
                     /pseudo
     CDS             complement(349..654)
                     /locus_tag="ACGEXS_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500634.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YolD-like family protein"
BASE COUNT          264 a          109 c          107 g          238 t
ORIGIN      
        1 aaaaaataag acactttgcc caacttgcac ataaatgtaa aattcaataa aatgaatttt
       61 ctgtgttggg tcccttctta taatttaata aataccacta aactaaatta acgaggtgcc
      121 ttatgtataa aaattataac atgccccaac tacactacca atagaaactt ctgttagaat
      181 ccctcaaaat gatatttcac gatatgttaa tgaaattgtt gaaacaatac ctgatagcga
      241 attcgatgaa ttcagacatc atcgtggcgc aacatcctat catccaaaaa tgatgttaaa
      301 aatcatctta tatgcatata ctcaatctgt ttaattatgt tcaaagcatt aaggtaacaa
      361 gacaatatct aagatatcaa agatagaaat tttttgacgt tgttgctgat tgtaaacata
      421 accatcaatt tcataattaa tagcatcaat acgataaatg gttaagcgta ctgaatctac
      481 aaagccatta ttataaaatt taacttctac aggttgggca tattgtagcg cctcgtgtag
      541 ccgaatgttt agctcagcca attgatcatc tgataataca ggacgtgtaa ttttgttttg
      601 gtcgataatg tattgttgaa tcgtttcgaa ttgttcgggt aatgttgcaa aaggttatat
      661 atatgttgaa gaatatagac tattttgttt aaaatgaatc atattttaag aaaaaatg
//
LOCUS       DAXWFK010000362          704 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.371, whole genome shotgun sequence.
ACCESSION   DAXWFK010000362 DAXWFK010000000
VERSION     DAXWFK010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.371"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..566
                     /locus_tag="ACGEXS_002815"
     CDS             <1..566
                     /locus_tag="ACGEXS_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4888 domain-containing protein"
                     /protein_id="HFF8013751.1"
                     /translation="NTKFLGKTLVASALVLTTLGTGLHSSYLGLDTNKVVKTAKAEEK
                     MTDGQLWKKVKDSLHDSDIILSNEYETINVTYLLSNGYYSSVSAPGNDDGGHLTQSID
                     FKGLKQIDLTKENVYDDFNKKLDAKNTWNSLTEKLKGLGLLQNGQKVSIYSSDSSSPV
                     SGKVGEGVTSGGENTLTKRFINKITID"
BASE COUNT          279 a          112 c          104 g          209 t
ORIGIN      
        1 tgaatacaaa atttttaggt aaaacattag tagcaagtgc tttagtatta acaacattag
       61 gaacaggctt acattcttca tacttaggat tagatacaaa taaagttgtt aaaacggcta
      121 aagcagaaga aaaaatgaca gatggtcaat tatggaaaaa agttaaagat tcattacatg
      181 attctgatat tattttaagt aacgagtacg aaacaatcaa cgttacttat ttattaagca
      241 atggatatta tagcagcgtt tctgctcctg gaaatgatga tggaggccat ctaacacaat
      301 ctatagactt taaaggatta aaacaaattg atttaactaa ggaaaatgtc tacgatgact
      361 ttaacaaaaa gctagatgct aaaaatacat ggaattctct aaccgagaag ttaaaaggtc
      421 ttggtttact acaaaatggc caaaaagtat ctatttattc ttcagatagc tcttctccag
      481 tctcgggtaa agtgggagaa ggagtaacta gtggtggcga aaatactcta actaaacgct
      541 tcatcaacaa aatcacaatt gattaaacct taaactcaaa tttccttaat tcataaaaca
      601 ttaataacta accctttctt acaaaccacc tagcccataa tatattaaaa cgtcattttt
      661 atttcaatta gcggaaaata aaaaaaccat tccgcattaa cttt
//
LOCUS       DAXWFK010000363          676 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.373, whole genome shotgun sequence.
ACCESSION   DAXWFK010000363 DAXWFK010000000
VERSION     DAXWFK010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.373"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..612)
                     /locus_tag="ACGEXS_002816"
     CDS             complement(<1..612)
                     /locus_tag="ACGEXS_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HFF8013752.1"
                     /translation="MSQNTNHSYYHQNQHAQSISKVWLYFMYYWIIFGIGCYLGQFLP
                     LSWRQPLSFGLLIIILATLVFERARRFGLIISHIYAVVIGLLSYATFTTYLQNLGPDI
                     FYKNIALAIFAFIAFGIIGYFFVGDASSIGKYLFVTLITLIIASLIGIFLQNPIFYTI
                     ITVVSLLLFLLYTLYDFNRLKRGDYSPREMGFNLFINLLNIIKD"
BASE COUNT          269 a          110 c           89 g          208 t
ORIGIN      
        1 atccttaata atattcaaca aattaataaa tagattaaat cccatttctc ttggtgaata
       61 gtcacctctt tttaaacgat taaaatcata caaagtataa agtagaaata acaacagact
      121 aacgacggta ataatagtgt aaaaaatagg attttgaaga aaaataccaa ttagactcgc
      181 aataattaat gttattaatg taacgaataa atatttgcct atactcgatg catctccaac
      241 gaagaaataa ccaataatac caaatgctat aaatgcaaaa attgctaatg cgatattttt
      301 atagaaaata tctggtccta aattttgtaa atacgtggta aacgttgcgt atgacaataa
      361 gccaatcact acagcgtaaa tatgtgaaat aattaaaccg aaccgtctcg ctctttcaaa
      421 aacaagtgtt gctaaaataa taatcagtaa tccaaatgac aagggttgtc gccaacttaa
      481 tggtaaaaac tgacctagat agcatcctat gccaaatata atccaataat acataaaata
      541 aagccacact ttacttattg attgagcatg ctggttttga tgataatatg aatgatttgt
      601 attttgcgac aaaataattc ctccttctac cgtcgtttta tgcgtcaaat tttacagtaa
      661 attttgcttc atcaaa
//
LOCUS       DAXWFK010000364          655 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.376, whole genome shotgun sequence.
ACCESSION   DAXWFK010000364 DAXWFK010000000
VERSION     DAXWFK010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.376"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..>655
                     /gene="lpl7"
                     /locus_tag="ACGEXS_002817"
     CDS             <1..>655
                     /gene="lpl7"
                     /locus_tag="ACGEXS_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl7"
                     /protein_id="HFF8013753.1"
                     /translation="KETQIKKSFAKTLDVYPTKNLEDFYDKEGYRDGEFKKGDKGKWV
                     IRSEMTTELKNENMVSKGMVIRLNRNSRTCTGEYFVRIVKEDSEGKVYSDERKYPVKM
                     ENNKIITLKPIDDEKVKKEIEEFKFFVQYGNFKELENYKDGEVTYNPEAPIYSAQYQL
                     KNSDYNVEQLRKRYNITTKKAPKLLLKGSGNLKGSSVGYKNIEFTFVENKEENIYFTD
                     "
BASE COUNT          293 a           73 c          126 g          163 t
ORIGIN      
        1 gaaagaaaca cagatcaaaa agagctttgc gaaaacatta gatgtatacc ctacgaaaaa
       61 tctagaagat ttttatgaca aagaaggata tcgagatggc gaatttaaaa agggtgacaa
      121 agggaagtgg gttattagat ctgaaatgac aacagaactg aaaaatgaaa atatggtatc
      181 taaaggtatg gtcatacgtt taaatagaaa tagtagaaca tgcactggtg aatattttgt
      241 caggatagtt aaagaagaca gtgagggcaa ggtatatagt gatgaacgaa aatatccagt
      301 gaaaatggaa aataataaaa tcattacatt aaaaccaatc gatgatgaaa aagtaaaaaa
      361 agaaattgaa gaatttaaat tcttcgtaca atacgggaat ttcaaagaat tggaaaacta
      421 taaagacgga gaagtgacat ataacccaga agcaccaata tactctgcac aatatcaatt
      481 gaaaaatagt gattataatg tagaacaact acgtaagcga tataatataa cgacgaaaaa
      541 agcgcctaaa ttattattga agggttcagg taatttaaaa ggctcatcag ttggatataa
      601 aaatattgaa tttacctttg ttgaaaataa ggaagaaaat atttacttca cagat
//
LOCUS       DAXWFK010000365          648 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.377, whole genome shotgun sequence.
ACCESSION   DAXWFK010000365 DAXWFK010000000
VERSION     DAXWFK010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.377"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            123..>648
                     /locus_tag="ACGEXS_002818"
     CDS             123..>648
                     /locus_tag="ACGEXS_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFF8013754.1"
                     /translation="MYKNYNLIQLTLPIETSVRIPQIDISRYVNEIVETIPDSEFDKF
                     RHHRGATSYHPKMMLKITLYAYTQSVFSGRRIEKLLHDSIRMMWLAQNQTPSYKTINR
                     IRVNPNTDALIESLFIQFHSQCLKQNLIDGTKVEANANRYTFVWKKSIQNHESKLNEN
                     SKTLYRDLVEEKIIP"
BASE COUNT          248 a          100 c           91 g          209 t
ORIGIN      
        1 aaaaaataag acactttgcc aaacttgcac ataaatgttt aattcaataa tttgaatttt
       61 ctgtgttggg tcccttcgta taatttaata aataccacta aactaaatta atgaagtgcc
      121 ttatgtataa aaattataac ttgatccaac ttacactacc aatagaaact tctgttagaa
      181 ttcctcaaat tgatatttcg cgatatgtta atgaaattgt tgaaacgata cctgatagcg
      241 aattcgataa attcagacat catcgtggcg caacatccta tcatccaaaa atgatgttaa
      301 aaatcacctt atatgcatat actcaatctg tattttctgg tcgtagaata gagaaattac
      361 ttcatgacag tattcgaatg atgtggttag ctcaaaatca aacaccttct tataaaacta
      421 ttaatcgtat tagagtgaat cctaatactg atgcgttaat tgaatcttta tttatccagt
      481 ttcatagtca atgtttaaag caaaatctta ttgatggtac aaaagtagaa gctaatgcca
      541 ataggtatac atttgtgtgg aagaaaagta ttcaaaatca cgaatcgaaa ttgaacgaaa
      601 attcaaaaac attatatcgt gacttagttg aagaaaaaat aataccag
//
LOCUS       DAXWFK010000366          639 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.378, whole genome shotgun sequence.
ACCESSION   DAXWFK010000366 DAXWFK010000000
VERSION     DAXWFK010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.378"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(328..>639)
                     /locus_tag="ACGEXS_002819"
     CDS             complement(328..>639)
                     /locus_tag="ACGEXS_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFF8013755.1"
                     /translation="NYRLSNVDTMKVTLYSNGSNYDKEALLINKDEFCPLRKITLDNK
                     LDSQRVMEIDSLAAIINLVKQGKGKALLPMTFENKRDIVQDISKIFEVNYYTYNHIMH
                     H"
BASE COUNT          221 a          101 c           75 g          242 t
ORIGIN      
        1 taacaaaaat actgtttgtg tataacaata aattgagata ctcaataatt tgataatgtg
       61 aaattgatac ttttcaaaat agttgaatat gacaattaat gattagtttt gcaatggtgc
      121 tataatcaat atttagcagt tatcattatg cttgaaaggg tggtaaaggc ggagaagtca
      181 tccacttttt cctatactac aaggaccttt actttaaaac aacgaattta caaaataaaa
      241 ggcataattt cttttctgtc acatcgctca ttctaactac tagactttta aaatcgttcc
      301 tgtaatttaa actcaatcaa ctttcaatta atgatgcata atatgattat aagtatagta
      361 gttaacttca aatatcttag aaatgtcctg tactatatct cttttattct caaaagtcat
      421 aggtaataaa gcctttcctt ttccttgttt aactaagttt attattgctg ccaatgaatc
      481 aatttccatt acccgttgtg aatcaagctt attatctaaa gttattttcc ttaaaggaca
      541 aaactcatct ttatttatta gtaaagcctc tttatcataa ttacttccat tactgtaaag
      601 cgttactttc atagtatcaa cattactaag tctataatt
//
LOCUS       DAXWFK010000367          637 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.379, whole genome shotgun sequence.
ACCESSION   DAXWFK010000367 DAXWFK010000000
VERSION     DAXWFK010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.379"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..288
                     /gene="lpl4"
                     /locus_tag="ACGEXS_002820"
     CDS             <1..288
                     /gene="lpl4"
                     /locus_tag="ACGEXS_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl4"
                     /protein_id="HFF8013756.1"
                     /translation="KNYSNGDVSINENVPSYDVKYKMSNKDENVKQLRSRYNIPTDKS
                     PVLKMHIDGNLKGSSVGDRKLEIDFSKRENSHLSVIDSLDYQPAKTNKDDE"
     gene            307..>637
                     /gene="lpl5"
                     /locus_tag="ACGEXS_002821"
     CDS             307..>637
                     /gene="lpl5"
                     /locus_tag="ACGEXS_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl5"
                     /protein_id="HFF8013757.1"
                     /translation="MKSIKRIGLCISLLILSIFVTSCDGDNKITGDSKETQIKKSFSK
                     TLDMYPIKNLEDLYDKEGYRDGEFKKGDKGMWTIYTDFAKGNKSDELDDEGMVLNLDR
                     NTRTAKGY"
BASE COUNT          250 a           69 c          136 g          182 t
ORIGIN      
        1 aaaaactaca gcaatggtga tgtctcaatt aatgagaatg ttccaagtta tgacgtgaaa
       61 tataaaatga gtaataaaga tgaaaatgtt aagcaattaa gaagtcgtta taatattcct
      121 actgataaat caccggtatt aaaaatgcat attgatggta atttgaaagg aagttccgtt
      181 ggagatagga agttagaaat tgatttttcg aaacgtgaaa atagccattt atcagtaata
      241 gattctttgg attaccagcc agcgaaaact aataaagatg atgaataatg aggatggtgt
      301 gtaacaatga agtctataaa aaggattgga ttgtgcataa gtctgttgat tttaagcatt
      361 tttgtaacat cttgcgatgg tgataataag atcactggag attcaaaaga aacacaaatc
      421 aaaaagagct tttcgaaaac gttagatatg tatccaatta agaatctcga ggacttatat
      481 gataaagaag gataccgtga tggcgaattt aaaaagggcg acaaaggaat gtggactata
      541 tatacagatt ttgctaaagg caataaatca gacgaattgg atgatgaagg tatggtttta
      601 aatctggata gaaatactcg aacggctaag ggatatt
//
LOCUS       DAXWFK010000368          631 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.380, whole genome shotgun sequence.
ACCESSION   DAXWFK010000368 DAXWFK010000000
VERSION     DAXWFK010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.380"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..>631
                     /locus_tag="ACGEXS_002822"
     CDS             <1..>631
                     /locus_tag="ACGEXS_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6119 family protein"
                     /protein_id="HFF8013758.1"
                     /translation="LIKNVYLDTEEDDNLFSESGHHFDAILFLKEDTTLQNNVYIIPF
                     GQAYHDINNLIDYDFGIDFAERAIKNEDIVNKNVNFFQQNRLKEIVNYRRNSVDYVRP
                     SESYISVQGHPQNPQIFGKTMTCGTSISLRVPNRKQQFIDKISVIIKEINAIINLPQK
                     ISEFPRIVTLKDLNKIEVLDTLLLKKLSNSSTTENISIDISRFLELSNMI"
BASE COUNT          263 a           77 c           88 g          203 t
ORIGIN      
        1 attaataaag aatgtatacc tagatactga agaagatgac aatttatttt cagaatccgg
       61 tcatcatttt gatgcaatat tatttctcaa agaagataca acattacaaa ataatgtata
      121 tattatacct tttggacaag catatcatga tataaataat ttgattgatt atgacttcgg
      181 aattgatttt gcagaaagag caatcaaaaa tgaagacata gttaataaaa atgttaattt
      241 ttttcaacaa aacaggctta aagagattgt taattataga aggaatagtg tagattacgt
      301 tagaccttca gaatcttata tatcagtcca aggacatcca cagaatcctc aaatttttgg
      361 aaaaacaatg acttgtggta caagtatttc attgcgtgta ccgaatagaa agcagcaatt
      421 catagataaa attagtgtga taatcaaaga aataaacgct attattaatc ttcctcaaaa
      481 aattagtgaa tttcctagaa tagtaacttt aaaagacttg aataaaatag aagtattaga
      541 tactttattg ctaaaaaaac tatcgaattc ttcaactaca gaaaatatat ctatagatat
      601 atcaagattt ttagaactaa gtaatatgat a
//
LOCUS       DAXWFK010000369          629 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.381, whole genome shotgun sequence.
ACCESSION   DAXWFK010000369 DAXWFK010000000
VERSION     DAXWFK010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.381"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            314..418
                     /locus_tag="ACGEXS_002823"
     CDS             314..418
                     /locus_tag="ACGEXS_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013759.1"
                     /translation="MFKVNYSILSYYPEYNIAVSWQRLREGKTIKNKI"
     gene            547..>629
                     /locus_tag="ACGEXS_002824"
     CDS             547..>629
                     /locus_tag="ACGEXS_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013760.1"
                     /translation="MLTLIKLKEDEQVIIYEYIPEDDISNG"
BASE COUNT          244 a           74 c          106 g          205 t
ORIGIN      
        1 atgataaaaa agattggaat ggatcgtctt gaaatgctcc cttcaaagtt ttcatttttt
       61 caatgtcgac ttcgaagggg gcattttcat taaattgtta tagcttttta tatttgtata
      121 atgaacatat aagtttaaga aggtgcgagt gaaggaaata aaaaagctca aatgtaccaa
      181 attgttaatc ttaataaatc tctactttat aaagattgaa tggacattcg agcgttaatc
      241 agtcaggagg gactttccct cctacaattt aataataata cttgcttcac cactatacaa
      301 ggagtgagtt gttatgttca aagtgaatta ttcgatttta agttattatc cagaatataa
      361 tatcgcagta agttggcaac gtttaagaga aggaaaaaca ataaaaaaca agatttaata
      421 ctgctgcgtc atgaggcgct tgaacattat ttgatgaata agtataattt caactatgat
      481 tatgcacata aaattgtatc aaaaaaatac gattattcaa tttttataaa aaagaaggtg
      541 gattaaatgc ttacattaat aaaattgaaa gaagatgaac aggttataat atatgaatat
      601 atacctgaag atgatataag taacggtaa
//
LOCUS       DAXWFK010000370          625 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.382, whole genome shotgun sequence.
ACCESSION   DAXWFK010000370 DAXWFK010000000
VERSION     DAXWFK010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.382"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..291
                     /locus_tag="ACGEXS_002825"
     CDS             <1..291
                     /locus_tag="ACGEXS_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HFF8013761.1"
                     /translation="ERITLYLKHNTKEPNTIKTVHFTSLKRGPMGDAVIEGYINENKE
                     DDFVAYGSPEHNYQFGGSLIKSKNLSTLLKPVHQTKSPDEIKKELESKKNDR"
BASE COUNT          233 a           88 c           95 g          209 t
ORIGIN      
        1 gagcgtatta cactctatct taagcataat actaaagaac ccaataccat caaaactgta
       61 catttcacaa gtttaaaaag aggacctatg ggcgatgcag taattgaagg ttacataaat
      121 gaaaataaag aagatgattt tgttgcatat gggtctccag aacataatta tcaatttggt
      181 ggaagtttaa tcaaaagtaa aaatttaagc acgttattaa aaccagtaca tcaaaccaaa
      241 tcacctgatg agataaaaaa ggaattagag tctaaaaaga acgaccgcta aaactatgcg
      301 gccgttttaa ttttgttttc aagttaatca tacttacata taaaaatgaa aagttgaatt
      361 tttagtacaa tttccactgt atttatctgt taaccacttt atacccgcaa tatttcgttg
      421 atcttatata aacacttttg gctgcttttc cttgacctag attaaaatta tagattttct
      481 ttgtaagagt gtgttttgta gtatcataaa tatgtaaact gtataaaatt ccaccacatt
      541 ttaggagctg gtgatagatg tcgaaaaaga aaattttaat attaactagt attatgttaa
      601 tcatattaat tagtgttgca ggtat
//
LOCUS       DAXWFK010000371          616 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.383, whole genome shotgun sequence.
ACCESSION   DAXWFK010000371 DAXWFK010000000
VERSION     DAXWFK010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.383"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..>616)
                     /locus_tag="ACGEXS_002826"
     CDS             complement(<1..>616)
                     /locus_tag="ACGEXS_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HFF8013762.1"
                     /translation="RDVTLSIYLPESYNQLVKYNVILCFDGLDFLRFGRIQRTYESLI
                     KEARIDDAIIVGFHYEDVDKRREEFHPQGSRSHLTIQSVGKEILPFIDSTFSTLKVGN
                     ARLLVGDSLAGSIALLTALTYPTIFSRVAMLSPHSDEKVLDKLNQCANKEQLTIWHVI
                     GLDEKDFTLPTNGKRADFLTPNRELAEQIKKYNITYYYDEFDGG"
BASE COUNT          201 a          120 c           86 g          209 t
ORIGIN      
        1 tgaccaccat caaattcatc gtaataataa gttatattat atttcttaat ttgttcagct
       61 aattctctat tcggtgttaa gaaatcggca cgcttaccat ttgttggtaa agtaaaatct
      121 ttttcatcta gaccaatgac atgccaaatt gtcaattgtt ctttatttgc acattgattt
      181 agcttatcta atactttttc atctgaatgt ggacttaaca ttgctacacg actaaaaatc
      241 gttggatagg tcaacgccgt taataaggca atactacccg ctaaactatc ccctactaat
      301 aaccttgcat tacctacttt cagtgtagaa aacgtcgagt caataaatgg caatatttct
      361 ttaccgactg attgaatagt taaatgagaa cgacttcctt gtggatgaaa ttcctctcta
      421 cgcttatcaa cgtcttcata atggaatcca acaatgatcg catcatcaat acgcgcttct
      481 ttgattaacg attcatatgt acgttgtatt ctcccgaaac gtaaaaaatc taatccgtca
      541 aagcaaagaa tgacattata tttaacaagt tgattataag attctggtaa ataaatactt
      601 aacgtgacat ctctat
//
LOCUS       DAXWFK010000372          611 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.384, whole genome shotgun sequence.
ACCESSION   DAXWFK010000372 DAXWFK010000000
VERSION     DAXWFK010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.384"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            1..611
                     /locus_tag="ACGEXS_002827"
                     /pseudo
     CDS             1..611
                     /locus_tag="ACGEXS_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          248 a           85 c           94 g          184 t
ORIGIN      
        1 aagaagttca aaaagaaatt tgatgattat tccgaaagaa aaaataaata tgaagaacaa
       61 aaatcgattc ttaaagatag aaatagtttt tctaaaactg atcatgatgc aacttttatg
      121 agaatgaagg aagaccatat gaaaaatggc caacttaagc caggatacaa tttacaaata
      181 gcgacaaatt ctcaatttgt tttatcctat gacctatttc aaaacccgac agatactaga
      241 actttaattc catttttaac aatgattcaa aataccttcg gttatttgta gctgatgcag
      301 gttatggtag tgagcaaaac tatatggcca ttatagatga ttttaataaa acgccactta
      361 ttacgtatgg catgtttatt aaagataaaa ctagaaagtt taaaagtgac attttcaaca
      421 ctcaaaattg gaaatatgac gaacttaatg atgaatttat atgtcctaat aacaaaagaa
      481 taggttttaa aagatatgca taccgtaatg atagatatgg ttttaaacga gacttcaaac
      541 tatatgaatg cgatgactgt tcagcatgtt ctttgaggca acaatgcatg aaaccaaatt
      601 caaaatccaa t
//
LOCUS       DAXWFK010000373          606 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.385, whole genome shotgun sequence.
ACCESSION   DAXWFK010000373 DAXWFK010000000
VERSION     DAXWFK010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.385"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(310..510)
                     /locus_tag="ACGEXS_002828"
     CDS             complement(310..510)
                     /locus_tag="ACGEXS_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFF8013763.1"
                     /translation="MNNGTVKWFNAEKGFGFIEREDGSDVFVHFSAIAEDGYKSLEEG
                     QKVEFDIVEGDRGEQAANVVKM"
BASE COUNT          195 a          112 c           79 g          220 t
ORIGIN      
        1 tattattgtt aaacattttg agtgtttaac attacttctt gttatcgcca tatacagact
       61 ttaattctcg tcattattaa agttctgtat tggcgctttt ttatattcaa aatcaaaagt
      121 cggacagatg aaaagtaaaa aacttttcca tcagttcgat ttattttaga accattatat
      181 attgttaaat ctccaactaa atttattgga caaaaaaaat aacggctagt gcttttacca
      241 caccaactgt tatcgattta aagcataaaa aaacagccct atagtaagga ctgtttgaat
      301 aagttaaaat tacattttaa ctacgtttgc agcttgctcg ccacggtcgc cttcaacgat
      361 gtcgaattca actttttggc cttcttctaa tgatttgtat ccatcttcag cgattgctga
      421 gaagtgtacg aatacgtcgc taccatcttc tctttcgatg aaaccaaaac ctttttctgc
      481 attaaaccat ttaactgtac cgttattcat attgaatacc tccgtcgtgc ttttgcacta
      541 aatattttgt aacaaattca taaatgaaaa ggagaatatt ctatataaat aactcacatt
      601 tttctt
//
LOCUS       DAXWFK010000374          601 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.386, whole genome shotgun sequence.
ACCESSION   DAXWFK010000374 DAXWFK010000000
VERSION     DAXWFK010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.386"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            179..553
                     /locus_tag="ACGEXS_002829"
     CDS             179..553
                     /locus_tag="ACGEXS_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4467 domain-containing protein"
                     /protein_id="HFF8013764.1"
                     /translation="MKRILVVFLMLAIILAGCSNKGEKYQKDIDKVYKEQNQMNKIAS
                     KVQNTIKTDIKQEDSNTHVYKDGKVIVIGIQLYKDREKMYYFAYEIKDGKAEINREID
                     PIKYMKDHKADYEDENVEVEKD"
BASE COUNT          267 a           60 c          101 g          173 t
ORIGIN      
        1 atagatgttt atattaagaa gactttaata tcataattaa tcaaaatctt gcgtcaaaca
       61 tttaaacgaa ttttaagttg ataacaattg gcaaaacatt gttcaaacat cacaatgata
      121 aagcatatta tcagtattgt agtgtgtgga aaatgacagc catctaagga gaaaaatgat
      181 gaaaagaata ttggtagtat ttttaatgtt agcaattata ttggcaggtt gttctaataa
      241 aggtgaaaag tatcaaaaag atattgataa agtgtacaaa gaacagaatc aaatgaataa
      301 aattgcctcg aaagtacaaa acactattaa aacagacatt aaacaagaag acagtaatac
      361 acatgtttat aaagatggta aagtcattgt tattggtatt caattatata aagatcgtga
      421 aaaaatgtat tatttcgcat atgaaataaa agatggtaag gcagagatta atagagaaat
      481 agacccaatt aagtatatga aagaccataa agcagattat gaagatgaaa atgtagaagt
      541 ggaaaaagat taacataggc tttctttaaa acttttaacc agtttcaaaa agtaaatgag
      601 c
//
LOCUS       DAXWFK010000375          594 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.388, whole genome shotgun sequence.
ACCESSION   DAXWFK010000375 DAXWFK010000000
VERSION     DAXWFK010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.388"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            23..>594
                     /locus_tag="ACGEXS_002830"
     CDS             23..>594
                     /locus_tag="ACGEXS_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFF8013765.1"
                     /translation="MNLDWYYTFVILAKTLNYRLASEEINLTIPSIHKQIKNLEQHLN
                     VKLFETYKNQIILTEDGHTFLPIAQSFIEQYESGIKHIQLKKTMFQSKLNVVVSSYIA
                     TFIMPKFLKSFFNEHPFIDVSLHVKNENIEKDINNHTYDIGISRNQPKLREVHSEKVC
                     EGKIVLIAPNKENNHLLTEASLFEKYKIIS"
BASE COUNT          228 a           88 c           83 g          195 t
ORIGIN      
        1 cttttttgaa agaggtaagt tcatgaattt agattggtac tatacatttg tcattttagc
       61 gaagacgtta aactatcgat tagctagtga agaaattaat ctcactatac cttctataca
      121 taagcaaatt aaaaatttag aacagcatct caatgtgaaa ctatttgaaa cttataaaaa
      181 tcaaattatc ttgactgaag atggtcatac atttcttcca attgcgcaaa gttttattga
      241 acaatatgaa agtggcatca aacatatcca acttaaaaaa acgatgtttc aatcgaaatt
      301 aaatgttgtc gtgtcttcat atatcgcgac attcattatg cccaaatttt taaaatcttt
      361 ctttaacgag catcctttta tcgatgtttc tcttcatgtc aaaaatgaaa atattgaaaa
      421 agatattaat aatcatacgt atgacatcgg gattagcaga aatcaaccta aactaagaga
      481 agttcattct gaaaaagtat gcgaaggtaa aattgtattg attgctccca ataaagagaa
      541 taatcatctt ctaactgagg catctttatt tgaaaaatac aaaataataa gtga
//
LOCUS       DAXWFK010000376          589 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.389, whole genome shotgun sequence.
ACCESSION   DAXWFK010000376 DAXWFK010000000
VERSION     DAXWFK010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.389"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            157..576
                     /gene="fosB"
                     /locus_tag="ACGEXS_002831"
     CDS             157..576
                     /gene="fosB"
                     /locus_tag="ACGEXS_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FosB/FosD family fosfomycin resistance
                     bacillithiol transferase"
                     /protein_id="HFF8013766.1"
                     /translation="MLKSINHICFSVRNLNDSIHFYRDILLGKLLLTGKKTAYFELAG
                     LWIALNEEKDIPRNEIHFSYTHIAFTIDDSEFKYWHQRLKDNNVNILEGRVRDIRDRQ
                     SIYFTDPDGHKLELHTGTLENRLNYYKEAKPHMTFYK"
BASE COUNT          226 a           80 c           93 g          190 t
ORIGIN      
        1 agttgcgtag tcaactgtat accttttgcc tttaacttaa gttattagag cctcttatgc
       61 agttgcgcag atcatcgtat aaaaattaat gacgtcattt caaaaatcga tacaaaaata
      121 atttattata aaaattctaa gaaagaagtg aagcagatgt taaaatctat taatcatata
      181 tgcttttcag tcagaaattt aaacgattca atacattttt atagagatat tttacttggg
      241 aaattgctat tgactggtaa aaaaactgct tattttgagc ttgcaggcct atggattgct
      301 ttaaatgaag aaaaagatat accacgtaat gaaattcact tttcatatac acatatagct
      361 ttcactatag atgacagcga atttaaatat tggcatcaga ggttaaaaga taataacgtg
      421 aatattttag aaggaagagt tagagatatt agagatagac aatcaattta ctttaccgac
      481 cctgatggtc ataagctaga attacatact ggcacacttg agaacagatt aaattattat
      541 aaagaggcta aaccacatat gacattttac aaataaggtg tcattataa
//
LOCUS       DAXWFK010000377          588 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.390, whole genome shotgun sequence.
ACCESSION   DAXWFK010000377 DAXWFK010000000
VERSION     DAXWFK010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.390"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            205..>588
                     /locus_tag="ACGEXS_002832"
     CDS             205..>588
                     /locus_tag="ACGEXS_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013767.1"
                     /translation="MKTIQLHKTTLILIAILLFYTFMGILLPLMHDDLQWFSNYNTDI
                     LKVGFASLNGRYIGNIFEIIAVHVSWLRWLSYGLISMGIIWMIMHITRCKAWTSYYLL
                     AFSLMLILPSAIYADTYGWFAGFYNY"
BASE COUNT          199 a           71 c          105 g          213 t
ORIGIN      
        1 tgaatagaca agtgtaatca ttcgtcatag aaaaagacga ggggcgattt agataaattg
       61 ttgaaagtaa tattaaatac cttgtttatc taatttgatt gaggaaacat gcataggtga
      121 aaattagaaa tacattaaaa atattgaatt aatttttcga cctacaagtt gaggaaaatt
      181 taactagatt tgaggattag caaagtgaaa acaattcaat tacacaagac aactttgata
      241 ttaattgcga tattattgtt ttatactttt atgggtatct tactaccact catgcacgat
      301 gatttacaat ggtttagcaa ttataatact gatattttaa aagtaggatt tgcatcactt
      361 aatggtcgct atatcggaaa tatctttgaa attatagctg tacatgtaag ctggttacgt
      421 tggctttcat atggcctcat tagtatgggc atcatttgga tgattatgca cattacacgt
      481 tgtaaagcgt ggacaagcta ttatttgtta gcattttctt taatgttaat tttaccaagt
      541 gctatttatg cagatacata tggctggttt gcaggatttt ataattat
//
LOCUS       DAXWFK010000378          585 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.391, whole genome shotgun sequence.
ACCESSION   DAXWFK010000378 DAXWFK010000000
VERSION     DAXWFK010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.391"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..326)
                     /locus_tag="ACGEXS_002833"
     CDS             complement(<1..326)
                     /locus_tag="ACGEXS_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFF8013768.1"
                     /translation="METLLCESKVINKNPKYRIIKYDSEYLMIDLASNWIVFFFPFIN
                     WLIPKTYVKITKNDYEKLNIVKPVKNKSIGWTIFAGIVLLGGTVRRNTYLFDFQLEEL
                     IVWSSC"
     gene            complement(457..>585)
                     /locus_tag="ACGEXS_002834"
     CDS             complement(457..>585)
                     /locus_tag="ACGEXS_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFF8013769.1"
                     /translation="ALLSMSYQNIIVYFAWITAIMGFFLVNIALIIDKNIHVILKN"
BASE COUNT          205 a           92 c           65 g          223 t
ORIGIN      
        1 aaacagctgc tccaaacaat tagttcttct aattgaaaat caaataaata agtatttctt
       61 cttacagtac caccaagtaa cacaataccc gcgaatatgg tccatcctat cgatttattt
      121 ttaactggtt tgacaatatt taatttttca taatcattct tagtgatttt gacatatgtt
      181 ttcggtatga gccagttaat aaatggaaag aagaagacaa tccaattact tgccaaatca
      241 atcattaaat attcactatc gtatttgatt attcgatatt tagggttttt attaataact
      301 ttagattcgc aaagcaatgt ctccacatcc ctttaatttt atgtgtaata catttttcga
      361 tacttcaaaa gacattcaaa tactatcaag ttactgtcat caaaggtttt attaactgat
      421 attttcatat ttttaatctg aatttagtac tttcacttaa tttttaagta tgacatgaat
      481 gtttttatct ataattaaag ctatattcac tagaaaaaaa cccattattg ctgtaatcca
      541 agcaaaatat actattatgt tttgataact cattgataat agagc
//
LOCUS       DAXWFK010000379          577 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.392, whole genome shotgun sequence.
ACCESSION   DAXWFK010000379 DAXWFK010000000
VERSION     DAXWFK010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.392"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..455
                     /locus_tag="ACGEXS_002835"
     CDS             <1..455
                     /locus_tag="ACGEXS_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HFF8013770.1"
                     /translation="MSKVDAHWELIEAIKNLRDEIAPNTLLTINDDIPDRKTGLELAE
                     KYGIDGIMIGRGIFHNPFTFEKEPREHTSKELLDLLRLHLSLFNKYEKDEIRQFKSLR
                     RFFKIYVRGIRGASELRHQLMNTQSIAEVRALLDEFEAQMDEDVKIEL"
BASE COUNT          210 a           86 c          115 g          166 t
ORIGIN      
        1 gcatgagtaa agtagatgca cattgggaat taatcgaagc tattaaaaat ttacgtgacg
       61 aaattgcacc aaacacattg ttaacaatta acgatgatat tcccgataga aaaacaggac
      121 ttgaacttgc agaaaaatat ggtattgatg gcatcatgat tggtagaggc attttccaca
      181 atccattcac ttttgaaaaa gaaccacgcg aacacacaag caaagaacta ttagatttgt
      241 tgagattgca tctatcattg tttaacaaat atgagaaaga tgaaatacga caattcaaga
      301 gtttgcgtag attctttaaa atctatgtgc gtggcataag aggcgctagc gaacttcgcc
      361 atcaattgat gaacacacaa tcaattgcag aagtacgagc actactagat gaatttgaag
      421 cccaaatgga cgaagacgtt aaaattgaat tatagtatga gtaataaggg aaaggaggaa
      481 tttaagtttc ctcctttgtt tgtgtgaaag tcttttaggt atgaactagg gtgatttaaa
      541 ataaaagttt aatttctatc ttcagttggc gtttcca
//
LOCUS       DAXWFK010000380          564 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.393, whole genome shotgun sequence.
ACCESSION   DAXWFK010000380 DAXWFK010000000
VERSION     DAXWFK010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.393"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            89..171
                     /locus_tag="ACGEXS_002836"
     tRNA            89..171
                     /locus_tag="ACGEXS_002836"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:122..124,aa:Leu,seq:gag)
     gene            194..267
                     /locus_tag="ACGEXS_002837"
     tRNA            194..267
                     /locus_tag="ACGEXS_002837"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:226..228,aa:Gly,seq:tcc)
     gene            386..>564
                     /locus_tag="ACGEXS_002838"
     rRNA            386..>564
                     /locus_tag="ACGEXS_002838"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          180 a          106 c          127 g          151 t
ORIGIN      
        1 aacgattaaa agttattgac aaatattttt aaacataata taataaataa cgttctgaaa
       61 aacgaagatg ttttaaatgc ctatatccgc ggtcgtggcg gaatggcaga cgcgctaggt
      121 tgagggccta gtgggagaaa tcccgtggag gttcaagtcc tctcggccgc atcaaaattc
      181 ttaatttaaa taagcgggtg tagtttaatg gcaaaacctc agccttccaa gctgatgttg
      241 tgggttcgat tcccatcacc cgctccatag ataattttaa tgaacattga aaactgaatg
      301 acaatatgtc aacgttaatt ccaaaaacgt aactataagt tacaaacatt atttagtatt
      361 tatgagctaa tcaaacatca taatttttat ggagagttcg atcctggctc aggatgaacg
      421 ctggcggcgt gcctaataca tgcaagtcga gcgaacggac gagaagcttg cttctctgat
      481 gttagcggcg gacgggtgag taacacgtgg ataacctacc tataagactg ggataacttc
      541 gggaaaccgg agctaatacc ggat
//
LOCUS       DAXWFK010000381          563 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.394, whole genome shotgun sequence.
ACCESSION   DAXWFK010000381 DAXWFK010000000
VERSION     DAXWFK010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.394"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            128..493
                     /locus_tag="ACGEXS_002839"
     CDS             128..493
                     /locus_tag="ACGEXS_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HFF8013771.1"
                     /translation="MKKKKNAIKVIIILFISLIVAVAFFFGLKTYQGHKNIQLIDSYL
                     EEKNLKDKIKSEKTEYSAKKGLFYKEVTFKDEPGVTYVVQPISTNKGLFVEGFDTETK
                     KSLKTAKHKYFNQNYKPSK"
BASE COUNT          231 a           62 c           88 g          182 t
ORIGIN      
        1 tcgtgtatgt aattcgattt ctataataat attgagaata aaaaaatttg ttacaatctg
       61 gtaaaaactc tttattaaat aaattgattc gttcatactg aatagataag caaaaaggag
      121 ttacataatg aagaagaaaa aaaacgcgat taaagttatc attatattat ttatttcatt
      181 aattgttgca gtagcatttt tctttggtct gaaaacatat caaggccata agaatattca
      241 actaattgac agttacttgg aagagaaaaa tctaaaagat aaaattaaat cagaaaaaac
      301 agagtatagt gctaaaaaag gtctcttcta taaggaagtt acatttaaag atgaacctgg
      361 tgtaacttat gttgtgcaac caatcagtac taataaagga ttatttgttg aaggctttga
      421 tacagagact aagaaaagtt tgaaaactgc aaagcataaa tatttcaatc aaaattataa
      481 accatcaaaa tagtgaaacg ttaaactggt gaagcagttt agtgccttca attttaatta
      541 tttgaggtaa acagtttttg tat
//
LOCUS       DAXWFK010000382          557 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.395, whole genome shotgun sequence.
ACCESSION   DAXWFK010000382 DAXWFK010000000
VERSION     DAXWFK010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.395"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
BASE COUNT          249 a           75 c           70 g          163 t
ORIGIN      
        1 taaataattt aaattaaaat aattaaactt tatgaaaatg gtaagcttag aaacgaaatt
       61 atacgagaat atgatctaac atcattaact ttctcaaatt agataaaaca aaaccaaaat
      121 actgagtcat tcaatcatca agacatttta acaagtgatg aaaaagagtt aataaaatta
      181 cgcaaagaaa aacatcgatt gataatgtaa aatgatgatt taaagcaagc agtgctaatt
      241 ttgagacaaa aaatgaagtc tttcaacaaa atgcgtatca atattcagta tcagcaatgt
      301 gcaaagttct aaaaatttca agaagtacct attatgattc taaaaaagga gaatattaca
      361 actagataca aaaagcctca atcacaacca agtaactaaa acgtaaaaat taattagatt
      421 aattattatc tgcataacaa atgaccatat acatcaacta gagacagact atttaatcat
      481 ttacaatgtc ctattcgttt tagaccatgt tcaaatgaat agtgtttata aaatgttgac
      541 actgtagtgg catattg
//
LOCUS       DAXWFK010000383          554 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.396, whole genome shotgun sequence.
ACCESSION   DAXWFK010000383 DAXWFK010000000
VERSION     DAXWFK010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.396"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..499
                     /locus_tag="ACGEXS_002840"
     CDS             <1..499
                     /locus_tag="ACGEXS_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFF8013772.1"
                     /translation="IKDKMDQLFITSEHFAIDIDKLLFQLNLNVRYIKMEGNTSGYLS
                     GRTIYINNNHSINNTRFSIARELGRYLFKNTDLMRDSNDNSLKNLHEMIYESDVNQFS
                     VDLLMPKKQIEALVYNFYEVNNINLSSGLSEKERNKLLNLISTKLEVSKVAAGLRLYN
                     LGIHI"
BASE COUNT          219 a           64 c           85 g          186 t
ORIGIN      
        1 tataaaggac aaaatggatc aactttttat tacttctgaa cactttgcta ttgacataga
       61 caagctatta tttcaactta atttaaatgt tagatatatt aaaatggagg gtaatacttc
      121 gggttattta agtggaagaa caatctatat taataataat cactcgatta ataatacaag
      181 attttcaata gctcgtgaat tgggccgtta tttatttaaa aatacagact taatgagaga
      241 tagtaacgat aatagtctaa agaatttaca tgaaatgatt tatgaaagcg acgtaaatca
      301 gttttcagtc gacttactta tgcctaaaaa gcaaatagag gcattagtct ataattttta
      361 tgaagtcaat aatatcaatt taagttctgg tttatctgaa aaagaacgaa acaaactatt
      421 gaaccttata tcaactaagt tggaagtttc gaaagtagct gctggattaa ggctgtataa
      481 tttaggaata catatttgat aaatattgtg aaaatctagt cgatttatta aaaccaaaag
      541 aaatttaata caat
//
LOCUS       DAXWFK010000384          548 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.397, whole genome shotgun sequence.
ACCESSION   DAXWFK010000384 DAXWFK010000000
VERSION     DAXWFK010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.397"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            271..548
                     /locus_tag="ACGEXS_002841"
                     /pseudo
     CDS             271..548
                     /locus_tag="ACGEXS_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          223 a           78 c           76 g          171 t
ORIGIN      
        1 tgtaatttta ctttgaaata ctttaaaaaa ataagacact ttgcccaact tgcacataaa
       61 tgtaaaattc aataaaataa attttctgtg ttggatccct tcgtataatt taataaatac
      121 tactaaacta aattaacgag gtgccttatg tataaaaatt ataacatgac ccaacttaca
      181 ctaccaatag aaacttctgt tagaattcct caaaatgata atacgcgata tgttaatgaa
      241 attgttgaaa cgatatctga tagcgaattc gatgaattta tatgtcctaa taacaaaaga
      301 ataggtttta aaagatatgc ataccgtaat gatagatata gttttaaacg tgacttcaag
      361 ctatatgaat gtgatgactg ttcatcatgt tctttgagac atcaatgcat gaagccaaat
      421 tcgaaatcca ataagaaaat tatgaagaat tataattggg aatactttaa agcccaaatt
      481 aatcaaaagc tttctgaacc agaaacgaaa aaatctatag tcaaagaaaa attgatgtag
      541 agcctgtt
//
LOCUS       DAXWFK010000385          545 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.398, whole genome shotgun sequence.
ACCESSION   DAXWFK010000385 DAXWFK010000000
VERSION     DAXWFK010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.398"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            91..>545
                     /locus_tag="ACGEXS_002842"
     CDS             91..>545
                     /locus_tag="ACGEXS_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein"
                     /protein_id="HFF8013773.1"
                     /translation="MKLKNIAKASLALGILTTGMITTTAQPVKASTLEVRSQATQDLS
                     EYYNRPFFEYTNQSGYKEEGKVTFTPNYQLIDVTLTGNEKQNFGEDISNVDIFVVREN
                     SDRSGNTASIGGITKTNGSNYIDKVKDVNLIITKNIDSVTSTSTSSTYT"
BASE COUNT          219 a           69 c           95 g          162 t
ORIGIN      
        1 gcacgaccat tgctcattat aatgaatgag gattgttcgt attgcgtaat agaataaatc
       61 aaatagacta aaaattggga gcatagaatt atgaaattaa aaaatattgc taaagcaagt
      121 ttagcactag ggattttaac aacagggatg attacaacta ctgctcagcc agtaaaagca
      181 agtacattag aggttagatc acaagctact caagacttga gtgaatatta taatagaccg
      241 ttctttgagt atacaaatca gtcaggatat aaagaggaag gaaaagtgac gtttactcct
      301 aattatcaac ttatagatgt aactttaact gggaatgaaa agcaaaattt tggtgaagat
      361 atttctaatg tagatatatt tgttgtaaga gaaaattctg atagatctgg taatacagct
      421 tcaattggtg gtattactaa aacaaacggt tcaaattata ttgataaagt aaaagatgta
      481 aatttaataa ttactaaaaa catcgatagt gttacatcaa cgtcaacatc atctacatat
      541 acaat
//
LOCUS       DAXWFK010000386          532 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.399, whole genome shotgun sequence.
ACCESSION   DAXWFK010000386 DAXWFK010000000
VERSION     DAXWFK010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.399"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
BASE COUNT          217 a           82 c           67 g          166 t
ORIGIN      
        1 atgttggatg tgaaagattg ttgatgagaa ttttgagatg tagataaata tcaaactagt
       61 ctataggtaa aaatttataa ttaatttgaa aagaatgtct caaataatgt ttgaattagc
      121 aggtgtcata ctaagtaatg ttaagtaaac attttatact ttatcagaac aaaaaagaca
      181 tgcatcaaca tgtcagtgaa actaatccat attatcttaa caacgaaatt gaatcgaacc
      241 tgaaaaacgt atttacaaat cgcaactact ctatccaagg caaaatcgca aaatctgatc
      301 cccgaaataa catatctcga aaaaaattcc ataattcaat catcttcaat atttaacaat
      361 acattactag ttccaaatat actcatacat acatgtttca ttgattgact gcatattgtc
      421 ataaataata ggggaactaa tcgctaataa tgttgttaaa cacaatacat agtaaagtga
      481 aagattataa atacatgaat tgttcaccat tccgccatat attttcaatc ac
//
LOCUS       DAXWFK010000387          482 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.401, whole genome shotgun sequence.
ACCESSION   DAXWFK010000387 DAXWFK010000000
VERSION     DAXWFK010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.401"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..>482
                     /locus_tag="ACGEXS_002843"
     rRNA            <1..>482
                     /locus_tag="ACGEXS_002843"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          136 a           91 c          147 g          108 t
ORIGIN      
        1 ctgggaggac catctcctaa ggctaaatac tctctagtga ccgatagtga accagtaccg
       61 tgagggaaag gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgct
      121 tacaagtagt cagagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga
      181 gttacgattt gatgcaaggt taagcagtaa atgtggagcc gtagcgaaag cgagtctgaa
      241 tagggcgttt agtatttggt cgtagacccg aaaccaggtg atctaccctt ggtcaggttg
      301 aagttcaggt aacactgaat ggaggaccga accgacttac gttgaaaagt gagcggatga
      361 actgagggta gcggagaaat tccaatcgaa cctggagata gctggttctc tccgaaatag
      421 ctttagggct agcctcaagt gatgattatt ggaggtagag cactgtttgg acgaggggcc
      481 cc
//
LOCUS       DAXWFK010000388          475 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.402, whole genome shotgun sequence.
ACCESSION   DAXWFK010000388 DAXWFK010000000
VERSION     DAXWFK010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.402"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(<1..299)
                     /locus_tag="ACGEXS_002844"
     CDS             complement(<1..299)
                     /locus_tag="ACGEXS_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFF8013774.1"
                     /translation="MLCESRQIYKNPKYRVIRYNNEYFMVDLVSTWITYFFPMINWFL
                     PKKYAKISENEFERLNIVEPVKNNVFWPVAGSSVLFGIILRKYGNFFNVQFEKQLA"
     gene            complement(346..>475)
                     /locus_tag="ACGEXS_002845"
     CDS             complement(346..>475)
                     /locus_tag="ACGEXS_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFF8013775.1"
                     /translation="VFENVQNLILYVSWLFMTMLFMFMNMHSIIDKKVHIFLKSNK"
BASE COUNT          182 a           78 c           49 g          166 t
ORIGIN      
        1 gctagttgtt tttcaaactg aacattaaag aagttaccgt actttctcaa tataattcca
       61 aatagaactg aacttcctgc aaccggccaa aaaacattat ttttaacagg ctcgactata
      121 tttaaccttt caaattcatt ttcgctaatt tttgcgtatt ttttgggcaa aaaccaatta
      181 atcataggga aaaaataagt aatccaagta cttactaaat cgaccatgaa atattcatta
      241 ttatatctaa taactcgata tttaggattt ttataaattt gtctagattc acaaagcact
      301 gtttaccacc cctaattaac aattgaaaac taactaaatt tgtaactatt tattagactt
      361 taagaatata tgtacttttt tatctataat tgaatgcata ttcataaaca taaatagcat
      421 agtcatgaaa agccaagaaa catataagat taaattttgc acattttcaa atacc
//
LOCUS       DAXWFK010000389          475 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.403, whole genome shotgun sequence.
ACCESSION   DAXWFK010000389 DAXWFK010000000
VERSION     DAXWFK010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.403"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..>475
                     /locus_tag="ACGEXS_002846"
     rRNA            <1..>475
                     /locus_tag="ACGEXS_002846"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          133 a           93 c          143 g          106 t
ORIGIN      
        1 ttaacttggg agtcagaaca tgggtgataa ggtccgtgtt cgaaagggaa acagcccaga
       61 ccaccagcta aggtcccaaa atatatgtta agtggaaaag gatgtggcgt tgcccagaca
      121 actaggatgt tggcttagaa gcagccatca tttaaagagt gcgtaatagc tcactagtcg
      181 agtgacactg cgccgaaaat gtaccggggc taaacatatt accgaagctg tggattgtcc
      241 tttggacaat ggtaggagag cgttctaagg gcgttgaagc atgatcgtaa ggacatgtgg
      301 agcgcttaga agtgagaatg ccggtgtgag tagcgaaaga cgggtgagaa tcccgtccac
      361 cgattgacta aggtttccag aggaaggctc gtccgctctg ggttagtcgg gtcctaagct
      421 gaggccgaca ggcgtaggcg atggataaca ggttgatatt cctgtaccac ctata
//
LOCUS       DAXWFK010000390          463 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.405, whole genome shotgun sequence.
ACCESSION   DAXWFK010000390 DAXWFK010000000
VERSION     DAXWFK010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.405"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            105..>463
                     /locus_tag="ACGEXS_002847"
     CDS             105..>463
                     /locus_tag="ACGEXS_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coagulase"
                     /protein_id="HFF8013776.1"
                     /translation="MKNKLLVLSLGALCVSQIWESNRASAVVSGEKNPYVSESLKLTN
                     NKNKSRTVEEYKKSLDDLIWSFPNLDNERFDNPEYKEAMKKYQQRFMAEDEALKKFFS
                     EEKKIKNGNTDNLDYLG"
BASE COUNT          187 a           36 c          100 g          140 t
ORIGIN      
        1 acagttaatt attttaaaaa ggtagatgtt atataatttg gcttggcgaa aaaatagggt
       61 gtaaggtagg ttgttaatta gggaaaatta aggagaaaat acagttgaaa aataaattgc
      121 tagttttatc attgggagca ttatgtgtat cacaaatttg ggaaagtaat cgtgcgagtg
      181 cagtggtttc tggggagaag aatccatatg tatctgagtc gttgaaactg actaataata
      241 aaaataaatc tagaacagta gaagagtata agaaaagctt ggatgattta atatggtcct
      301 ttccaaactt agataatgaa agatttgata atcctgaata taaagaagct atgaaaaaat
      361 atcaacagag atttatggct gaagatgagg ctttgaagaa attttttagt gaagagaaaa
      421 aaataaaaaa tggaaatact gataatttag attatctagg att
//
LOCUS       DAXWFK010000391          458 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.407, whole genome shotgun sequence.
ACCESSION   DAXWFK010000391 DAXWFK010000000
VERSION     DAXWFK010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.407"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..249
                     /locus_tag="ACGEXS_002848"
     CDS             <1..249
                     /locus_tag="ACGEXS_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin type 50 domain-containing
                     protein"
                     /protein_id="HFF8013777.1"
                     /translation="ILNNKKLPSYTILSIDLLNELLREKMSTGNLILSDERFDLKEII
                     NFNQIIGKIHIENVYIETRKGKVHYSKTGAHIVPYIDK"
     gene            260..454
                     /locus_tag="ACGEXS_002849"
     CDS             260..454
                     /locus_tag="ACGEXS_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013778.1"
                     /translation="MRIEDAYRKDVIITLLNNEEYEGFVTDYENEFESETGNLVVDIQ
                     TDFAVYSFDETEIKSIRLLK"
BASE COUNT          183 a           41 c           85 g          149 t
ORIGIN      
        1 attttaaata ataagaaatt gcctagctat acaatacttt ctatagattt attgaatgaa
       61 ttgttaagag aaaaaatgtc aacaggcaat ctaatattaa gtgatgagcg atttgatttg
      121 aaagagatta ttaattttaa tcaaattatt ggaaaaatac atatcgaaaa tgtgtatatt
      181 gaaaccagaa agggaaaagt gcattattcg aagacaggtg ctcatatagt accttatatt
      241 gataagtagg tgaaaagtat tgagaattga agatgcatat cgtaaagatg ttattatcac
      301 gcttttgaat aatgaagaat acgaagggtt tgtaactgac tatgaaaatg aattcgagag
      361 cgaaacagga aatcttgttg tagatataca gactgatttt gctgtttatt cgtttgatga
      421 aactgagata aaaagtatta gattattaaa ataattac
//
LOCUS       DAXWFK010000392          456 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.408, whole genome shotgun sequence.
ACCESSION   DAXWFK010000392 DAXWFK010000000
VERSION     DAXWFK010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.408"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            102..>456
                     /locus_tag="ACGEXS_002850"
     CDS             102..>456
                     /locus_tag="ACGEXS_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFF8013779.1"
                     /translation="MLCESKVINKNPKYRVIKYGDEYLMIDLVSTWLTLFLPMINWLI
                     PKKYVKISKKEFDDLNIVKPVKNKAFWPVAGSTILFGVTFRKYIPSLNIQLEKNMVIV
                     ICCAIFLGVLILFLFL"
BASE COUNT          169 a           45 c           71 g          171 t
ORIGIN      
        1 tgtgctcact atatgacaat aaagttcatg tattatttaa atcaaatgga attgaaaagt
       61 tttaatagga ataataaaca aaggaatggt agtgataaat attgctatgt gaatctaaag
      121 ttatcaacaa aaatcctaag tatagagtta ttaaatatgg tgatgaatat ttaatgattg
      181 atttagtaag tacctggtta actttatttc ttcctatgat taattggtta attccaaaaa
      241 aatatgtcaa aatcagtaaa aaagaatttg acgatttaaa cattgtcaaa cctgttaaaa
      301 ataaagcttt ttggccagtt gcaggtagta ctattttgtt cggagttaca tttagaaaat
      361 atattccttc acttaatatt caattagaga aaaacatggt gattgtaata tgttgtgcaa
      421 tatttctggg tgttttaata ctttttttat ttctga
//
LOCUS       DAXWFK010000393          450 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.409, whole genome shotgun sequence.
ACCESSION   DAXWFK010000393 DAXWFK010000000
VERSION     DAXWFK010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.409"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            175..330
                     /locus_tag="ACGEXS_002851"
     CDS             175..330
                     /locus_tag="ACGEXS_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013780.1"
                     /translation="MMVKKLLKAIKQNFLYEQSNITFKMIVFGYKRLSKMLFQFEINH
                     INFLWEG"
     gene            338..>450
                     /locus_tag="ACGEXS_002852"
     CDS             338..>450
                     /locus_tag="ACGEXS_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013781.1"
                     /translation="MINIISAIGSIGTFIMALFYFVSVSVQLYQMKISFLPA"
BASE COUNT          162 a           44 c           72 g          172 t
ORIGIN      
        1 tgttaagaaa ttacgtttgt aaaaataaaa aaatcaactt atttgtatga gataaatatg
       61 tattgaagaa gacgtgttat taatttggaa aatactggtc aaagatggga gctcttaaaa
      121 gcgttattgt attttttagt caatacaaat agattgccgt aataataatc gtacttgatg
      181 gttaaaaaat tacttaaggc tataaagcaa aactttttat atgagcagtc gaatataacg
      241 tttaaaatga ttgtttttgg atataaacga ttaagtaaaa tgctttttca gtttgaaatt
      301 aatcatataa atttcttatg ggagggttga tatcttaatg attaacatta tttcagctat
      361 aggatctatt ggaacattta ttatggcttt attttatttt gtatcagttt cagttcaact
      421 ttatcaaatg aaaattagct ttctgccagc
//
LOCUS       DAXWFK010000394          442 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.411, whole genome shotgun sequence.
ACCESSION   DAXWFK010000394 DAXWFK010000000
VERSION     DAXWFK010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.411"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..>442
                     /locus_tag="ACGEXS_002853"
     CDS             <1..>442
                     /locus_tag="ACGEXS_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1829 domain-containing protein"
                     /protein_id="HFF8013782.1"
                     /translation="ISLNKSKKRKKIFEEHLSAYGIKYNDKTHEIFVQTNFKNFNKSK
                     HNLLQCLIFVNDMYLLSNPKSQNIFTEDVANKLDEHNIYYGRDLPIIGSSGVVHNFDF
                     FISAKKNQKEKFINAISNPNNSMIIKSKITDAMQAKKIKRHRQN"
BASE COUNT          184 a           59 c           57 g          142 t
ORIGIN      
        1 gtatttcttt aaataaatca aaaaaacgta aaaagatttt tgaagagcac ctttcagctt
       61 acggtattaa atataacgat aaaactcacg aaatttttgt tcaaactaac tttaaaaatt
      121 ttaataaatc gaaacataat ttattacagt gccttatatt tgttaatgat atgtacttac
      181 tttctaatcc taagtcacag aacatattta cagaagatgt tgcaaacaaa ttggatgaac
      241 ataacattta ttacggaaga gatttaccta ttataggaag cagtggtgtt gttcataatt
      301 tcgacttttt tattagcgct aagaaaaatc aaaaagaaaa atttatcaat gctatttcta
      361 accctaataa ttctatgatt attaagtcga aaataacgga tgctatgcaa gcaaaaaaaa
      421 taaaaagaca caggcaaaat ga
//
LOCUS       DAXWFK010000395          439 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.412, whole genome shotgun sequence.
ACCESSION   DAXWFK010000395 DAXWFK010000000
VERSION     DAXWFK010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.412"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..>439
                     /locus_tag="ACGEXS_002854"
     CDS             <1..>439
                     /locus_tag="ACGEXS_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6119 family protein"
                     /protein_id="HFF8013783.1"
                     /translation="FKNNTLETFDATDPEVDYITEIGDYLLKYDVNSINDVRIEVIDN
                     LGHSNNMLLKTILHAEVEMEDGKKYLLQNGKWGYFNREFFDLLNDHLNEIEIRYNTLT
                     PTGLVFKEGEEGYIKEIVGRLPEEYLMLHKKFIKPINKNFIVKG"
BASE COUNT          188 a           40 c           79 g          132 t
ORIGIN      
        1 cttttaaaaa taatacatta gagacatttg atgcgactga tcctgaagtt gattacatca
       61 ctgaaatagg agattactta ttaaaatatg acgttaattc tataaatgat gtcagaattg
      121 aagtgataga taatttgggg cattcaaata atatgctact aaaaacgata ctacatgcag
      181 aagttgaaat ggaagatgga aagaaatatt tattacaaaa tgggaaatgg ggttatttta
      241 atagagaatt ttttgacctt ttgaatgatc atttgaatga gatagaaatt aggtataaca
      301 cactaactcc cacaggttta gtatttaaag aaggagaaga aggatatata aaagaaatag
      361 taggaagatt gccagaagaa tatttaatgt tacataaaaa attcataaaa ccaataaata
      421 agaattttat agtaaaagg
//
LOCUS       DAXWFK010000396          435 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.413, whole genome shotgun sequence.
ACCESSION   DAXWFK010000396 DAXWFK010000000
VERSION     DAXWFK010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.413"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(182..>435)
                     /locus_tag="ACGEXS_002855"
     CDS             complement(182..>435)
                     /locus_tag="ACGEXS_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8013784.1"
                     /translation="SDIYSLFKRLKHKTSINVHPHLFRHTHATVFYNETKDIKQVQER
                     LGHSNIQTTINLYVHPTEEDIREDWNKVKPQFQVFNKGE"
BASE COUNT          139 a           58 c           58 g          180 t
ORIGIN      
        1 tcttgaaatt gcttgaattt gttaaaagct gtaatgtgcc actcatcatt tttaaaaaag
       61 taatcatcat atatttcagt tattaaaacc tctcctttta tattgatggt tctactttgt
      121 aatgtacata attcattaat catagtactg tttgtaggta aattaattgg tattgacata
      181 gtcattcccc tttattaaaa acttgaaatt gaggctttac tttattccaa tcttcacgta
      241 tatcttcttc agtgggatga acatataaat taatagtcgt ttgtatatta ctatgaccaa
      301 gtctttcttg aacttgtttt atatccttag tttcattata aaaaacagtt gcatgtgtat
      361 ggcgaaataa atgaggatga acatttatag atgttttatg ttttagcctt ttaaataatg
      421 aatatatatc actat
//
LOCUS       DAXWFK010000397          424 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.415, whole genome shotgun sequence.
ACCESSION   DAXWFK010000397 DAXWFK010000000
VERSION     DAXWFK010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.415"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..>424
                     /locus_tag="ACGEXS_002856"
     CDS             <1..>424
                     /locus_tag="ACGEXS_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002500317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013785.1"
                     /translation="SKNMGFEVYYLANENEFTDDPFYEDVKIQEWLVNAPIFSFEGKS
                     KHFRQDGYSDNYINFLNKASVFVVWMDEQEGDVLEQCSKMALYFNSSANFFFLETMLN
                     ELQLYGFQQYNLNGKKEALYFRQWILNFKRSIAIIYRTE"
BASE COUNT          152 a           44 c           76 g          152 t
ORIGIN      
        1 tctaaaaata tgggttttga agtttattat ttagctaatg aaaatgaatt tactgatgat
       61 cctttctatg aggacgtgaa gattcaagag tggttagtaa atgcgcctat tttttcattt
      121 gaaggaaaaa gtaaacactt taggcaagat ggttatagcg ataattatat aaacttcttg
      181 aataaagcat ctgtttttgt agtttggatg gatgagcaag aaggagatgt tttagaacaa
      241 tgttctaaaa tggcacttta tttcaatagt tctgctaatt tctttttctt agaaactatg
      301 ttaaatgaat tacaattgta tgggtttcaa caatataatt taaatggaaa aaaagaagcc
      361 ctatatttta gacaatggat actaaatttc aagagatcta ttgcaattat ttatagaaca
      421 gagc
//
LOCUS       DAXWFK010000398          419 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.416, whole genome shotgun sequence.
ACCESSION   DAXWFK010000398 DAXWFK010000000
VERSION     DAXWFK010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.416"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..>419
                     /locus_tag="ACGEXS_002857"
     CDS             <1..>419
                     /locus_tag="ACGEXS_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HFF8013786.1"
                     /translation="CGIGKEAEVKKSFEKTLSMYPIKNLEDLYDKEGYRDDQFDKNDK
                     GTWIINSEMVIQPNNEDMVAKGMVLYMNRNTKTTNGYYYVDVTKDEDEGKPHDNEKRY
                     PVKMVDNKIIPTKEIKDEKIKKEIENFKFFVQYGDFK"
BASE COUNT          183 a           47 c           81 g          108 t
ORIGIN      
        1 ttgtggcata ggtaaagaag cggaagttaa gaaaagcttt gaaaaaacat tgagtatgta
       61 ccctattaaa aatctagagg atttatacga taaggaaggc tatcgtgatg atcagtttga
      121 taaaaatgat aaaggtacat ggattataaa ttctgaaatg gttattcaac ctaataatga
      181 agatatggta gctaaaggca tggttctata tatgaataga aataccaaaa caacaaatgg
      241 ttactactat gtcgatgtga ctaaggacga ggatgaagga aaaccgcacg acaatgaaaa
      301 aagatatccg gttaaaatgg tcgataataa aatcattcca acaaaagaaa ttaaagatga
      361 aaaaataaaa aaagaaatcg aaaactttaa gttctttgtt caatatggcg actttaaaa
//
LOCUS       DAXWFK010000399          418 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.417, whole genome shotgun sequence.
ACCESSION   DAXWFK010000399 DAXWFK010000000
VERSION     DAXWFK010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.417"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(1..339)
                     /locus_tag="ACGEXS_002858"
                     /pseudo
     CDS             complement(1..339)
                     /locus_tag="ACGEXS_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065796.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
BASE COUNT          110 a           79 c           53 g          176 t
ORIGIN      
        1 tccgcaattt atttctattt ttattttacc atttttaatc atttctttca tctggttttt
       61 agctttttcc tgtaattatg cttcttttac ttctacttga tatttacctt cacgctcttt
      121 aaagaacttg tcccgccaat tgccaacatg tggcactgtg gtacttctac accaaggatg
      181 cataggtggc gcattcacac ctggtatcat atctttaact ttaaatattt ttccgttgag
      241 tgaatgacat aatttagatg ttttactatc tattttggca acatatttat attcgccatc
      301 ttcaccaagt tcttttaaat atgttaactt ttgtgactct tctttttcaa cgaataatga
      361 aaaaagcata taaaaatagc atcgctttct ctttatctgt gtagaaagga tgctttaa
//
LOCUS       DAXWFK010000400          416 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.418, whole genome shotgun sequence.
ACCESSION   DAXWFK010000400 DAXWFK010000000
VERSION     DAXWFK010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.418"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            154..>416
                     /locus_tag="ACGEXS_002859"
     CDS             154..>416
                     /locus_tag="ACGEXS_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFF8013787.1"
                     /translation="MLCETENINKNPKYRIIKYKDEYLMIDLVSTWLALFFPMINWLI
                     PKKYVKISEKDFETLNIVKTAKINSFWPVAGSTVLFGVMLRRYS"
BASE COUNT          161 a           50 c           70 g          135 t
ORIGIN      
        1 aaagttcgcg ttataatgaa aaataattga ttacatttaa aatattctaa atgttgtcga
       61 cacaatcctt ttaagacgct agtagaattt aaatgacttc taatgtatat gaaagtgtat
      121 caatataaaa ccaattgaaa agaagtggag acattgcttt gtgaaactga aaatattaat
      181 aagaatccca aatatagaat tatcaaatac aaagatgaat atttgatgat tgatttagta
      241 agtacatggt tagcactctt tttcccaatg attaattggc tgattccaaa aaagtacgtc
      301 aaaatcagcg aaaaagattt tgaaacttta aacattgtga agacagctaa aatcaattct
      361 ttttggccag tggcaggaag tacggtctta tttggtgtta tgttaagaag gtattc
//
LOCUS       DAXWFK010000401          406 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.419, whole genome shotgun sequence.
ACCESSION   DAXWFK010000401 DAXWFK010000000
VERSION     DAXWFK010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.419"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            21..341
                     /locus_tag="ACGEXS_002860"
     CDS             21..341
                     /locus_tag="ACGEXS_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFF8013788.1"
                     /translation="MQSIKSNESFKSVINSDTPVIVKFEAGWCPDCRAMDLWIDPIVE
                     QYNDYQWYTVNRDELEDVVVENEVMGIPSLLVFKNGDKIAHLHSANAKSPEQVESFLA
                     ETFK"
BASE COUNT          147 a           59 c           74 g          126 t
ORIGIN      
        1 tatctagagg agttgttaca atgcaatcaa tcaaaagtaa tgaatcattt aaatctgtaa
       61 ttaatagcga tacacctgta attgttaaat ttgaggcagg atggtgccca gactgtcgtg
      121 ctatggattt atggattgac ccaatcgtag aacaatataa tgattaccaa tggtatactg
      181 ttaatcgtga tgaattagaa gatgtagttg ttgaaaatga agttatgggt atccctagct
      241 tgctagtatt taaaaacggc gataaaattg cacaccttca ttcagcaaat gctaagtcac
      301 ctgaacaagt tgaatcattt ttagcagaaa cttttaaata aatactaaaa taaaaatgtg
      361 tatatactgc acaataaaaa gctgagactg ttattgtacc gactct
//
LOCUS       DAXWFK010000402          405 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.420, whole genome shotgun sequence.
ACCESSION   DAXWFK010000402 DAXWFK010000000
VERSION     DAXWFK010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.420"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..277
                     /locus_tag="ACGEXS_002861"
     CDS             <1..277
                     /locus_tag="ACGEXS_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HFF8013789.1"
                     /translation="TINKFENIVSNEFVFYNASKITINDLSIKLKSAMANDQGITKHD
                     IGLAERAVYKVYFKNGSSKYVDLKTEYKDERVFKATDIKKVDIELKF"
BASE COUNT          162 a           44 c           70 g          129 t
ORIGIN      
        1 aacaataaat aaatttgaaa acatagtttc aaatgaattt gtgttctata atgcaagcaa
       61 aattacaatt aatgatttaa gtataaaact taaatcagca atggcaaatg atcaagggat
      121 aactaaacat gacataggac ttgctgaacg cgcagtgtat aaagtgtatt ttaaaaatgg
      181 ttcgtcaaaa tatgtagact taaaaactga gtataaagat gaaagagtat ttaaagcaac
      241 tgacattaaa aaggtagata ttgaacttaa attctaatct ttaattttat aataaggcat
      301 ctcacaatcg tggggtgcct tttacatttg tagagatgtg atgcttgaat gtttgaagtg
      361 atttgccaaa agttatataa attggttcta taataaatcg gactg
//
LOCUS       DAXWFK010000403          398 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.421, whole genome shotgun sequence.
ACCESSION   DAXWFK010000403 DAXWFK010000000
VERSION     DAXWFK010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.421"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..189
                     /locus_tag="ACGEXS_002862"
     CDS             <1..189
                     /locus_tag="ACGEXS_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFF8013790.1"
                     /translation="LVISATALKNSLPVLMFPDIWKGLLFIEVLLATVIVLRVFIGYL
                     HFFLKKEKQDKFLRNASQ"
BASE COUNT          134 a           67 c           55 g          142 t
ORIGIN      
        1 ttagtaatct cagcaactgc tttaaagaac agtttgcctg tacttatgtt tccagacatt
       61 tggaaaggtc ttttgtttat cgaagtgtta ttagccactg taatagtact tagagtcttt
      121 ataggatatc ttcacttctt tttaaaaaag gaaaaacaag ataaatttct tcgtaatgcg
      181 tctcagtaac actattacca agaattaaca cgtatattta ataactaaaa agaggcaggt
      241 aatcttgcct ctttttagtg tatatcaatg cctctttgat ttaatatgat cttatcaatg
      301 ataccttctg actaaagctg tagtaactat actagaacgt ctagttcata aacacgtttc
      361 actctaacct ttatttaaat aatatataag cagcaaac
//
LOCUS       DAXWFK010000404          387 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.422, whole genome shotgun sequence.
ACCESSION   DAXWFK010000404 DAXWFK010000000
VERSION     DAXWFK010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.422"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
BASE COUNT           98 a           85 c           51 g          153 t
ORIGIN      
        1 cttctctatt tatacaatat tttgttttcg atgatatcat tttaaattta tataaacaca
       61 aaattaaagt tgtacatact tagacaatcg aaagtgtaca ttattaaatt atcatttcca
      121 gttctactct agcggaacgt aagttggcta ccatcctcgc taagaacctt tcttgacttg
      181 tgacaatcgc ttgcttcttt cctctccttc ggctctcact tactcattta gctctactaa
      241 actcgttgcg ttcttttctc gtttcgtcag attcaaacgt tttcacttcg ccaagccatt
      301 tttctttgtg tttgcttttt attttgacgt tttagacata aaaaaaagag accttgcggt
      361 ctcaatgcgg ctcatcgcat ccatttt
//
LOCUS       DAXWFK010000405          387 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.423, whole genome shotgun sequence.
ACCESSION   DAXWFK010000405 DAXWFK010000000
VERSION     DAXWFK010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.423"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..>387
                     /gene="lpl8"
                     /locus_tag="ACGEXS_002863"
     CDS             <1..>387
                     /gene="lpl8"
                     /locus_tag="ACGEXS_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl8"
                     /protein_id="HFF8013791.1"
                     /translation="YIKRMALYMSVFLLIIFIVGCRNMKDEQKKEEQTNKTDSKEEQI
                     KKSFEKTLDMYPIKNLEELYDKEGYRDGEFKKGDKGMWTIYTDFAKSNKQGGLSNEGM
                     VLYLDRNTRTAKGHYFVKTFYNKGKFP"
BASE COUNT          165 a           41 c           79 g          102 t
ORIGIN      
        1 gtatataaaa agaatggctt tatacatgag tgtatttctt ttaatcattt ttattgttgg
       61 atgtcgaaat atgaaagatg aacagaaaaa agaagaacaa acaaataaaa cagattcaaa
      121 agaagaacaa atcaaaaaga gttttgagaa aacattagat atgtatccaa ttaagaatct
      181 cgaggagtta tacgacaaag aaggataccg agatggcgaa tttaaaaagg gtgataaagg
      241 gatgtggacg atatatacag atttcgccaa aagtaataaa caaggtggat tgagtaatga
      301 aggtatggtc ttatacttag atagaaatac acggactgca aagggacatt attttgttaa
      361 gacattctat aataagggca aattccc
//
LOCUS       DAXWFK010000406          371 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.424, whole genome shotgun sequence.
ACCESSION   DAXWFK010000406 DAXWFK010000000
VERSION     DAXWFK010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.424"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            20..370
                     /gene="scn"
                     /locus_tag="ACGEXS_002864"
     CDS             20..370
                     /gene="scn"
                     /locus_tag="ACGEXS_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN-A"
                     /protein_id="HFF8013792.1"
                     /translation="MKIRKSILAGTLAIVLASPLVTNLDKNEAQASTSLPTSNEYQNE
                     KLANELKSLLDELNVNELATGSLNTYYKRTIKISGQKAMYALKSKDFKKMSEAKYQLQ
                     KIYNEIDEALKSKY"
BASE COUNT          168 a           50 c           55 g           98 t
ORIGIN      
        1 ttactaagga gaataaaaaa tgaaaattag aaaatctata cttgcgggaa ctttagcaat
       61 cgttttagca tcaccactag taactaatct agataaaaat gaggcacaag ctagcacaag
      121 cttgccaaca tcgaatgaat atcaaaacga aaagttagct aatgaattaa aatcgttatt
      181 agatgaacta aatgttaatg aattagctac tggaagttta aacacttatt ataagcgaac
      241 tataaaaatt tcaggtcaaa aagcaatgta tgctcttaag tcaaaagact ttaagaaaat
      301 gtcagaagca aaatatcaac ttcaaaagat ttataatgaa attgacgaag cactaaaaag
      361 taaatattaa a
//
LOCUS       DAXWFK010000407          360 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.425, whole genome shotgun sequence.
ACCESSION   DAXWFK010000407 DAXWFK010000000
VERSION     DAXWFK010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.425"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
BASE COUNT          150 a           48 c           50 g          112 t
ORIGIN      
        1 gttttataac aacatatttt atgcaacaca taaaatgggt aactaacatc acgaaatcat
       61 tgtatttgtt gttacactga tgaagcgatg tcacaaagaa atgtcgttat caaacgaagc
      121 ccgaaataca tactgatact cgaaataatg taacgataaa taacaatgat aacgattgat
      181 tactacgcaa aaatatatgt aaattgacga tttaaatgca attcgtatat tttgccaatg
      241 atttacacat taaaatgttt gaaaattgtc acaattcagt ctaatttaaa aggaagttaa
      301 ttacaaaatt aaaaaatcat gcatcgtgtc atttagatat tgaaaaagat gagtcaaatt
//
LOCUS       DAXWFK010000408          329 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.428, whole genome shotgun sequence.
ACCESSION   DAXWFK010000408 DAXWFK010000000
VERSION     DAXWFK010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.428"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            <1..>329
                     /gene="fmhC"
                     /locus_tag="ACGEXS_002865"
     CDS             <1..>329
                     /gene="fmhC"
                     /locus_tag="ACGEXS_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhC"
                     /protein_id="HFF8013793.1"
                     /translation="NSVIVKMLGKIGYLHQGYTTGYSNKSQIRWISVLDLKDKDENQL
                     LKEMEYQTRRNIKKTIEIGVKVEDLSIEETNRFYKLFQMAEEKHGFHFMNEDYFKRMQ
                     EIYKDKA"
BASE COUNT          142 a           28 c           61 g           98 t
ORIGIN      
        1 ataattcagt gatagtaaag atgctaggga aaattgggta tctccatcaa ggttatacaa
       61 caggatattc aaataaaagt caaattaggt ggatttctgt attggattta aaagataaag
      121 atgagaatca acttttaaaa gaaatggaat accaaactag aagaaatata aaaaagacta
      181 ttgagattgg tgttaaggtt gaagatttat ctattgaaga aacaaatcga ttttataaat
      241 tgtttcaaat ggctgaagaa aaacatggtt ttcatttcat gaatgaagat tattttaaac
      301 gaatgcaaga aatatataaa gataaggca
//
LOCUS       DAXWFK010000409          322 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.429, whole genome shotgun sequence.
ACCESSION   DAXWFK010000409 DAXWFK010000000
VERSION     DAXWFK010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.429"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
BASE COUNT          119 a           56 c           57 g           90 t
ORIGIN      
        1 ggtgagggcc ccaacacaga agctgacgaa aagtcagctt acaataatgt gcaagttggg
       61 gatgggcccc aacaaagaga aattggattc ccaatttcta cagacaatgc aagttggggt
      121 gggacgacga aataaatttt gcgaaaatat catttctgtc ccactcccat caaaagaatg
      181 acattgaaaa aaatattaaa gtttggtgcc ataattttct ttctgctcaa tgaaataata
      241 cgtttaaaaa ttgaattgaa tcattcgcga aattgataat tattctcaat taataaaaaa
      301 tacttattac accttacctg ta
//
LOCUS       DAXWFK010000410          313 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.430, whole genome shotgun sequence.
ACCESSION   DAXWFK010000410 DAXWFK010000000
VERSION     DAXWFK010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.430"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
BASE COUNT          134 a           34 c           57 g           88 t
ORIGIN      
        1 acttagaggg atgttaatca taaaaaaaga gattttgaaa aatataggct attatacttt
       61 agaattacaa attataaatc ttgccagtgg atatgggagt tcaacatgtc acttacccca
      121 atttgtggaa tgttaaataa ctataaagag gaaaggagaa aaaatgataa gaatggttaa
      181 agaaaaagaa cgaatagcaa aattaaattt cgaatagctg tgacgagctt aataattcaa
      241 gtacttaatt ttatcaaatc gttcttttaa aaaggtaaag ggcaaaagac ctttaccaca
      301 ttaaataggg agg
//
LOCUS       DAXWFK010000411          278 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.denovo.433, whole genome shotgun sequence.
ACCESSION   DAXWFK010000411 DAXWFK010000000
VERSION     DAXWFK010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.denovo.433"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(24..>278)
                     /locus_tag="ACGEXS_002866"
     rRNA            complement(24..>278)
                     /locus_tag="ACGEXS_002866"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           69 a           76 c           63 g           70 t
ORIGIN      
        1 agatgttccg aatatatcct tagaaaggag gtgatccagc cgcaccttcc gatacggcta
       61 ccttgttacg acttcacccc aatcatttgt cccaccttcg acggctagct cctaaaaggt
      121 tactccaccg gcttcgggtg ttacaaactc tcgtggtgtg acgggcggtg tgtacaagac
      181 ccgggaacgt attcaccgta gcatgctgat ctacgattac tagcgattcc agcttcatgt
      241 agtcgagttg cagactacaa tccgaactga gaacaact
//
LOCUS       DAXWFK010000412         6681 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.guided.134, whole genome shotgun sequence.
ACCESSION   DAXWFK010000412 DAXWFK010000000
VERSION     DAXWFK010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6681
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.guided.134"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            134..1219
                     /locus_tag="ACGEXS_002867"
     CDS             134..1219
                     /locus_tag="ACGEXS_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8013794.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSKEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            1216..3108
                     /locus_tag="ACGEXS_002868"
     CDS             1216..3108
                     /locus_tag="ACGEXS_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFF8013795.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNVDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            3115..3492
                     /locus_tag="ACGEXS_002869"
     CDS             3115..3492
                     /locus_tag="ACGEXS_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6262 family protein"
                     /protein_id="HFF8013796.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            3643..4425
                     /locus_tag="ACGEXS_002870"
     CDS             3643..4425
                     /locus_tag="ACGEXS_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HFF8013797.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(4551..5282)
                     /gene="erm(A)"
                     /locus_tag="ACGEXS_002871"
     CDS             complement(4551..5282)
                     /gene="erm(A)"
                     /locus_tag="ACGEXS_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HFF8013798.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(5340..5399)
                     /locus_tag="ACGEXS_002872"
     CDS             complement(5340..5399)
                     /locus_tag="ACGEXS_002872"
                     /inference="COORDINATES: protein motif:HMM:NF019858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HFF8013799.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            5791..6453
                     /locus_tag="ACGEXS_002873"
     CDS             5791..6453
                     /locus_tag="ACGEXS_002873"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8013800.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
BASE COUNT         2404 a          901 c         1273 g         2103 t
ORIGIN      
        1 ttaaccatag tataataaat aatagtagtt aaagtagttt aagaggagat attaaaggga
       61 tattttactt taaataaaaa ttaacaaggt ttataatttg tagtagttaa agtatttaaa
      121 gaggtgggaa catgtgaagg ttcaaaggat agaagtggag aataagccgt atccattgta
      181 tttattacta gataaagaat accagctaat agaaccagta atgaaattta ttaaatactt
      241 agataatact ggtaagtctc ctaataccat taaggcatac tgctatcatt taaagttgct
      301 gtacgagttc atggaacaga gaggtgttat tcttaatgat attaactttg agttgttagc
      361 agacttcgta ggttggttga gatatccttc agcatcaaat gtaattgatc ttcagtcaaa
      421 aaaagccata agagaagaaa cgacagtgaa tacaatttta aatgtagtta tgagttttct
      481 tgattattta agtagattag gagaatttaa atcaattgat gtatttaaac aagcaaaggg
      541 aagaaatttc aaaggatttt tacatcatgt taataagggt agataccaaa agaatgtctt
      601 aaagttaagg gttaaaaaga aacagataag aacattgaga tcaaaggaag ttaagcaaat
      661 tattgatgct tgtcatacga aaagagataa attaatttta atgcttatgt atgagggtgg
      721 tttaagaatc ggtgaagtgt tatcgcttag gcttgaagat attgtcactt gggacaatca
      781 aatccattta acacctagag atgttaatgt taatgaagct tatattaaat taaggaagga
      841 aagaacaata catgtgagta aagaacttat gtcactttat acagattact tgatatatga
      901 gtatagtgag gaattggagc atgattatgt ttttatttcc ttaaaagaag gctattttgg
      961 gaaaccacta aagtaccaaa gtgttcttga tctagttaga agaatagtta aaaggactgg
     1021 aatagaattt acatcacata tgcttcgcca cactcacgca acgcagctaa ttagggaagg
     1081 atgggatgtt gcgttcgttc aaaagagatt aggtcacgca catgttcaga caacgttaaa
     1141 tacctatgtt catctttcag atcaggatat gaaaaatgag tttaataaat acctcgagag
     1201 aaaggagcat aagaaatgaa tgcttctagt aaaaggaaaa ttattagtca gagtgagatt
     1261 agcaaaaaaa tagctgtaat gaatgaagaa atgcaggggt tttgggctaa taatagttgg
     1321 gatataagaa aatgtccaca tccttctgcc atagaattaa gtaagaatcc tgctttaagg
     1381 aatcgttggg ttcgttttga acgtgttaaa aatctgtggt taagaacaga attgaaatat
     1441 ttttattttt accatttaaa caatggaata tggaatgcaa aaactgtctg gattagaaaa
     1501 ggaacagtaa ttaataaaat gttagatttc ttagatttaa agtatcctag cattacttca
     1561 attactgaag ttcctattga aaaagcaatg acggagtata gaacttattt gacaaaacgg
     1621 ggtgttagaa ttaccactac taattataag attactgcta atcaagaaaa aacacctgta
     1681 aaagctaatt cctactatgt tactaatcta aaacaattta tggagtttta tgagaacttt
     1741 tattttgatg gagaggagtg ggataaagac gtttgggata gacgtaactt acctttgcca
     1801 gatgataagg ttaacccaac acaatatgaa tatacaatta actttaaagg gtttcggaat
     1861 acatatttta aacaacttgt aaaaagatat tgtaagttga gattgaacgt ggatagcttt
     1921 tcctatgtaa gtgatattgc ccaaagactt aaagagttct ttaattttct ggacatgaaa
     1981 tttaaacaag ttcagagagt acaccaatta acgagagtgg aaattgaagc atatttaagt
     2041 gaactaaaca tgatgggaat aaaacctagt acaataactg ggaggatctc tatattggaa
     2101 ggactattta gtacccttct taggctagaa tgggatgatg ttccttccaa aatattaatt
     2161 tattctgagg actatccgaa aataccaaga gcaaaaccac gctttataga tgaattcgtc
     2221 ctagagcaat tgaacagtca tcttgataaa ttacctgaat atatagctac gatgactatg
     2281 attgttcaag aatgtggaat gaggataagt gaattgtgca ccttgaaaaa aggctgtcta
     2341 ttagaggaca aagatggaga tttcttttta aagtattatc aatggaaaat gaaaaaggag
     2401 catatagttc caatatctaa agaggtagct ttacttatta aagttcggga agataaagtt
     2461 tcagaggaat ttccagatag tgaatacctc tttccaagaa aagatggatc gccattaaaa
     2521 caagaaacat ttagaggtga gttaaataaa ttagcttatg agcaaaatat agtggataaa
     2581 tcaggtgaga tttatagatt ccatgcccat gcctttcgcc atacagtagg aacaagaatg
     2641 attaacaacg ggatgcccca gcatattgtg cagaaatttt tggggcatga aagcccagaa
     2701 atgacaagca gatacgctca tatctttgat gaaactctaa aaaatgaatt tactaaattt
     2761 caggaaaaac tggttaccaa taatggagat gtgcttgatc tagatgaaga taatgaagtc
     2821 gatgatgtag agcttcaatg gttcaagaaa aatataaatg cacaagtgct tccaaatggt
     2881 tattgtagat tgccagtagt agcaggtggt tgtccacatg cgaatgcatg cttagattgc
     2941 actcacttct gtaccagtaa gcaattctta ccacagcacg aagaacagtt agagcgtaca
     3001 gaagagttat tagccatagc taaggataaa caatggcaaa gacaagtaga gactaatagc
     3061 cgtgttaaag agcgtttaga acaaatcatt ggaagtttga cggggtaatt atcaatggat
     3121 aaacaagtta gaaatacaac agaaattgta cgtttggcga agcagaaatc aaaaaagaca
     3181 agggaaaaag tagacaaagc gatttctaaa ttttcgattg aaggtaaagt tattaatttt
     3241 aattcaatag caaaggaagc taatgtttct aaatcatggc tttataagga acacgatatt
     3301 aggcaaagaa tcgaatccct tcgtgagcgt caaataacag caaatgtagt ctcaaaaccc
     3361 aagaaaagtt ctcgttcgga ggaaatcctt attaaaacct taaaaagaag agtaatggaa
     3421 ttagaaaaag aaaataaaaa attacagaac caaattcaaa aattatatgg agatctgtat
     3481 aataaagaat aattattaat ctgtagacaa attgtgaaag gatgtactta aacgctaacg
     3541 gtcagcttta ttgaacagta atttaagtat atgtccaatc tagggtaagt aaattgagta
     3601 tcaatataaa ctttatatga acataatcaa cgaggtgaaa tcatgagcaa tttgattaac
     3661 ggaaaaatac caaatcaagc gattcaaaca ttaaaaatcg taaaagattt atttggaagt
     3721 tcaatagttg gagtatatct atttggttca gcagtaaatg gtggtttacg cattaacagc
     3781 gatgtagatg ttctagtcgt cgtgaatcat agtttacctc aattaactcg aaaaaaacta
     3841 acagaaagac taatgactat atcaggaaag attggaaata cggattctgt tagaccactt
     3901 gaagttacgg ttataaatag gagtgaagtt gtcccttggc aatatcctcc aaaaagagaa
     3961 tttatatacg gtgagtggct caggggtgaa tttgagaatg gacaaattca ggaaccaagc
     4021 tatgatcctg atttggctat tgttttagca caagcaagaa agaatagtat ttctctattt
     4081 ggtcctgatt cttcaagtat acttgtctcc gtacctttga cagatattcg aagagcaatt
     4141 aaggattctt tgccagaact aattgagggg ataaaaggtg atgagcgtaa tgtaatttta
     4201 accctagctc gaatgtggca aacagtgact actggtgaaa ttacctcgaa agatgtcgct
     4261 gcagaatggg ctatacctct tttacctaaa gagcatgtaa ctttactgga tatagctaga
     4321 aaaggctatc ggggagagtg tgatgataag tgggaaggac tatattcaaa ggtgaaagca
     4381 ctcgttaagt atatgaaaaa ttctatagaa acttctctca attaggctaa ttttattgca
     4441 ataacaggtg cttactttta aaactactga tttattgata aatattgaac aatttttggg
     4501 aagaataaag cgtcctcttg tgaaattaga gaacgcttta ttactttaat ttagtgaaac
     4561 aatttgtaac tattgaaaat agaaagaaat tgttccttcg atagtttatt aatattagtg
     4621 acatttgcat gcttcaaagc ctgtcggaat tggtttttag tgaaaagaac acgatattca
     4681 cggtttaccc acttataaac aaaagatcga tactttttgt agtccttctt tgaaatcaat
     4741 ggttgatgtc gttcaagaac aatcaataca gagtctacac ttggcttagg atgaaaatat
     4801 agtggtggta cttttttgag catttttata tccatctcca ccattaatag taaacccaaa
     4861 gctcgttgca gattttgcaa tcttttcgca aatcccttct caacgataag atagctatat
     4921 ttagcctgac tttcaaaggt aattcttttg acaatatccg tactgatgtt ataaggaata
     4981 ttaccatata tcttatagtt tatatgtttt gggaaggaaa attttagaat atccgtttga
     5041 atcactttta tattctcaga ggggtttacc gcttctttag tcacttgaca taagcctcca
     5101 tcaatttcta tagcagtaac tgatcgactc attttgacta gctctttggt aaaatgtcct
     5161 tttcctgatc cgatttctat tacgttgtct tgtttactga tattcgtgtg attcaatatt
     5221 tcttttacat gctttttaga agtaataaaa ttttgcgtgt ctttagggtt tttctggttc
     5281 attataacct tctccttact ggttataatg aactgacatt aagcgttcat tataaccaat
     5341 taattttgat ttggttgata acgaacttta ttaataacaa atatagaaaa agtacccata
     5401 cttttatctc ctttccattt ttttcattat gaatgagata aagtaatttc tactactgcg
     5461 atactggtgc acataattga aacctcctta tgtaactgaa tatgattgta tcatcttttc
     5521 attttagaat aaacctttat atgtaatttt gcacttgaca cttcagatgg acgttttctt
     5581 caaaataata tgttttgaaa cttgataaga gaagccactg tctttactaa aaagtaaagg
     5641 ctgcgataaa ggacagtgac tttaataaag ttgcactgtt gtattgtctt tggataaaat
     5701 tgtctaaagg gaagttttat ccccaacgaa aaaacatcta aagtggtaag taatgaaaga
     5761 atgtcgataa agtaggaatg ggggatcttt ttgaatacaa tagaatcaca gtttgataag
     5821 gttgcagaag attacgattt cgtgaatgag cttttgaatg attattcatt ctttgtgtct
     5881 aatatgtctc caaagaaagg cagagcatta gatatcggat gtggctcggg tttgttagtg
     5941 gagaaattag caagttatta tgatgaagtg gtagggattg atatttctaa tcaaatgctc
     6001 gatcttgcca aatctaaacg tcaactaaca aatacggtct atctgaatat gaatgcagaa
     6061 caacttaatt ttaatgagaa gtttgatttt attgtaagcc gaacaacctt tcatcatttg
     6121 gatgatatag ccagcgtaat acagcaaatg aaggaactgt tgaatgagga aggaagaata
     6181 gttattcttg ataatgtatc tgaagtcgag acaccgccta cctatgttta taaattaggg
     6241 gcaatccaag agtttttacc gcattgtttc aaatttggaa taaaaaatgc gattagaatt
     6301 tataatcaca acacatctaa atcatggctt gagcatttag cttcagataa gtacctatct
     6361 gaacaaaatt attacgattt atatgaaaag ttgttgcctg gatgccaatt ccataaaatg
     6421 ggttgggcaa tgggggttgt ctggacaaaa taacttaaag ctgtggcttt gaaaagttga
     6481 atttgtgatt gttttcagag ctatcgggtg ctttagtata aataacaatg aataaaaaat
     6541 cagcctgtat cttatgttac tattaagtaa tttaagatac aggttttact atttttgaaa
     6601 taagtacctt ccacttcaaa tacatgtatt caaagtgaaa gtattgattt aaatgattct
     6661 tttgtaattt actcttgata a
//
LOCUS       DAXWFK010000413         3206 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.guided.206, whole genome shotgun sequence.
ACCESSION   DAXWFK010000413 DAXWFK010000000
VERSION     DAXWFK010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.guided.206"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            complement(8..763)
                     /locus_tag="ACGEXS_002874"
     CDS             complement(8..763)
                     /locus_tag="ACGEXS_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8013801.1"
                     /translation="MEVVYVTQTQPKFLTIYNTLYKEIQIGKYPSGHALPTEKELCAR
                     FDVSRMTLRQAIKLLAEDGIVESTRGKGHFVIPQTNAQHASSMDQFKHPLDQILLARV
                     SMSSINYRVDLESEYTNHLFANHPSAVIAMERYYQKKDNHSKQADAFCFTFIPLNVID
                     TFKVNTQNEDDMKTFVEQTIYSNAYQSDLKMSITKAPHFKNHSHVFDGDTHCWLIIET
                     LYAQTPYPIMINKWYIPQEISELTLTRIRQSDY"
     gene            999..1706
                     /gene="deoD"
                     /locus_tag="ACGEXS_002875"
     CDS             999..1706
                     /gene="deoD"
                     /locus_tag="ACGEXS_002875"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFF8013802.1"
                     /translation="MKSTPHIKPMNDVEIAETVLLPGDPLRAKFIAETYLDDVEQFNT
                     VRNMFGFTGTYKGKKVSVMGSGMGMPSIGIYSYELIHTFGCKKLIRVGSCGAMQENID
                     LYDVIIAQGASTDSNYVQQYQLPGHFAPIASYQLLEKAVETARDKGVRHHVGNVLSSD
                     IFYNADTTASERWTRMGILGVEMESAALYMNAIYAGVEALGVFTVSDHLIHETSTTPE
                     ERERAFTDMIEIALSLV"
     gene            1713..3065
                     /gene="tet(38)"
                     /locus_tag="ACGEXS_002876"
     CDS             1713..3065
                     /gene="tet(38)"
                     /locus_tag="ACGEXS_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(38)"
                     /protein_id="HFF8013803.1"
                     /translation="MNVEYSKIKKAVPILLFLFVFSLVIDNSFKLISVAIADDLNISV
                     TTVSWQATLAGLVIGIGAVVYASLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFPLL
                     LVGRIIQTAGLAAAETLYVIYVAKYLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFIST
                     YLHWTNMFLIALIVVFTLPFLFKLLPKENNTNKAHLDFVGLILVATIATTVMLFITNF
                     NWLYMIGALIAIIVFALYIKNAQRPLVNKSFFQNKRYASFLFIVFVMYAIQLGYIFTF
                     PFIMEQIYHLQLDTTSLLLVPGYIVAVIVGALSGKIGEYLNSKQAIITAIILIALSLI
                     LPAFAVGNHISIFVISMIFFAGSFALMYAPLLNEAIKTIDLNMTGVAIGFYNLIINVA
                     VSVGIAIAAALIDFKALNFPGNDALSSHFGIILIILGLMSIVGLVLFVSLNRWTQSEK
                     "
     gene            3146..>3206
                     /locus_tag="ACGEXS_002877"
     CDS             3146..>3206
                     /locus_tag="ACGEXS_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8013804.1"
                     /translation="MKFEKYIDHTLLKPESTRTQ"
BASE COUNT         1014 a          486 c          577 g         1129 t
ORIGIN      
        1 ataattttta ataatccgat tgtcttatac gtgtcagtgt taattcagat atttcctgtg
       61 gaatatacca cttattaatc ataattggat aaggtgtttg tgcgtacagt gtttcaataa
      121 tcagccaaca atgtgtatca ccatcaaaca cgtgactatg atttttgaag tggggcgctt
      181 tggtaataga catttttaaa tctgattgat atgcattgct ataaatcgtt tgctcaacga
      241 atgtcttcat gtcgtcttcg ttttgtgtat tcactttaaa tgtgtcaatg acatttaacg
      301 gtataaaggt aaagcaaaat gcatcagctt gcttagaatg attgtccttt ttttgataat
      361 agcgttccat tgcaatgacg gcagaaggat ggtttgcaaa caaatgattt gtatattcac
      421 tttctaaatc aacacgataa ttaattgatg acatagatac gcgagctagc aatatttgat
      481 caagtggatg cttaaattga tccatacttg aagcgtgttg ggcatttgtt tgtggaataa
      541 caaagtgtcc cttccctctt gtactctcta cgatgccatc ttcggctaac aattttatag
      601 cttggcgcaa agtcatacga ctgacatcaa agcgcgcaca aagttccttt tcagtaggta
      661 atgcatggcc actcggatat tttcctattt gaatttcttt atataacgta ttataaatcg
      721 ttaaaaattt tggttgtgtt tgcgtcacgt agacaacctc cataaagtta cttaatcact
      781 ctcatcatac aataattttt actcaaattg gaaaaattat aaaaattaaa tatagatagg
      841 ctttgaaaat tagttttata caaggttagt agctgtaact gtaaaatgtt cttaatattg
      901 tcaaaatgta atgcttgaaa gcgcttttaa aaaatattat tatatacatg gttagacaaa
      961 tagacaaatc actatacaaa tattgggagg aatattttat gaaatcaaca ccacacatta
     1021 aaccaatgaa tgacgtcgaa attgcagaaa cggttctatt gccaggagat ccgttaagag
     1081 ctaagttcat tgcagaaact tatttggatg atgtggaaca gttcaataca gtgcgaaaca
     1141 tgtttggttt taccggaaca tataaaggta aaaaagtttc tgtcatgggt tcaggtatgg
     1201 gtatgccatc tattggcatt tactcttatg aattaattca tacatttggt tgtaaaaaat
     1261 taattcgcgt tggctcttgt ggcgcgatgc aagaaaacat tgatttatat gatgtgatta
     1321 ttgcacaagg tgcctctact gattcaaatt acgttcaaca atatcaatta ccaggtcatt
     1381 ttgcgccaat tgcttcttat caattattag aaaaagcagt tgaaacagca cgtgacaaag
     1441 gtgtacgtca tcatgtaggt aatgtgttat caagtgatat tttctataac gcggatacaa
     1501 cagcgagtga acgttggacg cgtatgggta ttttaggtgt agaaatggaa tcagctgcat
     1561 tatacatgaa tgcaatttac gctggtgtcg aagcattagg tgtgttcaca gtgagcgatc
     1621 atttaattca tgaaacgtca acaacacctg aggaaaggga acgtgcattt acagatatga
     1681 ttgaaattgc actgtcattg gtgtagatga ttatgaatgt tgaatattct aaaataaaga
     1741 aagcagtacc tattttatta ttcttatttg tattcagttt ggttatagac aactcattta
     1801 aattgatttc tgtagccatt gctgatgact taaacatatc tgtaacgaca gtaagttggc
     1861 aagcgacatt agccggttta gtaattggta ttggcgctgt agtatacgct tcattatctg
     1921 atgccattag tatacgcaca ctatttattt atggtgtgat attaatcatt atcggatcaa
     1981 ttattggtta cattttccaa catcaattcc cattactttt agttggacgt attattcaaa
     2041 ctgccggttt agctgctgca gagacattat atgtgatata tgttgcaaag tatctttcta
     2101 aagaggacca gaagacttac cttggcttaa gtacgagcag ttattccttg tcattagtta
     2161 tcggtacatt atcaggtgga tttatttcta cgtatttaca ctggacaaat atgtttttaa
     2221 ttgcattaat cgtagtattt acgttgccat tcctatttaa attattacca aaagaaaata
     2281 atacgaataa agctcattta gattttgttg gcttaattct agtggcaact attgctacaa
     2341 cagtcatgct gtttattacg aactttaatt ggttatatat gattggtgcc ttaattgcga
     2401 ttatcgtttt tgcgctatat attaaaaatg cgcaacgtcc attagtaaat aaatcatttt
     2461 tccaaaataa acgttatgct tcatttttat ttatagtatt tgtaatgtat gctatccaat
     2521 tgggttatat ttttacgttc ccattcataa tggagcaaat ttatcatctg caactagaca
     2581 caacatcact gttattagta ccgggttata tagtagcagt cattgttggt gcactaagtg
     2641 gtaaaatcgg cgaatatctg aattcaaaac aagcgattat cacagcaatt attttaatag
     2701 cactgagctt gattttacct gcatttgcag taggtaatca catttcaatc ttcgtcattt
     2761 ctatgatatt ctttgcaggt agctttgctt taatgtatgc acctttactt aacgaagcca
     2821 ttaaaacaat agatcttaat atgacaggtg tggctattgg tttttataat ttaattatta
     2881 atgtggcggt atctgtaggt attgcgattg ctgcggctct aatcgatttt aaagcattaa
     2941 atttcccagg caatgatgca ttaagttcac atttcggtat tattttaatt attttaggtt
     3001 taatgagtat tgtcggatta gttttattcg tcagcttaaa tcgttggaca caatctgaaa
     3061 aataaataga tataaattcg cgagatatat tcgtatttat agtaaaatta aataaagaga
     3121 ttatataaca cgaggagtag taagtatgaa atttgagaaa tatatagatc acactttatt
     3181 gaagcctgag tcaacacgta cgcaaa
//
LOCUS       DAXWFK010000414         1998 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.guided.258, whole genome shotgun sequence.
ACCESSION   DAXWFK010000414 DAXWFK010000000
VERSION     DAXWFK010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.guided.258"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            47..439
                     /locus_tag="ACGEXS_002878"
     CDS             47..439
                     /locus_tag="ACGEXS_002878"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8013805.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPNGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            440..1879
                     /locus_tag="ACGEXS_002879"
                     /note="aph(2'')-Ia"
     CDS             440..1879
                     /locus_tag="ACGEXS_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HFF8013806.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          832 a          153 c          342 g          671 t
ORIGIN      
        1 aacattgtat agcattttac acaggagtct ggacttgact gagtttatgg aagaagtttt
       61 aattgatgat aatatggttt ttgatattga taatttaaaa ggatttctta atgataccag
      121 ttcatttggg tttatagcta aagaaaataa taaaattata ggatttgcat attgctatac
      181 acttttaaga cctaatggaa aaacaatgtt ttatttacac tcaataggaa tgttacctaa
      241 ctatcaagac aaaggttatg gttcaaaatt attatctttt attaaggaat attctaaaga
      301 gattggttgt tctgaaatgt ttttaataac tgataaaggt aatcctagag cttgccatgt
      361 atatgaaaaa ttaggtggta aaaatgatta taaagatgaa atagtatatg tatatgatta
      421 tgaaaaaggt gataaataaa tgaatatagt tgaaaatgaa atatgtataa gaactttaat
      481 agatgatgat tttcctttga tgttaaaatg gttaactgat gaaagagtat tagaatttta
      541 tggtggtaga gataaaaaat atacattaga atcattaaaa aaacattata cagagccttg
      601 ggaagatgaa gtttttagag taattattga atataacaat gttcctattg gatatggaca
      661 aatatataaa atgtatgatg agttatatac tgattatcat tatccaaaaa ctgatgagat
      721 agtctatggt atggatcaat ttataggaga gccaaattat tggagtaaag gaattggtac
      781 aagatatatt aaattgattt ttgaattttt gaaaaaagaa agaaatgcta atgcagttat
      841 tttagaccct cataaaaata atccaagagc aataagggca taccaaaaat ctggttttag
      901 aattattgaa gatttgccag aacatgaatt acacgagggc aaaaaagaag attgttattt
      961 aatggaatat agatatgatg ataatgccac aaatgttaag gcaatgaaat atttaattga
     1021 gcattacttt gataatttca aagtagatag tattgaaata atcggtagtg gttatgatag
     1081 tgtggcatat ttagttaata atgaatacat ttttaaaaca aaatttagta ctaataagaa
     1141 aaaaggttat gcaaaagaaa aagcaatata taatttttta aatacaaatt tagaaactaa
     1201 tgtaaaaatt cctaatattg aatattcgta tattagtgat gaattatcta tactaggtta
     1261 taaagaaatt aaaggaactt ttttaacacc agaaatttat tctactatgt cagaagaaga
     1321 acaaaatttg ttaaaacgag atattgccag ttttttaaga caaatgcacg gtttagatta
     1381 tacagatatt agtgaatgta ctattgataa taaacaaaat gtattagaag agtatatatt
     1441 gttgcgtgaa actatttata atgatttaac tgatatagaa aaagattata tagaaagttt
     1501 tatggaaaga ctaaatgcaa caacagtttt tgagggtaaa aagtgtttat gccataatga
     1561 ttttagttgt aatcatctat tgttagatgg caataataga ttaactggaa taattgattt
     1621 tggagattct ggaattatag atgaatattg tgattttata tacttacttg aagatagtga
     1681 agaagaaata ggaacaaatt ttggagaaga tatattaaga atgtatggaa atatagatat
     1741 tgagaaagca aaagaatatc aagatatagt tgaagaatat tatcctattg aaactattgt
     1801 ttatggaatt aaaaatatta aacaggaatt tatcgaaaat ggtagaaaag aaatttataa
     1861 aaggacttat aaagattgat tatataatat atgaaaagct attataaaag acattagtat
     1921 taaatagttt aaaaaaatga aaaataataa aggaagtgag tcaagtccag actcctgtgt
     1981 aaaatgctat acaatgtt
//
LOCUS       DAXWFK010000415         1993 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.guided.259, whole genome shotgun sequence.
ACCESSION   DAXWFK010000415 DAXWFK010000000
VERSION     DAXWFK010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.guided.259"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            7..486
                     /locus_tag="ACGEXS_002880"
     CDS             7..486
                     /locus_tag="ACGEXS_002880"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFF8013807.1"
                     /translation="MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMG
                     RKTFESIGKPLPNRRNVVLTSDTSFNVEGVDVIHSIEDIYQLPGHVFIFGGQTLFEEM
                     IDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK"
     gene            501..1340
                     /gene="fakB2"
                     /locus_tag="ACGEXS_002881"
     CDS             501..1340
                     /gene="fakB2"
                     /locus_tag="ACGEXS_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB2"
                     /protein_id="HFF8013808.1"
                     /translation="MTKQIIVTDSTSDLSKEYLEANNIHVIPLSLTIEGASYVDQVDI
                     TSEEFINHIENDEDVKTSQPAIGEFISAYEELGKDGSEIISIHLSSGLSGTYNTAYQA
                     SQMVDANVTVIDSKSISFGLGYQIQHLVELVKEGVSTSEIVKKLNHLRENIKLFVVIG
                     QLNQLIKGGRISKTKGLIGNLMKIKPIGTLDDGRLELVHNARTQNSSIQYLKKEIAEF
                     IGDHEIKSIGVAHANVIEYVDKLKKVFNEAFHVNNYDINVTTPVISAHTGQGAIGLVV
                     LKK"
     gene            1426..1959
                     /gene="msrA"
                     /locus_tag="ACGEXS_002882"
     CDS             1426..1959
                     /gene="msrA"
                     /locus_tag="ACGEXS_002882"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFF8013809.1"
                     /translation="MTKEYATLAGGCFWCMVKPFTSYPGIKSVVSGYSGGHVDNPTYE
                     QVCTNQTGHVEAVQITFDPEVTSFENILDIYFKTFDPTDDQGQFFDRGESYQPVIFYH
                     DEHQKKAAEFKKQQLNEQGIFKKPVITPIKPYKNFYPAEDYHQDYYKKNPVHYYQYQR
                     GSGRKAFIESHWGNQNA"
BASE COUNT          729 a          306 c          353 g          605 t
ORIGIN      
        1 ataattatga ctttatccat tctagttgca catgacttgc aacgagtaat tggttttgaa
       61 aatcaattac cttggcacct accaaatgat ttgaagcatg ttaaaaaatt atcaacaggt
      121 catactttag taatgggtcg taagacattt gaatcgattg gtaaaccact accgaatcgt
      181 cgaaatgttg tacttacttc agatacaagt ttcaacgtag agggcgttga tgtaattcac
      241 tctattgaag atatttacca actaccgggc catgttttca tatttggagg gcaaacatta
      301 tttgaagaaa tgattgataa agtggacgac atgtatatta ctgttattga aggtaaattc
      361 cgtggtgata cgttctttcc accttataca tttgaagact gggaagttgc ctcttcagtt
      421 gaaggtaaac tagatgagaa aaatacaatt ccacatacct ttctacattt aattcgtaaa
      481 aaataagggg gaaaacgacc atgacaaaac agattatagt aacagactca acatccgatt
      541 tatctaaaga atacttagaa gcaaacaaca ttcatgtaat tcctttaagt ttaactattg
      601 aaggagcttc atacgttgac caagtagata ttacatcaga agaatttatt aatcatattg
      661 aaaatgatga agatgtaaag acaagtcagc cagccatagg tgaatttata tctgcttatg
      721 aagaactagg aaaagatggc tctgaaatca taagtattca tctttcttca ggattaagtg
      781 gtacatataa cactgcttac caagcaagtc aaatggtaga tgctaatgta actgttattg
      841 attcaaaatc tatttctttt ggtttagggt atcaaataca acacctagta gagcttgtaa
      901 aagaaggtgt ctcaacttct gaaatagtta aaaagttaaa tcatttaaga gaaaacatta
      961 aattatttgt agttataggg caattgaatc aattaattaa aggtggcaga attagtaaaa
     1021 caaaaggttt gattggtaat cttatgaaaa ttaaaccaat tggtacacta gatgatggtc
     1081 gcttagagct tgtgcacaat gcgagaactc aaaattctag tatccaatac ttgaaaaagg
     1141 aaattgctga atttatagga gatcatgaaa tcaaatccat tggtgtcgca catgctaacg
     1201 tcattgaata tgttgataaa ttgaagaaag tttttaatga agcttttcat gtgaataatt
     1261 acgatataaa tgtaactaca ccagttattt ctgcacatac tggtcaaggt gcgattggcc
     1321 tcgtagtcct taagaagtaa atttaatctt ttcagtgtta attacttcca tttcaatcct
     1381 ttatagacta aatttataat tagatagata gaggaggtaa ttcatatgac aaaagaatat
     1441 gcaacattag caggaggatg tttctggtgc atggttaaac catttacatc atatccaggc
     1501 atcaagtcag tcgtatctgg ttatagtggc ggtcatgttg acaacccaac ttatgaacag
     1561 gtatgtacga atcaaaccgg ccatgtcgaa gcagtacaaa ttacgtttga tccagaggtt
     1621 acttcctttg aaaatatatt agacatatat ttcaaaacat ttgacccaac tgatgatcaa
     1681 gggcaatttt tcgatagagg cgaaagctat caaccagtca ttttctatca tgatgaacat
     1741 cagaaaaagg ctgctgagtt taaaaagcaa caattaaatg aacaaggtat tttcaagaaa
     1801 ccagtgatta cacctattaa accatataaa aatttctatc cagctgaaga ctaccatcaa
     1861 gattattaca aaaagaaccc ggtacattat taccaatatc aacgtggttc aggtagaaaa
     1921 gcgtttatag aatcacattg ggggaatcaa aatgcttaaa aaagataaaa gtgaactaac
     1981 agatatagaa tat
//
LOCUS       DAXWFK010000416         1159 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.guided.325, whole genome shotgun sequence.
ACCESSION   DAXWFK010000416 DAXWFK010000000
VERSION     DAXWFK010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.guided.325"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            25..171
                     /locus_tag="ACGEXS_002883"
     CDS             25..171
                     /locus_tag="ACGEXS_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001807584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication domain protein"
                     /protein_id="HFF8013810.1"
                     /translation="MQQNQKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNY
                     FIKE"
     gene            331..1101
                     /gene="aadD1"
                     /locus_tag="ACGEXS_002884"
     CDS             331..1101
                     /gene="aadD1"
                     /locus_tag="ACGEXS_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-nucleotidyltransferase
                     ANT(4')-Ia"
                     /protein_id="HFF8013811.1"
                     /translation="MRIVNGPIIMTREERMKIVHEIKERILDKYGDDVKAIGVYGSLG
                     RQTDGPYSDIEMMCVMSTEEAEFSHEWTTGEWKVEVNFDSEEILLDYASQVESDWPLT
                     HGQFFSILPIYDSGGYLEKVYQTAKSVEAQTFHDAICALIVEELFEYAGKWRNIRVQG
                     PTTFLPSLTVQVAMAGAMLIGLHHRICYTTSVSVLTEAVKQSDLPSGYDHLCQFVMSG
                     QLSDSEKLLESLENFWNGIQEWTERHGYIVDVSKRIPF"
BASE COUNT          378 a          176 c          270 g          335 t
ORIGIN      
        1 taaaattata ctataaattc aactttgcaa cagaaccaaa aattaaacct tgatgacaca
       61 gaagaaggcg atttgattca tacagatgat gacgaaaaag ccgatgaaga tggattttct
      121 attattgcaa tgtggaattg ggaacggaaa aattatttta ttaaagagta gttcaacaaa
      181 cgggccagtt tgttgaagat tagatgctat aattgttatt aaaaggattg aaggatgctt
      241 aggaagacga gttattaata gctgaataag aacggtgctc tccaaatatt cttatttaga
      301 aaagcaaatc taaaattatc tgaaaaggga atgagaatag tgaatggacc aataataatg
      361 actagagaag aaagaatgaa gattgttcat gaaattaagg aacgaatatt ggataaatat
      421 ggggatgatg ttaaggctat tggtgtttat ggctctcttg gtcgtcagac tgatgggccc
      481 tattcggata ttgagatgat gtgtgtcatg tcaacagagg aagcagagtt cagccatgaa
      541 tggacaaccg gtgagtggaa ggtggaagtg aattttgata gcgaagagat tctactagat
      601 tatgcatctc aggtggaatc agattggccg cttacacatg gtcaattttt ctctattttg
      661 ccgatttatg attcaggtgg atacttagag aaagtgtatc aaactgctaa atcggtagaa
      721 gcccaaacgt tccacgatgc gatttgtgcc cttatcgtag aagagctgtt tgaatatgca
      781 ggcaaatggc gtaatattcg tgtgcaagga ccgacaacat ttctaccatc cttgactgta
      841 caggtagcaa tggcaggtgc catgttgatt ggtctgcatc atcgcatctg ttatacgacg
      901 agcgtttcgg tcttaactga agcagttaag caatcagatc ttccttcagg ttatgaccat
      961 ctgtgccagt tcgtaatgtc tggtcaactt tccgactctg agaaacttct ggaatcgcta
     1021 gagaatttct ggaatgggat tcaggagtgg acagaacgac acggatatat agtggatgtg
     1081 tcaaaacgca taccattttg aaggttctgt tgcaaagtaa aaaaatatag ctaaccacta
     1141 atttatcatg tcagtgttc
//
LOCUS       DAXWFK010000417          661 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Staphylococcus aureus strain KS015
            SAMD00636980-rid21400293.guided.375, whole genome shotgun sequence.
ACCESSION   DAXWFK010000417 DAXWFK010000000
VERSION     DAXWFK010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00636980
            Sequence Read Archive: DRR498609
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131548.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131548.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:52:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,885
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 12 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00636980-rid21400293.guided.375"
                     /strain="KS015"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
     gene            198..617
                     /gene="fosB"
                     /locus_tag="ACGEXS_002885"
     CDS             198..617
                     /gene="fosB"
                     /locus_tag="ACGEXS_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FosB/FosD family fosfomycin resistance
                     bacillithiol transferase"
                     /protein_id="HFF8013812.1"
                     /translation="MLKSINHICFSVRNLNDSIHFYRDILLGKLLLTGKKTAYFELAG
                     LWIALNEEKDIPRNEIHFSYTHIAFTIDDSEFKYWHQRLKDNNVNILEGRVRDIRDRQ
                     SIYFTDPDGHKLELHTGTLENRLNYYKEAKPHMTFYK"
BASE COUNT          245 a           94 c          101 g          221 t
ORIGIN      
        1 tacctttttc ctttaactta agttattagt gcctcttatg tagttgcgta gtcaactgta
       61 taccttttgc ctttaactta agttattaga gcctcttatg cagttgcgca gatcatcgta
      121 taaaaattaa tgacgtcatt tcaaaaatcg atacaaaaat aatttattat aaaaattcta
      181 agaaagaagt gaagcagatg ttaaaatcta ttaatcatat atgcttttca gtcagaaatt
      241 taaacgattc aatacatttt tatagagata ttttacttgg gaaattgcta ttgactggta
      301 aaaaaactgc ttattttgag cttgcaggcc tatggattgc tttaaatgaa gaaaaagata
      361 taccacgtaa tgaaattcac ttttcatata cacatatagc tttcactata gatgacagcg
      421 aatttaaata ttggcatcag aggttaaaag ataataacgt gaatatttta gaaggaagag
      481 ttagagatat tagagataga caatcaattt actttaccga ccctgatggt cataagctag
      541 aattacatac tggcacactt gagaacagat taaattatta taaagaggct aaaccacata
      601 tgacatttta caaataaggt gtcattataa aaaggcctct tgaactccgt taaaatttta
      661 a
//
